LOCUS       DAJTYF010000001       141759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTYF010000001 DAJTYF010000000
VERSION     DAJTYF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 141759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 141759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..141759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.001"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO590_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO590_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6635525.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO590_000002"
     CDS             complement(1672..2730)
                     /locus_tag="OO590_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6635526.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO590_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO590_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO590_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO590_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6635527.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO590_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO590_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6635528.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO590_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO590_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6635529.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO590_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO590_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6635530.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO590_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO590_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6635531.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO590_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO590_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6635532.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO590_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO590_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6635533.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFCCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO590_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO590_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6635534.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO590_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO590_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6635535.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO590_000013"
     CDS             complement(17699..18322)
                     /locus_tag="OO590_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6635536.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO590_000014"
     CDS             18619..18894
                     /locus_tag="OO590_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635537.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO590_000015"
     CDS             complement(18971..19213)
                     /locus_tag="OO590_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6635538.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO590_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO590_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6635539.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO590_000017"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO590_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6635540.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO590_000018"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO590_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6635541.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKACVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22592..24289
                     /gene="ypdA"
                     /locus_tag="OO590_000019"
     CDS             22592..24289
                     /gene="ypdA"
                     /locus_tag="OO590_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6635542.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24304..25037
                     /gene="ypdB"
                     /locus_tag="OO590_000020"
                     /pseudo
     CDS             24304..25037
                     /gene="ypdB"
                     /locus_tag="OO590_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
     gene            25050..25907
                     /locus_tag="OO590_000021"
     CDS             25050..25907
                     /locus_tag="OO590_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6635543.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="OO590_000022"
     CDS             complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="OO590_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6635544.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDLSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="OO590_000023"
     CDS             complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="OO590_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6635545.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="OO590_000024"
                     /pseudo
     CDS             complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="OO590_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30571..31818)
                     /locus_tag="OO590_000025"
     CDS             complement(30571..31818)
                     /locus_tag="OO590_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6635546.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31840..32166)
                     /locus_tag="OO590_000026"
     CDS             complement(31840..32166)
                     /locus_tag="OO590_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6635547.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32385..33350)
                     /gene="glk"
                     /locus_tag="OO590_000027"
     CDS             complement(32385..33350)
                     /gene="glk"
                     /locus_tag="OO590_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6635548.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33554..34810
                     /locus_tag="OO590_000028"
     CDS             33554..34810
                     /locus_tag="OO590_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6635549.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34925..35251
                     /locus_tag="OO590_000029"
     CDS             34925..35251
                     /locus_tag="OO590_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6635550.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35392..36630)
                     /locus_tag="OO590_000030"
     CDS             complement(35392..36630)
                     /locus_tag="OO590_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6635551.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36966..38168
                     /gene="nupC"
                     /locus_tag="OO590_000031"
     CDS             36966..38168
                     /gene="nupC"
                     /locus_tag="OO590_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6635552.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38218..40407)
                     /locus_tag="OO590_000032"
                     /pseudo
     CDS             complement(38218..40407)
                     /locus_tag="OO590_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40615..40690)
                     /locus_tag="OO590_000033"
     tRNA            complement(40615..40690)
                     /locus_tag="OO590_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40655..40657),aa:Ala,seq:ggc)
     gene            complement(40730..40805)
                     /locus_tag="OO590_000034"
     tRNA            complement(40730..40805)
                     /locus_tag="OO590_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40770..40772),aa:Ala,seq:ggc)
     gene            41026..41385
                     /locus_tag="OO590_000035"
     CDS             41026..41385
                     /locus_tag="OO590_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6635553.1"
                     /translation="MSKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41387..41779
                     /locus_tag="OO590_000036"
     CDS             41387..41779
                     /locus_tag="OO590_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6635554.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41831..43246)
                     /gene="gltX"
                     /locus_tag="OO590_000037"
     CDS             complement(41831..43246)
                     /gene="gltX"
                     /locus_tag="OO590_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6635555.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43505..43580
                     /locus_tag="OO590_000038"
     tRNA            43505..43580
                     /locus_tag="OO590_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43538..43540,aa:Val,seq:tac)
     gene            43625..43700
                     /locus_tag="OO590_000039"
     tRNA            43625..43700
                     /locus_tag="OO590_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43658..43660,aa:Val,seq:tac)
     gene            43705..43780
                     /locus_tag="OO590_000040"
     tRNA            43705..43780
                     /locus_tag="OO590_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43738..43740,aa:Lys,seq:ttt)
     gene            43811..43936
                     /locus_tag="OO590_000041"
     ncRNA           43811..43936
                     /ncRNA_class="other"
                     /locus_tag="OO590_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            44056..44397
                     /locus_tag="OO590_000042"
     CDS             44056..44397
                     /locus_tag="OO590_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6635556.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44388..45314)
                     /locus_tag="OO590_000043"
     CDS             complement(44388..45314)
                     /locus_tag="OO590_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6635557.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45404..46402
                     /locus_tag="OO590_000044"
     CDS             45404..46402
                     /locus_tag="OO590_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6635558.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46399..46617)
                     /locus_tag="OO590_000045"
     CDS             complement(46399..46617)
                     /locus_tag="OO590_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6635559.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46619..48634)
                     /gene="ligA"
                     /locus_tag="OO590_000046"
     CDS             complement(46619..48634)
                     /gene="ligA"
                     /locus_tag="OO590_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6635560.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48705..49592)
                     /gene="zipA"
                     /locus_tag="OO590_000047"
     CDS             complement(48705..49592)
                     /gene="zipA"
                     /locus_tag="OO590_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6635561.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRSMMT
                     PQKLREYQDIIREVKDANA"
     gene            49822..50583
                     /gene="cysZ"
                     /locus_tag="OO590_000048"
     CDS             49822..50583
                     /gene="cysZ"
                     /locus_tag="OO590_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6635562.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50768..51739
                     /gene="cysK"
                     /locus_tag="OO590_000049"
     CDS             50768..51739
                     /gene="cysK"
                     /locus_tag="OO590_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6635563.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52123..52380
                     /gene="ptsH"
                     /locus_tag="OO590_000050"
     CDS             52123..52380
                     /gene="ptsH"
                     /locus_tag="OO590_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6635564.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52425..54152
                     /gene="ptsI"
                     /locus_tag="OO590_000051"
     CDS             52425..54152
                     /gene="ptsI"
                     /locus_tag="OO590_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6635565.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54193..54702
                     /gene="crr"
                     /locus_tag="OO590_000052"
     CDS             54193..54702
                     /gene="crr"
                     /locus_tag="OO590_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6635566.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54744..55595)
                     /gene="pdxK"
                     /locus_tag="OO590_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54744..55595)
                     /gene="pdxK"
                     /locus_tag="OO590_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6635567.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55700..56074
                     /locus_tag="OO590_000054"
     CDS             55700..56074
                     /locus_tag="OO590_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6635568.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            56107..56830
                     /locus_tag="OO590_000055"
                     /pseudo
     CDS             56107..56830
                     /locus_tag="OO590_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(57034..57945)
                     /gene="cysM"
                     /locus_tag="OO590_000056"
     CDS             complement(57034..57945)
                     /gene="cysM"
                     /locus_tag="OO590_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6635569.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MHAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(58079..59176)
                     /gene="cysA"
                     /locus_tag="OO590_000057"
     CDS             complement(58079..59176)
                     /gene="cysA"
                     /locus_tag="OO590_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6635570.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(59166..60041)
                     /gene="cysW"
                     /locus_tag="OO590_000058"
     CDS             complement(59166..60041)
                     /gene="cysW"
                     /locus_tag="OO590_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6635571.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(60041..60874)
                     /gene="cysT"
                     /locus_tag="OO590_000059"
     CDS             complement(60041..60874)
                     /gene="cysT"
                     /locus_tag="OO590_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6635572.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60874..61890)
                     /gene="cysP"
                     /locus_tag="OO590_000060"
     CDS             complement(60874..61890)
                     /gene="cysP"
                     /locus_tag="OO590_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6635573.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62194..62985)
                     /gene="ucpA"
                     /locus_tag="OO590_000061"
     CDS             complement(62194..62985)
                     /gene="ucpA"
                     /locus_tag="OO590_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6635574.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(63172..63972)
                     /gene="murR"
                     /locus_tag="OO590_000062"
     CDS             complement(63172..63972)
                     /gene="murR"
                     /locus_tag="OO590_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6635575.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            64136..65032
                     /gene="murQ"
                     /locus_tag="OO590_000063"
     CDS             64136..65032
                     /gene="murQ"
                     /locus_tag="OO590_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6635576.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            65036..66460
                     /gene="murP"
                     /locus_tag="OO590_000064"
     CDS             65036..66460
                     /gene="murP"
                     /locus_tag="OO590_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6635577.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66640..67539)
                     /gene="yfeX"
                     /locus_tag="OO590_000065"
     CDS             complement(66640..67539)
                     /gene="yfeX"
                     /locus_tag="OO590_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6635578.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(67635..68210)
                     /locus_tag="OO590_000066"
     CDS             complement(67635..68210)
                     /locus_tag="OO590_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6635579.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68271..68720)
                     /locus_tag="OO590_000067"
     CDS             complement(68271..68720)
                     /locus_tag="OO590_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6635580.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68707..69132)
                     /locus_tag="OO590_000068"
     CDS             complement(68707..69132)
                     /locus_tag="OO590_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6635581.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69346..70215
                     /gene="amiA"
                     /locus_tag="OO590_000069"
     CDS             69346..70215
                     /gene="amiA"
                     /locus_tag="OO590_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6635582.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70219..71118
                     /gene="hemF"
                     /locus_tag="OO590_000070"
     CDS             70219..71118
                     /gene="hemF"
                     /locus_tag="OO590_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6635583.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(71052..72176)
                     /gene="eutR"
                     /locus_tag="OO590_000071"
     CDS             complement(71052..72176)
                     /gene="eutR"
                     /locus_tag="OO590_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR6635584.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITCQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSFG"
     gene            complement(72261..72722)
                     /gene="eutK"
                     /locus_tag="OO590_000072"
     CDS             complement(72261..72722)
                     /gene="eutK"
                     /locus_tag="OO590_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6635585.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            complement(72735..73394)
                     /gene="eutL"
                     /locus_tag="OO590_000073"
     CDS             complement(72735..73394)
                     /gene="eutL"
                     /locus_tag="OO590_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6635586.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(73404..74291)
                     /gene="eutC"
                     /locus_tag="OO590_000074"
     CDS             complement(73404..74291)
                     /gene="eutC"
                     /locus_tag="OO590_000074"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR6635587.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(74312..75673)
                     /gene="eutB"
                     /locus_tag="OO590_000075"
     CDS             complement(74312..75673)
                     /gene="eutB"
                     /locus_tag="OO590_000075"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCR6635588.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            75862..77067
                     /locus_tag="OO590_000076"
     CDS             75862..77067
                     /locus_tag="OO590_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6635589.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWINLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
     gene            77551..78321
                     /locus_tag="OO590_000077"
     CDS             77551..78321
                     /locus_tag="OO590_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6635590.1"
                     /translation="MRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQEQEQEQEQE
                     QEQEQEQEQNKAAAIAEFKELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNEE
                     AEEDLRFDSKEEQLPEEREQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDELK
                     LYTWMVSKCRYRGNDKYEIWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQALR
                     GKCGKTYRLRIPSKGTAEEASMTAQQHEREAKVSYLVH"
     gene            79372..80124
                     /locus_tag="OO590_000078"
     CDS             79372..80124
                     /locus_tag="OO590_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635591.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKQK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGYVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            80884..81279
                     /locus_tag="OO590_000079"
     CDS             80884..81279
                     /locus_tag="OO590_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6635592.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            81297..81518
                     /locus_tag="OO590_000080"
     CDS             81297..81518
                     /locus_tag="OO590_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635593.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLIC"
     gene            81583..82125
                     /locus_tag="OO590_000081"
     CDS             81583..82125
                     /locus_tag="OO590_000081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635594.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            82590..82796
                     /locus_tag="OO590_000082"
     CDS             82590..82796
                     /locus_tag="OO590_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635595.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            83108..83593
                     /locus_tag="OO590_000083"
     CDS             83108..83593
                     /locus_tag="OO590_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6635596.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            83574..83852
                     /locus_tag="OO590_000084"
     CDS             83574..83852
                     /locus_tag="OO590_000084"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635597.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            83845..85305
                     /locus_tag="OO590_000085"
     CDS             83845..85305
                     /locus_tag="OO590_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6635598.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            85339..86148
                     /gene="murI"
                     /locus_tag="OO590_000086"
     CDS             85339..86148
                     /gene="murI"
                     /locus_tag="OO590_000086"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6635599.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(86267..86958)
                     /locus_tag="OO590_000087"
     CDS             complement(join(86267..86712,86712..86958))
                     /locus_tag="OO590_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6635600.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALPPFNIGM
                     LTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEK
                     VIGAFIEKHMFY"
     gene            complement(87482..88855)
                     /gene="eutA"
                     /locus_tag="OO590_000088"
     CDS             complement(87482..88855)
                     /gene="eutA"
                     /locus_tag="OO590_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6635601.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(88830..89309)
                     /gene="eutP"
                     /locus_tag="OO590_000089"
     CDS             complement(88830..89309)
                     /gene="eutP"
                     /locus_tag="OO590_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6635602.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(89322..89657)
                     /gene="eutS"
                     /locus_tag="OO590_000090"
     CDS             complement(89322..89657)
                     /gene="eutS"
                     /locus_tag="OO590_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6635603.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(89950..92229)
                     /gene="maeB"
                     /locus_tag="OO590_000091"
     CDS             complement(89950..92229)
                     /gene="maeB"
                     /locus_tag="OO590_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6635604.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            92518..93468
                     /gene="tal"
                     /locus_tag="OO590_000092"
     CDS             92518..93468
                     /gene="tal"
                     /locus_tag="OO590_000092"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6635605.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            93488..95491
                     /gene="tkt"
                     /locus_tag="OO590_000093"
     CDS             93488..95491
                     /gene="tkt"
                     /locus_tag="OO590_000093"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6635606.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(95605..96636)
                     /locus_tag="OO590_000094"
     CDS             complement(95605..96636)
                     /locus_tag="OO590_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6635607.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQSFRLVRYAAEPTCDNW
                     HGPDARPTL"
     gene            complement(96762..97337)
                     /gene="nudK"
                     /locus_tag="OO590_000095"
     CDS             complement(96762..97337)
                     /gene="nudK"
                     /locus_tag="OO590_000095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6635608.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(97405..99384)
                     /gene="aegA"
                     /locus_tag="OO590_000096"
     CDS             complement(97405..99384)
                     /gene="aegA"
                     /locus_tag="OO590_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6635609.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDTPGVYDALPFIIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            99590..101313
                     /gene="narQ"
                     /locus_tag="OO590_000097"
                     /pseudo
     CDS             99590..101313
                     /gene="narQ"
                     /locus_tag="OO590_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            101515..104628
                     /gene="acrD"
                     /locus_tag="OO590_000098"
     CDS             101515..104628
                     /gene="acrD"
                     /locus_tag="OO590_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6635610.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNAAINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKAEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(104727..104786)
                     /gene="ypfM"
                     /locus_tag="OO590_000099"
     CDS             complement(104727..104786)
                     /gene="ypfM"
                     /locus_tag="OO590_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6635611.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            105167..105523
                     /locus_tag="OO590_000100"
     CDS             105167..105523
                     /locus_tag="OO590_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6635612.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            105527..106654
                     /gene="dapE"
                     /locus_tag="OO590_000101"
     CDS             105527..106654
                     /gene="dapE"
                     /locus_tag="OO590_000101"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6635613.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIIEQLVA"
     gene            106682..106882
                     /locus_tag="OO590_000102"
     CDS             106682..106882
                     /locus_tag="OO590_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6635614.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(106992..107690)
                     /gene="ypfH"
                     /locus_tag="OO590_000103"
     CDS             complement(106992..107690)
                     /gene="ypfH"
                     /locus_tag="OO590_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6635615.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(107764..109779)
                     /gene="tmcA"
                     /locus_tag="OO590_000104"
     CDS             complement(107764..109779)
                     /gene="tmcA"
                     /locus_tag="OO590_000104"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6635616.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAL
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGCLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(109794..110657)
                     /locus_tag="OO590_000105"
     CDS             complement(109794..110657)
                     /locus_tag="OO590_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6635617.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(110825..111538)
                     /gene="purC"
                     /locus_tag="OO590_000106"
     CDS             complement(110825..111538)
                     /gene="purC"
                     /locus_tag="OO590_000106"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6635618.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(111751..112785)
                     /gene="bamC"
                     /locus_tag="OO590_000107"
     CDS             complement(111751..112785)
                     /gene="bamC"
                     /locus_tag="OO590_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6635619.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(112802..113680)
                     /gene="dapA"
                     /locus_tag="OO590_000108"
     CDS             complement(112802..113680)
                     /gene="dapA"
                     /locus_tag="OO590_000108"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6635620.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            113826..114398
                     /locus_tag="OO590_000109"
     CDS             113826..114398
                     /locus_tag="OO590_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6635621.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            114398..114868
                     /gene="bcp"
                     /locus_tag="OO590_000110"
     CDS             114398..114868
                     /gene="bcp"
                     /locus_tag="OO590_000110"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6635622.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            115121..115738
                     /gene="hyfA"
                     /locus_tag="OO590_000111"
     CDS             115121..115738
                     /gene="hyfA"
                     /locus_tag="OO590_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6635623.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            115738..117755
                     /gene="hyfB"
                     /locus_tag="OO590_000112"
                     /pseudo
     CDS             115738..117755
                     /gene="hyfB"
                     /locus_tag="OO590_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            117766..118707
                     /gene="hyfC"
                     /locus_tag="OO590_000113"
     CDS             117766..118707
                     /gene="hyfC"
                     /locus_tag="OO590_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6635624.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGLASALLRLHGSSG"
     gene            118735..120174
                     /gene="hyfD"
                     /locus_tag="OO590_000114"
     CDS             118735..120174
                     /gene="hyfD"
                     /locus_tag="OO590_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6635625.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            120186..120836
                     /gene="hyfE"
                     /locus_tag="OO590_000115"
     CDS             120186..120836
                     /gene="hyfE"
                     /locus_tag="OO590_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6635626.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            120841..122421
                     /gene="hyfF"
                     /locus_tag="OO590_000116"
     CDS             120841..122421
                     /gene="hyfF"
                     /locus_tag="OO590_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6635627.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFFFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            122411..124125
                     /gene="hyfG"
                     /locus_tag="OO590_000117"
                     /pseudo
     CDS             122411..124125
                     /gene="hyfG"
                     /locus_tag="OO590_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            124135..124680
                     /gene="hyfH"
                     /locus_tag="OO590_000118"
     CDS             124135..124680
                     /gene="hyfH"
                     /locus_tag="OO590_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6635628.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            124677..125435
                     /gene="hyfI"
                     /locus_tag="OO590_000119"
     CDS             124677..125435
                     /gene="hyfI"
                     /locus_tag="OO590_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6635629.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            125428..125841
                     /gene="hyfJ"
                     /locus_tag="OO590_000120"
     CDS             125428..125841
                     /gene="hyfJ"
                     /locus_tag="OO590_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6635630.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            125871..127883
                     /gene="hyfR"
                     /locus_tag="OO590_000121"
                     /pseudo
     CDS             125871..127883
                     /gene="hyfR"
                     /locus_tag="OO590_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            127905..128753
                     /locus_tag="OO590_000122"
                     /pseudo
     CDS             127905..128753
                     /locus_tag="OO590_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(128791..129852)
                     /locus_tag="OO590_000123"
     CDS             complement(128791..129852)
                     /locus_tag="OO590_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6635631.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            130065..131528
                     /gene="bepA"
                     /locus_tag="OO590_000124"
     CDS             130065..131528
                     /gene="bepA"
                     /locus_tag="OO590_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6635632.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSNLLDEWAKGNVLQQRAAQYGRALQAMKANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            131549..131908
                     /gene="arsC"
                     /locus_tag="OO590_000125"
     CDS             131549..131908
                     /gene="arsC"
                     /locus_tag="OO590_000125"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6635633.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(132041..132787)
                     /gene="hda"
                     /locus_tag="OO590_000126"
     CDS             complement(132041..132787)
                     /gene="hda"
                     /locus_tag="OO590_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6635634.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(132837..134126)
                     /gene="uraA"
                     /locus_tag="OO590_000127"
     CDS             complement(132837..134126)
                     /gene="uraA"
                     /locus_tag="OO590_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6635635.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(134212..134838)
                     /gene="upp"
                     /locus_tag="OO590_000128"
     CDS             complement(134212..134838)
                     /gene="upp"
                     /locus_tag="OO590_000128"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6635636.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            135163..136200
                     /gene="purM"
                     /locus_tag="OO590_000129"
     CDS             135163..136200
                     /gene="purM"
                     /locus_tag="OO590_000129"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6635637.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            136200..136838
                     /gene="purN"
                     /locus_tag="OO590_000130"
     CDS             136200..136838
                     /gene="purN"
                     /locus_tag="OO590_000130"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6635638.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            137010..139076
                     /gene="ppk1"
                     /locus_tag="OO590_000131"
     CDS             137010..139076
                     /gene="ppk1"
                     /locus_tag="OO590_000131"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6635639.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            139081..140622
                     /gene="ppx"
                     /locus_tag="OO590_000132"
     CDS             139081..140622
                     /gene="ppx"
                     /locus_tag="OO590_000132"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6635640.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPSTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(140661..>141759)
                     /gene="pdeF"
                     /locus_tag="OO590_000133"
     CDS             complement(140661..>141759)
                     /gene="pdeF"
                     /locus_tag="OO590_000133"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6635641.1"
                     /translation="LSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDSHLK
                     QFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRCGAM
                     YLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELIGPD
                     SFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPVEVS
                     QLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARL
                     KNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAVLS
                     LGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        35404 a        36530 c        34910 g        34915 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgttgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagtggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaacaaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggtag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccctc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aatgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata ccccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ccgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgag atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggtgtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaagc cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccaaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgcccct gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacaga aaatttttct
    22381 acgctaacaa gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac
    23041 gttgaccatg attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt
    23101 ctctaaaatc ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct
    23161 ggtgcaaagc gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct
    23221 ggatatcgcc aacaaaacgc taccgctgtt tcgccatgtc aatagcgagt cattacgcaa
    23281 ggtctgcgaa attatccgcg atgacattca cgccgatgcg gtggcgatta ctaataccga
    23341 tcatgtgctg gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat
    23401 tagcccgact acccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga
    23461 agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggggt
    23521 cgtgaccgga acgctgaaaa tttactactg ccacgcgcat cagatcacct cgtcattaca
    23581 ggaaatggcg gtcggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga
    23641 gcagctacgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc
    23701 ccattttctg tttaacgctc tgaacgctat ttcatcgtca atccgtctga atccggatac
    23761 cgctcgccag ttgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga
    23821 cgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga
    23881 gcaggcccgc tttggcgaca agctgacggt tatctatgat attgatgaag aggtgaattg
    23941 ctgcattccc agcctgctga tccagccgtt ggtggagaac gccattgtcc acggtattca
    24001 gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggaa atcgggtacg
    24061 cattgcggtg cgagataccg ggcacggcat cgatccaaag gtgattgagc gggtcgaagc
    24121 gaatgaaatg ccgggcaata aaattggcct gctgaatgtc catcatcgcg tgaagttatt
    24181 gtatggcgag gggctgcata tccgccgcct ggagccgggg acggaaattg cgttttacat
    24241 tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccggagtgat
    24301 attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta
    24361 attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg
    24421 aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg
    24481 gatggcgtgt tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttc
    24541 atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt
    24601 ctcaaaccat atcaggagtc acgtattacc gggatgctgc aaaaactgga agcggcctgg
    24661 caacaacagc agaccagcgt acgcctgccg cgacggtaac gcgtgagaat gacaccatta
    24721 atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag
    24781 cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca
    24841 ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt
    24901 gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc
    24961 gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc
    25021 agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac
    25081 aatttttcgc cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct
    25141 ggtttgccag ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc
    25201 tggatatcgt gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg
    25261 tggaaggaga aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac
    25321 cggtgatgct gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata
    25381 gccgcaccac gtcgttgttg catcctgctc gccagatcca gcttcccagc ctgcaacgcg
    25441 gtgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga
    25501 atatcattca gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc
    25561 cgccgggtaa ttcgcaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc
    25621 aggataatta tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc
    25681 ccaatcatct gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc
    25741 gttgggtgcg aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg
    25801 aagtggcaca gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc
    25861 agtttggtct gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta
    25921 attctgtttc cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc
    25981 ggaagttttt attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc
    26041 cttcgttagc acccagcgtc agcatgatca ccagttcgac ttcgcccatt tctgactgcc
    26101 agtcaaccgg tttcgccagc cgggcaatgc tgatactgga atgacggatc cactgagatt
    26161 tggtgtgcgg gatcgctacg ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc
    26221 agacatcttc ttccagctca aacggatgtt cagtgcgccc gttaacgccg aggttgccgc
    26281 acaggaactg gatcgcctgc tctttattgc taagatcctg atcaacaaag atattctcca
    26341 gcgccagcag tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt
    26401 cctgcgcact gcggcattca catgcctgac gcgccagttc ccgacacgcc tcgctatcca
    26461 gttgacgaag ctggcttttc accgccggaa tacgcgggct actcatactc agctcatcca
    26521 ggcccagccc aagcagtagc ggcagataac ggctttcacc gcccagttca ccgcaaatgc
    26581 ctacccattt gccccgctga tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg
    26641 atggcgtaat cgggttatat agcggcgata cgcgcgggtt attacgatcg accgcataca
    26701 gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg cagaagtggt
    26761 cgatgatgta gcacaccgac ggcacttcca ccatgatccc aagcgtaatc gtctctgcat
    26821 gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcgcct ttcacccata
    26881 agatctgatc gaggctgtga accatcggga tcatcaactg ggcgttgccg aaactggcgg
    26941 cgcgcaaaat ggcccgcagt tgagtgcgga acaggccagc aaattccggg taaatacgta
    27001 ccgcgcgata gccgaggaat gggttctctt cctggggaac attcagataa gaaatgcttt
    27061 tatcgccgcc gatgtccatc gtgcggaaga taatcggctt gtcgcccgcc gccagtagca
    27121 cctgctggta ggcttcaaat tgctcctgct cgtccggcgc gctgtcgcga tccatataga
    27181 gcatttcggt acggaacagc ccgacacctt ccgcgccgtt ggcaaacgcg cctggcgctt
    27241 ccagagcggt gccgatattc gccgcaatat caatacgctt gttatcacgg gaataggcca
    27301 gctgcgcggc agcctgcgcc tgttgttttt ggcgtttatc cgccagcgtc tgcgcgacct
    27361 gataataacc gctcaccgcg tcattcgggt taatcgccag cacgccgcac tgggcgtcaa
    27421 gcactgcagg ttgcccggca taacgggcaa tcgcatccag cggcaagcca ctcagtaccg
    27481 ggatcgccga ggcacgggcc agaatcagtg tatgcgaggt gcgcccggtt ttctccagaa
    27541 tcatacccgc aagatttttc aaatcaaggc tcaaaaactg gcttggggtt aaatcttcag
    27601 ccaccagaat ggtcggtttt tccagcacca gattgttgcg cggcttcagt tccggccagg
    27661 tgatatgcag caactgttcg ctgatatcgc gaatgtcgct gacccgctcg cgcagataat
    27721 cgctggcaga agcagatagt ttggcgcaaa cctgctccat attgctgatg atcgccgccc
    27781 ccagtccctg atgctgttct gtcatcaggc gacggatatt gcctgcaaat tcatcatcct
    27841 gaatcagcga caaatgagcg ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt
    27901 gctgattcag ttgctcggca agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg
    27961 cactggcggg gattgcccga taactgtcga ggctgtcgct ttgtaacagg gtcagcgtac
    28021 ccacgccgac gccgcttgcc agcacattgc cgtacagtaa atccgggtta aggcggctta
    28081 atgaacgcgg cagcggatgc gccgtcagtt ctgcctgcgt cggctgaacg ctgtcgctgt
    28141 cgataaagcg caccaggatg tactcttcca gcacgcgccg cgcctgctct tcatcgctgc
    28201 cgctaatgtt caggctgcaa ctgtcattaa ataaggtgcc agttccaatc aacgccagcg
    28261 agcttttagc atcggccttt gcgttctggc gatggttgat aaaggtgatt tcgctttgcc
    28321 actggctgca ctgttcttta agttcccgcg ccggacgagc atgtagaccg ttaggcatag
    28381 gcaaaggaca gagaaattga atcgttaaca taaccgctcc gggttaatca ggagatcatc
    28441 tgaaatccgt cagttgaaca accgtctcac gcgtaagacg ttgaataagg gcagataaaa
    28501 gttgctccat ctgcaaaatg tcgcggcaat cggcaatcga tgccgcgcaa tgtccgtggc
    28561 gggtggcagg ccccattacc aatgtgggta cgccagtacc ggttaagtgc accgctccac
    28621 cgtccgtgcc gccgttactg aacatatccg cctgcaacgg aacgccaatt tctgccgcca
    28681 cggtttcaat ccacgcggta agttttggcg gcgcaatcag cgatttatcg cttaacacca
    28741 gcatcggccc gttaccaatc tgacgatggt tagccgcgcc ataatcaaag tttttcgccc
    28801 agcaggcggt atcaagcaca atggcgacgt ccggcgacac cgcgcgggta gcagtttgcc
    28861 cgccgcgtaa tcccacctct tcgctggaac ttgccaccag ccacacttcc gcaggcagtt
    28921 cggcgtcgtg cagctcacgt agtaacgtca ccagcagata gcaaccgagg cgatcatcaa
    28981 aggctttgcc catcacccgc tggtgaggga ggacctgaaa agtggtatcg aaggtgacgc
    29041 gatcgcctgg acgaattccc gcctgcatca cttcgtcata ggtgcgcgcg ccaatgtcca
    29101 cgcgcatggc gctaacgtca ttcccctgcc ggtcgccgtc aagcaggcct agaattttgc
    29161 actcttcacg ggtggtgatg cgcaccggct gcagctggcg ggcagccatg cgcacgttgc
    29221 caaccggcag cacgtcaata gcgccttcgc gggagatgct gcgcaccata aatcccactt
    29281 cgtccatatg ggcgcagatc atcaccttcg gacctgtcga ttcattgagg cggatcagca
    29341 ccgatcccag accatcaaat cgcacttctt tttgcaggcg agccgcttct tccagcagga
    29401 tctgccgcac ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac gcttttaata
    29461 gcgataaatc cattatgcct ctcccgtgag caacactgtt ttcggcatgg cgtagagcac
    29521 ttctgcgcct tgcggggtaa ccagcacaac atcttcgatg cgcacgcccc cttgccctag
    29581 caaataaatc cccggctcca cggtcagcaa catgcctggc tgtagcgtcg tggtgtcccg
    29641 cggtgaaaaa cgcggatctt catggacttc aatgccgata gcgtgaccag tgttatgacc
    29701 gaaatagtcg ccaaatccag actcggtaat gacccggcgc gcggcgtcgt caatctgctg
    29761 gcagcgcacg ccggggcgaa ttgcagagat tgccgcgagc tgtgcctgca ggacaatctg
    29821 atagacgtca aacagcgggt gagattcggc gctcacccct tcgccattca ccagcaaggt
    29881 gcgcgtcata tcagcagtag ccctgataca gcgcaccaaa atcgagagtg acaaactcgc
    29941 ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccgcgc cagccgctgg
    30001 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
    30061 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
    30121 cgcaatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
    30181 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
    30241 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
    30301 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
    30361 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
    30421 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
    30481 tctgccgtga ggagagaagc accgcatcca gttgttgcac cttaagccag ttgcgcagcg
    30541 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttgccttt
    30601 gcggaacatc atcagacgca ggaacaccac catcagcgca gtaatgattg cccccagcgc
    30661 gatccccgcc atataaacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
    30721 cgggaaccat tgcactgcgc ccagccagac agctgcggtt gagccgacaa tcgcgccgac
    30781 catatatgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
    30841 aatccccata aaggccagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
    30901 caggcgtttg ccggttaaac gacggtcaat aatggtcgcc agacccagac caatcggcgg
    30961 aataacgata gcgatagaac gagcggtgac cggtaatacg tggtcagtgg taaagctgaa
    31021 ggcaacgaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgctg tcgccgcggc
    31081 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cagtacggat
    31141 accgccgttg atccagccac caaagggggt gatgacgtag tacatcgcca gcatgacaaa
    31201 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
    31261 ctggattttc tggttcatcc atttcacaag gtaaccggca acgatagaaa taatcagcgc
    31321 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
    31381 gttcagcagt tgcgtcggct gggtagacat caacccgccg ataaaaccag ccgggaacgc
    31441 cagtttgcca ccgatagagt tcgccacaaa tgcagcaaac atcggaatgg caaaaccaaa
    31501 caacacgccg ccgaaggact gcgacagcca ggcaaattta agtaaggaga ggtcgaaacc
    31561 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
    31621 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
    31681 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
    31741 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
    31801 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
    31861 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
    31921 ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc ggggtaactg ccacggaatg
    31981 gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg cgattctgga tgccgtccgc
    32041 gccctgggtt tcaattttca cttcataacc ggcttctacc gccgcttctt ccagcgcctg
    32101 agcggccata aaagtgtgag ccaggcccat tgggcaggca cataaggcaa tcagtttctt
    32161 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
    32221 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
    32281 tgtgacgcag gtcggccttg tgaggggaca caaagaaaaa ttgaattaac tgtatgattt
    32341 aaaatattat cgggagagtt acctcccgat ataacaggaa ggatttacag aatgtgacct
    32401 aaggtctggc gtaaatgtgc accggaaccg agaagacccg gatagtcatg gacgatgaga
    32461 tacaccggaa tatcatggac atattcttta aagcgccctt tatcttcaaa tgcggcacgg
    32521 aaaccggagg ctttgaagaa ctcaaggaag cgcggcacga taccgcccgc aataaacacg
    32581 ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa aacggcccat aatgacgcaa
    32641 aacagcgaca atgcgcggcg gcaatcggtg cagctgtcag ccagcgcgcg ttcggtaata
    32701 tcttttggct tgagattttc tggcaggcgg ttgtcagctt tcacaattgc gcgatacaaa
    32761 ttcaccagcc cagggccaga aagcacgcgc tccgccgaaa catgaccaat ttccgcacgc
    32821 aatatttcga ggataatggc ctcttcttca ctattcggcg caaaatcaac gtgaccgcct
    32881 tcgcccggca agcttaccca acgcttatcg acatggacca gatgcgcaac cccaagcccc
    32941 gttccggcac cgtaaaccgc aataggctta ccttcgaccg gttctgcgcc accaaactga
    33001 atcagatgct cttttttcag catcgggatc gccatcgata cagcggtaaa atcgttaata
    33061 atttccagat ggctaaaacc gagattcttt ttcatttcgg caattgagaa cgcccaggta
    33121 tggttggtca ttgccaccca gtcaccggta attgggcaag cgatggcaat acagccgtct
    33181 ttcacctcga ccttatgttc ttcaagataa acgcgaatga ccgcttcgag gctggggtaa
    33241 tcaagccctg aataggtctt agcctgcgag atttcaccac tggcaatatc acacagagca
    33301 agacgtgcgt tggtgccgcc cacatcaccg actaatgcat actttgtcat tcttcaactg
    33361 ctccgctaaa gtcaaaataa ttctttctca cactgtaaat acttgggggc ataacaacaa
    33421 cgccggaaag gcaggctccc tgtaaatatc gatctgggtc acacaattac tttatcgttt
    33481 cagcaccaat tgcagcgatg cctttttgca agctgggcaa actaagtatc tgaccccgca
    33541 taaggaatag aacatgctcc atccgcgagc cagaaccatg ttgttattat cgctccccgc
    33601 cgtggcaatt gggattgcgt ccagtcttat tctgattgtg gtgatgaaaa tcgcctcggt
    33661 attacagaat ttgctctggc aacgactgcc gggaactctg gggatagccc aggattcccc
    33721 cctctggatc atcggtgtat taacgctaac gggtattgcg gtggggttgg ttatccgttt
    33781 cagccagggt catgccggac cagaccccgc ctgtgaaccg ctgatcggcg caccggttcc
    33841 gccctctgcg ctacctggac ttatcgtagc attaattctc ggtcttgctg gcggcgtcag
    33901 cctggggccg gaacatccga tcatgaccgt caatatcgcc cttgccgtcg ccattggcgc
    33961 tcgtctgtta ccgcgcgtca accgaatgga gtggactatt ttagcctctg ccggaaccat
    34021 cggcgcgctg tttggtacac ctgttgcggc ggcgttgata ttttcgcaaa ccttaaatgg
    34081 cagtagtgaa gttccgctat gggatcgtct ttttgcgccg ttaatggcgg cagcagctgg
    34141 tgcacttact accggattat ttttccatcc ccatttttca ctgcccattg cgcattacgg
    34201 acagatggaa atgaccgata ttctcagcgg tgcaattgtc gcggcgattg ccatcgcagc
    34261 agggatggtc gccgtatggt gcttaccacg gttgcacgcg ataatgaatc aaatgaaaaa
    34321 tccggtgctc gtgctgggta ttggcggatt tattctcgga attctggggg ttattggtgg
    34381 accagtttcg ctgtttaaag ggctggatga gatgcagcag atggtggcaa atcaggcttt
    34441 cagcaccagc gattactttt tgctggcggt aattaaactt gccgccctgg tcgttgctgc
    34501 cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg tttgtcggcg tggcattagg
    34561 gttgatgctg catgagcacg ttcccgccgt tccagcggca ataaccgttt cctgcgctat
    34621 tctcggcatc gtgctggtgg taacacgcga tggctggtta agtcttttta tggcggcagt
    34681 cgttgtaccc aataccacat tgctaccgct gctctgtatc gtcatgcttc cggcatggct
    34741 gttattagca ggtaagccga tgatgatggt caatcgtccg aagcaacagc caccccacga
    34801 taatgtttag caaaaaatgc tctcctttat gatttaagag ttatggcgat gatacggggc
    34861 tttacctccc cgtaatattg cgttaacagg ccgctgacgg cttcgatcgt gaaggagaat
    34921 aacaatgttc aggtcactgt ttctggcggc cgccctgatg gcatttaccc cgcttgcagc
    34981 aaatgcaggt gaaatcaccc tactgccatc gatcaaatta caaattggcg atcgcgatca
    35041 ttacggtaat tactgggacg gtggtcactg gcgagatcgt gattactggc atcgcaatta
    35101 tgaatggcgc aaaaaccgct ggtggcgtca tgataatggc tatcatcgtg gctgggataa
    35161 gcgtaaagca tatgagcgtg gctatcgtga aggctggcgc gatcgtgacg atcatcgcgg
    35221 aaaaggccgc ggacatgggc accgccatta aacgcgtcct ccaatggagc acaatgcctg
    35281 atgcgacgct agcgcgtctt atcaggccta caaaccggct ctttaacgcg tagggcggat
    35341 aaggcgttta cgccgcatcc gccaatagtg ccagatacga cgctcattca actacaatcc
    35401 cagcgccgtc cccaccaaca accagatatt cagcgccacg accagcacta caatcaccca
    35461 gcctgtctgt tttacgcgtt tgctgttcac cagatcgccc atcaacttgc tgtcactggt
    35521 gaaaatcagc agtggaacca gcgccagggc gataccaaaa cttaacagca cctgactcat
    35581 aaccagaatc cgtgtcggat ctaatcccat cagaatgaca ataaatgacg gcaacatggt
    35641 gactgtacga cgcacccaca gcgggatatg gaagcgaatg aatccctgca tcaccacctg
    35701 ccccgccagt gtccccacca ccgttgagga cagtccggca gcaaccagac ttaacccaaa
    35761 gaccgttgcc gcagcatggc ttaacagcgg ttgcagcgtc agataagcct catcaagatc
    35821 ggcaacacca gtatgaccag aaaagtggaa cgccgccgca gctgtagcca tcatcgccag
    35881 attgacaaaa ccggcaatcg tcatggcgat agccacatcc catttggtgg cggaataacg
    35941 ttgttgacgc gaaccgccat gtaaatgctg agtgagcgaa gagtgcaaat aaatcacatg
    36001 cggcataatc gtcgccccta acacgcctgc tgccaggaag accgcctccg aagtaggtaa
    36061 actcgggatc accattcctt tacccagctg cgccaggtta ggctgggaga aaatcaactc
    36121 gacaatgtaa gccgcggcaa caaacaacag taacccgcca atcactttct ccagcggttt
    36181 ttgcccgcga cgttgcagca ttaaaatcag gaaagtcgcg atccccgtca gcaccgcgcc
    36241 ctgcaacaac gaaacaccaa gaatgagttt aaaaccgatc gccgcaccaa taaattccgc
    36301 caggtcggtt gccatcgcaa taatttctgc ctgaacccaa tagaaccaca ctacgggacg
    36361 cggatagtga tcacgaatct gctccgccag atttttaccg gtggcaatcc ctagtttggc
    36421 agagaggatc tgaattagca tcgccatcag gttggcccaa acgacaaccc acagtagctg
    36481 atagccgaag ctagcacccg cctgaatatt ggtcgcaaag ttaccgggat cgatataacc
    36541 aatcgccgca atgaacgcag gtcccattaa tgcgagcctc atcttgcgcg ccgcccgtcc
    36601 gctgctactc tcaacgcgat agttcgtcat cttgtgcctc taaaacatag cctttgctat
    36661 gtttcatgct attctaaacg agaatgatta tcaaattcat ttaaatggat tgtggtgatt
    36721 tctctgatag accaagatta cgattacgaa aagattgatg acttgaatgt tatggttatg
    36781 tttaatgtta gcacatttac ataactttca gcttccatac acaacatagc agaaatgtat
    36841 gacagatcac tatttttgaa gcctgtcaca ggacgtcatt atagtgtgtg tcagatctcg
    36901 ttttccttaa ccatgttaca tagaatgtgc acggaaattt aacctgcctc atatttggag
    36961 caaatatgga ccgcgtcctt cattttgtac tggcacttgc cgttgttgcg attctcgcac
    37021 tgctggtaag cagcgaccgc aaaaaaattc gtatccgtta tgttattcaa ctgcttgtta
    37081 tcgaagtgtt actggcgtgg ttcttcctga actccgacgt tggtttaggc ttcgtgaaag
    37141 gcttctccga aatgttcgaa aaactactcg gatttgccaa cgaagggact aacttcgtct
    37201 ttggtagcat gaatgatcaa ggcctggcat tcttcttcct gaaagtgctg tgcccaatcg
    37261 tctttatctc tgcactgatc ggtattctcc agcacattcg cgtgttgccg gtgattatcc
    37321 gcgcaattgg tttcctgctc tccaaagtca acggtatggg caaactggaa tcctttaacg
    37381 ccgtcagctc cctgattctg ggtcagtcag aaaactttat tgcctataaa gatatcctcg
    37441 gcaaaatctc ccgtaatcgt atgtacacca tggctgccac ggcaatgtcc accgtgtcga
    37501 tgtccatcgt tggtgcatac atgaccatgc tggaaccgaa atacgtcgtt gctgcgctgg
    37561 tactgaacat gttcagcacc tttatcgtgc tgtcgctgat caatccttac cgtgttgatg
    37621 ccagtgaaga aaacatccag atgtccaacc tgcacgaagg tcagagcttc ttcgaaatgc
    37681 tgggtgaata cattctggca ggtttcaaag ttgccattat cgttgccgcg atgctgatcg
    37741 gctttatcgc cctgatcgcc gcgctgaacg cactgtttgc caccgttact ggctggtttg
    37801 gctacagcat ctccttccag ggcatcctgg gctacatctt ctatccgatt gcatgggtga
    37861 tgggtgttcc ttccagtgaa gcactgcaag tgggcagtat catggcgacc aaactggttt
    37921 ccaacgagtt cgttgcgatg atggatctgc agaaaattgc ttccacgctc tctccgcgtg
    37981 ctgaaggcat catctctgtg ttcctggttt ccttcgctaa cttctcttca atcgggatta
    38041 tcgcaggtgc agttaaaggc ctgaatgaag agcaaggtaa cgtggtttct cgcttcggtc
    38101 tgaaactggt ttacggctct accctggtga gtgtgctgtc tgcgtcaatc gcagcactgg
    38161 tgctgtaaaa ccatacataa aaaagccggg gatcattccc cggctttttt acgcctgtta
    38221 atcagctaat ggctgcgggc gaccaatcaa atacccttgc agatattgca ccccgagctt
    38281 atgcaatagc gcctgctgct gttgcgtctc gacaaactcc gcgaccacac tcaatgactt
    38341 cgctttcgcc agatcggtaa ttgatctcac aatcatcgca tccagcgtat tcgtgacaat
    38401 atctttcaca aagacgccat caattttgat gatatcagcc tgcaaacgct ttaaccgttc
    38461 gtagttggca tatccggtgc caaagtcatc aatcgcaatc cggaaaccaa acttatgcag
    38521 ctgctcgatg ttgtacatgc tgctttctgc gttagaaaac gcctgctcct cggtgatctc
    38581 aagaatgacc gcctgcgggg agatgtgata acgcttaaac agacgaataa tccgtccggc
    38641 aatattcttt tgcagcagcg tgagcggcat taaattgact gaaaagcgcg gtcctttttt
    38701 gtcgcaaggg tgtgttgcca gccacttcaa caaggattcc agcacttgca aatcaaaacg
    38761 cgcgctaagg ttaaactgag caataagggg cagaaactta tccggggtca taatgccgcc
    38821 gtcatatttc agtcgcgcga ggatctcatc ataaccttca ccctctttgt tgcgaattgg
    38881 ctgggcgtag agcagcaaat caccctgatc taacgccgtg cgaatggtat tcagcaatag
    38941 cacctgttta gtggtctgcc cggaaaccat ctcctctctg ctatccagcg ccagcacatg
    39001 atgatgtgcg caggattgct ccgccagcca gcttaactgc cccaacaagg gttgcagggt
    39061 ttcctgattt ccatcaaaac gcccccaggc agcaccatag cccatatcca gcccggtatt
    39121 gttccagtga atttgccgac tattcaggat gttaaccata tgctggagtc gcccttccgt
    39181 ttccggcccg ctcagcacta acagcagttc actacccggc aattgataca acttttcgtt
    39241 ttcctgcatc aacggcagca gcgtacggca aattgagcgg atacagtgaa cgcgcatcat
    39301 taagccgtaa tgacgactca taaactcaag attatcaatg cgcaggcagc aaaaactctt
    39361 gcccgcctct tgctccggcg cttgttccaa cgcacgaaag ttgggtagga gcgttaacgg
    39421 atctgttagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    39481 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcgagaataa atgccagcga
    39541 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    39601 aacagcctag gtgagattta aaaacggata gcgaagatta ccgaccccga gggtaaagat
    39661 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    39721 cagaagtagc agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    39781 gcccaacgac ggagcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    39841 catgcgagtg agatagtaga aaagcatgtt gtaaatcagc actgcggtga aaaggcttag
    39901 taaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    39961 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    40021 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    40081 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctgccgag
    40141 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    40201 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    40261 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    40321 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    40381 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    40441 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca ggggccagcc tgttgccgat
    40501 ttttcatcca atgatgcttt caccaggcgg cgctaacccg gacgtaaact gtattacaaa
    40561 agcggcaaaa agcagagaca aaaaaccccc gctttgcagc gagggttgga aatttggtgg
    40621 agctaagcgg gatcgaaccg ctgacctctt gcatgccatg caagcgctct cccagctgag
    40681 ctatagcccc acgatgcgtt tacgtaccaa atttgctggg tgcaaaattt ggtggagcta
    40741 agcgggatcg aaccgctgac ctcttgcatg ccatgcaagc gctctcccag ctgagctata
    40801 gccccgtcac gtaaagcttg tcgagttgac gggcggcatc atatgaattc cgcccgaatg
    40861 tgtcaacggc aaattgcaac gtgtagtttc aatcgctgaa aaatcaggca aatgaacaat
    40921 tttggaaaac cgctcgcatt cagtagttat tcatgtcacg gtttcctgta aagtggtgtt
    40981 ataaaatgaa ctactaatag acccacatac attcagggaa ttgttatgtc caaggagagg
    41041 atgacaccag atgaacttgc cagactgacc ggctatagcc gccagaccat taataaatgg
    41101 gtacgcaagg aaggctggac aacgtcacca aaacctggcg tccagggtgg taaagcccga
    41161 ctggttcacg tcaatgaaca ggttcgtgaa tatattcgca atgctgaacg tccagaaggt
    41221 cagggagaag cgcctgccct ttctggtgat gcgccgcttg aagtattgct ggtgacactg
    41281 gcgaaagaga tgacgccagt tgaacaaaaa cagtttacat ccttgcttct gcgggaaggg
    41341 attatcggat tgttacaacg cttagggatt cgcgatagca aataatatga aaagattacg
    41401 caataaaatg accaccgaag aactggctga atgcctcggt gtggctaaac aaactgttaa
    41461 ccgttggatc agagaaaaag gctggaaaac ggaaaaattt cccggcgtga aaggcggtcg
    41521 tgccagactt attctggtcg atacgcaagt ttgcgagttt attcagaaca cgccagcctt
    41581 ccataacaca ccaatgttga tggaagctga agagagaatt gctgaatacg ctcctggagc
    41641 acgcgccccg gcgtatcgac aaatcatcaa cgcgatagat aatatgacag acattgagca
    41701 ggaaaaagtc gcgcaatttt tgtcacgtga aggaattcgc aatttcctcg cccgtctcga
    41761 tattgacgaa tcagcataaa taaaaaacgg caggatatta tctcctgccg tttatctttt
    41821 tacacgctaa ttactgctga ttttcgcgtt cagcaataaa atccagcgct ttgttgatac
    41881 gctcgatact gcgggtctta ccaattgcgt gaacggtaac atccagtgct ggagactgcc
    41941 ccgcaccggt tacggcgaca cgcagcggca taccaacttt acccataccc acttccagct
    42001 catccgccgt cgcctgaata gcgtgatgaa cgttttcagc ggtccagtca gtaatcgcgg
    42061 ccagtttgtc acgaaccact tccagcggct gacgcgctac cggacgcaga tgttttttcg
    42121 ccgcatcggc atcgaactca gcaaaatctt cgtagaaata acggcagctc tgtgccatct
    42181 ctttcagcgt cttgcagcgt tcgcccagca gtttcaccag atcagccagc tgcgggccgt
    42241 tacgggtatc gatattttcc tgctcaatgt gccactgtaa gtgagtagca acatactccg
    42301 gcggcagcgc gttaatgtag tgatggttca gccacagcag cttgtcggtg ttgaacgcac
    42361 tggcagattt gctgacggca ttcaaagtga agtatttgat catctcttca cgagtgaaga
    42421 tttcctgatc gccgtgggac cagcccagac gcaccagata gttcagcagt gcttctggca
    42481 aataaccgtc atcacgatac tgcattacgc tgactgcccc gtgacgtttg gacagttttt
    42541 taccgtcatc gccattgatc atagaaacgt gcgcgtaaac cggcaccggc gctttcaggg
    42601 ccttaagaat gttgatctgg cgcggcgtgt tgttgatatg gtcttcgcca cggataacgt
    42661 gggtgatttc catatcccag tcatcgacaa ccacacagaa gttataggtt ggggaaccat
    42721 cggtacggcg gataataaga tcgtccagtt cctggttgct gaactcgatc ggaccacgga
    42781 tctgatcgtc aaaaacaaca gaaccttcct gcgggttagc aaaacgtaca acacacggtt
    42841 catcatcagc atgatgctca tggctgtggc ggcagcgacc gtcataacgc ggcttctcac
    42901 ctttcgccat ttgctcttcg cgcagcgctt ccaggcgctc tttagagcaa tagcatttat
    42961 aagcagtgcc ctcttccagc atctgatcga tcaccgcgtt gtagcgatca aaacgtttgg
    43021 tctggtagta cggaccttca tcccactcca ggctcagcca gttcatgcca tccataatgg
    43081 cttcgatagc ttccggcgtg gaacgctcaa gatcggtgtc ttcaatacgc agcacgaact
    43141 caccgccgtg gttacgtgca aaaagccagg agtaaagagc agtacgcgcg ccgccaacgt
    43201 gcagatagcc tgttgggctt ggcgcgaagc gagttttgat tttcatgaaa tggccttacg
    43261 tttagaaaga tgccgacaac cggcaaatcc tggaaaaatt gagtgggcga tattctatca
    43321 ctcccgcctg attcctcaat gtagttcggg tttctacctt acgcattatt gttttcgttg
    43381 acaaattgcg caacacggtc tgttttgcga tcatcttgct taaatttacg acgaacgaac
    43441 aatttcttta gaaaatgcgt tgactcattt tgaactctcc ctataatgcg actccacaca
    43501 gcgggggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    43561 gatcccgtca tcacccacca actactttat gtagtctccg ccgtgtagca agaaattgag
    43621 aagtgggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    43681 gatcccgtca tcacccacca cttcgggtcg ttagctcagt tggtagagca gttgactttt
    43741 aatcaattgg tcgcaggttc gaatcctgca cgacccacca atgtaaaaaa gcgccctaaa
    43801 ggcgcttttt tactatctgc gatactcaaa gattcgaacc tgcagcaggt ttgagttgag
    43861 cgcagcgaaa caacggagcc gctcgcggcg acggcccgaa gggcgagcga agcgagtcat
    43921 cctgcacgac ccaccaatgc aaaaaagcgc cctaaaggcg cttttttgct atctgcgatt
    43981 tgtaagattg atttttcttc tcaagttcct tgttcttcta ccgatattac ctcgtacagt
    44041 gacgaggact gaacaatggc cactatctct tcaacttcaa ttccttccat acaaacacag
    44101 agtagtaaca gggcatcaca aggtagcgat gttgcttcac aaatcgcgcg gataagtcag
    44161 caaatcatca aactcactca acaaattaaa gagattgtag acactagcgg cagtgctgaa
    44221 gacaagcaaa aacaggccga attgattcaa cagcaaatca ccctactgga aacacaactg
    44281 gcgcagttgc agaaacagca ggcagaaaag gcgcaggaga aagagcagcg tttgtctctg
    44341 aatgtatccc ttctaaatcc tgttgaaaaa acgactcata tcgatatcta tatctgatac
    44401 aacggatccc cttcccgacc agccattagc gcattgccct ggtctcgtac cacgtcccat
    44461 aacgcttcag cggcggtgga aagtgaacgg ttcttcctgc gcaccagcat caactggcgt
    44521 tcaaccaccg gcgtaatgcg cttaaccacc agtgggctac cttcaggcaa tggtagcgcc
    44581 agtgcaggaa gaatactgat gccgatgcct gcggctacca tcgggaacag cgtcgccgga
    44641 tgtccaatct cctgcacaat attcgcctga ataccattgc gcgccagcgc tgcgtcaatc
    44701 agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt tcgctccctg taatgcctgc
    44761 cagggtacat aatcttccac ggccaaagcg ctatcgcgat ggcaaagcag aaagaaaggc
    44821 tcggaaagaa tcgcttcaca ttgcagatcg cccactgggc ctggatcgat gacgatgcca
    44881 aagtcgacat ctccctggcg aatactttcc atcacccact gctgcggacg atcatgcagg
    44941 acaaactgaa tatctggata gcggcgatgg ctttcagcaa tgcattgcgg aataagatgc
    45001 gcggaaatgg tctggctggc agcgacccga acttttccgc tcagttgttg ccccatacgc
    45061 ccggtatcgc gcaacgtgct gttaagttca tccagcagtc gctcaagacg caaagccaac
    45121 tgctgccctg catctgtaag caccacttca cgcgtggttc tgtccagcag gcgaacacca
    45181 gtatgatttt ccagctcctt cacactgtga cttactgccg actggctcaa gccgatacgc
    45241 tctcctgcac gactaaaact tttctcctgc gctactgtga cgaaaacctt taattgtttt
    45301 aaagaataat tcatctgttt tcttcatgaa tggatgcaat aaatcaattt tatttctcaa
    45361 actggaagaa gcacaataga accatcgatc atctggagtc tttatgaaac tttttcgtat
    45421 cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt tctttccggc
    45481 cagaggcgat ttcgtcccct tctttgaaaa tctgaccacc gcagctattg ccctgctgtt
    45541 ctttatgcac ggcgcgaagt tgtcgcgtga ggcgattatt gctggcggtg gtcactggcg
    45601 actgcatttg tgggtaatgt gcagcacctt cgtgctgttt ccgattctgg gtgtactgtt
    45661 tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac tccggttttc tctacttgtg
    45721 cattctcccg gctaccgtgc agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt
    45781 cgcggcggcg gtttgttctg cgtcagcatc cagcctgctg gggattttcc tttcaccatt
    45841 gctggttggt ctggtgatga acgttcacgg tgcaaggggc agccttgagc aggtcggtaa
    45901 aattatgctg caactgctgc tgccgtttgt gttggggcat ctttcccggc cgtggattgg
    45961 tgactgggtg tcgcgcaata aaaaatggat tgcgaaaact gaccagacgt ccattctgtt
    46021 ggtggtttat acagcgttca gcgaagccgt cgttaatggt atctggcata aagttggctg
    46081 gggatcattg ctgtttatcg tggtggtcag ctgcgttctt ctggctatcg tgattgtagt
    46141 taacgtcttt atggcacgcc gactgggctt caataaggca gatgaaatta ctatcgtctt
    46201 ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg gcaaacattc tgttccccac
    46261 atcggtgatc ggtatgatgg tgctgcccct gatgattttc catcagatcc aattgatggt
    46321 ctgtgcggtg ctagcgcgtc gatacaaacg ccagaccgaa cagttacagg cgcagcagga
    46381 aagcagcgcc gataaagctt aaagcggacg cttcaggggc tggaccagtt gcgtcagccc
    46441 ctcggtttta atcagcagcg tgatttgcat tagctcaccg agcttccctg ccgggaactc
    46501 atctttgcgg gcaaaccaca gcagatactc ctccggcagg tcaattaagc gacgcccttt
    46561 gtatttgcca aacggcatta tcgtattggc tatttcaatc agctgctctt tttccatctc
    46621 agctacccag caaacgcagc atttccgctt cgtcgatgac ttcaatgccc agttcctgcg
    46681 ccttcgccag tttagatcct gcagcttcac ccgctatcac cagatcggtt ttcttcgaca
    46741 cgctgcccgc gactttcgcg cccagttcga ccagtcgagc tttagcgtca tcacgcgaca
    46801 tctggcttaa gctgcccgta agcaccacgg ttttaccagc aaacgggctg tcaatctctt
    46861 ccgcgttgat aacgatcggc gcaggccagt gaacaccttc cgccaacagc tcgctgatga
    46921 cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg ggatgcaacg acaatgccaa
    46981 catcaggcac cttttgcagc tcttcaatcg aagcggcttc cagcgcttcc agcgtgccga
    47041 aatatgccgc cagacctgct gcggtggcct cgccgacttc acggatgcca agtgcataga
    47101 ggaagcgagc aaaggtggtt tctttcgctt tttccagcgc gttaaccacg ttttgtgcca
    47161 attttggccc catacgctcc agtccggtca gtttgcctgc ggtgagtttg aacagatctg
    47221 ccggagtatg gacatattct ttttcaacca gctgatcgat gattttgtcg cccattccgt
    47281 caacatccat cgcacggcgg gaaacaaagt gtttcagcga ctctttacgc tgcgcaccgc
    47341 aaatcaggcc accggtacag cgggcaaccg cttcaccttc cacacgctca acgtcagaac
    47401 cacataccgg acaatgcgtc gggaatacaa cctcacgggt atcttccggg cgttcagaaa
    47461 gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg gcgaatcacc actttatcgc
    47521 caatgcgtaa accaagacgt tcgatttcat ccgcattgtg taaggttgcg ttactcacca
    47581 gcacgccagc aacatggaca ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc
    47641 caacctgaaa ctcgacgtca cgcacaaagg tcatctgctc ctgcgccggg aatttaaacg
    47701 ctaccgccca gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt tccagtgagt
    47761 tgaccttaat caccacgccg tcgatatcaa agcccagcgt cgggcggtct tcttccactt
    47821 tgtgatagaa cgccagcact tcttccgccg attcacaaag cgttacccga tcgctgaccg
    47881 gcaaccccca ctttttaaat tgcagtaaac ggccaagatg agtatccggc agctcgcccc
    47941 cttccagaac accaacgcca tagcagaaaa aagtgagcgg tcgcttcgct gtaatacgcg
    48001 gatcaagctg acgcagtgaa ccagctgccg cattacgtgg gttagcaaaa actttcccgc
    48061 ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg aacacttcac
    48121 cacgcacttc cagacgcgcc gggatattct ctccgtgcag cttcagcgga atggcgcgaa
    48181 tagtacgcac attagacgtg atatcttccc cggtggtgcc atcgccacgg gtcgcggcac
    48241 tgactaaaac gccattttca tacagaatac tgacggcaag gccatccagc ttcagctcac
    48301 agcaccaggt gactttctcg ttgtttttca gacggtcctg cacacgtttg ttgaaagcaa
    48361 gaaagctttc ttcatcaaaa acgttatcca gtgacagcat tggtacttca tggcgtatct
    48421 ggctgaaagc tgccagcggc gcagcaccta cacgttgggt aggcgaatca ggcgtaatca
    48481 gttctggatg tttggtttcc agctcgcgca gttcgcgcat cagcctgtcg tattcagcgt
    48541 cgggaatttc cggcgcatcc atcacatgat aaagatattc atgatggcaa agcgtcgttc
    48601 gcagttctgt cagttgttgt tcgattgatt ccatatcgca ccatcaatgc taaaaacccc
    48661 cgacaagcgg gggttcgaag aggagttaat ttgccttaag tgtatcaggc gttggcgtct
    48721 ttgacttcgc ggatgatgtc ctggtactcg cgcaatttct gcggagtcat catactgcgc
    48781 tgatcgtcaa gcacgacacc gcccacttca tcggcaatat gctgcgcaga ttgcagcatc
    48841 agcttgaagt tctgcagctc gtcaccgtaa gacggtacct gcataaagat agtgacaccc
    48901 ggagtagtga aatccttcat ttcaggatca aaggttcccg gtttcaccat attcgccagg
    48961 ctgaataacg ccgggccgct gccatccggg ctaagatgac gatggtaaat attcatatcg
    49021 ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt tagctcgcta
    49081 ccgtgatgcg ccgcgacgtt catgataatc accgcttctt tgcgcttcgg tttatccata
    49141 actggagccg gttccgctac aggctcaggc tgtggtgccg ctacgggttc tgcaggctgg
    49201 aaagcctgtt gtgccggttg cggtgctgaa tgcactggct gcggcgctgg cgcgacctgt
    49261 ggcgaaactg gctgctggac cggttgacgc ggctgcgcag acgcataagg cggttggtac
    49321 tggtgttgcg gcgacggacg agcagcctca tgctcctgag cgttagccgg ggcatgattc
    49381 acgcggtgaa cacgaacctc accaacgccc tcatcatctt cgacatcctc gtcataagaa
    49441 tcgtcgtcac gttttgactt cattcgtttt aatggccgat cgcggaacat agaagatcgt
    49501 tctttacggc tggtccagaa accatgtacc agtaaagcga ttatggcgat cgcgccaaca
    49561 atgattaata tcagacgcaa atcctgcatc attatattct ctgttgttct aacaccttgc
    49621 caccacggca aacatttact cactaagagt atttgccgat tacctcaagt gcaagtgcac
    49681 tattaacttt cacagcacaa agatagatga aatcgtgctt tttgctgttt tttcgaacat
    49741 atcctaactg tccattgcgc aattacccgg taaaatacgc agaattttcc tgggattggt
    49801 caaaaggagc tcatcctgat tatggtttca tcattcacat ctgccccacg cagcggtttt
    49861 tactattttg cgcaaggctg gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt
    49921 ttaccgctgc tggtcaatat tttgttgatg gggggcgcat tctggtggct ctttacacag
    49981 ctcgatgtct ggatcccgac tttcatgagt tacgttccgg actggctaca atggctgagt
    50041 tatctgttgt ggcctctggc tgtcatctct gtgctgttag tgtttggcta tttcttctcc
    50101 acgattgcta actggattgc cgctccgttt aacggtttat tggctgaaca actggaagca
    50161 cgattgactg gcgctacacc gccagatacc gggattttcg gtatcatgaa agatgtgccg
    50221 cgaatcatga aacgcgaatg gcaaaaattt gtctggtatc tgccgcgcgc aattgtatta
    50281 ctaattcttt acttcatccc tggtattggg caaaccgtcg cgccggtact gtggttcctg
    50341 tttagcgcct ggatgttagc catccagtat tgcgattacc ccttcgataa ccacaaagtg
    50401 ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt
    50461 gctttaacca gcctgtttac gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc
    50521 gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg
    50581 taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc
    50641 ccttattcca tcttgcatgt cattatttcc cttctgtata tagatatgct aaatccttac
    50701 ttccgcatat tctctgagcg ggtatgctac ctgttgtatc ccaatttcat acagttaagg
    50761 acaggccatg agtaagattt ttgaagataa ctcgctgact atcggtcaca cgccgctggt
    50821 tcgcctgaat cgcatcggta acggacgcat tctggcgaag gtggaatctc gtaaccccag
    50881 cttcagcgtt aagtgccgta tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt
    50941 gctgaaacca ggcgttgaac tggttgaacc gaccagcggt aataccggga ttgcactggc
    51001 ctatgtagct gccgctcgcg gttacaaact caccctgacc atgccagaaa ccatgagtat
    51061 tgaacgccgc aagctgctga aagcgttagg tgcaaacctg gtgctgacgg aaggtgctaa
    51121 aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc gccagcaatc cagagaaata
    51181 cctgctgctg caacaattca gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg
    51241 tccggagatc tgggaagata ccgacggtca ggttgatgta tttattgctg gcgttgggac
    51301 tggcggtacg ctgactggcg tcagccgcta cattaaaggc accaaaggca agaccgatct
    51361 tatctctgtc gccgttgagc caaccgattc tccagttatc gcccaggtgc tggcaggtga
    51421 agagattaaa cctggcccgc ataaaattca gggtattggc gctggtttta tcccggctaa
    51481 cctcgatctc aagctggtcg ataaagtcat tggcatcacc aatgaagaag ccatttctac
    51541 cgcccgtcgt ctaatggaag aagaaggtat tcttgcaggt atctcttctg gagcagctgt
    51601 tgccgcagcg ttgaaactac aagaagatga aagctttacc aacaagaata ttgtggttat
    51661 tctaccatca tcgggtgagc gttatttaag caccgcattg tttgccgatc tcttcactga
    51721 gaaagaattg caacagtaat gccagcttgt taaaaatgcg taaaaaagca cctttttagg
    51781 tgcttttttg tggcctgctt caaactttcg cccctcctgg cattgattca gcctgtcgga
    51841 actggtattt aaccagacta attattttga tgcgcgaaat taatcgttac aggaaaagcc
    51901 aaagctgaat cgattttatg atttggttca attcttcctt tagcggcata atgtttaatg
    51961 acgtacgaaa cgtcagcggt caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc
    52021 gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga
    52081 ctttagttcc acaacactaa acctataagt tggggaaata caatgttcca gcaagaagtt
    52141 accattaccg ctccgaacgg tctgcacacc cgccctgctg cccagtttgt aaaagaagct
    52201 aagggcttca cttctgaaat tactgtgact tccaacggca aaagcgccag cgcgaaaagc
    52261 ctgtttaaac tgcagactct gggcctgact caaggtaccg ttgtgactat ctccgcagaa
    52321 ggcgaagacg agcagaaagc ggttgaacat ctggttaaac tgatggcgga actcgagtaa
    52381 tttcccgggt tcttttaaaa atcagtcaca agtaaggtag ggttatgatt tcaggcattt
    52441 tagcatcccc gggtatcgct ttcggtaaag ctctgcttct gaaagaagac gaaattgtca
    52501 ttgaccggaa aaaaatttct gccgaccagg ttgctcagga agttgaacgt tttctgagcg
    52561 gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg
    52621 gtgaagaaaa agaagccatc tttgaagggc atattatgct gctcgaagat gaggagctgg
    52681 agcaggaaat catagccctg attaaagata aacacatgac agctgacgca gctgctcatg
    52741 aagttatcga aggtcaggct tctgccctgg aagagctgga tgatgaatac ctgaaagaac
    52801 gtgcggctga cgtacgtgat atcggtaagc gcctgctgcg caacatcctg ggcctgaaga
    52861 ttatcgacct gagcgccatt caggatgaag tcattctggt tgccgctgac ctgacgccgt
    52921 ccgaaaccgc acagctgaac ctgaagaagg tgctgggttt catcaccgac gcgggtggcc
    52981 gtacttccca cacctctatc atggcgcgtt ctctggaact gcctgctatc gtgggtaccg
    53041 gtagcgtcac ctctcaggtg aaaaatgacg actatctgat tctggatgcc gtaaataatc
    53101 aggtttacgt caatccaacc aacgaagtta ttgataaaat gcgcgctgtt caggagcaag
    53161 tggcttctga aaaagcagag cttgctaaac tgaaagatct gccagctatt acgctggacg
    53221 gtcaccaggt agaagtatgc gctaacattg gtacggttcg tgacgttgaa ggtgcagagc
    53281 gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg
    53341 cgctgcccac tgaagaagaa cagtttgctg cttacaaagc agtggctgaa gcgtgtggct
    53401 cgcaggcggt tatcgttcgt accatggaca tcggcggcga caaagagctg ccatacatga
    53461 acttcccgaa agaagagaac ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc
    53521 gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat
    53581 tgcgcattat gttcccgatg atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga
    53641 tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa
    53701 tcggcgtaat ggtggaaaca ccggctgccg caacaattgc acgtcattta gccaaagaag
    53761 ttgatttctt tagtatcggc accaatgatt taacgcagta cactctggca gttgaccgtg
    53821 gtaatgatat gatttcacac ctttaccagc caatgtcacc gtccgtgctg aacttgatca
    53881 agcaagttat tgatgcttct catgctgaag gcaaatggac tggcatgtgt ggtgagcttg
    53941 ctggcgatga acgtgctaca cttctgttgc tggggatggg tctggacgaa ttctctatga
    54001 gcgccatttc tatcccgcgc attaagaaga ttatccgtaa cacgaacttc gaagatgcga
    54061 aggtgttagc agagcaggct cttgctcaac cgacaacgga cgagttaatg acgctggtta
    54121 acaagttcat tgaagaaaaa acaatctgct aatccacgag atgcggccca atttactgct
    54181 taggagaaga tcatgggttt gttcgataaa ctgaaatctc tggtttccga cgacaaaaag
    54241 gataccggaa ctattgagat cattgctccg ctctctggcg agatcgtcaa tatcgaagac
    54301 gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg gtattgctat caaaccaacg
    54361 ggtaacaaaa tggtcgcgcc tgtagacggt accattggta aaatctttga aaccaatcac
    54421 gcgttctcta tcgaatctga tagcggcgtt gaactgttcg tccacttcgg tatcgacacc
    54481 gttgaactga aaggcgaagg cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt
    54541 ggcgatactg tcattgaatt tgatctgccg ctgctggaag agaaagccaa gtctaccctg
    54601 actccggttg ttatctccaa catggacgaa atcaaagaac tgatcaaact gtccggtagc
    54661 gtaaccgtgg gtgaaacccc ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa
    54721 atggcgccca tcggcgccat tttttatgct tccgccagcg gcggcaaaat caattcatcg
    54781 ctctcatgct gctgggtgta gcgcatcact tccagtacgc gcaaccccgc acggtgcacc
    54841 gcatcgttta acgccttccc tttcagcaaa ccactgataa gctgagcaca aaacaggtcg
    54901 ccagtccctt tcaggtcggt ttttacccgt gaatgggaaa tgacattcac gctgtcggca
    54961 ctgaccacca ccacctgcat ctcctgattt tcttcattac cggaggcgct ggtaatcacc
    55021 acccatttta atgtgtctga aagcagactt tttgcggcag caatggcact gtcgagatcg
    55081 cggcaatttt taccggtcag gatttccagc tcaaagatat tgggggtaat tccctgcgcc
    55141 agcggcagta aatattgtcg atacgcctcg ggaaggtcag gtttgacata aattccgcta
    55201 tcaatatcgc caatcaccgg atcgaccatg atcaataggt caggatggtc tttgcgtagc
    55261 gcagtcagcc actcggcaag gattttgatt tgcgatgccg ttcccatata gcccgtggtt
    55321 acagcacgaa gttggcgcag cgcatcacgc tcctgaagcg cacgcaaata gccgctaaac
    55381 cattcgtccg gaatcgcacc accgtagaaa gtgtcataat gcggcgtatt gctcagcaat
    55441 accgtcggca cggcaaagac attcaagccg ttctgtttta tagcaggcac cgcaatgctg
    55501 ttgcctacgc ttccgtacac tacctgcgac tgcacggcga cgatatccgc ctgcagcgcc
    55561 ctactcttat cgttaaacaa caacaaacta ctcatttaat tttttctcct tgccgatgat
    55621 cctcatcgta ttccaaccga aactttacct gattctggca gtcaaatcgg ctattacaaa
    55681 acaaagataa ggtaattcag tgaagaaaat catttgtctg gccattacgc tgctaatgac
    55741 actccccgct tacgcgaagt taactgttca tgaagaagcc cgcatcaacg ccatgctgga
    55801 gggattagca cagaaaaagg atttgatatt tgtgcgcaac ggtgatgaac atacctgcga
    55861 tgaagcggtt tcccacctgc gtctgaagct cggcaatacc cgtaaccgca ttgacactgc
    55921 cgagcagttt attgataagg tagcttcgtc gtcatcgatt accgggaagc cgtatatcgt
    55981 gaagatcccc ggtaagagcg atgagaacgc acagcctttt ttacatgcgt taattgcgca
    56041 gacggataaa acggtgcctg cggaaggaaa ttaatccgct ttggtaaggc atttacagga
    56101 ggtaacatga aaaaatgctt tatttatcac gatgaaaaat cgaataaatt ttggtggata
    56161 gattacgaag gggatagttt agctgtcaac tatggcaagg taggtagtat tggtaaattc
    56221 catacaaaag aattcgataa tgaagaacag tgtctgaaag aagccagtaa attgattgcc
    56281 gcaaaaatga aaaaaggata tcaagaaact ccagacttta acttcatgga tctctactat
    56341 tttgatgatg aagagattgg gttacatctt aaaacgtcac acccaaactt ccagtgccat
    56401 tttactgatc cactttatat gtgttgctgg gatgaagaat ctccttttgg tagcgatgaa
    56461 ggtgctgatg ctctaaacga acttgaaaat agcctccgtt ctggactgtg ctgatttccc
    56521 tcaaatgtta attgaaacta tgtggggtat gaaatacatc gctatggaca gtattcttga
    56581 agaggatgtg cgtgcgcaat tactagccga tgaaatgagc tctatccaga gcaatatgat
    56641 tacctacgcg actgcattcg gtcagatcaa agtcatgggt aaaatctccc ataaacttaa
    56701 aaagatggga ctcaatgcac tagcgcgtca tcagcttacc gcaaaaattc ttcaatgggg
    56761 tgacggtcag gactcaccaa tacttcaaaa aatgattgat gaccttactt cttttccaca
    56821 cgaaaattaa aaactacatt tgtcggcagc aacaactgcc gacaaatcag attattgaca
    56881 aagtgcactt tgtttatgcc ggatgcggcg taaacgcctg atccggcata aaaatattgc
    56941 taattcaata tattgcagaa acattgtagg cctgataagc ggagcgcatc aggcagtttt
    57001 gcgtttgtca tcagtctcca atgctattaa tccttaaatc cctgccccct gactaaaatg
    57061 ctcttcccca aacactccgg tggagaggta gcgatcgcca cgatcgcaga tgatcgctac
    57121 caccaccgca ccagggttag cttttgccac ccgcagtgct ccagcaaccg cgccgcctga
    57181 gctgacgcca cagaatattc cttcccgcac cgccagttcg cgcatggtgt tttccgcatc
    57241 gcgctgatga atatccagca cctcatccac cagcgcagcg ttgaaaatcc ccggcagata
    57301 ttccgcaggc cagcggcgaa tgcccggaat actgctgcct tcttccggtt gcaggccaac
    57361 aatggtcacc ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt
    57421 cgtccccatg ctggagacaa aatgagtgat gcgcccgccg gtttgctgcc agatttccgg
    57481 cccggtggtg gtgtaatgcg cataagggtt atcgggatta ttgaactgat cgagcagctt
    57541 tccttcgcca cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc
    57601 tttggtgaca agaatcagtt ccgcaccata agcatgcatc gccgcacggc gttcctggct
    57661 catgttgtcg ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag
    57721 cgcaatgccg gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc
    57781 gcgcttttcc gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg
    57841 gttattgcct tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa
    57901 cttcaccaga ggcgtattgc ctattgtttg ttctaatgta ctcacgatct ctatccatac
    57961 gtggtgttgc ctgatgcgac gcttgcgcgt cttatcaggc ctacaggtta caaaccttgc
    58021 cattaaaaag cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc
    58081 aggcgctttg tgcgagagca agttcctcat cgcgggtttc aatacgctcg tcgccgttat
    58141 acagccgcgc atgttgcaga ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc
    58201 catgcatcac gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg
    58261 tgtagtgacc tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg
    58321 tacggcggct gatatccact tcccacgggc gcaggaagag atccaccgga ccctgatacg
    58381 caggtgtgta gcccagcggc cagcgatgcg cgccaacatg gaactgcccg ccgcgaatgg
    58441 ttccctgcag gcggttcact tcgcccataa attcgagtac aaaacgggtc gctggttcgc
    58501 gccatacctg attcggcgca tcagcctgtt caatattccc ctgactcatc accactacac
    58561 gatcggctac ttcggtcgcc tcttcctggt cgtgggtcac aaacacgctg gtgaatttca
    58621 gctcttcatg cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg
    58681 cgccaaacgg ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg
    58741 ccacacgctg tttctggccg ccggaaagct gcgccggata acgatccgcc agatgcgcaa
    58801 gctggaccat ttccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgct
    58861 cgcgacgcgg cagcaccgtc aggccaaaag tgatattgtc gaataccgtc atatggcgga
    58921 acagcgcgta atgctggaac acgaaaccga ctttacgatc gcgcgcgtgc aggcgactca
    58981 cgtcggtgcc gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatcc
    59041 gcagcagcgt ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgaccagaag
    59101 gaatatccag tgagatatcg ttcagcacct gggtgcgacc aaaagacttc ttgatattgg
    59161 caatctcaat gctcatgatg ctcctcctgc tgcgcgcgtt tttcctgatt ctccaggcgc
    59221 cactgcaaca tactctttaa aaacagggtg ataatcgcca tcagcgtcaa cagcgcggcg
    59281 gcggtaaagg agccgacggt gttgtagtcc tgctccaaca attcaatctg taacggcagc
    59341 gaaagtgttt cgccgcgaat cgagccggaa accaccgaca cagcaccaaa ctcgccaatc
    59401 gcgcgggcgt tggtcagcac cacgccgtaa agcagcgccc agcgaatatt cggcaatgtg
    59461 acgcgacgga acatctgcca gccggacgcg ccaagcaaaa tcgccgcttc gtcttcctgg
    59521 ctgccctggc ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag
    59581 atggtgacca gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc
    59641 cagccgccga gcgggccgtt ggagccgtag aacagcagat acaccagacc ggcaaccacc
    59701 ggcgatacgg caaacggaat gtccagtagc gtcagcagta actggcgtcc cgggaagtta
    59761 aagcgcgtca ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc
    59821 agcgcgatca tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt
    59881 aaaaccggca tcagcccctt gctgaatgcc tgcacgaaga tgtaaatcat cggcaccagc
    59941 aggatgaacg ccgaaaccag catcccgatg ccaatcagaa accatttgcc ccagttaatc
    60001 gggcgcgcgt cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc
    60061 gaccaaagcg actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga
    60121 tcaccgaagc aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca
    60181 tcagcgacgt cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa
    60241 attcaccaag actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagct
    60301 ccggcagcac cactttgcag aaactctgcc agcgcgttgc accaagcgtt tccgccgctt
    60361 cttcatattc cgggcctaac tcttccagca ccggctgcac ggtacgcacc acaaacggaa
    60421 tgctggtaaa ggccatcgcc accgcaatac caagccaggt ataggtgact ttgatatcaa
    60481 acttcgccag ccattcaccg taaaaaccgt ttacggaaaa gagcgaggcc agcgttaaac
    60541 cggcgactgc cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc
    60601 ccggaaagcg ataacgtgtt aaaatccacg ccatcagcag accgaaaacg ccgttaaaaa
    60661 tcgatgccac aaacgccgac agcagcgtca ctttgtaggc cgcgaccacc tgcgggttgg
    60721 tgatcacctc ccagtactgc gcccagctca tctcagaaag ctgcatcacc agcgcagaga
    60781 gcggcagcag caatatcagg cacacaaaca gcagactggt gccaaggctt aaagtaaagc
    60841 ccggcagcac gcgtcttgaa gaaactgcaa acattactta cgccccgccg ctaacagctt
    60901 gtctaactcg ccgccgctgg tgaagtgggt tttcatcact tccggccatg agccaaattt
    60961 gtcttccacg cggaacagtt cggtctgcgg gaatttatct ttcagtttgt ccatgacttc
    61021 cgggttattc acacggtagt aatagtcggt gatgatggtc tgcgcctgtg ggctgtagag
    61081 ccagttcagg taggcttttg cggctttttc cgtaccgttg gcctgcacat ttttatcgac
    61141 ccacgccacc gggaattccg ccagaatatt ggttttcggg atcaccactt caaagccctg
    61201 tgcttcatac tgtttacgga tgttgttcac ttccgattca aagctaatca gcacatcgcc
    61261 caagccacgc tcggcaaaag tggtggttgc gccacgaccg ccggtatcga acacttcaac
    61321 gtttttcagg aactgggtca taaactgttc ggttttggct tcatcgccac cgtcagcttt
    61381 atccgctgcg ccccatgccg ccagataggt ataacgcgcg ttacccgacg ttttcgggtt
    61441 cgggaaaatc agcttcacgt cggagcgcac caggtcgttc cagtcgtgga tattcttcgg
    61501 gttgccctta cgcaccagga agcccatggt ggagtagaac ggcgagctat tattcggcag
    61561 gcgcgactgc cagtcagccg ggatcagctt gcctttgtcg tgcaggattt gtacgtcggt
    61621 cacctggtta taagtgacaa cgtctgcttt taagccctgc aaaatcgcca gcgcctgttt
    61681 tgatgacccg gcatgagatt gttttatcgt cagtttgtcg ccgccgttat cttttgccca
    61741 ttgctgttca aacggcggat tcagggcggc aaacagctcg cgggagacgt cataagaact
    61801 gttcagcagt tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag
    61861 tgagttcttt ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct
    61921 aatagccatc atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg
    61981 atttggaagt taaaaaggga ataagaactt ggttcaacag gcgtaattgt tgcaggcagt
    62041 ttggacacgg acagcgcgga gaaaccggag cgtacacata gtacgtgagg atttcgagca
    62101 ctgcccgggg ccaaaatgac aaatgaaata gcctggtgaa cttagttcaa gacgaaatcc
    62161 tccccacaca tgcagggagg aaacagaggt gaatcagata ccgacgctaa ccgtctccgg
    62221 cagtgtgctg ccgccatcaa tcacattctg tgtaccggtt aaatagctgg attcatccga
    62281 tgcgaggaag gccgccagtt cgccgacttc cagcggatcg gcgaggcgac acagcgggat
    62341 tgcttttgcc atttcagtca gcaccgattc tggatcttcc gggttcgact ggcgggcaat
    62401 gctttccgcc attggcgtgc ggacgtatcc cgggcagatg gcgttaacgc gaataccgga
    62461 ttgcgcgtac tccaccgcca gcgatttagt caggccaaca atcgccgctt tcgttaaggc
    62521 atacgccgtt tcgccaggat cggccaccat atcaccagtg actgaagaca tcatcacaat
    62581 gcgaccatct ttgcgcgcaa tcatctccgg cagcaccgcc ttcgtgacgt tccatacgcc
    62641 tttaatattg atatcaatat ggaaatcgcg atcttcatcg ctcatatcga ggaaactgcc
    62701 cagacgacaa acgcctgcgt tattcactag gatatcaatg cgcccttctt tttccttcgc
    62761 gcgtttgata gctgcggcta ccgacgccgg gtcacgcaca tcggcgacaa ccgccgtaca
    62821 gcgatgacca cgaccacaca gttcgtccgc tagcttttcg atctcagggg agatatccag
    62881 caagattagg ttcgcgccat gacgtgcaaa agttctggca attccttcgc caattccctg
    62941 caatgcgccc gtaatcagtg ctgtcttgcc cgtgagttta cccattttaa tgctctcctt
    63001 gttaatacag agcgttacac tccattccct ctgaaaatac aggataaaca aaatattgct
    63061 gcggatcagc tcactaattc aggaaaatct caatccagtc gctgctttat gcatcatttc
    63121 aggcgttgtg ttagctcact gctgcgctga atcattttca atgacgccac atcattgatg
    63181 ttgcacaaga ccaacaaaca gtaaatcggt aacagaattt tgcgccgtgc gggtagacat
    63241 cgacgagcta cgccattcgg tttcaccgga aacggtatcg agcgtgaaat gcgccagccg
    63301 ccgcagcgga gagtcagcca gagaagtaat ggcaatcacc gttgccccct gctttcgtgc
    63361 cgcttctgcg cacaagacaa tctctttttt actgccgctg taagagatag cgatttgtac
    63421 atcgcctttt ttcaacgcct gggaaacggt ggcttgtacg tgcgtgtcgg cttcgcaggc
    63481 cacgcgatag ccaattttca tcagtttgaa cgacagatcg cgccccacca gcgccgatcc
    63541 gcccaggccc gttatctgga taaacggtgc tttactgatg acctcaatga ttttttgcag
    63601 ccgcgcgtag tcgaacaacg cacaggtttg ctccagcgcc agctcttttt cgcgattcag
    63661 tttgcgggca atcacttcca gcgaatcatc actggtaatc gaactgtgca ggtgcagcgc
    63721 cgtggcattc gttttctccc ggctggcact gtattcgcca attaacgcca tacgcaattc
    63781 agtaaatcct tgcgcgccga gcttctgggc aaatttcaca atgctcgact ggctgatacc
    63841 gagctgtttc gccatctgcc gtgaagagac ggatttcagc tcgctcactc tggcccgcag
    63901 aaagtcagca attttttgct cgttcccggt aaattcactt tccgcattgc gtattttcgt
    63961 caggtacaac atcttccact ctcctgggct ggcagcaaaa attccgctta agttcttatg
    64021 acgctcttca cactctgcga gtgtagactc aatgattcct ttaagtctgt caattcggga
    64081 tatatgattc catacatctg caattatttg atcgtaaata gtaaggtcac caccgatgca
    64141 actagaaaag atgattactg aaggctcgaa caccgcctcg gctgaaattg accgcgtatc
    64201 gacgctggaa atgtgccgga ttatcaacga tgaagataaa accgtaccgc ttgccgttga
    64261 tcgcgtcctg ccggatatcg ccgcggcgat cgatgttatc cacgcccagg tcagcggcgg
    64321 cggtcgtctg atttatctcg gtgcgggaac atccggtcgt ctggggattc tggatgccag
    64381 cgaatgtccg cccacctacg gcgtgaaacc gggtctggtg gttggtttga ttgctggagg
    64441 cgaatatgcc attcagcacg cggtggaagg cgctgaagat agccgggaag gtggtgttaa
    64501 cgatctgaaa aatattaatt taacggcaca ggatgtggtg gttggcattg ctgccagcgg
    64561 tcgcacgccg tatgtgattg ccggactgga atacgcacgc cagctcggct gccgcacagt
    64621 gggaatttcc tgtaatccgg ggagcgccgt ttcaaccacc gctgagtttg ccattacacc
    64681 gattgtaggt gccgaagttg ttaccggttc ttcgcggatg aaagcaggca cagcgcagaa
    64741 actggtgctc aatatgcttt ccaccgggct gatgattaaa tccggcaaag tgttcggcaa
    64801 cctgatggtc gatgtggtcg ccaccaacga aaaactgcat gtgcgccagg tcaatatcgt
    64861 taaaaacgcc accggatgta atgccgaaca agcggaagcg gcgttaattg cctgcgagcg
    64921 caactgtaaa acggccattg tgatggtgct gaaaaatctc gatgccgcag aagctaaaaa
    64981 acgcctggat caacacggcg gctttattcg tcaggtttta gacaaggaat aacccatggc
    65041 caaagagatc agcagtgaac ttctgaacac cattcttacc cgtgtcggcg gaccgggaaa
    65101 tatcgccagt tgtggtaact gtatgacgcg cctgcgtctg ggtgtacatg acagttcact
    65161 ggttgatccc aatatcaaaa cgctggaagg cgtgaagggc gtcattttga ccagcgacca
    65221 ggtgcaggtc gtttttgggc ctggtaaagc gcatcgtgcc gcaaaagcga tgagcgagct
    65281 gctgggcgag gcaccggtac aggatgccgc agaaatcgcc gcccagaaca aacgtcagtt
    65341 aaaagccaaa caaacctccg gcgtgcaaca atttctcgcc aaattcgcca ccatcttcac
    65401 gccgctgatc cccggtttta ttgccgccgg tctgctgctg gggatagcga cgttaattgc
    65461 cacggtgatg cacgttccgg cagatgctca gggcacgcta cccgacgcgc tgaattttat
    65521 gaaggtgttc agcaaaggtt tgttcacctt cctagtgatt ctggtgggct ataacgccgc
    65581 ccaggcattt ggcggcacgg gcgtcaatgg cgcaattatt gccgcactct ttttgctcgg
    65641 ttataacccc gctgcaacca ccggttacta cgccggtttt cacgatttct ttggtctgcc
    65701 catcgatccg cgcggcaata ttatcggcgt gctgattgcc gcgagggcct gcgcccgcat
    65761 tgaaggcatg gtgcgccgct ttatgccgga cgatctcgac atgctgctga cctcgttaat
    65821 caccctgctg atcaccgcta cgcttgcgta cctgatcatt atgccgctgg gtggctggtt
    65881 attcgaaggt atgtcatggc tgtttatgca cctgaacagt aatccgttcg gttgtgcggt
    65941 tttagccggg ctgttcctga tcgccgtggt atttggcgtg catcagggct ttattcctgt
    66001 ttacctcgcg ttaatggaca gccagggatt caacagctta tttcccatcc tttcaatggc
    66061 aggcgcgggc caggtgggcg cggcgctggc actctactgg cgggcgcaac cgcacagtgc
    66121 gctacgcagt caggtacgcg gggcgattat tcccggcctg ctgggcgttg gtgaaccgct
    66181 gatttacggt gtcaccctgc cccgcatgaa gccgtttgtt accgcctgtt taggcggcgc
    66241 ggcgggcggt ttgtttatcg gattgatagc ctggtggggt ctgccgatgg gcttaaacag
    66301 cgcctttggg ccgtctggtc tggtggcgct gccgctgatg acttccgcac aaggcatcct
    66361 tccggcaatg gcggtttatg ctggcgggat tctggtggca tgggtttgcg ggtttatttt
    66421 caccacgctc tttggctgcc gtaacgtcaa tctggactga tattatgaaa cggacaatgc
    66481 tctatctttc tctgctggct gttagttgta gcgtcagcgc cgcaaaatac cctgttctga
    66541 cagaaggtag ttgatcaagt atatgcggcg ttaaagcagt aataaaaagg ccagtcagta
    66601 atgttactgc ctggccttta atcaatgaat cagaaacgct tacagcacca tcaacttgtc
    66661 cagcgacggt gcgaaataat agccgccggt taccggtttg gtgaaacgca acatcgcatc
    66721 acgtttgcca tcggtatcgc caaacatgct cagcagttgc tgctcaatgt tatgcagacg
    66781 cgcgcagtag gcgcagaagt acagaccgtg agtgccactg gcggtgccgt acggcaggct
    66841 ctggcgaaca atcttcagcc ctttgccatc ttctttcaga tcaacgcggg tgaggtgaga
    66901 ggtttccgga cgttcgtcgc cgtcaatctc ttcgttggcc tctttggtgc gcccgatcat
    66961 catctcctga tcgtgaacgc tcatccggtt gagctgcttc aggttgtgct cccaacgctg
    67021 cacaaacaca tagctgccgc cggcatccac gccgtctttg ataaccgcca cttcgcggcg
    67081 cgtctcttca cccgccgggt tttctgtacc gtcaacaaag ccgctcaggt cacgctcttc
    67141 cacccaacgg aagccgtgga tctcttcttt cacttcaatg cagtcaccaa aggcttccat
    67201 cgccgcctga gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac
    67261 atcgaactgg gtcgtcggcg caaggccttt accgtagccc ggaaaatctt tcagctcttc
    67321 tgccccaacg ccgccgctca gagcgcgcca ggtgttgtta ccaaaggcaa ccaccgcacc
    67381 aagatgcgcg tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa atgttttact
    67441 ggccgcacgc agggcgtcaa cttccccttt cacgttggct tcgatccaaa tcgccgcgcg
    67501 gcaatgttct ggcaaaatac cactctgaac ctgagacatt gttcctcctg aaaataagaa
    67561 tgccacgcaa tccgtggcat tgttgagcgc tattttacgt gttttttcgc gacggcggtt
    67621 tgttcagacg caaattaacg ccgccagata attttactga ctttccagtt tttcagggta
    67681 tcgtccgaag gcattaaccc ttcaggacca cgccattccc cgctgaactg gtagctaata
    67741 tgttgactgc cttcggcttt acattcgacg gcacgattat catcaccatc agctttttgg
    67801 caattgccaa atgctttgct gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg
    67861 tcagcaggaa tatcgctatc cagcacgtca atgcgactga tcgtgccttg atcgccgtta
    67921 atcaccatcg ccacgttgtc gcctttcatc acttcaaaaa agcgcaccac gttgccgttc
    67981 gcggttttca ttccgctgcg caggcgataa tcgccatcaa gcgcatcagc aatggcttgt
    68041 tcttgcagtg gtgtggacgc cgttaattca cccacgccct gctcgctcac tttggtggac
    68101 gatccaaacc agttccacgg gttagcggca gaccagttaa ctgacgacat cgtggaacag
    68161 ccggtcagca tcaacggcat agcgcataac attaaacgca gcgatttcat gtcacttcct
    68221 ttggttattc aataacgttg cttggagtgc aaattcacca aaaagtgccg ttattctttt
    68281 tcttcattaa aacaagcgcg taaacgtcga ttggtcagca gccagattaa cgccacaata
    68341 tccgccacta ccagcgccag accgataccg ctaacggatt caccgttcag ccacagccac
    68401 ggttgccagc aaagtaaaac cacctgcgcc aacagcaaca gaaaatagag cacatgccag
    68461 gtgcgaggaa acgaagcccg ccgaccgctc aacaaaaacg ccagcaccgc cggaataccg
    68521 ggaatcagcc ccagccagaa attatcgtga tcgggataaa acagatttag cagcgcagta
    68581 ccctgctcgc gcgacgcacc ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat
    68641 agcacaagcc agaagagcaa gggtaaacgc aggcgtccgt gcgcatcata atggacagga
    68701 tggaactcag tactcttcat cttcaatcaa acgcttaccc agactcagca cgtcggcgtg
    68761 ttcatatccc aggcgttcat acattccgag caccatgtcg ttatcttccg gcacgttgat
    68821 ctgaattttc gggcagccgc gagcaatcag ctttttctcc agccggttaa gcaacgcatt
    68881 ggcaattcca cgcccacgaa actctggatg cacgccaaga taataagcag acccgcgatg
    68941 cccgtcataa ccgcccatca ccgttccgac cacttcaccg tttacctcag cgaccaaaaa
    69001 caaactgacg tcatggttca tcttacgctc gatgtccatt tccggatcgt tccacggacg
    69061 cagcaagtcg caacgctccc aaagggtgat gacctcttcg aaatcttcct ggcgaaatac
    69121 gcgtatctcc atggtattcg ttaccttttt gcgggttaaa aggctgatta tggcgtgaac
    69181 ggtcgaatta gccaatatct gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt
    69241 caactattga aatgaaaagt aaaacaattc tcaacagcaa accgtcgtaa cggattacgc
    69301 gatacgatat aacatctgga actttattat tacaactcag gccgtatgag cacttttaaa
    69361 ccactaaaaa cactcacttc gcgccgccag gtgctgaaag ccggtttagc tgccctgacg
    69421 ttgtcaggaa tgtcgcaagc cattgccaaa gacgaacctt taaaaaccag caacggacac
    69481 agcaagccga aagcaaaaaa atctggcggc aaacgtgtcg ttgttctcga tccaggccac
    69541 ggcggaattg ataccggagc gatcggacgc aacggttcga aagaaaaaca tgtggtgctg
    69601 gcgattgcta aaaacgtccg ttccattttg cgtaatcatg ggattgatgc gcgtttaacg
    69661 cgttctggcg atacgtttat cccactttac gatcgcgttg aaatcgccca taaacatggc
    69721 gcagatctgt ttatgtcaat tcatgccgat ggctttacca acccgaaagc tgccggtgct
    69781 tcggtatttg ccctctctaa ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa
    69841 cgcgaaaacc gcgccgatga agttgccggt aaaaaggcga ctgacaagga tcacctattg
    69901 cagcaagtac tgtttgatct ggtgcaaacc gatactatta aaaacagcct gacgcttggc
    69961 tcgcatattc tgaaaaagat taagccggtg cataaactgc acagccgcaa caccgaacaa
    70021 gccgcgtttg tggtgttgaa atcgccgtcg gttccttcgg tgctggtgga aacctcgttt
    70081 atcaccaacc cggaagaaga gcggctgtta ggcacggcgg cgtttcgtca gaaaatcgcc
    70141 acagcgattg ctgaaggcgt gatcagttat ttccactggt tcgacaacca gaaagcacat
    70201 tcgaaaaagc gataagttat gaaacccgac gcacaccagg ttaaacagtt tctgctcaac
    70261 cttcaggata cgatttgtca gcagctgacc gccgtcgatg gcgcagaatt tgtcgaagat
    70321 agttggcagc gcgaagctgg cggcggcggg cgcagtcggg tgttgcgtaa tggtggtgtt
    70381 ttcgaacagg cgggcgtcaa cttttctcac gtccacggtg aggcgatgcc tgcttccgcc
    70441 accgctcatc gcccggaact tgccgggcgc agtttcgagg cgatgggcgt ttcactggta
    70501 gtgcatccgc ataacccgta tgttcccacc agccacgcga atgtgcgttt ttttattgcc
    70561 gaaaaaccgg gtgcagagcc cgtttggtgg tttggcggcg gcttcgattt aacccctttc
    70621 tatggttttg aagaagacgc cattcactgg caccgcaccg cccgtgacct gtgccagcca
    70681 tttggtgaag acgtttatcc ccgttacaaa aagtggtgcg acgattactt ctacctcaaa
    70741 catcgcaacg aacagcgcgg cattggcggg ctgttctttg atgatctgaa cacgccagat
    70801 ttcgaccact gttttgcctt tatgcaggcg gtaggcaaag gctacaccga cgcttattta
    70861 ccaattgtag agcgacgtaa agcgatggcc tacggcgagc gcgagcgcaa ttttcagctc
    70921 taccgtcgcg gtcgttatgt cgagttcaat ctggtctggg atcgcggcac gctgtttggc
    70981 ctgcaaactg gcgggcgcac cgagtcgatc ctgatgtcaa tgccgccact ggtacgctgg
    71041 gagtatgatt atcagccaaa agatggcagc ccagaagcgg cgttaagtga gtttattaag
    71101 gtcagggatt gggtgtaact ccctcccccc cattcccgca tccgctgatg caacgtcaac
    71161 gacggcttct cggcaaacag ctgctggtaa tccgtggcaa attgccccag atgccagaat
    71221 ccccactgca tggcggcgtc ttttaccgtt gtgctttgcg accacgggct tatcagttcg
    71281 cggcgtacgg cgttcaggcg aatgcgtttc agccacgcgt ttgggccaat gcctaaaata
    71341 gcgtgaaacg cgttttgtag cgtgcggcga ctgacatgca gttgattaca caaatccagc
    71401 accgtcaccg gttcggacat gttttccagc acatattcac gggcgcggga aagcaaccga
    71461 cggtaactct gatgactgat gctttccgcc gtcaccattg gttgcgcttc ttccagcatc
    71521 gcccccatcg ccattagcaa attatccccc agcacttttc gcactgcagg ctgatggaga
    71581 ttttccgggt tctcgcaaaa cgtcgccagc gcctgttgga caaagcccca cagcgcggct
    71641 ttatgctgct ctttcacttc cagcgccgac tggctacgca gcatatgtaa tacccgatcc
    71701 ggattgtgca aaaagttagc ctgacaggtg atgacatctt cagacagcac tacgcccagg
    71761 atcgtataat cgtccggcgt acttaattca aactcggttc caccggggcg cgttgctatt
    71821 tctgcgcttc ccagacattg cgaaccgata aatccctgct caccgcgcgt cgccggaatg
    71881 ccaaaccaga acgagttcgg ccagaccagg cacgactgac gcagcgccag accggtgtat
    71941 tcacgaaaaa cctgaatatc atcgagtaga atttccgtaa attcaccatg aaacttgccc
    72001 ggatgcagct gatcgtaaat ctgctgccag gcggtaatcg tcaaagcatg ttcatagaca
    72061 tccgttgtct gtcgttgatg aacattatcc acttcgacct tcggcgtgag cttcaggttt
    72121 tcgggtaagg gttcatgata aagatggtgc aaattggctg tacgggtctt tttcatgatg
    72181 ttaatgccgg gtgttgtagg acacccggca cctccgacag gttaatgggg cttgagacga
    72241 taacgactac tgcgtttacg ttacgtcccg gcagaaaaga gctgttcgag cgcatttctg
    72301 gctttttcca gcggccagcc aaagtgggcg gccacttctc ctgccgtcat tccctgacat
    72361 actgatgtta acagcgccaa caactcgtca gcagattccg cgacgataac tggcgcgtcg
    72421 ggttccttta cgggttgctt cggctggcgg ttaaagccag taaccagcca ctgggtgtca
    72481 tcgtctggcc gaccgatctc cttacggctg atgacacgcc cggtacgcat cgcggcagca
    72541 caacctgcgt ccagcgccgc acgacacgcc gccagatcgc cttccaccac cagcgttaag
    72601 cgaccagggt caagcacttc gtgactgagc agacgaacgt tagctgcttt gagcatggca
    72661 tccgctgcat ctatcgcggc gaccatgccg tccacttcca gcaatcccag tgcattgatc
    72721 atcggcaacc tccgttacgc acgctggatt ggattacgcg cgatttccag cactgcatcg
    72781 gtaaaggcgt tacaggctgc tttacacgcg gcctggctac cggttaaaaa tgctgccgag
    72841 tagttggttt cagacggtgg cgggacgtag gtcaccagct gtacgtcggc agatttcaac
    72901 gcggcatcaa tgccgtaggt cgcttccagc ggcggtgcca ccagatacgc catcggatcg
    72961 ccaagcgtga tcccggcggt tgatgaaaga taagaacctg tacgcgaaac tacatgcgcc
    73021 aggaaggcgg tgttttctgc gtcattggcc cactggaaag ctgcgccatt ttcaatatgt
    73081 gcaaccatcg catccagacc ggcacgcact tccgccgggt ttggcccacc aagcataatc
    73141 agcacttcac cggcagtcgg tgacgggccg tgtgccgctc ccgcatacag cgagcgacca
    73201 tataccactt cgaccatcgc ttgtttggtc gcttcgtcag ccgcaatgta ggttacgtcg
    73261 tcagaatcgg cagaaatcag cccgaggcta cgaatatgcg gcggcaattt tagttcacgc
    73321 gcaaattcag cgttaacgga ggcaatcacc cgcatggcgg ttaccgacgg tcgaatcaaa
    73381 tctaaagctg gcatgatgcc tccttatcgg gtcatgttga tgccggatgc tttctgctcc
    73441 agcatacgtt tcgccaaatc cacaatgacg gctgcggctt caaccggcgg cgtgccgccc
    73501 tggtgaatgt tagagataca ggtgcgatcg gcctcaacgg tggtcgccat acgcggcgag
    73561 taaacggcgt agcaggagag gctttctgac tgccctaacc ccggacgttc ccccaccagc
    73621 aggatcacca ctttcgcgcc gaggatctca ccgatctgat cttcaatctt cacgcgacca
    73681 taacgaacaa agaatggcgt gccgactttc agcccggcct gtttcaggcc cgccatcagc
    73741 ggcggcagga tctcttcgta gttgacggtg atggcatcag ttgacaggcc atcagaaata
    73801 acgacctgta cgtccggatt agcaacacac tgtgctttca gcgcttcaac agcttctgca
    73861 cacaggcggc ggcccatatc cgggcgagtc aggtagagat ttttgtcgct aatttcagag
    73921 cgtacttcca gcaagccctg cgctttcacc cactcttccg gcacttcttt cagtacggta
    73981 tctttcgaac gagagtgatc ggccaagaaa cgcagcagcg cctgagtacg tggacgcgga
    74041 ccggcacgac cggtacaaac gcgggccacg gtgctgcggc gcagttctgt taatacgtct
    74101 gcgcgatgcg ggttttcaac accaatccac gcttttgctt cagcggaacc taaatccagc
    74161 gcacagcttt ccgaggtcac cggtgccgca cagttggtgg tggcgcactt tgcttctgac
    74221 ggcgcggggg ccgcttgtcc cattgacgcc atcacgctgc gtacaatttc ttcaatctgt
    74281 ttttgatcca tgatgtgtta tctccgcgtc atcagaagaa cagtgacgga tcgcccgccc
    74341 gtttggtcag gcgaccgttt gccataatgc ccatgctttc cagccagcgt tcaaactctg
    74401 gtgacgggcg caggttgagt aactgacgca cagtggcggt atcgtggaat gcggtggtct
    74461 ggtagttgag catgatgtca tcacccagcg gcatccccat gatgtagttg cagcctgcgg
    74521 tggcgagcag gatcatcagg ttttcgttga ggttctggtc agagtcagcg tggttggtgt
    74581 agcagccgtc acagcccata gagatgccgc tcagcttgcc cataaagtga tcttctaagc
    74641 cagcacggat aatctggcgg tcgttgtaga gatactccgg cccaataaag ccaaccacgg
    74701 tgttgacgat aaacggatcg taatgacgcg ccagcccata gttacgtgct tccatcgtca
    74761 cctggtcagc gccgaagtta gcgccagcgg atagcgcaga gccttgtccg gtttcgaagt
    74821 agaggcagtt ttccccggcg atacgattga actctgcgcc cactgcgcgc gcttcgtcga
    74881 gcatcgccag ttccacgcca aactctttca gccctttttc gctgccacag atactctgga
    74941 aaatcagtcc gccaggagcg ccgcgacgaa tcgcttcgat ctgggtggtg acgtgcgcca
    75001 gtacgcagcc ctgagttggg atgttgaatt tgtcgatcac gccataaatg gtatccagca
    75061 cgcggcttaa gttttccacg tcgtcagtta ccgggttaac gccgatcacc gcatcgccca
    75121 ccccgaagga aagcccttca tagatttgcg cagcgatact ttgcacgtcg tcacgggtat
    75181 cgttcggctg caaacgggcg ctaaaggtgc ccggaatacc gatggtggta ttggcctttt
    75241 tgattaccgg cattttcttc gcgccgtaga tcaggtccgc gttggagcaa atcttcgcta
    75301 tcgccgcgac cacttccgag gtcagccctt tgcgggtaaa ggcaatgtcg tccacgctgg
    75361 tttcatcgct cagcacatac tcacgcagtt cgctgatgct ccagttttta atctggttgt
    75421 aggccgtttc gttaacgtcg tcctgaatca gccgcgtcac gcagtcatct tcataggcaa
    75481 tcaccggatt attgcggatg tccgctacgg tcatttccga caacacctgc tttgccgcca
    75541 cgcgctcctg tgagcttgcc gctgcaacgc ccgccagcac atcccccgaa cgcagttcgt
    75601 tggctttagc cagcacctct tttacatcct taaactgata tacattgccg aacaatgtgg
    75661 tctttagttt cataagtcgt tccctcagga agaagtgatg agtaacattt ctcacagcct
    75721 gcaaacaatt ctatttcatc gttagccctt gtcatgtaag gtgatttcct taacacgccc
    75781 gttacttatc ctgccgaacg caaccgaact cgccggatac atgagcgggg ttatcgagtc
    75841 gttagatcga ggtatccggt tttgagcaaa acaccactca cagcaaaggc catagacgcc
    75901 gcacaaccaa aggacaagca atacaaactc accgcttcac tgacgcccgg cctgtttttg
    75961 ctggtgcatc ccaacggcag caaatactgg cgattccggt actggataga caagaaagag
    76021 cggttacagg ctattggggt atatccgctg attagcctca aggaagcccg caaacgcgcc
    76081 acagagagcc gtttactgat agcccaggga attgacccaa tggatttggc acgcaaggag
    76141 aaagccattg atgcactcaa catggcggca ggttttaaga cggtagcgga ggactggctg
    76201 gctaccaggg taggcggctg gagtgagagc tatacaaagc aggtcaaatc agcactggag
    76261 aaagacgttt atccggtact tggcaagcgt tcaatcgttg atataaccgc ccgtgacgtg
    76321 ctggcattac tccagaagaa agaacgtacc gcaccggagc aggcccgcaa gctacgccag
    76381 cgcatcggtg agatcttcaa gtttgccgtt atcaccgaac tggtaacgag gaatccggtt
    76441 gcagatcttg atacggcact gaaggcccgc cgacctggtc acaacgcgtg gataccgatt
    76501 agtgaaattc cggccttcta caaagccctt gagagggccg ggagcgtcca gattcaggcg
    76561 gcaatacgtt tgcttatatt aacggcatta aggaccgcag aactgcgttt gtgccgttgg
    76621 gaatggataa acctggagga cgcaacaatc acattgcccg ctgaagtcat gaaggcccgc
    76681 cgcccgcatg tggtcccgct atcacggcag gcggtcgagc tattgcagga ccagttcaca
    76741 cgcagcggat acagtgtttt cgtgttcccg ggccgattca tggataagcc attatcagcc
    76801 agcgcgatac tcaaggcgct tgagcgtatc ggatacaagt caatagccac aggccacggc
    76861 tggcgcacaa cgttcagcac cgcacttaac gaaagcggca ggtataaccc tgattggatt
    76921 gagatccaac tagcccacgt cccgaaggga attaggggag tttacaacca agcggcctat
    76981 ctcaaaatgc gccgtcaaat gcttcaggac tacgccgacg cgattgattc gatactggct
    77041 gatggtatca atccgcttga actgtaatga atgattaatc acgttttccg acgactccac
    77101 cggagtaggc cggataaaca ctggacacct tcaaaataac tattcacttt ttccggattc
    77161 gtccggcttt ttttgtcttt gccgatggga tcaattattc caattattcc agtatcgttg
    77221 gaataaatga ataaatatca atgacttaaa gccgtgtttg caacagggtg gatcggggat
    77281 cgaaaatagg atcaacacaa aaacaccctg gaggctacag cacactaggg ggacacaaaa
    77341 acaacacagg aaaaaaatac catgtaccaa tcagcaaccg cctacgtggc gaaaccaggc
    77401 gaaacactgg tcaaacacaa aattgcaaca ctccaagcac ttcaaacact ccaagccctt
    77461 ccggaacagg ttcaggccgc ctttcgtgat ggcatgaaca tcatccgctt cgatgggtat
    77521 cgctgggaat acaagccagc aactggattc gtgcgcatag atcaaatctt gtcgccagcc
    77581 aatgaagagc cagcgctagc caatgcagaa ccagcgcagg agcaggagca ggagcaggag
    77641 caggagcagg aacaggaaca ggaacaggaa caggaacagg aacaggaaca ggaacaggaa
    77701 caggaacaga ataaagcggc ggctattgcc gagttcaagg agctgcaaag acaacaaatc
    77761 acagcgcagc aagaagaatg ccgccacccg ctggcaatgc ccaagccagt agcgaaagcc
    77821 aacacagaca agccgacttc atggtttaac gaagaagcgg aagaagattt gcgttttgat
    77881 agtaaagagg agcagttgcc tgaagagcgg gaacaggtgg aaacggaaga ccagcaaaac
    77941 gattatcgca accttccagc cgtcaataac gctatggctt acctcttcaa aagcgggctg
    78001 tttcacaagc tgaaaggcga tgagttaaag ctatatacgt ggatggtatc caagtgcagg
    78061 tatcgcggta acgacaaata tgagatttgg tattcccgca ccaaaatggc aaaagcgtta
    78121 ggtaagagcg ttgacacgat agatcgccac ctggaagcgt tgagaaagct gggacttatc
    78181 caggaaatgc gccaggcact gaggggcaaa tgtggaaaaa cctacagact gcgaatcccg
    78241 tcaaaaggca cagcagaaga ggcctcaatg acggcacagc agcacgaaag agaagcaaaa
    78301 gtcagctacc tggtacacta ggcagcgggt cagcaaaggg gggcaaatgc cccttttttc
    78361 atgtcagata atcagatcat tacctcaata aactaataat cacatcgcgg ttacaaccag
    78421 tttgatagtg atagctcaaa cccagacgtg gcgctggctg caagcaaaat aagccgcaaa
    78481 aatgcggtcc tatttgccag aaccttttat tattcaattg gttagtgcaa gaaaaagcgt
    78541 gattactgtt caagaagcat ccagaaaggt catgattttg ccatgatttt gacgctgaaa
    78601 aaaaataggt gggcattttt gcccactcac tagccgcaaa tttgcccact cactagccgc
    78661 atttttgcgg cccaaagtaa taaatacaga gaaataataa atacagatca gcgccaagat
    78721 ttttcgtctt ttcggcggaa tcatgagact agtgcttacg tcctgataac ctccagagca
    78781 gaccttaact tctgtgcagc aggactacgc tcattgccgc tcggctaggt caaatcctca
    78841 cgataaagct gttcggattg tgaccataat gttaaaggac tctttaaatc attccttgac
    78901 gaattaacta gctggcttaa gcggaatagc atgcaggata aaaacaaaac cagcttagca
    78961 gtggggagag cttcgcttcg ctcgtctcct ggggccaatg ctcaatgaaa cgacgtaagg
    79021 cacggagcgc tcaccatagg tagacacata aacatctgag agcgcacctt ctatcgcgct
    79081 acgcgctcaa taaaaaccaa cgacaccgcc acactgcaag accaatggtt tttatcgcgt
    79141 atacagacca gacaggaaac agcagcaaag cagcgtatag ccaggtgcag acagcacact
    79201 cacacgacgc acagcaggct taagtgatca ctcaggaaac tttatccccg ccgccgatca
    79261 ccgcgaacta tcatgaccgc gccaaattcg aaaggaatgg cccatagaac gcgatgaaca
    79321 cacataagcg ctaacttgct tacctgaaaa cttaacgtcg ccacagagcg attgagagcg
    79381 cttaaaatcg atgcgatttc attaaatttt tctcactcgc ctgaatggtg gcgatcagga
    79441 ctgatcgcaa cattgccccg cctcgatatt gagcgcataa tagatacata cattaagcag
    79501 aaaagagata agtttattgt gaaaatcagg gggttacaca tcatgcgcat atcagaacaa
    79561 caacggcaga tcctcgacta tctggcaaaa aacgaatcaa tcaccgcacg acaagccgca
    79621 gatctcgttt atggtggcaa tgttacccgc gtccaggttg acgccgcccg ccgctcactc
    79681 atgacgatgg ttagtaatgg gctaatcagg aaacaaggcc gcgctttcgt agctgaaaga
    79741 catatcaatc acgcttatag ccaggacgtt gaagaattgc gccgccagat cctttgggta
    79801 ttcagcaaac agggtcagga gtatttcctt tctgaacacc caaatttcgc cgccaaagag
    79861 ttaacagtaa gcggattgtc gtgcgcactg gattatcttg tcggtgaagg ttacgttact
    79921 tacaagcagc tatggcacgc cctgaatacg cttgtagaag atggtttact cattgccacg
    79981 cgtgagaaca tcgaatactg gataatgccg cgttcaggtc gccgcaatca tgcacggtat
    80041 atttcaggca acagatacag aacgccggac tttaatccga cacaggaaga tcgggaccgt
    80101 gccgaaatga tgtgcaggga ataggagaaa agaattatgg ataagttatt ttcgatgcag
    80161 tgaacacaag cgggcgcaat gcccgttttt attgccttat tccacgcctg acgaatcaat
    80221 ttgatcacac ctgagcaaat gcatagccag aagttaaggc cgttatattg cgattgggag
    80281 cgttttaaac ggtatcaatt taattaattc tgatgtggtg atttgcaggc aaaaaaaacc
    80341 cgccggagaa gcgggaaata gagacatatc gttgtagtta ttaacagatc actttgaggt
    80401 cgagggcggc taaccctcgc taatattatc ccgccattac cttgcgggtg caaataatct
    80461 tttattgctt tgatgattta ataaatagca ctttttgcta acaaaaaccg ctgtcaatat
    80521 atggccaaaa atgtcaacag aaaaaataag gtttataata ttaagtatca catgggtaat
    80581 gggttcaggg aatcggaatc caccgcctca cggaaattca aaatttgctt cttctttcct
    80641 ccgtggggcg gcttcttttt cgtatcgagg tatttatgaa agcaataaca ttagaacagt
    80701 tcgcagcaat ggcgaatgta tcgaccagaa cgctgaggcg ctggctaaaa aaaccatcaa
    80761 ccggagtccc tttgccattt accccacgcg gtactcgcca attattctgg gagcacgaat
    80821 gcaaagaatg gttgtacaga acagaacgat aataatcacg catcacattt tgaggtaaac
    80881 atcatgacca atatcgaaag attagaacgc tgtgccgata ttatgcggcg cagatggatt
    80941 tatgaccctg aagcgggttt attgtttagc cgtgaaacaa aagacgttat caaaggcagc
    81001 atgaccaata aagggcattt ggttgtaacg gtacatgaag gtaaattcaa agtgacactc
    81061 acctatcaga aagcctgcta tgtatacaca tacggcgctt atgacgagac attatatgaa
    81121 gtgcaccacg ttaatttgga caaacaggat aaccgcatca agaatattcg cctgatgccg
    81181 aaagaaaagc accgccggat acataaaaac gtccgcaaac ttattcggct gggccacaca
    81241 caatggcttc aaccgcaatt aattgaacaa tcactataaa cacagtagta aataccatgc
    81301 attcattaga cagttatttt cagcgcacga cagcccctaa atcagccgcc caggaacgcc
    81361 gtgaggaatt ccatgagaaa gtgatgcgtt ctgctgatta tattgcggac aagttcgtgg
    81421 aaacggttcg tccacttgtt gatgaagtag ctgataaact tcagtctgaa atgccggaag
    81481 atatggaagg cactgcaaag cggcgactca tttgctaatt aagtcgtcgt tttggcgtgt
    81541 caataagcgc ttttaaataa tcgaaaaaag gggggaaaga taatggacgc atttgaggta
    81601 ctggcaaata aaggcgctga actggtagca cagcgtgata aaaccgccaa cgaaggggaa
    81661 cgcgcactgc taaataaaca gattaaagcg atccgtatgg cccagtttaa attaatcagt
    81721 aatgaagtta tcgaaacaat taaaccgcag atcccacaaa tcgttgctga cgctattaaa
    81781 gccgccggac tggagaaacg cattgccggg ctaaaaacga acgcagagaa aatggagatg
    81841 cacaaacaga gcggtgctcc gaatcccgat gagtacttct caccggatgt tgagtttatg
    81901 gcgcaggttg aggaacgtct gggtagctgt ctcaccgagg aacagcgtcg ctattttgat
    81961 ggagtggata gttcggcagg tattgacctg aactcgtatt tcggtcgtga aattgagcat
    82021 tttgatgccg ccccgtctat gccggagcca gaaccagaaa tggccaccga tcctgaagct
    82081 gagcaacgga aacgcgtatt caatgcgata gggattcggt actgatcgga taaaagaaag
    82141 ccgggaatta ccccggctga cttaacaatg tgaccaatga acaaatcgga caagacaaac
    82201 aggataagca gtctcgaaac aatgttcggg aactatatta gcacaagtcc ggagaaaatc
    82261 atcatgttgg cactacgcat catgcagggc atcgctaaga ccctcgcaga acatgtctta
    82321 gacctgaaac attcgccact gagcaaacag gccatgaagc gctaaacgct tcgtctgtgg
    82381 gctgaatatt cgcttggaac tatcaataaa atcatcgaca tgaaatcggg gccgagcaac
    82441 cagagcgcgg aggaaatgga gttcatcaag cgtttaattc tcattcgcag agacattcat
    82501 agccagttac acagcgtggg aatcgatatt aatgatggga cgggcgatta attttcggac
    82561 aagtactatg cgcgcgcgag gagctgaaca tgaagcaaga tattgcagac aggctggaga
    82621 tactcgaagg ccagcgggca gaagcgaaac aactacgtaa gcaggccaga agagcgcaca
    82681 ggaacaacga agcagagtta ctaaccaaat acatcagttt caccaattac tgcatctacg
    82741 agtgttacaa ggaagatgca gaggactggc tggattcatt gccagaacag tactgagcag
    82801 aataacacca cgtcatgttg ttctgaggtg agcatatcac ctctgttcag gtggccaaat
    82861 ttagtgtgcc acttcagcat caccgttgaa atcacaataa cgattttttt gtaaatatgc
    82921 tctcacatat gtaggaatag accaggttgg cattgaaaca catctaccat gaccataata
    82981 aagtaatttt ttgatatgtt tatggtttta tattaattaa aatatatcta aaattgatag
    83041 ttttaaaatt gcaaaatcca gattgtgttt gtaatgttta atctgattgt attgagtggc
    83101 gtttattatg aaaaataaat caggtaattc ttgttataga gaagcagtcg atattcatag
    83161 ctcacttgag ctttcattta tattgcataa atatcatttg cgtgtagatg agaagaaatc
    83221 tcgaaatgta ttcagcgcca tacgtgatga gctcatcctt gatggggatt cacgacaaaa
    83281 cttagctact ttctgtcaaa catggcttga tgatgaaatc cgtgagttgc ttaatttagc
    83341 tattgataaa aacatgattt ataaagatga atatccacaa atggcggaac ctgaaggtcg
    83401 ctgtgtgcgt atgctttccg attcgtggag tttccccgat tcgcgacaca ccttaggttg
    83461 ctcaaccatt ggttcatctg aagctgctat gctcggccgc ttggcgttaa aatggcagtg
    83521 gtgtaaaaaa cgcgaagtac aaggtaaatc aaccgaacct gatttgcgat cctgtgcaaa
    83581 tatgctggca taaattctgc cgttactgtg atgtgaaatt gtgtgaaatt ccacttgatg
    83641 atcatcacct gataatgaat gctaaagagg ttttgaagca tgtcgatgaa aacactattg
    83701 gtgtcgttac gactttaggt attacttatt tttgtaaagc agattgcgtt agtgttttta
    83761 agataagtgt taaagttaat attagttttt atatttacat cgtaattata tgtttctatt
    83821 ttaaaaatat tcgagagtat aaaaatgtct aatgctaata tcgcacccaa aagtaagaaa
    83881 atgacagtgg ggacactcgc tatcatgaat ataactgctg tagtaagtct acgatttata
    83941 ccttctgaag cagaatacgg cttaggtgcc attttttact atacctttgc tgcaattatg
    84001 tttttagtac ctgttgcctt tgttgcggct gagctagcta caacatatcc acaaaaaggg
    84061 ggggcatttc gatggatagg agaagcattt ggggctcgct ggggttttgt aggaatgctt
    84121 atgacctggc tacaagtcat cccctacttt ccaacagtac tgacatttgg tgcagtctct
    84181 gttgcattta ttgatgttga tatgggggtg gcagaatcaa ttgccagtaa taaatggtat
    84241 attttatttt tcgtactatt tgtttactgg ggagctattt gtgttgcatt acgtggcgtt
    84301 ggtattttct cgcaggtatc taagtggtgc ggtattatag ggacaattat tccagctatt
    84361 atccttgtta ttcttggttt ctcctatctg tggttcagcg gtaaaccagc ggtgatccca
    84421 ttaggttggg gtgatctttt ccctaatata gctaacttcc agaatattgt tcttgccgcc
    84481 agcatatttc ttgcttatgc agggatggaa atgaatgcgg tccatgttaa cgatttagat
    84541 aatccaacta aaaaataccc aattgcaatt accatcgctt ctataggtac tattgcaata
    84601 ttcctgctca gtactttagg ggtcgccttt attattccta cagataaaat tagtctaact
    84661 caaagtttgt tggttgccta cgatatgctg ttcgaatggg ctggagtgcc atggcttggc
    84721 tctgtgatgg catttatgct agcaattggc gtactgggtg gggtggttac ttggattgca
    84781 gggcctaata caggtgtatt agcgatcgcg aaagctggtt atttacctaa gtttttccaa
    84841 aaaaccaatc gtcatggaat ggggcaccat ctaatgtttg tacaaggtat aattgtctca
    84901 gttctttctg ttacttttgt gataatgcct tcagttcaag cagcttttca gattttatca
    84961 cagttaacag ttatgctcta tttagttatg tatatgctca tgttcgctag tgctatctat
    85021 ctacgacatt ctcaacctga tacaaaacgc ccatatcgag cccctgcttt atttttctgg
    85081 gcaacacttg gattcttagg gtcttttctg gcttttgtat taagttttat tccgcccgag
    85141 caaatcccaa ctggtagtcc aaccagctat gttctttatt tgttagtatt agttgcccta
    85201 tttttattaa ttccaatatt aatttacgcc ttccgaaaat ctgactggaa agatccaaac
    85261 tctgatttcg aaccattttc ttgggaaaaa agaactagta attaaatttt ccataagcta
    85321 caaaagatga ggttaaatat gtttagtgct gaatctccaa ttggtgtaat agactcaggt
    85381 gtaggtggat tgacatcggt aaaggaaata attaatttgc ttcctggtga agatattatc
    85441 tattgtggag ataatatgaa tgcaccttat ggcaaccgtt ctgccgatga tatcatcagt
    85501 ttgactaaaa aaatgctgac ttttttacag tcaaggaatg tcaaactagt tgctgtcgca
    85561 tgtaatacta tatcctcaac tttggaatcc gaaaaataca gcggatatgc taagagcttt
    85621 ccgtttccta tcttatccat cattgaacct gcagtagaag atgtgattcg ccagcaatat
    85681 aaaaatgttg gtattatcgc taccgagttt actattaaaa cggggtgtca caaagagcta
    85741 ataaaaaaat tgaattcaac aataaatgta tttggagaac caagcaaaaa tcttgcaatg
    85801 ttaattgagg aaggtaatct taatgctcct gcaatcttga atgacatcaa aaaacatgtt
    85861 aaccatttaa tttccctcca tccagtaaat gaaattattc tcggctgtac tcactatcca
    85921 attgttcaga atttttttga agcagttgca ccggatataa aatttattaa tcctgcacat
    85981 gatcaggcta tctctattaa aaatcacttg ggacagctaa atttattaaa ctcctcaaat
    86041 ataggaactt tgcaaataaa tactagtgga agtatggaga tctacaaaac ggtactaggg
    86101 gaattgagca ttacgaaacc tcatacattc agtatcaggc agttttaaat ttatccggtg
    86161 caccgtaaaa tcccgtcctt ctgaactgca ccccaaaagt tggacaccaa ctgagtaagg
    86221 tgcattttat ggacaaaccc agttacatac tgggtagtgc atccaattag tagaacatgt
    86281 gtttttcgat aaacgctccg atcacttttt cgtggatctc aactgagcgt gagaagcaga
    86341 ttgttttacg acccaaccgc ttaatgcggg tgcgtagcgt cagattatta cgctcaatgc
    86401 gttgggtgaa tattttgccg gtcagatgct tattcttcgg cacctcccgg ccatagctgc
    86461 cccagtcatc gctggtcagc atgccgatgt tgaagggtgg cagtgccagt agctcccggc
    86521 acgtttgatc ggttcgggga ccaaaagtgt aggccagtac accgcctgtt ttggtgttgt
    86581 acgcgtaccc gagccaatgt tgccgggctt tactgccaac gtagctccat tgctcatcaa
    86641 gctcgcagat aaacgccaca tcagcatggg caacaggcga agaagttatt cgctttggcg
    86701 cgagtttttt aaagtccgga tgacggtgtt actaccaatt ttcagtgtcc tggcggtatc
    86761 gcgaaccccg gcaccattaa aggccatttc agttatcagc tctttcatac ccggcttacg
    86821 tgcttgataa gtataagtga actgaaacac acggtggcag tcacggcagc gaagtctgtc
    86881 acggccttta gggttctgac catggcggta aacctgagct gactgacaac ggggacaatg
    86941 aatattaacg ctggtcatga gataacctca aaagcccgta ttatacatca gattcaacta
    87001 attagaggca tcacccatcc agcattataa aacatactaa caataactaa attataataa
    87061 tcacaactta caatcaaata ataaatgcat aagattaaca atgatttttt cgggttatat
    87121 ttatctaaaa aagcagaata ttatgtaact tattattttc taagcgaaaa tttattgtgc
    87181 agtcagacta gcaaacttgc gtaccacccg aagaacatgc gaagatttaa gtattctttc
    87241 ttttttggtt tttatttttc attttctctt ttcttctctt tccggttctc ttctgttctg
    87301 gttttggttt tttccttttt atttttattc tttcttcagg gttatgctaa aacaccaaaa
    87361 catttttcac cacaaaagca tccaatcatc ttgccagacc aatcacctta accggataca
    87421 ctgccggata taatcccact aacacccagc caccaagcac catacctgat aactaccgta
    87481 ttcaggaagg aaatgcgagt gatttcaccg tcaccggcac aaccgatccg ccaaaaagag
    87541 gcgtaccaat gtcgatatag tcccccgcgc ggacaatcac ttcgtcaatg actgccaacg
    87601 ggagttgttg tagctgtggg cgcaacaaca tgcccagagc tttaccaaag tcctgcccgg
    87661 ccaccaccag caggggatgc ggattcggaa aacgcgcgac gaaatcgacc agcgcgttga
    87721 tgaccgtcag taccgcggcg taacgcacag gcagcgaggc gggaagcgcc agcacgtaca
    87781 cgtcagtttt gggatcaaga tccagctgaa gcagcgcctg ttgccaggca ctcaccagat
    87841 ccgtttcatc aatcgggatc gccaccggca aattgcgcag cggcagttgt acgccctcca
    87901 gccagattgt gctgccagag agcgaaaggg tatgtgcacc cgcgccaatc accgtggcgc
    87961 atacggtttg cgccggaaac tgcacattca tctcacgcag gcgcggatgg tcatgcagcg
    88021 ccgttgccag aagtgggcca atatcggcaa aacagaatgg gtcggcgggc tggtggcgat
    88081 aacattcgcc cacacctcca gaaagcgtaa tgatttcggg cgtaacacct gcgggcagca
    88141 aaccggtttg catcaatgcc tgcgcgagcg gcgaaagcgt tccgtcaatc acttcgacaa
    88201 tcagcgccgc catccgccgg gtaacctgca ccagctgcgc gccggtcagc gaacgggcgt
    88261 cagtgcctgc accgaagcac tcatccacaa tcatctgccc cggtttatga gcgtaaacca
    88321 cgcgcccctg gctgtcggtt tccagcaggc gaccaccgac gttgagacag gcagtgccgc
    88381 tgatttttcc ggcatcgaac agggcgtagt tcgcggtgcc accgccgata tcgatattca
    88441 gtacccgaca cagccgttgt tcagaaaggg tttgcgcccc ggctccgtga ccggcgatca
    88501 cggattcgag gtgcggcccg gcgctggcga cgacaaaatc gcccagcgac tgtttttcct
    88561 tcatcacttt ttccatcagc tgtgcgacca ggctttcggt aaactggcct tccggcagct
    88621 gggcaatgat ggtttcgcgg atgcgctggc tttcggtttt gtcggctggc acgctggcgg
    88681 gcacggacgc cgttaccggg atcgattcat cacattcggt gatggtaaaa cccagtagat
    88741 ccgacacttc acgggcttcc ggggtaataa tgctggcgcg cagaaccacg gacattgcct
    88801 gttcgccgcg cgcgtgtgct tcacgaatat cattcgctgt gatgagtttt ttcacctgct
    88861 tcctcctgtt tggtgagaga tgccagataa tccaccagtt gctgcacgct ttgcgggtcg
    88921 tggctgttta gctcgaaaat cggctcttcg aaacccgtct caagcaataa ctttcgcgtt
    88981 gcggcgacgt cagcatctgg catgtccgtt ttactgatga cggcgatttg ccgtttgctg
    89041 acgccaatat ccagcaaccc ggcaggcaac cgactttccg ggtcatttgc gccatggaca
    89101 tagatcagca tgtctacatc ctgtagcgtg gtaattaagg cgtgatacca gcggggatgg
    89161 ctaaaatatt cacccggcgt atcaatatcg cccttatcat taaattccac ggcctgtgtt
    89221 tttctggcga gggtataatt cccctgcaac gcattaaata acgttgtttt tcctgccccg
    89281 acgctaccca caaacgcaat tcgtttcatg aatgcctcgt attaactttt ggtcatttca
    89341 caaagcgtat aatttaataa gcgacctaaa ccgctgacgg tttgtgataa cgcttcttct
    89401 actgcgccca ccgagccata aatcaccaat gcaccgctga agcggtcgag aaaaccgata
    89461 tgcacatcag cggctttcag cgctaaatca ccggcgatca tcgccgtttc gccgggagtt
    89521 agcgtcataa tgccaattgc gcccgcatcc ggaacgccga ttttcttcgc cagttcctca
    89581 ccgggatgcg cgatgagatg cgccagtgtg acctgtttgc cgggcacaaa ttcctgaatg
    89641 atgcgttctt tatccatgag tcgccgcctg tgaaaaactt tcgtgtatcg tgacaatttt
    89701 tcgtcagagt aaataacaaa attcggcaag cgcgtttaaa aggtgaggta gatcactaag
    89761 aatggagaga tatttactgt tatgaataaa ctttcatatg cgacgcatga atataaataa
    89821 aataatatcg agatatttta tatactgatt tttttattat tcgcaaactc agtaataagc
    89881 tttgctaata tatttcagga aaaccgctta ccctgaatat tcagggtaag cgtgagagtt
    89941 aagaatgaat tacagcggtt gggtttgcgc ttcaaccacg gccagcgcca ccatattgac
    90001 gatacgacgc accgatgcga tcggcgttaa cacgtgaacc ggtttcgcca cgcccatcag
    90061 caccggaccg acagtcacac cttccgagct ggaaacgcgc agtaagttgt aactaatgcg
    90121 ggcagcttcc atgttcggca tcaccagaat attggcagaa cctttcaaag ggctgtccgg
    90181 catacggtcg ttgcgaatcg cttccaccag cgctgcatcg ccgtgcattt caccatcaat
    90241 catcagatct ggcgcacgat ccctgaccag ttccagcgcc tgacgcattt tgctcgacga
    90301 cgggcagtca gaagaaccaa agttggagtg cgacaacaga gcaacgcgcg gctcaatacc
    90361 aaaacgacgg acagtttctg ccgccatcaa ggtgatctcc gccagctctt ctgcgtctgg
    90421 ttcgtcattg acgtaggtat cggcaacaaa ggtgttacca ctcggcagca gcagcgcgtt
    90481 cattgcacct gcggtgtgaa cgccatcgcg ataaccaaag acatttttca ccacgctaaa
    90541 atgttcatga taatcgccca ccgtaccgca gatcattgca tcggcttccc cacgctgaac
    90601 catgatcgcg ccgatcactg tcgggttact gatcagcgca cgctgcgcct gttcctgagt
    90661 gacaccgcga cgcttcatga tctggaagta ttcggtccag tactctttaa agcgcggatc
    90721 ggattcgtta ttgacgattt caaaatcaac gcccgctttg atctgcaagc ccagtttctg
    90781 aatgcgcatt tcgatcacgt tcggacgacc gataaggatc ggtttcgcca gtcccagcgt
    90841 taccagttcc tgagtcgcat gcagaacgcg cgcctcttcc ccttccggca gaactacgcg
    90901 cttcggcgct ttgcgagcct gggagaaaat cggcttcata aacaggttgg ttttgtaaac
    90961 gaactcagtc agcttgtcga tgtagacgtc gaaatcagca atcggacgcg tcgccacgcc
    91021 cgactccatc gccgcttttg cgaccgcagg agcgatctta acgatcaagc gcggatcaaa
    91081 cggttttgga atgatgtatt ccggaccaaa gctcagatcc tgaacgccat acgctgaagc
    91141 caccacttca ctctgttctg catgggccag ttctgcaatc gcgcgtaccg ccgccagttt
    91201 catctcttcg ttgatggcgg ttgcgccaac gtcaagcgcg ccacggaaga tgaacgggaa
    91261 gcaaagaacg ttgttcacct ggttcgggta gtcggaacgg ccggtacaga tgatggcatc
    91321 cggacgcact tctttcgcca gcggcggcag aatttccggt tccgggttcg ccagcgccag
    91381 gatcattggt gcccgagcca ttttcttcac catttcctgg gtcagcactt tcgggccgga
    91441 acagcccagg aaaatatccg caccttcaat cacatcatcg agggtacgtt tgccgtcatc
    91501 caccaccgca tatgcggctt tggtttccgc catatttggc tcacgcccct gatagataac
    91561 gccttttgaa tcgcaaacca cgatgttatg tttttgcagc cccagcgcta ccagcaggtt
    91621 catacaggcg attgctgcgg cacccgcgcc ggaaaccacc atccgcacgt cggagatgtt
    91681 tttctccacc acgcgcaagc cgttgaggat ggcggcagtg ctgatgattg ccgtgccgtg
    91741 ctgatcgtcg tggaataccg gaatattcat ccgctcgcgc agtttctgtt caatatagaa
    91801 acattctggc gctttaatgt cttcgaggtt gatgccgccg aaggtcggtt cgagcgcggc
    91861 gacgacttca ataaatttgt ccgggtcgag ttcatcaact tcaatgtcaa atacatcaat
    91921 cccagcgaat ttcttaaata gaacgccctt gccttccatc accggtttgc ctgccagcgc
    91981 gccaatgttg cctaacccca gcaccgccgt accgttagag atcaccgcaa ccaggttacc
    92041 gcgcgcggta tatttgtagg cttttagcgg gtctttttcg atttcaagac aaggtgcggc
    92101 aacgcctggt gagtaggcca gcgccagatc gcgctgcgtt gccagaggct tggttggaga
    92161 aacctggatt ttccctggaa ctggaaattc atggaaatca agtgcacttt gttttaactg
    92221 gtcatccatt tggttgttcc tttcacgtaa cgttcacaaa taaagtgtgt gggcaacagc
    92281 ccctgcccac aacgtggcgc acattattac cctgccggag tctacagact ttgagcaagt
    92341 ccaaactctc accattaata taatgttttg gtaacaatcc tataacactg atattacctg
    92401 cttaatccag caataccatg cctgtctgct atgctttttt gatgcgttta gcgaaatttc
    92461 tcagaagtgt gaattaacgc actcatctaa cactttactt ttcaaggagt atttcctatg
    92521 aacgagttag acggcatcaa acagttcacc actgtcgtgg cagacagcgg cgatattgag
    92581 tccattcgcc attatcatcc cctggatgcc accaccaatc cttcgctgtt actcaaggct
    92641 gccggattat cacaatatga gcatttaata gacgatgcta tcgcctgggg taaaaaaaat
    92701 ggcaagaccc aggaacaaca ggtggtcgca gcgtgtgaca aactggcggt caatttcggt
    92761 gctgaaattc tcaaaatcgt acccggttgc gtgtcaacag aagttgatgc acgcctctct
    92821 tttgataaag aaaagagtat tgagaaggcg cgccatctgg tggacttgta tcagcaacaa
    92881 ggcgttgaga aatcacgcat tttgatcaaa ctggcttcga cctgggaagg aattcgcgcg
    92941 gccgaagagc tggaaaaaga aggtattaac tgcaacctga cgctgctgtt ttcttttgcc
    93001 caggcgcggg cctgtgcgga agcaggcgtt tttctgattt cgccgtttgt agggcgtatt
    93061 tatgactggt atcaggcacg caagccgatg gacccgtatg tggtggaaga agatccgggc
    93121 gttaaatcgg tgcgcaatat ctacgactac tataagcaac accactatga aaccattgtg
    93181 atgggcgcga gcttccgtcg caccgaacaa atcctcgcct taaccggctg cgatcgactg
    93241 actatcgcac cgaatttgct gaaggagctg caggaaaaag tttctccagt ggtacgtaaa
    93301 ttaatcccgc cttctcagac gttcccacgc ccagcaccca tgagcgaagc ggagttccgt
    93361 tgggagcaca atcaggatgc gatggcggta gaaaaactgt ctgaaggcat tcgtctgttc
    93421 gctgttgatc aacgcaaact ggaagatctt cttgccgcca aactataaac cagccacgga
    93481 gtgttatatg tcccgaaaag accttgccaa tgcgattcgc gcactcagta tggatgcggt
    93541 acaaaaagcc aattctggtc atcccggcgc gccgatgggc atggctgata ttgccgaagt
    93601 gctgtggaac gattttctta aacataatcc taccgaccca acctggtatg atcgcgaccg
    93661 ctttattctt tccaacggtc acgcgtcgat gctgctctac agtttgctac atctgaccgg
    93721 ttacgacctg ccgctggaag aactgaagaa cttccgtcag ttgcattcga aaaccccagg
    93781 ccacccggag attggctata cgccaggcgt tgaaaccacc accggcccgc ttggacaagg
    93841 tttggcgaac gccgtcgggc tggcgatagc agagcgtaca ctggcggcgc agtttaacca
    93901 gccagaccat gagatcgtcg atcacttcac ctatgtgttt atgggcgacg gctgcctgat
    93961 ggaaggtatt tcccacgaag tctgttcgct ggcaggcacg ctgggactag gcaagctgat
    94021 tggtttttac gatcacaacg gtatttccat cgacggtgaa acagaaggct ggtttaccga
    94081 cgatacggca aaacgttttg aagcctatca ctggcatgtg atccatgaaa tcgacggtca
    94141 cgatccgcag gcggtgaagg aagcgatcct tgaagcgcaa agcgtgaaag ataagccgtc
    94201 gctgattatc tgccgtacgg tgattggctt tggttcgccg aataaagcag gtaaggaaga
    94261 ggcgcacggc gcaccactgg gggaagaaga agtggcgctg gcacggcaaa aactgggctg
    94321 gcaccatccg ccatttgaga tccctaaaga gatttatcac gcctgggatg cccgtgaaaa
    94381 aggcgaaaaa gcgcagcaga gctggaatga gaagtttgcc gcctataaaa aggctcatcc
    94441 gcaactggca gaagagttta cccgacggat gagcggtggt ttaccgaagg actgggagaa
    94501 aacgactcag aaatatatca atgagttaca ggcaaatccg gcgaaaatcg ctacccgtaa
    94561 ggcttcgcaa aatacgctta acgcttacgg gccgatgctg cctgagttgc tcggcggttc
    94621 ggcggatctg gctcccagca acctgaccat ctggaaaggt tctgtttcgc tgaaggaaga
    94681 tccggcgggc aactacattc actacggggt gcgtgaattt ggcatgaccg ctatcgccaa
    94741 cggcatcgcg caccacggcg gctttgtgcc gtataccgcg acgttcctga tgtttgttga
    94801 atacgcccgt aacgccgcgc ggatggcggc actgatgaaa gcgcggcaga ttatggttta
    94861 tacccacgac tcaattggcc tgggcgaaga tggtccgacg caccaggctg ttgagcaact
    94921 ggccagcctg cgcttaacgc caaatttcag cacctggcga ccgtgcgatc aggtggaagc
    94981 ggcggtgggc tggaagctgg cggttgagcg ccacaacgga ccgacggcac tgatcctctc
    95041 aaggcagaat ctggcccagg tggaacgtac gccggatcag gttaaagaga ttgctcgtgg
    95101 cggttatgtg ctgaaagaca gcggcggtaa gccagatatt attctgattg ccaccggttc
    95161 agagatggaa attaccctgc aagcggcaga gaaattagca ggagaaggtc gcaatgtacg
    95221 cgtagtttcc ctgccctcga ccgatatttt cgacgcccag gatgaggaat atcgggagtc
    95281 ggtgttgcct tctaacgttg cggctcgcgt ggcggtggaa gcaggtattg ccgattactg
    95341 gtacaagtat gttggtctga aaggggcaat tgtcgggatg acgggttacg gggaatctgc
    95401 tccggcggat aagctgttcc cgttctttgg ctttaccgcc gagaatattg tggcaaaagc
    95461 gcataaggtg ctgggagtga aaggtgcctg atggtgatcg ccggatgctg tatgttcccc
    95521 tcaccctaac cctctcccca gaagggcgag gggacggtcc gtgcgctttt tagggccgca
    95581 tctgcgattt tgtttaacgg gtgatcacaa cgtgggcctg gcgtctggcc catgccagtt
    95641 atcgcaggta ggttctgcgg cgtaacgcac caggcgaaaa ctctgaccgt caaagcgcca
    95701 gcgcgcctga atgccacaat cgcttaatcc gcgccctttc gctaaggtca ccagttcacg
    95761 cgatttctca tcaaatgttg cgttcatcag ttccagttca ttcgtctcct gaccgttgtt
    95821 gaacggcaaa cgcaaccgaa ccgggcgcga ggctagcggc tttttacgcg acacaatcca
    95881 tgccaaatca atggtgttat aggcccctgc ctcacagcta atcatcatca gtgctttatc
    95941 atcggtcagc gcagtgacat tcacctcacg acgtaacgga tcaagcgagc agcgcagacc
    96001 attcatccgc cagtttccat aatccagcaa atcgttacgt tcttcgagtg agagtggcgt
    96061 cggcgttggg ttaaccaccg cgacctcttt cagcgcaggc gcgggcggta cgctgagagg
    96121 cggttcgtcc cctttcttga tccacgcggt ttcactgcca acgcgctttt gctgagcatc
    96181 aataaacaac aacgccgctt ttaagccgct cagagaaatg gtctgattgc cgtcgcgtaa
    96241 ggtgatcgcc ttcccttcct gaattatctg caaaaacgcg gtgatggttg ccgtatcatc
    96301 ggttactaat aaccatggtg aaatccgcca cttgtcacca cttaatgcca acggctcacc
    96361 atctaatagc agccgtggcg ctatctcccc ttcagacgca tccggcgact tcaacccacc
    96421 gcgctcaata cgtaaaacgg catcggtatg cgccccagcg ctgcggctca gggtcatcac
    96481 cagtccatta tgatcgcccg tattgcgcgc cacacaaaaa ttttgattat tacaggtgac
    96541 ctgccagtcg gaaaacgccc gttgcgctgg tgccgcccac accaaagacg tcggcaacaa
    96601 agcgaaaaaa aagagaagga aaatgcgata gcgcatggac ggtacgaccc cagaagttca
    96661 aacaaacagc acaagtcgta tcttcgttgc gcaggtagcg cggctcaatc ggatttgtcg
    96721 gattgagcca ataataaaca acgaaatatt attgctattt ttcagtccat taaatgtgac
    96781 atttgcaaat agttaagcaa taacaccgtc ttaccgtcac gtatctcgcc ggttttgatc
    96841 atctccagcg cctggctgaa cggcagctcg agcacttcaa tatcttcatc ttcaacaccg
    96901 ccaccggcgt tagcgcgctg attgtcactg tattcggcga taaaaaagtg gattagctca
    96961 gtcacaccgc caggcgacat atacagttca aataatttgc gcacttcgcc cacttcatag
    97021 cccgtctctt caatcgcttc tttacgaatg cacacttccg gttcgtcgtt atccagcagc
    97081 ccggcgcagg tttcaatcag ctgcccgctt tcattgccat taacccaggt agcgacacgg
    97141 aactgacgaa tcagaaccac gctctttttc ttcgcgttgt acaggaggat cgtcgcacca
    97201 ttaccgcgat cgtaaacttc acgtttatgg cggataactt caccatcttt gcgcgtgaga
    97261 tcgtaagtaa tgttgtgcag ggtgaaatag ttatcggaga gaattttgtc tttaatgagg
    97321 gtgatttgtt gcgtcatacc gactccacag cgcgaaatga acaattatat tacgctgtga
    97381 agtcgggttt gtctgcgcag gctatcagtg agatttgact gattttaccc ccagccagtc
    97441 aataatcccc tgtgccgcat gacgtccttc tgccattgcg gtaaccacca gatccgcacc
    97501 acgcacggcg tcgccaccgg cgaagatttt cggattggtg gtctggtaac ggtactggct
    97561 ttccacatcc gcgatgatgc ggccccattt gtctaccgtt acaccgtgcg actccagcca
    97621 cggcatcccg tgtggattga agccaaacgc cataatcacc gcatcggctg gcatgacaaa
    97681 ttcactgccc tcaatcggta caggtcgccg acgcccctgg gcatccggct cgccaagacg
    97741 cgtgcgcagg aaacgaattc cgcaaacgtg accttgctca ttcagctcaa gcgccaccgg
    97801 ctggacgtta aattcgaagt tggccccctc ttcgcgggcg ttcttcacct ctttcttcga
    97861 gcctggcatg ttagcttcat cacgacgata agcgcaggtg acgttactcg cgccgtggcg
    97921 cagcgcagta cgcacacagt ccatcgcggt gtcgccgccg cccagtacca cgacgttaag
    97981 tccggcggta ttgataaacg gctcttccgg tagctcttcg agccccatca cctgtttagt
    98041 gttggcaatg atgaacggca gcgcgtcata aacgcccggc gtatcttcat tgggtaaacc
    98101 cgctttcatg gaacggtaag tgcctacgcc aacgaagacc gcgtcgtatt gttccaaaag
    98161 cgaatccaaa gagacatctt tacccacttc acaattgagt tcgaagtgaa tccccatcgc
    98221 gctgaagatt tcccgacggc gtgccagcag ggatttatcc agtttgaaag aaggaatgcc
    98281 gaaagtgagc aagccaccga tttctggatg gcgatcgtac accgtcaccg ccacgccatt
    98341 gcgggtcaga acatccgcac aggccagccc tgccggacct gccccgataa tcgccacccg
    98401 cttgtccact ttggtgacat gacttaagtc aggacgccaa cctttcgcca acgcctgatc
    98461 tgaaatatag cgttcaatgt tgccgatagt taccgcgccg tgctcatcgc gaatagtaca
    98521 ggcaccttca cacaaacggt cttgcggaca aacgcgtccg gtaatttccg gcagggtgtt
    98581 ggtctggtga gaaagctcga cggcggcgtc gatgtttccg gctttcacca gttcaatcca
    98641 ctgcggaata tggttatgca gcgggcaggt ccattcacaa acgctgtgct cgccgaactt
    98701 aaggcagcgc gaggcttccc gttgtgcctg gtcggcgcga aatggcagat aaatttcatc
    98761 aaaaccggtt ttgcgcgctt caatcgccag tttatccggc tcgccacgcg cgggcgttgc
    98821 ctgcatttgt tcgactttac tcattaccgg catttcttgt gcagcggtac tggcatgcca
    98881 cggttgatgc tcctgacgtg cggtgcgcaa gcggcgggat ttcgccatgc cggagagtgc
    98941 gacgtcagtg accagttgca gcgcgtccgc cgggcaattc tcgacacagg caggaccgtt
    99001 ttcgcgccct gcacaaaggt cgcatttatg cgccgtggct tttacttttc ctgccgcgac
    99061 gggtgtcagg acgatttgca tcgtaccaaa cggacaggcc accacgcagg atttacagcc
    99121 aatacacttt tgctgattga cctgaatgct gtcatcaacg tggctgattg cgccattagg
    99181 gcagctacgg gcgcagggcg catcttcaca atggtgacag gtcactgcac tacgttgctg
    99241 ctgatgtttg ataaccgtaa ttcggggatg aaaatggtgt tggctcagga catgttgctc
    99301 atcattgtga gccatgacac aggcgatttc acaagcatga caacccagac attgctgact
    99361 gttggccata ataaaacgat tcataacgac cttctttttt ggttgtaaaa accttattct
    99421 ttatatgagt gttgttatta cccgacttac aggggaaccg gcaatgttca tgtgcccaga
    99481 ataagtaact atttcgctat aaactgtggc agatcaaata atcccatcac tgactaaatt
    99541 gcgtttcagc gaactggaac attaatgatt ttttgtggag aagacgcgtg tgattgttaa
    99601 acgccccgtc tcggccagtc tggcccgggc ctttttttac attgtgctgc tgtcgattct
    99661 ttccacgggt atcgctctgc taactctggc gagcagtttg cgcgacgctg aggctatcaa
    99721 tattgccgga tcgctgcgta tgcagagtta ccgcctgggc tacgacttgc aaagtggcag
    99781 tccacaactc aatgcacatc gccagttgtt tcagcaggca ctgcattcac cggtattaac
    99841 caacctcaac gtctggtatg tgccagaagc agtaaaaact cgctatgcgc atctgaatgc
    99901 caactggctg gagatgaata atcggctcag caagggcgat ttgccgtggt atcaggccaa
    99961 tattaataat tatgttaatc agatagacct gttcgtgctg gctttacagc actacgctga
   100021 acgcaaaatg ctgctggtgg tggcgatttc cctggctggc ggtatcggta ttttcacgct
   100081 ggtctttttt actttgcgcc gcatacgcca tcaggtggtt gccccgctga atcagctggt
   100141 taccgccagt cagcgtattg aacacgggca gttcgactcg ccgccgctgg ataccagtct
   100201 gccgaatgag cttggtctgc ttgcaaaaac ctttaaccaa atgtcgagcg agctgcataa
   100261 attgtacctt tcgctggagg cgtcagtaga agaaaagacc cgcgatctcc acgaggccaa
   100321 gcgtcgtctg gaggtgttgt atcagtgcag tgttcccagg cgctgaacac cagccagatt
   100381 gatgtgcatt gtttccgcca tattttgcag attgttcgcg acaatgaagc agctgaatat
   100441 ctggagctga acgttgggga taactggcgg attagcgaag ggaagccaaa ccctgagcag
   100501 ccaatgcaga ttttaccggt gacaatgcaa gagacggttt acggcgaact gcactggcaa
   100561 aatagtcacg tttcatcatc agaaccgctg cttaacagtg tttcatcgat gctgggacgc
   100621 ggtttgtact ttaatcaggc gcagaagcat tttcagcagt tattgttgat ggaagaacgt
   100681 gcgaccatcg cccgcgaatt gcacgactcg ctggctcagg tgctttctta cttacgtatc
   100741 cagttgacgt tactgaagcg ttcgataccg gaagataacg ccaccgcaca aagtatcatg
   100801 gccgattttt cccaggcgtt gaatgatgct tatcggcagt tacgcgagct gttgactact
   100861 ttccgcctga cgctgcagca ggcggatctc ccctccgcgt tgagggaaat gctggatacg
   100921 ttacaaaatc aaaccagcgc taaactgacc ctcgactgcc gtctgccaac cctggcactg
   100981 gatgcgcaaa tgcaggtgca tttgttgcaa attattcgcg aagcggtgct gaatgcgatg
   101041 aagcacgcca acgccagcga aatcgccatc agttgcgtca ccgcgccgga cggcaatcac
   101101 acggtttata tccgtgataa cgggattggt atcggtgaac cgaaagaacc cgaaggtcat
   101161 tatggactga atatcatgcg cgaacgcgcg gaacggctgg gcgggacgct gactttttca
   101221 caaccttccg gcggcggcac gttagtgagt attagctttc gctccgcaga gggtgagggg
   101281 aaggggaagg ggaacacgcc ggctgaggtg taaataatta ccctcacacc ggccctctcc
   101341 ctttaaaggc gagagggaaa aacgtgccca tattgacctg taagcgagtg tgtcagcaat
   101401 ctcatgataa tttacattaa ctcctttttt tctccacgat tggctcgtac cttgccgcta
   101461 cagtgaagca agtcaagcct acaacaatac gcagaaacac gaggtcctct tttaatggcg
   101521 aatttcttta ttgatcgccc catttttgcc tgggtgctgg caatcctgtt gtgtctgaca
   101581 ggtaccctgg cgattttttc attgcccgtt gaacaatacc ccgatctcgc gccaccgaat
   101641 gtgcgagtga ccgctaacta tcccggcgca tcggcccaga cgctggaaaa caccgtgacc
   101701 caggttatcg agcaaaatat gaccggcctc gataatctga tgtatatgtc atctcagagc
   101761 agtggcaccg gtcaggcatc tgtcacttta agttttaaag caggcaccga tccggacgaa
   101821 gccgtgcagc aagtacaaaa ccagctgcaa tcagccatgc gaaagttacc gcaggcggtg
   101881 caaaatcagg gcgtgacggt gcgtaaaacc ggcgatacca acattctgac cattgccttc
   101941 gtctctaccg atggctcgat ggataaacag gatattgctg attatgttgc cagtaatatt
   102001 caggacccgt taagccgcgt gaatggcgtc ggggatatcg atgcctatgg ttcgcaatat
   102061 tccatgcgta tctggctgga cccggcgaaa ctcaacagtt tccagatgac ggctaaagat
   102121 gtcactgatg ccattgagtc acagaacgcg cagattgcgg ttgggcaact tggtggtaca
   102181 ccttccgtcg ataagcaggc gctcaacgcc gccattaacg cccagtcact gctgcaaaca
   102241 ccagaacagt tccgcgatat caccttgcgg gtgaatcagg acggctcaga ggtaaggctg
   102301 ggcgatgtcg ccaccgtcga aatgggggcg gagaaatacg attatcttag ccgcttcaat
   102361 ggtaagccag cctccgggtt gggggtaaaa ctggcctccg gcgcaaacga aatggcgaca
   102421 gcggagctgg tgctcaatcg tctcgacgag ctggcgcagt atttcccgca cggactggaa
   102481 tacaaggtgg cgtatgaaac cacctcgttt gttaaagcct ccattgaaga cgtggtgaaa
   102541 acgctgctgg aagctatcgc tctggttttc ctcgttatgt atctgttcct gcaaaacttc
   102601 cgcgccacgc tgatacccac tatcgccgtg ccggtggtgt tgatgggaac cttctccgta
   102661 ctttacgcct tcggttacag cgtcaacacc ttaaccatgt tcgcgatggt gctggcgatc
   102721 ggtctgctgg tggatgacgc catcgtggtg gtggaaaacg tcgaacgtat tatgagtgag
   102781 gaaggactca ctcctcgcga agccacacgt aaatcgatgg ggcagatcca gggggcactg
   102841 gtcgggattg cgatggtgct ttcggcggta tttgtaccaa tggccttctt cggcggcacc
   102901 accggtgcca tctatcgcca gttctctatt accattgttg cggcgatggt gctgtcagta
   102961 ctggtagcga tgatcctcac tccggctctg tgtgccacgc tacttaagcc actgaaaaaa
   103021 gctgagcatc acgggcaaaa aggttttttt gcctggttta accagatgtt taaccgcaac
   103081 gccgaacgct acgaaaaagg ggtggcgaaa attctccacc gtagcctgcg ctggattgtg
   103141 atttatgtcc tgctgcttgg cggcatggtg ttcctgttcc tgcgtttgcc gacgtcgttc
   103201 ttaccgctgg aagaccgggg catgtttact acctcggtac aattgcccag cggttcaacc
   103261 caacaacaga ccctgaaagt cgttgagcaa atcgagaaat attacttcat ccatgaaaaa
   103321 gacaacatca tgtcggtgtt tgccaccgtt ggttctggcc ctgggggtaa cgggcaaaac
   103381 gtggcgagaa tgtttatccg cctgaaagac tggagcgaac gcgacagtaa gaccggcacc
   103441 tcgtttgcca ttatcgagcg tgcaacgaag gcgtttaacc aaattaaaga agctcgcgtt
   103501 atcgccagca gcccaccagc aattagcggt cttggtagtt ctgcagattt tgatatggag
   103561 ttgcaggacc acgctggagc gggtcacgat gcgctgatgg cagcacgtaa tcagttgctg
   103621 gcgctggcgg cggaaaaccc ggagctaacc cgtgtgcgcc ataacggcct cgatgacagt
   103681 ccgcagttgc agattgatat cgaccagcgt aaagctcagg cgctgggcgt tgatatcgac
   103741 gatattaacg acacactgca aaccgcctgg ggttcgagct atgtgaatga ctttatggat
   103801 cgcggtcgcg tgaagaaagt ctatgtgcag gcagctgcgc cgtatcgcat gctgccagat
   103861 gacatcaatc tctggtatgt ccgaaataaa gatggcggca tggtgccctt ctctgctttc
   103921 gcgacctcac gctgggaaac aggctcgccg cgtctggaac gctataacag ttattctgcg
   103981 gttgagattg ttggggaagc cgcaccgggg gtcagtaccg gtacggcgat ggatattatg
   104041 gaatcgttag tgaagcagct gccaaacggc tttggtctgg agtggacggc gatgtcgtat
   104101 caggagcggc tttccggcac gcaggctccg gcgctgtacg ccatttcctt gctggtggta
   104161 ttcctgtgtc tggcggcatt gtatgaaagc tggtcggtac cgttctcggt aatgctggtc
   104221 gtgccgctgg gggtaatcgg cgcgctgctg gcaacctgga tgcgcgggct ggaaaacgac
   104281 gtttacttcc aggtgggcct gttaacggtc attggtttat cggcgaaaaa cgccatcctg
   104341 atcgtcgagt ttgctaacga gatgaaccaa aaaggccacg acctgtttga agcgacgctc
   104401 cacgcctgcc gtcagcgttt acgcccgatt ctgatgacct cgctggcatt tatcttcggc
   104461 gtattgccaa tggcaaccag cacgggtgcc ggttccggtg gtcagcatgc ggtgggtact
   104521 ggcgtaatgg gcgggatgat ttcagctact attctggcta tttacttcgt gccgctgttc
   104581 tttgtgctgg tgcgccgccg cttcccgctg aagccgcgcc cggaataagc aataaaaaag
   104641 gcgacatgcc aatgtgtcgc ctttttcaac tttccgatta agaacctgct cagcgggttc
   104701 ttgctgttta tactttgtct caggaattac ttacgaagca taacttcgat aaagtctttc
   104761 cagttcccca gttcacgttc aatcataaca acctctctta taattatggg tattctacgg
   104821 aaaaaatata ccgtggtgaa gctaatttac tcgattgctg cgatgactac ctccggggga
   104881 caaaccttat gtaaatacta tggtcctgcg gtgataattt gtatgtgata cacagcaaca
   104941 tttcgagata ttcatacggc atctaatact tatttaattc tggttaaaat acagacagat
   105001 aacaagatga atattcttaa tgtttacgtt aaaaatgttt aatattattt aatagttgtt
   105061 aatttgaata cttcgataat gttatatttt ctgataatca tttgcaggca aaatgttttc
   105121 acccttaaat gagtatttat tctcataaat cgaaaaagga ttcattatgg ttacacttta
   105181 cggcatcaaa aattgtgaca ccattaaaaa ggctcgccgt tggctggaag ccaataacat
   105241 cgactatcgt tttcatgatt accgcgtcga tgggctggac agcgaattat tgaacggttt
   105301 tatcaacgaa ttaggctggg aagcgttact caacacccgt ggtacaacct ggcgtaaact
   105361 ggacgaaact acccgcaata aaatcaccga tgcggcctct gcggcggcat taatgactga
   105421 aatgcctgca attatcaaac gtccattgct ctacgcgcct ggtaagccta tgctgctggg
   105481 tttcagtgat tccagttatc agcaattttt ccatgaggtg tagtctatgt cgtgcccggt
   105541 tattgagctg acacaacagc ttattcgccg cccttccctg agtcctgatg acgcaggatg
   105601 tcaggctttg ttgattgaac gtttgcaggc gatcggtttt accgttgaac gcatggattt
   105661 tgccgatacg cagaattttt gggcatggcg tgggcaaggt gaaacgttag cctttgccgg
   105721 gcataccgac gtggtgccgc ctggcgacgc cgatcgttgg atcaatccgc cgtttgaacc
   105781 caccattcgt gacggcatgt tattcgggcg cggtgcggca gatatgaaag gctcgctggc
   105841 ggcgatggtg gtagcagcag aacgttttgt cgcacaacat cccaaccata cggggcgact
   105901 ggcatttctg atcacttctg atgaagaagc cagtgcccac aacggtacgg taaaagtcgt
   105961 cgaggcgtta atggcacgta atgagcgtct cgattactgc ctggtcggcg aaccgtcgag
   106021 tatcgaagtg gtaggtgatg tggtgaaaaa tggtcgtcgc ggatcgttaa cctgcaacct
   106081 caccattcat ggcgttcagg ggcatgttgc ctacccacat ctggctgaca atccggtaca
   106141 tcgcgcagca cctttcctta atgaattagt ggctattgag tgggatcagg gcaatgaatt
   106201 cttcccggcg accagtatgc agattgccaa tattcaggcg ggaacgggca gtaacaatgt
   106261 tattccgggt gaactgtttg tgcagtttaa cttccgcttc agcaccgaac tgactgatga
   106321 gatgatcaaa gcgcaggtac ttgccctgct tgaaaaacat caactgcgct atacggtgga
   106381 ttggtggctt tccgggcagc catttttgac cgcgcgcggt aaactggtgg atgcggtcgt
   106441 taacgcggtt gagcactata atgaaattaa accgcagcta ctgaccacag gcggaacgtc
   106501 cgacgggcgc tttattgctc gcatgggggc gcaggtggtg gaactcgggc cggtcaatgc
   106561 cactattcat aaaattaatg aatgtgtgaa cgctgccgac ctgcagctac ttgcccgtat
   106621 gtatcaacgt atcatagaac agctcgtcgc gtgatgagtg gttctgcaag aggaaataag
   106681 catggactgg ctggctaaat attggtggat tctggtgatt gtctttttgg tcggcgtcct
   106741 gctgaacgtg attaaagatc tcaagcgcgt cgaccataag aaatttctcg ccaacaagcc
   106801 ggagcttccc ccgcatcgtg atttcaacga taagtgggac gatgacgacg actggccgaa
   106861 aaaggatcaa ccgaagaagt aagctgagtg ctcaaccgta ggccggataa ggcgttaacg
   106921 ccgcatccgg caatggtgaa cgatgcctga tgcgacgctc acgcgtctta tcaggccagg
   106981 taatctgtct tttacatcat ttcaatcaca tcatcatcgc caggcttacc gccacttagc
   107041 gcttggtcga aataatgttt cggaatggta taacgcaagt gatccagggc aaattgcata
   107101 ctgcgattgt cgatcgcatg gcctaaatcc tccacgatat ccagcgtcac gtcaccaccg
   107161 gcactgatta acgcttcctg cgccgcaact gcatgcgcca gatcgataac cggatcttca
   107221 ccaccatgaa tcaagtgaat tgtcgtggct gtagacgcgg tctccggcag gctcgcatag
   107281 cgtccgttaa aggcaatgac gcgcgaagca aggcccggtt cggctttaat gctctccagc
   107341 gccataatcg cgccttgtga gaaaccaatt agcgccgtgg cgttagcccc tactccgctc
   107401 tgtttctgcc agtagcgtac cgtttcaata aacgtcggca tgatggcatc cacacgcgcc
   107461 tggcgattat cttccgtaat accctgcact gaaaaccact gacgccccgc cggattaccg
   107521 ctcggctccg cgccaccgac actcaccacc agcgcatcag gaaacagcgg tgcaaaccag
   107581 ctgcctattt cccccattgc caccgggtta tccccgacac cgtggaaaag cagtaacaac
   107641 tgttgtgcag gtttatccgg gctttgaaca acaaaatggt catgtttcat ggcgaactcc
   107701 ttaactgatg tcattaattt tacgccgctg actcacaatg accatgggag aaaattgaag
   107761 gagtcagtga aaaaattgcc attgcgttat gcgatcgcgc agacgctcag tgcgaacctc
   107821 atttagtgcg aacagcgcct gcgcggcctc ttcccgctgg cgaaccagta acatcttgcg
   107881 gcctgaaagt ttaagtgtgg tacataactg cgcgtcactg gcgtttttct gtaaacaccc
   107941 acgcagcgcc ggtaatgcca gttcactggt ttgtagcaga cgcaataagc aacctaacga
   108001 tgttaatagc ggacgatgag cgaaagcaaa accggccagt tcaagccagt cgtcgtcaga
   108061 aaggatggca tcgtttagtg gatcaacagg aagcatttcg ccattccact gcgcgagagc
   108121 ttgcgcatcg cgacgtaaac ggtaatgctc acgttcagcc agctgtttac ccgcatcact
   108181 catcggtaac agcgccatcg ccgtatagca accgctgctg gcttcccgat gattacccat
   108241 ccgcaccagc acaaaaccgc agcgttgcca gaaacgccat aactccccag tgtaaccaaa
   108301 actcaccgaa agatagtcga ggtcatgcgt atattgcaaa gctccagcaa taagttgccg
   108361 ccctgtgcct tcccgctgac gagccggatg aactgctatc cggctaaccc gccttccgcg
   108421 caatgtcgcc gccagtggat tgctgccgtg cgccgccagc gactgggcca ccagattacc
   108481 ccgcgggcga cgaaaacctg cccataccgc ctgactgagt tcttgagata atccgccctc
   108541 atccaccagc cacaacgccc cggcaatctc gttttcgcca gccgcctgta aaaaatgttg
   108601 ccctagtgca tccatcatcc ggcgtaaatc cagcggcgag gtccggtagt gcgcaccaga
   108661 caataactga taaaccttta acggcgtttc tggctcgctt cgccataacg tttgttcaaa
   108721 tgcggaaatg acgatattgc cttgtggtgt atgggtgaag ttttcatcgt taaaaaccag
   108781 tgcctcacta accatttttt ccagcgggca tccctgtgcc cagcggatcg gctgttgcag
   108841 ttcaaaacgg tgtaaatgcg gaaagcgagc gcaaaatttc agcaaaaaac cacgtccggt
   108901 accttcgtag ccctgcaccg tagtggttaa caacgttcga ggaaaacgcg ataccagttg
   108961 atgcaacaac ggcgcaggta tggctgcggc ttcgtcgacc accagccagt cggcttgctc
   109021 atcgctggct aacaaggcat ccggtgcaat aaagcgaaac ttctcgcccg caaattgtgc
   109081 cagtacatcc gttgccgctt ttgcgggcgc ggtgacaatc gcactgcccg caatacgaga
   109141 aatgagttgc cctgccaacg ccgacttacc gcgcccacgc gcagccgtta ccgctgccac
   109201 gcccggcggc atggtcagta gctgctgtaa gagttgctgt tgttctggtt gtggtgcgcc
   109261 ggtcgcgggg tgccagtcag tacggggagt aaaatgcgcc aacgagaacg gctggttttg
   109321 ccgccagagg atagcgtcgt tatccgccgt aagtacgcgt ttgagatgct ggacaaaatg
   109381 cggcgtcgca atagggtcag ggcaatcact ccagcgcagc gagtcggcat caggttggtt
   109441 ttcccactct tcccatacag ggagtaacaa aaccagccag cttcccgctt tcaacgttcc
   109501 gctaagtgct gcaaaggcag cggcatcaaa gccgtggcgg gcgtcgaata ccgcatgccg
   109561 gaactcgcgc ccaagtaaag tttgtagcgc cgagggtgaa cagtggtttt cagcatctag
   109621 ctgcggcgaa atccacagcc agtcgccagg taaggcatca cgcaacttaa gagcatgatc
   109681 aaaacaccaa ccctcttctc cgctcaacac cagcaagcgg cagatccctt cacgtttcat
   109741 ttgcgctgtt aatgtgtaaa gcgcagtcag ttcagccatc cctgccccgg aaattaaatg
   109801 cttttaccaa aagtattgca ttgtgccgga tcgccgctgt cgaaaccacg tttaaaccag
   109861 ctgcagcgtt gctgagaagt gccatgtgtg aaactgtctg gtactactcg cccctgactt
   109921 tgctgttgta aacggtcatc gccgatggcc tgcgccgcat tcagcgcctc ttccagatcg
   109981 ccagtttcca gaacgccttg ctgctgcata ctatgccccc agacaccggc aaaacagtcg
   110041 gcctggagtt ccatacgcac agataagcgg tttacttccg cctgcgtcgc gttttgttgc
   110101 agttgacgaa ctttcggctc gatgcctaac agtttctgca catgatgacc gacttcatgg
   110161 gcgataacgt acccctgggc aaaatcgcca tccgcgccaa gtttgtcttt catgtcatca
   110221 tagaaggaga gatcgatata aactgtgcca tccgccgggc aatagaacgg ccccataatg
   110281 gactggcccg ccccgcagcc ggtgcgcgtc attccacggt acatgaccag cttcggttgc
   110341 tgataggtct tacccatctt ttcgaactgt tgtccccagg tgtcttccgt ggttgccaga
   110401 atcaccgagg tgaattttgc ggcttcgtct tcattagggc taattgaccg cgttgattgt
   110461 tgttgagaaa ccggctgccc ggtcatcaac ccggttaaat caacgccata gtagcctgca
   110521 accagcacga ctatcagtaa aatcagcccg cctttaccgc tcggcagacg gaaaccggga
   110581 ccgcccattg acggaccgcc agagctgttg cgcctgtctt caacattgtc actttcacgt
   110641 cgcccttgcc aacgcataga tacctcaaca atatattcat tatagaaatg atcgtaggtg
   110701 gttcagagga agattaccac aggaaagcgg gatgaaatgg cggaggaatg agcgttcaat
   110761 gcatatattc cgagagcggt tgtcttgccg tgccagctgc acggcaagat gatgaacaga
   110821 aaaatcagtc cagctgtaca cccaggcggc gggcgacggc ttcataggct tcgatcaggc
   110881 caccgaggct ctggcggaaa cggtctttgt ccattttctc cagtgtttct ttgtcccaca
   110941 ggcggctgcc gtccggggag aactcatcgc ccagtaccac ttcacctttg tacagaccaa
   111001 actccagctt gaagtcgacc agaatcagac cagcatcatc gaacagtttt ttcagcacgt
   111061 cgttcgcttt gtaggtcagc tctttcatac gtgccaggtt ctctttgctc acccagccaa
   111121 aggtttcgca gtaagattcg ttgaccatcg gatcgtgcat ggcatcgttt ttcaggaaca
   111181 gatcgaacag cggcgggttc agctcaatac cttcttcgat tccaagacgt ttcaccagag
   111241 agccagcagc acggttacgc acgacacact caaccggcac catatccagc tttttcacca
   111301 gacattcggt atcggagagc agacgctcca tttgagtcgg gatacccgct tcagccagtt
   111361 tgctcataat gaagtagttg aacttgttgt tcaccatacc tttgcgatca aactgctcaa
   111421 tgcgcgcgcc atcccctgct gacgtatcat tgcggaattc gagcaccaac aggtccgggt
   111481 tttccgtgct gtatacggtt ttcgctttac cacgatacaa ctcagcttgc ttttgcatct
   111541 ttatcactcc tgggtgtgaa ttaacgtttt aaaatctttt gctgtctggt gtgccggatg
   111601 ttttttcgga tgcggcgtga acgccttatc cgaactacgt ccgacaaaca aatttcgtgc
   111661 gaattaccgc taaaatcgcg ttttcctgcc gacgcacacg tttgcgtatc atatcagaaa
   111721 aaagggccgg atgattccag ccctgtattt ttacttgcta aacgcagcct ggaagacagc
   111781 taccagcgcg tcgttctgac tctgagtcag agtatgacct ttcggatcga tgaactgtag
   111841 gctgctgcgg ttatctaaat cgccaacctg cagtttatag tcaccggatg ccaggcctgg
   111901 atcgctcgcg cccagttcct gccagtcgct gtcagacagc ggcttataag ttacggccat
   111961 gttgccctgc gaacgggtgc tgtcggtcac tttcatgccc actttttcca gcgacgctgg
   112021 cagacgttgc caaaccacat tgaacggccc gcgtacgacc agcattggta aaccggtgtc
   112081 atcagctgca ctttgtacgt ccatagtggt ggaggcacga ttttgcgcag cgttcgcggc
   112141 gtcagtggca gatttatcca gaccggcgga aataacgttc atcatctccg tgctgtaacg
   112201 ctgcatggaa gccgcgtctg caaccggttt gcccgcctgt tccaggttca gcagtttaac
   112261 cgtaaccgcc tgctgataac cctgcggctt aacagagatt tgataacgac cacgatactg
   112321 ctcgtcttcg tccagacggt tccattgtac ccaatcggtg gtcagtgtct gaccagcatc
   112381 atcacgttgg gtgatggtgt agtttttcgc ctgcagcacg ctaaccacct gcggccacag
   112441 agtattgcca cgaccatttt ccaccagcaa tgaagcggta tcgcccgtga actgggtacg
   112501 cgcgccagaa accagtgcca gcggctgggc tggtggacga atgtccagcg ccttaccgac
   112561 agcaccacta ccgttggtca ccgggattgc ataatcaccg gaggtcaccg gcaaaatcat
   112621 tccagccggg gcatgaagct ccgcaagcgg tgccgcttcc aggtaggctt catcaccact
   112681 gacctgacgc ttatagcgtg agtcagaact acaggcagcg agtaataaaa caagcgaaac
   112741 acccgcaacc tttgccaggc gcgacttttg aacagagtaa gccatcaaat ctccctaaac
   112801 tttacagcaa accggcatgc ttaagcgccg ctctgaccgt ctcacgacca ctgtcggtga
   112861 ttggtgtcat tggcaggcgc agcgtatcgg tcgccacaag acccagttcc ttacatgccc
   112921 atttcaccgg gattggattg ggttcgacaa atagtttgtt gtgtaatggc atcagacgct
   112981 gattaataac gcgtgcctcg gcaaaatgcc cttctgctgc cagtttgcac atctgggcca
   113041 tatcacgcgc tgcgacgtta gccgtaacgg aaataacccc atgaccgccc aattgcatga
   113101 agtccagcgc gctcgcatca tcgccgctca gcagaacaaa atcatctgaa accagctctt
   113161 tgatctggtt tacacgcgtt aagttccctg ttgcctcttt gattccgata atatttttta
   113221 ctttcgccag acggcccacc gtttccggga gcagatcgca gccagtacgg gacggcacat
   113281 tatacagaat ttgcggcagg tcagtatgct cagcgatggc tttgaaatgc tgatacaaac
   113341 cttcttgcga cggacgattg tagtaagggg ttaccgtcag gcagccgacg ataccactgt
   113401 cattgaagcg ctgcgtcagg ctaatggctt ccgcagtagc gttagcgccg gtcccggcaa
   113461 ttaccggaat gcgcccatca gccagatcca gcgtcatcat caccacatca gcatgttcgt
   113521 catgatttaa ggtagcggac tcgccagtgg tgccaacaga aacgatcgcc gaagtaccgc
   113581 tggcgacatg ataatcaatc agttttttca agctagcccg acagacatta cctttttcat
   113641 ccatcggagt aacaatcgcg acaatacttc ccgtgaacat gggccatcct ctgtgcaaac
   113701 aagtgcctca atggtacgtt tggtatggca ttaaaagcaa gcagacagaa ccgttctgat
   113761 tgttgtatgc atgatttttt tatgctttcc ttaagaacaa ctcacccctt aaaggaataa
   113821 ccagtttgac actgtcatcg caacattatc tggtgatcac tgcgttgggt gccgatcgcc
   113881 ctggaattgt gaacaccatc acccgtcatg tcagtagttg cggctgtaat attgaagaca
   113941 gtcgcctggc gatgctggga gaagagttca cgtttatcat gctgctttcc ggttcatgga
   114001 atgccatcac tctgattgaa tcaacgttac cgttgaaagg tgccgaactg gatcttttaa
   114061 tcgtgatgaa gcgcacgacg gcgcgtccgc gtccgccaat gccagcatct gtctgggttc
   114121 aggtcgatgt tgcagactcc ccgcatttaa ttgaacgctt cacagcactt ttcgacgcgc
   114181 atcatatgaa cattgcggag ctggtgtcgc gcacacaacc tgctgaaaat gaacgggctg
   114241 cgcagttgca tattcagata accgcccaca gccccgcatc tgcggacgca gcaaatattg
   114301 agcaagcgtt caaagcccta tgtacagaac tcaatgcaca aggcagtatt aacgtcgtca
   114361 attattccca acatgatgaa caggatggag ttaagtaatg aatccactga aagccggtga
   114421 tatcgcaccg aaatttagct tgccggatca agacggagaa caagttaatt tgaccgactt
   114481 ccagggacag cgtgttctgg tttatttcta cccgaaagcc atgacccccg gctgtaccgt
   114541 acaggcctgc ggcttacgcg ataacatgga tgagttgaaa aaagcgggcg ttgatgtgct
   114601 gggtatcagc accgataaac ccgaaaaact ctcccgtttt gcggaaaaag agctgcttaa
   114661 ctttacgctc ctgtctgatg aggaccacca ggtgtgcgaa caattcggcg tctggggtga
   114721 aaagtccttc atgggcaaaa cctacgatgg tattcatcgc atcagcttcc tgattgacgc
   114781 tgatggcaaa atcgaacatg tctttgacga tttcaaaacc agcaatcacc acgacgttgt
   114841 gctgaactgg ctgaaagaac acgcctgatt actttgctcc attccgtgct ggctgcgctt
   114901 gcggccagca tacctcactt ctcgtgatca agatcacatt ctcgctttcc cctgcgacac
   114961 gggtgtcgaa tccatttttt gctgaacgtt aatgaccatc atttttgtac cgttcagaat
   115021 ccagttaata cataacttat tgaatagatt gagttaatca gaatggcacc ctttatgcaa
   115081 tatgaaatgc aatgtttcat atcattttca aggagccgac atgaaccgct ttgtggtggc
   115141 cgaaccactg tggtgtacag gatgtaatac ctgtctcgct gcctgttcgg acgtgcataa
   115201 aacgcaaggt ttacagcaac acccgcgcct ggccctggcg aagacgtcaa caatcactgc
   115261 ccctgtcgtg tgtcatcact gtgaggaagc cccttgcctg caggtctgcc cggtcaatgc
   115321 catctctcag agggatgatg cgatccaact caacgaaagc ctctgtattg gctgcaagct
   115381 ttgcgccgtg gtctgcccat ttggcgcaat cagcgcttca ggaagccgtc cggtgaatgc
   115441 ccatgcgcaa tatgtttttc aggctgaagg ctcactcaaa gacggcgaag aaaacgtgcc
   115501 gccccaacat gcgttgctgc gctgggaacc gggtgtccag accgtcgcgg tgaaatgcga
   115561 cctgtgtgat ttcttgccag aaggtccggc ctgcgttcgc gcttgcccga atcaggcgtt
   115621 acggctgatc accgacgata gcctgcaacg tcagatgaaa gaaaaacagc gccttgccgc
   115681 aagctggttt gccaatggcg gggaggatcc cctttccctc actcaggagc aacactaatg
   115741 gatgccctgc aattattaac ctggtcgctg atcctctatc tgtttgccag tctggcttcg
   115801 ctgtttttac tcggtctgga cagactggct attaagcttt ccggcatcac atcgctggtg
   115861 ggcggcgtga ttggcatcat cagcggaatt acgcaattac atgctggtgt aactttagtc
   115921 gcccattttg ccaccccttt tgactttgcc gatttaaccc tgcgaatgga tagcctctcg
   115981 gcatttatgg tgctggttat ctccttgctg gtggtggttt gttcgctcta ttcattgact
   116041 tatatgcgcg aatacgaggg caaaggcgcg gcggcgatgg gcttctttat gaatcttttc
   116101 atcgcatcga tggttgccct gctggtgatg gacaacgctt tttggttcat cgtgctgttt
   116161 gaaatgatgt cgctgtcttc ctggtttctg gtcattgcca ggcaggataa aacgtcgatc
   116221 aacgctggca tgctctactt ttttatcgcc cacgccggat cggtgctgat tatgatcgcc
   116281 ttcttgctga tggggcgcga aagcggcagc ctcgattttg ccagtttcgc acgctttcac
   116341 tttctccggg gctggcgtcg gcggtgtttc tgctggcctt tttcggtttt ggcgcgaaag
   116401 ccgggatgat gccgttgcac agctggttgc cgcgcgctca ccctgccgca ccatcgcacg
   116461 cttcggcgtt gatgtctggc gtaatggtca aaatcggtat tttcggcatc ctgaaagtag
   116521 cgatggatct gctggcgcaa acgggtttgc ctctgtggtg gggcattctg gtgatggcga
   116581 tcggcgcaat ctccgcgctc ctgggcgtgc tatatgcgct ggcggaacag gatatcaaac
   116641 ggctgctggc ctggagtacc gtcgaaaacg tcggcattat tttgctggca gtcggtgtgg
   116701 cgatggacgg tctgtcactg cacgacccgc tgctcaccgt ggttggactg ctcggcgcac
   116761 tgtttcatct gctcaaccat gcgctgttca aagggctgct atttctcggc gcggacgcga
   116821 ttatttcgcg tttgcatacc cacgacatgg aaaaaatggg ggcactggcg aaacggatgc
   116881 cgtggacagc cgcagcatgc ctgattggtt gcctcgcgat atcagccatt cctccgctga
   116941 atggttttat cagcgaatgg tacacctggc agtcgctgtt ctcactaagt cgtgtggaag
   117001 ccgtagcgct acaacttgcg ggtcctattg ctatggtaat gctggcagtc actggtgggc
   117061 tggcagtaat gtgcttcgtc aaaatgtacg gtattacttt ctgtggtgcg ccgcgcagta
   117121 cacacgctga agaggcacag gaagtgccaa atacgatgat cgtcgccatg ctactgctcg
   117181 cggcactctg cgtattaatt gcgcttagtg ccagttggct ggcaccgaag ataatgcata
   117241 ttgcccatgc gtttaccaat acccctcccg tcactgtcgc cagcggaata gcacttgtac
   117301 ccggcacgtt tcatacacgg gtcactcctt cattactgtt gctgttacta ctggcgatgc
   117361 ctttgctgcc aggcctttac tggctgtggt gtcgttcgcg ccgcgcagcg tttcgtcgca
   117421 caggagatgc ctgggcatgc ggctacggct gggaaaacgc gatggctccg tcaggcaatg
   117481 gcgtgatgca gccgctgcgt gtggtcttct ctgcgttatt tcgtctacga caacagctcg
   117541 accctacgct gaggctaaat aaaggtcttg cgcacgtcac cgccagggct cagagcacag
   117601 aacccttctg ggatgagcgg gtgatccgcc ccatcgtgag cgccacccaa cggctggtca
   117661 aagaaataca gcatctgcaa agcggcgact ttcgtctcta ttgcctgtat gtggtcgccg
   117721 cactggttgt gctgctaatc gctattgccg tctaaggaaa tcaccatgag acaaactctt
   117781 tgcgacggat atctggtcat ttttgcgtta gcacaggccg tgattctgct gatgctaacc
   117841 ccacttttta cgggtatttc ccggcagata cgcgcgcgta tgcactcccg ccgcgggccg
   117901 gggatctggc aggattatcg cgatatccac aaactgttta aacgccagga agttgcgccg
   117961 acatcttcag gtctgatgtt ccgcctgatg ccgtgggtat taatcagcag catgctggtg
   118021 ctggcgatgg ccttaccact atttattacc gtttcccctt ttgcgggcgg cggcgatctg
   118081 atcaccctta tctatcttct tgccctgttt cgttttttct ttgctctttc cgggctggat
   118141 accggaagtc cgtttgcggg agtcggtgcc agtcgcgagt tgacgctcgg cattctggtc
   118201 gaaccaatgc ttattctctc actgctggta ttggcgctga tagcaggttc cacgcatatc
   118261 gagatgatca gcaatacgct ggcgatgggc tggaactcgc cgctaaccac cgtactggcg
   118321 ttactggcct gtggttttgc ctgcttcatt gagatgggaa aaattccctt tgatgttgct
   118381 gaagcagaac aggaattaca ggaaggcccg ctgaccgaat attccggtgc cgggctggcg
   118441 ctagcgaaat gggggcaggg gctgaaacag gtcgtgatgg cagcactgtt tgtggccctg
   118501 tttctgccct ttgggcgcgc gcaagaactt tctctcgcct gcctgctgac ttcacttgtc
   118561 gttacgctgc tcaaggtttt gctgattttt gtactggcct caatcgcaga aaacacgctg
   118621 gcacgcgggc gttttttact cattcaccat gtgacctggc ttggcttggc ttcagccttg
   118681 ctgcgcttgc atgggtcttc tggttaaccg gtctgtaagg agcactgacg gaatatggaa
   118741 aatcttgctc tgacgacgtt aatgctgcct tttatcggcg cactggtcgt ttcgttttcg
   118801 ccacaacgtc gggccgccga atggggggtt ttgttcgccg cgctgaccac gctgtgcatg
   118861 ttgtcgctga tctccgcgtt ttatcaggcc gataaagttg ccgtcacgtt gacgttggtc
   118921 aacgtggggg atgtggcatt gtttggcctg gtcattgatc gcgtgagtac gctgattctg
   118981 tttgtggtgg tgtttctcgg tttgctggtc acgatctact ccacgggtta tctgacggat
   119041 aaaaatcgcg aacacccgca taacggcacg aatcgttatt acgcattttt gctggtgttt
   119101 atcggcgcga tggcgggact ggtactctcc tcgacgctgc tcggtcagtt gttgtttttt
   119161 gaaattacgg gcggctgctc ctgggcgttg atcagttatt accagagcga taaagcgcag
   119221 cgttcagcac taaaagcgtt gcttatcacc catatcggtt cgttggggtt gtatcttgcc
   119281 gccgccacgc tgtttttgca gaccggaacg tttgtgctta gcgcgatgag cgagttacat
   119341 ggcgacgcac gttatctggt ttatggcggc atcctgtttg ccgcgtgggg gaaatcggcc
   119401 cagctaccga tgcaagcgtg gctaccggac gcaatggaag cgccaacacc gatcagcgac
   119461 tatctccacg ccgcatcgat ggtgaaagtg ggcgtttaca tttttgcccg cgccattatc
   119521 gacggcggca atatcccgca tgtgattggc ggcgttggca tggtcatggc actggtcacc
   119581 attctttatg gctttctgat gtatttgcca cagcaggata tgaagcggtt gctggcctgg
   119641 tcgaccatca ctcaacttgg ctggatgttc ttcggcttgt cgctctccat cttcggctcg
   119701 cggctggcgc tggagggtag catcgcctac atcgtcaacc acgcgttcgc taaaagcctg
   119761 tttttccttg tagcaggtgc gctgagttac agctgcggca cgcgcttgtt gccgcgtctg
   119821 cgtggcgtat tgcacaccct gccgttgcca ggcgtgggtt tctgcgtggc agcgctggca
   119881 attaccggtg tgccgccgtt caacggcttc ttcagtaaat tcccgctgtt tgccgccggt
   119941 tttgcgttgt cagtggagta ctggatcctg ctgcccgcca tgattctgct gatgattgaa
   120001 tcggtcgcca gtttcgcctg gtttattcgc tggtttggtc gcgtcgtgcc gggcaaaccg
   120061 agcgaggccg tcgccgatgc cgcaccgctg ccagggtcaa tgcgcctggt gttgattgta
   120121 ctgattgtga tgtcgctgat ttccagcgta atcgccgcga cctggttgca gtaaggagat
   120181 gatgaatgac cggttctatg atcgtaaata atctggcggg actgatgatg ctgacatcgc
   120241 tgtttgtgat tagcgtcaaa agctatcgcc tgtcatgcgg attttacgcc tgccagtcgc
   120301 tggtgctggt gtctattttc gccactctct cgtgcctgtt cgccgcagag caactgctga
   120361 tctggtccgc cagcgccttt atcaccaaag tgctgctggt accgttaatc atgacttacg
   120421 ctgcacgaaa tattccccag aacatcccgg aaaaagcgtt attcggtccg gcaatgatgg
   120481 cactgctcgc ggcgttaatt gtcctgcttt gcgcatttgt cgttcagccc gtgaagctac
   120541 cgatggctac cgggctgaaa ccggcgctgg cggtagcgtt aggtcatttt ctgcttggcc
   120601 tgctgtgcat tgtcagccag cgcaatatcc tgcggcaaat ttttggttac tgcctgatgg
   120661 aaaacggctc ccatctggtg ctggcgcttc ttgcctggcg agcaccggaa ctggtggaaa
   120721 taggtatcgc taccgacgcc atcttcgccg tcattgtgat ggtgttactg gcaagaaaaa
   120781 tatggcgtac ccacggcacg ctggacgtga acaacttgac cgcgctgaag ggataatgag
   120841 atgagttatt ctgtgatgtt cgctttactc ctgctcacgc cgctgctttt ttcgctgctc
   120901 tgttttgcct gccggaaacg ggggctctct gcgacttgta cggtgactgt attacatagc
   120961 ttagggatca cactgctgct gattctggca ctctgggtgg tccaaactgc cgctgatgca
   121021 ggagaaatat tcgctgcggg actgtggctt catattgatg gtctgggcgg tttgttcctc
   121081 gccattcttg gtgtgattgg ctttctcacc ggtgtttact cgattggcta catgcgtcat
   121141 gaagtggcac acggcgagct ttcacccgtt acactgtgcg attactacgg tttcttccat
   121201 ctgttttttt tcaccatgct gctggttgtt accagcaata acctgattgt gatgtgggcg
   121261 gcgatcgaag ccaccacctt aagctcggcg tttctggtag gcatttacgg tcagcgttca
   121321 tcgctggaat cagcatggaa gtacatcatt atttgtactg ttggtgtcgc ttttggtctg
   121381 ttcggtaccg tgctggtata cgccaacgcc gccagcgtta tgccgcaggc agaaatggcg
   121441 atattctgga gcgaggttct taagcaatcg tccttgcttg acccaacatt aatgctgttg
   121501 gcctttgtgt ttgtgctaat tggctttggt accaaaaccg ggctatttcc catgcacgcc
   121561 tggctgccgg atgctcacag tgaagcgccg agtccggtca gcgccctgct ctccgccgta
   121621 ttgctgaact gcgcgctgtt ggtgctgatt cgctattaca tcattatttg ccaggccatc
   121681 ggcagcgatt tccccaaccg gttgttgctc atcttcggca tgttgtcggt tgccgtggcg
   121741 gcatttttca ttctggtaca gcgggacatt aagcgtctgc tggcgtactc cagcgtggag
   121801 aacatggggc tggtcgcggt ggcgctaggc attggcgggc cgctgggaat ttttgccgcg
   121861 ctgctgcaca ccttaaacca cagtctggca aaaacgctgc tgttctgcgg ttccggcaat
   121921 gtactgctca agtacggcac gcgcgatctc aacgtcgtct gtgggatgct caaaatcatg
   121981 ccatttaccg ccgtgctgtt tggcggcggt gcgctggcgc tggcagggat gccgcccttc
   122041 aacatttttc ttagcgaatt tatgaccgtt accgcaggac tggcacgtaa tcacctgctg
   122101 attatcgtcc tgctgttatt gctgttaacg ctggtgctcg cgggcctggt acggatggct
   122161 gcgcgggtgt taatggcgaa accgccgcag gccgttaacc ggggtgatct cggctggttg
   122221 accacctcgc caatggtgat tctgctagtc atgatgctgg cgatgggaac gcatattcca
   122281 caacctgtca tcaggatcct ggcgggcgct tccactatag tcctctcagg gacgcacgac
   122341 ctgcctgcac aacgtagcac ctggcatgat tttttgcctt caggcaccgc atctgtttcg
   122401 gagaaacaca gtgaacgtta attcatcgtc aaatcgtggc gaagcgattc tcgccgccct
   122461 gaaaacgcag ttccccggcg cggtgctgga tgaagagcga caaacgcctg aacaggtcac
   122521 cattacggtg aaaatcaatc tgctgcctga cgttgtacat tatctttatt atcaacatga
   122581 tggctggctt ccagtcctgt ttggcaacga cgagcggaca cttaacggtc attacgcggt
   122641 ttattatgcc ctttcaatgg aaggggccga aaaatgctgg atcgtggtga aggcactggt
   122701 cgatgccgac agtcgggagt ttccgtcagt cacaccgcgc gtccctgccg cggtctgggg
   122761 cgagcgagaa attcgcgata tgtacgggct gattccggtt ggcctgccgg atcagcgtcg
   122821 cctggtgttg cccgatgact ggccggaaga tatgcatccg ctgcgcaaag atgcgatgga
   122881 ttatcgactg cgccctgaac cgacgacaga ttccgaaacg tatgcgttta tcaatgaggg
   122941 caacagcgat gcgcgggtga tccctgtcgg cccgctgcat atcacctcct atgaaccggg
   123001 tcacttccgc ttgtttgtgg atggcgagca aattgtcgat gctgattacc gcctgtttta
   123061 tgtccatcgc ggcatggaga aactggcaga aacgcggatg ggctacaacg aagtgacctt
   123121 cttatcggac cgcgtgtgtg ggatttgcgg ttttgcccac agtgtggcct ataccaactc
   123181 ggttgaaaat gcactgggga ttgaggtgcc gcaacgagca catactattc gctcgattct
   123241 gctggaagtc gagcggctac acagtcattt gctcaacctt ggcctctcct gccattttgt
   123301 tggttttgat accggcttta tgcaattttt ccgcgtgcgg gaaaagtcga tgacgatggc
   123361 ggaattgctg accgggtcgc gtaaaaccta cggtctgaat ctgattggtg gtgttcgccg
   123421 cgatattctc aaagagcaac gtctgcaaac gctgaaactg gtgcgcgaga tgcgctccga
   123481 cgtgtcggag ctggtagaga tgctgcttgc tacgccgaat atggaacaac gcactcaggg
   123541 cattggcatt ctcgaccgac aaatcgcccg tgattatagc cctgtagggc cgctgatccg
   123601 cgcagtggtt ttgcccgtga tttgcgcttt gatcacccct acgccgacta cggcaatatt
   123661 ccaaaaacac tgtttacctt taccggcggc gatgttttct cccgcgtgat ggtccgtgtc
   123721 aaagagacgt ttgattcgct ggcaatgctg gaatttgccc tcgacaacat gccggatacc
   123781 ccactgctga ccgaaggctt tagctataaa cctcacgcat tcgcgctggg ctttgttgaa
   123841 gcgccacgcg gtgaagacgt gcactggagc atgctcggtg ataaccaaaa attgttccgc
   123901 tggcgctgtc gtgccgccac ctacgccaac tggccggtgt tgcgttacat gctgcgcggc
   123961 aataccgttt ctgacgcacc gctgattatc ggtagccttg atccctgcta ctcctgtacc
   124021 gaccgtgtga cgctggtaga tgtgcgcaag cgccagtcaa aaaccgtgct gtataaagag
   124081 atcgaacgct acggcattga tcgtaaccgt tcgccgctga agtaaggaca gaagatgctg
   124141 aagttactga aaactattat gcgcgccgga accccgacgg tgaaatatcc cttcgcgcca
   124201 ctggaggtca gcccaggctt tcgcgggaaa ccggacctga tgcccagcca atgtattgcc
   124261 tgcggtgcct gcgcctgtgc ttgtccggca aatgcgctga ctatccagac cgacgaccag
   124321 caaaattcgc gcacctggca gctctatctg gggcgttgta tttactgcgg acgttgtgaa
   124381 gaagtgtgcc cgaccagagc catccagctt accaataact ttgaactgac cgtcacccat
   124441 aaagccgatc tctataccca cgcgacgttc catctacaac gttgcagccg ttgcgaacgc
   124501 ccgtttgccc cgcaaaaaac cgtcgcactg gctgctgaat tgttggcaca gcaacaaaat
   124561 gctccacaaa accgcgaaat gttatgggcg caagcgagcg tctgcccgga gtgcaaacaa
   124621 cgcgcgacgc tgatcaacga cgatacagat gtaccgctgg tggctaagga gcagctatga
   124681 gtccagtgct tacgcaatat gtcagccagc ccatcacgct ggacgagcaa acgcaaaaga
   124741 tgaagcggca tttgctacag gatatccgtc gctcggctta cgtttatcgc atcgattgcg
   124801 gcggctgcaa cgcctgtgaa atcgaaattt ttgctgccat cacccccgta ttcgacgcag
   124861 aacgttttgg cattaaggtt gtttcatcac cgcgtcacgc cgatattttg ttatttactg
   124921 gcgcagtcac ccgggcgatg cgtatgcctg cacttcgggc gtatgagtct gcccccgatc
   124981 ataaaatctg tgtttcctac ggcgcgtgcg gtgtcggcgg cggtattttc cacgatctct
   125041 acagcgtctg gggcggcagc gacaccattg tccccattga tgtttggatc cccggctgcc
   125101 cgccaacacc ggccgccacc attcacggtt tcgccgtggc gctcggtttg ctgcaacaga
   125161 agattcacgc ggtggattat cgcgatccca ccggggtgac tatgcaaccg ttgtggccgc
   125221 agatcccgcc atcacagcgt atcgccattg agcgagaagc gcggcggctg gcgggctatc
   125281 gtcaggggcg agaaatttgc gatcggctcc tgcgccattt aagcgacgat cctacaggaa
   125341 atcgggttaa cacctggttg cgcgatgccg acgatccacg tctcaatagt atcgttcagc
   125401 aactctttcg cgtactccgg gggttacatg actgaagagt gcggggaaat tgttttctgg
   125461 acgctgcgaa aaaagtttgt cgccagtagc gacgagatgc cggaacacag ctctcaggta
   125521 atgtattact cgctggctat cggtcatcac gttggcgtga ttgattgtct gaatgtcgcc
   125581 ttccgctgcc cactgacgga atacgaagat tggcttgcac tggtcgaaga ggagcaagcc
   125641 cgacgtaaga tgctgggggt gatgactttt ggtgagattg ttattgacgc cagccacacc
   125701 gccctgttga cccgggcatt cgcgccactg gcagatgacg cgacgtctgt gtggcaagcg
   125761 cgtagcattc aattcattca tctgttggat gaaattgtgc tggaaccggc tatctatctg
   125821 atggccagaa aaattgcgtg agaaggattt ctcattaata aggactgttg atggctatgt
   125881 cagacgaagc gatgtttgcc ccgccgcaag gaataacaat tgaagcggta aacggaatgc
   125941 tcgcggagcg gttagcgcag aaacacggca aggcgtcttt attacgcgcc ttcatcccgc
   126001 tgccgccgcc gttcagcccg gtacaactta ttgaactgca tgttctcaaa agcaacttct
   126061 attaccgcta ccatgatgat ggcagcgatg tgacggcaac aacagagtat cagggcgaga
   126121 tggtcgatta ttcgcgtcac gccgtccttc tcggcagtag ttgaatggcg gagctacgct
   126181 ttattcgtac ccacggcagc cgttttactc cccaggattg cacactgttt aactggctgg
   126241 cacgcattat caccccggtt ctgcaatcat ggctcaatga tgaagaacag caggtggcgc
   126301 tgcatttgct ggagaaagat cgcgatcatc atcgggtact ggtggatatc actaatgcag
   126361 tgctgtcaca tcttgatctc gacgatctga tcgctgacgt cgctcgtgag atccatcatt
   126421 ttttcggtct ggcttcagtc agtatggtac tgggcgatca tcgaaagaac gagaagttca
   126481 gcctgtggtg cagcgatctt tctgcctcac attgtgcgtg tctgccacac aatatgcctg
   126541 gcgacagtgt attgctgaca caaacgctac aaacccgaca accgaccttg acgcaccgtg
   126601 cagatgatct gtttctctgg caacgcgacc cgttattact cttacttgca tctaacggct
   126661 gcgaatctgc gctccttata ccgcttacct ttggcaacca tacaccgggt gcattgttgc
   126721 tggcgcatac ctcttccact ctctttagtg aggaaaactg ccagctacta caacacatag
   126781 ccgatcgcat cgctattgcc gttggcaatg ccgatgcctg ccgtagaatg accgatttgc
   126841 aggaaagttt gcagcaagaa aaccaccagc ttagcgagca gctcctttcg aatctgggcg
   126901 tcggtgacat tatctatcaa agccaggcaa tggaagacct gctccagcag gtagatattg
   126961 tggcgaagag cgacagtacg gtgttgattt gcggtgaaac cggaaccggc aaagaggtga
   127021 tcgccagagc gatccatcaa cttagcccgc gacacgacaa gccgctggtc aaaatcaact
   127081 gcgctgccat ccccgccagt cttctggaaa gtgagttatt cggtcatgac aaaggggcgt
   127141 ttactggtgc gattaatacc catcgtggtc gttttgaaat tgccgatggc ggcacgttgt
   127201 ttctcgatga aattggcgat ctgccgttag aacttcagcc taaactgctg cgcgtattgc
   127261 aggaacggga gattgagcgt ctcggcggga gtagaacgat cccggtaaat gtcagagtca
   127321 ttgccgccac caaccgtgat ttgtggcaaa tggttgaaga tcgccagttt cgcagcgatc
   127381 tcttttatcg cctgaatgtc ttcccactgg aattgcctcc gctgcgcgac cgtccggaag
   127441 atatccctct tttagcaaag catttcacgc aaaaaatggc gcgccatatg aatcgttcaa
   127501 ttgacgccat cccgaccgag gcgctacgcc agttgatgtc gtgggactgg ccgggcaacg
   127561 tgcgcgagct ggaaaacgtg attgagcgag cggtactgct gactcgcggt aacagtctga
   127621 atttacatct taatgtccga caaagccgtt tactgccgac gctaaatgaa gattcagcgc
   127681 ttcgcagttc aatggcgcag ttgctgcacc cgacgacgcc agagaatgac gaagaagaac
   127741 gtcagcgcat tgttcaggta ttgcgagaaa ccaatggcat tgttgccggg ccccgtggcg
   127801 cggcgacacg attagggatg aagcgcacca cgctgctgtc acgaatgcag cgtctgggaa
   127861 tctcggttcg cgaggtgttg taatctgctt ttgcaggagt atgcatgaga aacaaactct
   127921 ctttcgactt gcagttgagc gccagaaaag cggcaatcgc tgaacggatt gccgcccata
   127981 aaattgcccg cagtaaagtg tcggtctttt taatggcgat gtccgctggc gtgtttatgg
   128041 cgatcggatt tactttttac ctttccgtta tcgccgatgc cccgtcttca taggcattaa
   128101 cccatctggt gggcggcctt tgctttacac ttggctttat tttgctggcg gtttgcggca
   128161 ccagcctgtt cacctcgtcg gtaatgacgg taatggcaaa aagtcggggc gttattagtt
   128221 ggcgaacttg gctgattaac gcacttctgg tggcctgcgg taatctggca ggtattgcct
   128281 gtttcagttt gttaatctgg ttttccgggc tggtgatgag tgaaaacgcg atgtggggag
   128341 tcgcgatttt acactgcgcc gagggcaaaa tgcatcatac atttactgaa tctgtcagcc
   128401 tcggcattat gtgcaatctg atggtttgcc tggcgctgtg gatgagttat tgcggacgtt
   128461 cgttatgcga caaaatcgtc gccatgattt tgtccatcac cctgtttgtc gccagtggct
   128521 ttgagcactg tatcgccaat ttgtttgtga ttccgttcgc cattgccatt cgccatttcg
   128581 cccctacctc cttctggcaa ctggcgcaca gtagcgcaga caattttccg gcactgacgg
   128641 tcagccattt tattaccgcc aatctgctcc cggtgatgct gggtaatatt atcggcggtg
   128701 cagtgctggt gagtatttgt tatcgggcta tttatttacg tcaggaatcc tgaaaaatca
   128761 gcccggcgaa acagttcgtc gggctgaaga ttattcttgc gcgatttgcc catcgggcca
   128821 ggcgtggatc acggctttga tcagcgtcgc caatggaatg gcgaagaata cgccccagaa
   128881 tccccacaaa ccaccgaaga tcaccaccga taaaataatc accagcggat gcaggttaac
   128941 cgcttcggag aacaacaccg gtactagcag gttgccgtcc agcgcctgaa taatcagata
   129001 caccgcgaag cagctccaga attcagtacc tgcgccaaac tggaatagcg ccacgccaac
   129061 caccggaatg gtcaccacaa atgcgccaat gtacggaata agaaccgaga agccgaccag
   129121 cactgccagc agcagcgaat agttcagccc gaagagcaag aaccccagcc aggtggcgat
   129181 ccccaccacg atcatctcca gcactttgcc gcggatatag ttggtgattt gttgattcat
   129241 ctccttccac acctgtcctg ccagcccacg gttacgcggc agcacccggc gaacggcgtt
   129301 cagcatctgc tctttgtctt tcagcaggaa gaagaccatc aatggcacca gcaccagata
   129361 gacggctatg gtcagcaaac cgaccagcga ggcgagggaa attttcacca ccgaatcgcc
   129421 catggtcagc atccgactgc gcatattttc ggccattgca tcaataatgc ccgcgtccat
   129481 taacaccgga tagcggcgcg gcaacgtggc ggcaaagtca gaaagcttat tgagcatccc
   129541 cggcatatcg cggattaagt agatgccctg ttgccaggcg ataggcagta ccacgaacgc
   129601 catcagtagc aatataccga caaaaaccac caatacaatc gacgtcgccc agcggcggga
   129661 gcagccaata gattgcaggc gcacggttgg ccattccagc aaataagcca gcacaatagc
   129721 caccagcaac ggagcaagca ggccactaaa gaaaaagata atgccaaatc cggcaactaa
   129781 aataaccagc aaggcaatcg cttccgggtc gctaaaacgg cggcgatacc attgcatcaa
   129841 catttcgagc ataaagacct tccctgaacc tcaagagcgg gattgcgatc cgcaattgta
   129901 tcgaaatgtc acaaaaaaga cttcgctttt tatgacggat tcaggaaact gaaaagtcat
   129961 ttgagtgggc taatcttcgc cgttacactc aaaggcggcg aggtgggaac gatatttcac
   130021 agtatcggtc aaatgactat cttcagaaat acaggataga ggttatgttc aggcagttga
   130081 aaaaaaacct ggttgcaacc ctcattgctg ctatgaccat tggtcaggta gccccggcgt
   130141 ttgccgacag cgcagacacc ttgccggata tgggaacctc cgcaggaagc acgctttcca
   130201 ttggtcagga aatgcagatg ggcgactatt atgtccgcca gctacgcggc agcgcgccgt
   130261 taattaatga cccgctgtta acgcaatata ttaattcgct ggggatgcgt ctggtttcgc
   130321 atgccaattc ggttaagaca ccgtttcatt tttttctgat caacaacgac gaaattaacg
   130381 cctttgcttt ctttggcggc aacgtggtgc tgcactctgc cctgttccgt tattccgata
   130441 acgaaagtca actggcttca gttatggcgc acgaaatctc ccacgtcacc caacgtcacc
   130501 tggcgcgagc gatggaagat caacaacgca gcgcgccgct gacctgggtc ggcgcgttag
   130561 gttctatttt actggcgatg gccagtccgc aggcggggat ggcggcgctg accggtacac
   130621 tggcgggaac gcgccagggg atgatcagtt tcacccagca aaatgaacag gaagcggacc
   130681 gcattggtat tcaggtgctg caacgctcgg gattcgatcc acaggcgatg ccgaccttcc
   130741 tggaaaaatt gctcgatcag gcgcgttact cctcgcgccc gccggaaatt ttattgactc
   130801 acccgttgcc ggaaagtcgt ctggcagatg cccgcaaccg tgctaatcag atgcgcccga
   130861 tggtggtgca gtcgtcggaa gatttctatc tggcgaaagc gcgcacactg gggatgtata
   130921 attccggacg taaccagctc accagtaatt tgctggatga atgggcgaaa ggaaacgttc
   130981 ttcagcaacg agcggcgcaa tatggtcgtg ctttacaggc gatgaaagcc aataaatacg
   131041 acgaggcgcg taaaacgctg caaccgttac tggcggcaga acctggcaac gcatggtatc
   131101 tcgatctggc taccgatatc gatcttgagc aaaacaaagc caatgaggca atcaatcgcc
   131161 tgaaaaatgc ccgcgattta cgcaccaatc cggtgttgca gctcaacctg gcgaacgctt
   131221 atctgcaagg cggtcaacca caagaagcgg ccaatattct gaatcgctac acctttaata
   131281 ataaagatga cagcaacggc tgggatttgc tggcacaggc ggaagccgcg ctaaataacc
   131341 gcgatcagga gctggctgcg cgagcagaag gttatgcgct cgccggacga ctcgatcagg
   131401 ccatttcgct gttgagtagc gccagttcgc aggtgaaatt aggcagcctg caacaagcgc
   131461 gttacgatgc gcgcatcgac cagttgcgcc agctgcagga acgctttaag ccttatacca
   131521 agatgtaatt cgcattaaag gaagaacaat gaccaaacag gtaaaaatct accataaccc
   131581 acgctgttca aagagccggg aaacgctgaa cctgctgaaa gaaaacggcg tggagccaga
   131641 agtggtgctc taccttgaga cacccgccga tgcggcaacg ctgcgcgatt tgctgaaaat
   131701 gctggggatg aatagcgccc gtgaattgat gcgccagaaa gaggatcttt ataaagaact
   131761 aaacctggct gacagttcgc ttagcgaaga ggcgctgatt caggcaatgg tggataatcc
   131821 gaagctgatg gagcgcccga ttatggtcgc gaatggcaaa gcgcggattg gtcgtccacc
   131881 ggagcaggta ctggaaatcg tgggttaaga tgtgctccat cgctagttga agcacattgc
   131941 cggatgcgac gcttgccacg tcttatccgg cctacgaata ctatggatta acttcgcgta
   132001 ggtcaggcgt tcgcgccgca tccgacaata aacaccttat ctacaacttc agaatttctt
   132061 tcacaaacgg aatggtcagc ttacgttgcg cggtaatcga cgcacgatcc aactgatcca
   132121 acgtcataaa tagcgtgcgc atttctctgt cgagccgctt cagcaagaaa cgccccacat
   132181 cttccggcag ttcaaaacca cgcaaacgcg cgcgtaactg tagcgcctgc aacttatctt
   132241 catcagaaag tggctgcaat ttgtagatct gcccccagtc gagacgcgac gcgagatccg
   132301 gtaatcccag attcaactgc cgcggtggac gatcgccggt gatcaacaac cgtgttttgc
   132361 ccgattccag aattcgattg tagagatcga aaatcgccat ctcccacaac tcatcgcctg
   132421 caatacactc aatgttgtcg atacagacca gcgacaaatg ctccataccg tcgagcactt
   132481 ccggaacaaa ccaggtgcgt ttatccagcg ggacatagcc caccgcatcg ccacgctgcg
   132541 acaattccgc gcaagccgcg tgcagcagat ggctgcgccc cgcgccttcg cgtgcccaga
   132601 gatagatgta accgctatgt tcctgacgca gcacgttttg cagcgcggcc agtaaagagg
   132661 agttatcccc cggccagaaa cttgcaaagg tttcgtcgtc aggaagataa agtggcaaag
   132721 agagctgtgc cggtgtgttc agagatacct caaccaggat ttcacaaaat cgcgagaagt
   132781 ttaccacaga atcccatgat gtttgaaccg ggcagcaaca ctgcccggtc ggtacattat
   132841 ttgtctgtta tatccgcgtc ttctgcgtcc agcaccactt cttcagggcg cagcacactg
   132901 atcagtttga aaatcaaact taagccgata ccgacgatgg tcgccagcgc catacctttt
   132961 aactccgccg cgccgatgtt taccttcgcg ccactgacgc cgatgatcaa aatcacggaa
   133021 gtcaggatca ggttctgtgc tttgttgtaa tccactttcg attcgatcaa aacacgaata
   133081 ccggaagcac caatgacacc ataaagcagc agcgaaacgc cgcccatcac cggcaatggg
   133141 atcatctgga tagcggcagc cagtttaccg acgcaggaaa gcaggatagc gaaaatcgcc
   133201 gccccgccga taacccaggt actgtaaaca cgggtgatcg ccatcacgcc aatgttttct
   133261 ccgtaagtgg tatttggcgt agagccaaag aagccggaga tcacggtcga caggccgtta
   133321 gcaaacatcg aacggtgcag acctggatcg cgcagcagat cttttttgac gatattagcc
   133381 gttactacca ggtgccctac gtgttcggca ataaccacta acgccgctgg cagaatagtc
   133441 agaatggcaa accactcgaa gcgcggcgta tagagggttg gcagtgcaaa ccagtgagca
   133501 ttaataatcg gcgtggtatc gacaattccc attgcgaaag agagcgcgta ccccaccagc
   133561 acgccaatta aaatcgggat aattgccagg aaaccacgaa acagcacgga acctaaaacc
   133621 gtgaccgcca gggtggtaat agagatgatg atggttttgg agtttggcgt ttgcccttca
   133681 gccgggagta aacccgccat accggcagct acgcccgcca gctccagacc gatgacggca
   133741 acgattgcgc ccattgccgc aggtggaaac agcacgtcca accagccggt ccccgctttc
   133801 ttcacgataa aagaaaccag gcagaacagc acgccgcaca taataaagcc gcccagcgcg
   133861 acttcatacc ctaacggcaa cagtaacaat accggtgaaa taaaggcaaa gctggaacca
   133921 agataagccg gaattttccc tttacagatg aagagataca gcagcgttcc aataccgtta
   133981 aataacagta cagtcgccgg gttaatatga aataagacgg gcaccaggac ggttgcacca
   134041 aacatggcga acaaatgttg caaactaagc gggattgtct gtaaaagtgg cggtctttca
   134101 ctcaccccga tagcacggcg cgtcatagta ttatcctctg tattatgtgt tataggcgct
   134161 ttactcaaaa aaaagccgac tcttaaagtc ggctttaatt atttttattc tttatttcgt
   134221 accaaagatt ttgtcaccgg catcgccgag gcccggaata atgtatccgt gctcgttcag
   134281 tccctggtca atcgatgcag tatacagttc gacgtccggg tgcgcttttt ccagcgcagc
   134341 gataccttct ggcgcagcga ccagcaccag aactttgatg ctgctgcagc ccgctttttt
   134401 cagcaggtcg atggtcgcga taacggaacc accggttgcc agcattgggt caacgatcag
   134461 cgccatacgc tcatcgatgt tagaaaccag tttctggaag tacggtaccg gctccagcgt
   134521 ttcttcatta cggtacatac cgacaacgct gatgcgcgcg ctcggaacgt tttccagcac
   134581 accgtccatc ataccaagac ccgcacgcag aattggcaca acggtaattt tcttaccttt
   134641 gatctggtcg atttctaccg ggccgttcca gccttcgata gttacttttt ccgtttcgag
   134701 gtcggcggtc gcttcgtaag tcagcaggct acccacttcg gaagcgagtt cgcgaaagcg
   134761 cttggtgctg atatcttgct cacgcatcag tcccagcttg tgtttgacga gtgggtgttt
   134821 gacttccacg atcttcatac tctttctcct ttgaggggca gccacaaaaa aaatcgaagg
   134881 attatacctc ctttcttcaa ggcggcaata ttattttcgt tgactttagt caaaatgata
   134941 acagtttgag ataaagttat tttatattca aatggttatg aaagaagatt attccatccg
   135001 aaaactaacc tttgccctgg cacaagtctt ctttcgccgc gcgcccgggg aaaagacgtg
   135061 caaaaaggtt gtgtaaaaca gtctcgcaaa cgtttgcttt ccctgttaga attgcgccga
   135121 attttatttt tctaccgcaa gtaacgcgtg gggacccaag cagtgaccga taaaacctct
   135181 cttagctaca aagatgccgg tgttgatatt gacgcgggta atgctctggt tggaagaatc
   135241 aaaggcgtag tgaagaaaac gcgtcgtccg gaagtgatgg gcggtctggg cggcttcggt
   135301 gcgctgtgtg cattgccgca aaaatatcgt gaacccgtgc tggtttccgg cactgacggc
   135361 gtaggtacca agctgcgtct ggcaatggac ttaaaacgtc acgacaccat tggtattgat
   135421 ctggtcgcca tgtgcgttaa tgacctggtg gtgcaaggtg cggaaccgct gtttttcctc
   135481 gactattacg caaccggaaa actggatgtt gataccgctt cagcagtgat cagcggcatc
   135541 gcggaaggtt gtctgcaatc gggctgttct ctggtgggtg gcgaaacggc agaaatgccg
   135601 gggatgtatc acggtgaaga ttacgatgtc gcgggtttct gcgtgggcgt ggtagaaaaa
   135661 tcagaaatca tcgacggctc taaagtcagc gacggcgatg tgctgattgc actcggttcc
   135721 agcggtccgc actcgaacgg ctattcgctg gtgcgcaaaa ttcttgaagt cagcggttgt
   135781 gatccgcaaa ccaccgaact tgatggtaag ccattagccg atcatctgct ggcaccgacc
   135841 cgcatttacg tgaagtcagt gctggagttg attgaaaagg tcgatgtgca tgccattgcg
   135901 cacctgaccg gcggcggctt ctgggaaaac attccgcgcg tattgccaga taatacccag
   135961 gcagtgattg atgaatcttc ctggcagtgg ccggaagtgt tcaactggct gcaaacggca
   136021 ggtaacgttg agcgccatga aatgtatcgc accttcaact gcggcgtcgg gatgattatc
   136081 gccctgcctg ctccggaagt ggacaaagcc ctcgccctgc taaatgccaa cggtgaaaac
   136141 gcgtggaaaa tcggtatcat caaagcctct gattccgaac aacgcgtggt tatcgaataa
   136201 tgaatattgt ggtgcttatt tccggcaacg gaagtaattt acaggcaatt attgacgcct
   136261 gtaaaaccaa caaaattaaa ggcaccgtac gggcagtttt cagcaataag gccgacgcgt
   136321 tcggccttga acgcgcccgc caggcgggta ttgcaacgca tacgctcatc gccagcgcgt
   136381 ttgacagtcg tgaagcctat gaccgggagt tgattcatga aatcgacatg tacgcacccg
   136441 atgtggtcgt gctggctggt tttatgcgca ttctcagccc ggcgtttgtc tcccactatg
   136501 ccgggcgttt gctgaacatt cacccttctc tgctgccgaa atatcccgga ttacacaccc
   136561 atcgtcaggc gctggaaaat ggcgatgaag agcacggtac atcggtgcat ttcgtcaccg
   136621 atgaactgga cggtggcccg gttattttac aggcgaaagt cccggtattt gctggtgata
   136681 cggaagatga catcaccgcc cgcgtgcaaa cccaggaaca cgctatttat ccactggtga
   136741 ttagctggtt tgccgatggt cgtctgaaaa tgcacgaaaa cgccgcgtgg ctggatggtc
   136801 aacgtctgcc gccgcagagc tacgctgccg acgagtaatg cccccgtagt taaagcgcca
   136861 gctctgccgc tggcgttttt caattcacct gtaaatcgca agctccagca gtttttttcc
   136921 cccttttctg gcatagttgg acatctgcca atattgctcg ccataatatc caggcagtgt
   136981 cccgtgaata aaacggagta aaagtggtaa tgggtcagga aaagctatac atcgaaaaag
   137041 agctcagttg gttatcgttc aatgaacgcg tgcttcagga agcggcggac aaatctaacc
   137101 cgctgattga aaggatgcgt ttcctgggga tctattccaa taaccttgat gagttctata
   137161 aagtccgctt cgctgaactg aagcgacgca tcattattag cgaagaacaa ggctccaacg
   137221 ctcattcccg ccatttactg ggcaaaattc agtcccgggt gctgaaagcc gatcaggaat
   137281 tcgacggcct ctacaacgag ctattgctgg agatggcgcg caaccagatc ttcctgatta
   137341 atgaacgcca gctctccgtc aatcaacaaa actggctgcg tcattatttt aagcagtatc
   137401 tgcgtcagca cattacgccg attttaatca atcctgacac tgacttagtg cagttcctga
   137461 aagatgatta cacctatctg gcggtggaaa ttatccgtag cgatagcatc cgttacgcgc
   137521 tgctggagat cccatcagat aaagtgccgc gctttgtgaa tttaccgcca gaagcgccgc
   137581 gtcgacgcaa gccgatgatt cttctggata acattctgcg ttactgcctt gatgatattt
   137641 tcaaaggctt ctttgattat gacgcgctga atgcctattc aatgaagatg acccgcgatg
   137701 ccgaatacga tttagtgcat gagatggaag ccagcctgat ggagttgatg tcttccagtc
   137761 tcaagcagcg tttaactgct gagccggtgc gttttgttta tcagcgcgat atgcccaatg
   137821 cgctggttga agtgttacgc gaaaaactga ctatttcccg ctacgactcc atcgtccccg
   137881 gcggtcgtta tcataatttt aaagacttta ttaattttcc caatgtcggc aaagccaatc
   137941 tggtgaacaa accactgccg cgtttacgcc atatttggtt tgataaagcc cagttccgca
   138001 atggttttga tgccattcgc gaacgcgatg tgttgctcta ttatccttat cacacctttg
   138061 ggcatgtgct ggaactgctg cgtcaggctt cgttcgaccc gagcgtactg gcgattaaaa
   138121 ttaacattta ccgcgtggcg aaagattcac gcatcatcga ctcgatgatc cacgccgcac
   138181 ataacggtaa gaaagtcact gtggtggttg agttacaggc gcgtttcgac gaagaagcca
   138241 acattcactg ggcgaagcgc ctgaccgaag caggcgtgca cgttatcttc tctgcgccgg
   138301 ggctgaaaat tcacgccaaa ctgttcctga tttcacgtaa agaaaacggt gaagtggtcc
   138361 gttacgcaca catcgggacc gggaacttta acgaaaaaac cgcgcgtctt tatactgact
   138421 attcgttgct gaccgcagat gcgcgtatca ccaacgaagt acggcgggta tttaacttta
   138481 ttgaaaaccc ataccgcccg gtgacatttg attatttaat ggtgtcaccg caaaactctc
   138541 gccgtctgtt atatgaaatg gtagaccgcg aaatcgccaa cgcgcagcaa gggctgccca
   138601 gtggtatcac cctgaagcta aataaccttg tcgataaagg cctggttgat cgtctgtatg
   138661 cggcctccag ctccggcgta ccggttaatc tgctggttcg cggaatgtgt tcgctgatcc
   138721 ccaatctgga aggcattagc gacaacattc gtgccatcag tattgttgac cgttaccttg
   138781 aacatgaccg ggtttatatt tttgaaaatg gcggcgataa aaaggtctac ctttcttccg
   138841 ccgactggat gacgcgcaat attgattatc gtattgaagt ggcgacaccg ctgctcgatc
   138901 cgcgcctgaa gcagcgggtg ctggacatca tcgacatatt gttcagcgat acggtcaaag
   138961 cacgttatat cgataaagaa ctcagtaatc gctacgttcc ccgcggcaat cgccgcaaag
   139021 tacgggcgca gttggcgatt tacgactaca tcaaatcact cgaacaacct gaataaccct
   139081 atgccaatac acgataaatc ccctcgtccg caggagtttg ctgcggtcga tcttggttca
   139141 aacagttttc acatggtcat agcccgtgtg gtagatggtg ccatgcagat tattggccgc
   139201 ctgaaacagc gggtgcatct ggcggacggc ctggggccag ataatatgtt gagtgaagag
   139261 gcaatgacgc gcggtttgaa ctgtctgtcg ctgtttgccg aacggctaca agggttttct
   139321 cctgccagcg tctgtatagt cggcacccat acgctgcgtc aggcgctgaa cgccactgac
   139381 tttctgaaac gcgcggaaaa ggtcattccc tacccgattg aaattatttc cggtaatgaa
   139441 gaagcccgtc tgatttttat gggcgtggaa catacccaac cggaaaaagg tcgcaaactg
   139501 gttattgata ttggcggcgg atctacggaa ctggtgattg gtgaaaattt cgaacctatt
   139561 ctcgttgaaa gccgccggat gggttgtgtc agctttgccc agctttattt tcctggcggg
   139621 gtcatcaata aagagaattt tcagcgcgct cgcatggcgg cagcacaaaa actggaaact
   139681 ttaacctggc aattccgtat tcagggctgg aacgttgcaa tgggcgcttc cggtaccata
   139741 aaagccgccc atgaagtgtt aatggaaatg ggcgagaaag acgggataat taccccggaa
   139801 cgtctggaaa aactggtaaa agaaatttta cgtcaccgta atttcgcatc gctgagttta
   139861 ccgggtcttt ccgaagagcg gaaaacagtc ttcgttcctg gactggcgat tttatgtggt
   139921 gtgtttgatg ctttagccat ccgtgaactg cgcctttctg acggggcgtt acgcgaaggc
   139981 gtactgtatg aaatggaagg acgtttccgt catcaggatg tgcgtagtcg caccgccagc
   140041 agcctcgcca accagtatca catagacagc gaacaggccc gacgagtgct ggataccact
   140101 atgcaaatgt acgaacagtg gcgggaacag caaccgaagc tggcgcatcc gcaactggag
   140161 gcgctactgc gatgggccgc catgctgcat gaggtcgggt tgaatatcaa ccacagcggt
   140221 ttgcatcgcc actccgctta tattctgcaa aacagtgact tgccgggttt taatcaggaa
   140281 cagcagctga tgatggcgac actgctgcgc tatcaccgta aagcgattaa gctcgacgat
   140341 ctgccgcgct ttaccttgtt taagaagaaa cagttcctgc cactgataca gctattgcgc
   140401 cttggcgtat tactaaacaa tcaacgtcag gcaaccacca caccgtcaac attgacactg
   140461 attactgatg acagtcactg gacactgcgt ttcccgcatg actggtttag tcagaatgcg
   140521 ctggtactgc ttgatctgga aaaggagcaa gaatactggg aaggcgtggc tggctggcgg
   140581 ttgaaaattg aagaagaaag taccccagaa atcgccgctt aatgctttgc gggccgacga
   140641 gaaatgtcgg cccgcattat tcaggcactt tcgcgaatgg gttcgatttc attcagcgta
   140701 tcaattaacg gttgcggctt accaataaga taaccctgca tataatcgat ccccaaagag
   140761 agcaccgcct cgcggatctc ttcgttttca acgtactctg ccactacccg cattttcttc
   140821 attcgcgcca gatggcaaat cgacgccaca atctgataat ccagactatt tgacacaata
   140881 ttgcggataa agctgccgtc aattttaagc agatcggcat tcacattttt aagccgtgca
   140941 tagctggcgt agccggtgcc gaaatcatca atcgcaatct ggcagcctaa ttcctgaaga
   141001 tgctgcaagg tgatttgcgc ctgcttaaca ttggtcagag cattactttc ggtgacttca
   141061 aaaataagtt gccacgcttc aatctgatat ttagccagca actgactgac ttcaacagga
   141121 aaacgagcct gacataccga ggttggagac agattaatag caaaacggtg agcgggcatc
   141181 ttcgctctgt tttcagccat aaattgcagc gtatgctcaa tgacccacat gtcgatactc
   141241 gacgataaac caaattcgtg cgcgaccggc aagaagctat cggggccgat cagttcatca
   141301 ttctcacctt tcatgcgcag aagaatttca tggtaaacat caccacgcat accggtaatc
   141361 ggctgggcca tcaggaaaaa atggttgtgc tccagcgcct gctgtagtcg attcatcatc
   141421 gcgactttat ctttcaattc gcgttgcaaa tacattgccc cgcaacgctg catattttcc
   141481 ggggcgttgg tcacgatgga aagttcggcg accgtattta gctctcccag cagcaggtag
   141541 atatgattca ctggcgagcg cacatagcag taactgacgc caatctgcgg ttgcatcggc
   141601 atcccatccc aaaagaaacg aaattgcttg agatgactat ccagtgcggt aatgcgctcc
   141661 tggtgcgatt ctgtattcag tcgcagcgcg agatcgttac ccgaaagctg ataaacatct
   141721 tcacccggtt ccagcaaggg tgacagccag tgagaaagt
//
LOCUS       DAJTYF010000002       111736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTYF010000002 DAJTYF010000000
VERSION     DAJTYF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 111736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 111736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..111736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.002"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            608..2893
                     /gene="nrdA"
                     /locus_tag="OO590_000134"
     CDS             608..2893
                     /gene="nrdA"
                     /locus_tag="OO590_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6635642.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3127..4257
                     /gene="nrdB"
                     /locus_tag="OO590_000135"
     CDS             3127..4257
                     /gene="nrdB"
                     /locus_tag="OO590_000135"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6635643.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4257..4511
                     /gene="yfaE"
                     /locus_tag="OO590_000136"
     CDS             4257..4511
                     /gene="yfaE"
                     /locus_tag="OO590_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6635644.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4565..5215)
                     /gene="inaA"
                     /locus_tag="OO590_000137"
     CDS             complement(4565..5215)
                     /gene="inaA"
                     /locus_tag="OO590_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6635645.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5379..5621
                     /locus_tag="OO590_000138"
                     /pseudo
     CDS             5379..5621
                     /locus_tag="OO590_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5663..6739)
                     /gene="glpQ"
                     /locus_tag="OO590_000139"
     CDS             complement(5663..6739)
                     /gene="glpQ"
                     /locus_tag="OO590_000139"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6635646.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6744..8102)
                     /gene="glpT"
                     /locus_tag="OO590_000140"
     CDS             complement(6744..8102)
                     /gene="glpT"
                     /locus_tag="OO590_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6635647.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8375..10003
                     /gene="glpA"
                     /locus_tag="OO590_000141"
     CDS             8375..10003
                     /gene="glpA"
                     /locus_tag="OO590_000141"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6635648.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9993..11252
                     /gene="glpB"
                     /locus_tag="OO590_000142"
     CDS             9993..11252
                     /gene="glpB"
                     /locus_tag="OO590_000142"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6635649.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11249..12439
                     /gene="glpC"
                     /locus_tag="OO590_000143"
     CDS             11249..12439
                     /gene="glpC"
                     /locus_tag="OO590_000143"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6635650.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12632..13531
                     /locus_tag="OO590_000144"
     CDS             12632..13531
                     /locus_tag="OO590_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6635651.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13535..13771
                     /locus_tag="OO590_000145"
                     /pseudo
     CDS             13535..13771
                     /locus_tag="OO590_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13812..14282)
                     /locus_tag="OO590_000146"
     CDS             complement(13812..14282)
                     /locus_tag="OO590_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6635652.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14337..15539)
                     /locus_tag="OO590_000147"
     CDS             complement(14337..15539)
                     /locus_tag="OO590_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6635653.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSSRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15626..16168)
                     /locus_tag="OO590_000148"
     CDS             complement(15626..16168)
                     /locus_tag="OO590_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635654.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16447..16872
                     /gene="nudI"
                     /locus_tag="OO590_000149"
     CDS             16447..16872
                     /gene="nudI"
                     /locus_tag="OO590_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6635655.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16911..17513)
                     /locus_tag="OO590_000150"
     CDS             complement(16911..17513)
                     /locus_tag="OO590_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6635656.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNMVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17821..18960
                     /gene="arnB"
                     /locus_tag="OO590_000151"
     CDS             17821..18960
                     /gene="arnB"
                     /locus_tag="OO590_000151"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6635657.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18964..19932
                     /gene="arnC"
                     /locus_tag="OO590_000152"
     CDS             18964..19932
                     /gene="arnC"
                     /locus_tag="OO590_000152"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6635658.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19932..21914
                     /gene="arnA"
                     /locus_tag="OO590_000153"
     CDS             19932..21914
                     /gene="arnA"
                     /locus_tag="OO590_000153"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR6635659.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDFVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21911..22801
                     /locus_tag="OO590_000154"
     CDS             21911..22801
                     /locus_tag="OO590_000154"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6635660.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22801..24453
                     /gene="arnT"
                     /locus_tag="OO590_000155"
     CDS             22801..24453
                     /gene="arnT"
                     /locus_tag="OO590_000155"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6635661.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDLWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24450..24785
                     /gene="arnE"
                     /locus_tag="OO590_000156"
     CDS             24450..24785
                     /gene="arnE"
                     /locus_tag="OO590_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6635662.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24785..25171
                     /gene="arnF"
                     /locus_tag="OO590_000157"
     CDS             24785..25171
                     /gene="arnF"
                     /locus_tag="OO590_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6635663.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25165..25437)
                     /gene="pmrD"
                     /locus_tag="OO590_000158"
     CDS             complement(25165..25437)
                     /gene="pmrD"
                     /locus_tag="OO590_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6635664.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25542..26897)
                     /gene="menE"
                     /locus_tag="OO590_000159"
     CDS             complement(25542..26897)
                     /gene="menE"
                     /locus_tag="OO590_000159"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6635665.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADVLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26894..27856)
                     /gene="menC"
                     /locus_tag="OO590_000160"
     CDS             complement(26894..27856)
                     /gene="menC"
                     /locus_tag="OO590_000160"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6635666.1"
                     /translation="MRSAQVYRWQIPMDAGVLLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27856..28713)
                     /gene="menB"
                     /locus_tag="OO590_000161"
     CDS             complement(27856..28713)
                     /gene="menB"
                     /locus_tag="OO590_000161"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6635667.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28728..29486)
                     /gene="menH"
                     /locus_tag="OO590_000162"
     CDS             complement(28728..29486)
                     /gene="menH"
                     /locus_tag="OO590_000162"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6635668.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29483..31153)
                     /gene="menD"
                     /locus_tag="OO590_000163"
     CDS             complement(29483..31153)
                     /gene="menD"
                     /locus_tag="OO590_000163"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6635669.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31242..32537)
                     /gene="menF"
                     /locus_tag="OO590_000164"
     CDS             complement(31242..32537)
                     /gene="menF"
                     /locus_tag="OO590_000164"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6635670.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32616..32921)
                     /gene="elaB"
                     /locus_tag="OO590_000165"
     CDS             complement(32616..32921)
                     /gene="elaB"
                     /locus_tag="OO590_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6635671.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32976..33437)
                     /locus_tag="OO590_000166"
     CDS             complement(32976..33437)
                     /locus_tag="OO590_000166"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6635672.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33502..34419
                     /gene="rbn"
                     /locus_tag="OO590_000167"
     CDS             33502..34419
                     /gene="rbn"
                     /locus_tag="OO590_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6635673.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TAQRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34607..35827
                     /gene="elaD"
                     /locus_tag="OO590_000168"
     CDS             34607..35827
                     /gene="elaD"
                     /locus_tag="OO590_000168"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR6635674.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGNEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSELMGKMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
     gene            36247..36862
                     /locus_tag="OO590_000169"
                     /pseudo
     CDS             36247..36862
                     /locus_tag="OO590_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            36965..37468
                     /locus_tag="OO590_000170"
     CDS             36965..37468
                     /locus_tag="OO590_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCR6635675.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(37535..38992)
                     /gene="nuoN"
                     /locus_tag="OO590_000171"
     CDS             complement(37535..38992)
                     /gene="nuoN"
                     /locus_tag="OO590_000171"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6635676.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFDYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(38999..40528)
                     /gene="nuoM"
                     /locus_tag="OO590_000172"
     CDS             complement(38999..40528)
                     /gene="nuoM"
                     /locus_tag="OO590_000172"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6635677.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(40692..42533)
                     /gene="nuoL"
                     /locus_tag="OO590_000173"
     CDS             complement(40692..42533)
                     /gene="nuoL"
                     /locus_tag="OO590_000173"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6635678.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAAQV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(42530..42832)
                     /gene="nuoK"
                     /locus_tag="OO590_000174"
     CDS             complement(42530..42832)
                     /gene="nuoK"
                     /locus_tag="OO590_000174"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6635679.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(42829..43383)
                     /gene="nuoJ"
                     /locus_tag="OO590_000175"
     CDS             complement(42829..43383)
                     /gene="nuoJ"
                     /locus_tag="OO590_000175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6635680.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(43395..43937)
                     /gene="nuoI"
                     /locus_tag="OO590_000176"
     CDS             complement(43395..43937)
                     /gene="nuoI"
                     /locus_tag="OO590_000176"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6635681.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(43952..44929)
                     /gene="nuoH"
                     /locus_tag="OO590_000177"
     CDS             complement(43952..44929)
                     /gene="nuoH"
                     /locus_tag="OO590_000177"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6635682.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(44926..47652)
                     /gene="nuoG"
                     /locus_tag="OO590_000178"
     CDS             complement(44926..47652)
                     /gene="nuoG"
                     /locus_tag="OO590_000178"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6635683.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(47705..49042)
                     /gene="nuoF"
                     /locus_tag="OO590_000179"
     CDS             complement(47705..49042)
                     /gene="nuoF"
                     /locus_tag="OO590_000179"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6635684.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(49039..49539)
                     /gene="nuoE"
                     /locus_tag="OO590_000180"
     CDS             complement(49039..49539)
                     /gene="nuoE"
                     /locus_tag="OO590_000180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6635685.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(49542..51344)
                     /gene="nuoC"
                     /locus_tag="OO590_000181"
     CDS             complement(49542..51344)
                     /gene="nuoC"
                     /locus_tag="OO590_000181"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6635686.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(51438..52100)
                     /gene="nuoB"
                     /locus_tag="OO590_000182"
     CDS             complement(51438..52100)
                     /gene="nuoB"
                     /locus_tag="OO590_000182"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6635687.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(52116..52559)
                     /gene="nuoA"
                     /locus_tag="OO590_000183"
     CDS             complement(52116..52559)
                     /gene="nuoA"
                     /locus_tag="OO590_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6635688.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(53190..54128)
                     /gene="lrhA"
                     /locus_tag="OO590_000184"
     CDS             complement(53190..54128)
                     /gene="lrhA"
                     /locus_tag="OO590_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6635689.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            55092..56309
                     /gene="alaA"
                     /locus_tag="OO590_000185"
     CDS             55092..56309
                     /gene="alaA"
                     /locus_tag="OO590_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6635690.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            56393..56992
                     /gene="yfbR"
                     /locus_tag="OO590_000186"
     CDS             56393..56992
                     /gene="yfbR"
                     /locus_tag="OO590_000186"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6635691.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(57051..58883)
                     /locus_tag="OO590_000187"
     CDS             complement(57051..58883)
                     /locus_tag="OO590_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6635692.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(58970..59620)
                     /gene="hxpA"
                     /locus_tag="OO590_000188"
     CDS             complement(58970..59620)
                     /gene="hxpA"
                     /locus_tag="OO590_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6635693.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALSGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(59631..60125)
                     /locus_tag="OO590_000189"
     CDS             complement(59631..60125)
                     /locus_tag="OO590_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6635694.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(60208..60663)
                     /locus_tag="OO590_000190"
     CDS             complement(60208..60663)
                     /locus_tag="OO590_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6635695.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            61001..62203
                     /gene="ackA"
                     /locus_tag="OO590_000191"
     CDS             61001..62203
                     /gene="ackA"
                     /locus_tag="OO590_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6635696.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            62278..64422
                     /gene="pta"
                     /locus_tag="OO590_000192"
     CDS             62278..64422
                     /gene="pta"
                     /locus_tag="OO590_000192"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6635697.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            64612..66132
                     /gene="yfcC"
                     /locus_tag="OO590_000193"
     CDS             64612..66132
                     /gene="yfcC"
                     /locus_tag="OO590_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6635698.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(66165..66707)
                     /gene="yfcD"
                     /locus_tag="OO590_000194"
     CDS             complement(66165..66707)
                     /gene="yfcD"
                     /locus_tag="OO590_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6635699.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(66765..67316)
                     /gene="yfcE"
                     /locus_tag="OO590_000195"
     CDS             complement(66765..67316)
                     /gene="yfcE"
                     /locus_tag="OO590_000195"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6635700.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(67372..68016)
                     /gene="yfcF"
                     /locus_tag="OO590_000196"
     CDS             complement(67372..68016)
                     /gene="yfcF"
                     /locus_tag="OO590_000196"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6635701.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            68152..68799
                     /gene="yfcG"
                     /locus_tag="OO590_000197"
     CDS             68152..68799
                     /gene="yfcG"
                     /locus_tag="OO590_000197"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6635702.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            68856..69218
                     /gene="folX"
                     /locus_tag="OO590_000198"
     CDS             68856..69218
                     /gene="folX"
                     /locus_tag="OO590_000198"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6635703.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            69239..70132
                     /locus_tag="OO590_000199"
     CDS             69239..70132
                     /locus_tag="OO590_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6635704.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(70180..71070)
                     /gene="rpnB"
                     /locus_tag="OO590_000200"
     CDS             complement(70180..71070)
                     /gene="rpnB"
                     /locus_tag="OO590_000200"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6635705.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIA
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(71267..72040)
                     /gene="hisP"
                     /locus_tag="OO590_000201"
     CDS             complement(71267..72040)
                     /gene="hisP"
                     /locus_tag="OO590_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6635706.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEACERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(72048..72764)
                     /locus_tag="OO590_000202"
     CDS             complement(72048..72764)
                     /locus_tag="OO590_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6635707.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(72761..73447)
                     /gene="hisQ"
                     /locus_tag="OO590_000203"
     CDS             complement(72761..73447)
                     /gene="hisQ"
                     /locus_tag="OO590_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6635708.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(73537..74319)
                     /gene="hisJ"
                     /locus_tag="OO590_000204"
     CDS             complement(73537..74319)
                     /gene="hisJ"
                     /locus_tag="OO590_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6635709.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(74540..75322)
                     /gene="argT"
                     /locus_tag="OO590_000205"
     CDS             complement(74540..75322)
                     /gene="argT"
                     /locus_tag="OO590_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6635710.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(75588..76157)
                     /gene="ubiX"
                     /locus_tag="OO590_000206"
     CDS             complement(75588..76157)
                     /gene="ubiX"
                     /locus_tag="OO590_000206"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6635711.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(76252..77769)
                     /gene="purF"
                     /locus_tag="OO590_000207"
     CDS             complement(76252..77769)
                     /gene="purF"
                     /locus_tag="OO590_000207"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6635712.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(77806..78294)
                     /gene="cvpA"
                     /locus_tag="OO590_000208"
     CDS             complement(77806..78294)
                     /gene="cvpA"
                     /locus_tag="OO590_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6635713.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(78553..79215)
                     /gene="dedD"
                     /locus_tag="OO590_000209"
     CDS             complement(78553..79215)
                     /gene="dedD"
                     /locus_tag="OO590_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6635714.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(79205..80473)
                     /gene="folC"
                     /locus_tag="OO590_000210"
     CDS             complement(79205..80473)
                     /gene="folC"
                     /locus_tag="OO590_000210"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6635715.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(80543..81457)
                     /gene="accD"
                     /locus_tag="OO590_000211"
     CDS             complement(80543..81457)
                     /gene="accD"
                     /locus_tag="OO590_000211"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6635716.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(81613..82272)
                     /locus_tag="OO590_000212"
     CDS             complement(81613..82272)
                     /locus_tag="OO590_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6635717.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(82355..83167)
                     /gene="truA"
                     /locus_tag="OO590_000213"
     CDS             complement(82355..83167)
                     /gene="truA"
                     /locus_tag="OO590_000213"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6635718.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(83167..84180)
                     /locus_tag="OO590_000214"
     CDS             complement(83167..84180)
                     /locus_tag="OO590_000214"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6635719.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(84246..85382)
                     /gene="pdxB"
                     /locus_tag="OO590_000215"
     CDS             complement(84246..85382)
                     /gene="pdxB"
                     /locus_tag="OO590_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6635720.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            85481..86476
                     /gene="flk"
                     /locus_tag="OO590_000216"
     CDS             85481..86476
                     /gene="flk"
                     /locus_tag="OO590_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6635721.1"
                     /translation="MIQPISGPPLGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(86473..87651)
                     /locus_tag="OO590_000217"
     CDS             complement(86473..87651)
                     /locus_tag="OO590_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6635722.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLCFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(87926..89146)
                     /gene="fabB"
                     /locus_tag="OO590_000218"
     CDS             complement(87926..89146)
                     /gene="fabB"
                     /locus_tag="OO590_000218"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6635723.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            89305..91311
                     /gene="mnmC"
                     /locus_tag="OO590_000219"
     CDS             89305..91311
                     /gene="mnmC"
                     /locus_tag="OO590_000219"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6635724.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(91432..91710)
                     /locus_tag="OO590_000220"
     CDS             complement(91432..91710)
                     /locus_tag="OO590_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6635725.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(91744..92292)
                     /gene="epmC"
                     /locus_tag="OO590_000221"
     CDS             complement(91744..92292)
                     /gene="epmC"
                     /locus_tag="OO590_000221"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6635726.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(92292..93101)
                     /locus_tag="OO590_000222"
     CDS             complement(92292..93101)
                     /locus_tag="OO590_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6635727.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(93101..93925)
                     /gene="mepA"
                     /locus_tag="OO590_000223"
     CDS             complement(93101..93925)
                     /gene="mepA"
                     /locus_tag="OO590_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6635728.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(93929..95014)
                     /gene="aroC"
                     /locus_tag="OO590_000224"
     CDS             complement(93929..95014)
                     /gene="aroC"
                     /locus_tag="OO590_000224"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6635729.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(95049..95981)
                     /gene="prmB"
                     /locus_tag="OO590_000225"
     CDS             complement(95049..95981)
                     /gene="prmB"
                     /locus_tag="OO590_000225"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6635730.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            96147..96698
                     /gene="smrB"
                     /locus_tag="OO590_000226"
     CDS             96147..96698
                     /gene="smrB"
                     /locus_tag="OO590_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6635731.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(96802..97692)
                     /locus_tag="OO590_000227"
     CDS             complement(96802..97692)
                     /locus_tag="OO590_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6635732.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(97757..98203)
                     /locus_tag="OO590_000228"
     CDS             complement(97757..98203)
                     /locus_tag="OO590_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6635733.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNT"
     gene            complement(98200..98670)
                     /locus_tag="OO590_000229"
     CDS             complement(98200..98670)
                     /locus_tag="OO590_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6635734.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(98667..99215)
                     /locus_tag="OO590_000230"
     CDS             complement(98667..99215)
                     /locus_tag="OO590_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6635735.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(99220..99942)
                     /locus_tag="OO590_000231"
     CDS             complement(99220..99942)
                     /locus_tag="OO590_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6635736.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPYQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSC"
     gene            complement(99962..100684)
                     /locus_tag="OO590_000232"
     CDS             complement(99962..100684)
                     /locus_tag="OO590_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6635737.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATFTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(100744..102603)
                     /locus_tag="OO590_000233"
     CDS             complement(100744..102603)
                     /locus_tag="OO590_000233"
                     /inference="COORDINATES: protein motif:HMM:NF011812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCR6635738.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGRTAVRSVISAVSMTRLTIS"
     gene            complement(102685..103248)
                     /locus_tag="OO590_000234"
     CDS             complement(102685..103248)
                     /locus_tag="OO590_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6635739.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(103932..104417)
                     /gene="sixA"
                     /locus_tag="OO590_000235"
     CDS             complement(103932..104417)
                     /gene="sixA"
                     /locus_tag="OO590_000235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6635740.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(104620..106764)
                     /gene="fadJ"
                     /locus_tag="OO590_000236"
     CDS             complement(104620..106764)
                     /gene="fadJ"
                     /locus_tag="OO590_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6635741.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(106764..108074)
                     /gene="fadI"
                     /locus_tag="OO590_000237"
     CDS             complement(106764..108074)
                     /gene="fadI"
                     /locus_tag="OO590_000237"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6635742.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGRFGLVTACAAGGLGAAMVLEAE"
     gene            complement(108254..108538)
                     /locus_tag="OO590_000238"
     CDS             complement(108254..108538)
                     /locus_tag="OO590_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6635743.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            108910..110250
                     /gene="fadL"
                     /locus_tag="OO590_000239"
     CDS             108910..110250
                     /gene="fadL"
                     /locus_tag="OO590_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6635744.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            110616..111674
                     /locus_tag="OO590_000240"
     CDS             110616..111674
                     /locus_tag="OO590_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635745.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNCQAK
                     YQNEINQLSALGVNFDDI"
BASE COUNT        28002 a        29874 c        27469 g        26391 t
ORIGIN      
        1 gatgcaatgg ctattttgtt attatttgat gaataatatc agtgcgtcat aattcaagtt
       61 aataacctta agggatacca gttatattta aactaaatta aagtaatgaa taattttctt
      121 ataatataag gtaaattaac aaaatggctt cgcatttaac aataaccgaa tagaaaacaa
      181 ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac tgatacatca
      241 gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa agattaataa
      301 accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg aacagttatt
      361 tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac ctaaggtgcg
      421 cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca agagggtcat
      481 tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt tctaagcagc
      541 ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa aaacaggtac
      601 gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag agcgcatcaa
      661 tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata acgtttcgat
      721 ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga cctctgacat
      781 ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc cggattatca
      841 gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg gccagtttga
      901 gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat acgataatca
      961 tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta tcgatcacga
     1021 ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat atctggtaca
     1081 gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc ttgttgccgc
     1141 gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc gtttttacga
     1201 cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg tgcgtacccc
     1261 gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg attccatcaa
     1321 cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg gcatcaacgc
     1381 cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc ataccggctg
     1441 tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg gcggtgtgcg
     1501 cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa gcctgctggt
     1561 gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact acggggtaca
     1621 aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc tgttcagccc
     1681 gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt ttgaacgcct
     1741 gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag ccgttgagct
     1801 gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc agaacgttga
     1861 ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc agtctaacct
     1921 gtgcctggaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg agaacggtga
     1981 aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc tggatgaact
     2041 ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt atcaggatta
     2101 cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta ttggtgtgat
     2161 taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca gcgccaacaa
     2221 cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt ctaatgagct
     2281 ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga aaggtatcct
     2341 gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc tgcattacga
     2401 ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca cgctttctgc
     2461 cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta ttgaaccgcc
     2521 gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg tggtgccgga
     2581 ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta acgatggcta
     2641 cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg ctaacaccaa
     2701 ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc tgaaagacct
     2761 gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc gtgacggcgc
     2821 tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg aaagcggcgc
     2881 atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg gcctacggct
     2941 cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg ccggatgcag
     3001 cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac gcgccagcgt
     3061 cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc ccaacaggac
     3121 acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa agaaccgatg
     3181 ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga catcttcgaa
     3241 aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga cgtctcccgc
     3301 gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat cagcaacctg
     3361 aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc gctattgccg
     3421 cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc agaaacgatt
     3481 cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc tgttgtgttt
     3541 gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc cagctattac
     3601 gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac ccacaccgtt
     3661 aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta tctctgcctg
     3721 atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg ttcttttgcc
     3781 tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat tgcccgcgat
     3841 gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag cggcgcggat
     3901 gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga cctgtttgtc
     3961 caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg ttcgatgatt
     4021 ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat ccgcatgcag
     4081 gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg gatcaacacc
     4141 tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag ttcttatctg
     4201 gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca gctctgatgg
     4261 cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa cacccttccc
     4321 ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc gaaggttact
     4381 gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc gaaccgttag
     4441 cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc gatattgaaa
     4501 tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat ctggcctaca
     4561 acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta gggatcggct
     4621 ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga cgcaagcggc
     4681 gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt ttcacataga
     4741 tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc ttaaacgcca
     4801 gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat actgcatgcc
     4861 gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc agcaacgcgc
     4921 gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg atgacaccgg
     4981 cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg ccgaacggat
     5041 aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt ttgccgttgc
     5101 gctcgacgca ttgcaccctg ctcataccat tacggcgata gttaggctct tcaacccagt
     5161 cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact gccataccct
     5221 atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagc aaaatcttaa
     5281 tgaaacgtgt cgtattccgc tacaggtgtg acaccgcttt tgcccttcat ttgcagcgca
     5341 tagattccac cagccactac cactaatagt gcgcccagca gataaaatgt gaatttaatc
     5401 agtcggatac agcgatgaat tttattcgac agggattagg catcgcctta caaccagagt
     5461 taacgctgaa aagcattgca ggtgaattgt gttccgttcc tcacgaacca accttctatc
     5521 gacagatttc gttgctgact aaagaaaagc cggtagaagg cagtccactg tttttactac
     5581 aaatgtgcat ggaacaatta gtggcgattg gaaaaatttg atatgagaca ggatggcgca
     5641 tgaacgccat cctgcagtaa cattactctt tattaagaaa ttttactgcc ttatcaggga
     5701 aatcagtaaa cagcccattt acacccgctt tgttatacag agcatcatat aactgattca
     5761 catcaggagt gtattcaggc agtttatctg accgcacggt ataaggatgc actaccagtt
     5821 tattctgctg agcatcttgc accatgccag tgagtttgat attacccggc tgcgatgtct
     5881 cctcaatcaa catatggtaa tccggaccaa taccatctgc atattccgcc acctgtttca
     5941 tggcacccgg cttaaacatc cagtcgtagt tgtaattaac ccagcttcca tccggctgtt
     6001 tctgctgcgt ttcattccag tcggtatagg caatcagctg taccagattg agctccatgc
     6061 ccattttggg ttccagctca ttcttaatac gcttcagctc atcagcatca aaacattgca
     6121 aataaacttt atcgtcttta ccggtgtaac catatttctt cagcacttcc agcgtttttg
     6181 ccgcaatatc cttcccttcc tgatgatgga accacggcgc tttgatttct ggatagatac
     6241 cgatattttt cccggtagag tgatttaacc cctgaacaaa ttcaatctct tcttcaaagg
     6301 tgtgcacccg gaagtcggac ttacccattg ggaaacgccc cggataagtc tgtacttttt
     6361 taccgttttc aatatcgaaa ccttcggtaa atttcagcga cttaatttca tccagcgtga
     6421 aatctatcgc gtagtaacga ccgtctttgc gcgcccgatc cgggaaacga tcggcaacat
     6481 cagtaacacg atcgagataa tggtcatgca gaacaaccag atggtcgtct ttggtcatca
     6541 ccaaatcctg ctccagataa tccgctccct gcgcatacgc catcgctttt gctggcagcg
     6601 tatgctccgg caaatatcca ctggcaccgc gatgggcgat gactattttt tcgttgctgt
     6661 ccgccgccat tgcactgctt cccatgacta tagtgctcat catgatcgcc atgctaaggt
     6721 ttttcagctt caatttcatg ccattagcct ccgttgcgtt tttgcagtaa ttgttcatgg
     6781 cgacgttttt cgccaatcat cacaacaatc aacaagataa ccgccagaat gctgccgcca
     6841 atcattacca taaagccgcc atcccagccg aagaagtcca cggtgtagcc aacaatcgcg
     6901 ctcgccgcca ccgaaccgcc caggtaacca aacagcccgg taaagcccgc tgccgtacct
     6961 gccgcttttt tcggtgccag ttccagcgca tgcagaccga tcagcatcac aggaccgtag
     7021 atcaggaagc cgataacaat catacaaatc atatcgacgg ttgggttacc tgccgggttc
     7081 atccagtaaa cgatagtcgc gatggtcacc agtgtcataa agaagacgcc ggttgcccca
     7141 cggttaccac ggaagacttt gtccgacatc cagccgcaca gcagggtgcc cggaataccc
     7201 gcatactcat agaagaagta ggcccaggag gatttatcca gcgcgaaatg cttaacctct
     7261 ttcagataag tcggtgacca gtcgaggatg ccgtaacgca gcagataaac gaacacgttg
     7321 gcgatggcga tgtaccacag cagtttgttc ggcagcacgt actgcatgaa gatttgcttc
     7381 gcggtcagct cctgttccgc tttttcgtta tagtcgtccg gataatcatt tttgtactct
     7441 tcgatcggcg gcaagccaca ggattgcggg gtatcgcgca tcatcgcaaa ggcgaataat
     7501 gccaccagaa tggcgcagaa agcaggcata tagagcgccg catgccagtc attgaaccag
     7561 gccatcccca gcaggaacag cagcggcgga ataccaccac cgacgttgtg cgcacagttc
     7621 cacactgaca caatgccgcc acgttctttc tgcgaccacc agtgcaccat agtacgacca
     7681 cacggcggcc accccatccc ctggaaccaa ccgcagagga acaacagtac aaacatcacc
     7741 gcaatgctcg acgtcgccca tggcacaaag cccataaaca acatcactgc cgccgccaga
     7801 atcaaacctg cgggcaggaa aacgcgcgga ttcgagcgat ccgataccga acccatgatg
     7861 aatttcgaaa atccataagc aatcgagatc cccgaaaggg caaaacctaa atcaccgcgt
     7921 gagaatccct gctcaaccag ataaggcata gcaagcgcaa agttcttacg aaccaaatag
     7981 taagccgcat agccaaagaa tatccccagg aaaatttgcc agcgcaatcg acgataagtc
     8041 ggatcgatct ccgcggcagg taagcgcgct ttgtgtggcg ctggtttaaa aatactcaac
     8101 attgatagcc tccgtggccc gtggtcttat ttatgattaa cagcctgatt cagtgagaga
     8161 acctgccgtt tcttgagttg ccgcgatgtt aagaaaacat tcataaatta aatgtgaatt
     8221 gccgcacaca ttattaaata agatttacaa aatgttcaaa atgacgcatg aaatcacgtt
     8281 tcactttcga attatgatcg aatatgcgcg aaatcaaaca attcatgttt ttactatggc
     8341 taaatggtaa aaaacgaact tcagagggat aacaatgaaa actcgcgact cgcaatcaag
     8401 tgacgtgatt atcattggcg gcggcgcaac gggagccggg attgcccgcg actgtgccct
     8461 gcgcgggctg cgcgtgattt tggttgagcg ccacgacatc gcaaccggtg ccaccgggcg
     8521 taaccacggc ctgctgcaca gcggtgcgcg ctatgcagta accgatgcgg aatcggcccg
     8581 cgaatgcatt agtgaaaacc agatcctgaa acgcattgca catcactgcg ttgaaccaac
     8641 caacggcctg tttatcaccc tgccggaaga tgacctctca ttccaggcca cttttattcg
     8701 cgcctgcgaa gaagcaggga tcagcgcaga agctatagac ccgcagcaag cgcgcattat
     8761 cgaacctgcc gttaacccgg cactgattgg cgcggtgaaa gttccggatg gcaccgttga
     8821 tccatttcgt ctgaccgcag caaacatgct ggatgccaaa gaacacggtg ccgttatcct
     8881 taccgctcat gaagtcacgg ggctgattcg tgaaggcgcg acggtgtgcg gtgttcgtgt
     8941 acgtaaccat ctcaccggcg aaactcaggc ccttcatgca cctgtcgtgg ttaatgccgc
     9001 tgggatctgg gggcaacaca ttgccgaata tgccgatctg cgcattcgca tgttcccggc
     9061 gaaaggatcg ctgctgatca tggatcaccg cattaaccag catgtgatca accgctgccg
     9121 taaaccttcc gacgccgata ttctggtgcc tggcgatacc atttcgctga ttggtaccac
     9181 ctctttacgt attgattaca acgagattga cgataatcga gtgacggcag aagaggttga
     9241 tattctgctg cgtgaagggg aaaaactggc ccccgtgatg gcgaaaacgc gcattttgcg
     9301 ggcctattct ggcgtgcgcc cgctggttgc cagcgatgac gacccgagcg gacgtaacgt
     9361 cagccgtggc atcgtgttgc tcgaccatgc tgaacgcgat ggtctggacg gatttatcac
     9421 catcaccggt ggcaaactga tgacctatcg gctgatggct gaatgggcta ccgacgcggt
     9481 atgccgcaaa ctgggcaaca cgcgcccctg tacgactgcc gatctggcac tgcctggttc
     9541 acaagaaccc gctgaagtta ccttgcgtaa agtcatctcc ctgcctgccc cgctgcgcgg
     9601 ttctgcggtt tatcgtcatg gcgatcgcac gcctgcctgg ctgagcgaag gccgtctaca
     9661 ccgtagcctg gtatgtgagt gcgaagcggt aactgcgggt gaagtgcagt acgcggtaga
     9721 aaatttaaac gttaatagcc tgctggattt acgccgtcgt acccgtgtgg ggatgggcac
     9781 ctgccagggc gaactctgcg cctgccgcgc tgccggactg ctgcaacggt ttaacgtcac
     9841 gacgtccgcg caatctatcg agcaactttc caccttcctt aacgaacgct ggaaaggcgt
     9901 gcaacccatc gcctggggag acgcactgcg cgaaagcgaa tttacccgct gggtttatca
     9961 gggactgtgt ggtctggaga aggagcagaa agatgcgctt tgatactgtc attatgggcg
    10021 gcggcctcgc cggattactc tgtggcctgc aactgcaaaa acacggcctg cgctgtgcca
    10081 ttgtcacgcg tggtcaaagc gcgttgcatt tctcatccgg ttcgctggat ttgctgagcc
    10141 atctgccaga tggtcaaccc gtggcagaca ttcacagtgg actggaatct ttgcgtcagc
    10201 aggcacctgc acatccttac tctcttctcg ggccacaacg cgtgctcgat ctcgcttgcc
    10261 aggcgcaggc attaatcgct gaaagcggtg cgcaattgca gggcagcgta gaactggctc
    10321 accagcggat tacgccgctc ggcactttgc gtgcaacctg gttaagttcg ccagaagttc
    10381 ccgtctggcc gctacccgcg aagaagatat gtgtagtggg gataagcggg ctgatggatt
    10441 tccaggcgca tcttgcggcg gcttcgttgc gcgaactgga tctttcggtt gaaaccgcag
    10501 aaatagagct gccggaactg gatgtcctgc gcaataacgc caccgaattt cgcgcggtga
    10561 atatcgcccg tttccttgat aatgaagaaa actggccgct gttacttgat gcgcttattc
    10621 ctgtcgccaa tacctgcgaa atgatcctga tgcccgcctg cttcggccta gccgatgaca
    10681 aactgtggcg ttggttgaat gaaaaactac cttgttcact gatgcttttg ccaacgctgc
    10741 cgccttccgt gctgggcatt cgtctgcaaa accagttaca gcgccagttt gtgcgccagg
    10801 gtggagtgtg gatgccgggc gatgaagtga aaaaagtgac ctgtaaaaat ggcgtagtga
    10861 atgaaatctg gacccgcaat cacgccgata ttccgctacg tccacgtttc gcggttctcg
    10921 ccagcggcag tttctttagt ggcggactgg tagcggaacg taacggcatt cgcgagccga
    10981 ttctcggcct tgatgtgcta caaaccgcca cgcggggtga atggtataag ggcgattttt
    11041 ttgcgccgca accgtggcag cagttcggtg taaccactga tgagacgcta cgcccgtcgc
    11101 aggcagggca aaccattgag aacctgtttg ccatcggttc ggtgctgggc ggatttgatc
    11161 ccatcgccca gggatgcggc ggcggtgttt gtgccgtcag tgctttacat gccgctcaac
    11221 agattgccca acgcgcagga ggccaacaat gaacgacacc agcttcgaaa actgcattaa
    11281 gtgcaccgtc tgcaccaccg cctgcccggt gagccgggtg aatcccggtt atccagggcc
    11341 aaaacaagcc gggccggatg gcgagcgtct gcgtttgaaa gatggcgcac tgtatgacga
    11401 ggcgctgaaa tattgcatca actgcaaacg ttgtgaagtc gcctgcccgt ccgatgtgaa
    11461 gattggcgat attatccagc gcgcgcgggc gaaatatgac accacgcgcc cgtcgctgcg
    11521 taattttgtg ttgagtcata ccgacctgat gggtagcgtt tccacgccgt tcgcaccaat
    11581 cgtcaacacc gctacctcgc tgaaaccggt gcggcagctg cttgatgcgg cgttaaaaat
    11641 cgatcatcgt cgcacgctac cgaaatactc cttcggcacg ttccgtcgct ggtatcgcag
    11701 catagcggct cagcaagcac aatataaaga ccaggtcgct ttctttcacg gctgcttcgt
    11761 taactacaac catccgcagt taggtaaaga tttaattaaa gtgctcaacg caatgggtac
    11821 cggtgtacaa ctgctcagca aagaaaaatg ctgcggcgta ccgctaatcg ccaacggctt
    11881 taccgataaa gcacgcaaac aggcaattac gaatgtagag tcgatccgcg aagctgtggg
    11941 agtaaaaggc attccggtga ttgccacctc ctcaacctgt acatttgccc tgcgcgacga
    12001 atacccggaa gtgctgaatg tcgacaacaa aggcttgcgc gatcatatcg aactggcaac
    12061 ccgctggctg tggcgcaagc tggacgaagg caaaacgtta ccgctgaaac cgctgccgct
    12121 gaaagtggtt tatcacactc cgtgccatat ggaaaaaatg ggctggacgc tctacaccct
    12181 ggagctgttg cgtaacatcc cggggcttga gttaacggtg ctggattccc agtgctgcgg
    12241 tattgcgggt acttacggtt ttaaaaaaga gaactacccc acctcacaag ccatcggcgc
    12301 accactgttc cgccagatag aagaaagcgg cgcagatctg gtggtcaccg actgcgaaac
    12361 ctgtaaatgg cagattgaga tgtccacaag tcttcgctgc gaacatccga ttacgctact
    12421 ggcccaggcg ctggcttaaa ctcccttctg atgcccggta agcatgtggt taccgggcat
    12481 ttttgcgtac acgattccgt gcccaatgta tgcgttgcaa cgcagtgaaa attcctctga
    12541 aaacgtctcg caaaggctga aactggcaga tgtcaaaggc ctgggataac cgtaatgtcg
    12601 cgtcatcata aatatcaggt gacggacaac catgaccgaa tcaacaacct cctccccgca
    12661 tgatgcggta tttaaaacct ttatattcac acccgaaacc gcacgggatt ttctcgaaat
    12721 acatttacca gaaccactgc gcaagctttg caacctgcaa accttacgcc tggaacccac
    12781 tagttttatt gaaaaaagtt tacgcgctta ctactcggat gttttgtggt ccgtggaaac
    12841 cagcgacggt gacggctata tctactgcgt gattgaacat caaagctctg cagaaaagaa
    12901 tatggctttt cggccaatgc gctatgccac tgccgccatg cagcgtcacc tagataaagg
    12961 ctatgacaga gttccgctgg tggtgccatt gctgttttat catggcgaaa catcgcccta
    13021 cccgtactca cttaactggc tggatgagtc tgacgatccg caacttgccc ggcagttgta
    13081 caccgaagct tttccgttgg tggatatcac catcgtacct gacgatgaga tcatgcaaca
    13141 tcggcgtata gctctgctgg aactgattca aaagcatatt cgcgaccacg atttaatcgg
    13201 catggtcgac aggatcacca cgcttttggt tagaggcttc actaatgaca gccagctaca
    13261 aacactgttt aattatctgc tgcaatgcgg cgatacctcc cgtttcaccc gttttattca
    13321 ggagattgcc gaacgttcac cactacaaaa ggagagatta atgactattg ctgaacggct
    13381 acggcaggaa gggcatcaaa ttggctggca ggaaggtatg catgaacaag ccattaaaat
    13441 tgccttgcgc atgctggagc agggctttga acgtgagatt gtgctggcgg caacccaact
    13501 cactgatgct gatattccga actgttatta aaaggagagg gagagattga tgactattgc
    13561 tgaacggcta cggcaggaag gacatcaaat tggctggcaa gaaggtaaat tagtaggttt
    13621 gcagcaaggt aaattagaag gtttgcagga aggtatgcat gaacaagcca ttaaaattgc
    13681 tttgcgcatg ctggaacagg gcattgatcg tgaccaagtg ctcgcggcca cccagctaag
    13741 cgaagccgat ctggcagcga ataaccacta attaacacag gcccacagcc gatccccatg
    13801 ggcctttgat atcaataact accttttatg cgtggcccat ttttgcctga tttaaacatc
    13861 gccagtcgtt gatccagggc atcgctgtag agcatcgtgt caacgccaac agcgacaaag
    13921 ttcgctcccc acgccaggca ttgctgcgcc atatcaggag ccacagccag aaaaccagcc
    13981 gctttacccg cagcacggat ccgccgaatg ctggtttcaa taattcgctg cacttccggg
    14041 tgcccggcgt tatccgggta gcccaacgac gcagaaagat ccgctggtcc aataaacacg
    14101 ccatcaatcc cttcgacgtc gaggatctcg tccaggttat ccagtgccgt tttactttcc
    14161 acctgcacta acagacaaag cgaatcgttg acttgcgcca tgtaattctc aatgcgtccc
    14221 cagcgcgcag cccgtgccac actggcccca acaccacgct caccgtaggg agggttggac
    14281 aaattttgcc ggagggggaa tgtgccggtg cagtaaaccg gcacagagtg gcgaaatcag
    14341 acggataaag tcatggactc aacaacctca atccagccat gctcactagc gatgtcctgg
    14401 ccgtttaacc aacggcgtag catattcagt gccatcattg cgcacacttc ctgacggata
    14461 gccaggctgt agcgtgtagt gctgaaacgc acacgcagag cgaaagtgcc gtctggcgtg
    14521 gctagcgcaa agttgagatg ctcgttctcg aaacccgaaa cagccagtgc cagcccggca
    14581 aaatggttgg cccgccgttc tgtaatccag tgcgcagttt gcgccagggt ttcttcctgt
    14641 gaaggaatca cttcacacgc cagcaatgga gcacctgcgc gagaaagttg caaagccaat
    14701 aaaccaccgg tgaattgctc gctcaacgtc aggctgaact ggcggttttg caattcgcga
    14761 ctgatctgcg cgggcagtcc ttcggtgcct tcgaaaatca cgctctgtcc ggcaacacgt
    14821 ttaacgtcca gccacagttt ttccatcgcc tgttgctcgc ttgccggtcc ggtgagtttc
    14881 agttcgatga tcggcattga ggagcgatag cccattgtta cgcccggcgg cagttgtaga
    14941 gtgtccaggc tttgtgccag atcgctttcc gaacgaccaa aggtagttaa acgcagacaa
    15001 accggcggct gtggtaaaga aaaacgctcg cgcaggcgcg gcaggatttc gtgctcgacc
    15061 atcaccttaa attctgacgg tacgccggga gtaaagaaca tcaggcaacg attaagctgc
    15121 acggcaaaac cacaggcggt gcctaccggg ttattgataa attcagcact ggcaggcagc
    15181 tccgcttgtt tacggctgct cggtgccatt actcgtccac gttcgtggaa atagcgttcc
    15241 atctctttga gccaggcttc atgcagcaca aggccttcac cttttgctgt cgcagcggcg
    15301 agtgcgctta aatcatcgct ggtcggtccc agcccgccgt taacgatcag cacatcggcg
    15361 tgctgactac gttcgcgaag aatggtgact aagtcatcaa ggttatcccc caccgtattg
    15421 cggcgagata atggcagccc ctgatgaaag aaaaaatcgg ccagccaggc agcgttagtg
    15481 tcaacgattt gcccgtataa cacttcatcc ccggtggata gcatttccac ttttaacatg
    15541 gttatctcct gcttttgtta tcgaatcact ataacgcaaa gggagataag gcgtagtaag
    15601 aaactgaatg ccgcgccgga agggatcaga aactggcgtt aaccccaaca tacaggccgt
    15661 cagccacagc gttgtcgcgg ttaccgtctt tacccgacag attcaggtag cgataacccg
    15721 cctcaatact gactggacgc ataatggtgt aacgcgcgcc agcattcgct tcctcataac
    15781 tttgaatacc gctggagagc gaatccggag agtagtaata ctcgccaaac aaacggaagc
    15841 tgttgccaat tttccactgc aaaccacctc ctaccgctgc ggcataacct tcatcgccgt
    15901 aattcgggtt ggtgtacacg ccttttccgc caacggtcgc cattaacggc ccgagaggca
    15961 gattcaaccc cagccccacg cccgcgacgt caccgtcgtc gtcgttatgt gtccagttac
    16021 cgcttaaagc caggcccgtc gattcagtcc caaaaccgac accaatattg gtgtattctt
    16081 tacccgcctg gccgctgatg ctcattgcat tgaccgatgc agaaaccaac agcattccgg
    16141 ccagacctgc tagcgcaatt tttttcatta tcccctttcc cacaagcaat taatcaaaat
    16201 tcccaaagct gcgggattgt actgcctggc acagaggatt caatgagcga aaaaagggat
    16261 ctagtcactt attgtaacta aatacttctt aaataattaa acgccgaaaa aataaacaca
    16321 ctttaccgat acacacaaaa ataataataa gtgcagttat cattgcaaaa gagattactg
    16381 tcactttcac atatttctga gttaccgtat tctcatggtt tctttttatt tataaggaat
    16441 acccctgtgc gacaacggac tattgtatgc cctttgattc aaaatgatgg tgcttatttg
    16501 ctgtgcaaaa tggccgacga tcgcggcgtt ttccccggtc aatgggcgct ttcgggtggc
    16561 ggcgtggagt ctggcgaacg aattgaagag gcactacgcc gcgaaattcg cgaagaactg
    16621 ggagaacagc tgcttttgac agaaatcacg ccgtggacct tcagcgatga tattcgcacc
    16681 aagacatatg cagatggtcg caaggaagag atttatatga tttacctgat ttttgactgc
    16741 gtttctgcca accgagaagt gaaaataaac gaagagtttc aggactacgc gtgggtaaaa
    16801 cctgaagatc tggtgcatta tgatttaaat gttgccaccc gaaaaacgtt acgtttgaaa
    16861 ggtcttcttt aacaatgatg acagccatca ctggctgtca ggttgaaaat ttagtggtta
    16921 acgaattccc catccagata aactttgcct ttttccacat gcatgactaa accatccaga
    16981 taatcaggtt taaatatcgc atcacgctta tttttagcaa tatatgtcag gcaatgatta
    17041 tgggtgaaaa taaccatatt tttatcaggc gctttgcttt gtaagtcctt aattgcacta
    17101 taaatctcat taccgcactg aagaagtcgt ttatctaccg tcagcttttt acccgcggaa
    17161 aaccaggtcg ccgactgaat ggtccggacg gtattactgg aataaagatc gaaatcaggg
    17221 atgtcagcgc taaaagcgtt gcccagttca cgggcatcct gggtaccttt aaccgtaata
    17281 cctgttttat ctgacaagca ttgattggtt gaacgatcgc aacgttcagc atggcgaaac
    17341 aaaacgacaa ccgggtgctg ctgtgccagt ctggccaatg ttttattgtc gatacgtggc
    17401 aaaccattgc tactccaggc ggcatgagta cccagtccgg caattgcagc gagcgccagt
    17461 aaaatgatga aatatttttt tgacttcaat gatgagcggc aaaaagctaa cacgtaaact
    17521 ccacctgtag acaagcgcaa ccagacaatt accgtgaaat tgagcgacat ttctggcgat
    17581 aattagcagt tggtgtaata ttaaaaatcc tacgatgtcg gcaaaatgcc tcaaattttt
    17641 gccaaatgca aagcctaaat aagaaaaaat ataaaaattt caatatttac gtctaatatt
    17701 agtttcttaa ggttaagtta atattctatc cttaaaattt cgctccaaat ggcaaaatat
    17761 acacaacact ctttatagca aatataagtg gacaggtatt caatggcgga aggaaaagca
    17821 atgtcagaat ttttgccttt ttcgcgacca gcaatgggcg tggaggaact cgctgcagtt
    17881 aaagaggttc tcgaatccgg ttggatcaca accggtccga agaatcaggc gcttgagcaa
    17941 gccttttgcc agttgacggg aaatcagcat gccatcgcgg tcagttcagc caccgccgga
    18001 atgcatatca cgctaatggc gttggaaatt ggcaagggcg atgaagtgat tacgccttcc
    18061 ctgacctggg tttcaaccct caatatgatt tccttgctgg gtgcaacgcc ggtaatggtg
    18121 gatgtcgacc gcgatacgct gatggtcacg cctgaagcga tcgaatcagc cattacgcca
    18181 cgcactaaag ccatcattcc ggtgcattat gccggtgcgc cagcagatat tgacaccatt
    18241 cgcgccattg gcgaacgtta cggcatcgca gttatcgaag atgctgccca tgccgtcggt
    18301 acgtattaca aagggcgaca tattggcgca aaaggtaccg ctattttttc atttcatgcc
    18361 attaaaaata ttacctgtgc tgaaggtggc ctgattgtaa ctgataatga aaaccttgcc
    18421 cgccagctac ggatgctgaa atttcacggt ctgggtgtcg atgcctatga cagacaaacc
    18481 tggggccgtg caccgcaggc tgaagtctta acaccgggct ataagtacaa tctgaccgat
    18541 attaacgccg cgattgccct gacacagtta gtcaaattag agcacctcaa cacccgtcgg
    18601 cgcgaaattg cccagcaata tcagcaagca ctggcagctc tcccctttca gccattaagc
    18661 cttcccgcct ggccgcacgt tcacgcctgg catctgttta ttattcgtgt cgatgaacaa
    18721 cgttgtggta tcagtcgcga tgcgttgatg gaagcgttaa aagaaagagg cattggtacc
    18781 gggttacatt tccgcgccgc tcacacacaa aaatattatc gcgagcgttt tcccacgctg
    18841 tcgttaccga ataccgaatg gaatagcgaa cgcatctgtt cgttgccgct gttcccggat
    18901 atgactaccg ccgatgccga ccgcgtcatc acagcccttc agcaactcgc aggacaataa
    18961 gccatgtttg aaatccaccc tgttaagaaa gtctcggtgg ttattcccgt ttataacgag
    19021 caggaaagct taccggaatt aatcaggcgc accaccacag cctgtgaatc gttggggaaa
    19081 gagtatgaga tcctgctgat tgatgacggc agtagcgata attccgcgca tatgctggtc
    19141 gaagcctcac aagcggagaa cagccatatt gtgtctattt tgcttaaccg caattacggg
    19201 caacattcag cgattatggc gggattcagt cacgttactg gcgacttaat tattaccctt
    19261 gatgccgatc tccagaatcc gccagaagaa atcccccgcc tggtggcaaa agccgatgaa
    19321 ggttacgacg tggtagggac tgtacgccag aaccgccagg acagctggtt tcgtaaaaca
    19381 gcttcgaaga tgattaaccg gcttattcag cgcaccactg gcaaagcgat gggtgactac
    19441 ggttgtatgc tgcgcgccta tcgccgtcat attgtcgatg cgatgttgca ctgccatgaa
    19501 cgcagcacct ttatcccgat tctggcgaat atcttcgccc gccgtgcaat tgaaattcca
    19561 gtacatcatg ccgagcgtga gtttggtgaa tccaaataca gttttatgcg cctgattaat
    19621 ttgatgtacg acctggtgac ctgccttacc acaacgccgc tacgtatgct gagtctgctc
    19681 ggcagcatta ttgcgattgg aggttttagc attgcggtgc tgctggtgat tttacgcctg
    19741 accttcggac cacaatgggc ggcagaaggc gtctttatgc tatttgccgt gctgtttact
    19801 tttattggcg ctcagtttat cggcatggga ttactcggtg aatatatcgg caggatctac
    19861 accgatgtcc gcgcccgccc ccgctatttt gttcagcaag ttatccgtcc atccagcaag
    19921 gaaaatgaat aatgaaaacc gtcgtttttg cctaccacga tatgggatgc ctcggtattg
    19981 aagctctgct ggctgccggt tacgaaatta gcgccatttt tacccatacc gataatcccg
    20041 gtgaaaaagc cttttatggt tcggtggctc atctggcggc ggaaagagac attccggttt
    20101 atgcgccgga taacgttaat catccgctgt gggtggaacg cattgcccaa ctgtcaccag
    20161 aggtgatttt ctctttttat tatcgccatc ttatttgcga cgagattttt cagctcgctc
    20221 ccgcaggtgc atttaatctg cacggttcgc tgttaccaaa atatcgtggt cgcgcgccgc
    20281 tgaactgggt gctggtgaac ggtgaaacgg aaacgggcgt tacattgcac cgaatggtaa
    20341 aacgtgccga tgctggggcc attgtggcgc aactgcgcgt tgccattgcg ccagacgata
    20401 tcgctattac gctgcatcat aaattgtgcc atgccgcgcg ccagctactg gaacagacat
    20461 tacccgccat taaacacggt aatattctgg aaatcgccca gcgcgaaaac gaagccacct
    20521 gttttggtcg cagaacgccg gatgacagtt tccttgaatg gcataaaccg gcatccgtac
    20581 tgcacaacat ggtacgtgcc gttgccgatc cgtggccggg tgccttcagc tatgttggca
    20641 atcagaaatt caccgtctgg tcgtcgcgtg ttcatcctca tgccagcaaa gcacagccgg
    20701 ggagcgtgat ttctgttgcg ccactgctga ttgcctgtgg cgatggcgcg ctggaaatcg
    20761 tcaccggaca ggcgggcgac ggcattacta tgcagggctc gcaattagcg cagatgctgg
    20821 gcctggtgca aggttcacgc ttgaatagcc agcctgcctg caccgcccga cgccgtaccc
    20881 gggtactcat cctcggggtg aatggcttta ttggcaacca tctgacagaa cgcctgctgc
    20941 gcgaagatca ttatgaagtt tacggtctgg atattggcag cgatgcgata agccgttttc
    21001 tgaatcatcc acattttcac tttgtcgaag gcgatatcag tattcattcc gaatggattg
    21061 agtatcacgt caaaaaatgt gatttcgtct tgccgctggt ggcgatagcc acgccgattg
    21121 aatatacccg caacccgctg cgcgtatttg aactcgattt tgaagagaat ctgcgcatta
    21181 tccgctactg cgttaagtac cgtaagcgaa tcatcttccc gtcaacttca gaagtttatg
    21241 ggatgtgtag cgataaatac ttcgatgagg accattctaa tttaatcgtc ggcccggtga
    21301 ataaaccacg ctggatttat tcggtgtcaa aacaattact tgatcgggtg atctgggcct
    21361 atggcgaaaa agagggttta cagttcaccc tcttccgccc gtttaactgg atgggaccac
    21421 gactggataa ccttaatgca gcgcgaattg gcagctcccg cgctattacg caactcattc
    21481 tcaatctggt agaaggttca ccaattaagc taattgatgg cggaaaacaa aaacgctgct
    21541 ttactgatat ccgggacggt atcgaggcgt tgtaccgcat tatcgaaaat gcgggaaatc
    21601 gctgcgacgg cgaaattatc aacattggca atcctgagaa cgaagcgagc attgaggaac
    21661 tgggcgagat gctgctggcg agcttcgaaa aacatccgct gcgccatcat ttcccaccgt
    21721 ttgcgggctt tcgcgttgtc gaaagtagca gctactacgg caaaggatat caggacgtag
    21781 agcatcgtaa accgagcatc cgcaatgccc accgctgcct ggactgggag ccgaaaattg
    21841 atatgcagga aaccatcgac gaaacgctgg atttcttcct gcgcaccgtt gatcttacgg
    21901 ataaaccatc atgaccaaag taggcttacg cattgatgtc gatacctttc gtggcacccg
    21961 tgaaggcgtg ccgcgtctgc tggaaatctt gagtaagcat aatattcagg ccagcatttt
    22021 tttcagcgtc ggcccggaca atatgggccg ccatctctgg cgactggtga agccacagtt
    22081 tttgtggaag atgctgcgct caaacgcggc atcgctttat ggctgggata ttttactggc
    22141 aggtacggcc tggccaggta aagagattgg tcatgccaat gccgatatca ttcgtgaagc
    22201 ggctaaacat cacgaagtcg gtctgcacgc ctgggatcac catgcctggc aagcccgtag
    22261 cggtaactgg gatcggcaaa caatgatcga cgatattgca cgcggtcttc gcactctgga
    22321 agagattatc ggtcaaccgg taacctgttc tgccgctgcg ggctggcgtg ccgaccagaa
    22381 ggtgatcgaa gcaaaagaag cgttccattt gcgctacaac agcgattgtc gtggggccat
    22441 gccgttccgt ccactgctcg aatcaggaaa ccctggcact gcgcaaattc cggtgacctt
    22501 acccacctgg gatgaagtga ttggtcggga tgtgaaagca gaagatttta acggttggtt
    22561 actcaaccgc atcctgcgag ataaaggcac gccggtttat accattcatg cagaagttga
    22621 aggctgcgct tatcagcata attttgtgga tctcctcaaa cgcgcagctc aggaaggcgt
    22681 gacattttgc cctttaagcg aactgttatc agagacgttg ccgctcggac aagttgttcg
    22741 cggaaatatt gccggacgtg aaggctggct gggttgccaa caaattgcgg gtagtcgctg
    22801 atgaaatcgg tacgttacct tatcggcatc ttcgcgttta ttgcctgcta ttacctgtta
    22861 ccgatcagca cgcgtctgct ctggcaaccc gatgaaacgc gttatgcgga aatcagtcga
    22921 gaaatgctgg catccggcga ctggattgtg ccccatctgt tagggctacg ttatttcgaa
    22981 aaacccattg ccggatactg gattaacagc attgggcaat ggctatttgg cgcgaataac
    23041 tttggtgtgc gggcaggcgt tatctttgcg accctgttaa ctgccgcgct ggtgacctgg
    23101 tttactctgc gcttatggcg cgataaacgt ctggctctac tcgccacagt aatttatctc
    23161 tcattgttta ttgtctatgc catcggcact tatgccgtgc tcgatccgtt tattgcattc
    23221 tggctggtgg cgggaatgtg cagcttctgg ctggcaatgc aggcacagac gtggaaaggc
    23281 aaaagcgcag gatttttact gctgggaatc acctgcggca tgggggtgat gaccaaaggt
    23341 tttctcgccc ttgccgtgcc ggtattaagc gtgctgccat gggtagcgac gcaaaaacgc
    23401 tggaaagatc tctttattta cggctggctg gcggttatca gttgcgtact gacggttctc
    23461 ccctggggac tggcgatagc gcagcgggag cctgacttgt ggcactattt tttctgggtt
    23521 gagcatattc aacgctttgc actagatgat gcccaacata gagctccgtt ctggtactac
    23581 gtgccggtca tcattgccgg tagcctgccg tggctgggat tactccccgg tgcactgtac
    23641 acaggctgga aaaaccgcaa gcattccgca accgtctatt tgttgagctg gacgataatg
    23701 ccgctgctgt ttttctccgt cgctaaaggc aaattgccca cctatattct ttcttgcttt
    23761 gcacctctgg caatgctgtt ggcgcattac gctttgctgg cagcaaaaaa taatcctctg
    23821 gcgctgcgga ttaatggctg gattaacatc gcttttggcg tcactggcat tattgccaca
    23881 tttgtgatct ccccctgggg accaatgaac acgccggtgt ggcaaacctt cgagagctat
    23941 aaagtctttt gtgcctggtc gattttttcg ctatgggcat ttttcggctg gtacacctta
    24001 acaaacgtcg aaaagacctg gtctttcgcc gcgctttgcc cgctggggct ggcgttgctg
    24061 gtaggatttt caattcctga cagagtcatg gaaggaaaac atccgcaatt ttttgtcgag
    24121 atgacacaag aatcacttca gccaagccgc tatattctta ctgatagcgt cggtgttgcc
    24181 gcaggtctgg catggagcct gcaacgcgat gacatcatca tgtatcgcca gacaggtgag
    24241 ttgaaatacg gccttaatta tccggatgcg aaagggagat ttgtcagcgg tgatgagttc
    24301 gcaaactggc ttaatcaaca tcgtcaggag gggattatta cactcgtgct ttcggttgac
    24361 cgcgatgaag atatcaacag tctcgccatt ccgcccgcag atgccatcgt tcgtcaggag
    24421 cgtctggtgc tgattcagta tcgtcccaaa tgatctggct aacattagtc tttgccagct
    24481 tgcttagcgt tgccgggcag ttgtgtcaga aacaggcaac ctgctttgtg gcgataaaca
    24541 aacggcgcaa acatatcgtg ctgtggctgg gactggcgct ggcttgtctt ggtcttgcca
    24601 tggtgctctg gctgctggtc ttgcagaacg taccggtagg cattgcctac ccgatgttaa
    24661 gtctgaattt tgtctgggtg acgctggctg cagtaaaact gtggcacgaa ccggtatcgc
    24721 cgcgtcactg gtgtggggtg gcgttcatta ttggtggcat tgtgatcctc gggagtacgg
    24781 tgtaatgggc ctgatatggg gattattcag cgtcattatt gcctcagttg cgcaattaag
    24841 cctgggtttt gcggcaagtc atctgccgcc gatgacgcac ttatgggatt ttatcgcggc
    24901 tctgttagct tttggcctgg atgcccggat actgttactc gggctgctgg gttatctgct
    24961 ttccgttttt tgttggtata aaacgctaca taaacttgcg ctaagcaaag cctatgcctt
    25021 gctcagtatg agttatgtgc tggtatggat tgcttcaatg gtacttcccg gctgggaagg
    25081 gactttttcg ctgaaagcac tactgggagt agcctgtatt atgagcgggt tgatgctgat
    25141 tttcctgccc acgacaaaac aacgttactg agttttccct gccgctgtac actgccgttc
    25201 ccactcatcg gggctataac tgcttgccga gaggactttc accgtatgca gcttttcacg
    25261 attaatacaa taaagcgcat tctgcaaagg cgagagtaaa tcccccactt tcacggcgaa
    25321 gtcactcttc acttcggcga tcatttttat cgcgccgcca gcatcgcaga gcataagtac
    25381 atgtctgtta tcttgtttat tgcagcacga ttttttgtgc cagccattcc attgcattat
    25441 cctgtttgct aagagttttc acatcaattt acagattaac agataaactc tttatcaacg
    25501 gcaatggaat ggcagaaatg agaagtgaaa cggagggtta attattgctg acgttgcacc
    25561 cactctttta gcgcctgacg tgaaatttta ataccgccgt ttttcagctc cggcggtaaa
    25621 gtcagccagc gcaccggttg ttgaaaacga gccagcttat ctttcaccca ttcgccaaga
    25681 tcaacggttt gctggtcata ctccaccacc gctaccggcc gatgaccaaa ctccttgtcg
    25741 gcaacggaaa cgataaacac ctgcaaaacc gcaggatgtg cagcaataac acgctcgact
    25801 tcttccggct gaataccctc accgccactg aagaacagat tgtctaaacg tccgacaatg
    25861 gtcagcttgc cattatgcat ctcaccgcga tcgcgcgtag cgtaccagcc ttcgtcatta
    25921 accagtgaaa ccagttgccc gttacgccag taaccttctg ccatactggc agcccgcagc
    25981 cacacttcat tattaacgat tttcacttcc cgacccggca gcggcaaacc aacgtctgcc
    26041 aggacgtcgg cttctttcgc acataccgtg gaggcaaact cggtcagacc atagccgcaa
    26101 aagcaacgaa tcccctgctc gcgcgcctgt tccgtcaact cgaccgggat agccgcgccg
    26161 ccaagtaaca ccgctttcag ggaaacggaa ctgcggttaa ccagcaaacg ccagagttgt
    26221 gttggcacca gtgaagcgtg agtacagcct gccagcattt gctccaatgg ctgtttatca
    26281 cgcaccgtca tccgcgcacc agcgtataac cagcgccaca taattccctg accggagacg
    26341 tgaaacagcg gtaaagagag caaccagtca tcgtgatcgc caaacggaat cagcgataac
    26401 acaccttgcg cactggcaag atgggcctga taagtatgga cagcggcttt cggcaaaccg
    26461 gtagaacccg aggtcaacgt cattgagcac agacgcgtcg gctgccacgc agcggcatgt
    26521 acgccttcaa ccagctgaat gtgcagcgac gttaatgccg gaaacgtgtt ttccccatcc
    26581 ggcaccagcg caaattgcag cgtcagattg ggcagcaatt cttcaagcaa cggttgcggc
    26641 agctgagggt tcacgggcaa cacccgcgcc ccgcattgca gtaacgccag ccaggcgagc
    26701 agcgtttgcg gcgtattcca cgcccgcaac atcacgccgc tgccctcaac cacaccctgc
    26761 gccgcaaagc ccgtagctaa ctcatcgacg cgagcacaaa gctcgcgcca gttgagttgc
    26821 tcgtcattaa gacgtaaggc gatggcttct ccccgcactt gccgccagtg acgccacggc
    26881 cagtcagaga agatcataac aactgctcca gtacatcagc gtccactaac ggcagtgggc
    26941 tccccggcca gcgacgaacc tgctgcgcct gcatcagatc cagcgtgtcc agtcccggaa
    27001 tggtgtctgg cgttaaccag gcagcaatcc gcgccagttg cgttagacct aagctcgact
    27061 caatggaaga actgatcacc gccgtcagcc ccagcgcatg cgccgcctgt acctgctcgc
    27121 gtactttttc cagactgccc gtgagcgtgg gtttgataac taccgcgcgc acgccctctt
    27181 cagccacaaa ggcaaaatcc ggctcgcgca ggctttcatc ccaggcaatg gcaatgccgg
    27241 tttcacgggc aaacgctcgc gaatcatcgc gggttttgca cggctcttcg agaaacgcga
    27301 tgcggtggcg ataatccggg ttaacatatt tggcaaactg ctgacctttc agcggtgtcc
    27361 aggcgcgatt tgcgtcaaga cgcaaatgca gatccggaat tgcctccagc aacagattca
    27421 ccaccatgcc gtcgcgcacc gcttcgtaca atcccacttt gaccttcgcc actttctcgc
    27481 ccggcatatc ggcaagtttg aggatcagat cgtccggatc gccattacac agcggtgccg
    27541 cacggtagtt ggctgcttgc ggcaacgtat ctgtcagttc tgccaatgca cagcttacgc
    27601 caaaggccac ggaaggcatc tgcggtagct cgcaatcgcc tgccagccag ttatttaccc
    27661 aggcaagcag cacgctttgc gcctcttccc agttttcctg gctgaagccc ggcagtgggg
    27721 agatctcccc ccacccttcg cgctcgccct cacgcaggca aacgtacagc ccgtcgcggg
    27781 tttttaaccg cctgtcgcgc agaagcaccc ccgcgtccat ggggatctgc cagcggtata
    27841 cctgcgcgct acgcattacg gattccgttt aaatttgctg aaatcaggct gacgtttctg
    27901 gttgaaagcg ttgcgacctt cctgaccttc ttccgtcatg tagaacagca tggtggcgtt
    27961 gcccgccagc tcctgcagcc ccgcctgccc gtcacagtcg gcgttcagcg cagctttcag
    28021 gcagcgcagc gccatcgggc tgttttgcag catttcgcgg caccaacgga cggtttcttt
    28081 ttccagatcc gccagcggta ccacggtgtt gacaaggccc atatccagcg cctgttttgc
    28141 gtcgtactga cggcacagga accagatttc tcgcgctttt ttctgcccga cgatacgagc
    28201 catgtaggaa gcgccccagc cgccgtcgaa ggaaccgact ttcgggccag tctgaccgaa
    28261 gatggcatta tctgctgcga tagtcaggtc gcacatcatg tgcagaacgt gaccaccgcc
    28321 gatggagtag ccagccacca tcgcgacaac cggtttcgga caggtacgga tctgacgctg
    28381 gaagtccagc acgttcaggt gatgtacgcc ggaatcatct ttatagccgc cgtaatcacc
    28441 acgcactttc tggtcaccac cggagcagaa cgctttatcg cctgcgccag tcagaatgat
    28501 cacgccgata ttgtcgtcat aacgcgcatc tgccagtgcc tggatcatct cttttaccgt
    28561 cagaggacgg aaggcattgc gcacctgcgg acgattaatg gtgatttttg cgataccgtc
    28621 ggtggatttt tcataacgaa tgtcctcgaa accttcggag cagtcgtgcc attcaaccgg
    28681 tgcgtaaggc attgcttcat caggataaat catattgtgt cctttagtca aaaacgcaag
    28741 atctgcgcca gacttgcgat tacgccagcg ggattttccc gatgcgcgtt atgtccggcg
    28801 cgaggaatga catggcagtc ggcagccagt tccgccgcta gggcgcggaa tttgctgtca
    28861 cgttcaccac ataaataata aaacgcaaat gtgcgggcgc taaggttagc acgtaaatca
    28921 ggctggacgg cgagagaagt cgcctccagc atggcggcaa gggttgcgcc attattgttg
    28981 ctgcgcagcg ccaccagctc ccggcgttga tcgtcattga gcgaggcaaa aacaggctgt
    29041 tgataccagt cggcaaatac cgccgttaac ggttctgtgc gaaaacgctg cgcccattgg
    29101 cgatcggaac gctgacgttc cgcacgttgt tcagcatttt gcagccccgg atgcccgcct
    29161 tcgataataa ccccacaaag ccccgccagc ccctggcaag ccgccatcat cgccacccgt
    29221 ccgccaagcg agtaccccac cagccagaag tcaaggatgt tgtaactaac caaggtttta
    29281 cgcagtaagt cggtgacatc atcaaatcca tcgacgctaa tcgccgccga accaccatga
    29341 cctgggagat caacatacaa ccgtgagtag tcggcaaacg cctcgcccac ttcttgccat
    29401 tcgtggcaat cgccggaaaa accgtgcaaa aacaccagcc agggtaaacc tggttttccg
    29461 tgttttgcct gcgcgtgcag gatcataaat ggcttacctg cgccagaagt tgctggagcg
    29521 tttgcgcacc atcggtgtcg ttaaccacca tttcaatcac cgtggtggtt ggcgtgcgcc
    29581 aggcgtcggc aaatgccgtt tcaagttcct gccagttttg cggacgatga tatttcagct
    29641 cgaacatcgc ggcggcgtgc tcaaaatgga cgttttgcgg catcagatag aaacgctcac
    29701 gctcgctttg cggcgttggc aacagcgaga aaatttgccc gccgttgttg ttcaccacaa
    29761 ttaataccag cggcgcagaa acctgacgca ataacgccag cgcgttgaga tcgtaaagtg
    29821 cggagagatc gcccacaatc gccagcgtcg gtttgccgct tgcccgctga acgcctgcgg
    29881 cggtcgaaag cagtccgtcg ataccgctgg cccctcggtt gctgtacacc gggtaacctg
    29941 ccggaagttg cgaaagcgca tcaatcagac gtaccaccag gctgttacca acaaacaatt
    30001 gcccctgttc aggcagatag tcgctgatgc gatgcgccag ttgcgcttcg ccaaacgcat
    30061 cacggcgggc aataaccgcc tgcattgcct gttccgccag gcgcgggatt tcaacgcacc
    30121 agggctggcg tttttctgcc ggatgcagct ccagccagtc ggcaatattg ccaattaagc
    30181 gacgtccgcg atggtgtgcc ggatcaagtc gcccttcaat gtcatcaaca atccagtact
    30241 cttctggttc acagcttgcc tgccattgca ggagccgttt ccccgtcagg ctgcttccca
    30301 gttgcaccac aatttgtgcc tgctgcagct cgctggtcgc tttggcattg cctaaccaaa
    30361 gatcggcaca cggcagcggc tgcccggttt gtgacagcac atcgccaatc agcggccagc
    30421 caagagtttg cgcccacagg gcaacttttt tgccctcttc cgcactcatg cgcccggcaa
    30481 ccaccacgcc gcgcttttgt cgccagaaga accagtcgcg ctgtttttca ctttccagac
    30541 gaggcgcttc acgcagccac ggtttgtcgt cctgccacca gtcacccaga cgctgttgcc
    30601 agctaagccc ggtatcgtcc atttcgccat acagcggttc agcaaacggg cagttgatat
    30661 ggactccccc cgcatgaagc gtaccgagag cgtggtcgat ggtagaaacc agccaacgtg
    30721 cggggatatc ctgggtcggg cgcggcaatg aaatactgtg cgtggggtga gaggcgaaca
    30781 ttcccggctg gcgaattgcc tgattcgcgc cgcagtcaat tagctccggc gggcgatcgg
    30841 cggttaagag aatcagtttt tctccggtta acccggcttc aatcagtgcc ggatagagat
    30901 ttgccaccgc cgtgccggag gtcacaatca ccgccaccgg ctgcttgctg actttcgcca
    30961 gccccagcgc cagatgcccc aacccacgct catcgaaatg ggtgtggtga atgaatgcgg
    31021 aattctccgc cgccgctaac gttaacggtg tagaacgcga gcctggggcg atacagatgt
    31081 gtctgacgcc gtgacgcgtt aatgcttcca gaatgaccgc cgcccagcgt cggttaaatg
    31141 cgcttactga catgagtttg tccggtatca atattgcggc taagtataag gagctacaaa
    31201 aatagattat tgatatgaat cggtaatgat gcgactcatt actattccat ttgtaataaa
    31261 gtacgcagcc ctgccgcttt gttgtcgatt tcctgccact cttgctcggg gtcggaacca
    31321 cggacaatgc ccgcgccagc atataatcgc acgacattgc cgctaatttt tgctgagcgc
    31381 agggaaacgc agaattcgct ttgttgtaat gagagatagc ccgcagaacc ggcgtaccat
    31441 tctcgggtga acggttcgtg acgggcgata aactgtcgcg ccagatcgcg cggtaagcca
    31501 gcaactgctg ccgttggctg caactgatgt aaacagatca catcatccgc tttgttgagt
    31561 gaagtccaga tacagcggcg aagatgctgc actttacgca gacgcagtac ctgcggcggt
    31621 aaaacatcca gcgtctgggt atcggcctgt aatcgttggc agatatcttc caccaccagc
    31681 atgttctcgc gctggttttt atcatccgcc atcagccact ctcctaactg ctgcgcctgc
    31741 ttatcatcag gatgatttgc tactgttccc gccagcgctt cagtacgcag cgctttgtca
    31801 cgccgccgcc ataaccgttc cggtgaagag ccaagaaaag cattttcgcc atcaaaggcc
    31861 atgtaaaaat ggtagcaatt cagattcagt cgacgactgg cagccatcat cgccgccgcg
    31921 ttgaccggac ttgcgaaatg caggtcagtt gcccgagcga gcaccacttt gtcgagctca
    31981 ccttcggcga tggttttcgt tgccagttcg attaattgcg tccagcccgt tttgtccggc
    32041 cagtgttgtt ctcgcgtggt ggttaaatgt aacccaggca agggcttgat actcaccagt
    32101 gtggcgataa attcttttgc ctgaatcgca tcgtgctgaa gggagctttc gctgaatagc
    32161 gtcaaccgca gcgtggcttt accgccacag cgtcgccatt ccaggcgggg taaaagtaaa
    32221 ttgccctgcg acgggtcaaa agcattcagc ccccaaatgc gtaagtcggc gtgttccggg
    32281 tgctggcgaa gaaaacgttg tgcctggtcc aacgacgtaa aacgggtaat cgcgcccagg
    32341 acggcagctt cttcatcacc attacgttgt tgccagtaga attgcgggta cgtttgctga
    32401 ctggccagcc agctcaaggc atcaaaagcg tctttgagag ggaaaggaat atcgataacc
    32461 cgaatgccgg gtgtcgccgg aatctcttgc gacaaatggc gcagtagatt ttccagcgcc
    32521 gtagtaagtg attgcacgcg gacctctccc tgttaaaagc ctcacattat acggggtact
    32581 acaaaaaaaa tgcagtaccc cggtgtaggg aggttttaac ggcgtgccag caacagtcct
    32641 agtaccagcc cgacggccgc gcccacacca attccttgcc agggtttttc gtggacgtag
    32701 tcatcagcac gataaacagc ctgcttcgcc cgatagtaat aactgtctga agcctggcta
    32761 acccgttttt tcacatcatc cagcgctttt tctgcacgcg ctttcagctc aacatatttc
    32821 tgatcggcgg gctcgccaga ggagcggagc acctcttcca gtgtttcact aagcagcgtc
    32881 aggtcgtcat cgatacgtgt atcaccaaac tgattagaca ttctcgttct ccatttgcgt
    32941 aaaacctgtc actaactata gacaagagtt gttgattacg cctgaattac ctcgcgtgcc
    33001 atcccaatgt gtgggatacc atcttcttca tagacctccg tcacagggat aaaaccgaaa
    33061 ctctggtaga agttttgcag atgcgcctgc gccccgaggt aaacaggctt atcaggccag
    33121 tgatgcgtac atgtttcgag tgttttactc atcagctgct gccccacttt ttcaccacgc
    33181 aacgcttcac tgacaatcac ccgacctata acgacaggct caagatcatc atcacttttc
    33241 agaatcctcg catacgccac cagttcatca tttttccaac cgagaatatg gcgattatcc
    33301 cccgtcaaat catcaccatc gatatcctga taagggcaat tctgctcgac aacaaacacc
    33361 gcgcaacgca gttgtaataa ggcataaagc tgggaaaccg ataattcaga gtgatgcaga
    33421 tcttgccatt caatcataat gctctccttt tagatgtatg ccttatacta gccggaatat
    33481 tttttgaaac gtgatgaact catggaatta atttttttag gtacttcagc cggtgtgcca
    33541 acccgcacgc gcaatgtcac ggcaatattg cttaacctgc aacatccgac ccagagcgga
    33601 ctttggttgt ttgactgcgg tgaaggcacc cagcatcagc tactgcatac cgcctttaac
    33661 cctggaaaac tggacaagat ttttatcagt caccttcatg gcgatcatct ttttggttta
    33721 cccggcttgc tgtgcagtcg ttctatgtca ggcattatcc aacccttaac gatttatggt
    33781 ccgcaaggta tccgtgaatt tgtggaaacc gcgcagcgga ttagcggctc atggaccgat
    33841 tatccgctgg aaattgtcga aattggcgct ggcgaaattc tcgatgatgg cctgcgcaaa
    33901 gtaaccgctt atccgctgga acacccactg gaatgttatg gctatcgtat tgaagaacat
    33961 gataaaccgg gtgcattaaa tgcccaggca ttaaaagctg ctggcgtgcc gcctggccca
    34021 ctgtttcagg aattaaaagc gggcaaaaca atcacgctgg aagatggaag gcagattaac
    34081 ggcgcagatt acttagctgc tccagtgcca ggtaaagcgc tcgctatttt cggcgatacc
    34141 ggcccctgcg atgccgcact tgacctggct aaaggtgtcg atgtcatggt gcacgaagcg
    34201 acgctggata taaccatgga agccaaagcc aatagtcgcg gccatagctc tacacgccag
    34261 gctgcgacac tagcccgtga ggctggagtc ggcaagctaa tcattaccca cgtcagctcg
    34321 cgctatgatg acaaaggttg tcaacacctg ttacgtgaat gcaggtcaat tttcccggcg
    34381 actgaactgg cgaatgattt caccgtgttt aacgtttaac tctgcttatt attaaaaaca
    34441 gggtgaaact tgccctgtta tagcaacccg cgctacatat atccacccac acaggcatat
    34501 tgagcgattt ttgatattta ttcatctcct tacgcactat attcagagca ataagttgcc
    34561 tcttttcatc ttgattcagc acaacctttg caactaaaag gagcccatga tggttacagt
    34621 tgtcagcaat tattgtcaat tatctcaaac ccaactcagt cagacatttg cagaaaaatt
    34681 tactgtgacc gaggaattac tgcagtcttt aaaaaaaaca gcgttatccg gagatgaaga
    34741 aagtattgag ttactgcata atattgcgtt aggttatgat gaatttggaa aaaaagctga
    34801 agatattctt taccatattg ttagaaaccc aacaaatgag accctatcga ttatccgact
    34861 tattaaaaat gcctgtttaa aattatataa tttggcacat accgcaacca actcccacct
    34921 taaaccaact ggtccggata attcagatgt tctcctgttc aaaaagctat tctccccttc
    34981 gaaattaatg acaattatcg gtaatgaaat tcctcttata tctgaaaaac agtcgctttc
    35041 aaaggtgctt ttaaatgatg agaataatga actgagtgat ggtacaaact tctgggataa
    35101 aaatcgtcaa ttaaccacag atgaaatagc ttgctatctt cagaagatcg ccgctaatgc
    35161 aaaaaatact caagtcaatt atcctactgg tctctacgtc ccctactcca ccagaactca
    35221 cctggaagac gctctcaatg aaaatattaa gagcgatcca tcatggccga atgaagtcca
    35281 gttattcccc ataaatactg gcggacactg gatattagtt tcgctacaga aaatagtaaa
    35341 taaaaaaaat aataaactac aaataaaatg cgtcatattc aactcattgc gtgcactagg
    35401 ccatgataaa gaaaattcac ttaagcgtgt cattaatagt tttaattctg aactcatggg
    35461 aaaaatgtcg aataataata taaaagttca tttaaatgaa ccagagataa tatttttaca
    35521 tgccgatctt cagcaatact taagccaaag ttgcggtgcg tttgtgtgca tggcagccca
    35581 ggaagtgatt gaacaaaggg aaagcaattc tgacagcgcc ccctatacgt tattaaaaaa
    35641 ctatgctgac agatttaaaa aatattcagc agaagagcag tacgaaattg attttcaaca
    35701 tcgactggta aacagaaatt gttatttaga taaatatggc gatgcacgaa tcaatgcttc
    35761 ttatacacaa ttagaaataa aacactcaca acccaaaaat agagcatccg gcaaaagagt
    35821 gagttaacat cattgttaat caattcgacc agtcagtaaa tatgctgact ggtcgaacat
    35881 aatttgctaa aaagtcatca ctgactgatg tcagtcccac catctgccag ttcaatctac
    35941 gggtatttcc agcgtatcgc ggcacgcgat tgctggtctg ttgatgtagt ggattgtagt
    36001 tgctgacttt tcttaccctc agatttctgc cccacttgtc aattcaatct gccaataaat
    36061 aacataatta tatttttatt cctcacagaa attccttttt aaaaatagcg aacccactgt
    36121 aaaaaacact ttttgtatac ctctaccaag cgtaataaaa taaacgctca taattcatta
    36181 taaataataa tgaaataacg ccttttattg ataatttaca tatgaaaaaa attttccttt
    36241 tgttatatta tttctgtttt cactccctct gattattttt accaaccctg ggtcactgca
    36301 ttatcaccaa cgccccgaca cgtaagtcct gagcaattag aaaaaacagt tcgttatctt
    36361 acacaaactg tgcatccacg taatgccggc aatattgata atctgaatag gtccgcagag
    36421 tatataaaag agttctttgt cagtagcggt gccagagtta ccgcgcaggg cgtccccgat
    36481 tggctattac gactctgcgc ctggaagcca gaattaccct tacccggcaa tgtcctggct
    36541 ttatcccgat cagggagact ttattgctgt ggtcggcaga atgcaggata tcaacgccgt
    36601 tcgtcaggta aaagcggcat tgttgtcatc ccgggattta tctgtttatt ctatgaatgc
    36661 cccagggttt attcccggta ttgatttttc tgatcacctt aattattggc aacacgatat
    36721 tcccgccgta atgattactg acaccgcttt ttatcgtaat aaacaatacc acttgcccgg
    36781 tgataccgca gacagattga attatcagaa aatggctcag gtggtagatg gtgttataac
    36841 cttgttatac aacagtaaat aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg
    36901 tacattttat tttccctcgt ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa
    36961 ataaatggga atgattggct attttgcaga aattgactca gaaaagatta accaactgct
    37021 tgagtctact gaaaaacctt tgatggataa tatacatgac acgctttcag gattacgccg
    37081 actggatatt gataaacgct gggatttttt acactttggg ctgacaggca cctctgcttt
    37141 tgatcccgcg aagaatgatc cgctgagtcg tgccgtgctt gatgaacaca gcctcgaaga
    37201 tggcatagat ggcttcctgg gattgacctg gaatcaggaa ttagccgcga caatcgaccg
    37261 actggaatca ctggatcgaa gtgagctgcg taaacagttt tccattaagc gactcaatga
    37321 aatggagatt tatccgggag tgaccttttc agaagagtta gaggggcaac tctttgcctc
    37381 gatcatgctt gatatggaaa agctgattag cgcgtatcga cgtatgctgc gacaagggaa
    37441 tcatgcgctg acggttattg ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac
    37501 atgtcggcgg ctttctgact tacaaactaa cagtttacat cagcggcatt gctaaccgca
    37561 caatactaat cagcggttgt ggccatacac ccagcaccag taccaacagt gcagaaatca
    37621 gcaccacgat accgcccgcg ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt
    37681 ccggtgcgtg caggtacagg ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg
    37741 aaccgacaac cacggcaccc accagccacc acaagtgtgc ctggacaccg accgccagca
    37801 cgtagaactt accgataaag cccagcgtca tcgggatacc ggccagagac agcatcatca
    37861 ccgtcatcac tgccgcgagg atcggacgat gccagaacag accgcggtaa gagaacaggg
    37921 aatcagcatc cgggccacga tacgggctgg acatcaggct gaccacgccg aacgcgccga
    37981 ggctgctgaa cagataaccg gccaggtaaa cccctaccgc ttccatcgac atctcgccgg
    38041 tttgcagcgc aatcagcgcc accagcagat agccgaggtg agagatagat gagtaaccga
    38101 gcagacgttt gatgttggtc tggctcaacg ccatcaggtt accgaagatg atggaggcaa
    38161 aggcgataat cgccagtact acgcgaatcg cttcgctgtc acccaccggt gcgtagagga
    38221 acagacgcat caccacaccg aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa
    38281 ccggcgcagg cgcgccctgg tatacgtctg gcgtccacag gtggaacggc accagagaga
    38341 gtttgaagcc gaggccaaca atcatcatgc cgaaacctgc cagcaacagc ggctcgttga
    38401 gcataccgtc gccgaggttt ttacccagcg cgacaaacga caggtcgcca gactgcgcat
    38461 acaccagcgc cataccgaac agcaggaaag aagacgctgc ggcagaaagg atggtgtatt
    38521 tgatactggc ttccagcgaa cgtttctggc ggaaagcgta accgaccagg ccaaacagcg
    38581 gcaaagagat cagttcaata ccgaggaaca gagacgccag atggtttgca ttcgccagca
    38641 ggatcccgcc cagcgcggca attaacacca acaggtagaa ctcatccttg ttgtcgttat
    38701 agccttcaag ccacgggtag tcgaaagtac aggtggcgag gctcgccaac aataccagcc
    38761 cggtgtaaag catggcgaaa ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg
    38821 cctggccaac aaaccagagc gaaaccagcg ccgcgttaag cccaataacc gagagcgtag
    38881 cgttgaggaa atgattgcgt cgccacgcaa tggagagcat cacaaccacc accgtcaagc
    38941 cgacgatcag caacggtagc agtgcgatca ggttttgtgg agttattgtc atggcgattt
    39001 acggccttgt agtagtaacg gaattaacaa accactgctg gatattgcca atcgcggagt
    39061 gcgaggtatc cagaatcggc tgcggataga agcccagcag taccagcagc accaccagca
    39121 acaggatcat aaacagctca cgcagcgaca tccctggcag ttcctggctg gcaatctggc
    39181 ttttcgcttt accgaagtaa gcgcgatgca acatcgccag cgaataaaca gatgcaaaga
    39241 ccagcccaaa ggtagagata acggtaatca ccgggacaac ctggaagctg ccgaacagaa
    39301 tcataaattc gccgacgaag ttaccggtgc caggcatccc aagcgttgcc accgcaaaga
    39361 acagcgacag tgctggcagc catttcatct tgctccacag accgcccatc atgcgcatgt
    39421 cgcgggtatg gatacgttca taaagctgac cgcagagaat aaacagaccc gccgccgaca
    39481 agccgtgcgc aatcatctgg attaccgcgc cctggtaggc caactggctg ccggtgtaga
    39541 ttgcaatcag cacgaagccc atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat
    39601 cggtctgagc gaaggccatc cacgcaccgt agaagatgcc gataacaccc agccacatag
    39661 cgattggcgc gaactctgcc gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac
    39721 cgtaagcggc ggttttcagc aagatccccg cgaggtccac ggaaccggcg gtcggagcct
    39781 gggagtgcgc atccggcagc cagccatgca gcggaaccac cggcattttg actgcgaagg
    39841 cgatgaagaa gcccagcatc aacagatatt ccacaccact ggacattggc gtattcagca
    39901 gctcttcata gttgaaggtc cagacgccgg tcgcattgta gtgaacaaaa accagcgcca
    39961 ggatggcgat caacatcacc agaccactcg cctgggtgta aatgaagaac ttggttgcca
    40021 ccgtgatacg cgttttaccg tcagaggctt tatgccccca cagtgcgatc aggaagtaca
    40081 tcggcaccag catcatttcc cagaagaaga agaacaggaa catgtcgatg gcaaggaaca
    40141 cgccgataac gccgcccagg atccacatca ggttgaggtg gaagaagccc tgatattttt
    40201 cgatctcttt ccacgaacat agtaccgcca gcacaccgag cagaccggtc agcacgacca
    40261 tcagcagcga cagaccgtca atggcgagat gaatagagat accaaaacgc gggatccacg
    40321 gcatgtcgaa ttcagactgc cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc
    40381 cctgcaacca cagttgcagc gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc
    40441 agcgtggcac cttgacgcca aagcgttcgg tctgccagca caggaagccg ccaataaagg
    40501 gaattaatat tagccagggt agtaacatgg cgatctttat tccttgtaaa agtcccgtca
    40561 ggaccggatt ttcaacgaat tctcacgaca gaaaattctc ttcggactgg gggtagtgtc
    40621 ggatgcgccg cgaaggcagc ttatccgacc tacgttctta tatgcccagg ggcaatccca
    40681 caatccttaa ctcaacgcag taccatcaac agtgccagca ccacgaccgc accgatgctc
    40741 atggatgcca cataccagcg cagataaccg ttctcgctta acagcagacc tttacctgca
    40801 aagcgggaaa gaaccgccgg gatgttcatc attgagttca gcggatcgcg tttcagcaac
    40861 caggcaatac ccaggaacgg cttgacgaac actttgtcgt acagccagtc aaagccccag
    40921 gcgttgtacc accaggtact cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc
    40981 agagtacgtt tacccagcca cagccaggct gccagcagaa tgccgaccac cgcgaccacg
    41041 ccagaggtaa tttccagggt caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc
    41101 acgccctgca gcggcggtac aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca
    41161 atcagcggca ggctgtgagt tacccctttc acggcgtgag cgtgaatttg ttcttttccg
    41221 tggaagacga tgaaaatcat acggaaggtg tagagcgagg tcataaacgc accgaccaga
    41281 cctgccacca tcagattgat atgaccattc gccatcgcac ccgcgaggat ctcatcctta
    41341 ctgaagaagc ccgcagtgac cagcggcagt gccgacagtg ctgcgccgcc caccaggaag
    41401 cagagataaa ccagcggaat agatttacgc agaccgccca tcttgaagat gttctgttcg
    41461 tgatggcagg ccagaatgac ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg
    41521 tgagtcatca agtggaaaat cgccgcatcc catgcctgca cgccaagcgc gaggaacatg
    41581 tagccaatct ggctcatggt agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc
    41641 gcggcaaaac cggccagcag cagcgtaacc gccccgacaa tacccaccag atgcagaact
    41701 tccggcgtca tcaggaacag gccgtgggta cgggcgatca ggtagacacc cgcggttacc
    41761 atcgttgcgg cgtggatcag cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc
    41821 catgtctgca acggcaactg cgcagattta ccgaccgcac cgcccagcag catcagcgtc
    41881 gcccacatca gcatgttatt gccgtcagca aagtgcgctg gtgccagttc caccatttcg
    41941 cggaagttca gggtgcccag ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg
    42001 tcacccacac gggtcacgac gaacgctttc attgctgctg cgccattctt cggatcggtg
    42061 taatagaaac cgatcagcag ataggagcac aggcccacgc cttcccagcc gaggtacatc
    42121 agcagcaggt tatcggcgag aaccagaact accatgctgg cgatgaacag gttggtgtag
    42181 gcgaagaagc gagagtagcc ctcttcaccg cgcatgtacc aggaggcgta catgtgaata
    42241 aggaaaccca caccagtgac caccgagagc atggtcagcg acaggccgtc cagcaccagg
    42301 ttaaaaccga tgttaaagtc gcctaccgac atccacgtcc acagcggctg gctgtatgcc
    42361 tgctcgccgt tagcgaagaa atcaacgccg ataaaggcgg ttacctgcgc cgccaggccc
    42421 acagagccaa cgccgacgat cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc
    42481 agcaggacga agccaatcaa tggcaaaata atggttaagg caagcatgtt catccgcgca
    42541 tctcacttac tgaatcgatg ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg
    42601 caaggccgat actcgcttct gccgccgcga ggctgatggc gagaatgtac atcacctgac
    42661 cgtcggtctg accccagtag cttccggcga ccacaaaggc cagcgcggag gcgttaatca
    42721 tgatttccag accaatcagc ataaacagca gattgcgacg gataaccaga ccggttaagc
    42781 caagaacgaa taagattgcc gcgaggatca gtccatgttg taaggggatc atgcgtgctc
    42841 ctccgttttt cttttcgcgc tgtcgtcttt acgattgctc agcacttcac ccgcacgctc
    42901 ttcacgaccg acgtggaagg ccacaaccag acccgcgagc agcagcatag aagccagttc
    42961 caccgccagt acgtaaggcc cgaacagcgt aatacccact gctttagcac tgattggcgt
    43021 accgtcgata ccctgatcgt taacaccgag gatggcgtaa acaatcacca ccagcatgat
    43081 ggccgacaaa attgccgggc caatccacac ctgcggtttc agccactggc gttcctgttc
    43141 gatttctgaa ccgcccaggt tgagcatcat caccacgaac acgaacagca ccataatggc
    43201 acccgcgtag acgataattt ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac
    43261 cccggaaatc gccagcagcg aaataatcag gtacagcagt gcgtgtaccg gattggtatg
    43321 ggtgatcact cgcaaggtcg caagtatggc tatcaggcca cagatataaa aagcgaactc
    43381 cattgcccct ctccttacgg taacaggctc ttgacgtcga taggcttggc ttcgttctct
    43441 gcttcgccct tatctttgcc gtcgattgcc atacctgcca tccggtagaa gttatattcc
    43501 gggtatttgc ccggaccgga gatcagcaga tcctctttct cgtaaaccag atcctggcgc
    43561 ttgtattccc ccatttcgaa atccggggtt aactgaatcg ccgtggtcgg acaggcttct
    43621 tcgcacagac cacagaaaat gcagcgtgag aagttaatgc ggaaaaattc cggataccag
    43681 cgaccgtctt tggtttctgc tttttgcagc gagatacagc cgaccgggca ggctaccgca
    43741 cagaggttac aggctacgca acgctcttcg ccgtccgggt cgcgggtcag aacgatacga
    43801 ccacgataac ggggcggcag atagaccggc tcttccgggt acattcgcgt ttcgcgtttg
    43861 gcgaacgcgt gcaggccgat catccagata ctacgaacct gggtgccgaa acctactaac
    43921 aattctttta aggtcatggt cttattgccc cttattgcgc ctgccagaga atgacagccg
    43981 ccgttaccag caagttgatc agcgtcagcg gcaggcagat tttccagccg aaggacatta
    44041 cctggtcata acgcggacgc ggtaacgacg cacgaatcaa aatgaacatc atcataaaga
    44101 acgcggtttt cagcgcgaac cagatgaatg gcggtaacaa cgggccttgc cagccaccga
    44161 agaacagcgt caccatcaat gcagagatgg tcacaatccc gatgtactca cccacgaaga
    44221 acagaccgaa cttcataccg gaatattcaa tgtggtaacc atccgccagt tcctgctcgg
    44281 cttccggctg gtcaaacggg tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa
    44341 taaaaccaaa gaattgcggg atgacgttcc acacatgcgc ctggctgttg acgatgtcgg
    44401 tcatgttgaa tgaaccggcc tgcgccacca cgcccatcag ggaaagcccg aggaacactt
    44461 cgtagctcag ggtctgcgca gaagcacgca tcgcacccag cagcgagtat ttgttgttac
    44521 ttgaccagcc cgcaaacagc accgcgtaaa ccgccagacc tgccatcatc aggaagaaca
    44581 aaatcccgat gttcaggtcg gcaaccaccc aacccggact gactggcaca attgcaaagg
    44641 ccagcagcag cgaggtaaag gcaatcatcg gtgccagggt aaagatgacg cgatccgaga
    44701 atttcgggat ccagtcttct ttaaagaaca ttttgatcat gtccgcaacc agctggagcg
    44761 aaccgcccca gccaacacgg ttaggtccat aacggttctg gaacagaccc agcaggcgac
    44821 gttcgccaaa gctcatgaat gccccacagg tgacaaccac cagcaggatc cccaccgctt
    44881 tgaggatggt cagcaggatc tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc
    44941 cttgagatcc tcaagatgcg cgccagccag caccggagca atgccggaca tacccatcgg
    45001 caagcccacc tgccctgccg tcagtccttc ggcgatttca accggcagcg tgaccgtatt
    45061 gccatcgtaa ctaaaggaga cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc
    45121 tgggttgagt ttgatgtacg gctgcggcat acggctctgg aagaccggag cacgctgtga
    45181 caattcatcg ctgccaaaca ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg
    45241 ctggaagcgt gccggtacgc tggtgaagta atccagaccg ttttcgctgg tttcaaacag
    45301 acgcacgccc ggatcgccaa agcgcagttt gccgcccact tcgtcctgga atttgttcca
    45361 cgcctgcggg gagttccagc ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt
    45421 cggctggttg ttaccttcca tcgagaaggt gaacatggtg tcaatatcct gcggctgacg
    45481 cggctcatga acgctgatat tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc
    45541 acgggccagt ttctgcccac gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc
    45601 cgccagttcc gggattttcg ccacaacagc gtcaattaca tggtcgagct gcgtccagtc
    45661 cacttcacgg ctcagcaagg tgctgtgcag cgagtgcagc cagcgccagc tttccagcat
    45721 gacagttttg ctgtcgtaat aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc
    45781 ttcattatta attaccgtac cgtcgctttc agcaaagctg gcagcggaaa gcaccagatg
    45841 ggcgttttcc atgatcgctg tgcgttgatg atcaaccacc atcaccagcg gcgctttagc
    45901 cagcgcagca ttcacgcggg tagcagaagc gtaacgatgc agatcgtttt ccaacaccac
    45961 caccgcgtcg gcgcgtccgg tttccagttc ggttaacgct tcttcaagcg aaccgccacc
    46021 cataatgccc agccccatgc tgttgacgga acgggcaatc atggtgatac caacgtcagc
    46081 gccgcgacct ttcagggctt tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc
    46141 ggcgttcgtc ccggagataa tcaacggttt cttcgcacct gccagtgcct gcacgatgac
    46201 gtcgattttg ttttgcagct caggttcgat accgtcaacc gctggtgcgg agttatccag
    46261 cgcatgggcg atggcaaaac ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca
    46321 cgccgcgata tcatccagac gagtgtcatc aacgttggta acaaacagcg gatgcttcgc
    46381 acgttgaccg atgttgagga ttgccgcaat ctgccagtca gccactttct gtgctgcagc
    46441 catttcgcgc gctttacctt tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt
    46501 ctgggtaacg tcttcgccca gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc
    46561 cggagtataa atgccgcctt cacgcagcac tttcagcgcc agttgcagac gttcctgctc
    46621 accgtgagcg ataccggtgt agaagttttc ttcgcccacc agttcacgca gcgcaaagtt
    46681 gctttccacg ctggcacgcg gagaaccaat accgatcact ttcttcgact gacgcagaat
    46741 atctgccgcg ccctgcattg cctgttcggc gttgagggta atgaaatcat cgccacgacg
    46801 ctgtactggc tgacgcggac gatccttcag gttgacgtaa ccgtaaccga aacgaccacg
    46861 gtcgcagagg aagtagtggt ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc
    46921 gccgtaacgt tcaccggggc tgatgttaca gccgatggaa cattgctggc agatgctcgg
    46981 cgcaaactgc atatcccatt tacggttgta acgctcggag tgcgttttgt cggtaaatac
    47041 gccggtcggg caaatttcga ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc
    47101 cgggcgaccg aagtagacgt tgtcgtgcgc gccataaacg cccagatctg taccgtcagc
    47161 gtaatctttg tagtaacgca cacagcggta gcaggcgatg cagcggttca tttcgtgaga
    47221 gatgaatggc cccaaatcct gattacggtg ggtacgtttg gtgaaacggt agcgacggaa
    47281 gctgtgtccg gtcatcacgg tcatatcctg aagatggcag ttaccgcctt cttcacatac
    47341 cggacagtcg tgcgggtggt tggtcattaa ccactcgacc acactttcac ggaactgttt
    47401 cgcttcttcg tcgtcaatgg aaataaaggt gccatcggaa gccggtgtca tacaggacat
    47461 caccaggcga ccacgcgtgt cttccgcgtt ttggtattgc ttcaccgcac actggcggca
    47521 agcaccgacg cttcccagcg ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga
    47581 cagacaagct tccagcaggt tgtccgctcc gttgacctcg tattctttgc cgtctacatg
    47641 aattgtagcc attagcatgc ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat
    47701 tcggttacca gcgctctttc agcaggttcg gctgaatccc attaatcaaa tgggtattgc
    47761 tgaacggctg tttgattccc gcctcaaatt cttcgcggaa atatttgatg gcgctctgta
    47821 acggctccac tgcaccaggt gcgtgggcac agaaagtttt acccgggcct aagaatcgac
    47881 acagttgctc aagtgtttcg atatcgcccg gctggccttc gccacgctcc agcgcacgca
    47941 gaattttcac gctccacggc agaccgtcgc ggcacggcgt acaccagccg caggactcac
    48001 gggcgaaaaa ctcttccagg ttacgcacca gcgacaccat gttgatctca tggtcaaccg
    48061 ccatcgccag cgccgtaccc agacggctgc ccgctttacc gatactttcg aattccatcg
    48121 gcagatcaag gtgcgcttcg gtcaggaagt cagtccccgc gccgcctggc tgccaggctt
    48181 taaatttcag accatcacgc ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg
    48241 tgccgaacgg cagttcccac agtcccggat ttttcacccg accggagaag cccatcagct
    48301 tggtgccagc atctttactt ttcgagatgt tctgatacca ctccacgccg ttagcgagga
    48361 tcgccggaac gttacacagg gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc
    48421 cggaggttgc cgggaagggg ggtttcgagc gtgggttagc acgacgccct tccagggagt
    48481 tgattaacgc tgtttcttcc ccgcagatgt agcgccctgc cccggtatgg acgaacagtt
    48541 cgaagtcgaa acctgttccc ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg
    48601 caatagcgcg gcgcagattt actgccgctt cgatatattc gccacgcagg aagatgtagc
    48661 cacggtaagc tttcagcgca aacgcggaga tgagcatacc ttccaccagc aggtgcggca
    48721 gttgctccat caacaggcgg tctttatagg tgcccggctc catttcatcg gcgttacaca
    48781 gcaggtaacg gatgttcatg gattcgtctt tcggcatcag gctccacttc aggccagtcg
    48841 aaaagcccgc gccgccgcgc cctttcagac cagcgtcttt tacctgatta acgatttcgt
    48901 ccggagacag cccggtcagc gccttacgcg ccccttcgta accgtttttg ctgcggtatt
    48961 cgtccagcca cactggctgt ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg
    49021 gagtacggat aatgtttttc atttataccg ctccagcagt tcagggatcg cttccggggt
    49081 cagatgcgcg tgagtgtcct catcgatcat catgtttggc cctttatcac agttccccag
    49141 gcagcaagtt ggcagcagcg taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt
    49201 cagctttttc tcgagcgccg cctgaatacc ctgatacccg ttgatatgac agaccacgct
    49261 gtcgcaataa cggatcacat ggcgaccaac cggctggcgg aagatctgac tgtagaacgt
    49321 tgccacacct tcgacgtcgc ttgccggaat acccagcaca tcggcgatcg cgtggatcgc
    49381 accatccggc acccagccac gctgcttctg aacgattttc agcgcttcaa tggacgccgc
    49441 acgcgggtct tcgtagtggt gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag
    49501 ctcaaaagcc tcggtttgtg gttgttgatt ctcgtgcata attagcggtc cacatctgac
    49561 ataacaaaat cgatactgcc cagataaaca atcaggtcag acaccaggct gccgcggatc
    49621 gccgccggaa tttgctgcaa atgcgcataa ctcggcgtgc ggatacgagt gcgataactc
    49681 atggtgctac cgtcgctggt caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc
    49741 tggaaagatt cattggcagg catcaccggc ccccacgaca cttgcaggaa gtgggtgatc
    49801 agggtttcga tatgttgcag cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc
    49861 gctttgaacg ggccttccgg catgttgttg aggcactgct caagaatgcg cagactctgg
    49921 cgcagctctt ccactttcag cattacgcgg gtgtagcagt cagaaacacc accaccaacc
    49981 gggatttcaa agtcgaagtt ttcatagcca gaataaggac gcgccttacg cacgtcgaag
    50041 tcgatcccgg tagcacgcag gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg
    50101 ccataggcgg caacgccctg ggtacgacct ttcagaatgg tattttgcag cgccgctttc
    50161 tcgtaagacg ccagacgttt cggcatccag tcgaggaact cacgcagcag acgatcccag
    50221 ccgcgcggca ggtcgtgcgc tacgccgcca atacggaacc acgccgggtg catacggaaa
    50281 cccgtgatcg cttccaccag atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg
    50341 ggcgtcattg cgccgacgtc ctgaataaag gtcgagatgt acagcaggtg gctgttgata
    50401 cggaacagtt cggagagcat aacgcgaatg acgttaacgc gatccggcac ggtgatcccg
    50461 gccagtttct ctaccgccag cacgtaaggc atttcgttaa cgcagccgcc gaggtattca
    50521 atacggtcgg tatacggaat gtagctgtgc caggactggc gctcgcccat tttctccgca
    50581 ccacggtggt ggtagccgat gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt
    50641 aaaacgatac ggaaagcccc gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg
    50701 aagtcctcgt tttcggtgcc gcgcttcatc ccccactctt ccggtttgaa ggtcagggct
    50761 tccatctcca gatcctgttt ggctttggtc agctcaaacg gcgagaattc ggtagcgcgc
    50821 gccgggtaat ctttacgcag cgggtgacct ttccaggttt gcggcatcat gatgcggcgc
    50881 aggttcgggt gaccgtcgaa agtaatgcca aacagatccc aggtttcacg ctcataccag
    50941 ttagcgttcg ggaacagttt ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc
    51001 accttcagca tgatgtcgcg gttacgatcg atagaaatca gatggtagaa aacggaaaaa
    51061 tccgcggcag gtaacccttc gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca
    51121 aacagcatga cgtaaggttt cggcagtttc tttaagaaat cgccaacttc cagtaattgt
    51181 tcacgcttga tccacacaac gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc
    51241 ggcccaaaac ggttgcgcag ttcgccaatt accggatcat caagatgatc gcgggtctgc
    51301 caggcgggtt cttgcgcggt taagtcggtc atattgttca ccattgcaaa tggtccgtgg
    51361 tgactgtcgg cgcagtaagc ttcgcgaaat agatttagga ataagcgaag aaaaatccct
    51421 ttgccgacag gcgcaaatta aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt
    51481 tcaccgcgct tgcgttcgcg ctctgattgc atattggcgc gataaacgcc ctgatcgcca
    51541 accacccagg agagcggacg acgttctttg ccgatagatt cctgcaacag catcagtgcc
    51601 tgcatgtacg cttcaggacg cggcgggcag cccgggatat acacatcaac cgggatgaat
    51661 ttatcgacgc cctgcacaac ggaataaata tcgtacatac caccagagtt ggcacaggcc
    51721 cccattgaga taacccattt tggttccagc atctggtcat acagacgctg aataaccggt
    51781 gccattttgg taaagcaggt tcctgcaacc accatcaggt cagcctgacg cggcgaagca
    51841 cgcaatactt ctgcgccaaa acgcgccacg tcatgcaccg cggtaaacga agtcaccatc
    51901 tcaacgtaac agcaggaaag accgaagtta tacggccaaa ttgagttttt acgaccccag
    51961 ttaaccatgt cattgagctt gcccataaac acgtttttgt taacttcttg ctccagaggg
    52021 tcggttacga tctcctgctt ttgcaggggg taacggtcgt tctcaccgtt gggatctatg
    52081 cgggtgagcg tataatccat cttaatgcct cgcggttagc gttgacgatt agcgatactg
    52141 ttcgtttccg ggttcataca ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca
    52201 atacgcacca gataaaccag acctgccagt aacacaaaaa taaaaattgc agcttccaca
    52261 aagcctaccc agccgctttc gcggatagag gttgaccatg cgaacagata cagcgcttca
    52321 acgtcgaaga taacgaagaa catggccacc agataaaact tggcggacag gcgtaagcgg
    52381 gcggagccga ccgaatcgat accggattca aacggcacgt ttttcgacct cgcgcgtgcg
    52441 cgaccgccta aaaaccaacc gcctaccagc atcaggcaac acaggccaat ggcaacgata
    52501 agaaagatag cgaatgccca gtgatgagcg atgacttcag tggatgttga catactcatt
    52561 gcttactcat caaaagtagc gccagattca ctgctcttca cggcagatgg acgccacatc
    52621 gattcatggg gaggaataaa aaaaacctta caatcactgt agaaattctt ttatacagct
    52681 aattgatgtg gtcttttact cctttctata accttttgtc aactttaaca aaagtttctt
    52741 cacattagtt tacataatat caacaccatt agcatttaat gccctttcac cccagatcct
    52801 tgacgactcc aggataatta gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa
    52861 atacgggtct attttgacag gaatttgtgt cgattcctcc ccccaaaaga gagtattttc
    52921 ttgatctgtg acacgctttt gttattccat aacaaaaacg cagcaacaaa tttacgtatt
    52981 ttttaacatc attgtagcag gtgatttttt tcaggcgatt atttgtgcgt tcgggacgtg
    53041 aatctctggt gattgaaaaa taaacagttt tgtacgttct gcactatgcg aggaaggatt
    53101 tttactaaaa aaaagccgct ggggtttaaa acacccccag cggctcgttt tttacactat
    53161 tgtctcagga attatctatc gtccgtcgat tactcgatat ccctttcaat caacaatgaa
    53221 tcatcccctt ccggagcaga cattgggctg tattgccacg gattatggta gctctccatc
    53281 gcctgataga tcacctgggc cagctcatta ttactggacg ggtcatagca cagcaaatat
    53341 tcggtatcag gtagcggcgg taagccatct actccactca gcacacgcag gtccgggctc
    53401 atcatctcaa ccggccttgc cgtcacgcca agaccggctt tcactgccgc acgaacggcc
    53461 ggaagcgtcg aggcgacata agccagtcgc catggaatat ctgctttatt aagcgtcgcc
    53521 agcaccatat cgcgaaacgg acttggatca tccagcaata caagagggat cggctcgcct
    53581 ttttgcaata cgtattccgc tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat
    53641 gccttaaacg ctgaaggacg atgggtggtt accatcaaat ccacttcctg ggattccagc
    53701 atttctgcca tataggcatt acgtttaacg cggacatcca gcgctaattt cggataaacc
    53761 gaactcacgc gatttaacag gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg
    53821 atagttaaca cgccctgaag attactgaac attaatgatg agcaggcctc atcattaaaa
    53881 cgcaggattt tcctggcgta accaagaagt tgaatgccat gttcagttaa cagtttgttg
    53941 cgaccgtgac gagcgaacag ttctttccca acgagttgtt ccagacgctg catttgctga
    54001 cttacggcgg actgagtacg acacacagcg gcagctgcgg cagcaaaagt gttcagatcg
    54061 gcaacagcaa caaatgttct cagcagatcg aggtcgaggt taattatcgg acgatttgca
    54121 cttatcatat attatcactt actggcggct catactgagc tggttaatgc tgtgcacaca
    54181 caaacaagca attccatttg taatgtgcct ccctggcagt ttcatccgga acccggacga
    54241 aagtaaaaat gcatatgagt tgcactaaaa aagcgactca cattgttccg ttataatgcc
    54301 tgaagtagat cacagaatat atcttcaggg atcgcatatc tattaagtta ctcactcttt
    54361 tctatttatg acatgcgcgt gtttgtataa atgtaaatgt gagtccttgt tccactctcg
    54421 tgcagcatcg ctggtcatac gcgaacacgt acaaacagca atggtgaagc ggcatcaaga
    54481 gcagggatcc gtcatttatg cgagcatttt accccaaaac cttttattta taagggtcat
    54541 tgcgaattat ctgatgcaaa gttatgttat gttatgttat gttatgttat gttatgttat
    54601 gttatgttat gttatgttag gcaaagtaat cttctattat taataagcac atcaaaacct
    54661 ttttgaatat taaataataa ttaattagca tcatcctcat tcattaattc cgcttaacaa
    54721 tagtttcaca ccttatcccc attgcgacac ctcggaaatc atcacgctgt taaactctcc
    54781 gtaatgcgga aatatcatca tcgcgtttca ttagtgaatt cttaaatgag gcattttcac
    54841 acaattatct tacagataaa aaaaccagac ttgcaattaa gaattagaac aattcactat
    54901 ataacattac atgtaaagca tatacacctc attattttgt cattattaag ttattaacag
    54961 cacaatcgag ccttcccctc tggcaaaatc ttattctgca gaccttcaaa acaccgtcct
    55021 gggggagtac attgttctaa gctgacttcc acggcaggga gtggcgataa cagcaaaaaa
    55081 ggtcaagatt catgtccccc attgaaaaat ccagcaaatt agagaatgtc tgttatgaca
    55141 tccgtggtcc ggtgctgaaa gaagcaaaac gcctggaaga agaaggtaac aaggtactga
    55201 aactgaacat cggcaaccca gccccattcg gttttgacgc gccagatgaa atcctcgttg
    55261 acgtgatacg caacctgcct accgctcaag ggtattgcga ttccaaaggg ctttactccg
    55321 cgcgtaaagc catcatgcag cactaccagg ctcgtggcat gcgtgatgtt accgtggaag
    55381 atatttacat cggcaatggt gtatcggagc ttatcgttca ggcaatgcag gcattgctga
    55441 acagcgggga cgaaatgttg gttcctgcac cagattaccc actctggacc gcggcggttt
    55501 cgctttccag cggtaaagcg gtgcattatc tttgcgatga atcctctgac tggttcccgg
    55561 acctcgatga tattcgcgct aaaattacgc ctcgtacgcg tgggattgtt attatcaacc
    55621 caaataaccc aaccggcgcg gtatattcca aagagctttt aatggagatt gtggagattg
    55681 cacgtcagca taatctcatt atcttcgccg atgaaattta tgacaaaatt ctctacgacg
    55741 atgctgagca tcactcaatt gcgccgctgg cacctgacct gctgaccatt acctttaacg
    55801 gactgtcgaa aacgtaccgc gttgcaggct tccgtcaggg gtggatggtg ttgaacgggc
    55861 cgaaaaaaca cgccaaaggc tacatcgaag gtctggaaat gctggcttca atgcgcctgt
    55921 gtgctaacgt tcctgcgcaa cacgccattc agaccgcgct gggtggttat cagagcatca
    55981 gtgaatttat tacccctggc ggtcgtcttt atgagcagcg taaccgcgcg tgggaactga
    56041 tcaacgatat tccgggcgtt tcctgcgtga aacctcgtgg tgcgctgtat atgttcccga
    56101 aaatcgacgc caaacgcttt aacattcatg acgatcagaa aatggtgctg gatttcctgt
    56161 tgcaggaaaa agttctgttg gtgcaaggga cggcattcaa ctggccgtgg ccggatcact
    56221 tccgcattgt cacgctaccg cgtgtcgatg atatcgagct gtctttgagc aagttcgcgc
    56281 gtttcctttc tggttatcat cagctgtaat cttaatttca ctgccggaga ttgcatccgg
    56341 cagtgttatc ccgccacaat gacctgatga tgtcatcata cgtaaggtca ctatgaaaca
    56401 gagccatttt tttgcccatc tctcccgcct gaaactcatt aaccgctggc cgctaatgcg
    56461 caacgtgcgg acggaaaatg tgtccgaaca cagtttgcag gtagcgatgg tcgcccatgc
    56521 gctggcagct atcaaaaatc ggaaatttgg cggtaatgtc aacgccgaac gtatcgcttt
    56581 actggcgatg taccacgatg cctcagaagt gctcaccggc gatctcccta ctccggtgaa
    56641 atacttcaat gcgcaaatcg ctcaggagta caaggctatt gaaaaaatcg ctcagcagaa
    56701 actggtcgat atggttccgg aagaactgcg ggatatcttt gcgccgttaa ttgacgagca
    56761 tgcatatagc gatgaagaaa aatcgctggt gaaacaggca gatgcactgt gtgcatatct
    56821 gaaatgtctg gaagaactcg cggccggaaa taatgaattc ttgctggcaa aaacgcgact
    56881 ggaagcgacg cttgaagcgc gtcgcagcca ggagatggac tacttcatgg aagtatttgt
    56941 tcccagcttc catctttcgc tcgatgagat tagccaggat tcaccgctgt aagcagccgg
    57001 agtctgcgtc gcatcaggca ataagcgccg gatgcgacat caggctcttg tcaaaacgga
    57061 aacagcatcg ggatcatcac cacacaaacc gccatcacga taatggtgaa cggtaccccc
    57121 aacttcacaa agtcactgaa gctgtaattt cccggaccta aaaccagggt gttaacaggt
    57181 gaagacaccg gtgtcataaa ggcggcggat gctgccatcg ccacgaccat cgcaaatgga
    57241 taaggcgaca ctcccatcgt tttggcagca gccagcgcaa tcggagccat caacaccgcc
    57301 gtcgcggtat tagagataaa cagcccaata accgccgaca agacaaacaa acaccccagc
    57361 atcatatgtg gcccataacc gccgccaata tccatcagcc ctttcaccgc cagcgcgaca
    57421 cctcccgttt tctgtaatgc cacagcaaac ggcatcatcc caacgatcaa aataatgctc
    57481 ggccagtgaa tggatttata ggcgctttca gcatctatac agcggaattt ccccatcagc
    57541 aggcaggcga tgatagcggc aacaggatta ggaatttcat ttgtcagcat taacgccacc
    57601 attagcacca gacagaaaat ggcatggggt gcctggctgt gcgcgggcga tgcttcactc
    57661 acctcttccg gtaagttcag cgctacgaag tcgcggccct gtttggccag cataccgatc
    57721 agtttccagt tacccacaac caggatgata tcgcccagca gcagaggctc atccgccagc
    57781 gaaccttcca gcgccacgcc attgcgcttg agccccacca cattcagtcc gtagcgggta
    57841 cgaaaaccaa tttcgcgcac cgatttacca atcagttctg actcaggaat taatgaaatc
    57901 tctgccatgc ccacatcaag ggcctggtca gaaaaatact cgccgcgcag taccatcggc
    57961 tccagcaatt gctcactaca aaattgccgg agatcgacat cagccgcaga catatcaata
    58021 agcaaaacgt cacgcgcgcg aaattcagaa accccattaa cgttcacgat aacgcgacga
    58081 aaacgccgcc agcgttcaac accgatgacg ttagcgccat aacgctcacg taatttgaga
    58141 tcatccagcc gttgacctat cattggcgat ccggggcgaa tagccagacg tcgcgcacgc
    58201 ccggtcagtc gatattcacg gataagatcg cgaaaggttc gacgcgtcca gccttcgcgc
    58261 tgcggggtct gggtatcccc tttcagcatg aaacgcatca ctaacatata caaaataccc
    58321 agcaccagca cgaccaggcc aataggtgtt acgctaaaga aactgaagcc gtgatagcct
    58381 tcacgcagca attcactgtt gactaccagg ttcggcggcg tcgccaccag cgtcatcatg
    58441 ccgctaatca gcccggcaaa acttaacggc atcatcagac gtgacggcga agtttgcata
    58501 cgcatggcaa cgcttaacac cacgggaata aagatagcga caacgctggt tgaactcata
    58561 aacgcgccaa gccccgcgac ggtcagcatc aacaaaacca acattttgat ttcactgttg
    58621 cccgccactt tgaccagcca tgttcccatt acggtggcaa caccggtacg gaccaaacca
    58681 tcgccaataa taaacaaggc ggcaatcagg acaacgttag gatcagaaaa gccggaaaat
    58741 acttcgggga ccgtcagcgt tccgcttaat gcaaacgcga caataacaaa caaagcgacc
    58801 gcatccatac gcactctgcc cgtcgcaaac aagacgatgg caacggccag taatgaaaga
    58861 acccaaatca attcaccgtt cacaacttat ccttgttaat tgagggggat gacttgattc
    58921 tgccataaaa aagccccgac gagacggggc taaatcatga tcaggtgttt cactgaataa
    58981 taacatcgcc atttggctgt ttggtcacag taatttgctc cagactgtgg aggaccaaat
    59041 cgacctcatt caggcgcggg gtatctgccg gagcgttaac cgcaatgaca tggcaacccg
    59101 ccgctaggcc agaaagcacg ccagcgggag catcttccac caccacacac tcctgcggcg
    59161 caagccccag cagctgcgca cctaacagat acgcatcagg ttctggtttt ccgcgcttca
    59221 ctcgctcagc ggttacaaac acctctggtg cgggaagccc cgctatttta tggcgcgctc
    59281 gcgctaccgg catggagcca gaagtcacaa tggcccacgg aatacctgct ttattcaaat
    59341 gattgagtaa ggcgattgcc ccagaaagcg cggtaatacc ttcggtttcc gtggcctcga
    59401 tttgctccag acgcgtaaac tcggcggcaa tatcagcctc ggatttgccc gccataaaat
    59461 ggcgcaaaga ggtgatcgcc tgtttaccgt gaatgaaagc cagcacctct tccggcgcta
    59521 acccatgacg tctggcccag ttgctccacg cccgttctac cgcaggcagg gaatccacca
    59581 gcgttccatc aagatcaaac agaaaacctt tgcaccgcac gcgggcctcc tcaggcgttg
    59641 ataatttgat taatttcgtt ggcgctcaaa tggtactgac gcgggcaggc atgccacaca
    59701 ttaagcatgc gctgatattt ttcccacatt ggcgtctggg cgttaaaacc atgagtccca
    59761 gcgtcaaaat gggtatagcg cccttccaca ttaaccataa agcggacata accgaggtaa
    59821 cgtgcttcag tggcggcgtc aaagccgagg aaggtgacac gacgttcatc gatggattgc
    59881 tggtcctgca aattagacca ggaaacatgc aacgcatgat acatctccat aatgtcgatg
    59941 atagtgcggc aggtttcttc tttcagctcg ccaaactcgc gatccaattc acgcatctgt
    60001 aatccgtaac cacgctcaat aattgtttgc aggcgacggt aacgttcagc atttgccgga
    60061 tcgagcatag tcatcatctt gtactgatta gacaaaataa gacgttgcgc gttggtcatt
    60121 tccattgttg actcctgtat cactctacta cggtgaaaaa aaagaaggct gagtatgcct
    60181 tcttttatat gcgtaatcag gggtcaatta caaatcatca aggaaagttt tatccagttg
    60241 tttgaaggcg cgcttaagcg tgtcagctaa tgcctggtaa tcaggcttgc cttcaacggg
    60301 tgccaacacc tgtccagact cctgcaattt accgcgaact tcataaaacc agttaaggat
    60361 tgcagggggt aatggcgtta cagaacgctt gcccagccac cacaatccct gcatgggtaa
    60421 acttaaggcg aacagtgcag tggcaactgc cggcccaagc agaccgccca gggcaatctg
    60481 ccagcagaga gtaaatacgg cgatcggcgg cataaaacgg atcgcataac gcgtcatctt
    60541 gataacgcga ttttcgacaa agaccggggc aaggcgtttt tccagtggcc acgtctttga
    60601 gtaatgctgt ccgcggcgaa acaagctaaa aaaattaaca gaacgattat ccggcgttga
    60661 catgcttcac ctcaacttca catataaaga ttcaaaaatt tgtgcaaatt cacaactcag
    60721 cgggacaacg ttcaaaacat tttgtcttcc atacccacta tcaggtatcc tttagcagcc
    60781 tgaaggccta aatagtacat attcattgag tcgtcaaatt catatacatt atgccattgg
    60841 ctgaaaatta cgcaaaatgg catagactca agatatttct tccatcatgc aaaaaaaatt
    60901 tgcagtgcat gatgttaatc ataaatgtcg gtgtcatcat gcgctacgct ctatggctcc
    60961 ctgacgtttt tttagccacg tatcaattat aggtacttcc atgtcgagta agttagtact
    61021 ggttctgaac tgcggtagtt cttcactgaa atttgccatc atcgatgcag taaatggtga
    61081 agagtacctt tctggtttag ccgaatgttt ccacctgccc gaagcacgta tcaaatggaa
    61141 aatggacggc aataaacagg aagcggcttt aggtgcaggc gccgctcaca gcgaagcgct
    61201 caactttatc gttaatacta ttctggcaca aaaaccagaa ctgtctgcgc agctgactgc
    61261 tatcggtcac cgtatcgtac acggcggcga aaagtatacc agctccgtag tgatcgatga
    61321 gtctgttatt cagggtatca aagatgcagc ttcttttgca ccgctgcaca acccggctca
    61381 cctgatcggt atcgaagaag ctctgaaatc tttcccacag ctgaaagaca aaaacgttgc
    61441 tgtatttgac accgcgttcc accagactat gccggaagag tcttacctct acgccctgcc
    61501 gtacaacctg tacaaagagc acggcatccg tcgttacggc gcgcacggca ccagccactt
    61561 ctatgtaacc caggaagcgg caaaaatgct gaacaaaccg gtagaagaac tgaacatcat
    61621 cacctgccac ctgggcaacg gtggttccgt ttctgctatc cgcaacggta aatgcgttga
    61681 cacctctatg ggtctgaccc cgctggaagg tctggtcatg ggtacccgtt ctggtgatat
    61741 cgatccggcg atcatcttcc acctgcacga caccctgggc atgagcgttg acgcaatcaa
    61801 caaactgctg accaaagagt ctggcctgct gggtctgacc gaagtgacca gcgactgccg
    61861 ctatgttgaa gacaactacg cgacgaaaga agacgcgaag cgcgcaatgg acgtttactg
    61921 ccaccgcctg gcgaaataca tcggtgccta cactgcgctg atggatggtc gtctggacgc
    61981 tgttgtattc actggtggta tcggtgaaaa tgccgcgatg gttcgtgaac tgtctctggg
    62041 caaactgggc gtgctgggct ttgaagttga tcatgaacgc aacctggctg cacgtttcgg
    62101 caaatctggt ttcatcaaca aagaaggtac ccgtcctgcg gtggttatcc caaccaacga
    62161 agaactggtt atcgcgcaag acgcgagccg cctgactgcc tgatttcaca ccgccagctc
    62221 agctggcggt gctgttttgt aacccgccaa atcggcggta acgaaagagg ataaaccgtg
    62281 tcccgtatta ttatgctgat ccctaccgga accagcgtcg gtctgaccag cgtcagcctt
    62341 ggcgtgatcc gtgcaatgga acgcaaaggc gttcgtctga gcgttttcaa acctatcgct
    62401 cagccgcgta ccggtggcga tgcgcccgat cagactacga ctatcgtgcg tgcgaactct
    62461 tccaccacga cggccgctga accgctgaaa atgagctacg ttgaaggtct gctttccagc
    62521 aatcagaaag atgtgctgat ggaagagatc gtcgcaaact accacgctaa caccaaagac
    62581 gctgaagtcg ttctggttga aggtctggtc ccgacacgta agcaccagtt tgcccagtct
    62641 ctgaactacg aaatcgctaa aacgctgaat gcggaaatcg tcttcgttat gtctcagggc
    62701 actgacaccc cggaacagct gaaagagcgt atcgaactga cccgcaacag cttcggtggt
    62761 gccaaaaaca ccaacatcac cggcgttatc gttaacaaac tgaacgcacc ggttgatgaa
    62821 cagggtcgta ctcgcccgga tctgtccgag attttcgacg actcttccaa agctaaagta
    62881 aacaatgttg atccggcgaa gctgcaagaa tccagcccgc tgccggttct cggcgctgtg
    62941 ccgtggagct ttgacctgat cgcgactcgt gcgatcgata tggctcgcca cctgaatgcg
    63001 accatcatca acgaaggcga catcaatact cgccgcgtta aatccgtcac tttctgcgca
    63061 cgcagcattc cgcacatgct ggagcacttc cgtgccggtt ctctgctggt gacttccgca
    63121 gaccgtcctg acgtgctggt ggccgcttgc ctggcagcca tgaacggcgt agaaatcggt
    63181 gccctgctgc tgactggcgg ctacgaaatg gacgcgcgca tttctaaact gtgcgaacgt
    63241 gctttcgcta ccggcctgcc ggtatttatg gtgaacacca acacctggca gacctctctg
    63301 agcctgcaga gcttcaacct ggaagttccg gttgacgatc acgaacgtat cgagaaagtt
    63361 caggaatacg ttgctaacta catcaacgct gactggatcg aatctctgac tgccacttct
    63421 gagcgcagcc gtcgtctgtc tccgccagcg ttccgttatc agctgactga actggcgcgc
    63481 aaagcgggca aacgtatcgt tctgccggaa ggtgacgaac cgcgtaccgt taaagcagcc
    63541 gctatctgtg ctgaacgtgg tatcgcaact tgcgtactgc tgggtaatcc ggcagagatc
    63601 aaccgtgttg cagcgtctca gggtgtagaa ctaggtgcag gcattgaaat cgttgatcca
    63661 gaagtggttc gcgaaagcta tgttggtcgt ctggtcgagc tgcgtaagaa caaaggcatg
    63721 accgaaaccg ttgcccgcga acagctggaa gacaacgtgg tgctcggtac gctgatgctg
    63781 gaacaggatg aagttgatgg tctggtttcc ggtgctgttc acactaccgc aaacaccatc
    63841 cgtccgccgc tgcagctgat caaaactgca ccgggcagct ccctggtatc ttccgtgttc
    63901 ttcatgctgc tgccggaaca ggtttacgtt tacggtgact gtgcgatcaa cccggatccg
    63961 accgctgaac agctggcaga aatcgcgatt cagtccgctg attccgctgc ggccttcggt
    64021 atcgaaccgc gcgttgctat gctctcctac tccaccggta cttctggtgc aggtagcgac
    64081 gtagaaaaag ttcgcgaagc aactcgtctg gcgcaggaaa aacgtcctga cctgatgatc
    64141 gacggtccgc tgcagtacga cgctgcggta atggctgacg ttgcgaaatc caaagcgccg
    64201 aactctccgg ttgcaggtcg cgctaccgtg ttcatcttcc cggatctgaa caccggtaac
    64261 accacctaca aagcggtaca gcgttctgcc gacctgatct ccatcgggcc gatgctgcag
    64321 ggtatgcgca aaccggttaa cgacctgtcc cgtggcgcac tggttgacga tatcgtctac
    64381 accatcgcgc tgactgcgat tcagtctgca cagcagcagt aatctcgtca tcatccacag
    64441 ctttgcgctg cggatatctg aaccggaaat aatcactatt tccggttttt tattctctta
    64501 atttgcatta atcctttctg attatcttgc ttaactgcgc tgcatcaatg aattgcgcca
    64561 tctcactttg catacttacc actttgtttt gtgcaaggga atatttgcgc tatgtccgca
    64621 atcactgaat ccaaaccaac aagaagatgg gcaatgcccg atacgttggt gattatcttt
    64681 tttgttgcta ttttaaccag tcttgccacc tgggtagttc cggtggggat gtttgacagt
    64741 caggaagtgc agtatcaggt tgatggtcaa acaaaaacac gcaaagtcgt agatccacac
    64801 tcatttcgca ttctgactaa cgaagcaggc gaacctgagt atcaccgcgt acagctgttc
    64861 acgacgggcg atgaacgccc gggcctgatg aacttcccgt ttgaagggtt aacctcagga
    64921 tcgaaatacg ggacagccgt tggcatcatc atgtttatgc tggtgattgg cggcgcgttt
    64981 ggcattgtga tgcgtacagg aaccattgat aacggtatcc tggcgcttat tcgccatacc
    65041 cgcgggaatg aaattctctt tattcctgcg ctgtttattc tgttttcact tggcggcgcg
    65101 gtatttggta tgggagaaga ggccgtcgcc tttgccatta tcatcgcacc gctaatggtc
    65161 cggctgggct atgacagtat taccaccgtc ctggtgactt atattgccac gcaaatcggt
    65221 tttgccagtt cgtggatgaa cccgttttgt gtggttgttg ctcaggggat tgccggcgtt
    65281 ccggtgcttt ctggctccgg gttgcgcatc gtggtgtggg ttatcgccac tctgattggc
    65341 ctgatcttta ccatggtgta cgcctcacga gtgaaaaaga atcctcttct gtcacgcgtg
    65401 catgagtccg accgcttctt tcgtgaaaag caggcggatg ttgaacaacg tccgtttacc
    65461 tttggtgact ggctggtatt gattgtcctg acagccgtaa tggtctgggt gatttggggc
    65521 gtgatcgtta atgcctggtt tattccagaa attgccagcc agttcttcac catgggtctg
    65581 gtgattggca tcatcggcgt tgttttccgc cttaacggca tgacggttaa taccatggct
    65641 tcatccttta ccgaaggggc gcgaatgatg atcgcccctg ccctgctggt gggtttcgcc
    65701 aaagggattt tgctgctggt tggtaatggt gaagcaggta atgccagcgt gttaaatact
    65761 atcctcaaca gcattgccaa tgccattagc ggtctggaca acgcggtcgc ggcctggttt
    65821 atgttgctct tccaggctgt atttaatttc ttcgtgacgt ccggttctgg tcaggcggca
    65881 ttaactatgc cgttactggc accgcttggc gatctggtcg gtgttaaccg tcaggttacc
    65941 gtgctggcgt tccagtttgg tgatggcttc agccacatca tttacccaac ctcagcttcg
    66001 ttaatggcaa cgctcggtgt gtgccgggtg gacttccgta actggctgaa ggtgggcgcg
    66061 accctgcttg gactgctgtt tattatgtcc agcgtcgtag tgatcggcgc tcagttgatg
    66121 ggctaccact aaaaatgtta agagccgcaa ttgcggctct tttttcattc tgccgtttca
    66181 gtctctacgg cttcattttt ggcattgcgc ttcatccaca acgccagcgc tttcagcgag
    66241 tctggagtga actcatcgca gcgtgcggtg atttcttccg gcgtcagcca gcaaacttca
    66301 ctgacttcat cttcctgtag ggcgaaggga ccgtgagaga cgcaactgaa caacgcgccc
    66361 cagacacggc aatttttatc ttcgaaatag aactgcccgt gctcggcaaa ggggacaccg
    66421 gcaatgccca actcttcttc cgcttcgcga cgcgcggatt ccagcagttg ctcatcggcc
    66481 tgaactacac cgcctgcggt cgcatctaac atgccgggta aaaagtcttt tgtctcggta
    66541 cgacgctgga ccagaatttt gcccatgcca tcatgcacga cgatgtaagt tgcacgatga
    66601 cgcagacact gtgcccgcat ttgttcccgg ctggcttgtg caatgacttc gttctcttca
    66661 ttgacaatat ccacccattc agtacttgcc aaacgacgct gttccattat caggaaacct
    66721 tctttttctg gcgctcttac ggcgcatttt gagttgtggg taaattacgg attaatcgcg
    66781 acctgcgcaa tgatactttg atcattgagt gcgattacac taagtacgtc attatccagc
    66841 atgccataac tcgccggatt accgcctttc ggaatactca ccgagccggg gttgaagtgg
    66901 aaaatctccc cccgctgttc tgccactggc agatgggtat gaccgtacac cagcacatcg
    66961 ttctggttta aagcaggtag attttccggg ccaaaaagat gaccatgcgt caaaaacaga
    67021 cgttgttttt ccagtaatac ctgttgccac ggcgcggtta tcgggaaatg cagcatcatt
    67081 tgatccactt cgctgtcgca gttgccacgc acagcgataa ccttatgtgc cacttcatta
    67141 agccgttcag cgactttggc tggcgcgtaa ccctccggta aagcattacg cgggccgtga
    67201 ttcaacacgt cgccaaggat caccagccac tgggcaccgc tttggacaaa caactccaca
    67261 acacgttccg tcgccggtaa cgacccatga atgtccgatg caaacatcag tttcatcact
    67321 cactcctcgt cgaaaaaacg taagctatga tactggattc tgtcgttgct atcagccaga
    67381 ttgcttcgcc gaaagtgcaa taaaacgctg gacagacgct cgctgccact ggaatgtcgc
    67441 ataatccacc aggcgttctg gcacctcatc gccatgtagt accaggcggt taatcattag
    67501 cgccagatca gtatcagcaa tgcaccattc accaaataaa ttcggctgac ccagtgctaa
    67561 caaatgttct gccatcgcga acagtttctc tgcactggct tttccctcgg ccgttagtgg
    67621 cgcttttttc gcccccgcaa agacaacatc cgtcggacgc tcttcgcgga tgggcatcag
    67681 atcgctgcgc agccaggcct gaatctgtcg tgcacgcgca cgattttcta aatcaagcgg
    67741 ataaatacgt tcccaggtcg gtggcgcaaa tcgatcttcc agatactccg caatggcaga
    67801 agattcactc aactcaaaat catcgatttg taataacggc acacggcgtg tctgaccgta
    67861 accttgccac gtcggctgca aatgttcacc gctgtcgagg tcgatggtct tgatatgaaa
    67921 cgacaggcct ttttcctgca acgccaccca ggcggataac acataagggg agaaaaagtg
    67981 ggcatctgac caaagcgtga tagcgggttt actcattatg tcctcggcaa taaagggggc
    68041 tggctatcaa actatagagc ctttttgtca ctgtcacgtg acgaatactc acgtaagtcg
    68101 ccgcgctttc tatacttgtg gtgttcagat gattaaacaa ccggagctgc aatgatcgat
    68161 ctctatttcg ccccgacacc caatggtcac aaaattacgc tgtttctcga agaagcagag
    68221 cttgattatc gcttgattaa ggtagacctg gggaaaggcg gtcagtttcg tccggaattt
    68281 ttgcgcattt cgcccaacaa caaaattccg gcaattgttg atcattctcc tgccgatggc
    68341 ggcgaaccgt taagcctctt tgagtctggt gccattttgt tgtatctggc tgagaaaaca
    68401 ggactctttt tgagtcatga aacgcgtgag cgcgccgcca cattacagtg gttattctgg
    68461 caggtaggcg gactggggcc gatgcttggg caaaatcatc attttaatca cgcagccccc
    68521 caaaccattc cttacgctat tgaacgttat caggttgaaa ctcagcgtct ttaccatgta
    68581 ctaaacaagc ggctggaaaa ctcgccctgg ctgggaggcg agaactacag cattgcggat
    68641 attgcctgct ggccgtgggt taatgcctgg actcgcctgc gaattgacct cgcaatgtat
    68701 ccggcagtca agaactggca tgagcggatc cgttcgcgcc ctgccaccgg gcaggcactg
    68761 ctaaaagcac aactcggtga tgagcgttcg gatagttaac agaaacaggt tctcgtgtat
    68821 tatttcatcc taagtaaaac aacggagaac ctgcaatggc acaacctgcc gctattattc
    68881 gtataaagaa ccttcgtttg cgtacgttta tcggaattaa ggaagaagaa attaacaacc
    68941 gtcaggatat tgttatcaat gtgacgatcc actaccccgc cgataaagcg cgcactagcg
    69001 aagatatcaa cgatgcgctg aattatcgca ccgtaacgaa aaacattatt cagcatgtag
    69061 agaataaccg tttctctttg ctggaaaaat taactcagga tgtgctcgat atcgcacgtg
    69121 aacatcactg ggtgacgtat gctgaagtgg agatcgataa actgcacgcg ctgcgctacg
    69181 ccgattcggt atccatgacc ttaagctggc agcgttaatc gccatatcgg gaggctgcat
    69241 gaacatagtg atcaccggag ggacgggatt aattggtcgc catttgattc cgcgtttgct
    69301 ggagctgggc catcaaatta cggtagtgac gcgtaacccg cagaaagcca gttccgttct
    69361 cggccctcgg gtgacactat ggcaagggct tgccgatcaa agcaacctca acggcgttga
    69421 tgcggtaatc aacctggccg gagaaccgat tgctgataaa cgctggactc acaagcaaaa
    69481 agagcgtctc tgccaaagcc actggaatat cacgcaaaaa ctggtcgatt tgattaatgc
    69541 cagcgacacg ccgccgtcgg tactcatttc cggctcggca acgggctatt atggcgactt
    69601 aggtgaagtg gtggttaccg aagaggaacc gccgcataac gaatttactc ataaactctg
    69661 cgcccgctgg gaagagattg cctgccgggc gcaaagtgac aaaacgcgag tgtgcctgct
    69721 gcgaaccggc gtggtactgg caccggacgg cggtattctc ggtaaaatgc tgccaccgtt
    69781 tcgtcttggc ctgggcgggc cgattggttc cggtcggcag tatctggcct ggattcatat
    69841 tgatgatatg gtcaacggca ttctctggct gctggataac gagctgcgcg ggccatttaa
    69901 tatggtttcg ccctacccgg tacgcaatga acaatttgcc catgcgctcg gtcatgcgct
    69961 gcatcgcccg gctattttgc gcgtccccac aaccgccatt cggctgttaa tgggcgaatc
    70021 ttcagtactg gtattaggtg gacaacgcgc gctgcctaaa cggctggaag aagcgggttt
    70081 tgcgtttcgc tggtacgatt tagaagaggc gctggcggat gtcgttcgct gatgtggttt
    70141 acagcaaaca tccgccagtt aactccctgt gttacaggat tagtggcttt gcgcgataag
    70201 atcgtctggt gaaagtcggg tcaccatcat aactaactct ctgtctaaac ctctatccag
    70261 catctcctga gcaatacgca gggcttcttc gtgtttgccc tgcattgcgc cttcttcacg
    70321 taatctgtca gcaatggtca tcagtttctc cttttcttgt ggtgcgcgtt ctgttatctc
    70381 accaataaat gcacggaaac gctgggcatc ccctgtttgt aatacgtaat taaacagggc
    70441 ttttagctgt ctgtcattag tgttccctgt aactagcagc gaaacaattt ggtcgactaa
    70501 tcccaacaga tcgcgctgac gaatatgttt ctgaattaac tccaacagcg ccattttgcg
    70561 gtgttgcata atctcgtcat ccggcaccac ggcaatatcc accaacggaa acgctgatga
    70621 atatattttg cgggctatag caggctcggc aaattcatca agccagcaga gtgaataagg
    70681 ataaggactt ctgcaaccat gataaaacag catcgggatc accaatggaa gctctttata
    70741 gcccgcatca agatggtttt gcattgccgc aatggaataa cgcatcatgc gaaaagccat
    70801 taattcttcc ggcttacttt ggtgctctat cactacataa atataaccca ctccctcctg
    70861 cgttttcaca gaccacaaga ggtcggaata atattgccgc aggtcttcat caataaaact
    70921 gtttggttct agtttaagcg tcgttaaatc acacagtttg cgcagcggcg cgggaagatg
    70981 aatattaata aaattccgcg cggtgtctgg atggcgtaaa aaagatttaa ataccgcatc
    71041 atgcggcgtg gaagttgtcg atattgtcat ggcgttccgt cacccctcaa atttaagatg
    71101 acgcgacaat aaccacgatt aacacccaat gcagcaggca gtttttatct ttggacagcg
    71161 aattcagaag tatttttagc ggactgcaac ttttacattt cagcggaagc tgactcgcaa
    71221 attccccttg aaaatactcc ggcagcgtta gccaccggag aggaaattat ttcagcgatc
    71281 ccttaaggaa ctgttgcaga cgagggcttt gcgggttgcc aaataactgc tccggcgcgc
    71341 cctcttcttc tattttcccc tgatggagga aaatgacgtg agtagaaaca tggcgagcaa
    71401 agcccatttc gtgagtcact accaccatgg ttttcccctc ttctgccagt tgctgcataa
    71461 tacgtaacac ttcgcctacc agttcaggat cgagcgccga ggtaggttca tcaaacagta
    71521 aaacttctgg ttccatcgcc agcgcccgcg cgatagaaac acgctgttgc tgaccgccgg
    71581 aaagatgcac cggatatttc ccctgcgcac gttcgtctat cccgactttc gccagatact
    71641 tcaccgcccg ctcgcacgct tcctgcttgc tcaggcccaa cacctgaatc ggcgcttcca
    71701 tgacgttttc caacaccgtc atatggctcc agagattgaa gtgctggaat accatcgtca
    71761 ggcgtgtgcg cagtaagcgc agttgatttt tatcggcgac tttgagttga ccgtctttgt
    71821 cgcgcaccag attgatcgtc tggccattga ccacgatcga tccttcactc ggtttttcga
    71881 ggaagttaat gcagcgcaga aaggtacttt tccccgatcc cgacgatccg atgatgctta
    71941 ttacatctcc ggcattcgct tgcagtgaga cccctttcag cacttcgtgt tcgccatagc
    72001 gtttgtgcaa atcgataacg tttaatttat tctcggacat cgtattctca gtgcgttgaa
    72061 gaaggtttca catgctgcaa ccagcgtttt tccgctctgc gaaagaggct gatcaggaca
    72121 taagagatga ttaaatagag caccgcggca atgccgaagg cggtaaaagg ttgatacgtg
    72181 gcggcgttaa tatcgcgggc tattttcagc agatccggca cagtggccgt aaacgccagc
    72241 gcagtagagt gcagcatcag gatcacttcg ttgctgtatg ccggtaacgc aatacgcagc
    72301 gcagaaggca aaataatgca gcgatacatt ttaaaggtcg agaagccata ggctctggcg
    72361 gcttcaattt ccccatgcgg aaccgaacgg attgccccag caaaaatctc ggtagtgtaa
    72421 gcgcaggtgt taagcgtcag cgccagcacg gtacagttca ggccactgcg gaagaaagcg
    72481 ttaaggaatt cggttccctt aacaatctca agcgtgtaca tgccggaata gaacaccagc
    72541 aactgaacat acagcggcgt accgcgaaaa atataggtaa ataaccagat tggaaactgg
    72601 atgtatttat tactggagac acgaccaatc gccagaaaca gcgccaggac tccgcctatc
    72661 actaccgaca aaataagcag ccacagagtg atcgccacac cagtaaagcg ataaccgtcg
    72721 gtccacagca gcggtttcca gtattcatgt aagatttcga tcacaggtca gccctcttca
    72781 cacccacgga gtagcggcgc tcaaggaaca gcagcacacc attggaaacg gtggtgaaaa
    72841 ccaggtaaat cacgccacag acgatggcga aatagaacgg ttcccaggta cttttgcctg
    72901 ccagctgcgt ggctttgacc acatcttcca ggccgagtaa cgaaaccagt gcggtagatt
    72961 tgaggatcac ctgccagttg ttgccaatgc ctggcagggc gtaacgcatc atcgccggaa
    73021 acatgatcct ccgaaacact tgcccgcgag taaaaccgaa cgccgtcgcc gcctctatat
    73081 gccctttcgg cactgccata aacgcgccac ggaacgtttc ggtaaaataa gcaccgtaga
    73141 taaaaccgag agtgataata ccagcgacca tcggatcgat atcaatctgc ccgacgccca
    73201 tcgcctccgt caccatgttt agcgcaatct gcaaaccata gaaaatcagc agcatcaaca
    73261 ccaagtccgg cacgcctcga atcagcgtgg tgtacccttc gaaaatcagc cccaaaagcc
    73321 gattttgcga gagcttaccg ccagcgccaa ttaaaccgat gattacagcg agcactacag
    73381 agctgatagc cagctccagc gtgacgagcg caccctgtaa aataacacct gaaaacccat
    73441 acaacatgct gcctgtcctg tcgtgtgtgg tgaattaccg cctcttgtct ccctccggtt
    73501 acccgaaggg agcgggcttt ctttcaccga tggcgattaa ccaccataaa catcaaaatc
    73561 gaagtacttt ttcgctaatt tctcgtaagt accgtcagcg cgcatttcgg caaaggcttt
    73621 gttcagtgcc tcgcgcagtt cgttatcttc tttacgcagg cccatgccgg tccctacgcc
    73681 aaacagtttt tcatctttaa cagacggacc accgaatttg taatctttac cgacgggttg
    73741 tttgaggaaa ccttcgctgg cagcgacctc atcctggaac gcggcatcaa tacgtccggc
    73801 agtcaggtca gaataaatgt tgtcctgccc ctgatacgag acgatttcaa tgccttttgg
    73861 tgcccagtgt tcattaccga acgtctcctg ggtggtgccc tgcaatacgc ctacccgttt
    73921 gcctttcagc gactcgactg tcggctgaat gtcagaattt ttcgccacca ccagacgaga
    73981 atcggcagcg tacagtttgt cggtgaaggc aatttcttgc tgacgttttt ccgtaatgga
    74041 aagcgatgac atgatggcgt caatcttctt cgcttttaag gacgggatta acgcatccag
    74101 cgggttttcg acaaacgtac attgcgtatt gatgcgcttg cacaactcct tcgccagctc
    74161 gatatcgaaa ccaaccagtt cgccttgtga attttttgat tcaaatggcg cataggtcgg
    74221 gtcggtaccg atgcggatgt tttgcggaat tgcagcaagt gccgcagttg cgctggagaa
    74281 ggccagaacc agagagagcg atagcaccag ttttttcata atgtcctcaa ctgacagtct
    74341 ttttataggg aatatttaca ggtttgttac acttatcgtg ccataaaatt gaccatcaag
    74401 gcaaaatatc ccgccccgtt agacgtatta tttttcatta aaaatgctta agtatgcatt
    74461 tcaatgcacc acgatagtgc aacaaacctg gcgtgcacca tgatgatcat tccatcaggt
    74521 acagcttccc agcgacgtat cagtcaccgt agacattaaa gtcgaaatac tttttcgcca
    74581 tcttgtcgta ggtgccgtcc tgacgcagct cgccaagcgc cttattgaag gcagccgtca
    74641 gttcagcatc atctttacgt agccctacac cggtgccatc accgaagtat tttttgtctt
    74701 ttactgatga gccagcaaag gcgaaatctt taccagcagg ttgcttgagg aatccttcgc
    74761 tggcagcaac ttcatcttgt aacgcagcat ccagacgtcc tgcagccaga tcggaataga
    74821 ccaaatcctg gttggcatag gccaccacat ccacgccttt actacgccag gtctcgttag
    74881 cgtaagcttc ctgggttgat ccctgcagca caccaacatg tttacctttc agtgaatcca
    74941 gcgttggctg aatcggtgaa cctttggcag caatcaaacg agaatctgcg gcgtacagct
    75001 tgtcggagaa ggcaatctcc tgctgacgtt tatcggtaat ggaaagcgac gaaataatag
    75061 cgtcgatttt tttcgctttc agtgagggga gcagcgcgtc aaagtcactg gcaacccagg
    75121 tacatttcac ctgcatccgt ttgcacatct cgttaccgag atcgatatca aagccaacaa
    75181 aatcaccttt agcatctttc gacgagaacg gcgcataggt ggtatcggtt ccgatacgta
    75241 ccgtctccgg tagcgccgca tagctggaag ccgctgcgga gagaccgact aataaagaca
    75301 gagcgagaat cgacttcttc atacataacc ctcaagtgaa aatggctttt ttatgttgtg
    75361 tattgtgttg tgtgtttgca ggctctttca tgcaggtctt atgccatctt gacgacaaca
    75421 gtaacattca acgttaaata tgttaataag acgttgcatt attgtcctga agttgaagat
    75481 agcaggtata gcggttgaat cgcagcgttt cgattgaatg gcagaaacaa aatgtcgagg
    75541 atttgatcgc ggttgcaaaa ttgccctgaa acagggcaac agcggagtta tgcgccctgc
    75601 cagcgggcaa agagatcttc aggaagggtt attgcaaact ggtcaagaac acgattaacc
    75661 gtctgattta tcacatcatc aagggattgc gggcgatgat aaaacgccgg aacgggaggc
    75721 ataatcaccg caccgatttc tgccgcctga gtcattaaac gcagatggcc taagtgcaat
    75781 ggtgtttcac gcacgcagag caccaacggg cgacgctctt tcagcaccac atctgccgca
    75841 cgggtcagta agccatcagt atagctatgg acaatgccgg acagggtttt gattgaacag
    75901 ggtaaaatca ccatccccag cgtctggaaa gaaccggaag agatgctggc ggaaatatcg
    75961 cgcgcatcga gcgtgacatc agctaatgcc tgcacttcgc gcagggaaaa atccgtttcg
    76021 agggataagg tctggcgcgc cgcctggctc atcaccagat gcgtttcgat atctgtgaca
    76081 tcgcgcagaa cctgtaataa gcgcacgcca taaatcgcgc cgctggcacc gctgatgcct
    76141 acaatgagtc gtttcataaa tagttgccct gtcagacttg caggcagact ttgcagtatt
    76201 tcggcgggag ttgcaagtca gggtgccaga ccggcaccct cagcgaaggc atcatccttc
    76261 gttatgcatt tcgagatttt ccacttcgtt ctgacgttgc actgctttgg cgtcatcatt
    76321 acgtaacgta tcgaggaaat cgaggtagcc ctgatcaaca tctttggtga cgtagacgcc
    76381 gttgaacacc gagcattcaa actgctggat atccggattt tcagcgcgaa cggcgtcgat
    76441 cagatcgttc agatcctgga aaatcaaccc gtcagcaccg atgatctggc gaatttcatc
    76501 aacttcgcga ccgtgagcga tcagttccgt ggcgctcggc atatcaatac cataaacgtt
    76561 cgggaagcga atttccggtg ccgcagaagc gaggtacact ttcttcgctc cggcttcgcg
    76621 tgccatctcg ataatctgct cagaagtggt gccacggacg atggagtcgt cgaccagcag
    76681 gacgttttta tcgcggaact cggcgcggtt ggcattcagt ttacggcgca cggacttacg
    76741 acgcagctgc tggcccggca tgataaaggt gcggccaaca tagcggtttt taacgaagcc
    76801 ctggcggtac ggtttgccca gaatacgagc aatttccagc gcgatatcac acgaggtttc
    76861 tgggatcggg atcaccacgt cgatatccag atcttcccat tcgcgggcaa ttttctcgcc
    76921 cagtttcgtg cccatattca cacgcgcgct gtaaacggaa attttgtcga taaacgagtc
    76981 cgggcgggca aagtatacat actcaaacag gcacggattg ctgaccggat tgtcagcaca
    77041 ttgacgggta aacaactgcc cttcttcagt gatgtaaatc gcttcgcccg gcgcgacgtc
    77101 acgcaggaaa tcaaagccca gcgtatcgag cgctacgctt tcggaagcga ccatatattc
    77161 tgtacggttc tcgtcaatat cacgttttcc cagtaccagc ggacgaatcc cgtttggatc
    77221 gcggaaagca accataccgt ggccgataat catcgccaca caggcatacg cgccgcggat
    77281 taagcggttt gtggcagcaa tggcagcgaa aatattgtcg gcttccagcg ggtagtggcg
    77341 gaagttgtcc agctcgctgg cgaagatatt aagcagaatt tccgagtcgg aagtggtgtt
    77401 gatgtggcgg cgtttttctt caaacagttt tttacgcaac tcgtgagcgt tggtcagatt
    77461 gccgttgtgg gcaagcgtaa tgccatacgg ggagttaacg taaaacggct gcgcttcaga
    77521 ggcgctggag ctgccagccg tagggtaacg cacatgacca atgcccatat tgccctgcaa
    77581 acgctgcata tggcgagctt caaatacatc gctcaccagc ccgttcgctt tacgcaaacg
    77641 gaagcagtta ttggcatcta tggtgatgat gccggcggca tcctgaccgc gatgctgaag
    77701 caccgttaag gcatcataaa tcgactggtt aaccggcata acaccggcga taccgacaat
    77761 accgcacata cgtctttttc ctcgttaagc cacatctcag agcacttacg ctctgggcaa
    77821 gaaacttgac gagctttgca gataatcaaa aaaccatctg atgataaaac tgaattgcgg
    77881 gatcagctgt gatttgctcc agtcttcact tttcgacacc ccggtaaagg agtcgagaaa
    77941 gaagagaatg gcggcaacaa tcaacacacc gcgcaacgca ccgaaacaga cgcccagcac
    78001 ccgatcggtg cctgacaacc ccgttttctc caccaactgg cctatcacga agttcacgat
    78061 agcaccaacg atcagggtag cgataaacag taccgcgatg gcaatcccat ttcgaaccag
    78121 ttcgtcttca aagcccgtaa accagactga caggtaagtg tagtaatgac tggcaacaaa
    78181 gaaagcacaa ccccatgtca ccagcgataa cgcttcacga acaaagccgc ggatcaggct
    78241 aaccagagag gaaaaagcaa tcaccgcgat tatggcgtaa tcaatccaga ccatatgtgt
    78301 cccacgattt tacgccctgt catcctgttc ggggcgcatt ctaacagaaa aagaaaacgt
    78361 ttgcgtaggg atttccttcc cgcgcatcaa taaaaatggc gctgaaaaaa tattcaacgc
    78421 catcgacttt ttatgccttt gcggcatcgt gcaatgcgtg tcggatgcgg cgtaaacgcc
    78481 ttatccgacc tacggttcta acactgccca agcctgataa gacgcgccag cgtcgcatca
    78541 ggcaagactg tgttaattcg gcgtatagcc cattaccacg ccacttaagc cagaaagttg
    78601 cttcaactca cccagcgaac ctttcagctt atctttcgag gcatccggcc caaccagaat
    78661 acgggtaatt ttaccctgca ctggcgtgga tggcgacgta taaacccgat aaccggcacc
    78721 gcgcagctta ccgacaatct cattcacttt atcggcattt ttcagcgcac ccagttgcac
    78781 aacataggct ttacccgttg gcgctgcttt ttcttccacg accggcttcg gttctggctt
    78841 cggcgcaggt ggtgcttcaa cctttggttt aggcggctcc accggtttcg gcttcggtgg
    78901 ggcgaccggt gcaggttccg gttcaaactc ggtgttattg gctgcaatag tggccggatc
    78961 gagcgacggt gctgcggcat cacctgcccg cacctcttcc gctgcgcctt ccggcggctg
    79021 cgtcggcaac gcctgggtgg cggctggcat catatcaggc tcatcacgat cgcccgcttt
    79081 cggcaccagc gggatagccg cgaactcatc ctgataatgt tttttctgcc cgtccagcag
    79141 ccctggaagt acaatcaccc ccagcgccac cagcacgatc gtgcccacta accgattctg
    79201 aaacttactt gccaccgctt ctcctcgcgt caatcacttc catgacatgt gcgaccgtgt
    79261 ggaaagaacc acacaccagc acggtgtctt ccgctttagc gtccgccatt gcggcatccc
    79321 atgcctgcgc aacgctatca aatgatttgc cgttacccaa atgctcaagc agttgttctg
    79381 ccgtggcacc gcgcggccct tccagtggcg cacaatacca gtcatcaacc acgcttttca
    79441 accaggccag agttccggca atatctttat catgtagcat accgataacc gccagcacgc
    79501 gcccgttttt cggtagcgct ttcatacgcc cggtgagata ttccgccgca tgtggattat
    79561 gcgcgacatc aaaaataacg cgtggcgact cgctcacaat ctggaaacgt cccggcaaaa
    79621 ttgcgctggc aatcccgtcg cgaatggcat tttcactgac ttccagcccg ctggcacgca
    79681 gtgccgccag cgccgttgcg gcattcggtt gcgggacaag cggcaacggc agattttcca
    79741 gcgtgccgtg agcatcgcta aacgcccagt catgatcggt gacggaatag ttccactcaa
    79801 cgccccgacg ttgtaacagt gcaccttttt cctgcgccac atcagcaatg gtagaaggca
    79861 tttccggctc accgacaatt gccggttttt cgctgcggaa gatgcctgct ttctcgcgac
    79921 caatactttc gcgatctgga cccagccagt cggtatgatc cagcgcaata ctggttacta
    79981 ccgcggcatc ggcatcgaca atattggttg cgtccagacg accgcccagc cctacttcca
    80041 gaatcaccac gtcaagttgt gcctgcttga acagccacaa cgccgacagc gtaccgtact
    80101 cgaaataggt cagggaaata tcaccgcgtg ccgattcaat ctccgcaaaa gaggcggtgt
    80161 gggccgattc cggcaattcc cggccctgca cacgtacgcg ctcggtataa cgcaccagat
    80221 gaggcgaact gtagacgccc actttgtacc ctgccgccat cagaatcgac tccagcgtac
    80281 ggcaggtggt gcctttgcca ttcgtacccg caacggtaaa cacaaatggc gctggtttca
    80341 ggacgccaag acgcgccgcg acctggctca cgcgctcaag gccgagctcg atagttttac
    80401 tgtgcaggtt ttccagataa gaaagccacg aagccagagg cgacgcggct tgaggagtgc
    80461 gtttgataat catggtatcc gctgattcgt tacggtgaga atagcaaaag ggcagagcca
    80521 gtggccctgc ccttatcagt tatcaggcct caggttcctg atccggtacc gggggtacca
    80581 ctacgccttc acgcggcgct tcaggattcg gcgctggcag attcatcaac ttcgccagaa
    80641 tgctcgccag tttcaggcgc atttccggac gacggacgat catgtcgatc gcgcctttct
    80701 cgatcaggaa ttcactgcgc tggaatctag gcggcagttt ttcgcgaacg gtctgttcga
    80761 taacacgcgg accggcaaag ccgattaacg ctttaggttc agcgatgttg agatcgccca
    80821 gcatggcgaa acttgcagaa acaccgccca tcgtcgggtc ggtcagcacg gagatgtacg
    80881 gcaagccgcg ctcctgcatt tttgccagtg ccgcagaggt tttcgccatc tgcatcagcg
    80941 acatcagtgc ttcctgcata cgtgcgccac cagaggcgga gaagcagatc agcgggcagt
    81001 tatcttcctg cgcctgctca acggcacgca cgaaacgtgc acccacaaca gaccccattg
    81061 aaccgcccat aaaggcgaac tcgaatgccg cagcgacaac cggcattcca tacagagtgc
    81121 ctttcatcac caccagcgca tctttttcgc cggtttcttt ctgcgcagat gccagacggt
    81181 ctttatactt cttggagtca cgaaacttca gctcatcttt cggctcaagc tcgctaccca
    81241 gctccacaag gcttccttca tctaacaggc tatgcaggcg attacgcgct gtcatacgca
    81301 tgtgatggtc acacttcgga cagacctcaa gattacgttc cagctcagcg cggtataaaa
    81361 cctgaccgca gctatcacac ttagtccaca ccccttcagg aatgctcgcc ttgcgggtgg
    81421 gagtaatgtt gcttttaatt cgttcaatcc agctcattag ggacctttct gtctgaactt
    81481 ggttcgatgc cagttttatc tttggggacg cataatgcca tttttgcccc caacagacca
    81541 tgaatgttgc acattaaaac ataacagccc gaaactttgg ataaaaaagt ggtcgaaccg
    81601 cggagttact ttttattttg cggcgcgtgc cgcagcgcgt ttgtgacgga ttatttcgat
    81661 gacgcccggc aaaatagaaa ccacaataat cccgacgatc agcagcttaa ggttatcctg
    81721 aaccatcggg attgtaccga agaaatagcc tgcgtaggta aaaagcagta cccacaacag
    81781 tgcgccgatc acgttataag cagcgaaatg acggtacgac atgtggccca ttcccgcaac
    81841 aaacggggcg aacgttctga cgatcggcac aaaacgggcg agaataatcg ttttgccgcc
    81901 atgtttctca taaaactgat gggttttgtc gagataactg cgacggaaaa ttttcgaatt
    81961 gggattactg aataactttt caccgaacaa ccgcccgata gtgtaattga ccgcgtcacc
    82021 cacaatcgcg gcgatcagca tcagcactac catcatatgg acgttgagat cgttggtttc
    82081 cagtgatgcc aacgctccgg caacaaacag caatgaatca cccggtaaaa acggcattac
    82141 caccagaccg gtttcacaga acaaaattaa gaacaaaatg gcataaaccc agacgccgta
    82201 ctccgcgact agctctgcca gatgcacgtc aatgtgcagg ataaaatcga tgaggaaata
    82261 aatcaggtcc atatttgcct atgccttgta ctcgtcatct ttcaggctgt aactacgttg
    82321 gctacagcct gaaatgttcc gagcattatt ctcgttagtc cgccagaaat agcgggccca
    82381 ttggcggttt tggaagatca taccggtcag ggtaatccac cgcgaccaga tacagccctt
    82441 ccgcttttgc cgttgctgcc gccagcgttc tgtcctttgc cgccagcaac tctgctatcc
    82501 agctctccgg ctggttgtgg gcaccgactt ccatcaggct gccgacaata ttcctgacca
    82561 tatgatgtac aaaggcattc gctttgatat caaccaccac ataaggaccg tgacgcgtga
    82621 cattaatatg cataacgttg cgccacgggg ttcgggactg gcactgcacc gcacggaacg
    82681 aggtgaaatc attctcgccc agcaagcatt gcgcagcccg atgcatccgt tcagcgtcca
    82741 gcggttcgta aaaatgggtt acccctttac tcagtaccgc cgggcgcagc cgatgattgt
    82801 agatgatgta gcgataacgg cgagccgtgg cgctaaatcg ggcatgaaaa tcatcaggta
    82861 cagctttaac ccaacgcacg gcgatgtcac caggtaaatt cgcatttacg cccaaggtcc
    82921 acgccgcgtc tttgcgctgc gcggttgttt cgaaatgcac cacttgcccg gtaccgtgta
    82981 cccctgcgtc agtacgcccg gcgcagaaga cggtgatggg ttcgttcgcc acctgggaga
    83041 gcgccttttc cagcttctcc tgcacactgc ggacttcatt ctgccgttgc cagccgtaat
    83101 acttactgcc gtcgtactca atgcccagcg caattttata aactggcagt tgttgctggt
    83161 cggacattag tacagatact cctgcaccag tttctcggcg atttttactg ccatcagcgc
    83221 gccgccaaag cgaacgttat cggccaccga ccagagctgg acttgctccg gcataccgta
    83281 gtcattacgc acgcagccaa cagaaagatg cggcgtaccc gaagcatccc ctacctgagt
    83341 cgggaattcg ttctcttcag agagcacaat atcttcgccc tgagcaaacg catcacgcgc
    83401 ttcttctgcc gccagcggac gcagtgcttc aaagttgacc atctgggcat gaccgtagaa
    83461 taccggtgcc tggacgacgc ttgccgaaat catcagccct tcgtcctgca ggattttgcg
    83521 tacttcgtca acgatacgac gttcttcacg cacgctacct tcgctatccg gcaacaacgg
    83581 cagcatattg aacgccagtt gacgaccgaa gaaatcttct tcgtcaatcg gaatgccgtt
    83641 gagcaatttc gcactctgcc ccgctaacgc atcgaccgct tttttgccct gggcggaggc
    83701 tgaaatcagg ctggtaacac tgatacgcga caaaccgccc tgatcgatta acggtttcag
    83761 tgccgccagc agctggctgg tcagactgtc tggtacggcg atgacattcc ggttgcggta
    83821 atcggtcagt acaaacgggt ttacttccgg caccaccagc ggtacgtcgg gttcgagagc
    83881 aaacagtccg ctgctgtcga tcaccaggca acctgagttg gtcgcttctt caacccaggc
    83941 agcggtagct tctttgcctg cgacaaaaaa tgccagctgc gcctgcgtcc agtcgaattc
    84001 agcggcatcc tgcacggtga ttgttttacc accaaagcgc agttgttcgc ctgcgctttc
    84061 gttacgtgcc agtgcataaa tttccccaac cgggaactga cgttcagcca gcgtttcaag
    84121 cagggcttcg cccatagcgc cagttgcgcc caggacggca atgttccagc cttcagacat
    84181 ggtggtttac tccagaaata gcaaagctcc ctgccaatgt tacagcagag agcatgaaga
    84241 agagattaac gtgccggatg atgaacggcg ttaaaaccca gtttacacag caatgatgcc
    84301 gcactggcgt catcacaaat tacatacaga gatgaccatt cacggcgctc aagatagttt
    84361 ttgcgcagtt tatcgaactc acccggtatc ccggcgactt tacgcagcgg tgcgtcatcg
    84421 cggcgcacat catacaccaa atgcaccagc cttttcagcg tcggttgatc gagcggacca
    84481 tgcagcgtaa tgcggccaaa ctcaggcgcg ggcagcaacg tatccagcgc aacgtgttgt
    84541 tcatgcccaa taaacttgct ataggcttca aacacttgcg tcgtgccacg cgctttaccc
    84601 tccaaggtat agcctgcgat atgcggcgtg ccgatatcca cttttgtcag caattccaca
    84661 ttaagttccg gctcgccttc ccagacatcc agcaccacgc ttaacttctg gccttcattc
    84721 aggcaggtca gcagcgcggt attatcgacg actgcgccac ggcaggcgtt aatcagaatc
    84781 gctccgggct tcaggctacg gatcagtttt tcatccgcca gatgtagcgt tttgtacgga
    84841 ccatctttaa agagtggcgt atggaaagtc agaatatccg cgcgctggac taactcatcc
    84901 agcgagcgga aatcaccctc atccccacgg tcggcgcgag gcggatcgca aagtaaggtt
    84961 ttaatcccta acgcttccag tcgcgcctgc aatcgacgtc caacgttacc aacgcccacg
    85021 atccccaccg tacggtcgtg cagtgaaaat ccatcgcgtt cggcaagcat cagcagggag
    85081 gagaaaacat attccaccac cgcaatcgca ttacagccag gtgcagcgga aaaaccaatt
    85141 cccgcctgct tcaaccatgc ttcatcgaca tggtcggtcc ccgcagtggc agttccaaca
    85201 aatttaatgg gttttcctgc cagcaaagat tcattcactt tcgtgaccga acgcaccatc
    85261 agcgcatccg cgtctgccag ttgagcgacg gggattggac gcccgggaac cgcgatcacc
    85321 tcacccaaac ggctaaataa gtcgcgggca taaggcatat tttcatcaac aaggattttc
    85381 acgtttgtgt tacctgtatg agacgagagt taaccggaca agtgtgccat aatctcgcgg
    85441 ccaggcatac ttgcgaagat ttcaggtata aggatacgta atgatacaac ctatttccgg
    85501 ccctcctctt gggcagccac caggtcaggg agataaccta ccgtctggcg cgggcaatca
    85561 gcctttatcc agtcagcaac gtacttcgct ggaaagctta atgacgaaag tgacctcact
    85621 gacgcaacaa caaagagcag aactgtgggc gggtatcagg cacgatattg gtctgtcggg
    85681 agattcaccg ctgctttcgc gtcacttccc tgccgctgag cataatctgg cgcaacgtct
    85741 gctggccgcg caaaaaagcc attctgcccg ccagctttta gcgcaattag gggagtattt
    85801 acgtctgggg aataatcgtc aggcggtcac ggattatatc cgtcataact ttggtcagac
    85861 gccgctgaat cagctctcac cggagcaatt aaaaaccatt ctcaccctgt tgcaggaagg
    85921 gaagatggtt attccgcaac cacagcagcg cgaggcgacc gaccgtcctt tattaccggc
    85981 ggagcacaat gcgctaaaac agctggtgac caaacttgcg gcggcaacgg gggaacccag
    86041 caaacagatc tggcaatcga tgctggaact ttccggggtg aaagatggcg agttaattcc
    86101 agcgaaactg tttaaccatc tggtgacctg gctacaggcg cgtcagacgc taagccagca
    86161 aaatacgccg acgctggaat cactacagat ggcgctaaaa caacctttag atgccagtga
    86221 actggcggcg ttatcggcat atatccagca aaaatatggc ctttctgcgc aatcatcgct
    86281 ttcttctgcc caggccgagg atattcttaa tcagctttat caacggcggg ttaaagggat
    86341 tgatccgcgt gatatgcaac cgctgcttaa tccttttcca ccgatgatgg acacgttgca
    86401 aaatatggca acgcgtcccg cgctgtggat actgttagtc gcgattatcc tgatgctggt
    86461 ctggctggtt cgttaacccc gacgaaatga cagtatcgtg acaataatac ccagcaccgc
    86521 agagatcgcc ccggcaagaa ataccgaaga gtaaccaaac gtggtcgcca gcattcccgc
    86581 cagcggcccg gagacgccga gggcgatatc ctgaaacgcg gcgtaaccgc ccagtgcggt
    86641 gccgcgaact tgtgagggga cgcgtttaac cacctccacg cccagcgcag gaaagataag
    86701 cgaacatccg gctccggtta acgccgcgcc cgctaatgcg acccatgcac ctggggcttg
    86761 ccagagcagc aacaagccca ccgtttctac aagcagagag acaatcgcca ctttcacgcc
    86821 gccaaaacgg tccggcatcc agccaaacat gacgcgcatc acgacaaatg cgccgccaaa
    86881 cgcggtaaga gtaaagcccg ccatcgccca tcctttgctg gcaaagcaga gcgaaacgaa
    86941 agtcccgata accgcaaaac caacgccttg tagtgccagc cctaaccctg gtttccagat
    87001 aagcccgaca acgctccaca gcgatggacg ttctcccgcc agggccggta ctttgcgcac
    87061 tgtgccgtta caggcccacg ccagtaaggg taataccatt gtggtgatcg ccagtgcggc
    87121 aaaaccgtaa tggctatgaa tcaacaggcc aagcggcgca ccaacagcga gggcaccgta
    87181 aatcgccatt ccgttccatg acatcacttt gccagagtgt tttggcccta cgatgcctaa
    87241 cccccaggtc agagcgcctg tcagtaactg gctttcacca aacccaagaa tcaaacgccc
    87301 gatgaccaac agggcaaatt tgaacggtgc ggagacaggc aaaatcgccg ccagcagcaa
    87361 cgcgccgcca gccagaccac aagctaacat cccctgaagc gccgaacgtt ttgcgccata
    87421 ttgatcggcc agtcgcccgg cgtaaccacg cgtcagcacc gtagccagaa actgaatccc
    87481 gacggcaatg ccgaccatgg tattgccata gcccagatca tgatgaacaa acagcgggat
    87541 aaccggcaac ggcaacccta cggtcatgta ggtgagaaaa accgcaaaag cgatgcggaa
    87601 gagcgaaaaa ctggcagaag atcgtgtttc ggtttggctt acagcagtca tgcattactc
    87661 cagaatgcag cgcaaggcga ggagtatccc cgtctcatct ctctggtttc agggttacgg
    87721 tgcgttggca ggatttaacg cgtacgtctt ttcagaagga aatcgacaaa gcgggaagtt
    87781 tgcctagaac tggtggcgat tgtcaatgat gtgaaaaagg gaaccatcag gttccctttt
    87841 gcgttagtgc cggaggccgc attggcgcgt aacgtcggat gcgacgctgg cgcgtcttat
    87901 ccgacctact ccggcctact gcgaattaat ctttcagctt gcgcattacc agcgtggcgt
    87961 tggtgccgcc gaagccgaag ctgttagaca taacagtggt cagttcgcga tcggtcgttt
    88021 cggtcacgat gttcagaccc gcagcctgct cgtccagctc ttcaatgttg atgctcgggg
    88081 cgataaagcc gtgttccagc atcagcagag agtagatagc ttcctgcaca cccgcagcgc
    88141 ccagtgagtg accggtcatg gctttggttg cagaaatcgc cgggctctta tcgccgaaca
    88201 cttcacggat agctgccagc tctttcacgt cgccaaccgg agtcgaagta ccgtgagagt
    88261 tcaggtaatc gattggagta tcaacgccat gcatcgccat ctgcatacaa cgtactgcgc
    88321 cttcgccaga cggagcaacc atgtctgcac catcagaggt tgcgccgtag ccaacgattt
    88381 cagcatagat gtaagcacca cgcgccagcg cgtgttccag ctcttcaacc actaccatac
    88441 cgccgccgcc agcgataacg aaaccgtcac ggtgagcgtc gtaagtacgg gaggcttttt
    88501 ccggggtgtc gttgtattta gtagacagcg cacccatcgc gtcgaattcg caggccattt
    88561 cccagcacag ctcttcgccg ccgccagcaa acacgatgtc ctgtttgccc agttggatct
    88621 gctctactgc gttaccgata cagtgtgcgg aagtcgcaca cgcggagctg atggagtagt
    88681 taacgccatg aattttaaac ggcgtggcga gacaggcaga aacgccggat gccatcgctt
    88741 tagtgaccac atacgggcca accgctttca ggccgcgcgg gccgcgcatt gcgtcagcgc
    88801 cgaacacctg gaaacgcggg gaaccgccgc cggaacctgc aatcaggcca acgcgcgggt
    88861 tattctggta agcttccgga gagaggcccg catcagcgat tgcctgctcc atagaaagga
    88921 atgcataaat ggatgcgtcg ctcataaagc gcacaacttt gcggtcaatg aggccagtgg
    88981 tatccagttt tatgttgccc cagacgtggc tacgcatgcc ggaatccttc agctcctgag
    89041 agaaagtgat ccctgaacgt ccttcacgca gagatgccag gacttcctgc tggttattac
    89101 cgatgctgga aacaatgccc aggccagtaa tcactgcacg tttcattcaa tacctctgta
    89161 agtcgcacat agagtaagtt tcgaatgcac aatagcgtac acttgtacgc cgaacaagtc
    89221 cgatcagcca tttaatagag aaatttgcgc agccttacac acatcgctaa gatcgagcca
    89281 ccgcctgtaa gacgagtaac ttacgtgaaa cactactcca tacaacctgc caacctcgaa
    89341 tttaatgctg agggtacacc tgtttcccga gattttgacg atgtctattt ttccaacgat
    89401 aacgggctgg aagagacgcg ttatgttttt ctgggaggta accaattaga ggtacgcttt
    89461 cctgagcatc cacatcctct gtttgtggta gcagagagcg gcttcggcac cggattaaac
    89521 ttcctgacgc tatggcaggc atttgatcag tttcgcgaag cgcatccgca agcgcaatta
    89581 caacgcttac atttcattag ttttgagaaa tttcccctca cccgtgcgga tttagcctta
    89641 gcgcatcaac actggccgga actggctccg tgggcagaac aacttcaggc gcagtggcca
    89701 atgcccttgc ccggttgcca tcgtttattg ctcgatgaag gccgcgtgac gctggattta
    89761 tggtttggcg atattaacga actgaccagc caactggacg attcgctaaa tcaaaaagta
    89821 gatgcctggt ttctggacgg ctttgcgcca gctaaaaacc cggatatgtg gacgcaaaat
    89881 ctgtttaacg ccatggcaag gttggcgcgt ccaggcggca cgctggcgac atttacgtct
    89941 gccggttttg tccgccgcgg tttgcaggaa gccggattca cgatgcaaaa acgtaagggc
    90001 tttgggcgca aacgggaaat gctttgtggg gtgatggaac agacattacc actcccctgc
    90061 tccacgccgt ggtttaatcg tacgggcagc agcaaacggg aagtagcgat tatcggcggt
    90121 ggtattgcca gcgcgttgtt gtcgctggcg ttattacggc gcggctggca ggtagcgctt
    90181 tattgcgcgg atgaagcccc cgcgctgggt gcttccggca atcgccaggg ggcgctgtat
    90241 ccgttattaa gcaaacacga tgaggcgcta aaccgctttt tctctaatgc gtttactttt
    90301 gctcgtcggt tttacgacca attacccgtt aaatttgatc atgactggtg cggcgtcacg
    90361 cagttaggct gggatgagaa aagccagcat aaaatcgcac agatgttgtc aatggattta
    90421 cccgcagaac tggctgtagc cgttgaggca aatgcggttg aacaaattac gggcgttgcg
    90481 acaaattgca gcggcattac ttatccgcaa ggtggttggc tgtgcccagc agaactgacc
    90541 cgtaatgtgc tggaactggc gcaacagcag ggtttgcaga tttattatca atatcagtta
    90601 cagaatttat cccgtaagga tgactgttgg ttgttgaatt ttgcaggaga tcagcaagca
    90661 acacacagcg tagtggtact ggcgaacggg catcaaatca gccgtttcag ccaaacgtcg
    90721 actctcccgg tgtattcggt tgccgggcag gtcagccata ttccgacaac gccggaactg
    90781 gcagagctga agcaggtgct gtgctatgac ggttatctca cgccacaaaa tccggcgaat
    90841 caacatcatt gtattggtgc cagttatcat cgcggcagcg aagatacggc gtacagtgag
    90901 gacgatcagc agcagaatcg ccagcggttg attgattgtt tcccgcaggc acagtgggca
    90961 aaagaggttg atgtcagtga taaagaggcg cgctgcggtg tgcgttgtgc cacccgcgat
    91021 catctgccaa tggtaggcaa tgttcccgat tatgaggcaa cactcgtgga atatgcgtcg
    91081 ttggcggagc aaaaagatga ggcggtaagc gcgccggttt ttgacgatct ctttatgttt
    91141 gcggctttag gttctcgcgg tttgtgttct gccccgctgt gtgccgagat tctggcggcg
    91201 cagatgagcg acgaaccgat tccgatggat gccagtacgc tggcggcgtt aaacccgaat
    91261 cggttatggg tgcggaaatt gttgaagggt aaagcggtta aggcgcggta atctgctctc
    91321 gcagtgtttg ccggatgcgg cgtgaacgcc ttatccaacc tacgtgtgac gtgtaggcct
    91381 gataagacgc gataagcgtc gcatcaggca tggtgctcca gacgccgcaa cttacagctg
    91441 tgacgcctgc tgaaacaggt tttcccacat atcggtcacc agcgcctggt cacgcggcga
    91501 caattcaccg gcaccaatgg ctttttccag actctggcta acggtcgtat gcaccgcctg
    91561 agcggagtgg tcatcaccac tttccagttc tgcgatggct aatgtcaggt ggccacgcaa
    91621 atacccactg gcaaacaact catcatcact ggcatggtca accatgccgt cgattaatgc
    91681 cagaatgcgt gattcaaact ccgcgatcat cttctttcct cattgtaaaa cgtggcgctg
    91741 gcttcagttg agcgcttccg gccacgggaa ctgttccgcc gtaagttccg gcgtgtgata
    91801 ataattttgt agtgctttaa tgaagcgtgc cgggcgttcc ggaatgccat ttgtcagata
    91861 atccatcacc tgcgcatgta cccgccgttg aaacaccaca cgatccggtt cgaaatcccc
    91921 ttccagattg tcgcaactga cgttaaacgg atatccagcc gccacgcaga ataaccagtc
    91981 gaacgcctgc ggcttcactt caacatcttc aaactggctt tgcgtctggg catcgcgtcc
    92041 gtccgggcaa taccagtagc cgaaatcaac cagctcacgg cgcgcttttc cggcaataca
    92101 ccagtgcgaa atctcatgaa ttgcgctggc ataaaagcca tgagcaaaga cgattcggtt
    92161 atacggtact tccgcatcag caggaagata gatcggttcg tcgtcgcctt taatcagacg
    92221 ggtattaaat tcatcggcaa agcagctatt aaaaatttca atcaactgct cgtagtggtg
    92281 tgtactgttc attagttcat ccccaaccag tggaggatct cctgtccgtg gctatcgtaa
    92341 agtaatttgg cactcatcac cgccgagacg ataacaatca tcgggcggat cagcttttgt
    92401 cctttgctca acaccaaccg tgaacccatg cgcgcgccga ggaactgccc caccagcatg
    92461 acaaagcccg tcgcccaaat cactttgccg ccgagaataa acagcagcag accgccaatg
    92521 tttgacgttg cgttgagtaa tttggcgtga gccgtggctt tggcgaggtt gaatccgcac
    92581 agcataacga aggccagcgc gtaaaacgat ccggcagccg ggccaaagaa tccgtcataa
    92641 aaaccgacgc agccaccggc aattaacgca aagggtaaac cgtacatccg gcgctggcga
    92701 tcttcttcac ccagctttgg catcagcaag aaatagaggc cgatacaaat caccagaatg
    92761 ggcaaaatct gccgtaagac atcagcctga acgtactgta ccaacagtgc gccgctcatt
    92821 gagccgacaa aggtcatggc aatattgagt ttctgatcgc ttaaactaac cactttgcgg
    92881 cgaataaagt agatagtggc ggaaatagag ccgccgcagg cttgcagttt attggttgcc
    92941 agcgcgttag cgggagacat tcccgcagcc atcaatgccg gaatggtgag taacccaccg
    93001 ccaccggcaa tcgagtcgat aaatcccgcc agcatggcga caaaaaagag aactcccagc
    93061 aacagcggag aaaccataaa caggctatta aacgtttcca ttagatcacg tgctcatcca
    93121 gtagcgcctg gcaggaaggc ggcaacggag gcggtgtctt cttctcaggc tttgttgttc
    93181 ccggttttgg aggttcaaac cagctttgca gttctgcccc gcaaccatcg cctgatggcg
    93241 gtaaaggttg atcttcacac tccagactat cggcagggca acgtaatcgt acatgcatat
    93301 gcgcgcgatg ctggaaccag ggtcgcactt tgcgcaacca gtcgcgatcg gtgcccgcat
    93361 caaggcaaag ttgttgttta atcgccggat taacaaaaat gcgcgtgacg tctttgtcct
    93421 gggcggcgag tttgatcaag ctgaaaattt ctggcttcca cagcgtggag acaacgtgtt
    93481 taccgtcgcg ggaaactaag tccagtgctt gcgggcgcaa gagctgcgcg gaggtccagc
    93541 gagttttcgg cagttgcaga aagatatcga catccagtcc ggtctggtgg ctggcatgac
    93601 cgccgttgaa acgcccacca gcgggcatcc ccatatcgcc aatcagcacc gtacccatgc
    93661 ccagattgct cacctggcta ctcagacgct ggataaacat caccagatcc gggtgaccga
    93721 aatagcgacg ctgatcggta cgcatgacct gataatgttc ggactgtatc ggcaacgtgt
    93781 cagcgccgac aatacagcca ttagaaaaac tgcctatcga ttgtgcgcta cccggcacag
    93841 gttgggttat tttttgccac ggcgtcgctg ccaggctagc gctactggca agcagagcca
    93901 gcagcgcaat cgcggttttg ttcatttttt accagcgtgg aatatcagtc ttcacatcgg
    93961 cattttgcgc ccgctgccgt aacaggtgat ccattaaaac gatcgccagc atcgcttcag
    94021 cgatcggcac tgcacggatc ccgacacagg gatcgtgacg gcctttggtg atcatctcaa
    94081 cttcttcgcc aaagcggtta atagtgcgcc ccggcacggt aatgctggag gtcggtttca
    94141 gcgccatatg agcaatgatt tgctgcccgc tgctgatacc gccgaaaatg ccgcccgcat
    94201 ggttgctctg gaaaccctct ttggtgattt cgtcgcggtt ctggctgccg cgcagcgcca
    94261 ctacgtcaaa accgtcgcca atttccacgc ctttcaccgc gttgatgctc atcagcgcat
    94321 gggcgatgtc ggcatccagg cggtcaaaga ccggctcgcc aagtccggcg gggacaccac
    94381 tggcaacaac ggtgactttc gcgccgatgg agtcgccctc ttttttcagc gcgcgcatca
    94441 gttcatctaa cgcgtcgatt ttgtcggggt ccgggcagaa gaacggattt tgttcaacca
    94501 gcgaccagtc tttgatttcc agcggaatgt cgcccatctg ggtcaggcag ccgcggattt
    94561 caataccaaa tttctcggcg agatattttt tggcaatcgc ccctgccgcc acgcgcatgg
    94621 cggtttcgcg ggcggaagaa cgtccaccgc cgcgataatc gcgcagaccg tatttttgtt
    94681 cgtaggtgta atcagcatgg cctggacgga agacgtcttt aattgcgctg taatcctgag
    94741 aacgctggtc ggtattttcg atcaacaagc caatgctggt gccagtagta acgccttcaa
    94801 aaacaccgga gagtattttg acctgatccg gctcgcggcg ctgggtggta tagcgcgatg
    94861 tcccagggcg acgacgatca aggtcatgtt gcaggtccgc ttccgtcagc ggaatgcctg
    94921 gcggaacacc atcgacgatg cagccgagcg ccagcccgtg cgattcgccg aaggtggtta
    94981 cgcgaaagag ttgtccaatt gtgtttccag ccatcacggc tccgttattg ttgtgtttgc
    95041 gtgtttactt aatctttata aatcgcgaaa tgttctcgtg cggcaagaag ctgctcttta
    95101 gtgagcataa acacaccatc gccgccgtta tcaaactcca gccaggtgaa cggaacatcg
    95161 ggatattgtt ccataagatg taccatgctg ttgcccactt cacaaatcaa cacgccatca
    95221 tcagcaaggt aatctgccgc gttaccgaga atgcgacgcg tcagtttcag gccgtcggtg
    95281 ccagatgcca ggcccagttc cggctcgtga cggtattcgt ttggcaggtc ggacatatct
    95341 tccgcgtcga catacggcgg gttagtgaca atcagatcgt attgcacttt cggcaaatca
    95401 cggaacagat cggaacgaat cggaatgacg ttgtggatca gaccgtgttc ttcgatgttc
    95461 tgttcggcaa ccgccagcgc gtcgggagag atatctaccg cgtcgacttc cgcttccggg
    95521 aaggcataag cacaggcaat ggcgatgcag ccgctaccgg tacacatatc taaaatatgc
    95581 tgcggttgct tgctgatgag tccggcaaat ttattgttga tcagttcacc aatcggcgag
    95641 cgcggcacca gcacgcgttc atcgacgtaa aattcatgac cgcagaacca ggctttgttg
    95701 gtcaggtaag ccaccggaat gcgctcgttg acgcggcgga tcacgcgttc aacaatacgg
    95761 tgtttttcgc tggaggtcag acgcgcggtg cgcatatctt ccgggatatc cagcggcagg
    95821 tagagcgatg gtaacaccag ctgtacggct tcatcccacg ggttatcggt gccgtgaccg
    95881 taccagatat ttgccgcgct gaagcggctt accgaccagc gcaacatgtc ctgaatggtt
    95941 tgcagctcat ttactgcttc atcaacgaaa attttatcca cgtattcctc cagggcatga
    96001 tcgcaataat ttcggcggct agtttgccat gaagatgacg ataaatcagc attcacgcgc
    96061 ggtgagtgag gaaaaatacg tttaaaacga tcgattgtgc tacgagtcgg gtaaactgta
    96121 ggaaaattag aaatagagac agataaatga aaaagaaaac aacacttagc gaggaggacc
    96181 aggctctgtt tcgccagttg atggcgggga ctcgcaagat taagcaggac acgattgtcc
    96241 accgaccgca gcgtaaaaaa atcagcgaag tgccggtgaa acgcttgatc caggagcagg
    96301 ctgatgccag ccattatttc tccgatgagt ttcagccgtt attaaatacc gaaggtccgg
    96361 tgaaatatgt tcgcccggat gtcagccatt ttgaggcgaa gaaactgcgc cgcggcgatt
    96421 attcaccgga attgtttttg gatttacacg gtctgacgca gctgcaggcc aagcaggaac
    96481 tgggcgcgtt gattgccgcc tgtcgccgcg aacatgtgtt ttgtgcctgc gtgatgcatg
    96541 ggcacgggaa gcatattttg aagcaacaaa caccgctgtg gctggcacaa catccgcatg
    96601 tgatggcatt tcaccaggca ccgaaagagt acggcggtga tgcggctttg ttggtgttga
    96661 ttgaagtgga agagtggttg ccgccggagt tgccctgaat gctggcagca aatgccggag
    96721 cagcgtaaac gccttatccg gcctacaaat atgtgataat tcaataaatt gcaattcact
    96781 tcgtaggcct gataagacgc atcagagcaa tactacgaac ttcagttcat tcaatacata
    96841 ctgaaaacga cacgtcgaat caatcggttg cccactactg gtattcacat tcaccacaat
    96901 atcgtgtagt actttatccg ttaccggatg acgagttggc gtaaccgtta ccgttgattt
    96961 atcacaaccg taagaggtaa aggttaacaa ttctgatacc gcgcggcagt atgctcctct
    97021 gccatcatta tcagaaaaat tactgccatc acagagtctg ccgccttcca tcaggtaggt
    97081 cgaaagcgtg gtggtcactg ttgaccccgt ggactgaatt cgtaacgcct tattatggct
    97141 gacataattt tcactccagg agatatcgta tgaagcgacc ggtatcgttg ggcatattgt
    97201 gttatgcact gtaggggccg tatagcttcc aaatccgggt aaatctgtat aggaataaaa
    97261 ggatgcgatc caagggcaac cataatcatc agatgcaacg gtaattttaa acgtcatata
    97321 tgcacaacga aaaattttcg tatctatgcc agggcaattt gagttattaa ttgtatcaaa
    97381 gtcagtcagt accaaagttg gagctgaacc cgacccagac caggaaataa cccgcaacgt
    97441 atcgccttta tacgcaaaag aatcatattt cccatatgca acctcctttg gcgttatcac
    97501 cgtaacattg aaaacactgc tcgctacagc accggttacc ttgtccatgg cataataaac
    97561 tctcacactg tcaaaactcg cactcataag tttatctcca gcataactct tagatgcgcc
    97621 gacaacactc agaacgagca gcattaggct cgcgaaaatt atggtccatt ttttcatttc
    97681 agaaatactc aaaggttgcc gtcactgtgg cactaaagtc tccggacgtt acatctcgcc
    97741 cgctctttgc ctgtaactag gtattaaaat ttaggctgtt atttccctga ttcagcggga
    97801 gcgtgacgcc tgttgtattg ttgattggca ccgatgtgcc tgccgcagtt tcaataccaa
    97861 tcccagcgcc ggatgctgat gacgaggagt caaaggccag aaagccgggt agagcactat
    97921 cttctgttcc tgtcaacgtt actttgacat taaacaatgt agaactatga caatctttca
    97981 attgaaacac caatggcact cgctctgact gcccaaggtt cattaaatcg ctggcggcaa
    98041 tggcaggaaa atgtacttct gccaactggc taccattaat cactggcgta cacgatttac
    98101 tgattaaatt gccggtaaag tgcaaattat ctcctgctat tgtggttcca ctgcccagca
    98161 acatcaggct actaataaaa aacaacgtac gctttttctt cattggtaat ctacctgtaa
    98221 cgtggcccag gcctcaaagt ccgcctctgg cagaatgaca ccactctttt ttactggcac
    98281 agccgttaac accggtaaat cggtttcatt caccacaagt ggcgtattga tcgtaaaact
    98341 ctgccctgcc tgtttgagct gaatgcctaa accagtgata ttgctgctta ctgcagcatt
    98401 atcaaaatcg cttggtgtgc cactccagct caaggtcatt tccattaatg ggtcgcgaac
    98461 cgccgtatcg caggtaatgg aatacggcac ggtttgctca taattgctgc cattaatgtc
    98521 atcaataagc acatcctgaa aagttatttc aatggtttga gcgttgctaa tcgtgcagtt
    98581 aggcggctgc actaaataac cgtgaaaaga gacgtttgag agtgccatcg agcaaaagcc
    98641 ccacagcaaa ataagcgaga tccgtttcat tggtaatcca ccacaattgt ggcactggcg
    98701 ttaaattcag ctgccgctaa cgttgtacca ctctgtttca ccggcacagc ttccagaacg
    98761 ggaactcccg aggaaaaatt aaatggcaac catgagccag aggttaagtc gagtattgtg
    98821 tgatcactac ttttctgaac ccgaatacca aacccaggaa tacccgtgct gataactgtt
    98881 tcaccgttaa tcacgaccgt tgtcgcctgc atctgtagac gcaggtcatc cattgccaga
    98941 ttgttacaaa caagcgagta gtcaatggga cgtttataat caaccctatt gatcttatta
    99001 atgaatacat taccaaactc aactgccgca ccatttaccg tacaaggagg aggggcatcg
    99061 acaagcaccc gtaaattcat acttactgac ttactcgctg caactgcaaa gccgctacca
    99121 acacaacata tcagtaaccc agttaccgcc cagacagaac aagatccttt catactggct
    99181 ctcccggcct cagcctttgc ggctctgctc ttcatcgacc tagcaactgt taccgttgca
    99241 agtgaaaaag agcggcatcc gagcgccata gtcgttgaca taggtcagta ccggcacgct
    99301 acccatcttc acattcagcg gtgcagaggt tttcggcgac atcactattg gcgagaaacc
    99361 ggctgccgga ttgccatttt tctgtgtgct ggcattactg aaaatgacgt aatacgacgt
    99421 cgggttattg acggtaaatt cctgaccgtt gcgggttaaa gtaaccttat attgataggg
    99481 gtttttcatg ctgacgttct ccagcgcctt cggtcgccag aacaacttaa tacgcgtttg
    99541 tagtgctatt tgcagcgtgt tggctttgtt tgattttggc gggatctcac gcacgttgaa
    99601 gtagaacagg ctttcacgat cggcaggcaa tttgttgata tccggcattc cctgcacttt
    99661 tacctgcccg ttcatcattg aatcgatgcg ttgcaccggt ggcagaacag ttaacggtga
    99721 actgatcttg ttaccctttt catcttcgat ccagctttgg gccagataag gcagtttcgg
    99781 atcgttattg cgcaacgtca cgctgattga tttgtcgctt tcattaaata tcaaacgggt
    99841 acgatctggc gttacagaag cctgggcaga caaactggca gcgctaagca gtaacgctag
    99901 cgtcgtagca cccaatttgg cagagcaaag taaatcagac atggatcatt ccttaatcac
    99961 gttattggtt agctgaaaca gaaacagggg gattggtggg tgtcacctgt tgcgtctgct
   100021 cctgaatgac aggctttatg tcatcaggaa caaccggcgc gacgttgcct ttatgctggc
   100081 atggcagtaa caagccgtta aacagatcgg caggcagcgg gtccggcagg ttgatatcgc
   100141 aatgcgcaac accactccag aacacactca tgtgttcgcc aggtttcacc ccagccagat
   100201 aaacattgcc gtcatcatcg acaaggccca ctgtctgctc gttatcattt tttacttctg
   100261 cgccaaacgg aggatggctg ccgtcttgta aacgcagcac cgccatcgct ttttgaccac
   100321 tgatgacggc aaatttgcgg tagccaatcg ccccttcagt tagcgtggct tgtaccaccg
   100381 actgggttgc ttcggcgttt tccggcagtt tgttgaggtc gatatacgcc tgattgcggt
   100441 aatagttatt gacgtcagaa acgacggctt taccaaacat attggtataa acagcagcac
   100501 cgttaccttc taccggaaca tcggcaacgc catcggcatc aatcaacaag cgtgtcccgc
   100561 ccatattctg ggtacggtga agtgcgccac cgtgggcagt aaacgttgcg ccgccctgta
   100621 acgacaagcc cgcagaggtg tattgccctt catggtagtt cgcactgagg tcaacctgcg
   100681 ccagcgaacc atcatggctg taatagccgt caacgctggt gtgtttgtca ctggtgccga
   100741 cgttcaactg atagtgagtc gcgtcatcga cacggctgaa ataaccgacc tgactgctgt
   100801 ccgtcccact gccatagtac cgttatagct aacggtactg ttgtcgcccc acggcatact
   100861 gagcgaaatg tacatgcctt tgtcggcctg gttgtcatac tcgtaacggt agccagtcat
   100921 cgagatgctg acattacgaa tactgcccat attgaaatag tgcgagagca tgatgttgta
   100981 gtttgtctgc tcctcgcgat cccagtaggt atggcgggta tagttgagat aaaccgaaac
   101041 acccgcatcg cggaagttct ggttataagt ggcggtgtac atctctttgt cgttgcccgt
   101101 gcgtaccatt ccgctgtcac tggcatccag atactcgctc atggtcataa agttctcttc
   101161 cgagaagcga tatccagcaa aagtaacgcg gctgttgagc tggtcaaagt ctttggaata
   101221 actcacacgg aaggagttac cgtccagcga acctttgcca taagcggtgt ctttatccag
   101281 tttggtgtgc gagtgagtaa catcaaacgc gaccgcgccg aatgtagaca aatcgcgacc
   101341 gacaccaagc gccgcagact gatagttttc atctcccagt gcgccgccat ataacgacca
   101401 gccgttagcg atcccccagg aagcttcagc accagaaaaa aattcaccct cgacatggtg
   101461 tccccactct tgcggacggc ccatcatgat cttatagcga acctgacctg gacgagtgag
   101521 gtatggcatc gaggcggtgc tgatgtcata ttcctgcacc tggccgttct gttcttcaat
   101581 gcgaatatgc aacgtaccgg agacagaatc accaagatcc tgaatacgaa acggtccagc
   101641 cggaacctgc gtttcgtaaa tcacacgccc catctggctg acggtcactt ttgctgtggt
   101701 gtgtgccacg ccggaaatgt ctggcgcgta gccgcgcaga ttgggaggca acatttgatc
   101761 gtcagtactg acactgccac caacatagtt aaaaccatca aaaatatcgg atctgaggta
   101821 atcctcgccc agcgccagtt tggcttttaa tgatggtaac gcccgcctgg catagtagcg
   101881 actccactcc catttttttt gagtttcatc gccgctgaat tcatcgtcat cattactgcg
   101941 agtatgttga tagttagtct gccagtcagc acgcatacgc cacggcccta ggttaacccc
   102001 aaccgtcccg ttgccgctga tctcgttact atcatcaccg ccattttctt cgtgccgggt
   102061 ttgtgcgttg atgctgtagt ccgcgacgat cccggagatg ccgtcatccc aacgtgaagg
   102121 cggatcccag tcgggataag tatattcgag gtaagcctgt ggcagtgaaa tgactaatgc
   102181 ggactggctt aaatcagcct taatttccat gccttccagt tgaccggatt tcaggcattt
   102241 agcatcgtgg ctccattgca gatttttcgc cacgtcttct tttaaaccaa actgcgctac
   102301 cagttccggt gtcagacaag catagctttt gctcgcgtca tcttcaccag cataccagta
   102361 aatatcgtac tcttccgcca atggctgttt atttagttga acctgtaaat tgtattttcc
   102421 gggctcaaca tatccctggc tggaaaaacg cttcagatca atttttgtgt cgccttttaa
   102481 ttccagaaaa cgggaatcaa actgaatgtc gtcttcagcc cagacactgc tataactccc
   102541 tgacattgcc agcgcaatgc accacggaag aatccgtaat cgaaaaagag aatggtcagg
   102601 catacacgtt tctatccgtt tttattcttg ttcggtcaaa atccatttaa atcccccata
   102661 gcagtgtcgc tatgggggta tgccttacag gtaagtaatc tggaaggtgg tatttgcttc
   102721 aaaattaccc agatctggcg catcagctgc gccgaccagc caggctttaa agttcagtgt
   102781 ctgcttcgct ttgcctttgt tggtagaagt gtctttcact attttctggt caatacccat
   102841 gccgcttttg taagaggtac ccagagagtc accgatcgcc aggctaacat tgttaaatgc
   102901 cgcaccggta tcggtattga aaatggtgta ataattgccg ctatttgcag aagaaacggt
   102961 accagtaaag gtagtggtca ttgtttcctg agtatcaaat acgcaatctt gcagacgaat
   103021 ctggaaactt ttcggcgtgg tggtgccgtt atttttcagt ttttcggcac cgatatcacc
   103081 catatcaact tccagtttgt gatcgtccgg aacgatagaa cacactgagt cttcaatggt
   103141 gccgtaaaaa cgtgcagtac cgttgttacc cgcagcgatt gttgccgtag aggtgaacac
   103201 gcccatcacc attgccgcag caatagctgt tttaacaaac ttactcatta tttgtcctta
   103261 aactataaat aattaaaaat ataaacattg caatacattg attcagtcaa tagccaatgt
   103321 ttatacccag ccattgaata cggcatgcga cccaaaaaga acaggtcgta gaaaattgta
   103381 ttcattggtg tggggccgca tagtgatgtt tccacacccc tgatccaatt taattttact
   103441 aaagagtaaa tgtagattta attaatatat tgacagaaga aaggttatct taaggacatc
   103501 cttaaaaaat acggaataag tatttagaaa aaatagaaag tatatcaata atgaatatca
   103561 acagcttaaa aaacaaacaa tgaacatata atgcgattgg catttatcca cttttgacaa
   103621 tataatatat aaacactata gccagaattt aatcaagttt caattacatc ctgcctgatt
   103681 attcaactct attttctcta ttatctgtat taataaatga cactgagtgt caaacagaca
   103741 ttattcattt caacatgtaa ctttgggcaa aatcaagata cgcgcagaag aataatttcc
   103801 gaatgaatgt agaaagtgtt ttatttatag ataaggagag tcttcgatag tagtgaggtt
   103861 gacagatatc cgctgcctaa tgagcaatta tgcccaatga atctacctca ttgggcataa
   103921 tttggaacac atcagatagc ttttgccatc ttcagattac acgggctcat ctgccagttg
   103981 aatgtaccgt ttccgctttc atcaagcgtt acgctggcaa tggccgaggt ggtaaacata
   104041 ggcggcgttt cgcccggaca taactcggca accagatacc cgactaacgg taagtgagag
   104101 atcaccagta ccgaagcaac accttcattg gtcaacgcct gcaaataggc actgaccaga
   104161 ccaacatcgc cgcagggcgt taactccggc agaacttctg cactggaagg caggttcaga
   104221 caatccccta cttcttccag tgtttgctcg gctcgcagga acgggctcac cagaacgcgt
   104281 tcgatttcca ctttttgacc tttcagccag ttcgccatca ggcgagattc gtcacaacca
   104341 ttagtggtca gaggacgaac cgaatcactg gcggcatcga gggctgcgtc gccgtgacgc
   104401 atgataaaaa cttgcatatt gcaccgcttt tgttaaccag attcgcccgt tttctttcat
   104461 ctgaaaccag agaaagaaaa cggtggccgg cattgtgcct tatccattca ccgaatgaaa
   104521 cgctgttttt tacctcaatg gcgtaagtat agtcaatatg cgtttacatt ttgaccaaca
   104581 ctccgccatt cagcgcggat tcatatagct ttgaccttct tattgcaggt cagttgcagt
   104641 tgttttccaa aaactttccc cacgcgcgcc catctcgacc aaacgctcgc aaggggtaaa
   104701 acgggaacca tactgcgtgg ccagtcgttg cattattgca accacttcgc ccgcgccgag
   104761 agaatcgata tagcggaacg gtccaccgag aaatggcgga aaaccaatgc caaataccgc
   104821 accaatatcc ccgtcacgca cgctacggat aacctgctca tcaacacaac gtactgcttc
   104881 attcagcatc aacatcacac accgttcagc aacctgcggt gcggagagtc gcccctgccc
   104941 ttgtgcgcca atgagcgggt aaatggcagg atcgacctgt tttttgcttt tacgcccttt
   105001 ctgaccataa agatagaaac cccgaccatt ttttctgcct ttgcgatcgt cgttcaaaat
   105061 tgaagaaaca acatttgcag gcgcgctaaa acgttctcca taagcggctt ccagtacagg
   105121 aataatttta gtcccggtgt cgattcctac ctcatccaaa agttggattg ggcctaccgg
   105181 aaaaccaaat ttcactagcg cggcatcaat gtgctctacc cgttcacctt cggtcagcat
   105241 gcggatagct tcattaatgt aaggcgctaa gatgcgattg acgtaaaacc cggctttgtc
   105301 acgcacgaca atgggcgttt tgccctgttt tttcgccagt tttaccgtag tggcgatggt
   105361 ttgcgccgat gtacccgcat gaggaataat ctctaccagc ggcatttttt ccaccggact
   105421 gaagaaatgc aggccaataa cttgctcagg tcgcgcggcg tgagcggcga tatcaccaat
   105481 cggtaaagat gacgtgttcg aagcaaaaat ggtatgagcg gcgcaatttt gctccacttc
   105541 cgccaccatc tgttgtttta attcgagatt ttcaaacacc gcttcaataa tcagatcgcg
   105601 atgggcaaag ccgcgatagt ccgtcgttcc ggagattaat gccagctgtt tgtcacgttc
   105661 gctggctttg agatgacgac ggcgaacttt gccctccagc tgatcccaac tgtacttcag
   105721 cgcatgattt atgccctgcg ggttgatatc tttaattctg accggaagcc ccgctttaca
   105781 agcagtgaca taagcaatac cgccgcccat caagccacca cctaaaattc ccacgctgtt
   105841 taatggcgca ggcggtgcat cactaccggg atctttcttc acgtccgtac tggcaaaaaa
   105901 gatactacgc aaagcttgcg attgcggcgt catcgccagt tcgccaaagg cgcgagcttc
   105961 agcgtcatag ccgctgctgg tgccctgcgc taatcccgtt tcaacaacct ccaggatgcg
   106021 ttctgtcgcc ggataattgc cttgagtttt gtgttctgtt ttcttgccga ccattttgaa
   106081 cagcagcgca cgacctaacg gccccgccag aatacgctcg cgtacaggta gagggcggga
   106141 agatgggcga tccttcttcg ccagctcaac agcggcttcc agcaaaatgg agtgcggaac
   106201 aacgtcatcc accagcccca gctttaatgc ctgtttcgcc cgaagttgtt ttccggtgag
   106261 aatcatctct aatgctgtac tgacgcctat cagacgcggt aaacgctggg tgccgcctga
   106321 accgggtaac aatccaagtt gtacttcagg caaaccgagc accgttttgg gatcgtcagt
   106381 acaaacgcga ccgtggcacg ccagcgccag ctccagccca ccgcccaggc aagcgccatg
   106441 aatagccgcg ataaccggaa tgggcaaagc atgaatctcc gccatcaact gttggccctg
   106501 ccgcgccagt gcttccgctt cttgcgccgt tttgcagttg ccaatcatgt tgatgtctgc
   106561 tccggcgata aaattgtccg gtttcgcgga gataaacacc acgccgcgca actctttgtt
   106621 ttcacggagt tgcttaataa tggcgcgcac ctgcgaggca aattccgcct tcagggtatt
   106681 cattttctca tccggtacgt cgatggtgat gatagcaatg ttgtccagac gaacattaag
   106741 agtaaacgct gatgccattt ccattattcc gcctccagaa ccattgccgc gccaagccca
   106801 ccggcagcac aggcggtaac taaaccaaat ctaccgccgc gacggcgaag ttcatgcaat
   106861 gtctgggtaa tcatccgcgc gccggtcgcc gcgaagggat gtccgtaagc aatcgaaccg
   106921 ccaagcacgt taaatttgct atcgtccact tcgccagtgg catgtgcacg ccccagtact
   106981 tcacgagcaa aacgttcact acccagcaac tgaatattcg ccagtgtctg agctgcaaag
   107041 gcttcgtgca tatcgatcaa tgtcagttcg ctcatcgtca aaccggcacg ttccagcgcc
   107101 agcggtgttg accaggctgg accgagcaac atgtcctgcc agacatcaat cgcagtaaat
   107161 gcgtagctgc gcagataccc cagcggcacc agccctaatt ctttcgcccg ggattcagtc
   107221 atcaggatca ccgctgccgc gccatcggtc agcggcgtac tgtttgccgc cgttaccgtt
   107281 ccgtgtttgc gatcaaacgc cgggcgcagc tttgcgtaat cggcaagcga ggaattaccg
   107341 cgaatattgt tgtcttcgac aagcggttgt ttataaggag ggataaaggc agtcatcacc
   107401 tcttctttga gttttccgtc tgaccatgcc tgagcggcac gctgatgcga acggtgcgct
   107461 aatgcatctt gctgttctcg ggtgatgccg taggttttcg ccatttgttc ggcggtgtcg
   107521 cccatccgca agccagtaga atattctgct accgcaggcg gtacgggcat taagtcgcgc
   107581 aaacgcaggc gagagaagag tttcaggcgc tggctcatgg ttcgagcttt gttgacatca
   107641 accagcacgt gcgccagttt tttactgacg ccaattggca atacggaaga ggaatctgcc
   107701 ccaccggcaa tccccgctcg gatagttccc gccatcaggc tttctgcgac gtttgcaacc
   107761 gcctggaaac tggtagcgca agcgcggctg acgctgtaag catcggtatg cacattcatt
   107821 ccggtaccga gaacaatttc acgcgcaatg ttgggggctt caggcatttg tacgacctga
   107881 ccaaagacca gctgttcaat cacttcagcg gggatctcgc tgcgtgccag cagttcgcct
   107941 accaccatct tccctaaatc aaccgcggga atgccatgaa aagccgtcgc ctggcgggca
   108001 aaaggcgtac gtaaaccgct aacaatggcg atacgatcgc cctggcgggt aaccagtggt
   108061 aaaacctgac ccataacact cccctgtaaa aaaataaata aagtggtctg acctgatcat
   108121 agtattaacc atttttttac atttagccaa gcggagaaaa aggaaagtgg gagctatgac
   108181 acagtgaaga cagaagaaag aaaagaaaac gcccctgcca gcaactgaca ggggcgtacg
   108241 tacccgagag gaattaacgc agacccagct ggaagatcag catttcagct tcgcaggcaa
   108301 aagtgaaatc gatatccagg cgcacaccgt cagactcttc agtgaaagtc ggggagattt
   108361 tgcacggttc ggattccacg ctacgggatt tttcagtcag cgccgccagc gtttgttctg
   108421 cttctgcgcg gtttgcaaac acgcggctgt aagacgcggt gcagtcggag ttgtccataa
   108481 tggtgccaac atccatacag cagcaaaccg gggtttcatc agcactacat ttactcatcg
   108541 ttgatttcct ctgtatgtgc acccaaggtg ccagataaac gttgtggata ttttacgctt
   108601 ccggaaagtg ctgctccagt tgttaattct gcaaaatcgg ataagtgacc gaaatcacac
   108661 ttaaaaatga tctaaaacaa aattcacccg aatccgcgag tgcgcaacct ccaaattttg
   108721 ccagctggat cgcgtttctt agatcatatt tgaaaaaaga tggaaacata cttgcaacat
   108781 tctagctggt ccgacctata ctctcgccac tggtctgatt tctaagatgt acctcagacc
   108841 ctacacttcg cgctcctgtt acagcacgta acatagtttg tataaaaata aatcattgag
   108901 gttatggtca tgagccagaa aaccctgttt acaaagtctg ctctcgcagt cgcagtggca
   108961 cttatctcca cccaggcctg gtcggcaggc tttcagttaa acgaattttc ttcctctggc
   109021 ctgggccggg cttattcagg ggaaggcgca attgccgatg atgcaggtaa cgttagccgt
   109081 aaccccgcat tgattaccat gtttgaccgc ccgacatttt ctgcgggtgc ggtttatatt
   109141 gacccggatg tgaatatcag cggaacgtct ccgtctggtc gtagcctgca agccgataac
   109201 atcgcgccta cggcatgggt tccgaacatg cactttgttg caccgattaa cgaccaattt
   109261 ggttggggcg cttctattac ctctaactat ggtctggcaa cagagtttaa tgatacttat
   109321 gcaggcggct ctgtcggggg tacaactgac cttgaaacca tgaacctgaa cttaagcggt
   109381 gcgtatcgct taaataatgc atggagcttt ggtcttggtt tcaacgccat ctacgctcgc
   109441 gcgaaaattg aacgtttcgc aggcgatctg gggcagttgg ttgctggtca gattatgcaa
   109501 tctcctgccg ggcaaactcc tcaagggcaa gcattggcag ctaccgccaa cggtatcgac
   109561 agtaatacca aaatcgctca tctgaacggt aaccagtggg gctttggctg gaacgccgga
   109621 atcctgtatg aactggataa aaataaccgc tatgcactga cctaccgttc tgaagtgaaa
   109681 attgacttca aaggtaacta cagcagcgat cttaatcgtg cgtttaataa ctacggtttg
   109741 ccaattccta ccgcgacagg tggcgcaacg caatcgggtt atctgacgct gaacctgcct
   109801 gaaatgtggg aagtgtcagg ttataaccgt gttgatccac agtgggcgat tcactatagc
   109861 ctggcttaca ccagctggag tcagttccag cagctgaaag cgacctcaac cagtggcgac
   109921 acgctgttcc agaaacatga aggctttaaa gatgcttacc gcatcgcgtt gggtaccact
   109981 tattactacg atgataactg gactttccgt accggtatcg cctttgatga cagcccagtt
   110041 ccggctcaga atcgttctat ctccattccg gaccaggacc gtttctggct gagtgcaggt
   110101 acgacttacg catttaataa agatgcttca gtcgacgttg gtgtttctta tatgcacggt
   110161 cagagcgtga aaattaacga aggcccatac cagttcgagt ctgaaggtaa agcctggctg
   110221 ttcggtacta actttaacta cgcgttctga taacgcgttc gcctggataa agtcacctgc
   110281 tatgcaggtg actttaactc ccccacttca ccgaaagtag tttcctcatt gcttgcctcc
   110341 tgagttttgc aaacagcctg atggcagctt gccctttcaa atcagtaagt ggtatccaca
   110401 gacaaacctg aagggaaatg catattttca agcgttctgc tcgtccttct caaagagttt
   110461 acttttctgc atttccagga tactcaaccc ccctggctat tgtgcgctta tacactcaaa
   110521 ttaaagatag gttctaaata aatgagcgtt ttttgatagt ctatttcatt aggtaatata
   110581 tatttgtaac aaatcaatca acatagaata aaatcatgct accatctatt tcaatcaaca
   110641 ataccagcgc agcttaccca gaatccatca atgaaaataa caatgatgaa attaatggat
   110701 tagtacaaga gttcaaaaac ctttttaatg gtaaggaagg aataagcacc tgtattaaac
   110761 atctacttga gcttataaaa aacgccatac gagtaaacga cgatccttat agatttaata
   110821 ttaataattc ctcagttact tatattgata ttggctccaa tgatacagac catattacta
   110881 ttggtatcga caaccaagaa ccaatagaat tacctgcgaa ctataaagac aaagaactcg
   110941 tccgtactat cattaatgac aacatagttg agaagactca tgatatcaat aacaaggaaa
   111001 tgatcttcag cgcattaaaa gaaatatatg atggagatcc tggttttatc ttcgataaga
   111061 tatcacacaa actcagacat acggtaacgg aatttgatga gagcgggaaa agcgaaccaa
   111121 cggacttatt tacctggtac ggtaaagata aaaaaggcga ctctctcgct attgtaatta
   111181 aaaataaaaa cggaaatgat tacttatctc tcggttacta cgatcaggac gactaccaca
   111241 ttcaaagagg aattcgtatt aatggtgata gtctcaccca atattgtagt gaaaacgcca
   111301 ggagtgcttc agcgtggttt gaaagcagta aagctatcat ggcagaatca tttgcaactg
   111361 gttccgatca tcaggttgta aacgagctca acggggaaag actgagagaa cctaacgaag
   111421 tttttaaacg tttaggtcga gcaataagat ataattttca agtggacgat gcaaaattta
   111481 gacgcgataa tgtaaaagaa ataatttcta ctttattcgc taacaaagtt gacgttgacc
   111541 atcctgaaaa taaatacaaa gactttaagg atctcgaaga taaagttgaa aaaagacttc
   111601 aaaattgcca ggcgaaatat caaaatgaaa ttaatcaact atctgcacta ggtgttaatt
   111661 ttgatgatat ttaaaattat ttttccagtt ttttgatttt tcaacgcctg aatatcggaa
   111721 atagtcagtg acaaac
//
LOCUS       DAJTYF010000003        86253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTYF010000003 DAJTYF010000000
VERSION     DAJTYF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 86253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.003"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..1593)
                     /locus_tag="OO590_000241"
     CDS             complement(<1..1593)
                     /locus_tag="OO590_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6635746.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDKNEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAE"
     gene            complement(1577..1933)
                     /locus_tag="OO590_000242"
     CDS             complement(1577..1933)
                     /locus_tag="OO590_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6635747.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2056..2238)
                     /locus_tag="OO590_000243"
     CDS             complement(2056..2238)
                     /locus_tag="OO590_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635748.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2222..2662)
                     /locus_tag="OO590_000244"
     CDS             complement(2222..2662)
                     /locus_tag="OO590_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6635749.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2662..2958)
                     /locus_tag="OO590_000245"
     CDS             complement(2662..2958)
                     /locus_tag="OO590_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6635750.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2955..3293)
                     /locus_tag="OO590_000246"
     CDS             complement(2955..3293)
                     /locus_tag="OO590_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6635751.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3290..4501)
                     /locus_tag="OO590_000247"
     CDS             complement(3290..4501)
                     /locus_tag="OO590_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6635752.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4503..5075)
                     /locus_tag="OO590_000248"
     CDS             complement(4503..5075)
                     /locus_tag="OO590_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6635753.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5115..6272)
                     /locus_tag="OO590_000249"
     CDS             complement(5115..6272)
                     /locus_tag="OO590_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR6635754.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6572..6796)
                     /locus_tag="OO590_000250"
     CDS             complement(6572..6796)
                     /locus_tag="OO590_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635755.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(6938..7099)
                     /locus_tag="OO590_000251"
     CDS             complement(6938..7099)
                     /locus_tag="OO590_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6635756.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(7109..7414)
                     /locus_tag="OO590_000252"
     CDS             complement(7109..7414)
                     /locus_tag="OO590_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635757.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7411..7689)
                     /locus_tag="OO590_000253"
     CDS             complement(7411..7689)
                     /locus_tag="OO590_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635758.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7969..9717)
                     /locus_tag="OO590_000254"
     CDS             complement(7969..9717)
                     /locus_tag="OO590_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6635759.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9714..10013)
                     /locus_tag="OO590_000255"
     CDS             complement(9714..10013)
                     /locus_tag="OO590_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6635760.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(10019..10252)
                     /locus_tag="OO590_000256"
     CDS             complement(10019..10252)
                     /locus_tag="OO590_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635761.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(10254..10474)
                     /locus_tag="OO590_000257"
                     /pseudo
     CDS             complement(10254..10474)
                     /locus_tag="OO590_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10467..10688)
                     /locus_tag="OO590_000258"
     CDS             complement(10467..10688)
                     /locus_tag="OO590_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635762.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10678..10878)
                     /locus_tag="OO590_000259"
     CDS             complement(10678..10878)
                     /locus_tag="OO590_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6635763.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10887..11633)
                     /locus_tag="OO590_000260"
     CDS             complement(10887..11633)
                     /locus_tag="OO590_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635764.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11691..11879)
                     /locus_tag="OO590_000261"
     CDS             complement(11691..11879)
                     /locus_tag="OO590_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6635765.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(12274..13503)
                     /locus_tag="OO590_000262"
     CDS             complement(12274..13503)
                     /locus_tag="OO590_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6635766.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13752..14873
                     /gene="roxA"
                     /locus_tag="OO590_000263"
                     /gene_synonym="ycfD"
     CDS             13752..14873
                     /gene="roxA"
                     /locus_tag="OO590_000263"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6635767.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14922..16148)
                     /gene="pepT"
                     /locus_tag="OO590_000264"
     CDS             complement(14922..16148)
                     /gene="pepT"
                     /locus_tag="OO590_000264"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6635768.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16398..17534
                     /gene="potA"
                     /locus_tag="OO590_000265"
     CDS             16398..17534
                     /gene="potA"
                     /locus_tag="OO590_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6635769.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17518..18375
                     /gene="potB"
                     /locus_tag="OO590_000266"
     CDS             17518..18375
                     /gene="potB"
                     /locus_tag="OO590_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6635770.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            18372..19166
                     /gene="potC"
                     /locus_tag="OO590_000267"
     CDS             18372..19166
                     /gene="potC"
                     /locus_tag="OO590_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6635771.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            19163..20209
                     /gene="potD"
                     /locus_tag="OO590_000268"
     CDS             19163..20209
                     /gene="potD"
                     /locus_tag="OO590_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6635772.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(20365..21186)
                     /gene="cobB"
                     /locus_tag="OO590_000269"
     CDS             complement(20365..21186)
                     /gene="cobB"
                     /locus_tag="OO590_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6635773.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCHFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(21202..22113)
                     /gene="nagK"
                     /locus_tag="OO590_000270"
     CDS             complement(21202..22113)
                     /gene="nagK"
                     /locus_tag="OO590_000270"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6635774.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(22142..23386)
                     /gene="lolE"
                     /locus_tag="OO590_000271"
     CDS             complement(22142..23386)
                     /gene="lolE"
                     /locus_tag="OO590_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6635775.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23386..24087)
                     /gene="lolD"
                     /locus_tag="OO590_000272"
     CDS             complement(23386..24087)
                     /gene="lolD"
                     /locus_tag="OO590_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6635776.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(24080..25279)
                     /gene="lolC"
                     /locus_tag="OO590_000273"
     CDS             complement(24080..25279)
                     /gene="lolC"
                     /locus_tag="OO590_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6635777.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25541..26587
                     /locus_tag="OO590_000274"
     CDS             25541..26587
                     /locus_tag="OO590_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6635778.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26715..30161
                     /gene="mfd"
                     /locus_tag="OO590_000275"
     CDS             26715..30161
                     /gene="mfd"
                     /locus_tag="OO590_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6635779.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            30304..31266
                     /gene="ldtC"
                     /locus_tag="OO590_000276"
                     /pseudo
     CDS             30304..31266
                     /gene="ldtC"
                     /locus_tag="OO590_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(31349..31606)
                     /gene="bhsA"
                     /locus_tag="OO590_000277"
     CDS             complement(31349..31606)
                     /gene="bhsA"
                     /locus_tag="OO590_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR6635780.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31847..32479
                     /gene="comR"
                     /locus_tag="OO590_000278"
     CDS             31847..32479
                     /gene="comR"
                     /locus_tag="OO590_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6635781.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32541..33080)
                     /locus_tag="OO590_000279"
     CDS             complement(32541..33080)
                     /locus_tag="OO590_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6635782.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(33307..34611)
                     /gene="ndh"
                     /locus_tag="OO590_000280"
     CDS             complement(33307..34611)
                     /gene="ndh"
                     /locus_tag="OO590_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6635783.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(35012..35554)
                     /locus_tag="OO590_000281"
     CDS             complement(35012..35554)
                     /locus_tag="OO590_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6635784.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35577..36602)
                     /gene="nagZ"
                     /locus_tag="OO590_000282"
     CDS             complement(35577..36602)
                     /gene="nagZ"
                     /locus_tag="OO590_000282"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6635785.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36613..37437)
                     /gene="thiK"
                     /locus_tag="OO590_000283"
     CDS             complement(36613..37437)
                     /gene="thiK"
                     /locus_tag="OO590_000283"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6635786.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37418..38059)
                     /gene="lpoB"
                     /locus_tag="OO590_000284"
     CDS             complement(37418..38059)
                     /gene="lpoB"
                     /locus_tag="OO590_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6635787.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(38074..38451)
                     /locus_tag="OO590_000285"
     CDS             complement(38074..38451)
                     /locus_tag="OO590_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6635788.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38454..38813)
                     /gene="hinT"
                     /locus_tag="OO590_000286"
     CDS             complement(38454..38813)
                     /gene="hinT"
                     /locus_tag="OO590_000286"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6635789.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            39147..39971
                     /locus_tag="OO590_000287"
     CDS             39147..39971
                     /locus_tag="OO590_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6635790.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPT"
     gene            39975..41336
                     /gene="fhuE"
                     /locus_tag="OO590_000288"
                     /pseudo
     CDS             39975..41336
                     /gene="fhuE"
                     /locus_tag="OO590_000288"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41396..42829)
                     /gene="ptsG"
                     /locus_tag="OO590_000289"
     CDS             complement(41396..42829)
                     /gene="ptsG"
                     /locus_tag="OO590_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6635791.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(43124..43921)
                     /locus_tag="OO590_000290"
     CDS             complement(43124..43921)
                     /locus_tag="OO590_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6635792.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43932..44936)
                     /gene="holB"
                     /locus_tag="OO590_000291"
     CDS             complement(43932..44936)
                     /gene="holB"
                     /locus_tag="OO590_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6635793.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAALVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44933..45574)
                     /locus_tag="OO590_000292"
     CDS             complement(44933..45574)
                     /locus_tag="OO590_000292"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6635794.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45564..46586)
                     /gene="yceG"
                     /locus_tag="OO590_000293"
     CDS             complement(45564..46586)
                     /gene="yceG"
                     /locus_tag="OO590_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6635795.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46589..47398)
                     /gene="pabC"
                     /locus_tag="OO590_000294"
     CDS             complement(46589..47398)
                     /gene="pabC"
                     /locus_tag="OO590_000294"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6635796.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47518..48759)
                     /gene="fabF"
                     /locus_tag="OO590_000295"
     CDS             complement(47518..48759)
                     /gene="fabF"
                     /locus_tag="OO590_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6635797.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48846..49082)
                     /gene="acpP"
                     /locus_tag="OO590_000296"
     CDS             complement(48846..49082)
                     /gene="acpP"
                     /locus_tag="OO590_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6635798.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(49293..50027)
                     /gene="fabG"
                     /locus_tag="OO590_000297"
     CDS             complement(49293..50027)
                     /gene="fabG"
                     /locus_tag="OO590_000297"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6635799.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGSQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(50040..50969)
                     /gene="fabD"
                     /locus_tag="OO590_000298"
     CDS             complement(50040..50969)
                     /gene="fabD"
                     /locus_tag="OO590_000298"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6635800.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50985..51938)
                     /gene="fabH"
                     /locus_tag="OO590_000299"
     CDS             complement(50985..51938)
                     /gene="fabH"
                     /locus_tag="OO590_000299"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6635801.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(52006..53076)
                     /gene="plsX"
                     /locus_tag="OO590_000300"
     CDS             complement(52006..53076)
                     /gene="plsX"
                     /locus_tag="OO590_000300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6635802.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(53157..53330)
                     /gene="rpmF"
                     /locus_tag="OO590_000301"
     CDS             complement(53157..53330)
                     /gene="rpmF"
                     /locus_tag="OO590_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6635803.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(53382..53903)
                     /gene="yceD"
                     /locus_tag="OO590_000302"
     CDS             complement(53382..53903)
                     /gene="yceD"
                     /locus_tag="OO590_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6635804.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            54102..54686
                     /gene="yceF"
                     /locus_tag="OO590_000303"
     CDS             54102..54686
                     /gene="yceF"
                     /locus_tag="OO590_000303"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6635805.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54798..55757)
                     /gene="rluC"
                     /locus_tag="OO590_000304"
     CDS             complement(54798..55757)
                     /gene="rluC"
                     /locus_tag="OO590_000304"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6635806.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            56330..59515
                     /gene="rne"
                     /locus_tag="OO590_000305"
     CDS             56330..59515
                     /gene="rne"
                     /locus_tag="OO590_000305"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6635807.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59712..60665)
                     /gene="flgL"
                     /locus_tag="OO590_000306"
     CDS             complement(59712..60665)
                     /gene="flgL"
                     /locus_tag="OO590_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR6635808.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60677..62319)
                     /gene="flgK"
                     /locus_tag="OO590_000307"
                     /pseudo
     CDS             complement(60677..62319)
                     /gene="flgK"
                     /locus_tag="OO590_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62385..63326)
                     /gene="flgJ"
                     /locus_tag="OO590_000308"
     CDS             complement(62385..63326)
                     /gene="flgJ"
                     /locus_tag="OO590_000308"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6635809.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(63326..64423)
                     /gene="flgI"
                     /locus_tag="OO590_000309"
     CDS             complement(63326..64423)
                     /gene="flgI"
                     /locus_tag="OO590_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6635810.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64435..65133)
                     /gene="flgH"
                     /locus_tag="OO590_000310"
     CDS             complement(64435..65133)
                     /gene="flgH"
                     /locus_tag="OO590_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6635811.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(65186..65968)
                     /gene="flgG"
                     /locus_tag="OO590_000311"
     CDS             complement(65186..65968)
                     /gene="flgG"
                     /locus_tag="OO590_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6635812.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(66106..66857)
                     /gene="flgF"
                     /locus_tag="OO590_000312"
                     /pseudo
     CDS             complement(66106..66857)
                     /gene="flgF"
                     /locus_tag="OO590_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(66877..68076)
                     /gene="flgE"
                     /locus_tag="OO590_000313"
     CDS             complement(66877..68076)
                     /gene="flgE"
                     /locus_tag="OO590_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6635813.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTCAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIVATT
                     QNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQ
                     SSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQIL
                     NTRVNLR"
     gene            complement(68101..68796)
                     /gene="flgD"
                     /locus_tag="OO590_000314"
                     /pseudo
     CDS             complement(68101..68796)
                     /gene="flgD"
                     /locus_tag="OO590_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(68808..69211)
                     /gene="flgC"
                     /locus_tag="OO590_000315"
                     /pseudo
     CDS             complement(68808..69211)
                     /gene="flgC"
                     /locus_tag="OO590_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(69215..69388)
                     /locus_tag="OO590_000316"
     CDS             complement(69215..69388)
                     /locus_tag="OO590_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HCR6635814.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69543..70202
                     /gene="flgA"
                     /locus_tag="OO590_000317"
     CDS             69543..70202
                     /gene="flgA"
                     /locus_tag="OO590_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6635815.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            70278..70554
                     /gene="flgM"
                     /locus_tag="OO590_000318"
                     /pseudo
     CDS             70278..70554
                     /gene="flgM"
                     /locus_tag="OO590_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
     gene            70559..70975
                     /gene="flgN"
                     /locus_tag="OO590_000319"
     CDS             70559..70975
                     /gene="flgN"
                     /locus_tag="OO590_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6635816.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(71015..72550)
                     /gene="murJ"
                     /locus_tag="OO590_000320"
     CDS             complement(71015..72550)
                     /gene="murJ"
                     /locus_tag="OO590_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6635817.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSHLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72717..73583)
                     /locus_tag="OO590_000321"
     CDS             complement(72717..73583)
                     /locus_tag="OO590_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6635818.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(73585..74232)
                     /locus_tag="OO590_000322"
     CDS             complement(73585..74232)
                     /locus_tag="OO590_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6635819.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(74243..74827)
                     /gene="rimJ"
                     /locus_tag="OO590_000323"
     CDS             complement(74243..74827)
                     /gene="rimJ"
                     /locus_tag="OO590_000323"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6635820.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            75063..76271
                     /gene="mdtH"
                     /locus_tag="OO590_000324"
     CDS             75063..76271
                     /gene="mdtH"
                     /locus_tag="OO590_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6635821.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEYR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            76335..76982
                     /gene="grxB"
                     /locus_tag="OO590_000325"
     CDS             76335..76982
                     /gene="grxB"
                     /locus_tag="OO590_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6635822.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYHDNMAKQTQINLLSSMAI"
     gene            77116..77676
                     /locus_tag="OO590_000326"
     CDS             77116..77676
                     /locus_tag="OO590_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6635823.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77782..78828
                     /gene="pyrC"
                     /locus_tag="OO590_000327"
     CDS             77782..78828
                     /gene="pyrC"
                     /locus_tag="OO590_000327"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6635824.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78902..79147
                     /gene="dinI"
                     /locus_tag="OO590_000328"
     CDS             78902..79147
                     /gene="dinI"
                     /locus_tag="OO590_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6635825.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            79437..79691
                     /gene="bssS"
                     /locus_tag="OO590_000329"
     CDS             79437..79691
                     /gene="bssS"
                     /locus_tag="OO590_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6635826.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            79806..80924
                     /gene="solA"
                     /locus_tag="OO590_000330"
     CDS             79806..80924
                     /gene="solA"
                     /locus_tag="OO590_000330"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6635827.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            80972..81085
                     /locus_tag="OO590_000331"
     CDS             80972..81085
                     /locus_tag="OO590_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6635828.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            81346..81912
                     /locus_tag="OO590_000332"
     CDS             81346..81912
                     /locus_tag="OO590_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6635829.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            81916..82491
                     /locus_tag="OO590_000333"
     CDS             81916..82491
                     /locus_tag="OO590_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6635830.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(82533..83585)
                     /locus_tag="OO590_000334"
     CDS             complement(82533..83585)
                     /locus_tag="OO590_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6635831.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            83810..84730
                     /gene="lpxL"
                     /locus_tag="OO590_000335"
     CDS             83810..84730
                     /gene="lpxL"
                     /locus_tag="OO590_000335"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6635832.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            84902..85099
                     /locus_tag="OO590_000336"
     CDS             84902..85099
                     /locus_tag="OO590_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6635833.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            85123..>86253
                     /locus_tag="OO590_000337"
     CDS             85123..>86253
                     /locus_tag="OO590_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6635834.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLALCDRFETENTDTIDSLKLQREDL"
BASE COUNT        20698 a        22924 c        21234 g        21397 t
ORIGIN      
        1 ctctgcctgg aatgtaccaa acgccatcag cgccgccacg ctcgggtcta tcttgttcgg
       61 tgattttttc ttgttcggct tgatattggc gttcgcgtca ctctgcatca cgacgttact
      121 catcgaccag gacaacaccg gatcgccacg atgcacaatc accctgcggt taacaaaaac
      181 ttcgaacgat ttcgccgccg gactgaatct gaggtaggtt tgcgggaacg gctccacctc
      241 aaaaccagcc ccctgtaatt gcgtcctgag atgcgtggcg ttccacgtat caaagcccac
      301 cagcctgata ttaaatttct ccgcatcctg catgatgtca tctctgatcc ggtcgtaatc
      361 aatgcagtca cctggcgttg tgcgtatcca gcccgcttta gcccactggc gatagacagc
      421 gcggttttta ttggcggggt tctgtagctg gaactccggc agataatgac gggaaatcag
      481 cataatggtt ttaccgaccg gaaaggcata gcatacgctg gaaatatcgc tggttgatga
      541 taagtccagc cccgcgtaac actcctgacc gtgtaaatct tcctccgtga acgttccggc
      601 acactcagcc catgcaccat tacccatcca cggggtagtc ccctgacacc agatattaaa
      661 tcgctttgtc atcatctcca cccactgcga cggaataccc cgcgctttct ggatggttga
      721 ggccagtttt tcacgatcca cggaaacatc aatattaggg ttcgccttta tccacatcgc
      781 cggatcatca acctcgtttt tgtcgtccag ttcgtaaatc agtacaaaaa ttgaatcgtt
      841 gacctcttcg ccgtccagga tctggcagca ataatcatag tgctgtttac aggctgaaac
      901 gacgttactg cccgatgtgg taatggcaaa taacagccct tcgggacgtg cgcccatccc
      961 cagctcaagc gcggaataaa cgccgttatc ggggtgtagg tggtattcgt ccacgatgga
     1021 aagactgggg tttgtcccct cgatggttgc cgcttttgct gccagcggct ttaacaggct
     1081 gttgcttttc gggtgtatca ccttatgcgc ctgaatattc acccgcctgc gtaacggtcg
     1141 ggataaaagg cacatctgac gcgcatcatc aaacacgatc cgcgcctggt cacggctcac
     1201 cgctgcggtg tagatatcct gctgaccgtt ctccataatc agaaaccagt tagccagaat
     1261 cgcggcggtc gtggatttcg catttttgcg cggcacttcg ataaaggcgc tcgtgtattt
     1321 gcgccgtccg gtggccttaa ccttaaagcc gaggatgcac gcaaaggcga actgctgcca
     1381 cggctccagc tcaatgggtc tgccacgcat cggtcctttt acgtgcgggc acaccctgga
     1441 aaaggcaata aaccgctcca caacctcacg atcgaacatg taaagggggc ttttaaggtc
     1501 cgaaaagtac cgtttaacag cctgttttaa ccgtttacag gccggaattt tgcccgtttt
     1561 tacgtcttct gcgtacttat tccaggcggt caagctcgtc ctcttcttct gtttccggtg
     1621 gatttttacg gcggcttatc gggtcaaaac cgagcaagga ggccattttt atcatcactc
     1681 tttcagcgtc ggattttgcg cttaatgcgg ggtttctgct ctcgccgccc tgactgttaa
     1741 caatgctgaa cccgcgcgcc gcaaggtctg caacggcttt ccggtaaatg gagtagttga
     1801 cgcaatacag ttccagattg ctccagtcgg cgggggtcag gtctccccgt tccgcaagct
     1861 gccgcgattt ttcccgccac tgcttcacgg cgatatcatc caggtaggcg ggggctttcg
     1921 gtggtcttgc catgcttatt tttcgtcaga ttatttttca aaaaattccc gtgcataaaa
     1981 atttggggag gcgttcggtg cccggcgggg tcgggtttgt cctgaaaacg ccccccaccc
     2041 cgtcatacag cctcatcagc gattgcggaa acattccata acctcgcggt cacggtcggt
     2101 taatcgcttc gctgtgatgc gttctgcgcg tcctgacgct ttatctttat gccctgtttc
     2161 ctgtgtcttc catgcgtcac gctgccttat aagtccacgg ataaggcggt tttgttcccg
     2221 ctcattcatc agcgtcatac atccagttat tgcggttagc agcccgttct tcctcttcac
     2281 gaaatccacc tgcggcacgc ttgctttttg ttgccggatc aagccatttc gttttctggt
     2341 tatgacacgc ctggcacaat ggttgatggt tccattcggg ccagaagaga acatcatcac
     2401 cgccattaat cgggataatg tgatccacca ccacggcggg cgtatatatc cccttctcaa
     2461 ggcatcgcac gcataacggg tttttactca gatacatggc gcggtatttg tcccactgtc
     2521 tggaatatcc acgcgcgcgg cggtgtcctc gtctggcatc ctctgcacgc catgccgccc
     2581 gcctgtgctc ctcacacttg ccggatttaa cgcgcctgtt acagcctggt tctgtgcatc
     2641 gtcttaatgg ttgccacggc atcagtacac ccccacatca cggtaagccg tccagagtgc
     2701 gcctatcgtc atgggtacgc gtgtttttgc gttatccgcg acaatctggc gattttcata
     2761 caggtgagcg ataaacatca tgcagccaat ctttatggct ggcgtgaacg ccagcccgtc
     2821 atcaaagcgc ctgcctatgt gtatctggca cgcctcaagc gatgcggcaa tgtatccgct
     2881 gattaactgg tcttcatcgt cgccatcgat gcggcaatgg agtttcactt cttccagggt
     2941 gataagttct tctgtcattt ttccgcgccc tcacgacaaa gaatttcaag gcgtgtcctg
     3001 gcggcatccg gcagcggctg cccgatgata ttcagtacac gccccgccag cggccccgta
     3061 ctgaccttta tccggctggt ggcgttgatg tccttccggt accgtatcca gatccttacg
     3121 gtaccaatcg ccagctctgc gcccgatgaa atggcctcct tgctgctgac catgttcacg
     3181 cttgcccaga gtgtgtgacc gtcctcccac gtttcgagta tttcgccggt tatatccctg
     3241 gtctgtttca gggtctgaat cgttatcctg tcacgcagtc gccctatgtt cattccggtt
     3301 tttctccctc gctgattttt acttcctgtt tccatgcctg gctgaactca tcaccaccat
     3361 cacgcggtga cagcccttca cgttcgcggg cttcgttcgg acacataacg ccggatttta
     3421 tgccgcgctc ataggtggca aagcgttcgc caggtgtggc ccgtaacagg tccgcgctgt
     3481 caaactccac ctgataccgg ataccaggta caggcgaggc caccagcaag gcatttttta
     3541 tctgctgctc aaagttcgcc agccacgggc gcatcgtcat ggtgagaaat gcgcggcttg
     3601 cctcgctgaa gttgctgtag gtgctgttgc tgtattcctg caaaaaaatc ggcgagacgt
     3661 tgaacattcg ggcgatgtct tcaatggtga agcgacggga ggccagccat tcggcatcct
     3721 gattactcat gcccagctgc tgatagctca tccccccttc aaggatgggc gtttttccgg
     3781 cgtttctggc ccctttgtag cgttccagtg cggctaatgc ctgtttgccc ttcacgccgt
     3841 ccagccattc gcctgacgtg ataacccctg ccgccatcat gccatcacgc atcacgctcg
     3901 cgccgtggcg ttgttgggcc agcccaagcc ccagcgattc gcggcagatg gttacaggtg
     3961 agcgccccat aaaaccgtca tccgtggagt aacgaaggtg gagaacctcc cacggtaaat
     4021 agttgcgggt gtttccggtg taggcgtcag tgatgcagta gcgccagtta tgatcacctg
     4081 tctgctccac gttcaccgac tgcggcgggt aaggatgtaa agccgccgga aaaccatcac
     4141 gcccccactg aataaccgca taagcattac cacttaacag gcagtggcgg atcatcattc
     4201 gcttaaactg gtagggtgtc tgccacgcgt tcgggcgctc gttgaggatg tgatcgaccg
     4261 gatgagaatc aagccattcg cgggcctcct tccccttctc attgcgtacc aggtacaggt
     4321 aacacggcat ggtagccacc gcctcagaga tgaccgtgac ggcgttcatg acggcggaga
     4381 gtgattccgc tgtcccagat gatacgtact cacccgcccc cgtgttcgat gtgccggcca
     4441 gcgccataaa ttcatcaagc gtcatgctgc gctgctcttt ttttcgtctg aaaggccaca
     4501 tatcacaccc ccgctaaatc cgcccaccag cggcgattat cctcacgcgg cattttttcg
     4561 gggtgctgct catacaggga acggcgggcc agctccacgc cggaatcggg gtaagccggt
     4621 accgatgtaa cggtaatttc gtacagttcc gccaccagca cggtgcgcac gcatggatct
     4681 gttgtggtat cccatacatc cttacgggag cgaaagctaa agctcatccc cgagatgtcc
     4741 ccacgcttaa ccagttcgat aacgtcgcgc cctgtactgg tatccggtgg ggtcagttca
     4801 aagcgtaacc ctgtttcgtc ctcttccagt ttcagcgtgc cggaacgggt gcgccccagt
     4861 aacatgctgt ggtcatgctc atacaggccg cgaacgtcat tacccgccgc aagccattca
     4921 gtaaacgccc cccgctggaa tttttcgtaa aactcacccc ataacagttc tgaaagctta
     4981 tcccagcgaa caacgtagcc cgtcagcgta ccggctccgc tggtggtgat ttccgatgac
     5041 cggatttcca tactcttcat aattttttca cccataaaac actaaagggg ctttttagcc
     5101 ccttctgtat gctgttaatc gtcgtcttcc ggcagttcca gaatcttgat cgcgttcgaa
     5161 tccaccacgc caccgcctaa atatttttgc gtgaaaattt tgatgaatcc tggttctgtc
     5221 aggttgtccg gtctggtgcg aacacctgtt tcgtgatcaa caatgtagta accgcgtttg
     5281 aagtcaccca gggcaataac gttatcaggc ataaactcca gatattcgac cggaaggccc
     5341 agcaacgtat caggatcacc cgcctgtaaa cggtcgcgcc agatataatc accgttcgcg
     5401 ttcttcacct tctgaagttt tgccgccgtc gtggagttaa ccacccagac cgcgtttttg
     5461 cggtattttt tacgtaatgc aaatttcagg tcgatcagcg ggtccgcaga tgtccacgcc
     5521 agagattcgg aaggtttaat tacctgcaac gtaccaaaat cacgctcttt gtcgttcttc
     5581 tctgcacggg gtacggataa aaaacctttt gcttttttgt caccgtcgcc cacaaccaga
     5641 tcgctttctt cggtttccgt gaaggtgtcg ccaatctcac ccgtcagcca tgaaaggatg
     5701 tccacatcgg aaaaatccac gatttcctgt gtggtgcgcg ggtacgcata gaccggatac
     5761 agcttaatgc tcacctcgtt aatctgcggg gtgctggtct gttcgcgtgc cttaccctct
     5821 tcaccgtggt taacggtcgc accgccagcg gaaacaagct gcttaaactc gttgctgctg
     5881 attttcttca cggtacagat gcggcgcata gtggattcat ccgccagcat tcgcatgatt
     5941 tcggtgttca gttcagggat aacggtataa ccaccatcag cgggaacgcc tgtacttaat
     6001 gcgcgggttt caccggtcag aatgtagttg cgtagttctg cggggtcagt ggtctggctg
     6061 cttttacctg gcttgctgcg ctcttcgtct gcaatggctt caaggcggga aatgtcttta
     6121 tcgagggatt cagctttagc gcgtaattcg tcaaattttg cgccctcagc atcgttaaga
     6181 ctgcggtttt ctttttcagc gttctccagc atgtcgcgca tctgattttt aatggcggtt
     6241 ttctgctggc gtaattcgat tattctctgc ataaaaaagt cctggggtta agtaaggaac
     6301 tccaggacgc ggcaaacact caaccgtttt tcataaggaa atcagtaatc gcaccgatcg
     6361 ttttcccgcc tggtaatgaa tattggcgag cacattaaca ggcgggaaag tggccccagc
     6421 gtcctggcac cacgggcgag aataatcatg attcagttcg ggtaaaatat gccgatccgc
     6481 tcagtgaaca acgtggaaca accacgaaca aataatttac aaaaaataac aaaaagccgg
     6541 attgctccgg ctgtatgtgc tgtatgggtg ctcagtctct gatccctttc agaaattcta
     6601 tcaatgcatc tgtctgttca ggatttactg ccagcatttc accggatagc gcgcaacgaa
     6661 caaaaccatg atgatccttt tcaatcagcg cccccgtttc cagaaatgcg cggtaatcag
     6721 tgatattcat cgtctccatg ttgtcagcat ggtatttatc acgctgttta agtatctcat
     6781 caaatttcat cggcattgcg ttttcctctg ttgtgtctgt ttgtttcaga tagtaactat
     6841 gccggaccgt gacgaaaagc cagtagtgct ccataccgtt tcaaatgacg caaaaaaagc
     6901 cggattgctc cggcttcgtg gtgtggttct gatattccta ctctgctaat ttgctcagcc
     6961 ccatgtctgc gtaagtgcga tttactgcat ttctcaggtc tgcgtagttc tccgttggcg
     7021 gctccggtgt gcccttgctg ctctgtttta ccagcgggtc gcccaccagc cgcccgtaag
     7081 ctgcaatttg tgctgtcata ttgccacctc agtgaagcga tccggcattg ttctttttca
     7141 tctcttccat cattttttca gtgagcatta cgcactgaaa ccccatgttt ttgatgtcta
     7201 cgggtttcat gcctttgtca gtggatatca caggcagaat atcctgatac acatcgcggt
     7261 cagggtaact aagcacacaa agcgcccatg atgccacggg tgaggatcct tcgtctgttt
     7321 caacccacca gcctggtgta gctgcgctta tttgcagaat cgtgatccgt ccgttgtgct
     7381 tgtaaaattt ttcaccaaat tttggttcgc tcatgattcc acctctccgg ttttaacgtt
     7441 gattgttgtt acctgttccg cttcggcaat ctcccgctct gtcagcgtgg caaaatttgc
     7501 cgccgtcgtg gtcatgaatg cgcttatcag ttcgggatgt gctttcgcgt atccttcccc
     7561 cgcgtggcgg tctatcgttc tgattgccac ctttaaagcg tgctctgtca tgtctaacgc
     7621 gcgatatttc ggttctgttc tgtctctgcg ttttttgagt gatttattaa atttccctga
     7681 agtgtgcata tttattttta ccccctcgtt taaaaagttt tgagttgtgc ctccccttgt
     7741 ctaccttagt ggccctcatg ccagtaatgg cgctgctttc agcggggtag agtgctttta
     7801 tccactatct accccgtgtc tacctccctg tctgattcag gtaaaatcag gtagagaggg
     7861 tagatagtgg gtagacagta aaaaaaggct acctacctaa cttaatgcac tgaattaaat
     7921 gtatttttct ttactcaggt agatagggta gacagcaatt acaaaaaatt ataaaaacgc
     7981 gtcgcactcg tctgttgtta ttgcgttagt ctgcgttact cccttaactt tccgcgtaat
     8041 atattcatgt ccgtaaactt tcgcggctgg cttcatagcc ttgccaaagt catttacgtt
     8101 tagcggtttg ctcctgcctg cgtacgccat aaacgccaga tagacgcggt aaaggctgtt
     8161 tctggtcgtg tacttcactg aatcgccacc gccgcccatc atcaggccgc gtgcttcctc
     8221 cagaaaattc aggaactggc aaaactcaat aaccggatcc gtctgttgct ttattgccag
     8281 agcttcatca ccgtcacgct gttccagtag taaagcccgt gctttctcag agtcggtaaa
     8341 gttcgccagc aatcggcgga taatgacagg gatttcagcc gcaatctttt ccggtagctc
     8401 cctgtctttt tcggcctcac taacgatatt gtcgaaacgg aaaatcacgc gacgacgtgc
     8461 cacacctccg gcccgttcgg tgaatatcat cgggttattg ttggtcgcca gcaccaccgc
     8521 cctgattaca gccgtgaaac gcttttcgta tttcgggtta atttccacgg ggtcaccgcc
     8581 cgtgattttc ttgatgcccg ttccttcgcc tgtatatttc ggctggtcag ccaggacgat
     8641 aagacgactc ccgacaacct gcggacgtcc accagcatca tcaagcgatg tcatttcagc
     8701 gcttaccgtg ttctgtttcc ctgccagaag gctggctatg tgtgtgaatg tacttttacc
     8761 gctcccgccg tctccggtgg cctcaataaa catctgccag tcgtaccggt tcgccataat
     8821 catgtacagc gcggcacata tacgcatcat cttgcgctgg tcttttccgg ctgcgtgctc
     8881 aagccattta tgaaagttta gcgcgttatc gcggatgttc tcccctggtg ctggtggcgt
     8941 gtactcaatg ccgttgtgcg tggtgatcca gttctccggc gtgtgcgggg aaaattcccc
     9001 cgttttcagg tcaagcgcac cattggcgaa cggcagcaaa tcgccggacg gctcgcccat
     9061 tggttcggca ataactttta acgcttccac ggcgttattg attacgcgct tgctgaaagt
     9121 ggccctgtgc tctgaataga tcgccaccat ttcgcggcta agttccattg tgctgaccgg
     9181 acaccatacc ccgccgcgcc atacgtgaac gatttcactt tcctgatgca cacaaacgcc
     9241 atcaaagcgc tcggcaagca gctgcgcgcg ctcactgtct gccatttgtg ccagttgcgt
     9301 atttttctgc tccggcatgg taagccctgc ggcaatattt tcacgctcag cattcagata
     9361 gcgccgccag ttttcacact tctggacgtg cattccttcg gggtaaaaat ttgcgtcctg
     9421 aatatctgcc gccgccagct tctgaccaat ctttttggtc tccaccaaat ccagttctcc
     9481 ggcctggtac agccttacgc gtttttttcc atccggaaca attttcagtg catcaagttc
     9541 ggcaagctga tttggcccaa gccacacagg cggcacatta tcgccggatg cggggccatc
     9601 ctgctcctgc cattgtttcg cgtgcgacca ggcatcacta cccgcaaaga taattacctc
     9661 tgtgtctttg tgttttattc cgcgtggctg tttttttacg ttcggtgcca gtttcatttt
     9721 ttacccctga atacgttaag catctttttt atttcctgaa tattggcgcg tgctttctcc
     9781 ctgctggttg gtttactgcg gggcgctgcc tgtaccagcg tgaaatcacg ccggaattga
     9841 taaacaggca tcacgcaatc atattcgtaa ccttcacggc ggtaggtgat gcgccgttct
     9901 gccacgcctt taatcgttac catgccgccg tactggtcgc ggtaaatatc accgcgcgta
     9961 aatttagggt gagtgttgcc actggcagtt aagccagaat atttaagttt cattattttt
    10021 attctccggt gtgctgttct ttatatctgt cgtgcaatag atctatttct tgcagttcca
    10081 ttattacagg ctcaagaagc gttattaatg ccgtggcaat tcttgatttt tgtttgtcgc
    10141 gttcattgtc gccaagtgtt tcaagccata tgcgcaatat ttccagcatg ttttcactgt
    10201 gagaaagtgc aagaaatgcg cggtctattg tttcgtggta aatatcacgc atattaatcc
    10261 ccgtccgtcg tttttcttaa aataacctct gtcacgaaat cagcataatc ggcggcgata
    10321 ttcagtacat caagcccgtt gatttatatt ctctcgtgga aagtaagaaa aaagccgctc
    10381 taataagttc tggcattgac gaaagcgcat cagccgcatc atcaggaacg ccggaaaatt
    10441 cctgtttcag ggaattaaaa cgatcatcac gcataacccc ccccattttc acaatcggca
    10501 acaagaatat ttttagcgtc attcagtgac cgcgttgcga tgtatcggat acattccagg
    10561 gcgaactgtg tccggtcttt ctgttcttct ccctgcgcaa gctctgccgt gcattcaata
    10621 tcaataagcg cgtgcatcag cgtagtgagt gcggcggctg ctgcgttcgg tgtggtttta
    10681 ttgtgcatag gtttccccct gacgaatacg ggcggcgaat acagcaacac aaccggacgg
    10741 gcaacggcta cgcgcttcgc gttccgtcca agcggttacg tggatgattt gggattctcc
    10801 ggtactcagt gccagaaaac gccacacaaa ggccgtttgt gtgtgtacaa ggtgtggtat
    10861 atgatttacg gcaaccataa cggctcctag tttacgttgt tggttagaag ccccgttact
    10921 gctcctacag tgcggggctt tgctttttgt gttgattgtg tgtaatgtgt cattacacat
    10981 aaatacatta catgaggtgt aattaacgtg tcaacacaca aaaacgaaag gcgtggtaat
    11041 cctccgttcc agttccgttt agatcctgac ttacgggaaa agatggaaaa ggcacaacaa
    11101 caggacggcg atgattctct tgctgcctgg ataaagcgaa taatccgcaa agaattacag
    11161 caacgcggca tagaacccaa aggataaaaa ctatcagcgc cgtggtgtga ggtactacgg
    11221 cgcattgctt tacaggcgca cacaatgacc aacgaagaat caaccgaaaa aacatcacct
    11281 gcacgaaaaa gacgacgcag aaagaaagag catgaatcag aacgattcgc gccgtgttct
    11341 tttgcccttg agaagtacct taaagaatac tcatcaaagg agagaacttc gcagttatgg
    11401 caacgcactg aaccagactg attgcattgc ccaccagcct gatagcggct atcatccccg
    11461 tgcttatgtt tgggatcaca cacacaaacg gcgcagcggg ttatctgttc agaaaggcgg
    11521 ctccatttcg gggccgcttt ttttatgcct gaaaaacccc aattttgtgg ttttccagtt
    11581 tcaccagggc gaacgaatcc ccgcccacgt tctggcgtat attcaatctt catggttata
    11641 gctctgtgtt cagatgattg atgtgtggcg gctgcgtgcc gccagcgtga ttaatgaact
    11701 gccttgcagc tatccttcca ggccagaacc tcggataaag accagccaac ggaacgaccg
    11761 ccaagtttac gacgtgatgg gaattgtccg gccttttcca ggcggtagcg gcatgagcgg
    11821 ctaaggcctg ttagcttttc gcattctttt tcacgtataa accgatcagt gcttaacact
    11881 attgccccct ttcgtttctt aaagagttat ttcgtgttct attgcgttgg gatgtgtctg
    11941 attgtgtcag gatgattcag agttggcaaa tgttgaggcc gtatggttta cagaaagagg
    12001 aataatcagg ataaaatcat ttaaattcat gttaatacaa aggcataaaa tattgtttca
    12061 tgcctttttt ctcgctattt aaagagtgat tcgctagtgt ataaaaaacc agtaagacgt
    12121 taaaaatcag ctacttataa atctgtacac tttttcacct cttgttcgta gttgttccgc
    12181 attgttgctc attgttgctc attgttgctc attgttgcac gttgtatctg ttcgcatatc
    12241 cagtatgcgc atactgaaaa aacacgaaaa aaattatttt cttctggcta ctggtagcgt
    12301 ggttacgttt tcatgtgttc ccgccagtat ccctaaccgc tccgtccaca tatccagcgc
    12361 attgcgttta gcatccagat aacgggaatg attataaact cgctgcattc ctggcatctg
    12421 gtggcctgta agctgctcca cgacgtgagg atccacgcct aaatcgttca gcatcgttgt
    12481 aaaggtgcgc cggatgtcat gtagtgacca gtgagggtga tttagcctcc tgtgcgctaa
    12541 tctgccgtac tgcgacacgc ttgtttcctg tttcacttcc cccagcaata agcccgtgtg
    12601 cctgttctgc tccaccagct gcgtgacgaa cggcagtatt gcttccggta tgggccggaa
    12661 tattgccacc ttcgttttgc tgtgttcttt cggcacggtc cagagcattt cggtaaaatc
    12721 ccactcgctg atctccgata acctcagttc taccgtccgg catccgaaca caatcaggag
    12781 gcggattaac gcgatgtagt agggggagaa tatttttttg tccagtgcct gcaataattc
    12841 gcccagttct ttggtgctta agacacgctc gcttatatcc ggttttttcc caacgtccgc
    12901 cacactcata tcatcaagaa cgttgctgat tgcatagcgc cgcctccggc agaacttaag
    12961 cgcctgtttg cacgtctgta gcaagaatcc ggcagtaaca ggcgttcgct ttgccacctg
    13021 gtcaaaacag gccagccagt gccgtagctc gcatttatcc agcggcatag caccaatgtg
    13081 ctgtattacg tgattattaa ggcgcttttt cagggcggca taatccacgc ggttttcctt
    13141 tgcgtacgac tcaagccagt aggtgagcgc atcgccaacc gttaccggct ttaacgcttc
    13201 ctgtacggtg taattaagct catgacgtgg atttttcccc tctgccagcc atgcgcgaca
    13261 ctgggcggct ttttccctgg ctgatttcag gctcagatca ggataatttc ccagcttaat
    13321 gcgttccggt ggtgcccccc ttcccgttcc ggccctgtaa gtgaaatacc aggttaaaag
    13381 gccactggtt gaatgcctga cgctcaggtt tccaccgtca ttaagaaagg ctgttttttg
    13441 ggcgggtgtg ccgttgattt tcctcagctg tgtatcgctc agtttgttaa gtgctctgct
    13501 cataattttg attccggtca tcacaattaa ctacaccatt aactacaccg atcgttgcac
    13561 aaagggctac aacgtgaaac aagctggaac aaggaaaatc acaaatctgt ttataatcaa
    13621 aaacataatg aacaatctga aacattatga aacggcaaaa aacactaatt gataaaatat
    13681 gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
    13741 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
    13801 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
    13861 cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc
    13921 aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa
    13981 ccaactggtc attactggta caggcagtaa accactggca tgagccgacc gccgcgctga
    14041 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg
    14101 tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
    14161 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc
    14221 cagatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg
    14281 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
    14341 actattccgt gggttttcgc gcgccaaata cgcgggaatt aattagcgga tttgccgatt
    14401 atgtgctgca acgtgaactg ggcggcaact actacagcga tccggatgtt ccacctcgcg
    14461 ctcatcctgc ggatgttctg ccgcaagaga tggataaact gcgtgagatg atgctcgaat
    14521 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
    14581 atgaactgga tatcgcgccg ccggaaccgc cttatcagcc agatgaaatc tacgatgcac
    14641 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
    14701 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg
    14761 ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
    14821 tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga
    14881 tttaagccgg atgcagcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
    14941 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag aatcacaaac
    15001 tcatgcttac catgatagtt gtaaccgcca gtgaacaggt tcgggcaagg taatcccata
    15061 aacgacaact gcgcgccgtt ggtaccgccg cggatcggtt tcagttccgg ttcaatatcg
    15121 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctcg
    15181 cgcatattgt agtaactgtc ttcaatcacc aattcaatgt agcaatcagg atgtaaccct
    15241 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
    15301 tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc
    15361 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
    15421 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
    15481 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
    15541 agttcgccta cgccaccacc gtcaacagtg taagcccagc gggcatcgaa ggcatcaaca
    15601 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    15661 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct
    15721 gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    15781 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    15841 atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc
    15901 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    15961 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct
    16021 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    16081 ctcacccctg cttttgattg ggtatccaga gacacgtagt tcaaaaatcg ctcaagtagt
    16141 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    16201 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    16261 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    16321 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    16381 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    16441 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    16501 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    16561 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    16621 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    16681 tccaaagcta tgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    16741 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    16801 tgcagctgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    16861 gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    16921 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    16981 gtaagcttgg cattacattc gttttcgtga ctcacgacca ggaagaagca ctcaccatgt
    17041 cggacaggat tgtggtgatg cgcgatggtc gcattgagca agacggcacg ccgcgtgaaa
    17101 tctacgaaga gccgaaaaac ctgtttgttg ccgggttcat tggcgaaatt aatatgttta
    17161 acgccacggt gatcgagcga ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    17221 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    17281 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    17341 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    17401 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgatcctgac tttgaccact
    17461 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    17521 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    17581 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    17641 agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    17701 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    17761 ctgggctatc catttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    17821 ctgtttctgc tgattgttcc gttctggacc aactcattga ttcgtatcta cgggctgaaa
    17881 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    17941 acaccaatcc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    18001 ctgccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    18061 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgcat cattattcca
    18121 ctgacgatgc cggggattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    18181 ttctatgtgt ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    18241 gtacagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    18301 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtttgct gaataagaag
    18361 gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt
    18421 acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca
    18481 tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac gatagcctgt
    18541 tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacgttt gctacgctta
    18601 tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgcggtaag ccgttcgtta
    18661 gcggaatgct gtttgtggta atgatgtcac cagatatcgt gatggcaatt tcgctgctgg
    18721 tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca
    18781 ccttctgcct gccatttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc
    18841 ggatgctgga agcggcgaaa gatctcggtg ccagcgaatt taccattctg cgaaaaatca
    18901 ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga
    18961 tggacgatgt ggtagtttct tcattcgtca ccggaccaag ttatgaaatt ctgccgttaa
    19021 aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat
    19081 tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga
    19141 aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc
    19201 tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa
    19261 ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa
    19321 ggttatctat tcgacttacg agtcgaacga aaccatgtac gccaagctga aaacgtacaa
    19381 agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga
    19441 ggggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga
    19501 catgctcaac aagccttttg acccgaataa cgactactcc attccgtata tctggggtgc
    19561 gacggcgatt ggtgttaacg gtgatgcagt ggatccgaaa tctgtcacca gctgggccga
    19621 tctgtggaag ccagagtaca aaggcagcct gctgttgacc gacgatgccc gtgaagtgtt
    19681 ccagatggcg ctgcgtaagc tgggctactc cggtaacacc accgatccga aagagattga
    19741 agctgcatat aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa
    19801 cccggctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggttctgc
    19861 tttcgttgca cgccaggcgg gtacgcctat tgacgtggtg tggccgaaag aaggcggcat
    19921 attctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa
    19981 attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgctgaaa ctatcggtta
    20041 tccaacgcca aaccttgcgg cgcgtaagct gttaagtcca gaagtggcaa acgataagac
    20101 gctctacccg gatgctgaaa ccattaaaaa tggcgaatgg cagaatgacg ttggcgcagc
    20161 cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat tcaacaagcg
    20221 gacggtggta ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag
    20281 taaagtttta tcccgctctt tcgtcggtat tgtggtccgc ttgtaggcct gataagcgta
    20341 gcgcatcagg caatgcttcc tgctttataa tccctccagc aacttttcaa caaattctgg
    20401 caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccaa cctgactcgg
    20461 ttcaagattc agttcaacgg tgtgagcacc atgtagtttg gcttcgtgta caaacccagc
    20521 agccggatag acatgcccgg aagtaccaat ggcaataaag acatccgcca tcgacaacgc
    20581 catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcggacgcaa
    20641 gggtgccggg aagtggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc
    20701 gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca tatgaatgac
    20761 attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca gcaaaaagcg
    20821 atcgccgaga gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc
    20881 tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg
    20941 atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc
    21001 ggcgcgaaag gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac
    21061 tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa
    21121 acgctcgcgc aggcgacgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga
    21181 cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc
    21241 tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg
    21301 acaaggcaac ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc
    21361 aatgacgacc aggtcagggt caacaatggt caggatattt cccagagaaa ccgctaataa
    21421 atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat
    21481 aatttcggga gcctgcaacg gttgatgata atagtgttga tacagccacg caaaaccgcg
    21541 accagacaga taattttcaa tgcagccatg ctgaccacag ccgcagcggc gtaacgggaa
    21601 atccaacccc atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt
    21661 aatgtagctt ttaccagtaa tcggtttgcc gttgaaaacc agcccgccgc caacgccggt
    21721 gccgagaatt aaccccatca ccagtggata ttgcgtaaat tcatcctccc aggcttctga
    21781 aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag
    21841 gtcggcacgc agcggtttgc cgctggcagc ggggacatta gcggcataca gcttaccatc
    21901 ttctgtttcc ggcattcccg gaataccgat gccgacagaa cctttacagc caaaacgttg
    21961 gtcagcttca gctaccagct cacacactgc atctaaaaat gcgtcatagc tgtcacgcgg
    22021 tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat
    22081 ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc
    22141 tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc
    22201 aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt
    22261 gcaattccga tggcaggaag tcaataaaat agatatcgct ggagaggaac tgatgaccga
    22321 tcagcttttc aatccactca ataatcgggg taagttgcag tgaaacaact acgccgataa
    22381 ccaccccaca caggctgccg aacagccctg ccagcaatcc ataccagaca aagatggcgc
    22441 gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt
    22501 tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca
    22561 tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac
    22621 caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct
    22681 tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat
    22741 caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac
    22801 tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct
    22861 cgggattcga gttggggatc atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat
    22921 ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccacgcat
    22981 ccccctgaac aaacgagggt aatgcgctca gacgctgttc ctgttgtggg ttaacgccct
    23041 tcacctggat tgcgcgcaga ttcgctccac tttccaccag accggtgaaa ttgatatacg
    23101 gcgcagcggc ggcaataccc ggcactttct gcacgttatc cagtgcttcc tgccagttag
    23161 tccatggctg atttaccgct tcgatttcgc catgcggcac caccgccaga atgcggttat
    23221 tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga
    23281 gggcaatgcc aatggtagaa atgacagaga ttagcgacac catgccgccg cgtcgccgtc
    23341 cgcgactaaa acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat
    23401 caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt
    23461 cgccagttgc aggtcgtgag tgaccaccag gaaagcggtg ccctgcaagc gattcaactc
    23521 tccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggttcatc
    23581 cgccagtacc agacgcggat tattgaccag cgcacgggca atcgctacac gctggcgttc
    23641 gccgccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa
    23701 catctccagt gcacggctgt tgatttcagc gggctttttc ttgccaatca gcagtggcat
    23761 agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc
    23821 cagcttctgg ttgcgcagtt ctgctttcgc cgctgaagac agtttgctca tcggctgacc
    23881 gttaaagatc acatcgccgg aggttggcgt atccagcccg cctaacaggt gcagcaaggt
    23941 acttttaccg gaaccagagc taccgacaat tgccatcatt tcaccttcgc ccacgctgaa
    24001 actgacattg tgcagcacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt
    24061 gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttgt gtggcggcag
    24121 cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa
    24181 caatgacctg taaaggttcg atagccaccg gcagcgccgc gccatcaagc aggacgccga
    24241 ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc gctccgagga
    24301 ttgcaccgat aatcccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg
    24361 gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg cttctccatc accatcagcc
    24421 ccagcgaggt aataatgtta aacgccgcaa cagcgacaat caggctcagc agtaagccca
    24481 tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct
    24541 gccatttgct gccttcaggc agtttttgct gacttaaaga gtcaactttc agcggctcat
    24601 ccagccacaa acgccagccg gtaatattgg ctgccggata acgcatcaga cgcgaggcat
    24661 cctcaatatt caccagcatt tcatagccat cgacttcact gttggcggcg aaagtgccaa
    24721 tcacattgaa caggcgctga cttggaatac gccccatcgg cgtgaactgg ctggcagatg
    24781 gcaccatcac gcggatttga tcaccgcgat tgacgcccaa ctgtgaggca agttgttcgc
    24841 cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg
    24901 gcgttagtgg atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc
    24961 gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca
    25021 gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa
    25081 ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca
    25141 ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg
    25201 agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcaag ccaataaata
    25261 gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa
    25321 gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    25381 cccccgtaaa tcaatcggct aaattgccat gttttatcat caatcttgcc gcctgatctt
    25441 aatctgaaaa atcatccaat acagacagat acccattaca cctcgggtca taaccagcag
    25501 gatcaaccgc atgtaaatta aaaaagagcg tgttgttctg atgaaacaaa aagagctatg
    25561 gattaaccag atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac
    25621 cttttatccg catatgacca cttttcagca tccgttatcg gaagtcctga gcaaatgctg
    25681 gatctatttc aatctttacc ttgccccctt tcgtatgccg gtttttttct ttatatccgg
    25741 ctatttgatt cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat
    25801 ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cacctgagcg
    25861 cgatttaagc aatgcctcca atgccgctta tgccgattcc accggtgagt tcctgcacgg
    25921 gatgatcacc gccagcacca gcttgtggta tctgtatgcg ttaattgtct atttcgtggt
    25981 atgtaaaatt tttagccgtc tggcgctgcc actattcgcc ttgtttgtac tgctgagtgt
    26041 ggcggttaat ttcgttccca cgccgtggtg gggaatgaac agtgtgatac gcaatttgcc
    26101 ttattacagc cttggcgcat ggtttggcgc aacaataatg acctgtgtta aagaggtgcc
    26161 gttgcgccgc catctgctga tggcttcttt gcttaccgtt ctggcggtcg gtgcctggtt
    26221 gtttactatc tcgctgctgt tgtcgctggt atcgattgtg gtgatcatga agctgtttta
    26281 tcagtacgag caacgattcg gtatgcgctc caccagcctg ctgaatgtga ttggctccaa
    26341 caccattgct atctacacca cccatcgcat tctggttgaa atattcagct taactctgct
    26401 tgcgcaaatg aacgcagcac gctggtcgcc gcaagtcgaa ctgacactcc tgctggttta
    26461 tccctttgtc agtttgttca tctgtactgt tgcgggcttg ctggtaagaa aactttcaca
    26521 gcgtgcattc agcgatctgt tgttctcccc gccttcactg cccgctgccg tcagttactc
    26581 tcgttaaacc atcaccccag ccggggcgat tcaatttgct aaaccatgtc gattacggat
    26641 aatgaacagc attgcttatc agagatatgc ccccatatgt tgaggcatat cctaacgaga
    26701 atctgacaac cgttatgcct gaacaatatc gttatacgct gcccgtcaaa gcgggtgagc
    26761 agcgtctgct gggcgagtta accggcgcag cctgtgcaac gctggtagcg gaaattgccg
    26821 aacgtcacgc cggtccggtg gtactcattg caccagatat gcaaaatgct ctgcgtttgc
    26881 atgatgaaat cagccagttc accgatcaga tggtgatgaa tctggcggac tgggaaactc
    26941 ttccctacga cagtttttcg cctcatcagg acattatctc ctcgcgcctt tccacccttt
    27001 accagctacc gacgatgcag cgtggcgtac tgattgttcc ggtgaatacg cttatgcagc
    27061 gcgtttgccc acacagtttt ctccacggtc atgcgctggt gatgaaaaaa ggtcagcgcc
    27121 tgtcacgaga tgcattacga acccaactgg acagcgccgg ttatcgctat gttgaccagg
    27181 tgatggagca cggcgaatac gccacgcgcg gcgcgttgct ggatctcttc ccgatgggga
    27241 gtgagctgcc ttatcgtctt gatttctttg atgatgaaat cgacagcctg cgggtgtttg
    27301 acgtcgacag ccagcgcacg ctggaggaag tagaagcgat caatctgctg cccgcacacg
    27361 aatttccgac cgataaagcg gcaattgaac tgttccgcag ccagtggcgc gataccttcg
    27421 aagtgaagcg cgatccggaa catatttacc agcaagtgag taaaggcaca ttacctgccg
    27481 ggatcgagta ctggcagcca ttgttcttca gcgaaccact gccgccgctg ttcagttatt
    27541 tccctgccaa taccttgctc gtgaatactg gcgatctgga aaccagtgcc gaacgtttcc
    27601 aggctgacac gctggcgcgt tttgagaatc gcggcgtcga tccgatgcgc ccgctgttgc
    27661 caccacaatc gctctggctg cgggtggacg agctcttctc agagctgaaa aactggcccc
    27721 gagtgcagct aaaaactgaa catttaccga caaaagccgc gaatgccaat ttaggtttcc
    27781 agaaactgcc agacctggcc gttcaggcgc aacaaaaagc gccgctggat gcgctgcgta
    27841 agttcctcga gactttcgac ggtccggtgg tgttctcggt agaaagtgaa ggtcgccgtg
    27901 aagcgctggg tgaactgctc gcacgaatta aaattgctcc gcaacgcatt atgcgtcttg
    27961 atgaagccag cgaccgtggg cgttatctga tgattggcgc tgccgaacat ggttttgtcg
    28021 ataaggtgcg taatctggcg ctgatctgcg aaagcgatct gctcggtgaa cgtgttgccc
    28081 gccgtcgtca ggattctcgc cgcaccatca accccgatac actgatccgt aaccttgcgg
    28141 agctgcatat tggtcagccg gtggtccatc tggagcacgg cgtcggtcgc tatgccggaa
    28201 tgaccacgct ggaagctggc ggcattactg gcgagtacct gatgctcacc tatgccaacg
    28261 acgccaaact gtatgttccg gtgtcgtcac tgcatctgat tagccgttac gcaggtggcg
    28321 cggaagaaaa cgccccgctg cataaacttg gcggcgatgc gtggtcacgc gcgcggcaga
    28381 aagcggcgga aaaagtgcgt gatgtggcgg cggaattgct ggatatctac gcgcaacgcg
    28441 ccgccaaaga gggcttcgcg tttaaacacg atcgtgagca gtatcagttg ttctgcgaca
    28501 gcttcccgtt tgaaaccacg ccggatcagg cgcaggccat taatgcggta cttaacgaca
    28561 tgtgtcagcc gctggcaatg gatcgtctgg tgtgcggcga tgttggcttt ggtaaaacag
    28621 aagtggcgat gcgcgccgct ttcctggcag tagataacca caagcaggta gcggtgttgg
    28681 tgcctaccac ccttcttgcg cagcagcatt acgacaactt ccgcgaccgt ttcgccaact
    28741 ggccggtacg tatcgaaatg atctcccgtt tccgcagcgc caaagagcag acgcaaatcc
    28801 ttgcggaagt ggcggaaggg aaaatcgata ttctgatcgg tacgcacaaa ctgctgcaaa
    28861 gtgacgtcaa gtttaaagat ttaggcctgc tgattgtcga tgaagaacac cgcttcgggg
    28921 tgcgtcataa agagcgcatt aaagcgatgc gcgcgaacgt ggatattctg acgcttactg
    28981 caacgccgat cccacgtacg ctgaatatgg caatgagcgg aatgcgtgac ctgtcgatta
    29041 tcgccacgcc gcccgcccgt cgtctggcag ttaaaacctt tgtccgtgag tatgacagcc
    29101 tggtggtccg ggaggcgatc ctgcgtgaaa ttttgcgcgg aggacaggtt tattatctct
    29161 acaatgatgt ggaaaacatt cagaaagccg ccgaacggct ggcagaactg gtgcctgaag
    29221 cacggattgc catcggtcac gggcagatgc gcgagcgcga actggaacgg gtgatgaatg
    29281 atttccatca tcaacgtttc aacgtgctgg tttgtaccac cattatcgaa accgggatcg
    29341 acatcccgac agccaacacc attatcattg aacgtgcgga tcacttcggt ctggcgcagc
    29401 tgcaccagtt acgcggtcgc gtcggacgtt cacatcatca ggcatatgca tggctgctga
    29461 cgccgcatcc aaaagcgatg actaccgatg cacaaaaacg tcttgaagcg attgcctcgc
    29521 tggaagatct cggggcaggt tttgcgctgg caacgcacga tctggagatt cgcggcgcgg
    29581 gtgaactgct tggcgaagaa caaagcggct caatggaaac catcggtttc tcgctgtata
    29641 tggagttgct ggaaaacgcc gtcgatgcac tgaaagccgg acgcgagccg tcgctggaag
    29701 atctcaccag ccagcaaaca gaagtcgagc tgcggatgcc gtcgctattg ccagatgatt
    29761 tcatccctga cgtgaacacg cgtctgtcgt tctacaaacg tattgccagc gccaaaacgg
    29821 aaaacgaact ggaagagatc aaagtcgagc ttatcgatcg cttcggcctg ctgccagatc
    29881 cggcgcgtac cctgctggat attgcccgtc tgcgccagca agcgcagaaa ctggggatca
    29941 ggaagctgga aggtaatgag aaaggcggga tgatcgaatt tgccgagaag aatcacgtta
    30001 atccggcctg gttgattggt ttgctgcaaa aacagccgca gcattaccgc cttgatggtc
    30061 cgacgcgcct gaaatttatt caggatttga gtgagcggaa aacgcgtatc gaatgggtac
    30121 gccagtttat gcgtgaactg gaagagaacg cgatcgctta atgtaaatcg ggggcgtttc
    30181 tgcgccccca tctgcaacat ttacaaattc tttgcacttt cctgcactat ccgacactgt
    30241 caccatccat aattcaggct tatctgttta ttacaataac cttatattta ttatggattt
    30301 ttggtgatga tcaaaacgcg tttttctcgc tggctaacgt tttttacgtt cgccgctgcc
    30361 gtggcgctgg cgctaccggc aaaagccaac acctggccgc tgccgccagc gagcagtcgt
    30421 ctggttggcg aaaacaaatt tcatgtggtg gaaaatgacg gtggttctct ggaagccatc
    30481 gccaaaaaat ataacgtcgg ctttctcgct ctgttacagg ctaaccccgg cgttgatcct
    30541 tacgtaccgc gcgcgggcag cgtgttaacg atcccgttgc caaccctact tccagacgcg
    30601 ccgcgcgaag gcattgtgat caacattgcg gagctgcgtc tctattacta cccgccgggt
    30661 aaaaattcgg taaccgtgta tccaataggt attggtcagt taggtggtga cacgctgaca
    30721 ccgacaatgg tgaccaccgt ttcagacaaa cgtgcaaacc caacctggac gccaacggca
    30781 aacatccgcg cccgttataa agcacaggga attgagttgc ctgcggtagt gccggctgga
    30841 ccggataacc caatgggcca tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg
    30901 cttcacggta cgaacgccga tttcggcatt ggcatgcggg taagttctgg ctgtattcgt
    30961 ctgcgggatg acgatatcaa aacactcttt agctaggtca ccccaggcac caaagtgaat
    31021 atcatcaaca ctccgataaa agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta
    31081 catcagccgc tgtcagagaa gattgatgac gatccgcagc tgctgccaat tacgctgaat
    31141 agcgcaatgc aatcatttaa agatgcagca caaactgacg ctgaagtgat gcaacatgtg
    31201 atggatgtcc gttccgggat gccggtggat gtccgccgtc atcaagtgag cccacaaact
    31261 ctgtaaatcc tggataaaaa aaatccccgc gacacggcat gtcgcgggga aaaaatcagc
    31321 agtagcaggc attaatgagg gttaatgctt atttataaat tactgctgtt ccatggaggg
    31381 tattcggacc ggttacagaa gtaatacgga aagattttgc gcccatctca tccgcttttt
    31441 gcgccagctg ctcttccagc gatcccagat ttgtccccgc gttagcactg atagtaccga
    31501 ctttttgttg gccttctggc gttgactgaa cttcgacagc cgcaaagttg gcaaatgaca
    31561 tggagcttaa aatcgccgca gcgatgaggg tttttacgtt tttcataata gtggccttat
    31621 gcagatgaat gacgtcgcaa gaaattactt aacgatcgat aactaaatga tagatgtgat
    31681 ctggatcaca tacaagatat tttttataac aatcattaat taatttaaaa atctttaaat
    31741 ttcagtcaaa tagaaagcaa ttatttttat gggatctgga ctggtgaacg gaggtgatta
    31801 tttttataat aacctttcat tcacagtcac caggtacaac ggcatcatgg caactgactc
    31861 aacacaatgt gtaaaaaaaa gccgtggccg cccaaaagtg ttcgacaggg atgccgcgct
    31921 tgataaggcc atgaaattgt tctggcaaca cggttatgaa gcgacttctc ttgcggacct
    31981 cgtcgaagcg accggagcca aagcgcccac gctatacgcg gaatttacca acaaagaggg
    32041 gttatttcgt gccgttctcg accgctatat cgatcgtttt gccgctaagc atgaagcaca
    32101 gctgttttgt gaagagaaaa gcgtggagtc tgcgctggct gactattttg ctgccatcgc
    32161 caactgcttt accagcaaag acaccccggc aggctgcttc atgatcaaca actgcaccac
    32221 cctctcccca gattcaggag atatcgccaa tacgttgaaa tcacgccatg cgatgcaaga
    32281 gcgcactttg cagcagtttt tatgtcaacg acaagcgcgc ggggaaatcc cgacccactg
    32341 tgacgtgaca catctggcag aattccttaa ttgtattatt caggggatgt cgatcagcgc
    32401 acgcgaaggt gcatcgctgg aaaaactgat gcagattgcc ggaacgactt tgcgtttatg
    32461 gcccgaactg gtgaaataag tcgcaaaaaa agcccctcag cctgagcgaa tgaggggcca
    32521 aattgcagag tacagccttg ttatactttg ttattcaaaa tcagttgccc gttactatct
    32581 agcgggatct gcgtacccgg atcgcggtcc atgcggattt tgccctgctg atcgccaatc
    32641 ttataggtca catcataacc gagcattttt tctgacttgt catacaccgt tttacaacgc
    32701 tgttgcgtag tcgtgtaagt atcgctttcc tggagagagc cctggatctg gttaccggca
    32761 tatccaccac ccagcgcccc cacaacagtg gcgacatctt taccgcgacc accaccaaac
    32821 tgatgcccta tcacgccacc agcaacagcg ccgagcaccg acccggtaat gcgattttca
    32881 tcctgcaccg gtcgacgatg ggtcactgtg acgttgcgac actcctgacg cggtgtttta
    32941 accgtttcct tgattggggt tgcagaaaca acctgagcgt attgcgggcc ccgttcaaac
    33001 acgttcagac tggccactgc cgctacgccc aacgcagctg cgacaccaat cccgataccc
    33061 gccaacattg atttattcac gggaacacct ccttctttgc agttatgcca atcatgcaag
    33121 cgaaggagaa aagcagctat cagacagaag atgaaaagcg cgggaatgac tggaaatact
    33181 taaggattcg gaggagtctg aaggaatgcc tgatgcgacg cttaacgcgt cttatcaggc
    33241 ctaccgctcg taccatttgt ccagggcgga taaggcgttt atgccacatc agccagtgta
    33301 cgtcgattaa tgcaacttca aacgcggacg gataacgcgg ttaatactcc ccaccagcat
    33361 cattaatccg gttttaaagt aaccatgcag cgcaatctga tgcattcggt atagcgagat
    33421 atatacaaag cgcgcaattc gtccttcaat catcattgag ccgcgcgtca ggttacccat
    33481 caggctacca acggtggaga agttcgacag cgataccagc gaaccatgat ctttatactg
    33541 ataatttttc agcggcttac cgttcatctg cgccagaatg ttgttcattg cgcaagtcgc
    33601 catctggtgt gcagcctgag cacgcggcgg aacaaagccc ccttccggac gcgggcatga
    33661 cgcgcagtcg ccaatagcgt aaatgtctgg atcgcgagtg gtttgcagcg tcggttccac
    33721 caccagctgg ttgatacggt tagtttcaag accaccgata tctttcagga agtctggcgc
    33781 tttgatcccg gctgcccata ccatcagatc agcctcaata tattcgccat ctttagtgtg
    33841 caggccgcct tcatcagcac tggtgaccat ggtttgcgtc agcacgcgaa cgccaagttt
    33901 cgttagctcg ttgtgggccg cagcagagat acgtggcggt aatgcaggca aaatacgttc
    33961 tcccgcttct accagcgtta cgttcagggc ttcgttggtc aggcctttgt aaccgtagct
    34021 gtgcagttgc ttgatcgcgt tgtgcaattc agcggagagt tctacacccg tcgcgccgcc
    34081 gccgacaatc gcaatgttca ctttgccatt tgcgcccagg ttggcagagt atttcaggaa
    34141 cagattcagc atctcctggt ggaagcgacg cgcctggtgc gggttatcga ggaaaatgca
    34201 gttctctttg acgcctggcg tattgaaatc gttagaggtg ctacccagcg ccatgaccag
    34261 ggtgtcatag gcgattttac gttccggaac caacagttca cctttctcgt cgcgtagttc
    34321 tgcaatagtg attgttttcg cttcacgatc aatatcaatg acggaaccca actggaactg
    34381 gaaaccatga ttgcgcgcat gggccagata gctcaacgca tcgacgcctt catcaagcga
    34441 gccagtcgcc acttcgtgca gcagtggttt ccacaggtgg ctgtggttac gatcgaccag
    34501 cgtaattttg gcttttttct tacgtcccag cttatgcccc agctgtgttg ccatttccag
    34561 cccaccagca ccgccgccga caatcacaat ctttttcaat ggcgtagtca acgtgacccc
    34621 cttaaattta ttaaccaatt gttaattaat ggttattaac atagctctta attaacaaca
    34681 ggttacagca atgaaaatgt tcttcgctat tgagaataac atgaatggtg cattggtcat
    34741 accaaaattg atgttaatca agttttgttg cttaaaagat aggcaacttt accgggggag
    34801 atcaaaaaat agcgtttatt atttgagcaa attcggaaga gtaatatagg gtgaagcgta
    34861 tatcgagggc gtttggtcgt cctgataaga tgcgcagcat cgcatcaggc attgatgctc
    34921 gactgtcgga tgcggcgtgg acgccttatc cgacttacag aattcggagc gcatcaggta
    34981 ccggcaaggt tacctgatgc gacgagggca tttacccgag ggttttgaac gctttaatgc
    35041 gctgtaaatg cggggagata ttcttgaatt tgtgcgtctg ctcttcgtcc cagacaatct
    35101 cgtaataatg atgcaactct tcagatgtcc gctggctgtt aagtgcttca tcattacgcg
    35161 acaaaataac cagacaacga tcgcgattct tctcgcggaa gttggtcaca cacttagtgg
    35221 caatatcggc atactcttcc gggcgatcaa tcttgccttc catgttctca taagggaaca
    35281 aattagggtt gaagatcacc tggcggatat cgcaaagaaa accaatccgt tccgcccagt
    35341 atccgcccag gccaacgccg caaattagcg gacgctcgtc aacgttcagt tgcaacattt
    35401 tgtccacttc tttaagcaga tgctgcatat catgtttcgg atgccgcgta ctgtagctta
    35461 tcaagcgtac atccgggtca ataaactgca attgtaagac tttctcgtgg ttacccggac
    35521 tgttagagtc aaaaccgtgt aaatagataa tcatcgcttc ctcacataag ccagggttag
    35581 tgacctgctt tctcttcctg ccagcgttca tgtaactgat tcagacgggt gctgatcgct
    35641 ttccagcgag ccgagtccat cagttcctgt cgcgaaaatg aacctttatg atacaaacgt
    35701 gtaacacgtt ctgccttgat cggtgacaga ttatctaaca cgctgacggc ccctttacga
    35761 ttattgcaga ccaggatcat atcgcaaccc gcatccagtg atgcctgccc gcgttcggca
    35821 taactgccca taatcgcggc accttccatc gataaatcgt cagagaaaat cacgccgtca
    35881 aaacccagtt cctgacgcaa aacggttttc agccagtagg gagaaccgct cgccggacgc
    35941 ggatcaacat cactgtagat cacatgcgca ggcataatgg cgtcgagttt attttcgcgg
    36001 attaaggaac tgaagaccga catatcttta gcgcgaatct ccgcttgtgg acgtggatcg
    36061 cacggtgttt ctttgtgtga gtctgccgtt actgcaccgt gtcctgggaa gtgtttcccg
    36121 gtcgttttca ttccggcttc atgcatacca tcaataaacc ggctggcaat tgccagggct
    36181 ttttgtggat cggcatgata agaacgctcg ccaatcgccg cgctgatatg cccgacatcc
    36241 agcactggcg caaagctgat atcaatatcc atagcgatca tttcgctggc catcagccaa
    36301 cccgcctctt gcgccagttt gccgccctct tccattccca acagcgcagc aaaggattgt
    36361 gctgccggta agcgggtaaa accttcgcga aaacgctgca cgcgtccacc ttcctgatct
    36421 accgccacca ccagatgatt gcgcgatgct gcgcggatct ggcgcaccag ttcacgtaac
    36481 tgggcaggat catgatagtt acgcgtaaag agaatcagcc ctcccaccag cggatgcgcc
    36541 agtatttcac gctcttccgc gtccagttcg taaccttcga catccaacat tactggaccc
    36601 acactgctct ccttattgtt ttattaatag ctgccgccag gtgtcatcgg ccagcctgat
    36661 aaattgttga tcgccggttt gtcgccagcg gtactcaaac caccctcctt tgagcatcag
    36721 cagccaggga aaccatcgcc tgacctgacg ccataattgc gccggataaa tcttcgcgcg
    36781 agtggcatag tcattgacca attgccggtg ctgttcagta ttttccaccc acaccgccgc
    36841 cagttccagc gcgatatcac catctccagc atactcccag tcgatgagtt ttaaccctga
    36901 tgcgctatgc actaaatttc cggcgtggac atccatatgc aatggactta agcgtaaagg
    36961 tcgtggttcc cgcgctttgc gcagacgttt taacattcgc agccaaccca cagtccgccg
    37021 cgccggatcg ctttgctgcc agtacagttc cagtaagggc aacagcgtta ttcgccagcc
    37081 aaaacgtggt tgttgatgta gataatacag caagcctgcc agttcgttgg tatccggcaa
    37141 atacgttttt accgcgccgg gcagatagtc gactaccatc cagtcacgga gatataaatg
    37201 cggcttcggt gcaatgcttg cgggtagttg tgataaagcc cgatactggc gcaagaacgc
    37261 ggactgcggc gcatcaggat cgtgcggctg acgcacaaca aaacgctggc cctgatgttc
    37321 aatgagaaaa ctaccgccac taagcccact attaaacgtc gtgacgggat gaaactgcgg
    37381 gaaaaagcgc gacagcaatt cgtcgcgcgt gatgggatta ttgctgcgaa acggcacctt
    37441 tacctgacca gataatttcg cccgtctgca ccagcatcag ctgcatttgt agggtcggag
    37501 cgttaacgtt gccagaggcg ctggagtaca gcacgtaatg agcgccgaca ttgcgggcaa
    37561 tgcctatggc tttactacgg gtgcctaaac tgtcctgcgg cgacaaacct aactgttgct
    37621 tagccatcga cagttgctgg gcggaaacca gggtaaattt cccgttatta gccagcgcat
    37681 ttcgcagcgt ttcggtcgct tctgcggcat tcagcgaacc gttagtacgg ttgttaacgc
    37741 tatcaaccag caggacgcta cccgcagtca ccccgtcagc cccaagcatc ttactgacca
    37801 tcggctgcat tgcgccattc cagtcataat ggcgaatatg cggcgcaggc ggtgccgttc
    37861 gatcttcgtg ctcaattggg cctggctgct gcgggatcgt cggcaccgag ggcactgtgg
    37921 ggacaggctg ttgtggctcg gctggttgtt ccggcgctgg tttcacttct tctaccggtg
    37981 caggttcacg ttgccccaca cacccggcga gaaacatcgc cagcgcggta atcaaggcgt
    38041 agcgactcat ttttgtcatc aagattcacc cctttacaaa tatagataaa gtctgacttt
    38101 gtgcgccccc agaaaattgg cgctgccgta cagcgttacc gccgaatgtg cgggaatggt
    38161 aacgctgcgt ggcctttcca gaggatgcat ctccagccct ctggcgtcat accagtaaaa
    38221 acgataatga acggtaacgg gttcttgcct ttcgttataa agcgttgagg atgctgaagg
    38281 ttgaatatca gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag ccaataaaga
    38341 tgactccatc accagcgatt gctcatcatt caccggaatt tccggatgtg aacgacagcc
    38401 caccagcagt aacaacacca gagacaccag cccaaagcat ccttttctca tcgttacaga
    38461 cctttatgcg ccagcattgg tcccagcgga cggccaccca acaagtgcat atggatgtgg
    38521 taaacctctt gtccgccatg gcggttggta ttcatgatca gacgatagcc atcttcggca
    38581 ataccttctt gctcagcaat ttttgccgct acggtgatca tgcgtcccag cgcctgctca
    38641 tgctcagctg acacgtcgtt cacagtcgga atgaggatat tcggaatgat cagaatatgc
    38701 gttggcgctt gcggcgaaat atcgcgaaac gccgttacca gatcatcctg gtagacgata
    38761 tcggagggga tctcacgacg aataattttg ctgaatatag tttcttctgc cacgacattt
    38821 tcctttttca aatagccatg cgttgcgcca tgctacgccg cgccacactg cgagtataga
    38881 gtatggtcga gttaaacctg ctctttcaac cttaactcac gatttcttaa gcaaaaaatc
    38941 gttttgctgc cgtatatatc gccattattc ccatttctgc ctgcgaatgc gctgaaaaga
    39001 cattctctgt ttggtttaca cgacaaatat gaatgcgtat atttctcatt tgcatttgca
    39061 agcaaaatta ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga
    39121 cgacaacata aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat
    39181 atcaagccat caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt
    39241 ctgccgcttt tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag
    39301 ctccagatga tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc
    39361 agatgactca acgtgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag
    39421 atcagcagtt acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc
    39481 aggcggattc cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg
    39541 ttgatggtat ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata
    39601 tggcactgtt tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg
    39661 gtaatccatc tgcggcaatt aatatggttc gaaaacacgc gaccagtcgt gaatttaaag
    39721 gcgatgtctc ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa
    39781 gcccactcac cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg
    39841 actcatggct ggaccgctac aatagtgaaa agaccttctt ctcgggcatt gtcgatgctg
    39901 atttaggcga tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata
    39961 gcccaaccta gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc
    40021 gcgcacgcag taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta
    40081 tgaccctgaa gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg
    40141 aagtcgaatt tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta
    40201 tgctggttgg gccatacagt aattatggac ctggctttga tcatgtcggc ggcaccggtt
    40261 ggaacagtgg caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat
    40321 tgtttggtcg tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt
    40381 acttcagttc atgggccaac atcttcccgg atgaaattgg cagtttctac aactttaatg
    40441 gcaatttccc acaaaccgac tggtcaccac agagcctggc gcaggacgat accacacata
    40501 tgaaatcgtt atatgctgcc actcgtgtca cccttgccga tccgctgcat ctgatcctcg
    40561 gcgcacgtta taccaactgg cgggttgata cgctgactta cagcatggag aaaaaccaca
    40621 ccacgcctta cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct
    40681 atacctctat tttccagccg caaaatgatc gtgacagttc aggcaaatat ctggctccaa
    40741 tcaccggtaa caactacgag ctgggtctga aatcggactg gatgaatagc cgtctgacca
    40801 ccacgttagc catcttccgt attgagcagg ataatgtcgc tcagtccacc ggtacaccta
    40861 tccccggcag caacggcgaa accgcctata aagcggtgga tgggacagtc agtaaagggg
    40921 tggaatttga actcaacggc gcaattaccg acaactggca gctgacattt ggcgcaacgc
    40981 gctatcttgc agaggataac gaaggaaacg ccgttaatcc taatctgcca cgcaccacgg
    41041 ttaaaatgtt caccagctat cggttgcctg tcatgccaga gttgacagtc ggcggtggtg
    41101 ttaactggca aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc ttccgcgccg
    41161 agcaaggtag ctacgcgctg gtggatctct tcacccgcta ccaggtgacg aaaaacttct
    41221 cgttacaggg gaacgtcaat aacctgttcg acaaaaccta cgataccaac atggaaggtt
    41281 ctatcgtcta cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa ttctgaataa
    41341 cacctgtaaa aaaggcagcc atctggctgc cttagtctcc ccaacgtctt acggattagt
    41401 ggttacggat gtactcatcc atctcggttt tcaggttatc ggatttagta ccgaaaatcg
    41461 cctgaacacc agaaccagca accactacgc ccgctgcgcc cagtttcttc aggccagcct
    41521 gatccacttt agacacatca gcaacgctga cgcgcagacg ggtaatacat gcgtcgaggt
    41581 tagtaatgtt ttctttacca ccaaatgcag caaccagagc cggtgccatt tcgctggtac
    41641 ctgttgcttt tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga tccagtgctt
    41701 taatcagcac gcggaagatg gtgtagtaaa caatcgcata accgataccg acgatcggga
    41761 acagccacag tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga ccgtgcgaga
    41821 acgacgtacc gtcacgcatt cccagaagaa tacagattgg gaatgccagg cctgccagaa
    41881 tcgcgtggat gatgtacagg atcggcgcaa cgaacatgaa gaagaactcg atcggctcgg
    41941 tgataccggt caggaacgag gtcagcgccg cggagatcat aataccgccc actttcgcgc
    42001 ggttttctgg tttagcagag tgccagatag caattgcggc agctggcaga ccgtacattt
    42061 tgaacaggaa gccaccagac agtttacccg cagtcgggtc acccgccata taacgcggaa
    42121 tgtcgccgtg gaaaacctga cctgctgcgt tggtgtattc accaatctgc atctggaaag
    42181 gtacgttcca gatgtggtgc agaccaaacg gtaccagaca acgttcgatg aaaccgtaaa
    42241 tgccaaacgc aactaccggg ttctggtaag cagcccactg agagaaggtc tggattgcag
    42301 aaccaatcgg cggccaaatg aaggacagca caacgccagt aaagatggca gccaggccag
    42361 aaatgatcgg cacaaagcgt ttaccggcaa agaagccaag atactcaggc agcttaatac
    42421 ggtagaaacg gttaaacatg tacgctgcaa tcgcaccgga gataatccct ccgagtacgc
    42481 cagtatccgc caagtgttta gaggcgattt cttcagcagg taaatgcagt accagtggcg
    42541 caaccacggc catggtttta accatgatgc cataggcaac aactgcggcc agcgcggata
    42601 cgccatcgtt attggtaaag ccgagggcga caccgatcgc aaaaatcagt ggcatgtttg
    42661 caaagacgga accgcctgct tctgccataa catgcgatac aacggcgggc agccagctga
    42721 aattcgcgga accgacgccc agcagaatac ctgcgatagg tagtacggat accggcagca
    42781 tcagcgattt accgaccttt tgcaggttag caaatgcatt cttaaacata attgagagtg
    42841 ctcctgagta taggtgcttt ttttacgttc tcacgcgtgg caagggggga gagcctcgcc
    42901 gtgtacaggg catctaagcg ccctttattt attacacaga gtaaaataat tcagtgccaa
    42961 tttgtttgac ggctatcacg tttcaacttc agatcattgg agaagttgca cattttgaca
    43021 aaacatatgt cacaatattt caaacgcggt tattcaccgc ccaattttcg gcggtgaact
    43081 ttactcgttt tagccattaa ttacgagctt taaaaaaact cattcaacgg atggattgaa
    43141 ggcgggaagc gtcgatgtga aacagacggg cgaagttatc ggtggttacc tgcgccagtt
    43201 cttcaacggc aacacctttc aacacagcca tgtattctgc aacgtcacga accatcgcag
    43261 gttgattctc ttttcctcga tgcggtaccg gcgcaaggta aggtgagtca gtttccacca
    43321 gtaatcgatc cagggggaca taacgcgcag catcgcacag ttgctccgca ttacggaagg
    43381 tcacaatgcc ggaaaaggag atgtaaaatc cgagatccag taatttaccc gccgtttctc
    43441 tgtcctctgt aaaacagtgt agtacgccac cgcaatccgt cactttttct tcgcgcagaa
    43501 ttgccagcgt atcggcacgg gcgtcacggg tatggacgat aaccggcttg ttcagttcac
    43561 gaccaatctg gatatgatgg atgaaggact cttgctgacg tactttagtt tccggtgtgt
    43621 aataataatc cagcccggtt tcacccagcg ctacaacacc ctcttctgcc gccagacggc
    43681 gtaaatcttc tacatcgtag ggatcattct ggttgagtgg atgcacgcca caagaaaata
    43741 cgacgttgtc acgttcgcct accagatccc gcatatgtaa ataacccggt aatgttgtgg
    43801 cgactgccag acaaaatttc acatcgcgtg cggcggcttt cgccagaacg tcatccacgt
    43861 ccttatgcaa agattcataa tccagaccat cgagatggca gtgtgagtcg actaaaaaca
    43921 tgatgtctct cttaaagatg aggaaccggt agcacaacgc ccggttgcag gtaatgctca
    43981 atacgcagta aaagatcggt gatgagaagc tcgcggttga tgcctgtgac agacattaac
    44041 tgttcacgaa tgtggcaaac atcccccagt atagcctgca ggcgcgaggg agaaagatgg
    44101 tttgccagtt cggcgaccag gcccggcaca tcaacattgg tcaccagcgc agcgccatga
    44161 tggcgtttta gcgcatccat cagcaacgtt gccacccagt gtaaacgcgc cggagcttgt
    44221 tcatgattaa gggccgccag cagcgaatac cagtcgcccg atggcacgct atatgccaac
    44281 gcctgacaca atgtttcacg agcctgccag ttatctccct gaaacaacgc cagtgccgcg
    44341 ccaggcgaac cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga cattgtcact
    44401 tcgcgtgaaa gccaagtcac ggcgtactgt tctggaggcg gcgcaaggta atgtaaccga
    44461 caacgactac gtaatgttgc cagtaaacgt tcaggctcgc gggtagccag gaaaaaccag
    44521 gtttctgctg gtggctcttc aagcgttttc agcaatgcgt tagccgcggc gtcggtcagt
    44581 aaggcagcat cggttaccca gacaactttc gcaccaccta ggcgtgcgtg ctcattcagc
    44641 ttttcggtga cctcacgtac cgcatcaatg cccagcgtat ttttcccttt ttcgggagcc
    44701 agggtgtagt aatcgggatg cgtgccagcc tgcatcaact gacatccacg acagtgaccg
    44761 caacttttgt ggccctgcgg ttgttggcag agtaaataac ggctcagggc gtagattaaa
    44821 gcatcatcgc ccatgcccgg taacgcctga atgagtagcg catggtgacc tcttccggcc
    44881 tgatagctgg ctaccagttt ttcgaaatca ggtcgtaacc atggatacca tctcatgcgt
    44941 ccaactcctt cacccagtgg gtcacggtag tgcggattgc atccatcacg gcctccagcg
    45001 gctgggtggc atcaatggta tgaatacttt tatcttgtgc agccagttcc agatagcggg
    45061 cgcgggtgcg attaaagaaa tcgaaagact cttgctcaat acgatccagc tcgccgcgcg
    45121 cacgcgcgcg ttttaagcca acttccgggg taacatcgag atagagcgtt aagtcggggc
    45181 gaaaatcccc gagaacagca tcacgcagtg ttgccagcat atgttggtca ataccacgtc
    45241 ctccgccctg atacgcctga gtggagagat cgtggcgatc gccaatcacc caggtgccgt
    45301 tagccagcgc aggtttgatg accgtttcta ccagttgaac gcgcgcggca taaaacatca
    45361 gaacttcggc tttatcggta atgacttcat cgcctaccga tttgatatcc agcaccaggc
    45421 ttcttaactt ttcggcaagt tgcgtaccgc caggttcccg agtgaaaacc atgtcgcgga
    45481 tacccagttg ctcgagcgtc tcaaccacca cattacgcgc ggtagttttg cctgcgcctt
    45541 ccagcccctc aatgacgata tacttactgc gcattttttt ccttaagcac tttcagataa
    45601 tcctgcacag acttgttatg actggcaaga ttggtattaa acgtgtgacc acctttacca
    45661 tcggccacaa aatagagata cggcgttttt gccggatgcg cagcagcctt cagcgaatcc
    45721 gcccccggcg tagctatcgc acccggcggc aaaccggtaa tggtataggt gttatacgct
    45781 gtcggcgttt ccaggtctgc acgagaaagt ttgccattat aacgctctcc catcccgtaa
    45841 atcacggttg ggtcggtctg caggcgcata ccaatgcgta aacggttgat aaataccgag
    45901 gcaacctgat cgcgttcact ggcaacggcg gtttcttttt cgataattga tgccatcgtc
    45961 accagctgat ttttatcttt ataaggcaga ccgtccgcac gcccttccca ggcgctatcg
    46021 accgctttca ccattttctt gtgagctcgc ttgagtaacg cgacatcggt ggtattggcg
    46081 gtatacatca aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt ttccagttca
    46141 agtgcctgcg ctacggtggc gtacttatcg tcactcagcg tgtgcttgat atacggagcc
    46201 tcacgcaatt gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg cagcgggaac
    46261 tgtgcttctt taccgctttc caacaatttc agcatctcgc gcacggtcat ctgcggtgta
    46321 aagcggtaag tcccggcttt aaagtgagaa agatccggtt cgatacgcag cagccattga
    46381 aaaacccgtg ggcgattgat gatcttatca gcataaagct gttcaccgag cgccagacgt
    46441 ccggtccctg gcttcagggt aaatatcgtc tcttctttga taagcaattt gctgtcggca
    46501 agatggcgaa ccttccagac gcccacacca gcggcgatac ccagtaccac cagcaataac
    46561 aagataatca ataacacttt tttcatgact aattcgggcg ctcacaaagt ggggctaaat
    46621 attcatataa cgttgctgac gaaaaggtga catcgccaca ggcacgtacg ggcatcactg
    46681 gcattaacgc attacaaata accatctcat ctgcctgcaa cgcctcctcc agcgaggctt
    46741 gcacttcgac aagctgataa gaggattgtg ccagtaaacg gatacagaat tgtcgcataa
    46801 tgccgttaac acctgcctga tccagtcgcg gcgtataaac tacattgccc ttccgccaga
    46861 acaaattagc cgcacagcat tccgtaaccc acccttcgct gtcaaggacc agcgcctcat
    46921 cagcgtttgt ctgctcaaga tgagagcgaa tcaatacttg ctcaagccga ttaagatgtt
    46981 taatacctgc aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc aacgtcatcc
    47041 cttcgttacg caaacggtcg taatgtgcag gataagccgt aacggagaga atccgcgttg
    47101 ctggtccgct gttcaatgtg ctgtaccctc gcccgccact accgcgactg atcacgactt
    47161 tcagcacacc attttgctgt tctgctgcca gcgttttcat ctcttgttca agctgaggcc
    47221 agaagtcaca ggaaatcatc aaccgctgac aagcatcctg tagtcgctgg atatgcgccg
    47281 ataacaaact gactttaccg tcgataactc tggcggtggt aaaacaacca tcaccaaact
    47341 gcgttgcccg atcgcttact gccagcgatt cctgcttata accgttaatt aagaacatac
    47401 cggctcctta tcagagaatt gttagtgtgg cagcatgttc actacggaac aagtcggaat
    47461 aaaagctaag aaaaaaggcc cgcaagcgga ccttttatat gggtggaaaa tgacaactta
    47521 gatcttttta aagatcaaag aaccattagt gccaccgaag ccgaaggagt tacacagcgt
    47581 gtattccatt ccgctgacct gacgtgcttc atgcggtacg aaatccagat cgcaaccttc
    47641 atccgggtta tccaggttga tggttggcgg aacagcctga tcgcgcagcg ccaggatgga
    47701 gtagatagat tctactgcac ccgccgcacc taacaggtga ccggtcatag atttcgtgga
    47761 gcttaccaac acacggcttg cagcttcacc gaagatggtt ttcaccgcct gcgcttcagc
    47821 tttatcgcca gccggcgtag aagtaccgtg cgcgttaacg tagccaatct gactcgcttc
    47881 aatgcctgca tcacgcagag catttgccat tgccagagct gcgcctgcgc cattttctgg
    47941 cggtgacgtc atatgataag catcgctgct cataccaaag ccgacgagtt cagcgtaaat
    48001 tttcgcaccg cgttttttcg cgtgttcgta ctcttcaagt accagcatac cggcaccatc
    48061 gcccagtacg aagccatcac gctctttatc ccacgggcgg ctcgccgctt gcgggttatc
    48121 attgcgggta gataatgcac gtgccgcgcc aaaaccacca acgcccagcg gcgtactggc
    48181 tttctctgcg ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc
    48241 atggccaatg ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc ttgggccacg
    48301 caggccatac atgatagtca gatgacctgc caccatgttc acaatcgttg acggaacgaa
    48361 gaatgggctg atcttacgtg gaccaccgtt catcagagat gtgtggtttt cttcgatcag
    48421 tccgaggccg ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt tctcttccgt
    48481 tatttcaagg ccagaatcct gcatggcctg aacgccagcg acaattccat attgaatgaa
    48541 ggcatccatc ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa aatcctttac
    48601 taagccagca aatttcgttg cataggcgct agtatcgaaa tggtcgatta ggctgatgcc
    48661 actctgaccg gcaagcagag ctttccaggt agactctacg gtattgccga caggagacaa
    48721 catgcccagt ccggtcacaa ctacacgacg cttagacacg tttgtcctcc aaggagggaa
    48781 aaatgattct agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg cctggagatg
    48841 ttcacttacg cctggtggcc gttgatgtaa tcaatggcag cctgaacggt ggtgattttc
    48901 tcagcttctt cgtccggaat ctcagtatca aactcttctt ccagagccat taccagctca
    48961 acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag aagcattgtt ggtaacttct
    49021 tcctgcttaa cgcccagctg ttcgccgata attttcttaa cgcgttcttc gatagtgctc
    49081 atactcttaa atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt gtataaaatg
    49141 ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg attttacgtt attttgaggc
    49201 cttacccccc aataacgcaa atcattttgc actaattatt actcataacc acgcagactg
    49261 cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca tgtacatccc gccgttcaca
    49321 tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc aaccgcgttg
    49381 gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat acccgcacgc
    49441 tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc aacaacgttt
    49501 acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc gatcaagccc
    49561 gctttcgccg cagcgtagtt ggcctgaccg ccatttccca tggtaccaac cacagaaccg
    49621 atagtgataa tacgaccatg acgctttttc atcatagcgc gcattaccgc ttttgacaga
    49681 cggaaaacag atgaaaggtt agtttcgata atatcgttcc actcttcatc tttcattcgc
    49741 attaacaggt tatcacgagt gataccggca ttattgacca ggatatccac ttcaccaaat
    49801 tctgcgcgaa ttttttccag aacagattcg atagatgccg ggtcggtcac attcaacatc
    49861 agacctttgc cgttggcacc taaataatca ctgatcgcct gagagccatt ttcactggtc
    49921 gcagtgccaa taactttcgc gccacgggct gcgagcgttt cagcaattgc gcggccaatt
    49981 ccgcggcttg caccggttac cagtgcgatt tttccttcaa aattcatgat tttcctcttt
    50041 taaagctcga gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc ggtcagggtg
    50101 tcgacaatgc gtttcgtcag gccagtaagc accttgcccg ggccgacttc atagagatgt
    50161 tctacgcctt gcgctgccat gtactcaaca gacttcgtcc actgaaccgg gttatacaac
    50221 tgacgtacca gtgcgtcacg gatggcatca ccattggttt cgcatttcac atcaacgtta
    50281 ttcacaacag aaactgttgg tgcgttaaag gtgattttcg ctaattctac tgccagtttg
    50341 tcggctgctg gtttcatcag cgcacagtga gacggtacgc tcactggtaa cggcagcgcg
    50401 cgttttgcgc cagcagcttt acaggcagcg ccagcacgct caaccgcttc tttatgaccg
    50461 gcaataacca cctgtcccgg agagttaaag tttaccggag aaacgacctg accttctgca
    50521 gcttcttcac acgctttcgc aatagacgca tcatccagac cgatgattgc cgccatagcg
    50581 cccgtgcctt ccggtacggc ttcttgcatg aacttaccgc gcatctcaac caaacgcacc
    50641 gcatcagcga aatcaatcac gccagcgcaa accagcgcgg agtattcccc caggctgtga
    50701 ccggccatca ttgccggtgc tttaccgccc tgctgctgcc atacgcgata cagcgcaaca
    50761 gatgcagtca acagcgctgg ctgggtttgc caggttttat tcagttcttc agctggcccc
    50821 tgctgggtca gcgcccacag gtcgtagccc agcgccgcag aagcttcagc aaacgtttct
    50881 tcgacaattg gatagctcgc cgccatatca gccagcattc caacggtttg agaaccctgt
    50941 ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa acgaaccagc
    51001 gcggagcccc aagtgaatcc accgccaaag gcttcaagca gaaccaactg ccccggctta
    51061 atgcgcccgt cgcgtacagc ttcatccagc gcgcacggga cagaggccgc agaggtatta
    51121 ccgtggcgat ccagcgtcac cacgacattg tccatcgaca taccgagttt tttcgccgtt
    51181 gcactgataa tacgcaggtt agcctgatgc ggaaccagcc agtccagttg agaacggtca
    51241 agattattcg ccgccagcgt ctcatcaacg atgtgcgcca gttccgttac cgcaaccttg
    51301 aagacttcgt tgcccgccat cgtcagatga attgaattct ctggattcac gcggtcggcg
    51361 tttggcagcg tcagcaattc accataacta ccgtcggcat gcagatgggt ggaaatgatt
    51421 cccggctctt cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa aataataata
    51481 gtcccacgat ctgttggatc gcaggtgcgc gccagtacat cggaaccgac gaccagagca
    51541 tacttcaccg ccccagattt cacgtattga tcggctacgc ttaatgcata ggtgaaacct
    51601 gcgcaggctg ctgcaacgtc aaatgccggg caacctttaa tgcccaacat gctttgaatc
    51661 tgacaagctg cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac gatcaggcca
    51721 atctggtctt tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc aaagcccatg
    51781 gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt acgagtgaca
    51841 atccactcgt cagaggtgtc caccattttt tccaaatcgg cgtttgtccg cacttgttcg
    51901 ggcagatagc tgccagtacc aataatcttc gtatacatgt acgctcagtc acttttcggt
    51961 tatataccgt cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc gcagagttcc
    52021 gcttttgcca ccgtccagca gctcaaaacc agctgggtat acagattcca ggcgagcggc
    52081 aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg cgaccgcaaa
    52141 agctcgctga ttggctgcac catgactttt tatcaccgtg ccgcgcaatc ctaacagaca
    52201 ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc tcttttgtag
    52261 ccaacgcttt aataacagta gccaccacga ccgttttttc ccttcaccct gagatttcag
    52321 cagagaaagg aacatcctga caacaccttc catcgtcttt aacgtgacat ttcctgtaaa
    52381 gccgtcacaa accagcacat ctgtcttgcc agttaacaac tcattggctt caagatagcc
    52441 gatataattg atagaaggga ttgttttaag caccgctgag gcatcccgaa tactgtcaag
    52501 accctttact tcttcttcac caatattgag caacgccacg cgaggattgg gaatttccac
    52561 cacctcttca gccagaactg agcccataat ggcaaattgc accaacattg tgctgtcaca
    52621 atcgacgttg gcccctaaat cgaggaccac cgttttgccc ttttgctgat gtggtaatac
    52681 cgtcaccagc gccggacgct caatcccctc caggggcttg agtaataatt ttgccagccc
    52741 catcagtgcc ccggtattac cggcactgac acaggcttgc gctcgacctt ctttcaccag
    52801 ctccagggcc atacgcattg aactcccacg actggcgcgg atagcttgcg aaggccgggc
    52861 atcactggcg ataactgact gcgcaggaat aatctgcaga cgcgaacgtt gttcaaagtc
    52921 agctttagca agtaatggcg tgatggcgtc gggattgccg actaaaagaa gagtgagttg
    52981 cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc acggaagggc caaaatcccc
    53041 tcccatgaca tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc ccggtttggt
    53101 ctttccccag ttgcctgggg aaaatcctca ctaagcttca tcacgcagac gcgtgattac
    53161 ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg acgcaggtgt
    53221 ttttcaccag aagttttgtc tacagacagg ctggtgactg cggtcagcgc gtcatgggaa
    53281 cgacgcatgc cacgtttgga acgggttggt ttattctgtt gtacggccat ggaccttact
    53341 cctcaattac ggtttatccc cgatccaacg gggagcacca attacttacg ctttaagctg
    53401 gctaatacgg caaatgggtt tggcttttgc acttcttcag gcagttcacc aaagaccata
    53461 tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg gaactaccgg caaggcgagg
    53521 atgatttcat cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt aacctcaatc
    53581 ggttcatacg cttccggcag tgcttcagcc tgttcgtctg aacgcacagg actaaaacaa
    53641 tacgttgtgt agacctgatg agtaaacggc ttcccgcaac gctgacactc gagcgttacc
    53701 gtcaccttcg catcgccgtt taacactgcg agacgttggt tatcgatagc gaacgacatg
    53761 gagcattcca catcactgtc cacactgact acggattcgg cgacgcgctc aacctgatca
    53821 ggggtataga taccctggta atcaaggcgt ttttgagccg tacgaaccgg atcgagagtc
    53881 agggggaatt ttaccttttg catagggcgc gcatattaac tttgtaacgt catagagtca
    53941 aagaaaaagg cagcctgtgg ttgccttttg ccaataattc gcacacattg cgggttactg
    54001 ctttattttc gttcagtgct gataacgaac atcaacctgc aaaacgaagc gcatagttta
    54061 aaatggctat catcaatacg ctatatctga tggaaaaaaa tatgcctaaa cttattttag
    54121 cctccacctc gccatggcgt cgcgctcttc tggaaaaact gcaaatctct tttgaatgtg
    54181 cagcacctga ggtcgacgaa accccacgca gcgacgaatc accgcgacag ttggtgcttc
    54241 gactggcaca agaaaaagcg caatctctgg cgtcacgtta tccggatcat ttaattattg
    54301 gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta acggaagaaa
    54361 atgcccgtct gcaattacgc aaagccagcg gcaatatcgt caccttctat actggactgg
    54421 cgttgtttaa ttcggcgaat gggcatctac aaacagaagt ggagcctttt gacgtccatt
    54481 tccgtcacct aagcgaggcg gagattgata attacgtgcg taaggagcat cccctgcact
    54541 gcgcgggtag ctttaagagt gaaggatttg gcattacgct gtttgagcgt ttagagggac
    54601 gtgaccctaa cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg ttacgtcggg
    54661 aagggaaaaa cccgctgatg ggatagttgt ttgcgccgga tgcggttacg tcgcatccgg
    54721 caatcggtgc aggcggcctg atgcggcgta aacgccttat caggccaacc gcccattatc
    54781 aggcttatat gcttgtatta gcgcgcgtta cgcagttttt gcaaacaacg cttcaaacct
    54841 tcatccatcg gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt aaacttcaac
    54901 gctgcggcgt gcaggaacag acgatttaat cccgtgcctg cttcagtgag ctgtctgtca
    54961 aattcacggt caccgtagcg atcgtcaaag gcaatcggat gacccgcata ctgtgtatgc
    55021 acacggatct gatgagtacg tccggttacc gggctacaac gcaccagggt ggcaaatgca
    55081 tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc cttcctgact gacacgcacg
    55141 atacgttcgc cgctttgcag aatatttttc aataacggcg cttgaacgct cttcacatgc
    55201 gactgccact gaccgcgcac cagcgccagg taatcttttt gcattccttt ttcacgtaat
    55261 tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac acctgaagtg
    55321 tcccggtcga gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa
    55381 ccttcgataa cgccgaagct taaaccactg ccgccatgta ccgccgtacc ggaaggttta
    55441 ttcagcacca ggatatgatc atcttcatat aagatgacgt ccgccagcgc cgccaccttt
    55501 tgcagatgtg gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg tgggatacgc
    55561 acctcatcac ccgcttcgag tttatattca ggcttaatac gttttttgtt cacccgcact
    55621 tcacctttac gcaaaatacg gtaaatcata ctttttggta cgcctttcaa ttgggtacgc
    55681 aaaaagttat cgatacgttg ccccgcttcg tcagcggtga tagcaacaat ttttacggat
    55741 ggagtctctg ttttcatggt gggcgattct aattagccaa caggattcgc gccactcatt
    55801 tttctatgct tatatttagt tttgcacctt attacttcac tacgtgatca ctttattgat
    55861 ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg
    55921 gtaaaagtca tcttgctata acaaggcttg cagtggaata atgaggccgt ttccgtgtcc
    55981 atccttgtta aaacaagaaa ttttacggaa taacccattt tgcccgaccg atcatccacg
    56041 cagcaatggc gtaagacgta ttgatctttc aggcagttag cgggctgcgg gttgcagtcc
    56101 ttaccggtag atggaaatat ttctggagag taatacccag tctgtttctt tgataattgc
    56161 gctgtttttc cgcatgaaaa acgggcaacc gacactctgc gcctctttga gctgacgata
    56221 accgtgaggt tggcgacgcg actagacacg aggccatcgg ttcacacccg gaaaggcgtt
    56281 actttgcccg cagcttagtc gtcaatgtaa gaataatgag taagttacga tgaaaagaat
    56341 gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct
    56401 gtatgaccta gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca tctacaaagg
    56461 taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg gcgctgaacg
    56521 tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta actacagtgc
    56581 tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat
    56641 cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca gtctggcggg
    56701 tagctatctg gttctgatgc caaataaccc gcgcgcaggt ggcatttctc gccgtatcga
    56761 aggcgatgac cgtaccgaat taaaagaagc actggcaagc cttgaactgc cggaaggcat
    56821 ggggcttatc gtgcgcaccg ctggcgtagg caaatctgct gaggcgctgc aatgggattt
    56881 aagcttccgc ctgaaacact gggaagccat caaaaaagcc gctgaaagcc gcccggctcc
    56941 gttcctgatt catcaggaga gcaacgtaat cgttcgcgcc ttccgcgatt atttacgtca
    57001 ggatatcggc gaaatcctta tcgataaccc gaaagtcctc gaactggcac gtcagcatat
    57061 cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg gcgagatccc
    57121 gctgttcagc cactaccaga tcgagtcgca gatcgagtcc gccttccagc gtgaagtccg
    57181 tctgccatcc ggtggttcca ttgttatcga cagcaccgaa gcgttaacag ccatcgacat
    57241 caactccgca cgcgcgaccc gcggcggcga tatcgaagaa actgcgttta acaccaacct
    57301 cgaagccgcc gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg gcctgattgt
    57361 tatcgacttc atcgacatga cgccagtacg ccaccagcgt gcggtagaaa accgtctgcg
    57421 tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc gctttggcct
    57481 gctggaaatg tcccgccagc gcctgagccc atcactgggt gaatccagcc atcacgtctg
    57541 cccgcgctgc tccggtaccg gtaccgtgcg tgacaacgaa tcgctgtcgc tctctattct
    57601 gcgtttgatc gaagaagaag cgctgaaaga gaacacccag gaagttcacg ccattgttcc
    57661 tgtgccaatc gcttcttatc tgctgaatga aaaacgttct gcggtgaatg cgattgaaac
    57721 gcgtcaggac ggtgttcgct gcgtgattgt gccaaacgat cagatggaaa ccccgcacta
    57781 ccacgtgttg cgcgtgcgca aaggggaaga aacgccgacc ttaagctaca tgctgccgaa
    57841 gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc
    57901 tgaacaacct gcactggcaa cctttgccat gccggatgtg ccgccagcgc caaccccagc
    57961 tgaacctgcc gcgactgtcg tagccccagc accgaaagct gcaacggcaa caccagcagc
    58021 tcctgcacaa cctgggctgt taagccgctt cttcggcgca ctgaaagcgc tgttcagcgg
    58081 tggtgaagaa gccaaaccga ccgagcaacc aacaccgaaa gcagaagcga aaccggaacg
    58141 tcaacaggat cgtcgcaaac ctcgtcagag caaccgccgt gaccgtaatg agcgccgcga
    58201 cacccgtagt gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg
    58261 ccaggcacag cagcaaactg ccgagacgcg tgagagccgt cagcaggctg aggtaacgga
    58321 aaaagcacgt accaccgacg agcagcaagc gccgcgtcgt gaacgtagcc gccgccgtaa
    58381 cgatgataaa cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag agcaatctgt
    58441 tcaggaaacc gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca
    58501 gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag tcgcaccggt
    58561 ggttgaagaa actgccgctg ccgaaccaat tgttcaggaa gctccagctc cacgcacaga
    58621 actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc aagaagagaa
    58681 caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca
    58741 cctgcgcgtg agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc caacccagtc
    58801 gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca aagtctggat
    58861 ccgctatcca attgtacgtc cgcaagatgt acaagttgaa gagcagcgcg aacaggaaga
    58921 agtacaagtg cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg aacctgttgt
    58981 tagcgcgcca gttgttgaag aaatggctga agtcgtagaa gcccccgtac cggttgccga
    59041 accgcaaccg gaagtggttg aaacgacgca tcctgaagtg attgccgccg cggtaactga
    59101 acagccgcag gtgattaccg agtctgatgt tgccgtagcc caggaagtcg cagaacacgc
    59161 agaaccggtg gttgaaccgc aggaagagac ggcagatatt gaagaagttg ccgaaactgc
    59221 tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga
    59281 agtcgcagca gaagttgaaa cggtagccgc ggttgaacca gagatcaccg ttgagcataa
    59341 ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg aggcaccgcg
    59401 tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg caggtggtca
    59461 tacggcaacg catcatgcct ctgccgctcc tgcgcgtccg caacctgttg agtaataatt
    59521 agctcaaagt aatcaagccc tggtaactgc cagggctttt ttatttcatg tttgaatcaa
    59581 catattaaaa ttcatcaaat ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa
    59641 atgcccgccc cggcgatccc agagcgggca gacaaaacat acccagtttc atgatatgtt
    59701 ttaaagcgag cttatttgtt gagctggaag agcgacaatc cctgcatatc ggtaaatgct
    59761 ttatacgatg cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt tgcattccag
    59821 tccacatcaa ccagatcgct catctgctgc gtttgcccta aagcgcgatc gctacctaag
    59881 gaatccagcg actccagttt gttcagctgc gtgcctaatt ccgtgcgaac ggtaaggaca
    59941 ttattcagcg agtttttcag tccacggttg gttttatcca atgccgcagc ggcgatttct
    60001 ttatccgctt cgctatccgc gaccggcgtt ttcagggctg cgatggcgct atccagcatg
    60061 gcaaaaagat tggtttcaga agcgctaccg tctggttccg ctaccgcgtt actggtaata
    60121 ctgtcgaaaa ttttgtcgcc cgtatgtcct atcaccatcg aacgcgaagc atcgacctgt
    60181 tgcttaatac tttctgttcc gccgacataa tcaccgttag cctcactaaa cggcgcagtc
    60241 tctgttttat aaccggcaaa aatgtagcgc ccattaccgt cagtggtgtt cgccagattc
    60301 agcaactggt cacgaagccc ctgaatatcc gtagccagcg aggcccggtc atcgtcactc
    60361 aaggtgccat tgctggcgta gacaattttt tcctgagcat tctggatagc agtggtgacc
    60421 tggctaagta cactctcttc cagtgacact ttttgagtgg cgaaagtacg tgccagcgtg
    60481 tactggctgt tttgcgcctg tgcctgggag agaacaacag cttgtgatgc agcaatggga
    60541 tcgtctgaag ggttaacgac tcgcttaccc gtcgacatct gctcgccgta cttcatccat
    60601 tctgcctgag aattggtgat gccgcgcatg ttttgctggt acatcatctg ggtactgaaa
    60661 cgcattttat ctccccttag cgaatgttaa tcagcgcatc aaaaatcgcg tttgccgtct
    60721 gcagaacctg cgcattcgcc aggtaatact gctgaaaacg ttgcagattt ccgtattcct
    60781 catcgagatt gacaccggaa atcgactgct gctgattgga aagctgcgtc accacattac
    60841 cttgcgtggt gctactggtt ttcaacgtcg cggttttatt accgatatca ctcactaacg
    60901 aggcataagc gtcgttaaag gatttcgcac cgcccaccgt tttactgttg ctttgcagat
    60961 ccagcagggc ctgaccgttg cggttgtcgc tatcacccgc atcttcttcg ctcgccatcg
    61021 cgattttcgc ttcgtcggtg attaatacat ccatgttgac gatggcgtca cttactggtt
    61081 tcagcgtgaa gctgtcgtta acggcaggcg ttcctgtaaa cgtcaactcc agaccatcaa
    61141 atgccacttt accgttggca tctggtgtcg ccgtaaaagt ggtattgctg gcaaggcggg
    61201 tgacctgcca ctgattatta tcgaacgaga ttttgtaatc tgtcgccagt accacggagg
    61261 catcagttac cgtggcaccg atcgcaacgt cacctttgtt tttcgtgttt tgctgaaccg
    61321 cgggattacc gatagtaaag aaatcttcac cagcatcgcc gttagcatca aatccggctt
    61381 tgtgttggct gttgaaagcc tcggcaaatg ccagcgccag ttgtccaagc gtattacgcg
    61441 tctggtccag atcctgagaa cggaatgtca gaatgccgcc cagcgacccg gtattcagta
    61501 atttctccgg gatctcaata ttgcctgccg tcccatcaac gtaagcgaca gtcgtacgag
    61561 aagggtcggc gctggaagga actgccgcca gttgccgcgc cgtacttccc tgaaccagtg
    61621 agtaaccatt ggcatcgtga tgttataagt accgccatcc tgaacgctga cttctacgcc
    61681 aacaatctgg tttaattcgc tcaccagttg atcgcgttga tccagcagat tgttaggtga
    61741 cgcccctgcc cccacgcctg tcaggcgcga gatttgatcg ttcaggctgg caatttgttt
    61801 agcgtaattg ttgatctgat caacgctggc acctatcgcg atattgacct gtttgtcctg
    61861 gtcgcgcaga tattgatcgg tggttttaaa ctgattcacc aacccttctg atttcccaat
    61921 cagcgcctgg cgcgccgccg ggtcttccgc gttactcacc agcgtttgca ggctggtgaa
    61981 gaaatcctgc atctgtgttg ccagcgaaga ggtactggtg gagagcatat tgtcgatttt
    62041 cgacatctgc tcatagcggg cggtcaggcc gctactttgc gtctgcacag cacgtaactg
    62101 gttggtaata aacgtatcat actcacgctg cacaccagaa acgtagacgc cattgccaac
    62161 ccagccgcca gcgcccaacg tgctattggc ctgcgccata atagtggttt ggcgggtata
    62221 tccggcaacg ttatagctgg agatattatt acttgccgta tttaacgccg cctgggccgc
    62281 gttcagtccg ctcatggcgt tattgatcaa gctggacatg gaggttcctt ataagccttc
    62341 aattacagag tattatcggc agcgacccgc cggacttgag ttattcagaa cagattatca
    62401 atgttcatgc tgtaggtttt gctcacctgg tcgcttatcg atttcatctg ctggatcatg
    62461 ttggtgagtt tacgggcata gtgaggatcg gtagcatagc ccgcgtcctg taggacctgc
    62521 gccccctgtt ccgcactcgc ggcggtcgtc acggcggcgt agcgcgggtt acgtgttaac
    62581 agcccaacgt aatccgacaa tgcttccaga tacgagctgt agacccgaaa cttcgctttt
    62641 actttcttcg cttcgccgtt ttcatattca gtcgtggtga tttcagtgac ttgccctttc
    62701 cagttgccag aggctttgac accaaacagg ttatagcttg gctcgccgtt ttcgcggcgg
    62761 atttgccgtt gcccccaacc agattcaagc gcagcctgag cgaggatcaa atgatgtggc
    62821 acaccgcttt gctggcttgc cagttgggcg ggcagcgaga gttgcgcgag gaatgctcta
    62881 ctgtcacccg gcagcgaatc atcgtagtta cgtggtacgg ccttttgcac cagctgcgaa
    62941 agcgtctggt tttgataacg caccacggtt tcgagcggga atttcatcgg tgctgctggc
    63001 gtggactcct ctggcaatgg ttgttctggc gtcatctgtt taaccatcat ctctgcaagc
    63061 cccagacctt tgcccgccgt catctgttgg gcaatttgct ggtcatacat actggtatac
    63121 agacgagtgt gctcgctgct gaacaggcca tcttttggta aagcgtcgcg catgcttttc
    63181 aacatcatct gcacgaacat cccttccacc tgacgggcca ccggacggat atttgccgcc
    63241 ggatcttcgc tcgctttcgc ctttagttcg ttgagtgatt gcgcatccca ggccgcactt
    63301 gccagtagtt tgctgtcgct gatcatcaga tgatttccag ttttgcccgc agacatcccg
    63361 cactttgcat tgattgcaaa atagacatca gatccatcgg cgtagcgccc agcgcattga
    63421 gcgcacgcac cacgttattg aggctggcgc tggaacgtac gctttgcagc gaaccgccgc
    63481 tctggcgtaa atcgatctgc gtttgtggag taacaacagt ctgtccacca ccaaacggtg
    63541 tatctggctg gctgacattg gcctgacgat taactgttac tgagagattt ccctgcgcta
    63601 ccgcgcagct gtcgagggtc acttctcgat tcatcaccac cgaaccggtg cgcgagttaa
    63661 tcactacttt agcgtcctgc ggggtgacat taacctgcat attctggata tcggcaagga
    63721 aacggacctg ggaactgttg ccacttggta cgcgcacctg aatagtccgc gcatctaacg
    63781 cggtggcgct gccatatcca cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc
    63841 tgaaatcttc gtcgttaagt tgcaaattaa gggtattccc gacgccaaac tggctgggca
    63901 attcacgttc aataaccgca ccattggtga tccgtccacc gttcagttgg ttaacctgaa
    63961 cactgctacc gcccgcggag gctcctgcgc cgccaaccag aatattgccc tgcgccagcg
    64021 catacacctg actgtcaacg cccttaagcg gtgtcatcaa caacgtacct ccacgcaagc
    64081 ttttggcatt acccatggaa gaaaccacca catcgatggt ttgcccctga cgtccaaacg
    64141 gaggaagtga cactgtcacc attaccacag cgacgttttt tagctgcata ttggtgcccg
    64201 tcggaacggt aattcccagc tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg
    64261 tctgggttgt ctggtcacca gtgccatcca gccccactac caggccatag ccaatcagtg
    64321 agttttgcct taccccctga acactggtga gatcgcgaat acgctcagcc tgagccgccg
    64381 tcgtaaccag tagaagaatt aatgcagaga gaaatttaat caccacagcc tcacttacat
    64441 tggcgacagg ttaaggaaga aacgctgcaa ccagcccata ttttgcgctt cgttaatgta
    64501 gccattgcct acgtattcaa tacgcgcatc cgccacctga gtagacggta cggtattgct
    64561 gccgctgata gtgcgtggat taaccacgcc cgagaagcga ataaattcgg taccctgatt
    64621 aatggcaatc tgtttttcac ccaccacatg caggttgccg ttgaccagta cctggtcaac
    64681 cgtcaccgtc aacgtgccgc taaaggtatt gctggcattg gccccgccct ttccgttgaa
    64741 cgtgttacca ccggaggctt cgacatcggc acgagcgtta ccaaacagcc cctgcaaata
    64801 gcgcggcaca gtatcaaagc caaaattagt tttaccgtca cggctggcat tcgcagagga
    64861 gcttttgctg gcgctgacgt tctcctgcaa cacgatggtc agcgtatcgc caatattgcg
    64921 tggtcgacga tcttcaaaca gcggttgata gccatagtta atcggctgag cagactggaa
    64981 aatagaaccg ttggcgacgg gcgtcggacc gggaaccggt tgtgcactgg tcgccccctg
    65041 caccagcggc gtggagggta tccaggcgca gccggttagt gaaagcacca acaagctgga
    65101 aatggcataa gtatgcgcag cgtttttttg catggctatc atcttcaaaa atcagtaaac
    65161 cggtgaacct gccaccggtt atgccttaga gttgcgtcag tttttgcagc atctgatcgg
    65221 tggtggacac cgctttactg ttgatttcgt aagcgcgttg cacctgaatc atattgacca
    65281 gttcttccgc cacgttgacg ttagacgttt caacataccc ttgatacagc agtcccgcgc
    65341 cgttcaggcc cggcgtgctt tcgttcggtg caccggagga ttgcgtttcg gtgtagaggt
    65401 tttcgccaat gctctccagt ccggtatcat tcataaaggt ggtgagattg agctgcccaa
    65461 cctgaaccgg agctgcctgg ccttgttggg ttacgctgac cacgccatca cgaccgatgg
    65521 tgatacttaa cgcattcgcc ggaatggtga tcgctggctg cacctgaaaa ccaccagccg
    65581 tcaccagctg cccgttctga tccacctgga aagagccgtc acgggtatag gctgatgagc
    65641 catctggcaa cattacctgg aaaaagccct gccctttaat cgcgacatct ttgctgttat
    65701 tggtctgcga cagattgccc tggctgtgta agcgttcagt ggcgaccggg cgtacccccg
    65761 tgccgatttg taatccggag ggtaaggtgg tttgttcgga agactgtgcc cccggctggc
    65821 gaatggtttg ataaagcaga tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac
    65881 tgacgtttgc caggttgttg gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg
    65941 ttttggcgat ccataatgaa ctgatcatat aatgtcctgt agtggattgc tgacaagcag
    66001 agcagaagca cgcgacagtg tgaatttccc ctcaccctaa ccctctcccc aaaggggcga
    66061 gggaatagtc cgcgcgcttt tattgcaatg tcatcaatcg ttcaattaac tcatcgacag
    66121 cagttggttg gcacggcctg cgttatcatc gacgctgctg atcaccttca tctgcatttc
    66181 aaaacgccgc gcgctggcaa tcatgtcgct cattgccgca acggcattga cgttactgcc
    66241 ttccagaacc cccgacatca cacgcaaggt tggatctgcc tgcagtaccg gccctcgcgt
    66301 ggcctgggtt tctgcgctta aacgaaaaat gccgtcgtca ccgcgctgca cttcgctgcc
    66361 cgtggctttc accagtttca gacgccctac tggcgcaact gtatttgccg gatcgcccgg
    66421 attgagcgcc gagattgtgc catcggcagc aatcgtgatt tccgcccctt ccggcacagc
    66481 aattggcccc gcctcgccta tcaccggatg cccctgaatt gtcagttgcc cggtgggatc
    66541 aacctgaatg ctgccattac gcgtataccc ttcgctgccg tcagcggtct gcacggccag
    66601 ccatcctgct gcaacgcaac gtccagcggg cgcgaggtgt aatccatctt gccgggcgtc
    66661 atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg tgggcagtga aagcccttcc
    66721 accggcactg cgcgcaaggc gtttaactgc gcgcgaaaac cgggcgtaga ggcattggcc
    66781 agattactgg cggttaccgc ctgttgattc agcgtctggc tggctgctcc catcgcagta
    66841 taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa cccgcgtgtt
    66901 gaggatctgg tcctgggttt tgatggtctg ggcgttagac tgatagttac gctgggcaac
    66961 gatcatattg accagttctt tactgagatc gacgttggac gcttccagcg caccgttggt
    67021 cagggtgcca aagtttcccg tcccggctgt ccccaacagc gccacgccag aagattgcgt
    67081 cgcagaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa agttcgccag
    67141 tacaatctgc cccagcagtt gggtttgttc gttggaatag ttgccgacaa ccgtaccgtc
    67201 atcattgatt tgataactca ccagatcgcc cggtttgtag ccattctggg tggttgccac
    67261 aatgttgtta gcgccggtat tttgctgcat ggagttgagg aagctcagac taaacgtggc
    67321 gggttctgca ccgttaattg cgccggttgc gatattattc gccatcgcac atgtgccatt
    67381 agcattaaat tccagtgttg tcgctgtctt cgcaatgctg tttggatcac tgctatcctg
    67441 ggtgtagacc tgccagttat tatccccggt cttcacaaaa tagacgctca tgtcatgagc
    67501 attaccctga ctgtcgaaaa cagtcaccga accttttttg ttatagctat ccgcattgct
    67561 ggcgctgaat ggcgtaacag taggaagcgg atcactggaa ttcaggttga tctgcatcga
    67621 cgccgtggtg gtagttttcg ctgccatcag ggtattcggg atcgaaatat tggtcggatt
    67681 cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg taacccgtca gctgtaaacc
    67741 ttgcatattc aacaggttac ggttttcatc cagcttaaat tgtccgttac ggctgtagaa
    67801 caccgaaccg ttgctgtcta ccagacggaa aaaaccgttc tggctgatag caacgtccag
    67861 acctcgcccg gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac cggcaacttt
    67921 tacccccagt cccactttcg aaccggcaaa catatcggca aaagaggccg tgcctgattt
    67981 aaagccgtag gtggcggagt tggcgatatt gttgccaata acatcgaggt tggtggcggc
    68041 agcgtttaat ccgctaaccg cttgagaaaa ggccatgact gactcctgaa atgtgaaggc
    68101 ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg taagtgccga gatccagcgt
    68161 attaccgctg ttgccgcgga tcacaccctg caccagagca aactgcagcg gctgggcaac
    68221 cagttgtgta ccaccgttac tggcgctaat cgctacattg taagaaccgt tcggcgcagt
    68281 gctgccatca gtcaacgtac cgtcccaggt gaaactgtga actccggcgg tcagttcacc
    68341 aatatcaatg gtgcgcacaa ccgcgccatt tttatcggtg atggtggccg taactttgtc
    68401 tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg gtcacagccc cttcttcact
    68461 gccggttccc gcaagaacag ttgtgccggg gatcatcacg ccgtgaccga tcaggttact
    68521 ggcctgtaac gactggctgt tatcaatctg tccggaaata gatccgagcg tggtattgag
    68581 tttttcaatc ccgctgaccg tgctgatttg tgccaattgc gacgtcagct cgttgttttc
    68641 cattggattg gtcgggtcct agtttttcag ctgcgccacc agcaaagtca gaaaactgct
    68701 ttgtaaatct gcggcgttgc tgcccgtgag cgaactactg ctggtggtac tgacgccggt
    68761 atttgtcgga tcggtggtgg ttaccgcaat ggacatagct ttctccttta ttgaccgagc
    68821 gtaagggttt tcagcatcat gcttttcacc gtgttgagca cttcaacatt ggcctgatag
    68881 ctgcgtgacg ccgacatggt gttaaccatc tccccgacga catcaacgtt cggcattttt
    68941 acgtagccct ttgcatctgc cagcggatta cctggttcat aaaccagttt gtccggggcc
    69001 tgactttctt aacatcggca acctttacgc cgcctgtcgc agcacctggt gcagcgttaa
    69061 cctggaatac cacctgtttt gcccgatatg gctgtccatc aggaccggtc acgctatcag
    69121 catttgccag attactggcc gccacgttca ggcgctggga ctgggcagtt aacgccgacc
    69181 cggcgatatc aaaaatattc agcagtgcca tccgttaatt tccgctctgt aaaacgttca
    69241 tcatgccttt gatttgcccg ctcaacgcgc taaggctcat ctggtattgc aggctgttat
    69301 cggcaaactg ggtgacttcc tgacgctggg cgcgcagatt gagcgcctct tgttgaaaac
    69361 gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa ttctgccatc
    69421 agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg gtcgctattt
    69481 atgccgttga tggtcattgc ggacaggtac aactcacgtt gtagaaatgg ctgggggcga
    69541 aaatgctgac aataaaacgt agcgtggcga tcatcgcgat actgttcagt ccgttaagta
    69601 cggcgagcaa tctcacatcg caattgcaca acttttttag cgcccaactc gcgggggtaa
    69661 gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgcca tgcgagcagc
    69721 cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg ttggcacgct
    69781 gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat tatgtggttg
    69841 ccgcgatgcc tattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa ctgaaacgtg
    69901 gacgcctgga taccctgcca ccgcgtacgg tgctggatat caatcaactt gttgatgccg
    69961 ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt cgccaggcat
    70021 ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg tttagcgcca
    70081 acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg gtgcgcatgg
    70141 tatcgggaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt ataaacctgt
    70201 aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat aaaagcgccc
    70261 tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagcct gtaagcaccg
    70321 ttcaaccgcg cgaaaccact gacgcgccgg taacgaacag ccgcctccac cagcaccagt
    70381 gtgacgttaa gcgacgcgca agcaaaactg atgcaacccg gcagcagtga tatcaatctt
    70441 gaacgtgtcg aagcgttaaa actggcgatt cgtaacggtg aactaaaaat ggacaccggc
    70501 aaaattgccg atgcgctgat caacgaagcg cagcaagact tgcagagtaa ctgatcgtat
    70561 gacacgtctt gcagagatcc tcgaccagat gtccgctgtg cttaacgatc tcaaaacggt
    70621 aatggatcat gagcagcaac atctctctat ggggcagatc aacggcagcc agttgcaatg
    70681 gattacagaa caaaaaagct cactgctggc gacgctggat tacctcgaac agttacgcaa
    70741 gaaagaaccc aatacagcaa atagcgttga tattagtcaa cgctggcagg aaattactgt
    70801 gaaaacgcag caactacgcc aaatgaatca acataacggc tggttactgg aaggacagat
    70861 tgagcgcaat caacaggcgc tggaaatgtt gaaaccgcat caggaaccga cgctatatgg
    70921 ggcgaacggt cagacctcaa caccccatcg cggcagtaaa aagatttcga tctgaatatt
    70981 atctccggcc tgcactgcag gccggaatgc attgttacac cgtccggcgg gcaaattctt
    71041 taactttgaa gcccagtacc gccagtgcag cgaagtacgc ggcaatcccc gccagcacga
    71101 ccgccattaa acgcagtaag cgccagggca tggtacccaa tgaccactcc ggcatgatat
    71161 gtaacatacc taaaagcacg ccagacatca ccagtaccgc caccaccaga cgcaacagaa
    71221 acgccatcca gccgggttgc ggagtaaaga ttttctgctt acgcaactgc cagtaaagca
    71281 gcgaagcatt cagacacgcc gccagaccaa tagaaagtga cagcccggca tgtttcaacg
    71341 gaccaataaa cgccaggttc atcaattgcg ttaaaatcag cgtaacgatg gcaattttca
    71401 ctggcgtttt aatgtcttgg cgggaataaa agccaggagc caacactttc actacaatca
    71461 ggccaatcaa acccaccgag taggcaatta acgcccgctg ggtcatcagc gcatcaaacg
    71521 cggtaaattt accgtactgg aacagcgaaa cggttaacgg acctgaaaga atgcccaacg
    71581 caaccgcact cggcagtgcc aacaggaaac aaagacgcaa cccccagtcc atcaaacggt
    71641 tgtattcatc atgattgcca ctggcaaaac ttttcgacag cgacggcagc aaaatggtgc
    71701 caagcgccac gcccagcaca ccggagggaa actccattaa gcggtcggcg taatacatcc
    71761 aagacaccga accggaagca agaaacgagg caaaaatggt gttgatgatt aaggagatct
    71821 ggctcacaga gacgccaagg atcgccggtc ccatctgttt caccacgcgc attgctccag
    71881 catcgtggaa gttaatgcgc ggcaggacca gcatgccgat cttcttcagg tgcggtagct
    71941 gatacaccag ctgcaggatg ccgccgaccg taacagccca cgccagcgcc agcaccggtg
    72001 ggttaaagta cggtgcggca aacagcgcga aaccaatcat gctgatgtta agcagtgttg
    72061 gcgcaaacgc cggaatcgag aagcggttcc acgtattcag aatcgctccc accagcgacg
    72121 ccagggagat cagcaagata tagggaaagg taatctttag taggtggctg gtcagggcaa
    72181 atttgtcagc tgtgtcagcg aagcctggcg cggtcaccat gatcacccac ggtgcggcga
    72241 gcatgccagc gaccgtcaca accgccagcg caagtgtcag caggccagaa acataagaga
    72301 caaagacccg cgtggcgtct tcaccctgct tacttttata ttccgccaga atcggtacaa
    72361 atgcctggga aaatgcccct tcggcaaaga tacggcgtaa caagttagga agtttaaaag
    72421 cgacgaaaaa ggcgtcggtt gccatccctg cgccaaagat tctggcgaca attgcgtctc
    72481 gtgcgaagcc aagcacacgc gaaaacatgg tcatcgagct gacggcggcc agcgatttta
    72541 ataaattcat cggtgttcta atccggaccc acgggcaaaa cgcctgcaag gcaggcgctg
    72601 aaagaggcga atagtctacc tagattatgg tgaattgcta ccgccagatg ttacagggtt
    72661 tattcactca tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag cactgcctat
    72721 tcgccggcgg tttgcggaac tgtctgattt tgcacgcatt caatgaagtg ccgcgcacag
    72781 ccgacaaatc cacgttgctc aagagtgctc tgccaaccag gaatcggttt attcactaca
    72841 ccctgcccgc gctcctcacg ccattcgcgc atatccgtaa tgtcgatgag cgcaccgtca
    72901 gtcacggcct gcacggtttc acgctgactt ccggcacggc gatgcataca agtggtgatc
    72961 tgcaaaggac cggccgaaaa atggtgctcg gcaaacagca tttcgccagc gtcgttagtc
    73021 agtagcgtac cgccatccag agaggctttg ccgcccgaca accacagcgc ggtatccacc
    73081 acatgcagat aatcatccag caacgtgaaa taaagatcgt gtggcccgac gctattgcta
    73141 cgatgtttat ccattcttag cgaggctgcg gtggcgagtt gcgtttttaa ctcaccgtag
    73201 agtggtgcga aacgacggtt aaaaccgacc atcaacgtca gttttttccg cgccgccagt
    73261 tccaccagcc tttcagcatc gcgcagattt tctgccagcg gtttatcgac acagacatgt
    73321 acccccgcat tgagtaacgt actgaccacg tcaaagtggc tggcggtgct ggagtgcaca
    73381 aaaaccgcat cacaactggc ggcaaggctg gataacgaat cggcataagg aatgcgccag
    73441 ctgtcacaaa ttggcagggc tttggcgcgc gtaggcgacc aggctccttg taacgtccag
    73501 tcagacgctg ccgccagcac cggtaaccac gctttttgcg caatgccgcc taatcccact
    73561 acgccgatac gtaatttttt cactttaatc tcccagatgg gccagcaacg aatcaagacg
    73621 ttgtttcagt tctgccactt cgatttccag ggcttcgacg cgggtctgta aatcaccgtc
    73681 aaccatgttc gacatagccg ttaccgccgg ctgatcttca acctcaccac tgaaaagatg
    73741 catgtagcgg ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa aaggaccatc
    73801 ttcgcgattc gccagttgtt ccagcgtcga ctccacttcc gccatatcgc tgaattcata
    73861 cattcgcgcg gcgcggctgc gcagttcacc cagcgtctgg gcaccacgca ataacaacgt
    73921 ggtgattaac gccacttctg cggcgctcag tttcagatcg ccaaattctg aattacaaaa
    73981 acgttgctca tatttggtga cccgattacc aaaaccgctc actgtgcgta gataatgacg
    74041 tttgaccaga ttatccagct gttcctgcac ttcggattca ctcagattca tgaccggttc
    74101 acggttcgtt ttctgattac aggccgtgac tacaccattg actgagagcg ggtattgctc
    74161 cggcgtggtc acctgctttt ccagcaagca accaattacg cgagcttcca gggcagtaag
    74221 ttgatatttc atgagattct ccttagcggc cgggcgtcca gtctggggta gttaatgccg
    74281 tcagtacgtg atcgcgccat tgtccatcaa tcaacagata gtctttcgca tagccttctt
    74341 tttcaaaacc cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc atataattag
    74401 ccataatgcg atgaatatgt tgggtgcgct gcatataacg aatggctgcg gtcagggctt
    74461 caaacatgag tcctttgccc tgccattttt gcccaatcga ataaccgaga tagcaggcat
    74521 gaaaagagcc gcgaacaaca ttggaaaaat tggcaacgcc aataatctct ttttcgtccg
    74581 ggtcgaataa gccaaagtag aaagctgaac cttgtttatg aaattcgtta atcatcccta
    74641 gcctggcctg ccagcctgat ggataacagt ggctttcgtc gcgcactggc tcccagggct
    74701 tgaggaaatg gcgattctct gcgtaataat ccgccagacg ccaggcatca cgatcatgca
    74761 ccagacgcac gaccagtcgg tctgtggtta agcgcacttt tggcacgtta ctgcgatagc
    74821 caaacattct atacctactc cttcccgtaa cgtctttaat acgctgtaag tattactata
    74881 cctgcgcaac cagaccctgt gaaaagtcca aaatgccatt ttctgcatta ttcacatatt
    74941 tgataaaaac tctctatcaa agcgacgttt tgataaaaaa atattgtccc cgaagtgatg
    75001 ggaaaagtct tagcggcaat gcagaataga aagtgctcat cttttatttc ccgggagggg
    75061 aaatgtcccg cgtatcgcag gcgaggaacc tgggtaaata tttcctgctc atcgataata
    75121 tgctggtcgt gctggggttc tttgttgtct tcccgctgat ctctatccgc ttcgttgatc
    75181 aaatgggctg ggccgccgtc atggtcggta ttgctctcgg tctacgccag tttattcagc
    75241 aaggtctggg tattttcggc ggcgcaattg ccgaccgctt tggtgccaaa ccgatgattg
    75301 ttaccggtat gctgatgcgc gccgccggat tcgccacaat gggtatcgcc cacgaaccgt
    75361 ggctattgtg gttttcatgc ctgctctcgg gactcggcgg cacgttgttt gatccgccgc
    75421 gttcggcgtt ggtggtgaaa ctaatccgtc cacaacaacg tggtcgtttt ttctcgctgt
    75481 tgatgatgca ggacagtgcc agtgcggtca ttggcgcatt gttggggagc tggctgttgc
    75541 aatacgactt tcgcctggtc tgcgccacag gggccgtttt gtttgtgcta tgtgcggcgt
    75601 tcaatgcgtg gttgttaccg gcatggaaac tctccaccgt acgcacgccc gttcgcgaag
    75661 gcatgacccg cgtgatgcgt gacaagcgtt ttgtcaccta tgtcctgacg ctggcgggtt
    75721 actacatgct ggctgtacaa gtgatgctga tgctgccaat tatggtcaac gacgtggctg
    75781 gcgcgccctc tgccgttaaa tggatgtatg ccattgaagc gtgtctgtcg ttaacgttgc
    75841 tctaccctat cgcccgctgg agtgaaaagc attttcgtct ggaataccgg ttgatggctg
    75901 ggctgttgat aatgtcatta agcatgatgc cggtgggcat ggtcagcggc ctacaacaac
    75961 ttttcaccct gatttgtctg ttttatatcg ggtcgatcat tgccgagcct gcgcgtgaaa
    76021 ccttaagtgc ttcgctggcg gacgcaagag ctcgcggcag ctatatgggg tttagccgtc
    76081 tgggtctggc gattggcggc gctattggtt atatcggtgg tggctggctg tttgacctgg
    76141 gcaaatcgac gcaccagcca gagcttccgt ggatgatgct gggaattatt ggcatcttca
    76201 ctttccttgc gctgggttgg cagtttagtc agaaacgcgc cgcgcgtcgt ttgctggaac
    76261 gcgacgcctg atttgataca cccccctgac tcctccatac tgaaagtgaa ctgacagcca
    76321 aatggaggag tcacgtgaag ctatacattt acgatcactg cccttactgc ctcaaagccc
    76381 gcatgatttt cggcctgaaa aatatccccg tcgaattaca tgttctgctc aacgacgacg
    76441 cagaaacacc cacccggatg gtcggtcaaa aacaggttcc cattctgcaa aaagatgaca
    76501 gccgctatat gccagaaagc atggatatcg ttcactatgt cgataaactc gacggcaaac
    76561 cgttactgac cggcaaacgt tcccctgcca ttgaagagtg gctgcgcaag gtcaatggct
    76621 acgccaacaa actgctgttg ccgcgttttg ccaaatcggc atttgatgag ttttctactc
    76681 ccgccgcgcg caaatatttc gtcgacaaga aagaggccag cgcgggtaat tttgccgacc
    76741 tgctggccca ctctgacggt ctgattaaga atatcagcga tgatttgcgt gcgctggaca
    76801 aactgatcgt caaaccgaac gccgtgaatg gcgaactttc ggaagatgat attcagctat
    76861 ttccgctact gcgtaatctg acgctggtag ccggaatcaa ctggccaagc cgcgttgctg
    76921 attaccacga taatatggcg aaacagacac aaatcaattt gttatcatca atggcgattt
    76981 aatcctgacc cgccgcggca gcctgtattc gcgggttgcc gccgttttca gttttttctg
    77041 ctggcgagtt tatcgctggt gacacatgct gcgttgtgtc aggatattga cgcagatttc
    77101 atgtcgagga ctaccatgaa caagttttta tttgccgctg cattgatcgt cagtggcctg
    77161 ctcgttggct gtaatcaact cacccaatac accatcaccg aacaagaaat taaccagtcg
    77221 cttgcgaaac ataataattt ctcaaaagat atcggtttac ccggcgtggc tgacgcccat
    77281 attgttctga caaacttgac cagccaaatt ggtcgcgaag agccgaataa ggttacccta
    77341 accggagacg ccaatctgga catgaactcc ctgttcggta gtcagaaagc gaccatgaaa
    77401 ctgaagctga aagcgctgcc cgtgtttgat aaagaaaaag gtgcgatctt cctgaaagag
    77461 atggaagtgg tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg
    77521 cttccctatt tgaaccaggc attacgcaat tactttaacc agcaacctgc ttacgtcctg
    77581 cgcgaagatg gcagtcaggg cgaagcaatg gcgaaaaaac tggcgaaagg cattgaagtg
    77641 aagccaggtg aaattgtcat tccgtttacg gattaatgac gagggcgcat tcgcgccctt
    77701 tatttttcgt gcaaaggaaa acatttccgc ttatcctttg tgtccggcaa aaacatccct
    77761 tcagccggag catagagatt aatgactgca ccatcccagg tattaaagat ccgccgtcca
    77821 gacgactggc accttcacct ccgcgatggc gacatgttac aaactgtcgt gccgtatacc
    77881 agcgaaattt atggacgggc tatcgtaatg cccaatctgg ctccgcccgt gaccacagtt
    77941 gaggctgccg tggcgtatcg ccaacgtatt cttgacgccg tacctgccgg gcacgatttc
    78001 accccattga tgacctgtta tttaacagat tcgctggatc ctaatgagct ggagcgcgga
    78061 tttaatgaag gcgtgttcac cgctgcaaaa ctttacccgg caaacgcaac cactaactcc
    78121 agccacggcg tgacgtcaat tgacgcaatc atgccggtac ttgagcgcat ggaaaaaatc
    78181 ggtatgccgc tactggtgca tggtgaagtg acacatgcag atatcgacat ttttgatcgt
    78241 gaagcgcgct ttatagaaag cgtgatggaa cctctgcgcc agcgcctgac tgcgctgaaa
    78301 gtcgtttttg agcacatcac caccaaagat gctgccgact atgtccgtga cggaaatgaa
    78361 cggctggctg ccaccatcac tccgcagcat ctgatgttta accgcaacca tatgctggtt
    78421 ggtggcgtgc gtccgcacct gtattgttta cccatcctca aacgcaatat tcaccaacag
    78481 gcattgcgtg aactggtcgc cagcggtttt aatcgagtat tcctcggtac ggattctgcg
    78541 ccacatgcac gtcatcgcaa agagagcagt tgcggctgcg cgggctgctt caacgcccca
    78601 accgcgctgg gcagttacgc taccgtcttt gaagagatga atgctctgca gcactttgaa
    78661 gcattctgtt ctgtaaacgg cccgcagttc tatggcttgc cggtcaacga cacattcatc
    78721 gaactggtac gtgaagagca acaggttgct gaaagcatcg cactgactga tgacaccctg
    78781 gtgccattcc tcgccgggga aacggtacgc tggtccgtta aacaataaaa atcgtagctt
    78841 cctgttgtca ttaggttatt ttacctgtat aaataaccag tatattcaac agggggctat
    78901 tatgcgaatt gaagtcacca tagcgaaaac ttctccattg ccagctgggg ctattgacgc
    78961 cctggctggc gaactttccc gccgtattca gtatgcgttt cctgataatg aaggccacgt
    79021 atcggtacgt tatgccgcag cgaataattt atcggttatt ggcgcaacaa aagaagataa
    79081 acagcgcatt agcgaaattc tccaggaaac gtgggaaagc gccgatgact ggtttgtcag
    79141 cgaataatat gcagtgattt ttgtttttgc cgggtcgccc cggctttttt attttactta
    79201 cgaattttga gtacaaaatt tctacacttc ctcaaaataa ttgcacaata tgctaaaaaa
    79261 atatgaatga catgctgttt tattgttcat ataaatctta aaagcagaaa tttatctgtt
    79321 ccccccttta aaaattcggt gaataccctt acttattggt atactgaagt tgcttcagat
    79381 aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg gccatgatgg
    79441 aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc agcaccgttg
    79501 atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag tccgagcagg
    79561 aagggactga agttggtcag acgctctggt taaccactga tgttgccaga caatttattt
    79621 cgatattaga agcaggaatc gccaaaattg aatccggtga tttcccggta aacgagtatc
    79681 ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg cctttaccat
    79741 ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag agcagagaga
    79801 aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct gccgccgggt
    79861 attatgcaac ccgcgccggt ttaaacgtgc taatgaccga cgcccatatg ccaccgcatc
    79921 aacacggcag ccaccacggc gatacgcgat taattcgcca tgcttatggt gaaggcgaaa
    79981 agtatgtccc gttagtcctc cgcgcgcaaa cgctgtggga tgaactttcc cgccacaacg
    80041 aagatgatcc catttttgta cgctctggtg tcattaacct cggcccggct gactccgcat
    80101 ttctcgccaa cgtcgcccac agcgccgaac agtggcaact caacgttgaa aagctcgatg
    80161 cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac atcggcttat
    80221 ttgagactga ttccggtttt ttgcgcagcg aactggcgat taaaacctgg atccaactgg
    80281 cgaaggaagc gggctgtgcg caactgttca actgcccggt caccgcaatt cgtcatgacg
    80341 atgatggcgt aactattgaa acggttgacg gtgagtatca ggcgaaaaaa gcgattgtct
    80401 gcgcgggaac atgggtaaaa gacctgctcc cggagctgcc tgtccagccc gtacgtaaag
    80461 tatttgcctg gtatcaggcc gatggccgct atagcgtgaa gaataaattc ccggcgttta
    80521 ccggtgaact gcccaatggc gatcaatatt atggttttcc ggcagaaaac gacgcgttga
    80581 agattggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt gttccgtttg
    80641 cggaagtggc cagtgatggg tcagaagcct tcccgttctt gcgcaatgta ttgccgggta
    80701 tcggttgctg cctgtacggc gctgcctgca cctatgataa ttcgcctgac gaagatttta
    80761 ttatcgatac cctacccggc cacgataata cactgctcat taccggcctg agtgggcacg
    80821 gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa gaccaaaaaa
    80881 gcgattttga tttgacgcca ttcaggcttt cccgcttcca ataatcatca ttacggcctc
    80941 cagaatgagg ccgtttttac aggagtattt aatgcgtcgg ctgttgcact atctcatcaa
    81001 taatattcgc gagcatctga tgctttatct ttttctctgg ggattgttgg caattatgga
    81061 tctgatctat gtgttttatt tttagttttc cgcactgaca taaactgaca aatatttaat
    81121 ctccctttca tttagcgtga aaaattgaat gagattattc ctgcttctac agcactcacc
    81181 agacatctct gatgctgaac ccactaaact tatgaagatt ctttcattat taattcaaaa
    81241 ttattgaaat aaaaataaat atcattcaat ttgttatttt tgtgttgcaa ttcattctcc
    81301 gacacgccat tttctttctc tcgcaaattc gtattggatg cacttatgtc attcacaaat
    81361 acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc aattattgtc
    81421 tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga tggctggtat
    81481 cacaaagcac ccgaactgca taaaagcatc ggtattttgt taatgatggg gctggttatt
    81541 cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta ttcgcccatg
    81601 actcgtcttg ccgcaaaagc tggacatctc gcattgtacc tattgctgtt cgccatcggc
    81661 atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt tggctggttt
    81721 gacgtccccg cgacccttgc cgacgctggc gcacaggcag actttgccgg tgccctgcat
    81781 ttttggctcg cgtggagcgt cgtagtactg tctgttatgc acggatttat ggccctgaag
    81841 catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc gtcatctgac
    81901 tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc
    81961 tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca gcacgccttt
    82021 gttaacttcc gcatacagca tcttggctat agctggttat atggcacctt taaagatttc
    82081 gacggtactt ttacctttga tgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt
    82141 aacaccacca gcgtcgatac taaccacgcc gaacgcgata aacatcttcg cagtgcggat
    82201 tttctcaata ccacaaaata tccacaggca acattcacct ccaccagcgt gaagaaagac
    82261 ggtgacgaac tggatattac cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg
    82321 ctggaagcga aattaattgg tcagggcgac gacccatggg gcggtaaacg tgcaggcttt
    82381 gaggccgaag gcaaaattaa gctcaaagac ttcaatatca agacagattt aggtccagct
    82441 tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta agagtgaaat
    82501 gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg aatacccagt
    82561 gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc
    82621 cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat ctcactacaa
    82681 caacctttgt atttttccgc gcatactgga cactgaataa acagcaggtg gcagccatca
    82741 tttttacagt tggtatggct gtcgcacggc gcgccgcact ggtggcaatg cgcgataatc
    82801 tcatccgata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgc
    82861 accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccgcc ctcgatatgc
    82921 cagactttgt tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg
    82981 ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc gactgctttt
    83041 ggcagctgct cacggaaggt atctgccgga atttcgagcg cgttttcaaa gtgccccact
    83101 tcatactcat agtggttacg catgtcgata aatagtgcat cgggatcgtc aagcatggcg
    83161 ttcacttccg ccgcttgcag atactcacca acattgctgg catcaaagtg aggatcgtca
    83221 ataccgtcgg caacaatgcg atcgcgtacc ttcatgcgca gtacccagaa ggatttcccg
    83281 tcatcatcca acgcgatatt caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc
    83341 tgcgcgcgaa atgtttcaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca
    83401 tgcgccagat acactcgccc aaaaacattc agcgcggtaa acagctgata taaagcgtca
    83461 cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt
    83521 tcgctctcag ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact
    83581 ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat
    83641 tatcgataat taacatccac acattttacg ctacatttgc gcattaaaaa ttatttgtta
    83701 tttacaagcg cggcaatttc gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa
    83761 atgcgacaat acatacaatt gcccgtatag gttgaaaaac aggattgata tgacgaatct
    83821 acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg
    83881 cgtactttgg ttggtcgtgc aattgcccta cccgattatc taccgcctcg gttgtggatt
    83941 aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga
    84001 actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga
    84061 atccgttggc atgggcctga tggaaaccgg tatggcgtgg ttctggccgg accgccgaat
    84121 cgcccgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg
    84181 cggcatcctg ttagttggca tccattttct gacactggag ctgggcgcgc ggcagtttgg
    84241 tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct
    84301 acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg
    84361 catgattaaa gccctgaaaa aaggcgaagt ggtctggtac gcaccagatc atgattacgg
    84421 cccgcgctca agcgttttcg tcccgttatt tgccgttgag caggctgcga ccacgaccgg
    84481 aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa
    84541 gccagatggc aaagggtatc aattgattat gctgccgcca gagtgttctc cgccactgga
    84601 tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc
    84661 accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc
    84721 acgctattaa atctcccatg ccggatgctt cagaatggca tccggcatta ccacagcaaa
    84781 tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa
    84841 ttagtgtgtt tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc
    84901 tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg
    84961 gctaggctgt tttcttaccg gggccgcctt cagtctggta atgcccttct tacccctcta
    85021 cgttgagcag cttggtgtta ccggccactc cgccctgaat atgtggtccg gtattgtctt
    85081 cagtataatg ggttcctaaa cacatcattt tagatattga atatgagacg ggcaatcagc
    85141 tacatcagat tttcaagtga acgacaactg aaaggggact ctgtacgccg acaatctaag
    85201 ctggttactg actggcttga taagaaccct gagttttact tggattcatc cttgagcttt
    85261 aaagatttgg ggaaaagtgc ttttagtggt aagcacctga aaggtggtct aggtgacttt
    85321 ttaaccgcta tcgagaaagg gcttgttaaa gctggtgata cgctactaat cgagagttta
    85381 gaccgtctgt cacgtcagga tattgatatt gcttctgagc tattacgccg tatcttaagg
    85441 gctggtgtag atgtagtcac actgagcgat ggagaacatt acacacgaga aagtttaaaa
    85501 gacccgttag ccctgattaa aagcattctg ataatgcagc gggcgcatga ggaaagtttg
    85561 aggaagtctg agcgtgtcca agctgcatgg aaccgtaaaa aagaactcat ctccgaaggt
    85621 atcaaggtat ctaggcgttg tcctgcatgg ttaaggctga atgatgaccg tcgaacattc
    85681 acaatcattc ctgataaagt ggaagttgtt aaacgagcat ttgacctgag attacagggt
    85741 ttaagctttt gggctatcac cagaacttta aacgatgaag gtcacttgtc attgaatcaa
    85801 tacacaccca aacaaaaggg gtggtctgat actgctgtaa agaaactact cagaaatcgt
    85861 gctgtgattg gatgtttcac acctgctgga cgtgaagaag ttcagggtta ctaccccgca
    85921 ataatcagtg agtcactatt ctatcgagta cagcaactga acacagggca atatggtagg
    85981 gcttctgtat caagtaatcc gctatcagtc aacttgttca ggggtatcat caaatgttct
    86041 gagtgtgggg ctactattgt tcttggtgga tatgccttaa aacgttttgg aatgtacaga
    86101 tgccctaatc gttcagctaa caggtgtagt gcaaaggcta tcagtcgtaa gcaaacggat
    86161 gcaactatcc tttacatgct cgcattatgc gaccgttttg aaactgaaaa tactgacact
    86221 attgatagcc ttaaattaca gcgtgaagat ttg
//
LOCUS       DAJTYF010000004        82506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTYF010000004 DAJTYF010000000
VERSION     DAJTYF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.004"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..368)
                     /locus_tag="OO590_000338"
     CDS             complement(<1..368)
                     /locus_tag="OO590_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR6635835.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLG"
     gene            complement(439..2553)
                     /gene="fusA"
                     /locus_tag="OO590_000339"
     CDS             complement(439..2553)
                     /gene="fusA"
                     /locus_tag="OO590_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6635836.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2650..3120)
                     /gene="rpsG"
                     /locus_tag="OO590_000340"
     CDS             complement(2650..3120)
                     /gene="rpsG"
                     /locus_tag="OO590_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6635837.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3217..3591)
                     /gene="rpsL"
                     /locus_tag="OO590_000341"
     CDS             complement(3217..3591)
                     /gene="rpsL"
                     /locus_tag="OO590_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6635838.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3717..4004)
                     /gene="tusB"
                     /locus_tag="OO590_000342"
     CDS             complement(3717..4004)
                     /gene="tusB"
                     /locus_tag="OO590_000342"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6635839.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4012..4371)
                     /gene="tusC"
                     /locus_tag="OO590_000343"
     CDS             complement(4012..4371)
                     /gene="tusC"
                     /locus_tag="OO590_000343"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6635840.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4371..4757)
                     /gene="tusD"
                     /locus_tag="OO590_000344"
     CDS             complement(4371..4757)
                     /gene="tusD"
                     /locus_tag="OO590_000344"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6635841.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4757..5479)
                     /locus_tag="OO590_000345"
     CDS             complement(4757..5479)
                     /locus_tag="OO590_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6635842.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5646..6458)
                     /gene="fkpA"
                     /locus_tag="OO590_000346"
     CDS             complement(5646..6458)
                     /gene="fkpA"
                     /locus_tag="OO590_000346"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6635843.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6679..6897
                     /locus_tag="OO590_000347"
     CDS             6679..6897
                     /locus_tag="OO590_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6635844.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6946..7536)
                     /gene="slyD"
                     /locus_tag="OO590_000348"
     CDS             complement(6946..7536)
                     /gene="slyD"
                     /locus_tag="OO590_000348"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6635845.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETEQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7631..7831)
                     /locus_tag="OO590_000349"
     CDS             complement(7631..7831)
                     /locus_tag="OO590_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6635846.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7841..9646)
                     /gene="kefB"
                     /locus_tag="OO590_000350"
     CDS             complement(7841..9646)
                     /gene="kefB"
                     /locus_tag="OO590_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR6635847.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9646..10197)
                     /gene="kefG"
                     /locus_tag="OO590_000351"
     CDS             complement(9646..10197)
                     /gene="kefG"
                     /locus_tag="OO590_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6635848.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10328..12241
                     /locus_tag="OO590_000352"
     CDS             10328..12241
                     /locus_tag="OO590_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6635849.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPEQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12241..13263
                     /locus_tag="OO590_000353"
     CDS             12241..13263
                     /locus_tag="OO590_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6635850.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13257..13475
                     /locus_tag="OO590_000354"
     CDS             13257..13475
                     /locus_tag="OO590_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6635851.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13529..14398
                     /locus_tag="OO590_000355"
     CDS             13529..14398
                     /locus_tag="OO590_000355"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6635852.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14453..14857)
                     /locus_tag="OO590_000356"
     CDS             complement(14453..14857)
                     /locus_tag="OO590_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6635853.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15159..15791
                     /gene="crp"
                     /locus_tag="OO590_000357"
     CDS             15159..15791
                     /gene="crp"
                     /locus_tag="OO590_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6635854.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15842..17932
                     /locus_tag="OO590_000358"
     CDS             15842..17932
                     /locus_tag="OO590_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6635855.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17999..19219)
                     /gene="argD/dapC"
                     /locus_tag="OO590_000359"
     CDS             complement(17999..19219)
                     /gene="argD/dapC"
                     /locus_tag="OO590_000359"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6635856.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19305..19868)
                     /gene="pabA"
                     /locus_tag="OO590_000360"
     CDS             complement(19305..19868)
                     /gene="pabA"
                     /locus_tag="OO590_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6635857.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19900..20502)
                     /locus_tag="OO590_000361"
     CDS             complement(19900..20502)
                     /locus_tag="OO590_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6635858.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20492..20659)
                     /locus_tag="OO590_000362"
     CDS             complement(20492..20659)
                     /locus_tag="OO590_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6635859.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20764..21336)
                     /gene="ppiA"
                     /locus_tag="OO590_000363"
                     /gene_synonym="rotA"
     CDS             complement(20764..21336)
                     /gene="ppiA"
                     /locus_tag="OO590_000363"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6635860.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21607..22788
                     /gene="tsgA"
                     /locus_tag="OO590_000364"
     CDS             21607..22788
                     /gene="tsgA"
                     /locus_tag="OO590_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6635861.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23050..25593
                     /gene="nirB"
                     /locus_tag="OO590_000365"
     CDS             23050..25593
                     /gene="nirB"
                     /locus_tag="OO590_000365"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6635862.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKGDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25590..25916
                     /gene="nirD"
                     /locus_tag="OO590_000366"
     CDS             25590..25916
                     /gene="nirD"
                     /locus_tag="OO590_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6635863.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26042..26848
                     /gene="nirC"
                     /locus_tag="OO590_000367"
     CDS             26042..26848
                     /gene="nirC"
                     /locus_tag="OO590_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6635864.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26867..28240
                     /gene="cobA"
                     /locus_tag="OO590_000368"
     CDS             26867..28240
                     /gene="cobA"
                     /locus_tag="OO590_000368"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6635865.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28487..28654
                     /locus_tag="OO590_000369"
     CDS             28487..28654
                     /locus_tag="OO590_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6635866.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28949..30286
                     /gene="frlA"
                     /locus_tag="OO590_000370"
                     /pseudo
     CDS             28949..30286
                     /gene="frlA"
                     /locus_tag="OO590_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30307..31329
                     /gene="frlB"
                     /locus_tag="OO590_000371"
     CDS             30307..31329
                     /gene="frlB"
                     /locus_tag="OO590_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6635867.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITLLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31379..32209
                     /gene="frlC"
                     /locus_tag="OO590_000372"
     CDS             31379..32209
                     /gene="frlC"
                     /locus_tag="OO590_000372"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6635868.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIYMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32206..32991
                     /gene="frlD"
                     /locus_tag="OO590_000373"
     CDS             32206..32991
                     /gene="frlD"
                     /locus_tag="OO590_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6635869.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33091..33822
                     /gene="phoB"
                     /locus_tag="OO590_000374"
     CDS             33091..33822
                     /gene="phoB"
                     /locus_tag="OO590_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6635870.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33982..35067)
                     /locus_tag="OO590_000375"
     CDS             complement(33982..35067)
                     /locus_tag="OO590_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6635871.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35079..36383)
                     /locus_tag="OO590_000376"
     CDS             complement(35079..36383)
                     /locus_tag="OO590_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6635872.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEMALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36395..36686)
                     /locus_tag="OO590_000377"
                     /pseudo
     CDS             complement(36395..36686)
                     /locus_tag="OO590_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 family protein"
     gene            complement(36697..37575)
                     /locus_tag="OO590_000378"
     CDS             complement(36697..37575)
                     /locus_tag="OO590_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6635873.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37572..38798)
                     /locus_tag="OO590_000379"
     CDS             complement(37572..38798)
                     /locus_tag="OO590_000379"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6635874.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38799..39962)
                     /locus_tag="OO590_000380"
     CDS             complement(38799..39962)
                     /locus_tag="OO590_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6635875.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(40046..40408)
                     /locus_tag="OO590_000381"
     CDS             complement(40046..40408)
                     /locus_tag="OO590_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6635876.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40425..41330)
                     /locus_tag="OO590_000382"
     CDS             complement(40425..41330)
                     /locus_tag="OO590_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635877.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41600..42604)
                     /gene="trpS"
                     /locus_tag="OO590_000383"
     CDS             complement(41600..42604)
                     /gene="trpS"
                     /locus_tag="OO590_000383"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6635878.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42597..43355)
                     /gene="gph"
                     /locus_tag="OO590_000384"
     CDS             complement(42597..43355)
                     /gene="gph"
                     /locus_tag="OO590_000384"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6635879.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43348..44025)
                     /gene="rpe"
                     /locus_tag="OO590_000385"
     CDS             complement(43348..44025)
                     /gene="rpe"
                     /locus_tag="OO590_000385"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6635880.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44043..44879)
                     /gene="dam"
                     /locus_tag="OO590_000386"
     CDS             complement(44043..44879)
                     /gene="dam"
                     /locus_tag="OO590_000386"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6635881.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44986..46272)
                     /gene="damX"
                     /locus_tag="OO590_000387"
     CDS             complement(44986..46272)
                     /gene="damX"
                     /locus_tag="OO590_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6635882.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46364..47452)
                     /gene="aroB"
                     /locus_tag="OO590_000388"
     CDS             complement(46364..47452)
                     /gene="aroB"
                     /locus_tag="OO590_000388"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6635883.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47509..48030)
                     /gene="aroK"
                     /locus_tag="OO590_000389"
     CDS             complement(47509..48030)
                     /gene="aroK"
                     /locus_tag="OO590_000389"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6635884.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48431..49669)
                     /gene="hofQ"
                     /locus_tag="OO590_000390"
     CDS             complement(48431..49669)
                     /gene="hofQ"
                     /locus_tag="OO590_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6635885.1"
                     /translation="MKQWIAALLLMLISGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49581..49985)
                     /gene="hofP"
                     /locus_tag="OO590_000391"
     CDS             complement(49581..49985)
                     /gene="hofP"
                     /locus_tag="OO590_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR6635886.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADIRRTGGKAAKSDADGG"
     gene            complement(49975..50415)
                     /gene="hofO"
                     /locus_tag="OO590_000392"
     CDS             complement(49975..50415)
                     /gene="hofO"
                     /locus_tag="OO590_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6635887.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50399..50938)
                     /gene="hofN"
                     /locus_tag="OO590_000393"
     CDS             complement(50399..50938)
                     /gene="hofN"
                     /locus_tag="OO590_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6635888.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50938..51717)
                     /gene="hofM"
                     /locus_tag="OO590_000394"
     CDS             complement(50938..51717)
                     /gene="hofM"
                     /locus_tag="OO590_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6635889.1"
                     /translation="MTFKIWQIGLHLQQQEVAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51837..54389
                     /gene="mrcA"
                     /locus_tag="OO590_000395"
     CDS             51837..54389
                     /gene="mrcA"
                     /locus_tag="OO590_000395"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6635890.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54554..55114)
                     /gene="nudE"
                     /locus_tag="OO590_000396"
     CDS             complement(54554..55114)
                     /gene="nudE"
                     /locus_tag="OO590_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6635891.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55434..57569
                     /gene="igaA"
                     /locus_tag="OO590_000397"
     CDS             55434..57569
                     /gene="igaA"
                     /locus_tag="OO590_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6635892.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57634..58302
                     /gene="yrfG"
                     /locus_tag="OO590_000398"
     CDS             57634..58302
                     /gene="yrfG"
                     /locus_tag="OO590_000398"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6635893.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58313..58714
                     /gene="hslR"
                     /locus_tag="OO590_000399"
     CDS             58313..58714
                     /gene="hslR"
                     /locus_tag="OO590_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6635894.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58739..59617
                     /gene="hslO"
                     /locus_tag="OO590_000400"
     CDS             58739..59617
                     /gene="hslO"
                     /locus_tag="OO590_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6635895.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59752..61476)
                     /locus_tag="OO590_000401"
     CDS             complement(59752..61476)
                     /locus_tag="OO590_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6635896.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61855..63477
                     /gene="pckA"
                     /locus_tag="OO590_000402"
     CDS             61855..63477
                     /gene="pckA"
                     /locus_tag="OO590_000402"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6635897.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63553..64905)
                     /gene="envZ"
                     /locus_tag="OO590_000403"
     CDS             complement(63553..64905)
                     /gene="envZ"
                     /locus_tag="OO590_000403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6635898.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64902..65621)
                     /gene="ompR"
                     /locus_tag="OO590_000404"
     CDS             complement(64902..65621)
                     /gene="ompR"
                     /locus_tag="OO590_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6635899.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65849..66325
                     /gene="greB"
                     /locus_tag="OO590_000405"
     CDS             65849..66325
                     /gene="greB"
                     /locus_tag="OO590_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6635900.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66422..68743
                     /locus_tag="OO590_000406"
     CDS             66422..68743
                     /locus_tag="OO590_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6635901.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69181..69408
                     /gene="feoA"
                     /locus_tag="OO590_000407"
     CDS             69181..69408
                     /gene="feoA"
                     /locus_tag="OO590_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6635902.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69425..71746
                     /gene="feoB"
                     /locus_tag="OO590_000408"
     CDS             69425..71746
                     /gene="feoB"
                     /locus_tag="OO590_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6635903.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGMGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71746..71982
                     /gene="feoC"
                     /locus_tag="OO590_000409"
     CDS             71746..71982
                     /gene="feoC"
                     /locus_tag="OO590_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6635904.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72185..73063
                     /gene="rpnA"
                     /locus_tag="OO590_000410"
     CDS             72185..73063
                     /gene="rpnA"
                     /locus_tag="OO590_000410"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6635905.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            73136..73405
                     /locus_tag="OO590_000411"
     CDS             73136..73405
                     /locus_tag="OO590_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6635906.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73399..73764
                     /locus_tag="OO590_000412"
     CDS             73399..73764
                     /locus_tag="OO590_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6635907.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73765..74553)
                     /gene="bioH"
                     /locus_tag="OO590_000413"
     CDS             complement(73765..74553)
                     /gene="bioH"
                     /locus_tag="OO590_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6635908.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74591..75274
                     /gene="gntX"
                     /locus_tag="OO590_000414"
     CDS             74591..75274
                     /gene="gntX"
                     /locus_tag="OO590_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6635909.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75333..75908
                     /gene="nfuA"
                     /locus_tag="OO590_000415"
     CDS             75333..75908
                     /gene="nfuA"
                     /locus_tag="OO590_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6635910.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76269..77573
                     /gene="gntT"
                     /locus_tag="OO590_000416"
     CDS             76269..77573
                     /gene="gntT"
                     /locus_tag="OO590_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6635911.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77618..79702)
                     /gene="malQ"
                     /locus_tag="OO590_000417"
     CDS             complement(77618..79702)
                     /gene="malQ"
                     /locus_tag="OO590_000417"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6635912.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTTTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79712..82105)
                     /gene="malP"
                     /locus_tag="OO590_000418"
     CDS             complement(79712..82105)
                     /gene="malP"
                     /locus_tag="OO590_000418"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6635913.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19786 a        22408 c        20820 g        19492 t
ORIGIN      
        1 cccagcagga tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg
       61 atcgcgccgt ccatctgagc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc
      121 gggcagtcta cgtgtgcgta gtgacgggtc ggggtgtcat attcaacgtg agaagtgttg
      181 atggtgatac cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcacgagca
      241 gcaccgccgt aggttttagc cagtacggtg gtgattgcag cggtcagagt agttttaccg
      301 tggtcaacgt ggccgatagt accaacgtta acgtgcggtt ttgtacgttc aaatttttct
      361 ttagacacgg ctatattcct tactatagtg ctctcccctt caggagagag cacgggactt
      421 tggtattaac ccttaggctt atttaccacg ggcttcaatt acggcctgag caacgttact
      481 cggcgcttca tcatacttca ggaattccat agtgtatgat gcacgacctt tggtcagaga
      541 acgcagctga gttgcgtatc cgaacatttc agacagcggt acttcagcgt ggatcttaac
      601 gccagtaact tcagattcct gacctttgag cataccacga cgacggctca agtcaccgat
      661 aacgtcaccg gtgttctctt ccggagtttc tacttcaacc ttcatgatcg gctcaagcag
      721 aactggtttc gctttcttaa agccttcttt aaaggcgata gaagcagcca gtttaaacgc
      781 cagttcagag gagtcaacgt catggtaaga accgaagtgc agacgaatac ccatgtctac
      841 taccgggtag cctgccagcg gacctgcttt cagctgttcc tggatacctt tatcaacggc
      901 cgggatgtat tcgccaggga ttacaccacc tttaatgtcg ttgatgaact cgtagccttt
      961 cgggtttgaa cccggctcca gcgggtacat gtcgataaca acatgaccat actgaccacg
     1021 accaccagac tgtttcgcgt gtttaccttc aacatcggta actttctggc ggatagtttc
     1081 acggtaagca acctgcggtt tacctacgtt cgcttcaacg ttgaattcac gcttcatacg
     1141 gtcaacgatg atgtcgaggt gcagttcgcc catacccgcg atgatggtct ggttagattc
     1201 ttcgtcagtc catacacgga aagacgggtc ttctttagcc agacggccca gagccagacc
     1261 cattttttcc tggtcagctt tggttttcgg ttcaactgcg atggagatta ccggctcagg
     1321 gaattccata cgttccagaa tgatcggcgc atccgggtca cacagggtgt caccagtggt
     1381 tacgtctttc agaccgatag cagcagcgat gtcgcccgcg cgaacttctt tgatctcttc
     1441 acgtttgtta gcgtgcatct gaacgatacg accgaaacgc tcacgtgcag ctttcacgga
     1501 gttcagtacg gtatcaccag agttaaccac accggagtaa acacggaaga aggtcaggtt
     1561 accaacaaac gggtcggtag cgattttgaa cgccagtgca gagaacggct cgtcatcact
     1621 tgcgtgacgt tcagccggag tgtctttacc gtcgtccagg ataccgttga tcgcaggtac
     1681 gtcaaccggg gatggcaggt aatcaattac cgcatccagc atcgcctgaa cacctttgtt
     1741 cttgaacgca gaaccacagg ttaccaggat gatttcgttg ttcagaacgc gctgacgcag
     1801 agcacctttg atttctgctt cagtcagttc ttcaccaccc aggtattttt ccatcagctc
     1861 ttcagaagct tcagctgcgg attcgatcag gttctggtgc cattcgttag ccagttcaac
     1921 catgtctgcc gggatatctt cgtattcgaa ggttacgccc tggtcagcgt cgttccagtt
     1981 gatagctttc attttcacca ggtcaacaac accggtgaaa tgttcttcag caccaatcgc
     2041 cagctgcagc ggaaccgggt tcgcgcccag acgggttttg atctggttaa caactttcag
     2101 gaagttcgca cccatgcggt ccattttgtt aacgaacgca atgcgcggaa ctttatattt
     2161 gtttgcctga cgccatacgg tttcagactg cggctgaaca ccaccaactg cgcagtaaac
     2221 cattaccgca ccatcgagaa cacgcatgga acgttctact tcgattgtga agtcaacgtg
     2281 ccccggggtg tcgatgatgt tgatgcgatg cggctcatac tgcttagcca taccagacca
     2341 gaatgcagta gtcgcagcgg aagtgatggt aataccacgt tcctgctcct gctccatcca
     2401 gtccatggtt gcagcgccgt catgaacttc accgatttta tggtttacac cggtgtagaa
     2461 cagaatacgt tcggtagtag tggttttacc ggcgtcgatg tgcgcactga taccgatgtt
     2521 acggtagcgt gcgatgggtg ttgtacgagc catttgtttc ctcgtttatc ttttaggcgt
     2581 tcaatttaag tagcccaaag cgggctgctt actggaagcg cccgcctggt gactaaaact
     2641 ccgaagggat taccaacggt agtgtgcgaa cgccttgttg gcttcggcca tacggtgaac
     2701 gtcttcacgt ttcttaactg cagtaccttt gttttctgca gcatcagaaa gttcgttcgc
     2761 caggcgcaga gccatggatt tatcaccgcg tttacgagca gcttcaacga tccaacgcat
     2821 tgccagagca ttacgacgaa ccggacggac ttcaactggt acctgataag tagaaccacc
     2881 aacgcggcga gacttaactt ctacagtcgg gcgcacgttt tcgagagcta cttcgaatgc
     2941 ttccagttca gatttaccag agcgctgagc cagggtctcc agcgcgctgt atacgataga
     3001 ttcagcagta gattttttac catctaccat caggatattt acaaatttag ccagcagttc
     3061 tgatccgaac ttcggatccg gcagaatttt acgctgacca atgacgcgac gacgtggcat
     3121 ggaaatactc cgttgttaat tcaggattgt ccaaaactct acgagtttag tttgacattt
     3181 aagttaaaac gtttggcctt acttaacgga gaaccattaa gccttaggac gcttcacgcc
     3241 atacttggaa cgagcctgct tacggtcttt aacgccggag cagtcaagcg caccacgtac
     3301 ggtgtggtaa cgaacacccg ggaggtcttt aacacgaccg ccacggatca ggatcacgga
     3361 gtgctcctgc aggttgtgac cttcaccacc gatgtaggaa gtcacttcga aaccgttagt
     3421 cagacgaaca cggcatactt tacgcagcgc ggagttcggt tttttaggag tggtagtata
     3481 tacacgagta catacgccac gtttttgcgg gcatgcttcc agcgcaggca cgttgctttt
     3541 cgcaactttg cgagcacgtg gtttgcgtac cagctggtta actgttgcca ttaaatagct
     3601 cctggtttta gcttttgctt cgtaaacacg taataaaacg tcctcacaca atatgaggac
     3661 gccgaatttt agggcgatgc cgaaaaggtg tcaagaaata tacaacgatc ccgccatcac
     3721 caggccatct ggctggagtg cttaaccgta agtctgacga aatcagtata gtcaatggga
     3781 atgatgtcgt tcaaaatttg aacagtcaaa ccgcgggcaa taaggtcttc gttcagggca
     3841 tagaccttaa tgggggcatt acgcagactt tcaaggtagc ggttaccgtc aactgcggca
     3901 gttacgccat cttgcaataa tagcagttcg tctccttcac tgagcagacg cagcagcgca
     3961 gcaaaatccg tcagccaggg tgaacgatgt aacgtgtgca gcataaacgc ctcaaaacct
     4021 caaaataaca tcgtagttgg cgagttcgcg gcgtaaggca tctgcttcga gcggcgtggc
     4081 ttcgacaaca aagggtgtct gcggatctaa cccgcgttcg cgcagtgaag ccgcacaaac
     4141 ccagcactgt tcaatgtcgt acagactcaa caatttaaaa gtggcaatgt aatcacgcgc
     4201 cagcactgca tcgggctttt gtcctgacag cagctgaaaa acgccatcag caataaagaa
     4261 gacggccaga tcgtcagtta atgcggaagt tgccagtaaa gcatctaaac cttcccgacc
     4321 agcagctgta ccatgaggtg cagtagaaaa aacaaacgca attcgtttca tcagaactgt
     4381 accaccctgt cacaggtcag cgaggcttcc gccagcgcgc caagtccgct taaggtaaat
     4441 cccggctgaa ggtttgacga agccagcccc agccttcctg cttcagtttc atcgacgata
     4501 ccgcggcgta acgctgccgc tacgcagata ttcagcgcca caccatgttg cgcattcaac
     4561 tgttgccagc cgcgcacaag gtcaaattcg tcgcttgccg gtgaggtcag ttggttagcg
     4621 ttatagaccc cttcccgata gaaaaagacg ctgcttaact tatggccttc tgctatcagc
     4681 gcctgcgcaa actgaaaagc actactcgcc tgttgcgtac cgtatgctgg cccggtcacc
     4741 acgatggcaa aacgcattac ttatcttgcc cctgaaaatc accgctcttg aactggcgga
     4801 tgtagagata gacagtgtgt ttggagatgt tcaggcggtc agcaacctgg ttgatcgcat
     4861 ctttaatatc gaagatccct ttctcgtaga gattcagcac gatctgacga tttttggcgt
     4921 tattagaaac attgcgatcg gcattcactt cttcgatggt gaactccagc gtttgggtaa
     4981 ccagatcttc aacagaagag gcaaagttga cgcttgaacc gacatccggg gtttctggcg
     5041 gcacaaaagt gctcataatc tgcgagaagg gaacatcaag attcatattg atgcacagca
     5101 gaccaattac acgctgttcg cggttacgaa tcgcgatagt cagggacttc attaatacgc
     5161 cgcttttggc gcgagtaaag tagcatttag aaacgctgct atccgctccc gtcatatcgt
     5221 gcagcatacg tagcgccagg tcagtaattg gcgaaccaat cttccggcct gtatgttcac
     5281 cgttagcaat gcgaatggct gaacatttta gatcctgcaa agagtgcaaa acgatttcac
     5341 agtgggagcc aataagcatc gctaacccgt ccaccaccgc ttcgtaggat ttcagaatat
     5401 caaaatcggt ctggtcgaaa ggacgttgat ccagtaaatc caactcactg gtttcgttgg
     5461 ttaaaagcga cctggacatg aaaaaaaaca ctccttttca ggagcctgtc gttaactttt
     5521 cagggcaggc tcattaatga tgcgggtaac taaattaata cagcggaggt tccgctttcc
     5581 agcactaatt atatccggcc tgtaataaaa aaaccgccgc ctggtcaggc ggcggttctt
     5641 aatgcttatt ttttagcaga atctgcggct ttcgcatcag cttccggctt tgcatcagcc
     5701 ttcggcgctg gtttcacatc cagcagctct acgtcaaaca ctagggtaga attcggtggg
     5761 atccccggaa cacccgcttt gccgtaagcc agttctggtg gaataaccag tttgatctta
     5821 ccgcctttct tgatgttctt cagaccttct gtccaacccg ggataacacc gtccagacgg
     5881 aaagaaagcg gttcaccacg ggtgtaagag ttgtcgaact ctttaccgtc gatcagcgta
     5941 cctttgtagt tcactacaac agtatcgctg tctttcggtg cttcgccttt accggcttct
     6001 actacctgat aaaccagacc agttgaagag gttttcacac ctttctcttt ggcaaatttc
     6061 tcgcggtact ctttaccttt tgcttcgtta tcagccgcgt ctttttccat cttcgcctga
     6121 gcagaagact tcacgcgagc ttcgaatgct tgcagagtct gttcgatctc ttggtcggag
     6181 agtttgctct tatcagcaaa tgcatcctga acaccagcga tcagctgatc tttatccagt
     6241 ttgatgccca gtttttcttg ttctttaaga gagttttcca tgtaacgacc cagcgaagca
     6301 cccagtgcat aagctgattt ctgatcgtca tttttgaacg ctgctttgct gtcagcagtt
     6361 gtagcaggtt ttgcagcttc agcagcaaaa gtgattggtg catgcagggc aacggccatt
     6421 gtggtcgcca gtagcgttac tttaaacagt gatttcatcc atatctccag gatcggggca
     6481 tctcacccca gggttaacta ttatcagaag ggtactataa agcgttgtcg aacaaatcta
     6541 catacagaca cgccctatta tcatctattt tcagactctt tttgtttaaa ttagtttcga
     6601 tgaccgcgaa atgagtgctg tctcgggcag caaagttaag tagaatccgc ggcgaccatt
     6661 cgacaaaaga ggtgaatcat gcaggattta tcattggaag cacgcctggc agagctggag
     6721 agccgactgg cttttcagga aatcaccatt gaagaactga acgtcacggt gaccgctcat
     6781 gaaatggaga tggcgaaact gcgcgatcat ctgcgtctgc tgaccgagaa gttaaaagcc
     6841 agccagccgt cgaacatcgc gtcgcaggct gaagaaacgc caccgccaca ttattgaggc
     6901 gtaaaaaaag cggggattcc ccgctttttt gtcacctttt cggtattagt ggcaaccaca
     6961 accgccgttg cctttaccgc cacagcagcc ttcgccaccg tgttcatgac cgtgatcgtg
     7021 gccatgaccg ccgcagcaac cgtcgtggtc gtgatcgtgg tgatgatcgt gcgcgccgtg
     7081 aacgtggcca tgagccagtt cttcttcggt cgcttcgcga atcgccacaa cttcaacgtt
     7141 gaatttcagg ttctgaccgg ccagcatgtg gttaccatca accactacgt gatcgtcttc
     7201 aaccgcagtg atttcaaccg gtaccggacc ctgctcagtt tcagccagga aacgcatacc
     7261 tacctgcagt tcatcaacgc ccataaatac gtctttagga acacgttgca ctaggttttc
     7321 gtcgtactga ccgtaagcgt cgttcgcgcc aacagcgaca tcaaatttgt cgccaacttc
     7381 atgaccttcc agcgccgttt ccaggccaga gatcagggaa ccgtgaccat gcaggtagtc
     7441 cagcggcgca ctcaccggag actcatcaac caacacaccg tcttctgtac gtacctgata
     7501 ggccaggctg accaccaggt cttttgctac tttcatgata tctcctgagc atgggaagaa
     7561 tagtggcgca gattgtagcg gaattctgca gccgtgtact cactagctta aaaaaactcg
     7621 gcgcatatcg ctagtccgga tgaaaaatcc cgatcacttg ctcatctttg tgaacgtgat
     7681 cgcgggcttc tttgtctgct tctcgcatct gatgtccgca cttaacacat tcaacaatat
     7741 caatattatt ttcgcgccac atcgccattg aatcctgcgc ctgacaggcc gggcattttg
     7801 cgcccgctat aaaacgtttt cggattgcca tccttaccct ctactcaaat tcatcccagc
     7861 cgtccagctg gcgtcgttct tgttgcattt cacgctggaa aatctcttcc agttcgcgtc
     7921 gggcttccct ggcgcgagaa atttgtacgg tatcagcatg catcgggatt aactcccgca
     7981 gcattcgcat atccaggcgg cgaaaatgca gttgcgcccg ctgtgcctga tgcggatgca
     8041 tgccaagcgt gaccagcgtc ttgcgcccca gctctaacgc actggagaat gtttcacggg
     8101 aaaactgcgt cacccctgcc tgtaataact catgcgcttc cacacgtccg cgcactcgcg
     8161 caagaatatg caaatgcgga aagtgctgct ggcatatttc caccagcttc atggtgtctt
     8221 ccggctcgtt acaggtaatg acgatagact cagcggcctc tgcacccgca gaacgtaaaa
     8281 gatcgacctg cgtggcgtcg ccgtaataaa ctttgtagcc atatttacgc atcaggttaa
     8341 cggcgctgat atcccgctcc agcacggtaa tgcgcatttt atttgccatc agcaaacgac
     8401 caatcacctg accaaaacgc ccgaagccca cgacaatgac ctggggttta tcatcgttga
     8461 cccacggttt ttcgtcttct tcttccggtc cgttaaactg gcgggatagc catttatcca
     8521 ccagcttcat cagcaacggc gtggtcatca tggaaagcgt caccgtcacc agcaacagcg
     8581 ccatctggtc gccctggaat aaacgttgtg aagaagcggt agaaaagagg acaaaggcaa
     8641 actcaccgcc ctgactcaac acgccagcaa actgcatccg ctctgagcta cgcacgccat
     8701 acaatcgcgc cagcagatac agcacgagaa ttttcaccgc caccagcaca accacgctta
     8761 tcactaccca caacagatgg gtataaagca ccccgaggtt gagcgacatg ccgacagaga
     8821 taaagaacaa accgagcagc aagcctttga agggatcgat agccgtttcc agttcatggc
     8881 gatattcact ttccgccagc agcacgcccg caataaatgt accgagcgcc atcgacagcc
     8941 ccagcgcatc cataaacaat gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca
     9001 cttcccgcac gccagaagct gcaataaagc ggaataccgg acgcagtaaa tagcgcccac
     9061 caatcagcat gccgacaaac gccagcacct tcatgccgat cttcatccag tcgaaatgtt
     9121 cgtctgccga ccccgccaac aacggcacta acgccagtgc tgggatcact gccagatcct
     9181 gaaacagcag aaccgaaaac ccgagctggc cggattcgct gcgattcatc cctttctcac
     9241 gcatcaattg caacgccatt gcagttgaag acatcgcaag gccaatgcca ccgaccaccg
     9301 ccgcctgcca ggcgaaatcc gtcagcatca ataatcccgc cagcaacgcc gcgcttaaca
     9361 gcacctgtgc cacgcctacg ccaaaaatcg aacgccgcag ttgccaaagt ttggagggat
     9421 tcaactcaag gccgatgata aacatcagga ataccacgcc gagttccgaa aagtggagga
     9481 tctcatcgac gtcgctaata aaccccagcc cccacgggcc aattgcaatc cctgccagca
     9541 aatatcccaa cacagcgcca atccccagcc gcgatgccaa cggcaccgca gccaccgccg
     9601 cgaagagaaa cagcactcct gcgagtaaaa aatcggaacc ttccatcagc ggcctcctgg
     9661 agacagcgga tttgccaacc agtcaccgta ggctctggcg tggctcgcca gctcctttgc
     9721 gctttgccgt ctcgcccagt aaataatgat ggaacttaac caatgcatcc ggcacatgcc
     9781 cgccgccagt tcaaaggggc gcagcacatc gctcatcggg tagcgattca gcgcgtcata
     9841 acggtaagca ctttccggct cgccggtggt aatcacgtta cgccagtact ttcccgccag
     9901 ttggtttcct cccggcccgc tggcaaaacc acgacttaat acccggtcca gccactcttt
     9961 cagtagcgcc gggcagctat aggtataaag aggatgctga aagacaatca cctcgtgctc
    10021 gcgcagtaat gcctgctcac gggggatatc aataaaaaaa tcgggatagt gcgcgtaaag
    10081 gtcgtgcacg gtaacattgc tgagctgcgt ggccggttta agcagtaccc ggtttgccac
    10141 cgagtcctga gattccggat gggcatacag cagcaaaact ttcgctggct gagacatcat
    10201 ccccctcccg gtatggtttt tgtgtatagt cgctgttttg ggctaccatt gcgcccggtg
    10261 cggcagctcg cccatacatt acattatcat aatgataagt taacatagtc tgaacatacg
    10321 gcgccttatg attgttttct cctcgttaca aattcgtcgc ggcgtgcgcg tcctgctgga
    10381 taatgccacc gccaccatca accccgagca gaaagtcggc ctggtgggta aaaacggctg
    10441 tggtaaatct accctgctgg cattgctgaa aaatgaaatc agcgccgacg gcggcagcta
    10501 cacctttccg ggaagctggc aactggcgtg ggtgaatcag gaaacgccag cgttaccgca
    10561 agcggcgctg gaatatgtca ttgacggcga ccgtgaatat cgtcaactgg aagcgcagct
    10621 gcacgacgcc aacgaacgta acgacgggca cgccattgcg accattcata gcaagctgga
    10681 tgctattgac gcatggagta ttcgctcccg tgccgccagc ctgctgcacg gcctcggttt
    10741 cagcaatgaa caactggagc gcccggtaag tgatttctcc ggtggctggc gtatgcgtct
    10801 taaccttgcc caggcgctga tttgccgttc agacttgctg ctgctcgacg aaccgactaa
    10861 ccacctcgat ctcgatgccg ttatctggct ggaaaaatgg ttgaagagct atcagggcac
    10921 gctgatcctg atctctcacg accgcgactt cctcgatccg atcgttgata aaattattca
    10981 gatcgaacaa caaagcatgt tcgagtacac cggcaactac agttcgtttg aagtacagcg
    11041 cgccacccgt ctggcgcagc aacaagcgat gtacgaaagc cagcaggaac gcgtggcaca
    11101 tctgcaaagt tatatcgatc gtttccgtgc caaagccacc aaagcgaagc aggcccagag
    11161 ccgcattaag atgctggaac gtatggagct gattgccccc gcacacgtcg acaacccgtt
    11221 ccgctttagc ttccgcgcgc cggaaagcct gccaaatccg ttactgaaga tggaaaaggt
    11281 cagcgcaggc tatggcgatc gcattattct cgactcgatt aaattgaatc tggtccccgg
    11341 ctcgcgcatt ggtctgttag gccgcaacgg cgcaggtaaa tcgacattaa tcaaactgtt
    11401 agccggtgaa cttgcgccag tcagcggtga aattggtctg gcaaaaggga tcaagctcgg
    11461 ctacttcgcc cagcatcaac ttgaatacct gcgcgccgac gaatcaccta ttcaacatct
    11521 ggcacgttta gcgccgcagg agctggaaca aaaactgcgt gactacctcg gcggctttgg
    11581 tttcctgggc gataaagtaa ccgaagaaac gcgccgcttc tccggtgggg aaaaggcccg
    11641 cctggtgctg gcattaattg tctggcagcg tccgaatctg ctgctgctcg acgaaccgac
    11701 taaccacctt gacctcgaca tgcgtcaggc agtcaccgaa gcattaatcg agtttgaagg
    11761 cgcgctggtt gtcgtttcgc acgaccgtca tttgctgcgt tccaccactg acgatctcta
    11821 cctggttcac gatcgtaaag tcgaaccgtt cgacggcgat ctggaagatt atcaacagtg
    11881 gttgagcgac gtacaaaagc aggaaaacca gaccgacgaa gcgccaaaag agaacgcgaa
    11941 cagcgcccag gcacgtaaag atcagaagcg tcgggaagcg gagctgcgtg cgcaaaccca
    12001 gccgctgcgt aaagagattg cccgtctgga aaaagagatg gagaagctga acgcgcaact
    12061 ggcgcaggcg gaagagaaac tcggcgacag cgaactgtat gaccagagcc gtaaagcgga
    12121 gttgaccgcc tgcctgcaac agcaagccag cgccaaatcc ggcctggaag agtgcgaaat
    12181 ggcgtggctg gaagcccagg agcagcttga gcagatgctg ctggaaggcc aaagcaactg
    12241 atggcgcaga taacgacgac cgatgccaat gaatttagca gcagtgctga attcacccct
    12301 atgcgcggct ttagcaattg tcatctacaa actatgctgc cgcgtttgtt tcgtcgtcag
    12361 gtgaaattca ccccgtactg gcagcggctg gagttgcccg acggcgattt tgtcgatctc
    12421 gcgtggagtg aagaccccgc acaggcgaaa cataaaccgc gtttagtggt gtttcacggg
    12481 ctggaaggca gtctcaatag cccttacgcc cacggtctgg ttgaggcggc gcaaaagcgc
    12541 ggctggctgg gcgtggtgat gcattttcgc ggatgcagcg gtgaaccaaa ccgtatgcac
    12601 cgcatttacc attcgggcga aaccgaagac gccagttggt ttttacgctg gctgcaacgc
    12661 gaatttggtc atgcgccaac ggctgccgtc ggctattcgc tcggcggtaa tatgctggcc
    12721 tgtttgctgg caaaggaagg caatgatctc ccggttgatg cggcggtgat tgtctctgcg
    12781 ccgtttatgc tggaagcctg tagctatcat atggaaaagg gcttttcccg cgtttatcag
    12841 cgttacttgc tgaacctgtt aaaagccaat gccgcgcgca agctggcagc ctaccccgga
    12901 acgctgccga ttaatctcgc gcagttaaaa tcggtacgtc gcatccgtga atttgacgat
    12961 ctaatcaccg ccagaattca cggctacgcc gacgctatcg actattatcg tcagtgtagc
    13021 gccatgccga tgctgaaccg gatcgccaaa ccgacgctga ttattcacgc caaagacgat
    13081 ccgtttatgg atcatcaggt gatcccaaaa ccggaaagtc tcccctcgca ggtggagtat
    13141 caactgactg aacatggcgg tcatgttggc tttattggcg gtacattact tcatccgcaa
    13201 atgtggctgg agtcacgcat tcctgactgg ttaacaacgt atctggaggc gaaatcatgt
    13261 tgattccgtg gcaagacctc tcccccgaaa cgctggaaaa tttaattgaa agctttgtgt
    13321 tacgtgaagg caccgattat ggtgaacatg agcgtacact tgaacagaaa gtcgccgacg
    13381 tcaaacgcca gctacagtgc ggagaagcgg tgctggtatg gtcggaactg cacgaaacgg
    13441 tcaatattat gccgcgcagt cagtttcgcg aataacctgc aaccgtcggc ggacgagata
    13501 atagtgatag ctaagtcatg gagttactat gtctgccaaa catccggtca ttgcggtaac
    13561 aggatccagc ggcgcgggga ccaccaccac cagcctcgcg tttcgtaaaa tattcgcgca
    13621 gttaaatctg catgcagctg aggtggaagg cgacagtttt caccgttaca cccgcccgga
    13681 aatggacatg gcgatccgca aagcgcgcga cgccgggcgg catatcagct acttcggccc
    13741 cgaagcgaac gacttcggcc tgctggaaca aaccttcatt gaatacggtc agagcggcaa
    13801 agggaaatct cgcaaatatc tgcataccta cgacgaagcc gtaccctgga atcaggtacc
    13861 gggaacattc accccctggc aacctttacc agaacccact gatgtactgt tttatgaagg
    13921 tttacacggc ggcgtagtca cgccacagca taacgttgcg cagcatgtgg acttactggt
    13981 cggcgtagtg cctatcgtta accttgagtg gattcaaaaa ctgatccgcg acaccagcga
    14041 gcgcgggcac tcacgagaag cagtgatgga ctcggtagtg cgttcaatgg aagactatat
    14101 caactacatc acaccgcagt tttcccgcac ccatcttaac ttccagcgcg ttcccaccgt
    14161 cgacacttca aacccgttcg cggcaaaagg tatcccgtcg ctggatgaaa gctttgtggt
    14221 gatccatttt cgtaatctgg aagggatcga tttcccttgg ctgctggcga tgttgcaagg
    14281 ctcattcatt tcccacatca atacgttagt ggtacctggc ggcaaaatgg ggctggcaat
    14341 ggaattaatt atgctgctgc tggtgcaacg attgatggaa ggaaagaaaa tcgagtaact
    14401 ttgctattat gccggataaa atactatccg gcttcacaac gggatagtta agtcacgcgg
    14461 caaccacttc atacgagtga gtaatattca ccgctttttc cagcatcaac gccactgaac
    14521 aatatttctc ggcagagaga tcaaccgcac gcgcaaccgc tgcgtctttc aggtcgcgac
    14581 cggtgacgat aaaatgcaga ttaatgtgcg taaacaggcg tggtgcctct tcgcggcgtt
    14641 cagaggtcaa ttttacttca caatcgacca catcctgacg ccctttttgc aggatcgaaa
    14701 ccacatcgat ggcactgcaa ccacccgccg ccatcaacac catttccatc ggacttggtg
    14761 ctttatcgcc tgagttgccg tccattaaaa tctggtgacc agaggcggat tcgcccagaa
    14821 aagttaaccc ttcgacccac ttcactcgcg cttgcatttt tgctactcca ctgcgtcaat
    14881 tttcctgaca gagtacgcgt actaaccaaa tcgcgcaacg gaaggcgacc tgggtcatgc
    14941 tgaagcgaga caccaggaga cacaaagcga aagctatgct aaaacagtca ggatgctaca
    15001 gtaatacatt gatgtactgc atgtatgcaa aggacgtcac attaccgtgc agtacagttg
    15061 atagcccctt cccaggtagc gggaagcata tttcggcaat ccagagacag cggcgttatc
    15121 tggctctgga gaaagcttat aacagaggat aaccgcgcat ggtgcttggc aaaccgcaaa
    15181 cagacccgac tctcgaatgg ttcttgtctc attgccacat tcataagtac ccatccaaga
    15241 gcacgcttat tcaccagggt gaaaaagcgg aaacgctgta ctacatcgtt aaaggctctg
    15301 tggcagtgct gatcaaagac gaagagggta aagaaatgat cctctcctat ctgaatcagg
    15361 gtgattttat tggcgaactg ggcctgtttg aagagggcca ggaacgtagc gcatgggtac
    15421 gtgcgaaaac cgcctgtgaa gtggctgaaa tttcgtacaa aaaatttcgc caattgattc
    15481 aggtaaaccc ggacattctg atgcgtctgt ctgcacagat ggcgcgtcgt ctgcaagtca
    15541 cttcagagaa agtgggcaac ctggcgttcc tcgacgtgac gggccgcatt gcacagactc
    15601 tgctgaacct ggcaaaacaa ccagatgcta tgactcaccc ggacggtatg caaatcaaaa
    15661 ttacccgtca ggaaatcggt cagattgtcg gctgttctcg tgaaaccgtg ggacgcattc
    15721 tgaagatgct ggaagatcag aacctgatct ccgcacacgg taaaaccatc gtcgtttacg
    15781 gcactcgtta atcccgtcgg agtggcgcgt tacctggtag cgcgccattt tgtttccccc
    15841 gatgtggcgc agactgattt atcaccccga tatcaactat gcacttcgac aaacgctggt
    15901 gctatgtttg cccgtggccg ttgggttaat gcttggcgaa ttacgattcg gtctgctctt
    15961 ctccctcgtt cctgcctgtt gcaatattgc gggccttgat acgcctcata aacgtttttt
    16021 caaacactta atcattggtg cgtcgctgtt tgccacctgt agcttactga cacagctgct
    16081 actggtaaaa gatgttcccc tgcccttttt gctgaccgga ttaacgctgg tacttggcgt
    16141 cactgctgag ctggggccat tgcacgcaaa attgcttcct gcatcgctgc tcgccgccat
    16201 ttttaccctc agtctggcgg gatacatgcc ggtctgggaa ccattgctca tctatgcgtt
    16261 gggcactctc tggtacggat tgtttaactg gttttggttc tggatctggc gcgaacaacc
    16321 gctgcgcgag tcactaagtc tgctgtaccg tgaactggca gattattgtg aagccaaata
    16381 cagcctgctt acccagcaca ccgatcctga aaaagcgctg ccaccgctgc tggtgcgcca
    16441 gcaaaaagcg gtcgatttaa ttactcagtg ctatcagcaa atgcatatgc tttccgcgca
    16501 aaacaatacc gattacaagc ggatgctgcg tattttccag gaggcgctgg atttgcagga
    16561 acatatttcg gtcagtttgc atcagccgga agaggtgcaa aaactggtcg agcgtagcca
    16621 tgcggaagaa gttatccgct ggaatgcgca aaccgtcgcc gctcgcctgc gcgtgctggc
    16681 tgatgacatt ctttaccatc gcctgccaac gcgttttacg atggaaaagc aaattggcgc
    16741 actggaaaaa atcgcccgcc agcatccgga taatccggtt gggcaattct gctactggca
    16801 tttcagccgc atcgcccgcg tgctgcgcac ccaaaaaccg ctctatgccc gtgacttact
    16861 ggccgataaa cagcggcgaa tgccgttgct tccggcactg aaaagttatc tgtcactaaa
    16921 gtctccggcg ctacgcaatg ccggacgact cagtgtgatg ttaagcgttg ccagcctgat
    16981 gggcaccgtg ctgcatctgc cgaagtcgta ctggatcctg atgacggtat tgctggtgac
    17041 acaaaatggc tatggcgcaa cccgtctgag gattgtgaat cgctccgtgg gaaccgtggt
    17101 cgggttaatc attgcgggcg tagcgctgca ctttaaaatt cccgaaggtt acaccctgac
    17161 gttgatgctg attaccaccc tcgccagcta cctgatattg cgcaaaaact acggctgggc
    17221 aacggtcggt tttactatta ccgcagtgta taccctgcaa ctgttgtggc tgaacggtga
    17281 gcagtacatc cttccacgtc ttatcgatac cattattggt tgtttgattg ctttcggtgg
    17341 taccgtctgg ctgtggccgc agtggcagag cgggttattg cgtaaaaacg cccatgatgc
    17401 tttagaagcc tatcaggaag cgattcgctt gattctgagc gaggacccgc aaccaacgcc
    17461 actggcctgg cagagaatgc gggtaaatca ggcgcataac actctgtata actcattgaa
    17521 tcaggcgatg caggaaccgg cattcaacag ccattatctg gcagatatga aactgtgggt
    17581 aacgcacagc cagtttattg ttgagcatat taatgccatg accacgctgg cgcgggaaca
    17641 ccgggcattg ccacctgaac tggcacaaga gtatttacag tcttgtgaaa tcgccattca
    17701 gcgttgtcag cagcgactgg agtatgacga accgggtagt tctggcgatg ccaatatcat
    17761 ggatgcgccg gagatgcagc cgcacgaagg cgcggcaggt acgctggagc agcatttaca
    17821 gcgggttatt ggccatctga acaccatgca caccatttcg tcgatggcat ggcgtcagcg
    17881 accgcatcac gggatttggc tgagtcgcaa gttgcgggat tcgaaggcgt aatacaggct
    17941 aaaccatcgg ataaggcatt cacgccgcat ccgacatatt ttgcctgatg cgtcttattt
    18001 acgccccaac caccttcgcc accgcgtggg cgaaacgttg catcccctca tcgatatccg
    18061 catcttccac caccagcgac ggtgctaaac gcatcacatc cggtccggca ttcagcacca
    18121 ttacgccaac ctctgcaccc gcatacagga aatcacgcgc ccgacctttg tagtgtggtt
    18181 tcagctccgc gccaattaac agccccatac cgcgaatatc gctaaacaca tcgtactgct
    18241 gatcgatctt ctgcaaatgg tcaacaaaac gctggcgttt cgcctgaatg ccttccagca
    18301 cttcaggggt attgatgata tcaaacgccg cgcccgctac tgcacaggcc agaggattac
    18361 cgccgtaggt ggaaccgtga gaaccaggat gaaacgcaga agcaatttcc gccgtggtca
    18421 gcatggcgct aatcgggaag ccgccgccta acgctttcgc agaagtcaga atatccggcg
    18481 tcacaccgta gtacctgtaa gcaaacaaat cgccagtccg ccccatcccg cactgcactt
    18541 catcaaacac caataatacc tgatgttgat cgcacagctc gcgcaagccc tgcaaaaact
    18601 ctggcgtcgc tgccgtcaca ccgccctcgc cctggatcgg ctcaaccacc accgcacagg
    18661 tgtgatcatc catcaccgct ttcactgcat ggagatcgtt aaagggaacg tggatgatgt
    18721 ctgccggttg cggcccaaag ccgtcggaat attttggctg cccacccacc gaaacggtaa
    18781 acagcgagcg accatgaaaa gcgttatgga aggcaataat tttggttttg aacgggctat
    18841 gacgcacaca ggcgtaatgg cgtgccagtt taaaggcggt ttcgttagct tccgtgccgg
    18901 agttcataaa caccacgcgt tcggcaaacg tcgcctcaat cagtttgcgc ccaagacgca
    18961 gcgccggttc attggtgaaa acgttactga tatgccacag agtttcgccc tgggttttta
    19021 acgcgttcac cagcgcagga tggcaatggc ccaacgccgt aactgcaatg ccacccgcga
    19081 aatcgacata ctccttgcct tgctgatccc agattcggct gccctgacct tttaccggaa
    19141 taaactctgc cggagcataa atcggcagga tcacttcatc gaaagtcgcg cgtgtaattg
    19201 ctgtttgttc aattgccatc tcatgatcac cctgttacgc ataaataaat atgaaattat
    19261 aatcacaaaa tatgcataaa aaatcactga atggcaatcg gaaatcagag atgcaggaaa
    19321 ttagccagca gttgatgtcc ttgttcgcta agaatacttt ctggatggaa ctgcacacct
    19381 tccagatccc actggcgatg gcgaatcccc ataatctcgc gggtttcgct ccaggccgtc
    19441 acgtcaaagc acgctggtaa tgagtcaggt tccaccacca gcgaatggta gcgtgtcacg
    19501 gtaagtggat ttgccagccc ccgaaatacg ccctcaccgc tatgtgtaat cggcgaggtt
    19561 ttgccgtgca tgacctttgc ggcgcgcaca actttgccgc caaatgcctg cgccattgcc
    19621 tgatgaccga ggcagacgcc aagaatcggc aagcgcccgg catagtggcg aataacgtca
    19681 agggagatcc cggcttcatc tggcgtacag gggccaggtg agatgacaat tttttgtggt
    19741 ttaagggcgt cgatatccgc cagcgtcaac gcatcgttgc gcttaaccag cacatccgcc
    19801 cccagttcac aaaagtactg gtagaggttc caggtaaaag aatcgtagtt atctataagc
    19861 aggatcatgg cggctccggt acgaaagaac cgcactattt tactcagatt ccccggcttc
    19921 gcttaccact ttgctaaata tcatctgcag tgcggtgaga tcccccattg ccccactctg
    19981 atttgcctga ttccaagcct ctttttcgat accctgccag ctcagttgat atccggcatg
    20041 aatcgccagt tgctcgaaga agatccgctg tgccagacca cttcccaccc ggaagggatg
    20101 cagcacgtcg atttcacaat agtaatgcgc cagccgctcg acgaacttcg ctttctccag
    20161 gccaaccaga taaccttctt cctccagatc ctgcatcagg gcattgccct ctttttcgat
    20221 ataagcaaag tggcagaacg gcgtatcgcc ctgataaata tcaacttcac gcagttgccc
    20281 tgcccagtcg aaaatatcct ggtacagctg gcgatggata gtgcgcaaat gcggtaaacc
    20341 gcgcaccagc ggaccaagct caatggtcgc agcacgcagc gccgtcattt cgtaagcggc
    20401 ctgttccagc cgctgctgct ggcggatgtt cagccggtta cgcatgatat caaggccagg
    20461 ataaagatac ggatcgcgcc cttcgccgaa tttatcgctc atagtgcctc ctcagctctt
    20521 caaggcgcgc taaagcctct gcggcagtaa gagtgactaa aggcatctcg acgccttcaa
    20581 gacggcgact ggcctggaaa ttacgattac gctgaagctc ccagagacgg gacttttgct
    20641 tatcggtgag tttcttcact tgatgcctcc ctgaacgtgt ccatttgcca caagtataag
    20701 cggcaaatcc gggttacgcc gggagagcag aagcgcgggc aagattgccc gcgcgagaaa
    20761 tcattacggc aggactttag cggaaaggat aactaccggt tttgacggca cattctggta
    20821 cggaccaaca tcatgagtcg gcacctggga aatcttatcg gcaacgtcca tgcctttcac
    20881 cactttacca aataccgcgt aaccgaaatc acgctgacca tggtcaagga aggcgttatc
    20941 ggcaacgttg ataaagaact ggctggtggc gctgtctttg tcagcggtac gcgccatcgc
    21001 gatggtgcca cgcgtgttgc gcaggccgtt atcggcttca tttttgattg gcgggtttgg
    21061 ttttttctgc tgcatctgct cggtgaaacc gccgccctga atcataaagc caggaatgac
    21121 gcggtgaaag gtagtgttgt tataaaaacc gctgttcaca taatcgacaa agttttgcac
    21181 agacactggc gctttttgtt tatccagctc cagttcgatg ttaccagctg aggttgtcaa
    21241 caatacgtgc gggtcccctt tcgctgccat tgctgcggga gaaagagcag aaagagcgaa
    21301 aacagcagcc atcgccgcca gggtcgattt gaacatgaga tttccttaac aaacagagca
    21361 gaaaaaaagc aagtgaagag attctaaata gccttcaaga acaaggccat ccctttacct
    21421 aattttacgt atctgaaata tatgtaacct gacgatcact ttttattccg taatcaaaat
    21481 cacctcttaa aatgcaattt agcaaccgat tgcaataaaa catttaaaca gatcacaaaa
    21541 tcacctaaaa tcgcccgctc gcggcatatc ccgctatgcc attttatttt tgaacgcagg
    21601 ccaatcatga ctaacagcaa tcgcatcaag ctcacatgga ttagctttct ctcctacgca
    21661 ctgactggtg cgttggttat tgtcaccggg atggtgatgg gaaatatcgc cgattatttc
    21721 aatctgcctg tttccagtat gagtaacacc ttcaccttcc tcaacgccgg cattttaatc
    21781 tctatcttcc tcaacgcctg gctgatggaa atcgtcccgt tgaaaacgca gttacgtttt
    21841 ggctttctcc tgatggtgct ggcggttgcc ggtttgatgt tcagccacag cctggcgctg
    21901 ttctcgacgg cgatgttcat tctcggggtg gtcagcggca ttactatgtc gattggtaca
    21961 ttcctgataa cacaaatgta tgaagggcgt cagcgcggtt cccgcctgtt atttactgac
    22021 tccttcttca gtatggctgg gatgattttc ccaatgatcg ccgcgtttct actggcgcgc
    22081 agcattgagt ggtactgggt ttatgcctgc atcgggctgg tgtatgtcgc tatttttatt
    22141 ctgaccttca gctgtgagtt cccggcactg ggcaaacgtg caccaaaaac ggatgctccg
    22201 gtagagaaag aaaagtgggg gatcggcgta ctgtttctct ctgttgcggc gctgtgctac
    22261 atcctcggtc agttaggttt tatctcctgg gtgcctgagt atgccaaagg cctgggcatg
    22321 agcctgaacg acgcgggcac gctggtgagt aacttctgga tgtcatacat ggtcggcatg
    22381 tgggcgttca gctttattct tcgcttcttt gatttgcaac gcattctgac cgtactggct
    22441 ggtctggctg cgattctgat gtacgtcttt aacaccggaa caccagcaca tatggcgtgg
    22501 tcaattctcg ctctgggctt cttctccagc gcgatctata ccaccatcat caccctgggt
    22561 tcacagcaga ccaaagtacc gtcgccaaaa ctggttaact ttgtcctgac ctgcggaacc
    22621 atcggtacta tgttgacctt tgtggttacc ggcccgattg ttgaacatag cggtccgcag
    22681 gcggcactgc ttacagcaaa cggtctgtac gctgtcgtct ttgtgatgtg cttcctgtta
    22741 ggtttcgtca gccgtcaccg tcagcataac accctgacct ctcattaatt gctaatgccg
    22801 gacggcacta tcgtcgtccg gccttttcct ctcttccccc gctacgtgca tctatttcta
    22861 taaacccgtt cattttgtcc attttttgca caaacatgaa atgtcagaca attccgtgac
    22921 ttaagaaaat ttatacaaat cagcaatata ccccttaagg agtatataaa ggtgaatttg
    22981 atttacatca ataagcgggg ttgctgaatc gttaaggtag gcggtaatag aaaagaaatc
    23041 gaggcaaaaa tgagcaaagt cagactcgca attatcggta acggtatggt cggccatcgc
    23101 tttatcgaag atcttcttga taaatctgat gcggccaact ttgatattac cgttttctgt
    23161 gaagaaccgc gcatcgctta tgaccgcgta cacctctcgt cttacttctc tcaccacacc
    23221 gccgaagagc tgtcgctggt gcgcgaaggc ttctacgaga aacacggcat caaagttctg
    23281 gtcggcgaac gcgctatcac catcaaccgt caggagaagg tgattcactc cagcgccgga
    23341 cgtaccgttt tttatgacaa gctgatcatg gcaaccggtt cctacccgtg gatcccacca
    23401 atcaaaggtt ctgatactca ggactgcttt gtctatcgca ccattgaaga cctcaacgcc
    23461 attgaatcct gcgcccgtcg cagcaaacgc ggtgccgttg ttggtggcgg cctgttaggt
    23521 ctggaagccg ctggcgcgct gaaaaactta ggtattgaaa cccacgttat cgaatttgcc
    23581 cctatgctga tggcggaaca gcttgatcag atgggtggcg agcagctgcg tcgcaaaatc
    23641 gaaagtatgg gcgtgcgcgt tcacaccagc aaaaacaccc ttgagattgt gcaggaaggt
    23701 gtcgaagcgc gtaaaaccat gcgttttgcc gacggcagcg aactggaagt cgactttatc
    23761 gtcttctcta ccggtatccg tccgcgcgat aagctggcaa cccagtgtgg tctggacgtt
    23821 gctccgcgtg gggggattgt cattaatgat tcctgccaga cttccgatcc ggatatctac
    23881 gccatcggtg aatgcgcaag ctggaacaac cgtgtatttg gtctggtagc accaggctac
    23941 aaaatggcgc aggtcgccgt tgaccatatt ctgggtagcg aaaacgcctt tgaaggtgct
    24001 gaccttagcg ccaagctgaa actgctgggc gtagacgtag gtggtattgg tgatgcgcac
    24061 ggtcgcacgc ctggcgcacg tagctatgtt tacctcgacg aaagtaaaga gatctacaaa
    24121 cgcctgattg tcagcgaaga caacaaaacc ctgctcggtg cggtactggt gggcgatacc
    24181 agcgactacg gcaacctgct gcaactggtg ctgaacgcta tcgagctgcc ggaaaacccg
    24241 gattctctga ttctgccggc gcactcgggt agcggtaagc cgtctatcgg tgttgataaa
    24301 ctgccggaca gcgcgcaaat ctgctcctgc ttcgacgtca ctaaaggtga tctgattgct
    24361 gccatcaaca aaggctgcca cacagttgcg gcgctgaaag ctgaaaccaa agcgggtact
    24421 ggctgcggtg gctgtatccc gctggtcact caggtactga acgcggaact ggcgaaacag
    24481 ggcatcgaag ttaacaacaa cctgtgcgaa cactttgctt attcgcgtca ggaactgttc
    24541 catttgatcc gcgttgaagg cattaaaacc ttcgaagaac tgctggcgaa acacggcaaa
    24601 ggctacggtt gtgaagtttg taaaccaacc gtcggttcgc tgctggcctc ctgctggaac
    24661 gaatacattc tgaagccgga acatactccg ctgcaggatt ctaacgacaa cttcctcgct
    24721 aacatccaga aagacggcac ctactcggtg atcccgcgtt ctccgggcgg tgaaatcacc
    24781 ccggaagggc tgatggcagt gggtcgtatc gcgcgtaaat ttaatctcta caccaaaatc
    24841 accggctccc agcgcctggc gatgtttggc gcacagaaag gcgatctgcc ggaaatctgg
    24901 cgtcagctga ttgaagctgg cttcgaaacc ggtcatgcct atgcgaaagc actgcgtatg
    24961 gcgaaaacct gcgtgggtag cacctggtgc cgctacggtg ttggcgatag cgtcggcctc
    25021 ggcgtggaac tggaaaaccg ctacaaaggc atccgtacgc cgcacaaaat gaagttcggt
    25081 gtctccggct gtacccgtga atgttcagaa gctcagggta aagacgtggg tattatcgcc
    25141 actgaaaaag gctggaacct gtatgtttgc ggtaacggcg gcatgaaacc gcgtcatgcg
    25201 gatctgctgg cagcggatat cgatcgcgaa acgctgatca aatatctcga ccgcttcatg
    25261 atgttctaca tccgtaccgc cgacaaactg acgcgtaccg cgccgtggtt agaaaacctc
    25321 gaaggcggca tcgattacct gaaagcggtg atcattgacg acaaactggg gctgaacgct
    25381 catctggaag aagagatggc gcgcctgcgt gaagcggtag tgtgtgagtg gactgaaacg
    25441 gtcaatacac cgtccgcgca gactcgcttc aaacacttca tcaacagcga caagcgtgac
    25501 ccgaacgtgc agatggtgcc agagcgcgaa cagcaccgtc cggcaacgcc gtatgaacgt
    25561 atcccggtaa ctctggtgga ggacaacgca tgagccagtg gaaagacatc tgcaaaatcg
    25621 atgacatcct gcctgaaacc ggcgtctgcg cgctgttagg tgacgagcag gtagcgattt
    25681 tccgcccgta tcacagcgac caggtgtttg cgatcagcaa cattgatccg ttcttcgagt
    25741 ccagcgtgct atcacgcggg ctgattgcgg aacaccaggg cgagctgtgg gtcgccagcc
    25801 cgctgaaaaa acagcgtttt cgcttaagtg atggcttgtg catggaagac gaacagtttt
    25861 ccgtcaaaca ttacgaagca cgagtgaaag acggcgtggt gcagctgcgc ggttaatgtt
    25921 ttaacgggag gcgcaatgcc tccccttttt gcatggtcct gtaatgacct tcggtatatt
    25981 gcaggacatt ttttaaactt tttgttttat tttttgtttt tattttttaa aggataatca
    26041 aatgtttaca gacactatta ataagtgtgc ggctaatgct gcgcgcattg cacgcctgtc
    26101 ggcaaataac ccgctcggct tttgggtcag ctccgccatg gcgggcgcat atgtgggtct
    26161 tgggatcatc ctgattttca cgctcggtaa tttgctcgac ccgtccgtac gtccgctggt
    26221 gatgggcgcg acctttggta tcgccttaac gctggtgatt atcgccggtt ctgaactgtt
    26281 caccggacac accatgttcc tcacctttgg ggtaaaagcg ggcagcatca gccacgggca
    26341 aatgtgggca atcctgccgc aaacctggct gggtaacctg gtcggttccg tcttcgttgc
    26401 catgctctat agctggggcg gcggtagcct gctgccggta gataccagca tcgttcactc
    26461 cgtcgcgctg gctaaaacca ctgcaccggc aatggtactg ttcttcaaag gtgcattgtg
    26521 taactggctg gtttgcctgg caatctggat ggcgctgcgc actgaagggg cggcgaaatt
    26581 tatcgctatc tggtggtgtc tgctggcatt tatcgcgtcc ggctacgagc actctatcgc
    26641 taacatgacg ctgttcgcgc tctcctggtt cggcaaccac agcgaagcct acacgctggc
    26701 gggtattggt cataacctgc tgtgggtgac gctgggtaat actttatcag gtgccgtatt
    26761 catgggattg ggttattggt atgctacgcc gaaagcgaat cgtccggttg cggacaaatt
    26821 taatcaaact gaaacggctg ccggttaatt actaaggggt ttttacgtgg atcatttgcc
    26881 tatattttgc caattacgcg atcgcgactg tctgattgtc ggcggtggtg atgtcgcgga
    26941 acgcaaagca aggttgctgt tagacgcagg cgctcgctta acggtgaatg cattagcgtt
    27001 tattccacag ttcaccgcat gggcagatgc aggcatgtta accctcgtcg aagggccatt
    27061 tgatgaaagc cttctcgaca cctgctggct ggcgattgca gcgacggatg atgacacgct
    27121 taaccagcgc gtcagcgaag ccgctgaagc tcgtcgcatc ttctgtaacg tagtcgatgc
    27181 gccgaaagcc gccagcttta ttatgccgtc gattattgac cgctcaccgc taatggtagc
    27241 ggtctcctct ggcggcacct ctccggttct ggcgcgcctg ttgcgcgaaa aactcgaatc
    27301 actgctgccg ttgcatctgg gccaggtggc gaaatatgcc gggcaattac gcgggcgagt
    27361 gaaacaacag ttcgccacga tgagtgagcg tcgccgtttc tgggagaaat tgttcgttaa
    27421 cgaccgcctg gcgcagtcgc tggcaaacaa cgatcagaaa gccattactg aaacgaccga
    27481 acagttaatc aacgaaccgc tcgaccatcg cggtgaagtg gtgctggttg gtgcaggtcc
    27541 gggcgatgcc gggctgctga cgctgaaagg actgcaacaa attcagcagg cagatgtggt
    27601 ggtctacgac cgtctggttt ctgacgatat tatgaatctg gtacgccgcg atgctgatcg
    27661 cgttttcgtc ggcaaacgcg cgggatacca ctgtgtaccg caggaagaga ttaaccagat
    27721 cctgctgcgg gaagcgcaaa aaggcaaacg cgtggtgcgg ctgaaaggtg gcgatccgtt
    27781 tatttttggc cgtggtggcg aagagctgga aacactgtgc aacgcgggta ttccgttctc
    27841 ggtggttccg ggtattaccg cagcttctgg ttgctctgcc tattcgggta ttccactcac
    27901 gcatcgcgat tatgcccaga gcgtacgctt aattaccgga cacttaaaaa ccggtggcga
    27961 gctggactgg gaaaacctgg cggcagaaaa acagacgctg gtgttctata tggggttgaa
    28021 tcaggccgcg actattcagc aaaagctgat tgaacacggt atgcctggcg aaatgccggt
    28081 ggcaattgtc gaaaacggaa cggcagtcac gcagcgcgtg attgacggta cgctcacgca
    28141 actcggtgaa cttgctcagc aaatgaacag tccatcgcta attattattg gtcgggttgt
    28201 tggcctgcgc gataaactga actggttctc caatcattaa tttaacccgg gccagagaat
    28261 tctggccttc ttaatgactc tttttatata ttcagcaaat aaaacataac cccttaataa
    28321 atattatctg ctaatgggtt atattcaccg ttacagcgac ttacaaaatt aagtcattcg
    28381 ctcactgacg ccaattaaat attcctgtat cgttcttaac gagcagtctg cttatattca
    28441 tataatcaat gaatattaat taatatataa tacataggga atgtaaatga acaaatttat
    28501 taaagttgca ctggtaggtg cagtactggc tacgttaact gcatgtactg gtcatattga
    28561 aaaccgtgat aagaactgct cttacgacta cctgctgcac ccggcaattt ctatttctaa
    28621 aatcattggc ggttgcggtc ctactgcaca gtaagtctct gacagaaatc ggctaacacc
    28681 ggtttaattg cccgacgccc tctggatttt ccgagggcgt atttgtttct atgtccttct
    28741 tgcccccgcc cgtaaaaata ttttcacttt aaattcaatt ttataactac atccattagc
    28801 cacagcgcga tcctgtgcga aattttgtga tcttcctcca cattacacaa catcatatgt
    28861 tgttatattc atcatgcatt gtcatgttgc cttttaaatg actgcaaact ctcccctaca
    28921 acgtattgga caagaaaaag gtatcgctat gggaagccag gaactccaac gcaagctcgg
    28981 attttgggcc gttcttgcaa tcgccgtcgg gacaaccgtc ggctccggta tttttgtatc
    29041 tgtgggtgaa gtggcaaaag cagcgggcac gccgtggctt acggtgctcg cgtttgtcat
    29101 tggcgggtta attgtgatcc cgcaaatgtg cgtctatgcg gaactatcca ccgcttatcc
    29161 ggaaaatggc gcagattatg tttatctgaa aaatgccgga agccgaccgc tggctttcct
    29221 ctccggctgg gccagcttct gggccaacga tgcgccgtca ttgtcgatta tggcgctggc
    29281 gattgtcagc aatcttggct ttttaacgcc tatcgatccg ttgctcggta aatttatcgc
    29341 cgccggatta attatcgcct ttatgttgct acacctgcgc tccgttgaag gcggcgcagc
    29401 gtttcagacg ctaattacca tcgccaaaat tatcccgttc actatcgtca ttggccttgg
    29461 gatcttctgg tttaaagcgg agaattttgc cgcccttacc accactgcga ttggcgcaac
    29521 gggcagcttt atggcgctgc tggcggggat ctctgccacc agttggtcgt ataccggcat
    29581 ggcctctatc tgttatatga ccggcgaaat taaaaacccc ggaaaaacca tgccacgagc
    29641 gctgattggt tcctgtctgc tggttctggt gctctacacc ctgctggcgc tggtgatttc
    29701 cggcctgatg cccttcgaca aactcgccaa ttctgaaacg ccgatttccg acgccctgac
    29761 ctagatcccc gcactcggca gcaccgctgg gatctttgtt gccatcacgg cgatgatcgt
    29821 cattcttggt tcgctttcca gctgcgtgat gtaccagccg cggctggaat acgcgatggc
    29881 gaaagacaac ctgttcttta aatgcttcgg ccatgtgcat ccgaaataca acacgccgga
    29941 tgtctccatc atcctgcaag gggcgctggg gatcttcttc atcttcgttt ccgatctcac
    30001 cagcctgctg ggttatttca ccctggtgat gtgtttcaaa aataccctca ccttcggctc
    30061 catcatctgg tgtcgtaaac gcgacgatta caaaccgctg tggcgtactc cggctttcgg
    30121 gctgatgacc accctcgcca ttgcgtcaag cctcattctg gtcgcctcaa cctttgtctg
    30181 ggcaccgatt cccggcctta tctgcgccgt catcgttatt gctactggtc tgcctgctta
    30241 cgccttctgg gcgaagcgta gccgccagct caacgctttg tcgtaatgtt atctgaagaa
    30301 aataaaatgt tggatattga taaaagcacc gtggactttc tggtcaccga aaatatggtt
    30361 caggaagtgg aaaaagttct cagccatgac gttccgctgg tgcacgccat cgtggaagag
    30421 atggtgaagc gcgacattga tcgtatttat ttcgttgcct gcggctcgcc actcaacgcg
    30481 gcgcaaacgg cgaaacatct ggcggatcgc ttttccgatc ttcaggtcta cgccatttcc
    30541 ggctgggagt tctgcgataa caccccgtat cgcctcgacg atcgttgcgc ggtgattggc
    30601 gtttctgact acggtaaaac ggaagaggta atcaaagcgc tggagctggg ccgggcctgc
    30661 ggcgcactca ctgcggcgtt caccaaacgc gcggacagcc cgattacctc ggcggcggaa
    30721 tttagcattg attatcaggc cgactgtatc tgggaaattc acctgctgct ctgctacagc
    30781 gtggtgctgg agatgatcac cctcctcgcg ccgaacgcgg aaatcggcaa gatcaaaaac
    30841 gatctcaagc agttgccgaa tgcgctcggt catctggtac gcacctggga agaaaaaggc
    30901 cgccagcttg gtgaactggc cagccagtgg ccgatgattt ataccgttgc tgcgggtccg
    30961 ctgcgtccgc tgggttacaa agaaggcatt gtaacgctga tggaatttac ctggacgcac
    31021 ggctgcgtga ttgagagcgg agagttccgc catggcccgc tggagattgt cgaaccgggc
    31081 gttccgttcc tgttcctgct cggcaatgat gaaagtcgcc acaccaccga acgcgccatt
    31141 aactttgtta aacagcgtac tgacaacgtg atcgtcatcg attacgccga aatttcgcaa
    31201 gggctgcacc cgtggctggc accgttcctg atgttcgtgc caatggagtg gctctgctac
    31261 tacctgtcta tttacaaaga tcacaacccg gatgaacgcc gctattacgg tggtctggtg
    31321 gaatattaat ccctctcccg gcccggcaac gggccggatt tacgcaagga gttacccgat
    31381 gaaaacaggt atgtttacct gcggccacca gcggctgcct attgaacacg catttcgtga
    31441 tgcaagcgag ctgggttacg acggcatcga aatttggggc ggtcgcccgc acgcgttcgc
    31501 gccggactta aaagcgggcg gcatcaaaca aatcaaggcg ctggcgcaga cgtatcagat
    31561 gccgattatc ggttacacgc cagaaaccaa cggctatccg tataacatga tgctgggcga
    31621 tgaacatatg cgtcgtgaaa gcctggacat gatcaagctg gcgatatata tggcaaaaga
    31681 gatgaacgcg ggttatacgc tgatttctgc ggcccacgcg ggctatctca cgccgcctaa
    31741 cgttatctgg gggcggctgg cagagaactt aagcgaactg tgtgagtacg cggaaaacat
    31801 cggcatggat ctgatcctcg aaccgttaac gccgtatgaa tcgaacgtcg tgtgtaatgc
    31861 taatgatgtg cttcatgcgc tggcgctggt gccttcgccg cgcctgttca gcatggtcga
    31921 catttgcgcg ccgtatgtcc aggcggaacc ggtgatgagt tatttcgaca aactgggcga
    31981 taaattacgt catctgcata ttgtcgacag cgacggggcc agcgacacgc attacattcc
    32041 tggagaaggc aaaatgccgc tgcgggaact gatgcgcgat attattgatc ggggctatga
    32101 gggttactgt acggtggagc tggtgacgat gtatatgaac gagcccagac tctatgcccg
    32161 ccaggcgctg gaacgctttc gcgcgctgct gccggaggat gagagatgaa aaccctagcg
    32221 acaatcggcg ataactgcgt cgatatctac ccgcaactga ataaagcgtt ttctggcggt
    32281 aatgcggtca atgtggcggt gtactgcact cgctacggca tacagccggg atgcattacc
    32341 tgggtgggtg acgatgacta cggcacaaag ctgaagcagg atctcgcccg catgggcgtc
    32401 gatatcagcc atgtccatac gaaacacggc gttaccgcac aaactcaggt ggaactgcac
    32461 gacaatgatc gcgtttttgg cgactacacc gaaggcgtga tggccgactt tgccctgagt
    32521 gaagaggatt acgcctggct ggcgcagtat gacattgtgc acgcggcaat ctggggacat
    32581 gcggaagacg cattcccaca gctgcacgct gcgggcaaac ttaccgcttt cgacttctcc
    32641 gacaagtggg acagcccgct ctggcagaca ctggtgccgc atctcgattt tgcctttgcc
    32701 tccgcaccgc aagaagacga agcgctgcgt ctgaagatga aagcgattgt tgcccgtggc
    32761 gcaggaacag tgattgtcac gctgggtgaa aacggcagca ttgcctggga tggcgcgcag
    32821 ttctggcgtc aggctcctga accggtgacg gttatcgaca ccatgggtgc cggagattcg
    32881 ttcattgccg gattcctttg cggctggtct gcggggatga cattaccgca ggcgatggcg
    32941 cagggaacgg cgtgcgcggc gaaaaccatt cagtaccacg gtgcctggta ggtataacgt
    33001 tggcgtgagc atcttcacgc caacgtgctg ttacttgccg gaaaacgacc ctataatccg
    33061 agtaattcat tctttatttc agggtcgatt atgtcagcta cggaccgcta ctctcatcaa
    33121 ctcctctacg ctaccgtccg ccagcggctg ctggatgata tcgcgcaggg ggtttaccag
    33181 gccgggcaac agatccctac cgaaaacgag ctttgtacac aatataacgt cagccgcatt
    33241 accattcgca aagccatcag cgacttagtg gcagacggcg tactgatccg ctggcaggga
    33301 aaaggcacct ttgtacaaag ccagaaagtt gaaaacgccc tgcttactgt cagtggtttt
    33361 accgattttg gcgtctcaca aggcaaggcg acgaaagaga aagtgatcga acaggaacgg
    33421 gtcagcgccg cgccgttttg cgaaaagctg aacatccccg gaaacagcga agtgttccat
    33481 ctctgccggg tgatgtatct cgataaagag ccgctgttta ttgatagttc atggatcccg
    33541 ctgtcgcgtt atcctgactt tgatgagatt tacgtcgaag gaagctccac ctatcagtta
    33601 tttcaggagc gttttgacac gcgagtggtc agcgacaaaa agaccatcga tatctttgcc
    33661 gccacccgcc cgcaggcaaa atggctgaaa tgcgaactgg gcgaaccgtt gtttcgcatc
    33721 agcaaaatcg cctttgatca gaatgacaaa ccggtgcacg tctccgaact cttctgccgc
    33781 gccaatcgca tcaccttaac gattgataat aaaagacatt aaccgtaggc cggataagat
    33841 gcgccagcat cgcatccggc gatgctggcg cactgaattt tacatcccgc acgttcccct
    33901 caccctaacc ctctccccaa aggggcgagg ggagcgttca gtgcggaatt tagcttgatt
    33961 gccgaacggt gctatccagc attaaatact cgcaatactc tcacgcaaaa tccgcagcac
    34021 cgtctcttca ccgctgcgat tcgggttaat gcgaatcgca caatgttcga gttgtggatt
    34081 cgcctggcga aacgttcctg aaagtcgata gaagagcggc gggatttcat atttcgactc
    34141 tgcaccgacc gggtaaggca acgcgccacg cttttgcgct tcttccagca ccctggcggc
    34201 aatcagctga tgaaactcga caatcaacac cttcgactgc gcattggcaa tgacggcgct
    34261 tttcacttcc gccaccgcgc cgccgttaag caaagccagc aaccgttcgg atactcccgc
    34321 ctgcaccgca tgcatcactg gcgcgaacac cagaccacgc aagacttcca gcgcctgtga
    34381 gccctggatc tggctaccgc cggagtaaag cgtggcgcga atccgactaa taacatcggc
    34441 atcgccaacc actgcaccaa cgccctccgg cccaaacagc ttgaagcagg agaatgtcga
    34501 gacattcgcg ccgcattcac agccgattcg cgctaccttc atcaccgcat agttatcatc
    34561 ggttaacgct ggaacacctg ccgcgcgcaa cgttgccagc acatctgcca gcacatagct
    34621 gtcctgcggc tgctggcgcg tatgctgcac cagcgcggca tcaggttgtt gctcgtcgac
    34681 gacctgcttc agtgccaaca ggtcattgaa atcagcagta ataagcgtca gccccatctg
    34741 ctcaataata acccgtgtcg tcgggtaaac aggcgcgtca tgcaccagaa gacgctgccc
    34801 cggtttgagc aaagccgcca gcccggcgcg aatcgcgcca gtccccgcgc cctgcaccag
    34861 cgccgcggcc tgtgcgtgaa atgcatcagc cagcacctgc tccacccgtt gggtaatacg
    34921 cggttgattc agacctggag ttaaccctaa atcaccgcca gcaagaaact cgctgcctgg
    34981 aaaatggcgg caaatagtgt ccaccagcgc aaactgcttt tgctgcgctt cagcaagagt
    35041 caggctttgc aaaggaaacg tcttcatagg agccttcctt aagccggaac gaacaggcca
    35101 agccagtaaa gaacattcag cagaataccg gtgatcatta ctgccacgac cggtgccgcc
    35161 attttctgta ccggacggcc cagggattcg ttgaggaagt aaatagcaac cgcgatagag
    35221 aatccggtat aacccgccat cttaattgct gcgaaaatcg aaccgaccag cagcgccatt
    35281 tccatcagca tattcatggc gttacggatg ttatccgacg cattacgcac cgacgggtag
    35341 cgtcccagcc atttgccgat cgaacgaagc agcaagactt ccgccgaaat aaccactgcg
    35401 cctaataccg ctgcaaccat cggattcggc gagagatagc ccaccgcata aacaaaggta
    35461 aagcccgcaa ctgcatacac acccgttgct aaggcggtgg tggcaatcaa cggcacaaac
    35521 cccagtccgc gcataaattc tgccagagcc gcctgattaa tcagcgtttg cgattgttcc
    35581 ggcgtcacgc ctgcggaata cgctttctcc agtgtgaaga tcgaaacttc gctaccagca
    35641 aaaatcttca tactggcaac ggcggcaatc aatgctccca cgatggcgat ataaggtaag
    35701 tttttgataa tccgcgacgt gcgctcttca aacaccgaaa gcccgctggc atcgatgtca
    35761 ttttcatcac gatgacgcag gtcgtgagtt atcgcgatcc ccagcagcat caccatgcca
    35821 ataaagattt cgatggattc agggttaaga tgtgggaaat agcgcacgac taccacacgg
    35881 gtcatcagaa caaccacggc ggcgaccaga ctttgcttcc agccaaactg gtagaaaatc
    35941 gccaccagcg ggaacagtgc aaaagcagaa accaccggcg agcttaattc cccgaggcta
    36001 cctaatacat ccaccggcag cgcggtcagc agctggttaa ctggcaacag gcaagtaagg
    36061 atcaacacgc cccagatagc gccaagacca aacgccatca ggctgtttat cgccagtacg
    36121 ccgaggatat cggtaggaag aaagagtaac catgcgttga gcagccaggt tttcagggta
    36181 aaagagatcc ccaccgaggc cacaaaacca atgctcagac caaaagcgat actccccgcc
    36241 tcgcgacggt tcatatagcc ttcaatcagt tgcggcagga tcgggtggat gccgtcatga
    36301 aaaacagccg ccgagcgatg cgccagaagc gatgtcatac ccgtcaggca cgccaccacg
    36361 ataatctgaa tatacagatc catagtcgtg ccttttattt cagatggtta atcaacatcg
    36421 gaatcgcgtg ttcgacgtgc tcaacggaaa ggccaaacgc cactttccct tcagcgatca
    36481 ttttggcgat atgctcgtct ttcgctttat gccgcgcccg caccggtatt acacgcgcca
    36541 atgtaataat ctagttgccc ggatttcacc ttcattgccg cttccatgtc gttgtgaatg
    36601 aaaacttcaa aacagccagg agccgtcgct tcaatggttt ttttaatctg ctcacgctgt
    36661 aagcctgcaa cgccaatctt tttcatgagt ctgtccttat tggaaaaatt gagagggatt
    36721 ttcacgtaac atcacatcca catcggcctg actgaatcct gactggcaca attgcggaat
    36781 aaaggtggtc aataaatagt catagccata accaccattg gcttttaaat gggagcggcg
    36841 cgtaatatcc atcgacagca tgacgcggtt cagcaaccca cggtcacgta gcgcgtggag
    36901 catcgcaatg cgcttttcgt ccgggtagta actgttctta ccaatggtgt cgaactgcac
    36961 gtacgcgccg agatcgatca tcttcaaaat gttgtcgagg ttgtctttca gatcgcagtg
    37021 accaacggtg acgcgcgaaa gatcaactcc gtgggcttgc aacagagcca gttgttccag
    37081 ccccatggtg ctgaacgacg tatgcgtgga gatcgggcgt ccggtctggt tatgcgccag
    37141 cgcagcggca ataaatacct tctcttccag cggcgtaatc tttccttcgc tggagccgat
    37201 ctccgcgatg atcccggctt tcagctccgt gccatcgata ccctgttcaa tttcatcgac
    37261 catctcctgc gccagttcct gcacgctgcg ggtcaccaca tgttccggga aaaacgcatc
    37321 ctggtaataa ccagtacagg ccaccacgtt gatcccggtt tcgcgcatta catcgagcat
    37381 aaattgcgca ttgcgcccca tgtaacggtt ggtcatctca atcacattac gtacaccccg
    37441 ggtcatcagg tcgttcatct cctggcaaat gaacgcatac tgatcaaggc ggcagtccac
    37501 gttgttttta aagccggaga gatcaatatg cagatgctca tgggccaggg tgtaacccgt
    37561 cggatcaaaa ctcatagggt gtctcctgca aaccggaggg aggaaagaaa agagcgacca
    37621 ttttgtggcg gtggcgcacg gaaaaattca cacacggtag ctcccacatc agaaagcgtg
    37681 gtgcgcacac cgagctgcgt ggcgaccagc ccttgctgat aaaccagcac tggcaccact
    37741 tcacgggtat ggtggctgtg accaatggtc ggatcgttac cgtgatccgc catcacgacc
    37801 aggcaatcat ctggctgcat cgcctcaaca agccgggcaa ggttacggtc aacgacctgc
    37861 aaacgttcgg cataacgtgc gacgtcttct gcatgaccag cgaggtcggt ttcctggata
    37921 ttgatgcaaa taaacgccgt cggatgggtg ttaaattcgt cgagggtgat atccataatc
    37981 cgctggctat ccaccaggtt ttgccagctc acaccataag gattgctgac gatatctgcc
    38041 accttaccca ccagcacggt tggcacgccc gcttcataca gtttttgcgg cacctgcact
    38101 ttttcatcaa caccgtagcc catatgcacg acctggaagc cgttgtcata agcgccagaa
    38161 cgcggcgcat taataccaat aaagcgccct tctttgcttt ctgcggcatc gagaatacgc
    38221 tgactgttgg ttaacaggcc gccaaatgta atgacccgac cgacctgtac ctggtcacgc
    38281 acgatacgac caattttgat tgcgtcgtca aaagagatca cagagagatt ggcggtaatg
    38341 ttatagactt ggcctaaatc cgcctcgaga ttatcgccaa tcgcaaccgc ctgattgacc
    38401 cacagaaatt gcagatcatc gccacggcgc tccacctgcc agccagcgga aactaatgcc
    38461 tgctcaacac ggtcaatcac atcgcgaaaa ggcatccgca gcggcggtaa cgggcgcgtg
    38521 cctaaaattt cctgatgccc cataaaggta tcgccacctt catgttgcag ctctgccacg
    38581 ccccaggttg cggaatctga cggctgcata tcgcctggcg cataacccaa tgtgttgatt
    38641 agccccagct tctccaacgt tggtagctgc aaatgcggca actggctcag gatgtgacca
    38701 caggtattcg ccccggcatc ttgcggacgc accagcgtga catctttcat tgcccctacg
    38761 ccaaagctat caatcactaa caccacaaat cgcgccattt atgccttcag agggttgccg
    38821 aggctgtcat aacgaccaac tatttctggt tcgccgcgct gaataccgga caccagcacc
    38881 acatcgctac gggtgacaaa aatctgcgtg cgaaaacaga gcaccactgc actgctcacc
    38941 ggatactcgc ctgccagcgg cagggtatag tcgatactgc tgtcatccac ggttttgaga
    39001 ttggtttcag taatcttttg attttctggc gtaaacacca gcgcatgttg cgcatgacca
    39061 cgacggtagt aaccgccgcc gtagcagtag ctgtcgccac ggaaatgatg ggagatttca
    39121 cttaaccaga gcatcgcgat acgttcaggc tgatcgccct gctggtttgc cggaatagta
    39181 ccggtcagcg catgaccggg ttcggcatga gtcgcaccgt attccgccag caatggcagc
    39241 gaagtgcagc tggtcgctga aggtacgttc agttgctcaa tcgcaatacc agatttcgcc
    39301 agttgatccc gtgcttgtac cagcgtgtga agattcggtg ttggcaaaac tttcccggca
    39361 gcctcatccc aaagcaggca agggaaatgg gttagtccgg ctaaatgcag ccccggcagt
    39421 ttcttaattt cagcaatcac ctcgtgcagc gaatgctgta caaaaccgct ctcctgcccc
    39481 ggataaagaa aatcatcgtc gctataaact ttgagcagca caggctggac ccgtccggcc
    39541 ttaaccgctg ccgcagaaac ttcccgtgct ttgtcgagag taaacacggt gatgatgtcg
    39601 gtgccctgtt caacggcgtc agcaacctga tgacaaggga tttgtaccag atgcccctga
    39661 tgcgccactg gcaaaccagc gcggcgcatg actcgcgcct ctttgtaatc caccgccaca
    39721 atgccgctgt agcctaatgc cagcaatttt tccgccagcc acggattacg accaaattgc
    39781 ttggtcatca gatacagttc aatgccgtaa agccgcgccg tctcaatgag ccgcttaccg
    39841 ttttccagta tctgatccac gtcgatcacc cagctgtccg gggcgatttt gccctgctgc
    39901 cacaaactta gtgcggcgga aatcagcgcc gggttctggc gtttcaatgc ttctacaaac
    39961 atcttgttgc ctcgtttctg ttccttgaat taaatattca tttttttaaa tatttaggtt
    40021 gtgcgacgtg gatttgtcgg gagcttcaaa cctcttcgtt agcagccatc cataatccat
    40081 aaagattggc cagaaaatag ccttcttcac acgggttaac ttccagagcg aattccttca
    40141 acaacacctg atgcaattgc acaacggctt gccagtggct ggattgcgcc agttccgcca
    40201 gcaactcgtt atccagcggc tctatttctt caccacggcg actgcgcatc agtgcactcg
    40261 ccatatgtgt catcgccatc gttccttgct cactgcgcac cggcagatgg cactcttttt
    40321 ccagtacgtt gacgacctgc atcatgcctt tgcagacgtc cttatcaata acgcctgcct
    40381 cgcaaagcag gttgagtctg gtttccatat cagatgcctt tcgtttaata actgggttcc
    40441 tgttccccac tcaccacccg ttgttgatgg gcaagcaggg cgtgtttatc tacaacggca
    40501 cgcagcaatt gttgcattgg gtaatcatcg actcgcgtca ggaccacggc ttcggtggcc
    40561 agtaaaaatc gcggatcgtc tgttagcggc gtcgcctcta atcccagcat ggtcagttcg
    40621 ttttccgcca ccacgttcca gatcaccgca tcgacatcgc ctttaacaat gcgttgtaaa
    40681 ctctcgtgat aagagagatc gactcgttcc acatcactat cgccaaaaaa gacatcggtc
    40741 atgattttct gatccgccga acgactatcg agacctactc gcttaacatt tccagactca
    40801 cctttgcggc atatcagttg atgttcacca acatacgtat gcgggccaag ctcgagggcg
    40861 atgcacaaat tgttttggct gagataactt tccgccgcca ggcgggaaac tactgccatg
    40921 tcatagacac cgttaagtaa acactccacg cgaatatccg cgccgcgcat atgcgcatag
    40981 taaaaaggaa tgccatcaaa ctgggctttc aatccgctcg ctaaaccttc gtacaagcgg
    41041 gtatagggca aaggcatcgc acataccacg ttgttgatat ccacatgact cagcaatgct
    41101 ttgttatcca tctcgacaag ataactgcca ttgcgcccac ggcgttcaat ccgtatcgct
    41161 ccgcttgatt ccagcgtttt caacgcggcc tgcgtcaggc caaccgatga acggcattca
    41221 gttgccagct catctatggt tttcagtcga ttaccgcatt tttcacccaa caaataacgg
    41281 gccagtgtcg tgatgacgac gccttctttt ttgataaacg ttcgacgcat aatatatttt
    41341 caataaaatg aatatttata tcttcattaa aatgaagata agtggcgacg acgagatgtg
    41401 gaaacggcga ggcgcttcac attttttcgg gttgttgcga aaagaggata aaaaaaccgg
    41461 gtttccccgg tttcagagtg atgacgaagt gatttattcg acaatgcgag caaagggtaa
    41521 ttcccactgg cgcttgcctg ctcccctcac cctaaccctc tccccaaagg ggcgagggga
    41581 ccgtccgagc gttttgatat tacggcttcg ccacaaaacc aatcgcttcg tacaccgctt
    41641 tcagcgtacg ggaagcgtgt acactggctt tttctgcgcc gtctttcatc acctgttgca
    41701 ggaaggcttc atcgttgcgg aaacggtgat agcgttcctg caattcagtc agcataccgg
    41761 aaacggcatc agccacttca cctttcagat gaccatacat cttgccttcg aactgttttt
    41821 ccagttctgg gatgctctgg cccgttaccg cagaaaggat atccagcagg ttggaaacgc
    41881 ccgctttgtt ctgcacatcg tagcgaacta ccggcggctc gtcggagtca gtgaccgcgc
    41941 gtttgatttt cttcactacc gatttcggat cttccagcag gccgataacg ttattgcggt
    42001 tatcgtcaga cttggacatc ttcttggtcg gctccagcag cgacattacg cgcgcgccag
    42061 atttcggaat aaacggctcc ggcaccttaa agatctcgcc atacagcgcg ttgaaacgct
    42121 gggcgatatc gcgactcagt tccaggtgct gtttctggtc ttcacccacc ggtaccagat
    42181 tagtttgata cagcaggatg tccgcagcca tcagcaccgg atagtcaaac agaccagcgt
    42241 tgatgttctc ggcataacgc gcagatttat ctttaaactg cgtcatgcga ctcagttcgc
    42301 cgaagtaggt atagcagttc agtgcccagc ctaactgcgc atgttctggt acgtgggact
    42361 gaacaaaaat ggtgcttttc tcaggatcga taccacaagc cagatacaag gccagcgtat
    42421 ccaacgtcgc tttacgcagc ttctgtgcat cctggcgcac ggtgatcgcg tgttggtcaa
    42481 cgatacagta aatgcaatgg tagtcatcct gcatgtttac ccactgacgc agcgcaccca
    42541 tgtagttacc aatggtcaat tcacctgagg gctgtgcgcc actaaaaacg atgggcttag
    42601 tcatttttcg attcctgatt ttcgctatgc ggaagcccta atgcgggcag aaggtcattt
    42661 atagactgat aaattacatc aggctggctg agatcgatag cctcgccgta gttatatccg
    42721 taggttaagc caactgatgg gcaacctgcc gcttttgccg cctgaatatc attgcgtgag
    42781 tcgccgacaa acagcatctg ttgtggggca attcccatcc gctcagccac cagtaacagc
    42841 gggtccggat gcggtttttt gttttgcaca tcatcgccgc cgataaccac gctaaagtat
    42901 ttggcaatat ctaaggcttc gagcagcggc gcgacgaacg gcgtcggttt gttggtgacc
    42961 aggcctagcg gcaggccttt agcctgcaac gcgcccaacg tatcggcaac gtgcgggaac
    43021 aaaaacgtcc cctcttcggc aacctcgcca tagtagcgat cgaacagttt acgcagaata
    43081 cgtacctgtt cttctgccgg aatgtcgtca tcaacgggcg gtttccccat tgttttacgc
    43141 agagtcgcac gttcctgacg cgcccaggtc aatgcgcgct ccatcagaac atctgcgccg
    43201 ttaccaatcc aggtaataac gcgttcttca cctgcgacgg gcaactccag cgcatacagc
    43261 gccatatcta ccgcagcagc aagaccagga gcactgtcga ccagcgtacc atcaagatca
    43321 aaagcgacgc cgcgaatatc ttcaaactta ttcatgactt acctttgcca gttcactgcg
    43381 catttcatca atgacttttt tatagtctgg ctggtcgaag attgccgaac cggcgacgaa
    43441 catatccgcg cccgccgcag cgatttcgcc aatgttgttc accttcacgc caccgtccac
    43501 ttccagacga atgtcaaagc cagactcgtc gatacggcga cgtacttcgc gcagtttatc
    43561 cagtgtttga ggaatgaaag actgaccgcc gaaaccaggg ttgacggaca tcagcaggat
    43621 cacatccagc ttatccatca cgtaatccag atagctcaga ggtgtcgccg ggttaaatac
    43681 cagacccgct ttacagccat tttctttaat cagttgcagc gtgcggtcaa catgctcgga
    43741 ggcttctgga tgaaaggtaa tgatgctggc accagcggca gcgaaatcag gcacaatgcg
    43801 atcgacgggt ttcaccatca gatgtacgtc gataggggcg gtaatgccgt agttacgcaa
    43861 ggatttcagc accattggcc caatcgtcag attgggaaca tagtggttat ccatgacgtc
    43921 aaaatgcacg acatcagcgc cagctgccag ggcttttgcg gtatcttcac ccaggcgggc
    43981 aaaatcagcc gacagaattg agggggcaat caaatactgt ttcatccgct tctccttgag
    44041 aattattttt tcgcgggtga aacgactcct ggtttgtaca aagccagcag ttcgtccacc
    44101 tttttacgtg tgccgccgtt gctgcttata ctgcgtcgaa ctttgacgac atgcaatttt
    44161 gcgcgctgat accactcacg cgttaacatc gtatcgtgat tggagatcag cactggaata
    44221 tggcgctcaa ccagaccttc ggcgatctcc gccagatgcg cttgttgttc aagcgtaaaa
    44281 ctgtttgtgt gatacgccgt aaagttggcg gtcgcagaca gcggtgcata aggcggatcg
    44341 caatagacga cggatgcatc atctgcgcgc tccatgctat cggcgtaaga ctcacaatag
    44401 aaaaaggcat tctgcgcttt ttcagcgaag tgatacaact ctgcttccgg gaaatagggt
    44461 tttttgtagc ggccaaacgg cacgttaaac tcaccgcgca gattgtaacg acacaggccg
    44521 ttgtaaccgt agcggttcaa atataaaaac agtaccgccc gacggaacgg atcctggctt
    44581 ttgttgaact cttcgcggaa ctgatagtaa acctcggcgc aatttgtttc gggaacaaac
    44641 agctcgcgtg cggcctgtac gtactcatca gtacgcatct tcacaatgtt atagagactg
    44701 atcaggtcgc tattgatatc ggcaaggata taacgagaaa agtcggtgtt gagaaacacc
    44761 gacccggcac ctacaaaagg ctcaaccaga cattcgccct tgggcaaatg ccgtttaata
    44821 tcatcaagca ggggatactt gccccctgcc cacttcaaaa aagcgcgatt tttcttcatg
    44881 ccgactaact aattacacct tctccggctg tggagaaagc tccgacagca tccagcgctt
    44941 caggcagctc ccgcaacgat aaaccattgc gggagataac cttgattact tcagatcggc
    45001 ctgtacctga cgcagcggtt tcgcccacgg gtttttggcc tgaacatctg ctggcaatgt
    45061 agataccgct tttttcgcct cttctttcga agcgtacacg ccagaaacca ggacatacca
    45121 cggctgacca ttacgcgtcg tttcatagac aacgtagttt ttcagattct ctttcttcgc
    45181 ccaaccgttc aggttgtcgt agttagagga actgctcagc tgcagagtgt aatggctgga
    45241 cggtgccgat ttcaacgaac caacattacc tgcggtcttc cctccagcgg ctggtgttgc
    45301 cgtggtttgc gccggggatg ccgtctgtac tggagccgta gtcgccgtct cttttggtgc
    45361 tggcgtagaa gtcgcagccg gtgccttcgt gctcgccaca ggggcagctg gttcagtacg
    45421 cttcggcgtc tgtgctaccg gcttcggctc cgttttcacg gttgcttgcg gttttttcgg
    45481 ttcaatcacc gcctgctgac gaactgggcg cgtagtggac ggacgttcag cggtttgcgt
    45541 tttcgccgtg tcacgcgatg cattgccatt gcgaacaggc gcaaccgttg cgggttcagt
    45601 cggcaacgtg gaattgaccg ccacattgtt cagctgttgc tgattttgtg gctgggtcag
    45661 cgcattgttc aggtcaccct gcacttcaac acgttgttga ccatccgttg ccaccggggt
    45721 ttgcccttga gtcggcgtag aagagatcgg tggcagagaa acatcctgct gagtattttc
    45781 cgcagacgtg gttcccggcg ctggctgcac gccattcgcc tgatcggtcg cattgccagc
    45841 aagatcaata ctcttctcgc cagacgcggt ttgatcgctg gaagtggtcg agggggcttt
    45901 tagcgcagaa ccgataccga tgatcaacag cagtagaacc agaatgccga cgcccatcat
    45961 catatactga cgagaagcgg gtttactggc tgcttttttg cgcttacgcg gacgacgctc
    46021 tacgcgctct tcatccacgg tttcatcttc ggattcgtca atttcttctt cgatttccgg
    46081 ttcctcattg cgctcttttt gcgcacgagt cggacggcga tcgtcagtgt catcaagttc
    46141 aatatcatca aaattgatct gcggttcgcc acgttcagta cgctcagaac gttcagaaga
    46201 ttgacgagaa cgaccagtac gacgatcgct gggatcgggt ttcagctcgt cttctggttt
    46261 gaattcatcc atttaacacc ccactaaaag gttaatgctt accacgttgc aattaacctg
    46321 aagctaatag accgcttgat aagcggcctg acctttcttg ttgttacgct gattgacaat
    46381 cggcaatggc gttaagaaca agctcgtgcg aaacgccgct gcgaacttca ctcttaccaa
    46441 ttgccaacgg aagaattaag cgcatctctc ccgcaaggac tttcttgtca cgcagcatat
    46501 gcggtaaata cgcctgcgcg gacatttcgc gcggcccatt gaccggtaac ccagcccgcg
    46561 tgagcagggt tataatacgc tgcgtttcgg cagaactaaa ctgcccgaga cgttccgacg
    46621 tccgcgccgc catcaccata cccgcagcga ccgcttcacc atgtaaccaa ttgccatacc
    46681 ccatttcagc ttcaatggca tgaccaaagg tgtgtcccag attcagtaaa gcacgtaacc
    46741 cggtttcgcg ctcgtcggca gcgacaactt ctgccttcag ttcacaacaa cggcgaatac
    46801 agtacgccat tgccggaccg tccagacgca acaacgcatc cagattctct tccagccagt
    46861 taaaaaacgc accgtcaaga ataatgccgt atttgatgac ttctgccagc cccgacgcta
    46921 actcacgcgg gggaagcgtt ttcagacagt cgagatccac caccactgaa gcaggctggt
    46981 agaacgcgcc aatcatgttt ttaccgaggg gatggttgac cgcagttttg ccgccaacgg
    47041 aggaatcgac ctgcgacagt aacgtcgtcg ggacttgaat gaaacgaaca ccgcgctgat
    47101 aactcgccgc cgcgaagccg gtcagatcgc ccactacgcc gccgccaagc gccaccagcg
    47161 tagtatcgcg accatgcggc ttttgtaaca acgccgtaaa gacggtatcg agtacagcta
    47221 ggcttttata ctgctcgccg tcagggagga taacgctatc gacgttaaca cccgcctgtt
    47281 caagtacgcc gcggacctta tcgagataca gaggagccag ggtttcgctg gtgaccaaca
    47341 tgacctgctc gcccgatttc agcggtaaga atgaagctgg ttcattaaac aaaccagatg
    47401 cgatggtaat tgggtaacta cgttccccga gagtaacgac aatcctctcc ataacgcgac
    47461 atccacctta attactgtac ccgcagacga gtgtatataa agccagaatt agttgctttc
    47521 cagcatgtga ataatctggt ttgcaaccac tttagcgctt tgatcatcag tacgaatggt
    47581 cacgtcggca atctcttcat acagcggatt gcgttcattg gccaacgctt ccagaacttc
    47641 acgcggcggt gtttcaacgt gcagcaacgg gcgtttttta tcacgctgcg tgcgtgcaag
    47701 ttgcttttcg atggtcgttt caagataaac gacaacgcca cgagcggaaa gacggttacg
    47761 cgtttcacgg gatttcacag agccgccgcc agtagccagc acaataccct gtttctcggt
    47821 caactcattg atgacctttt cttcgcgatc gcggaagcct tcttcgcctt ctaaatcgaa
    47881 aacccagccc acatcagctc cggttcgttt ctcaatctct tgatcggaat cgtaaaattc
    47941 catattgagt ttttgagcta actggcgccc aatagtgctt tttccggcac ccataggccc
    48001 aaccagaaag atattgcgtt tctctgccat tttttcggta ctactaagac tattcgttaa
    48061 tgataaaccc gcttcgctca gagagcgccg caggacatga actgaaacct cataagatat
    48121 tgcgagagtc agactgaaaa ttatctcaat actccagcgg gtttggcaac tgaataaatc
    48181 acccatccct ctgcatatct ggtcgctgca agcgcactgc cttgctacca ccgctctggc
    48241 gataaatcac cgggtaagat tagcgtaaaa aagacagcaa aatgccgcct gaatgataaa
    48301 tcatcatcat ggaacgcagg cagcgaagag caccaactct caaatcggta ctccttgtat
    48361 gctaaatacc tgcgcgcgtc aaatagatga aacacgttca gcataaaaac attaccgctt
    48421 ttacggctgt ttactcactg gaaaccagtc gtggcgtgat aaacaccact aactcgcgtc
    48481 gttcatcttc ttttccgtca tgacgaaata attgcccgaa ccagggaatg tcgccaagca
    48541 acggtacgct atcctgaccc gatttatttt tacgggtaaa aatgccgccc agcgccaacg
    48601 tttctccgct ttttacctcg acctgcgttt cgatctcctg cttatcaatc gccagcactt
    48661 cgccatcggc ctgctgtagc acctgccccg gaacgttctg gctgatgtgt aatttcagcc
    48721 ggatgcgacc tttttgtaac accgtgggcg tgacctccat ccccaggacg gcctctttaa
    48781 attccaccga cgtcgcgcca ctttccccgc tggaaacctg atatggaatt tcgctcccct
    48841 gtttaatgct ggcaggctga agatgtgagg ccagcagacg cggactggcg ataatatcca
    48901 gctgctgttt ttgttcgagc gcggaaagct caagatccag caagcgtccg ttgatgcgcc
    48961 caatgttaaa accgacatgc gttgtcgccg tcgctacgga gaggtcgcta ccaagcgtgg
    49021 tgacttgccc aacgccacca gcgtgttgcg catcggccag cgtccatttc acgcctaact
    49081 cacgcaaact tttttcatta atggtgacaa tatgcgccga cagctcaacc tgcccgaccg
    49141 gcagatccat ttgcgctacc cactgttcaa gcgcgcttaa cgccgttttg ttatctcgta
    49201 gcaaaaggcg attggtgcgt ttatcgacgg tcatactccc tttggcactc agtagcttct
    49261 cccccgcttt cgccagttct cccgcgtcgg cgtattgcag ggttatactg cgattttcca
    49321 gcggcagatt tgcctgcgcc cgcgcctgct ccgcctcctg gcgggcgata ttgtcattct
    49381 gccaggcaat ggaatgcact gagagaatgt tgccttcctg ccgcgttatc agtccggcgc
    49441 ttttcactac agtttgtagt gcctgcttcc agggaacatc tgttagatgt aacgacaccg
    49501 taccgctgac gtctggcgac acgaccaggt tcaacttctc ctgttcagcc agcgcctgca
    49561 acacctgagc taccggaacg tcatccacca tcagcgtcac tttttgcggc tttgccgcct
    49621 gtacgccgga tatcagcatc aacagtagtg cggctatcca ttgcttcatt tgtatctcct
    49681 tgccgttgcc acaaccattg tggcggttcg cagtttgtcc cggtacccag cgttagtacg
    49741 tctggcgtca gctgtaaaat tgtccagccg ttttccagca catcgttttg ctgcacccgt
    49801 cgccatttct tttgcccgtc ttttattaca ccgatgatgc gctcgcctcg ccctaccatc
    49861 ccctgatagc gccactggct aagttcgcta atccggcata gatcttccgg cgatttaaaa
    49921 gggtcacgca taccggttaa aaggcacaat gcaatacctg ccaacaacca gcgtttaacc
    49981 ctcatgcggc atctccagtt gtagcgtgaa caaaagatca tcaccttcca cgcttaacga
    50041 aaaacggctc acgctgacgt tgcgctctgc cagccgtgta aatgccgacg gcactgcttc
    50101 ccacagcgtt ttcaacgcca actcgcctcc ctgcgcggat ggatgccagg aaaccagttg
    50161 cgcgccggat aactgaaaat ccagtggcga aaagggcagc gttttttcct cgctgaaggg
    50221 agtggtgtct accaggcgat acagtgcggc ccactgctgg tgatgacttg cccgtagtcg
    50281 aattaatgcg tcgcgctctt catggtgtgt cgatgataga aaaatgagcg taactaacat
    50341 cagcaaccag aatgcccagc aaaactggcg gaggcggggt gatgtggcga accaccagtc
    50401 aaagaacatg ttcatcgcta accttccttg ttaactgata ctcaaattgc cagcgtccct
    50461 gcgcatcctg ctgcgtggct ccccgctgat tgagatgaaa agacgcatcc tggcggagtg
    50521 acgtttctag tgcgtttaac gcggtaatgc ttgttgtcag ccccttgatc tccagcgttc
    50581 cctgctgcca gcttatcgtt gtcagccagg cgtgctcggg taaaagcgcc gccagtgctt
    50641 ccagcgcaga ttgccagtcg cgggtaaatt gtcgctggcg ctgccgttgc gaacgctgtt
    50701 cgcgtaactg ctgttgctcc agcaaacgtg gcttcgttat ctgtaagctg cgggcgagtt
    50761 gttgttccgc ctgaagcaaa actgcgtcta tgcgcgcttc ggcgctgcct gtcagacgca
    50821 gtattagcgt tatcccgacg gccagcagca gaggcgcaac gaacatcagc aaccagaaac
    50881 gcagaaaagc ggtccggcgt tgctgtcgcc agggcaaaaa attaattggc gggttcatca
    50941 gtacgccttc ccaagcgcca gccccagcgc gatggcaaag tctccaccag gcggcggtag
    51001 cggcggctga cgaacagaaa cggcctccca ggggtcaaat ccgccttcgc cacatatcgc
    51061 gacgctgtcg ggatcaacgg ataacgctgc cgccagctcc ttcgcgctag tcatccccac
    51121 cgccagtttg cgcccccagc tatagcgcgt cgcccacagc cactgttcgt tatcacgcca
    51181 ggccagacat tgctgatgag aaggtaaaaa aggcaggaat cgctgtaatg cactggcatc
    51241 cggggtgatc gcactaacat gaacacgcaa cctttctgcc agcgtaagca gcgttgccag
    51301 ctctttgctt tgcgcggcag tcacgttata agcggggctg agtgagtctt cgctgtaatc
    51361 gaagcgcagg gagtccggat ccatatccag ctcgcgggcc atcgtccctg acagccaggc
    51421 cgtttgctcc cgctcaccaa gggacatcga cgggcgcgga aatgaccgct gtaatgtgcg
    51481 actggcggga aacgccaaca taatgtgatg acgctgcggc agttcgcgac tccacggtaa
    51541 caacgtttta gccagctgct gcgcatcaac aatccgccca tctttgataa tgtcgtgcgc
    51601 cagcggcaac cgccaccagc gttgcaaaaa gcattctttt gcgccccgca cgatcgcaac
    51661 cgctgccact tcttgctgtt gtaaatgcaa accaatttgc cagatcttaa atgtcattgt
    51721 gatgatctcc ttatcacccg tcactctgac gggtatatca atgcgtctgg cttgccttta
    51781 tactaccgcg cgtttgttta taaactgccc aaatgaaact aaatgggaaa tttccagtga
    51841 agttcgtaaa gtattttttg atccttgcag tctgttgcat tctgctggga gcaggctcga
    51901 tttatggcct ataccgctac atcgagccac aactgccgga tgtggtgaca ttaaaagatg
    51961 ttcgcctgca aattccgatg cagatttaca gcgccgatgg cgagctgatt gctcaatacg
    52021 gtgagaaacg tcgtattccg gttacgttgg atcaaatccc accggagatg gtgaaagcct
    52081 ttatcgcgac ggaagacagc cgcttctacg agcatcacgg cgttgacccg gtggggatct
    52141 tccgtgcggc aagtgtggcg ctgttctccg gccacgcgtc acaaggggcg agtaccatta
    52201 cccagcagct ggcgagaaat ttcttcctca gtccagaacg cacgctgatg cgtaagatta
    52261 aggaagtctt cctcgcgatt cgcattgaac agttgctgac gaaagacgaa atcctcgagc
    52321 tttatctgaa caagatttac cttggttacc gcgcctatgg tgtcggtgct gcggcacaag
    52381 tctatttcgg taaaacggtc gaccaactga cgctgaacga aatggcggtg atagccgggt
    52441 tgccgaaagc gccttccacc tttaatccgc tctactcaat ggatcgtgcc gtcgcgcggc
    52501 gtaacgtcgt actgtcgcgg atgctggatg aagggtatat cacccagcaa cagttcgatc
    52561 agacacgcac tgaggcgatt aacgctaatt atcacgcgcc ggagattgct ttctctgcgc
    52621 cgtacctgag cgaaatggtg cgccaggaga tgtataaccg ttatggcgaa agtgcctatg
    52681 aagacggtta tcgcatttac accaccatca cccgcaaagt gcagcaggcc gcgcagcagg
    52741 cggtacgtaa taacgtgctg gactacgaca tgcgccacgg ctatcgcggc ccggcaaatg
    52801 tgctgtggaa agtgggcgag tcggcgtggg ataacaacaa gattaccgat acgctgaagg
    52861 cgctgccgac ctatggtccg ctgctgcctg ccgcagtcac cagcgccaat cctcagggag
    52921 cgacggcgat gctggcggac gggtcgaccg tcgcattgag tatggaaggc gttcgctggg
    52981 cgcgtcctta ccgttcggat actcagcagg gaccgacgcc gcgtaaagtg accgatgttc
    53041 tgcaaacggg tcagcaaatc tgggttcgtc aggttggcga tgcatggtgg ctggcacaag
    53101 tgccggaagt gaactcggcg ctggtgtcga tcaatccgca aaatggtgcc gttatggcgc
    53161 tggtcggtgg ctttgatttc aatcagagca agtttaaccg cgccacccag gcactgcgtc
    53221 aggtgggttc caacatcaaa ccgttcctct acaccgcggc gatggataaa ggtctgacgc
    53281 tggcaagtat gttgaacgat gtgccaattt ctcgctggga tgcaggtgcc ggttctgact
    53341 ggcagccgaa gaactcacca ccgcagtatg ctggtccaat tcgcttacgt caggggctgg
    53401 gtcagtcgaa aaacgtggtg atggtacgtg caatgcgggc gatgggcgtc gactacgctg
    53461 cagaatatct gcaacgcttc ggcttcccgg cgcaaaacat tgtccacacc gaatcgctgg
    53521 cgctgggttc agcgtccttc accccaatgc aggtggcgcg cggctacgcg gtcatggcga
    53581 acggcggctt cctagtggac ccgtggttta tcagcaaaat tgaaaacgat cagggcggcg
    53641 tgattttcga agcgaaaccg aaagtagcct gcccggaatg cgatattcca gtgatttacg
    53701 gtgatacgca gaaatcgaac gtgctggaaa ataacgatgt tgaagatgtc gctatctccc
    53761 gcgagcagca gaatgtttct gtaccaatgc cgcagctgga gcaggcaaat caggcgttag
    53821 tggcgaagac tggcgcgcag gagtacgcac cgcacgtcat caacactccg ctggcattcc
    53881 tgattaagag tgctttgaac accaatatct ttggtgaacc aggctggcag ggtaccggct
    53941 ggcgtgcagg tcgtgatttg cagcgtcgcg atatcggcgg gaaaaccggt accactaaca
    54001 gttcgaaaga tgcgtggttc tcgggttacg gtccgggcgt tgtgacctcg gtctggattg
    54061 gctttgatga tcaccgtcgt aatctcggtc atacaacggc ttccggagcg attaaagatc
    54121 agatctcagg ttacgaaggc ggtgccaaga gtgcccagcc tgcatgggac gcttatatga
    54181 aagccgttct tgaaggtgtg ccggagcagc cgctgacgcc gcctccgggt attgtgacgg
    54241 tgaatatcga tcgcagcacc gggcagttag ctaatggtgg caacagccgc gaagagtatt
    54301 tcatcgaagg tacgcagccg acacaacagg cggtgcacga ggtgggaacg accattatcg
    54361 ataatggtga ggcacaggaa ttgttctgat taaaaaggcg cttcggcgcc ttttcagttg
    54421 gctgacaaag tgcatttgtt tatgccggat gcgggtgaac gcgttatccg gctaacaaaa
    54481 tcgtgaaaaa tcaatatatt gcaggaaccc cataagcctg ataagcgttg tgcatcaggc
    54541 aaacttcacg catttacact cgcccctgcc ctttcaacca ttcgcgcacg aggaacagcg
    54601 cgctgacatt acgcgcttcg ttgaagtcag ggtcttccag caaatccatc atatgcgcca
    54661 gcggccagcg cacctgtggt agcggctctg gctcatcgcc ttccagcgat tccgggtaga
    54721 gatcttgcgc gaccacgata ttcattttac tggaaaagta agacggtgcc atgctgagct
    54781 tcttcaaaaa agtcagatcg ttcgcaccaa atccaacctc ttcttttagc tcgcggttag
    54841 cggcttcgta gacgctttca cccggatcaa ttaatccttt cgaaaaacct aattcgtagg
    54901 attcagttcc cactgcgtat tcgcggatca ggatcaggtg atcgtccaca atcggcacaa
    54961 tcatcactgc ttcccggttg gttggacgca ttcgttcata aacacgccgc acgccattgc
    55021 tgaactccag atccacactc tcgacggtaa acagtcggga acgggctaca gtttcaacat
    55081 tcagaatggt gggtttttgt aatgatttgt tcatcgtggg atctatgctg tgaaatcagc
    55141 ggttattgtg cgatatcgga cacgctttcg gcaatgtgaa ttgcatgtta tttacattta
    55201 tgtaacttaa taaataatcg tcctcaaatc aaattaaaag tcaataggtt gaaataactc
    55261 caggaatttg ctgatattcc gccttcagag ggtttgctat gatcagcggt tactgtgatg
    55321 tgcttaatga tgctcaagtt aaactccacg cttgccgata gccaaccgca gaatcatgta
    55381 ttgtgtccgg tgcgactgac cacgcctgac agactaagta agatggggaa agcatgagca
    55441 ccattgtgat ttttttagct gctttgctgg cctgctcact acttgcggga tggctgataa
    55501 aagtgcgatc cagacggcgt cagctgccct ggaccaacgc cttcgcggat gcgcaaacgc
    55561 gtaaactcac acctgaagaa cgtagcgccg ttgaaaatta tcttgagagc ctgacgcagg
    55621 tattacaggt gcctggccca acgggagcta gcgcggcacc gatctctctg gcgctgaatg
    55681 ccgaaagcaa caacgtcatg atgctgacac acgctatcac acgttacggc atctctaccg
    55741 acgatccgaa taaatggcgt tactacctcg attcggtaga agtccacctg ccccccttct
    55801 gggaacagta catcaacgat gagaataccg ttgaactgat tcataccgat tcgctgccgt
    55861 tggttatttc actcaacggt catacgctgc aggagtacat gcaggaaacg cgcggctatg
    55921 ccttgcaacc tgttccgtcc acgcaggcgt cgattcgcgg ggaagagagt gagcaaatcg
    55981 agctactcaa tattcgcaaa gaaacgcatg aagaatatgc gctgagtcgt ccgcgcgggc
    56041 tgcgtgaagc gttgctgatc gtcgcctcct tcctgatgtt ctttttctgc ctgattaccc
    56101 cggatgtatt tgttccgtgg ctggcaggcg gcgcattact gctgctgggc gcaggtctgt
    56161 gggggctatt cgcgcccccg gcaaaatcct ccctgcgaga aattcattgt ctgcgcggta
    56221 caccccgtcg ttggggattg tttggcgaaa acgatcagga acagatcaac aatatttcac
    56281 tcggtattat cgacctggtc tatcccgcac actggcagcc atacattgct caggatctcg
    56341 gtcaacaaac cgatattgat atctatctag accgccacgt agtgcgtcag gggcgatatc
    56401 tttcgctgca tgatgaagta aaaaactttc cgttacagca ctggctgcgc agtacgatta
    56461 tcgctgcggg ctcgctgctg gtgctgttta tgctgttatt ctggatcccg ctggatatgc
    56521 cgctgaaatt cactctctca tggatgaaag gcgcgcagac cattgaagcc accagcgtaa
    56581 aacaactggc tgacgctggc gtgcgggtag gcgatacatt gcgtattagc ggtacgggaa
    56641 tgtgtaatat tcgcacttcc ggaacctgga gcgcgaaaac caattcacct tttttaccat
    56701 ttgactgctc gcagatcatc tggaatgacg cccgctcatt accattacca gaatctgaac
    56761 tggtcaacaa agcgacggca ttgactgaag cagttaatcg ccagctgcac cctaaaccgg
    56821 aagatgaatc tcgcgtcagt gcctcattac gttcagcgat tcaaaaatcc ggcatggtat
    56881 tgcttgatga ttttggcgac attgtactga agacagcgga tttatgttct gccaaagatg
    56941 actgtgtgcg actgaaaaat gcgctggtca atctcggcaa cagtaaagac tgggacgcgc
    57001 tggtaaaacg cgccaacgct gggaagctcg atggcgtgaa tgtgttatta cgcccggtga
    57061 gtgcggaatc gctggataac ctggtggcaa cctccaccgc gccgtttatc acgcatgaaa
    57121 cggcgcgagc ggcacaatca ctaaacagtc cggcccccgg cggattcctg attgtcagcg
    57181 acgaaggcag cgattttgtt gatcagccct ggccttcggc atcactttac gactacccgc
    57241 cgcaagaaca gtggaacgct ttccagaaac tggcacaaat gctgatgcat acgccgttta
    57301 acgccgaagg tatcgtcaca aaaatcttca ctgacgccaa tggtacgcag catattggcc
    57361 ttcatccgat cccggatcgt tccggcctgt ggcgctatct cagcaccaca ttgctgctac
    57421 tgacgatgct gggtagcgcc atttacaatg gcgtacaggc ctggcgtcgt taccagcgtc
    57481 atcgcactcg catgatggag attcaggcct attatgaaag ctgcctgaac ccgcaactga
    57541 tcaccccttc agaaagcctt atcgaataac acgtttgcgc ggcaggttat gctaccctgt
    57601 cgcgcaaatt gcttcactct ggagatttcc ctcatgcata tcaacattgc ctggcaggac
    57661 gtagataccg ttctgctgga tatggacggc acgttgctcg acctcgcctt cgataactat
    57721 ttctggcaaa agctggtgcc tgaaacatgg ggcgcgaaaa acggggttac gccacaggaa
    57781 gcgatggaat atatgcgcca gcaatatcac gacgtacagc atacgctaaa ctggtactgt
    57841 cttgattact ggagtgagca actgggtctg gatatctgtg cgatgaccac cgagatggga
    57901 ccgcgcgccg tactgcgtga agataccatt ccgtttcttg aggcactgaa agccagcggt
    57961 aagcagcgaa ttttgctcac caatgctcat ccgcacaacc tggcggtaaa acttgagcat
    58021 acgggtctgg acgcacacct tgatttatta ctttccaccc acacatttgg ttatccgaaa
    58081 gaggatcagc ggttatggca tgcggtggcc gaagctacgg gtctgaaagc tgaaagaacg
    58141 ctgtttattg atgacagcga agcgattctc gatgctgccg cgcaatttgg tattcgttac
    58201 tgcctcggcg tgactaatcc tgattccggg attgccgaga aacagtatca acgccatccg
    58261 tcactgaatg actaccgccg cctgatcccc tcgctaatgt gaaggagacg ccatgaaaga
    58321 gaaacctgct gttgaggttc gactggataa atggctatgg gctgcccgtt tttataaaac
    58381 ccgcgcgctg gcccgtgaaa tgattaaagg cggtaaggtg cattacaacg ggcagcgcag
    58441 caagccgagc aaaatcgtcg agctgaatgc cacgctcact ctgcgccagg gaaatgacga
    58501 acgcacggtg attgtaaagg cgattactga acagcgtcgc cccgccagcg aggcagcctt
    58561 gctgtatgaa gagactgcgg aaagtgtaga gaaacgcgaa aaaatggcga tggcacgtaa
    58621 acttaatgcc ttaaccatgc cgcacccgga ccgacgcccg gacaaaaaag agcgccgcga
    58681 cctgttacga tttaaacacg gcgacagtga ataactgtca cctgcaagag agatgattat
    58741 gccgcaacat gaccaattac atcgctatct gtttgaaaat tttgccgttc gcggcgaact
    58801 ggtaaccgtt tcggaaaccc tgcaacagat ccttgagaac cacgattatc cgcagcccgt
    58861 taaaaacgtg ctggcagaac tgctggttgc gaccagcctg ttaaccgcta cgctgaagtt
    58921 tgatggtgat atcaccgtac agctgcaggg cgacggtccg atgaatctgg cggttattaa
    58981 cggtaacaat aaccagcaaa tgcgcggtgt ggcgcgcgtg cagggcgaaa ttccagaaaa
    59041 tgccgacctg aaaacgctgg tcggcaatgg ttacgtggtg atcaccatta ccccgagcga
    59101 aggcgaacgc tatcagggcg tagttggtct ggaaggtgat accctggcgg cctgcctaga
    59161 agattacttt atgcgttctg aacagctgcc gacgcgcctg tttattcgca ccggcgacgt
    59221 agacggcaaa ccggctgcag gcggtatgtt gttgcaggta atgcctgcgc aaaataccca
    59281 gcaggacgac tttgaccacc tggcgacgct aaccgaaacc atcaaaaccg aagaactgct
    59341 gaccttaccg gcaaacgaag tgttgtggcg tttgtatcac gaagaagagg tgacggttta
    59401 cgatccgcag gatgtggagt tcaaatgcac ctgctcgcgt gaacgttgcg ccgatgcgct
    59461 gaaaacgctg cctgatgaag aagttgatag catcctggcg gaagatggcg aaattgacat
    59521 gcattgtgat tactgcggta accactatct gttcaatgcg atggatattg ctgaaatccg
    59581 caacaacgcg tctccggcag atccgcaagt tcattaatga gtatgtccgg cagagagggc
    59641 tctgccggat tttacgactt aatggcctga tgtgctgcgc ttaccagcgc ctatgaaata
    59701 aatcgcaaat tattttggtc agatacagcg tcgtatccga taaaagcgtt gtcacttact
    59761 taagtttatt tccagcgttt caccatctac cgtcagatcg cgccacgctt tcggcctcgt
    59821 ccccagtttt ccatccttcg cagctgtgag taatttttct ttcgttattt cgttcggaag
    59881 taaactcata tcaagcgcgt cccattcttt tctgtctgag tcaaagccat agacaatgac
    59941 tctgtcatca ttaaaagcaa acaggatccg ctccgcctga ccatcactat tcagatcttg
    60001 ctcgaccagt acgcaggcgt ctttttcaat acaggtcatc acgttatagc gatcctggat
    60061 taaggccgac cagaatgtcg catccggttt aacagaacca ggggcaatta acacattttc
    60121 ggctaatact ttttccgata cctgttcctg aagatgttgc tctccatcta acgccatcag
    60181 cagatcccgc gcgcgtttcg ggtctttcat aaacccggca tcgcttttca gcgactcaag
    60241 cgccgcacgt ccatagcgac cgctctgttc gagcatgtag atagttacct ggtctggcgt
    60301 gtttttgccg ctctgataac gcgccatatg actgttcacg ctaatacgca tactgtccag
    60361 caccggcgaa ttaagaagca ccagtatcac caacaccaat aacgaaaccg caaggttcac
    60421 tttgccctga agaacaaggg gattttgccc attacgccag acgatactga caaaataccc
    60481 cagcgaccac accagtaaca ccagctccgc cagcgcgccc tgcaagcggt cgacagtcca
    60541 gccgtactga gcgacccgca gccataaccc ccaggtggcg acgaacacat acagcggagc
    60601 aaccagcaaa gcggttttaa tcaggcaacg caacggcccc gtccagggaa gtgacgcttt
    60661 ttgcggatcg cggacaatag ccattaagat caattgcaaa aaggccagcg ttaacagcaa
    60721 cccggcggcg gagatgtggc gagaaatcgc gctcaggccc gtaaacggca gggtgatgat
    60781 aaacatcagg gttagcaatg atactaacgg cagcaacccc gtggcgatta gcgtgaacaa
    60841 cttttgaata gagtcgatta aacgtgactg tgtacgcgcg aggatcaccg ccagtgcggt
    60901 aaccagacca aatgtaagat acataaacca gtcggttgca aaaaagagcg tattgaaaaa
    60961 cgtgatgcca acaagtttaa acaactcacc ccacagtaaa agcaccagcc acgtcaggcc
    61021 attggagaga aaaatcacca gtaatataag tacgttatgc catactgact ggtaaaagta
    61081 gctatagcgg ctactgccat tgcggatacg aaggctttgt tgtatccacg gtagcaacaa
    61141 cattgccatc aacagcagat agcagccaaa atcccaaatc gccttttcag ctctccaggg
    61201 gttcatgcca tcagtttgcc acttcagcca accgctcatc cctgtcgtgg caataaacac
    61261 cagcgccagc catccccaaa ggcgcttctg tttaaaagag atcacggaga agagtaaaac
    61321 ggatgaaaac gcgacggtcg caggcacgcc ataaacaatc caactgtgat tttttccggc
    61381 aagccaggcg ataagcaggt agcaaaccag cccctgaagt aatccggtag caatcatccc
    61441 ccagcgtgtt gccggtgaaa gttcaacgtt atccatgttg gttatccaga atcaaaaggt
    61501 gggttaatta tcgcatccgg gcagtagtat tttgcttttt tcagaaaata atcaaaaaaa
    61561 gttagcgtgg tgaatcgata ctttaccggt tgaatttgca tcaatttcat tcaggaatgc
    61621 gattccactc acaatattcc cgccatataa accaagattt aaccttttga gaacattttc
    61681 cacacctaaa atgctatttc tgcgataata gcaaccgttt cgtgacagga atcacggagt
    61741 tttttgtcaa atatgaattt ctccagatac gtaaatctat gagccttgtc gcggttaaca
    61801 cccccaaaaa gactttacta ttcaggcaat acatattggc taaggagcag tgaaatgcgc
    61861 gttaacaatg gtttgacccc gcaagaactc gaggcttatg gtatcagtga cgtacatgat
    61921 atcgtttaca acccaagcta cgacctgctg tatcaggaag agctcgatcc gagcctgaca
    61981 ggttatgagc gcggggtgtt aactaatctg ggtgccgttg ccgtcgatac cgggatcttc
    62041 accggtcgtt caccaaaaga taagtatatc gtccgtgacg ataccactcg cgatactttc
    62101 tggtgggcag acaaaggcaa aggtaagaac gacaacaaac ctctctctcc ggaaacctgg
    62161 cagcatctga aaggcctggt gaccaggcag ctttccggca aacgtctgtt cgttgtcgac
    62221 gctttctgtg gtgcgaaccc ggatactcgt ctttccgtcc gtttcatcac cgaagtggcc
    62281 tggcaggcgc attttgtcaa aaacatgttt attcgcccga gcgatgaaga gctggcaggt
    62341 ttcaaaccag actttatcgt tatgaacggc gcgaagtgca ctaacccgca gtggaaagaa
    62401 cagggtctga actccgaaaa cttcgtggcg tttaacctga ccgagcgcat gcagctgatt
    62461 ggcggcacct ggtacggcgg cgaaatgaag aaagggatgt tctcgatgat gaactacctg
    62521 ctgccgctga aaggtatcgc ttctatgcac tgctccgcca acgttggcga gaaaggcgat
    62581 gttgcggtgt tcttcggcct ttccggcacc ggtaaaacca ccctttccac cgacccgaaa
    62641 cgccgcctga ttggcgatga cgaacacggc tgggacgatg acggcgtgtt taatttcgaa
    62701 ggcggctgct acgcaaaaac tatcaagctg tcgaaagaag cggaacctga aatctacaac
    62761 gctatccgtc gtgatgcgct gctggaaaac gtcaccgtgc gtgaagatgg cactatcgac
    62821 tttgatgatg gttcaaaaac cgagaacacc cgcgtttctt atccaatcta tcacatcgat
    62881 aacatcgtta agccggtttc caaagcgggc cacgcgacta aggttatctt cctgactgct
    62941 gatgctttcg gcgtgttgcc gccggtttct cgcctgactg ccgatcaaac ccagtatcac
    63001 ttcctctctg gcttcaccgc caaactggcc ggtactgagc gtggcatcac cgaaccgacg
    63061 ccaaccttct ccgcttgctt cggcgcggcg ttcctgtcgc tgcacccgac tcagtacgca
    63121 gaagtgctgg tgaaacgtat gcaggcggcg ggcgcgcagg cttatctggt taacactggc
    63181 tggaacggca ctggcaaacg tatctcgatt aaagataccc gcgccattat cgacgccatc
    63241 ctcaacggtt cactggataa tgcagaaacc ttcactctgc cgatgtttaa cctggcgatc
    63301 ccaaccgaac tgccgggcgt agacacgaag attctcgatc cgcgtaacac ctacgcttct
    63361 ccggaacagt ggcaggaaaa agccgaaacc ctggcgaaac tgtttatcga caacttcgat
    63421 aaatacaccg acacccctgc gggtgccgcg ctggtagcgg ctggtccgaa actgtaatga
    63481 tttgaagctg gagaatatct atccagtatc ttatagaaag caaaacggga ggcaccttcg
    63541 cctcccgttt atttaccctt cttttgtcgt gccctgcgcc cgcgttaccg gcactggcag
    63601 ccaggcgcga atggaaagcc cgccccgctc gctggtgcca agctccagca tcccgttatg
    63661 gttatccacg atacgctgca caattgccag ccctaatccc gtgccgctaa tggtgcgcgc
    63721 actgtcgccg cggacaaacg gctggaacag gtgcttacgt tgttccggcg caattcccgg
    63781 accgtcatct tccacctgga accaggcgcg attcggctcc gttccgctgc tgactttgat
    63841 ccagccattg ccataacggg cggcgttgac caccatattc gccaccgcgc gtttgatcga
    63901 cagcgggtgc attttcactt caatgctgcc ggggtaaagc gcggtttcaa tttcccgctc
    63961 atagccactt tcggcagcaa tcacctcacc gagcactgca ttaagatccg ccatttccat
    64021 cggcatctcc tgcccggtgc gcaggtagtc gataaactgc tcaatgatgg cgttgcactc
    64081 ttcgatatct ttattgatcg attctgccag atagccatcc tgctcgctca tcatctcagt
    64141 cgccaggcga atacgcgtca gcggcgtgcg caagtcgtga cttacccccg ccatcagcag
    64201 cgtgcggtca tccgccagtt gcttaacacc agccgccata tggttaaagg cacgggtaac
    64261 ggaacgcacc tccgaagcgc catattcacg cagcggcggc ggaataatcc ctttaccaac
    64321 ctgcaaggct gcgtgttcga gatcgaccaa cggtcggttc tggatacgaa taaacagcca
    64381 cgccccgcct atcgccaata gcataatcgc cagcgtatag cggaacagcg gagagaaatc
    64441 gccctgatga atttcggtca gcggcacgcg tacccagata ttgggcgaca gccaggtttt
    64501 cagccagacg acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg ggccgcccag
    64561 ttgctgtgcc atctgatggc ttaagaattc atagtgttgc gcccaacgca gacctgcctc
    64621 ttcggcagcc tcgttggagt agagagagat ccccagctca cggtagatct cccgacggaa
    64681 agcgggaggc acaaccaact gcgtgccgtc ctccagttgc agtttatcgg tcatcaacat
    64741 acgcacttcg tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa tcgcgaagtt
    64801 cagcaccacc agataagtcg tcaccaggct ggcgaacagc aaggtgacga tgagcaataa
    64861 cgtacgggca aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt agagccgtcc
    64921 ggtacaaaga cgtagcccag gccccaaacg gtctgaatgt aacgcggatg cgctggatct
    64981 tcttctacca tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc cattgcggaa
    65041 tattcacgac cacgggcaag gttcatcagc ttatcgcggg agagcggctc acgcggatgg
    65101 ctgaccagtg ccttcagtac cgcaaactca ccgctggtga gcggcatcgg ctcatcttcg
    65161 cggaacattt cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat taccgcctct
    65221 tcctgtgacg gtgcgcctgg cagttcgttc gcctgacgac gcagcaccgc acgaatacgg
    65281 gccagcagtt cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc aatctccagg
    65341 cctacgatac ggtccacttc ttcccctttc gccgtcacca taatgatcgg catcgggttg
    65401 ctctgactac gaagacgtcg gcaaatcgac aagccatctt caccaggtaa cattaaatcc
    65461 agtaccataa gatggaaaga ttcacgagtc agcaggcgat ccatctgttc tgcattagcg
    65521 acgcttcgaa cctggaagcc ttgttcggtg agataacgtt ccagcagcgc acgcaggcgc
    65581 atgtcgtcat cgaccaccag aatcttgtag ttctcttgca ttgtttgtac tcccaaaggt
    65641 tcgcaacaat ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc agctaaatct
    65701 ggtatgaatt tcagcctaaa ttgttacaaa gcatattaaa cagcagctta agtatacaat
    65761 ttattcggcg aaacattatt gattctgttg atatgatcac gttataccca atgtgcgcat
    65821 tatcaaacag acaaagggaa tcaacgagat gaaaacgccc ctggttaccc gggaagggta
    65881 tgaaaaactc aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg aggtcacaaa
    65941 aaaggtgacc tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact atcagtataa
    66001 taaaaagcgt ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat gcctggaaaa
    66061 tctcaaaatt gtcgattact cccctcagca ggaaggcaaa gtcttttttg gcgcgtgggt
    66121 ggagattgaa aacgacgatg gcgtgactca ccgtttccgt attgtcggct acgatgaaat
    66181 ttttggccgt aaagattaca tctctatcga ttccccgatg gcccgcgcat tgctgaaaaa
    66241 agaagtcggc gatctggcgg tggtgaatac ccctgccggg gaagcgaact ggtatgttaa
    66301 tgctatcgag tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat ggctggcatt
    66361 tttgccagcc agtccgtata actatcccct gatttttgat ccgaaaagat gaactcaaac
    66421 catgatgaat gattcgttct gccgcattat tgcgggtgaa attcaggcgc gcccggaaca
    66481 ggttgacgct gccgttcgcc tgcttgatga agggaatacc gtgccgttta tcgcacgtta
    66541 tcgtaaggaa atcaccggcg gtctggatga cacgcagctg cgtaatctgg aaacgcgtct
    66601 gagctatctg cgcgagctgg aagagagacg tcaggcgatc ctcaaatcca tttccgagca
    66661 aggcaaactc accgatgatc tggcgaaggc catcaacgcc accctaagca aaaccgaact
    66721 cgaagacctc tacctgccct acaaacctaa acgccgcacc cgcgggcaaa tcgccattga
    66781 agcagggctt gagccgttgg ctgacctgct gtggagcgat ccgtcacaca cgccagaagt
    66841 cgccgctgca caatatgttg atgccgataa aggcgtggca gataccaaag ccgcgctgga
    66901 tggcgcgcgc tatatcctga tggaacggtt tgccgaagat gccgcgctgc tggcgaaagt
    66961 gcgtgattat ctgtggaaga acgcgcattt ggtttctacg gtggtgagcg gtaaagaaga
    67021 ggaaggggcg aaattccgcg actatttcga tcatcacgaa ccgttgtcca cggtgccttc
    67081 tcaccgcgcg ctggcgatgt tccgtgggcg taacgaaggc gtactccagc tttcgctgaa
    67141 tgccgatccg cagttcgatg agccgcccaa agagagctat tgcgagcaaa tcatcatgga
    67201 tcaccttggc ctgcgcctga acaatgcccc ggcggatagc tggcgcaaaa gcgtagtgag
    67261 ctggacctgg cgcatcaagg tgctgatgca tctggaaacc gaactgatgg gtaccgtgcg
    67321 cgaacgcgcg gaagatgaag caatcaacgt ctttgcccgt aacctgcacg atctgctgat
    67381 ggcggcccct gccggactgc gtgcaacgat gggcctcgat ccgggtctgc gtaccggggt
    67441 aaaagtggcg gtggtcgatg ccactggcaa actggtggcg accgatacca tttacccgca
    67501 caccggacaa gccgcaaaag cagcgatgac cgttgctgcc ttgtgtgaaa aacataacgt
    67561 tgaactggtg gcgatcggca acggtacagc ttcccgcgaa actgaacgtt tctatctcga
    67621 cgtgcagaag cagttcccga aagtgaccgc acagaaagtg atcgtcagcg aagctggcgc
    67681 gtcggtttac tcagcttccg agctggcagc gcaggagttc ccggatctcg acgtttcgct
    67741 gcgtggtgcg gtatctatcg cccgccgttt gcaggatccg ctggcggagc tggtgaaaat
    67801 cgatccgaaa tctatcggcg taggtcagta tcagcatgac gtcagccaga cgcaactggc
    67861 ccgcaaactg gatgcagtag tagaagactg cgtaaacggc gttggcgtcg atctcaacac
    67921 cgcttctgtt ccgctattaa cccgcgtggc gggcctgacg cgcatgatgg cgcaaaacat
    67981 cgtggcctgg cgcgatgaga acggccagtt ccagaaccgt cagcaactgc tgaaagtcag
    68041 ccgtctgggg ccgaaagcct tcgagcagtg cgcgggcttc ctgcgcatta accacggtga
    68101 taacccgctg gacgcctcta ccgttcatcc ggaagcctat ccggtggtgg aacgcattct
    68161 ggcagcaaca cagcaggcac tgaaagatct gatgggtaac agcagcgaac tgcgtaacct
    68221 gaaagcgtct gactttactg atgaaaaatt cggtgtgccg acagtaactg acatcatcaa
    68281 agagctggaa aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc agtttgccga
    68341 tggcgtcgag acaatgaacg acctgcaacc gggtatgatc ctcgaaggcg cagtgaccaa
    68401 cgtcaccaac tttggcgcgt ttgtcgatat tggcgtgcat caggacggcc tggttcacat
    68461 ctcttcattg tcgaacaagt ttgtggaaga tccgcatacc gtggtgaaag cgggcgacat
    68521 tgtgaaggtg aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc tgactatgcg
    68581 tctggacgag cagcctggcg aaaccaactc tcgtcgcggc ggcggtaatg aacgcccgca
    68641 aaacaaccgc ccggcagcca aaccacgcgg tcgtgaagcg cagcctgccg gtaatagcgc
    68701 gatgatggat gcgctggcgg cagcgatggg taaaaaacgt taaacgcccg gactggcccc
    68761 taaacctgcg cgataaggcg ttcacgccgc atctggcaac gaacgctttg cctgacataa
    68821 aagtgccgga gaatatctcc ggcactttta ttccacagcc aaactcacaa tatactccgt
    68881 taatatttat cacttcatta acaactgaaa ccttaattaa acattagcca gtccgggtaa
    68941 ctcactattc gaattatatt ttcgctgcga tataaccttg agccacatca acattgagtc
    69001 agattattat tcaaaccaac attcgtacac attttaagta ttgctgatag aaaccattct
    69061 cattatcatt gtgttgttga ttatttaatc tctcctttgt tggcaaatca tctggtctca
    69121 tgtcgcggtc aaacgcccca tgaggtagtt atccagttaa tgagaaacaa gtaggcacct
    69181 atgcaataca ctccagatac tgcgtggaaa atcactggct tttcccgtga aatcagcccg
    69241 gcatatcgcc aaaaactgct ttctctaggc atgttacctg gctcctcttt tgatgtggtg
    69301 cgcgtcgctc cactcggcga ccccattcat atcgaaaccc gtcgtgtgag cctggtatta
    69361 cgcaaaaaag atctggcctt attagaagtg gaagcggttt cctgttaata cggtgataac
    69421 aacaatgaaa aaattaacca ttggcttaat tggtaatcca aattctggca agacaacctt
    69481 atttaaccag ctcactggcg cacgtcagcg tgtaggtaac tgggctggcg ttaccgtcga
    69541 acgtaaagaa gggcaattct ccaccaccga tcatcaggtc acgctggtgg acctgcccgg
    69601 cacctattct ctgaccacca tctcatcgca gacctcgctc gatgagcaaa tcgcctgtca
    69661 ctacattttg agtggcgacg ccgacctgct gattaacgtg gtggatgcgt ctaaccttga
    69721 gcgtaacctg tacctgacgc tgcaactgct ggaactcggc attccctgca tcgtggcgct
    69781 gaacatgctc gacattgccg agaagcaaaa tattcgtatt gaaattgatg ctctatcggc
    69841 acgtcttggc tgcccggtga tcccgctggt ttcaacccgt ggtcgcggta ttgaagcgct
    69901 caagctggcg attgatcgct ataaagctaa cgagaatgtg gaactggtgc attacgcaca
    69961 gccgctactc aacgaagcag attcactggc aaaagtgatg ccttccgaca tcccgctgaa
    70021 acaacgtcgc tggctgggcc tgcaaatgct ggaaggcgat atctacagcc gcgcctacgc
    70081 cggtgaagcg tcgcagcatc tggatgccac cctcgcccgt ctgcgtaatg aaatggatga
    70141 tccggcgctg cacattgccg atgcgcgtta ccagtgcatt gctgccatct gtgatgtggt
    70201 aagcaacacc ctgacggcag aacccagccg tttcaccact gcggtagata aaatcgtgct
    70261 caaccgtttc ctcggtctgc cgattttcct ctttgtgatg tacctgatgt tcctgctggc
    70321 tatcaacatc ggcggggcgt tacagccgct gtttgacgtc ggctccgtgg cgctatttgt
    70381 gcatggtatt caatggattg gctacacgct ccacttcccg gactggctga ctatcttcct
    70441 cgcccagggc atgggtggcg gtattaacac cgtgctgcca ctggtgccgc agatcggcat
    70501 gatgtacctg ttcctctcct tccttgagga ctccggctat atggcgcgtg cagcgtttgt
    70561 gatggaccgc ctgatgcagg cgctgggctt gccggggaaa tcctttgtgc cgctgatcgt
    70621 cggtttcggt tgtaacgtac cgtcggtaat gggcgcacgt acgcttgatg caccgcgtga
    70681 acgtctgatg accatcatga tggcaccgtt tatgtcctgc ggcgcgcgtc tggctatctt
    70741 cgcagtattc gcggctgcct tcttcgggca gaacggtgcc ctggcggttt tctcgctgta
    70801 tatgctcggt atcgtgatgg cggtgctgac tggcctgatg ctcaagtaca ccatcatgcg
    70861 cggtgaagca acgccgtttg tcatggagct gccggtatac catgtaccac acgttaaaag
    70921 cctgattatc cagacctggc agcgtctgaa gggcttcgtt ctgcgtgctg gtaaagtgat
    70981 tatcatcgtc agcattttcc tgagcgcttt caacagcttc tcgttgagcg ggaaaatcgt
    71041 cgataacatc aacgactcgg cgctggcttc cgtcagccgg gtgataaccc ctgtcttcaa
    71101 gccgattggc gtacatgaag ataactggca ggcaacggtt ggcctgttta cgggcgcaat
    71161 ggcaaaagaa gtggtggtgg gtacgctcaa caccctctac accgcagaaa atattcagga
    71221 cgaagagttc aatccggcgg aattcaacct cggtgaagag ctgttcagtg cggtagatga
    71281 aacctggcag agcctgaaag acaccttcag ccttagcgta ctgatgaacc ccattgaagc
    71341 tagcaaaggc gacggtgaaa tgggtaccgg ggcgatgggc gtgatggatc agaaattcgg
    71401 tagcgcagct gccgcataca gctacctgat tttcgtcctg ctgtatgtac catgtatctc
    71461 ggtgatggga gccatcgccc gtgaatcaag ccgtggctgg atgggcttct ccatcctgtg
    71521 ggggctgaat atcgcttact cactggcaac attgttctat caggtcgcca gctacagtca
    71581 gcacccaact tacagcctgg tgtgcattct ggcggtaatc ctgtttaaca tcgtagttat
    71641 cggtctgctg cgccgcgcgc gtagccgggt ggatatcgaa ctgctggcaa cccgcaagtc
    71701 ggtaagcagt tgctgcgcag ccagcaccac cggtgattgc cattaatggc ttcacttatt
    71761 caggtacgtg atttgctggc gttacggggc cgtatggaag cgacccagat aagccagaca
    71821 ttgaacactc cgcagccaat gattaacgcc atgctgcaac agctggaaag catgggcaaa
    71881 gccgtgcgga ttcaggaaga acctgacggc tgcctctctg gcagttgtaa aagctgcccg
    71941 gaaggcaaag cctgtctgcg cgagtggtgg gcgctgcgtt aaccatactc catcgcctgt
    72001 tttgaaaagc ccggtatgcg tttgcatccg ggcttttttg cgtgcggctt tccataaaaa
    72061 tgcaattctt gcagcgcaac gtaagttcct ctgaaagcgt ctcgcaggga tgaaaactcg
    72121 ctaatgcaca gatgtggagt ggcgcgtaga gtcgcggcat tcaaacaaca ggtgaaggaa
    72181 cgccatgagt aaaaagcaga gttccacccc acatgatgcg ctgttcaaac tctttttacg
    72241 ccaaccggag acggcacgcg attttcttgc gtttcattta ccggcaccca ttcacgcgct
    72301 ctgtgatatg aaaaccctca agctggagtc gagcagcttt attgatgacg atctgcgtga
    72361 aagctattcc gatgtgctgt ggtcggtgaa aacggaacaa ggaccaggat acatctattg
    72421 tctgattgaa catcaaagca cctcaaacaa actgatcgca tttcgcatga tgcgttacgc
    72481 tattgccgca atgcaaaatc accttgatgc tggatacaaa acgttgccga tggtggtgcc
    72541 attgttgttt taccacggta ttgaaagccc ctatccctat tcgctgtgtt ggctggattg
    72601 tttcgccgat cccaaactgg caaggcagct ttatgcctcc gcatttccgc tgattgatgt
    72661 caccgtcatg cctgatgatg aaatcatgca gcaccgacgt atggcgctgc tggagttaat
    72721 tcaaaaacat attcgtcaac gcgatctgat ggggctggta gagcaaatgg cctgcttatt
    72781 aagtagtgga tacgctaatg acagacaaat caaagggctg tttaattaca tactgcaaac
    72841 tggcgacgct gtacgtttta acgattttat cgacggcgtt gccgaacgtt caccgaaaca
    72901 caaggagagt ttaatgacta ttgcggaaag attgcggcag gagggggaac aatccaaagc
    72961 cctgcatata gccaaaataa tgcttgaatc cggagtccct cttgcagaca tcatgcgatt
    73021 taccgggctg tcagaagaag agttggctgc ggcgagtcag tagatactct accctgctta
    73081 agtgctactt tgtgctatct tgcatgtaac acaaagtagc acttagggaa ctgatatgca
    73141 gacagtgaaa ttacggcaac aaggaggagc catgatcgtt actatcccac gagatcttgc
    73201 catcgatctt ggctggtcac cgggcacaga gcttaccgtg gagaaaaaag gcgatagcgt
    73261 taatttgcgc gccacagagc acaaaccgcg tgggcgtctt accgtcgcgc agttgttgag
    73321 ccaaatcgat gaaagcgaaa ttacggagct aaatcagtcc acagaaggat gggctgaagg
    73381 gaaaaaaggt aatgaagcat ggtaaaggca cggacgccac atcgtggtga gatctggtat
    73441 tttaaccctg atccggttgc cgggcatgaa cttcaggggc cacattattg cattgtggta
    73501 acggacaaaa aactcaacaa tgttttaaaa gttgctatgt gctgcccgat ttcaacaggg
    73561 gcaaatgcag cacgttccac aggggtgacg gtgaacgtcc tcccctgtga tacgcaaacc
    73621 ggtaacctgc atggcgttgt actttgtcac cagctaaaag ccgtcgatct tattgcccgt
    73681 ggcgctaaat ttcataccgt tgccgatgaa aaattgatta gtgaagttat cagtaaactg
    73741 gtgaatttaa tcgacccaca ataattagct ctcacttacc accaacaccc tctgcttcaa
    73801 cgccaccagc aggtgacaaa actcgaccgg atgcgaaata aacggcgcat gggccgcttt
    73861 ggcgaagata tatgattcgc tgtgaggcca aagtttatcc agcatcggca ccactttgcg
    73921 cggcaccaga ccgtcgagat agccatacaa tcgcaaaaac ggcatggaca cgttttgcag
    73981 cggctgacgg agatcgaccg ttttcaggat ttccagcccg ccattaagca cgtcaacctc
    74041 cggcatcggt aacgccagaa cggttttctt caacgcccgc gcatcctggc gcgccgtttc
    74101 agtccccatg gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat catcactgag
    74161 ttgctgctga aatcccgcca gcacgtccgg ttttatcccc ggccactcgt cacgagcact
    74221 aaaataaggt gacgacgcca cggtgaccag cgcctgaaca cgctcgggat gggttaacgc
    74281 aatctggctt gccaccagcc cgcccagact ccagcctaac caaatggctt tatcaggtgc
    74341 ctgttgcagc acggcttcgg ccatatcagc aagtgacagc gcaccaaatc cccggctacg
    74401 cccgaagccg ggcaggtcaa caaggtgcag cgtaaaatgc gagctaagtt cctcgtcaat
    74461 gcaacgccac acttcggcat tcagtcccca tccgtgcagc agcacaagat gaacattccc
    74521 ctgacctttg gtctgccacc agatgttatt catccgctat tgttctcttt tgacttacaa
    74581 ggatgaacat atgctaacag taccgggatt atgctggcta tgccgaatgc cactggcgtt
    74641 aggtcattgg gggatttgtt cggtctgttc acgcgccacc cgcacagata aaacgttatg
    74701 cccacaatgt ggattacccg ccacacactc ccatcttccc tgcggtcgct gcctgcaaaa
    74761 accgccgccc tggcaaagac tggtcacggt tgccgactat gcgccgccgt taagtccgct
    74821 tatccaccag cttaaatttt cccggcgcag tgaaatcgcc agcgccctgt cacgtctgtt
    74881 actattggaa gtcttacacg ctcgtcgcac caccgggttg caattgccgg atcgcatcat
    74941 cagcgttccg ttatggcagc ggcgtcactg gcgtcgggga tttaatcaga gcgatttgct
    75001 gtgtcagccg ttatcacgct ggttgcactg ccaatgggat agcgaagccg tcacacgtac
    75061 acgggccact gcgacccagc attttctcag tgcccggctg cgcaagcgca acctgaaaaa
    75121 tgcctttcgt cttgaattgc ccgtgcaagg tcgccatatg gtgattgtgg atgatgtcgt
    75181 taccaccgga agtaccgtcg cagagattgc gcagttgctt ttacgcaatg gtgcggcgac
    75241 tgtccaggtc tggtgccttt gtcgaacctt gtagagcctc gatgatgggc gtattataac
    75301 caactaaaat agtcaactat taggccatta ctatgatccg tatttccgat gctgcacaag
    75361 cgcactttgc caaactgctg gcaaatcagg aagaagggac acaaatccgc gtatttgtga
    75421 ttaaccctgg cacgcctaac gctgaatgtg gtgtttctta ttgtccgccg gatgccgtgg
    75481 aagccaccga cactgccctg aaatttgacc tgctgaccgc gtatgttgat gagttaagcg
    75541 caccatacct ggaagatgca gagatcgact ttgttaccga ccagttgggt tctcagttaa
    75601 cgctgaaagc cccgaacgcc aaaatgcgta aagtggcaga cgatgcaccg ctgatggagc
    75661 gcgtggagta tatgctgcag tcgcagatca acccacagct tgctggtcac ggtggtcgcg
    75721 tttcgctgat ggaaatcacc gaagacggtt acgccattct gcaatttggc ggcggctgta
    75781 acggttgttc catggtcgat gtgacgctga aagaagggat cgagaagcag ctgctgaacg
    75841 aattcccgga gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc gaacactcct
    75901 actactaagt tatcccctct tttgtagatt gcccgatgcg acgctaaagc gtcatttcgg
    75961 gcctacgttg aacatgtgcc agacgttggc acagcattgc tttggcaaat ccctcccttc
    76021 gtttccccgc gataatatga ccaacctctc ataatttaaa tttaccccgc tctggtgatt
    76081 ctcaaacgcc agatgttacc cgtatcattc acatgggtac caaacatact cctgacatct
    76141 gactacaata attagtttta gtgggtatca gtcgtggtgc cgcaatatct ctgttcccga
    76201 ttgggataat tagagtttgt cgtcagaaaa ttgacattac ccataacaaa tgaaaggcca
    76261 ggtaaatcat gccattagtc attgttgcta tcggtgtaat cttgttgttg ctcctgatga
    76321 tccgcttcaa aatgaacggc ttcatcgctc tcgtcctcgt ggcgcttgct gttggattaa
    76381 tgcaaggaat gccgctggat aaagttattg gctccatcaa agccggtgtc ggcgggacgc
    76441 tcggtagcct tgccctgatc atgggttttg gcgcaatgct ggcaaactgc ggtggcgcac
    76501 aacgtatcgc caccacgctg attgccaaat ttggtaaaaa gcacatccag tgggcggtgg
    76561 tgttaaccgg ttttaccgtt ggttttgccc tgttctatga agtgggcttc gtgctgatgc
    76621 tgccgctggt gtttaccatc gcggcttccg cgaatattcc attgctgtat gttggtgtac
    76681 caatggcggc tgcactgtct gtgacccacg gcttcctgcc accgcatccg ggcccgactg
    76741 cgattgccac cattttcaat gccgatatgg gtaaaaccct gctgtacggt actattctgg
    76801 caatcccgac cgtgattctc gccggtccgg tttacgctcg cgtgttgaaa ggtatcgata
    76861 agccaatccc ggaaggtctc tacagcgcga aaaccttcag cgaagaagag atgccgagct
    76921 ttggcgtcag cgtctggacc tctctggtgc cggttgtact gatggcgatg cgtgcgattg
    76981 ccgaaatgat cctgccgaaa ggtcacgctt tcctgccggt agcggagttc ctcggtgacc
    77041 cggtaatggc aacgctgatt gccgtgctga ttgcgatgtt cacctttggt ctgaaccgtg
    77101 gtcgttcaat ggatcagatt aacgacacgc tggtttcttc catcaaaatc attgcgatga
    77161 tgctgttgat catcggtggt ggcggtgcgt tcaagcaggt gctggtagac agcggcgtgg
    77221 acaaatacat tgcttccatg atgcacgaaa ccaacatttc tccgctgctg atggcctggt
    77281 cgattgctgc cgtactgcgt atcgcgctgg gttccgcaac cgttgcggca atcactgcgg
    77341 gtggtatcgc ggcaccgctg attgcaacga cgggtgttag cccagagctg atggttattg
    77401 cggtgggttc cggtagtgtg attttctctc atgtgaacga tccgggcttc tggctgttca
    77461 aagagtactt taacctgact atcggcgaga ccatcaaatc ctggtcgatg ctggaaacca
    77521 ttatctcggt gtgcggtctg gtaggctgtc tgctgctgaa tatggtgatt tgataacact
    77581 gactgccgga tgcatcgcat ccgacatcaa ccgcactcta cttcttcttc gctgcagctc
    77641 tgcgccgtct gtccaaatcc ttcagcaact tgttcacgcc atcatcggca aacatcgact
    77701 caagcgttgc ggaaagcttg cgtcgccagt ttttatactg gtaactggtg ccaggaatat
    77761 tcaccggttc ggccatatcc agccagtctt ccggctgtag tcctaacaga gcactgttac
    77821 tgtcggcaat gtagcgctgc aaaccacggt tcagcgtcgt cgtcatcgac atcaacgatg
    77881 ccttatgccc ggcacgtttt ggcagacaac catatttatg cagtgcatcc agcagccctt
    77941 gcttcgccag ttcgcgatcc tgatacagac cgcgcagtac cacttcatcc ggatacagcc
    78001 ccagggtttt gcccagcgtt agatccccgc tctcccagta accacgcagc gttggcaggt
    78061 catgtgtcgc cgcaaccgcc atcgactgct ccggatacgc tttcggtgca cggaacgtct
    78121 tctcgtggtc gttttcgaaa tagagcactt tgtaagagta cacaccgctg ctgcgcagct
    78181 taccgacaat ctctaccggt acggtaccga gatcttcacc aatcaccata cagcgatgac
    78241 gtttactttc gagtgccaga atcgagagca gatcatccac cggatagtga acatacgcgc
    78301 cctgatctgc cgtctcgcca tacggtatcc accacaaacg cagcatcgac atcacatggt
    78361 caattcgtaa tgcgccgcag ttttgcatat tggcacgcaa cagctcgata aacggttcat
    78421 aggcacgcgc ggtgatgata tgcgggtcca ttggcggtaa tccccagttc tgccccaacg
    78481 ggccgaggat atccggcggc gcgccaaccg atgctttcag gcaatatagt tcacggtcac
    78541 accaggtttc cgccccacct tccgctacgc caaccgccag atcacgatac aagccaatcg
    78601 gcatttcata gccctggctt atctcccagc aggcggcaaa ctggctgtaa gccagccact
    78661 gcaaccagag ataaaaatcg acgtcatcac gatgttcttc gcagaactga cgcacttctg
    78721 gtgaatccac gttcttatac atctctggcc atgcaggcca gccccagcgc atttcgtcct
    78781 ctttcacttg ctgggcatgt agcgcatcaa aggctgcctg ccagaacagg ctgtcgccct
    78841 gctctgcaac aaactggcga aacgcggcca tctgctcatc atcacgttgc gcgaaacctt
    78901 tccacgccat tcgtaatgct gtcattttta gggcggtaac cgtggagtaa tcgacccagt
    78961 cggcatcgcg cgcctgttgc agcgtctgtt gcgtggtcgg caactgccac caggcctgag
    79021 cctcttcgct aagatggaaa tcttcaacgg cgttaacgtc gatataaatc acattcagcc
    79081 aacggcgaga agacgggctg tatgggctgg cgctctccgg atttgccgga tagagcgcat
    79141 gaatcgggtt caggccaatg aacgacccgc cacgttttgc cacatccacc agcatcgctt
    79201 tgagatcgcc aaaatcccca ataccccagt ttttttccga tcgcagcgta taaagctgaa
    79261 cgcaggcacc ccacagcttt tgtttattca gcaaagcctg cggttcgtaa caacgtttcg
    79321 gggcgacaat cacccggcaa tgcgcgcgct ggtcgtcctg ggtgagtgtt agcgtgtgat
    79381 aaccttccgg cagcttcgtc ggtagattga acgctttgcc ccccgttaca tggcctttgt
    79441 actgcgttcc ttcttcggtg gtcagctgcc agctatattc gccgctgccc tccaccacca
    79501 tcggcatttt tttgccgctg gtataaacca tgacattcgg gactggcgtt accgccactt
    79561 tcgtggcggt acgttgatgc atcgcgtcaa gcaaacgccg tttggtttcg gcgctaatcg
    79621 actgcggttt accgtgggca ttgatgtaat tggggctaat ccccgccgcc agcgcggcat
    79681 tatccagacg tttgctttcc atcgagcttc cttagcgttt tgcctgccag atacgagcct
    79741 gataatcgcg aatagagcga tccgagctaa acataccgca gcgggcggta ttgaggatcg
    79801 ccgcgcgagt ccaggcctcc tggtcgcggt acagcacatc cacctgcttt tgtgcctcaa
    79861 cataggctgc gaaatccgcc atcaccagat acggatcgcc gccctgtttg ccgatactgt
    79921 gcagcatctg gtcgaaggca tgcttatcgc cgtcgctgta tttaccgctt tccagctctt
    79981 tcaatactgc gtccagcacc ttatctttct tccgccattt caccgggtcg tagcctttgg
    80041 ccagaatagc cttcacttgt tccacggtat gaccaaaaat aaagatattt tcttcaccga
    80101 ctttctcggc gatttcaacg ttcgcaccat ccagcgtacc gacagtaagc gcaccattga
    80161 gcgccagttt catattgccg gtaccggaag cttctttacc tgcagtcgaa atttgttcgg
    80221 agatatccgc cgccgggatc agtttttccg ccgccgaaac gcaataatcc ggcaggaaca
    80281 ccaccttcaa cttatcgcca accagtggat cgttgttgat cacgtcagcc actttgttga
    80341 tcgcaaagat aatattcttc gccaggtagt agcccggtgc cgctttcgcg ccgaagagga
    80401 agacgcgcgg tacgcgatca gcctgcaggt tttcacgaat ttctttgtac aacgccagaa
    80461 tatgcagcag attcaggtgc tggcgtttgt actcatgcag acgtttgatc tgaatatcga
    80521 aaatcgcctg tggattgatc tcaataccgg tacgaacttt cacaaactcc gccagacgga
    80581 ctttattcgc ctgcttgatc tcgcgatatt gctgacggaa tttcgcatca tcagcgaatt
    80641 tttccagatt gatcagctga tcgagatcgt tagcccactc tttttgcagt gatttatcca
    80701 acagagccgc cagtgcaggg ttgcactgtt tgatccagcg acgtggggta ataccgttgg
    80761 tgacgttatg gaatttgttc ggccatagct ggtgatattc cgggaacaga tctttcacca
    80821 ccagatccga gtgcagcgcc gcaacaccgt tcaccgcgaa accgccaacc acacacaggt
    80881 tcgccatatg cacttgttta tcgtgcacca ccgccagttt ggcccacact ttttcatcgc
    80941 ccggccaggt tttctccacc agcgttttaa agcgagtatt aatttcgtta ataatctgca
    81001 tatggcgcgg cagtaagcct ttcaccagtt tcacatccca gcgttccagc gcttctggca
    81061 tcagggtatg gttggtgtaa gcgaaagttt tgctggtgat ggcccaggcg tcatcccagc
    81121 tcatctggtg ctcatcggtc agcacgcgca gcagttctgg aatcgcgata gttggatggg
    81181 tatcgttcag ctgaataact tcgtaatccg ccagttcgtg cagtttacgc cccgccagat
    81241 gatggcgacg caaaatatcc gctaccgaac aggcacactg gaagtattgc tgcatcaggc
    81301 gcagcttttt accggcagta tggttgtcgt ttggatagag aactttggtc agtttttccg
    81361 cattgatgcc ttgctgttcg gcgcgcagga aatcaccgtc gttaaattta gttagatcaa
    81421 acggatgcgc atgcgtcgcc tgccacagac gcagcggctg cgccacgcca ttacgatagc
    81481 cgacaacggg gagatcccac gcttgaccgg taatggtaaa ctccggctcc cagcgtccgt
    81541 ctttcatcac tttaccgcca atccctacct gcacatccag tgcttcgttg tggcggaacc
    81601 acgggtagtt actgcgatgc cagtcatccg gcgcttcaac ctgtttgcca tcgacaaaag
    81661 actggcggaa caaaccatat tgatagttca gaccgtaacc cgtcgcagac tgaccgacag
    81721 ttgccattga gtcgaggaag cacgccgcca gacgtcccag accaccgtta cccagcgccg
    81781 ggtcgatctc ttcttccagc aggtcagtca ggttgatgtc ataagccttc aacgaatcct
    81841 gtacatcctg ataccagccg agattcaaca ggttgttgcc cgtcagacga ccaatcaaaa
    81901 actccattga gatgtagtta acatgtcgct gattcgccac cggtttggcg aatggctgag
    81961 cacgcagcat ttcggccagt gcttcactca ctgccagcca ccactggcga ggagtcattt
    82021 cagccgcaga atttaagcca taacgctgcc actgacgtga aagcgcttcc tgaaattgct
    82081 tatcgttaaa aataggttgt gacataggag ttccactttt cttagatttt caacacaacg
    82141 ttatcgctag tttgccaggc tcgatgttga ccttcctcat cctgcggggg attaggcagg
    82201 gaggagttgc ggggatgagc aaggaaatgt gatctcgacc acttaaagct agtgcaaacc
    82261 acaggattag cagcaaatca atgccataca gcgcagaaaa tctgtatcta agtgcaaaaa
    82321 atggccgttg cgtattttca aaaagcggaa ggtaactcta taaattaagt aaaggagtga
    82381 aacagtttca caagtaaaat atccagtgtg ctccatctca ttcttaataa atttattaag
    82441 atcatctttt tagatggcac tttcatcagg aatgaagaag aaacccttgc ttaaatgaat
    82501 ctgatg
//
LOCUS       DAJTYF010000005        68857 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTYF010000005 DAJTYF010000000
VERSION     DAJTYF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.005"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..178
                     /locus_tag="OO590_000419"
     CDS             <1..178
                     /locus_tag="OO590_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6635914.1"
                     /translation="IENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGELET
                     GKFQGTATITVMVR"
     gene            complement(221..1081)
                     /gene="rsmI"
                     /locus_tag="OO590_000420"
     CDS             complement(221..1081)
                     /gene="rsmI"
                     /locus_tag="OO590_000420"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6635915.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1146..3182
                     /gene="lpoA"
                     /locus_tag="OO590_000421"
     CDS             1146..3182
                     /gene="lpoA"
                     /locus_tag="OO590_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6635916.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLALQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3140..3535
                     /locus_tag="OO590_000422"
     CDS             3140..3535
                     /locus_tag="OO590_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6635917.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3555..4145
                     /gene="diaA"
                     /locus_tag="OO590_000423"
     CDS             3555..4145
                     /gene="diaA"
                     /locus_tag="OO590_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6635918.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4155..4730
                     /gene="yraP"
                     /locus_tag="OO590_000424"
     CDS             4155..4730
                     /gene="yraP"
                     /locus_tag="OO590_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6635919.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4844..5884)
                     /locus_tag="OO590_000425"
     CDS             complement(4844..5884)
                     /locus_tag="OO590_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6635920.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLPGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5957..6592)
                     /locus_tag="OO590_000426"
     CDS             complement(5957..6592)
                     /locus_tag="OO590_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6635921.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6720..7238
                     /gene="yhbO"
                     /locus_tag="OO590_000427"
     CDS             6720..7238
                     /gene="yhbO"
                     /locus_tag="OO590_000427"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6635922.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7218..7661)
                     /locus_tag="OO590_000428"
     CDS             complement(7218..7661)
                     /locus_tag="OO590_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6635923.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7712..8014
                     /gene="yhbQ"
                     /locus_tag="OO590_000429"
     CDS             7712..8014
                     /gene="yhbQ"
                     /locus_tag="OO590_000429"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6635924.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8001..8504)
                     /locus_tag="OO590_000430"
     CDS             complement(8001..8504)
                     /locus_tag="OO590_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6635925.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8498..9022)
                     /locus_tag="OO590_000431"
     CDS             complement(8498..9022)
                     /locus_tag="OO590_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6635926.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVCDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9231..10226
                     /locus_tag="OO590_000432"
     CDS             9231..10226
                     /locus_tag="OO590_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6635927.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10235..11113
                     /locus_tag="OO590_000433"
     CDS             10235..11113
                     /locus_tag="OO590_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6635928.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11194..12201
                     /locus_tag="OO590_000434"
     CDS             11194..12201
                     /locus_tag="OO590_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6635929.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12319..13563)
                     /gene="mtr"
                     /locus_tag="OO590_000435"
     CDS             complement(12319..13563)
                     /gene="mtr"
                     /locus_tag="OO590_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6635930.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13717..15606)
                     /gene="deaD"
                     /locus_tag="OO590_000436"
     CDS             complement(13717..15606)
                     /gene="deaD"
                     /locus_tag="OO590_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6635931.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15599..15679)
                     /gene="yrbN"
                     /locus_tag="OO590_000437"
     CDS             complement(15599..15679)
                     /gene="yrbN"
                     /locus_tag="OO590_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6635932.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15786..16670)
                     /gene="nlpI"
                     /locus_tag="OO590_000438"
     CDS             complement(15786..16670)
                     /gene="nlpI"
                     /locus_tag="OO590_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6635933.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEHTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16779..18914)
                     /gene="pnp"
                     /locus_tag="OO590_000439"
     CDS             complement(16779..18914)
                     /gene="pnp"
                     /locus_tag="OO590_000439"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6635934.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19161..19430)
                     /gene="rpsO"
                     /locus_tag="OO590_000440"
     CDS             complement(19161..19430)
                     /gene="rpsO"
                     /locus_tag="OO590_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6635935.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19579..20523)
                     /gene="truB"
                     /locus_tag="OO590_000441"
     CDS             complement(19579..20523)
                     /gene="truB"
                     /locus_tag="OO590_000441"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6635936.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20523..20924)
                     /gene="rbfA"
                     /locus_tag="OO590_000442"
     CDS             complement(20523..20924)
                     /gene="rbfA"
                     /locus_tag="OO590_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6635937.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21088..23736)
                     /gene="infB"
                     /locus_tag="OO590_000443"
     CDS             complement(21088..23736)
                     /gene="infB"
                     /locus_tag="OO590_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6635938.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23761..25248)
                     /gene="nusA"
                     /locus_tag="OO590_000444"
     CDS             complement(23761..25248)
                     /gene="nusA"
                     /locus_tag="OO590_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6635939.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25276..25728)
                     /gene="rimP"
                     /locus_tag="OO590_000445"
     CDS             complement(25276..25728)
                     /gene="rimP"
                     /locus_tag="OO590_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6635940.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25935..26011)
                     /locus_tag="OO590_000446"
     tRNA            complement(25935..26011)
                     /locus_tag="OO590_000446"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25975..25977),aa:Met,seq:cat)
     gene            26359..27702
                     /gene="argG"
                     /locus_tag="OO590_000447"
     CDS             26359..27702
                     /gene="argG"
                     /locus_tag="OO590_000447"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6635941.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27710..29335)
                     /locus_tag="OO590_000448"
                     /pseudo
     CDS             complement(27710..29335)
                     /locus_tag="OO590_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(29794..29880)
                     /locus_tag="OO590_000449"
     tRNA            complement(29794..29880)
                     /locus_tag="OO590_000449"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29844..29846),aa:Leu,seq:gag)
     gene            complement(29895..30227)
                     /gene="secG"
                     /locus_tag="OO590_000450"
     CDS             complement(29895..30227)
                     /gene="secG"
                     /locus_tag="OO590_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6635942.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30455..31792)
                     /gene="glmM"
                     /locus_tag="OO590_000451"
     CDS             complement(30455..31792)
                     /gene="glmM"
                     /locus_tag="OO590_000451"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6635943.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRVLQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31785..32633)
                     /gene="folP"
                     /locus_tag="OO590_000452"
     CDS             complement(31785..32633)
                     /gene="folP"
                     /locus_tag="OO590_000452"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6635944.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32723..34657)
                     /gene="ftsH"
                     /locus_tag="OO590_000453"
     CDS             complement(32723..34657)
                     /gene="ftsH"
                     /locus_tag="OO590_000453"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6635945.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34757..35386)
                     /gene="rlmE"
                     /locus_tag="OO590_000454"
     CDS             complement(34757..35386)
                     /gene="rlmE"
                     /locus_tag="OO590_000454"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6635946.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35512..35805
                     /gene="yhbY"
                     /locus_tag="OO590_000455"
     CDS             35512..35805
                     /gene="yhbY"
                     /locus_tag="OO590_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6635947.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKKRKISLPR"
     gene            complement(35961..36437)
                     /gene="greA"
                     /locus_tag="OO590_000456"
     CDS             complement(35961..36437)
                     /gene="greA"
                     /locus_tag="OO590_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6635948.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36685..38118
                     /gene="dacB"
                     /locus_tag="OO590_000457"
     CDS             36685..38118
                     /gene="dacB"
                     /locus_tag="OO590_000457"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6635949.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38304..39476)
                     /gene="cgtA"
                     /locus_tag="OO590_000458"
     CDS             complement(38304..39476)
                     /gene="cgtA"
                     /locus_tag="OO590_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6635950.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39492..40457)
                     /locus_tag="OO590_000459"
     CDS             complement(39492..40457)
                     /locus_tag="OO590_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6635951.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40584..40841)
                     /gene="rpmA"
                     /locus_tag="OO590_000460"
     CDS             complement(40584..40841)
                     /gene="rpmA"
                     /locus_tag="OO590_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6635952.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40862..41173)
                     /gene="rplU"
                     /locus_tag="OO590_000461"
     CDS             complement(40862..41173)
                     /gene="rplU"
                     /locus_tag="OO590_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6635953.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41432..42403
                     /gene="ispB"
                     /locus_tag="OO590_000462"
     CDS             41432..42403
                     /gene="ispB"
                     /locus_tag="OO590_000462"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6635954.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42631..42909
                     /gene="sfsB"
                     /locus_tag="OO590_000463"
     CDS             42631..42909
                     /gene="sfsB"
                     /locus_tag="OO590_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6635955.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(42957..44216)
                     /gene="murA"
                     /locus_tag="OO590_000464"
     CDS             complement(42957..44216)
                     /gene="murA"
                     /locus_tag="OO590_000464"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6635956.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44271..44525)
                     /gene="ibaG"
                     /locus_tag="OO590_000465"
     CDS             complement(44271..44525)
                     /gene="ibaG"
                     /locus_tag="OO590_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6635957.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44685..44978)
                     /gene="mlaB"
                     /locus_tag="OO590_000466"
     CDS             complement(44685..44978)
                     /gene="mlaB"
                     /locus_tag="OO590_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6635958.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44978..45613)
                     /gene="mlaC"
                     /locus_tag="OO590_000467"
     CDS             complement(44978..45613)
                     /gene="mlaC"
                     /locus_tag="OO590_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6635959.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45632..46183)
                     /gene="mlaD"
                     /locus_tag="OO590_000468"
     CDS             complement(45632..46183)
                     /gene="mlaD"
                     /locus_tag="OO590_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6635960.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46188..46970)
                     /gene="mlaE"
                     /locus_tag="OO590_000469"
     CDS             complement(46188..46970)
                     /gene="mlaE"
                     /locus_tag="OO590_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6635961.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46978..47787)
                     /gene="mlaF"
                     /locus_tag="OO590_000470"
     CDS             complement(46978..47787)
                     /gene="mlaF"
                     /locus_tag="OO590_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6635962.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            47997..48962
                     /locus_tag="OO590_000471"
     CDS             47997..48962
                     /locus_tag="OO590_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6635963.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            48976..49962
                     /gene="kdsD"
                     /locus_tag="OO590_000472"
     CDS             48976..49962
                     /gene="kdsD"
                     /locus_tag="OO590_000472"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6635964.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49983..50549
                     /gene="kdsC"
                     /locus_tag="OO590_000473"
     CDS             49983..50549
                     /gene="kdsC"
                     /locus_tag="OO590_000473"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6635965.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50546..51121
                     /gene="lptC"
                     /locus_tag="OO590_000474"
     CDS             50546..51121
                     /gene="lptC"
                     /locus_tag="OO590_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6635966.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51090..51647
                     /gene="lptA"
                     /locus_tag="OO590_000475"
     CDS             51090..51647
                     /gene="lptA"
                     /locus_tag="OO590_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6635967.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51654..52379
                     /gene="lptB"
                     /locus_tag="OO590_000476"
     CDS             51654..52379
                     /gene="lptB"
                     /locus_tag="OO590_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6635968.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52427..53860
                     /gene="rpoN"
                     /locus_tag="OO590_000477"
     CDS             52427..53860
                     /gene="rpoN"
                     /locus_tag="OO590_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6635969.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53883..54170
                     /gene="hpf"
                     /locus_tag="OO590_000478"
     CDS             53883..54170
                     /gene="hpf"
                     /locus_tag="OO590_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6635970.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54288..54779
                     /gene="ptsN"
                     /locus_tag="OO590_000479"
     CDS             54288..54779
                     /gene="ptsN"
                     /locus_tag="OO590_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6635971.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54825..55679
                     /gene="rapZ"
                     /locus_tag="OO590_000480"
     CDS             54825..55679
                     /gene="rapZ"
                     /locus_tag="OO590_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6635972.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55676..55948
                     /gene="npr"
                     /locus_tag="OO590_000481"
     CDS             55676..55948
                     /gene="npr"
                     /locus_tag="OO590_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6635973.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56161..56793
                     /gene="yrbL"
                     /locus_tag="OO590_000482"
     CDS             56161..56793
                     /gene="yrbL"
                     /locus_tag="OO590_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6635974.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56790..57518)
                     /gene="mtgA"
                     /locus_tag="OO590_000483"
     CDS             complement(56790..57518)
                     /gene="mtgA"
                     /locus_tag="OO590_000483"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR6635975.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57515..58168)
                     /gene="elbB"
                     /locus_tag="OO590_000484"
     CDS             complement(57515..58168)
                     /gene="elbB"
                     /locus_tag="OO590_000484"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6635976.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58398..60734)
                     /gene="arcB"
                     /locus_tag="OO590_000485"
     CDS             complement(58398..60734)
                     /gene="arcB"
                     /locus_tag="OO590_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6635977.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEKSTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60830..61759)
                     /locus_tag="OO590_000486"
     CDS             complement(60830..61759)
                     /locus_tag="OO590_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6635978.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSITAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62434..66894
                     /gene="gltB"
                     /locus_tag="OO590_000487"
     CDS             62434..66894
                     /gene="gltB"
                     /locus_tag="OO590_000487"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6635979.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPTTTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66907..68325
                     /gene="gltD"
                     /locus_tag="OO590_000488"
     CDS             66907..68325
                     /gene="gltD"
                     /locus_tag="OO590_000488"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6635980.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68509..>68857
                     /locus_tag="OO590_000489"
     CDS             68509..>68857
                     /locus_tag="OO590_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635981.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIP"
BASE COUNT        16815 a        18721 c        17118 g        16203 t
ORIGIN      
        1 tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta tccttcccgt
       61 tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc tggtcggtgg
      121 tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg tgcggtaaaa
      181 gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc tgctccagcg
      241 catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc gccgccgctt
      301 ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg gcgggtaagt
      361 cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg cgacggtttt
      421 catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg gtttcccagg
      481 ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc gcaacgatat
      541 cttccaggct atctaacaga cggtgggtag attcataaaa aatcagcgtg cgcggctccg
      601 cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa aagccttcgt
      661 aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca gcacacggtc
      721 cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga tggtagccag
      781 gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc ccctcttgca
      841 gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc gcaaacaacc
      901 gggcattaat cccaaaatgt tgcagcaata aaccggtgtg acgagtatcc tcggcggcaa
      961 tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc agattgccga
     1021 ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg tgttgtttca
     1081 ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat atcactggat
     1141 acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc
     1201 tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc actgcttata
     1261 tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag caaagctctg
     1321 atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa gaaggtaaaa
     1381 ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat tctcagcgtc
     1441 gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt gctggcgcgc
     1501 aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa gcgcgttact
     1561 ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg cgcgcgttaa
     1621 ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat gccacctggc
     1681 aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac gccgacgaaa
     1741 atattctgca aggctggctg gctctgcagc gcgtctggtt tgataaccgt aacgatcccg
     1801 acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat ccgggcgcga
     1861 aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg accaacaaaa
     1921 tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact attcagcaag
     1981 gctttgaagc cgcgaaaaat atcggcactc agccagtggc ggctcaggta gctgccgcac
     2041 ctgccgcaga cgtagcagaa caacctcagc cgcaaactgc ggatggcgtt gccagcccgg
     2101 cacaagcctc ggttagcgat ctgaccggtg atcagcctgc ggcccagccg gtgcctgtaa
     2161 gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc gcagagctga
     2221 aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt cagcaggatg
     2281 gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg ctgaagagca
     2341 acaccccgct gagcgtactg gcactgaacc agccggagaa tatcgaaaac cgcgtcaata
     2401 tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg
     2461 accagggtaa acaagcgccg ctggtgctga tcccacgcag tgcattgggc gatcgcgtag
     2521 ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg caacaaaaat
     2581 ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt gctttaacgg
     2641 gtagcccgat tactcccaga gcgacaaccg actccggcat gacgaccaac aatccaacgc
     2701 tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc gatgcggtct
     2761 acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc atgcgtaacg
     2821 gtagccagag cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc accgcaggcc
     2881 cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg ctggcaggcg
     2941 gtaatctgcc gttaatgcag caggcgctca gcgcggtgaa caacgattat tcgctggctc
     3001 gcatgtatgc gatgggcgtt gatgcctggt cgttggcaaa tcatttctca caaatgcgcc
     3061 aggttcaggg ttttgaaatc aacggtaata ccggaagtct gacggctaac ccggattgcg
     3121 tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc cccgccagtt
     3181 aaccaccaaa cagaccggcg atgcgtggga agcacaagcg cgtcgctggc tggaaggcaa
     3241 aggactgcgg tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg atctgataat
     3301 gcgtgaaggc ctgaccaccg tttttgtcga ggtacgctat cgccgctctg cgctttatgg
     3361 cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga ctgcccgctt
     3421 gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg atgtggtagc
     3481 cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact cataattaag
     3541 gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc attcaaactc
     3601 aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg acgctggttc
     3661 agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc gctgccaatg
     3721 cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc agcttacctg
     3781 ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat cgcttacatg
     3841 atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta ttgttagcca
     3901 tttccacccg tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc gttacgcgtg
     3961 atatgaccat tgtggcattg accggctatg acggcggcga acttgcaggt ttgttagggc
     4021 cacaggatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag gaaatgcata
     4081 tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct caccaggatg
     4141 attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc gcgctgctgt
     4201 tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc aaagccgcaa
     4261 ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg cgcgtgaaca
     4321 gcgcattgtc gaaagacgaa cagattaaga aagaaacccg tattaatgtc acggcctatc
     4381 agggcaaagt gctgctggtt gggcagtcac caaatgctga actttcggct cgcgccaaac
     4441 agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt cagggccagc
     4501 cgattggtct gggcgaagca tctaacgata cgtggatcac caccaaagtg cgttcgcagc
     4561 tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa aacggtgaag
     4621 tgttcctgat ggggctggta actgaacgtg aagcgaaagc ggcggcagat attgccagcc
     4681 gggtgagcgg cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag cagccccttg
     4741 taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca aaccgtaggt
     4801 cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaac aacagcgcca
     4861 gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat aaggctttcg
     4921 ccgggaacgc tttgcgcagc ataatcagtg acggcaaact cactgccggg agcgtcatca
     4981 acaatgccag cgccggagcg gttcccatac ctgccagcat catcgtttgt acaatcggaa
     5041 tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc accacccaca
     5101 tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcgt gcggcaccca
     5161 acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagtgtc catagcgccc
     5221 tgccccagcg gctaaaaaaa ccgctctgtg cttccggtat gtcaatttcg accggtgcct
     5281 gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc aacaccatca
     5341 ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac cccataaaca
     5401 ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca ccgcccatcg
     5461 atacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta cacatcatgc
     5521 ccggcagcga aaacagcgtt cccggcagcg tgccgcgaaa gcgcgattgc ccaagcgtac
     5581 gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc acccccagca
     5641 ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc gcttgccacg
     5701 gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg gctgcagtaa
     5761 acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg acaacgagaa
     5821 agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa gactgaccag
     5881 tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat
     5941 caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca
     6001 cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat
     6061 caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca
     6121 tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc
     6181 agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg
     6241 agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc agtcgccgcc
     6301 cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc tctttgctcc
     6361 ccgcttctcg ccgcgtggtg ccgagacaac aaaatacaat gtcgatgggt tcggtgacct
     6421 gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc atatcgccca
     6481 acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc aacatccgca
     6541 gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc atcttcgctc
     6601 ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca ccagtcttaa tagaccatga
     6661 ggtaattatc cccgattgtg gaaaattcgc ctcatccaat gcaacaacgc ggaggaagca
     6721 tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa tttacttcac
     6781 ccgcagacga gttccgtaaa gccggacacg aagtgattac tattgaaaaa caagcgggta
     6841 aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc atcgatgaag
     6901 tgacgccagc ggagtttgat gccctgttgc taccgggtgg tcattcaccg gattatctgc
     6961 gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc aaacctgttt
     7021 tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac
     7081 tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgtggaa ttttacgatc
     7141 aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac gatctgccag
     7201 cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca aatcattttt
     7261 ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc
     7321 ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg cgcgaggtcg
     7381 ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg aattaacgct
     7441 accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc actcatctgc
     7501 gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaaaaga
     7561 taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt gacaacatgt
     7621 tgttttgcca gccaacggct gatggcgatg agtgtttcca ttgctgttct cttttatact
     7681 gtgggcctga ccttaacatg cgatgcagac gatgaccccc tggtttcttt acttgatccg
     7741 taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc gctatcagca
     7801 gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt
     7861 ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg ttaagcaact
     7921 gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag aactgttgag
     7981 cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc aaccagaccg
     8041 gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct ttcagtgccc
     8101 ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg actgtacagc
     8161 gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct
     8221 tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac cgccaacggt
     8281 gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag
     8341 ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca
     8401 cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg cagcagggca
     8461 tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg
     8521 tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca
     8581 gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca
     8641 catacacccc cagctccgta tcgccttcaa tcaccagccg acgctggaag aagagcgtat
     8701 ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca ctaaaactca
     8761 catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag gtaaaccatt
     8821 gcaggtcaat atcacacaca tgaatactta accagcggcc ttcaagaaac tccagctcgc
     8881 catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta
     8941 gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac
     9001 gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc
     9061 catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc
     9121 accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc
     9181 cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt atggagctgc
     9241 tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg
     9301 tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg
     9361 agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatct
     9421 cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc gtggatatgg
     9481 cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gagtacgccg
     9541 ccgagcgtta cccgcatatt gaacgtcatg tgtcagtgca ggcttcggcg accaatgaag
     9601 aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt
     9661 tgtcgattca tcaggtgaaa cagctggcac gggttactcc tgtcccgctg gaagtgtttg
     9721 ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg
     9781 gtgagtcgcc caacactgtg ggtgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa
     9841 cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat caggacggcg
     9901 aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc
     9961 acgcgctgga agaaccaacc agtctcaata ccctggaact gctgccggag ttaatggctg
    10021 cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg
    10081 tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa aacttcgtac
    10141 cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa accacccttg
    10201 gcgcgtatca ccgtaaatgg cagtgagaag agcaatgaaa tattccttag ggccagtgct
    10261 gtggtactgg ccaaaagaga cgctggaaga tttttatcag caagccgccg ccagcagcgc
    10321 cgacgtgatt tatcttggtg aagcggtatg cagcaagcgc cgggcaacca aagttggcga
    10381 ctgtctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc tctccacgct
    10441 ggcgctggtg caggcatcct ctgaactggg cgaactgaaa cgctatgttg agaacggtga
    10501 gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc
    10561 gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa tattgctcaa
    10621 acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc tggtgaatct
    10681 gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag tcctgagcta
    10741 cggtcatcta ccgctggcct actccgcccg ctgctttacc gcgcgttcgg aagaccgccc
    10801 gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg tgctgtcgca
    10861 ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct acgtttacaa
    10921 cctcggtaac gagctggcat ccatgcaggg attggttgat gtagtccgcc tgtcaccgca
    10981 gggcactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg gcgctgtgcc
    11041 actgccgttg acggcaaaca gcgactgtaa cggctactgg cggcggctgg caggactgga
    11101 gctgcaagcc taagtaaata gctcactttg ttaacaactt taactattct ttaatgcagt
    11161 attaaagatt aatcggtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg
    11221 ctactcgatc tggcccccat acccgaaggc tcttcagcgc gggaagcgtt ctcccactct
    11281 ctcgatctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcagaacac
    11341 cacaatatga ccggcattgc cagtgctgca acatcggtat tgattggcta tctggcggcg
    11401 aataccacca cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca ctcaccgttg
    11461 gtcattgccg aacagttcgg cacactcaat acgctctatc cggggcgaat cgatttgggg
    11521 ctgggtcgtg ctccgggtag tgaccaacgg acaatgatgg cgctacgtcg tcatatgagc
    11581 ggcgatattg ataatttccc ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc
    11641 gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg
    11701 ttaggctcca gcctttacag tgcacaactg gcggcgcagc ttggtctgcc atttgcgttt
    11761 gcctcacact tcgcgccgga tatgttgttc caggcgctgc atctttatcg cagtaacttc
    11821 aaaccgtcgg ctcggctgga aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc
    11881 gacagcaacc gcgacgctga atttctgttt acctcaatgc agcaagcctt tgtgaagctg
    11941 cgccgtggcg aaaccgggca actgccgccg ccgattcaaa atatggatca gttctggtca
    12001 ccgtctgagc agtatgacgt gcagcaggcg ctgagtatgt cgttggtagg cgataaagcg
    12061 aaagtgcgtc atggtttgca gtcgatcctg cgcgaaaccg acgctgatga gattatggtt
    12121 aacgggcaga ttttcgacca ccaggcgcgc ctgcattcgt ttgagctggc gatggatgtt
    12181 aaggaagagt tgttgggata gtgtgtctta acgcgggaag ccttatccga gctggcaacg
    12241 ctgtcctacg taggcctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt
    12301 cggataaggc accgctgatt actgatacac cggcagtaaa ttaaagctcg ataaaatatg
    12361 caccagtgcg ttgccgacgc caaacaccag aatcagcgca atcatcggct tgccacccca
    12421 gacgcggaat ttcgggctgc caaagcgttt acgcgatgcg cgggccagca gtgccggaac
    12481 aattgccgcc cagatggtag ccgctaaacc agcataacca atggcataca ggaatccgtt
    12541 cgggaacagc agaccaccca caacaggcgg ggcaaaggtc agcaatgccg ttttcaagcg
    12601 gcccacagcc gagtcgtcga aaccaaacag atctgccaga tagtcaaaca aacccagcgt
    12661 tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca gaaggtccag
    12721 gctacggctg ttcagcacgc cgcttaatgc ttgtaccagc acatcaatat taccgccctt
    12781 ctcggcgatg ccgataaact ccggacgagg gatgttgccc attgtcgcca gcaaccagat
    12841 ggtatacagc gccagtgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg
    12901 atctttgccg taatacttca tcaggcttgg cacgttaccg tgataaccaa acgacgccaa
    12961 gcagaacggc agggtcatca gcaaatacgg cgcataagat gcattgcttt cggcaacgtt
    13021 gaacaatgtc gcaggctgca catgccccag caggctaccg aaggtgagga agaaggtaat
    13081 gactttcgcc cccagcacaa tcgccgtcat gcggctgacg gctttagtac tcaaccacac
    13141 cacaaacgct accagcagtg caaaaccaaa acccgccgcc cgtgccggga cgtttagcga
    13201 catctctgcg aaggtgtgat gcagaatcga accactggca gaaatatagg cataggtcag
    13261 gatatagagc acaaaggcaa tggaaatgcc gttgaccacg ttccagcctt tgcccagcaa
    13321 atctttggtg atggtgtcaa aactcgaacc gattcggtaa ttcaggttag cttccagaat
    13381 catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgctgcca ttgaccagaa
    13441 aaaccacgcc ccggacatga ccactggcag agaaaacatc cctgcgccaa taatggtgcc
    13501 gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc
    13561 catgagggct tctctccagt gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac
    13621 cagtacatga gtacaaaaga cagaaaaaaa gccccgatgg ttaaaatcgg ggctgtatat
    13681 attattttac agattgtgtt cgctgttcag cgatgattac gcatcaccac cgaaacgacg
    13741 acgaccggta gagtcatcac gacgcggtgc gcggccttca cgacgttcgc cgctaaaacg
    13801 acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacgac cgccttcacg
    13861 acgctcgcca ccgaaaccac gaccaccgcc acgaagctca ccgccagtat gcggctgtgc
    13921 atcgcccagt aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg
    13981 cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt
    14041 gatgttaccg atgtaacggc tgctgatgtc gccttcgtta gcaatcgcgc caacgatatg
    14101 acgaacttca acaccatcat cgcggcccac ttcaatgcgg tacagctgca tatcgccaac
    14161 atcacgacgt tcacgacgcg gacgatcttc acggtcgcca cgcgggccac ggtcgttacg
    14221 atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc
    14281 tggcggaaca atcagagtac gttcaccctg tgccattttc agcagtgccg cagccagagt
    14341 ttcgagatcc agctcttcac cttctgcagt cggctgaatt ttgctcagca gtgcgcggta
    14401 ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg
    14461 gcgtttgccc agcagttctg cgttcggcag ttctacttcc ggaatagtca gcttcatagt
    14521 acgttcaatg ttgcgcagca gacgacgctc gcggttctca acgaacagca gcgcgcggcc
    14581 agcacgaccc gcacgaccgg tacgaccgat acggtgaacg taagactcag aatccatcgg
    14641 gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc aggccacggg ccgcaacgtc
    14701 ggtcgcaatc aggatgtcca gacgaccgtc tttaaggcgt tccagtgtct gctcacgcag
    14761 cgcctggttc atgtcaccgt tcagcgcggc gctattgtag ccgttacgct caagagcttc
    14821 agccacttcc agagtcgcgt ttttggtacg aacgaagata atcgccgcat caaaatcttc
    14881 cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccacacag tccagtagct
    14941 ctggctgatg tcaggacggg tagtcacgct ggactgaata cgcacttcct gcggctcttt
    15001 cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt
    15061 ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg
    15121 cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc
    15181 acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg
    15241 cagggcgcgt aattgcacgt cataacgctg gccgccgtac agggcaacca catttacgcc
    15301 gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt
    15361 cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct gcagcagagg
    15421 taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc
    15481 attcagcaga tgtggaatac actctgcctg aattggagat ggtttttcgt aacccagatc
    15541 gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc
    15601 agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa
    15661 attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac
    15721 aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac
    15781 gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat
    15841 tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg
    15901 aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc taaatagaag
    15961 ttggtttcac tgagatgctc agcgagcgag gtgttatcgg ttgcgtccgc cttgagcctt
    16021 tccattaacg tgtgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac
    16081 tgttccttat ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg
    16141 agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga
    16201 taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcaatc
    16261 ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct
    16321 tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaatact
    16381 tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc
    16441 agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg
    16501 gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa
    16561 gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct
    16621 gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc
    16681 cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt
    16741 taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc
    16801 ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg
    16861 atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc catctgcagg
    16921 taatcggtca ctttctcaac gcgtttgtca gcgatttgag agatgtgtac cagaccttct
    16981 ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga tacgggtcac tttaccagtg
    17041 tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat agcatgtttc
    17101 gctttctcgc cgtcggtcgc tgcaatcttc acagtaccgt catcttcgat ttcgatggtg
    17161 gtgccagttt cttcggtcag ggcacggata acagagccgc ctttaccgat aacgtctttg
    17221 atcttgtccg ggttgatctt gatggtatgg atacgcggtg cgaactcaga gatatcgcca
    17281 cgcggcgcgt tgatcgcctg ttccattacg cccaggatat gcagacgcgc acctttagcc
    17341 tggttcagcg caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc
    17401 aatgcagaga taccctcgcg ggaacccgca actttgaagt ccatatcgcc caggtgatct
    17461 tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt
    17521 gcgatacccg caacggcagc tttgatcggc acacctgcgt ccatcagcgc cagagacgcg
    17581 ccgcacacgg aagccataga agaggaaccg ttggattcag tgatttcaga cacaacacgt
    17641 acggtgtacg ggaatttgtc catatccggc atgactgcca gcacgccgcg cttcgccaga
    17701 cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacggag
    17761 tacggaggga agttgtagtg gaacaggaag gtatcggtac gttcgcccat cagttcatca
    17821 agaacctgcg cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg cgtttcacca
    17881 cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac atccagacca
    17941 cggatcatat ctttttcacg accgtcgata cgcggttcgc ctgccagtac gcggctacga
    18001 acaacgtttt tctcgatcgc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg
    18061 tcttcagcaa gcagcgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc
    18121 tcttgtttgt cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag tgcagcaacg
    18181 cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg tttaccggct
    18241 tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg accgaacact
    18301 actgcgccca gcatctggtc ttcgctcagc agttcagctt cagattcaac catcagtaca
    18361 gcggcttcag taccggcaac aaccagatcc agtttgctct ctttcaactc gtcctgagtc
    18421 gggttcagta cgtactggtc attgatgtaa cctacgcgag cagcaccaat cggaccattg
    18481 aacggaatac cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg
    18541 ttaacttgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct
    18601 tccgggaaca gcgggcgaat cgggcggtca atcagacgcg cgatcagggt ttcgccttcg
    18661 cttgggcggc cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc
    18721 tcctgatagt taacggtcag tgggaagaag tcctgacctg gtttggcttt tttctggcca
    18781 acaacggtaa caaataccgc ggtgtcatcc atgctaacca taacagcggc agtagcctga
    18841 cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg
    18901 atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac ggcactggtg
    18961 ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt
    19021 gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa
    19081 tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggcccct
    19141 ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag ctgggtgtaa
    19201 cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga aaccatgcgc
    19261 agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc ctgcaggtgg
    19321 ttgatctgtg cagtcagcag tgctacctga acttcagtag aaccggtgtc gtttgcgtca
    19381 cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat tttaaaactc
    19441 caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt gtacgttacg
    19501 caaagtgtta aacaatttac gcgacgttaa gcggcagtat tctactcgta gcgacctgtt
    19561 atcgcaagac ggttaacatt acgccgggta ttcaaccacc aggcgacgag gcgcaacgcg
    19621 gccttcatcg tcaatttcac ccataccgat aaatttgccg ttctcacctt ctgtaacgcg
    19681 aaccagtcct tccagcggcg caccggatgt acgaactggg ttaccatttt tgaagtaaac
    19741 agaagacgtt aacggaagat tcaccaccgg gtagtccgaa gctggactgt ccattggcat
    19801 cagtaatgga tcaagtaact ccgcagccgg aatatcctgc tgttcggctt gttcgaccag
    19861 ttcacgcaga tgttccaggg tcacaatccg ttcaaccgga tatttactta ccgccagacg
    19921 acgcaggtaa ataacatgcg cgccacaacc gagtttttca cccaggtcat caatgatggt
    19981 gcggatataa gtgccttttg agcagtgaat ttccagctcc agctcattgc cttcgtggcg
    20041 aataaacagc aattcataaa cggtaatcgg gcgcgcttca cgcggtactt caatgccctg
    20101 acgcgcatat tcgtacagtt ttttaccctg atatttcagt gctgaataca tcgaagggat
    20161 ctgttcgata tcgccacgga aagtatccag cgccgccgcc agttgctctg cgctaaaggt
    20221 taccggacgt tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac gctgtccaag
    20281 acgcgcaatg acccgatagc gtttgtcgga gtccagcaga tactgggaaa acttcgtcgc
    20341 ttccccgagg caaattggca acatgccagt tgccagcggg tccagcgcac cggtatgccc
    20401 ggcacggttg gcgttataaa tccgtttcac tttttgcagc gcatcattgc tggacatacc
    20461 ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac gaggacgact
    20521 cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg tttgaccacg
    20581 ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta gaagaaggtc
    20641 agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat gaaaccagaa
    20701 gcttcttgca acgctttgat gcccgcttta accgcgtctt catctttgtc gttgaggaac
    20761 gtcacatata ctttggcata cgccaggtcg cgagacattt cgacaccgga aacggtggtc
    20821 atcatgccca ggcgaggatc tttaatttca cgctgcagga tgagagcgat ctctttttgc
    20881 atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa ttctcctgac
    20941 aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag gtggaagggc tgttcacgtt
    21001 ggcctgataa gacgcgccag cgtcacatca ggcaatccat gccggatgcg gcgtaaacgc
    21061 cttatcccgc atggaaccct aaaaacctta agcgatggta cgttggatct cgatgatttc
    21121 gaatacttcg atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc cgataccaca
    21181 ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg actccagctc
    21241 gccttcgtag ataaccacgt tgtcacgcag aacgcggatc gggttgtgac gtttaaccac
    21301 accttcggta accatacagc ctgcgatggc accaaatttc ggcgatttga acacgtcacg
    21361 aacttccgcc agaccgataa tctgctgttt cagttccgga gacagcatac cgctcatcgc
    21421 cgctttcact tcgtcaatca ggttatagat gacggagtag taacgcagat ccaggctttc
    21481 cgcttcaatc actttacgtg cagaggcatc agcacgtacg ttgaagccaa ccaggatggc
    21541 gttggacgcc gcagccaggg tggcgtcggt ttcggtgata ccacctacgc cagaaccgat
    21601 gatcttcact ttaacttcgt cagtagacag tttcagcaag gagtcggaga tcgcttccac
    21661 agaaccctgt acgtctgcct tcaggacgat gttcacttcg tgaacttcgc cttcggtcat
    21721 gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa cttcgcggaa
    21781 tttaccctga cgatacagtg caacttcacg cgctttcttc tcgtcacgta caacggtaac
    21841 ttcatcaccc gcagccggta cgccggacag gccgaggatt tccaccggaa tggacggacc
    21901 cgcttccagc acttcctgac ccagttcgtt acgcatcgca cgaacacgac cgtattcgaa
    21961 gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa cggtagcaac
    22021 cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca tacctttacg
    22081 taccgctttc agctccagaa cttccgcctg cagcaggata gcgtccagca gttcatcgat
    22141 accagtaccc gctttcgcag acacatgtac gaactggctt tcaccgcccc actcttccgg
    22201 caggatgccg tactgggaga gttcgttctt aacgcgatcc ggatcggctt ctggtttatc
    22261 gatcttgttc actgcaacga ccaccggaac ctgcgccgct ttcgcgtgct ggattgcttc
    22321 gatcgtctgc ggcattacgc cgtcgtcggc agcaacaacc aggactacga tgtccgttgc
    22381 ctgcgcacca cgagcacgca ttgaagtaaa cgcggcgtgc cccggggtgt ccaggaaggt
    22441 gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgagtaa tgccgcccgc
    22501 ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt taccgtggtc
    22561 aacgtgaccc atgatggtca caaccggcgc gcgcggttca gccgcagcac ccgtgtcacg
    22621 gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc aggataactt tatggcccat
    22681 ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg ttgccattgc
    22741 gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct tgttcgccag
    22801 ttcgccaacg gtgatagttt cgccgatcac aacgtcacgg ttaacggcct gagcaggctt
    22861 ctggaagcct tgctgcagcg aagaaccttt acgttttccg cctttaccgc cacgtactgc
    22921 tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt tcttcggacg
    22981 cgctgctttc gcgttacgac cacggccacg gccgccttcg acttcacgat cgctttcgtc
    23041 ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa tcgctggaat cttcagtcgg
    23101 ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt cagcaacgcg
    23161 acgtgcttct tcttcgagtt tacgacgcgc ttcttcttca gctttacgct tgagctctgc
    23221 agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat cgtcttgttg
    23281 attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag cttcacgctt
    23341 agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcgcgtttag ccgattcttc
    23401 tgcctcacga cgggcttgct cttccgctgc aaggcgttca gcctcttgcg gatcgcgttt
    23461 cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc caccggtacc
    23521 aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg ggcctgaatt
    23581 tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag agtcgtcagc
    23641 agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg aggtctgtcg
    23701 ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg ctacagttta
    23761 ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg ctccggcttt
    23821 ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg cgagatcttc
    23881 cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta ccccttcaag
    23941 gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg cctgtgcaat
    24001 ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct catcaaggcc
    24061 ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg tcgagaaacc
    24121 ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg tgaaggtgtc
    24181 gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg tcattacgtt
    24241 gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac ggccaatcgc
    24301 ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt catccaccac
    24361 gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg ggttatcatc
    24421 ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct gaacacgcgc
    24481 gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg ttttcaccgc
    24541 gattttcgca cgagaacccg ggtcgcgagc cgctgcttta atttcaatca cttcttcgcc
    24601 gatttccggc acttcaatac ggaacagttc gatcagcatt tccggcttgg aacgagtgac
    24661 gaacagttgc gcgccacgcg cttccgggcg aacggaataa agcacgccac gaacgcggtc
    24721 gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg cttcagcatt
    24781 gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc cggtgatgat
    24841 ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt cacgcacttt
    24901 ctgcacgata acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa cagactcaat
    24961 ctgatcttca acgtaatcgc ccaagttcag gctttcatct tcataacgtg cggcttcaag
    25021 ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac ggaaggtgtc
    25081 aaaatcaccg cttttgcgat cgatctgtac acggacgtcg atctcttgct catatttttt
    25141 ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc gaggtagcgc
    25201 cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg cgggcttttc
    25261 acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat tactcagcgc
    25321 gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta ccgctttgat
    25381 aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag tcacctcttc
    25441 tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac ccggtgagga
    25501 gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag cacttacctg
    25561 gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa tatagatgcg
    25621 cagtgtggat gtgcgaccgc gaataaattc gatgcctacc agttcaaaac ccagggcctc
    25681 aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc ccacccccaa
    25741 gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa aaaccccgat
    25801 aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga gcactttcca
    25861 gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat ttgaaaaagg
    25921 actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg ggttatgagc
    25981 ccgacgagct accagactgc tccaccccgc gcctgaaacg tggcaaattc tactcgtttt
    26041 gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt aaaatctgca
    26101 ggcattatag tgatccatgc cacattttgt caacgtttat tgctaatcac gtgaatgaat
    26161 atccagttca ctttcatttg ttgaatactt ttgccttccc ctgctctccc ttaagcgcat
    26221 tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat taaatgaaaa
    26281 ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc atcagttaat
    26341 taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg tcaacgtatt
    26401 ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat gcgacaaaag
    26461 ggagcggttc cttatgcata tactgcaaac ctaggccagc cagacgaaga ggattatgat
    26521 gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat cgactgccgc
    26581 aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca taacaccacc
    26641 ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg taccatgctg
    26701 gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac ctataaagga
    26761 aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact gcagatttac
    26821 aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga gatgtctgaa
    26881 tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta ctccacagac
    26941 tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa ctccagcgtc
    27001 aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa gatcccggca
    27061 gaagaagtca cagtacgctt tgagcaaggt catccggttg cgctgaacgg taaaaccttt
    27121 agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca cggcctgggc
    27181 atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat ttacgaagct
    27241 ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat tcacaacgaa
    27301 gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct gtaccagggg
    27361 cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg ggttgccagc
    27421 cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc aatcctgaat
    27481 accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa aggcgactcg
    27541 gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga tatcactgat
    27601 acccgagaga aactttttgg ttatgccaaa actggcctgc tttcctcctc tgccacttca
    27661 ggcctgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat caaccctgcc
    27721 cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc atcacataat
    27781 cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt gcaatcactt
    27841 cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta ataaggtaat
    27901 tattgtaagc tgtcgaaaag atattgtttt ccgttgtgcg atccacgcca tccccaagaa
    27961 cggggctata acagataaac atcgggacat gatatgcctg ctggctagcc tccctgcctc
    28021 catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg gcaaaataca
    28081 tgaccgaggc gcggcgatct tttaataatt caaaaaactg acctagcaaa ctatctgtgt
    28141 aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac acgcgctgga
    28201 ttgcggatag gcgctacaag ccggttcatg gcttccattt aaatgaagaa caatcagttt
    28261 cttttgctgc gtattttgct gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa
    28321 tcctctgaca taaactgttt ccatggcgcg catggcgata ctggtaactg ctgtaccatt
    28381 ctgccgaaaa gcggattgcg agcttagcca gaaagtctga aatcctgctt gattagccat
    28441 attaataatg ttgtcggggt aattatgaat gtcatgactc aaaacagaat cagcagttaa
    28501 agaaagggga accgacagcg cagtgtaagg tgcgccgctt attgcttgat taaacagttt
    28561 gatctgtttt ctttgtgctt caacttgcgg tgtcgtagag cgtgtatatc catacaaaga
    28621 catattgtcg acacgtacag actcccccac aatcaacacg taggtatcaa tacttgtatc
    28681 cctgactgat aattgaaaat acggcaccgt gtttgcaatt gagagtaatc tttgatgctc
    28741 tttcgctgct aaagcaaaat agttgagatt gaaaaacggc gtataggtag caaatcacga
    28801 cgctagtata tatggactga acgctttttt attttttgca tctttataag caaattgaca
    28861 agcggaaaat aaactgcccg aaatgacaat cagcaataat attcctgtca ctttttttgt
    28921 cggcaaggaa acatcatatt ttattattac tgccaaaaaa aaaagggata aaaaagcaaa
    28981 agcacataga taagggatat acatccccag cattttcaca acttcatccg gatcactctg
    29041 cagcacacta atcgcaaatc catcattgaa agttgtacca aaggtacacc atgaatagag
    29101 gctaatactc atgtctgccg cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag
    29161 cggaagggat gatatacgtt taacaaccag gatggtcagg acgaagaaca gcaatcgctg
    29221 aaccatatgc gctccggaag aggcgactaa gttcgtaata ccaaaaacaa taacacaaag
    29281 atacaacaac caatgagatt taaaatcttc tagcaaattt gttgaatact gtcatccaga
    29341 aatccaagtt cacaaccaat ataaacaatt atcaacaatg ttcaatttta tatcagacat
    29401 gtttttcaac tctgaaccat atcagaccga aacactgtta gttttttctt tttaaatgat
    29461 gcgaccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa acattacgca
    29521 gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag aagtaaacat
    29581 atccgtattc gtaccggaac tggagttgac acaataaagt gccaattatg tcagcagtag
    29641 ggaaaagttt ataactaagg cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa
    29701 agcaactgga cgagatacag atacctgaca accattcatc agaccaggac caaaacgaaa
    29761 agagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga cttgaacccg
    29821 taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca ccacctcggc
    29881 acggatacta ctttttagtt cgggatatcg ctggtcggct tagccggagc agctggctga
    29941 gtttgttcgg ttttcgccgg tgcactcaga ttttcccatt cgctacctct attggttttg
    30001 ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc cagcagcgcc
    30061 gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc ggaagcgcct
    30121 gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat cagaccaaca
    30181 aggccaattg ccacaataag gaaaactact aaaagagctt cgtacataat caacctgttc
    30241 cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt taatctttcc
    30301 cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc aattttatcg
    30361 cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga
    30421 caatcgccgc ctttttagcc agttatctaa cgctttaaac ggcttttact gcatcggcga
    30481 tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta
    30541 acggttcggt gccggattta cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa
    30601 cctctgcggt cacggcttta actgactcat gctcaagcgg atcgccgcta cctgcggtgt
    30661 aacgtacgtt aaccagaatc tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc
    30721 tcatatggtt acgtgccatc gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg
    30781 tagtggtttt atccagcagg atcacatgac cggaattctc tgcaccgata cgccagcctt
    30841 tctcctgcat tttttccagt acgtagcggt cacccacttt cgcgcgcgca aatggaattc
    30901 ctaactgttt cagcgccagt tcaagcccca tgttgctcat caatgtaccc acagcgccac
    30961 cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc tgatcgccat
    31021 cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa
    31081 taccgagatc cgctttttca gccagcacac gagcctggag cacgcgaacg tcggtagccc
    31141 ccacttcggc attgatgttt acaccgtttg gctcacaacc gatagcgata acgttcgccc
    31201 ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca caatccacca
    31261 caatcttcag ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat
    31321 agcgacccgc ggcatcaacg atacggctgg ctttacccag ttctgccgaa tcaacgcagc
    31381 tgatctcctt ttccatttcc gcttcgatgg cctcttctac cgcatccggc agtttagtgc
    31441 cgtcgataga gaagaattta atcccattat cgtagaacgg gttatgcgat gcagatatca
    31501 caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt gttggcatcg
    31561 ggccagtgaa gagtgcggaa aggcccgctg ccgccagacc cgcttccagt gctgactcca
    31621 gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag ccgtggcgcg
    31681 ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggtgtg atcggcgcat
    31741 cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc atagcgtttg
    31801 ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt ttctttgacg
    31861 tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca ggccagacta
    31921 ccgctcaggc gctcggacgg ccccacgttc agcagctgcc caatcatcga ttttcgtgac
    31981 atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa
    32041 tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa tttctctttt
    32101 gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt cacttctgca
    32161 aagacatcgt catacttcgg agcttcctgc atggtttttg gatttccctg catatgcatc
    32221 agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc ggaaagggag
    32281 cggatatcat taataatgtg agcgccaact ttcgctgact cacggatgac ttctggtttg
    32341 gatgtatcga ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac cacaggaata
    32401 acacgttgca actcttcttc aacgctaact tccgccgccc ctgggcgcgt ggactcgcca
    32461 ccaacgtcaa tgatcgtcgc gccagcattg atcatcagat tcgcatgttt caccgcatct
    32521 atcagcgagt tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt gaggatcccc
    32581 attacgtgag gatggctaag gtccagtgaa gtaccctggg caaagagttt catgatgtta
    32641 tccctggtat gaattgataa gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa
    32701 tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg gtgttacccg
    32761 ggttcggcgt acgcggttca tcaaccggac gaggagcctt tggactacca ttgtcgccag
    32821 aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca
    32881 tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca tctttcatcg
    32941 catgcagaat atccatattg tcggtcagaa gctgacgcgc acgattatag ttacgctcaa
    33001 tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata tgtttcgctt
    33061 tcgctacgct acggccgagg aatacttcac cttcttcttc cgcgtacagc agtggaccca
    33121 atttctcaga gaagccccac tgagtcacca tgttacgtgc caggttggtc gcaactttaa
    33181 tatcgttgga cgcaccggta gaaacatgtt ccggcccgta gatgatctct tctgccagac
    33241 gaccaccgta cagcgtagaa atctggcttt ccagtttctg acggctggcg ctgattgcgt
    33301 cgccctcagg caagaagaaa gtcacaccca gcgcacgacc gcgtgggata atcgtcactt
    33361 tatgcaccgg atcgtgttcc ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt
    33421 aagccgttga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc gcacccatca
    33481 tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag
    33541 cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag aaaccaggag
    33601 taccacgggc aatgattgcc gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa
    33661 ctttcaggat ctgctcacga ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga
    33721 aacggccagg acgcaacagg gccgggtcga gaacgtccgg acggttagtc gcggcgataa
    33781 cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg ttcagagtct
    33841 gttcacgttc atcgtgacca ccgcccagac cagcgccacg ctggcggcct acggcgtcga
    33901 tttcatcgat aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa
    33961 cacgggatgc acccacaccg acgaacattt ctacgaagtc agaaccggag atagtaaaga
    34021 acggaacttt cgcttcgcct gcaatcgctt tcgccagcaa cgttttaccg gtacccggag
    34081 gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag cggctcggct
    34141 cgcgcagata ctctaccagt tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt
    34201 cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg
    34261 acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc cagacaccaa
    34321 tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt ggttcttcag
    34381 gcggttcacc gacaaccttg acgttcttgg tcaacaggtt atccagtaat ttcggatcct
    34441 gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg atttcacgtc
    34501 cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag gtagagtaat
    34561 ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac atcagcacaa
    34621 cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga ttaacctctt
    34681 attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt tcaaactttc
    34741 gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact tctcgcgaac
    34801 gtgcacgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag cgaatttccc
    34861 ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg ccacctggcg
    34921 ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca cgggggatat
    34981 ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc tggactttgc
    35041 tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa tcgccctgaa
    35101 gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg atgcggcctt
    35161 tgccgccaat ttgggtgacc acatattgtg accaaccacc cggagcagca ccaaggtcga
    35221 caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca agtttaaacc
    35281 aggcacggga acgtaatccc tttttctgtg cctgttgaac atatttatcg ctaaagtgtt
    35341 cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa ctttcccatg
    35401 gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggt gatatatggg
    35461 agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac gatgaatctg
    35521 agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc agttgttctg
    35581 cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc gttagagcac
    35641 catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg
    35701 gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg
    35761 ctttatcgcc caactaaaaa acgtaaaatc tcgctgccac gctaagatta tcctaaagtt
    35821 acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa aatgccacgc
    35881 tctgttcgtt gataaaaggc cgcagagcgg cctttttcct ttctttacaa tacatcaaca
    35941 tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa ttctacttcg
    36001 ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag gccacgagca
    36061 ataggcgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc gccaacgatg
    36121 cgataagtct gttcttcgtc agaatccaga ttcagcaccg ttacggtagc accaaaaata
    36181 acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgtttga cagcttggct
    36241 tcgatgtctt tgatacggcc ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg
    36301 gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat gatttcagga
    36361 cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc
    36421 atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca agtcttcacc
    36481 caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag caaaaaaata
    36541 ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatggtc attttaccct
    36601 gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta tgacggctcg
    36661 attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg attgaccagc
    36721 tgtatagcgt tcagtgttca ggccgcgaat gttgatgagt acgttactca actccccgca
    36781 ggtgccaacc ttgccctgat ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac
    36841 cacagtcagc agatggcgct gcccgccagt acccagaaag tgattactgc gctggcggcg
    36901 ttgattcaac tcggccccga ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg
    36961 gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa
    37021 cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat
    37081 ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc cggctggcca
    37141 tggaatgaca tgacacaatg ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac
    37201 tgtttctccg tctcgctcta cagtgcccca aagcctggtg atatggcttt tatacgcgtg
    37261 gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg tggttctgcc
    37321 gaagcgcaat actgcgaact ggatgtggtg ccaggcgacc tgaaccgctt tacgctgacg
    37381 ggatgcctgc cacaacgttc tgagccgctc ccgttggctt ttggcgtgca ggatggagcc
    37441 agctatgccg gtgcaattct gaaagatgag ttaaaacagg cgggtatcac ctggagcgga
    37501 acactgctgc gccagactca ggttaacgaa cctggaacgg tagttgccag taaacagtcg
    37561 gccccgctgc acgatctgct taagattatg ctgaaaaagt cggacaacat gatcgccgat
    37621 acggttttcc gcatgatcgg ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg
    37681 tcggacgccg tgcgtcagat cctgcgccag caagccggtg tcgatattgg taacaccatt
    37741 attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac catgatgcag
    37801 gtgctgcaat acattgccca acacgacaat gaacttaact ttatctccat gctgccgctt
    37861 gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc
    37921 aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg attcattacc
    37981 acagcgagcg ggcaacgaat ggcatttgta caatatcttt ctggctatgc agtaaaaccc
    38041 gcggatcagc gtaatcgccg tattccgtta gtgcgttttg aaagccgttt gtacaaagat
    38101 atttatcaga acaattagtc aaaaagaaac cccggcgcat ggccggggtt tcagattatt
    38161 gacaaagtgc gctttgttta tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat
    38221 cgtgcaaatt caatatattg caatcatctt gtaggcctga taagtgtagc gcatcaggct
    38281 tatttggcat tggtcattag tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg
    38341 tcttcgtccc agtcgtcatc ccagtcttca tcatcctctt cagcaatctc ttcaagctgc
    38401 tgacggtgat aatcatccca catgaattcg actttctctg gctgtttcgc ttcttcagcc
    38461 tgcacgaccg ggttttcaat gataaaggtc atcacatccc agcagagatc tttcacgccc
    38521 agtccgctcg ccgcagagat cagataatat ttatcttccc agcccagcgc ttcagcgatc
    38581 gctttcgctt tctcttcggc ttctgcctta tccagcagat cgatcttgtt gaagactaac
    38641 caacgcggtt tcgccgccag atcctggctg tatttttcca gctcgctgat aataatacgc
    38701 gcgttttcaa ccggatcggt gccgtcaatc ggatcgatat cgatgaggtg caacaggacg
    38761 cggcaacgtt ccaggtgctt caggaagcga atgcccagac ctgcgccttc cgcagcgcct
    38821 tcaatcagtc ctggaatatc ggcaacaacg aagctctttt cgttgtccat tcgtaccaca
    38881 cccagacttg gcaccagagt ggtaaacgga taatccgcca ctttcggttt agccgccgat
    38941 accgcacgaa taaaggtcga tttacccgcg tttggcatcc ccaacatacc gacgtcagcc
    39001 agcagcatca gctccagcag cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc
    39061 cgtggtgtac ggttaactga cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc
    39121 ttagcaacca gcagacgctg accgtgttta gtcatatcac ccatggtttc accagtaccc
    39181 tggtcgatta cacgcgtacc taccggcact ttaatcgtca cgtctttacc gcgcttaccg
    39241 gtacaatcgc ggcttgcgcc gttctgaccg cgctctgcac ggaaagattt ttcaaaacga
    39301 taatcgataa gcgtgttcag gttctcgtcg gcttccatcc atacgtcacc accatcaccg
    39361 ccgtcgccgc catccgggcc gcctttcgga atatactttt cgcggcggaa gctcacgcaa
    39421 ccattaccgc catcacctgc aacgaccaga atcgatgctt catcaacaaa cttcatttta
    39481 ttctccgtaa atcattcgcc tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat
    39541 ccgccccaaa tacgatgacc aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca
    39601 ccgagataac ctaaaaggtt taacatcggt ctggcgaaga aatcgggcca ggccagtgat
    39661 aaaagatctg aaaaaaacag cgtaaacagt ggggtgagcg tgatgatcgc gctcacctga
    39721 gctgcctgcc agcgagccat cgcttccgcc aaggcgccat atcctaccaa ggtattcagt
    39781 ccgcaaaaaa ttaaacatgc gagctgccag tggctaagct gcgctatcac tccaggcttt
    39841 gccagaggga agagcgcgat tgtacataaa gtgtacagta aaaacaggat ctgcggtgag
    39901 gccagccgac gcaataaaac cttttgcgcc acgccataac tcacccaaac cgtcgccgca
    39961 ccgaccccaa agataactcc ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga
    40021 ctggtgttaa aaaacatcac cagaccgctc aggagcatca atgcccctac aacctgagtg
    40081 ctgcgcattt tctctttcag gataaatacg ctggcaacca tcatgccaac tggcgagagt
    40141 tgcccaatca cctgcgaagc ggtcggactc aggtattgca aggatgagct gaacaggatg
    40201 aagttcccaa acagcccggc ggtcgccact gccaacaaaa tcaaccagcg tggcttacga
    40261 aacacgcgta atggcggcaa cctcttcttc accgcaagaa tggcaccaag gccaatactc
    40321 gccatcaaga aacggtaaaa cacgattgtc ggaggttcca tcacctccag cacctgcttc
    40381 attgcgattg gcaacgcccc ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg
    40441 cctgcctgct gcttcatacc gtattccctg caaagcgcat ttgcggatta acgtcgcacc
    40501 atgcgcattt ttaccggtta tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca
    40561 tccgttaccg ggacgcgaaa aacttattca gcttcgatgc tgataaattt acggtttttc
    40621 gggcctttaa cttcgaattt cactttaccg tctgctttag caaacagagt gtggtcacga
    40681 ccgcaaccta cgttagcgcc agcgtggaat ttggtaccac gttgacgaac gatgatgcta
    40741 cccgccagaa cggattcgcc accgaaacgc ttaacgccca ggcgtttagc ttctgaatcg
    40801 cgaccgttac gtgtggagcc gccagccttt ttatgtgcca tttgaaatct ctcctcaggt
    40861 cttaggcgct gatgccagta attttcacat cagtgaacca ctgacgatgg ccctgctgct
    40921 tacgatagtg tttacgacga cgaaacttaa cgattttaac tttctcgcca cgaccgtgag
    40981 caacaacttc agctttgatt acgccgccat caacgaaagg aacgccgatt ttgacttctt
    41041 caccgtttgc gatcatcagc acttcagcga actcaacagt ttcgccagtt gcgatgtcca
    41101 gcttttccag gcgaacggtc tgaccttcgc ttactcggtg ttgtttacca ccactttgga
    41161 aaaccgcgta catataaaac tccgcttccg cgcacacctt ttcaatgatt caaagtgcgc
    41221 tataaatatt cacaataggg cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta
    41281 cagtcaatac acgaagaaaa aaaacacaac ttgtacggta acgtttatct gtgccatttt
    41341 ttcagtacaa ttaccctata ttcctaacca taaaccctaa gttgcctttg ttcacagtaa
    41401 ggtaatcggg gcgaaaagcc cggcttttgc gatgaattta gaaaaaatca atgagttaac
    41461 cgcgcaagat atggcgggtg ttaatgcggc aatccttgag cagcttaatt ccgacgtcca
    41521 actgatcaat cagttaggct attacatcgt cagcggcggc ggtaaacgta ttcgtccgat
    41581 gattgctgta ctggctgcac gagctgttgg ctatgaggga aatgcgcatg tcaccattgc
    41641 tgccctgatc gagtttatcc acacggcgac tctgctacac gacgacgttg tggatgaatc
    41701 agatatgcgc aggggtaaag ctaccgccaa cgccgcattt ggcaatgccg ccagcgtgct
    41761 ggtaggcgat tttatttata cccgcgcgtt ccagatgatg accagcctcg gttcactcaa
    41821 agtgctggaa gtcatgtcag aagctgtaaa cgtcatcgca gaaggtgaag ttctgcaact
    41881 gatgaacgtt aacgatccgg acatcactga agaaaactac atgcgcgtta tctatagcaa
    41941 aaccgcgcgt ctgtttgagg ctgccgcgca gtgttccggg attctggctg gctgtacgcc
    42001 ggaggaggag aaaggcctgc aggattatgg gcgctatctc ggcactgctt tccagttgat
    42061 cgacgattta ctcgattaca atgccgatgg cgaacagtta ggtaaaaacg tcggcgacga
    42121 tctgaacgaa ggtaaaccga cgctgccgct gctgcatgcg atgcatcatg gcacaccaga
    42181 acaggcacag atgatccgta ccgccatcga acaaggtaac ggtcgccatc ttctggaacc
    42241 ggttctggaa gcaatgaacg cttgtggatc tcttgaatgg acgcgtcagc gcgccgagga
    42301 agaagcagac aaagccatcg cagcgttaca ggtgctcccg gacacccctt ggcgagaagc
    42361 actcatcggc ctcgcgcaca tcgctgttca acgcgatcgt taatcccctc ccttcatccc
    42421 gcgcagtgcg cgggatgatt ccagtaaatt ccataaaaac actaattcag cgctataaca
    42481 cctgaaaaaa agggcgatga atctttatat attctgaata ttcacactct ttacaggaac
    42541 tttttagcgc aataggccat caggagtata gtgatgcccg acagaagaag tgttctgaat
    42601 gaaagcgact aacttaagga gtgaggaaag atggaaagta atttcattga ctggcatccc
    42661 gctgacatca ttgcaggttt gcgcaagaaa ggaacgtcaa tggcggcgga atctcgcaga
    42721 aatggtttga gttcctcaac gctggcaaac gcattatcgc gcccatggcc gaaaggagag
    42781 atgattattg cgaaagccct gggaactgac ccctgggtta tctggccatc acgctaccat
    42841 gatccgcaga cccatgagtt tatcgacaga acgcagttga tgcgtagcta cactaaaccg
    42901 aaaaaatgag tggtctggcg gtagccccgc gaacggggct gccagctctc agacgattac
    42961 tcgcctttca cacgctcaat atttgcacct aaagcgcgca gtttgtcttc aatgcgttcg
    43021 tagccacgat cgatgtgata aatacgatca accaccgtcg tcccttccgc aatacagcca
    43081 gccagcacca ggcttgctga tgcacgcaga tcggttgcca taacctgtgc accagaaagt
    43141 ttttcaacac catgacaaat aacggtattg ctttcgattt cggcgtgcgc gcccatacgg
    43201 ctcagctctg gcacatgcat aaagcggttt tcaaagaccg tttcggtgat gaacccggtc
    43261 ccttcagcca ccaggttcaa cagcgtgaac tgggcctgca tatcggtcgg gaatgccgga
    43321 tgcggcgcgg tacgtacgtt aacagccttc ggacgtttgc catgcatatc caggctaatc
    43381 cagtcttcgc cgacttcgat gtccgctcca gcatcacgca gtttcgccag cacggcgtcc
    43441 agagtatctg gctgcgcgtt acggcagata attttgccac gagagatcgc cgccgccacc
    43501 aggaaagtac cggtttcgat acgatccggc agcacgcgat agacaccgcc gcctaaacgt
    43561 tccacacctt cgatgacgat acgatcggtg ccctgaccgc taattttcgc acccagcgta
    43621 atcaggaagt tcgcggtatc gacgatttcc ggttcacgcg ctgcgttttc aataatcgtg
    43681 gtgccttccg ccagggttgc agcacacatg atggtcaccg ttgcgccaac gctgacttta
    43741 tccatcacga tatgtgcgcc tttcagacga ccatcgacgg aagctttaac gtaaccttct
    43801 tccagtttga tggtcgcgcc taattgttcg aggccagaaa tgtgtagatc aaccggacga
    43861 gcaccgatcg tacaaccgcc aggtagtgaa acttgcccct gaccaaagcg cgctaccagc
    43921 ggccccagcg cccagataga agcacgcatg gttttaacca gatcgtaagg tgcgcagaat
    43981 acattaacgt cgcgggcatc aatatgcaca gaaccattac gttctacttt cgcacccagc
    44041 tggcttagca gtttcattga tgtatcgacg tctttcagtt tcgggacgtt ctggatctct
    44101 accggttctt ccgccagtag tgcggcaaaa agaataggca gagcagcatt tttagcgccg
    44161 gaaattgtga cttcgccctg gagcttcgtt ggcccctgaa cacgaaattt atccatttag
    44221 tttgttctca gttaacaatt catatccgct accggcgaat cgcccatagc tcaaaagccg
    44281 ttcagtttgc gatcgcgcgc ccactccgca ggggtatacg ctttgatcga cacagcatga
    44341 atgcggttat ccgcaatata ttccatcagc gggccataga ccgtctgctg ttttttaacc
    44401 cgactcatac cgtcaaacaa ctcacccacg gcaataacct gaaagtgact gccatcgccg
    44461 gaaacgtgga cttcctggag ggagagagcg ttcatcaaca cgctctgaat ttcattattt
    44521 tccatgggat cttcaatcat cagttaataa accagcgaaa catcttagag caaagttgcg
    44581 ctggcataaa taagcaaaaa gcctcgctga taaatcagac aaggctcgac ttgcaggcag
    44641 gtttgccgga caggcggtta acgccatatc cggcctgaaa aaatttaacg aggcagaaca
    44701 tcagcaggca aattatacaa tttcgcgagg gtatacactt tgtcgtttac cccctgaagc
    44761 gtcacgttgt tgccctgctt tttcgccaga tcgataagat ggagcagcag tgccagtccc
    44821 cccgtatcca cgcgggacac acggctaaga tcgatgcagg taatcccctt caccgcttcc
    44881 tcacgcattt cccaaagcgg tagcagaacg tcctgatcca gctctccgga taacgccagc
    44941 gtgtcacccg tctgcatcca gctcagtgac tcgctcatta ttttttctct tccagagtga
    45001 ttttctgttg agaaatcgat ttcagttgcg cagtcaggcc gtcgatacct ttggtacgca
    45061 gcagcgttcc ccactcgttt tgtttggtgg tgatcatact gacgccttca gcaatcatgt
    45121 cgtaagcttg ccagtttccc gtctgggagt ttttacgcca ctggaagtcc agacgcaccg
    45181 gcggacggcc attcgggtca ataatggtaa cgcgaatagg cacaatggtt ttatcgccca
    45241 gcggctgttc tggcgcaatc tgataggttt gaccgtgata catcgccagc gcctgaccgt
    45301 aagcctgctt caggtactca cggaaagcgg caaagtaggc ttcacgttga gcaggggtcg
    45361 cactcttgta atactggccc agcaccagcg caccggcgta tttcacctgt acgtatggca
    45421 gcagttcctg atcaacaatg gtacgcagat aatccgggtt ggcccgaatt tgcggttgct
    45481 cattcttcag gcgatcgaac gttttctgcg ccgcctcgtc catcagctta taaggattgg
    45541 tctggtctgc cgcggttgcc gcactcagag gtgcaatcac cagcaaagcg accatcatta
    45601 aacgtttaaa catgcgtcgg ttctcctgaa attatttcgt tgtacccaca ggttcagtgg
    45661 tttcattatt acctggcgca gcagctggcg catcgccact attcttattg tcatcgcctt
    45721 tactaccgta aaggaactga ccaatgagat cttccagcac catcgcagac ttggtgtcct
    45781 gaattgtatc gccatccttc aggatagcag tccccagttc cgggtcttca aaaccgacgt
    45841 ttaatgccag atattgttct cccagcaggc cggaagtacg aatgctcagc gaactggtat
    45901 caggaatgtg gttataacgt tgttcaattt ccagcgttac gcgcggcaga taggttttcg
    45961 ggtccagcgt aatatccgcc acccggccca caacaacacc accaatgctg accggagagc
    46021 gcgctttcag gccgccaatg ttatcgaacg tcgcataaag cgtgtaggtc gattctgtac
    46081 gtatggacgt cacgttcgcc gccttcaggc aaacaaacag cgccgccagc agtgctgcta
    46141 ataaaaagat acccacccaa atttcatttt ttttcgtttg catgaactca attcccaaac
    46201 atcaatgcgg tcagcacaaa atccagcccc agaacagcca gagacgagtg gacaacggtg
    46261 cgagtggttg cccggctaat cccggcagac gtcgggatgg cgtcgtagcc gttaaacaac
    46321 gaaatccacg tcaccgtgat ggcgaacacc acgctcttaa tcagacagtt gaccagatcc
    46381 atacgccagt cgacggcatt ttgcattgcc gaccagaaga acccgctatc aatgcctttc
    46441 caactgacgc cgaccagcga tccgccccag atccccacgg cgacgaagat aaccgtcaac
    46501 agtggtaatg aaataacccc agcccagaaa cggggagaaa taacccgacg cagcggatcc
    46561 accgccatca tctccatact ggagagttgc tctgtagcgc gcatcaggcc gatttctgcg
    46621 gttaacgccg aaccagcacg cccggcaaac aacagcgcgg caaccaccgg ccccagttca
    46681 cgcagtagcg ataacgccac cagcataccc agactggttt ccgcgctata agtggtcaga
    46741 accagataac cttgcagccc caacaccatt ccgatgaaca cgccagaaac tacaataatc
    46801 agcatcgaca ggacgccgac attatagagc tggcgcacca gcagcggcgc atgtttgcga
    46861 aattccggtt tgccgaccag cgcattgaat aacattaacc cggcccgccc gaacgttctc
    46921 agggttttaa tccctttatg tccgagcgac gccagcgcat ttaacagcat gagtggctta
    46981 actccctggt aaaagatcag cgtgataatc gccggcagga tagcggaacg gaacaggccc
    47041 gtcagctatc ccgtccagaa actgacgtac gcgcggatca ggattcgtct gcaacgcctg
    47101 ggcactgcca tgagcgacaa tttttttgtc cgccaggatc caggcgtgat ccgcaatact
    47161 taacacttcc ggcacatcgt gagaaaccac cacacaagtc acgcccagcg cgctgttcag
    47221 ctcagaaatc agcttcacca gtacgcccat ggtgatggga tcttgcccaa caaaaggttc
    47281 atcaaacatg atgagatccg gctccagcgc aatcgcacgc gccagcgctg cacgccgcgc
    47341 catcccaccg gaaagttcag aaggcattag tttagccgct ccacgcagcc ccacggcctc
    47401 cagcttcatc atcaccgtac tatgcaacaa tggcgcggga agttgggtat gttcgcgcag
    47461 tggataggcg acgttgtcaa atacgttcat atcagtgaac aacgccccgg actgaaataa
    47521 catgctcatc cgtttacgca ctgtatacag gcgcgaacga gacatcgccg gaatattctc
    47581 accatcgaaa aggatctcac catgatctgg tgcgatttgc ccgccaatca gacggagtag
    47641 cgtcgtttta ccgatgcccg atggccccat gatcgccgtg atcttccctc gcggcacggt
    47701 cagggaaata ttatcgaaga tgcagcgatt gccacgcgta aaactgacat cgcgcatatc
    47761 gactaaattc gccacagact gctccataat tcacccttcg tcttgcgttg attttctaag
    47821 catggcgctc aatttaacct tgaacccaac atatttacag aatattaccc gccgtggtta
    47881 gcgaaagctg gcatttgttt tactttttag ccgcataaag tcaaaattaa gaatccgtta
    47941 cggctttctg aaaatcttca gcggaccggc gagtatacct gaagaaagga cgttagatgc
    48001 ttttagctac ggcactgtta attgttggtt tacttttggt cgtttacagt gccgaccgcc
    48061 tggtttttgc cgcgtctatt ctttgccgaa cctttggcat cccgccgctg atcatcggca
    48121 tgacggtggt cagtattggt acatcgttac cagaaatcat cgtctcgctt gccgcgtctc
    48181 tgcacgaaca acgcgattta gccgttggta cagccctcgg ctcaaacatt atcaatatat
    48241 tgctgatcct cggtctggct gcgctggttc gtccttttac cgtccattcc gatgttctgc
    48301 gccgtgaatt acccttaatg ttgttggtca gcgtggtggc cggttccgta ctctatgacg
    48361 gacaacttag tcgaagcgat ggtatctttc tcctctttct ggctgtgcta tggctgctgt
    48421 tcattgttaa acttgcacgt caggggactg acagcctgac cagagagcag cttgcagagc
    48481 tgccgcgtga cggcggattg cccgtcgcgt ttttatggct cggcattgcg cttatcatca
    48541 tgccagtggc cacgcggatg gtggttgata acgccacggt gctggcgaat tactttgcca
    48601 tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg aaccagtctg ccggaactgg
    48661 caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat tgctgtcgga aatatcattg
    48721 gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata acgccaggag
    48781 agattgatcc actggcgtac agtcgtgact acagcgtgat gttgctggtg agcattattt
    48841 ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg gtattattaa
    48901 ctggcggatt tatcatatgg ctggcgatgt tgtactggtt atcgccaata ctcgttgaat
    48961 aactggaaac gcattatgtc gcacgtagag ttacaaccgg gttttgactt tcagcaagca
    49021 ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca atacatcaat
    49081 cagaatttca cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt tgtcgtcatg
    49141 gggatgggga aatcggggca catcgggcgc aaaatggccg caacgtttgc cagcaccggt
    49201 acaccttcat ttttcgtcca tcctagtgaa gccgcgcatg gtgatttagg catggtcacc
    49261 ccacaggatg tggtgattgc tatctctaac tcaggtgaat ccagcgaaat cacggcctta
    49321 attccagtgc ttaagcgtct tcacgtaccg ttaatctgca tcaccggtcg cccggagagc
    49381 agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga agcctgtccg
    49441 ttagggctgg caccgaccag cagcaccacc gccacgctgg ttatgggcga tgccctcgct
    49501 gtcgcgctgt taaaagcacg cggctttact gctgaagatt ttgcgctctc acacccaggc
    49561 ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac gggcgatgag
    49621 atcccgcatg ttaagaaaac ggccagtctg cgtgacgcat tgctggaagt tacccgcaaa
    49681 aatcttggta tgactgtcat ttgcgatgac aatatgatga ttgaaggcat ctttaccgac
    49741 ggtgatttac gccgtgtctt cgatatgggc gtggatgttc gtcagttaag tattgccgat
    49801 gtgatgacgc cggggggaat acgtgtgcgc cctggcattc tggccgttga ggcactgaac
    49861 ttaatgcagt cccgccatat cacctccgtg atggttgccg atggcgacca tttactcggt
    49921 gtgttacata tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa ggataaacaa
    49981 caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc gccgacgtta
    50041 tggcaaaagc agagaacatt cgtctgctga tcctcgacgt tgatggcgta ctgtcagatg
    50101 gcctgattta tatgggcaat aacggcgaag agctgaaagc gttcaatgtt cgtgacggtt
    50161 atggcattcg ttgtgcgctc acctctgata ttgaagtcgc tatcattacc gggcgaaagg
    50221 ctaaactggt agaagatcgt tgtgccacat tggggatcac tcacttgtat caggggcagt
    50281 caaacaaact gatcgccttt agcgatttgc tggaaaaatt ggcgattgcc ccggaaaatg
    50341 tggcttatgt tggcgatgat ctcatcgact gcccggtaat ggaaaaagtg ggtttaagcg
    50401 tcgccgtggc cgatgcccat ccactgttga tcccgcgcgc cgattacgtg acgcgcattg
    50461 ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt actcctggcg cagggcaaac
    50521 tggatgaagc caaagggcaa tcgatatgag taaagccaga cgttgggtta tcattgtgct
    50581 atcactggcg gttctggtga tgatcggcat taatatggcc gaaaaagacg ataccgccca
    50641 ggtggtcgtc aacaacaatg atcccaccta taaaagcgag catacggaca cgctcgtcta
    50701 taacccagaa ggggcactaa gctatcgatt gattgctcaa cacgttgaat attattccga
    50761 tcaggccgtt tcgtggttta cgcagccggt acttaccacg tttgataagg ataaaatccc
    50821 gacatggtcc gtaaaagcag ataaagccaa gctgaccaat gaccggatgc tctatttata
    50881 tggacacgtt gaagtcaacg cacttgtgcc agactctcaa cttcgcagaa tcacgacgga
    50941 taacgcgcag atcaatctgg tgacgcagga tgttacctct gaagacctcg tcacgttata
    51001 cggaacaaca tttaactcca gcggtctgaa aatgcgcggc aacttacgca gcaagaacgc
    51061 cgagctgatt gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa ctcagcctta
    51121 atcttgtgct tgccagctca cttctggccg ccagcattcc ggcatttgcc gtaaccggag
    51181 acactgatca gccgatccac attgaatcgg accagcaatc tcttgatatg caaggcaacg
    51241 tggttacctt taccggtaat gtcatcgtca cccagggcac catcaaaatt aatgccgaca
    51301 aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga agtgattgac ggatacggta
    51361 aaccggcaac gttctaccag atgcaggaca acggtaaacc cgttgaaggt cacgcttccc
    51421 agatgcacta cgaactggca aaagattttg tcgttctgac gggtaatgct tatctgcagc
    51481 aggtcgatag caacattaag ggcgataaga tcacttacct ggtgaaagag cagaaaatgc
    51541 aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct ggtgccgtcg cagctgcagg
    51601 acaaaaacaa caaaggccag accccggcac agaagaaggg taattaattc gttatggcaa
    51661 cattaactgc aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta gaagacgtca
    51721 gcctgaccgt caactccggg gaaattgtcg gtctgctggg gccaaacggt gccggtaaga
    51781 ccaccacttt ctacatggtt gtaggcattg tgccgcgcga tgcgggcaac atcattattg
    51841 atgatgacga tatcagtctg ctgcctctgc atgcacgcgc gcgccgcggt atcggctatc
    51901 tgccacagga agcctccatt ttccgtcgcc tcagcgttta cgataacctg atggcggtac
    51961 tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga ccgcgcgaac gagctgatgg
    52021 aagagtttca cattgagcac ctgcgtgaca gcatggggca gtcactctcc gggggtgaac
    52081 gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt ctgctcgacg
    52141 aaccgtttgc cggggttgac ccgatctcgg ttatcgatat taaacgcatc attgaacacc
    52201 tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa cgtgcgtgaa acactggcgg
    52261 tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat cgcccacggc acgcctacag
    52321 aaatcttaca agacgaacac gttaagcgtg tataccttgg ggaagacttc agactctgat
    52381 agggtagaag tttgcgacgt tttagcagga gagtacgatt ctgaacatga agcaaggttt
    52441 gcaactcagg cttagccaac aactggcgat gacgccacag ctccaacagg caattcgtct
    52501 gttgcagttg tcgacgctgg aacttcagca ggagctacag caggcgctgg agagtaatcc
    52561 gctgcttgag caaatcgaca ctcatgaaga aatcgacacc cgcgaaacgc aagacagtga
    52621 aacgctggac accgccgacg cgctcgaaca aaaagagatg ccggaagagc tgccgctcga
    52681 tgccagttgg gacaccattt acaccgctgg tacaccatcc ggcaccagcg gtgactacat
    52741 tgacgacgag ctgccggtct accagggcga aacgacgcag accttgcagg attacctgat
    52801 gtggcaggtc gagctgacac cgttttccga cactgaccgc gctattgcta cctctatcgt
    52861 cgatgccgtt gatgaaaccg gttatctgac tgtcccgctg gaagatattc tcgaaagtat
    52921 gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc cttaagcgga tccaacggtt
    52981 tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc ctgctaatcc aactctccca
    53041 attcgataag accacgccgt ggctggaaga ggccagattg atcattagcg atcatctcga
    53101 tctgttagcc aatcacgact tccgcacttt aatgcgcgtc acgcgtctga aagaagatgt
    53161 gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg cgccccgggc agtcgatcca
    53221 gactggcgaa cctgagtatg tcattccaga tgtgctggtg cgtaagcata acggtcactg
    53281 gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa atcaaccagc actacgcctc
    53341 gatgtgcaat aacgcgcgca acgatggtga cagccagttt atccgcagca atctgcagga
    53401 tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat acgctactgc gcgtgagtcg
    53461 ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt gaagaatata tgaaaccgat
    53521 ggtactggcc gatatcgccc aggctgtcga aatgcatgaa tcgacgatat ctcgcgtgac
    53581 cacgcaaaaa tacctgcata gtccacgagg catttttgaa ctgaagtatt tcttttccag
    53641 tcacgtcaat accgagggcg gcggcgaagc ttcctccacg gcgattcgtg cactggtgaa
    53701 gaaattaatc gcggcggaaa acccagcgaa accgttgagc gacagcaagt taacctcttt
    53761 gctgtcggaa caaggtatca tggtggcacg ccgcactgtt gcgaagtacc gagagtcttt
    53821 atccattccg ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat aaggaagaca
    53881 ctatgcagct caacattacc ggaaataacg tcgagatcac cgaggcactg cgcgaatttg
    53941 ttacagctaa atttgccaaa cttgagcaat attttgaccg aatcaaccag gtctatgttg
    54001 ttctgaaagt ggagaaagtc acccacacct cagatgcaac actgcatgta aacggtggtg
    54061 aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc ttaattgata
    54121 agctggcacg ccagttgacc aaacataaag ataaactgaa acaacactaa ttgtccgggc
    54181 aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta cggttaatgc
    54241 tccgagcctg ttccactgtt tgagtgggca ggttcttagg tgaaattatg acaaataatg
    54301 atacaactct acagcttagc agtgttctta acagggaatg tacgcgaagc cgcgtccact
    54361 gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa caacttagcc
    54421 taccgcctca ggtggttttt gaagctatcc tgacgcggga aaaaatgggc agtaccggta
    54481 tcggcaatgg tattgccatt ccgcatggca aacttgaaga agatactctg cgtgccgttg
    54541 gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc catcgacaac caaccggtgg
    54601 acttactttt tgccctgctg gtgccggcag accaaactaa aacgcacctg catactctgt
    54661 cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc gcagcccaga
    54721 gcgatgaaga gctgtatcaa atcattacgg ataccgaagg tactccggat gaagcgtagt
    54781 tattcggtaa tgtctctttt agacgttgtg aggagaaaca gtacatggta ctgatgatcg
    54841 tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa gatatgggtt
    54901 tttactgcgt ggataacctt cccgtagtgt tgttacccga tctggctcga actctggccg
    54961 atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa tatgccggag tcaccagaaa
    55021 tattcgaaca ggcgatgagt aacctgcctg acgctttctc accgcaacta ctgttcctgg
    55081 atgccgaccg taatacctta attcgtcgtt acagtgacac gcgccgactg catccgcttt
    55141 ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga aagcgatttg ctggagcctc
    55201 tgcgttcgcg agcggatctg attgtcgata cctcagaaat gtccgttcac gagctggctg
    55261 aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga actgaccatg gtctttgagt
    55321 ctttcggctt caaacacggt atccctatcg atgcagatta cgtctttgac gtgcgcttct
    55381 tgccgaaccc gcactgggat ccgaaactgc gtccaatgac aggtcttgat aaacctgtcg
    55441 ccgcgttcct cgaccgccac acagaagtac acaattttat ctaccagacg cgaagctatc
    55501 ttgagctatg gttacctatg ctggaaacca acaaccgtag ctacctgacg gtcgccattg
    55561 gttgtaccgg cgggaagcac cgttcggtgt atattgcaga gcaactggca gactacttcc
    55621 gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct ggaaaaacgt aaaccatgac
    55681 cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg catgcccggc ctgcaatgaa
    55741 gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg acgaaggcac
    55801 cgaggctgaa gccaacagcg ttattgcgct gctgatgttg gattctgcca aaggacggca
    55861 gattgaagtt gaagcgaccg gtccacagga agaggaagca ctggccgccg ttatcgccct
    55921 ctttaattct ggttttgatg aagattaatc ttcatcactt tgacatacaa ctcccttcaa
    55981 gctcccccga caataagaaa atcacgtact gaaatcgttc tcaatcaacg tcatttgtac
    56041 attttgtgcg cttttcactt tcagaagaac cttaagaaaa ccttaagagg cattgtttag
    56101 gttttgttta agttaatcga ccatactgga gatcgtcaga aaatatttcc aggagatggc
    56161 atgattcgct tatctgaaca aagtcccctg ggcaccggaa gacatcggaa atgttatgcg
    56221 catccggaag atgcccaacg ctgtatcaag attgtctacc atcgtggcga tggtggcgat
    56281 aaagagatcc gccgtgagtt aaagtactac gcgcatcttg gtcgccggtt aaaagactgg
    56341 agtggaatac cgcgctatca cggtaccgtt gagacggatt gcggaaccgg ttatgtctac
    56401 gatgtgattg ccgattttga cggcaagcct tccattacgc tgaccgaatt tgctgaacaa
    56461 tgccgttacg aagaagacat tgcacaactg cgccagctac tgaaacagct gaagcgttat
    56521 ttgcaggata accgtatcgt gacgatgtcg ctgaagccgc agaatatcct ctgtcaccgc
    56581 atcagtgagt cagaggttat cccggtcgtc tgcgacaaca ttggtgaaag cacattgatt
    56641 ccactggcga cctggtcgaa gtggtgttgt ctgcgtaagc aagagcggtt atggaaacga
    56701 tttatcgccc aaccggcgct ggctattgcc ctgcagaaag atctgcaacc tcgtgagagc
    56761 aaaacgctgg ccctcacttc gcgcgaagct taatccagct ggtgttgttg cataaacggc
    56821 tcaccgccta actgatacat ctgccgtaaa atccacgcct gacggctacg cacgtagccg
    56881 tcaggcgtgg agactttgaa acgaagtgga ttaggtaata cagctgccag taatgcagct
    56941 tccgaccggg taagtttgct cgcgggtttg tggaaataac gttgtgccgc agcttcgacg
    57001 ccaaacacac cgtcgccaaa ttcggcgata ttcaggtaaa ccgtcaggat acgctttttg
    57061 ctccagactg tttctatccc cagcgttaat ccagcttcca gcccttttcg tacccagcta
    57121 cgcccatccc ataaaaagag atttttggcc gtctgctgag aaatcgttga agcaccgcga
    57181 atacggtttt cattgcgctc gttgtgcgcc agggctttct caatggaagc gacatcaaag
    57241 ccccagtgct caggaaattt ctgatcttct gcggcaatca ccgccagtcc catccacggc
    57301 gagatttgat ccatactgac ccagtcagaa tgtgccacgt aacgaaaatt gccatgcagc
    57361 caggcgctga cctgtcgctc gaccattacc gctgagaagg gaacaggcgc aacgctaaac
    57421 aacgcgatgc ccccgcccca gaaaacggcg aggacaacca ttaaccgcaa aagaaaacgg
    57481 cgaacgaaac taaacaccgt taagcggctt ttactcattc agccagaacc agcacgcggg
    57541 aaaccagctt atcaatgccg ctcgccgctt ctgcaatgtt ctgcgccagc atatatgctg
    57601 gggtggtgac aatcttattg tcttcatcaa ccacgatatc atcgacagga cacggcacat
    57661 gctccgcgcc catctcttcc agtacttctg cggtatcgat atcagtaccg atggtcaaac
    57721 gcagcgggaa atcgaaaatt ttcggcagca tcgccggggc aatacacata aaaccaagcg
    57781 gttttccggc ctgatgcatc gcttgtgcca gcgcctttaa ttcacggtca acggtgcatt
    57841 cgctaccaag actggcaaaa ttgcttaaat tcttcgccgc gccaaccccc cccggcacaa
    57901 tcaacgcatc cagttcagcg gcatcggcct gggccagagg acggatttca ccgcgcgtta
    57961 ttcgtgccgc ttcaatcagc acattgcgcg tttccgtcat cgcttcgcca gttaaatggt
    58021 tgataacatc aacctgctgc ttatccggtg caaagcagac cgcctgcgca ccgctgcgtg
    58081 aaatagctaa caacgtcaac accgcttcat gaatttcaga accgtcatag acgccgcatc
    58141 cgctcagaat tacgccaatt ttcttcattg tgatcatcct ttcgcaattg actgaaacac
    58201 attaattttt ttaataaaaa tggtacgcat cacacattta actgattcat gtaacaaatc
    58261 atttaagttt tgctatctta actgcgtgcg gcctgaaaaa cagtgctgtg cccttgtaac
    58321 tcatcataat aatttacggc gcagccaaga tttccctggt gttggcgcag tattcgcgca
    58381 ccccggtcta gccggggtca ttttttagtg gcttttgcca cccacgcttt cagcacttct
    58441 acgtcgtgac gccactcttc tttcatctct tcaatccatt caccgacgtt atcttcccag
    58501 gcgggaaggt caggagactg aatttgctga cccagctgtt gcagatggcg taaccccact
    58561 gaccccgccg cacctttaat tttatgtcct tcctcaacaa tgcctttttt atcctgcgcc
    58621 gtcagattcg actccagcac gctgacatag cccggcatca tcttctcaaa caccgctaac
    58681 ccgtcggtga tcagcttcgg tcctacaagt tcgagatact gttccagcat gggaatatcg
    58741 agcaatgctt ctgatttact gttctcttct gtcgtcaccg tactcttctc atcatcctgg
    58801 gtatcccaga atttcttgat catcgcggtt agcgccggaa ccgaaagcgg cttactcagc
    58861 acatcatcca ttccagcatt gaggtactct tgtttgtctt tcagcacgtt agcggttaag
    58921 gccaccagcg gcggtaaatc ctcgcgcgga taacgcttcg tcagttcacg agagatatcc
    58981 agcccggtca tatctggcaa ctgaatatcc agcaacacca ggtcgtattc gcccggttta
    59041 aacatctcca gcgccgcctt gccggtcatg gcgacatcaa cgctgttacc taatttttcc
    59101 agcacagaac gcgcgacaat cacgttcagt tcaatgtctt ccaccagcag cacattcagc
    59161 gccggtaaag gcatatcgtc ttcatcaaac gcatcatcga cctcttctgc caccgacggt
    59221 gcgtggatcg tcaacgtaaa ggttgaacct ttgccctgtt cgctggtaac cgtaatatcg
    59281 ccgcccatat ttttcgccag acgacgagaa acggccagac caataccggt gccggtggca
    59341 ggtttaccgc catgactgtc tttcacctgg taatacatgg cgaagatttt atccagctca
    59401 tcctgcggaa tgccgatacc agaatcttcc acttcaaaat gcagcatatc gccttcatcg
    59461 tagcgcacgc gcacggtaac ctggccttgc tgggtgaatt tgacggcgtt actgatgagg
    59521 ttccacagga tctgccgtaa acgcgtcccg tcggtaatga cctgatgcgg taatggcagc
    59581 gtaggctcca ggttaaagcg caatcctttt tgttgcgcct gcaatgcgga gagattttcc
    59641 agatcggcaa ggaagctggt gaaatcaacc ggctgattat caagctggac cttgcgccgt
    59701 tccatcttat ccatgtcgat aatatcgtta aagatattcc ccagcgtgac ggccgaaaca
    59761 tggatagttt tgagatattt ttcctgctcg gcggtgagtt cggtatccag cagaatgcgg
    59821 ctcaggccga cgataccgtt cagcggtgta cgcaattcgt gactgatggt ggaaataaac
    59881 gtcgttttgt cgcggctggc gcgttcaagc gcatcctgat accgcttacg ctcggtaatg
    59941 tcgcgaccaa agcccatcaa accgtgacgt ttacccacgc ggtcgtagta cggcacttta
    60001 cggatttcaa agcaggcttt gcgcccgtcc gggtaatcca gccactgttc ataggtcagt
    60061 gacacattat gacggaacac tttttcatcg gtttcaatga cttttgcggc ggcttccggt
    60121 gagtaaacat cagcaggttt cagatgaacc agttgttttt cgctttttcc ggtcagcagc
    60181 tccatcgcgc ggttacagcc ggaaaactct ttatcttcgt tacgataaaa aaccaggtcg
    60241 ggtgaagcat caaggaagga acgtaagaat gaggattgct gctcgagctg aatttgtgtc
    60301 tcttcgcgct ctttgatttc aattttcagt tggccgaagg tttcctgcag ttctgcttcc
    60361 gctttttcac gaacggcaat ttcctgattt agctgggcaa tattatcttt taactgaacg
    60421 ttgaggctca aatcgcgctc gcgcatctcc tccagttttt gcaccagccg tgacagacgt
    60481 tgtcgtgact cctccagttg ctcgacgacc accgatagaa agtagaccgc ccacggcgta
    60541 atcagcaaac caaagaagat agaacgaata acatcaatgc tttcgacctg accatgcagc
    60601 accatggtta ccgccatttg taccacaatg gcaagaacga cgagggccag cgccagcaac
    60661 attgagaagc gcaccagacc taacttcatc atcaggtcaa cataatactg cgccagcaga
    60721 cgaatttgct tcattaggga attccttcac gacaacctgc cccaataata cccaattctg
    60781 acagttacgt tataaattgt gtgagaaatg cggaattccc ctcaacgacc tactccgttg
    60841 gaggtagcca cggacgtccc agcgccgatc cctgtacgcc atgttcattc agatagcgat
    60901 ccagctcgac cattcccgtc catcgatttt cgcaccacag cggagcaagc agcgtcgggc
    60961 gacgggcgct ggcggaaatg cggtggtaga tcacttccgg tggcgtatgg cgaatcattt
    61021 ctccggcagt gagcgtataa tcctccagtt caataccgtt caaacgcccc gcttcccagg
    61081 ctttcgccgt aatgctgcct ttcacaatat gcagcggatg cagctttatg ccgtccacgc
    61141 tggtttcaac cactcgctcc agcgtttgca aacattcagc ctgcccttcg ccaggcaggc
    61201 cgacaattaa gtgggaacac accttcagcc cgcgctgacg tgccagctgg gttgtacgct
    61261 gataacaggc aaaatcatgg ccgcggttga tgcgatgcag tgttttgtcg tgggcggttt
    61321 gtagccccag ctccagccac acttcgtaac cctggtcctt atattcgcaa agcagatcca
    61381 gcaccgcatc cggcacgcag tccgggcggg taccaacaca caaaccgaca atattggcct
    61441 ggctcaccgc ctgctgatac atagaacgca gcacctgaac ttccgcaaag gtgctggtat
    61501 acgcctgaaa gtaggccaga tagcgtttag cgcggttaac taaattcgcc tggtgcgcca
    61561 gttgctcggc aatggaacga tgctgctgcg cttcatcggc aaacgaggca acattacaga
    61621 atgtgcagcc accacgcccg atggtaccgt cacggttagg gcagctaaaa ccgccgtgca
    61681 gcgtcagctt atgcaccttt tgcccataac gacgggtgag atcaccacca aacatattga
    61741 ctaatttctg taactgcata atctgataga ccgcgccttg aaaagaggcc aaagcctgcc
    61801 atttttagct caattcggcg atgacctaga tcaatcgtcc cgccctgctt ttatcaactg
    61861 cataatcaat caaaattacc gaaatttcat gcataatcac ataaatcact tttgcttatc
    61921 ttgtgtcaga tttttttatc tccttatgga ttttaggcaa aaacagtagc atgaaacgtc
    61981 attaccaatt aaggcagtat aaaatgctgg ttttctcgtc agttcaaggc aggataaggg
    62041 ttaacacgcc tttatgacag tcaggaattg actgtttctc taacgactct ccttttagcc
    62101 ttaaagataa aatctatttt aatttcagcc atttaataaa gaattttgcg ctaaagcaca
    62161 tttctgtacc aataagcttg ccatttgacc tgtatcagct ttcccgataa gttggaaatc
    62221 cgctggaagc tttctggatg agcagcctgc tcatcatatt tatgcagtaa ttgagatccc
    62281 ctcttcaccg tattaaccga tgcgaaaagg acaacaaggg ggcgaatgcg aggcgcgcgt
    62341 atgacacgca aaccccgtcg ccacgctctt tctgtgcccg tgcgcagcgg ttcggaagtg
    62401 gggttcccgc agagcctggg ggaggttcac gatatgttgt acgataaatc ccttgagagg
    62461 gataactgtg gtttcggcct gatcgcccac atagaaggcg aacctagcca caaggtagtg
    62521 cgtactgcaa tacacgcact ggcccgcatg cagcaccgtg gcgcgattct cgccgatggt
    62581 aaaaccggcg acggttgcgg cttgctgtta caaaaaccgg atcgcttttt tcgcatcgtt
    62641 gcgcaggagc gcggctggcg tttagcaaaa aactacgctg tcgggatgct cttcctgaat
    62701 aaagatcctg aactcgccgc tgccgcacgc cgcatcgttg aagaagaact gcaacgcgaa
    62761 accttgtcga ttgtgggctg gcgtgatgtc cccactaacg aaggcgtgct gggtgaaatc
    62821 gccctctcct ctctgccacg cattgagcaa atttttgtga acgccccggc aggctggcgt
    62881 ccacgcgata tggagcgccg tctgtttatc gcccgccgcc gcattgaaaa gcgtctcgaa
    62941 gccgacaaag acttctacgt ctgtagcctg tcgaatctgg tgaacatcta taaaggtctg
    63001 tgtatgccgg cggatctgcc gcgcttttat ctggatcttg cggacctgcg tctggaatcg
    63061 gccatttgcc tgttccacca gcgcttctcc actaacaccg taccgcgctg gccgctggcg
    63121 caaccgttcc gctatctggc gcataacggt gaaatcaaca ccatcaccgg taaccgccaa
    63181 tgggcgcgtg cgcgtaccta taaattccag acgccgctga tccctgacct gcacgacgcc
    63241 gcgccattcg tcaacgaaac cggttctgac tccagttcca tggataacat gctggaactg
    63301 ctgctggcag gcgggatgga tatcatccgc gccatgcgcc tgttagtgcc acccgcctgg
    63361 cagaacaacc cggatatgga cccggaactg cgtgccttct ttgactttaa ctccatgcat
    63421 atggagccgt gggatggtcc ggcgggcatc gtgatgtccg atggtcgctt cgctgcctgt
    63481 aacctcgacc gtaacggtct gcgtccggca cgctacgtca ttaccaaaga caagctcatc
    63541 acctgcgcct ctgaagtcgg tatctgggat taccagcctg acgaagtggt cgaaaaaggc
    63601 cgcgtcgggc caggcgaact gatggtgatc gacactcgca gtgggcgtat tctgcactcg
    63661 gcagaaaccg atgacgatct gaaaagccgc cacccttata aagagtggat ggagaaaaac
    63721 gtccgccgac tggtaccgtt tgaagatctg cccgatgaag aagtgggtag ccgcgaactg
    63781 gacgacgaca cgcttgccag ctaccagaaa cagtttaact acagcgcgga agagctggac
    63841 tccgtaattc gcgtactggg cgaaaacggt caggaagcgg tcggttcgat gggcgatgat
    63901 accccattcg ccgtgctctc cagtcagccg cgcattattt acgactactt ccgccagcag
    63961 tttgcccagg tgactaaccc gccaatcgac ccgctgcgtg aagcgcatgt tatgtcgctc
    64021 gccaccagta tcggtcgtga aatgaacgtc ttttgcgaag cagagggcca ggcgcaccgt
    64081 ttaagcttta aatcgccgat tctgctctac tccgatttca aacagctcac gacgatgaaa
    64141 gaggagcact accgcgcaga tacgctggat atcacctttg acgtcactaa aaccacgctc
    64201 gaagcgacag tcaaagagct atgcgacaaa gccgaaaaaa tggtacgtag cggcaccgtg
    64261 ttgctggtgc tctccgaccg gaatatcgct aaagatcgcc tgccggttcc ggctccgatg
    64321 gcggttggcg cgatccagac ccgtctggtc gatcaaagcc tgcgttgcga tgccaacatc
    64381 atcgtcgaaa ccgccagcgc ccgcgatccg caccacttcg ccgtgttgct gggtttcggc
    64441 gcgacggcta tttatccgta ccttgcctat gaaacgctgg gccgcctggt agacacccac
    64501 gcgattgcca aagattatcg taccgtgatg ctcaactacc gtaacggcat caacaaaggc
    64561 ttgtacaaaa tcatgtccaa aatgggcatc tccaccatcg cctcttaccg ctgctcgaaa
    64621 ctgtttgaag cggtcggtct acacgatgat gtagtgggcc tgtgcttcca gggcgcggtc
    64681 agccgcattg gtggggcaag ctttgaagac ttccagcagg atctgctgaa tctgtcgaaa
    64741 cgtgcctggc tggcgcgcaa gccgattagc cagggcggcc tgctgaaata cgtccacggc
    64801 ggcgaatacc acgcctacaa cccggacgtg gtgcgcacgc tgcaacaggc ggtacaaagc
    64861 ggcgagtaca gcgactatca ggaatacgcg aagctggtta atgagcgtcc gacaaccacg
    64921 ctgcgcgatc tgctggcaat tacgccgggt gaaaacgcgg tcaacattgc tgatgttgaa
    64981 ccggcaagcg aactgtttaa acgctttgat accgccgcga tgtctatcgg cgcgttaagc
    65041 ccggaagccc acgaggcgct ggcggaagcg atgaatagca tcggcggtaa ctcgaactcc
    65101 ggtgaaggcg gcgaagaccc ggcgcgctat ggcactaaca aagtgtcgcg catcaagcag
    65161 gtagcttctg gtcgcttcgg cgttactccg gcgtatctgg tcaatgccga cgtcattcag
    65221 attaaagtcg cccagggcgc gaagccgggc gaaggcggtc agttgccggg cgacaaagtc
    65281 actccttaca tcgccaaact gcgctattcg gtgcccggag tgacgctgat ctccccgccg
    65341 ccgcaccacg atatctactc tatcgaagac ttagcgcagc ttattttcga cctcaagcag
    65401 gttaacccga aagcgatgat ctccgttaag ctggtgtccg aaccaggcgt tggcaccatc
    65461 gcgactggcg tggcgaaagc ttatgcggac ttgatcacca tcgcgggcta tgacggcggc
    65521 accggcgcaa gcccgctgtc gtcagtgaaa tatgcaggct gcccgtggga actcggtctg
    65581 gtggaaaccc aacaggcgct ggttgccaac ggcttgcgtc ataaaattcg tttgcaggtt
    65641 gatggcggcc tgaaaacggg tgtcgatatc atcaaagcgg cgattctcgg cgcagaaagc
    65701 ttcggcttcg gcactggccc aatggtggcg ctcggctgta aatatctgcg tatttgccac
    65761 ctgaacaact gcgccacggg tgtagcaact caggatgaca aactgcgtaa gaatcactat
    65821 cacggcctgc cgttcaaggt gactaattac tttgagttta tcgcccgtga aacccgcgag
    65881 ctgatggcac agctgggagt aacgcgtctg gtggatctga ttggtcgcac cgacctgctg
    65941 aaagagctgg acggtttcac cgccaaacag cagaaactgg cgctgtcgaa gctgctggag
    66001 actgccgagc cacatgcagg taagtcactc tactgcactg aaaacaaccc gccgtttgat
    66061 aacggcctac tgaacgcgca gttgctgcaa caggcgaaac cgtttgtcga tgaacgccag
    66121 agcaaaacct tctggttcga tatccgcaac accgaccgtt ctgtcggcgc gtcgctttca
    66181 ggctatatcg cccagacgca cggcgatcag gggctggcag ccgatcctat caaagcgtac
    66241 ttcaacggca ccgcaggcca gagtttcggt gtgtggaacg cgggcggcgt ggaactatac
    66301 ctgaccgggg atgccaacga ctatgtcggt aaaggcatgg cgggcggctt aatcgccatt
    66361 catcctccgg ttggttccgc cttccgcagc catgaagcaa gcattatcgg caacacctgc
    66421 ctgtatggcg cgaccggtgg tcgtctgtat gccgcaggcc gcgcgggtga acgtttcggc
    66481 gtgcggaact ccggtgctat caccgtggta gaaggcattg gcgacaacgg ctgtgaatat
    66541 atgacgggcg gtattgtctg tattctgggt aaaaccggcg ttaacttcgg cgcgggcatg
    66601 accggcggct tcgcttacgt tctcgatgaa agcggtgatt tccgcaaacg cgttaacccg
    66661 gaactggtcg aagtcttaag cgtcgacgat ctggcgatcc atgaagagca tctgcgcggt
    66721 cttatcaccg agcatgttca gcataccggt tctcagcgcg gtgaagagat tctggcgaac
    66781 tggtcaacct tcgccacgaa attcgcgctg gttaaaccga agtccagtga tgtaaaagca
    66841 ctgctgggtc accgtagtcg tagcgcagca gagttgcgcg tgcaggcgca gtaaggggta
    66901 gcaacaatga gtcagaatgt ttatcaattt atcgacctgc agcgcgttga tccgccaaag
    66961 aaaccgctga agatccgtaa aattgagttt gttgaaattt acgagccgtt ttccgaaggc
    67021 caggccaaag cgcaggctga ccgctgtctg tcgtgcggca acccatactg cgagtggaaa
    67081 tgcccggtac acaactacat cccaaactgg ctgaagctcg ccaacgaggg gcgtattttt
    67141 gaagcggcag aactgtcgca tcagaccaac acactgccgg aagtatgcgg acgtgtctgc
    67201 ccgcaagacc gtctgtgcga aggttcctgc accctgaacg atgagttcgg tgcggtgacc
    67261 atcggcaaca ttgagcgtta tatcaatgat aaagcgttcg aaatgggctg gcgtccggat
    67321 atgtccggcg tgaagcaaac cggtaaaaaa gtggcgatta tcggtgcagg cccggcaggt
    67381 ctggcgtgtg cggatgtcct gacgcgtaac ggcgtaaaag ccgttgtctt cgaccgtcat
    67441 ccagaaattg gcgggctgct gaccttcggt attccggcct tcaagctgga aaaagaggta
    67501 atgacgcgtc gccgtgaaat cttcaccggc atgggtattg aattcaaact caataccgaa
    67561 gtgggccgcg acgtacagct ggacgatctg ctgagtgatt acgatgccgt gttccttggc
    67621 gttgggactt atcagtcaat gcgcggcggg ctggaaaacg aagacgccga tggcgtgtac
    67681 gcagcgctgc cgttccttat cgccaacacc aaacagttaa tgggctttgg tgaaacccgc
    67741 gacgaaccgt tcgtcagcat ggaaggcaaa cgcgtggtag tacttggcgg tggcgacact
    67801 gcgatggact gcgtgcgcac gtctgtacgc cagggggcaa agcacgttac ctgtgcctat
    67861 cgtcgtgatg aagagaacat gccgggttcc cgccgcgaag tgaaaaacgc gcgggaagaa
    67921 ggcgtagagt tcaaattcaa cgtccagcca ctggggattg aagtgaacgg taacggcaaa
    67981 gtcagcggcg taaaaatggt acgtaccgaa atgggcgaac cggatgccaa aggccgtcgc
    68041 cgcgcggaga tcgttgcagg ttccgaacat atcgttccgg cagatgcggt gatcatggcg
    68101 tttggtttcc gtccacacaa catggaatgg ctggcaaaac acagcgtcga gctggattca
    68161 caaggccgca tcatcgctcc ggaaggcaac gacaacgctt tccagaccag caacccgaaa
    68221 atctttgctg gcggcgatat cgtccgtggt tccgatctgg tcgttaccgc catcgccgaa
    68281 ggtcgcaaag cggcagacgg cattatgaac tggctggaag tttaagctct gtcccagcgc
    68341 cgcctcctca gaaaagagtc ggcgctgaaa ttctcctttc tttctggaaa tcgcctcgca
    68401 taatctgaag aaaaccaatg taatacgctc atctggattg ttaccggtag atgcccggcg
    68461 tatagctgat tccattattc aacgcagcgc catataaagg gacagataat ggaatcgctc
    68521 tcagagagaa cctcaacagg ctaccagcaa atccacgacg gcattattca tctggtcgat
    68581 agcgcccgaa cggaaacggt acgtagcgtt aacgcgctga tgaccgcgac gtactgggaa
    68641 attggccgac ggattgtcga atttgaacaa ggtggtgagg ccagagcagc ttatggtgca
    68701 cagttgattg atcggctatc gaaggattta agtcgacggt ataaacgtgg ggtctctgca
    68761 aaaaacttac gccaaatgag gcttttttac ctcttttttc aacatgttga aattcgccag
    68821 acagtgtctg gcgaattaac accattggga attccgc
//
LOCUS       DAJTYF010000006        68222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTYF010000006 DAJTYF010000000
VERSION     DAJTYF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.006"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            37..285
                     /locus_tag="OO590_000490"
                     /pseudo
     CDS             37..285
                     /locus_tag="OO590_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(332..1090)
                     /gene="deoR"
                     /locus_tag="OO590_000491"
     CDS             complement(332..1090)
                     /gene="deoR"
                     /locus_tag="OO590_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6635982.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWLIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1148..1744)
                     /gene="ybjG"
                     /locus_tag="OO590_000492"
     CDS             complement(1148..1744)
                     /gene="ybjG"
                     /locus_tag="OO590_000492"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6635983.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2029..3255
                     /gene="mdfA"
                     /locus_tag="OO590_000493"
     CDS             2029..3255
                     /gene="mdfA"
                     /locus_tag="OO590_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR6635984.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3296..3581)
                     /locus_tag="OO590_000494"
                     /pseudo
     CDS             complement(3296..3581)
                     /locus_tag="OO590_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3667..4482)
                     /gene="ybjI"
                     /locus_tag="OO590_000495"
     CDS             complement(3667..4482)
                     /gene="ybjI"
                     /locus_tag="OO590_000495"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6635985.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4482..5690)
                     /locus_tag="OO590_000496"
     CDS             complement(4482..5690)
                     /locus_tag="OO590_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6635986.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5774..6310
                     /gene="rcdA"
                     /locus_tag="OO590_000497"
     CDS             5774..6310
                     /gene="rcdA"
                     /locus_tag="OO590_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6635987.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6485..8170)
                     /locus_tag="OO590_000498"
     CDS             complement(6485..8170)
                     /locus_tag="OO590_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6635988.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            8440..8817
                     /locus_tag="OO590_000499"
     CDS             8440..8817
                     /locus_tag="OO590_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6635989.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8847..9104)
                     /gene="grxA"
                     /locus_tag="OO590_000500"
     CDS             complement(8847..9104)
                     /gene="grxA"
                     /locus_tag="OO590_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6635990.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9264..9551
                     /locus_tag="OO590_000501"
     CDS             9264..9551
                     /locus_tag="OO590_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6635991.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9535..10258
                     /gene="nfsA"
                     /locus_tag="OO590_000502"
                     /pseudo
     CDS             9535..10258
                     /gene="nfsA"
                     /locus_tag="OO590_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10319..11221
                     /gene="rimK"
                     /locus_tag="OO590_000503"
     CDS             10319..11221
                     /gene="rimK"
                     /locus_tag="OO590_000503"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6635992.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11309..11785
                     /locus_tag="OO590_000504"
     CDS             11309..11785
                     /locus_tag="OO590_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6635993.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12137..13249
                     /gene="potF"
                     /locus_tag="OO590_000505"
     CDS             12137..13249
                     /gene="potF"
                     /locus_tag="OO590_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6635994.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13344..14420
                     /gene="potG"
                     /locus_tag="OO590_000506"
     CDS             13344..14420
                     /gene="potG"
                     /locus_tag="OO590_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6635995.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            14487..15440
                     /gene="potH"
                     /locus_tag="OO590_000507"
     CDS             14487..15440
                     /gene="potH"
                     /locus_tag="OO590_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6635996.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15437..16282
                     /gene="potI"
                     /locus_tag="OO590_000508"
     CDS             15437..16282
                     /gene="potI"
                     /locus_tag="OO590_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6635997.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16342..16830
                     /locus_tag="OO590_000509"
     CDS             16342..16830
                     /locus_tag="OO590_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6635998.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16871..17998
                     /gene="rlmC"
                     /locus_tag="OO590_000510"
     CDS             16871..17998
                     /gene="rlmC"
                     /locus_tag="OO590_000510"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6635999.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18173..18904)
                     /gene="artJ"
                     /locus_tag="OO590_000511"
     CDS             complement(18173..18904)
                     /gene="artJ"
                     /locus_tag="OO590_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6636000.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19196..19864)
                     /gene="artM"
                     /locus_tag="OO590_000512"
     CDS             complement(19196..19864)
                     /gene="artM"
                     /locus_tag="OO590_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6636001.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19864..20580)
                     /gene="artQ"
                     /locus_tag="OO590_000513"
     CDS             complement(19864..20580)
                     /gene="artQ"
                     /locus_tag="OO590_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6636002.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20587..21318)
                     /gene="artJ"
                     /locus_tag="OO590_000514"
     CDS             complement(20587..21318)
                     /gene="artJ"
                     /locus_tag="OO590_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6636003.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21336..22064)
                     /gene="artP"
                     /locus_tag="OO590_000515"
     CDS             complement(21336..22064)
                     /gene="artP"
                     /locus_tag="OO590_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6636004.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22282..22797)
                     /locus_tag="OO590_000516"
     CDS             complement(22282..22797)
                     /locus_tag="OO590_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6636005.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23446..25215
                     /locus_tag="OO590_000517"
     CDS             23446..25215
                     /locus_tag="OO590_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6636006.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25426..25749
                     /locus_tag="OO590_000518"
     CDS             25426..25749
                     /locus_tag="OO590_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6636007.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25746..26576
                     /gene="amiD"
                     /locus_tag="OO590_000519"
     CDS             25746..26576
                     /gene="amiD"
                     /locus_tag="OO590_000519"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6636008.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26573..27586)
                     /locus_tag="OO590_000520"
     CDS             complement(26573..27586)
                     /locus_tag="OO590_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6636009.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVLTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27685..29115)
                     /locus_tag="OO590_000521"
     CDS             complement(27685..29115)
                     /locus_tag="OO590_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6636010.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANKHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29126..30127)
                     /gene="ltaE"
                     /locus_tag="OO590_000522"
     CDS             complement(29126..30127)
                     /gene="ltaE"
                     /locus_tag="OO590_000522"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6636011.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30164..31882)
                     /gene="poxB"
                     /locus_tag="OO590_000523"
     CDS             complement(30164..31882)
                     /gene="poxB"
                     /locus_tag="OO590_000523"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6636012.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(32015..32983)
                     /gene="hcr"
                     /locus_tag="OO590_000524"
     CDS             complement(32015..32983)
                     /gene="hcr"
                     /locus_tag="OO590_000524"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6636013.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32995..34647)
                     /gene="hcp"
                     /locus_tag="OO590_000525"
                     /gene_synonym="priS"
     CDS             complement(32995..34647)
                     /gene="hcp"
                     /locus_tag="OO590_000525"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6636014.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34791..35690)
                     /gene="lysO"
                     /locus_tag="OO590_000526"
     CDS             complement(34791..35690)
                     /gene="lysO"
                     /locus_tag="OO590_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6636015.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36147..36842)
                     /gene="aqpZ"
                     /locus_tag="OO590_000527"
     CDS             complement(36147..36842)
                     /gene="aqpZ"
                     /locus_tag="OO590_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR6636016.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     VSGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37267..38925
                     /locus_tag="OO590_000528"
     CDS             37267..38925
                     /locus_tag="OO590_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6636017.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38922..39878)
                     /locus_tag="OO590_000529"
     CDS             complement(38922..39878)
                     /locus_tag="OO590_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6636018.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            40029..41144
                     /gene="macA"
                     /locus_tag="OO590_000530"
     CDS             40029..41144
                     /gene="macA"
                     /locus_tag="OO590_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6636019.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41141..43087
                     /gene="macB"
                     /locus_tag="OO590_000531"
     CDS             41141..43087
                     /gene="macB"
                     /locus_tag="OO590_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6636020.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(43160..43384)
                     /gene="cspD"
                     /locus_tag="OO590_000532"
     CDS             complement(43160..43384)
                     /gene="cspD"
                     /locus_tag="OO590_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6636021.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43707..44027
                     /gene="clpS"
                     /locus_tag="OO590_000533"
     CDS             43707..44027
                     /gene="clpS"
                     /locus_tag="OO590_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6636022.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            44058..46334
                     /gene="clpA"
                     /locus_tag="OO590_000534"
     CDS             44058..46334
                     /gene="clpA"
                     /locus_tag="OO590_000534"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6636023.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46737..46824)
                     /locus_tag="OO590_000535"
     tRNA            complement(46737..46824)
                     /locus_tag="OO590_000535"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46788..46790),aa:Ser,seq:gga)
     gene            complement(47078..47296)
                     /gene="infA"
                     /locus_tag="OO590_000536"
     CDS             complement(47078..47296)
                     /gene="infA"
                     /locus_tag="OO590_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6636024.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47581..48285)
                     /gene="aat"
                     /locus_tag="OO590_000537"
     CDS             complement(47581..48285)
                     /gene="aat"
                     /locus_tag="OO590_000537"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6636025.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48327..50048)
                     /gene="cydC"
                     /locus_tag="OO590_000538"
     CDS             complement(48327..50048)
                     /gene="cydC"
                     /locus_tag="OO590_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6636026.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTQLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(50049..51815)
                     /gene="cydD"
                     /locus_tag="OO590_000539"
     CDS             complement(50049..51815)
                     /gene="cydD"
                     /locus_tag="OO590_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6636027.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51938..52903)
                     /gene="trxB"
                     /locus_tag="OO590_000540"
     CDS             complement(51938..52903)
                     /gene="trxB"
                     /locus_tag="OO590_000540"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6636028.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53448..53942
                     /gene="lrp"
                     /locus_tag="OO590_000541"
     CDS             53448..53942
                     /gene="lrp"
                     /locus_tag="OO590_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6636029.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            54077..58183
                     /gene="ftsK"
                     /locus_tag="OO590_000542"
     CDS             54077..58183
                     /gene="ftsK"
                     /locus_tag="OO590_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6636030.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            58338..58949
                     /gene="lolA"
                     /locus_tag="OO590_000543"
     CDS             58338..58949
                     /gene="lolA"
                     /locus_tag="OO590_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6636031.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58960..60303
                     /gene="rarA"
                     /locus_tag="OO590_000544"
                     /gene_synonym="mgsA"
     CDS             58960..60303
                     /gene="rarA"
                     /locus_tag="OO590_000544"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6636032.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60394..61686
                     /gene="serS"
                     /locus_tag="OO590_000545"
     CDS             60394..61686
                     /gene="serS"
                     /locus_tag="OO590_000545"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6636033.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61926..64370
                     /gene="dmsA"
                     /locus_tag="OO590_000546"
                     /pseudo
     CDS             61926..64370
                     /gene="dmsA"
                     /locus_tag="OO590_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64381..64998
                     /gene="dmsB"
                     /locus_tag="OO590_000547"
     CDS             64381..64998
                     /gene="dmsB"
                     /locus_tag="OO590_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6636034.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     HYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            65000..65863
                     /gene="dmsC"
                     /locus_tag="OO590_000548"
     CDS             65000..65863
                     /gene="dmsC"
                     /locus_tag="OO590_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR6636035.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(65899..66525)
                     /locus_tag="OO590_000549"
     CDS             complement(65899..66525)
                     /locus_tag="OO590_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6636036.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            66840..67988
                     /locus_tag="OO590_000550"
     CDS             66840..67988
                     /locus_tag="OO590_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6636037.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT        16954 a        16867 c        17862 g        16539 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcaccg tgaccatacc gcgtgggcag
       61 ctgaaaaacc tgaaggcgag ctacacgtta acggaaccgc agcttaccgc accgctgaaa
      121 aaaggtcagg ttgtcgggac cattgatttc cagcttaacg gtaaatccat tgagcagcgt
      181 ccgctgatcg tgatggaaaa tgtggaagag ggcggattct ttggtaggat gtgggatttc
      241 gtgatgatga aattccatca gtggttcggc agctggttct cttaatcttc tgataaccgg
      301 atggcgcgaa acgtcatccg gttatacgtc attaatacat caacttaata cggtgcgtct
      361 gcgcgtactt cacatactca tcttccgggc aacaatcact caccacaata tcaaagcgtt
      421 tcaggtcacc catgcgcgcc ggacgcacct tgccaaattt actgtggtcg acaaccagca
      481 catgcttttg cgccatcgac atggcccagt gttttaccgg caactcttca agattaaaac
      541 aggtagcgcc tttactgaca tgcacgcccg ccgcggaata aaaagcgata tccgggcaaa
      601 aattattcaa cgtttgctga aaatcgatgg gtttgaaaat ggcgttgctg gcgtgaaatt
      661 caccaccgca cagaaatgcg cggcaatggg gtttctcttt cagcgccaga aaggtattta
      721 gcgaataaca aacggcggta aaagggattt cattatcaat cgcttcaata agccacggcg
      781 tggtggtgcc acagtcaaaa aagagggtct gatcgggttc taccagtgtc gcagccagtt
      841 ttgccgcccg gcgtttttct tccaccaggc gggatttttg atcgcttaac aggtaatggc
      901 tggcactgcg cggttccaga acaatatagc cgccgagcaa aacgacgggc gcactgtggt
      961 tgttcagatc gcgacgaatc gtcatctccg aaaccccaag cagggcggcg gcgtctttaa
     1021 gatgtaactt atcgctgcgt tttaattctt gcagcagctg cccgatacgc tcttcgcgac
     1081 gtgtttccat aatccctctg aatagttatt gaagcgagcc gctcaatact acacttttta
     1141 gcagagatca gtcacgcacc cagcctttgc ggatcggtaa tgcaaaacag acgcgatacc
     1201 acgattgcag acgttgatag agtttatgcc ccatcgcttg ccagataatc tgggcactaa
     1261 ggcagccaat catacctgcc agcaatccac cgagcatatc cagcggccag tggacgccaa
     1321 gataaacgcg cgaccaggca atgacgacgg ccagcaccat taaaagtgag ccggaccaca
     1381 ggcgatgcca gcataaaaat gccagtgcaa aggtgaaaat caccgtaccg tgatcgctcg
     1441 ggaatgagtc atccgccgca tgatgcagga agttatagcc gatattttcg acaaagggtc
     1501 ggtcgtgcgg aaaaagatgt cccatcgtcc aggacacaaa caggctgacc gccagcgcga
     1561 tagcgatttt tatcaccagt tgccgttgtg ctgtaagccc ccacaaccaa agtaccgcgg
     1621 ccagcaacgg caccacggta atcaaatctt tagcaataaa aatcgccaac gagatcatcc
     1681 acggagccga gtctggcgtc gcgttaataa gagagaatag agagagattc aaattttcca
     1741 gcataacttc ccgacgcaaa gtgattaaaa gggagccaat acaggcaagt cgttgagaat
     1801 aaagtgcagg ttaaactggg taaagcggca tcgtcttatt tccctcaagc ggcctgttta
     1861 cggtgggtga ttgtaacggg cataggttaa ataaaactta aagaaagcgt agctatactc
     1921 gcaataatgt aagaatgtgc ttaaccgtgg tttcagctac aaaattcgct ttctcgttag
     1981 ctgcgctttt attaaactct gcgcgattat tattggcgaa gaaattgcat gcaaaataaa
     2041 ttagcttccg gtgccaggct tggacgtcag gcgttacttt tccctctctg tctggtgctt
     2101 tacgaatttt caacctatat cggcaacgat atgatccaac ccggtatgtt ggccgtggtg
     2161 gaacaatatc aggcgggcat tgattgggtt cctacttcga tgaccgcgta tctggcgggc
     2221 gggatgtttt tacaatggct cctggggccg ctgtcggatc gtattggtcg ccgtccggtg
     2281 atgctggcgg gtgtggtgtg gtttatcgtc acctgtctgg caatattact ggcgcaaaac
     2341 attgaacaat tcaccatgtt gcgcttcttg cagggcataa gcctctgttt cattggcgct
     2401 gtgggatacg ccgcaattca ggaatccttc gaagaggcgg tttgtatcaa gatcaccgcg
     2461 ctgatggtga acgtggcgct gattgctccg ctacttggtc cgctggtggg cgcggcgtgg
     2521 atccatgtgc tgccttggga ggggatgttt gttttgtttg ccgcattggc agcgatctcc
     2581 tttttcggtc tgcaacgagc tatgcctgaa accgccacgc gtataggcga gaaactgtca
     2641 ctgaaagaac tcggtcgtga ctataagctg gtgctgaaga acggccgctt tgtggcgggg
     2701 gcgctgggat tcgttagcct gccattgctg gcgtggatcg cccagtcgcc gattatcatc
     2761 attaccgggg agcagttgag cagctatgaa tatggcttgc tgcaagtgcc tattttcggg
     2821 gcgttaattg cgggtaactt gctgttagcg cgtctgacct cgcgccgcac cgtacgttcg
     2881 ctgattatta tgggcggctg gccgattatg attggtctgt tggtcgctgc tgcggcaacg
     2941 gttatctcat cgcacgcgta tttatggatg accgccgggt taagtattta tgctttcggt
     3001 attggtctgg cgaatgcggg actggtgcga ttaaccctgt ttgccagcga tatgagtaaa
     3061 ggtacggttt ctgcggcgat gggaatgctg caaatgctga tctttaccgt cggtattgaa
     3121 atcagcaaac atgcctggct gaacgggggc aacggactgt ttaatctctt caaccttgtc
     3181 aacggaattt tgtggctgtc gctgatggtt atctttttaa aagataaaca gatgggaaat
     3241 tctcacgaag ggtaaaaaaa tgcctgattg atttgtgcga tcaggcattc tcgaattaat
     3301 ggtgatggtc gtcaatctgg tgttcgataa ccatcccttc acctacgctg gcaagatggc
     3361 gaacataagg atgcgggcgg taagccggag ctggcacagg agccacatag acggtttgcg
     3421 gcacagtcgc gacgctgaca cgcttgtgga acgctgactg aatcaggaac cacaaccact
     3481 ttgtatccac tcggcacatc gacggtgaca ctttgcgcca tcgccacacc agtaaagccc
     3541 attaaaagcg cgcccaacag tagacaattt ttcataatca tttcgcctga aataatgcag
     3601 cgggttaatt tccgcgaatt atgcagcctt cattcaggca ttgtgttgcc gggcgtgctg
     3661 ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact ttatcgatca catccagtac
     3721 gccttcacgg ttattggagc ctgcccggta ttttgccgct gcgacgaccg cgctgccggc
     3781 atttttcatt gcaaaactaa agcctgcctg acgcagcatc tcaatatcgt taccgccatc
     3841 gccaaagacc accacttcgc tgtcgtctat tccccataat ttctgcagtt ggcgaaggcc
     3901 attggcttta tgtacgccgg ggataatcag atcgatgctg ccgttgccgg tgtggactgg
     3961 caccataata tcgccgatgg cctcatgtaa tgctttttgt acttgtggaa tcagttcatc
     4021 ggaaagattc aggccaaact taaagaaaat atcttctaag ttgtcaaagt tatcgacgta
     4081 ttccagacgg tgataataca tttccgccac cgttttcatg gcatcgtcat attttttgag
     4141 tgtataggcg ctattttttc cgcaggcaat aatttccact tccgggcgcg tcagcaaatg
     4201 ttccacgaca gtagcaaacg catccttcga cagctcgcca ttaaaaacat ctttgccttc
     4261 actcactacc cagccgccgt tttcagccac aaaggcaatt tcattcgcaa tttcagggaa
     4321 gaaagagatc aactgataat attgattccc gctggcgacc acaaagcgaa tgccttgtgc
     4381 tttcatttgc tgatactgag ccataaaccg ctcacggtta taggtttttt gatcgcttaa
     4441 gaaagtacca tccatgtcta ccgcaattaa tttaatactc atcaactatt ctccatcgcc
     4501 gtctgcgttt tggtatcggg tttggcgacg gctttcgcga caatagccgc gagaataacc
     4561 agcgccagta caaccagcat tgcactacgt aatccataat gttcgccgag atagcccagc
     4621 agcggcggcc cgacgaggaa agccagataa ccggtcgttg ctaccacact gacgcgggtt
     4681 ggtgcatcgg ggccggtatc gctggcggca gaaatggtca gcgggaagcc cagcgaggca
     4741 cccagtcccc aaagtacaac agacactcct gcgacccagg cgctatcgac aaaaataatc
     4801 agcccaatac ccaacgcccc cattagtgca ctggcccgaa ccacggcaac gcgactgtaa
     4861 cggtcgatga accaaccgcc ggtgaagcgt ccaacggtca tccccagggt aaaaccggca
     4921 taaatcagtg agccggaagt ggggctaaaa ccgtgaccat caaccattaa taagggtaac
     4981 cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca ctacaacacc aatcaacagc
     5041 aactggatat cgcgataaaa aggaatgcct ttttcgccat gctgggtgcc atcggcagca
     5101 tttttgcccg taccgtcagg gattgcctga atggcgatat aaataggcgc gatacctacc
     5161 agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa aggccgtcag tgccatcccg
     5221 acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt gcatcatcgg caaaaccgtt
     5281 ttattcattt ctcgctcaac ggcggcacct tcaacgttta tcgccacttc cgcagaacca
     5341 aaacttgccc caaagacgcc gagaccaacg gcaaagagca ggggcgatgt cagccagagt
     5401 gccagactta atatcatcat cccgatcaat gcgcaggaca tcgtgaccag gatgacatta
     5461 cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa taccgctcat cgaaccgatc
     5521 gacagaccaa agagaacacc gcccatttca gcgatcgaga cagagagaat atcgcggata
     5581 gcaggcgtac gggttgccca ggacgccatt aacaggcctg gcaaaaagaa gaacataaac
     5641 agcgccctgg ttcggcgttt caatgcatta cgtgaagaat ttacggtcat agatcacgtc
     5701 aaaataagaa gagggaagac aacattagca aggttgtgta catttgtaca caattgcaga
     5761 cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg cgagaaaaaa ttatccaggc
     5821 cacgctggag gcggtgaaac tttacggaat acatgctgtt acgcaccgca aaattgctac
     5881 ccttgccggg gtaccgttgg gatcgatgac ctactatttt tcaggaattg atgagttgtt
     5941 gctggaagcg ttcagcagtt ttactgagat tatgtcccgg caatatcagg cattttttag
     6001 cgatgttagt gatgctcaag gcgcatgcca ggctatcacc gatatgatct acagctcaca
     6061 ggttgcaacg ccggataata tggagctgat gtaccagctc tacgcgctgg ctagccgaaa
     6121 accgctatta aaaacggtaa tgcaaaactg gatgcagcgc agtcagcaaa cgctcgaaca
     6181 atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt attgagggga tgacgctgca
     6241 ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt ttgaggatgg ttgagagggt
     6301 tgcagggtag tagataaatt ttagataaca aaaaacccat caaccttgaa ccgaaatggc
     6361 ggggttgatg ggctccacaa aatggggaca tcaaagaaaa gcagtggcac taattaagac
     6421 tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat ttcattttca gggcaacttc
     6481 agttttatcc taatcctggc cataccatga cgatgattgt ccctgccagc gtcagcagga
     6541 cgttggtgat tgcgtaggtg cccgcatagc ccagcgcagg gatgttactg cgagctgtat
     6601 cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc catcattgcg ccgaacaaca
     6661 gtgcgcggtt cattcgcaat acataagcac cgaacaagaa acagataacc acgggcacaa
     6721 gactgacaat taatccggca atcaacatct gaccgccaat cgcgcccagg ccgttattaa
     6781 taccgctacc ggcgctcaga ccaacgcctg ccataaacac catcaagccg aactctttca
     6841 ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg gtggttagca cgcataaagc
     6901 ccagcataat tccggcgaat aacaacccgg cagcgttccc catgccgaaa ctgaatgtgc
     6961 tgaactggaa ggtgatcatc ccgatcatca gcccaataac aaagaaggcg caaaatgcca
     7021 gcaggtcagt gacctggctg tgaatcgaga taaagccgat gcgatcggcg atggttttta
     7081 cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt gttaagcacg acgttgtcat
     7141 ctatcggcat ctcaatctgg ctacgaatga cgcggttaag gaagcaaccg tgatcggtca
     7201 acttcagttg tgcgagacgt ttacctacag cgttatggtt tttaacgacc acttcttcag
     7261 tgacgatacg catgtcgaga aggtcacgat cgaaaacttc tttaccgtta cggaagctgg
     7321 gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc tatttcatcg cccatttgta
     7381 gcacggcatc accgtctgga tttgccagaa tcccgttacg tcgaatacgt tcaatgtagc
     7441 agccggtttg tcgataaata cccagttcac gcagattttt gccgtcggtc caggccacca
     7501 gctccgggcc gacgcgatag gcgcggatca ccggtaaata aaccttacgg ttggcatcag
     7561 tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt ctgtaagtcc tgatgctgca
     7621 atttcggcaa gtaacgcgca ccaacaatca aactcaccag accgattaaa taggttaagg
     7681 catacccgag gctcagatta tccagtgcca gtgagagctg cctgctttcc atgccggaat
     7741 gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga cgtcatagag cctgctaaca
     7801 taccggccgt caggccaata tcccagccaa acagcttacc taaccctaag gcgatcacca
     7861 gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt tttcccatcg cgaaaaaaaa
     7921 tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa aataaacagc ataaagccaa
     7981 gattaagcgc atcggtgtta atgctgaaat gttgttggcc taataacagc gatacgacta
     8041 aaacgccaat ggaattaccc agttggatcg aaccaagtcg taactttccg agacatagcc
     8101 caagcgcgag gaccacaaat aataacagaa tgtaattccc atttaacaat tcggcgacgt
     8161 ttatattcac ggaggctaac ttcttgttta ctagtaagct gttgaaagaa atggtaattt
     8221 acgataatgt gttttaccag aattcagggt gcagattcat tcagcgcacc taaacgatag
     8281 taaagtaaca atatatttta ctagtgtaat cacattaggt atcaacggct atatgaattg
     8341 cgttggccta tattagcatg gaatgcgaag cggctttatc ttactgaacg ccacactggc
     8401 gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag tgaaacataa acaacgttgg
     8461 gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt gcctttttct ggcgacgcac
     8521 atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg gcttgctgtt tttcattctt
     8581 cctggagcag ttgccagttt tttttcacag cgtagagaag tcctgaaacc tctatttggc
     8641 gcaatgctgg cggcaccctg ttcaatgctc attatgcggc tgtttttttc accgacgcgc
     8701 tcattctggc aagagctggc atggttacta agcgcggtgt tctggtgtgc gctgggggca
     8761 ctgtgtttct tatttatcag tagtttgttt aaaccacagc acagaaaaaa tcagtaaagc
     8821 cctcaacgcg agggcttgtc agacgatcag gcgtccagat tttctttcac ccatgcagca
     8881 aaatcggtat agccgccgat atgttgctga tcgacaaaaa tctgcggcac ggtttctacg
     8941 ggtttacctg ccttttgttg tagatcttct ttagtgatcc cttccgcacg aatatctaca
     9001 tactgatact gaaaatcatc gcgttcattg ctcaatttct cagccagatc ttttgcacgc
     9061 acacagtaag ggcaacccga acgaccaaaa ataacggttt gcattatttc tctcctcata
     9121 gatttatgcc tgtaatgatc acgctaaaat gtattcgctg aaagtaggtt taacctgttg
     9181 cattaattgc taaaagctat aactgttaaa cacaatacag tgaaaagttt tagactgaag
     9241 gctcactttg cagagggaag cgtatgcgcg cgatcggtaa attgcctaaa ggcgtgttga
     9301 tactggaatt tatcggaatg atgctactgg cggtggcgct gctgtcggta agcgactccc
     9361 tgtcgctgcc tgagccattt tctcggccag aagtgcagat tctgatgatt tttctcggtg
     9421 ttttgctcat gcttcccgct gcggtggtgg ttattcttca ggtggtaaaa cgtcttgccc
     9481 cacagctgat gaaccgtcca ccgcaatatt cacgttcaga aagagaaaaa gataatgacg
     9541 ccaaccattg aacttatttg tggccatcgc tccattcgcc atttcactga tgaacccatt
     9601 tccgaagcgc agtgtgaggc gattattaac agcgcccgtg cgacgtccag ttccagtttt
     9661 ttgcagtgta gtagcattat tcgcattacc gacaaagcat tacgtgaaga actggtgacg
     9721 ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt tctgggtgtt ctgtgccgac
     9781 tttaaccgcc atttacagat ctgtccggat gctcagctcg gcctggcgga acaactgttg
     9841 ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat taaccgcagc ggaatcggct
     9901 gggattgggc ggggtatata tcggcggcct gcgcaataat attgaagcgg tgacgaaact
     9961 gctgaaatta ccgcagcatg ttctgccgct gtttgggctg tgccttggct ggcctgcgga
    10021 taatccggat ctcaagccgc gtttaccggc ctccattttg gtgcatgaaa acagctatca
    10081 accgctggat aaagacgcac tggcgcagta tgacgagcaa ctggcggaat attacctcac
    10141 ccgtggcagc aataatcgct gggatacctg gagcgatcat atccgccgaa caatcattaa
    10201 agaaagccgc ccatttattc tggattattt gcacaaacag ggttgggcga cgcgctaaaa
    10261 ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt ctgacagaga ggtgcagggt
    10321 gaaaattgcc atattgtccc gggatggaac gctctattcg tgtaagcggc tgcgtgaagc
    10381 cgctatacag cgcggtcacc tggttgaaat tcttgatccg ctttcttgct acatgaacat
    10441 aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag ttaccccatt ttgacgcagt
    10501 gatcccgcgt attggcaccg ccattacctt ttatgggacg gcggcactgc gccagttcga
    10561 gatgctgggg agctatccgc tcaatgagtc ggtcgccatt gcccgggcgc gtgacaaatt
    10621 gcgttccatg caactgctgg cgcgtcaggg catcgacctg cctgtcacgg gcattgcgca
    10681 ttcgccggat gataccagcg atttaatcga catggtcggt ggtgcgccgc tggtggtcaa
    10741 gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg gagactcgtc aggcggcgga
    10801 aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt ctggtgcagg aatatatcaa
    10861 agaggcgcaa gggtgcgata tccgctgtct ggttgttggt gatgaagtgg tcgctgcgat
    10921 tgaacggcgg gcgaaagagg gctattttcg ttccaatttg catcgtggcg gcgcggcaag
    10981 cgtcgccagt atcacaccac aggagcgtga aatcgcgata aaagccgcgc gaacgatggc
    11041 gctggacgtt gctggtgtgg atattctgcg tgctaatcgc gggccgttgg tgatggaagt
    11101 gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc ggtatcgata tcgcgggtaa
    11161 aatgatccgc tggatcgaac gccacgctac gacagaatat tgcctgaaaa cgggtggtta
    11221 gtcgcaatca cattactgat catggttttg cctgcgcttt ttgcgtaagc tgtgccggtc
    11281 tttttatcga aagaggttgt acaaaattat gacatcgctg gtcgttcctg gtctggatac
    11341 gctgcgtcaa tggctcgatg gcctggggat gagttttttt gaatgtaata actgtcaggc
    11401 tctgcatctg ccccatatgc agaatttcga cggtgtcttt gatgccaaaa tcgatctgat
    11461 cgataacacg atcctgtttt ctgccatggc ggaagtccga ccttcagccg tattgccgct
    11521 ggcggcggat ttatctgcca tcaatgccag ttcgctgacc gtgaaagcat ttcttgatat
    11581 gcaggatgat aatctgccaa agctggtggt ttgccagtct ttatccgtta tgcagggcgt
    11641 aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa gagcagattt cgatggtcat
    11701 tcttgaagct aatgcccatc aactgctgtt accgactgac gatgaagggc aaaacaacgt
    11761 taccgaaaac tattttctcc actgataatt cctttcgagc acgcagtcgc tgccgcagtg
    11821 actgcgcgct gcaaaattat ctgctgtttt taaccttttc ttaaagatta tttcacttct
    11881 cttgtgtcga tttggcttta tcacatagag caaatacgca taaaaatttg ttaaataccg
    11941 ttttttaatc cgagctatag tctcaaaccc tggctaaagt tattcttgcg atgcttctat
    12001 atagcgagca gtgctggccg ggagagagtt ctcttttctt acaccgcgcc gataaaaaaa
    12061 tatgcacgtt tattgcatat ctttcagtgt gacaactttt gttcgtttgt taacgaactt
    12121 tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg ctatcgggtc tggttgcggg
    12181 tgctctgatg gccgtctctg tcggcacgct cgcggctgaa caaaaaacac tccacattta
    12241 taactggtct gattatatcg cccctgacac ggtggccaat tttgaaaaag aaaccggtat
    12301 taaagtcgtc tacgatgttt tcgactctaa cgaagtactg gaaggcaaat taatggcggg
    12361 tagcaccggc tttgacttgg tggttccatc tgccagcttt cttgaacgcc agttgactgc
    12421 gggagttttc cagccgctgg acaaaagcaa attgccggag tggaagaatc tcgacccgga
    12481 gctgctgaag ctggtcgcca aacacgatcc cgacaataaa tttgctatgc cctatatgtg
    12541 ggcgacgacc gggattggct ataacgttga taaagttaaa gctgtgctgg gcgaaaacgc
    12601 gcccgtcgat agctgggact tgatcctcaa acctgaaaat ctggaaaaac tgaaaagctg
    12661 cggtgtctct ttcctggatg cgccagaaga agtttttgct accgtgttga attatctcgg
    12721 caaagatccc aacagcacta aagcggatga ttacaccgga ccggcaaccg atctgctgtt
    12781 aaagctgcgc ccgaacattc gttatttcca ttcatctcaa tacattaacg acctggcaaa
    12841 cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc tggcaggcgt caaaccgcgc
    12901 gaaggaagcg aagaatggag tgaatgtctc gttctcgatt ccaaaagaag gggcgatggc
    12961 gttctttgat gtattcgcca tgcctgcgga tgccaaaaac aaagacgaag cctatcagtt
    13021 cctgaattac ctgctgcgcc cggatgtagt agcgcatatt tccgaccatg tgttctatgt
    13081 caacgccaat aaagcagcca cgccgctggt gagtgcggaa gtccgtgaga acccgggtat
    13141 ttatccgcct gcggatgttc gtgcgaagct gttcactctg aaagtgcagg acccgaaaat
    13201 cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc ggaaaataat ccgcagcctt
    13261 agatgccggg cgggcgcacc acacccgccg gcaattcgca ccatcatggt gcgcttgcac
    13321 acattcaatg ccggagagca gccgtgaatg acgctatccc tcgcccgcag gcgaaaaccc
    13381 gtaaggtgct gacgccgcta ttagaaatcc gcaacctcac caaatcctac gatggtcaac
    13441 atgcggtgga tgatgtcagc ctgaccatct acaaaggtga aatcttcgcg ctgctgggcg
    13501 catccggctg tggcaagtcc acgctgctgc gtatgctggc aggtttcgaa caaccttctg
    13561 ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt tccgccttac ctgcgcccca
    13621 tcaatatgat gtttcagtct tacgcgctgt ttccgcatat gaccgtggaa cagaacatcg
    13681 cttttggcct gaaacaggac aaactaccga aagcggaaat ttccagtcgg gtcaatgaga
    13741 tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa accgcatcag ctttctggtg
    13801 gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa gcgcccgaaa ctattactgc
    13861 tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga ccggatgcag cttgaagtgg
    13921 tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt cacccacgat caggaagagg
    13981 cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg gaaatttgtc cagattggcg
    14041 aaccggaaga gatctacgag catccgacta cccgctatag cgccgaattt attggctcgg
    14101 taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga tggcctggtg cttgattcgc
    14161 cggggctggt gcatccactg aaagtcgacg cggatgcctc ggtggtcgat aacgtgccgg
    14221 tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga agagccgccc gccaatggtt
    14281 gtaacttcgc ggtgggggag gtgatacaca ttgcctatct cggcgatctt tcggtgtatc
    14341 acgttcgtct gaaaagtggg cagatgatta gcgcccagct acaaaacgcc catcgtcatc
    14401 gtaaagggtt accgacctag ggcgacgaag tgcgtttgtg ctgggaagtg gacagcagtg
    14461 tggtgctgac ggtttaagga gcaaagatga atacacttga acctgctgcc cagtcgaaac
    14521 cgccgggcgg atttaagctg tggttgtcac agctgcaaat gaagcatggg cgcaaactgg
    14581 tcattgcgtt gccatatatc tggttgatct tgctgtttct gctgccattt ctgattgtct
    14641 ttaaaataag cctggcggag atggcgcgcg ctattccacc ttataccgaa ctgatggagt
    14701 gggctgacgg gcaactgtcc atcactctta atctcggtaa ttttctgcaa ctgaccgacg
    14761 atccgctcta tttcgatgct tatctccagt cgctacaggt ggctgcgatt tcgacatttt
    14821 gctgtttact gatcggctat ccgctggcgt gggcggtggc gcacagtaag ccttcgaccc
    14881 gtaatatttt attactactg gtgatcctgc cgtcgtggac ctcgtttctg atccgcgttt
    14941 atgcctggat gggaatatta aaaaacaacg gtgtgctgaa taattttctg ctgtggctgg
    15001 gggttatcga tcaaccgctg accattcttc ataccaatct ggcagtttat attggcattg
    15061 tttacgccta cgtgccgttt atggtgctgc cgatttatac cgcgttgatt cgtattgatt
    15121 attcgctggt ggaagcagcg ctggatctcg gtgcacgacc gctgaaaacg ttctttactg
    15181 tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc gatgctggtg tttatcccgg
    15241 ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc ggacagcatc atgatcgggc
    15301 gcgtgctatg gcaagagttt ttcaataacc gcgactggcc ggtggcctcg gcggtagcga
    15361 tcatcatgtt gctgctgcta attgtgccga taatgtggtt ccacaaacac cagcaaaaaa
    15421 gcgtgggaga acacggatga ataatttacc ggtagttcgt tcgccctggc ggattgtgat
    15481 tttgctgctg ggctttacct ttctctacgc gccaatgctg atgctggtta tctattcgtt
    15541 taacagctcg aagctggtga cggtgtgggc cggctggtca acgcgctggt atggtgagtt
    15601 attgcgcgat gacgcgatga tgagtgcggt tggttcaagc ctgacaattg ctgcctgtgc
    15661 ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg gtgctggtgc gctttggcag
    15721 gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg ccgctggtaa tgccagatgt
    15781 catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt gctcatgcca ttggctggcc
    15841 tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc actttctgta cggcttatgt
    15901 ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc tcgatagaag aagccgcgat
    15961 ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt acgctaccga tgatcatgcc
    16021 cgcgatcatt tctggctggt tactggcttt tactttgtcg cttgatgatc tggtgatcgc
    16081 cagctttgtt tccgggccgg gagccaccac gttaccgatg ctggtctttt ccagcgtgcg
    16141 gatgggggtg aatccggaaa tcaacgccct ggcaacattg atcctcggcg cggtcggaat
    16201 tgtcggattt atcgcctggt atctgatggc gcgcgcagaa aaacagcgga tacgcgacat
    16261 ccaacgtgca agacgtggct gaagacacta aaatttgcca acctggctac ataatgctgc
    16321 gcatgtcgcg gcattgtttt catggaagac gaaacgttgg gattttttaa gaaaacatct
    16381 tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg tggcggcgct cgccattatt
    16441 atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc tgggcgtgcg cggtattggc
    16501 gagttcattc atcgcagcgt acaaacctgg agtttaacgc tggtcttttt aagcagtctg
    16561 gtgctggttt tcattgagat ctggtgtgcg ttttcactgg tgaaagggcg tcgctgggcg
    16621 cgctggctat atctgctgac acaaatcacc gccgcaagtt acttgtgggc ggcttcgctg
    16681 gggtacggtt atccggagct gttcagcatt cccggtgaat caaaacgtga aatcttccat
    16741 agcctgatgc tgcagaagct gccggatatg ctcatcctga tgctgctgtt cgttccctcg
    16801 accagtcggc ggttcttcca gttgcaataa tgtgtataat cgtcgcccct gatgatgtga
    16861 aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc tgtcgttcct gtcagtggat
    16921 aacgcagccg attccagagc aactctccgc taaaaccgcc gatcttaaaa atctgctcgc
    16981 cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc ccggaacaag ggtttcgtaa
    17041 taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca ctgctcggta tgctgcatcg
    17101 cgatggcacg ccggaagacc tttgtgactg cccgctttat ccagcctcat ttgcgcccgt
    17161 ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta acgccctaca acgtggcgcg
    17221 taagcgtggc gaactgaaat acattctgct gactgaaagc cagagtgatg gcggcatgat
    17281 gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa ctgcgtaagg cgctgccgtg
    17341 gttacacgaa caactaccgc agctgaaagt tattaccgtc aatattcagc cggtacatat
    17401 ggcgattatg gaaggggaga cggagatcta cctgaccgaa caacaggcac tggcggagcg
    17461 ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc ttccagacta atccggcggt
    17521 cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga cagctgccgg ttaaacatat
    17581 gtgggatctg ttctgcggtg tggggggctt tggtttacac tgcgcgacgc ctgacatgca
    17641 gttaaccggg atcgaaattg cgccagaggc cattgcctgt gcaaagcagt cagccgctga
    17701 actgggctta actcgtttac aatttcaggc gctggactcc actcagtttg ccactgctca
    17761 gggggaggtg ccggagctgg tgctggttaa cccgccgcgc cgcggcattg gtaaaccgct
    17821 gtgtgattat ctttcaacga tggcaccgcg ttttattatc tactccagct gtaacgccca
    17881 aaccatggcg aaagatatcc gcgaactgcc aggttaccgc atcgaacggg tacagctttt
    17941 tgatatgttc ccgcacaccg cgcactatga agtgttgacg ctgctggtga agcaataaaa
    18001 aagccacagg tgcggcttca gattgctgat caggtgcgct ttgttcatgc cggatgcggc
    18061 gtgaacgcct tatccggcct acaaaatcat gctaaatcaa tacattgcag agttgatgtg
    18121 ggccggataa gcgcagcgca tcaggcaatc ttacttttgt catcagtctc aattactgcg
    18181 gaaaccactg gtcattgatt ttctgatatg taccgtcagc tttaattgct gccagcgcgt
    18241 tattcagttt ttccagcaga gctttgttat ccggacgtac agcgataccc aggccggtgc
    18301 cgaagtattg cggatcggtc actttttcag tcgcaacgcc cagttgcgga ttggttttca
    18361 gccattcgtt taccaccgcg gtgtcaccaa ataccccatc aatacgacca tttttcagat
    18421 cgataaaggc attctgataa ctgtcatagg agacagtttt cacttccggg tgctgatcct
    18481 gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat gcgtttgcct ttcaggtcgg
    18541 caaacgtttt gtaggtatct tttttggcaa tcacgacggc tgagttttca tagtagggtg
    18601 tggtaaacga aacctgcttg ctacgctccg gggtgatatc cataccggag attacggcgt
    18661 catattttct gaatttcagt gacgggatca ggctgtcgaa cgcgtgatta gtaaaagtac
    18721 attctgcctg catttgtttg cacaaggctt ttgccagatc gatatcaaag ccgacaatct
    18781 cattattagc acctatagat tcaaagggtg gataggtggc tgaaacgcca aaattgattt
    18841 tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa agcggcaaga actaactttt
    18901 tcatgatgga actcccgtct gtcaatctta tgatttttgg ccgtgtctgc ggcatgggat
    18961 aacaatgcca ccaagtgaat ttatatgcaa taaacatgat taaataattt aaatgaaata
    19021 aaaaaagacg gataacttag ttagttgtcc gtcttcatta taagaattta tgcactatgt
    19081 aggccggata aggcgtccac gccgcatccg gcacaggcac cgtgctgatg tctgatgcga
    19141 cgctggcgcg tcttatcaga cctacaaatc cccccggcga atgtacgcag ctacattaat
    19201 tccgccgttc gaatgccagt gctttgcgct cgatcagacg catcatcagc gtcagcaggc
    19261 cgttgacgac caggtaaata atccctgccg caccgaacac cattacatcg taggtgcgtc
    19321 cgtacaacaa ctggctgtat cccatcactt ccatcagcgt aatggtgtaa gccagagagg
    19381 tacttttgaa caccagcacc acttcgttgg aataagaaga gagcgagcgt ttaaaggcat
    19441 acggcagcag gatcgccagc gtatcttttt tgctcattcc cagggcgcta caggactgcc
    19501 actgaccttc tgggatcgca cgaattgcac cgtaaaacag ctgcgtggta tacgccgcac
    19561 tgttcagcga taacgcaatc agcgcacata accacggttc tgacaacaaa tgccacagtg
    19621 ccggatactc ctgcaacgtc ggaaactggc ccggcccgta ataaatcagg aagatctgca
    19681 ccagcagcgg cgtaccggta aacagcgtga tataaccccg caccagccac accagcaccg
    19741 gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag aatcagtgcc acaatcagcg
    19801 aggcaacggt tagcgtcagg ctggtgtgca gccctttcat cagctcgggt aaatactcaa
    19861 acattagctg ggcctccgct caaaacgtgt cgcgcgcagg tcaatgcgtt tgagaatgta
    19921 ctgactgagc agggtgatca ccaggtaaat cgccgccgcc acaatgtacc aggtaaatgg
    19981 ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt aaatcattca cactaatcaa
    20041 actgaccagc gcggtatctt tcagcagcac cagccactgg ttaccgaggc caggcagcgc
    20101 atgacgccac atctgcggca tcaccagacg gaaaaagata gccgatttcg acagccccag
    20161 cgcctgacca gattcccact ggcccaccgg tacagctttc aacgcgccgc gcagcgtttg
    20221 cgaggcatag gcggcataca gcagtgacag agcgatgaca ccacaaagga acggactcac
    20281 gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg aacccaagat tgatagtgaa
    20341 gccatccgaa agcgtcagca gtaactgcga ggagccaaaa tagataaaca gcactaccag
    20401 aatttctggc aggccacgca gaatggttac cagcgctgaa cctgcccacg cgacaggacg
    20461 ccatttcgct gactcccata ccgcaaagaa catcgccagc gccagcccga caatcaatgc
    20521 acaaacggca aggccgacgg tcatcccggc ggcgcttgct aaaggaaaaa attcattcat
    20581 caggaattac ttctggaacc atttgttgta gatggtttcg taagtgccat ctttcttcac
    20641 tttttccagc gcagtgttga gtttctgctg cagttcagtg ttgccctgac gtaccgcgat
    20701 gccgaggcca gtaccgaaat aatctttatc ggtcacttta tcgcccactg ccgccagttt
    20761 tgggttatct ttcagccact cagtgaccac tgcggtgtca ccaaatacgc tgtcgatacg
    20821 cccgttttgc agatccagtt ttgcgttctg gtagctgtca tacggaacgg tggtgatttc
    20881 cgggtgctta tccataatga acttctggtg tgtcgtcccg ttctgaacgc cgactttttt
    20941 gcctttcagc tgatcaacac tggtgtattt gccttgctga cccacaaaca gggcagagtt
    21001 gtcatagtac ggggtagtaa acagtacctg cttttcacgc tccggagtga tatccatgcc
    21061 cgccatcact gcttctacgc gacggaattt caggcttggg atcaggctgt caaacgcctg
    21121 gttagagaaa gtacaggttg catcaatctc tttacacagt gcctgtgcca ggtcgacgtc
    21181 aaaaccaacg atctggttgt ttgcatcaat cgattcaaac ggaggatagg aggcttcggt
    21241 agcaaaacga atggtttcgg cagctgtggc ggaaagacta aaacctgcaa ttaacgcggc
    21301 aatcagaact tttttcattg ttgttatccc gaatcttagt gagagagata gtttttaaat
    21361 gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt gttctacgat atgaccattt
    21421 tccatataca ccactcggct ggcggtttta cgcgccactt caacttcgtg ggtgacgatc
    21481 acctgggtaa tattcgtttc tgccagctca cgaatgatgc tgacgatttg tgccgtaatt
    21541 tccgggtcca gtgcggcggt cggttcatcg aacagcagta cctgcggttc catcatcaac
    21601 gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa gatgcagcgg gtaacgatcg
    21661 ctataaggtt tgagacgcag acgttccagc agtttttctg cacgggccag cgcctgatct
    21721 ttactcaacc ccagtacacg gcagggcgct tcaatcaggt tttgctgcac ggtcagatgc
    21781 ggccacaggt tgtattgctg aaacaccatg ccaacgttac gacgcaaatc gcgaatcgct
    21841 ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg caatgttgag cgtaccggag
    21901 cgcggcatct caagcagatt gagtacacgc agcagcgagc ttttacccgc gccgctgggg
    21961 ccaagtaaca ccagcgtttc gccctgtggg caatccagcg tgatatcgaa cagcgcctga
    22021 tgcgcgccgt agaagcaatt aatgccgttt aattgaatac tcattgacac tcgtatactg
    22081 gcagtctgat agctattgag gtcgaagata gtacctttga cagaataatt atgcaatatt
    22141 tatgctttaa aagttaaaag caaagcgcat tattcaataa acatagcaca aaataacggg
    22201 ggcggtggtc ggtgagcata aatgtcggca ttcctcacga aatgccggat aatttacgtg
    22261 gtttattggt tgatcaaggc gttagcgatt ctcgatggac tgacggagcg tacccgccgt
    22321 ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg acccaacact ggccttcctg
    22381 aatcattaac acttcatcct gccagccctg gtcaccctgt ttgagatcca cccgcaatgg
    22441 aatgttacgg gcatcacgat tagggatagt cgatgcactg gcaacgtggg cgctgtctgg
    22501 caaggtggtt cgactggaga acggatcgct gcttaacaat tcacgatgac tattatcgcg
    22561 gctggcatcg ctaagttggg ttgccagttt gtcactcaaa taggggcgca gggcggtaat
    22621 gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt tgagccacac tgtcaggccc
    22681 tccctggaca caggaaccgg tgcgggggcc gttatcttta taagctggcg tgacggtggt
    22741 acaggcactc agcagcagtg cacatggaat aagtagagat agtgaacggt agcgcataat
    22801 catttcctta ttagcttgct gtctgcgtca cactacgacc aaacgctatc agattattta
    22861 atacattgtt tttaatacaa ttctgaaaca atctgaacac ctgattgggt ttgagtccat
    22921 tgctggctac ccactcaagg ttgctcgtag ctgacttact taaattaccc aaacaataga
    22981 cagtgtaaac catttgtgag gttgagcgtg ttatatggct gaaatgatca tttaaggtaa
    23041 atatgatagt tctgaaatgg tggtataaat cctcctgtag aggttaagag catctgcaag
    23101 aagccagttt cacatttata acgcgtgata aatgaatgcg gttattgtct aatcattaca
    23161 aaatttttca catgcgataa gagatcctgc gattaagatg attgccaaaa ttacgttgta
    23221 tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac tctcgataat attatttatg
    23281 ctacaattgt gggtaaagaa tttaagctaa tgaatttgag tgaagaataa attaaattgc
    23341 ttcagtattt cgttaattaa tatgaataca aaaggtgttg tgcttttata attataaagc
    23401 gcgaggagtt cggtggatta tcgtttttct tggatgattt ttaatatgat aaacaatata
    23461 tctgatcaag catcatcatt tccaggcaca caactaaatc aaagtgataa tttcctggat
    23521 tcactgaggg aatttttcgc gatattaaat tccagtcgta agggtgagtt gtctacatgg
    23581 gataccatct acttgcatct gatattagca ataaatgctg acagtgatct cataaaaaat
    23641 gatgtgttgc tcgcggaaaa tattccatca gcgaattacc aatttaatac attttttagc
    23701 aatgctttcg agattgatgt taaaaaatat ctgggtaaat cagaagataa cgaagttgaa
    23761 ataaaagcgg gtaatgaaag aatctctatt ggaatacgta atgtatcgaa tggaaaactt
    23821 gaaagacaac agtttctttt cccactggac tatgagaata aattacaaga acaattagat
    23881 aagtatttta cgattgaatc gcacccatta ttatacaggt ataccattgg tagtaagatt
    23941 gcgaatgtga tttttgaaaa gttatacagt cgtattgact ttaacaaaga acaatatata
    24001 tcctttataa aggacgcatt tattcatttt tatgattata gcaggcgata tgctattagt
    24061 gagaatatcg ataaagacgc tgtaacaaac aatatcgcat tgatgagcac tttttatgac
    24121 agtgataata cgtcaggtga ggttttaaat aatgatttta cagaagaaga gtcatttgaa
    24181 actgcgttag atgtagagca tgcaatagtg cttggatttg ctgatgataa ttttgaaaca
    24241 aaacctgttc attatcagga tttattaaca cgatttagtg cctttcaaga taccgtgttt
    24301 aatctgtttc ctgaaatgca ctcttcgcat taccacgata tttgtagcgt aagtgtggat
    24361 atgacaaagg gaacgcaatg catgattcac ttgatggtga atgaagaagt tttcatgtct
    24421 ctgcctgttc cagttgccac gatggtacgt gaagatgcaa gtaatttagt caatcttaaa
    24481 acactactca atgatggttg tttcattaaa tatagtcatt ttaatgacgt ggcattaata
    24541 aagcaaaaca taagtaatct ctatttatca cacactgttg tgaatgaaag catattgaaa
    24601 aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa taacaaatag caatgtaata
    24661 aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa ttaataacgt aaatactcat
    24721 tctttaaaat ttattaactg caattttttc aatgttgata tgataagagt taatctcagc
    24781 aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga taaaaccctg gttgcctgta
    24841 aaatggacaa aagaattaat aagtgattat ttgtatggct gtttactatc tctgtactct
    24901 atctgtgcaa gggatatata taatatgaac gccgggaata acgtgaaggt tgctgcggat
    24961 gctttccttg aaattatttt ctctcttaaa aataaatatt gtattaaact attaagcgct
    25021 caggatcggg cgtttatata cgagtttgca agaatgatct tcgcttatat taatgacaaa
    25081 agcattgaga tcttattact ttcttgtttt gcggcggctg accaaaaagc aatccagcgg
    25141 tatagacctc agtcacaaga tggggaagac tttaggagtc atttacaata taagttgcct
    25201 ctttccgctc attaatgttg ttggtgcaac ataataagac aggtaagcgg taccctataa
    25261 tgaaaccaat atcgggttat gcaacggata atcgtaaaga cttaagtggg tgtttttgac
    25321 acaatgagat gatttcctaa actgatcaaa accttcttcc caacatggca tatcagtccg
    25381 ataatgcttt agagatcgaa ggcttaacaa acaaggagat cgatcatgca attttctaca
    25441 accccaactc tggaaggcca gaccatcgtt gaatattgcg gtgtggtgac cggcgaagcg
    25501 attttaggtg ccaatatttt ccgtgatttc tttgccggta tccgcgatat cgttggcgga
    25561 cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg agatcgcctt tgaggaatta
    25621 ggctcccagg cgcgggcgct gggggccgat gccgtagttg gtattgatat cgactacgaa
    25681 acggtcgggc aaaacggcag tatgctgatg gttagcgtca gcggtacggc ggtgaaaacg
    25741 cgtcgatgag aagagtcttc tggctggtcg ctgccgctct gttactggca gggtgtgcag
    25801 gcgaaaaagg cattgtcgag aaagagggat atcagcttga tacccgacac caggcgcagg
    25861 cggcgtatcc gcgcattaaa gtgctggtga tccactacac cgcagatgat tttgatagct
    25921 cgctggcgac actgactgat aagcaggtca gctcgcatta tctgatccct gcggtgccac
    25981 cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc ggaacaagaa ctggcctggc
    26041 atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa cgacacctct attggcattg
    26101 agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa atattttgcc ccgtttgaac
    26161 aggcacagat tcaggcgctt attccgctgg cgaaagatat tattgcccgt tatcacatca
    26221 agccggaaaa cgtagtggca catgcggata tcgcaccgca gcgcaaagac gatccggggc
    26281 cattatttcc ctggcagcaa ctggcgcagc aggggattgg tgcctggccg gatgcgcagc
    26341 gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc ggtagatacc gcgtcattgc
    26401 tggagctttt ggcgcgctac ggttatgacg taaaacctga tatgacaccg cgcgagcagc
    26461 ggcgcgtgat tatggcattc cagatgcatt tccgcccgac gttatataac ggcgaagcgg
    26521 atgcagaaac tcaggcgatt gccgaagcat tgctggagaa atacgggcag gattagcgcg
    26581 gcagttttcc gtggtcgcgt agccaggcgg cagttttctc gatgccttca tccagggtga
    26641 tgaccggctg ataacctaac tcttcctgcg cacgcgtaat atccagcgta aagtcaaaat
    26701 tcaacttgga gacgccgtag tgggtcagcg gcggctcttt tgctgacttg cggcctaaac
    26761 gctccatgct gtgggcgatc atatccagca tcgggtaagg gacggaacga atacgacagt
    26821 caatattcaa ctcgtcgatc agcttctgca cgatgctgcg cagtgtgcga tgctcgccgt
    26881 tggtgatgtt gtacacacgc ccggaaggta gcttatcgca ggcttcctgg cttgccagcc
    26941 acattgcgtg cacggcattt tcatagtagg tcatatcgac cagcgcactg ccgccatgcg
    27001 gtaacagaat actgccgtag tggtgcatca tatgcgccag acggggaata aagactttat
    27061 cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa gcgcgtttgt ggattcgcct
    27121 gcgaaagcat attgatcact tcttcgctgg ctgctttgct gcgggcaaac tcgttggcga
    27181 agcggtgagg gcgaaaatct tctttaatat cgcgatggtg gtgataatcg aagtacaggg
    27241 agggggaaga gatatgaata aagttacgca caccccaggc gacagcccat tcacccaggc
    27301 ggcgagtggc gcgaacgtta gccagatcga aagcctgttg tgtcccccag ggtgaggtaa
    27361 agctggagca gtgccacagc gtatcaatgc ccgcgagcat cactttagct tgtgatgaaa
    27421 ccagctcggt cagatccgtc agaacaaact ctgcgcccat tttttccagc aatttaccca
    27481 ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt ctggcataaa aactctaccg
    27541 cgtttcgacc taagccgctg gtggcgccgg taaccagtac cttcatatca atccactgtt
    27601 gttgagaaaa tgacgtgcgc attcttccgt gatttccccc atgatgcaat gggaaacatg
    27661 aaagaataac gcaggttttg tcgattaatc tgtgctttgt tctgccagtc tggcgatttg
    27721 ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc atcaataacc agtaaaacag
    27781 ccccggcata ccgtgcggat gccagaaagc gcggacatcg atagtacgat agtcaccttt
    27841 atcttccagg gtaaaacaca gtcgtcccag ccccggcgct ttcatgccaa ataacaacgt
    27901 aagttgcttt ttcggttcaa cgacaatcac tttccagcta tccaccgcat cgccggtctg
    27961 taaatattcg cgctccgggc ggcctttcgc cagattatga ccgatcgcgc ggtccatcaa
    28021 cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc tctttaccgc cgatttggtt
    28081 caccaccagc cataaagccg caaggctggc ggacgtttta acggtaaacc ccgcctgttt
    28141 ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc tgagcgtcgt agccccagtc
    28201 gctggagttg accagttttt cttcctcttt caacgtgcta cgtaccgcgt catcgaaagc
    28261 gatcagccgt tgtgggatga gtgcacgtag cgcggtatca tctgccagca gatcgtgttt
    28321 cagcccctga atcaacgccc tggcggtggt gggcggtacg gaagtaatca cattgagaaa
    28381 ccacaccgaa atccagcggg tggggagggg gatggggatc aaccagcggc gcttaccgct
    28441 caccgccata aaatgttcaa actgttgctg ataactgagc acctctggtc cggcggcttc
    28501 gaagatgcgg tgtttgttgg ctggatggtc taacagcgcc accagatagt gcagcaagtt
    28561 ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt ggcggcgtta acactggcag
    28621 gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa cctgcgccaa cgataattcc
    28681 ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga atgtccgccg tagcctgacg
    28741 agctcgcaga tgatccgact gctcatgtgg cggggcctgc aacgaactga gaaagattaa
    28801 ttgcttaact ggtacttcac gtagcgcatc gcggacgttg agagccacct ggcgctcctg
    28861 agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga aaatagacgg tatcgatatc
    28921 ctgcaacagg gcaggaaggt tatccggcca gctgagatcg actttatggc aactgacgtt
    28981 tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc gccgccagga tctgatgccc
    29041 ttgctggctg agtgtgcgca ccagatgctg accaatgtag ccactggcac cgagaactaa
    29101 aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa tgcgcgccag tgggcggcga
    29161 cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac caggcggaca atcggcgagg
    29221 cgttaatcag cacgtttctc gctttcatgt attcgcctaa cgcggcagca ttttcttccc
    29281 cgacgcgaac aaacagcata ttagtgtcct gacgcatcac atccgcgcct gcttcacgca
    29341 gttgctccgc catccaggcg gcgttgtcgt gatcttcctg caaccgtgcg acgttatttt
    29401 tcagggcata catcccggcg gcagccagaa tcccggactg acgcatcccg ccaccggcca
    29461 ttttccgcca gcggatggca cgtttaatgt aatcacgatt accgacgagt aatgaaccga
    29521 ctggcgtccc aagacctttc gacaggcaaa tggtgaacga atcacaatat tgcgtgatct
    29581 ctttcagttc gcagccgtaa gccaccacgg cattaaagat gtgcgcaccg tcaacatgca
    29641 gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt caggtattca cgcggcagca
    29701 ctttgccgtt gtgggtgttt tccagactga gtaatttggt gcgggcgaaa tggatatcgt
    29761 cgggtttgat tttcatcgcc actttatcca gcggtagcgt gccgtcggca gccgcgtcga
    29821 tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc ggcttcaaac agatagttat
    29881 gcgcggcctg accgacaata tactcttcgc cacgttcgca gtgactgagc agagcgacca
    29941 ggttggcctg agtgccggta ggcagaaaaa tggcggcttc tttaccggaa agctctgctg
    30001 catagtcctg cagagcatta acggtagggt cgtctccgta aacgtcgtcc ccaaccgggg
    30061 cggccatcat cgcttcgagc atggcgcggc tcggtcgggt aacggtatca ctgcgtaaat
    30121 caatcatggc atgtccttat tatgacggga aatgccaccc tttttacctt agccagttcg
    30181 ttttcgccag ttcgatcact tcatcgccgc gtccgctgat aattgcgcgc aacatataca
    30241 ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg aatggctaat tcttctttgg
    30301 cgaccaccac atccaccaac accggaccgt cgatagagaa ggcgcgttgc agggcttcat
    30361 caacttcaga ggctttttct acacggatac ccgtaatgcc gcacgcttcg gcaatgcggg
    30421 caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta acctccggcc ttcatctcca
    30481 tcgccacaaa gcccagcacg ctgttattaa agacgataat tttcaccggc agtttcatct
    30541 gcactactga gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg cacatggcga
    30601 ccacctgacg ttccggctcg gttgcctgcg cacccaacgc ctgcggcatg gcgttagcca
    30661 tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc gttcattttc agataacgtg
    30721 ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat ggcgtcatcg gcggcaaaat
    30781 gactaatttg ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt ttagctaaat
    30841 cgtccagccc tttgcgggca tcgcggtaat cttccagcgc tttatccaga aacttgcgat
    30901 cggttttttc ttccaccagt ggcagcaatg cgcgcagggt tgatttgata tcgccgacca
    30961 gcgccatatc taccttgcta tgtgcgccga tgctggctgg attaatatca atctgaataa
    31021 ttttggcatc ggtcgggtag aacgcacggt aggggaactg agtaccgagt agcactaacg
    31081 tgtcggcgtt catcatggta tggaaacctg acgagaatcc gattaacccg gtcattccaa
    31141 catcatacgg attatcgtat tcgacatgct ctttaccgcg tagggcatga acaataggtg
    31201 ctttaatttt cccggcaaac tcaactaact ctttatgcgc ccccgcgcag ccgctgccac
    31261 acatcagggc gatattgctg gaataacgca gcagttgcgc cagtttgcgt aactcttctt
    31321 cttccggcgt cacgactggc tgtggcgcat gataccagtg cgtggttgcc ccttctggcg
    31381 caggttttaa cgccacgtcg cccggtaaca caacaacgga aacgccacgg ttaagcaccg
    31441 ctttgcgcat agcaattgcc agcacttgtg ggatctgctc cgggctggaa accagctcgc
    31501 aatagtgact acattcgcgg aatagctctt gtgggtgggt ttcctggaaa tagccgctgc
    31561 caatttcgct ggagggaata tgagcggcaa tcgccagtac cggaacgtta ttgcggtggc
    31621 aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc gcacgatccg gcacagaccg
    31681 ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa cgccgccact tcttcatggc
    31741 gggtggacat ccactcgatg gtgcccatgc gattaagact gtcgctaaga ccgttcagag
    31801 agtcgcccgt gactccccag atgcgtttca cgcctgccga ttcgagtgtt ttggcgatat
    31861 aagctgcaac cgtttgtttc atggttctcc atctcctgaa tgtgataacg gtaacaagtt
    31921 tagttcatct gacggagggg gaagggatgg gagagaaagg aggcactaac ggttaaatag
    31981 cccgatgaaa ggaatatcat cgggcataag gcgattatgc gagaaccaaa tccccctgcg
    32041 gatggcagga gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc agcgtcattg
    32101 tactgctcac cgtatattca ccggaaacca cttttgtctt acagcagccg caaacacccg
    32161 cacggcaggc agcgacaacc ggaacgttat tgctttccag cgcctccagt agcgtggtgc
    32221 caaccggggc gtaaaattct cgtgccggtt gcagtttggt gaatttcaga ccgctggtcg
    32281 ccgcttctgc tactggggtg aagaatttct ctttaaagaa acgcgtcacg ccgagcgctt
    32341 tcacttcctg ctctacccaa tccatatacg gagccgggcc gcaggtcatc acggtacgtg
    32401 aagctaagtc aggtacatct gccagcagtt cgcgagtgag acgaccagcg ataaagcctt
    32461 cggtaacgtt attttctgcc accagcgtta ccggatagtt acgccactca tcggcgaaaa
    32521 taacatcctg cggcgtacgc acgttgtaga tcacccgcac atcggcctgt ggacggttct
    32581 tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc gcagcctgcc gccagcaaca
    32641 ggaatttatc ttctgcttta tcgtcgcagg taaattcccc catcgcgtcc gaaagccaga
    32701 gataatcacc gcgttttaca tcgcgcgtca gccactggga gccgacaccg tcatcaatcc
    32761 gccgcacggt cagggtgata tattcactca cgcctggcgt ggaggaaatg gtgtaagcac
    32821 gcagcgtttc cgctgagttt cgcacgctga ccagtgcata ttgcccggcg cgatatgggt
    32881 agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc gtaatgtgat
    32941 gaacctgcat ccgccacggg cattgattcg ttggcatcgt catcgacaaa ctccttacgc
    33001 gctcaacagt tgcttcatgt cttcttcaac agtggtgata gaacgcaggc cgaatttctc
    33061 gttcagcacc gccagcaggt ctggtgtcag gaaactaggt gcagtcgggc cggtgacgat
    33121 atttttcacg cccagagaaa gcagcgtcag cagaatgaca atcgctttct gttcaaacca
    33181 ggagagcacc agcgacagcg gcagatcgtt cacaccacag cccagtttct ctgccagagt
    33241 gacagccaga ataattgctg agtaagcatc gttacattga cctgcatcta ccagacgcgg
    33301 cagaccttcg atatcgccaa actcaagttt gttaaagcga tatttaccac aggcgagggt
    33361 caggatcagg cagtcatccg gcacgctggt ggcgaaatcg gtgaagtagt ggcgctcgcc
    33421 gcgtgcgccg tcacagccac caagcaggaa gatatgacgc agtttttcac ggctaaccag
    33481 atcaatcagc gtatcggctg caccaagcag cgtctggcga ccaaagccca cggtgatcag
    33541 gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc gcctgggcga tgaccgcaga
    33601 gaaatcttcg ccatccagat gacgcacgcc aggccagcca acgatgctgc gggtccagat
    33661 acgatcgtca taagcgccta cggttgggtc gatgatgcag ttcgaggtca tcacgatggg
    33721 gccagggaaa cgagcgaact ccacttgctg attctgccag ccgctgccgt agttaccgac
    33781 cagatgcttg aatttacgca gctccgggta gccatgcgca ggcagcattt cgccgtgggt
    33841 gtagacatta acgcccgtgc cttcggtctg ttccagcagg ttgtagagat ctttgagatc
    33901 gtgaccggaa atcagaatgc atttacccgc cgtcgctttg acgttgacct gggttggcgt
    33961 cgggtgaccg tatttaccgg tttcgcctgc atccagaatg ctcatcactt tgaagttcat
    34021 ctggccgatt tccattgaac actcaagaag cgcgttcata tcggcaggcc aggtccccag
    34081 ccacgccatg attttatggt actgggcata aatatcgttg tcgtattgac cgagaacatg
    34141 cgcgtgttcc atataggccg ccgcaccttt caggccatac aggcacagca gacgcaggcc
    34201 gagaatgttt tcgccaatcg ccgctttatc tttgttaggg gtaaattctg ctgcctgacg
    34261 ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca gccatcgggt tatcgacgcg
    34321 cgcgttggca tctacagcca ggcattgtgc tttcagcgcc tcgcgcaagg caatcgcttc
    34381 acgagcgtag ccgacaatac gcggagaatc gaagttaacg ttggtcaggg ttgagaaaaa
    34441 ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg ccgtattcac gcgcttttac
    34501 cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt cagaagtttc
    34561 cgccgtttta ccacacatcc cctgcgcgta tgagcagccg tttcctgccg gagtacggat
    34621 agtttgttca cattgcacac aaaacatgat cacacctttt aaagttatat ttaatataca
    34681 tgtttaaggt taagacgctt aacgcgggga taaaagggat ttttcatgca actttaaggg
    34741 agattgattt agcgcaattt tggcggcagg gatctaccgc cagagaggta ttacgcagag
    34801 aaaaaggcga tgaggatcgg cactaacagg ctaagaataa aaccgtgaac aattgccgcc
    34861 gggaccatat ccagcccgcc agtacgttga agaacgggca gggtgaaatc cattgatgtg
    34921 gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa tcagcccagg gatcaacata
    34981 atagcaatca gttcacgggc cagatcatta aaaaacgccg cgctcccgat taccggacca
    35041 aaagattcgg tcaataaaat accggaaaga gaataccagc cgaaaccgga ggccattgcc
    35101 agcgcggtat tgatggggag atcaagaata aaggcgttaa ttaaaccacc aattaatgaa
    35161 ctgacaacca ccaccacggc gacaatcatt ccccggcgat taaggacaat ctgctttaag
    35221 gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa gtagcaaaat taacgtgtat
    35281 tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac tcagaccaat ggcaaaacca
    35341 atcactacta cgccgcacag ttttagcgac tccagcgcca tcgcaatacg cgacgggagt
    35401 ttttcttgct gatggtggtt gcgccacggc aggcctcgct ccagccacat cagggcggca
    35461 atattacaca gtaaaataac ggtaatactg acggcagaat aatgcagaat cgccaacagg
    35521 ttactggcga ggttatcgag aaacgccaga ctgataccca taaaaaagag aataaggtaa
    35581 accatccagc ttaatagctg attaataact tttaacgcag cttgttggcg aagcggaatg
    35641 aggtaaccca caatcagggg aaccagaatg attaacagcc cagaaaacat gaaaatccag
    35701 tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta ctgaaagcgg aaggacgagt
    35761 aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg atttttaatc ctatctgagt
    35821 gtttagcatt gttgcgctcc tttcatgtgc aatgccatat aaagccactg gcggagggag
    35881 cgcgcagggg gcagccagtc gccgccgccc cctgcacccc cgggctctgg cgaacaaaat
    35941 cgccgctgcg cggtaccttc agcttatccc cttcggcaac caggtcgggc gcgaggtaac
    36001 gtcctgtaag acgcgccctc agcccacatc catgtgggct gccccggcct tcggggaacg
    36061 cttcagcgat tttgacgcca ccaatacccg tgcggtttgt aatgtcgaag ataaaacaac
    36121 cagggcagat aaaatgccaa tgctaattaa tcacgctttt ccagcagagt ccggtaaatc
    36181 agaccaccga taatgccgcc gacaattggc accacccaga agaaccacag ttgttctaat
    36241 gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg ccgggttaac agaagtgtta
    36301 gtcaccggaa tactgattaa gtgaatcagg gttaaggcca gaccaatagc gatcggcgca
    36361 aaacctgccg gagcgaattt gtcggttgca ccgtggatca ctaacaggaa gcccgcactc
    36421 aataccagtt caactaccag cgcggaaagc atggaataac cgcctggtga atgctcgcca
    36481 taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac ccgttttacc actgacaatt
    36541 aaatacagca gcgccgctgc aacaataccg ccgacaacct gggcaatcac gtagccaacg
    36601 acttcttttg ccggaaaacg tccgccagcc cataaaccaa tagtgaccgc cgggttaaaa
    36661 tgaccaccag aaatatgacc aacagcgaag gccatcgtca gaacggtcaa accaaatgcc
    36721 agcgccacgc cggcaaaacc aatgcctaat tccgggaagc ctgcggccag tacagcacta
    36781 ccacagccac caaaaacaag ccagaaagta ccaaaacatt cagctgctaa ttttctgaac
    36841 atatccacct caattaaaaa ttgaccctgt gaaaaatagg gtcgttttat agggtcgtcg
    36901 taaaaaatga cgacggaaat aatgcgcggc tattttaaaa acgaaggcga gtcattcacc
    36961 agataaataa atccagtaaa tttgatttag ggcaacagcg ggttgcccca tatagtcatt
    37021 tgtctgattg acagtgtagt gcaagcaaaa gatttaatcc tttaggcgta ataaaaaata
    37081 atttatcatg ctaattattt gattttgttg tttttgcggg cttatcagca agagggagta
    37141 taacgcgatt attcgctcat ttttcagaca attgccatgc ttaaatgtga tgtcatcacg
    37201 tattagcaag gcctttcccg ttatactgcc agcgtaaagg ataagtcaca tatttctgga
    37261 ggggatatga ttcttgagcg cgttgaaatt gtgggttttc gcggtatcaa ccgtttgtcg
    37321 ttgatgctcg aacaaaacaa cgtcctgatt ggggagaacg cgtggggtaa atccagcttg
    37381 ctggacgcct taaccctgct gctatcgcca gaatcagatc tctaccattt tgagcgcgac
    37441 gatttctggt tcccgccggg agatatcaac gggcgagaac atcatctgca tattattttg
    37501 accttccgcg aatcgctgcc aggccgacat cgggttcgcc gttatcggcc gctggaagcg
    37561 tgctggacgc catgcaccga tggctatcac cgtatttttt atcgtctgga aggggagagt
    37621 gcggaagacg gcagcgtgat gacactgcgc agttttctcg ataaagacgg acatccgatt
    37681 gatgttgagg atattaacga tcaggcacgc catctggtgc gtttaatgcc ggtgctgcgc
    37741 ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca cggtgccaaa tgtccccaat
    37801 gtggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg agttgtcctc acatccgcaa
    37861 aatctctctg atggacagat tcgtcaggga ctttccgcaa tggtgcagtt gcttgagcat
    37921 tatttctctg agcagggggc tggacaggcg cgatatcgtt taatgcggcg gcgggccagc
    37981 aatgagcaac gaagctggcg ctatctggac atcatcaacc ggatgattga ccgacctgga
    38041 gggcgctcgt atcgggttat tttgctcggc ctgtttgcta ctttattgca ggcaaaaggc
    38101 acattgcgac tggataaaga cgcgcgtccg ttattgctaa ttgaagatcc agaaacccgc
    38161 ctgcatccga ttatgctttc cgttgcctgg catctgttga atcttttgcc tttacagcgt
    38221 attgctacca ccaactctgg cgagctgctt tcgttaactc cagtggaaca tgtttgccgc
    38281 ctggtgcgtg aatcttctcg tgttgccgcc tggcgtctgg ggccaagtgg cttgagcacc
    38341 gaagacagcc gccgcatctc ttttcacatt cgttttaatc gtccgtcatc gctgtttgct
    38401 cgctgctggt tgctggtgga aggggaaacg gaaacctggg ttatcaatga actggcgcgt
    38461 cagtgcggac atcattttga tgccgaaggg atcaaggtca ttgagtttgc ccagtccggg
    38521 ctaaagccac tggttaaatt tgcccgccga atggggattg aatggcatgt actggtcgat
    38581 ggcgatgaag cagggaagaa atatgccgct acggtacgca gcctgttgaa taacgatcgg
    38641 gaagccgaac gagaacattt aacggcgtta ccggcgctgg atatggaaca ttttatgtat
    38701 cgccagggat tttccgatgt gttccaccgc gtggcgcaaa tcccggaaaa tgtaccgatg
    38761 aatctacgca aaattatctc gaaagcgatc catcgctctt ccaaacccga tcttgccatt
    38821 gaagtggcaa tggaggcagg acgtcgtggt gtggactccg taccaacgct gctgaaaaaa
    38881 atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg attaaccgcg aaacatcgtg
    38941 gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc gacggtattc agcccgtttt
    39001 ttactggcga tttcggcaat ctcttttcgt gctatctgtg ctggaaggcg gtaatggcgt
    39061 tcagcatcac atacgccgcc aaccgactcc cagaacgcgt tgtaatcagc atggatcttg
    39121 ccttctttat cgcgataacg caggctgcgg taaatatgcg tttcattgct gacggcaata
    39181 atctgctcta cctgcaaacg ttgggcaaac agacaggccg cttccatcac gaggcgtttg
    39241 ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt cctgatgtgg aatttcccat
    39301 tttgcgcctt gcagtccgcc aataaacatc gttcttttcc cctgatattc acacagggta
    39361 aacgtgatct ctgccagagg aataccttcg ctgttgcgga acaggattgt gctgtcacct
    39421 tctttatcca ttgagatcat catggtcagc tcaagcgtga actgctcacc gtttttgcct
    39481 tccagtttag ccagttgcag cccgggggta ttcaaatata agctgaattc ttccgccgac
    39541 atacacccgc ggagtaacgc ataatggtaa cgtaacgcct ccagcaattg cttacggcta
    39601 agattcgccg caaggtaagg gcgatgcaga cgcacaggca gtcgcggctg gcgcgttaac
    39661 aacacattga gattaggcca gtgggaaagt tcgttcatcc actcaacgct taaacgcggc
    39721 ataatcaacg agcgcagcaa aaatttctgg cgaaaactac agcgatgcca gaatttaccc
    39781 ggccgacact gtccacgtgc cagactaaga aaaagtgaca ggctgctgag agattcagat
    39841 ggcgtaaagg tccgttcagt tagctgcgac atattcatga aatcaatggt tatacatgac
    39901 gtcgatttca ccattgcgta tcttaaccaa acatcaatag tgtgattact aacgtaaatt
    39961 ttagggtttt gttgatattt cgttgaagtt aatgacccgg attggcatgt ggagtattca
    40021 gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta cgttattgcg ctggtgatag
    40081 tcatcgccgg actgattacg ttatggagaa ttcttaacgc acccgtgccg acttatcaga
    40141 cactgattgt gcgccccggt gatttacagc aaagcgtgct ggcgaccgga aagctggacg
    40201 cgctgcgtaa ggttgacgta ggcgcgcagg tcagcggtca gttgaaaacg ctgtcggtgg
    40261 cgattggcga taaagtaaaa aaagaccagc ttttaggggt cattgatcct gaacaggctg
    40321 aaaaccagat caaggaggtc gaagcaacgc tgatggagct acgcgcgcag cggcagcagg
    40381 cggaagcgga gctgaaactg gcgcgggtga cgtattcccg tcagcaacgt ctggcacaaa
    40441 cgcaagcggt ttcactgcag gatctcgata ccgccgcgac ggagatggct gtgaaacagg
    40501 cgcaaattgg caccattgac gcgcaaatca agcgcaatca ggcctctctc gatacggcta
    40561 aaaccaatct cgattacacc cgtatcgttg ccccgatggg cggggaagtg acgcaaatca
    40621 ccactctgca aggccagacg gtgattgccg cacagcaagc accgaacatt ctgacgctgg
    40681 cagatatgag cactatgctg gtaaaagcgc aggtttctga agcggatgta atccacctga
    40741 agccggggca aaaagcctgg tttacggtgc ttggcgatcc actgacgcgc tacgaggggc
    40801 aaatcaagga tgtgctaccg acgccggaaa aggtaaacga cgctattttc tattacgccc
    40861 gttttgaagt acccaacccc aatggtttgc tgcggctgga tatgaccgcg caagtgcata
    40921 ttcagctcac cgatgtgaaa aatgtgttga cgatccctct gtcggcgtta ggcgatccgg
    40981 ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg tgaaacacgc gagcgtgaag
    41041 tgacgattgg cgcacgtaac gataccgatg ttgagattgt caaagggctg gaagcgggcg
    41101 atgaagtggt gattggtgag gccaaaccag gagctgcaca atgacgcctt tgctcgaatt
    41161 aaaggatatt cgtcgcagct atcctgccgg tgatgagcag gttgaggtgc tgaagggcat
    41221 cagcctcaat atttatgcgg gtgagatggt cgcgattgtt ggcgcttcgg gttccggtaa
    41281 atcgaccctg atgaatattc tcggctgcct ggataaggcc accagcggca cctatcgcgt
    41341 agccggtcag gatattgcca cgctggacgc cgatgcgctg gcgcaactgc gccgcgagca
    41401 tttcggcttt attttccagc gttaccattt gctttcgcat ttaaccgccg agcagaacgt
    41461 tgaagtaccc gccgtctatg ccggtcttga gcggaaacag cgactgcttc gtgcccagga
    41521 gttgctgcaa cggctggggc tggaagaccg tacagagtat tatccggcac agctttcggg
    41581 tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg aacggcggtc aggtaattct
    41641 tgccgatgaa ccgaccggcg cactggacag ccattctggc gaagaggtga tggcgatcct
    41701 gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc acccacgatc cgcaggtcgc
    41761 tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa attgtgcgca atcctcccgc
    41821 cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt gtcaatacag tgtctggctg
    41881 gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg gcatggcggg cgctggcagc
    41941 gaataaaatg cgtactttac tgaccatgct ggggattatt atcggtattg cgtcggtggt
    42001 ttccattgtc gtggtgggtg acgccgccaa acaaatggtg ctggcggata ttcgttctat
    42061 tggtacgaat actattgatg tctatcccgg gaaagatttt ggcgatgacg atccgcaata
    42121 tcagcaggcg ctgaagtacg acgacttaat cgccatccaa aaacaaccgt gggtcgcctc
    42181 agccacacct gccgtctcgc aaaacctgcg cctgcgttat aacaatgttg atgttgctgc
    42241 cagtgccaat ggcgtgagcg gcgattattt taatgtctat ggcatgacct tcagtgaagg
    42301 aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag gtcgtggttc tcgacagtaa
    42361 tactcgccgc cagcttttcc cccataaagc agatgtggtt ggcgaggtga ttctggtcgg
    42421 caatatgccc gccagagtca ttggtgtggc ggaagaaaaa cagtcgatgt ttggtagcag
    42481 taaagtgctg cgtgtctggc taccttacag cacgatgtcc gggcgagtta tgggccagtc
    42541 gtggcttaac tccattactg tcagggtgaa agaaggattt gacagcgccg aggcggaaca
    42601 gcaactcacg cgtttacttt cactgcgcca cggtaagaag gatttcttta cctggaacat
    42661 ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact ttacaactgt ttctgacgct
    42721 ggtggcggtg atttcgctgg tggtgggcgg tattggtgta atgaatatta tgctggtgtc
    42781 agtgaccgag cggacgcggg aaattggcat tcgcatggct gtaggtgcgc gagcaagcga
    42841 tgttttgcaa cagttcctga tcgaagccgt actggtttgc ctggtcggtg gcgcgttggg
    42901 aataacactg tcactgttaa ttgctttcac cttgcagctt ttcttacccg gctgggagat
    42961 tggtttttca ccgttggcgc tgctgctggc gtttctctgc tcgacggcca ccgggatttt
    43021 atttggctgg ttacccgcac gaaatgcggc acgactggat ccagtagatg ctctggcacg
    43081 ggagtaattt ttgagataaa aatgccagcc gatcgggctg gcattttgcc tttaggatgt
    43141 acacaatgag acagaagagc tatgcgactg ccgcttctac ttcgacgggc acaataacac
    43201 tggcgtgatt gccttttggc ccctggtgga catcaaactg aacggattgt ccagctttta
    43261 gcgttctgta accatccatc tgaatggtgg aataatgagc gaaaatatct tcgccgccgc
    43321 cttcagggca gatgaaacca aaacctttgg cattgttgaa ccacttaaca gtaccctttt
    43381 ccatgcttcg acatccttcg caaatcttat acaagtaaga tggaataaac cggggtcaga
    43441 gagggggctg ttcaaaacct cgccaactct agaaatacaa tttagagaat tagggcgagc
    43501 cgtcaagcat ttgacagggg ccagggggca ggtatgaatc aaaaatttga agcagttaac
    43561 gctattgaca ggaatgtgac agatgtcgct gatgccaacg atagatgata gttatctatc
    43621 atgtggagta gattggtcag gcaaataagc tcttgtcagc ggcagggcgt tctgccgata
    43681 accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa cgactggctg gactttgatc
    43741 aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc atctatgtat aaagtgatat
    43801 tagtcaatga tgattacact ccgatggagt ttgttattga cgtgttacaa aaattctttt
    43861 cttatgatgt agaacgtgca acgcaattga tgctcgctgt tcactaccag gggaaggcca
    43921 tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt ggcgatggtg aacaagtacg
    43981 cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa agcctgaatg caggcataaa
    44041 aattggggga ggtgcctatg ctcaatcaag aactggaact cagtttaaat atggctttcg
    44101 ccagagcgcg cgagcaccgt catgagttta tgaccgtcga gcacttgtta ctggcgctgc
    44161 tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc tgtggatttg gttgcgctcc
    44221 gtcaggaact ggaagccttt attgaacaaa ccacacccgt tctgcctgcc agtgaagagg
    44281 agcgcgacac acagccgacg ctgagttttc agcgtgtact gcaacgtgcg gtcttccatg
    44341 tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt tctggtcgct atcttcagcg
    44401 aacaggagtc gcaggcggca tatctgttgc gtaaacatga agtcagccgt ctcgatgtgg
    44461 tgaactttat ctctcatggc acgcgtaaag acgagccgac acagtcttct gatcctggca
    44521 gccagccaaa cagcgaagaa caagctggtg gggaggaacg tatggagaat ttcacgacga
    44581 acctgaacca gcttgcgcgt gtgggcggaa tcgacccact aattggtcgt gagaaagagc
    44641 tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa caacccgctg ctggtggggg
    44701 aatctggtgt cggtaaaacc gcgattgcag aaggtcttgc ctggcgaatt gttcagggtg
    44761 atgtgccgga agtgatggct gactgtacga tttactctct cgatatcggt tctctgttag
    44821 cgggcacaaa atatcgcggc gactttgaaa aacgttttaa agcgttgctc aagcagctgg
    44881 agcaggacac taacagcatc ctgtttattg atgagatcca caccattatc ggtgcgggtg
    44941 cagcgtctgg tggtcaggtc gatgcggcta acctaatcaa accgttgctc tccagcggta
    45001 aaattcgcgt aattggttcg acaacctatc aggagttcag caacattttc gagaaagacc
    45061 gtgctctggc gcgtcgcttc cagaaaattg atattactga accgtcgatc gaagaaactg
    45121 ttcaaatcat caatggcctg aaaccgaagt atgaagcgca ccacgacgtg cgttataccg
    45181 caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata cattaacgat cgtcatctgc
    45241 cggataaagc cattgatgtt atcgacgaag cgggcgctcg cgcacgtctg atgccggtaa
    45301 gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc cgtggtggcc cgtattgcac
    45361 gcattccaga gaagagtgtt tctcagagtg accgcgatac cctgaaaaac ctcggcgatc
    45421 gcctgaaaat gctggtcttc ggccaggata aagccattga ggcgctgact gaagccatta
    45481 agatggcgcg tgcaggttta ggtcacgaac ataaaccggt cggttcgttc ctgtttgccg
    45541 gtcctaccgg ggtcgggaaa acagaggtga cggtacagct ttcgaaagcg ttgggcattg
    45601 agcttctgcg ctttgatatg tccgagtata tggaacgcca taccgtcagc cgtctgattg
    45661 gtgcgcctcc gggatacgtt ggttttgatc agggcggttt gctgaccgat gcggtaatca
    45721 agcatccaca tgcggtgctg ctgctggacg aaatcgagaa agcgcatccg gacgtgttca
    45781 atattctgtt gcaggtgatg gacaacggta cgctgaccga taacaacgga cgcaaagcgg
    45841 acttccgtaa cgtggtgctg gtgatgacca ctaacgccgg ggtacgggaa actgagcgca
    45901 aatccattgg tcttatccac caggataaca gcaccgatgc gatggaagag atcaagaaga
    45961 tctttacacc ggaattccgt aaccgtctcg acaacattat ctggttcgat catctgtcaa
    46021 ctgacgtgat ccatcaggtg gtggataaat tcatcgtcga gttgcaggtt cagctggatc
    46081 agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa ctggctggcc gagaaaggtt
    46141 acgaccgggc aatgggcgca cgtccgatgg cgcgtgtcat ccaggacaac ctgaaaaaac
    46201 cgctcgccaa cgaactgctg tttggttcgc tggtggacgg cggtcaggtg acggttgcgc
    46261 tggataaaga gaaaaatgag ctgacttacg gattccagag tgcacaaaag cacaaggcgg
    46321 aagcagcgca ttaatctgat tgtcaggtag gttggtgaag tccgtaatct cgtcaggggt
    46381 tacggacttt ttatttatgg ggtggaacgg tgaaaaccct atttttggag gtgaaggtaa
    46441 gttgttgata attagtgctg ctggaaggta aggataaaaa agggtgctgc aggagaatgg
    46501 gatggttttg ctttattaac aacgggctaa acgtgtagta tttgagttca ctgccgtaca
    46561 ggcagcttag aaatcgacgg ggtgcggtaa aacctttgcg aacgcgttca ctgccgtaca
    46621 ggcagcttag aaattcacag gtaacatact ccacccgccc accatgttca ctgccgtaca
    46681 gacagataaa atgcgaaaaa aaagctcgca ctttcgtacg agctcttctt taaatatggc
    46741 ggtgaggggg ggattcgaac ccccgatacg ttgccgtata cacactttcc aggcgtgctc
    46801 cttcagccac tcggacacct caccaaattg ttttgctacc aaacctcatg ggtggcaacg
    46861 gggcgctact atagggagtt ggagtaaaac ggtcaagaag aattttaatg ataattattg
    46921 tttgctcata ctgtaaacaa cttgtgcagt atatctacat cgagacaagt tatggactta
    46981 tacttccaaa gtacttcata catatcacaa aataaaaagg ccggttaaac cgacctttta
    47041 ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca gcgactacgg aagacaatgc
    47101 ggcctttgct caggtcgtac ggggtcagtt caacagtcac tttgtcgccc gtcaggatgc
    47161 ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt aaccacgtga ccgttttcta
    47221 actctacgcg gaacatggta ttaggcaacg tttcaagaac ggtaccttgc atttcaatat
    47281 tgtcttcttt ggccatctaa tcctctgggg tatcactacc gtaatttgaa ccggcaagat
    47341 aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta aatcgcaaca aacaggttcg
    47401 gcacattact ccgaaaacac acggctaagc cgcaccaaaa gcgcaacgta taagggagcg
    47461 gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc ttatcggcag cggggtaatg
    47521 agcgtaacca actctgcgac cgcaattata acactctggg gagaaatgtg ccgaaaacat
    47581 tcattcttgt ggtgaaaaca agcaccgtgg tacccagaaa ttattcggca atcgtccgag
    47641 gcgcatttga ttgagataat taaggtaatc ccggcgggga atttcgcagg caccaagcga
    47701 tgctgtgtga tcgttaagga cctggcagtc gataagctta ccgccatgac cgataaattc
    47761 ctcacagaat accagaagcg ccgttttaga cgcattttcc atccggctga acatggactc
    47821 gccacaaaat agcgttccct gggccacgcc gtacataccg ccgacaagct catcttcacg
    47881 ccagacttca atggagtggg catgaccgag ttcgtgaagg cgatggtagg cttcgaccac
    47941 gccacgcgtg atccaggttc cttcttcgcg atcgctggca cagccttcaa tgacctgacc
    48001 aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta tgaaatcgct tcatactacg
    48061 gctgatatgc agtgattctg gccatagcac cgcgcgggga tcgggcgacc accagaggat
    48121 ggggtcgcct ggagaaaacc acggaaaaat accacgctgg taagccatta acaggcgcgc
    48181 agggctaaga tcgcccccaa gtgccagcag gccgttaggc tcacgtaatg cgccttccgg
    48241 ggaagggaag gctattgaat ggcgagaaag ctgaaccagg cgcatgaccg caaaactcca
    48301 cgcaagtcgg atcgttcaat aatagcttac aaaccctgct tgaactggta ataacgcccc
    48361 tgtctggcaa gcagttctgc gtgagtacct tgctcaataa tttgcccgtt gtccatcact
    48421 attatttgtt ggaaacgaga gagtccgcga agtcgatggg tgaccattaa caccgttttc
    48481 tcacgcatca tttttgcaag caattcaagg atctggcttt cggttgtggc atctaagcct
    48541 tcggtaggtt catccagcaa caccagtggc gcatcatgta acagcgcacg ggcgatagcc
    48601 agacggcgca gttcaccacc ggagagctgg cgtccgcctt cacctaacca actgttgaga
    48661 cctgcatcct cgagcagctt ctccagacca acgcgacgca agatctccga cagagcctca
    48721 tcactactgc caggcgaggc gagtaacaga ttatcacgca gcgtggcgct aaacagatgc
    48781 actcgctgag gaacaacgct gatggtctgt cgtagagccg cttcattcag gctggctatg
    48841 gggctatcgt taagcaaaat ctcgccctgt tgcgggtccc atgcgcgggt cagcagttgt
    48901 aacagtgttg atttgccgca tccggttcgc ccgagaatcg ctatatgttc cccggcgttt
    48961 acctgaagag aaatcccttt aagtgcctgt tgggattgct ccggataagt gaactgaaca
    49021 tcccgtaatg tcagcgaaac gcgatcggca acacgagttt gggtatcagg gaaggtgacc
    49081 tccggttttt gatccgttaa gtcagagata cgtacggcag aggcaatgac ttgccccaga
    49141 tgctgaaatg cacccgttac tggtgccagt gcttcaaacg cggctaacgc acaaaagaca
    49201 aacagggcaa ttaatgcgcc gggttgagca ttgccgccaa cgccgccaga cgccatccac
    49261 agcatcagga tcaccgctaa cgcgccaatg agcagcatta tcgcttgcga caatgcggtc
    49321 agttcagatt gacggcgttg cgcttccagc cattgaattt ctgtattctc tagttgcgtg
    49381 cgataacgat cgctggcacc aaaaatggtc agctcagctt gcccttgcag ccaggccgtc
    49441 agttgttggc gatactgtcc gcgaagatga gtcagatttt gcccggtgct ttttcccgca
    49501 cgataaaaca gcggtggcat caggaaaagc gtcagtaaca taacgctgcc cagcgtaaag
    49561 gcgagggtga aatcaaggaa acttaacccg attgtcacca ccataatcac cacaaaagcg
    49621 cccaccagcg gcgagataac gcgcaggtaa agatgatcga gcgtatcaac atccgccacc
    49681 acgcggttga gcaattcgcc ctgacgatag cgcgccagtc cggcagggga gaggggcaac
    49741 aatttgctga aggtgtaaat gcgcagatgc tgcaacacgc ggaaagtcgc gtcgtgactt
    49801 accagacgtt caaaataacg cccggcagta cgggtgattg cggcaccacg cacgcccgca
    49861 gcgggtagca tatagttgaa gctgtacagt ccggcaaccc ccgcaaccgc tgaggccgag
    49921 aggaaccagc cggaaagtgt caacagaccg atactggcga gctgcgtcac aattgccagc
    49981 acaataccaa gacttaacat ccatttatga cgtttataca gtgccagata gggtagcaaa
    50041 gcgcgcattt aaatctcctc ctgacgatgg gccagtaatg tggcgaatgg gccaccagcc
    50101 acacttaatt ccgcgtaacg tccttgctca ataatccggc catcctgcat aacccaaatg
    50161 acatcccagt cagcaagatc ttctaactgg tgggtgacca ttaacgttgt ctggcgcaga
    50221 gaggcggcat tcagcgcctc cattacgcgc tgttcactgt gagcatcaag gctggcagcg
    50281 ggttcatcca acagtaatag cgaacaggga tttagtaacg cacgggccac cgccacgcgc
    50341 tgcgcctgcc ccacggaaag gcgggcagcc tggtcgccaa caggtgtatc aacgccttgt
    50401 ggcaggagcg gtagaaactc gctgacccag gcgttatcca gcgctgcttg taattcttgt
    50461 tcgctggcat caggtcgcgc cagtagtacg ttatcccgca attttgctgc cggtaattgt
    50521 gggttttgcc caacccagga gagatgttta cgccatgatt ctggtgataa atcacgtaat
    50581 tctatcccgt tgattcgtaa tgatccctga tatgagagaa aaccagaaag cgcgttcagc
    50641 agtgagcttt tacctgaacc gctgcggcca accaacactg cacgttggcc tgctggcaaa
    50701 gtaaagttca gcggtccggc cagcgtttta ccttccggcg acgtgataaa cagatcctcg
    50761 gcctcaatgg tcagcggatc ggtcaatgct aattccgcct caccgcgctg cggatgggcg
    50821 agcggggttt ccataaacgt tttcagactg tcagctgcac caacagcctg ggctttagca
    50881 tgataaaacg taccgagatc gcgtaatggc tggaaaaact ctggcgcaag gatcagggcc
    50941 agaaaacctg cagccagcgt caccccagta tcgtagtgac caaaatccag ctcgccgaga
    51001 taggaaaaac caaagtagac cgccaccaga gcaattgaca gcgaggtaaa aaattcgaga
    51061 atgccggagg ataaaaacgc cagtcgtagc acttccattg tccgttggcg gaaatcttcc
    51121 gaagcagaac gaatactttc aatttcagct tcaccacgac caaaaatacg caatgtttcc
    51181 atgccgcgca ggcgatcgag gaaatgccca cttaagcgag caagagcgag aaagttacgc
    51241 cggttagcat cggcagcccc cattccaacc agcgccataa acaacggaat tagcggtgct
    51301 gtgcccagca gaatgagcgc cgcagcccag ttagagggga agatcgccac cacaatcagc
    51361 aacggcaccg acactgccag cgccatttgc ggcagatagc gtgcatagta atcatgcata
    51421 tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg caggtttacc ctgaatccac
    51481 gctggccctg cttgttgcag acggtcgaga acctgacggc ggatggcaaa gcggatatgc
    51541 tgcccggcgt gataacccac ccgttcgcgt aaccagacca cccatgcgcg caatacaaag
    51601 gtcagaacca gtaacgtaaa gggaagcagc agggcttcac ggggaatatt ctccataatc
    51661 atatgttgca gaattcgcgc catgaaccag gcctgggcaa tgatcaatat gccgcttaca
    51721 aagcccagca gacgagaaat attcagccaa cgttgggaga tgacgctttg ctgttttaac
    51781 cagcgggtta actctttttg acgagattta ttcattgcac gcttagcagg tgagttatca
    51841 gaattatttg cagagcaatg ttacaacggg gaaaaaataa aggcgaccca tagtcgcaag
    51901 gtgtcgcctt ctttactttt gttactgatt tgtaaaatta ttttgcgtca gctaaaccat
    51961 cgaggtagcg ttccgcatca agtgctgcca tgcagccagt accggccgaa gtaatggcct
    52021 ggcgataaat gtgatccatc acgtcgcctg cggcaaagac gccaggaatg ctggtctggg
    52081 tggcattacc atgaataccc gactgtactt tgatgtagcc gttttccagt tccagctgcc
    52141 cttcgaaaat cgcagtattc gggctgtgac cgatagcaac aaacagaccg gcaacgtcga
    52201 gtgactcgat gttatcgctg ttttgcgtat cgcgcagacg aacgccagtg acacccattt
    52261 gatcgccggt cacttcttcc agcgtacggt tggtgtgcag aatgatgttg ccgttctcca
    52321 ctttatccat cagacgctta ataaggattt tttccgcgcg gaaaccgtca cggcggtgaa
    52381 tcagatgcac ttccgaagcg atgttagaca gatacagcgc ctcttcaacc gcggtattgc
    52441 cgccgccgat gaccgcaact ttctggttgc gatagaagaa accgtcgcag gttgcacaag
    52501 cagaaacccc acggccttta aaggcttctt cagagggcag gccgagatag cgtgcagaag
    52561 ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc gttatcgcca ttcagacgga
    52621 acggacggtt ttgcagatcc accttgttga tatgatcaaa aatgatctca gtttcaaact
    52681 tggtggcatg ttcgtgcatg cgcgccatta ataacggacc ggtcagatcg tttggatcac
    52741 caggccagtt ttccacttcc gtggtggtgg tcagttggcc gcctttttcc atgccggtaa
    52801 tcagcacagg ttgcaggttg gcgcgcgccg cgtagacagc agcggtgtat cccgccgggc
    52861 ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc catgagatcc ccatagttgt
    52921 tggcagacaa tgggcaggat tgtagggaat ttacagacgt aaaaaaagag tatgacgatt
    52981 ttgttaacaa tttgtgcaat cggcagcatc gataagcagg tcaaattctc ccgtcattat
    53041 cacctctgct acttaaattt cccgctttat aagccgatta aatgatgaat aaacgcccct
    53101 gttaatgaat atctggcatg ttgtactaaa aattgatgtt ttgctttgac aatcccctgg
    53161 tgttttgcga aaacattcga ggaagaaaaa aaacagtatt cttatatgcg cataaccatg
    53221 tatgtaaata ccatgtttac cgtgctagtg aaatctacgt atggcgtgga cagacgccat
    53281 tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca ccgtagaccc aggcattgcg
    53341 cgccgtgaat cttcatgatt tcggtctatc gtgacgggta gcgactctga acagtgatgt
    53401 ttcagggtca gacaggagta gggaaggaat acagagagac aataataatg gtagatagca
    53461 agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa cattcttaat gagttgcaaa
    53521 aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt gggactttcc ccaacgccgt
    53581 gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat tcagggctat acggcgctgc
    53641 ttaaccccca ttatctggat gcatcacttc tggtattcgt tgagattact ctgaatcgtg
    53701 gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca aaaacttgaa gaaattcagg
    53761 agtgtcattt agtatctggt gatttcgact acctgttgaa aacacgcgtg ccggatatgt
    53821 cagcttaccg taagttgctg ggggaaaccc tgctgcgtct gcctggcgtc aatgacacac
    53881 ggacatacgt tgttatggaa gaagtcaagc agagtaatcg tctggttatt aagacgcgct
    53941 aacacggaac aggtgcaaaa tcggcgtatt ttgattacac tcctgttaat ccatacagca
    54001 acagtactgg ggtaacctgg tactgttgtc cgttttagca tcgggcagga aaagcctgta
    54061 acctggagag cctttcttga gccaggaata cactgaagac aaagaagtca cattgacaaa
    54121 gttaagtagc ggccgccgcc ttctggaggc gttgctgatc cttattgtcc tgtttgccgt
    54181 ctggttgatg gctgccttac taagctttaa cccttcggac cccagctggt cgcaaacggc
    54241 ctggcatgaa cctatccata atttaggtgg gatgcccggt gcgtggttgg cagatacgct
    54301 gttctttatt tttggcgtga tggcttacac cattcccgtc attattgtcg gcggttgttg
    54361 gtttgcctgg cgtcatcagt ccagcgacga atacattgat tattttgccg tttcgctacg
    54421 catcattggc gttttggcgc tcatccttac ctcctgtggt ctggcggcaa tcaacgctga
    54481 cgatatctgg tattttgcct ccggtggcgt cattggcagc ttactaagca ctacgctaca
    54541 accactgcta cacagtagcg ggggaactat tgcgctgctc tgcgtttggg cagcgggcct
    54601 gacgttgttc accggttggt catgggtgac cattgctgaa aaactcggcg gctggatttt
    54661 aaacattctc accttcgcca gtaatcgtac ccgtcgcgat gatacctggg tcgatgaaga
    54721 tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac ggcaaacagc atgaatcacg
    54781 ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa cggttggcgg aaaaattcat
    54841 taatccgatg gggcggcaaa cagacgctgc gttgttctcc ggtaagcgga tggatgatga
    54901 cgaagagatt acctacactg cacgcggtgt ggctgccgac ccggacgacg tcctattttt
    54961 gggcaatcgt gcaacgcagc cagaatatga cgaatacgat ccattattaa acggtgcgcc
    55021 aattaccgaa cctgtcgctg tagcagctgc tgctaccacg gcgacacaaa gctgggctgc
    55081 gccggttgaa cctgtgactc agacgccgcc tgttgcctct gttgatgttc caccttcgca
    55141 acctacagta gcctggcagc ctgtaccggg tccacaaacg ggagagccgg ttattgctcc
    55201 tgcaccggaa ggttacccac agcagtcaca atatgcgcag cctgcagtgc aatataatga
    55261 gccgctgcaa caaccagtac agccgcagca gccgtattat gcacctgcag ctgaacaacc
    55321 tgcgcaacaa ccttactacg ctcctgcggc ggaacaacct gttcagcaac cgtattacgc
    55381 aactgcgccg gaacagcctg cgcaacagcc gtattatgcc cctgcgccag aacaaccggt
    55441 ggcaggtaac gcctggcaag ccgaagagca gcaatccatt tttgctccac agtctacata
    55501 ccagactgag caaacttatc agcagccagc cgctcaggag ccgttgtacc aacagccgca
    55561 acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt gtagaagaga caaaacccgc
    55621 gcgtccgccg ctttactatt ttgaagaagt ggaagagaag cgagcccgtg aacgtgaaca
    55681 acttgcggcc tggtatcaac cgattccaga accggttaaa gaaccagaac cgatcaaatc
    55741 ttcgctgaaa gcaccttctg ttgcagcagt acctccagta gaagccgctg ccgctgtttc
    55801 tccgctggca tctggcgtga aaaaagcgac actggcgacg ggggctgccg caaccgttgc
    55861 cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt ccgcaggtca aagaggggat
    55921 tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca actcgtcgtg aattggcgtc
    55981 ttacggtatt aagctgccct cacagcgtgc ggcggaagaa aaagcccgtg aagcccggcg
    56041 caatcagtat gattctggcg atcagtataa cgatgatgaa atcgatgcga tgcagcagga
    56101 tgaactggca cgtcagttcg cccagacaca gcagcaacgc tatggcgaac agtatcaaca
    56161 tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca gaggctgaac tggctcgtca
    56221 gtttacgcaa actcaacaac aacgttattc cggcgaacaa ccggctgggg cgaatccgtt
    56281 ctcgctggat gattttgaat tttcgccaat gaaagcgttg ctggatgatg gtccacacga
    56341 accgctgttt acgccaattg ttgaacctgt acagcagccg caacaaccgg ttgcaccaca
    56401 gcagcagtat cagcagccac aacagccggt tgcgccacag ccgcagtatc agcagccaca
    56461 acagcaggtt gcgccgcagc cacaatatca gcagccgcaa caaccggttg cgccacagca
    56521 gcaatatcag cagccgcaac aaccagttgc gccgcagccg cagtatcaac agccgcaaca
    56581 accggttgca ccgcagccgc agtatcagca gccacaacag ccagttgcgc cacagccgca
    56641 ggataccctg cttcatccgc tgttgatgcg taatggcgac agccgtccgt tgcacaaacc
    56701 gacgacgccg ctgccttctc tggatttgct gacaccgccg ccgagcgaag tggagccggt
    56761 agataccttt gcgcttgaac aaatggcgcg tctggtggaa gcgcgtctgg ctgatttccg
    56821 tattaaagcc gatgtcgtta attactctcc ggggccagtt atcactcgct ttgaacttaa
    56881 cctggctcct ggcgtaaaag cggcgcgcat ttctaacttg tcacgggacc ttgcccgttc
    56941 actttcgacg gtggcggtgc gtgtcgttga agttattcct ggcaaaccct atgtaggtct
    57001 ggagttacca aataaaaaac gacaaaccgt ttatctgcgc gaagttttgg ataacgccaa
    57061 attccgcgat aatccgtcgc cattaaccgt ggtgctgggt aaagatatcg ccggtgagcc
    57121 ggtggttgcc gatctggcga aaatgccgca cttgttggtt gcggggacta ccggttccgg
    57181 taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt tataaagcac agccagaaga
    57241 tgtgcgtttc atcatgatcg acccgaaaat gctggagctt tcggtttatg aaggcattcc
    57301 gcacctgtta acggaagtcg ttactgatat gaaagatgcc gccaacgcgc tgcgctggtg
    57361 tgttaacgag atggagcgtc ggtataaact gatgtctgcg ctgggtgtgc gtaatctggc
    57421 gggttataac gaaaaaattg ctgaagccga tcgcatgatg cgtccgattc cagacccgta
    57481 ctggaagccg ggtgacagta tggatgccca gcatccggtg ctgaaaaaag aaccatacat
    57541 tgtcgtgttg gttgacgaat ttgccgacct gatgatgacg gtaggtaaaa aagtggaaga
    57601 gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt atccacctcg tactggcaac
    57661 tcagcgtcca tcggttgatg ttattactgg tctgattaaa gcgaatattc cgacccgtat
    57721 cgcctttacc gtatccagta agattgactc acgtaccatt cttgatcagg ctggcgcgga
    57781 atcactgctg ggtatggggg atatgctcta ctctgggccg aactccacgt tgccggtacg
    57841 tgtccatggt gcttttgttc gcgatcagga agttcatgcc gtggtgcagg actggaaagc
    57901 gcgtggtcgc ccacagtatg ttgatggcat cacctccgac agcgaaagcg aaggtggtgc
    57961 gggtggtttc gatggcgctg aagaactgga tccgttgttc gatcaggcgg tgcagtttgt
    58021 cactgaaaaa cgcaaagcgt caatttctgg cgtacagcgt cagttccgca ttggttataa
    58081 ccgtgcagcg cgtattatcg aacagatgga agcgcagggg attgtcagcg aacaggggca
    58141 caacggtaat cgtgaagtgc tggccccacc gccgtttgac taattaatgc attgccggat
    58201 aaggcgcggt agcgtcgcat ccggcactct atcaactgaa aattcagtat tttcttcttt
    58261 cctcaagctg attattagcc tggaatagag agtagaggga actcccgatc gggagtgacg
    58321 taatttgagg aataatgatg aaaaaaattg ctatcacctg tgcattactc tcaagcttag
    58381 tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa aagccgcctg gataaagtca
    58441 gcagcttcca cgccagcttc acacaaaaag tgactgacgg tagcggcgcg gcggtgcagg
    58501 aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt caactggcat atgacacaac
    58561 ctgatgaaag cattctggtt tctgacggta aaacactgtg gttctataac ccgttcgttg
    58621 agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa tacgccgttt atgctgattg
    58681 cccgcaacca gtccagcgac tggcagcagt acaatatcaa acagaatggc gatgactttg
    58741 tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt caccattaac gtgggacgtg
    58801 atggcacaat ccatcagttt agcgcggtgg agcaggacga tcagcgcagc agttatcagc
    58861 tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt taccttcacc ccgccgcaag
    58921 gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag tgagcaatct gtcgctcgat
    58981 ttttcggata atacttttca acctctggcc gcgcgtatgc ggccagaaaa tttagcacag
    59041 tatatcggtc agcaacattt gctggctgcg gggaagccgt tgccgcgcgc tatcgaagcc
    59101 gggcatttgc attctatgat cctctggggg ccaccgggta ccggcaaaac aaccctcgct
    59161 gaagtgattg cccgctatgc gaacgctgat gtggaacgta tttctgccgt cacctctggc
    59221 gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa accgcaatgc aggtcgccgc
    59281 actattcttt ttgttgacga agttcaccgt ttcaacaaaa gccagcagga tgcatttctg
    59341 ccacatattg aagacggcac catcactttt attggcgcaa ccactgaaaa cccgtcgttt
    59401 gagcttaatt cggcactgct ttcccgtgcc cgtgtttatc tgttgaaatc cctgagtaca
    59461 gaggatattg agcaagtact aactcaggcg atggaagaca aaacccgtgg ctatggtggt
    59521 caggatattg ttctgccaga tgatacacga cgtgccattg cagaactggt gaatggcgac
    59581 gcgcgccggg cgttaaatac gctggaaatg atggcggata tggccgaagt ctatgatagc
    59641 ggtaagcggg tcctgaagcc tgaattactg accgaaatcg ccggtgaacg tagcgcccgc
    59701 tttgataaca aaggcgatcg cttttacgat ctaatttccg cactgcataa gtcggtacgt
    59761 ggtagcgcac ccgatgcggc gctgtactgg tatgcgcgaa ttattaccgc tggtggcgat
    59821 ccgttatatg tcgcgcgtcg ctgtctggcg attgcgtctg aagacgtcgg taatgccgat
    59881 ccacgggcga tgcaggtggc aattgcggcc tgggattgct ttactcgcgt tggcccggcg
    59941 gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg cctgcgcgcc aaaaagcaac
    60001 gctgtctaca ctgcgtttaa agccgcgctg gccgatgctc gcgaacgccc ggattatgac
    60061 gtgccggttc atttgcgtaa tgcgccgacg aaattaatga aggaaatggg ctacgggcag
    60121 gaatatcgtt acgctcatga tgaagcaaac gcttatgctg ccggtgaggt ttacttcccg
    60181 ccggaaatag cacaaacacg ctattatttc ccgacaaaca ggggccttga aggcaagatt
    60241 ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata gccccataaa acgctaccgt
    60301 taatgttatc gttgcggtaa tgttgttact gtatccctgt ggtcgcaggc tgtggccaca
    60361 tctcccattt aattcgataa gcacaggata agcatgctcg atcccaatct gctgcgtaat
    60421 gagccagacg cagtcgctga aaaactggca cgccggggct ttaagctgga tgtagataag
    60481 ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca aaacggaaaa cctgcaagcg
    60541 gagcgtaact cccgatcgaa atccattggc caggcgaaag cgcgcgggga agatatcgag
    60601 cctttacgtc tggaagtgaa caaactgggc gaagagctgg atgcagcaaa agccgagcta
    60661 gatgctttac aggctgaaat tcgcgatatc gcgctgacca tccctaacct gcctgcagat
    60721 gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag tcagccgctg gggtaccccg
    60781 cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg gtgaaatgca ctctggcctc
    60841 gactttgcag ctgcagttaa gctgactggt tcccgctttg tggtaatgaa agggcagatt
    60901 gctcgcatgc accgcgcact gtcgcagttt atgctggatc tgcataccga acagcatggc
    60961 tacagtgaga actatgttcc gtacctggtt aaccaggaca cgctgtacgg tacgggtcag
    61021 ctgccgaaat ttgctggcga tctgttccat actcgtccgc tggaagaaga agcagacacc
    61081 agtaactatg cgctgatccc aacggcagaa gttccgctga ctaacctggt gcgcggtgaa
    61141 atcatcgatg aagatgatct gccaattaag atgaccgccc acaccccatg cttccgttct
    61201 gaagccggtt catatggtcg tgacacccgt ggtctgatcc gtatgcacca gttcgacaaa
    61261 gttgaaatgg tgcagatcgt gcgcccagaa gactcaatgg cggcgctgga agagatgact
    61321 ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt accgtaaaat catcctttgc
    61381 actggcgaca tgggctttgg cgcttgcaaa acttacgacc tggaagtatg gatcccggca
    61441 cagaacacct accgtgagat ctcttcctgc tccaacgttt gggatttcca ggcacgtcgt
    61501 atgcaggcac gttgccgcag caagtcggac aagaaaaccc gtctggttca taccctgaac
    61561 ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa tggaaaacta tcagcaggct
    61621 gatggtcgta ttgaagtacc agaagttctg cgtccgtata tgaacggact ggaatatatt
    61681 ggctaatacc caatttttct gaatctaaaa agcgccttta gggcgctttt tttgtctccc
    61741 tttgataccg aacaataatt actcctcact tacacgtaat actactttcg agtgaaaatc
    61801 tacctatctc tttgattttc aaattattcg atgtatacaa gcctatatag cgagctgcta
    61861 tagaaataat tacacaatac ggtttgttac tggaatcaat cgtgatcaag cttgagtgag
    61921 ccattatgaa aacgaaaatc cctgatgcgg tattggctgc tgaggagagt cgccgtggtt
    61981 tggtaaaaac gacagcgatc ggcggcctgg caatggccag cagcgcatta acattacctt
    62041 ttagtcggat tgcgcacgct gtcgatagcg ccattccaac aaaatcagac gaaaaggtta
    62101 tctggagcgc ctgtacagtt aactgtggta gtcgctgccc gctacgtatg cacgtcgtgg
    62161 acggtgaaat caaatatgtc gaaacggaca ataccggcga tgacaattac gacggcctgc
    62221 actaggttcg cgcctgcctg cgtgggcgtt ccatgcgtcg ccgtgtctac aatccggacc
    62281 gcctgaaata tccgatgaaa cgagtcgggg cgcgcggtga aggcaaattc gagcgcatta
    62341 gctgggaaga agcctacgac atcatcgcga ccaatatgca gcgcctgatc aaagagtacg
    62401 gcaacgagtc tatctatctg aactatggca ccggtacgct gggcggcacc atgacccgct
    62461 cctggccgcc gggaaatacc ctggtcgcgc gcctgatgaa ctgctgcggc ggttatctta
    62521 accattacgg tgactactct tccgcgcaaa ttgcggaagg tttgaaccat acctacggcg
    62581 gctgggcaga tggtaacagc ccgtcggata tcgaaaacag taaattggta gtgctgtttg
    62641 gtaataaccc aggcgaaacg cgaatgagtg gcggtggggt gacttactat cttgaacagg
    62701 cacgccagaa atctaatgct cgcataatca tcatcgatcc gcgctatacc gacaccggtg
    62761 ccgggcgcga agatgagtgg atccctattc gtccaggaac agatgccgca ctggttaacg
    62821 gtctggcgta cgtcatgatc actgaaaacc tggtggatca ggcattcctc gataaatatt
    62881 gcgttggcta tgacgagaaa accctgcccg ccagtgcgcc gaaaaatggt cattataaag
    62941 cttatattct gggtgaaggg ccagatggcg tggctaaaac accggaatag gcctcgcaaa
    63001 tcaccggtgt tccggcagac aaaatcatca aactggctcg tgaaatcggc agtaccaaac
    63061 cggcgtttat cagccaggga tggggcccgc agcgtcacgc taacggtgaa atcgcaaccc
    63121 gtgctatctc gatgctggcg attctgaccg gtaacgttgg tattaatgga gggaacagcg
    63181 gcgcgcgtga aggttcatac agcttaccgt ttgtccgtat gccgaccttg gaaaacccga
    63241 tccagaccag catttcgatg tttatgtgga ccgatgccat tgaacgtggc ccggaaatga
    63301 cggcgctgcg tgatggtgtg cgcgggaaag ataagctgga tgtgccgatc aaaatgatct
    63361 ggaactatgc cggtaactgc ctgattaacc agcattctga aatcaaccgt acccatgaaa
    63421 tcctccagga tgataagaag tgcgagctga ttgtggttat cgactgccac atgacctcat
    63481 cggcgaaata tgctgacatc ctgctgcctg actgcaccgc ttccgaacag atggactttg
    63541 cactggatgc atcctgcggg aatatgtctt acgtgatttt caacgatcag gtgattaaac
    63601 cgcgctttga atgtaagacc atctatgaaa tgaccagcga actggcaaaa cgtcttggcg
    63661 ttgagcaaca gtttactgaa ggccgtaccc aggaagagtg gatgcggcat ctgtatgccc
    63721 agtcgcggga agcgattcct gaactgccaa cgtttgaaga gttccgcaag caggggatct
    63781 ttaaaaagcg cgacccacaa gggcatcacg ttgcttataa agccttccgt gaagatccgc
    63841 aggcaaaccc actgactacg ccatcgggca aaattgagat ttattcgcag gcgctggctg
    63901 acattgccgc tacctgggaa ttgccagaag gcgatgtgat cgatccactg ccgatctaca
    63961 cgccgggctt tgaaagttat caggatccgc tgaacaaaca gtatccgctg cagcttacag
    64021 gtttccacta taaatctcgc gttcactcaa cttacggcaa cgttgatgtg ctgaaagcgg
    64081 cttgccgtca ggaaatgtgg atcaacccgc ttgatgccca aaaacgcggt atccacaacg
    64141 gcgataaagt caggatcttt aacgatcgtg gtgaggttca tattgaggcg aaagtgacgc
    64201 cacgaatgat gccgggcgtg gtcgcactgg gtgaaggcgc ctggtatgac ccggatgcaa
    64261 aacgtgtcga taagggcggt tgtatcaacg tactgaccac tcaacgtccg tctcctctcg
    64321 ctaaggggaa tccgtcacat acaaaccttg ttcaggttga aaaggtgtaa ggagtaaccg
    64381 atgacaaccc agtatggatt ttttattgat tccagccgtt gcaccggttg caaaacctgc
    64441 gagctggctt gtaaagacta caaagatttg acgccagaag tcagtttccg ccgcatttat
    64501 gaatatgctg gcggcgactg gcaggaagat aacggtgtct ggcaccagaa cgtgtttgcc
    64561 tactatctgt cgatttcgtg caaccactgc gaagatccgg cttgtactaa agtctgcccg
    64621 agcggtgcga tgcataaacg cgaagatggt tttgttgtgg tcgatgaaga tgtatgcatt
    64681 ggctgccatt actgtcatat ggcttgcccg tatggcgcac cgcaatataa cgaaacgaaa
    64741 ggccatatga ccaaatgcga tggttgttat gaccgtgttg cggagggtaa aaagccgatc
    64801 tgtgttgaat cttgcccgct gcgggcgctg gatttcggcc ctatcgacga gctgcgtaaa
    64861 aaacatggcg atctggcggc cgttgcgccg ttgccgcgag ctcactttac caaaccgaat
    64921 attgtgatca aacccaatgc caatagccgc ccgacagggg ataccactgg ctatctggca
    64981 aacccgaagg aggtgtgaga tgggaagtgg atggcatgaa tggccgctga tgatcttcac
    65041 ggtcttcggg caatgtgtgg cgggtggttt tatcgttctg gctttggcgc tgctcaaagg
    65101 cgacctgcga gcagaagccc agcagcgtgt tatcgcctgc atgtttggct tatgggtact
    65161 gatgggcatt ggctttatcg cctctatgct ccatcttggt tcaccgatgc gcgcctttaa
    65221 ctcgctcaac cgggtagggg cttcagcact cagtaacgaa atcgccagcg gttcgatctt
    65281 ttttgctgta ggcggcatcg gctggttgct ggcaatgctg aaaaagctgt caccggcatt
    65341 gcgtacgcta tggctggtgg tgacgatggt tcttggcgtc gtctttgtct ggatgatggt
    65401 gcgtgtgtat aacagcattg ataccgttcc gacctggtac agcatctgga cgccgatggg
    65461 cttcttcctg acgatgttca tgggcggccc gttactgggt tacctgttgc tgagtctggc
    65521 tggcgtcgat ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt
    65581 gagtggcgtg atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca
    65641 gcaggccgca gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt
    65701 ggccgttgcc ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc
    65761 gctactatcc gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt
    65821 ctatggtttg catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt
    65881 ttcggccccg gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt
    65941 acgataatca ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca
    66001 gtcacggtgc agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga
    66061 catgcgatcc cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac
    66121 gacaaagaca tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc
    66181 ggttaccaca ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa
    66241 atcttcgtta tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa
    66301 ttgtgctttc agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag
    66361 aatagttggt aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa
    66421 cttatcgggt tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa
    66481 aacggcagca tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg
    66541 agagtcagtg gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg
    66601 ggaaatatga gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg
    66661 tgttagatca ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga
    66721 atgagtaata taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac
    66781 aatgtttttg gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca
    66841 tgtccacgta tacccggcct gtcatgcttt tactgtctgg cctgcttttg ttgactctgg
    66901 cgatcgcggt gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat
    66961 ggcaggtagg cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga
    67021 cagggtatgt cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt
    67081 ttgctgctgg ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc
    67141 gttttgtcgc gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt
    67201 gcagtgggac gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg
    67261 tgggaacgtt tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat
    67321 tgccgtgggt tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc
    67381 tgaatcagca ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc
    67441 aggcgcggct tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg
    67501 gcctgatgcc tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga
    67561 tggcggtact ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata
    67621 agtttggtcg actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg
    67681 cgatgcttag ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta
    67741 cgctatatcc ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg
    67801 cgatgaacca agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta
    67861 ccgctatgct aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat
    67921 cgtttatcta tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc
    67981 acgtgtaaat gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt
    68041 cttctatctg acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg
    68101 ttataatttt gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt
    68161 tacagatgcc cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcaggaagt
    68221 aa
//
LOCUS       DAJTYF010000007        66494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTYF010000007 DAJTYF010000000
VERSION     DAJTYF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.007"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO590_000551"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO590_000551"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6636038.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO590_000552"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO590_000552"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6636039.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO590_000553"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO590_000553"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6636040.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO590_000554"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO590_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6636041.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO590_000555"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO590_000555"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6636042.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDASHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO590_000556"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO590_000556"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6636043.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO590_000557"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO590_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6636044.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO590_000558"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO590_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6636045.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMDICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLN"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO590_000559"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO590_000559"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6636046.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO590_000560"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO590_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6636047.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO590_000561"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO590_000561"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6636048.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO590_000562"
     CDS             complement(15665..16702)
                     /locus_tag="OO590_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6636049.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO590_000563"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO590_000563"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6636050.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19304)
                     /gene="ggt"
                     /locus_tag="OO590_000564"
                     /pseudo
     CDS             complement(17582..19304)
                     /gene="ggt"
                     /locus_tag="OO590_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            19424..19864
                     /locus_tag="OO590_000565"
     CDS             19424..19864
                     /locus_tag="OO590_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6636051.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="OO590_000566"
     CDS             complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="OO590_000566"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6636052.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20591..21581)
                     /locus_tag="OO590_000567"
                     /pseudo
     CDS             complement(20591..21581)
                     /locus_tag="OO590_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="OO590_000568"
     CDS             complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="OO590_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6636053.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="OO590_000569"
     CDS             complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="OO590_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6636054.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="OO590_000570"
     CDS             complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="OO590_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR6636055.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25123..25836)
                     /gene="livF"
                     /locus_tag="OO590_000571"
     CDS             complement(25123..25836)
                     /gene="livF"
                     /locus_tag="OO590_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6636056.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSSYLGG"
     gene            complement(25838..26605)
                     /gene="livG"
                     /locus_tag="OO590_000572"
     CDS             complement(25838..26605)
                     /gene="livG"
                     /locus_tag="OO590_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6636057.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26602..27879)
                     /gene="livM"
                     /locus_tag="OO590_000573"
     CDS             complement(26602..27879)
                     /gene="livM"
                     /locus_tag="OO590_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6636058.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27876..28802)
                     /gene="livH"
                     /locus_tag="OO590_000574"
     CDS             complement(27876..28802)
                     /gene="livH"
                     /locus_tag="OO590_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6636059.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28850..29959)
                     /gene="livK"
                     /locus_tag="OO590_000575"
     CDS             complement(28850..29959)
                     /gene="livK"
                     /locus_tag="OO590_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6636060.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30383..30766
                     /gene="panM"
                     /locus_tag="OO590_000576"
     CDS             30383..30766
                     /gene="panM"
                     /locus_tag="OO590_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6636061.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30965..32068)
                     /gene="livJ"
                     /locus_tag="OO590_000577"
     CDS             complement(30965..32068)
                     /gene="livJ"
                     /locus_tag="OO590_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6636062.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="OO590_000578"
     CDS             complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="OO590_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6636063.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="OO590_000579"
     CDS             complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="OO590_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6636064.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="OO590_000580"
     CDS             complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="OO590_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6636065.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="OO590_000581"
     CDS             complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="OO590_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6636066.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36806..37402
                     /gene="rsmD"
                     /locus_tag="OO590_000582"
     CDS             36806..37402
                     /gene="rsmD"
                     /locus_tag="OO590_000582"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6636067.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37392..37661
                     /locus_tag="OO590_000583"
     CDS             37392..37661
                     /locus_tag="OO590_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6636068.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37664..38023)
                     /locus_tag="OO590_000584"
     CDS             complement(37664..38023)
                     /locus_tag="OO590_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6636069.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38164..38790
                     /locus_tag="OO590_000585"
     CDS             38164..38790
                     /locus_tag="OO590_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6636070.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38864..41062
                     /gene="zntA"
                     /locus_tag="OO590_000586"
     CDS             38864..41062
                     /gene="zntA"
                     /locus_tag="OO590_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6636071.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="OO590_000587"
     CDS             complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="OO590_000587"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6636072.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41630..42295
                     /locus_tag="OO590_000588"
     CDS             41630..42295
                     /locus_tag="OO590_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6636073.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42368..42925
                     /gene="dcrB"
                     /locus_tag="OO590_000589"
     CDS             42368..42925
                     /gene="dcrB"
                     /locus_tag="OO590_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6636074.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42929..44146)
                     /locus_tag="OO590_000590"
                     /pseudo
     CDS             complement(42929..44146)
                     /locus_tag="OO590_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44278..45327
                     /locus_tag="OO590_000591"
     CDS             44278..45327
                     /locus_tag="OO590_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6636075.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45382..45969
                     /gene="acpT"
                     /locus_tag="OO590_000592"
     CDS             45382..45969
                     /gene="acpT"
                     /locus_tag="OO590_000592"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6636076.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46080..47654
                     /gene="nikA"
                     /locus_tag="OO590_000593"
     CDS             46080..47654
                     /gene="nikA"
                     /locus_tag="OO590_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6636077.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47654..48598
                     /gene="nikB"
                     /locus_tag="OO590_000594"
     CDS             47654..48598
                     /gene="nikB"
                     /locus_tag="OO590_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6636078.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48595..49428
                     /gene="nikC"
                     /locus_tag="OO590_000595"
     CDS             48595..49428
                     /gene="nikC"
                     /locus_tag="OO590_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6636079.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLILGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49428..50192
                     /gene="nikD"
                     /locus_tag="OO590_000596"
     CDS             49428..50192
                     /gene="nikD"
                     /locus_tag="OO590_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6636080.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50189..50995
                     /gene="nikE"
                     /locus_tag="OO590_000597"
     CDS             50189..50995
                     /gene="nikE"
                     /locus_tag="OO590_000597"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6636081.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51001..51402
                     /gene="nikR"
                     /locus_tag="OO590_000598"
     CDS             51001..51402
                     /gene="nikR"
                     /locus_tag="OO590_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6636082.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51522..51881)
                     /locus_tag="OO590_000599"
     CDS             complement(51522..51881)
                     /locus_tag="OO590_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6636083.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51878..52153)
                     /locus_tag="OO590_000600"
     CDS             complement(51878..52153)
                     /locus_tag="OO590_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR6636084.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52212..53336)
                     /locus_tag="OO590_000601"
     CDS             complement(52212..53336)
                     /locus_tag="OO590_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6636085.1"
                     /translation="MRQLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="OO590_000602"
     CDS             complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="OO590_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6636086.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56068..57135)
                     /locus_tag="OO590_000603"
     CDS             complement(56068..57135)
                     /locus_tag="OO590_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6636087.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57501..59123)
                     /locus_tag="OO590_000604"
     CDS             complement(57501..59123)
                     /locus_tag="OO590_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6636088.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINKNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59385..60974)
                     /locus_tag="OO590_000605"
     CDS             complement(59385..60974)
                     /locus_tag="OO590_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6636089.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEESQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61358..62389
                     /locus_tag="OO590_000606"
                     /pseudo
     CDS             61358..62389
                     /locus_tag="OO590_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62923..63687)
                     /locus_tag="OO590_000607"
     CDS             complement(62923..63687)
                     /locus_tag="OO590_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6636090.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSANLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNGETYAICSEVLLYD"
     gene            63934..64695
                     /locus_tag="OO590_000608"
     CDS             63934..64695
                     /locus_tag="OO590_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6636091.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSLQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64802..65302)
                     /locus_tag="OO590_000609"
     CDS             complement(64802..65302)
                     /locus_tag="OO590_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636092.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65453..>66494)
                     /locus_tag="OO590_000610"
     CDS             complement(65453..>66494)
                     /locus_tag="OO590_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6636093.1"
                     /translation="STISLSQPLGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRG
                     ITFSINWAKNDLLTSHKWKVDQKLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQV
                     SLSGQNSGGVWWDVATNITNAHQSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLG
                     ANLSGGILITRDGLTFGQNVDGTLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPY
                     RRDDVILDEKTIGKNYDLPQTSQLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVP
                     FGAVISYSKDTENMAGIVGEDGIAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKS
                     ASGLYQIAATCL"
BASE COUNT        16156 a        17847 c        16322 g        16169 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgtaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaagtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgccgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgagg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta attcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagtag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatgtcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgaga ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cgcccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgccgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc taaaacaact tcctgagagc
    19261 agagcagcaa tggccacccg gcgtaaaaac gtcggtttta tcatcgttat tctccagaga
    19321 ttaaggggca accccagcta agcctggtat ataactctga attaatcatc gttttgtcgg
    19381 gaagcgagta aacttaaagg atatcttcag aggaggatac gcgatgaaac gacttctgat
    19441 tcttacggca ctcctgccgt ttgtcggctt tgcacagccc attaatactc tgaacaaccc
    19501 taaccagccg gggtatcaga tacccagcca gcagcggatg caaacccaga tgcagactca
    19561 gcaaatccag caaaaaggga tgctgaatca gcaactgaaa acgcaaactc agttgcaaca
    19621 gcagcattta gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt cacagccggg
    19681 ggagcgaaat cccgcccggc agcaaatgct gcccaacacc aacggcggga tgttaaacag
    19741 caaccgtaat ccggatagtt cgttgaatca gcagcatatg ctgccggaga ggagaaacgg
    19801 cgacatgctg aatcagccca gcacgccgca gcctgacatt ccgttgaaaa ctattgggcc
    19861 gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga tgcaatccac gccccagcgc
    19921 agcaactctg ccgcgcgctg gggtttattg acggtataaa ccagaatccg taatccggcg
    19981 tctttcaact gcatcactcg cgctttatcg agtaacttat gattgagatg aatagagacg
    20041 cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag caataaacca
    20101 cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag catcaatctc aaacgatgac
    20161 agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac cattttgccc
    20221 gttaatggtc cagtgccagt ggtgggtttg atttcgatat tcgccatcat cccgtgttcg
    20281 cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct cacctttaaa cgctttgctg
    20341 taccaactgc ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc cgcgacgccc
    20401 cagccgttgc tggtgcgttc gagattgtcg tcatggagca ggaagatctc gccatctttc
    20461 gataacttcg cgtcaaattc gatcatctta tgaccgtatt ttgccccgac gtcgattgcc
    20521 gccagggtgt tttccggggc cagcttaccg ccgccacgat gagcgacgat gcggggataa
    20581 ggccagttac tcatactcgt tgtcctgttt caccatcaaa aagatgcagc tgattttccg
    20641 ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc agtcgcacca
    20701 ccagcttctg cttgcgagct tagcgcaata tgttccgggc gaatgccgag ggtcatttta
    20761 cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc gtccagttcg
    20821 aaatgcgtgc cttcattatt catgcggcct gtcagcaggt tcatcgccgg gctgccgata
    20881 aaactcgcca caaacaggct ggcgggcttt tcgtagactt ccaccggcgt gccaatctgt
    20941 tcggcaacgc cgccgttcat caccattact cgctgggcga gcgtcatcgc ctcaacctga
    21001 tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcagctgttg cagttcaaga
    21061 cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg gctcatcaaa caagaacacc
    21121 gccggatcac gcacaatcgc gcgtcccatc gccacacgct ggcgctgacc gccggaaagc
    21181 tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa tgcgcgccgc ttctttaacg
    21241 cgctcggcta tttgctgctt gcccatgccg cgaattttca gcccccacgc catgttttct
    21301 tcgacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat cccgcgatct
    21361 ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc ggtcaccctc
    21421 tccagcccgg caaccatacg cagcagcgtc gatttcccgc agccagacgg cccgaccatc
    21481 acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgaccta ggttttgcca
    21541 tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct cactatcgac
    21601 caggccgcgc acgaaggcac gctgcatcac taaaacaatc actactggtg ggatcagggt
    21661 taacaacatc gccgccatca ctgagttcca ttccgtggtg ccttcgccag tggcgatcat
    21721 ccctttgatc cctgccacgg tggtgccgag atccacgtcg gtaataatca acaacggcca
    21781 caaatactgg ttccagccgt aaataaaggt gatcacaaac agcgccgcca gattagtttt
    21841 ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt gacgcaccgt cgatccgcgc
    21901 cgcttccacc agttcatccg gcagcgtcat aaagaactgg cgaaacagaa acgtggcggt
    21961 cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcatct tcaggttggc
    22021 gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg tgataaaaat
    22081 catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa atgccgagag
    22141 catcgagaca gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc tgttaagcaa
    22201 catccgccag aacggcgcgc tatttgtgcc taccccgttc acccagatgt tgtggatgtt
    22261 ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg gcgtagacgg cctgtttatc
    22321 cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga tcccgaggat
    22381 cagcatggta tggctgaata tcgtcagcca cggacggttc tcaattattg gtaacgcacc
    22441 ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa catcaacacc
    22501 accgactgtg cggcagacga agccagatcc agtccggtaa aaccttcgcg gtagatctta
    22561 taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg tggcggcgtc gatcaccggg
    22621 aaggtgtcga agaaggcata caccaggttc actaccagca ggaagaaact caccggggcg
    22681 ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc ctgcaccgtc gatggctgcg
    22741 gcttcgatca acgaacgggg aatggattgc agcgcggcat agaagaacag aaagttgtag
    22801 ctgatttgct tccacaccga ggcaaacacc accagaaaca tcgcctgacc gctattttgc
    22861 gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc gcgaccgggg
    22921 ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata cggcagcaac
    22981 attaaggttt ggtagaaacg actgccgcgc acgatgtact ccaccagcgc cgcaaagaac
    23041 agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt cgtccagaag
    23101 gagtcgagat aatagctgtc atgaaacagc gtgacgaagt tatccagacc gacaaactgg
    23161 ctggagaaac caaacggatc gacgctttgt agcgagtacc acaacgcttc gcccgcaggc
    23221 cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata aggcagccag
    23281 cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac tgtgccggat
    23341 gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac gcatccggca
    23401 ttacactgat taagacttcg tcgatttctc aaagcggcgc agtaactggt tcccacgctc
    23461 aactgcggta tccagcgcct gctgtggcgt cttcttaccg gtccacacgc tctcaagctc
    23521 ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgaagccctt tggtgaacgg
    23581 caacggcggc ttgttcagca tctgacgcgt cgcggtatcc gcacctgggt ttttctcgta
    23641 aaagccctgc tcacgggtca ggtcatacgc cgctttggta attggcagat aaccggtttt
    23701 ctgatgccac tcagcagcgt tttctggctt cgcgaggaag tcgagaaact tcgccacgcc
    23761 ggtatacgtt tctttatctt tgccctgcat cacccacagg ctggctccgc cgataatggc
    23821 gttttgtggc gcatctttcg catcggcgtc gtaaggcatc atacctacgc cgtagttaaa
    23881 tttggcgtac tcgcgaatgt tggcaagaga accggaagag gcggtggtca tcgcgcaatc
    23941 accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga agtcgccctt
    24001 cttgttcatc tcctcgagca tggcgatgtg tttcacctgc tccggcttgt tgaactccag
    24061 caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac cgttccaggc
    24121 gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc acttaatacc
    24181 ggaggctttc agttttgcgg aatagtccgc cagatcctgc caggttttcg gcggctgttc
    24241 cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg gagtcgagct
    24301 gttgaacggc tgggagagta agtggccggt tttgctgtcg gagtagtaac cggaaaccgt
    24361 cggcacaaac tgcgactcat cgaactgaat ccctgcctct ttaaacacgt catacaccgg
    24421 tttaatggct ttcgacgcca tcatggtggc ggtgccaact tcataaacct gcaaaatagc
    24481 cggagcgttg ccggtacgaa atgcggcaat ccccgcgctt aaattctgtt cgtagttgcc
    24541 tttataggtc ggtacaattt tgtaatccgg attttcggcg ttaaaacgtt gggccagaga
    24601 atccacctct ttacccagtt ccccttccat agaatgccag aacggaatgg tcgtcactgc
    24661 ctgtgcattc cccattaacg ccagtccgag cgccagtgct gaagctgtat aatgtaacgg
    24721 tttcatcgtt tatctctctt gttgtaccga atgcgcgaat tcacgcgttt tatgctcgcg
    24781 gggtaacatg acatgctcga attacagaaa aataactttt ttgttacatt tgtaagatag
    24841 taaggtgtca gaaagatgac aaggcggtga cggcgtgggt gagggaaaat gggagatggg
    24901 gcacggataa gcggaaaaat agaaggtctg aatcaaactc tacagattac tcatcgtttc
    24961 atgccggatg cggcgtaaac gccttatccg gcctacaaga tcgtgcaaat tcaatatatt
    25021 gcaactcacc cagtaggcct gataagcgca gcgcatcagg caattttgca tttgtcacct
    25081 gtctcaaagg agtcttttga ctccttatca atcaacgtgt tattacccgc ctaaatacga
    25141 acttctcact gcttcgttcg ccagcagcgc gtcgccggta tcggagagca ccacatggcc
    25201 gttttccagc acgtagccgc gatccgccag ctttagcgcc tggttggcgt tctgctcgac
    25261 gaggaagata gtcatcccct gctcgcgcag ctgctcgatg gtgtcgaaaa tttgctggat
    25321 aataatcggc gcaagaccga gcgatggctc atcaagtaac aacaaacgcg gattgctcat
    25381 cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg gacatggtgc ccgcccgctg
    25441 aacacggcgc tcatgcagac gtggaaacag ttcatacacc cactttatgc gctcctgaaa
    25501 ctggtcacgt tcggcaaaga agccgcccat cgccaggttc tcttccaccg tcatccgcga
    25561 gaagacgcga cgcccttccg ggacaatcgc taccgcttcg cgcatgattt tcgccgtctg
    25621 ccagtcggta atgtctttat catcaaacac aattcgcccg ctggtggcgc gcggatcgcc
    25681 gcataacgtg ccgagcaagg tggttttccc cgccccgttc gcgccaatga gagtgacaat
    25741 ctcgccctga ttgatatgca ggctcacctc atgcagcgcc tggattttgc cgtagtgggc
    25801 gctgactttg tcaaaggaca acatgacttt ttccatctta tgcctcacct aaataggcac
    25861 ggatcacgtc cgggttatta cggatttgct ccggcgtacc gtttgccagt ggcgtcccct
    25921 gattgaccac ataaattcgg tctgaaatcc ccatcaccag cttcatatcg tgttcaatca
    25981 acaagatagt ggtgttgtga tgattgcgca gttcggcaat cagctcatcc agctctttgg
    26041 tttcttttgg gttaagacct gccgccggtt cgtcgagcat taaaatctcc ggctgcgtca
    26101 ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata ggccaggtta cttgcctgac
    26161 ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt ggcggcgcgg tcgagcgctt
    26221 cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc agagaacagc ccggttttca
    26281 gttgctgatg ctgcgccacc agcaggtttt caattaccgt catttcacgg aacagacgca
    26341 catgctggaa ggtgcgcacc acgcccatgc gagcaatttg ctgccccggt aaaccttcaa
    26401 gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt gtagaaaccg gtcaggcagt
    26461 taaaaaccgt ggtttttccg gcaccgttag ggccgattaa cgagacgatt tcctgcgggt
    26521 acagttcaag attgacgttg ttcaccgcca gcaggccgcc gaagcgcatc atcagaccgt
    26581 taacagataa taatggctga ctcatgcctg ctctcctttc gctgcgccgt ttttcagctt
    26641 cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc cagatcatca tcagcaccat
    26701 caaaccaccg agcattaaca tgctgtattc gttgaaatca cgcatcaatt cgcgcgacac
    26761 caccagcaga acagccgcca gaatcactgc aaattgcgag cccataccgc cgagcaccac
    26821 tatcgccagc acaaaggccg attcagcaaa ggtgaaggat tccgggctga caaagccctg
    26881 acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg gcgctgatgg taaaagcggt
    26941 cagcttgata cggcgcgggc ttaagcccag cgaacgacag gcgatctcgt cttcacgcaa
    27001 cgcttcccac gcgcggccca gcggcatccg cagcaggcgg ttaatgacaa acaggcttaa
    27061 caccaccagc agcaacgcca ccaggtagag gaagataacg cgatcggacg gatcgtattt
    27121 caggccaaag aaattactga acgtgtccca gccgccttca cgagcggtac ggctgaactc
    27181 gagtccgaag agtgtcggtt tcgggatctg actgattccg ttcgggccgc cggtgatttc
    27241 agtgttattg agcagcaata tgcgcacaat ttcgccgaaa ccaagggtaa cgatcgccag
    27301 atagtcaccg cgcagacgca gcaccggaaa accgagcagg aaacccgccg ccgctgccat
    27361 cagtccggca atcggcaaac aggtccagaa ccccagaccg taatagtgat tgagcagcgc
    27421 aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc agcaccagca gaccagaaag
    27481 accaacaacc acattcagcc cgagaccgag gataatgtag atcatggtca gagtggcgat
    27541 atccaccgtc ccacgtgaca ccataaacgg ccacgccacc acaagcacca gcagcgccac
    27601 gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg ggcagaataa acttcggccc
    27661 ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa agctggaaga aaaagaccac
    27721 cgccgtgccg ataaacatcc actgccaacg gatgtctgaa gccgtgtcga ccaccagttt
    27781 ggtgccatcc agctccagtt gtacgcccat aaagacgccc gccaggacaa agaacatcgc
    27841 ggcagagagc agtgccattg caatatgcat cggtttcata ctttctctac ctccgggcga
    27901 cccagaatac cggtcggcat caccagcagc accagaatca gcagggcgaa tgagaccaca
    27961 tctttatatt ccgtactcag ataggcagaa gagagcgcct ccgcaatccc taggatcagc
    28021 ccgccaatca tcgcccctgg aatgctgcca atcccaccga gcaccgcagc ggtaaaggct
    28081 ttcatcccgg ccataaagcc gatatagggg ttaatgacgc agtagaactg accgagcagt
    28141 acgccagcca ctgccgccat cgccgcgcca ataacaaagg tcaaagcaat cacccggtcg
    28201 gtgttgatgc caagcaggct cgccattttc agatcttccg cgcaggcacg acaggcgcgc
    28261 cccatacggg aatagcgaat gaaaatcgtc agcgccagca tggcgaggaa ggtgacaatc
    28321 cagatcaccg cctgcatggt ggtaatagag gcagagaagt tttcgctatg gcccaccacc
    28381 cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg aaccttcggt caggctgacg
    28441 tagttttgca ggaagatgga cataccgatt gcggaaataa gcgcaatcag gcgcttagag
    28501 ttacgcaccg ggcggtaagc tacccgttcg atactccagc cgtaggcgct ggcgatgacg
    28561 attgcgccga cgaatcccgc cgccaccagc agccagccgg tatcaatgcc catcatcatc
    28621 agcgcggcga tgatcataaa tgagacgtag ctgccaatca tataaacctc gccgtgggcg
    28681 aagttgatca tgccgataat gccgtaaacc atggtgtagc cgatggctat cagcgcgtag
    28741 gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga aatacaaaaa ctgctcagac
    28801 ataaggtaac ctttctaaac ccgcccgcat tttacgggcg gtgggatgat cacttggctg
    28861 ccgtggatga accgtcggcg tgccactgga agacaccaaa atcaaatccc ttaagatcgc
    28921 ctttttcatc ccagttcagc ggcccaatca cggtgtttgc accgttagct tttaaatctt
    28981 tcaccagcgc cagcggctca tcgctgccgg tacgctcaag ggcagttgcc agagattgca
    29041 ctgccgcgta agtgatccag acataaggcc cggacggatc tttcttgtct gctttcagcg
    29101 catcaacgat gccctggttt gccggatcct ggtcatagcg ttttggcata gtgaccaaca
    29161 tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc gttaccgaca ccttctggcc
    29221 ccataaactg ggttttcagg ccaacggaac gggcttggcg cagcatctgc cccatttccg
    29281 ggtagtaacc gccgtagtaa acgaagtcga tattttcttt tttcaggcgg gcgatcagcg
    29341 cggagaaatc tttctccccg gcggtaatac cgtcgaagaa gacgacgttg gcgttagccg
    29401 ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc atactgttgt ttgtcgtgaa
    29461 tgatggcgat gcgctggggc ttcaccgtct caagaatgta ttttgccgcc gtaggcccct
    29521 gggaagagtc cagtccggca gtacgcataa tgtgttgata accgcgttgg gtcagctccg
    29581 ggttggtcgc tcccggcgag atcatcagaa taccttcgtc ttcgtagata tccgacgctg
    29641 gctgggtaga ggaagaacac agatgaccaa taacgtattt aatgccgtcg ttaacgattt
    29701 tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc gtcatattcc acgccaacca
    29761 gcttgtcgcc cttaattccc cctttgacat taatgtcttt aatcgcctga cgcgcgccgt
    29821 taaattccat atcgccccac tgggcaatcg ggccggacat cgcgccgaca acggcgactt
    29881 taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc aatcatccct gcgatgatag
    29941 ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg atgttgtgtt gctttgtttt
    30001 tatgtgttaa caaatcagac tgttcttttt ttatactgca ctgtttttgc ctgtctgatt
    30061 aaggggttag cgcagtattt tgtgatgata gcgattaaaa tccctatttt tcagtcgatt
    30121 aagaacagat aatattctga atttattgat agataaacag aaaaaagtgc ctttgtcagc
    30181 ataaaataac ggcacaaagg gcggaataat tcactatcat tcaggggatt atgctggaca
    30241 tttttcattc tctaatgttt taactttgta attattgctg ttaaaaaatt aatcacctgc
    30301 caaaagaaat aaaaaagaga aagcctccga ttaaattatt tcgctacact ggttccactt
    30361 ttgtgattta cacgggttac ccatgaagct gaccatcatt cgattagaaa actttagcga
    30421 ccaggaccgg attgacctgc aaaagatctg gccggagtat tccccttcct ctttacaggt
    30481 tgacgataac caccgtatct acgccgcgcg ttttaacgag cgtctgcttg cggccgtgcg
    30541 ggtaacctta agcggcaccg agggagcact ggattctcta cgcgtgcggg aagtcacccg
    30601 ccgtcgcggt gtggggcaat atctgctgga agaggttttg cgtaacaatc ctggcgtttc
    30661 atgctggtgg atggcggatg caggcgtgga agatcgcggt gtgatgacgg cgtttatgca
    30721 ggcactggga tttacggcac agcagggcgg ctgggagaag cgttaatcgt caagttgatt
    30781 tcaaaagtga tgaccaacga gaaattgcct gatgcgctac gcttatcagg cctataatgt
    30841 gtgctgcaat ttactgattt ctttggttct tgtaggccgg ataaggcgtt tacgccgcat
    30901 ccggcatgaa gcaacgtatt cgatattagc aatttggcgg caacccaaag ttgccgatta
    30961 acaattactt cgcatcggtc gccgtgccgt tggcgtgcca gtcaaatacg ccgaactcaa
    31021 agccctttag atcgcctttc tcatcccagg tcagtggccc catcacggta tccacggagt
    31081 tcgcttttag gtatttggcg atttcagccg gatcgtcaga ctgattcagg cccgcctgca
    31141 aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc gcttgggtcc tgttttttcg
    31201 ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg atcgtagttc ttcggcttgg
    31261 tcaccagcag cccttccgct gattcgcccg caatgttaga cagcgaaacg ttagccacac
    31321 cttccggccc cataaactga gttttcagcc ctgccgcgcg tgcctgacgc aggatttgcc
    31381 ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat attctctttt ttcagacgcg
    31441 ccaccagcgt tgagaaatct ttttccccgg cggtgatacc atcaaagaac accacgttag
    31501 cattgccttt cttcaggccg tcctgcaccg ctcgtgccag accttcgccg tattgctgtt
    31561 tgtcgtgaac gatagcaata cgctgcggtt tcactttctc aagaatatat ttcgccgccg
    31621 tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat cagctgatag ccacgggcgg
    31681 tcagctccgg cgcggttgcc gctggggtga tcattaaaat gccttcgtct tcgtagatgt
    31741 cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat cacatattta ataccgtcgt
    31801 taacgacttt gttcgccacc gcaaccgcct gtttcgggtc acaggcatcg tcatatttta
    31861 cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt gatatccgca accgcctgct
    31921 ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg accggacatt gcacccacga
    31981 ccgcgacttt aatatcttct gccagagcca tattgctgaa tgccagcgcg atacatcctg
    32041 ccagtaacgc tttacccttt atgttcatcc tgagaatccc cattcttctg gttattacgt
    32101 gtgttgtgat gttgttgttc agcactttat ttcgttttat gcatgactac ccgtgcttta
    32161 gcagcatact ctgctaaaac atacccgatt tttatgatat tggaatagct attttgacag
    32221 tttattaaca atctgcgtgg ggattggcgt tttgccggag ggggaattga ttattgtaga
    32281 ggcccaaaaa acaaaaaccc cggactctca tccagggttc tctgcttaat agcggaaatt
    32341 acgcttcaat agcggcgcga agttttttca tcgcgttctt ttccagttgg cgcacacgtt
    32401 cagcggaaac accgtaacgg tcagccagtt cctgcaacgt ggacttgttg tcttcgtcca
    32461 gccagcgcgc acggatgatg tcctggctgc gttcgtccag accatgcatc gcgtcggtca
    32521 gacggtttgc cgcctgctct tcccagttat catcttcgat gccgtcggca aagttagatg
    32581 atttatcctg cagatagagc accggtgcca tcggctggct gtcggaatcg tcgtcggaag
    32641 acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc catctcacgt acgtctttgc
    32701 tggttacgcc cagttcacgg gccaccattt cgacttcatc ctggttaaac cagcccagac
    32761 gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg cgctttggtg gtcgcaactt
    32821 tgacgatacg ccagttacgc agaacgtatt cgtggatctc tgctttgatc cagtggacgg
    32881 cgaaggagac caggcgcaca cccacttccg ggttaaaacg gcgcactgct ttcatcaggc
    32941 cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc atagcccgca taattacgag
    33001 caatatgaac aacaaaccgc aggtgagaca ggatcagcgt tttagctgct tccagatcgc
    33061 catggtaatg cagcttttca gccagcgccc gctcctcgtc agccgacaac atcggccacg
    33121 cgttagctgc ccggatgtag gaatccaggt tgccaactgg ggctaaagct aaactttgca
    33181 ttttgtcagt cattcaaatc ctctcaatcg atatcttctg gcgcttcagt ggtagcaaca
    33241 actgtgccaa agcttaagag caacgaggtt atcattcact gttttatcag accgtgattt
    33301 tatccacaag ttcaatgcaa gcttgtgaat aaattacgca caaaatgtga catagagatg
    33361 aaataccggg aagagacaac ggggtctctt tccctgctac ggaacccatt gcagggaaag
    33421 agtataacac gcttttatta ttcaggcgta aagtggcgta aatgttgtac cgtggcaagc
    33481 cacgctgcca cccagccaat catcgagcat accagcagca atagcaggca ttcatcgaac
    33541 gataaaccat tgatatcaaa cttcgttccg aaaacctgtg ccacttccgc caccgctgac
    33601 gacaaacgca gtaccagaat ttctgacaaa attaatgaca acaatgcgcc agaaaatccc
    33661 agcagcgcac caccgtacag gaacgggcga aggatgaaac catccgtcgc accaatcagt
    33721 ttctgcacgt tgatggagtc acggcgagca aagatgctca gacgtacgct gttgccgatg
    33781 acgaggaaca ctgccgccac catcaacacg ccgatcatcg ccgaaacgcg cccaaccagc
    33841 ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca tccgcacttc gtcaatgccg
    33901 ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt ccgtcccctg aaaatcgagt
    33961 ttcgggatca ccaccgccac tgccggaagc gggttttctt ccagcatatc cagcgcacca
    34021 ccaaaaccag accagttacg gaactcgccc agtgcgtctt cacgagaaag atagttcact
    34081 ttctccacgc cttgctcggc ctgcaactgt gccaccacgc ccgcagcggc gtcatcgtcc
    34141 agcgtttttt gcaagtaaac agtgatttgc ggtgacggat aatactgcgt cgccgcctga
    34201 ttaacgtttt tgtacaccat ataacagacg ctgggcagcg tcagggagat ggcgataacc
    34261 atcaccgtta aaaacgtggc gaacggtttg cttttcagat cctgcaatgc gccgtggaag
    34321 gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat ttaccggttt tggcgaggat
    34381 ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc cgccgaccga tttacggaag
    34441 cgatccagac gcccaccaaa ctgccgaata tgattgattg catcgcgctt attcatggcc
    34501 cacgcctcca tgcaagtgac catcgctcag ggtgagcatg cgataggaac gccgtgaaat
    34561 caggttaatg tcgtgcgttg ccatcaatac ggttacccca acgcggttaa attcttcaaa
    34621 cagacgtaga atcccttccg acaacgcgtc gtccaggtta ccggtcggtt cgtccgccag
    34681 cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca acacgctgtt gttcaccgcc
    34741 ggaaagctga atagggaagt tcttcgcttt gtccagtagc ccgactttat ccagtgccgc
    34801 cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg ataatcagcg ggatcgccac
    34861 gttatcgtag acagtacggt ccatcagtag atggtgatcc tggaaaatca tgccaatctg
    34921 gcggcgcaga aacggaactt cacggttttt cagacgcgtg atgtcatggc cgctaaacca
    34981 gattttcccg gcgctgggcc gctcaatccc acagatcagc ttcaggaggg tacttttccc
    35041 tgcgccggaa tgaccggtca gaaacgccat ctcacccggc tgcatatgga acgtaacgcc
    35101 ctgcagcgcc tgtctcccac cgagataagc cttgctgaca tgttcaaagc gaatcattgt
    35161 taatcctctc gggcaaaaag tgcctctata aagtcgtccg ccttaaacgg acgcaaatcc
    35221 tcaatacgtt cgccgacacc aatgtagcgg atagggatac caaactggtc agccaccgag
    35281 aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta gcgtgatgcc ggttaagcca
    35341 acagcttcgt ggaacagttt ggcctggctt accgcgttct gcccggtgct ggcatcaata
    35401 gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct tcatcacgcg gacgattttc
    35461 ttcaactctt ccatcagatg cgatttgttc tgcagacgtc cggcggtatc ggcaatcagg
    35521 acgtcgatat tacgcgcttt agctgcctga atggcgtcga agataacaga ggcggaatcc
    35581 gcaccggtat gctgggcaat caccggaata ttgttgcgct gaccccagac ctgaagctgt
    35641 tcaaccgcag ctgcacggaa agtatcgccc gccgccagca tcaccgattt accctgctgc
    35701 tcaaactgac gcgccagctt accaatcgtc gtggttttac ccacaccgtt gacgcccacc
    35761 atcaggatca cgaacggcgt tttatcttca acattcagcg gctcatcgac tttcgccaga
    35821 atctcaccca tctcttcttt cagcaggcca tagagcgcct cggcatcacg aagctgcttg
    35881 cgggatgcgc cttccgtcag attggtgata attttacgtg tggtttccac gcccacatcg
    35941 gcgatcaaca gctgttcttc cagctcttca aacagatcat cgtcgatttt tttaccgcgg
    36001 aacaggctga taaatccgga accgagattt tctttggttt ttaacaggct gcgtttcagg
    36061 cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct gggcaatttc ttcaaccggc
    36121 tgttcttctt ctgccggagg aactaccatc accgcctctt ctgccgcttc ggcagccagc
    36181 gcctgtgctt ccagctcttc gtcggtgatt tcttctttag ccgcttcttc ttctgccgct
    36241 tcgacaatct ctacggtttc cgcttcagcc tgccactctt ctggcgaaac ctcttcggcg
    36301 ttgacgtctt ccggcaacgg cagctcttca cgttcgatag ccactggctc cggcgtttct
    36361 tctacgaccg gttccggctg tgcaacaact tccgcttcag gctgcgcttt ttcactttca
    36421 acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag tttccgcctc ggcttcagta
    36481 tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct tcgcaggctc ttgcgcctga
    36541 acgatttctt ctacaaccgg ttgttcattc tgaacttctg tctctttttc cggggtctgc
    36601 tctttttgac caaagcccag ccaggaaaaa aagccacgtc ttttttcttt cgccatttgc
    36661 gactacactc ctcgctgttg cttcatggca cagcgttaac gctatgtaca tggcagctaa
    36721 aaaaatgatg aaatagtcta tcacttaact taattcacat caccgcctgc aagtatatgt
    36781 tatctggcgg attgagcaat ttatcatgaa aaaaccgaat cattccggca gcggccaaat
    36841 ccgcattatt ggcgggcagt ggcgaggccg taaactcccg gttcctgata gcccaggtct
    36901 gcgccccacc accgaccgcg tacgcgaaac gttgtttaac tggctggctc cggtcattgt
    36961 tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg ctggggctgg aagcgttatc
    37021 gcgctacgct gcgggggcaa cgttgattga gatggatcgc gtggtttctc agcagttaat
    37081 taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg gtgaacagca acgcaatgtc
    37141 attcctggcg caaaaaggca caccgcataa tatcgtgttt gtcgatccac cgttccgccg
    37201 tggcttgtta gaagagacga taaatttact ggaagataac ggctggctgg ctgacgaagc
    37261 cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc actgttccgg caaactggtc
    37321 attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg ctgtatcaac acgaagcaca
    37381 aggagaaagt gatgctgatt aatattggtc gtttgttaat gctctgcgtt tggggatttt
    37441 taatcctcaa tctggtgcat cccttcccgc gcccgctaaa tattttcgtt aacgtggcgc
    37501 tgatttttac cgtgctgatg catggtatgc agctggcgct attgaaatcc actttaccga
    37561 aagatggccc gcagatgacc accgccgaaa aggtgcggat tttccttttc ggcgtgtttg
    37621 aactgctggt ctggcagaag aaatttaaag ttaaaaaata accttattgt tcgccaacaa
    37681 agctaacaaa gcgcgttcct ttatagctca gcgtaccttt atcgcccacg gtcagggcgt
    37741 ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc tccgctttgc ggtttgaagc
    37801 ttgcctcata gcgcatactg gtgcctgccg gagtcacttc ctgctggcgc gaacggcgat
    37861 cgttaatcgg tttttcccgc ttgttgctca ccaccaccag cttttgctgg agcggagcca
    37921 tatcattatc agctttttcc cgtcgctgtt gcataaaacg aaacgatgcg gcgacgacaa
    37981 ttaagccaat gataacaata aagaaaagag gtggtttact catctttatc cctcatcgga
    38041 aaatgcggaa ttaagcatac cctgccagtt atggtgttgt catccgtcca ccctcgccac
    38101 taaactggaa gcaagaccgt aggcattccg cttacgaaaa aataacgaat tcaaggaact
    38161 aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat ggctatctgt ggatgcatcg
    38221 tatcgtgggc caacctggca acgctgggtg tttaaaccgt taacccttct tctcctgctg
    38281 ttactggcct ggcaagcacc gatgttcgac gccattagct atctggtgct ggcgggactg
    38341 tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac gtcaacgtct gatgtacgcc
    38401 atcggcgcgt ttttcctctc gcacctgctg tacaccatct atttcgccag tcagatgacg
    38461 ctctctttct tctggcctct gccactggtg ttgctggttc tgggtgcgct gctactggcg
    38521 attatctgga cgcggctgga agagtaccgt tggcctatct gcacgtttat cggcatgacg
    38581 ctggtgatgg tgtggctggc aggtgaactg tggttcttcc gtccgaccgc tccggcgctc
    38641 tctgcgtttg ttggcgcttc gttgctgttt atcagtaact ttgtctggct ggggagccac
    38701 tatcgccgac gcttccgtgc ggataacgcg attgctgcgg cctgctactt tgccggtcac
    38761 ttcctcatcg tccgctcact gtatctctga taaaacttga ctctggagtc gactccagag
    38821 tgtatccttc ggttaatgag aaaaaactta accggaggat gccatgtcga cacctgacaa
    38881 tcacggcaag aaagcccctc aatttgctgc gttcaaaccg ctaaccacgg tacagaacgc
    38941 caacgactgt tgctgcgacg gcgcatgctc cagctcgcca acgctctctg aaaacgtctc
    39001 cggcacccgc tatagctgga aagtcagcgg catggactgc gccgcctgtg cgcgcaaggt
    39061 agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg caggtgttgt tcgccaccga
    39121 aaaactggtg gttgatgccg acaatgacat ccgtgcccag gttgaatctg cggtgcaaaa
    39181 agcgggctat tccctgcgcg atgagcaggc cgccgacgag ccacaagcat cgcgcctgaa
    39241 agagaatctg ccgctgatta cgcttatcgt gatgatggca atcagctggg gtctggagca
    39301 gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc acgctagttg ggctgtaccc
    39361 gattgctcat caggcattac ggctgatcaa atccggcagc tacttcgcca ttgaaacctt
    39421 aatgagcgta gccgctattg gtgcactgtt tattggcgca acggctgaag ctgcgatggt
    39481 gttgctgctg tttttgattg gtgaacgact ggaaggctgg gccgccagcc gcgcgcgtca
    39541 gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc acgcgcctgc gtaacggtga
    39601 gcgggaagag gtggcgatta acagcctgcg gcctggcgat gtgattgaag tcgccgcagg
    39661 tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt gccagttttg atgaaagcgc
    39721 cctaaccgga gaatccattc cggtggagcg cgcgacgggc gataaagttc ctgcaggagc
    39781 caccagcgta gaccgtctgg taacgctgga agtgctgtca gaaccgggtg ccagcgccat
    39841 tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc cgcgccccca ttgagcggtt
    39901 tatcgaccgt ttcagccgta tttatacgcc agcgattatg gccgtcgccc tgctggtaac
    39961 gctagtgccg ccgctgctgt ttgccgccag ctggcaggag tggatttata aaggtctgac
    40021 gctgctgctg attggttgcc cgtgtgcgtt agttatctca acgcctgcgg cgataacctc
    40081 cgggctggca gcagcagcgc gtcgtggggc gttaattaaa ggcggtgcgg cgctggaaca
    40141 actaggtcgt gttacccaag tggcgtttga taaaaccggt acgctgaccg tcggtaaacc
    40201 gcgcgttacc gcgattcatc cggcaacggg tattagtgaa tctgaactgc tgacactggc
    40261 ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa gccatcgtac gcgaagcaca
    40321 ggttgctgaa ctcgccattc ccaccgccga atcacagcgg gcactggtcg ggtctggcat
    40381 tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct gccgggaaac atcccgctga
    40441 tgcatttgct ggtttgatta atgaactgga aagcgccggg caaacggttg tgctggtagt
    40501 acgtaatgat gacgtgctgg gtatcattgc attgcaggat accctgcgcg ccgacgctac
    40561 aactgccatc agtgaactga acgcgctggg ggttaaagga gtgatcctca ccggcgataa
    40621 tccacgcgca gcggcggcaa ttgccgggga gctggggctg gagtttaaag cgggcctatt
    40681 gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa catgcgcccc tggcgatggt
    40741 cggtgatggt attaacgacg cgccagcgat gaaagctgcc gccatcggga ttgctatggg
    40801 tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca ttaacccata accacctgcg
    40861 cggcctggtg caaatgattg aactggcacg cgccactcac gccaatatcc gccagaacat
    40921 caccattgcg ctgggactga aagggatctt cctcgtcacc acgcttttag ggatgaccgg
    40981 gttgtggctg gcagtgctgg cagatacggg tgcgacggtg ctggtgacgg cgaatgcgtt
    41041 aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca ttgagcgcga tcggtcccct
    41101 cgcccctctg gggagagggt tagggggtga ggaaaaggcg gcatcgaagc caatcagccc
    41161 ctatcaaccg cctttacgaa tcaaataacg ataaggcagt ccatccgtct ctttcgcaac
    41221 cagttcgtgt tccataaagg tacaaaaccc aggaatatcg cgggtagtgg ccggatcgtc
    41281 ggcgataatc agcaacgttt cgccaggctg catattgcgc acggttttgc gcaccatcat
    41341 caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg tggtcagggc tggaaaagag
    41401 atcggtcatt ttcttctcat cacttaaaaa aacggcgcta gtttacgccc tgtgagtccg
    41461 taagcaaacc aggttaacga ttgcgtgaaa attaaccatt gcattgtcaa cgtaaagcag
    41521 tatcatgcgg cggctcgaaa aaagggtaag cacgttatta tgttaaggta acagacgtgt
    41581 cgtacgtatt gggttccctc accccaatgg ttaatcaaaa aggtacaata tgaacgtttt
    41641 ctcgcaaact caacgctata aggcgttgtt ctggttatcg ttatttcatc ttctggtgat
    41701 cacctccagt aactatctgg ttcagcttcc cgtctccatt tttggtttcc ataccacctg
    41761 gggcgcgttt agctttccgt ttatttttct tgctaccgac ctgaccgtgc gtatttttgg
    41821 cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc cctgcgttat taatctccta
    41881 cgtcatctcg tcgctattct atatgggttc ctggcaggga ttcggcgcac tcgcccactt
    41941 caacctgttt gtcgcccgta tcgccaccgc cagtttcatg gcctacgcgc tggggcaaat
    42001 cctcgacgtg cacgttttta accgcctgcg tcagagtcgc cgctggtggc tggcaccgac
    42061 agcgtccaca ctgttcggta acgtcagcga cacgctggcc tttttcttca ttgccttctg
    42121 gcgtagcccg gatgccttta tggctgaaca ctggatggaa atcgcgctgg tcgattactg
    42181 tttcaaagtg ttaatcagta tcgttttctt cctgccaatg tatggcgtat tactcaatat
    42241 gctgttgaaa agactggcag ataaatccga aatcaacgct ttgcaggcga gttaaaggtt
    42301 cgtaatcaga gttgtgataa gatggatgaa tgagccgtta tggccgttta tcgaaaggaa
    42361 gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc ctgctggtta tggggcttgc
    42421 ggcctgtgat gataaagaca ctaacgctac ggcgcagggt tcggtcgcgg aaagtaacgc
    42481 taccgggaat cccgtcaacc tgcttgatgg caagttaagt ttctcgctgc cagcggatat
    42541 gaccgaccag agcggtaagc tgggaacgca ggccaataac atgcatgtct ggtccgacgc
    42601 caccgggcag aaagcagtca tcgtcatcat gggcgatgat ccgaaagaag atctggcggt
    42661 gttggcgaag cgtctggaag atcagcaacg tagccgcgat ccgcagctgc aagtggtaac
    42721 caataaagcc attgagctga aaggtcacaa aatgcagcag ttagacagta ttatctccgc
    42781 gaaaggccag acggcgtact cttccgttat tctgggtaac gtgggtaatc aactgctgac
    42841 catgcaaatt acgctgccag ctgacgatca gcaaaaagcg caaaccaccg cagaaaacat
    42901 cattaatacg ctggttattc agtaagtttt aagatgatga ggcggcctca gggacgtgtt
    42961 ccggaggccg tttttttaat cgccacgtca gtaataacgc gattgcgacc agtcccgccg
    43021 ccgccagata aatcaccggt acgcccgccc agctcatcac cagcccagcc agtggtccag
    43081 tcacgccaag cgataaatcc ataaatacgg tgtaagttgc cagcgccgcc ccctaatttt
    43141 gctgcggaac cgcttttacc gccactacac ccaatgccgg gaacaccagc gaaaacccgg
    43201 cccccgccag taagacgccg attttcgcca tccacggcat agtcgccacg ccaaccagta
    43261 gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac gtttaagcca ccgatacggt
    43321 taatgccgtt agggaataac aaacgcgtac cgacaaacgc acagctaaac agcgtcagcg
    43381 cgaaagccgc accgtcccaa cctttagcgt cataaaacag cgtgataaag gtggcgatga
    43441 cgccaaatcc ggcggaagcc agtgccagcg ccataccgta cagccagacg cgcccaagca
    43501 ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac cgtcggacgc gggatcgcca
    43561 acaaaatggc caccagcgcc acgcccataa tgattaacgc taacgcctgc aagccgcccc
    43621 agtgataaaa cacgacgcct aacggcgcac ccatcgccat cgccccgtaa gtgacaatgc
    43681 cgttccacga aatcacccgc ccgatatgca gcgagccaac cactccaacg ccccataggg
    43741 tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat gacgcgcccc aggcaaagta
    43801 ataacaggct gatgacaggc agactggcgg ttaatcctgc cgtcagatac cccagaccgc
    43861 tcaaaaagca gccgcataaa ccgaagacga caatcttttt gggtcccagc aaatcggcgt
    43921 aacgtccggc atgagggcgg ctcagcaagg tggcgaaata ttgcaggctg ataaccaatc
    43981 ctgcccagaa ggcgctaaag cccatcacat catggacata gcccggtaat acagcgagcg
    44041 gcaacccgat ggtgaggtag ctggcgaagt taaacatgac aatggagaca atgcgcaaat
    44101 tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc gggcatgagg atcaccacat
    44161 ttttacaaca gtgtttcatt tttaccatgt gctgacgtga aaatcagcag taagaatcag
    44221 aatattgctg gcgtgactcc cgctacactt aacacaaaaa gtcacaagga agccccaatg
    44281 gaaacccctc aacccgataa aacgggcatg cacattctgc tcaagttggc ctcgctggta
    44341 gtgatcctcg cgggcattca cgcagcggca gatatcattg tgcagctgtt actggcgctg
    44401 ttttttgcca tcgtcctcaa cccgctcgtt acctggttta ttcgtcgggg agtacaacgc
    44461 cccgttgcca ttacgattgt agtggtggtg atgctgatcg cactaaccgc gctggtcggc
    44521 gtactggcgg catcgtttaa cgaatttatc tctatgctgc cgaagtttaa taaggagctg
    44581 acgcgcaaac tttttaaatt gcaggagatg ttgccttttc ttaatttgca tatgtcgccg
    44641 gagcgaatgc tgcagcggat ggactcggaa aaagtggtta ccttcaccac agcgctaatg
    44701 accgggcttt ccggggcaat ggcgagcgtg cttttgctgg tgatgaccgt agtttttatg
    44761 ctgtttgaag tgcgccacgt cccttacaaa atgcgttttg cgctgaataa cccacagatt
    44821 cacatcgcag gattacaccg cgcacttaaa ggcgtttcgc attatctggc gttaaagacg
    44881 ctactcagtt tatggacagg tgtcatcgtc tggctggggc tggcgctaat gggcgtacag
    44941 tttgcgctga tgtgggcagt actggcgttt ttgctcaact acgtgcccaa tatcggcgcg
    45001 gtaatttccg ccgtaccgcc aatgattcag gtgctgctgt ttaatggtgt ttacgaatgt
    45061 attctggtcg gcgcattgtt tttagtggtc catatggtca tcggcaatat tttagaacca
    45121 cggatgatgg gccatcgcct ggggatgtcc accatggtgg tatttctttc attgttaatc
    45181 tggggatggc tgctcggccc ggtagggatg ctactttcgg tgccattaac cagcgtgtgt
    45241 aaaatctgga tggaaacgac gaaaggtggc agcaaactgg cgattttact ggggccggac
    45301 agaccgaaaa gtcggttacc gggatgaggc gacaagtgat acgatacgca ctttcatttt
    45361 ccattaaacg ttggccctga tatgtatcgg atagttctgg ggaaagtttc gaccttaagc
    45421 gcagctccac tgccaccggg tttacgcgag caagcaccgc aaggtccacg acgcgaacgc
    45481 tggctggcgg ggcgtgcatt gctttcgcac acgctttccc cgctaccgga gatcatctat
    45541 ggcgaacaag gcaaacctgc attcgcgccg gaaacgccgc tatggttcaa cttaagccat
    45601 agcggtgacg atatcgctct gctgttgagc gatgaaggcg aagtcggctg cgatatcgaa
    45661 gtgattcgcc cgcgcgccaa ctggcgctgg ctggcgaacg ccgtgttcag cctcggggaa
    45721 cacgctgaaa tggacgccgt gcatcctgat cagcaactgg aaatgttctg gcgcatctgg
    45781 acgcgcaaag aagccatcgt taaacagcgt ggcggcagcg cctggcaaat cgtcagcgta
    45841 gatagcacct atcactcctc gctgtcagtc agccattgtc agctagaaaa tttaagcctt
    45901 gcgatctgca cccccacccc ctttacgctc accgccgaca gtgtgcaatg gatcgattca
    45961 gttaactgat ccgcccaccc gactgcccat ctattgatcc agaacaggta atcagtatga
    46021 cgaatactta aaatcgtcat acttatttcc gccatctatt ttaatccatt ggggttacca
    46081 tgctctccac actccgccgc actctatttg cgctgctggc ttgtgcgtct tttatcgtcc
    46141 atgccgctgc accagatgaa atcaccaccg cctggccggt gaatgtcggg ccactaaacc
    46201 cgcaccttta cacgcctaac cagatgttcg cccagagcat ggtttatgaa ccattggtga
    46261 aatatcaggc agacggttcg gtgatcccgt ggctggcaaa aagctggact cattcagaag
    46321 atggtaaaac ctggaccttc accctgcgtg atgacgtgaa attctccaac ggtgaaccgt
    46381 tcgatgccga ggcggcggca gaaaacttcc gcgcagtgct cgataaccgt caacgtcacg
    46441 cctggctgga gctggcaaac cagattgttg atgttaaagc actcaataaa acagagctgc
    46501 aaattaccct gaaaagcgcc tactatcctt tcctgcaaga actggccctg ccccgtcctt
    46561 tccgttttat cgctccctcg cagtttaaaa accatgaaac catgaacgga attaaagcgc
    46621 cgattggcac cggaccgtgg attttgcagg aatcgaaact gaatcagtac gatgtcttcg
    46681 tccgtaacga aaactactgg ggcgaaaagc cagcgattaa aaagatcacc tttaacgtca
    46741 tcccggaccc gactacccgc gcggtggcgt ttgaaactgg cgatatcgac ctgctgtacg
    46801 gaaacgaagg gttattaccg ctcgatacct tcgcccgctt tagccagaat ccggcttacc
    46861 acacccaact gtcacagccg ctcgaaaccg tgatgctggc gctcaatacc gccaaagccc
    46921 ccaccaacga gctggcagta cgtgaagctc ttaattacgc ggtaaacaaa aaatcgctga
    46981 ttgataacgc gttatatggc acccagcagg tcgccgacac cctgtttgcc ccttctgtgc
    47041 cttacgctaa tctcggcctg aaaccgcgcc agtacgatcc gcagaaagcg aaagagttgc
    47101 tggaaaaagc cggttggacg ctgcctgcgg gcaaagacat ccgcgagaaa aatggtcagc
    47161 cgctgcgcat tgaactgtcg ttcatcggca ccgatgcgtt aagcaaatcg atggcggaaa
    47221 tcattcaggc tgatatgcgc cagattggcg cagatgtctc gctgattggc gaagaagaga
    47281 gcagtatcta tgctcgtcag cgcgacggtc gttttggcat gattttccac cgcacctggg
    47341 gcgcgccata cgatccacac gccttcctga gttcaatgcg cgtaccgtcg cacgctgact
    47401 tccaggcaca gcaaggatta gccgacaagc cgctgattga taaagagatc ggcgaagtgc
    47461 tggcgaccca tgacgaaacg caacgtcagg cgctgtatcg cgacattctg acccgtctgc
    47521 atgacgaggc ggtttatctg cctatcagct acatctcgat gatggtggta tcaaaaccgg
    47581 agctgggtaa tatcccctac gcgccgatcg ccaccgaaat tccgttcgaa cagattaaac
    47641 cggtgaaacc ttaatgttgc gttacgtatt acgccgcttt ctgctgctga tcccgatggt
    47701 gctcgccgcc tcggtgatca tttttctgat gctgcgcctc ggtaccggcg acccggcgct
    47761 cgattatctg cgtttatcta acctgccgcc gacgccggag atgctggcct ctacccgcac
    47821 catgctcggg ctggatcagc cgctgtacgt ccagtacggt acctggttgt ggaaggcgct
    47881 gcatcttgac tttggtatct cattcgccag ccaacgcccg gtactggacg atatgctgaa
    47941 cttcctgccc gccacgctgg aacttgcagg tgcggcgctg gtattaattc tgctcacttc
    48001 cgtaccgctc ggtatctggg cggcgcgcca ccgcgatcgt ttgccggatt tcgccgtgcg
    48061 ctttatcgcg tttcttggcg tgtcgatgcc caacttctgg ctggcatttt tgctagtgat
    48121 ggcgttttcg gtgtatctgc aatggctacc cgcgatgggc tacggcagct ggcagcacat
    48181 cattttacct gcggtttcca tcgcctttat gtcgctggcg attaacgcgc gactgctgcg
    48241 cgccagtatg ctggacgtcg ccggtcagcg tcacgtcacc tgggcgcggc tacgcggcct
    48301 gaatgacaaa cagaccgaac gccgccatat tctgcgcaat gcctcgctgc cgatgatcac
    48361 cgccgtgggg atgcacattg gcgaattgat tggcgggacg atgattatcg aaaacatctt
    48421 tgcctggcct ggcgtcgggc gctatgcggt gtcggcgatt tttaaccgtg actatccggt
    48481 gattcagtgc tttacgctga tgatggtggt ggtttttgtg gtctgtaatt tgattgtcga
    48541 tttgctcaac gccgcgctgg acccgcgcat tcgtcgtcat gaaggagcgc acgcgtgaac
    48601 tttttcctct cttcccgctg gtcggtacgc ctggcgctga tcattatcgc cctgctggcg
    48661 ctgattgcgc tcaccagcca gtggtggctg ccgtacgatc cacaggcaat tgatttgccg
    48721 tcgtgcctgc tttcgccgga tgcgcagcac tggctgggca ccgatcactt aggtcgcgat
    48781 attttctcgc ggctgatggc agcgacccgc gtgtcgctcg gttcggtaat ggcctgcctg
    48841 ctgctggtgc tgatattagg gctggttatt ggcggcagcg ccgggttgat tggcgggcgc
    48901 gttgatcagg ccaccatgcg cgtcgccgat atgtttatga ccttcccgac ctcgattctg
    48961 tcgttcttta tggttggcgt gctcggcacc gggctgacca acgtaattat cgccatcgcc
    49021 ctgtcgcact gggcgtggta tgcacgcatg gtgcgcagct tggtgatttc actacgccaa
    49081 cgcgagtttg tgctggcgtc acggctttcc ggtgcgggcc atgtgcgggt gtttgtcgat
    49141 catctggcag gcgcggtgat cccttcgctg ctggtgctgg caacgctgga tatcggccat
    49201 atgatgctgc acgtcgcggg gatgtctttc cttggcctcg gtgtgaccgc gccgaccgcc
    49261 gaatggggcg tgatgattaa cgacgcgcgc cagtatatct ggacccagcc gctgcaaatg
    49321 ttctggccgg ggctggcgct gtttatcagc gtgatggcct ttaacctggt gggtgacgca
    49381 ctgcgcgatc atctggatcc tcatctggtg acggagcacg cacactaatg ccgcaacaga
    49441 ttgaactacg taatatcgcg ctacaggccg cgcagccgct ggtacacggt gtatcgttaa
    49501 ccctgcaacg cgggcgcgtg ctggcgttag tcggcggtag cggcagcggg aaatcattaa
    49561 cctgcgccgc gacgctgggc attttgcccg ctggcgttcg ccagacggcg ggggaaattt
    49621 tagccgatgg caaaccggtt tcgccttacg ccctgcgcgg catcaaaatt gccaccatca
    49681 tgcagaaccc gcgcagcgcc tttaatccac tgcacaccat gcacacccac gcgcgggaaa
    49741 cctgcctggc gttagggaaa cccgccgatg acgctacgct taccgctgcc atagaagcgg
    49801 tggggctgga aaacgccgcg cgcgtgctga agctgtaccc gttcgagatg agcggcggca
    49861 tgttgcagcg catgatgatt gcgatggcgg tgctgtgtga atcaccgttt atcatcgccg
    49921 atgaaccgac caccgacctc gacgtggtag cacaggcgcg catcctcgat ctgctggaaa
    49981 gcattatgca aaaacaagcg ccgggaatgc tgctggtcac ccatgatatg ggcgttgtgg
    50041 cgcgtctggc ggatgacgtg gcggtgatgt cacaaggtaa aattgtcgaa cagggcgatg
    50101 tagaaacgct gtttaacgcc cccaaacata cggtgacgcg cagcctggtt tccgctcatc
    50161 tcgccctcta cggtatggag ctggcatcat gaccttactt aacgtctgcg gcctttccca
    50221 tcactatgcc cacggtggat ttagcggaaa acatcaacat caggcggtgc tgaataacgt
    50281 ttccctgacc ctgaaaagcg gcgaaaccgt cgccctgctg ggacgcagcg gctgcggaaa
    50341 aagcaccctc gcgcggctgc tggtaggctt agagtctccc tcacaaggga atattagctg
    50401 gcgcggcgaa tcgctggcga aacttaatcg cgcccaacgt aaagccttcc gccgtgatat
    50461 tcagatggtg tttcaggact ccatcagtgc cgtgaatccg cgcaaaaccg tgcgcgagat
    50521 cctgcgtgaa ccgatgcgcc acctgctatc actgaaaaaa tccgaacaac tggcgcgcgc
    50581 cagcgaaatg ctgaaggcgg tcgatctcga tgacagcgtt ctcgacaaac gcccaccgca
    50641 gttaagcggc ggccagctcc agctcgtctg cctggcacgc gcgctggcgg tcgaaccgaa
    50701 attgctgatt ctggatgaag ccgtttctaa ccttgatctc gtgttacagg cgggcgtcat
    50761 tcgcctgctg aaaaatctac aacaacagtt tggcactgcc tgcctgttca tcactcatga
    50821 cttacgcctg gtggaacgct tttgccagcg agtgatggtg atggacaacg gacaaatcgc
    50881 cgaaacccag gcggtgggag ataaattaac cttttcctct gacgccggac gtgtgctaca
    50941 aaacgcggta ttacccgcat tccccgtgcg ccgtcgcacc tcagaaaagg tttaacgcca
    51001 atgcaacgag tcaccatcac gcttgatgac gatttactgg agacgctgga cagcctgagc
    51061 cagcgtcgtg gttataacaa ccgttccgaa gctatccgcg atattctgcg tagtgccctg
    51121 gcgcaggaag ctacccagca gcacggcaca caaggtttcg cggtgctgtc gtatgtgtat
    51181 gaacacgaaa aacgcgactt agccagccgt attgtttcca cccagcatca tcaccacgac
    51241 ctctccgtcg ccacgctaca tgtgcacatc aaccacgacg actgtctgga aatcgccgtg
    51301 ttgaaaggtg acatgggtga cgtgcagcat tttgccgatg acgttatcgc ccagcgcggc
    51361 gtgcggcacg ggcatttgca gtgcctgccg aaggaagatt gagttatttc taaaacaagg
    51421 aaagaaaaga tattaactga acttaattta taaaattaag ttcagttgca tagatagatc
    51481 ctgtaaaggc agatattctg cgtaatgtac gtcgctaaaa tttataaatg attcaaacgc
    51541 tcattcagtg tttcaataat ataggtatta acagagactt gttgctgcgc agccgcatta
    51601 ttaaggcgtt cactcagcga ttctggatag cgtaacgtaa aggtcttaat cttttctgta
    51661 cgcgcatacg gctcaatacc cgcagcttta caatcctcaa gatactctcg cagggaaagc
    51721 tctccttctt tttgcagccc ctgaatactg tcagagacaa aatcacaata acctgacaag
    51781 cccaggaatt tgccacgaaa tgcattcagt tctggcacgt aggtaataac agctggttgc
    51841 ccggcaatct ccatagagtt tggcgtcttc agtttgatca tggttttacc cctatactta
    51901 gtaaccagtc acgcacgctt tctacagcgc ctttatcggt atctggcgac ggatgaggcc
    51961 gatggaaaca cgcaacgctc atatttagta tgaacttgca acgcgaacct cttccttcct
    52021 taatttctcc gcctaatgct ttaaccagtg actctatatc agaccacttg atcccttgag
    52081 gaacaggtgt tttaaatatt tgatccaatg tatttttttg tttcttccgt aaagcctgta
    52141 ccttggttct catggcatgt ccttatgact tcactttatg acatcacagt atgaagtcaa
    52201 gtttcccttc cttacgccat tgtcccaatc gtcttcctga atcgcagcag tgcgatggtg
    52261 aaaaacgcgc cgccaattgc catcagcgtc aggaactgcg gccagacgat ttcgaatccg
    52321 gcaccccggt agaggatggc ctgcgcgagg ctaacaaagt gtgtcgtcgg catggtcagc
    52381 ataatgtcct gcaccatctg cggcatactt tcgcgcggcg tggatccacc ggaaagcatt
    52441 tgcagcggca gcagcaccag aatcaccagc agccccagtt gcggcattga acgcgctatc
    52501 gtccccataa aaatgccgat tgacgtggtg gcaaacagac tgagcgccac gcccagcata
    52561 aacagcggga tcgagccttc aatcggtacg cccagtacac ctttcaccat cagcaccagc
    52621 gataatcccg ataccaccag caccaccagc cccatcgacc agatcttcgc catcatgatc
    52681 tcaaacggcg ttatcggcat caccagtaaa tgttccaccg tgccgtgttc acgctcgcgg
    52741 atcagcgccg atccggtcaa tacaatcgcc agcatggtaa tgttgttgat gatcgccatc
    52801 accccgccaa accacgcggg atcaaggttc gggttaaagc gcatccgggt ttccagcgat
    52861 accaacggtt cgctgttatc acggtagcgc gcgacaaagc tgttcacttc accgttgata
    52921 atattctgga tatacccatt gccggtaaat gcctggctca tgcgcgtggc atcgacgttc
    52981 acctgaatat ccggctggcg tccggcgagg acatcacgct gaaaattagg cggaatattt
    53041 atcgcgaagg tatagcgtcc ggcgtccagt ccggcatcca tctcatcggc ggtgatcatc
    53101 tccggtggca aaaaccacgg acgatagaag ctgttaacga tccggttcga taactgcgat
    53161 tgatccatat cggcaatggc gatcggcgcg aggttcaacg atcctggcgt aacggtcgct
    53221 gacgaataca ccgacaccgt aaacgagaag acaatcagcg tcagcatcgc tttatcaccg
    53281 agcagactgc gcaactcttt gatacccaga ttaaaaatat tgcgtaactg gcgcatcatc
    53341 cctcctgttt tttcagcagc aggatgctta agcccatcac cagcgggatg gctatcagca
    53401 acgggataaa aagttgccac aaatcagtca gatccagcgc tttcgagaac gttccgcggg
    53461 cgatagtcag aaaatgactg gtcgggtaaa cctcgccgat ccaacgtcca ggcccttcca
    53521 gcgaagctac cggatcgatc atcccggaaa actgtgtcgc cgggatcaat gtgataatcg
    53581 ccgttccgaa aatggcggca atctggcttt tcataaaggt ggagatcagc agccccattc
    53641 cagtggcaat gatgatatac agcagcgccg ccaaggtgag cgtcaggaaa ctgcctttat
    53701 gcggtacgcc aaacacaaac accgacaggc cgcagagcag gaaaaagttc agcatcccca
    53761 gcgcgatgta cggcagctgt ttgccaagca aaaattcgct acgggtggtg ggggtcacgt
    53821 aaaggttgat aatcgaccct agctcttttt cccgcaccac gctaagggcg cttagcattg
    53881 acgggatcat catcagcaga agcgggatca ccgccggaac aatcgctggc aggcttttta
    53941 cgtccgggtt atagcgataa cgcgtctcaa tgttcatcag cccgctttgg ctggcgggtg
    54001 tcgattgtcg gctcgccaca tcctgaagcc aactctggtg cattgcctgc acgtaacctt
    54061 ttaccgtttc ggcacgactg ggcatcgctc cgtcgatcca gacgccgagt tccacaggcg
    54121 taccacgcgc gatatcgcgc ccgaaattgg gcgggatctc aatggccacc gtgatatcgc
    54181 ccgcacgcat ccgacgatca agctcgtcat aactggtgag cggcggctgt tcgataaagt
    54241 aacgggaacc ggagaggttg agtgtccacg cctggctact gacggtctgg tcgcggtcga
    54301 gcaccgcaaa gcgcaggttt tccacatcca tactgatgcc gtaacccatt atcagcatca
    54361 ggatcaccgt tcccatcagc gccagcgtcg aacgtactgg atcgcgtcgc agttccagcg
    54421 cttcgcggcg gctgtagcta aacagacggc gcaggctaaa tccctgacgc ggcgcgtggg
    54481 tggtgtcgtg taccacgggc ggcgcttcgg cttcgttgct ctgccctgcc gcttcctgca
    54541 aataggcgat aaatgcctct tccagactgg cggctccgcg tttctcaacc agttcctgcg
    54601 gtgtaccgct ggcaagcact tttccggcgt gcatcagtga gatgcggtcg caacgttccg
    54661 cttcgttcat aaagtgggtg gagatgaaga tagtcacttt gtcctggcgc gagagatcga
    54721 ccatcaactg ccagaacata tccctcgcca ccggatcgac accagaagta ggctcatcga
    54781 ggattaacat ctccgggcga tgaatcaccg ccaccgccag cgaaagccgc tggcgaatgc
    54841 cgagcggcaa tgactccggc agaacatctt caacgtcgtt gagcttaaaa cgctcgctca
    54901 tttcagccac tcgcgcggga atttccgctt ccgggatgtg aaacaaacgg gcatgtaact
    54961 ccaggttttg ccgcacggtg agttcgttat agagcgaaaa cgcctgcgac atatagccca
    55021 cacgacggcg ggtatcgata tcttttggat caaccggttg cccaaatagc cacgcctcac
    55081 cttcgctggc gggcagcagt ccggtaagca ttttcatggt ggtggattta ccgcagccgt
    55141 tcgaaccaag gaatccaaaa atctccccgc gtggaatgcg gaaattgacg tgatcaacgg
    55201 caacgaagga accaaaacgc atggtcagat cgcgcgcttc gatggcaatc tctgcgtttt
    55261 caggttgata cggtgggatc actaccgcct gatgcgcctg gcgttgcgct tgcggtaaca
    55321 gattaataaa tgcttcttcc agcgtggcgc tttgcgtttg ctgccgcagc tcttcggcgc
    55381 tgccggttgc cagcacttct ccggcattca tcgctaccag ccagtcgaag cgttcggcct
    55441 cttccatata ggcggtggcg accagcacgc tcatattgct ctgccgctgg cgaatactgt
    55501 cgatcagatc ccagaactgg gcgcgggaga gcgggtcaac ccccgttgtt ggctcatcaa
    55561 ggatcaacag ttccgggtcg tggattaacg cgcagcacag cccaagtttt tgcttcatcc
    55621 cgccggagag tttacctgcc ggacgatcgc gaaacggtgc taacccggtg ctggtcagca
    55681 gctcattaat tcgcacttcc cgctccgctt tatcgtgacc gaacaggcga gcgaaaaaat
    55741 cgacattttc atacaccgac aaggtgtggt agaggttttt gcccagcccc tgcggcatcc
    55801 aggcgatgcg cgggcagacg tctcggcgat gcttcgggtc gtgcatatcg ccacccagca
    55861 ccaccacatt gccctgctca atgacgcggg caccggaaat cagtgacaat aaactcgact
    55921 tcccgacgcc atccggacca atcagcccga ccatgcaacg ggccggaata tcgagagtga
    55981 tattgttcag cgcaacggtt tttccataat gctggctcac gcccgccagt tgcgcgacag
    56041 gcgggacagg aaccagttcc agatgcgtca ttgcggcaac ctcaccacga ggtcgtcagg
    56101 ccacggaagt tcttcattca cccgcaccca cgctacgccc ggtaaaccgg ttttgacata
    56161 ttccagatgc tgctggagta attccggtgg gatacgcgct ttgacgcgga acatcagttt
    56221 cagccgttca tcgctggttt cgacggtttt tggcgtgaac tgggcgacac tggcgacaaa
    56281 actgatggtt gcaggaatac gcagatctgg cgcggcatcg aggatcagcc gggcttcacc
    56341 gcccagtttc agcgtgcccg cctgttcggt tggcaggaag aaggtcatat agacgtcgct
    56401 gagatcgacc atattcagca cccgaccgcc tgccgccagc acttcccctg gctcggcaac
    56461 ccgatactgc acgcgtccgt cacgcggggc tttcagttca ctgtcatcga tatctgcggc
    56521 aatgcgccgt tcagtggctt gcgctgcttc gacccgagtt tgcgcctgaa tgatattggt
    56581 gcgtgctgct tctatagccg ctttagaagc cgaaacctgg gctttcgctg attccagcgc
    56641 agctcgggcg ctctcagcgg cggcgcggtc gtcatccagc tgttgcgcag aaatagcccc
    56701 tcgttgggcg agcgagcggg aacgcgtatg acgtttggca acagagtcca gttcggcctg
    56761 gcgttgattg accagcgact gtgcggcacg agtttcactt tgtcgttgct ccagcaaagc
    56821 ctgcgcggcg gcaacggcgc tttgtgcctc tttgatttgc gcgatggctt ccagtcgctg
    56881 ttcctgcaac acgcgagtat ccatcttcgc cagcacttcg ccttcgcgaa caaactggcc
    56941 ttctttcacc agaatggtgt cgatacgccc ggcagttttg ctggcgatat ccacttccgt
    57001 cgcttcaatg cgcccattgc tgacagcaaa gccttccggc acacccgccg ggcgcaacag
    57061 ccaccacgcc acgacggcag ccaccgccag tgccccgaca acccaccacg ccagatggcg
    57121 cttactctta tccataatcg acccgccata atccctgtaa gcaaacgacg cgtgttacaa
    57181 ctgccgccga acgacgttca gcggattctt ccatccggga taagaaggaa acgacaaata
    57241 cgaccagcgc gcagagcatt gagcgccaac ggggaaggct tagcgaaaac gggtagtaat
    57301 gttgtcatca tcagcattcc tggccgtaaa tgaaacacgc atactcataa gtgtgatcta
    57361 taaaactggc tgacaaaacg gtatccatcc tgttggatcg cgtttcacgc ctgttcagtt
    57421 gcaggaacag agcattcctt tttacgtttt atcaacagtc tcaaccagat agtaacatta
    57481 atattcatgt tgtctatggt tcagagctgt aacattgtgc gccttctaat caaaggcata
    57541 agtccatttc tgtttttttc atgctggtaa gacttccagg gagtggttat ctctggccct
    57601 gcaccagggt taatttggca attatgcatg ctgaacattc ttccagcatg tttactgtca
    57661 cgggcgttaa gacctaaaac tgtccccgtt tcacctatgc gttctctccc accatttccg
    57721 ttatgtgcaa caacttgatt tatacctaac atttgtgaac atgctaatag tgcttcaagt
    57781 ttaggtctgt aatctgtcga aacagaaaaa taacgattaa aggatcgaca agagatagcc
    57841 cggctagttt taaacttttt gccattaatt atagcatgat gctttttatt aataatttcc
    57901 gctaattcga ccggatcgag agcattggtc atttcatcaa tagagacagt tatcggtcct
    57961 ataatgtaac gttttcgctg atcatcaaaa gttatcccat gatgatttgc cattattttg
    58021 tatttggggt catagaaaca tacaggataa tcttttatcc cgtcgtaggt atcctcatca
    58081 agtggtttca tgcgcgtgaa gttttgcata tagttaccat tgcaattcgt ttcatgattt
    58141 ccagctaata catgaacatt tttattgata ctcattatgc gttttaacaa atcgatgata
    58201 aattgaacgc cacagatatt actaaatcga tcgccggttt ggtcgccaag aaacgtacat
    58261 ggcgtgactg cgtctttatt aataaccaaa taaggctgaa gactttctaa aacattatct
    58321 cttctgttca gtatactgat tattgtatta acattagtgt caagttgctc attaaagttc
    58381 tcccttagca atacgttgag aacttcatct tctatattaa gcaactggct aaggaaattt
    58441 tctccttcag ccatgattcc caaatgtcct gttgcaaaaa gcgcgtacaa aactgcaccg
    58501 actgagctat cggtatcgcc aaaataggtc ggcctggtac ttgtgggatc aacaggtgaa
    58561 caattggaga aataggttct gtcagcgaac tggggtttta taattgtttc gaatttaata
    58621 tcaaatacat tgtattgcga tatttcccag ttccagatgg gtttgtacga ctcagtaact
    58681 gcaacatcat tagcattatc atttactgtc atattattaa gtaaataaac aatgctgcac
    58741 caattaatat cggtattatc aagagggcaa ccgtgatttt tactattaat tgtcacaata
    58801 aactgataac agttattcct acgtagaata tgagcgcgca tgcccgatag catactatat
    58861 ttttctgaat gattcgtttg tatggtaaat gaaattttgt catcttgaat agattgttgg
    58921 aaacgttcta tttgccaatc atcaattttt tccacaactt caccgagcat tgtcctggca
    58981 atacttgata tatcatcacc tataccattt gcaatatacc caaacagttc tttagtgaag
    59041 ttggtaacat gctcttgcac catatcattt gttgcgattg tcgttgtact gccattagtg
    59101 cctgccacag ttccaatttt catcttttac ctcatattat tcaccgcgag gaatacatag
    59161 ttaacatttt atcttatgac atacacctca tattcgatgg gaaaataatt atattttgca
    59221 caaatcattc agccttaaag cataatatga aatttcttat ctcatagcag agaaagtccc
    59281 taaagattgt agaggcgtca tcagtacata cctctacaat cgattaagaa aacctatgaa
    59341 atactggcaa aattaaaaag agataccaca gcactcatga agttttattg aggttgaaaa
    59401 caactaagcc ctgggacacc ttttttatca ccacgttcgt taggtgctat cttccaagaa
    59461 tttagcccca tggtataatt atcaattttt tgatgctggt ctacccctaa tccataatgg
    59521 ccaaatttct gtttagtttt tttgagcgtc tgtccacatt tgaagatatc ttcaggtttt
    59581 ggtctgaaag cagtagtgtt attaaaataa ttttcatata caggtgtggg tgctatagca
    59641 ttgcttctaa ttagatggag aagatgatca tccatgtgtt gtttatgttt tttattaaat
    59701 atatttgcta attcctcagg atcaattggg tctttcatct gattaaatgg aacctgcaaa
    59761 gcaccgagac aataacattg actttcttca tttcttatta ttccgtgatg actggttaaa
    59821 actttagttt ctgagttata attacaaaca tcgagcgttt taattaagtt atacgtatca
    59881 ccggcagaga gtttataatt ttctagtcgt gcagtatagt taccattaaa attgatttca
    59941 tgatttccag ccagaacgat gacattttta ttaatacggc tatctttatt accctccata
    60001 tttcgcatag aatttagcaa agtaaggata tatttatcac caaaaatagt actaaatcta
    60061 tcgcctgtat gatccccaag ggggatacaa ggggttatcg catcgtcctt taaaaaaaca
    60121 tgttgatgca actcattcaa tatcatgtct ctcgtattta ataaattgta gagcaattta
    60181 ttatttttat gtatgtatgc agatgaagcc atgtcttcgt gttttaataa ttcacataat
    60241 aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatga ataaagtaca
    60301 gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg tgggttaatc
    60361 acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata gctatcaaac
    60421 ttatccttag gaaacgtctc tttagagaca tcccaacgcc agtcagcttt aaaattaccg
    60481 ctaagaagag taaagaaatt ttcaggtatg atagaaagcc cattaacttt ataaattatg
    60541 tctgatttat ctccggagaa attaataaaa taataaccat ccttacactc aatcttaact
    60601 gtaatgggag acgataattt tatcacatgg ttattgtctg gtataattgt aaagataaac
    60661 gtgttagtag tattatcggc aaaactatta tattcttgaa tatctataga tggctcacta
    60721 ttgtaaaacc atattttctc catctgaaga ctatgttcat ttaccctcca gtgaccatgt
    60781 tgcaggaaat agatgatatg catgccaaaa tcgttatcta tttgcgaatc attatccagt
    60841 ttatcaattt caactatctt tgaacgatgt gttttatcaa ccttgctatg gtgttgatga
    60901 ttattaactt tatctgaatg cttatcttta tggtttgaag ggatgtactt aaatatattg
    60961 tcaattgctt tcataaataa cccgcgatat ttttgacctg ttagtaaaaa ataaatgata
    61021 catgtctgaa agacatattt acgacgatat aaaacaataa tgcttatatg ttcgctgatt
    61081 ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca tccctggaag
    61141 acaattgtaa aaaaaacaac agaataagaa ttaatcttag gataaatatt tatttatcat
    61201 ggccttttga ccgctaaaaa atggcactcg gcacagtaaa ccccgctata gttatgctat
    61261 acatttgtct ttaatgatat ctcgtcgtta tattaacagg ctgaaattat cattaatgca
    61321 catttcaata ttagcaggga tacctgagga gtttaaagtg aacatatata tcgggtagct
    61381 tttcaaatta atccctttga ttataggctt aatttgcatc gcgttaggtg gctttgtgct
    61441 ggaaagttca gggcaacgtc acgggtcatg tgctgatttc tctggcggcc atatgcctgg
    61501 cattattcac taccgcattt attatcattt cgcagctcac gcgcggcgtt aatacgtttt
    61561 acaatacatt gttccccatt attggctatg cggggtcaat tatcaccatg atatggggtt
    61621 gggcactgtt agcaggtaat gatgtgatgg cagacgagtt tgttgccggc catgttattt
    61681 ttggcgttgg tatgattgcc gcatgtgtat cgacggtggc agcgtcgtcc ggtcactttc
    61741 tgctcattcc caaaaatgca gcggggagca agagcgacgg aacaccggta caggcttatt
    61801 cttcattaat cggtaattgc ctcattgccg ttcccgtttt actcaccctg ctcggtttca
    61861 tttggtctat tacgctgtta catagtgccg acataactcc gcattatgtc gccttgggtt
    61921 aaccgcaatc tgtgcctgtc taattggcct agtggccaca atcgtccatc aaacacgtaa
    61981 tacgttctca tccaaagagc actggctgtg gtgttattgg gttatttttc tcggctcagt
    62041 cacggtgctg caggggatat acgtcttagt cagttccgat gcaagcgccc gactggctcc
    62101 cggcattatt ctcatttgcc tcggaatgat ctgttacagc atattctcaa aagtctggct
    62161 actggcactg gtctggagac gtacctgttc gttagccaac agaataccga tgataccggt
    62221 atttacctgt ctgttttgct tgtttttgtc atcattcctc gcggaaatcg cacaaactga
    62281 gctggactac tttattccat ctcgggttct ggttggatta agcgcagtat gttttacatt
    62341 gttctctatt gtttcaatat tagaggccgg ttctgcgaaa aaaaattaaa atttacataa
    62401 tcccagcgtt attcatatct taacgctggt tttttacgaa cttagtgaaa taatgtagaa
    62461 tataaaatta taattttata ttggagcaca tgtgttaatg atcaaccccg ggatactaaa
    62521 aatatagaca ttattaaact tgcttagcct cgcattgcct ttacctgtta aagatgcatc
    62581 gagaaaattg agtctgaagt tctcatcctt tatctgattt tccagtcccg aaactttggc
    62641 cttagcatta cctgagatcc gtccgttact atctattttg taggtaaaaa aatacgaatg
    62701 ggaaaatttc ctcggtgtat cttcatcagt taagccaaca atcgtaaaac taccgctacc
    62761 atcttttttt aaaatgactg aaaggttagc agagtagtat aaagaatgat ctgttttagc
    62821 atttcttgtt aacgtcgacg tacattcaag agacgttgtg ctaccatcac gagccttcca
    62881 gagagcaaaa aaacacacct gtagcaaaaa tacattttct tattagtcat ataatagcac
    62941 ctctgaacaa attgcataag tttcgccatt agaggatttt atacatcttg ataaataccg
    63001 ctgcccttcc ccaagcaaat aatgcatact atttacatca tatataaaat ttgcacctgg
    63061 cacacagggt aaatgctttt tcgaaatttc agcggcctct atttgagatt tagcgaggcc
    63121 aatctcgtta agattcgctg aaagatataa atcacaggtg tcaatcttac ctgatttaac
    63181 caatggataa tccgttatac tgtaggtatc tttaaaagcc acggcaaaca tttcaataat
    63241 aacaattact accacaagcg aaatcaaaat gcgaaaaaga ggaatactcc ttttgcgctc
    63301 tactttttca acaacatctt cttgtaaatg agaatcatca taagattgtt tacttattgt
    63361 tatgttttca ctgatgtaaa agcctacttt gggtatcgta agaatcacat tttcttcaac
    63421 accaatattt ttaaaacttt tgcgtaacag ggatatatat tggttcaatg tgttgtttga
    63481 cagatcaaat gaaaatctct ccaaaacgct atcaatgatt gagttcctgt caaccgctcc
    63541 accattactt tccagtaaca ctgccaaaat acaccccgcc gtagctggca aatttatctc
    63601 atcctgggta tttacattcg agagcgagca tttgttcgga tcaaaaataa tcgcgtcatt
    63661 gataatgaaa aaacatctcc gctccatgca ggcaacaaca caaaataaga ttttgtcatt
    63721 ttagcctatt cctggtggaa ctgacacaag aaactaaaat acaaatataa ccctgaaaaa
    63781 gtcatataaa acttatatcc acagaataaa aaaacaaaac atgtcagttt tatcaatata
    63841 aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca
    63901 tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta
    63961 cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat
    64021 gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag
    64081 tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc
    64141 tcacaaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta
    64201 aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt
    64261 atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat
    64321 ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt
    64381 tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa
    64441 atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca
    64501 gatatactct ctcttcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt
    64561 aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt
    64621 gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta
    64681 attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt ctatgtcttt
    64741 ttttataagt taaaaaaggc gggatatcaa atcccgccat gaaattaact aatcatctgc
    64801 attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc tcaacaacct
    64861 gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt ttcagcatca
    64921 tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc tcaagcgttg
    64981 tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact gcatccgctg
    65041 aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg tggctctgcc
    65101 catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac gtaccgctat
    65161 ataccccttt cactggttga atggtattat taacaacgat aggcttggtc aaagtaagat
    65221 tagctgacgc ttcagtaccc tggcttacta aagttcccgc ctgagaagca aaagctgtcc
    65281 ccgccagcat gattgctaac aatgttttct tcattatatt ttctccatga ttttcattta
    65341 cactgcaaaa tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat gcttccacca
    65401 aataatggaa acatcttcac aaaaataaat tatgagtttc ttaatttaga aactataaac
    65461 atgtcgccgc tatctggtat aaccctgatg cacttttctt cgcaggaagt tggtattttg
    65521 cgatacagga ttgatcttta gaataacccc atttcacatt aaactcgcct tccgcactca
    65581 agccagaaac ataagcaata ccatcttcac ctacaatgcc ggccatattt tcagtgtctt
    65641 tgctataact aatcacagcg ccgaagggga cgggtttacc ctctttctgt tttaaggtta
    65701 ctaatccttt atccccgctc ttcactgcca atgtcgcagg cacaacggct ccagctgtcg
    65761 gtacaaccaa ttgactagtt tgtggtaagt cgtaattctt acctatcgtt ttttcatcca
    65821 gaataacatc gtctcttcgg tatggctgca ctggcagtat tgcataccca cgaaaatcag
    65881 tactcagtcc cggccagcca ttaacgttaa cccccgttgc tccgggtgcc tctataaggg
    65941 ctaatgtccc atccacattt tgaccaaatg taagtccatc ccttgtaata agtatcccac
    66001 cgcttaaatt tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg ctactaaact
    66061 gaccatatga tccatttttg ccaacttgca caatgttcat cgtgctcttc ggttgtgact
    66121 gatgagcgtt agttatattt gtcgctacat cccaccatac accaccacta ttctgacctg
    66181 ataatgaaac ctgatagtta ttaccctgct ctcccgaacg actcatctgt gaagacgcat
    66241 aaatttgatt ttcgtcaccc agcgataatg gcactgaaat acccacgctt aatttctgat
    66301 ctactttcca tttatgcgat gtcagcaagt cgttttttgc ccagttaatg gagaatgtta
    66361 tgcctctcca taccgtacta taactcaggc tggtagaccg cgtgtttcct gtgccccggg
    66421 aagaattata cctcgtggta ttgaagttca aattcccgat attaccaagg ggctgggata
    66481 aggatatcgt agag
//
LOCUS       DAJTYF010000008        65699 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTYF010000008 DAJTYF010000000
VERSION     DAJTYF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.008"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="OO590_000611"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="OO590_000611"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6636094.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OO590_000612"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OO590_000612"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6636095.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OO590_000613"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OO590_000613"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6636096.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OO590_000614"
     CDS             complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OO590_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6636097.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OO590_000615"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OO590_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6636098.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OO590_000616"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OO590_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6636099.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="OO590_000617"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="OO590_000617"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6636100.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7612..10146
                     /gene="mrcB"
                     /locus_tag="OO590_000618"
     CDS             7612..10146
                     /gene="mrcB"
                     /locus_tag="OO590_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6636101.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLAAIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10366..12609
                     /gene="fhuA"
                     /locus_tag="OO590_000619"
     CDS             10366..12609
                     /gene="fhuA"
                     /locus_tag="OO590_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6636102.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FGENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12660..13457
                     /gene="fhuC"
                     /locus_tag="OO590_000620"
     CDS             12660..13457
                     /gene="fhuC"
                     /locus_tag="OO590_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6636103.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13457..14347
                     /gene="fhuD"
                     /locus_tag="OO590_000621"
     CDS             13457..14347
                     /gene="fhuD"
                     /locus_tag="OO590_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6636104.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14344..16326
                     /gene="fhuB"
                     /locus_tag="OO590_000622"
     CDS             14344..16326
                     /gene="fhuB"
                     /locus_tag="OO590_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6636105.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16361..17641)
                     /gene="hemL"
                     /locus_tag="OO590_000623"
     CDS             complement(16361..17641)
                     /gene="hemL"
                     /locus_tag="OO590_000623"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6636106.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLMTEL
                     VPTIDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17866..19287
                     /gene="clcA"
                     /locus_tag="OO590_000624"
     CDS             17866..19287
                     /gene="clcA"
                     /locus_tag="OO590_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6636107.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGVLVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19311..19376
                     /locus_tag="OO590_000625"
     CDS             19311..19376
                     /locus_tag="OO590_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636108.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19369..19713
                     /gene="erpA"
                     /locus_tag="OO590_000626"
     CDS             19369..19713
                     /gene="erpA"
                     /locus_tag="OO590_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6636109.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19760..20383)
                     /locus_tag="OO590_000627"
     CDS             complement(19760..20383)
                     /locus_tag="OO590_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6636110.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20421..21221)
                     /gene="btuF"
                     /locus_tag="OO590_000628"
     CDS             complement(20421..21221)
                     /gene="btuF"
                     /locus_tag="OO590_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6636111.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21214..21912)
                     /gene="mtnN"
                     /locus_tag="OO590_000629"
     CDS             complement(21214..21912)
                     /gene="mtnN"
                     /locus_tag="OO590_000629"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6636112.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21996..23513
                     /gene="dgt"
                     /locus_tag="OO590_000630"
     CDS             21996..23513
                     /gene="dgt"
                     /locus_tag="OO590_000630"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6636113.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23643..25067
                     /gene="degP"
                     /locus_tag="OO590_000631"
     CDS             23643..25067
                     /gene="degP"
                     /locus_tag="OO590_000631"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6636114.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25222..26378
                     /gene="cdaR"
                     /locus_tag="OO590_000632"
                     /pseudo
     CDS             25222..26378
                     /gene="cdaR"
                     /locus_tag="OO590_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26467..26853)
                     /locus_tag="OO590_000633"
     CDS             complement(26467..26853)
                     /locus_tag="OO590_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6636115.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27167..27991)
                     /gene="dapD"
                     /locus_tag="OO590_000634"
     CDS             complement(27167..27991)
                     /gene="dapD"
                     /locus_tag="OO590_000634"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6636116.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28022..30694)
                     /gene="glnD"
                     /locus_tag="OO590_000635"
     CDS             complement(28022..30694)
                     /gene="glnD"
                     /locus_tag="OO590_000635"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6636117.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30756..31550)
                     /gene="map"
                     /locus_tag="OO590_000636"
     CDS             complement(30756..31550)
                     /gene="map"
                     /locus_tag="OO590_000636"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6636118.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31918..32643
                     /gene="rpsB"
                     /locus_tag="OO590_000637"
     CDS             31918..32643
                     /gene="rpsB"
                     /locus_tag="OO590_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6636119.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32901..33752
                     /gene="tsf"
                     /locus_tag="OO590_000638"
     CDS             32901..33752
                     /gene="tsf"
                     /locus_tag="OO590_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6636120.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33899..34624
                     /gene="pyrH"
                     /locus_tag="OO590_000639"
     CDS             33899..34624
                     /gene="pyrH"
                     /locus_tag="OO590_000639"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6636121.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34916..35473
                     /gene="frr"
                     /locus_tag="OO590_000640"
     CDS             34916..35473
                     /gene="frr"
                     /locus_tag="OO590_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6636122.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35565..36761
                     /gene="ispC"
                     /locus_tag="OO590_000641"
     CDS             35565..36761
                     /gene="ispC"
                     /locus_tag="OO590_000641"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6636123.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36950..37708
                     /gene="ispU"
                     /locus_tag="OO590_000642"
                     /gene_synonym="uppS"
     CDS             36950..37708
                     /gene="ispU"
                     /locus_tag="OO590_000642"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6636124.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37721..38578
                     /gene="cdsA"
                     /locus_tag="OO590_000643"
     CDS             37721..38578
                     /gene="cdsA"
                     /locus_tag="OO590_000643"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6636125.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWCADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38590..39942
                     /gene="rseP"
                     /locus_tag="OO590_000644"
     CDS             38590..39942
                     /gene="rseP"
                     /locus_tag="OO590_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6636126.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39972..42404
                     /gene="bamA"
                     /locus_tag="OO590_000645"
     CDS             39972..42404
                     /gene="bamA"
                     /locus_tag="OO590_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6636127.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42526..43011
                     /gene="skp"
                     /locus_tag="OO590_000646"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42526..43011
                     /gene="skp"
                     /locus_tag="OO590_000646"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6636128.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43015..44040
                     /gene="lpxD"
                     /locus_tag="OO590_000647"
     CDS             43015..44040
                     /gene="lpxD"
                     /locus_tag="OO590_000647"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6636129.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44145..44600
                     /gene="fabZ"
                     /locus_tag="OO590_000648"
     CDS             44145..44600
                     /gene="fabZ"
                     /locus_tag="OO590_000648"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6636130.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44604..45392
                     /gene="lpxA"
                     /locus_tag="OO590_000649"
     CDS             44604..45392
                     /gene="lpxA"
                     /locus_tag="OO590_000649"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6636131.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45392..46540
                     /gene="lpxB"
                     /locus_tag="OO590_000650"
     CDS             45392..46540
                     /gene="lpxB"
                     /locus_tag="OO590_000650"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6636132.1"
                     /translation="MTDQRPLTIALVAGEISGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46537..47133
                     /gene="rnhB"
                     /locus_tag="OO590_000651"
     CDS             46537..47133
                     /gene="rnhB"
                     /locus_tag="OO590_000651"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6636133.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47170..50652
                     /gene="dnaE"
                     /locus_tag="OO590_000652"
     CDS             47170..50652
                     /gene="dnaE"
                     /locus_tag="OO590_000652"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6636134.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50665..51624
                     /gene="accA"
                     /locus_tag="OO590_000653"
     CDS             50665..51624
                     /gene="accA"
                     /locus_tag="OO590_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6636135.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51723..53864
                     /gene="ldcC"
                     /locus_tag="OO590_000654"
     CDS             51723..53864
                     /gene="ldcC"
                     /locus_tag="OO590_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6636136.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53921..54310
                     /locus_tag="OO590_000655"
     CDS             53921..54310
                     /locus_tag="OO590_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6636137.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54375..55673
                     /gene="tilS"
                     /locus_tag="OO590_000656"
     CDS             54375..55673
                     /gene="tilS"
                     /locus_tag="OO590_000656"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6636138.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55722..55982)
                     /gene="rof"
                     /locus_tag="OO590_000657"
     CDS             complement(55722..55982)
                     /gene="rof"
                     /locus_tag="OO590_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6636139.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55969..56169)
                     /locus_tag="OO590_000658"
     CDS             complement(55969..56169)
                     /locus_tag="OO590_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6636140.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56335..56868
                     /locus_tag="OO590_000659"
     CDS             56335..56868
                     /locus_tag="OO590_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6636141.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAW"
     gene            56877..57299
                     /gene="arfB"
                     /locus_tag="OO590_000660"
     CDS             56877..57299
                     /gene="arfB"
                     /locus_tag="OO590_000660"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6636142.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57313..58023
                     /gene="nlpE"
                     /locus_tag="OO590_000661"
                     /gene_synonym="cutF"
     CDS             57313..58023
                     /gene="nlpE"
                     /locus_tag="OO590_000661"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6636143.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58178..59002)
                     /locus_tag="OO590_000662"
     CDS             complement(58178..59002)
                     /locus_tag="OO590_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6636144.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRNIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59055..60773)
                     /gene="proS"
                     /locus_tag="OO590_000663"
     CDS             complement(59055..60773)
                     /gene="proS"
                     /locus_tag="OO590_000663"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6636145.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60884..61591)
                     /gene="tsaA"
                     /locus_tag="OO590_000664"
     CDS             complement(60884..61591)
                     /gene="tsaA"
                     /locus_tag="OO590_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6636146.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61588..61992)
                     /gene="rcsF"
                     /locus_tag="OO590_000665"
     CDS             complement(61588..61992)
                     /gene="rcsF"
                     /locus_tag="OO590_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6636147.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62110..62925)
                     /gene="metQ"
                     /locus_tag="OO590_000666"
     CDS             complement(62110..62925)
                     /gene="metQ"
                     /locus_tag="OO590_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6636148.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62965..63618)
                     /gene="metI"
                     /locus_tag="OO590_000667"
     CDS             complement(62965..63618)
                     /gene="metI"
                     /locus_tag="OO590_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6636149.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63611..64642)
                     /gene="metN"
                     /locus_tag="OO590_000668"
     CDS             complement(63611..64642)
                     /gene="metN"
                     /locus_tag="OO590_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6636150.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64830..65405
                     /gene="gmhB"
                     /locus_tag="OO590_000669"
     CDS             64830..65405
                     /gene="gmhB"
                     /locus_tag="OO590_000669"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6636151.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15538 a        16270 c        18099 g        15792 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga ccctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacatga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tattcgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaacctgg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcgggtt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cgctgtatgg cgcgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccga acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaacccta accctctccc
     7501 tcaagggaga ggggaccgat cgagcacaaa ttttgagaga tatcttcttc tgtcttgtaa
     7561 cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag catggccggg
     7621 aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta
     7681 agccgtcgtc gttacgaaga tgacgatgat tacgacgatt atgatgacta tgaggatgaa
     7741 gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc
     7801 ggctggctat ggctactgct aaaactggct atcgtttttg ccgtgctgat cgccatttac
     7861 ggcgtttatc tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg gcaactgccc
     7921 gcggcagttt atggccgaat ggtcaacctt gaaccagata tgaccatcag caagaacgaa
     7981 atggtgaagc tgctggaggc aacacagtat cgtcaggtgt cgaaaatgac ccgtcctggc
     8041 gaatttaccg tgcaggccaa cagcattgag atgatccgcc gtccgtttga tttcccggac
     8101 agtaaagaag ggcaggtgcg tgcgcgtctg acctttgatg gtgatcatct ggcggccatc
     8161 gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg tctgatcacc
     8221 atgatctcct cgccaaacgg tgagcagcgt ttgtttgtgc cgcgcagtgg tttccctgat
     8281 ttgctggtgg atactttgct ggcgacagaa gaccgtcatt tttacgagca tgatggaatc
     8341 agtctctact ccattgggcg tgcggtgctg gcaaacctga ccgcaggtcg cacggtgcag
     8401 ggtgcgagta cgctgacgca acagctggtg aaaaacctgt tcctctccag cgagcgttct
     8461 tactggcgta aagcgaacga agcttacatg gcgctgatca tggacgcgcg ttacagcaaa
     8521 gaccgtattc ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa
     8581 atccgcggct tcccgctggc aagcttgtat tactttggtc gcccggtaga agagctaagc
     8641 ctcgaccagc aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta caacccgtgg
     8701 cgtaacccga aactggcgct ggagcgacgt aatctggtgc tgcgtctgct gcaacagcaa
     8761 cagattattg atcaagaact ctatgacatg ttgagtgccc gtccgctggg ggttcagccg
     8821 cgtggtgggg tgatctctcc tcagccagcc tttatgcagt tggtgcgtca ggagttgcag
     8881 gcaaaactgg gcgataaggt aaaagatctc tccggcgtga agatcttcac tacctttgac
     8941 tcggtggccc aggacgcggc agaaaaagcc gccgtggaag gcattccggc actgaagaaa
     9001 cagcgtaagt tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt tagtggtgaa
     9061 gttcgtgcga tggtcggagg ttctgagccg cagtttgcgg gctacaaccg tgcgatgcag
     9121 gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt atctgacggc tttaagccag
     9181 ccgaaaatct atcgcctgaa cacgtggatt gccgatgcgc caatcgcgct gcgtcagccg
     9241 aatggccagg tctggtcacc gcagaacgat gaccgtcgtt atagcgaaag cggcagagtg
     9301 atgctggtgg atgcgttgac acgttcgatg aacgtgccga cggtaaatct ggggatggcg
     9361 ctggggctgc ctgcggttac ggagacctgg atcaaactgg gcgtaccgaa agatcagttg
     9421 catccggttc cggcaatgct gctgggggcg ttgaacttaa cgccaatcga agtggcgcag
     9481 gcattccaga ccatcgccag cggtggtaac cgtgcaccgc tttctgcact gcgttcggta
     9541 atcgcggaag atggtaaggt gctgtatcag agcttcccgc aggcagaacg cgctgttccg
     9601 gcgcaggcgg cgtatctgac actatggacc atgcagcagg tggtacaacg tggtacgggt
     9661 cgtcagcttg gggcgaaata cccgaacctg catctggcag ggaaaacagg gactaccaac
     9721 aataacgtag atacctggtt tgcaggcatt gacggcagca cggtgaccat cacctgggta
     9781 ggccgtgata acaaccagcc gaccaaactg tatggagcca gcggggcaat gtcgatttat
     9841 cagcgttatc tggctaacca gacgccaacg ccgctgaatc ttgttccgcc agaagatatt
     9901 gcagatatgg gcgtggacta cgacggcaac tttgtttgca gcggtggcat gcgtgtcttg
     9961 ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca gcagcagccg
    10021 tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac agccgcaaca gcaacctgct
    10081 cagcaagagc agaaagacag cgacggtgta gccggttgga tcaaggatat gtttggtagt
    10141 aattaacatc taagcgtgaa ataccggatg gcgagttgcc atccggtaaa ataacatccc
    10201 atctaagata ttaacccttt cttttcatct ggttgtttat taacccttca ggaacgctca
    10261 gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat tatcttatct ttataataat
    10321 cattctcgtt tacgttatca ttcactttac atcagagata taccaatggc gcgttccaaa
    10381 actgctcagc caaaacactc actgcgtaaa atcgcagttg tagtagccac agcggttagc
    10441 ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag aagacactat caccgttacc
    10501 gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg caactattgc ggcgcggcag
    10561 tcagctaccg gcactaaaac cgatacgccg attcaaaaag tgccacagtc tatttctgtt
    10621 gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg taaaagaagc gcttagctac
    10681 acgccgggtg tctctgttgg tacgcgtggc gcatccaaca cctatgacca cctgatcatt
    10741 cgcggttttg cggcagaagg ccaaagccaa aataactatc tgaatggcct gaagttgcag
    10801 ggcaacttct ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc tgaaattatg
    10861 cgtggcccgg tttccgtgct ttacggtaaa agcagtcctg gcggtctgtt gaatatggtc
    10921 agcaagcgtc cgaccaccga accgctgaaa gaagttcagt ttaaagccgg tactgacagc
    10981 ctgttccaga ctggttttga ctttagcgat gcgttggatg atgacggtgt ttactcttat
    11041 cgcctgaccg gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga agagcagcgt
    11101 tatgctattg caccggcgtt cacctggcgt ccggatgata aaaccaattt caccttcctt
    11161 tcttacttcc agaacgagcc ggaaaccggt tattacggct ggttgccgaa agagggaacc
    11221 gttgagccgc tgccgaacgg taagcgtctg ccgacagact ttaatgaagg ggcgaagaac
    11281 aacacctatt ctcgtaatga gaagatggtc ggctacagct tcgatcacga atttaacgac
    11341 acctttactg tgcgtcagaa cctgcgcttt ggtgaaaaca aaacctcgca aaacagcgtt
    11401 tatggttacg gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg tgcggcatta
    11461 gcgccagcgg ataaaggcca ttatctggca cgtaaatacg tcgttgatga tgagaagctg
    11521 caaaacttct ccgttgatac ccagttgcag agcaagtttg ccactggcga tatcgaccac
    11581 accctgctga ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc ctggtttggt
    11641 tacgacgact ctgtgccact gctcaatctg tacaatccgg tgaatcccga tttcgacttc
    11701 aatgccaaag atccggcaaa ctccggccct taccgcattc tgaataaaca gaaacaaacg
    11761 ggcgtttatg ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct aggcggtcgt
    11821 tatgactggg cagatcaaga atctcttaac cgcgttgctg ggacgaccga taaacgtgat
    11881 gacaaacagt ttacctggcg tggtggtgtt aactacctgt ttgataatgg tgtaacacct
    11941 tacttcagct atagcgaatc gtttgaacct tcttcgcaag ttgggaagga tggtaatatt
    12001 ttcgcaccgt ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc ggaagatcgt
    12061 ccgattgtag ttactggtgc cgtgtataat ctcactaaaa ccaacaacct gatggcggac
    12121 cctgagggtt ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg cgtagaaatc
    12181 gaagcgaaag cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac ttacaccgat
    12241 gcggaataca ccaccgatac tacctataaa ggcaatacgc ctgcacaggt gccaaaacac
    12301 atggcttcgc tgtgggctga ctataccttc tttgacggtc cgctttcagg tttgacgctg
    12361 ggcaccggtg gtcgttatac tggctccagc tatggtgatc cggctaactc ctttaaagtg
    12421 ggaagttata cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt cggcatggcg
    12481 ggctccaacg tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt cgccagctgc
    12541 tttaacactt atggctgctt ctggggcgca gaacgtcagg tcgttgcaac cgcaaccttt
    12601 cgtttctaat ttctcttttg gggcacggat ttccgtgccc atttcacaag ttggctgtta
    12661 tgcaggaata cacgaatcat tccgatacca cttttgcact gcgtaatatc tcctttcgtg
    12721 tgcccgggcg cacgcttttg catccgctgt cgttaacctt tcctgccggg aaagtgaccg
    12781 gtctgattgg tcacaacggt tctggtaaat ccactctgct caaaatgctt ggtcgtcatc
    12841 agcctccgtc agaaggggag attcttcttg atgcccagcc gctggaaagc tggagcagca
    12901 aagcgtttgc ccgcaaagtg gcttatttgc cgcagcagct tcctccggca gaagggatga
    12961 ccgtgcgtga actggtggcg attggtcgtt acccgtggca tggcgcgctg gggcgctttg
    13021 gtgcggcaga tcgcgaaaag gtcgaggaag ctatctcgct ggttggctta aaaccgctgg
    13081 cgcatcggct ggtcgatagt ctctctggcg gcgaacgtca gcgggcgtgg atcgccatgc
    13141 tggtggcgca ggatagccgt tgtctgttgc tcgacgaacc gacttcggcg ctggatatcg
    13201 cccaccaggt tgatgtgctg gcgctggtgc accgtttaag tcaggaacgt ggcctgacgg
    13261 tcattgccgt gttgcacgat atcaatatgg cggcacgcta ctgtgattat ctggtcgccc
    13321 tgcgcagtgg tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc ggcgaaaccc
    13381 tcgaaatgat ttatggcatc ccgatgggta ttttgccgca tccggcgggt gctgcacctg
    13441 tgagttttgt ttattgatga gcggcttacc tcttatttcg cgccgtcgac tgttaacggc
    13501 gatggcgctt tctccgttgt tatggcagat gaataccgcc cacgcggcgg ctattgaccc
    13561 caatcgtatt gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc tcggcatcgt
    13621 gccttacggc gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac caccattgcc
    13681 ggactcagtg attgacgtcg gtttgcgtac agaacctaac cttgaactgc tgaccgaaat
    13741 gaaaccgtcg tttatggtct ggtcggcagg atatggccct tcatcagaaa tgctggcacg
    13801 tattgcgccg ggtcgcggat ttaacttcag tgacggcaaa catccgttgg cgatggcgcg
    13861 taaatcgctg acggaaatgg cagatttact taacctgcaa agcgcagcgg aaacgcattt
    13921 agcgcaatac gaagacttta tccgcagcat gaaaccccgc tttgtgaagc gtggtgcacg
    13981 cccgttactg ctgacgacgc ttatcgatcc gcgccatatg ctggtcttcg gtccaaacag
    14041 cttgttccag gaaattctcg atgagtacgg catcccaaat gcctggcaag gggaaaccaa
    14101 cttctggggc agtaccgccg tcagtatcga tcgtctggca gcgtataaag acgttgatgt
    14161 gctctgtttt gatcacgaca acagcaaaga catggatgcg ctaatggcaa ccccgctgtg
    14221 gcaggccatg ccgtttgtcc gtacagggcg ctttcagcgc gtacccgcag tctggtttta
    14281 tggtgcaacg ctttcggcaa tgcactttgt gcgtgttctg gataacgcca tcggaggtaa
    14341 agcgtgagta aacgaattgc gcttttcccg gcgttattgc tggcgctgtt agtgattgtc
    14401 gctacggcgc tcacctggat gaacttctca caggcgctgc cgcgcagtca gtgggcgcag
    14461 gccgcctggt cgccggatat tgacgtcatc gagcagatga tttttcacta cagcttgttg
    14521 ccgcgtctgg cgatttcgct gctggtgggc gcgggcctgg ggctggtggg cgtgctgttt
    14581 cagcaagtac tgcgtaaccc gctggcggaa ccgacgacgt tgggcgttgc aacaggcgcg
    14641 caactgggga ttaccgtcac cacgctctgg gcgatcccta gcgcgatggc gagccagttt
    14701 gctgcgctgg caggggcttg cgttgttggc ttaattgtct ttggcgtcgc gtgggggaaa
    14761 cggctgtcgc cggtaacgct gattctcgcg gggttggtag tgagccttta ttgcggcgca
    14821 atcaatcagt tactggttat cttccatcat gaccagttgc aaagcatgtt tctgtggagc
    14881 actggaacgc tgacgcaaac cgactggggc ggtgttgagc gtttatggcc gcagttgctg
    14941 ggcggtgtga tgctgacgtt gctgctactt cgtccgttaa ccctgatggg gcttgatgat
    15001 ggcgtggcgc gcaatctcgg gctggccttg tcgcttgcgc gtctggcggc gctgtcgctg
    15061 gcgattgtca tcagtgcgct gctggtgaac gctgtgggga ttatcggctt tatcgggttg
    15121 ttcgcgccgc tgctggcaaa aatgctgggg gcgcggcgtc tgctgccacg actgatgctg
    15181 gcgtcgttga ttggtgcgct gatcctctgg ctttccgatc aaatcatcct ctggctgact
    15241 cgcgtgtgga tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc gccgctgcta
    15301 ctgtggctgc tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt taacgatcgt
    15361 gtcgcgactg aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt gctgttgttg
    15421 atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc acggctggac gtgggcgagc
    15481 ggggcgttgc tcgaggattt aatgccctgg cgctggccgc gaattatggc ggcgctgttt
    15541 gcgggcgtca tgctggcggt agcgggctgt attattcagc gactgaccgg caacccgatg
    15601 gcaagcccgg aagtgctggg gattagctcc ggcgcggcgt ttggtgtggt gttaatgctg
    15661 tttctggtac cgggtaatgc ctttggctgg ttgttacccg caggcagtct cggggcggcg
    15721 gtgatgctgt tgatcattat gatcgccgcc ggacgcggtg gattttcccc acaccgcatg
    15781 ttactggcgg ggatggcgtt aagtaccgcg ttcaccatgc ttttgatgat gttgcaggca
    15841 agtggtgacc cgcgaatggc gcaagtgctg acctggattt ccggttcgac ctacaacgcg
    15901 accgatgctc aggtctggcg caccggaatt gtgatggtga ttttgctggc gattactccg
    15961 ctgtgccgcc gctggctgac cattttaccg ctgggtggtg ataccgcgcg ggcggtaggg
    16021 atggcgctga cgccgacgcg aattgcgctg ctgctgttag cggcttgcct gacggcgacc
    16081 gcgacgatga ctattggacc gttgagtttt gttggtttaa tggcaccgca tattgcgcgg
    16141 atgatgggct ttcgacggac gatgccacat atcgtaattt cggcgctggt gggtggttta
    16201 ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt ttccattcca gatcccggcg
    16261 gggctgctgt cgacctttat cggtgcgcca tattttatct atttgctgag aaagcagagc
    16321 cgttaataga atgcctcgcc cggtgggcga ggcaaaatta tcacaacttc gcgaacaccc
    16381 gacgtgcagc atcgatggtg ttattgatat cttccatgct gtgcgccaca gacataaagc
    16441 ccgcttcaaa cgctgacggt gccaggtaaa caccttcatc cagcatcata tggaagaaac
    16501 gcttaaaacg ctcgacgtca caggccatca catcctgata gcacgtcacg gactcggcgt
    16561 cggtaaagaa gataccgaac ataccaccga cgtggttaac gaccagcgga attcccgctt
    16621 cttctgccgc ttccagcaga ccttctgcca gacgtgttgt cagctcatcc agcgtttcgt
    16681 gaatgcccgg ctgcgcgact tcattcagac aggcgaaacc cgctgccatc gcaatcgggt
    16741 taccggaaag cgtacccgcc tgatagaccg gacctgtagg ggccagcgca tccattacat
    16801 cacgacgacc accgaatgcg cctaccggca ttccaccgcc gatgattttt ccgaggcagg
    16861 ttaaatctgg cactacgccg aaataatcct gtgcgccagc tagcgctacg cggaaaccgg
    16921 tcatcacttc atcgatgatc agcaacgcgc caaattcgtc gcacagcgca cgcagacctg
    16981 gcaggaactc tggcagcggc ggaacacagt tcatattgcc tgccaccggc tcgacgataa
    17041 tacaggcaat ctcttgcggg tattgctcaa acgcggcgcg tacagaggcc agatcgttat
    17101 aagtacaggt taaggtatgt ttggcgaaat ctgccggaac gcccggcgag tttggctggc
    17161 ctaacgtgag tgcgccagaa ccggctttca ccagcaggca gtcagcgtga ccgtggtaac
    17221 aaccttcaaa tttaatgatt ttgtcgcgac cggtaaaacc acgggccaag cggatggcgc
    17281 tcatcgtcgc ctcggtgccg gagttcacca tgcgcaccat atctatggtc gggaccagtt
    17341 cggtcatcag ttgcgccatt ttcacttcca tttcggtcgg tgcaccaaag cttaaaccac
    17401 gctcggcggc ttcaatcacg gcattgcgga tcgccggatg gttatggccc agcaccatcg
    17461 gcccccagga accgacataa tcgatatagg ctttgccatc aacatcgtac agataagcgc
    17521 cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg
    17581 agttcacacc gccagggatc agctcgcgcg ctgcgctgta aagattttca gacttactca
    17641 tggagggttc ctgattcgta gaaaaagtga atggctgcta ttctatgtta ttcataacaa
    17701 gttaaatact cgtcaaacat caggctgctt gtactggtgc aatcctgatt tcttagagta
    17761 taaaagtttt gtgcatttga aacattacgc tttgcaaagg attttcatgg aacgtgcgag
    17821 taaaatgccg tcatcttatt tgtatgacca ataagtgatc attggatgaa aactgatact
    17881 ccctctttag aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt
    17941 cttgagcgcg ataaaacccc gttagccatt ttgtttatgg cggcagtcgt cggcacgctt
    18001 atcgggctgg ctgcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg
    18061 ggagtgctgg tacatactgc tgataattat ccgctactgt taaccgtcgc ttttctctgt
    18121 tcggcggtgc tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc cgaagcaggg
    18181 ggatcgggga tcccggaaat tgaaggggcg ctggaagatc aacgtcctgt tcgctggtgg
    18241 cgtgtattgc cggtgaaatt ctttggcggg ctggggacac tcggcggagg catggtgcta
    18301 gggcgcgaag gaccaaccgt gcagatcggc ggcaatatcg gtcgcatggt gcttgatatt
    18361 ttccgcctga aaggtgatga agctcgccat acgctgctgg caaccggtgc tgctgcgggg
    18421 ctggctgcgg cctttaacgc gccgctggcg ggtattttgt ttattatcga agagatgcgt
    18481 ccgcagtttc gctatacgtt aatttcgatt aaagcggtat ttattggcgt cattatgtcg
    18541 accattatgt accggatttt taatcatgaa gttgcgttga ttgacgtcgg taaactttct
    18601 gatgcgccgc ttaatacgct gtggctttat ctgatcctcg gtattatttt tggcattttc
    18661 ggccctattt ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc
    18721 ggcaatatta ccaaatgggt actgatgggg ggggcgattg gcggtctgtg tggattgctg
    18781 gggtttgtgg caccagcaac gtcgggcggc ggttttaacc tgattcctat cgctaccgcg
    18841 gggaatttca gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat taccacctta
    18901 ctctgcttct cttccggcgc gccgggcggt atttttgccc cgatgctggc gctgggtact
    18961 gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt ttccgcaata tcacctggag
    19021 gcggggacgt ttgctattgc cggaatgggg gcattactgg cggcatctat tcgcgcgccg
    19081 ttaacgggga tcattctggt tctggagatg accgataact accagctcat tttgccaatg
    19141 attattaccg gtcttggcgc aacactatta gcgcaattta ccggcgggaa acctctatac
    19201 tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg agcaactggc gcgaagcaag
    19261 gccgcatcag ccagcgagaa tacttgaacg aaataccagg gtattagata atggcgatta
    19321 ttattgggtt agaatttgcc cgattgccga tgtcgtttgg agcaaaatat gagtgatgac
    19381 gtagcactgc cgctggagtt taccgacgca gcagccaaca aagttaaaag cctgatcgct
    19441 gacgaagata acccgaatct gaaattacgc gtgtatatca ccggtggcgg ttgcagcggc
    19501 ttccagtatg gtttcacctt tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa
    19561 cagggcgttg gcctggtggt tgatccgatg agcctgcaat atctggtcgg cggttccgtt
    19621 gactacaccg aaggactgga aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc
    19681 acctgcggtt gcggttcttc ctttagcatc taatctgttg ttcatgtgcc ggataaacta
    19741 tccggcacat cttctctctt caacgcccat tctcatccag cgcaaacgtc ggtagcttaa
    19801 gatgccaacg aatagccgcc agccgaatca atagcgtcac gaccatgccc atcatactgg
    19861 ctgtttccag tggtacggaa aatgtgtaat aagccgtagc gtggacaata ccgccgataa
    19921 tacaggcagt tgcgtagatt tctgtacgta aaatcatggg gatttcgcgg gccagtacat
    19981 cacgaatgat cccgccgcca acgccagtaa tgacgcccat acaaaccgcg attaacggac
    20041 cggcttccgc attaaaggct ttattcacgc caatgccgac aaacaccgcc agaccaacgg
    20101 cgtccagcac cggcaacatc cattttggta agcgtcttgg ctggcgcacc agcacgatgg
    20161 tcagcatgct ggtgaccatg gcaacgacca gatcggtggg atctttcacc caaaataccg
    20221 ggccgtgatc cagcgccatg tcgcgaattg ttccgtcgcc tactgcggta accacgccca
    20281 gtaccagaac accaaaaggg tccatacgca attttccggc taacaaaacg ccggagatgg
    20341 caaataccgc tgtgccgact atatccagcc aatagacgag cattgttcaa tccccactga
    20401 gcacctgaaa aggtcaggca ctaatctacc tgtgaaagcg cattacagag ctgttgtgct
    20461 gcgaggataa tacgtgggct tgcacgttca aaccagtcac tcgtgagagg aataacggga
    20521 attttgagct gttcacccca gtattgtttg attttaggaa tttggtccgg tccgcctgta
    20581 atgacaatcg cctgtggcga gcgtgctaac acctgttcgc ggctaacttg cggccaggga
    20641 acccggctgt ctttaaagat gttttctccg ccacaaactt cgagtacctg gttctgaatc
    20701 gactcttttc cactggtaaa tggcggatta atgccgaatt gcagaaaaac acgtttttta
    20761 ggtttatcag catattgcgc tttcaattgc acgtactgat ccagcaggga ttgcgcggct
    20821 tgttcggcct tgtctggttg cggactccag ggggccagtt gacgtaacgc attggcaatt
    20881 tgttcaatgc ttgtcgcatc gacccacatc acttttattc ccagcgaagc cagctggtca
    20941 acctgccgct cggcattacc tccacgccag gcaatcacca gatcgggttt cagcgcgaca
    21001 atgcgttcca gattcatccc ctgccaggtg gaaacctgct caatcttttg cgcttgtgga
    21061 ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa ggcaagttca
    21121 gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag tggcgcgaga
    21181 aaagacaggg cgaccagcgc cctgaacagt gatttagcca tgtgccagtt tctgcaccag
    21241 tgactcaacc atcaggctgg actgtttagc ggcaacagcc aggaactcat cgaagctaag
    21301 atgagactgt tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa
    21361 attgtggcag acatgggcga ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa
    21421 gttgtggcgg attttcgcca gaccaacaga accgttgatg aaagcgtcgc cgctaacaat
    21481 caggccacgt acagcgttaa gattcagttc ggcaatgcag gcctcagcgg cagcgatcag
    21541 tttatcgtca gctttaaagc ctgtcggaca gcctggtaac tgaccgtatt cataaccaaa
    21601 tgccgtgaca tccgcgtcgt gataacgcgc ttcgtccgag acaacgatat cgcccacttt
    21661 cagcgttggt gccaggccac cggcagaacc ggtgttaata atcacatctg gcttgcagtg
    21721 ttccaacagc agagtggcac ccagcgccgc agcgacttta ccgatgcccg atttcagaag
    21781 cgcaacctcg gttccattca gttggccggt atagatttcg caaccgccga gactgatagt
    21841 ttgacggttt tcgattttgt cacgcagcag cgtaacttct tcttccattg caccaatgat
    21901 gccgattttc atagatttac tcgcgataag cccgatttga aggcatagtt taccatgcgc
    21961 ttacggggaa gcgtatttct cacgcgggag aggacatggc acagattgat ttccgaaaaa
    22021 aaataaactg gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa catgagatcc
    22081 tgcggatctt cgagagcgat cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc
    22141 aacaaaagac ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc
    22201 actcgatgga agtccagcag gtgggacgct acatcgccaa agaaatttta agccgtctga
    22261 aagagcttaa attgctggaa gcatacggcc tggatgaact gaccggcccc tttgaaagca
    22321 ttgttgagat gtcatgcctg atgcacgata tcggcaatcc gccgtttggt cattttggcg
    22381 aagcagcgat aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc
    22441 ctctgactga cgatcgctgc agcgtggcgg cactacgttt acgggacggg gaagaaccgc
    22501 ttaacgagct gcggcgcaag attcgtcagg acttatgtca ttttgagggg aatgcacaag
    22561 gcattcgtct ggtgcataca ttgatgcgga tgaatctcac ttgggcacag gttggcggta
    22621 ttttaaaata tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca catcactatt
    22681 taatgaaaaa gccgggttat tatctttctg aagaagccta tattgcccgg ttgcgtaaag
    22741 aacttaattt ggcgctttac agtcgttttc cattaacgtg gattatggaa gctgccgacg
    22801 acatctccta ttgtgtggca gaccttgaag atgcggtaga gaaaagaata tttaccgttg
    22861 agcagcttta tcatcatttg cacgaagcgt ggggccagca tgagaaaggt tcgctctttt
    22921 cgctggtggt tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc cgcagtacgg
    22981 aagatcagtt ttttatgtat ttacgggtaa acaccctaaa caaactggta ccctacgcgg
    23041 cacaacgatt tattgataat ctgcctgcga ttttcgccgg aacgtttaat catgcattat
    23101 tggaagatgc cagcgaatgc agcgatcttc ttaagctata taaaaatgtc gctgtaaaac
    23161 atgtatttag ccatccggat gtcgagcagc ttgaattgca gggctatcgg gtcattagcg
    23221 gattattaga gatttatcgt cctttattaa gcctgtcgtt atcagacttt actgaactgg
    23281 tagaaaaaga acgggtgaaa cgtttcccta ttgagtcgcg cttattccac aaactctcga
    23341 cgcgccatcg gctggcctat gtcgaggctg tcagtaaatt accgtcagat tctcctgagt
    23401 ttccgctatg ggaatattat taccgttgcc gcctgctgca ggattatatc agcggtatga
    23461 ccgacctcta tgcgtgggat gaataccgac gtctgatggc cgtagaacaa taaccaggct
    23521 tttgtaaaga cgaacaataa atttttacct tttgcagaaa ctttagttcg gaacttcggg
    23581 ctataaaacg aatctgaaga acacagcaat tttgcgttat ctgttaatcg agactgaaat
    23641 acatgaaaaa aaccacatta gcactgagtg cactggctct gagtttaggt ttggcgttat
    23701 ctccgctctc tgcaacggcg gctgagactt cttcagcaac gacagcccag cagatgccaa
    23761 gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt cagcattaac gtagaaggta
    23821 gcacaaccgt taatacgccg cgtatgccgc gtaatttcca gcagttcttc ggtgatgatt
    23881 ctccgttctg ccaggaaggt tctccgttcc agagctctcc gttctgccag gggggccagg
    23941 gcggtaatgg cggcggccag caacagaaat tcatggcgct gggttccggc gtcatcattg
    24001 atgccgataa aggctatgtc gtcaccaaca accacgttgt tgataacgcg acggtgatta
    24061 aagtccagct gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa gatccgcgct
    24121 ctgatatcgc gctgatccaa atccagaacc cgaaaaaact gaccgcaatt aagatggcgg
    24181 attctgatgc actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg tttggtctgg
    24241 gcgagacggt aacttccggg attgtctctg cgctggggcg tagcggcctg aatgctgaaa
    24301 actacgaaaa cttcatccag accgatgcag cgatcaaccg tggtaactcc ggtggtgcgc
    24361 tggttaacct gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca ccggacggcg
    24421 gcaacatcgg tatcggtttt gctatcccga gtaacatggt gaaaaacctg acctcgcaga
    24481 tggtggaata cgggcaggtg aaacgcggtg agctgggtat tatggggact gagctgaatt
    24541 ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta agccaggttc
    24601 tgcctaattc ctcggctgca aaagcgggca ttaaagcggg tgatgtgatc acctcactga
    24661 acggtaagcc gatcagcagc tttgccgcac tgcgtgctca ggtgggtact atgccggtgg
    24721 gcagcaaact gaccctgggc ttactgcgcg acggtaagca ggttaacgtg aacctggaac
    24781 tgcagcagag cagccagaat caggttgatt ccagctccat cttcaacggc attgaaggcg
    24841 ctgagatgag caacaaaggt aaagatcagg gcgtggtagt gaacaacgtg aagacgggca
    24901 ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat tattggtgcg aaccagcagg
    24961 cagtgaaaaa catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct gtgctggcac
    25021 tgaacattca gcgcggcgac agcaccatct acctgttaat gcagtaatct ccctcaaccc
    25081 cttcctgaat acgggaaggg gttctcctta caatctgtga acttcgccac aactccatac
    25141 atcttcatca tcctttgggc atttgcacaa tgccgtacgt tacgtacttc cttatgctaa
    25201 gccgtgcata acggaggact tatggctggc tggcatcttg ataccaaaat ggcgcaggat
    25261 atcgtggcac gtaccatgcg catcatcgat accaatatca acgtaatgga tgcccgcggg
    25321 cgaattatcg gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg tgcattgctg
    25381 gtactttcac agggacgagt cgtcgatatc gatgacgcgg tagcacgtca tctgcacggt
    25441 gtgcggcagg ggattaatct accgttacgg ctggaaggtg aaattgtcgg cgtaattggc
    25501 ctgacaggtg aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat gacggctgaa
    25561 atgatgctgg aacagtcgcg gttgatgcac ttgttggcgc aggatagccg tttgcgggaa
    25621 gaactggtga tgaacctgat tcaggcagag gagaatactc ccgcacttct gaatgggcgc
    25681 aacggctggg gatcgatctc aatcaaccgc gagtggtggc tattgttgag gtcgacagcg
    25741 gtcagcttgg cgtggacagc gcaatggcgg agttacaaca actgcaaaac gcgctgacta
    25801 cgcccgagcg taataatctg gtggcgattg tctcgctaac cgaaatggtg gtgttgaaac
    25861 cggcgttgaa ctcttttggg cgctgggatg cagaagatca tcgtaagcga gttgaacaac
    25921 tgattacccg catgaaagag tacggccagc tgcgttttcg cgtttcactg ggcaactatt
    25981 ttaccggtcc tagcagtatt gcccgatcct atcgtacggc gaaaacgacg atggtggtgg
    26041 gtaaacagcg gatgccagaa agtcgctgct atttttatca ggatctgatg ttacctgtgt
    26101 tactcgacag tttgcgtggc gactggcagg ccaacgaact ggcgcgaccg ctggcgcggc
    26161 tgaaagcgat ggacaataac ggcttgctgc gacgaacgct ggcggcgtgg tttcgccaca
    26221 atgtgcaacc gctggcaacg tcaaaggcgt tgtttattca tcgtaatacc ctggagtatc
    26281 ggcttaatcg tatatcggaa ctgaccgggc ttgatttggg caattttgat gacaggttgc
    26341 tgctgtatgt ggcgttacaa ctggatgaag agcggtagat tatgcgttaa ggttgaggcg
    26401 gcgctggctc attcccctca gcccgaaggg gagaggggaa tgcccgagtt aaccgtcaat
    26461 tcgggattat ttacgcgtta gtttttccag atcggcttca atctcgctga tcttattggt
    26521 tacgactgac tccaggtgac gtaagtcgtc gaggatctta cgcttaagat caacttcgct
    26581 gcggtcacgc tgacagattt gatcaagctc atcaatgata taccgtagat tcgggctgat
    26641 ttcctggact tctttataac cctgacccac accatcagcg acgaccgttt tacgctgacg
    26701 cggatattta aacttaacgc tcttggcgaa aaactcgcct ttgtctttct ggaaatagat
    26761 tttcagaata tcgttgttgg cttcctgccg gaggctgtaa cgatcaattt cttcaggatt
    26821 ggtaataccc agacttttca gattgtcgta catagcgtta cctcaaaatg agtcagtaaa
    26881 ttgtgcttat tttagcattt ggcttggccc gcccggctcg actgtgatcg cagaaagcct
    26941 ttttcttcca gacttctatc atggcgtaaa aatcaaaaaa ttacctgctt tattgtggcg
    27001 atattccatg ggatggatta gcacatttat cttcgttttg cgaattacat gacagtccga
    27061 aacaagatga aaaagtattt aaaaaagggg gaggaagaat gagtaggacg aattattctg
    27121 agccagaaag catacgggca gcatcatgct gcccgtatat gtactcttaa tcgatggtac
    27181 gcagcagttc gttaatgccg actttgccgc gagttttcgc gtcaactttc ttaacgataa
    27241 ccgcacagta gaggctgtat ttgccatctt ttgacggcag attacctgaa acaaccacag
    27301 accccgccgg aacgcgaccg tagtggattt cgccggtttc acggtcgtaa atacgggtgc
    27361 tctgaccaat gtatacgccc atagagatga cggaaccttc ttcgacaatc accccttcaa
    27421 ccacttcaga gcgtgcgcca atgaagcagt tatcttcaat aatggtcggg ttagcctgca
    27481 gcggctccag cacgccgccg atgcccacgc caccggaaag gtggacgttt ttaccaatct
    27541 gcgcacaaga accgacggtc gcccaggtat caaccatggt gccttcatca acatatgcgc
    27601 cgatgttaac gtaagacggc atcagcacgg tgttacgggc aataaacgca ccctgacgta
    27661 ccgccgctgg tggcacaacg cggaagcctt ctttctggaa acgtgcttcg tcgtagtcgg
    27721 cgaatttcat cggcactttg tcgaagtagc ggctttctgc cccttcgatc acctgattat
    27781 cattaatacg gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc acccactgac
    27841 cgtcaatttt ttccgctaca cgcagtgcgc cggaatccag cagggcgatc acctgattta
    27901 ccgcttcgcg ggtaacggtg tctgcattgg ctggcgtgat ctcggcacgg cgttcaaaag
    27961 cggtttcaat aatgttctgt aactgctgca ttgttaaact cttttcatat cagtaaacac
    28021 atcacccttt atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact tcctgctgca
    28081 actcgttatt aagcgcacgc cggtcagcgg tggcaattat gaataaatct tctactcgct
    28141 cgccaatggt tgtaattcgg gcaccatgaa gcgaaattcc cagatcggca aaaattttcc
    28201 cgactcgcgc cagcagtcca ggttggtcga gggcgatcag ttcgaggaac gatttgcggt
    28261 cggtatgggt cggcaaaaac gttacttcgg tttcaacagt aaaatggcgt aatttggcgg
    28321 gttggcgacg gggctgcggt ggctgccagc tactttgcgt cagcacctgc tccagaccaa
    28381 accgaataac ctcatggcga tctgcggaca gcgggctgcc atcgggttcc agcacgataa
    28441 aggtatccat cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga acacttaaat
    28501 tgcggcggtc taattcggca cagacggcgg caaacagata ggggcggtcc gggctccaga
    28561 taaaaatctc ggtgcctcca cgcgtagcct gcgggctaag caataccagc ggtttgctta
    28621 aatcatgctg taataaatgg cgggcatgcc aggccagttg atttgggcta tggcggacaa
    28681 aatagttagc acgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg atgttatcca
    28741 tgcgcagtag tgccagtgcc tggagttggt gatggcgaac ccgttcgcgc atatccggcg
    28801 tgttttgcat tccgcgtcgt agctgctttt cggtggcaaa atagagttcg cgcaacagac
    28861 tttgcttcca gctattccac agcgtttcgt tggtggcgca aatgtcagcc actgtcaggc
    28921 ataccagata gcgcagacga ttttccgttt gaacttcttc ggcaaactgc ttgatgactt
    28981 cagggtcctg aatatcgcgg cgttgtgctg tcaccgacat cagcaagtgc tggcgaacca
    29041 gccaggcaac cagctgtgtt tcgcgtgagt tcagcccgtg gagttctgca aaatgcacta
    29101 catcctgagc accgagaatg gagtggtcgc cgccgcgtcc tttggcgata tcgtgaaaca
    29161 gcgcggcgat gaaaatcagc tcagttgacg gcaggcgcgg ccagacgtcc acacacaacg
    29221 gatggcgctg gcgcgtttct tcactggcaa aactctccag tttcagcatc acgcggatag
    29281 tatgttcatc caccgtgtag gcgtggaaca gatcaaactg catctgcccg acgatatgcg
    29341 accattgcgg catatacgcg ccgagcacgc tatggcgatg cattggcaat agcccgcgcc
    29401 gcaccgctcc ggggtgacgc agaatgctca aaaacagttt tcgtgcttgc ggaatattac
    29461 acagcggttg ttgcagatgg cgacgggcat ggcgtaactg gcgcagcgtg gtggagtaaa
    29521 taccggtgat cgcactgttg cgcaccatgg tgtagaacat acgcaagatg gcttccggct
    29581 ggcgcataaa tagtgtttca tcacgcaggt cgattagcgt accgcgtagc tgaaactcat
    29641 cgtcgagtgg acgtggtttt tcgtcggcgg gaagggcgag gatggcttca tcgaacagtt
    29701 gcagcagcat ctggttgagt tcactgacgc ggcgtgtaac gcggaagtaa tccttcatca
    29761 tccgctcgac cggttcgttg ccttcaccac tgtaattcag acgctgagcg acgctaagtt
    29821 ggcgatcgaa taacagacga ttatcgtaac ggctgacgac cagatgcagg gcaaagcgaa
    29881 tacgccacag tatatgcaga cattcgttta attccgcccg ctccgctgag gttaagaagc
    29941 caaacccgac catttcatcc agcgatgttg cgccaaaatg acggcgggcc acccattgca
    30001 gagtgtgaat atcgcgcaag ccgccagggc tacttttgat gtctggttca aggttgtagc
    30061 tggtgccatg gtaacgctga tggcgctggt tctgttcttc aactttcgct gcgtagaact
    30121 tgtcggaagg ccagaatcct tcgctaaaaa tatgtttttg cagttcgagg aagagcgcaa
    30181 catcgccaat taataagcgg gattcgatta aattggtggc gacggttaaa tccgataacc
    30241 cttccaacat gcactcttca agcgtgcgca cactatgacc gacttccagc tttacatccc
    30301 agagcagcgt taacagctcg cccacttttt gcgcctgatc gtccgggagc tttttacggc
    30361 ttaaaatcag taaatcgatg tcagaaagtg gatgcagctc gccacgaccg tagccaccga
    30421 cggcaaccaa tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat aatcgttgca
    30481 ggagctggtc tatgaactcg gtacgtgcct caatcaactg ttctgcagag atcccattgt
    30541 caaaggcatc acccagccaa cgctggaaag tatcgatatg ggcttttatc ccacagacgg
    30601 ttaattcatc acggggccag gcgcatggat tttgcggttg accgggcagg gtggagagag
    30661 cggtgtttgc gtactgttct ggaagggtat tcattgtgcg ccacccataa gattaaatta
    30721 tcgcattaaa aaagccggca ttcgccggct tcatcttatt cgtcgtgcga gattatcgcc
    30781 gggatggtgt catccttgcg tagcgtcaaa atttcgcagc cgttatcagt caccacaata
    30841 gtatgctcat attgtgcaga caagctgcga tctttggttt ttaccgtcca gccatctttc
    30901 atggtgcgga tctctttttt acccgcgttg accattggct caatggtgaa cgtcatcccc
    30961 ggtttcagca cgacgttggt ttcacgggag tcatagtgca gcacctgcgg ttcttcatgg
    31021 aagccgcgac caataccgtg tccgcaatat tcacgaacga cggagaagcc ttctgcttcg
    31081 acaaatttct gaatcgccgc accgatttcg cgcagattaa tgcctggttt taccatgcgt
    31141 agcgccaggt acaggctttc ttgcgtgatg cggcacaggc gttcgcccat gatggtcggc
    31201 ttaccgacga taaacatttt cgaggtatcg ccgtggaaac catctttgat tacggtgaca
    31261 tcaatgttaa cgatatcgcc atctttcagc agcttagcat cgtccgggat accgtggcac
    31321 accacttcat taatagagat gcaaacggat ttcggatagc cgtgatagcc gaggcaggca
    31381 gaaaccgcgt gttgttcatt aacaatgtaa tcattacaga tgcgatccag ctcgccggtg
    31441 ctgacgcccg gtttaacata cggttcgatc atctccagca cttcggcagc cagtcggcca
    31501 gcgacgcgca ttttttcgat atcttctggg gtcttgattg agatagccat taattctgtc
    31561 catcagcgtc ggtgataccg acaatatata tgtaagtgcc gtcaatggta tcacacccgg
    31621 gcaaattgag aatcattctg aatttcgcca aacgtgccac tgaaggtttt ctataataga
    31681 aaattcgacg tctgatgctg tacacagcgc caacaattat tggtgtccac gacgtatttg
    31741 tggtataaag cgcgccggac ttccgatcca tttcgtatac acagactgga cggaagcgac
    31801 aatctcactt tgtgtaacaa cacacacgta tcggcacata ttccggggtg ccctttgggg
    31861 tcggtaatat gggatacgtg gaggcataac cccaactttt atatagaggt tttaatcatg
    31921 gcaactgttt ccatgcgcga catgctcaag gctggtgttc acttcggtca ccagacccgt
    31981 tactggaacc cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt tcacatcatc
    32041 aaccttgaga aaactgtacc gatgttcaac gaagctctgg ctgaactgaa caagattgtt
    32101 tctcgcaaag gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga agcggtgaaa
    32161 gacgctgctc tgagctgcga ccagttcttc gtgaaccatc gctggctggg cggtatgctg
    32221 actaactgga aaaccgttcg tcagtccatc aaacgtctga aagacctgga aactcagtct
    32281 caggacggta ctttcgacaa gctgaccaag aaagaagcgc tgatgcgcac tcgtgagctg
    32341 gagaaactgg aaaacagcct gggcggtatc aaagacatgg gcggtctgcc ggacgctctg
    32401 tttgtaatcg atgctgacca cgaacacatt gctatcaaag aagcaaacaa cttgggtatt
    32461 ccggtatttg ctatcgttga taccaactct gatccggacg gtgttgactt cgttatcccg
    32521 ggtaacgacg acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc tgcaaccgta
    32581 cgtgaaggcc gttctcagga tctggcttcc caggcggaag aaagcttcgt agaagctgag
    32641 taataaggct tgataactcc cccaaaatag ttcgagttgc agaaaggcgg caagctcgag
    32701 aattcccggg agcttacatc agtaagtgac cgggatgagc gagcgaagac aacgcatctg
    32761 cggcgcgaaa tatgaagggg gagagccctt atagaccagg tagtacacgt ttggttaggg
    32821 ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc
    32881 acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg
    32941 agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca
    33001 tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag
    33061 gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc
    33121 tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag
    33181 acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac
    33241 agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc
    33301 gcgttgctgc gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggcg
    33361 ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg
    33421 ctgcaagcaa gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag
    33481 aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga
    33541 aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt
    33601 tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga
    33661 ctggcttcat ccgtttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag
    33721 cagaagttgc tgcgatgtcc aagcagtctt aattatcaaa aaggagccgc ctgagggcgg
    33781 cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg
    33841 acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat
    33901 ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct
    33961 gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgtatgg ctcaggaaat
    34021 caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt
    34081 ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat
    34141 gctggcgacc gtaatgaacg gcttggcaat gcgtgatgca ctgcaccgcg cctatgtgaa
    34201 cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga
    34261 agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta caggtaaccc
    34321 gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt
    34381 gctgaaagca accaaagttg acggcgtgtt taccgctgat ccggcgaaag atccaaccgc
    34441 aaccatgtac gagcaattga cttacagcga agtgctggaa aaagagctga aagtcatgga
    34501 cctggcggcc ttcacgctgg ctcgtgacca taaattaccg attcgtgttt tcaatatgaa
    34561 caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga
    34621 ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggcgtaag
    34681 tctggttata aggcgttatt gttgcaggca gtttggtcac ggccagcgcg cagcaaccgg
    34741 agcgtacaaa agtacgtgag gatggcgagc actgctcggg gccaaaatgg caaataaaat
    34801 agcctaataa tccagacgat tacccgtaat atgtttaatc agggctatac ttagcacact
    34861 tccactgtgt gtgactgtct ggtctgactg agacaagttt tcaaggattc gtaacgtgat
    34921 tagcgatatc agaaaagatg ctgaagtacg catggacaaa tgcgtagaag cgttcaaaac
    34981 ccaaatcagc aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg gcattgtcgt
    35041 ggaatattac ggcacgccga cgccgctgcg tcagctggca agcgtaacgg tagaagattc
    35101 ccgtactctg aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg aaaaagcgat
    35161 tatggcctcc gatctcggcc tgaacccgaa ctctgcgggt agcgacatcc gtgttccgct
    35221 gccgccgctg acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg gtgaagcaga
    35281 acaagcgcgt gttgcagtac gtaatgtgcg tcgtgacgcg aacgacaaag tgaaagcact
    35341 gttgaaagat aaagagatca gcgaagacga cgatcgccgt tctcaggacg atgtacagaa
    35401 actgactgat gctgcaatca agaaaattga agcggcgctg gcagacaaag aagcagaact
    35461 gatgcagttc tgatttcttg aacgacaaaa acgccgctca gtagatcctt gcggatcggc
    35521 tggcggcgtt ttgcttttta ttctgtctca actctggatg tttcatgaag caactcacca
    35581 ttctgggctc gaccggctcg attggttgca gcacgctgga cgtggtgcgc cataatcccg
    35641 aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg gtagaacagt
    35701 gcctggaatt ctctccccgc tatgccgtaa tggacgatga agcgagtgcg aaacttctta
    35761 aaacgatgct acagcaacag gggagccgca ccgaagtctt aagtgggcaa caagccgctt
    35821 gcgatatggc agcgcttgag gaggttgatc aggtgatggc agccattgtt ggtgctgctg
    35881 ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac cattttgctg gccaataaag
    35941 aatcactggt tacctgcgga cgtctgttta tggacgccgt aaagcagagc aaagcgcaat
    36001 tgttaccggt cgatagcgaa cataacgcta tttttcagag tttaccgcaa cctatccagc
    36061 ataatctggg atacgctgac cttgagcaaa atggcgtggt gtccatttta cttaccgggt
    36121 ctggtggccc tttccgtgag acgccattgc gcgatttggc aacaatgacg cccgatcaag
    36181 cttgccgtca tccgaactgg tcgatgggac gtaaaatttc tgtcgattcg gctaccatga
    36241 tgaacaaagg tctggaatac attgaagcgc gttggctgtt taacgccagc gccagccaga
    36301 tggaagtgct gattcacccg cagtcagtga ttcactcaat ggtgcgctat caggacggca
    36361 gtgttctggc gcagctgggg gaaccggata tgcgtacgcc aattgcccac accatggcat
    36421 ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta agtgcgttga
    36481 catttgccgc accggattat gagcgttatc catgcctgaa actggcgatg gaggcgttcg
    36541 aacaaggcca ggcagcgacg acagcattga atgccgcaaa cgaaatcacc gttgctgctt
    36601 ttcttgcgca acaaatccgc tttacggata tcgctgcgtt gaatttatct gtactggaaa
    36661 aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat gcgaacgcga
    36721 gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc ggctacagag
    36781 agtcgcgcta tttgttagcg tagggcttca atgatatagt ctgcgccatc tgatcgtaag
    36841 tagttggctt tataaggtca ggtatgccgt ggttttacac ggcttttttt tgtataggct
    36901 tcagtattcc tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga tgttgtctgc
    36961 tactcaacca cttagcgaaa aattgccagc gcatggctgc cgtcatgttg cgatcattat
    37021 ggacggcaat ggtcgctggg caaaaaagca agggaagatt cgtgcctttg ggcataaagc
    37081 cggggcaaaa tccgtccgcc gggctgtctc ttttgcggcc aacaacggta ttgaagcgtt
    37141 aacgctgtat gcctttagta gtgaaaactg gaaccgacca gcgcaggaag tcagtgcgtt
    37201 aatggaactg tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc gacataacgt
    37261 gcgtctgcgt attattggcg ataccagtcg ttttaactcg cgtttgcaag aacgtattcg
    37321 taaatctgaa gcgctaacag ccggaaatac cggtctgacg ctgaatattg cggcgaacta
    37381 cggtggacgt tgggatatag tccagggagt caggcaactg gctgaaaagg tgcagcaagg
    37441 aaacctgcaa ccagatcaga tagatgaaga gatgttaaac cagcatgtct gtatgcatga
    37501 actggcccct gtagatttag taattaggac tgggggagag catcgcatta gtaacttttt
    37561 gctttggcaa attgcctatg ccgaacttta ctttacagat gttctctggc ccgatttcga
    37621 tgaacaagac tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc gtttcggcgg
    37681 caccgagccc ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt atcgcctgat
    37741 atctgctttt gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc cgccggtggg
    37801 gttcgccatt gtaacgctgg tggtctgcat gctggcagcg tgggaatggg gacagcttag
    37861 cggttttacc actcgttcgc agcgagtatg gttggcggtg ttatgcgggt tattgttggc
    37921 gctgatgctt tttctgttgc cggaatatca ccgaaatatt catcaaccgc tggttgaaat
    37981 ctcactttgg gcttcgttgg gttggtggat tgtcgcgcta ttgctggtgc tgttttaccc
    38041 aggttccgca gcaatctggc gtaactctaa aacattgcgc cttatttttg gcgtgctaac
    38101 cattgttccc ttcttctggg gcatgctggc gttacgggcc tggcactatg acgagaatca
    38161 ttacagtggc gcaatatggc tgctctatgt catgatcctg gtatggtgcg ctgactccgg
    38221 cgcatatatg tttggcaaat tgtttggtaa acataagctg gcaccgaagg tttctccggg
    38281 taaaacctgg caaggcttta taggtggact cgctactgca gcggtaatct catggggtta
    38341 tggcatgtgg gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct ctattgtcgc
    38401 agcgttagcc tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg aagcaggaat
    38461 taaggacagc ggtcatttaa ttccaggaca cggtggtatt ttagatcgta ttgatagcct
    38521 gacggctgcg gtaccggtct ttgcttgctt gttgttactg gtattcagga cgctttaacg
    38581 gaaggtaata tgctgagttt tctctgggat ttggcttcgt tcatcgttgc actgggtgta
    38641 cttatcaccg tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg tgttcgcgtt
    38701 gagcgtttct caatagggtt tggtaaggcg ctctggcggc gaactgataa gctcggcacc
    38761 gaatatgtta tcgccctgat cccgttgggc ggttatgtca aaatgctgga tgagcgcgca
    38821 gaaccggtcg ttccggaact ccgccaccat gccttcaata ataaatctgt cggccaacga
    38881 gtggcgatta ttgccgcagg tccggttgca aacttcattt ttgctatctt tgcctactgg
    38941 ctggttttta ttattggtgt gcctggcgta cgtccggtgg ttggcgaaat agcagccaat
    39001 tcgatagctg cggaagcaca aattgcacca ggtacggaac taaaagccgt agatggtatc
    39061 gaaacgcctg attgggatgc cgtgcgtttg cagttggtcg ataaaattgg cgatgaaagc
    39121 accaccatta cagtagcgcc atttggcagc gaccaacggc gggatgtaaa gctcgattta
    39181 cgtcactggg cgtttgagcc tgataaagaa gatccggtat cttcgctggg gattcgtcct
    39241 cgtgggccgc aaattgaacc tgtactggaa aatgtgcagc caaactcggc ggcaagcaag
    39301 gcaggtttgc aagcaggcga caggatcgtt aaagtcgatg gtcagccctt aacgcagtgg
    39361 gtgacctttg tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt agaaatcgaa
    39421 aggcagggga gtcccttgtc tttgacatta atcccggaga gtaaaccggg taatggtaaa
    39481 gcgattggtt ttgtcggtat tgagccgaaa gtcattcctt tgccagatga gtataaagtt
    39541 gtacgccagt atgggccgtt caacgccatc gtcgaagcca cggacaaaac gtggcagctg
    39601 atgaagctga cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa actgaacaac
    39661 ctcagtgggc cgatttctat cgccaagggg gctgggatga cagcggaact cggggtagtt
    39721 tattacctgc cgtttcttgc gcttattagc gtgaacttag ggataattaa cctgtttccg
    39781 ttgcccgtac ttgacggggg gcatctgctg ttccttgcga tcgaaaagat caagggcgga
    39841 ccggtatccg agcgggttca agacttttgt tatcgcattg gctcgattct gctggtgctg
    39901 ttaatggggc ttgcactttt caatgatttc tctcggttat gagagttagt taggaagaac
    39961 gcataataac gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt agcagcgcca
    40021 ccgtatacgg tgctgaaggg ttcgtagtga aagatattca tttcgaaggc cttcagcgtg
    40081 tcgccgttgg tgcggccctc ctcagtatgc cggtgcgtac aggcgacacg gttaatgatg
    40141 aagatatcag taataccatt cgcgctctgt ttgctaccgg caactttgag gatgttcgcg
    40201 tccttcgtga tggtgatacc cttctggttc aggtaaaaga acgtccgacc attgccagca
    40261 ttactttctc cggtaacaaa tcggtgaaag atgacatgct aaagcaaaac ctcgaggctt
    40321 ctggtgtgcg tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc gagaaaggtc
    40381 tggaagactt ctactacagc gtcggtaaat atagcgccag cgttaaagct gtcgtgaccc
    40441 cgctgccgcg caaccgtgtt gacctaaaac tggtgttccg ggaaggtgtg tcagctgaaa
    40501 tccagcaaat taacattgtt ggtaaccatg ctttcaccac cgacgaactg atctctcatt
    40561 tccaactgcg tgacgaagtg ccgtggtgga atgtggtagg cgatcgtaaa taccagaaac
    40621 agaaactggc gggcgacctt gaaaccctgc gcagctacta tctggaccgc ggttatgccc
    40681 gtttcaacat cgactctacc caggtcagtc tgacgccaga taaaaaaggt atttacgtca
    40741 cggtgaacat caccgaaggc gatcagtaca agctttctgg cgttgaagtg agcggcaacc
    40801 ttgccgggca ctccgctgaa attgagcagc tgactaagat cgagccgggc gagctgtata
    40861 acggcactaa agtgaccaaa atggaagatg acattaaaaa gcttctcggt cgctatggtt
    40921 atgcctatcc gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa accgttaaat
    40981 tacgcgtgaa cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt tttgaaggta
    41041 acgatacctc gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa ggtgcatggt
    41101 tggggagcga tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc ttctttgaaa
    41161 ctgtcgatac tgatacccaa cgtgttccgg gtagcccgga ccaggttgat gtagtctaca
    41221 aggtgaaaga acgtaacact ggtagcttca acttcgggat tggttacggt actgaaagtg
    41281 gcgtgagctt ccaggctggc gtgcaacagg ataactggtt aggtacaggt tatgctgttg
    41341 gtatcaacgg gaccaaaaac gattaccaga cctatgctga actgtcggta accaacccgt
    41401 acttcaccgt agatggcgta agcctcggtg gtcgtctctt ctataatgac ttccaggcag
    41461 atgacgccga cctgtccgac tataccaaca agagttatgg tacagacgtg acgttgggct
    41521 tcccgattaa cgaatataac tcgctgcgtg caggtctggg ttatgtacat aactccctgt
    41581 ccaacatgca gcctcaggtt gcgatgtggc gttatctgta ctctatgggt gaacatccga
    41641 gcacttctga tcaggataac agcttcaaaa cggacgactt cacgttcaac tatggttgga
    41701 cctataacaa gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc aacctgaccg
    41761 gtaaagtgac cattcctgga tcggataacg aatactacaa agtgacgtta gacacggcga
    41821 cttatgtgcc gatcgatgac gatcacaaat gggttgttct gggtcgtacc cgctggggtt
    41881 atggtgatgg tttaggcggc aaagagatgc cgttctacga gaacttctat gccggtggtt
    41941 ccagcaccgt gcgtggcttc cagtccaata ccattggtcc gaaagcagtt tacttcccgc
    42001 atcaggccag taattatgat ccggactatg attacgaatg tgcgactcag gacggcgcga
    42061 aagacctgtg taaatcggat gatgctgtag gcggtaacgc catggcggtt gccagcctcg
    42121 agttcatcac cccgacgccg tttattagcg ataagtatgc taactcggtt cgtacttcat
    42181 tcttctggga tatgggtacc gtttgggata caaactggga ttccagccaa tattctggat
    42241 atccggacta tagtgatcca agcaatatcc gtatgtctgc gggtatcgca ttacaatgga
    42301 tgtccccatt ggggccgttg gtgttctcct acgcccagcc gttcaaaaag tacgatggag
    42361 acaaggcaga acagttccag tttaacatcg gtaaaacctg gtaagtgttc tccacaaagg
    42421 aatgtagtgg tagtgtagcg atgactttag gcgatcaata taagatcgcc gggccacgca
    42481 aagaactgca ccctccggtg caaatgggat ggtaaggagt ttattgtgaa aaagtggtta
    42541 ttagctgcag gtcttggttt agcactggca acttctgctc aggcggctga caaaattgca
    42601 atcgtcaata tgggcagcct gttccagcag gtagcgcaga aaaccggtgt ttctaacacg
    42661 ctggaaaatg agttcaaagg ccgtgccagc gaactgcagc gtatggaaac cgatctgcag
    42721 gctaaaatga aaaaactgca gtccatgaaa gcgggcagcg atcgcaccaa actggaaaaa
    42781 gacgtgatgg ctcagcgcca gacttttgct cagaaagcgc aggcttttga gcaggatcgc
    42841 gcacgtcgtt ccaacgaaga acgcggcaaa ctggttactc gtatccagac tgctgtgaaa
    42901 tccgttgcca acagccagga tatcgatctg gttgttgatg caaacgctgt tgcttacaac
    42961 agcagcgatg taaaagacat cactgccgac gtactgaaac aggttaaata agtaatgcct
    43021 tcaattcgac tggctgattt agcgcagcag ttggatgcag aactacacgg tgatggcgat
    43081 atcgtcatca ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat tacgttcatg
    43141 gttaacccaa aataccgtga gcatttaggc ttgtgccaag cgtccgcggt tgtcatgacc
    43201 caggacgatc ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc ctacctgact
    43261 tacgcgcgca tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa cattgcaccc
    43321 agtgcggtga tcgacgcgac ggcgaagctg ggtaacaacg tatcgattgg cgctaacgcg
    43381 gtgattgagt ccggcgttga actgggcgat aacgtgatta tcggtgccgg ttgcttcgta
    43441 ggtaaaaaca gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac catttaccat
    43501 gagatccaga tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg cgcagacggc
    43561 tttggttatg ccaacgatcg tggtaactgg gtgaagatcc cacagattgg tcgcgtaatt
    43621 attggcgatc gagtggagat cggtgcctgc acaaccatcg atcgcggcgc gctggatgac
    43681 actgttattg gcaatggcgt gatcattgat aaccagtgcc agattgcaca taacgtcgtg
    43741 attggcgaca atacggcggt tgccggtggc gtcattatgg cgggcagcct gaaaattggt
    43801 cgttactgca tgatcggcgg agccagcgta atcaacgggc atatggaaat atgcgacaaa
    43861 gtgacggtta cgggcatggg tatggtgatg cgtcccatca ctgaaccagg cgtctattcc
    43921 tcaggcattc cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact ggtgatgaac
    43981 attgatgaca tgagcaagcg tctgaaatcg cttgagcgca aggttaatca acaagactaa
    44041 cgttccatct tttgttcgcc aaactttacg gcctgtctca ttcttacgat tgcggcaggc
    44101 cgtgttatta ttgtcgtttc ttatattttg acaggaagag tatcttgact actaacactc
    44161 atactctgca gattgaagag attttagaac ttctgccgca ccgtttcccg ttcttactgg
    44221 tggatcgcgt gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa aatgtctctg
    44281 tcaatgagcc attcttccag ggccatttcc ctggaaaacc gattttcccg ggtgtgctga
    44341 ttctggaagc aatggcacag gcaacaggta ttctggcgtt taaaagcgta ggaaaactgg
    44401 aaccgggtga gctgtactac ttcgctggta ttgacgaagc gcgcttcaag cgcccggtcg
    44461 tgcctggcga tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc ggcctgaccc
    44521 gttttaaagg ggttgctctg gtcgatggta aagtagtttg cgaagcaacg atgatgtgtg
    44581 ctcgtagccg ggaggcctga tacgtgattg ataaatccgc ctttgtgcat ccaaccgcca
    44641 ttgtggaaga gggcgcgtca attggcgcga acgcacacat tggtcctttt tgtatcgttg
    44701 gaccccatgt cgaaattggt gagggtaccg tactgaaatc tcacgttgtc gtgaatggtc
    44761 atactaaaat tggccgcgat aatgagattt atcagtttgc ctccatcggc gaagttaacc
    44821 aggatttgaa atatgctggc gaaccgaccc gtgtggaaat cggcgatcgt aaccgcattc
    44881 gcgaaagcgt caccattcat cgtggcacag tccagggcgg tggattgacg aaggtgggca
    44941 gcgacaactt actgatgatc aacgcgcaca ttgcgcacga ttgtacggta ggtaaccgct
    45001 gtattctcgc caacaacgca acgctggcgg gtcacgtatc ggttgacgac ttcgcgatca
    45061 tcggcggcat gaccgcagtc catcagttct gcatcattgg tgcgcatgtg atggttggcg
    45121 gctgctccgg tgtggcgcag gacgtccctc cttatgtcat tgcgcagggt aaccacgcaa
    45181 cgccgttcgg tgtcaatatc gaagggctga agcgccgcgg attcagccgt gaggcgatta
    45241 ccgctatccg caatgcgtat aagctaattt atcgtagcgg taaaacgctc gatgaagtga
    45301 aaccggaaat tgctgaactg gcggaaacat atccggaagt gaaagccttt accgatttct
    45361 ttgcacgctc aacgcgcggt ctgattcgtt aatgactgac cagcgtccat taacgattgc
    45421 cctggtcgcc ggagaaatct ccggcgatat cctgggggcc ggtttaatcc gcgctctgaa
    45481 agaacgtgtg cccaacgccc gctttgttgg tgttgccggg ccacgaatgc aggctgaagg
    45541 ctgcgaagcc tggtacgaaa tggaagaact ggcggtgatg ggcattgttg aagtgctcgg
    45601 tcgtctgcgt cgcttactgc atattcgtgc cgatctgaca aagcgttttg gcgaactgaa
    45661 gccagatgtt tttgttggta ttgatgcgcc cgacttcaat attactcttg aaggtaacct
    45721 caaaaagcag ggtatcaaaa ccattcatta cgtcagtcca tccgtctggg cgtggcgaca
    45781 gaaacgcgtt ttcaaaatag gcagagccac cgatctggtg ctcgcatttc tgcctttcga
    45841 aaaagcgttt tatgacaaat acaatgtacc gtgccgcttt atcggtcata ccatggctga
    45901 tgccatgccg ttagatccag ataaaaatgg tgcccgtgat gtgctgggga tcccttacga
    45961 tgcccactgt ctggcattgt tgccgggcag ccgtggtgca gaagtcgaaa tgcttagtgc
    46021 cgatttcttg aaaactgccc agcttttgcg ccagacatat ccggatctcg aaatcgtggt
    46081 gccgctggtg aatgccaaac gccgcgagca gtttgaacgc atcaaagctg aagtcgcgcc
    46141 agacctgtcg gttcatttgc tggatggaat gggccgtgag gcgatggtcg ccagcgatgc
    46201 ggcactactg gcgtcgggga cggcagccct ggagtgtatg ctggcgaaat gcccgatggt
    46261 ggtgggatat cgcatgaagc cttttacctt ctggttggcg aagcggctgg tgaaaactga
    46321 ttatgtctcg ctgccaaatc tgctggcggg cagagagtta gtcaaagagt tattgcagga
    46381 agagtgtgag ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga acgggaaaac
    46441 cagccacgcg atgcacgata ccttccgtga actgcatcag cagatccgct gcaatgccga
    46501 tgagcaggcg gcacaagccg ttctggagtt agcacaatga tcgaatttgt ttatccgcac
    46561 acgcagctgg ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg cgcggtcgtc
    46621 accgctgcgg tgatccttga cccggcgcgc ccgattgccg ggctgaatga ttccaaaaag
    46681 ctgagcgaaa aacgccgtct ggcgctctat gaagagatca aagagaaagc gttgagctgg
    46741 agtctgggcc gcgcggaacc ccacgaaatc gacgagctga acattcttca tgcgaccatg
    46801 ctggcgatgc agcgtgccgt cgctgggctg catattgcgc cggaatatgt gttgattgat
    46861 ggtaaccgct gcccgaaatt accgatgcct gcgatggctg tggtgaaagg ggatagccgc
    46921 gtaccagaaa tcagtgccgc gtctatccta gcgaaagtga cgcgtgacgc cgaaatggcg
    46981 gcgctggata ttgttttccc gcaatatggt tttgcccaac ataaagggta tccaaccgct
    47041 tttcatctgg aaaaactggc tgaatatggc gcgactgaac atcatcgtcg cagctttggg
    47101 cctgtcaaac gcgcactggg acttgcgtcc tgattcttgt gtcgagatta agtaaaccgg
    47161 aatctgaaga tgtctgaacc acgtttcgta cacctgcggg tgcacagcga ctactcgatg
    47221 atcgatggct tggccaaaac cgcaccgctg gtaaaaaagg cggcggcgtt gggtatgcca
    47281 gcactggcga tcaccgattt caccaacctt tgcggtctgg tgaagttcta cggagcggga
    47341 catggcgcag ggattaagcc tatcgtcggg gcagatttta atgtccagtg cgacctgctg
    47401 ggtgatgagt taacccacct gacggtactg gcggcgaaca ataccggcta tcagaatctg
    47461 gcgttgctga tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc gatcatcgat
    47521 cgcgactggc ttatcgaatt aaacgaaggg ttgatccttc tttccggcgg gcgcatgggc
    47581 gacgtcggac gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg tgtcgcgttt
    47641 tatgaagaac acttcccgga tcgctatttt ctagagctga tccgcaccgg caggccggat
    47701 gaagaaagct atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt gcccgtcgtg
    47761 gcgactaacg atgtgcgctt tatcgacagc agcgactttg acgcacacga aatccgcgtc
    47821 gcgatccacg acggctttac cctcgacgat cctaaacgcc cgcgtaacta ttcgccgcag
    47881 caatatatgc gtagcgaaga ggagatgtgt gagctgtttg ccgacatccc agaagccctt
    47941 gccaacaccg ttgagatcgc caaacgctgt aacgtaactg tgcgtcttgg tgaatacttc
    48001 ctgccgcagt tcccgaccgg ggacatgagc accgaagatt atctggtcaa gcgtgcaaaa
    48061 gaaggcctgg aagagcgtct ggcattttta ttccctgacg aggaagaacg tcttaaacgc
    48121 cgcccggaat atgacgaacg tctggagact gaacttcagg ttatcaacca gatgggtttc
    48181 ccgggctact tcctcatcgt tatggaattt atccagtggt cgaaagataa cggcgtaccg
    48241 gtaggaccag gccgtggctc cggtgcgggt tcactggtgg cctacgcgct gaaaatcacc
    48301 gacctcgatc cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc ggaacgtgtc
    48361 tccatgcctg acttcgacgt tgacttctgt atggagaaac gcgatcaggt tatcgaacac
    48421 gtggcggaca tgtacggtcg tgatgcggta tcgcagatta tcaccttcgg tacgatggcg
    48481 gcgaaagcgg tgatccgcga cgtaggccgc gtgctggggc atccgtacgg ctttgtcgac
    48541 cgtatctcga aactgatccc gcccgatccg gggatgacgc tggcgaaagc gtttgaagcc
    48601 gagccgcagc tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct gatcgacatg
    48661 gcgcgcaaac tggaaggggt cacccgtaac gccggtaagc acgccggtgg ggtggttatc
    48721 gcgccgacca aaattaccga ttttgcgccg ctttattgcg atgaagaggg caaacatccg
    48781 gtcacccagt ttgataaaag cgacgttgaa tacgccggac tggtgaagtt cgacttcctt
    48841 ggtttgcgta cgctcaccat catcaactgg gcgctggaga tgatcaacaa gcggcgggcg
    48901 aagaatggcg agccaccgct ggatatcgcc gcgatcccgc tggatgacaa gaaaagcttc
    48961 gacatgctgc aacgctcgga aaccacggcg gtattccagc ttgaatcgcg cggcatgaag
    49021 gacctgatca agcgtctgca acctgactgc ttcgaagata tgatcgcact ggtggcactg
    49081 ttccgccctg gtccgttgca gtcagggatg gtggataact ttatcgaccg taaacatggt
    49141 cgtgaagaga tctcctatcc ggacgtgcag tggcagcatg aaagcctgaa atcggtactg
    49201 gagccaacct acggcattat cttgtatcag gaacaggtca tgcagattgc ccaggtgctt
    49261 tctggttata ccctcggtgg cgcggatatg ctgcgccgtg cgatgggtaa gaaaaagccg
    49321 gaagagatgg ctaagcagcg ttctgtattt gctgaaggtg cagaaaagaa cggaatcaac
    49381 gccgaactgg cgatgaaaat cttcgacctg gtggagaaat tcgcgggtta cggatttaac
    49441 aaatcgcact ctgcggccta tgctttggtg tcatatcaaa cgttatggct gaaagcgcac
    49501 tatccggcgg agtttatggc ggcggtaatg accgccgata tggacaacac cgagaaggtg
    49561 gtgggcctgg tggatgagtg ctggcggatg gggctgaaaa tcctgccacc agatataaac
    49621 tccggtcttt accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg tattggcgcc
    49681 atcaaagggg taggtgaagg tccgattgag gccatcatcg aagcccgtaa taaaggcggc
    49741 tacttccgcg aactgtttga tctctgcgcc cgtaccgaca ccaaaaagtt aaaccggcgg
    49801 gtgctggaaa aactgatcat gtccggggcg tttgaccgtc ttgggccaca ccgcgcggcg
    49861 ctgatgaact cgctgggtga tgcgttaaaa gcggcagatc aacacgcgaa agcggaagct
    49921 atcggtcagg ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat tgaacaatcc
    49981 tacgccagct gccaaccgtg gccggagcag gtggtattag atggggaacg tgaaacgtta
    50041 ggcctgtacc tgaccggaca ccctatcaac cagtatttaa aagagattga gcgttatgtc
    50101 ggaggcgtaa ggctgaaaga catgcacccg acagaacgtg gtaaagtcat cacagctgcg
    50161 gggctcgttg ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat cggtatctgc
    50221 acgctggatg accgttccgg gcggctggaa gtgatgttgt ttactgacgc cctggataaa
    50281 taccagcaat tgctggaaaa agaccgcata cttatcgtca gcggacaggt cagctttgat
    50341 gacttcagcg gtgggcttaa aatgaccgct cgcgaagtga tggatattga cgaagcccgg
    50401 gaaaaatatg ctcgcgggct tgctatctcg ctgacggaca ggcaaattga tgaccagctt
    50461 ttaaaccgac tccgtcagtc tctggaaccc caccgctctg ggacaattcc agtacatctc
    50521 tactatcaga gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg gcgtgtctct
    50581 ccgagcgatc gtttattaaa cgatctccgt ggcctcattg gttcggagca ggtggaactg
    50641 gagtttgact aatacaggaa tactatgagt ctgaatttcc ttgattttga acagccgatt
    50701 gcagagctgg aagcgaaaat cgattctctg actgcggtta gccgtcagga tgagaaactg
    50761 gatattaaca tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga actgacgcgt
    50821 aaaatcttcg ccgatctcgg tgcatggcag attgcgcaac tggcacgcca tccgcagcgt
    50881 ccttataccc tagattacgt tcgcctggca tttgatgaat ttgacgaact ggctggcgac
    50941 cgtgcgtatg cagacgataa agctatcgtc ggtggtatcg cccgtctcga tggtcgtccg
    51001 gtgatgatca ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg ccgtaacttt
    51061 ggtatgccag cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat ggctgaacgc
    51121 tttaagatgc ctatcatcac ctttatcgac accccggggg cttatcctgg cgtgggcgca
    51181 gaagagcgtg gtcagtctga agccattgca cgcaacctgc gtgaaatgtc tcgccttggc
    51241 gtaccggtag tttgtacggt tatcggtgaa ggtggttctg gcggtgcgct ggcgattggc
    51301 gtgggcgata aagtgaatat gctgcaatac agcacctatt ccgttatctc gccggaaggt
    51361 tgtgcgtcca ttctgtggaa gagcgccgac aaagcgccgc tggcggctga agcgatgggt
    51421 atcatcgctc cgcgtctgaa agagctgaaa ctgatcgact ccatcatccc ggaaccgctg
    51481 ggcggcgctc accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca actgctggcg
    51541 gatctggccg atctcgatgt gttaagcact gaagatttaa aaaatcgtcg ttatcagcgc
    51601 ctgatgagct acggttacgc gtaattcgca aaagttctga aaaagggtca cttcggtggc
    51661 ccttttttat cgccacggtt tgagcaggct atgattaagg aaggattttc caggaggaac
    51721 acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat gagcccatca
    51781 aagaactgga gtcggcgctg gtggcgcaag gctttcagat tatctggcca caaaacagcg
    51841 ttgatttgct gaaatttatc gaacataacc ctcgaatttg cggcgtgatt tttgactggg
    51901 atgagtacag tctcgattta tgcagcgata tcaatcaact taatgaatat ctcccgcttt
    51961 atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg cggatggcgc
    52021 tctggttttt tgaatatgcg ctggggcagg cggaagatat cgccattcgt atgcgtcagt
    52081 acaccaacga atatcttgat aacattacgc cgccgttcac gaaagccttg tttacctacg
    52141 tcaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggcacc gcatatcaaa
    52201 aaagcccggt tggctgtctg ttttatgatt ttttcggcgg gaataccctt aaggctgatg
    52261 tctctatttc ggtcaccgag cttggttcgt tgcttgacca caccgggcca cacctggaag
    52321 cggaagagta catcgcgcgg acttttggcg cggaacagag ttatatcgtt accaacggga
    52381 catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt acgctgttga
    52441 tcgaccgcaa ctgccataaa tcactggcgc atctgttgat gatgaacgat gtagtgccag
    52501 tctggctgaa accgacgcgt aatgcgttgg ggattctggg ggggatcccg cgccgtgaat
    52561 ttactcgcga cagcatcgaa gagaaagtcg ctgccactac gcaagcacaa tggccggttc
    52621 atgcggtgat caccaactcc acctatgatg gtttgctcta caacaccgac tggatcaaac
    52681 agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtat actcattttc
    52741 atccaatcta ccagggtaaa agtggtatga gcggcgagcg tgttgcggga aaagttttct
    52801 tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttca ctgatccaca
    52861 ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat accaccacct
    52921 cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg cgtggtaatc
    52981 cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc aaagaggtcc
    53041 agcggctgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg ccgcaggtgg
    53101 atgaagccga atgctggccc gttgcgcctg gcgaacaatg gcacggcttt aacgatgcgg
    53161 atgccgatca tatgtttctc gatccggtta aagtcactat tttgacaccg gggatggacg
    53221 aacagggcaa tatgagtgag gaggggatcc cggcggcgct ggtagcaaaa ttcctcgacg
    53281 aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc tttagtattg
    53341 gcatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt aagcgctctt
    53401 acgatctcaa cctgcggatc aaaaatatgc tgcccgatct ctatgctgaa gatcccgatt
    53461 tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg attcgtaaac
    53521 acgatctttc cggtttgatg ttgcgggcat tcgatacttt gccggaaatg atcatgacgc
    53581 cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg ctggaacaac
    53641 tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta ccgctgttga
    53701 tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta ctgatgcttt
    53761 gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg aaacaggacg
    53821 aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc agagcggctt
    53881 ctgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt taaaacaggt
    53941 tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct actgcgatat
    54001 tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt ggaaagggga
    54061 tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc cgccggagcg
    54121 ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg atgacatcga
    54181 tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc gtgtcgatcc
    54241 atacacgcaa aaacgcttca ccttctttaa cgatccggac gggctgccgt tggaactgta
    54301 tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca ttgctactgt
    54361 aaaatcgcac catcatgaca ctcacgctca atagaccact tctcacctca cgccagattc
    54421 tggtggcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg gtgcagtggc
    54481 ggacggaaaa tccgggcgtc actctgcgcg ctattcatgt gcatcacggt ttaagtgcca
    54541 atgccgatgc ctgggttacg cattgcgaaa acgtctgcca acagtggcag gtgccgctgg
    54601 tggtcgaacg cgtacaactt gcgcaagagg ggctggggat tgaggcccag gcgcggcagg
    54661 cgcgttatca ggcatttgcc cgtaccctat tgcccggcga agtgctggtc accgcgcaac
    54721 atctcgacga tcaatgtgaa acctttctgc tggcgctaaa acgcggtagc ggccctgccg
    54781 ggctttcggc tatggcggaa gtttcggagt ttgctggaac acggcttatc cgcccgttgc
    54841 ttgcccgcac gcggggggaa ctggagcagt gggcattggc gcatggttta cgctggattg
    54901 aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg cgcgtagtgc
    54961 cgttattgca gcagcgttgg ccgcattttg cggaagcaac ggcccgcagc gccacacttt
    55021 gtgctgaaca agagagtctg ttggatgaac tgctggcgga tgatttagca cactgccaaa
    55081 cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc cgtcgcgcgg
    55141 cgattattcg ccgctggctg gcagggcaga atgcaccgat gccttcccgc gacgcgctgg
    55201 tgcgcatctg gcaggaagtg gcgctggcgc gggaagatgc ttccccctgt ttacgtttgg
    55261 gcgcctttga aatccgccgc tatcagtcgc aactgtggtg gattaaatcc gtcaccgggc
    55321 aaagcgaaac cattgtgctg tggcagacgt ggctacaacc gctggaatta ccggcggggc
    55381 tgggaaccgt acagcttact gcgggaggcg atattcgccc tccgcgtgca gacgaagcgg
    55441 tcagcgtgcg tttcaaagcg ccaggattgc tgcatattgt cgggcgcaac ggcggacgta
    55501 agctgaagaa aatctggcaa gagttaggcg tgccgccgtg gctacgtgac accacgccac
    55561 tgctgtttta tggagaaacg ctgattgcgg cggcaggggt atttgtgacg caagaaggtg
    55621 tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt taagtgaaag
    55681 ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggattc gcttaccacc
    55741 accgtaccga tttcagggtg gctaaagctg gtaattttat ccagacgcag ctcgcgggtt
    55801 tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa atcactggct
    55861 tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg atgctggcag
    55921 gcgagctcaa gattatcgta atcatcacaa ttaattggtt gatacgtatc attcattgac
    55981 ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga cagcctcaga
    56041 aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca gcgcgtccgg
    56101 aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta actcacaata
    56161 tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag tctaagccta
    56221 cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca atatcagcgc
    56281 cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt tcttatggcg
    56341 cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg caaccagttt
    56401 ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg tatgatgctg
    56461 cgcttgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg tggtttgtgt
    56521 gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga tttgtggatt
    56581 gagctggggc tgccggatga gcgacgaatt aagaaagcct gcacccaggc cgcagaagtg
    56641 gcgctgttta cctataatag tcgggcggcg caaatctggt ggcagcaaaa tcagagcaaa
    56701 tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact ggcgaaagta
    56761 agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg tgttatctgg
    56821 ttatcggatg ataagaataa tctggaagtt aacttaaccg cctggtaaca accttcatga
    56881 ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc gccattcgtg
    56941 cgcagggcgc tggcgggcag catgttaata agacctcaac ggctattcat ctgcgttttg
    57001 acattcgggc gcccagcctg ccagagtatt acaaagagcg tctgctcgcc gccagccatc
    57061 atttgatcag cagtgatggc gtgattgtca ttaaggcaca ggaataccgc agtcaggaac
    57121 tgaaccgcga agccgctctg gcccggttgg tggcagtgat taaagattta acaaaagaac
    57181 aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa agagcgcagg ctggcatcga
    57241 aagcacaaaa atcaagcgtg aaggcgatgc gcggcaaagt acgcagcggt cgggaataaa
    57301 aagaaggaat ggatggtgaa aaaagcgata gtgacagcga tggctgtaat cagcctcttt
    57361 actctgatgg gatgtaataa tcgggccgaa gtcgatacgc tttctccggc gcaggctgcc
    57421 gaactgaaac cgatgccgca aagttggcgc ggcgtgctac cgtgtgccga ttgcgaagga
    57481 atcgaaatct ctctgttcct cgaaaaagac ggaacatggg tgatgaatga gcgttatctc
    57541 ggtgctcgtg aagaaccttc ctccttcgct tcctacggta catgggcgcg aaccgctgac
    57601 aagctggtat taaccgacag caaaggtgaa aagtcatatt atcgggcgaa aggcgatgcg
    57661 ctggagatgc tcgatcgtga aggcaatcca attgaatcgc agttcaacta tacgctggaa
    57721 ccggcacaat ccagtttacc tatgacgccg atgaccctgc gaggcatgta tttttatatg
    57781 gctgatgcgg cgaccttcac tgattgcgcg accggaaaac gtttcatggt agcgaataac
    57841 gcagagctgg agcgtagcta cctggctgcg cgcggtcaca gtgaaaaacc ggtgttactg
    57901 tcagtagaag gtcactttac gcttgaggct aatccggata ctggtgcgcc aaccaaagta
    57961 ttagcgcccg atacggcggg taaattttac ccgaataagg attgcagtag tttggggcag
    58021 taaacctgct taaggcagaa acaaacgcaa aattgcctga tgcgctgcgc ttatcaggcc
    58081 tacagcatat aatgcaacat tttgaattag cacgattttg taggccggat aaggcgttta
    58141 cgccgcatcc ggcattttgt gcacgaggct ggctttatca ctgagtcagc ccaacaaagc
    58201 gacccgcctt gatataaatc ccaggcttca ggtgcccaac atactgtaac tgcgtttctg
    58261 gcttaaacgc agagacatca ccaatacgca tatgcatcag atcggcaggg ctaatccagc
    58321 ccgattgcgc cagtgtaagt ggatgcccgg ctttggcaaa tgcatcagtg acaaattccg
    58381 aacaaaacca cgactttttg tctccttcgc acacactgct taactgcgct ttcgccaggt
    58441 cgctgacgca ctgttggcga aaatcctcgg agaacggatt cagtgagcac atctggcgag
    58501 tcaccataaa gggaataaat tcgacaatgt tgcgatagtt ataaccgcta tctttgattt
    58561 tattggcaaa cgcggtgata tctgtggctt gttgcggggt aagatccggg actcgtaaga
    58621 cgaaaagctt atcactatgc ttcatcgctt ttttaaggga aacaatctgg acgccagcgc
    58681 ctgtcgcttc tgcaacgtta ttatcaccca gaaagattgc aacgtgactc acagaggaag
    58741 tgctgaagac gcggattcca aatgaggtta ccccaaggct tgaggagaac agcaaatcgc
    58801 cgggtttgag atcaggtgcc gttatttctt ttattgattg ttcggtgaaa gagctttgat
    58861 gctggaattt gacagcccat gtttttgcct cggcatctac tgctgtggct gatgggtctg
    58921 gctggctgat atcaacggta caagccgaaa gtaaaagaaa gctggggaga agcaggcggc
    58981 agtacgcctt tggtttatcc attttataca atccatgtaa aaaagggccc tgaaattcag
    59041 gaccctttct ggcatcagcc tttaatctgt ttcaccagat attcgacgat gtcaccagtc
    59101 ttaattaact gtttctcgcc gttgcgacga tatttatatt cgatatcgtc gttgtcgagg
    59161 ttacggtcgc ccagcacaat agtgtgcgga ataccgatca gttccatatc agcaaacatc
    59221 acgcccggac gctctttgcg gtcatccagc agcacttcga taccttgtgc acgcagttcg
    59281 ctgtacagtt tctcagcaag ctcttgtacg cggaaggatt tgtgcatgtt catcggcaga
    59341 atcgccacct ggaacggcgc gatagcgtca ggccatacga tgccgcgttc gtcgtagttc
    59401 tgctcaatcg ccgcagctac cacacgcgtt accccgatac cgtagcaacc catcgtcagg
    59461 atttggttac ggccatcttc accctgtacg gaggctttca gtgcttcgga gtacttggta
    59521 cccagctgga agatgtgacc aacttcgata ccacgtttga tcagcagcgt accctggcca
    59581 tccgggcttg gatcgccagc caccacgtta cggatatctg caacttccgg ggtagcgaca
    59641 tcgcgatccc agttgatacc gaagtagtgt ttaccatcga tgttagcacc agcagcgaaa
    59701 tcactcatcg ccgcaacggt acggtcaatc accaccggaa tcggcatgtt taccggaccc
    59761 agtgaacccg gaccggcttt aaccacagca cgaatttctt cttcggtcgc gaaagtcagc
    59821 gggcttgcaa cctgcggcag tttttctgct ttaacttcgt tcagctcgtg atcaccgcgc
    59881 accagcagcg caaccagcgg gaagctactg ccttcaaccg ctttaaccag cagagtctta
    59941 accgttttct caatcggcag attgaactgt tcaaccagtt ccgcgatggt tttcgcgttc
    60001 ggcgtatcaa ccagcgtcat ttcctgggta gcagcagcgc gcggttcttt cggcgcgata
    60061 gcttctgcca gttcaatgtt cgctgcatag tcagaggtgt cggagaagac cacatcgtct
    60121 tcaccgctct gcgccagcac ctggaattcg tgagaggcgc tgccgccgat agaaccggtg
    60181 tcggcctgta cggcgcggaa atccagcccc atgcggctga agattttgct gtaggccgca
    60241 tacattgcat cgtaggtttc ctgcagggat tcctgagaag tatggaaaga gtaagcatct
    60301 ttcatcagga attcgcggga acgcatgacg ccgaaacgcg gacgcacttc gtcgcggaac
    60361 ttggtctgga tctgatagaa gttcagcggc agctgtttgt aagagctaag ctcgttacga
    60421 atcaggtcag tgataacttc ttcatgagtt gggccgagta cgaacggacg ctcgccacgg
    60481 tcaacaaaac gcagcagttc cggaccgtac tgttcccaac gaccactctc ttgccacaaa
    60541 tcggctggct gaaccaccgg catcgacacc tcgatcgcac cggcgttgtt catctcttca
    60601 cgcacgatgt tttcgacttt tttcagaacg cgcacgccgg tcggcagcca ggtatataac
    60661 ccggaggcca gcttgcggat catcccggcg cgcagcatca gctgatggct gatcacctcg
    60721 gcgtcggcag gtgtctcctt gagagtggag agcaggtatt ggctagtacg catgttgtta
    60781 cggttccagt tggaaggtag aacaggctca aggcgagcct gggacaaaaa aagtgattta
    60841 gtttaccagt gcaaaagaaa tgtcaaaaga gaagggcgtg aatttaacgc ggttccagcg
    60901 caaagacttc aaaacctgcg tcggtgacgc gccagcgaac gttaaaatca tgtagccaga
    60961 cggcataggt tttgcccgtt tcctcacctt tacgataggc cgggcgcggg tcctgcgcca
    61021 gtacttcgcg gataaacagc gttaactgcg gataacgctt ctccagcgtc aaaagctgct
    61081 tttcgacctc tgcggtaaaa ctcaccgcca tctctgcagc tggcgcactt tgcgcatagc
    61141 tggcactggc atcgggaagc gattcggcaa aggggagata cggtttgata tccactaccg
    61201 gcgtaccatc gaccagatcc agactgccga gcttcagaat cacgctgtct ttatggcaaa
    61261 caacctcttt cagctctacc agcgacatgc caattgggtt agggcggaaa gtagagcgtg
    61321 ttgcgaaaac ccccattctg gcgttaccgc cgaggcgcgg tggacgcaca gtcggacgcc
    61381 agccgccttc catcgtttga tgaaagacga aaaggatcca taaatggctg aatgcttcca
    61441 ggccgcgaac ggcgtcggcc tggttgtagg aagcaatgag atgcagttcg ccgttggcgc
    61501 tttttaccag acctggctgg cgcggaacgg cgaatttttc tttatagggc gagcgaataa
    61561 cgcctatttg ctcgaactgg aaactgctca tttcgccgta atgttaagcg cagagccgat
    61621 acatacagcc tgacgatagc agcccggcgt accgctggtg acttcgcagc tatgcagtaa
    61681 tacagcattg gctttcattt tagaggcatt gatttgcatc tgcttacgtg cggttggaat
    61741 gctcggcgga gagtcctgat tagaggcctg gcaagagtcg ccactgactt caccgagatc
    61801 gcggaacggt ttgccgacta attcttctgc attggtataa attcggaccg gcgtggcgcg
    61861 cggcgctttc ggttttgcag gctccgcttt cggctggggt gcagtgcttt gaacgggttc
    61921 gacaggggat ctgcttaaca tggaacagcc gcttagcatg agtgctacta aacagatcgg
    61981 taaagcacgc atagtatttc ctcaatgtat gatcaaaacg tcaatattga atcaggagct
    62041 tgtaaaaatg acaagacggg caagcgcccg tcctgaatga tattacaaat tgtggaaaca
    62101 gcctaaaaat taccagcctt taacagcgcc gccgttaaac actttgtttg ctgcttcgta
    62161 aacttcatca gactgataag cctggacgaa tttcttcacg ttctcggcgt ctttgttatc
    62221 ttcacgcgtt acgatcaggt ttacgtatgg ggagtcttta tcttcgacaa agataccgtc
    62281 tttcgccgga gtcaggccaa tctggctggc ataggtggta ttgataactg ccagagcgat
    62341 ttgcgcgtcg tccagagaac gcggcagttg cggtgcttcc agttcaacaa ttttcagatt
    62401 ttttgggttc tcaacaacat caagaactgt cggcagcagg ccaacgccat ctttcagttt
    62461 gatcaagccc actttttgca gcagcagcag tgaacgacca agattagttg gatcgtttgg
    62521 cacggcaacc tgcgaaccat cctgcagttc atccagtgat ttgattttct tggagtaacc
    62581 agcaatcgga taaacaaaag tgttgcctac tgcgaccagt ttgtagccac gatctttcag
    62641 ttgctgatca aggtacggtt tatgctggaa ggcgttggcg tcgatatcgc ctttgctcaa
    62701 tgcttcgttt ggcagaacat agtcgttgaa ggttaccagc tcaacgtcca ggccatattt
    62761 gtctttcgca actttctgcg cgacttctgc tacctgctgt tcggcaccaa caatcacgcc
    62821 gactttaatg tggtttggat ctttttcatc ctgaccgcag cctaccagtg ccagtgatcc
    62881 aatcagggct cccactgccg caaaggtttt gaatttgaac gccatacctt attccttctt
    62941 caattattta tgttgtgttg aacgttactt gcgagtgaca gcccggacga tgcggtcgcc
    63001 tgcgaactga attaaataaa ccagaatgac cagcaatacc agtaccgtat tcatcaccgt
    63061 cgcgttgtag ccgatgtagc catactgata gccaatctga cctaaaccac cggcaccgac
    63121 tgcaccaccc atcgcggaat aaccgaccag ggtaatcagg gtgatagttg ccgcattcac
    63181 cagacccggc aacgcttccg gtaacagcac tttacggacg atctgcattg gcgtggcacc
    63241 cattgcgcgg gaagcttcaa ttaacccggt tgggatctcc agcagagcgt tctcgaccat
    63301 acgggcaata aacggtgctg caccaacggt taacggaaca attgctgcct gcaaaccaat
    63361 cgatgtaccg acaataacgc gggtaaacgg aatcatccat accagcaaga taatgaacgg
    63421 gatggaacgg aaaatgttca caatcgcaga aacggtacga tacagcttcg cgttagcaat
    63481 aatttgcccc ggacgcgtga cataaagcag aacgccaacc ggcagaccaa tcacaaaccc
    63541 aaaaaatccg gatacgaagg tcattgccag cgtttcccat acgccacgaa ccagcagcca
    63601 catcatcggc tcagacataa cccagtacct ctacttttac atggtgttcc tgcagccagg
    63661 caatggcggc ttgcgtatct tgttgtgtgc cgtgcatttc agtcagcatg atgccgaatt
    63721 taacgccacc ggcgtagtcc atctgcgcgc taataatgtt gttgttgacg ttgaaacgac
    63781 gcgcggtttc agaaagcagt ggggcatcga ccgattgacc ggtaaactcc agacgcagca
    63841 tcggcacgca gtcagtaaat ggctccgttt gcagacgttc ctggtaatct tccgggatat
    63901 ccagatgcag ggtcgactga ataaacttct gcgccagcgg cgttttcgga tgcgagaaca
    63961 cttcacttac cgtgtcctgc tcgatcagtt cgccattgct gatgaccgcc acgcaatcac
    64021 aaatacgctt cacaacgtcc atttcgtggg tgatcaacag aatcgtcaaa cccagacggc
    64081 ggttaatgtc tttcagcagt tcgagaatag aacgtgtcgt tgccgggtcc agcgcgctgg
    64141 tggcttcatc acacagcaat actttgggat tgctggctaa tgcacgggca atcgccacac
    64201 gttgtttctg cccaccggaa agattcgacg ggtagctatc atgcttatcg ccaagaccaa
    64261 ccaatgacag caattccgtc acgcgacgtt tgatctcgtc tttcggtgtg ttgtccagct
    64321 ccagcggcag agccacgttg ccaaaaacag tacgcgaaga gagcaggtta aaatgctgga
    64381 aaatcatacc aatctggcgg cgagctttgg tcaactcgga ttctgacagc gtggtcaatt
    64441 cctggccatc gaccagcacg ctaccctcgg ttgggcgctc cagcaggttt acacaacgta
    64501 taagcgtact cttacccgcg cctgaggcac cgataacgcc ataaatttgt ccagctggca
    64561 catgcaggct gacgttgttc aacgcctgga tggtgcgggt gccctggtgg aacactttgg
    64621 tgatattcga aagttttatc attgattatt tattatcgtc attaagttag tcgtagcatc
    64681 tcgaatgcct gaaacgggca acgccgtcaa tgaaatggat gttaaggcat ccagacgtct
    64741 aaatcaatca ggtttatgcg aagagcactt tcttgcaggt cgaaacatgc gatactagcg
    64801 tcccatgcct tattaaggag ctataaaagg tggcgaagag cgtacccgca atttttcttg
    64861 accgtgatgg caccattaat gtcgatcacg gctatgtcca tgagatcgac aactttgaat
    64921 ttatcgacgg tgttattgac gccatgcgcg agctaaaaaa aatgggcttt gcgctggtgg
    64981 tagtaaccaa ccagtctggc attgctcgcg gtaaatttac cgaagcacag tttgaaacgc
    65041 tgaccgagtg gatggactgg tcgctggcgg accgagatgt cgatctggat ggtatctatt
    65101 attgcccgca tcatccgcag gggagtgttg aagagtttcg ccaggtctgc gattgccgca
    65161 aaccacatcc ggggatgctt ttgtcagcac gcgattattt gcatattgat atggccgctt
    65221 cttatatggt gggcgataaa ttagaagata tgcaggcagc ggttgcggcg aacgtgggaa
    65281 caaaagtgct ggtgcgtacg ggtaaaccta ttacacctga agcagaaaac gcggcagatt
    65341 gggtgttaaa tagcctggca gacctgccgc aagcgataaa aaagcagcaa aaaccggcgc
    65401 aatgattaaa agatgaacgg ttgaaataaa aatgcatttt tccgcttgtc ttcctgagcc
    65461 gactccctat aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc
    65521 ggttcggttg aagagaaaaa atcctgaaat tcaggggtga ctctgaaaga ggaaagtgtg
    65581 atatacgcca cctcgcgaca gtgagctgaa agctgcgtcg caactgctct ttaacaattt
    65641 atcagacaat ctgtgtgggc actcgaagat acggattctt aacgtcgcaa gacgaaaaa
//
LOCUS       DAJTYF010000009        65066 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTYF010000009 DAJTYF010000000
VERSION     DAJTYF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.009"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            132..575
                     /locus_tag="OO590_000670"
     CDS             132..575
                     /locus_tag="OO590_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6636152.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /gene="ybhA"
                     /locus_tag="OO590_000671"
                     /pseudo
     CDS             811..1630
                     /gene="ybhA"
                     /locus_tag="OO590_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OO590_000672"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OO590_000672"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6636153.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTCIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OO590_000673"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OO590_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6636154.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OO590_000674"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OO590_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6636155.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OO590_000675"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OO590_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6636156.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="OO590_000676"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="OO590_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6636157.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="OO590_000677"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="OO590_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6636158.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="OO590_000678"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="OO590_000678"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6636159.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="OO590_000679"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="OO590_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6636160.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="OO590_000680"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="OO590_000680"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6636161.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="OO590_000681"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="OO590_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6636162.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="OO590_000682"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="OO590_000682"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6636163.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OO590_000683"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OO590_000683"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6636164.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13936..14316
                     /locus_tag="OO590_000684"
     CDS             13936..14316
                     /locus_tag="OO590_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6636165.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="OO590_000685"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="OO590_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6636166.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OO590_000686"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OO590_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6636167.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OO590_000687"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OO590_000687"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6636168.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
     gene            complement(17417..17492)
                     /locus_tag="OO590_000688"
     tRNA            complement(17417..17492)
                     /locus_tag="OO590_000688"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="OO590_000689"
     tRNA            complement(17496..17571)
                     /locus_tag="OO590_000689"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="OO590_000690"
     tRNA            complement(17623..17698)
                     /locus_tag="OO590_000690"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="OO590_000691"
     tRNA            complement(17701..17776)
                     /locus_tag="OO590_000691"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17913..17988)
                     /locus_tag="OO590_000692"
     tRNA            complement(17913..17988)
                     /locus_tag="OO590_000692"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17953..17955),aa:Lys,seq:ttt)
     gene            complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="OO590_000693"
     CDS             complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="OO590_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6636169.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18954..19475)
                     /gene="pal"
                     /locus_tag="OO590_000694"
     CDS             complement(18954..19475)
                     /gene="pal"
                     /locus_tag="OO590_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6636170.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="OO590_000695"
     CDS             complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="OO590_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6636171.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20935..22191)
                     /gene="tolA"
                     /locus_tag="OO590_000696"
     CDS             complement(20935..22191)
                     /gene="tolA"
                     /locus_tag="OO590_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6636172.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAK
                     AAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADI
                     NNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAA
                     KLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22256..22684)
                     /gene="tolR"
                     /locus_tag="OO590_000697"
     CDS             complement(22256..22684)
                     /gene="tolR"
                     /locus_tag="OO590_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6636173.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22688..23380)
                     /gene="tolQ"
                     /locus_tag="OO590_000698"
     CDS             complement(22688..23380)
                     /gene="tolQ"
                     /locus_tag="OO590_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6636174.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23377..23781)
                     /gene="ybgC"
                     /locus_tag="OO590_000699"
     CDS             complement(23377..23781)
                     /gene="ybgC"
                     /locus_tag="OO590_000699"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6636175.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23931..24224)
                     /gene="ybgE"
                     /locus_tag="OO590_000700"
     CDS             complement(23931..24224)
                     /gene="ybgE"
                     /locus_tag="OO590_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6636176.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24224..24337)
                     /gene="cydX"
                     /locus_tag="OO590_000701"
     CDS             complement(24224..24337)
                     /gene="cydX"
                     /locus_tag="OO590_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6636177.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24352..25491)
                     /gene="cydB"
                     /locus_tag="OO590_000702"
     CDS             complement(24352..25491)
                     /gene="cydB"
                     /locus_tag="OO590_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6636178.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25507..27075)
                     /gene="cydA"
                     /locus_tag="OO590_000703"
     CDS             complement(25507..27075)
                     /gene="cydA"
                     /locus_tag="OO590_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6636179.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27924..30557)
                     /gene="mngB"
                     /locus_tag="OO590_000704"
     CDS             complement(27924..30557)
                     /gene="mngB"
                     /locus_tag="OO590_000704"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR6636180.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKIWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30575..32491)
                     /gene="mngA"
                     /locus_tag="OO590_000705"
     CDS             complement(30575..32491)
                     /gene="mngA"
                     /locus_tag="OO590_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6636181.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWESVDGPEAIDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTVQQDT
                     QPVKSVKTELKQALLSGISFSVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMQ"
     gene            32661..33383
                     /gene="mngR"
                     /locus_tag="OO590_000706"
     CDS             32661..33383
                     /gene="mngR"
                     /locus_tag="OO590_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6636182.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33487..34356)
                     /gene="sucD"
                     /locus_tag="OO590_000707"
     CDS             complement(33487..34356)
                     /gene="sucD"
                     /locus_tag="OO590_000707"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6636183.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34356..35522)
                     /gene="sucC"
                     /locus_tag="OO590_000708"
     CDS             complement(34356..35522)
                     /gene="sucC"
                     /locus_tag="OO590_000708"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6636184.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35616..36833)
                     /gene="odhB"
                     /locus_tag="OO590_000709"
     CDS             complement(35616..36833)
                     /gene="odhB"
                     /locus_tag="OO590_000709"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6636185.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36848..39649)
                     /gene="sucA"
                     /locus_tag="OO590_000710"
     CDS             complement(36848..39649)
                     /gene="sucA"
                     /locus_tag="OO590_000710"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6636186.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39950..40666)
                     /gene="sdhB"
                     /locus_tag="OO590_000711"
     CDS             complement(39950..40666)
                     /gene="sdhB"
                     /locus_tag="OO590_000711"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6636187.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40682..42448)
                     /gene="sdhA"
                     /locus_tag="OO590_000712"
     CDS             complement(40682..42448)
                     /gene="sdhA"
                     /locus_tag="OO590_000712"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6636188.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42448..42795)
                     /gene="sdhD"
                     /locus_tag="OO590_000713"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42448..42795)
                     /gene="sdhD"
                     /locus_tag="OO590_000713"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6636189.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42789..43193)
                     /gene="sdhC"
                     /locus_tag="OO590_000714"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42789..43193)
                     /gene="sdhC"
                     /locus_tag="OO590_000714"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6636190.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43574..43681
                     /locus_tag="OO590_000715"
     CDS             43574..43681
                     /locus_tag="OO590_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636191.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43887..45170
                     /gene="gltA"
                     /locus_tag="OO590_000716"
     CDS             43887..45170
                     /gene="gltA"
                     /locus_tag="OO590_000716"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6636192.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45561..46127
                     /locus_tag="OO590_000717"
     CDS             45561..46127
                     /locus_tag="OO590_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6636193.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATCVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46178..48637
                     /locus_tag="OO590_000718"
     CDS             46178..48637
                     /locus_tag="OO590_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6636194.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALALEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGQVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48652..49383
                     /locus_tag="OO590_000719"
     CDS             48652..49383
                     /locus_tag="OO590_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6636195.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49380..50442
                     /locus_tag="OO590_000720"
                     /pseudo
     CDS             49380..50442
                     /locus_tag="OO590_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50594..51640
                     /locus_tag="OO590_000721"
     CDS             50594..51640
                     /locus_tag="OO590_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6636196.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51637..52428)
                     /gene="nei"
                     /locus_tag="OO590_000722"
     CDS             complement(51637..52428)
                     /gene="nei"
                     /locus_tag="OO590_000722"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6636197.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52464..53198)
                     /gene="pxpA"
                     /locus_tag="OO590_000723"
     CDS             complement(52464..53198)
                     /gene="pxpA"
                     /locus_tag="OO590_000723"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6636198.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53188..54120)
                     /gene="pxpC"
                     /locus_tag="OO590_000724"
     CDS             complement(53188..54120)
                     /gene="pxpC"
                     /locus_tag="OO590_000724"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR6636199.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(54114..54770)
                     /gene="pxpB"
                     /locus_tag="OO590_000725"
     CDS             complement(54114..54770)
                     /gene="pxpB"
                     /locus_tag="OO590_000725"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6636200.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQQHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54793..55536)
                     /gene="ybgI"
                     /locus_tag="OO590_000726"
     CDS             complement(54793..55536)
                     /gene="ybgI"
                     /locus_tag="OO590_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6636201.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55807..57288
                     /gene="dtpD"
                     /locus_tag="OO590_000727"
     CDS             55807..57288
                     /gene="dtpD"
                     /locus_tag="OO590_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6636202.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57438..58856)
                     /gene="phrB"
                     /locus_tag="OO590_000728"
     CDS             complement(57438..58856)
                     /gene="phrB"
                     /locus_tag="OO590_000728"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6636203.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYETARKGK"
     gene            complement(58853..59362)
                     /locus_tag="OO590_000729"
     CDS             complement(58853..59362)
                     /locus_tag="OO590_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6636204.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59491..59808
                     /locus_tag="OO590_000730"
     CDS             59491..59808
                     /locus_tag="OO590_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR6636205.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(59863..60243)
                     /locus_tag="OO590_000731"
     CDS             complement(59863..60243)
                     /locus_tag="OO590_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636206.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     EEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYTL"
     gene            complement(60246..64910)
                     /locus_tag="OO590_000732"
     CDS             complement(60246..64910)
                     /locus_tag="OO590_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6636207.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGGTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAVTDPLQQNTRYQYDHHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAQSHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDAMGRRTAF
                     GYDAYGRLVTLTNENGERYWFRHDVLDRLAEQINPDGCCQSYRYNALNAVTEVVFTGN
                     RGGEIRHRLARDAAGRLTAKETADGRTEYVHDAADQLLEIRRQRHETDAPEVIRFSYD
                     RLGRMLSEETAQGILTHQYDELGNRTATTFPDGRTQRHLYYGSGHLQQINLDREVISE
                     FTRDHLHREVLRSQGRLSTRQLYDPGGRLKRRETYSGMRGVVPETFTDRQYSYSGEDE
                     LLKTRHSRRGETDYFYDPTGSITACRSEDEGYLASWQYDAAGNLLGRRAGERATAENS
                     VVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTP
                     CAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYL
                     TGNKTKAGSYHWDDIKDPSVTKPPYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPR
                     ET"
BASE COUNT        15717 a        17652 c        15937 g        15760 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 accacttgta atcaattgat tataaaggat taattcataa agagcattta cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgcag gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gatgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag atgacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgccctct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggcgga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tataccaacc gcatcgccaa tagtcgttat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattacagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagtatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcgccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatga gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgtcgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctaga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctttcctaca atttctaact gtaattcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca cgccaccatt cagatggaat
    15301 atcaaccttg tcatgggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 accgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggcca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgtttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgcaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcgc attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttacattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtggtgggtc
    17821 gtgcaggatg actcgacttc gtctcgccct tcggcgccgt tgctgatgca acgttatcct
    17881 tcacatttta ctaccgcttc caccttagcg attggtgggt cgtgcaggat tcgaacctgc
    17941 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccac tttttcgttg
    18001 ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt tcagacttga
    18061 gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg acacgaccag
    18121 aaataatgcg acttctggtc gtgttttatg cattacatcg cgttcagacg tttttgtgcc
    18181 tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac ggctttcgct
    18241 tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc atcagcagcc
    18301 tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc atcatctttt
    18361 ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag gtaagttgaa
    18421 tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg gcgggattta
    18481 tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg catcgccgct tttcaccggc
    18541 gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc cgcaccgctt
    18601 tgatcgctgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac
    18661 aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc ctgaatctga
    18721 ccacgcaggg aatcaatatc ggattgatta tcagaaagtt gttgctggag ttgggttaaa
    18781 agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc gaccgagcct
    18841 gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc tataccaacc
    18901 agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa
    18961 accagtaccg cacgacggtt tttggagtat gctgcttcgt catgacccag tactgcaggt
    19021 ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt accctgcagg
    19081 tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc cggagtacca
    19141 cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg caggaagttt
    19201 gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc cagatcgaag
    19261 taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc ttcggaagac
    19321 atgttgccgt tgccgccgtt cgcatccata ccagtgccag cacccagcat gccttcgctg
    19381 ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac aggcagagca
    19441 atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt actattcaat
    19501 taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat cagttgccgg
    19561 aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc ccatcccctg
    19621 agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt catccaggaa
    19681 cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg
    19741 cccaccattg gagctgacca ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg
    19801 gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat tcactttata
    19861 aacctgcgga cgaccggcct ggtcagaagt aaatgccagg ttctggctat ccgggaacca
    19921 ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac cagaagccaa
    19981 atccattacg tacaggttca gactaccggt tttcgacaag gcgaatgcca gtttgctgcc
    20041 gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct gacgtacagc
    20101 gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg tcacataagc
    20161 cagtttagaa ccgtctggtg accacgccgg agacatcagc ggctgcggtg aacggtgaac
    20221 gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga actgaccgcc
    20281 gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatgc cggtcagttt
    20341 ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct tgttcacttt
    20401 gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc
    20461 aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag cgtcaatgcc
    20521 cagtgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct gtggcagacg
    20581 agcgcgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa cgatgccgcc
    20641 aatatcttca ggtgccgcac caggccccgc ccactggaaa ggaacaacac caataggacg
    20701 accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa ctgatgccca
    20761 cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc ccttatctgg
    20821 accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca aaactaccag
    20881 aaccccgtgg caaccggtgc ctgatgttga ccgtccgaac agtcaacatc gcgattacgg
    20941 tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg gtggtttcgg
    21001 gatcttcgca agtttagctg ctgccaacgc agcctgacaa agtgcgggat cgccaccttc
    21061 aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg tacaggtttt
    21121 gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa tctgcccggc
    21181 atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac tcccggcagg
    21241 cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac cagagcttag
    21301 ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt tttcagctgc
    21361 tgcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg ctttcttatc
    21421 agctgctgcc ttatcagcag ccgctttttc tgctgctttt ttgtcggctg cagctttctc
    21481 tgctgccgct ttcttatcag ctgcagcctt atcagctgca gccttttcag cagccgcttt
    21541 cttctcagct tctgccttgg ctttttcagc agcttcagtt gccgctttct ttcttgcttc
    21601 agctgcagct gcttctgccg cttccgcttt cttcttcagt gccgcagcgg ctacctcggc
    21661 ttttttctgc gcttcggctg cggctttggc ggcttctgct tctgcttttt tcttcgcgtc
    21721 tgcagccgct ttcttcgcag cttcttccgc agctttagca tctgccgccg ctttcgctgc
    21781 cgccacttca gcttgcttct gctttaactc ggcctgtttt gcggcttctt cagcctgctt
    21841 tttctgctcc tgagccgcta accgctcttt ctcaagttgc ttcaggcgtt cctgttcagc
    21901 cgcttgtttc tcacgcagtt cttcagcagc ctgctgttcc ttcatcttgc gctgttcatc
    21961 agaacgcttc gcgcttgatt cctggctttg catgcgtttg tactgctcaa ctaccgcacc
    22021 tgaatcaacc atgacagcgt cgatggacga accaccgccg cctccggctg aagcttctat
    22081 attctcatcg aacgaactcc agatcagcgc cgcaaataag atgacatgca gcactgctga
    22141 aataattatc gcccgcttga gcttgtcgtt ttgttcggtt gcctttgaca ctctcggttt
    22201 ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa gggaaacgca gatgtttaga
    22261 taggctgcgt cattaaacca accgatttca cacccgcact atgtaacaag ttcagtgctt
    22321 taattatttc atcgtaaggc acatcttttg cgccaccgat cagaaagacc gttttcgggt
    22381 tggccttgaa acggctggac acttccgcca ccacctgctc tggtggtaaa cgctccaggc
    22441 gatctttctc aaccaccacg gtgtactgac caataccaga cacttcaaca atcactggcg
    22501 gattatcgtt actgctcacc gcctgtgatt cagtagcgtc tggcagatcg acctccacgc
    22561 tctgggtgat gatgggcgct gtcgccataa agatcagcaa cagcaccagc agtacgtcca
    22621 gcaacggtac aatgttgatt tcggacttga gatcgcgacg acctcgtcca cgcgctctgg
    22681 ccatggctta ccccttgttg ctctcgctaa cggtaaacgc ctggcggtgc agaatcgcgg
    22741 taaactcttc cataaagttg tcgtaattca gttccagttt gtttacgcgc tggttgagac
    22801 ggttataagc cattaccgcc ggaattgcgg cgaacagacc aattgccgtc gcaatcaacg
    22861 cttctgcgat accgggcgca accatttgca gtgttgcttg ttttaccgcc ccgagggcga
    22921 taaaggcgtg catgatcccc cagaccgtac caaacagacc aatatacggg ctgatggagc
    22981 caaccgtacc gaggaacgga atgtgcgttt ccagattttc aagttcacgg ttcatggaga
    23041 tacgcatagc acgcgacgcc ccttccacta cggcttccgg cgcatggcta ttggcacgat
    23101 gcaggcgcac aaactctttg aacccgctgt aaaagatttg ttccgaacca gtcagattat
    23161 cccgtttccc ctgactctct tgatagaggc gagagagttc gattccagac cagaatttat
    23221 cttcaaacgc ttcggcttca cgcgccgctg cgttaagaat acgggttcgc tggataataa
    23281 tggcccagga tgcgattgaa aaaccaatca aaatcaacat gataagttta accagaaggc
    23341 tagccttcag gaacaaatca aggatattca tgtcagtcac tgcttaaact ccgcgacaat
    23401 agacttggga agcgcacgag gcttcatttt gagtgggtca acgcaaacaa ccagaacctc
    23461 tgcttcattc agcaaagtat tctcggcgtt gacaatatgt tgcgtgaaaa ccaaagaggt
    23521 gccacgcatt gatgttattt cagtctgtat ttcgagcata tcgtcgagcc gcgcaggtgc
    23581 ataatattcc accgtcattt tacgtaccac aaaggcaacg cgttcagcca tcagcgcctg
    23641 ctgactgaag tgatgatgac gcagcatctc tgtgcgtgct ctttcataaa aagcgacgta
    23701 actggcgtgg tacaccacac caccggcatc ggtatcttca tagtagacgc gaaccggcca
    23761 tcgaaacagc gttgtattca ctttacatcc cggtaatgca acaaaagtta gagcttttaa
    23821 acgctgctac tatacgcgcg cgattgaggt ttgggaatga gggcaagtta agggagcgta
    23881 aaaatttatg ggcctagagc ggcccataat tttttaaaga gaattctgat ttaaaagaag
    23941 aagaaaatca gcccgacaat gagaacaata tcggcaagca atgggcaaaa aatcccttgc
    24001 caaagaacct tctgcggacg aaagcccacg ccgtgaatca caccagcaca gacggcccac
    24061 atcagcaata aaccatgcca gatttccagt tcactggtct tcgcggcaaa acgagatggg
    24121 tcccaaaaca tacatcctgc taacagaagc gccatcacga aggaaagcgc ccgcaggggg
    24181 cgcttgtcca ttaccgcata caaggtcgcg ataatcttac tcatcagatg tcttcttgac
    24241 cggctttgcc tgattcgacg tgttcaagcg ccagcgcggt gattacccca aacgaacagg
    24301 caagaagcgt tcccagaatc catgcgaaat accacatttt tagctcctta cttagtacag
    24361 agagtgggtg ttacgttcaa tatcttcttt ggtgatacga ccgaacattt tccagtaaca
    24421 ccaggcggtg tagagcagaa tgatcggtac cagaaccacc gcaacccagg tcatgacgtt
    24481 aagcgtcagc tggctggaag ttgcatccca cattgtcaga cttgcgttca tcatggtgct
    24541 ggacggcatc acaaacggga acattgcgat accggctgtc aggatgatgc aggccagcgt
    24601 cagggaggag aacacaaacg cccacgcggc tttatccata cgtgcagtca ggatggtcag
    24661 cagcggcaga accacaccca gtgccggaat agcccacaga attggcgtgt tgttgaagtt
    24721 aaccagccat gcgccagctt cacgaaccac ttctttattc agtgggttag aggctgcgta
    24781 atggtccatt gtcgatttca cgacataacc atcgataccg tacatcaccc atacgccagc
    24841 cagtgcgaaa cagaccagtg tcaccagcgc agccacctga gccgttgcac gggtacgcag
    24901 gtgcagttcg cccccggtac gcatttgcag ataggttgcg ccctgagtaa tgatcatccc
    24961 tacgctcacc acgcctgcca gcaggccgaa cgggttaagc aactggaaga agttaccggt
    25021 gtagtacaga cgcagatatt catcaacgtt gaacggtacg ccctgcaaca ggttaccgaa
    25081 cgctacacca attaccagcg gcggaacgaa gctaccaatg aagatgcccc agtcccacat
    25141 gttacgccag cgggtttctt caatcttgga gcggtagtca aaaccgaccg gacggaagaa
    25201 caaagacgcc agcacgagga tcatcgccac atagaagccg gagaacgcag cggcatagac
    25261 catcggccag gcagcaaaga gtgcgccgcc cgcggtgatc agccaaacct ggttaccgtc
    25321 ccagtgtggt gcaatggagt taatcataat tcgacgctcg gtgtcgttac gaccgaggaa
    25381 acgggtgagc atgcccaccc ccatgtcgaa accgtcagtg actgcaaaac caatcagcag
    25441 aacgccaacc agcagccacc agataaaacg caatacttca taatcgatca tttgacgact
    25501 cctgtcttag cgtgccggct gagtagtcgt ggaagactgc tcaaagtgat agcgaccggt
    25561 tttcaggctg cttgggccga ggcgtgcaaa cttgaacatt aagaacaatt ctgccaccag
    25621 gaacagggta tacaggccgc aaatcagcac cattgagaag atgagatcgc ctgcggtcag
    25681 tgacgaattc gccacagctg tcggcagcac ttcaccgata gcccacggtt ggcggccata
    25741 ttcagccacg aaccagcccg cttctacagc aatccacggc agcggaatac cgtacagcgc
    25801 ggcgcgcaga agccattttt tctcgccaat gcggttgcgg atgacactcc agaaagagag
    25861 cgcgatgatt gccagcagca ggaagccaca cgccaccatg atacggaacg caaagtacag
    25921 cggcgctaca cgcgggatgg agtctttggt tgcctgttga atctgcgctt cagtcgcatc
    25981 agccacgttt ggcgtatagc gtttcagcag cagaccgtaa ccgaggtctt tcttcatgct
    26041 attgaactgg tcacgaaccg cctggtcggt agaaccagaa cgcagttgtt cgagcagaga
    26101 gtacgccttc atcccgttac gaatgcgttc ttcatgctgc accatcagct ctttcaggcc
    26161 gataaccggg gtatccacgg aacgcgttgc aatgatgccc agtgcgtaag ggatctgaat
    26221 cgcaaatttg ttcgtctcct cttcctgatc aggaatgccg aacaaagtaa aggcagcagg
    26281 cgcaggttgc gtttcccact cggcttcaat agcagccagt ttggttttct gcacgtcgcc
    26341 catttcgtag ccggattcat cacccagaac aataacagac agaacagcag ccataccgaa
    26401 gctggcagcg atagcaaagg agcgtttagc gaaggcgaag tcacgacctt tcagcatata
    26461 ccatgcgctg ataccgagga tgaacatcgc gccagtcaca taaccagacg ctacagtgtg
    26521 aacgaatttc acctgagcaa ccgggttaag caccagctcg gagaagctca ccatctccat
    26581 acgcatagtt tcaaagttga aatcggacgc gattgggttt tgcatccagc cgttcgcaat
    26641 cagaatccac agtgcggaca ggtttgaacc gagcgccacc agccaggtga cacacatatg
    26701 ctgaacttta cccagacgat cccaaccgaa gaagaacaga cctacaaagg tggattcgag
    26761 gaagaaggcc atcagacctt cgattgccag cggcgcaccg aagatatccc ctacatagtg
    26821 ggaatagtaa gaccagttag tcccgaactg gaactccatg gtcagaccgg tagccacacc
    26881 cagagcgaag ttgataccaa acaacttgcc ccagaacttg gtcatatctt tataaatctg
    26941 tttgccggag aggacgtaga ccgtttccat aatggccagg aggaacgcca taccgagcgt
    27001 cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca aactgtaagc gcgacagttc
    27061 gactatatct aacatcatga ctccttgctc atcgcatgaa gactccgaga gtgaaccccg
    27121 ttagaaaggg tcacacgcat gccccaatag aaatttgttt gaacactttc atcataccat
    27181 gtgaatgcac acaaaaacgg cgtaaagaaa ggttgcaaac atgttaataa aaactcaaat
    27241 tgatcccacg tatatattac gccgcaaaat ccttacaata aacaggtttt tgttgaagca
    27301 aatttgcatt tttcgacggt gatcaattta tagctaaatt gccgcctttc agccaatttg
    27361 atcgagaaca atttatctct ttttgatgcc catttccaag acttatacat tgataaatat
    27421 caatttctac aaattatgtt aatcatgttt caacaaggta actttagtga catttatgtt
    27481 taaaatgtgt gaattataag actgaaatta gtttcggttg gcaattaaag ataaaaagag
    27541 acgaagcaaa aacagcacgg ttgaccttaa ttcttttttt ggtcactggc taatacgacg
    27601 cagagtttca ggattattaa taaaggcata aatctcctcc ccgccgatat taacatttaa
    27661 ttaacttata attatattat aattaactca cccggggtta ttattttgtt aaaaataaag
    27721 ccacatatac gtagcttgag agtgaagata aacgcaaaat tacctgatgc gctacgcttg
    27781 ccagtgtcta catcatctct gcaatatatt gaatttgcct gcttttgtag accgaataaa
    27841 gcgttcatgc cgtatccggc atgaacaaag cgccctttgt caacaatctg gccgcgcata
    27901 tgtggcttcg gtttgattgc tattcaggca agccggtaac tgaacgtccg tgactggccg
    27961 ggtaaaaaag gccccgatag atttgaacct tgattttcct cggtggtaat gtgttcatcc
    28021 atcatcgttt ctgagcaaga aatcacctcg cgactgaaag caacagtcgc atcacaggtt
    28081 gctgattcag ccggattaaa cagccgtaaa atcacttctt gtcggtcttc agctttctta
    28141 agtgcgctta tcaggcatcc cactgggggc attttcaaca aactataact ttccggcacg
    28201 ttgaatccgg ctttgttgag cttcatcgca tcccatggga ttttgttgta acactgtact
    28261 ggagtcagcc atgctcgcgc ctgctgagct acaccagcgg cggttggcgt accggtataa
    28321 ctcaataaac ttaggcgaca agaaagcaga ccacgtagtt gtgagtccgg gactggcatt
    28381 ttaattcccg aaggccgcct aggccttaaa agcagatctt ctttccccag taagcccacg
    28441 ccgcgcagca acgtaatggc aaaggttttc ttctcttcac cgatgacttc aaattcacgt
    28501 agcccttcgc taaagacagc catgccgtta cgcccttcct gtaaggctgc atagttgagc
    28561 atattccata ccggaaccgg cgcttctttc cagccttctt gctgccagtt gttcattgca
    28621 ctgtcgttca cggggcgcgt tagcgaacca aactgggtat ctgccagaac actatcggtg
    28681 ttaaaagatg tagggatcag gacgcgaagg cgatgatcgt cagcctggtt atcaaggttg
    28741 atatccacat caatgcgcct gttgttatga ctaagagtga caaccaactc gacacctact
    28801 ctgccagtgg attgtcttgc gctgcgttgt tgcagattac gcggcaccgc catttcatag
    28861 cggataacag ccctgctctg ccaggcctca tgaataatct cgcattgcgg tttcgcattc
    28921 gccgaagtaa ttacccactc ttcttttgcg ggtgaatagt catattcatc accatcatca
    28981 gagctttctt caatttgcaa cacccggtca tagcgcacac cgctatcttt atctaccagt
    29041 tgtagcgttc catcctcatt gagcgcaatt tgccagaaag cattttccag tatcccttca
    29101 gcgtcacttt ttgccgcaat cacgttgcca ggctgattcg cttcgatata aagcgtgcga
    29161 tagcccatag aagggacaat ctggttgatc tgtatatcaa actccataaa gggatcgtaa
    29221 ttaccgtaat gaactatttg ccgatcgatt aggcctggat cgatctcacg ggcatggcga
    29281 ataaaatacg gtacaggctg accgcgatcg tctcgtagat taaactggct ggcgcgcagc
    29341 cgcacagtgg tgttgatcac ttcttcccgc ggccagggca tcaggttaaa caggacgagt
    29401 ttgtcggcgt cgctctgcgg catgttgtcg gcgattttac gcatatagaa acgcagcaga
    29461 ttatccgcca tgtcttcagc cagttcgaag cgggcgacga tttcgcgatg aactttgtca
    29521 ctacagcagc agccgatgct gtcgtgggca tgatttttta agatctcttt ccatattttc
    29581 tccagcaagc cgtggtggta ttcaaaaccc aacgtccagg ccagcgttgc cagcggttcc
    29641 agcagattaa caatcttatt ttcaatacgt gcgtgggcaa ctttgatatc catgcgcgtg
    29701 gaaccgatgg tgcgatgcac gcgcatatat ttgccatcaa taaattcccc tttcagggtt
    29761 gccagattat ctcgctgcgc ttcgatcttc tcaaatacct cttcaaagcg gctcatcaca
    29821 aactttcgtt gaggataaat ttcgcgcagt ttctccatca cttcgaagat attttgctgc
    29881 aatggcatct gatcatgccc attcggcagc aaaatctctt tggttacaga cgctttttcc
    29941 agcacgtcaa aataactgtc gaggcgtttg cggaatccgt tttcgtccgc aggtaagtac
    30001 ttaccgatgg cataacccaa cggcatcacc tgcgccgtca cttcgctacc gtcactgctt
    30061 tgccacaaaa actcggtttt atccgtaccg tggcgctcag aacatccacg ccagaacatg
    30121 gtgcaggtaa tgccaaatcc attgtagata tgcggcagtt gcccggacat gccaaaggaa
    30181 tcgggtaaat aacctatttt catcggctcg ccaaacgcga ggcagtcacg cattccgtac
    30241 atcagattac ggacgatgga ttccgcagaa acaatcgtgg tatcggtctg ggtataccag
    30301 gggccgataa tcaacttgcc ggcttctacc agtttcttca cgcggtcttt gttttccggt
    30361 ttcaccgcga aataatcttc gaggatcgcc gtttgcccgt cgagtacgta atatttgtat
    30421 tcgttgtcct gttccagtcg gcacaggatc tcttccatat tattgaccag cagaatgcgt
    30481 gactcttcgg tggtgaaata ccactcccga tcccagtgca tatgcggggt gatgtgaacg
    30541 cgagataccg ctttcatcgt cgtttccttt ttgtttattg cattacgcca tcagtcagat
    30601 agttgccatg cttaaccgcg tgacgccgcc agatcaggag aattgcagtc gagattgcag
    30661 cccccaccaa tgccgcacca aaccagccaa ttgccgccat aacaccgccc gcgccattat
    30721 catgaagtaa aaagagcgag aaaatgccag caccgggtgt cgaaaggccg atattcatcg
    30781 caccgacaat agcgcccgtt accatagagc ccagcacaaa cgaaccaata acccgcagcg
    30841 gatcttcaat cgccatcggg atcgcccctt cggtaatacc tgccagccct aacagccagg
    30901 tggatttccc ggtctcaatt tcaaactctt taaacaggcg cggtgcgagc atcgtggaag
    30961 cggttacggt aaatgccgaa accattttga cggaggcgaa aatggcatac gggccgtaaa
    31021 cgccgttcgc cattgcgccc aggcagaatg cataagcggc tttattcact ggccctccaa
    31081 ggtcaaagga acacataaaa ccgagaatgg cacccagcaa cagcgcgtta cttcctgaca
    31141 gaccgttcag ccaggcggta agcgagttat tgatccaggc gacaggttcc cccaccacaa
    31201 acagcatcag actgcccgct cccaatgtac cgagcaccag gtagagataa aaagtcagga
    31261 agccattgaa tttactgcta agacgcaagt gatttttcat ccagcgtatc aagtaaccgg
    31321 ctatcaatcc gccaacgacc gcgccgagaa acccggagcc gatcatgttg gcggcaagtc
    31381 cagccgcaaa gcctggcgct aacgccggtt tatctgccag cgaataggcg gtataggctg
    31441 ccagcaccgg taccatcaaa attccgagca gcccgccgcc cagtttgcgg tacatccaca
    31501 gccaggagtt ttcttcatta aacagatctt gtagcccgaa gatttgcgac agtaataccg
    31561 cgaccgccag caccgtcccc cccgcgacaa tcaacggtac ggaaaaagag atcccgctca
    31621 acagtgcctg tttcagctcc gttttgacgc ttttcaccgg ttgcgtatct tgctgtacgg
    31681 tacgcgtctc atcgccacgc tcgagggtaa gcgattgttg gatcaacgct tctgcatggc
    31741 gaatcggctc ggcaacaggc acagaaagcg caggaatgcc gttaaaacgc tcactctcct
    31801 tgatggcgac ttcagccgca aaaatacagg cagttgcact attgagttga tccgccgtta
    31861 aacgcccttc aataccgtta gcaccttgct tttcaacgta gacattcacg ccgagtttgc
    31921 gtccggcttt ttccagatac tccgcagcca tgtaggtgtg agcaataccc gccgggcagg
    31981 ccgttacgca aacgatagtt ggcgcgttgg aaaaagaggc agaaggttgc gtgcctccct
    32041 tgtcatccag cgccgagagc aattcatcag gcgtcgtcgc cgactgtata cgcgcccgaa
    32101 tctcatcatc cgccaggcgc gtggtcagcg ctgtcagcag ttgcatgtgc gtagtacccg
    32161 cttcattggg gggaatcgcc agcaacacca ctaaatcaat cgcttccggg ccatccacgc
    32221 tttcccactg aagcggctcg ctgagtgtgg cgaccgcaaa cgccgcttct tttaccgcag
    32281 cagttttgcc atgcggcaca gccaaccctt cacctaaggc ggtcgggcca aggctttcac
    32341 ggcgatacac ttctttcaga aattgctcag tactggaaat tttccccaga gcagcaagac
    32401 gttgagtcaa cgcgtgaatg gcctcttcac ggctggtaaa gcgcgcattc agacacaacg
    32461 catcgcggtg ggtcagagtc gtcaggttca tcatgatcct cacttgatcg tttttatagt
    32521 cgcgccagtg tgcccgataa aacaatacca ttgcgtaatc aatttcactt gattgaaatt
    32581 atttgcattg tatttgtatt atttacagga tgaatttatc gggcataatg gctaaattcg
    32641 tattaatgag atgctaaata atgggacaca aacccttata ccgacagatt gctgatcgca
    32701 ttcgtgagca aattgcgcgt ggcgagctta aacctggcga tgcgttacct accgaatcgg
    32761 cattacaaac agagtttggc gtcagccggg ttacggtgcg tcaggctttg cgccagttag
    32821 tcgagcaaca gatcctcgaa agcattcagg gcagcgggac ttacgtcaaa gaagagcggg
    32881 tcaattacga tatttttcag ttaaccagtt ttgatgaaaa actgtcggac cgtcacgtcg
    32941 atacacacag tgaagttctg atatttgaag tgattccggc tgacgatttt cttcagcaac
    33001 agctacaaat caccgtgcag gatcgtgtgt ggcatgtgaa gcgcgtgcgc tatcgcaagc
    33061 aaaagccaat ggcgctggaa gaaacctgga tgccgcttgc gttgttcccg gatctcacct
    33121 ggcaggtcat ggaaaattcg aaatatcact ttattgaaga agtgaagaag atggttatcg
    33181 atcgtagcga acaggaaatc attcctctga tgccaaccga agagatgagc cgtctgctta
    33241 atatcagcca gacaaagccg attcttgaaa aagtatcgcg tggatattta gttgatggtc
    33301 gggtgtttga atatagccgc aacgctttta ataccgatga ctataaattt accctgatag
    33361 cgcagcgaaa atcatcgcga taagcacaaa aaaggccatc atacgatggc caaccatgtc
    33421 gaaaccgaca gcccttcgcc attcctgtag agggaagcgg cgagggctat ttcttattac
    33481 agatatttat ttcagaacag ttttcagtgc ttcaccgata tccgccaggc tgcgaacggt
    33541 tttcacgcct gcggcttcca gagcagcgaa tttctcatcc gcagtccctt tcccaccggc
    33601 aatgatcgca cccgcgtggc ccatacgttt gcctttcggc gcagtcacac cagcgatgta
    33661 acccacaact ggcttggtaa catgctcttt gatgtacgct gccgcttctt cttcagcgct
    33721 accgccgatc tcaccgatca tcacgatcgc ttcggtctgc ggatcttttt cgaacatttc
    33781 gagaatgtcg ataaagttag agccagggat cgggtcaccg ccgataccga cacaggtcga
    33841 ctgaccgaaa ccgtaatccg tggtctgttt aaccgcttca taggtcagtg taccggaacg
    33901 ggaaacgata cccactttac ccggtttgtg aatgtgaccc ggctggatac cgattttgca
    33961 ttcacccggg gtgataacgc ctgggcagtt cgggccgatc atacgaacgc ctgcttcgtc
    34021 cagtttcact ttcacggtca gcatatccag cgtcgggatg ccttcagtga tggtgataat
    34081 cagtttgatg cctgcatcga tagcttccag aatggagtct ttgcagaacg gtgctggtac
    34141 gtaaataaca gacgcggaag cgccagtagc agcaacagct tcacgcacgg tgttgaacac
    34201 cggcaggccg aggtgggtag tgccgccttt acctggagtt acgccaccaa ccattttagt
    34261 gccgtatgca atggcctgtt ctgagtggaa agtcccctgg ctaccggtaa agccctggca
    34321 gataaccttg gtgtttttat caattaaaat ggacattatt tcccctccac tgcggcaaca
    34381 acctgctgag ctgcatccgt cagacctttt gctgcaataa tattcaggcc gctgtcagcc
    34441 agtttcttcg cgccgagttc ggcgttgtta ccttccagac gtaccacgac cggtacgtta
    34501 acacccactt ccgctaccgc gccgatgata ccgtcagcga tcaggtcgca acgaacgata
    34561 ccgccgaaga tgttaaccag aacggctttc actttgtcgt cagagaggat gattttgaac
    34621 gcttcggtta cacgttcttt ggttgcgccg ccgccaacgt ccaggaagtt agccggttcg
    34681 ccgccgtgca gtttaactat gtccatcgta cccatcgcca gacctgcgcc gttaaccata
    34741 caaccgatgt taccgtccag cgcaacgtag ttcagttccc actgtgcagc ctgtgcttca
    34801 cgcggatctt cctgcgactg gtcacgcatt tcgcgcagat caggctggcg gaacagggcg
    34861 ttgccatcag cgcccagttt gccgtcgagg caaatcagat cgccctgttt ggtgatgacc
    34921 agcgggttga tttcgatcag cgccaggtcg cgctccagga aaatggtcgc caggcccatg
    34981 aagattttgg tgaactgctg aaccagttta ccttccagac ccagtttgaa cgccagctcg
    35041 cgtccctgat acggcatcgg gccagtcagc ggatcaagcg caactttatg gatcaggtgc
    35101 ggagtttctt ccgccacttt ttcgatttcc acgccgcctt cggtggaggc cataaagacc
    35161 acgcgacggg aactacggtc tacaacggca ccgagataca gctctttagc gatatcggtc
    35221 gccgcttcaa ccagaatctg gttaaccggt tggccattgg catctgtttg atacgttacc
    35281 agacgcttgc ccagccagtt ttctgcaaaa gcacggatgt cttctttgct gtttacaact
    35341 ttcacaccgc ccgctttacc gcggccacca gcgtgaacct gacatttcac tacccacgga
    35401 ccggcaccga tttttgaagc ggcttcttct gcttcgcgcg gagtagtaca ggcataaccc
    35461 accggtgctg gtaagccata gcgggcaaac agttgttttg cctgatattc atgtaagttc
    35521 atgtgttctg tccatccttc agtaatcgtt atcttttaaa ccgtagaccg acaccctatc
    35581 cacgtcggtc tacagtgcag gtgaagctta aactactaca cgtccagcag cagacgcgtc
    35641 ggctcttcca gcaactcttt gatcgttacc aggaagccca cggattcgcg accatcgatc
    35701 agacggtgat cgtaggacag cgccaggtac atcatcggca ggatctcaac ctgaccattc
    35761 accgccatcg gacgatcttt gatagcgtgc atacccagaa ttgcgctctg cggcgggttg
    35821 atgatcggcg tagacatcag ggaaccgaac acaccaccgt tggtgatggt gaagttacca
    35881 ccggtcagat cttcaaccgt cagcttgcca tcacggcctt tgactgccag ctctttgatt
    35941 ttcttctcga tgtctgccat gccgagggta tcgacatcac gcagaaccgg cgtcaccagg
    36001 ccgcgcggcg tagaaaccgc catgctgacg tcgaaatagt tgtggtaaac cacgtcatcg
    36061 ccgtcgatag acgcgttcac ttccgggtaa cgtttcaggg cttcaaccac cgctttcacg
    36121 tagaaggaca taaagcccag acggatgccg tggcgttttt caaacgcttc accgtactgc
    36181 ttacgcagat ccataatcgg cttcatgttg acttcgttga acgtggtcag catggcggtg
    36241 gagtttttcg cttccagcag acgctctgcc acacgcttac gcaggcgagt catcgggaca
    36301 cgtttttcac tacgtgcagc cagagccggt tgcgccgccg gagcagccgc tgccggagca
    36361 gactctttcg ccggggcttt cgccagatgt ttttccacat cttcacgagt cagacgacca
    36421 cccacaccgg tgcctttaat ggcgctggcg tcgagattgt gttcagccag caggcgacgg
    36481 atcgccgggc ttaacgcatc gttgttttgc tcttccagag acgcctgctg gcgttgcgcc
    36541 ggagtggacg ctttctcttc agatttggcg ctggtttctt taccggcgct gttgccttca
    36601 cgcaggcgac caaggatctg acgagacgtt accgttgtac cttcatcttc cagaaccgca
    36661 tccagaatgc cgtctgctga tgccggtact tccagtacca ctttgtcagt ttcgatttct
    36721 accagcactt catcacgtac gactgcgtcg ccgggttttt tatgccaggt tgcgacggtg
    36781 gcatcggcta cggattcagg caggtcaggg accagaatat ctacgctact cattgtgtat
    36841 cctttattta ttcgacgttc agcgcgtcat taaccagatc ttgttgctgt ttctggtgaa
    36901 cggacatata ccctaccgcc ggagaggcgg aggccgggcg gcctgcataa cgcagagaag
    36961 ccccaaacgg aatcacttca cggaaatgat gctggctgca gtaccatgcg ccctggttga
    37021 gcggctcttc ctggcaccag acaaaatcct tgacgtgagc aaactgctgc aacacttcct
    37081 gcatcgcttt atgcgggaac gggtagagtt gctcgatacg cacaatggcg acatcgtgtt
    37141 gattgttctt acgacgctgt tccagcaggt cgtaataaac cttaccagaa cacatcacta
    37201 cgcgcttcac gcccttcgga tcaagttcgt cgatttcacc gatggctggc aggaaggtgc
    37261 cgttcgccag ttcttcgagg ctggaaaccg ccagcggatg acgcagcagg gatttcggcg
    37321 acatcacgac cagcggacga cgcatcccgc gcagcgcctg acgacgcagc atgtggtaaa
    37381 cctgtgccgg ggtagacggt acgcaaacct gcatgttttg ctcagcacaa agttgcagat
    37441 aacgttccag acgcgcggag gagtgctccg gcccctgccc ttcgtaaccg tgcggcagca
    37501 acatcaccag accacacatc cggccccatt tctgctcgcc agaggagatg aactggtcga
    37561 taaccacctg cgcaccgttg gcgaagtcac cgaactgcgc ttcccagatg gtcagggtgc
    37621 gtggttctgc ggtggcataa ccatattcaa acgccagcac ggcttcttct gacagtacgg
    37681 agtcccagac acggaacgcg ccctgaccgt tatggatgtg ttgcagcggc gtgtaagtgg
    37741 aaccgttaga ctggttgtgg atcaccgcgt ggcggtggaa gaaggtaccg cgaccggagt
    37801 cttcacccga caggcgaacc ggaatgcctt catcaaccag cgtggcgtaa gcgaggtttt
    37861 ccgcaccgcc ccagtcgaac agtttctcac cggctgccat cgcctggcga tcgccataaa
    37921 tcttggcaac gcgagactgc atttcaactg cttctggtac cgtactgata cgtttagcca
    37981 gttcctgcag gcgcttcatc tcaactttgt tcgggtactc ttcgtcccat tcgtggttga
    38041 ggtacggcga ccaggtgaaa gagtgcatgt tcatcggacg ccactctgcc actacgcaat
    38101 cgccagcatc cagcgcatcg cggtacaggt taaccatctc ggtggcatct tccagcgtcg
    38161 ccactttttc ctgctccagc ttgtcagcgt agattttgcg cggtgtcgga tgttttttga
    38221 ttttctgata catcagtggc tgggttgcgc tcggctcgtc ggcttcgttg tggccgtgac
    38281 ggcggtagca taccaggtcg atgaagacat cacgtttaaa ggtgttacgg aaatcgagcg
    38341 ccagacgggt cacaaaggca acggcttccg gatcatccgc gttaacgtgg aaaatcggtg
    38401 cctgaaccat cttaccgata tcagtacagt acggtgtaga acgggcatcc agcgggttag
    38461 aggtggtgaa accaacctgg ttgttgataa cgatacgtac cgtaccgcca acttcataac
    38521 cacgcgcttt cgacatgttc agagtttcct gaaccacgcc ctgcccggtc actgcggcgt
    38581 caccgtggat ggtgattggc agcactttgt tgctgctcgg ctcatcaagt ctgtccagac
    38641 gggcacgaac agagccgata acgaccgggc ttacaatctc aaggtgagac gggttaaacg
    38701 ccagcgccag gtgcaccagg ccgccatcgg tctggaagtc agacgagaag cccatgtggt
    38761 atttcacgtc gcctgtgccg aggtgttctt tatgtttacc ggcgaactcg tcgaacaagt
    38821 cttgcggttt tttacccagc acgttcacca gcacgttcag acgaccacgg tgcgccatcc
    38881 cgagaaccac ttcgcgggtg ccgctgttgc cagcgtggcg gatcatctct ttaagcatcg
    38941 ggattaacgc gtcaccgcct tccagcgaga agcgttttgc gccagggaat tttgcgccga
    39001 ggtaacgctc aaggccttca gcggcggtca gttcgcttaa gaagcgtttt ttctcttcgc
    39061 tattgaaagt cgcgcgacca gactcgatac gctgttggat ccagcgtttt tcttcggtgc
    39121 tggtgatgtg catatactcg gcaccaatcg ggccgcagta ggtttgcttg agggcggaaa
    39181 taagctcgcc gagtttcatg gtttctttgc cgctggcaaa tgaaccgacg ttgaaggttt
    39241 cctggaagtc tgcttcggtc agatcgtgga aagacggatc cagatcggcc actttatctt
    39301 gctgccacag tcccagcgga tctagattcg catgctggtg accacggaag cggtatgcgt
    39361 taatgagctg caggacttta acctgcttca cattggtgtc agggtcggag atcgttgaag
    39421 agtaacgtga agcgtctttc gccaggcggc ggaaatattc acgcgtttga gagtggaatt
    39481 gatccggttt gactcccgta ccaggtaact gctggaacgt cgaacgccag ttagcgtcaa
    39541 ccgagtcagg atcggttaag aagtcttcat agagctgttc tatccagctc tggtttgcgc
    39601 cagagaggta agaagagtcc aaccaggctt tcaaagcgct gttctgcatc gtgatccctt
    39661 aagcatcttt tttatgctta cttcgccgtg gatactacca cgcacagtga tgtgcgtgcc
    39721 ggggttcact tgcgagctct tgtctacgta gtggctcgcg aaggaacctt taaaaactgt
    39781 cttagtgtca ggggcagttt ttaaagattt cctgcatttc tttgttacgc ctgatgcgct
    39841 gcgcttatca ggcctgcggg taatgacttg taggccggat aaggcgttca cgccgcatcc
    39901 ggcactggtt gcctgatgca acgcttgcgc gtcttatcag gcctacagtt tacgcattac
    39961 gttgcaacaa catcgacttg atatggccga tggcgcgcgt cgggttcagc cccttcggac
    40021 atacactgac gcagttcatg atgctgtgac agcggaatac gctgaatgca tcgctcaaac
    40081 cgtcgaggcg gctgtcagtc tcggtatcac ggctatcgat caggaaacga tatgccgcta
    40141 acaagcctgc cgggccgata aacttatcgg gattccacca gaaagacgga caagaggttg
    40201 aacaacatgc gcagagaata cattcataca gcccgtcgag tttttcgcgc tgctctggca
    40261 tctgtaaatg ctcgcgagct ggcggatttt gtccattatt caacaggtaa ggcttaattt
    40321 tctcatattg cgcatagaat tgtcccatgt ctaccaccaa atcgcggatc accggtaaac
    40381 ctggcagcgg gcgaatcaca atcttcttgc ccggctggtt gagtgccgaa atcggggtaa
    40441 tacaggccag accattcttg ccgttcatgt tcagaccgtc ggaaccgcac acaccttcac
    40501 ggcaggagcg gcggaacgac aggctgggat ctttctcttt cagctggata agcgcatcca
    40561 gcagcatcat gtcgcgacct tcttccgctt ccagggtgta atcctgcata cgcggagcat
    40621 catcaacatc cgggttatag cgataaattg aaaactcgag tctcattttc ctgtctccgc
    40681 attagtaagt acgaatcttc ggcgggaatg ccgggcgcag tttcggttcc atgttgacgc
    40741 ttcggcgcgt catggattcc gactcaggca gatacaggga gtggcacagc cagttttcat
    40801 catcacgatc cgggaagtcg aagcggctat gtgcgccacg gctttcggta cggaagttgg
    40861 cagaaacagc cgttgcatac gccgtttcca tcaggttatc cagttccagg cactcaacgc
    40921 gctgggtgtt gaactcgctg gaagtgtcat ccagacgggc atttttcaga cgctcgcgga
    40981 tcactttcaa ctgctcaagc cctttcgcca tcgcatcacc ttcacggaag accgagaagt
    41041 tatgctgcat acattcttgc agcgctttac ggatcgccac cggatcttca ccgttacgat
    41101 tattgttcca ccggttcagg cgatccagag acgcttcaac atcagactcg ctggcatcgc
    41161 gcagtgcgcc ctgctcggcg atagactctt gcagatgcag acctgccgcg cgaccaaaga
    41221 ccaccaggtc cagcagcgag ttgccgccca gacggttagc gccgtgtacc gatacacaag
    41281 cgatttcacc aacggcaaac agtcccggaa caaccacatc ttcgcctttc tcattcacag
    41341 tcagcgcctg accggtaact ttggtcggaa taccgcccat catgtagtga caggttggga
    41401 taaccggaat cggctctttc accggatcga cgtgagcgaa ggtacgggaa agctccagga
    41461 tacccggcag acgggattcg agaacttctt tacccaggtg atcgagtttc agtttcgcgt
    41521 gtggccccca cggaccatca cagccgcgac cttcacggat ttcaatcatg atggaacgcg
    41581 ccaccacatc acggcccgcc aggtctttgg cgttcggcgc ataacgctcc ataaagcgtt
    41641 cgccatgttt gttcagcaga taaccgcctt caccacggca accttcggtg accagtacgc
    41701 ccgcaccggc aatgccggtc gggtggaatt gccacatttc catatcctgc accggtacgc
    41761 ctgcgcggat agccatgccg acaccgtcgc cagtgttaat gtgggcgtta gtggtggact
    41821 gataaatacg ccctgccccg ccagtcgcca gcacggtagc gcgggctttg aaataaacca
    41881 cttcaccggt ttcgatgcac agtgcggtac aacccaccac cgcgccatcc tggtttttca
    41941 ccagatccag cgcataccac tcggagaaaa tggtggtgtg gtttttcagg ttctgctgat
    42001 aaagcgtgtg caacagtgcg tgaccggtac ggtcagcagc cgccgcagtg cgtgccgcct
    42061 gctcgccgcc gaagtttttc gactgaccgc caaacggacg ttgatagata cgaccatcat
    42121 cgagacgcga gaacggcagg cccatgtgtt cgagttccag aatcgcttcc ggcccggttt
    42181 tacacatata ttcaatcgcg tcctggtcac cgatatagtc cgaccctttt acggtgtcgt
    42241 acatatgcca ttcccagtta tcttcatggg tattacccag cgcaacggta atgccgcctt
    42301 gcgcagaaac ggtatgggaa cgggtcggga agactttaga gagcagcgca caggtctggc
    42361 cgctctggga aatttgcagc gccgcgcgca tacctgcgcc accggcacca atcacaactg
    42421 catcaaattc tctgactggc aatttcatca caccccccac accacaacga atccataaat
    42481 cacgtaaacc accagtgcaa cgacaatcac cagttgcagc atcaggcgca aggccagcgg
    42541 tttaacgtag tcggtcaaca cctgccacat gccgatccag gcatgaatca agatagaaaa
    42601 cagcgccagc agggtgaaca ctttggtgaa cgcagaggcg aagaaaccga tccagacttc
    42661 atatgtcagt tcgccactgg tggcgaaaaa accgaccata taaatgatgt agagcgtcag
    42721 gacgatagcg gtagcgcgaa cgaggatgaa atcatgtacg ccattgcgtc ctaatgcgga
    42781 ggcgttgctt accatacgag gactcctgcg agaagtgaaa gcacgacagt aataacaaag
    42841 gagattttgg cggagcgttt acccgcttcg aatgtttctt ccagatagcc aaaatccatc
    42901 atcatgtggc gaatacctac gacgacgtga tacgccagag cggtaaggat gccccacatg
    42961 ataaatttga cgaagaagct gcccataatc gcggaagctt gctcgaaacc ttcaggggaa
    43021 gagaggctgg tacccagaag ccacagcagg atgcccactg caacaaaggt gatcacaccg
    43081 gaaacgcgat ggagaatgga cgctatcgcc gtgatgggga accggatggt ctgtaggtcc
    43141 agattaacag gtctttgttt tttcacattt cttatcatga ataacgccca catgctgttc
    43201 ttattattcc ctggggacta cgggcacaga ggttaacttt ctgttacctg gagacgtcgg
    43261 gatttccttc ctccggtctg cttgcgggtc agacagcgtc ctttccataa ctgcgcgtca
    43321 tgcaaaacac tgcttccaga tgcgaaaacg acacgttaca acgctgggtg gctcgggatt
    43381 gcagggtgtt ccggagacct ggcggcagta taggctgttc acaaaatcat tacaattaac
    43441 ctacatatag tttgtcgggt tttatcctga acagtgatcc aggtcacgat aacaacattt
    43501 atttaatttt taatcatcta atttgacaat cattcaacaa agttgttaca aacattacca
    43561 ggaaaagcat ataatgcgta aaagttatga agtcggtatt tcacctaaga ttaacttatg
    43621 taacagtgtg gaagtattga ccaattcatt cgggacagtt attagtggta gacaagttta
    43681 ataattcgga ttgctaagta cttgattcgc catttattcg tcatcaatgg attctttacc
    43741 tgcaagcgcc cagagctctg tacccaggtt ttcccctctt tcacagagcg gcgagccaaa
    43801 taacaaacgg gtaaagccag gttgatgtgc gaaggcaaat ttaagttccg gcagtcttac
    43861 gcaataaggc gctaaggaga ccttaaatgg ctgatacaaa agcaaaactc accctcaacg
    43921 gggacacagc tgttgaactg gatgtgctga aaggcacgct gggtcaagat gttattgata
    43981 tccgtactct cggttcaaaa ggtgtgttca cctttgaccc aggcttcact tcaaccgcat
    44041 cctgcgaatc taaaattact tttattgatg gtgatgaagg tattttgctg caccgcggtt
    44101 tcccgatcga tcagctggcg accgattcta actacctaga agtttgttac atcctgctga
    44161 atggtgaaaa accgactcag gaacagtatg acgaatttaa aactacggtg acccgtcata
    44221 ccatgatcca cgagcagatt acccgtctgt tccacgcttt ccgtcgcgac tcacatccaa
    44281 tggcagtcat gtgtggtatt accggcgcgc tggcggcgtt ctatcacgac tcgctggatg
    44341 ttaacaatcc tcgtcatcgt gaaattgccg cgttccgcct gctgtcgaaa atgccgacca
    44401 tggccgcgat gtgttacaag tattccattg gtcagccatt tgtttatccg cgcaacgatc
    44461 tctcctatgc cggtaacttc ctgaatatga tgttctccac gccgtgcgaa ccgtatgaag
    44521 ttaatccgat tctggaacgt gctatggacc gtattctgat cctgcacgct gaccatgaac
    44581 agaacgcctc tacctccacc gtgcgtaccg ctggctcttc gggtgcgaac ccgtttgcct
    44641 gtatcgcagc aggtattgct tcactgtggg gacctgcgca cggtggtgct aacgaagcgg
    44701 cgctgaaaat gctggaagaa attagctccg ttaaacacat tccggaattt gttcgtcgtg
    44761 cgaaagataa aaatgattct ttccgcctga tgggcttcgg tcaccgcgtg tacaaaaatt
    44821 acgacccgcg cgccaccgta atgcgtgaaa cctgccatga agttctgaaa gagctgggca
    44881 ccaaagatga cctgctggaa gtggctatgg agctggaaaa catcgcgctg aacgacccgt
    44941 actttatcga gaagaaactg tacccgaacg tcgatttcta ctctggtatc atcctgaaag
    45001 cgatgggtat tccgtcttcc atgttcaccg tcattttcgc aatggcacgt accgttggct
    45061 ggatcgccca ctggagcgaa atgcacagtt acggtatgaa gattgcccgt ccgcgtcagc
    45121 tgtatacagg atatgaaaaa cgcgacttta aaagcgatat caagcgttaa tggttgattg
    45181 ctaagttgta aatattttaa cccgccgttc aaatggcggg ttaattttta tatgcctaaa
    45241 cacaaaaaat tgtaaaaata aaatccacta tcggacattc atatattcta aaaaaaaata
    45301 tatcggtata aattataaac aaatcgatga tttttaatca tctatcttcc tctcagttac
    45361 atgaataaac caccaaatat agcgcacccc tataatttcc aatactttat tcatcaaaca
    45421 tttaattcat accatcaatt aattactttt acatttaata aaattaataa tatttttaag
    45481 aaaaactaaa aacattcgac aatcaaataa gatattcttg caccgatata cttttttctt
    45541 ttctgttaaa ggactcagtt atgtttaaag gacaaaaaac attggccgca ctggccgtat
    45601 ctctgctgtt cacagcacct gtttatgcgg ctgatgaagg atccggtgaa attcacttta
    45661 aaggtgaagt tattgaagca ccgtgtgaaa tacatcagga tgatattgat aaagaggttg
    45721 aactcggtca ggtgaccacc agccacatta atcagtcaca tcacagcgat gccgttgctg
    45781 tcgacctgct cttagtcaac tgtgatctgg aaaactccag caacggttcc ggtggcaaga
    45841 tttctaaagt tgcagtcacc tttgatagct cagcgaaaac caccggcgca gatccgattc
    45901 tcaacaacac cagcacgggt gaagccacct gcgtcggcgt acgtttaatg aataaagatc
    45961 aaagcaatat cgttttaggc accgctactc cagatatcga cctggctccg acctccagcg
    46021 aacaaacgct gaatttcttt gcctggatgg aacaaattga tcaggctaca cctgtaacgc
    46081 caggcgcagt tacggcgaat gcaacctacg tgctggatta taaataagta ttactttggc
    46141 ggcgctatcg ccgcctttca taaaatattt atcaggtatg gacaccgtga atatttatcg
    46201 actctctttt gtatcctgcc tggtcgtggc gatgccttgc gcattggcgc tcgaattcaa
    46261 tctgaatgtt ctcgataaat caatgcgcga ccgcattgat atttcattat taaaggaaaa
    46321 aggagtcatt gctcccggtg agtattttgt tagcgttgcg gtaaataata accaaatcag
    46381 taacgagcaa aagattaact ggcacaaaaa tgacgataaa accattccgt gcatcaatga
    46441 tttactggtc gataaatttg gcttaaaacc tgaagtccgt cagtcattac cattgataaa
    46501 tcagtgcgtc gattttagct cccgacctga aatgctcttc aatttcgatc aagccaatca
    46561 gcaactgaat attagtattc cgcaagcctg gctggcgtgg tactcagaaa actggacccc
    46621 accctccaca tggaaagaag gtgtcgccgg tgtcctgatg gattacaact tgtttgccag
    46681 cagctaccgc ccacaggatg gcagcagcag cactaatctt aacgcctatg gaacagccgg
    46741 aatcaacacg ggggcatggc gcttacgcag tgattatcag ttgaatcata ctgatagcga
    46801 tgataaccat gaacagtcag gcgaaatatc acgtacctat ctttttcgcc cgttaccgca
    46861 attaggttca aaattaaccc tcggcgaaac ggattttagt tccaatattt tcgacggttt
    46921 ttcttatacc ggcgcggcac tggcaagtga cgatcgaatg ttgccatggg aactacgcgg
    46981 ctacgcccca caaattagcg gtattgcaca gaccaatgcc acggtgacga tcagtcaatc
    47041 aggccgcgtc atttaccaga aaaaagtccc accaggccca tttatcattg acgaccttaa
    47101 tcagtctgtt cagggcacac tggatgtcaa agtgacggaa gaagatggtc gggtgaacaa
    47161 tttccaggtt tcagcagcat cgacgccctt cctgactcgt cagggacagg ttcgctataa
    47221 actggccgcg ggtcagccac ggccctccat gtcacatcaa actgaaaatg aaaccttttt
    47281 tagcaatgaa gtttcctggg ggatgctgtc aaacacctcg ctgtacagcg gcctgctgct
    47341 ttctggtgat gactaccatt ctgccgcaat gggtattggg caaaatatgc tgtggcttgg
    47401 tgcgctgtcg tttgatgtca cgtgggccag tagccatttt gatactcagc aggacgagcg
    47461 gggcttaagc taccgtttta attacagcaa acaagtggat gccactaaca gcacgatttc
    47521 cctcgccgct tatcgtttct ccgatcgtca ttttcacagc tacgccaact atctggatca
    47581 caaatacaac gacagcgatg cgcaggacga aaaacagacg atcagcttat ctgtgggcca
    47641 accgattacc ccactaaacc tcaatcttta cgccaacctg ctacatcaaa cctggtggaa
    47701 tgcagacgcc tccacgaccg ccaacatcac ggcaggtttt aatgttgata ttggtgactg
    47761 gagagatatc tctatttcta cgtcattcaa tacaacccat tacgaagata aagatcgcga
    47821 caaccagatt tacctgtcga tttcgctccc cttcggtaac ggtggtcagg ttggctatga
    47881 catgcaaaac agtagccaca gcaccacaca ccgcatgtcg tggaacgata cgctggatga
    47941 acgtaatagc tggggcatgt ctgccggact gcaatccgac cgtcctgaca atggagccca
    48001 ggtgagcggt aactatcagc acctgagttc agcgggtgag tgggatattt ctggtaccta
    48061 tgccgccaat gattacagtt ccgtcagcag cagctggagc ggttctttca ccgcaaccca
    48121 atatggtgca gcgtttcatc gccgcagctc caccaatgaa ccacgcctga tggtcagcac
    48181 cgatggcgtg gcagatattc cggttcaggg caatctcgac tacaccaacc attttggcat
    48241 tgcggtggtg ccgttgattt ccagttacca gccttccacc gtggcggtga acatgaatga
    48301 cttacccgac ggcgtaacag ttgcagaaaa cgttatcaaa gaaacgtgga ttgaaggcgc
    48361 gataggttac aaatcactgg cttcccgttc cggtaaagac gttaacgtca tcattcgcaa
    48421 cgccagcggt cagtttcctc ccctcggagc ggatatccgc caggatgaca gcggcattag
    48481 cgtggggatg gttggcgagg aaggacatgc ctggttaagc ggagtcgctg aaaatcaaaa
    48541 gtttaccgtg gtctggggtg atagccagca ttgctcgctc catcttcctg aacatatgga
    48601 agacaccgca aatcgcctga ttttaccttg tcattaatta aggaaatgaa aatgacattt
    48661 atgaaaggac tacccctttt gttgttggtt gccagtctgt gcagccacgc tgcactacaa
    48721 cccgatcgca ctcgtattgt gtttaacgct aatgataaag ctaccagtct gcgggtggat
    48781 aatcgtagcg ataaactccc ctacctcgct tattcctggc tcgaaaatga aaaaggggag
    48841 aaaagcgatg atcttctggt cgccctgccg ccgatacaac gtctggaacc gaaagcaaca
    48901 acgcaggtac ggatagtaaa gcaagcctcg accacaaaat taccgggcga tcgcgaaacg
    48961 ctatttttct acaatatgcg tgaaattcct cccgccccgg aaaaaaacag cgaccatgcc
    49021 gttctgcaag tcgccattca gagtcgtatc aaggtgttct ggcgaccggc tgcattacgc
    49081 aagaaagcag gagaaaaggt cgaactgcag ttacaggtca gccagcaggg caaccaactg
    49141 acactgaaaa atcctaccgc gtattatctg accattgcct acctgggacg aaatgaaaaa
    49201 ggcgttctcc ccggcttcaa aaccgtgatg gttgcgcctt ttagtacggt caacacaaac
    49261 accggaaatt atagcggcag ccagttttat ctcggttata tggatgatta cggcgcgtta
    49321 cgcatgacga cgctcaactg cagcggacaa tgccatttac aagccgtgga ggcgaagaaa
    49381 tgagtgctgg caagggagtg ttgctcgtca tctgtctgtt atttctgccg ttgaagtctg
    49441 cgcttgccct gaactgctac tttggttcat ccggtggctc ggtagaaaaa tcagaagcga
    49501 ttcaaccttt tgctgtacca ggcaatgcca aactcggcga taagatctgg gaatctgacg
    49561 atattaaaat tcccgtctat tgtgacaaca ataccaacgg caactttgaa agcgaacatg
    49621 tctacgcatg ggtaaacccc tacccggggg tgcaggatcg ttattatcaa ctcggagtaa
    49681 cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac actaaccagt
    49741 gtatcgacag taagaacatt gacatttata cccttgagca gatcatcgcg atgggatggc
    49801 agaacaaaat ttgctcgggt gatcccgcta atattcacat gtcgcgtacc tttctcgctc
    49861 gcatgcggtt atacgtcaaa atacgagaaa tgccgccgca tgattatcaa agtacgctta
    49921 gcgactatat cgtcgtgcaa tttgacggtg ccggtagcgt taacgaagac cctactgccc
    49981 aaaacctgaa atatcatatt actggtctgg aaaacattcg cgtgctggat tgcagcgtca
    50041 atttttccat ttcaccggaa acacaggtga ttgattttgg taaatttaat ttgctggata
    50101 ttcgtcgcca cacaatgtcg aaaacattca gtattaagac gacaaaatca caaaatgacc
    50161 aatgcaccga cggatttaaa gtcagttcct ccttttatac cgaagaaacg ttggttgaag
    50221 aagataaagc attgttgatt ggtaatggtc tgaagctacg tttgctggat gaaaacgcct
    50281 cgccttatac cttcaataaa tatgctgaat acgccgattt caccagcgac atgttggtct
    50341 acgaaaaaac ctatacagct gaactttcgt ccattgcagg tacccccatc gaggctgggg
    50401 cccttcgata cagtggtgct ttttaaaatt aactataact gatttgccat gatgcccttt
    50461 gcagccggaa agggcatctc tttcagaaaa ttgctgattt tccccttttc cttgattgct
    50521 gttatttttt cctagcccgg ctttctttat gctgtatcga tacaccaaag agaaaataat
    50581 gagggagcga aggatgccag ttttgcagtg gggaatgtta tgtgtgctat cacttctcct
    50641 ttctattggt ttcctcgcgg tacatctccc ggcggcgcta ttacttgggc cgatgatcgc
    50701 cgggatcatc ttcagtatgc gcggaattac tctgcaactt ccccgctccg cttttctcgc
    50761 cgcgcaggcc attcttggct gcatgattgc gcaaaacctc actggttcga ttctcaccac
    50821 gctggccgcc aactggccga tcgtgcttgc tattttactg gtgacgctgc tttccagcgc
    50881 catcgtgggt tggttattgg tgcgctatag ctcactgcct ggaaataccg gtgcctgggg
    50941 ttcttctcca ggcggcgcag cggcaatggt cgctatggcg caagattacg gcgcagatat
    51001 tcgcctggtg gcgtttatgc aatatctgcg agtgctgttc gtcgcgggtg ccgctgttct
    51061 ggtcacccgc atgatgctgg gtgataacgc tgaagcggtt aaccagcaaa tcgtctggtt
    51121 cccgccagtg agcattaatc tcctgcttac gattttgctc gcagtcgttg ccggtacggc
    51181 gggatgcatg ctgcgtcttc cttccggcac gatgctcatc ccgatgctgg cgggcgcagt
    51241 gctccagtct ggtcagctca tcaccatcga actaccggaa tggctgctgg cgatggcgta
    51301 tatggcaatt ggctggcgga ttggtcttgg tttcgataaa caaatcttac tgcgggcact
    51361 acgcccgcta ccgcaaatcc tgctgtcgat ttttgctctg ctggctattt gtgcaggtat
    51421 ggcgtgggga ctgacccggt ttatgcatat tgattttatg actgcctacc tcgccaccag
    51481 tcccggcggg cttgataccg tggcggtcat cgccgcaggg agcaatgccg atatggcgct
    51541 tattatggcg atgcaaaccc tgcgcctgtt cagtattttg ctgacagggc cagccattgc
    51601 gcggtttatt tcaacctatg cgccgaagcg gtcggcctag tgctggcagc ctggacacca
    51661 gtaaaacggg cgcgaggaca acgtggtttt ctcaatgatg ccgccacagc gttcgcacag
    51721 ttcgccatca cgatgaaaaa ccttaaagcg aaacagcgcc ccatgatgct tattctcatc
    51781 cacctgcccc cgcgtagcgt aagaaaaccg aggaatctcc agtaacgcgt gtgcgagtgc
    51841 atccagttgc gccgcattga gatctttcgc tttatgattt ccagtcaacc caacctgcca
    51901 gaggatctcc acccgcaaat aattgccaag cccagccaga aacgcctgat cgagcagtaa
    51961 tcctgcaaac tgacggttac gaaagcgcgg cgacaataac cgttccttta ccacctccgg
    52021 cgtcagattc ggatccagca catcgggacc aacgcgttgt aaaaacggat gcgtggtcag
    52081 ttgttccggc ctcaacatct caatatccga ggcgctataa agcagaatgg ttttgtcagc
    52141 cgtttgcagt tttacccgca atactcgcgt ggtctgcggc tcttcgccgg tatcaaccac
    52201 gcgccagacg ccgtaaagct gattatggct gtagagcgtt aaaccgttgg gaaaatgagt
    52261 taacaacgcc ttaccacgcg tttccacatg ggtaacgtgt tgaccgataa gttgtgattg
    52321 ataagttttt aactgcggga aggcaaacca gacatcagtt agtggtttgc ctttgatcgc
    52381 cgcctccagg ttatccgctg cacggcggat ctccgggcct tcaggcatgg tgatatcctt
    52441 ttttcattct ttattaagcg gggttatgct gcgacaacaa tccccttttt ggcaaatgta
    52501 gctcgtaatc ggcgggcgaa cgccagagcg tgctcgccgt cgccatgcag gcagaccgtt
    52561 tgcgccgcga ccgttgccca ttcgccggtg atacttttga ctctgccgtg ttgcaccatc
    52621 tccagcgttt gcgccaacgc ctgttcttcg ttttcaatca acgcgcctgg ctgacttcgc
    52681 ggcaccagcg agccgtcagc ctgataaccg cgatcggcaa acacttcctc gcgcgttgtc
    52741 aggccatatt gcttgcctgc acgaatcagc tcgcttcctg ccagcccaac gagaaccaat
    52801 gccggatcgc aagcgtatac cgctctggcg atggcgtctg ccagttgtgc ttctttcgcc
    52861 gcctggttgt acaacatgcc gtgcggtttg acatgacgca ttacgccgcc ttgcgcacgg
    52921 gcaatcgttg ccagcgcgcc aatttgatac agcgtctggg cgtaaacggt ttccggcggc
    52981 agctgcatgg cgctgcgacc aaaattttcc ctgtcgggaa aactcgggtg agcgccaatc
    53041 gcgacaccat tttttattgc ttcacgcacg caagcctgca tggtttgcgc atcgcctgca
    53101 tgaaatccac aagcaatatt ggcagaggaa accagcgtta atagctctgc gtcgctggcg
    53161 cagccttcgc ccagatcggc gttcaggtca attttcattg tgcagccgcc acgctaattg
    53221 ttcaaaataa cgttgctgat cttgccgcgc tttcagtgcc tcttccagtg aacactggac
    53281 aaaatgaatc ggctgaccga gcggaatttg cgccagatgg tacatatcag cctcaatgat
    53341 acaggcaata cgtggataac caccggtggt ctgtgcgtcg ttcatcaaca caatcggctg
    53401 cccgttatgc ggcacctgca ccacgcccgg taacaaaccg tgagataaca gttcgcgatc
    53461 ggtgatgcgt tttaaaattt gcccctgtaa acgatagccc attcggttac tttgcgagct
    53521 aagctgccag ggcgaacgcc agaatgcatc ctgcgaggcg cgatcgaact catgatattc
    53581 cggccccggc aaggcgcgaa tgcggttgcc ccacagcagc tgtttaacgc cctgcgcttc
    53641 cataaaatca cgcttcgatt tgccaatcgg gagtcggtca ccatccttca gtaaacggcc
    53701 ttccagcccg ccaatcccca ctttgagatc ggtgctgcat gaccccatta ccggcggaac
    53761 atcaatacca cccgcgaccg ccagataact gcgcatcccg tgctgcgggc gtttaagcgt
    53821 taaacgctgg cctgctttca tcggcaatcg ccagccggtc cagacggcgt tatcatccaa
    53881 ccgcgcttcg caacctgcac ccgtcagggc aaaccaccca tcagtttcga actcaactgt
    53941 taactgaccg agcgtgatct ccagcgcggg ggcattggcg tcattaccca ccagcaggtt
    54001 agcaatgcgc agcgcaggca tatccagtgc gccacagtgg ctgatacccg actggcgaaa
    54061 accgtgacga ccgccatcct gcacagtggt gtacatgccc gcacgaataa tcttcagcat
    54121 attccctcct tctgcggtac aaatcgcacg ctgtctcccg gacgtaataa gatgggttcg
    54181 tcacgcgccg gatcaaacag gctgagtgag gtatgaccaa tcaactgcca gccacccggc
    54241 gttgccagcg gataaacacc agtctgcggc ccgccgatcc cgacagaacc tgccggaacg
    54301 agcaagcgcg gttcagcgcg ccgtggcgtg tgttgttgtt ccggcaaact cccgagatac
    54361 gggaagcccg gttgaaaacc taaaaaccag accacgtatt ccacggagga gtgcaattca
    54421 acaacctgtt tttcgctcaa cccgcaatac gccgcgacca ccgccaaatc cggtccgcct
    54481 gcaccaccgt aaaccaccgg aatttcaata aagcgagact caggttccag ggcctcgctc
    54541 tcctcccacc agcgttgcaa acgctctatg gcatccagcg ccagcgactc aggattacgc
    54601 aaaatcaccg tgatattgtt catgccggga atggcttcaa ccacattcgg catatccacc
    54661 agacgctgcg ccagtcgcca gatccgcttc tggctagcca gcgtcaccgg cggttccagt
    54721 tccagcacta ccgccgtttc acctatcaga taacaacgcg ctcgttgcac tttcgatccc
    54781 tctgattatt cgttatgcag gattaggaat atcaataaag gtcacatcaa gatcggtatt
    54841 ttcattcagc cactcgctca atgcgcgaat accaccgcgt tcagtggcat ggtgacctgc
    54901 agcataaaaa tgcaatccct gctcgcgggc tgaatgaatg gtctgttcag aaacttcgcc
    54961 agtaataaaa gcatccacgc caaaacgcgc ggcgctatcg ataaaacttt gcccgccgcc
    55021 cgtgcaccag gcgacgcgct ggaccacctc aggtccggta tcgccacacc ataacggctt
    55081 acgtcccaga cgcgcttcaa tccaggaggc tagttccatt cccggcacag gcatggtcag
    55141 ctctccccac ggcaccaacg gctcaatttc gcccatgacc gtgatcccca gtaacgccgc
    55201 cagctgtgcg ttattgccca gctcaggatg tgcgtcaagc ggcaaatgcc agccatacag
    55261 gttgatatca ttcgccagca acgtttttaa acggttacgc ttcattccgc gaatgaccgg
    55321 agactctcct ttccagaagt agccatgatg cacaatgacc gcatccgcgc ccagacgcac
    55381 tgcctcatcg agcaaagcct ggctggcggt gacaccggta acaatttttt gcaccgtctc
    55441 tttgccttcc acctgcaaac cattcggcgc gtaatcacta atcgccgcgc tgttcagttt
    55501 ttcgttaatc agttgttcca gttcggtgtt tttcatcttc gcgccttccg tattctgata
    55561 gctacaccgt taacatagcg ttacctcgcc tgttcgtcga tagccaatct tttgacatat
    55621 taagaaacaa acccgacgcc aaaagacgca aattgtttat atcatcacgc ttcagctaat
    55681 gtatatttat gcaatcagac agcatattgc ataaataatc agttctgtat caaagtttta
    55741 actttaagga aacacaagca cgccgcgcaa ctattacgtg acgttgccgt caattgtgga
    55801 attattatga ataaacacgc atcacaaccg cgcgctattt actatgtcgt tgcgctgcaa
    55861 atctgggaat attttagctt ttacggcatg cgtgccctgc tgattctcta tctcaccaat
    55921 caactaaaat acaacgatac tcacgcctac gagttattta gcgcctactg ttcgctggtg
    55981 tatgtcacgc caatcctcgg tggctttttg gcggataaag ttctcggcaa tcgcatggcg
    56041 gtgatgctgg gggcgttgtt gatggcgatc ggtcatgtgg tgctgggtgc cagtgagatc
    56101 catccgtcat tcctctatct gtccctggcg attatcgtct gcggctatgg cctgtttaaa
    56161 tctaacgtaa gctgtctgct cggcgagctg tatgagccaa ccgatccgcg tcgtgatggc
    56221 ggtttctcgc tgatgtatgc ggcgggtaac gtggggtcta ttatcgcacc tatcgcctgt
    56281 ggttacgccc aggaagagta cagctgggcg atgggctttg gcctggcggc ggttggcatg
    56341 atcgcgggtc tggtcatttt cttatgtggc aatcgtcatt tcactcatac ccgcggcgtt
    56401 aacaaaaaag tactgcgtgc gacaaacttt ctcctgccga actggggatg gctgctggtt
    56461 ctgctggtgg caacgcctgc actgattacc gtgctgttct ggaaagagtg gtcggtatac
    56521 gccttaattg tcgcgactat tattggcctg ggtgtactgg caaaaattta tcgcaaagca
    56581 gaaaaccaga aacagcggaa ggagctgggg ctgattgtga cgctcacctt attcagtatg
    56641 ttgttctggg ccttcgcaca acagggcggt agctcgatta gcctttatat cgaccgcttc
    56701 gttaaccgcg atatgtttgg ttataccgtt ccgaccgcga tgttccagtc gattaatgcc
    56761 ttcgcagtta tgctgtgcgg tgtgttcctg gcgtgggtgg ttaaagaaag cgtcgcaggt
    56821 aatcgtaccg tgcgcatctg gggaaaattt gctcttggcc ttggcctgat gagcgccgga
    56881 ttctgcattc tgaccttaag cgcccgctgg tccgcaatgt atggtcactc ttctctgcca
    56941 ctgatggtgt taggcctcgc ggtgatgggc tttgcggaac tgtttatcga cccggttgcc
    57001 atgtcgcaaa ttacgcgcat tgaaattccg ggcgtgaccg gcgtattaac cggcatctac
    57061 atgctgcttt ctggcgcgat cgcgaactat ctggcgggcg tgattgccga tcagacatcg
    57121 caggcttcgt ttgatgcttc cggggcgatc aactactcca tcaatgcata tattgaagta
    57181 tttgatcaaa ttacctgggg cgcactggcg tgtgtaggac tggtactgat gatttggctg
    57241 tatcaggcgc tgaaattcag aaaccgcgcg ctggcgctgg agtcataata ggccagcacc
    57301 gatgcctgat gcgacgcttg acgcgtctta tcaggcctac gtatttcctg caatttattg
    57361 aatttgcaca aatttgtagg ctggattagg cgttcacgcc gcatccggca tctgggctcg
    57421 attgcctgac gcgtctgtta tttccccttc cgcgccgtct catacgctgc caacgtttgt
    57481 actctcgcct ctttgtgctc gactatcggt tgcggataat ccagcttcac acctgctttc
    57541 tgcgcccact tccacggctc atgcaccgct ttccctggta catcgcgcag ctccggtaac
    57601 catcgacgaa taaactcgcc ctcacggtca aatttctcgc cctgagttat aggattgaaa
    57661 atacgaaaat acggcgcagc atcagtaccc gtagaagccg cccactgcca gccaccgtta
    57721 ttagctgcca aatcaccatc aatcagctgc gacataaaat atcgctcgcc ttcgcgccag
    57781 tcgatcaaca aatctttcac cagaaaactg gctgtaatca tccgtagccg gttatgcatc
    57841 cagccagtgc tgttaagctg acgcatagcg gcatcaataa tcgggtatcc cgttttgcct
    57901 ttctgccagg cctgtaaatg tgcgggattg ctttgccact gcacacgatc cgtccaggca
    57961 ataaatggac gatgtttaca caacgagggg taatacgtca tcagatgacg gtagaactcg
    58021 cgccagatca gctcactaag ccagacacta ccggccccac cgtccagcgc ctgcggctgt
    58081 tccgccaaca agcgatgcaa acactggcga ggcgataacc cgcccgttgc caagctggcg
    58141 gacaaacggc tggtgccttc cactgccgga aaatctcgtt gttgctcata ctctccagca
    58201 ccgttctcgc aaaactggcg taattgcgca atcgccgctt tttcttccac cgggaaatgc
    58261 gcagtatcga aagactgacg aggataattc agcgtaatgg atggcgcggg ctttatcgat
    58321 ccgctactac gaacttttgg tgcagcgacg cactccggca tcccttcccg cagccgtttc
    58381 agccaggcat tcttaaaagg cgtaaagact ttgtacatct cgtgattgcc ggtcatcacc
    58441 gcccccggcg gcaggatcac gctgtcatca aatccttcac acaccacgtt acgcagcgct
    58501 ctttcaacgt ccacatcccg cgctcgctca ttcacctcat actgatagtt ataaaaaaga
    58561 tgagtaacgc tgttttccgc gcacacctgt ttaactattt cgacactggc gacaaagtca
    58621 tccacttcac ggaacaataa aggaatacct ttttccgcca gcgcgatttg tagcccattc
    58681 agttgagcat tgataagttc agcctgacgc ggcgacatgt tatgcgtctc ccactggcgt
    58741 ggtgtagcga tatacagcgc cagcacgcgt gcagacgaat tgcggcaggc ggcagccagt
    58801 gcgagattat cgtgcagacg taaatcctgg cgaaaccagg ccagatgggt agtcataaaa
    58861 ctcctgaatg gcgcaagtta atcacacgcg gccaaagttc gaaataacgc tgccctgaaa
    58921 gccaggcgtc aggataaagc gccatatagt gtttgatacg catcagcggc gcaagaagtg
    58981 gttgctcgcc acggcgataa ctgtcgataa gcgcagccag ctgctggcgt tctgtggaat
    59041 caatatgcgg gcgaaaataa ccctgaacgt gcatcaaaac gttggtgtga tcgcgcgggt
    59101 tggcgggatg agaaagcaaa acaattacgc gttggcggta ctggttgtaa aaatcatcca
    59161 gattttgcca ctgatgaata tcagccacaa acgggccaag tttgcggtac tcgggctgag
    59221 aatgggccag aaagacgagt ttatagcggc tgtgataatc gagaagcgcc ccgcgcgtta
    59281 agccatgttc tttcagtcga tgtaactcat gaagggcgaa gatacggatt agggtgctgt
    59341 catcaaatcg ttgtagattc attactgctc ctgaacggta tacaggagta agtgtagacg
    59401 ttggtaaatg atgtttgtga agttaatccg ctagcagggc aagattacga aagcgcgctc
    59461 cccgcaagca actaatcaag gtagttaatc atggctgaac acgatattac gccagaagtc
    59521 accatttcca aaacacagcg ccgctataaa gtcggctatg tcagagcccg ccaccaggac
    59581 cgcaaaaccg gtatagcccg gtattatagc ctgcacctta agggcgactg gctgaaagag
    59641 gccggatttg ataccggacg gggtgtcacg gtgaagattt cggagggggg cctgaccatc
    59701 atcgctgaca gtgacgaggt gcaggaactg cgggaggaac tttatcaggt taaacaggta
    59761 gttaagggga tgcgggatgg gatgtttagt gtgctgaacg aaagttaaat attaaaaaag
    59821 ctgggaagat ttaaaacctt cctagctttt tattctgtct tattataatg tatactcatc
    59881 atcttccgat gaatttataa attgctccaa gtttacatga agagtactta acctagtttt
    59941 taactcatta agtgaagcga taatttcata ttttccaaca taatcatatg atggatcgta
    60001 ttcattatct atgtcagaag ctaaaaatga tgatattcgt agtttaaatt tagcttcatc
    60061 tttattacat tcattggcat aaggtgaatg ccctatcata gcatattcct ctaatctaaa
    60121 aggataagat ggttttaatt catcgatcaa tgaaatttca atccatgttc tatatccagg
    60181 cattaatgga ctaatttctt tgagcaaaac ctttccactt aataatgtat tgagattgtt
    60241 cattattatg tctccctcgg gaaaaatata tgtctaacac ttccaccatg ttgatgaact
    60301 tgtaaatgtg gtaaatcacc atcaggattt cctggtccat gacctgacac tctatatgga
    60361 ggttttgtta cagaaggatc ttttatatca tcccaatgat atgaacctgc ttttgtttta
    60421 tttccagtaa gatattccat taagttctta gtgctggggc ctgactcacc tgtcatattt
    60481 ttataattat gacctaaata tctatccata aacaattgtt cagcgtcact tctagaccta
    60541 acagtaactt tggtttcact actatctaat aaacgattcg ctttttggtt actaacagca
    60601 catggagtta atccaagtgg atcagcatat tttagaggat taggtgcata actgtagaga
    60661 tttaaaccgc ctctcaaccc tatcgggtca gttaccatga accgcccgac atccggatca
    60721 tagtacctgt gcagattgta gtgcagcccc gtttccctgt cgaggtactg tccctggtag
    60781 cgcaggttct gctccacccc gccgtggtcc ttctcctgta tcgtgttacc ccagacctga
    60841 taccccgtct cccacacgat tttcccgtcg ctgtccgtca tctcttccgg cgcgccgttc
    60901 acatccgtgt gaaaatacag tatccggttc gggccctctt tgcccgcctt atccacccgc
    60961 gccagcggtt cataactccc ctggtccctg tagatgtaca ggctgcctgt tccctccggc
    61021 ctgctctcct gcgccagccg cattccgtcc cataaaaatt ttgtccggtt gtacggctta
    61081 ccgtcagggc tgatgtgctg tttttccgtc cgcctcccca gtgcatcgta cacataccgg
    61141 tatgtccccc gcgctgtcgt cacttccacc atccggtgtt ccgcatcata acggtaactc
    61201 tgcgttccgc tccggccctc cttttccacc gcacggccaa attcgtcata acggtagtgc
    61261 acaccacggt aagacatcag ccggttgaac ggcaccacgc tgttttctgc cgtggccctt
    61321 tcccccgcgc gcctccccag caggtttccc gccgcgtcgt actgccagct cgccagatac
    61381 ccctcatcct ccgaacgaca cgccgtgatg ctccccgtgg ggtcgtagaa gtaatccgtt
    61441 tccccccgcc ggctgtgccg cgtcttcagc agttcatcct cgccactgta gctgtactgc
    61501 cggtccgtga acgtctccgg taccaccccg cgcatcccgc tgtatgtctc cctgcgcttc
    61561 agccgcccgc cggggtcgta aagctgccgc gtgctcagtc gcccctggct tctgagcact
    61621 tcacggtgca ggtggtcgcg cgtgaactcg ctgatgacct cccggtccag atttatctgc
    61681 tggagatggc cgctgccgta gtacagatgg cgctgcgttc tgccgtccgg aaaggtggtg
    61741 gcagtccggt tgcccagttc atcgtactgg tgggtcagga tgccctgcgc cgtctcctcg
    61801 ctcagcatcc gccccagtct gtcgtagctg aagcggataa cttccggcgc gtcagtttcg
    61861 tgccgctggc gacggatttc cagtagctgg tctgccgcat catggacgta ttccgtacgg
    61921 ccatcggctg tctcttttgc cgtgagccgg cccgccgcat cacgcgccag ccggtggcgg
    61981 atttcaccgc cccggttccc ggtgaacacc acttccgtga ccgcgttcag cgcgttgtag
    62041 cggtacgact ggcagcagcc gtccgggttt atctgctccg ccagacggtc cagcacatca
    62101 tggcggaacc agtagcgttc tccgttctcg ttcgtgagcg tgaccagacg cccgtaagcg
    62161 tcgtacccga atgccgtcct gcgccccatc gcgtccgtcc ggctgcgcac ccggccctgc
    62221 ccgtcccgct gccagcgcgt tatcctcccc atcgggctgg tgtactttac cagcctgccc
    62281 gccgcgtctg actggtaatt caccgtgcgg ccgtccgcca gtatcacctc cgtcagatga
    62341 ccgctgtcgt catacccgta gcgcgtgctg tggctctgcg catccgtcat tctgataagc
    62401 ctcgtgcgtt cgtcgtagaa ccacgccgtc tgcgagccgg aacagtccgt gtgccgcatg
    62461 agctgcccgt cttcattcca ctggaggtat ttattccccc cgcgcgcatc tgttatctgc
    62521 accacctggc cgtggtggtc atactgatac cgggtgttct gctgcagcgg gtcggtcacg
    62581 gcaagaaggt tgccgcgttc atcgtacaca cagcgccagg tgtttcccgc cgcgtccacc
    62641 tccgtttccg gctggtgcca caccgggtgc cactgcgcct gctccacccg ccccagcggg
    62701 tcgtgcgtct ccgtgatatg cccctgcagg tcatacacat agcgccattt gccgccctga
    62761 gggtcggtca tccccagcaa cagtcgttct tcgtcgctcc agcggaatgt cgtcacctgt
    62821 ccggcttcgt cctggtacgc cgtcacctga ttctgcgcat cccactggtg ataacgtgtg
    62881 cgccccagtc cgtccgtcac gcacagcctg cgctgcgcca ggtcccacgc cagccggtac
    62941 tgttcaccgc tgtccgtgtg gtgccccgtc acccgccatg ccgcgtcatc cggaccttca
    63001 taccagcccc actggtaata atacccttcg ccgcccggct ggcggtgcag cgtcatgcag
    63061 tggttttcgt cgtaagcaaa ctcacgcagc aggtgccccg tgctgtcgta cacgccgttc
    63121 aggtggccgg cctcgtcata gctgtaacga cgcagcagta aaggtgcagt ctcagggaaa
    63181 tgctgataaa tctgtgtcac ccgccgggga tgcgccgcca gttcgtaata cagacggatg
    63241 cacggacgct gctgttcgcc gctgatttcc acaatgcggc cccggtcgtc gtagtacaga
    63301 ttcagacagt tgctgttacg gtcgcccagc attttcagcc gctggcgctg cgggtggtgc
    63361 gggtcagcct caaacagaaa acactgcccg tcatcgctgc tgataagcca gtgcccctgc
    63421 tcaccacgcc ttaccgccag cccttcaccg gggctgtaaa acccgccccc cggcagtacg
    63481 gcttccatat ccaggcgtcg ccccatgccc gacacaaagg tcatgcagtt ttcgtctgcg
    63541 ctacccggtg tgcacacgag gctgacttca tacattacac tccagccctg accaaacatt
    63601 ccttccgtac gttcgtcacg actgctgtac acgcgctgcc agtccagcgg aaagtggccc
    63661 ggcagactga aatcgaagtc atcgtcatcg gccaggtatt tggccccggt ggcggcattg
    63721 accggatgcg gaagggcact ggtggccttc tgcgtcagca tgctcatccc gacactggca
    63781 gcaaaacagg cgattttaga aagcattttg cccgggcgaa gaaacatcag cgccacggtt
    63841 gccaccaggg ttatctgcga cctgccgctt ctgatatccc tgaccaccag taacggaccg
    63901 ccaatgcgca catccggtga cacaaacgcc ccgttccccg gctcattcac caccctggcc
    63961 tcgcaggtac agcgtgcccc gctgcgtgtc gccggctggc tgttgatacg cacatcccgc
    64021 gcgccttccg cgatatattc cgctgcgggc ggctcgtcat cgcctttgaa cagattactg
    64081 atgctgccaa taaccgcatt cactccctga aaggcggttt ccagcatacc gcccggattc
    64141 agcaggttaa gggccgttcc cagggggtca tccagaaagg cggctttttt ttgtgcgaga
    64201 aactgcgtca gtgaggaagg atgtttatgg cagtcgattt tatcgtcagg ggtcggggtg
    64261 gagcccgggg ccgcactggc cacagtgggc tgccatagtt ccgacacaaa atgtgtcacg
    64321 ccatccacca tttcagaacg cccttcttcc gtactgagct ggtcgtgcag agcccgggac
    64381 atcagcacag gccctgtcac cgggttaacc gccagggcta tggcccccgc catttccaga
    64441 tattccccgg ccacatcccg gaacgttctg gcatcctcct gacgctgcgc ttcttcctgt
    64501 tctgccttcg cctgcgcttc ctgcgcctct atgtcctgcc gcgatgccgc agtcgccgct
    64561 gcccgcgcgg caggtttgct gttgatccat gtgtcaccag agccggtgag gatgtaacct
    64621 tcaatctgag gcgggaaaag catatttgcg agggctgaac aggcttcgct gaccttttct
    64681 gtcacgccgg tgtacaccat cgccacaccc gccacaaaac cggtcagcac gacggccgtg
    64741 ccccccatcg tgatccccac tgacgtcgcc agtaacgagg ttgtgccgac aatgagggca
    64801 tcaataacga catttgcagc cacctccagc accgccccca gcacatctgc cataaacgac
    64861 gtatgcagca gggcatcccc ctgtcttgcc gcccagaatg cctcttccat gtcccgttcc
    64921 ctgtatgatt aattcgtcct gacttcctgc cgcatcctga agcgcagatt ctttttaatt
    64981 tcagcccagc gcgtcatatc tgccggggtc agcggtgtgg ttctggcgta ggtgaaggcc
    65041 atcatgaccc gctcacccgg caatac
//
LOCUS       DAJTYF010000010        60673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTYF010000010 DAJTYF010000000
VERSION     DAJTYF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.010"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..320
                     /locus_tag="OO590_000733"
     CDS             <1..320
                     /locus_tag="OO590_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6636208.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
     gene            433..1227
                     /gene="panB"
                     /locus_tag="OO590_000734"
     CDS             433..1227
                     /gene="panB"
                     /locus_tag="OO590_000734"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6636209.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1239..2090
                     /gene="panC"
                     /locus_tag="OO590_000735"
     CDS             1239..2090
                     /gene="panC"
                     /locus_tag="OO590_000735"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6636210.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2164..3066)
                     /gene="rpnC"
                     /locus_tag="OO590_000736"
     CDS             complement(2164..3066)
                     /gene="rpnC"
                     /locus_tag="OO590_000736"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6636211.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3340..3720
                     /gene="panD"
                     /locus_tag="OO590_000737"
     CDS             3340..3720
                     /gene="panD"
                     /locus_tag="OO590_000737"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6636212.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3724..4953)
                     /locus_tag="OO590_000738"
     CDS             complement(3724..4953)
                     /locus_tag="OO590_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6636213.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5017..5457)
                     /locus_tag="OO590_000739"
     CDS             complement(5017..5457)
                     /locus_tag="OO590_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6636214.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5562..6332)
                     /locus_tag="OO590_000740"
     CDS             complement(5562..6332)
                     /locus_tag="OO590_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6636215.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6329..7255)
                     /locus_tag="OO590_000741"
     CDS             complement(6329..7255)
                     /locus_tag="OO590_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6636216.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7364..8026
                     /gene="can"
                     /locus_tag="OO590_000742"
     CDS             7364..8026
                     /gene="can"
                     /locus_tag="OO590_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6636217.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="OO590_000743"
     CDS             complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="OO590_000743"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6636218.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8809..11199
                     /gene="gcd"
                     /locus_tag="OO590_000744"
     CDS             8809..11199
                     /gene="gcd"
                     /locus_tag="OO590_000744"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6636219.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHAFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="OO590_000745"
                     /pseudo
     CDS             complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="OO590_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12961..13308
                     /locus_tag="OO590_000746"
     CDS             12961..13308
                     /locus_tag="OO590_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6636220.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13414..14280
                     /gene="speE"
                     /locus_tag="OO590_000747"
     CDS             13414..14280
                     /gene="speE"
                     /locus_tag="OO590_000747"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6636221.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14296..15090
                     /gene="speD"
                     /locus_tag="OO590_000748"
     CDS             14296..15090
                     /gene="speD"
                     /locus_tag="OO590_000748"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6636222.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15128..15490)
                     /locus_tag="OO590_000749"
     CDS             complement(15128..15490)
                     /locus_tag="OO590_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6636223.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="OO590_000750"
     CDS             complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="OO590_000750"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6636224.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18618..20471
                     /locus_tag="OO590_000751"
     CDS             18618..20471
                     /locus_tag="OO590_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR6636225.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNSITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQATASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20542..21972)
                     /gene="lpdA"
                     /locus_tag="OO590_000752"
     CDS             complement(20542..21972)
                     /gene="lpdA"
                     /locus_tag="OO590_000752"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6636226.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPKAKKK"
     gene            complement(22297..24189)
                     /gene="aceF"
                     /locus_tag="OO590_000753"
     CDS             complement(22297..24189)
                     /gene="aceF"
                     /locus_tag="OO590_000753"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6636227.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPVAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24204..26867)
                     /gene="aceE"
                     /locus_tag="OO590_000754"
     CDS             complement(24204..26867)
                     /gene="aceE"
                     /locus_tag="OO590_000754"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6636228.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27028..27792)
                     /gene="pdhR"
                     /locus_tag="OO590_000755"
     CDS             complement(27028..27792)
                     /gene="pdhR"
                     /locus_tag="OO590_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6636229.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28333..29706
                     /gene="aroP"
                     /locus_tag="OO590_000756"
     CDS             28333..29706
                     /gene="aroP"
                     /locus_tag="OO590_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6636230.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29749..30603)
                     /gene="ampE"
                     /locus_tag="OO590_000757"
     CDS             complement(29749..30603)
                     /gene="ampE"
                     /locus_tag="OO590_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6636231.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30600..31151)
                     /gene="ampD"
                     /locus_tag="OO590_000758"
     CDS             complement(30600..31151)
                     /gene="ampD"
                     /locus_tag="OO590_000758"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6636232.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31239..32132
                     /gene="nadC"
                     /locus_tag="OO590_000759"
     CDS             31239..32132
                     /gene="nadC"
                     /locus_tag="OO590_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6636233.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNDKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32335..32775
                     /gene="ppdD"
                     /locus_tag="OO590_000760"
     CDS             32335..32775
                     /gene="ppdD"
                     /locus_tag="OO590_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6636234.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32785..34170
                     /gene="gspE"
                     /locus_tag="OO590_000761"
     CDS             32785..34170
                     /gene="gspE"
                     /locus_tag="OO590_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCR6636235.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAVQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34160..35362
                     /gene="hofC"
                     /locus_tag="OO590_000762"
     CDS             34160..35362
                     /gene="hofC"
                     /locus_tag="OO590_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6636236.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35397..36440)
                     /gene="guaC"
                     /locus_tag="OO590_000763"
     CDS             complement(35397..36440)
                     /gene="guaC"
                     /locus_tag="OO590_000763"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6636237.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36602..36640)
                     /locus_tag="OO590_000764"
     CDS             complement(36602..36640)
                     /locus_tag="OO590_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636238.1"
                     /translation="MIIKTLKMSARK"
     gene            36665..37285
                     /gene="coaE"
                     /locus_tag="OO590_000765"
     CDS             36665..37285
                     /gene="coaE"
                     /locus_tag="OO590_000765"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6636239.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLIENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37285..38028
                     /gene="zapD"
                     /locus_tag="OO590_000766"
     CDS             37285..38028
                     /gene="zapD"
                     /locus_tag="OO590_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6636240.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38038..38235
                     /gene="yacG"
                     /locus_tag="OO590_000767"
     CDS             38038..38235
                     /gene="yacG"
                     /locus_tag="OO590_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6636241.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38335..38724)
                     /gene="mutT"
                     /locus_tag="OO590_000768"
     CDS             complement(38335..38724)
                     /gene="mutT"
                     /locus_tag="OO590_000768"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6636242.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38784..41489)
                     /gene="secA"
                     /locus_tag="OO590_000769"
     CDS             complement(38784..41489)
                     /gene="secA"
                     /locus_tag="OO590_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6636243.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKLGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41551..42063)
                     /gene="secM"
                     /locus_tag="OO590_000770"
     CDS             complement(41551..42063)
                     /gene="secM"
                     /locus_tag="OO590_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6636244.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42294..43211)
                     /gene="lpxC"
                     /locus_tag="OO590_000771"
     CDS             complement(42294..43211)
                     /gene="lpxC"
                     /locus_tag="OO590_000771"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6636245.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43312..44463)
                     /gene="ftsZ"
                     /locus_tag="OO590_000772"
     CDS             complement(43312..44463)
                     /gene="ftsZ"
                     /locus_tag="OO590_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6636246.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44524..45786)
                     /gene="ftsA"
                     /locus_tag="OO590_000773"
     CDS             complement(44524..45786)
                     /gene="ftsA"
                     /locus_tag="OO590_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6636247.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45783..46613)
                     /gene="ftsQ"
                     /locus_tag="OO590_000774"
     CDS             complement(45783..46613)
                     /gene="ftsQ"
                     /locus_tag="OO590_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6636248.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46615..47535)
                     /gene="ddlB"
                     /locus_tag="OO590_000775"
     CDS             complement(46615..47535)
                     /gene="ddlB"
                     /locus_tag="OO590_000775"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6636249.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47528..49003)
                     /gene="murC"
                     /locus_tag="OO590_000776"
     CDS             complement(47528..49003)
                     /gene="murC"
                     /locus_tag="OO590_000776"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6636250.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49057..50124)
                     /gene="murG"
                     /locus_tag="OO590_000777"
     CDS             complement(49057..50124)
                     /gene="murG"
                     /locus_tag="OO590_000777"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6636251.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNGWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50121..51365)
                     /gene="ftsW"
                     /locus_tag="OO590_000778"
     CDS             complement(50121..51365)
                     /gene="ftsW"
                     /locus_tag="OO590_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6636252.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51365..52681)
                     /gene="murD"
                     /locus_tag="OO590_000779"
     CDS             complement(51365..52681)
                     /gene="murD"
                     /locus_tag="OO590_000779"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6636253.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52684..53766)
                     /gene="mraY"
                     /locus_tag="OO590_000780"
     CDS             complement(52684..53766)
                     /gene="mraY"
                     /locus_tag="OO590_000780"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6636254.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53760..55118)
                     /gene="murF"
                     /locus_tag="OO590_000781"
     CDS             complement(53760..55118)
                     /gene="murF"
                     /locus_tag="OO590_000781"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6636255.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIARTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEDCHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55115..56602)
                     /gene="murE"
                     /locus_tag="OO590_000782"
     CDS             complement(55115..56602)
                     /gene="murE"
                     /locus_tag="OO590_000782"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6636256.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56589..58355)
                     /gene="ftsI"
                     /locus_tag="OO590_000783"
     CDS             complement(56589..58355)
                     /gene="ftsI"
                     /locus_tag="OO590_000783"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6636257.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58371..58736)
                     /gene="ftsL"
                     /locus_tag="OO590_000784"
     CDS             complement(58371..58736)
                     /gene="ftsL"
                     /locus_tag="OO590_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6636258.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58733..59674)
                     /gene="rsmH"
                     /locus_tag="OO590_000785"
     CDS             complement(58733..59674)
                     /gene="rsmH"
                     /locus_tag="OO590_000785"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6636259.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59676..60134)
                     /gene="mraZ"
                     /locus_tag="OO590_000786"
     CDS             complement(59676..60134)
                     /gene="mraZ"
                     /locus_tag="OO590_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6636260.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14533 a        16800 c        14918 g        14422 t
ORIGIN      
        1 aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa
       61 aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga
      121 aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa
      181 caggaggagt ttacccggat caagatacag gtataacata ccctctccat ttccaggcca
      241 cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt
      301 tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt
      361 gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta
      421 tcaggatacg ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa
      481 aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa
      541 gggcttaacg tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc
      601 actctgccag taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca
      661 ccaaactgcc tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc
      721 tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt
      781 gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac
      841 ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc
      901 aatgaagcga gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag
      961 ctgctggtgc tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg
     1021 atcccggtta ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     1081 gacgcctttg gtattaccgg cggtcacatt cctaaatttg ctaaaaattt cctcgccgaa
     1141 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat
     1201 ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc
     1261 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc
     1321 ctactatggg taatctgcac aatggccata tgaagctggt cgacgaagcc aaagcccgcg
     1381 ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc
     1441 tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg
     1501 atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt
     1561 acgttgacgt tcctggcctt tcaaccatgc tggaaggtgc cagccgtccg ggacattttc
     1621 gcggcgtttc gactattgtc agcacgctgt tcaacctggt ccagccggac atcgcctgct
     1681 tcggtgaaaa agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct
     1741 tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt
     1801 cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt
     1861 taagttcgat tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca
     1921 ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg
     1981 ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct
     2041 ggcttggcga tgctcgcctg atcgacaaca aaatggtcga gctggcgtaa tacttaactg
     2101 gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt
     2161 aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc
     2221 aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc
     2281 ctgtcttccc tgcggaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2341 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2401 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2461 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2521 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2581 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     2641 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     2701 ataaaacagt atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg
     2761 catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     2821 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     2881 gtccgtgctg tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     2941 gttgagatca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3001 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
     3061 atccatcttc cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc
     3121 tatcgtcagc cacctcttta ttgtttaccg agcagcattc ataaaaatat caattcgtta
     3181 caggcgttac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga
     3241 tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
     3301 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3361 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3421 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg
     3481 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3541 attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
     3601 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat
     3661 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     3721 ttgttatccc tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct
     3781 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     3841 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     3901 gccgccaaac ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg
     3961 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4021 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat
     4081 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4141 acgtttgatg cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac
     4201 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4261 cgcagggaga ttgatcgtat tcttcacgta accttccagc tgagccatgc tcagtgtcgc
     4321 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4381 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4441 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4501 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4561 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg
     4621 cgtatctttc caggtaatta agttctgatt actcagcggc ttgttaaggt cacccaaacc
     4681 gtcttcaact ttttgtcgcc cctgaactgg ctcgagatgc cctttatcga taaacccttt
     4741 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctctacgg caatgatttg
     4801 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc
     4861 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     4921 catcagcagg agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg
     4981 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
     5041 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5101 cagttcgaca atacactcac gaaactctga actggtcatg gtttcacggc aagccatcat
     5161 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5221 taataatgaa gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc
     5281 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5341 taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
     5401 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5461 cttcctcctt agccgaaaat cagcctaccc tgaccattgt tgataaaggg tgatagccgt
     5521 caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5581 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     5641 ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg
     5701 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     5761 agtgagtaaa agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     5821 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc
     5881 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     5941 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6001 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6061 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc
     6121 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6181 ccaatacgcg aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6241 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6301 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6361 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6421 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt
     6481 ccagcgtcgc agtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6541 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6601 aggtattttc caccagctca ccatgttgaa taatgccgat attgagacac agcatttctg
     6661 cttcttccag gtagtgtgtg gtaagaatga tggtggtgcc tttgtcgttt aaatccttca
     6721 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     6781 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     6841 cagataacat acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
     6901 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     6961 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7021 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7081 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7141 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7201 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7261 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7321 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7381 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7441 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7501 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7561 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7621 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     7681 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     7741 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     7801 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
     7861 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     7921 cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg
     7981 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8041 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac
     8101 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8161 atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc
     8221 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8281 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8341 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8401 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8461 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8521 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8581 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     8641 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     8701 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaatct
     8761 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     8821 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     8881 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     8941 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
     9001 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9061 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9121 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9181 gtggtcgcac tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag
     9241 gagatcaacg gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc
     9301 gatcaggact ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag
     9361 caaattcacg ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
     9421 gtgaagcagc cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc
     9481 gatacccttt acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc
     9541 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc
     9601 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
     9661 gattgcccgc gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct
     9721 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
     9781 atgccagaca ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa
     9841 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg
     9901 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa
     9961 gatccgaacg caatcccgtc tgacgaacac gcctttacct ttaactcacc aaactcgtgg
    10021 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10081 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10141 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10201 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10261 gtgccgatta tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat
    10321 ggcgaactgg tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    10381 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    10441 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10501 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg
    10561 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10621 attgccaacc caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac
    10681 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    10741 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    10801 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    10861 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg
    10921 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    10981 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11041 tggcagggtc gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc
    11101 aagcagtacg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11161 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagaaatcc
    11221 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11281 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11341 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11401 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat
    11461 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11521 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11581 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    11641 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    11701 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    11761 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    11821 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg
    11881 ccatccccat ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct
    11941 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12001 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12061 aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg
    12121 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12181 cggcggtcat cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct
    12241 gaacgatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12301 cgtcatcttc aatcaccacc agaccagcca gccccatcgc cacctgtcgc cggtcttgcc
    12361 atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga
    12421 gcgcttgcca cctggcggaa taattccctg tgggccgccg tcgacgtcac ccggtacttc
    12481 cagcccgtgc cagtgcaacg tcgtctcttc cgccagttgg ttgtagatat caaccgttac
    12541 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    12601 agttgcagtt ttctcgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt
    12661 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac
    12721 tgcacggctc cacagtggca aggctgaagc cacacccagt gcgacggaat atttcaagaa
    12781 atcacgacgt tgcatagtta tttccttatt cttaagcagg caaaacaaaa tcaaacgttg
    12841 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    12901 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    12961 atgaagacgt ttttcagaac agtgttattc ggcagcctga tcgccgtttg cgcaaacagt
    13021 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    13081 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    13141 tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagct
    13201 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc
    13261 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    13321 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt
    13381 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa
    13441 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    13501 accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    13561 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    13621 gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    13681 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    13741 gatgcgggtg tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac
    13801 gacgatccgc gctttcaact agtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    13861 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctg
    13921 ttcacctcag cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    13981 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc
    14041 agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt
    14101 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    14161 attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat
    14221 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gccgtcctaa
    14281 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    14341 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    14401 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    14461 tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt
    14521 gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca
    14581 gaacaccccg gcccactgcc agaaacggtg gttgctcatc tcgataaaag tcatatttgc
    14641 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    14701 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    14761 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac
    14821 ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    14881 atgaaggcgc tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc
    14941 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat
    15001 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    15061 tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca
    15121 gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct
    15181 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat
    15241 cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg
    15301 tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt
    15361 gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    15421 ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt
    15481 cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat cgcgccattg
    15541 atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    15601 aaatgcccgg gaatctcatt caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt
    15661 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    15721 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    15781 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    15841 gcagaagcca ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag
    15901 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag
    15961 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc
    16021 agttgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    16081 ttgtgcgcat ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa
    16141 ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc
    16201 ggatcgttcg gagcacacag gattggctct ttaatctccg ccagatcgat gtcgatcact
    16261 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16321 atgccctgaa tacggcgttc caaggtacga cgatcgccgt aaccttccgc gatcatccac
    16381 ttcagcagga cgatgttgga gctcaggtat tcgatgatcg gttctttgtt cagcttgata
    16441 gtacaaccag cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact
    16501 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16561 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga
    16621 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    16681 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    16741 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    16801 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    16861 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    16921 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    16981 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    17041 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    17101 attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17161 acatcactgt gcggcagacc aagtgcttca cgagctttgg tggtcaggcc acgcccgata
    17221 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17281 aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17341 tcaatcacgt cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg
    17401 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17461 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17521 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17581 tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata
    17641 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    17701 gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta
    17761 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    17821 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    17881 ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca
    17941 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    18001 tcgatagctt tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc
    18061 aggaagcctg cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    18121 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg
    18181 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18241 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18301 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18361 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18421 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18481 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18541 cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt
    18601 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    18661 tttgcagtgc cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg
    18721 aagcgcctgt agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc
    18781 ttcctgcaac ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca
    18841 ctgcacaaat agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc
    18901 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    18961 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19021 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19081 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19141 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19201 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19261 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19321 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19381 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19441 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19501 acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19561 atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg
    19621 gcgacaacat caatatcgac gtcaacaatt tcaacagtat caccggtgag catcttactg
    19681 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    19741 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    19801 tacctgctcc aacacgcgac agccagcgtc aggcgacagc aagtcagttt cagcaacgaa
    19861 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    19921 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    19981 tgacccagca gcaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc
    20041 gccaggcagt ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcag
    20101 cgcgccagcg tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    20161 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa
    20221 ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc
    20281 aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc
    20341 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    20401 tgaataacga gcaacggtca acagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    20461 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    20521 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcgggt tcggcaggtc
    20581 ggtaatgcta ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg
    20641 cgcgtggatg gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccagcccgat
    20701 ttcacccagc agctcgccgc cgttggtacc aacaatcgca ccaccgatca cgcggtgaga
    20761 ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc
    20821 agaagcagcc cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt
    20881 ctcagtcaga cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt
    20941 cggatcgaag tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac
    21001 acctttgtgt gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac
    21061 gttggtacgc agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc
    21121 tttgcctgcg tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc
    21181 gtaacgctgc ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc
    21241 tttcgcttca acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg
    21301 cttggtgaag actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc
    21361 aaccacgtca atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat
    21421 accgccaccc attaccagca ggcgttctgg cacttctttc agttccagcg cgtcagtgga
    21481 gtcccagata cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc
    21541 tgcaatgatc gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag
    21601 ggtgttagcc ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc
    21661 cataccagcc agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt
    21721 gtcaatatcg gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc
    21781 ttcgataact tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca
    21841 aacaccgcca agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca
    21901 acggaaggca gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat
    21961 ttcagtactc atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca
    22021 ggccgcagcg gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg
    22081 ggattctggc gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac
    22141 cctaaccacc accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta
    22201 aacaacaaac ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta
    22261 ccagaaaaaa gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc
    22321 agacagcgtg ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg
    22381 gtggtcgaag gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca
    22441 caccggctcc atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat
    22501 cggcgcgaag tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg
    22561 catttcgccc gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg
    22621 agacagctcg atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt
    22681 cggggtatcc accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc
    22741 ttccgacagc gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat
    22801 gatgaagaca accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg
    22861 ctgtttacgg aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg
    22921 gatcatcacc cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc
    22981 cacttcttcg atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg
    23041 gataccaccg ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta
    23101 agcctgaacg tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag
    23161 gttaacgcca aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc
    23221 gttttcagca aattcagatt tgccttccgc tttcgcagcc ggagctgctg ccggagcttc
    23281 agcttttgct gccggagccg gcgctgccgc ttcctgtttc gcaggagctg ccgcaggtgc
    23341 tgcgccttca acttcgaaga tcataatcag cgaaccagtt ttcactttat cgccaacgtt
    23401 gactttcagt tccttcacga cgcctgcaaa cggagccgga acttccatag aagctttatc
    23461 gccttctacg gtaatcagtg actgttcagc ggcaactttg tcgcccactt tcaccatcac
    23521 ttcggtcact tcaacttcgt caccgccgat atccggaacg ttaacttctt tcacgccagc
    23581 tgctggtgcg ggggtcgctg ccggagtcgc ttcctgttta gccgccggag ctgccgcgcc
    23641 tgcttcaccc gcgacttcga agaccataat cagcgagccg gtagacactt tgtcacccac
    23701 gttcactttg atctctttca cggtacctgc aaacggagcc ggaacttcca tagaagcctt
    23761 gtcgccttct acggtgatca gcgactgttc agcttcaact ttatcgccaa ctttcaccag
    23821 gatttcggtc acttcaactt cgtcgctgcc gatatccgga acgttaacgt cttttgccgc
    23881 cgcagccgcg ggtgctgcta ccggagctgc ttctttcttc tcttctgcct gagcaggtgc
    23941 agcgtctgct gcaccgtcgg cggaatcgaa aatcataatc agtgcgccgg tctgggtttt
    24001 atcgccaaca gagactttga tctctttaac gatacccgcc tgcggagatg gaacttccat
    24061 agaggctttg tcgccttcta cggtgatcag cgactgttcg gcttcaactt tgtcgcccac
    24121 tttgaccagg atctcggtga tttcaacttc atcagccccg atgtccggta ctttgatttc
    24181 gatagccatt attcttttac ctcttacgcc agacgcgggt taactttatc tgcatcgatg
    24241 ttgaatttgg cgattgcgtc agcaaccact ttcttatcga tttcgccacg tttagccagt
    24301 tcgcccagcg ccgcaaccac gacataagaa gcatcaactt cgaagtggtg acgcaggttc
    24361 tcacggctgt cggaacgacc gaagccatca gtacccagta cgcgatagtc gtcagccggt
    24421 acgtaagtac ggacctgctc agcgaacagt ttcatatagt cggtagatgc cactgccggc
    24481 gcgtcgttca tcacctgagc gatatacgga acgcgcggag tttccagcgg atgcagcatg
    24541 ttccagcgtt cacaatcctg accatcacgc gccagctcgg tgaaggaggt cacgctataa
    24601 acgtcagaac ctacgccgta atctttcgcc aggatctctg ccgcttcacg gacgtgacgc
    24661 aggatggaac cggagcccag cagctgaact ttacctttgc taccttcaat ggtttcgagt
    24721 ttgtagatac ctttacggat accttcctca gcaccttccg gcattgccgg catgtggtag
    24781 ttttcgttca gcgtagtgat gtagtagtaa acgttctctt gtttttcacc gtacatacgc
    24841 tccagaccgt catgcataat gacagcaact tcgtaagcgt aagccgggtc gtaagagata
    24901 cagttcggga tagtcagcga ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttca
    24961 ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga agccacgcgc ttgctggtct
    25021 ccagccgccc agcacagatc gccgatacgc tggaagccga acatcgagta atagatgtag
    25081 aatgggatca tcggcagatt gttcgtgctg taagaggtcg ccgctgccag ccaggaacac
    25141 cctgcgccca gctcgttgat cccttcctgc agaatctgac ctttctcgtc ttctttatag
    25201 taagcaacct gctcgcggtc ctgcggggtg tactgctgac cgttcgggct gtaaatacca
    25261 atctgacgga acagaccttc cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc
    25321 agacgatctt tgatcgactt gttcttcagc atcacgttca gagcacgaac gaaagcgata
    25381 gtggtagaga gctctttgct ctgctcttcc agcagcgcgc cgaagtcttg caggctcggc
    25441 agctcaagct tctcggtgaa gttcggctga cggcttggca gataaccgtg cagtttctga
    25501 cgctgagcgt gcagataggt atgctcttca gaaccttccg ggaaggtgat gtacggcagt
    25561 ttttcgatat ctgcatcaga caccggcaca ttgaaacggt cgcggatgtg acgcacgccg
    25621 tccatgttca ttttcttaac ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg
    25681 ccgtaacctt taatggtatg agcaaggatt actgtcgctt tgcctttggt ttcctgcgct
    25741 ttcttgaatg cagcgtagat tttcttcgga tcgtgaccac cacggttcag tgcccagatc
    25801 tgctcgtcag tccagtctgc aaccagtgct gcggtttcag gatatttacc gaagaagtgt
    25861 tcacgaacgt acgcaccatc tttcgatttg aaggtctggt agtcgccgtc aacggtttcg
    25921 ttcatcagct ggatcagttt accgctggta tctttacgca gcagttcatc ccaacggcta
    25981 ccccacatca ctttgatcac gttccagcca gcaccttcga agatgccttc cagttcgttg
    26041 atgatcttgc cgttaccggt gaccgggccg tcaagacgct gcaggttaca gttgataacg
    26101 aaaaccaggt tatccagttt ttcacgggta gcgatggtga tcgcaccttt ggattccggt
    26161 tcgtccattt caccgtcgcc gaggaacgcg taaacggtct gtttagaggt atctttcagg
    26221 ccacggtgtt ccagatattt caggaattta gcctggtaaa tagcaccaat cggacccaga
    26281 cccatagata cggtcgggaa ctgccagaat tccggcatca gtttcgggtg cggataggaa
    26341 gagaggccat tgccgtgaac ttcctgacgg aagttatcca gctgctcctg agtcagacga
    26401 ccttccagga aagcacgggc gtaaacgccc ggggagatgt ggccctggaa gtaaaccagg
    26461 tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt taaagcacac atcataaatg
    26521 gttgcggaag actggaagga cgccatgtgg ccgcccagtt cgaggtcttt tttcgacgca
    26581 cgcagcaccg tcatgatggc gttccagcgg atagctgaac gaatacggcg ttccagttcc
    26641 agattacccg gatactccgg ttgttcttca acggggatgg tgttgatgta gttgctgata
    26701 cctgtgcctg cggctacgtt tacaccgcct ttgcgggctt cagcaagcag ttggtcgatc
    26761 agatactgag cacgctcaac accttcttca cggatgaccg attcgatcgc ctggagccag
    26821 tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt ctgacatggg ttattcctta
    26881 tctatctaat aacgttgagt tttctggaac ctgtcccatt gaactctcgc cagaaagctc
    26941 aataagacag gttctacgtt tagttaccgc gcttttatat gcgcttgatt tacaacatct
    27001 tctggataat ttttaccaga aaaatcacta attctttcgt tgctccagac gacgcagaga
    27061 acgctcacgg cggctctctt cacggcttct gtcgagcaaa atttcttcga taaaggccag
    27121 atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg gccataatcg cttcaaatat
    27181 gcgggtgcgg tgactactca ccagcggcag catctcgcga cgcgaataga gcaattcgaa
    27241 gttctggcgg acattctggg ccaacatcgg ctccatacac cttagcagat gaagcagaac
    27301 cacattgtgg gccgcttcgg tgacggcaat ctgatactgg agtacggcgt ttgattccgc
    27361 gtccagatcg ccagactgct gcgccagctc tatggcgtgg tggagttcac ggatgcgttc
    27421 cttgtcttca tcggtactac gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg
    27481 tcgtgtttcg agcaagtcat actgtgattc aggatggtcg gagagcagct ccaccagcgg
    27541 atcgctgaag ctttgccata ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag
    27601 caacaagccc ttcgcttcga gacgttgaat cgcctcacgc aaggagggac gggagacgtc
    27661 gaactgtttt gccagttcgc gttccggtgg gagtttttcg cccgggcgga gagtgccttc
    27721 gaggatcaaa aactccagtt gctgctcaat cacatcggag agttttggtt ggcggatttt
    27781 gctgtaggcc atgagttcct gtcttaagcc acttgccgaa gtcaattggt cttaccaatt
    27841 tcatgtctgt gacgctaaag taacaaagta ttcaccttat gtccatacag gttttgattg
    27901 aaatcatgaa actgtgcaca ttttaacaac ttgacatata taacgtttca aagttgtaac
    27961 tatgcacaaa tgtagacttt acgtaggaaa gggatttttt aacgattatt aaactataaa
    28021 aatccgaatt gaaccgattc acttaccaat tttttgattt ttaagtcaat aaaaacgaat
    28081 ttaaattcac tctgcatatt gagaggggtt gaggctgagc tttacaaacg gtttcttttt
    28141 aagcaactca tcttcaacca tgcataaagc gggtgcattc gctgccgcat accattattc
    28201 ttgatctgac ggaagtcttt ttgtaacaat tcaaacttcc ttgatgtaaa taaatcaata
    28261 caacaaacgg aattgcaaac ttacacacgc atcactgcgt agatcaaaaa aacaatcacc
    28321 gcacgaggtt tcatgatgga aggtcaacag cacggcgagc agctaaagcg cggccttaaa
    28381 aaccgccata ttcagcttat cgcgctgggt ggcgcgatag ggacagggct attcctgggt
    28441 agcgcctccg taatacagtc cgcagggcca gggattatcc tgggttacgc cattgctggt
    28501 tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg tggtcgaaga acctgtcgca
    28561 ggctccttta gccactttgc ttataaatac tggggcagtt ttgccggttt cgcctctggc
    28621 tggaactact gggtactgta cgttttagtt gccatggcag agctgactgc cgtgggtaaa
    28681 tacattcagt tctggtatcc ggaaatccca acctgggttt ctgccgccgt attctttgtg
    28741 gtgattaacg ccatcaacct gaccaacgtt aaagtgtttg gcgagatgga gttctggttt
    28801 gccattatca aagttattgc ggtagtagcg atgatcatct tcggcggctg gctgctgttc
    28861 agtggcaacg gcggcccgca ggcgagcgtt agcaacctgt gggatcaagg tggtttcctg
    28921 ccgcacggct tcaccggact ggtgatgatg atggcgatta tcatgttctc gttcggtggt
    28981 ctggaactgg tggggatcac cgcagcagaa gctgataacc cggagcaaag tatcccgaaa
    29041 gcaactaacc aggttatcta ccgcatcctg attttctata ttggttcgtt agccgttctg
    29101 ctctcactga tgccgtggac ccgcgttacc gccgatacca gtccgtttgt gctgatcttc
    29161 cacgagttag gcgatacctt tgtggcgaat gcgctgaaca tcgtggtact gactgcggcg
    29221 ctctccgtgt acaacagctg cgtatattgc aacagccgta tgctgtttgg tctggcacaa
    29281 caggggaacg cgccaaaagc gctggcgtct gtcgataaac gcggcgtacc ggttaacacc
    29341 attctggtgt ctgcgctggt tacagcattg tgcgtattga ttaactatct tgctccggaa
    29401 tccgcatttg gcctgttaat ggcactggtg gtatccgcac tggtgatcaa ctgggcgatg
    29461 atcagtctgg cgcatatgaa gttccgtcgc gccaagcagg aacaaggcgt ggtaactcgc
    29521 ttccctgctc tgctttatcc gctgggtaac tggatctgcc tgctgtttat ggcggcggta
    29581 ctggtgatta tgctgatgac cccaggaatg gcgatttcgg tatacctgat cccggtatgg
    29641 ctgatcgtgt taggtatcgg ctatctgttt aaagagaaaa ccgccaaagc cgtaaaagcg
    29701 cattaatctc tctacgccct cacccgtaca gggtgagggc aataatctct acaccaacgc
    29761 cccgtaaatc gtcagtagcg caatcaccac cacgaccacg aacgaggttt tcttcgccat
    29821 tgaaaccgct gccttcggcg tctccacctt atcgacatgc ggttcacgcg ccagagagaa
    29881 ctgcgccaga cgcgttaata cctgatattg cgaagtatgg aaatcaccca gcgaagcaaa
    29941 ccaggccggt aacgctttct caccatgacc gatcaaggca tataccacac ccgcaagacg
    30001 aaccggcacc caatccagta catgaagcac tgcatcaatg ccagactgta aacgatgatg
    30061 cggcgtctgg tatcgcgcca gccagtattg ccatgcacgc aagaacgcat accccatcag
    30121 cgtaacgggt ccccaggttc cccccacaat cagccagaac agcggtgcaa gataaaaacg
    30181 aaagttaatc cacagcaatg cattttgcag ctcacgcaaa tactcacgtt cgtcgcagcc
    30241 tgccgggacg ccgtgaatca tggtgagttc gccagccatc gtggcacggg catggctatc
    30301 attacgtgaa gcagctgtca gataagcatg ataatgaaga cgaactttac ctgcgccaat
    30361 acacagcaaa ccaatcagca gccacaccag cagcgtggga acgttgaaca atactccctg
    30421 caatgcgcgt aacagtaaaa aagtcacacc catcgcaata atggtcatgc ctaacgtgcg
    30481 cccgagagaa aaatgtttca cccgccgaaa gaacgcttca agacgatgat caagctgcca
    30541 gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc agtaaggttg taaatagcgt
    30601 catgttgtct ccttgctgac cagcgcacga aaccgtgccc aatcaaatgc aggaccggga
    30661 tcggttttcc gatccggcgc aatatcacaa tggcccgtca tgttattagc gatatcggga
    30721 tagcgatcta tcagtgcccg cgtaaccgcc gcaagctgtt gatactgcgc atcggtatac
    30781 gccagagtat cggtgccttc aagctcaatc ccaatagaaa aatcattgca gcgttcacgc
    30841 ccctgatact gagagactcc cgcatgccag gcacgtttat cgaaaggaac atactggact
    30901 atttcaccat cacggcgaat caaacagtga gcggagacgc gcaaatgggc gatctcagca
    30961 aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata atgcgtcgat ccacggaccg
    31021 ccaaactcgc ctggcggcag gctaatattg tgcaccacca gcagggtggg tgtttcgtca
    31081 tccaggcggc aatcgtaatg tggtgaggga acgcggcgcg cgccaaccag ccacccctgt
    31141 tctaacaaca tgcaggatct ccttatatgt ggtgctaata cccggttcag agtagcatgt
    31201 ttctacctta tgattcgtta gctatctgga gttttaacat gccgcctcgc cgctacaacc
    31261 ctgacacccg acgtgacgag ctgctggaac gcattaatct cgatatcccc ggcgcggtgg
    31321 cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc caacaatgac attacggcaa
    31381 aacttttacc ggaaaattct cgctctcatg ccacggtgat cacccgcgag aatggcgtat
    31441 tttgtggcaa acgctgggtt gaagaggtgt ttattcaact ggcaggcgac gatgtcacca
    31501 taatctggca tgtggatgac ggcgatgtca tcaacgccaa tcaacccttg ttcgaacttg
    31561 aaggcccatc ccgcgtgctg ttaacgggcg aacgcactgc gcttaatttt gtgcaaaccc
    31621 tttcaggagt tgccagtaag gtacgccact atgtcgaatt actggaaggc accaacacgc
    31681 agttgttgga tacgcgcaaa accttacctg gcctgcgttc agctctgaaa tacgcggtac
    31741 tttgcggcgg cggagcgaat caccgtctgg ggctttctga tgccttcctg atcaaagaaa
    31801 accatattat tgcctccggc tcagtgcgcc aggcggtcga aaaagcgtcc tggctgcatc
    31861 cggatgcgcc agtagaagtc gaagtagaga atctggaaga acttgatgag gccctgaaag
    31921 ccggagccga tatcatcatg ctggataact tcgaaacaga acagatgcgc gaagccgtca
    31981 aacgcaccaa cgacaaggcg ctactggaag tgtctggcaa cgtcactgac aaaacactgc
    32041 gcgaatttgc cgaaacgggc gtggacttta tctccgtcgg tgcgctaact aaacacgtac
    32101 aagcactcga cctttcaatg cgttttcgct aattgctgac agatttatcc gctccatcgc
    32161 gggcggataa tctccccttc cccaactttc ttcgtaacgc ctcgcaaatt tcttgtcttt
    32221 cgctgcaacg aaataaaaaa cctcggaatc tgctttcccg tttcgttttt tgcccctcgc
    32281 ctgtgctgat ggcgtttgcc aaagtagcac caaccaaatc aaggagcgaa acagatggac
    32341 aagcaacgcg gttttacact tatcgaactg atggtggtta ttggcatcat tgccatttta
    32401 agcgccattg gtattcccgc ttatcaaaac tacctgcgca aagccgcact caccgacatg
    32461 ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt gcgcgctgga acatggtgga
    32521 ttagatacct gcgacggtgg cagcaatggc attccctcgc ctaccaccac ccgctatgtt
    32581 tcagccatga gtgtggcaaa gggcgtggtg tcgctgaccg ggcaagaaag tctcaatggg
    32641 ctaagcgtcg tcatgacgcc aggttgggat aacgcaaacg gcgtcaccgg ctgggcgcgc
    32701 aactgcaata ttcaaagtga cagcgcattg cagcaagcct gcgaagatgt cttccgcttt
    32761 gatgacgcca actaaggagc ggcaatgaat attccacagc tcacggccct gtgcctgcgt
    32821 tatcagggag tcttgctgga tgccagcgaa gaagtggttc atgttgcggt ggtcgatgcc
    32881 ccctcacatg agttgctgga cgcattgcat ttcgctacca ccaaacgtat tgagatcacc
    32941 tgctggacgc gccaacaaat ggaaggtcac gccagtcgca cacaacagac attgcccgta
    33001 gctgttcagg agaagcatca gcccaaagca gagttgctga ctcgaacgtt acaatctgcg
    33061 ctggaacaac gcgcgtctga tattcatatc gaaccagcgg acaatgccta ccgcatccgc
    33121 ttgcgtatcg acggcgtatt gcatccttta ccggatgttt caccggatgc cggagtcgca
    33181 ttaacagcca gattaaaagt gctgggaaac ctggatattg cggaacatcg cctgccgcag
    33241 gacgggcaat tcactgtcga actggcagga aacaccgtct catttcgtat tgcgacctta
    33301 ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac agcaggtgag ccaggcactg
    33361 gatgttaaca cgctgggaat gcagccgtta caactggcgg actttgctca tgccttgcaa
    33421 caaccacagg gactggtgct ggtaactggc cctacaggca gcggcaaaac ggtcacgctt
    33481 tatagtgccc tgcaaacgct gaataccgct gacattaata tttgtagcgt cgaagatccg
    33541 gttgagatcc ccatagccgg actaaaccag acgcaaatcc atccgcgtgc cgggctcacc
    33601 tttcagggtg ttttgcgtgc gttattgcgc caggatcctg acgtcatcat gatcggagag
    33661 atccgcgatg gcgaaacggc agaaattgcc attaaagccg tgcaaaccgg tcacctggtg
    33721 ttgtccacgc tacacaccaa ttccaccact gaaacgctgg tacgtttaca gcaaatgggg
    33781 gttgcccgct ggatgctatc atcggcgctt acgctggtaa tagcccagcg tctggtacgt
    33841 aaactttgcc cacattgtcg ccgacagcaa ggggagccca tccatattcc agacactgta
    33901 tggccgtcgc cgctgcccca ctggcaggca cccggttgtg tacattgcta ccacggtttt
    33961 tatggtcgta ccgccttgtt tgaagtcctg cccattacac cagccatacg ccagcttatt
    34021 tccgctaata ctgaagttga atcgctggaa acgcacgccc gacaggcggg tatgcgtacg
    34081 ctttttgaaa acggctgcct ggccgtggag caaggcttaa ccacctttga agagttaatc
    34141 cgcgtactgg ggatgccgca tggcaagtaa gcaactctgg cgctggcatg gcataaccgg
    34201 cgacggcaat gcgcaagatg ggatgctatg ggcagagagc cgtactttac tgcttatggc
    34261 actacagcaa cagatggtta ccccactaag cctgaagcga atcgccatca attctgcgca
    34321 gtggcgagga gataaaagcg cggaagtcat tcatcaactg gcgacgctac ttaaagccgg
    34381 gttaacgctt tctgaagggc tggctctgct ggcggaacag catcccagta aacaatggca
    34441 agcgttgctg caatcgctgg cgcacgatct cgaacagggc attgcttttt ccaatgcctt
    34501 attaccctgg tcagaggcat ttccgccgct ctatcaggcg atgatccgca cgggtgaact
    34561 gaccggtaag ctggatgaat gctgctttga actggcgcgt cagcaaaaat cccagcgtca
    34621 gttgaccgac aaagtgaaat cagcgttgcg ttatcccatc atcattttag cgatggcaat
    34681 catggtggtt gtggcaatgc tgcattttgt tctgccggag tttgccgcta tctataagac
    34741 cttcaacacc ccactaccgg cactaacgca ggggatcatg acgctggcag actttagtgg
    34801 cgaatggggc tggctgctgg tgttgttcgg ctttctgctg gcgatagcca ataagttgct
    34861 gatgcgccgc ccgacctggc ttatcgcgcg gcagaaattg ctgttacgca tcccgattat
    34921 gggttcactg atgaggggac aaaaactcac gcagatcttt acgattctgg cgctgacaca
    34981 aagtgcaggc attacttttt tacagggcgt agagagcgtc agagaaacaa tgcgctgccc
    35041 gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc atcccatctg
    35101 gctggcgcta aaaaatgccg gtgagtttag cccgctctgt ttgcaattag tgagaacagg
    35161 agaggcatcc ggctcgctgg acctcatgtt agacaacctc gcccatcatc atcgggataa
    35221 cacaatggcg ctggcggata acctcgcagc cttactggaa ccggcgttgc tgatcataac
    35281 gggaggaatt atcggtacgc tggtggtggc aatgtatctg ccaattttcc atttaggcga
    35341 tgcgatgagt gggatgggat aacgctggcg tgttgctcca cgccagcgtt gggagattac
    35401 aggttgttga agatgcggtt ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc
    35461 agctctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc gcccaaaata
    35521 tcacgcgcgg tattttcaac cgggcctcgc agcggcagct tgacggtttt accttctgct
    35581 gcgcggtatt ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga gctcatgccg
    35641 tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg
    35701 cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca
    35761 tcgcccggcg tggtacagcc accgtcgctg acgatcattc cgcccagacc gtgcgccgca
    35821 tcggcacatt cgattaccgc agaaagctgc ggataaccga cgcctgtttt gacgcgagtt
    35881 gtacaaacag aacctgggcc aatgccaact ttaacgatat cagcaccgga gaggataagc
    35941 tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt cggccacgct
    36001 tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc cacatcaata
    36061 caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt tagttttttc gaaatccgca
    36121 tcagacgtgc cggtagaaac catcacatgt ttcagcacat cagcggaaga attgttgata
    36181 aacgcttgcc actcttcgac agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa
    36241 gccagcgcag aggccataga aaatgtgcct acggtgtcca tatttgcggc gataatcggc
    36301 acgccggacc agctctgacc tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg
    36361 gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa acctaacttc
    36421 agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag ggtaagagcg
    36481 caactccagt gacgctatca tacgcactaa tcaatgcggc gcaagactgc gaaattgtct
    36541 ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg tttcatcaat
    36601 ttcactttcg cgcgctcatt tttaacgttt tgataatcat gatgaattcc cgggaatata
    36661 aattatgggg tatatagttg ccttaacggg aggcattggc agtggtaaga gtaccgttgc
    36721 caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg cgcgtcaggt
    36781 ggttgaacca ggtgcacctg cgctacatgc cattgctgat cactttggcg aaaacatgat
    36841 tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg ccaacccgga
    36901 agagaaaaac tggcttaacg acctgctgca tccgctgatt cagcaagaga cgcaacacca
    36961 gatccagcaa gcaacttccc cctatgtact gtgggttgtg ccattgctga tagaaaactc
    37021 actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa
    37081 gcgcaccatg cagcgcgatg atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca
    37141 ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc attgataata acggcgcacc
    37201 ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca
    37261 gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca tccactaaat
    37321 gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac cgttaattta
    37381 cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgtcagtga attactggat
    37441 gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt
    37501 aaactccaga cctggattgg cgtgcctggc gtggaccaga gccgtattga agcattaatt
    37561 cagcagttaa aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg
    37621 cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccagg cggctgttgc
    37681 agctttgatt tacctacatt gcacatttgg ctgcatctac cccaggcgca gcgcgacagc
    37741 caggtagaaa cctggattgc cagcctgaac ccgctcaccc aggcacttac catggtgctg
    37801 gatttaattc gccagtcggc ccccttccgt aaacaaacca gcctgaatgg tttttatcag
    37861 gataacggtg gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc acagctttat
    37921 ccgcaaattt ccggacataa gagccgtttt gccattcgtt ttatgccgct ggacactgaa
    37981 aacggacagg taccggaacg tctggatttc gaactggcct gttgctaagg agtaaaaatg
    38041 tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc
    38101 agcccgtttc ggccattttg ctccaaacgt tgtcagctga tcgacctcgg agaatgggct
    38161 gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga ctggagcgaa
    38221 gagccaaagc agtgacattt gtctgatgcc tcacgtaggc ctgataagac gcggacagcg
    38281 tcgcatcagg catcttgtgc aaatgtcgga tgcggcgtga acgccttatc cgacctacag
    38341 acgcttaagc ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg cattaagacc
    38401 gaccagcgac atccactcac cgggttgccc ttctttaccc cacggcaccc cttcccaact
    38461 ttcgaccagc cagaaccaca gagttatatg cctgtccggg aattcatatt ccagtttttc
    38521 aaatagcgaa aaatgttgtg gggtaatccc gacctcttcc tgaagttcac gcaccaccgc
    38581 ctgttccggc gtttcgccca tttcaatttt accgccggga aactccagtt tattcgccat
    38641 gtgcgcatct gctgcgcgac gtgttataaa gatttcattg ttctcgttgc gaataatacc
    38701 taccgcaatt tgcagctttt tcattgtctt aaacctataa aaaaggcgca gaatcctgcg
    38761 ccttttactt caactgttag cttttattgc aggcgaccat ggcactgctt gtatttttta
    38821 ccagaaccgc acgggcaagg atcgttacgt cctactttgc gttcaccggt ttgcgccgcc
    38881 agtgcagctg cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa
    38941 cgctcggctt ccatacgacg ctgttgttcc agctcctcaa cctcttcagg catacgtacc
    39001 tgaactttgc tcagcgtact gataacttca tatttcaacg actccagcat cgctgcaaac
    39061 atggagaacg attcacgttt gtattcctgc ttcggatctt tctgtgcata gccacgcagg
    39121 tggataccct gacgcagata gtccatcgct gccaggtgct ctttccacag ggagtcaagc
    39181 gtttgcagca tgacgccttt ctcgaagtga cgcatcatct cagcaccaac cacttcttct
    39241 ttacgctgat acacttcgat ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc
    39301 agttctggtt ctttatccag ccactcggca attggtaaat cgaggtcgaa atcgttcttc
    39361 agacgttcct gcagccccgg aatatcccac atttcttcca gcgactgtgg cggaatgtag
    39421 gcatcaatgg tggctttgaa cacatcttca cgaatgctgt tgatggtttc gctcacatcg
    39481 ctgacatcca gcagttcgtt acgctgggag taaatggcgc gacgctgatc gttagccacg
    39541 tcatcatatt ccagcagttg cttacgaatg tcgaagttac gactttcaac tttacgctgg
    39601 gcgttggcaa tcgctttagt cacccacggg tgttcaatgg cttcgcctag cttcataccc
    39661 agtttacgca tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc
    39721 atcgacaggt agaaacggga agaaccagca tccccctgac gaccagaacg accgcgcaac
    39781 tggttatcga tacgacggga ttcgtgacgc tcggtaccga tgatatgcag gccacctgct
    39841 tccagtaccg catcgtgacg tacctgccag tcggctttaa ttttttcaat ttgctctgcg
    39901 gtcggatttt ccagcgcggc aacttctgcc tgccagctac ctccgagcac aatatctgta
    39961 ccacgacccg ccatgttggt cgcgatagtc accgcagccg gataacctgc ctgagcaaca
    40021 atcgccgctt cgttggcgtg gaatttggcg ttcaggacat tgtgcttaat accggctttg
    40081 gtcagttcgt ttgacaccag ctccgatttt tcgatggaga tagtacccac cagcaccggc
    40141 tggcctttcg cagtacgttc tttgatatct tcaatgatcg cctgaatttt ttccgcttca
    40201 gtcatgtaga ccaggtccgg cagatcttta cgaatcattg gacggttggt cggaacaacg
    40261 acggtatcca gtttgtagat ggagctaaat tcgaaagctt cggtatcagc agtaccggtc
    40321 atccccgcca gtttttcata cagacggaag tagttctgga aggtgatcga agccagcgtt
    40381 tggttttcgt tctggatctg cacaccttct ttcgcttcca cagcctggtg cagaccatcg
    40441 gaccagcgtc ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat aacttcacca
    40501 tctttaacga tgtagtcgac gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt
    40561 acgtggtgca tcagcatgat gttggccgga gagtacagag actccccttc atccatgatg
    40621 ccctctttca ccagcagttc ttcaatcagc accagaccac gttcggtcag gttcacctgg
    40681 cgagattttt cgtccaccga gaagtggcct tcgccctgga aggtttcgga gtcttctttt
    40741 tcctgacgga tcaggtgcgg aataatttta ttcacgcgtt tatacatttc cgagctgtct
    40801 tctgccgggc cggaaatgat cagcggtgta cgcgcttcat cgatcaggat ggagtccact
    40861 tcgtccacca gcgcatagtg cagtttacgc tgtacacgtt cttcagggct gaacgccatg
    40921 ttgtcgcgca ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg
    40981 taagcttcgc gctttgccgg tgctggcatg cccggcaggt tgataccaac agtcaggcca
    41041 aggaattcaa acagcggacg gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg
    41101 gtaactacgt gcacgccttt accggttagt gcgttcaggt aagcaggcag cgttgcggtc
    41161 agggttttac cttcaccggt acgcatttcg gcgatgcagc gttcgttaag aaccataccg
    41221 ccgagtaact gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc
    41281 acggcgaaag cttccgggat cagattttcc agcacttcgc ctttttccag acgcgcacga
    41341 aactctgcgg ttttcccttt cagttcttca tcggagagtt tttccatctc cggttccatg
    41401 gcattgatga tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg
    41461 aaaactttag ttaacaattt gattagcata ataaaatctc aaacgccccg cgttacggag
    41521 ttaataaaat gaagtaaagg tttattgttg ttaggtgagg cgttgagggc cagcacggat
    41581 gccttgcgcc tggcttatcc agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc
    41641 ataatcaatg cgataaccct tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag
    41701 cgtatccagt aatgcaagat gttgcgcctg aagaggcaaa gattcttcag caacgggcag
    41761 tgtttgcggt gccattgcga aagaaagatg acggattacc gtgcgaatag catgttgatg
    41821 ccagtaatca acggaatagt tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa
    41881 ttgaccaaag ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc
    41941 gtttggttcg gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa
    42001 taagagatgc ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca gtattccact
    42061 cacgttattg ccatcccgat cgaattcaaa cgtatttagc gcacaaatct tatcattaaa
    42121 gcctgaagtc cacagcacga atgaagataa gttgtgcgta aaagtgctta agaaaacagc
    42181 gttcgcaccg ttttttatag ctcttataaa attcaaaaaa aaacgactgg ttcacctggc
    42241 cggagagagt gccagatttg ccagtcgaat tttatacgac agtataaatg tcgttatgcc
    42301 agtacagctg aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca
    42361 tattcccagg cttcctgttt cgccaggaca gcctgcagca gtttgttatt cagtgcatga
    42421 ccggatttat aagcggtaaa tgcaccaata atattgtgac cacacatgaa caagtcaccg
    42481 atcgcatcga gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc
    42541 agtacgcgat aatcgtcaac aacgatggca caatcgaagc tgccgcccag gcacaaacca
    42601 cgggactgca gatattcgat atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg
    42661 cgcataaacg catcagcgga gaagttcatc gcatagcgct ggttgctgga atcaatagcc
    42721 ggatggttaa aatcgatggt gaaatccagc gaaaaaccat tgtacggctt aaattcagcc
    42781 cacttatcgc catcttcgac acgaacagtc tctttgatgc gaacaaattt tttggcgcag
    42841 ttcaactcgt cgataccggc gtcaagcagc aggtatacaa acggagcggc gctgccgtcc
    42901 atgatcggga tttccggcgc gttaacttcg ataacaatgt tatcgatgcc caggcccgcg
    42961 agagcagcat tgaggtgctc tacggttgaa atccgtacat catgctcgtt gaccagacac
    43021 gtacagagca tggtatcacg cacagatttg gcatcggccg ggaaatctac cggtggattc
    43081 aagtcggtgc gacgatagat gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg
    43141 actttcttgc cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt
    43201 tgtttgatca tcgtattatc tcgccaaatt acctatccaa ccgaagtgta ctatacattc
    43261 ggcgggccag tttagcacaa agagcctcga aacccaaatt ccagtcaatt cttaatcagc
    43321 ttgcttacgt aggaatgctg ggatatccag ataatccggc tctttcgcag tttgcggcgc
    43381 attgtcattc actactttag caaccggctt ctgctcctgg gtcagcggag ccatcccatg
    43441 ctgctggtag cgatccatca ctggctgctg aacctgctta ttggtcacca gagtgatttc
    43501 aggacgtttg tccatgccga tacctgtcgc aacaacggtt acgcgcagct cgtcattcat
    43561 atccgggtca agagaagtac cgataaccac agtcgcgttg tcggaagcaa atgcacggat
    43621 ggtgttacct accgtttcga actcatccag acgcaggtcg aagcccgccg tgatgttaac
    43681 cagcacgccg cgcgcgccag acaggtcgat atcttccagc agcggagaag agatagccat
    43741 ttcagcagct tcttccgcac ggtcttcacc gctcgccacg ccagaaccca tcattgcgta
    43801 gcccatttca gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg
    43861 agtaatcagt tcggcgatac cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa
    43921 cgcatccagc agggagatac cgcggcccag aactttcagc agtttgtcgt tcgggatagt
    43981 gatcagagag tccacatgct tggacagttc agtgatcccc tgctcggcga atgccatacg
    44041 cttcttgcct tcaaagttga aaggcttagt gacgacagca acggtcagga tacccaaatc
    44101 ttttgccact tcagcgacga ctggtgctgc acctgtaccg gtaccaccac ccatacccgc
    44161 agcaataaag accatgtctg caccttccag cgccgcacgc aatgcatcgc gatcctcatc
    44221 agccgcattg cggccaactt ctggattagc gccagcgccc agtcctttgg tgataccgct
    44281 accgatttga atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat cggtatttac
    44341 cgcgaagaat tcaacacctt caatacgctc gcgcaccatg tgttcaacag cattaccgcc
    44401 gccgccgccg acgccgatga ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa
    44461 catagtttct ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa
    44521 aaattaaaac tcttttcgca gccaactatt gagtcgcttg atccacgagc caactgatgc
    44581 tgtaacacgt ttttctactt cagcttcacc gttaagatgt gactctttcc catagtgaag
    44641 caatcccacc gccgtcgaat aatacggctc ctgagcataa tccgttaaac cggtaatgtt
    44701 cagcggcgcg ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc
    44761 ttcgatctgc gctgcgccac cggttaatac aatgcctgcc gccaggtgat gtttaacccc
    44821 ttgttggcga agcttttcct gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc
    44881 ggtatagcgc ggctcgatca cctctgccag ggtctgacgt tgcagactcc gcggcggacg
    44941 accgcctacg ctcggcactt ccacgctctc atcttttcca acgatggaac ccagcgcaca
    45001 accgtggcga actttaatcg cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat
    45061 atcactggtc acgacattgc cagcataagg aattacctta gtgtggcgta atgccccacc
    45121 ggtataaacg gcgatatcca ttgtaccacc accgatatcg acgacgcaga cacccagttc
    45181 acgttcatct tccgtcaata ccgaataact tgatgccagt ccggcaaata tcagttggtc
    45241 aactttcagc ccacaacgtt caaccgcttt gacgatgttt ttcgccatat cgttgtgaca
    45301 tgtgatcagg tgcacttttg cctgcatccg cacacccgaa agtcctaccg gattcttgat
    45361 cccttcctga tagtcaatcg catactcttg cgggatcaca tgcagcacac gatgctcatc
    45421 gcgcacacgc accgatttcg cggtatggac gacgttttcc acatcttctt gcgtcacttc
    45481 ttcttcagaa ataggcacca taccaatttc attctggcag ctgatgtgct taccagaaag
    45541 cgccagatat accgaagaga tctgacaatc tgccatcaat tctgcctggt caatggcgcg
    45601 ttgtacgcac ttgaccacgg attcgaggtc gttcaccccg cctttatcca taccacgtga
    45661 cgggcagctg cccacgccaa tgatattgac cataccgtcg ggcagaactt cccctactaa
    45721 agcggcaacc ttcgcggtac caatttccag tcctactacc agttttctgt ccgtcgcctt
    45781 gatcattgtt gttctgcctg tgcctgattt tgttgctgag tagattcctc tggcggcaag
    45841 ggcgcccagc ctactgccgc tccagagtca taacgcaaat caacgtagct aatccgtttg
    45901 ccatcggttt gcgcctgctg ctgtaaaacc ggataaagtt ctacaaagcg agccaaacgt
    45961 ttcatcgtat cgccccggcc aagattgagc ttaatatcgt tattcagcgt caactgccag
    46021 gaacgccgcg cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc cagcatctgc
    46081 cccatttcgc gatagccctg caacacttca ttagcgctgc cttccgggcc atacagcatt
    46141 ggaagcacct gcttgctggt gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct
    46201 accatgtgtt gatcattcca ccgcgcaatc ggcacatatt caaccagatg aatcttcaat
    46261 tcatcaggcc actgctttct gacgctcacc tgcttaatcc acggcaggcg ttgttctatt
    46321 tgcgtctgga tgatgttgac atcctgggtc ataaaggtac ccggctcacc caatgccagg
    46381 atcgactgcc ggatatcgtc attacgcgtg taatggcgtt caccggtcaa caccagcttt
    46441 gagagcggca ggcgttgcgc atcttccatc cagctcaaca cgacccagcc gctcaccaac
    46501 actgtcgtta aaacggtcag caggaaaagg atccccgcca gacgcgttcc attattgcgg
    46561 cgagaagaaa cctcttcttc gctgtttcgc gtgttcagag cagcctgcga catattagtc
    46621 cgccagttcc agaattcgta ctaccaactg cgagaagctc atacctgcct gacgtgccgc
    46681 catcggcacc aggctgtggc tggtcatacc cggtgaggta ttggcttcca gcagataaaa
    46741 ctgtccatca ctgtccagca taacgtcaat acgtccccat cctttgcaac ctaacgtcgt
    46801 ccatgctttc agcactaatg cctgcaaatt ggcctcttgt gacgctttca gacctgcggg
    46861 gcagaaatac tgtgtctcat cagagagata cttcgcctca taatcataga aggttccgga
    46921 cggttgaata cgtattgacg gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg
    46981 cccacttagc catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc
    47041 atcttgtaga gcattttctg ctactacttt tgacattccc acactggaac cttcgcggct
    47101 cggcttaacg ataaccggca aacccagagc agaaatttct gctaactgct tatcgctcag
    47161 gcctttttca aactctgcgc gggttaacgc tacccacggc gcgaccggta aaccggcacc
    47221 ttgccacaga agtttgctgc gtagtttatc tattgaaagc gcagatgcca tcactccgct
    47281 gccggtataa ggcaagccca tcagctcgag catgccctgc agcgtaccat cttcaccgcc
    47341 gcgaccgtgt agcgcgataa acactttctg aaaacccatc gacttcagtt gcgtcacgtc
    47401 gacttctttc gggtcgacag gatacgcatc aataccgcct tcacgcagcc cggctaacac
    47461 cgctgcgcca gaattcagag aaacttcccg ctcagcggaa gtcccgccca acaggaccgc
    47521 gattttatca gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg atttcagcta
    47581 aagaacgggc aatttttccg atattaccag ccccctgaac gagaatcagg tcgttaccgg
    47641 ttaataccgg tgccagcatc tcggctacct gcgcagggtc cggcaccaga atgggatcaa
    47701 ttttcccacg tccacgaatt gtgcgacaca gcgaacggct gtccgctccc ggaattggcg
    47761 cttcgccagc cggatacact tccagcatca acagggtatc aacctgcgtc agcacattgg
    47821 cgaaatcatc atacaggtcg cgcgtacggg taaaacggtg cggctgaaac agcattacca
    47881 ggtttttatc cggccagcct gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt
    47941 ggtggccgta gtcatcgacc agcattgccg taccgctttt accattcact ggctccagcg
    48001 ggaattcacc gaggaaatca aaacggcgac cagtcccctg gaagctctcc agcgcacgca
    48061 aaatagcctc gtcgtcaatt ccctcttccg tagcaaccgc aaccgcagct gcggcgttca
    48121 gcgcgttatg acggcctggc gcattcaggg tgacgcgcat cggctctttg tcctgacgca
    48181 gcagcgtaaa gtgcccctgc gggccaatct gctgataatc ttctacacgc acgtcggcat
    48241 cttcgctgaa gccgtaagtt gtggtctgac gtcccacacg cggtaacaat tcgcggatca
    48301 ccggatcatc aacacacatc accgcacgac cgtaaaaagg caggttgtgc agaaaattaa
    48361 taaaggtctg ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga
    48421 tattggtgac aatcgccacc atcggttgca gatgcaggaa cgatgcatca ctctcatctg
    48481 cttcggcaat caggtaccga ccatgcccca aacgcgcatg aacccccgcc gcttttacca
    48541 gcccgccgtt aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg
    48601 cggtagttgt cgttttgccg tgcgttcctg caatggcgat gccatgacga aaacgcatta
    48661 actcagccag catttcggca cgacggatca ccggaatacg cgcttcatga gctgcgacaa
    48721 tttccgggtt gtcggcagaa atggcgctgg aaacaacgac cacgctggca tcacgtacgt
    48781 tttccgggcg atggttgaaa taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg
    48841 gatttggcgc taaatcggaa ccactgatct gataaccttc attggccaga acttcggcaa
    48901 taccgcccat accggcacca ccaatgccga caaaatgtat gtgccgaacg cgacgcattt
    48961 cgggcacgat ggaacgcagt tttgccaatt gttgtgtatt cattctttac gccattaact
    49021 tcttaaattc atacgatgca aaaggcatcg ctacaattac gcccgggcag cccggctcac
    49081 ttcatttgcc actcgctcgg tggcatccgg aatggatgca gcgcgggcgc gttctgccat
    49141 ggttaataag gtttctcgcg accacccggc cagggtgttg gcgacagcat ccacgctaag
    49201 ctgtggctgc tcgataattt tggctgcgcc cgctttttcc agcggtagcg cattccagta
    49261 ttgctggcgg tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat
    49321 ttcactcaca gttaaagcac cggagcggca aacgacgaca tccgcccacg catacgccgc
    49381 cgccatatca tcaataaatt cagtcacttt atgctgcggt tgccccgctt cggcatacgc
    49441 ctgttcaacg gattgttgcg aacctttgcc gctctgatgc cagatagtga ctgaatcacc
    49501 cagtttcgca gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct gggaaccacc
    49561 cactaccaac acacgaaccg gaccttcacg tccagccaaa cgttgctgcg gcaacggcag
    49621 cgccagcaca tcggtacgca ccgggttacc caccacttcc gcattaggga aagcgcctgg
    49681 aaacgcctgc atcactttgg tagcaatctt cgccagccat ttattggtta agcccgcaat
    49741 accgttttgt tcatgaagta caaccggaat gcctaacgac cacgcggcca gaccacctgg
    49801 acctgataca tagccgccca taccgagtac cacgtcaggt ttgtacgctt tcataatcgc
    49861 ccgcgcctga cgccagccgt tgaagatacg cagcggcgca gctatcagtg cttttatacc
    49921 ttttccacgc aaaccagaga tacgaatgaa atcaatttcg atgccatgtt ttggcactaa
    49981 gtccgcttcc atacggtcgg cagtccccag ccagcgaact tgccaaccct gagccattag
    50041 atagtgcgca accgccagtc ccgggaatac atgtccaccg gttccgcctg ccatcaccat
    50101 taatcgcttt ccttgaccac tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga
    50161 cgcgtttcat aatcaatacg caacagcatc atgatggctg tcgacataat cagtaagctc
    50221 gaaccaccgt aactgattag cggcaatgtc agacctttgg tcggtaacat ccccgccgcc
    50281 gcgcctacgt taaccagcgc ctggaagcta aaccagatgc caatagaaca ggcgagaaaa
    50341 ccggaaaaac ggtggtcaat ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg
    50401 acgaagaata ccattaaaag tgctagtacc acaccgacat aacctaattc ttcgccgata
    50461 atggcgaaaa taaagtcagt gtgcgcttcc ggcagatact ccagtttttg taccgaatta
    50521 cctaaacctt gcccccaaag ttcgccgcga ccaaacgcca tcagcgattg cgttaactga
    50581 tagccgctgc caaagggatc ttcccacggg ttccagaatg cggtaacacg gcggatacgg
    50641 tacggttcgg cgaggatcag caacacaacc gctgaaatgc ccataccgat aatggcaatg
    50701 aactgccaca atttcgctcc cgccaggaac aacatcgcca gcgtagtcac aaacaacacc
    50761 accaccgtac caaggtctgg ctgtgccagc agtaacactg ccaacaccag aatcacgccc
    50821 atcggtttca ggaagccgcg caggttatta cgtacttcgt cgcctttacg caccagatag
    50881 ttggcgatat agcaaaacag cgacagtttt gtcagctccg caggctggat acgcagcaaa
    50941 ccgagatcga tccaacgcga tgccccttta acagcgctac ctactaccag cacgatcatc
    51001 agcaggatga tagatccgag cagcatcgtg gcactgtagc gttgccagaa ctccatcggc
    51061 agacgcagcg taatgatcgc cagaataaac gccaaaatca gatagacacc atcacgcttc
    51121 gcgaagaaga acggatcgtt ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc
    51181 atgataaagc caatcgccgc gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg
    51241 atcaggctgt cggtatcttt ttcccgcgag cccatcaccc agccctttag cgccgtggag
    51301 atccagacca agatactgaa tcctggcagg cgcggcattt tcaggcgagg gagagataaa
    51361 cgcatcaacc taactccttc gccagacggg caaactcatt gcctcgttgt tcaaagttct
    51421 tgaactgatc aaggctggca caggccgggg agagcagaac catatcgccc ggctgaacac
    51481 gcggagccag caagcgcata gcctgttcca tagtttcggt ttgttctgcc acttccgggc
    51541 gtagcgccgc cagctgcgcg ccgtcacgac cgaaacaata cagacgtacg ttatcgccat
    51601 tcaggtaacg cgccagtggg ctaaagtccg ccgatttacc atcgccgccc agcaacaaat
    51661 gcagcgtgcc gtctacgtgc aggccattca gcgccgcttc cgtactgccg acgttggtcg
    51721 cttttgaatc gttaatccag cgcacaccgt tatgctccag caccacttca aagcgatgcg
    51781 gcagaccggt gaaggtggtt aacgctttca ggctgctggc gcgcggtaac cctgcggcat
    51841 ctgccagcgc cagcgccgcc agcgcattgg tgtagttatg ctgcccggaa agtttcatct
    51901 ctttcacatt cagcactttc tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat
    51961 tcaggtgata gtcacccatg ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa
    52021 tcggcattgt taaggcatca tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc
    52081 gcagttttgc tgcacgatac tgttgtaaac caaacgggta gcgatccata tgatcttcag
    52141 tcacgttcag aatggtcgct gctaccgcct gtaagctgga ggtggtttcc agctggaagc
    52201 tcgacagttc cagcacgtac agttcacact catcatccag tagcatcaac gcaggcaggc
    52261 caatattgcc acccacacca acgttaaccc ccgccgcttt cgccatttca cccactagcg
    52321 tggtgaccgt gcttttgccg ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc
    52381 gacaaaacag ctcgatatcg ccaacgattt cgattccggc atcagcggca gcgcttaagg
    52441 atggatgcgc cagtgcaata ccgggactgg cgacaatcag atctgccgcc atcaaccatt
    52501 catcattcag actgcccgtg tggcgttcta cggcttcggg taatttatcc aggctaggcg
    52561 gtgtcatacg cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc
    52621 aggaaagccc ggtgaggccc aggccgataa tgacaacatt tttaccctgg taatcagcca
    52681 tgattaacgt accttcagcg ttgccagacc aatcaggacc agcatcagcg aaataatcca
    52741 gaaacgcaca atgacgcgcg gttccggcca gcctttcagt tcatagtggt gatgaatcgg
    52801 tgccatgcgg aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca ggatgacaga
    52861 aagcgtttct accacgaaca cgccccccat aatcaccagc aggaattcct gacgtagcag
    52921 tacggcgata atgcctaacg caccacctaa cgccagcgaa ccgacatcgc ccataaagac
    52981 ctgcgccgga taggtgttaa accacaggaa gcccagtcct gccccgacta tcgcggtaca
    53041 gacaataacc agttccccgg cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt
    53101 catgttgccg gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat
    53161 aattgccaga ccgtcaagac catcggtcag gtttaccgcg ttgccagtac ccacaatgac
    53221 gaagtaagcc agcagaatgt agaacagccc cagttgcggc atcacatctt taaagaatgg
    53281 gaccaccagc tgcgttgcgg gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc
    53341 cagcgcaatg accgacatcc agaaatattt ccaacgagcg atcaaccctt tggtatcttt
    53401 acgcaccact ttgcgataat catcaacaaa gccaataaca ccgtaaccta ccagcaccac
    53461 caacacgcac cagacgtacg gattggacgg gtaagcccac agcagcacgg agatcacaat
    53521 cgctgtcagg atcataatcc cgcccatggt cggcgtaccg cgcttgctga agtgtgattc
    53581 aggaccgtcg ttacgcacta cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg
    53641 cgggcccatc cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt
    53701 cagataggaa aagacgttaa agccggaata atatttgacc aaatgttcgg ccagccaaac
    53761 taacatgtcc cattctcctg taaagcgcgt actacctctt ccatggcagc actacgtgaa
    53821 cccttaacta aaatcgtaat tacctgttgc tcagcaatca gtgatttaag acgcgtaatt
    53881 aacgcagttt tatcagcaaa atgttcgcca acgccgctgg cggtgctgat agcatggctt
    53941 tgtttaccca tgcttaacac gcggtcaata ccagcagctt ttgccgcctc gcccacctgt
    54001 acatggcagt cttcgctttc agcgcccagt tccgccatat cgcccaccac cagcacgcgg
    54061 tagcccggca tttcagccag tacctgaaca gctgcggtca ttgaaccgac attggcgttg
    54121 taggagtcgt cgagcagcaa ctggttttct gccagttgga tggggaacag acggccagga
    54181 acagctttca gatttgccag ccccgctttg atagcatcaa gcgttgcgcc cacggacatg
    54241 gagagcgcag cggctgccag cgcattcgca atattgtgac gccccggcaa cggcagcaga
    54301 acatcgacgc taccggttgg ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg
    54361 atattggtgg cggtgaaatc gctgttggcg gcattgggtg agaaacgcca cactttgcgt
    54421 gagccaatta cgctctgcca gttcagccag tcgttgttgt cggcgttcat aatggcgata
    54481 ccgttttccg gcaggccgct aaagatttca cctttcgctt tcgcgacacc cgcaagcgag
    54541 ccaaaacctt ccagatgcgc cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc
    54601 agactcacag tcctggcaat ctcgccctga tggttcgcgc caagttcaat aactgcgtaa
    54661 tcgtattccg gcgttaagcg caacagcgtc atcggtacac cgatgtcgtt gttgagattg
    54721 cctgccgtat aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg
    54781 gaggttttgc cggaggaccc cgtcagagca accacgcgcg ccggaacttg ctggcgaacc
    54841 catgcagcca gttcaccaaa cgccagacgc gtatccttga cgattaactg cggcaggtcg
    54901 atgtccagcg gacggctaac cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca
    54961 aaatcgtggg catcaaaacg ttcacctttc agggcaacaa acaggcagcc cggcgtcagt
    55021 tttcgggtat cagtggttac agcatcaagg gtgatatctg caccttgcag ttcaccgttg
    55081 agaatatcgg taagttggct aagggttacg ctaatcatgc aatccccccc agcagacgcg
    55141 ccaccgtgac gcgatcggag tagtccagac gctgattgcc aacaatctgg taatcctcat
    55201 ggcctttgcc cgcgaccagt accacatcat tctctttagc ctgcataacg gcgcaagtca
    55261 ccgcttcagc acggccttcc atcactttgg catgtccggc atctaacatt cccgccagaa
    55321 tatcgttgat gatggcacgc ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca
    55381 cgtcagcaaa ctcttcggca attgcgccca tcagtggacg cttaccttta tcgcgatcgc
    55441 caccacagcc aaagacgcac cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg
    55501 ctttttccag tgcatccggc gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg
    55561 cagtgaacac ttccatacgt ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat
    55621 cagccagtgg atagcccagc gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt
    55681 taaaagcgcc catcagatgg ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa
    55741 tggtcgcgcc gctgtcgtga tagttcactt cggtcgcttt caaccagcgt ccgtgacagt
    55801 tcggattgat atgatcttcc attgataccg caaccgcgtc cggcagtttt gccagccagc
    55861 ggcggcccac ttcatcgtcg gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa
    55921 gcagccattt cgcagcttcg tagtgttcca tatcaccatg ataatcaagg tgatcgcggc
    55981 ttaagttggt aaagaccgac gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc
    56041 cgtgggagga aacttccatt gcgcaaaaag tcgcgccctg atccaccagc cccgccagct
    56101 catgctgaac atcaactgcc gaaccggtag tattttctgt cgggatcact ttccccagca
    56161 gaccgttacc aacggtgccc attaccgcgc tggtttcgcc aagcagttgg ctccactgcg
    56221 ccagaagctg ggtcgtcgtg gttttgccgt tggtgcccgt tacgcctacg agacgtaaat
    56281 tgtcagaggg ttcatggtaa aagcggcccg ccagtgcaga taaacgctcg ttgagctggc
    56341 tgagatagat gaccggtacg ccgtgcattt cacggatttc accgtcggtc gcctcatctt
    56401 tcgcctctgc aataatggca gccacacctt gcgctatcgc ctgcgggata tatcgacgcc
    56461 cgtccgcctg atgacctact acagctacaa agagatcgcc cgccgcagcc acacggctgt
    56521 cgagtgtcat ctctcgcagt gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt
    56581 cgcgcaaatt acgatctgcc acctgtcccc tcgccttgat taatcacaaa ttcattttta
    56641 tcgcccgttg tcagcgcatc cggctcgatg ttcatggtac gcaatacgcc gcccatgatg
    56701 gcaccaaaga ccggcgcgga aacggcgccg ccgtagtatt tacccgcctg cggatcgttg
    56761 ataacaacaa ccagcgcgaa gcgcggctga ctcgcaggcg caacgcctgc ggtataagca
    56821 atatatttat tgatgtagcg accgtccggc ccgacctttt tcgcggtacc ggttttaatg
    56881 gcgatacgat agcctttaat cgccgccttc acgccgccgc cgcctggtag cgccacgctt
    56941 tccatcatat gcaccacggt gcgaacaatg gattccggga agacacgttc accgggaacc
    57001 ggggggtcaa ctttggtaat cgacagtggg cgataaatgc cgtagctgcc gatagttgcg
    57061 tagactcgcg ctaactgtaa cggtgttacc attagcccgt agccgaaaga gaaggtggcc
    57121 ctctctatgt cagaccaccg ttgtttttga ggatataagc cactgcgttc tccgaccaac
    57181 cccaaattgg tcgcttttcc cagtccaaaa cgtgagtaag tatctactaa cgctgaggac
    57241 ggcatcgcta acgccagctt ggaaacaccg acgttactcg acttctgtaa taccccggtc
    57301 agggttaatt cgctgtagcg tgccacgtct ttgatttcgt ggccgttaat tcgataagga
    57361 atggtattga gtaccgagtt ttcccgcacc acgccacgtt gcaacgcggt catcaccacc
    57421 atcggtttaa ccgttgagcc cggttcaaac acgtcggtga tggtacggtt acgcatcgcc
    57481 tctttcggcg tgccgctcag attgttaggg ttgtatgacg ggctgttagc catcgccagc
    57541 acttcacctg tgttgacatc caccagcacg gcgctaccag attcagcctt gttaaaggcc
    57601 accgcattgt tcagttcgcg ataaaccagc gcctgcaggc gttcatcaat actcagcgcc
    57661 aggttgtgcg ctgcctggct gtcagtagaa gaaatatctt caattacgcg accatagcgg
    57721 tctttacgca caatgcgctc acccggctgc ccggtaagcc atttatcgaa actcttctca
    57781 acgccctcaa tcccttgact atcgacgtta gtaaagccga tgaggtgagc agtcacttcg
    57841 ccggacggat agtaacggcg agactcttca cgcagatgaa tccccggcag tttcagtttt
    57901 ttgatgtagt ccgccatgtc agagtttacc tgacgcgcca gataaataaa gcgccctttc
    57961 gggttggcgt taatgcgggc agaaagctga tccagcggaa tattgagcgc gttagccagc
    58021 gccttccagc ggtcaccgac gctgataccg ccagcgtcat gcacttcttt cgggtcagcc
    58081 caaatcgctt ttaccggcac gctcaccgct aacgggcgac cagaacggtc agtaatcatg
    58141 ccgcgggagg tggaaacttg ctgaacgcga agagaacgca tgtcgccctc tttcaccagc
    58201 atatccgggg agataacttg taaccacgct acgcgtccga gcagaaaagc cagcgccagg
    58261 agaatacagc cgcataacaa cgcaaaacgc caactgataa agttggcatg ttcttcctga
    58321 cgttttggtt tctgcgtttt cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc
    58381 actacgatat tttcttgtga cggatcaaca tgctgcattt gcagcttttc cgtggcgatc
    58441 ctttccaccc ggctatggtc gccgagcgca ttctcttcaa ggatcaggtt gcgccattca
    58501 atgtctaaag catctcgctc cagcaccagt tgttcgcgct gagcggtcag taaacgggta
    58561 tggtgcgccg tggttaccac ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt
    58621 ggcagcttcc caaatcgcaa aagatcgtca ccgataacac caggcaatgc atggcgctcg
    58681 tggcttccca tcgatccttt aactttgctt agagcttctg tcactctgct gatcatgcgt
    58741 tcgtcctctc tgcaatacgc agaactgaac tacgggcacg agggttctca gccacctctt
    58801 cttcgcccgg cattaacttg cctagtgctc gcagctgacg gccacccagt tttttgagct
    58861 gctcttcagt catcggtaac cctgccggaa cttgcggacc gcggctgttt tcacgcataa
    58921 aacgtttcac aatacggtct tccagcgagt ggaagctgat gatcgaaagc cgcccacccg
    58981 gggccagcac gttgagcgag ctttttagcg cctgctctat ctcctccagt tcactgttta
    59041 cccaaatgcg caccgcctgg aaggtacggg tcgcgggatg tttaaactta tctttcaccg
    59101 gcgttgcagc agccacgact tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt
    59161 tacgctcgac aatggcgcgg gcaatgcgtt tggcaaaacg ctcttcacca taggttttca
    59221 atacccaggc gatatcggct tcttctgcgg tttgtagcca ttcagcggct gactgcccac
    59281 gggttgggtc catacgcatg tccagcggac catcgcgcat aaaggaaaag ccgcgttcag
    59341 catcatcaag ttgcggtgaa gagacgccaa gatcgaggag aatgccgtcg atcttgccaa
    59401 taagatcgcg ctcggcaacg tattcgccca gcgcggagaa aggtccgtgg atgatggaga
    59461 agcgcggatc atcaatagtc ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca
    59521 gcaaacgccc ctcttcgcca agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa
    59581 aagtcccatc aatgtagatg ccatcaggac ggatattgag gccattaacg gcttcatcca
    59641 gcagcaccgt agtatgttta tagttttcca tcattttata gagacaagtc ctgcagtcgc
    59701 tccgataagt ctccggtagc caactgctct gcgtcgatat cttccttgac ctgttgatgc
    59761 caggttgttt catcccacag ctcaaacttg ttgaactgtc caaccagcat cacttctttt
    59821 gtcagcccgg catgttgccg cagtactggc gcgattaaca atcgacctgc gccatccatc
    59881 tgacattcgc tggcatgacc taacagtagg cgctgcacac ggcgctcaac cgggttcatg
    59941 ctcgacagac gcgataattt ttgctcgata atttcccatt caggcagggg gtaaagcagc
    60001 aggcacgggt gatgaatgtc aatggtgcaa accatttgac cggcagcgtt ctcaagcagc
    60061 tgttcccgat aacgggtagg cactgataag cgccctttgc tgtcgagatt gactaacgtt
    60121 gctccccgga acatgccagc ctcacccctt attcccactt tgccccacaa tttcccactg
    60181 aaaggagttt acggagcgga ggaaaagctt gtcaagccag tcacaaggca taccgaggta
    60241 aaaaacccga tgtttacggc taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc
    60301 aaattaacgt tatgaatagc tgtaagaaaa aatccctcat cgtcatcact gttttaaaat
    60361 gactcactat tttccacagc aaatataaag tgtcagtttg cgacgcgagc ggcattttag
    60421 gacatatctt ccccggttaa cagtccttgt tacgtctgtg tggcgcttga tgccagcggg
    60481 tttgcgcaaa agcaaagaaa agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa
    60541 aataatgcaa aaacgcgctg tcgatatcac ttaattattg tgaatttcag ctggtgagtt
    60601 aattaacggg cgggtttaaa accatttatc aataatagtc aggaatagtc ttatttactt
    60661 taaggatatt gat
//
LOCUS       DAJTYF010000011        57755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTYF010000011 DAJTYF010000000
VERSION     DAJTYF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.011"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            81..653
                     /gene="yeiP"
                     /locus_tag="OO590_000787"
     CDS             81..653
                     /gene="yeiP"
                     /locus_tag="OO590_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6636261.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            876..2342
                     /locus_tag="OO590_000788"
     CDS             876..2342
                     /locus_tag="OO590_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6636262.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNPLKI"
     gene            2460..3446
                     /gene="yeiR"
                     /locus_tag="OO590_000789"
     CDS             2460..3446
                     /gene="yeiR"
                     /locus_tag="OO590_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6636263.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3485..4198
                     /gene="lpxT"
                     /locus_tag="OO590_000790"
     CDS             3485..4198
                     /gene="lpxT"
                     /locus_tag="OO590_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6636264.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4610..5176
                     /gene="mepS"
                     /locus_tag="OO590_000791"
     CDS             4610..5176
                     /gene="mepS"
                     /locus_tag="OO590_000791"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6636265.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5357..6913
                     /locus_tag="OO590_000792"
     CDS             5357..6913
                     /locus_tag="OO590_000792"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6636266.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            6995..8809
                     /locus_tag="OO590_000793"
     CDS             6995..8809
                     /locus_tag="OO590_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6636267.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            8810..9904
                     /locus_tag="OO590_000794"
     CDS             8810..9904
                     /locus_tag="OO590_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6636268.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            9904..10929
                     /locus_tag="OO590_000795"
     CDS             9904..10929
                     /locus_tag="OO590_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6636269.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            10931..12520
                     /gene="yejF"
                     /locus_tag="OO590_000796"
     CDS             10931..12520
                     /gene="yejF"
                     /locus_tag="OO590_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6636270.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     LSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12524..12868)
                     /locus_tag="OO590_000797"
     CDS             complement(12524..12868)
                     /locus_tag="OO590_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636271.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13201..14391)
                     /gene="bcr"
                     /locus_tag="OO590_000798"
     CDS             complement(13201..14391)
                     /gene="bcr"
                     /locus_tag="OO590_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR6636272.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14419..15114)
                     /gene="rsuA"
                     /locus_tag="OO590_000799"
     CDS             complement(14419..15114)
                     /gene="rsuA"
                     /locus_tag="OO590_000799"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6636273.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15264..17024
                     /locus_tag="OO590_000800"
     CDS             15264..17024
                     /locus_tag="OO590_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6636274.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17149..17433
                     /gene="rplY"
                     /locus_tag="OO590_000801"
     CDS             17149..17433
                     /gene="rplY"
                     /locus_tag="OO590_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6636275.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17572..18579)
                     /gene="yejK"
                     /locus_tag="OO590_000802"
     CDS             complement(17572..18579)
                     /gene="yejK"
                     /locus_tag="OO590_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6636276.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            18761..18988
                     /locus_tag="OO590_000803"
     CDS             18761..18988
                     /locus_tag="OO590_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6636277.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19008..20768
                     /gene="pbgA"
                     /locus_tag="OO590_000804"
     CDS             19008..20768
                     /gene="pbgA"
                     /locus_tag="OO590_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6636278.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            20843..20919
                     /locus_tag="OO590_000805"
     tRNA            20843..20919
                     /locus_tag="OO590_000805"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20877..20879,aa:Pro,seq:ggg)
     gene            complement(21022..23613)
                     /gene="yejO"
                     /locus_tag="OO590_000806"
     CDS             complement(21022..23613)
                     /gene="yejO"
                     /locus_tag="OO590_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6636279.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            23933..24580
                     /gene="narP"
                     /locus_tag="OO590_000807"
     CDS             23933..24580
                     /gene="narP"
                     /locus_tag="OO590_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6636280.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(24791..25843)
                     /gene="ccmH"
                     /locus_tag="OO590_000808"
     CDS             complement(24791..25843)
                     /gene="ccmH"
                     /locus_tag="OO590_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6636281.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(25840..26397)
                     /gene="dsbE"
                     /locus_tag="OO590_000809"
     CDS             complement(25840..26397)
                     /gene="dsbE"
                     /locus_tag="OO590_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6636282.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26394..28337)
                     /gene="ccmF"
                     /locus_tag="OO590_000810"
     CDS             complement(26394..28337)
                     /gene="ccmF"
                     /locus_tag="OO590_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6636283.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28334..28813)
                     /gene="ccmE"
                     /locus_tag="OO590_000811"
     CDS             complement(28334..28813)
                     /gene="ccmE"
                     /locus_tag="OO590_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6636284.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(28810..29019)
                     /gene="ccmD"
                     /locus_tag="OO590_000812"
     CDS             complement(28810..29019)
                     /gene="ccmD"
                     /locus_tag="OO590_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6636285.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29016..29753)
                     /gene="ccmC"
                     /locus_tag="OO590_000813"
     CDS             complement(29016..29753)
                     /gene="ccmC"
                     /locus_tag="OO590_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6636286.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(29795..30457)
                     /gene="ccmB"
                     /locus_tag="OO590_000814"
     CDS             complement(29795..30457)
                     /gene="ccmB"
                     /locus_tag="OO590_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6636287.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30454..31071)
                     /gene="ccmA"
                     /locus_tag="OO590_000815"
     CDS             complement(30454..31071)
                     /gene="ccmA"
                     /locus_tag="OO590_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6636288.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31090..31692)
                     /gene="napC"
                     /locus_tag="OO590_000816"
     CDS             complement(31090..31692)
                     /gene="napC"
                     /locus_tag="OO590_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6636289.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(31702..32151)
                     /gene="napB"
                     /locus_tag="OO590_000817"
     CDS             complement(31702..32151)
                     /gene="napB"
                     /locus_tag="OO590_000817"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6636290.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32148..33011)
                     /gene="napH"
                     /locus_tag="OO590_000818"
     CDS             complement(32148..33011)
                     /gene="napH"
                     /locus_tag="OO590_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6636291.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(32998..33693)
                     /gene="napG"
                     /locus_tag="OO590_000819"
     CDS             complement(32998..33693)
                     /gene="napG"
                     /locus_tag="OO590_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6636292.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(33700..36186)
                     /gene="napA"
                     /locus_tag="OO590_000820"
     CDS             complement(33700..36186)
                     /gene="napA"
                     /locus_tag="OO590_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6636293.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTAMKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36183..36446)
                     /gene="napD"
                     /locus_tag="OO590_000821"
     CDS             complement(36183..36446)
                     /gene="napD"
                     /locus_tag="OO590_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6636294.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36436..36930)
                     /gene="napF"
                     /locus_tag="OO590_000822"
     CDS             complement(36436..36930)
                     /gene="napF"
                     /locus_tag="OO590_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6636295.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRVWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37338..37826
                     /gene="eco"
                     /locus_tag="OO590_000823"
     CDS             37338..37826
                     /gene="eco"
                     /locus_tag="OO590_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6636296.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(37976..39007)
                     /locus_tag="OO590_000824"
     CDS             complement(37976..39007)
                     /locus_tag="OO590_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR6636297.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQSTLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39241..39600)
                     /locus_tag="OO590_000825"
                     /pseudo
     CDS             complement(39241..39600)
                     /locus_tag="OO590_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(39818..41461)
                     /gene="yojI"
                     /locus_tag="OO590_000826"
     CDS             complement(39818..41461)
                     /gene="yojI"
                     /locus_tag="OO590_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6636298.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41537..42131)
                     /gene="alkB"
                     /locus_tag="OO590_000827"
                     /pseudo
     CDS             complement(41537..42131)
                     /gene="alkB"
                     /locus_tag="OO590_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            complement(42131..43195)
                     /gene="ada"
                     /locus_tag="OO590_000828"
     CDS             complement(42131..43195)
                     /gene="ada"
                     /locus_tag="OO590_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6636299.1"
                     /translation="MKNATCLTDDQRWQSVLARDPDADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43269..44324)
                     /gene="apbE"
                     /locus_tag="OO590_000829"
     CDS             complement(43269..44324)
                     /gene="apbE"
                     /locus_tag="OO590_000829"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6636300.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44436..45557)
                     /gene="ompC"
                     /locus_tag="OO590_000830"
     CDS             complement(44436..45557)
                     /gene="ompC"
                     /locus_tag="OO590_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6636301.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46295..48967
                     /gene="rcsD"
                     /locus_tag="OO590_000831"
     CDS             46295..48967
                     /gene="rcsD"
                     /locus_tag="OO590_000831"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6636302.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQILIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            48984..49634
                     /gene="rcsB"
                     /locus_tag="OO590_000832"
     CDS             48984..49634
                     /gene="rcsB"
                     /locus_tag="OO590_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6636303.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(49834..52683)
                     /gene="rcsC"
                     /locus_tag="OO590_000833"
     CDS             complement(49834..52683)
                     /gene="rcsC"
                     /locus_tag="OO590_000833"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6636304.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKKTLTVYAERVRKSRDS"
     gene            complement(52958..53734)
                     /locus_tag="OO590_000834"
     CDS             complement(52958..53734)
                     /locus_tag="OO590_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6636305.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDMDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(53739..55388)
                     /locus_tag="OO590_000835"
     CDS             complement(53739..55388)
                     /locus_tag="OO590_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6636306.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55389..56111)
                     /locus_tag="OO590_000836"
     CDS             complement(55389..56111)
                     /locus_tag="OO590_000836"
                     /inference="COORDINATES: protein motif:HMM:NF036531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636307.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEE
                     EMSFTLQPVTSNEIDSDALYLDEITLTCEQDAVLRYGQVEVPLPPGADVERTTWGISV
                     NKPNAAEQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYV
                     RSYAPAQQSVAAGSEWTGMQVK"
     gene            complement(56108..57667)
                     /locus_tag="OO590_000837"
     CDS             complement(56108..57667)
                     /locus_tag="OO590_000837"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636308.1"
                     /translation="MTTSELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFY
                     PLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMPERPRREEVDTAAWMPS
                     LTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVY
                     RVGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLS
                     AELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETP
                     QEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRL
                     MQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQA
                     DNMPLLHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILD
                     TPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGG
                     DATQTAAILMV"
BASE COUNT        14107 a        15596 c        14190 g        13862 t
ORIGIN      
        1 cgaataccac ttgcccgaaa ggcccatcgc gagtactttg tcgcgatatt tttgacattt
       61 tcgactacag gaatttttcg atgccaagag cgaacgaaat taaaaaaggt atggtactga
      121 attacaacgg caaactgctg ctggctaagg atattgatat tcagtcgccc actgcccgcg
      181 gtgccgctac gctgtacaaa atgcgttttt ctgatgtccg taccgggctg aaagtagaag
      241 agcgtttcaa aggtgatgat atcgttgaca ccgtgacgct gacccgccgt tacgttgact
      301 tctcctatgt cgatggcaac gaatatgtct ttatggataa agaagactat accccgtata
      361 ccttcaccaa agatcagatt gaagaagagt tgctgtttat gccagagggc ggcatgccgg
      421 acatgcaggt gctgacctgg gacggtcaac tgctggcgct tgagctgccg cagaccgttg
      481 atctggaaat cgttgaaacc gcaccaggca tcaaaggggc atctgccagc gcccgtaaca
      541 aaccggcgac attgagcact ggtctggtga ttcaggtacc agaatactta agcccgggcg
      601 aaaaaattcg tatccatatc gaagaacgcc gttatatggg gcgtgctgac taacttcagc
      661 cgcatgcaga aaagggatag ctcaggctgt ccctttttta atttattata ccaatcttct
      721 attttgcgct tcctgaacac cttacgccct caattttcac tcgttgattg atcgccctca
      781 cacttcatcg cattaacaat ccagaccaat ttcaattgct gtcatataac tttacactgt
      841 cattgttaat ttatcgttac taagacgtga ctcctatgaa gacaattgcc tccgttacgc
      901 tcccgcatca tgtacacgct ccacgctacg atcgccagca gttgcaatca cgtatcgttc
      961 attttggctt tggcgccttt caccgcgctc atcaggcgtt actgaccgat cgtgtgctga
     1021 atgcccaggg cggcgactgg gggatctgtg aaatcagctt gttcagcggt gatcaactga
     1081 tgagccagct ccgcgcacag aaccatttat ataccgtgct ggagaaaggt gcggacggca
     1141 atcaggtgat aattgtcggt gccgttcacg aatgccttaa tgcaaaactg gattccttag
     1201 cggcaattat tgagaaattt tgcgagccac aggtggcaat tgtttccctg acgattaccg
     1261 aaaaaggcta ttgtattgac ccggccaccg gtgcactcga caccagtaat ccgcggatta
     1321 ttcacgatct acaaacccct gaagaacctc actccgcacc gggtattctc gtcgaagcac
     1381 tgaaacgccg ccgtgagcgc ggccttacac cgtttaccgt gctctcctgc gacaatattc
     1441 ccgacaatgg ccatgtggtg aaaaacgcgg tgctgagaat ggcagaaaaa cgttcgccag
     1501 aactcgccgg gtggataaaa gaacacgtca gttttccggg aaccatggtc gaccgcattg
     1561 ttccggctgc aaccgacgaa tcactggcag aaatcagcca gcatctgggg gtaaatgatc
     1621 cctgcgcgat tagctgcgaa ccgtttatcc agtgggtggt tgaagataac ttcatcgctg
     1681 ggcgtcctgc ctgggaagtc gcaggtgtac aaatggtgaa tgatgtcctg ccatgggaag
     1741 agatgaaact gcgtatgctt aatggcagcc actcttttct cgcttatctg ggttacctct
     1801 caggattcgc ctatatcagt gattgcatgc aggatcgcgt atttcgctat gctgccagaa
     1861 cattaatgct ggatgagcaa gcgccgacac tgcaaattaa agatgtcgat ttaacacaat
     1921 atgcggataa gttaattgca cgttttgcta atccgtcgct gaaacataag acctggcaaa
     1981 tcgcgatgga tggcagccag aaattaccgc aacgtatgct ggcaggtatt cgcatacatc
     2041 tggggcgtga aacggactgg tcgttgctgg cattaggcgt tgctggctgg atgcgttacg
     2101 tcagcggcgt tgatgatgcc ggaaatgcca ttgatgttcg cgatccgctt agcgataaaa
     2161 ttcgcgaact tgttgcgggc agcagcagtg aacaacgcgt aaccgccctg ctttccctgc
     2221 gtgaagtttt cggtgatgat ctgccagata acccgcattt tgtgcaggcc atcgaacaag
     2281 cctggcaaca aatcgtacaa ttcggcgcac atcaggcgct attaaacccc ctcaaaattt
     2341 aacgatttct gcggttaaag cggatgaagc tcaccttcgt ccgctctccc cttctctttt
     2401 ctgccttttt tagccaggat taacgctcag ttaacttacc agagtattgc ggagccgttg
     2461 tgaccaggac caacctcatc accggttttc tcggcagcgg gaaaaccacg tcgattcttc
     2521 atctgttagc ccataaagat cccaacgaaa aatgggcggt actggttaat gaatttgggg
     2581 aagtcggaat tgatggtgct ttgctcgccg atagcggcgc attgctgaaa gagatccccg
     2641 gcggctgcat gtgctgcgtt aatggtttac ccatgcaggt agggttgaat accttactgc
     2701 gtcagggaaa accagaccgc ttgttgatag agccgaccgg gctgggccat ccgaaacagc
     2761 tcctcgatct gttaaccgca ccagtctatg aaccgtggat agatctgcgc gccaccttgt
     2821 gcattctcga tccacgcctg ctgctggacg aaaaaagcgc cagcaatgaa aacttccgtg
     2881 accagctggc tgccgcagac atcattgtcg ccaataaatc cgaccgtgcg acgcccgaaa
     2941 gtgagcaagc gctacagcgt tggtggcagc aaaatggtgg cgatcgacaa ttaattcaca
     3001 gtgagcatgg gaaagttgac ggtcatcttc tggatttgcc gcgtcgcaat ttagccgagt
     3061 tgcccgccag cgccgcgcat tctcatcagc atgtcgtgaa aaaagggtta gcagcgttaa
     3121 gcctgccaga gcatcaacgc tggcgtcgca gtctgaacag cgggcaagga tatcaggcct
     3181 gcggctggat attcgacgct gatacggtat tcgacaccat tggcattctg gaatgggcgc
     3241 gacttgcacc ggtggaacgc gtcaaaggcg tgctgcgtat tcccgaaggg ctggtacgaa
     3301 tcaaccgtca gggcgatgac ctgcacattg aaacgcaaaa cgttgcgcca ccggacagcc
     3361 gtattgagct gatttccagc agcgaagctg actggaatgc cttgcagagc gcgctgttga
     3421 agcttcgttt agcgactacc gcgtaaggtt gcctgcgttt ttcagtaaga taattagaga
     3481 aaatatgatt aaaaatttgc cgcaaatagt gttgttgaat attgtcggcc ccgcgctgtt
     3541 tctttcctgg tatattcccg ttaatcatgg attctggttg ccgattgatg cggatatttt
     3601 ttatttcttt aatcagaaac tggtcgaaag taaggccttt ttatggctgg ttgcattgac
     3661 taacaatcgc gccttcgacg gttgttcact gctggcgatg ggtatgttga tgctgagttt
     3721 ctggctgaaa gaaaacgccc ctggcagacg acgtatcgtg attatgggtc tggtcatgct
     3781 attaactgca gtggtattaa accagctggg tcaggcatta attcctgtaa aacgggccag
     3841 cccaacattg acttttaccg atattaaccg cgtcagcgaa ctgctctctg ttcccacgaa
     3901 agatgcctca cgagatagct ttcccggcga tcacggcatg atgctgctta ttttttcggc
     3961 attcatgtgg cgttatttcg gcaaagttgc aggccttatc gcccttatta tttttgtggt
     4021 ttttgcattt cccagagtaa tgattggcgc acactggttt actgacatca ttgtcggttc
     4081 gatgaccgtg atattgatcg gtttgccctg ggtgttgctg acgccattaa gtgatcgatt
     4141 aatcaccttt tttgacaaat cactaccagg aaaaaacaaa catttccaaa acaaataact
     4201 cacagtaatt aacatcatca gggttatttt tatagtgagg ataatcctga tgatgcgcac
     4261 cgtgctttca tctatcgaac gcaaaaatca ttctctaagt aaatgaatgg attgcatgcg
     4321 tttcactcaa ttgtacttta attgaccaac cccgcttatt aactttctgt atcacttttt
     4381 cttataaaaa atcatgtaaa accgctcgcc aagaccgcac caatcgggta atctcgaact
     4441 cgttttgcct cggcggtaga ttatcctcac agcatataat tttgtgcgtt agtccacaga
     4501 tttggcctta aggaattgtt tcaacatgcc caggtaatta gtctcgtgtc gcctggcatt
     4561 tttttataac gatatttgtc gttaaggact tcaagggaaa acaaacaaca tggtcaaatc
     4621 tcaaccgatt ttgagatata tcttgcgcgg gattcccgcg attgcagtag cggttctgct
     4681 ttctgcatgt agtgcaaata acaccgcaaa gaatatgcat cctgagacac gtgcaatggg
     4741 tagtgaaaca tcatcactgc aagcttctca ggatgaattt gaaaacctgg ttcgtaatgt
     4801 cgacgtaaaa tcgcgaatta tggatcagta tgctgactgg aaaggcgtac gttatcgtct
     4861 gggcggcagc actaaaaaag gtatcgattg ttctggtttc gtacagcgta cattccgtga
     4921 gcaatttggc ttagaacttc cgcgttcgac ttacgaacag caggaaatgg gtaaatctgt
     4981 ttcccgcagt aatttgcgta cgggtgattt agttctgttc cgtgccggtt caacgggacg
     5041 ccatgtcggt atttatatcg gcaacaatca gtttgtccat gcttccacca gcagtggtgt
     5101 tattatttcc agcatgaatg aaccgtactg gaagaagcgt tacaacgaag cacgccgggt
     5161 tctcagccgc agctaataaa ccgtttggat gcaatccctt ggctatcctg acgagttaac
     5221 tgaaagcact gcttaggcag tgcttttttg ttttcattca tcagagaaaa tgatgtttcc
     5281 gcgtcttgat ccaggctata gtccggtcat tgttatcttt taaatgttgt cgtaatttca
     5341 ggaaattaac ggaatcatgt tcatacgcgc tcccaattct ggacgtaagc tcctgcttac
     5401 ctgcattgta gcaggcgtga tgattgcgat actggttagc tgccttcagt ttttagtggc
     5461 caggcataag cacgaagtca aatacgacac actgattatc gacgtacaaa agtatctcga
     5521 tacctatttt gccgacctga aatccactac tgaccggctt cagccgctga ccttagatac
     5581 ctgccagcag gctaaccccg aactgaccgc tcgcgcggcg tttagcatga atgtccgtac
     5641 gtttgtgctg gtgaaagata aaaaaacatt ctgttcatct gcgactggtg agatggacat
     5701 tccactcaat gaattgattc cggcgctcga cattaataaa aacgtcgata tggcgatctt
     5761 acccggcacg ccgatggtgc cgaacaaacc cgcaatcgtc atctggtatc gcaacccttt
     5821 gctgaaaaat agcggtgtgt ttgccgctct gaatctcaac ctgacgcctt ctctctttta
     5881 tagttcacgg caggaagatt acgatggcgt cgccctcatt attggcaata ctgcgctatc
     5941 taccttttct tcacgtttga tgaacgttaa tgaattaacc gacatgccgg tccgtgaaac
     6001 taaaattgcg ggcattcctc tgaccgttcg gctttatgcg gatgactgga catggaacga
     6061 tgtgtggtac gcatttttac tgggcggcat gagtggaact gtcgttggcc tgctttgcta
     6121 ttacctgatg agcgtacgta tgcgccccgg cagagaaatc atgaccgcca tcaagcgcga
     6181 acaattttac gtggcgtatc aaccggtggt ggatacacaa gctttgcggg taacgggcct
     6241 ggaagtactg ctacgctggc ggcatcctgt agcgggagaa attcccccgg atgccttcat
     6301 taactttgcc gaatcgcaaa agatgattgt gccgctgact cagcacctgt ttgagttaat
     6361 tgcccgcgat gccgcagaat tagaaaaagt actgccggta ggcgtcaaat ttggcattaa
     6421 cattgcgccg gaccatctgc acagcgaaag ctttaaagca gatatccaga aactgctcac
     6481 ttccctgccc gcacaccatt tccagattgt gctggaaatt accgagcgcg atatgctgaa
     6541 agagcaagaa gccacacaac tcttcgcctg gctgcacttg gtcggcgtag aaattgctat
     6601 tgatgacttc ggcaccgggc acagcgcgct tatctatctt gagcgtttta cgctcgatta
     6661 tctgaaaatt gaccgtggat ttatcaacgc catcggtacg gaaacgatca cttcacccgt
     6721 acttgacgcg gtgctgacgc tggcgaaacg tctcaatatg ctgacagtta ctgaaggggt
     6781 cgaaacgccg gaacaagcgc gatggctaag cgaacgcggc gttaatttca tgcaaggcta
     6841 ctggattagt cgcccgttac cgctggacga ttttgttcgc tggctgaaga aaccgtatac
     6901 gccgcagtgg taaggtgtgc ttacgtccct tattattcat agtgaaagca tgccggattg
     6961 cggctaatga tgagtaaaag gaaatccgtt gcagatgatt gtgcgcatac tgctgctgtt
     7021 tatcgctctg ttcaccttcg gtgcgcaggc gcaggctatc aaggaaagct atacctttgc
     7081 cgtgctgggc gaaccccggt acgcgtttaa tttcaaccat tttgattatg tgaaccccgc
     7141 cgcgccaaaa ggtgggcaga taacgttgtc agccctcggt accttcgata atttcaaccg
     7201 ctatgcactg cgcggcaacc cgggcgcacg caccgagcag ctgtacgaca cgttatttac
     7261 gacttccgat gacgaaccag gcagttatta cccgctgatt gctgaaagtg cacgctatgc
     7321 tgacgattat tcctgggtgg aggtcgctat taatccgcgc gcccgttttc atgatggttc
     7381 tcccattact gcccgcgatg tagagtttac ttttcaaaaa tttctgaccg aaggcgtgcc
     7441 gcaatttcgt ctggtctaca aaggcaccac cgtcaaagcc attgcgccgt taaccgtgcg
     7501 cattgagtta gctaaacccg gcaaagaaga tatgttgagt ctgttttcgc tgccggtatt
     7561 tccagaaaag tactggaagg atcacaaact tagcgacccg ctcgccacgc ctccgcttgc
     7621 cagtggtccg taccacgtta cgtcctggaa aatggggcaa aatattgtct attcccgcgt
     7681 gaaagattac tgggcagcaa acttaccggt aaaccgtgga cgctggcatt tcgacaccat
     7741 tcgctacgat tattacctcg atgataatgt cgcctttgaa gcgtttaaag caggtgcctt
     7801 tgatttgcgt atggaaaacg acgctaaaaa ctgggccacg cgttataccg gtaaaaattt
     7861 cgataaaaaa tacatcatca aagatgagca aaagaacgaa tcagcccagg atacgcgctg
     7921 gctggcgttt aatatccaac gtgcggtatt cagcgatcgc cgggtgcggg aagcaatcac
     7981 tctcgccttt gactttgaat ggatgaacaa ggcgttgttt tacaacgcct ggagtcgcac
     8041 gaacagttat tttcaaaata ccgaatacgc ggccagaaat taccccgacg ccgcggagct
     8101 ggtgcttctg gcaccaatga aaaaagatct accgccagaa gtcttcacac aaatctacca
     8161 gccgccggta tctaaaggcg atggctacga tcgtgacaac ctgttaaaag ccgacaaact
     8221 tctcaacgaa gcgggctggg tgctgaaggg tcagcaacgc gttaatgcca caacgggtca
     8281 gccgctcagc tttgaattat tgcttcccgc aagcagcaat agtcagtggg tattgccgtt
     8341 ccagcacagc ctgcaacggc tgggtatcaa tatggacatt cgcaaggtgg ataactcgca
     8401 aatcactaac cgcatgcgca atcgcgacta tgacatgatg ccgcgcctat ggcgggcgat
     8461 gccgtggccc agttccgatt tacagatttc ctggtcatcg gaatatatca attccactta
     8521 taatgccccc ggcgtgcaaa gcccggttat cgactcgctg atcaaccaaa ttattgccgc
     8581 gcagggaaat aaagaaaaat tactgccgtt ggggcgagca ctggatcgcg tattaacgtg
     8641 gaattattac atgctgccaa tgtggtacat ggcggaagac cgtctcgcct ggtgggataa
     8701 attctcccaa cccgctgtac gccctgttta cagcctaggt atcgatacct ggtggtatga
     8761 cgttaataaa gcggccaaac tgccgtcagc cagacaacag ggagagtaga tgggcgctta
     8821 cctgattcgc cgtctgttgc tggtgatccc aacgttatgg gctattatca ctatcaactt
     8881 tttcatcgtg caaattgcgc ctggcggtcc ggtcgatcag gccatcgccg ccattgagtt
     8941 tggtaatgcc ggagtattac ccggcgcagg cggtgaaggt gttcgtgcca gccatgcgca
     9001 aacgggtgtc ggcaatatca gcgacagtaa ttaccgtggc ggacgcggat tagatccaga
     9061 agtgatcgct gagatcactc atcgctacgg tttcgataag ccgatccacg aacgttactt
     9121 caaaatgctc tgggactaca tccgctttga ttttggtgat agcctgtttc gcagcgcgtc
     9181 ggtgctgacg ctgattaaag acagtctgcc ggtttccatc accctcggat tgtggagcac
     9241 gctgattatc tatctggtgt cgattccgtt aggcattcgc aaagctgttt ataatgggag
     9301 tcgctttgac gtctggagta gcgcatttat catcatcggc tacgccattc cggccttttt
     9361 gtttgccatc ctgctgattg tcttcttcgc gggcggcagc tatttcgacc tgttccctct
     9421 acgcggcctg gtttccgcta actttgattc gctgccgtgg tatcagaaaa tcaccgatta
     9481 tctgtggcat atcacgctac cggtgctggc gacagtgatt ggtggctttg cggcgctgac
     9541 catgctgacc aaaaactcat tccttgatga agtgcgcaag caatacgtgg tgaccgcgcg
     9601 tgcgaaaggg gtaagtgaga aaaacattct ctggaaacat atgttccgca acgccatgct
     9661 gctggtgatt gccggttttc cggcgacgtt tatcagcatg ttttttaccg gctcgctgct
     9721 gattgaggtg atgttttcac tcaatggtct tggcttactg ggctacgaag cgaccgtctc
     9781 gcgcgattat cctgtaatgt ttggtacctt gtatattttc accctgattg gcctgctgct
     9841 gaatattgtc agtgatatca gctatacgct ggttgatccg cgtatagatt ttgagggacg
     9901 ttaatgtcgc gactcagccc cgtcaatcag gcccgttggg cgcgttttcg tcataaccgt
     9961 cgcggctact ggtcgttatg gattttcctc gttttgtttg gtttgagttt gtgttctgaa
    10021 cttatcgcta acgataaacc gttgctggtg cgttatgacg gcagttggta tttcccgttg
    10081 ttgaaaaact acagcgaaag cgattttggc ggcccgctgg caagtcaggc tgattatcag
    10141 gacccgtggc tgaaacaacg gctggaaaat aacggctggg tactgtgggc accgattcgc
    10201 tttggtgcta ccagtatcaa ctttgctacc gataagccct tcccttctcc accctcccgg
    10261 caaaactggc tgggaacgga tgcaaacggc ggcgatgtgc tggcacgcat tctctatggc
    10321 acgcggatct cggttctgtt tggcctgatg ctgactctct gttccagcgt gatgggcgtg
    10381 ctggcggggg cgctacaagg ctattacggc ggtaaagtcg atctctgggg gcaacgcttt
    10441 attgaagtat ggtcggggat gccgacgctg tttttgatta ttttactttc cagcgtcgta
    10501 cagcctaact tctggtggct gctggcaatt actgtcttgt ttggctggat gagtctggtc
    10561 ggcgtggtgc gggcggagtt tttacgtact cgtaatttcg actacattcg tgcggcacag
    10621 gcgcttggcg tcagcgatcg cagtatcatc ctgcgtcata tgttgcctaa tgccatggtc
    10681 gcgaccctca cctttttacc gtttatttta tgtagttcga taaccaccct gacctcgctc
    10741 gatttcctcg gcttcggtct accgctcggt tcaccgtcac tcggtgaact gctgttacaa
    10801 gggaaaaata accttcaggc cccgtggctt gggatcaccg ccttcttgtc ggtggcgata
    10861 ttattgtctt tgctgatctt tattggtgaa gccgtccgcg acgcatttga tcctaataag
    10921 gcggtgtagc atgacgcaaa ctctgttagc gattgaaaat ttgtcggtgg gttttcgcca
    10981 tcagcaaacc gtacgtacag tagtcaatga tgtttcacta cagattgagg ctggcgaaac
    11041 gctggcgctg gtgggtgagt caggttcagg caaaagcgtt accgcgctgt caattttacg
    11101 cctgctccct tccccgccgg ttgaatatct ctccggcgat attcgttttc atggcgaatc
    11161 gctgcttcat gccagcgatc aaacgttgcg cggtgtacgc ggtaataaga tcgccatgat
    11221 ttttcaggaa ccgatgctgt cgttaaatcc attgcatacc ctggaaaaac agctttatga
    11281 agtgctttca ctccaccgcg ggatgcgtcg ggaagcggct cgtggcgaaa ttcttaactg
    11341 ccttgatcgc gtcggtatcc gccaggcggc aaaacggctg acagattatc cgcatcagct
    11401 ctccggcggc gaacgccagc gggtgatgat tgcgatggcg ctgttaacgc gaccggaatt
    11461 attaattgcc gatgaaccga ccaccgcact ggacgtctct gtccaggcgc agattttaca
    11521 gctgttgcgc gaactgcaag gcgagctgaa tatgggcatg ctgtttatta ctcataacct
    11581 cagcattgtc agaaaactgg cccaccgcgt ggcggtaatg caaaacggtc gctgtgtcga
    11641 gcaaaataac gccgctacgc tatttgcctc acccactcat ccttacacac aaaagctact
    11701 caacagtgaa ccgtcaggcg atccggtacc gttgccagaa cctgcctcaa cgttgctgga
    11761 tgttgaacag cttcaggttg ccttccccat tcgcaaaggg attttgaagc gcattgtgga
    11821 tcataatgtg gtggtgaaaa acatcagttt tacgctacgg gcgggtgaaa cactgggttt
    11881 agtgggcgag tctggttccg ggaaaagcac gacgggactg gcgctgctgc gactgattac
    11941 ctctcagggc agcatcgtct ttgacggtca gccactgcaa gatttaaatc gccgccagct
    12001 gttacctact cgtcatcgta ttcaggtggt atttcaggat ccaaactcct cgctcaaccc
    12061 acgactcaac gttttgcaga ttattgagga aggcttacgg gttcaccagc cgacgctttc
    12121 tgccgcacaa cgcgaacaac aagtgatagc cgtgatgcat gaagtgggat tagatcctga
    12181 aacacgccac cgttatccgg cggagttctc tggtggtcag cgacaacgta ttgcgattgc
    12241 cagggcatta attcttaagc cctcgctgat catacttgat gaaccgacat catcactcga
    12301 caaaacggta caggcgcaaa tattgacgct tttgaaatca ttgcaacaaa agcatcaact
    12361 ggcctatttg tttatcagcc acgatttgca cgttgtccgc gcgttatgtc atcaggttat
    12421 cgtactgcga caaggggaag tagtggaaca aggaccgtgc gcgcgcgtgt ttgccacacc
    12481 gcagcaggag tatacgcgtc agttactggc gttgagctga cgcttaaaaa ggattgtagt
    12541 ttgaaaaggg ttcggcaatt gccacaccaa aatttttcaa tcggcatgtt gcagcaaact
    12601 cgtcctggcg atttacaaac aggcacggct caccttcaca ctccaacacc gaacatggaa
    12661 cttcgatgtc gcttaacgcc tggcttagtt tatacatcac cgaccatgca ttatcaccat
    12721 ccgggctaag caatttaagc gccaccaggc tgttgtcacc gcacggtcca ttttgcggaa
    12781 tcggttcaac cttcgaacct gcgaaaccag cagaccagcg atagttctgc ggcagtcgat
    12841 ggacgattga gagttgtaat gaagtcactt attttccccg gaagtacatt cgcttcacaa
    12901 tttgtttaca tcaattttaa cacatcatca acaatccgtc tttatacaga tcgtaaagat
    12961 tgatgcctcg cctgcgtggc ctctatggct ctgatttaag taatatctgc gggcgtggtc
    13021 acgtttgcgg ggctatatgt acccgtttct gcgatctaac tcaacctttt taactacaat
    13081 gatgtgactt tttacataaa ttgattttac ataaaataaa catatatcgg ggaaatgata
    13141 gatttgtggt tgacatgcat caacaaagga agccttttac cttcctcgtt gtgcaataga
    13201 tcaccgtttt ttcggccgac tggcgtacag acagaagaga atggagctgg ttgcgcagaa
    13261 tgcaattgac caaatcatcg gccatgcaga gttaaaggtc gcaagagaaa gcaatgcgcc
    13321 aacaattgcc cctatgccaa aacggaaggt tcctgccagc gaagatgccg ttcccgccat
    13381 atggggaaac tcatcaagaa tgaccgccat cgcattggat gacaccatcg acacgcagcc
    13441 cacaaacacc gcaacgccaa ccaccagcga ccagaatccc agccccagca gcgcactgat
    13501 gaccatccac gctgccataa taaattgtat ccacaacccc gagcggaaca tatttaacgc
    13561 gccaatgcgg cggacgaagc ggctgttaaa gatggtcatc acgaacagaa aaacaatgtt
    13621 tagcgcaaag taataaccaa agttttccgg cgcgacgtgg ttaatttcaa tataaacaaa
    13681 cggtccggcg cttaagaatg agaacatccc ggcaaagctg aaaccactgg caagcatgta
    13741 gctcaggaca cgtttatggc ggaacagcgc cgcaaagtta ccaatagtgg tacgaatgtg
    13801 aaatggctga cgacgctccg gtggtaaggt ttctttaatc aggaagaaaa tcattgccga
    13861 agccagaatc gccgctaatg ccaggatcca gaagatgtaa tgccagctca gccacaccag
    13921 cacccagccg ccaactatcg gtgccatcag cagtgcaatg gtcgtcacca gcatgacaaa
    13981 cgacatcatc cgcgagaact cttctttcgg gtaaatatca cgcatcaaag cgttgataac
    14041 cacgctggcc gcagccgcag ccagcccgtg gaagaaacgc atcacaatca gctgatcgat
    14101 ggtttgtgcc aacgcacacg ccaccgcggc ggcggcaaac accagcgtcc cgccaagaac
    14161 caccggctta cgcccgaagc tgtctgccat cggcccgtag attaactgcc ccaacgcaaa
    14221 gcccagaata taagtactga gggtcatctg cgtactgccc gccgatacgc caaactgcgc
    14281 tgaaattacc ggtagcgcgg gcagatacat atcaatcgac agcggcatca acatggccag
    14341 caggccaagg ataaaaacaa tagcaaacga cgaatgctgt cgggtggtca caacgggctc
    14401 ctgaaagtca ttgaaaagtt agacgacgct ggcaatttct tcttcagtta acggacgata
    14461 ttcaccgggg gctaaatcag catccagcgt aataccgcca atacgttcac gatgcagctc
    14521 aaccacgtgg ttacccacgg cggcgaacat gcgtttcacc tgatgataac gcccttcgct
    14581 gatggtcaga cgaacctgcg ttggggtaat cacttcaagc accgccggct tagtgagatc
    14641 tttttcgtta tgcagctgca cgcctttagc aaattgctct gccgtatcgt cagctacagg
    14701 tgattccagt gtcaccagat aggtcttctc gcaatgatgg cgcggagaag taatgcggtg
    14761 cgaccactga ccatcatcag tcatcagcac cagaccggtg gtatcgatat ccagacgtcc
    14821 tgcggcgtgc agtttccacg ctaccggttc atccagaaaa tagagcaccg tcgggtgatc
    14881 cgggtcgtcc gtggagcaaa catagccctg aggcttattg agcatgaagt aacgtggacc
    14941 gtgttgctgc gccagcgggt tgccatcgta agcgacatca tgttcaggga gcagtttgaa
    15001 cgctgcatta cgaacgattt cgccatcgac ggtgacacga ttgccgcgga tttcacgccc
    15061 ggcaatagca cggctaacgc cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt
    15121 gattttgcct gtaaaaaacg gaggtcgggt aatgacccga aaatctgaac tgttgtctgc
    15181 ccagtatagc ggtctaataa cttccctcaa gggaaaaaga accgtggcat actattagca
    15241 gaataatcta cctacgcgaa accatgattt ttacactccg cccatatcag caagaagccg
    15301 tggatgccac gctcaaccat tttcgtcgtc ataaaacccc tgccgttatc gtgctgccca
    15361 ccggcgcagg taaaagcctg gtgatagcgg aactggcacg gctggcgcgt ggtcgcgtac
    15421 tggtgctggc acacgttaaa gaactggtgg cgcaaaacca tgcaaagtat caggcgctgg
    15481 ggctggaagc cgatattttt gccgccgggc taaagcgcaa agagagccac ggtaaagtgg
    15541 tatttggcag cgttcagtcg gtcgcccgta atcttgatgc ctttcagggt gaattttcgc
    15601 tgttgattgt cgatgaatgc caccgtattg gtgacgatga agagagccag tatcagcaaa
    15661 tcctcactca cctgactaaa gtgaatcccc acttacgcct gctggggctg actgccacgc
    15721 cttttcgatt gggcaaaggc tggatctacc agttccatta tcacggcatg gtacgcggcg
    15781 atgagaaagc ccttttccgt gactgcattt atgagctgcc gctgcgttat atgattaaac
    15841 acggctatct gacgccgcca gaacgactgg atatgccagt agtgcaatac gatttcagcc
    15901 gcttgcaggc acagagtaac gggctgttca gcgaagctga tctcaaccgt gagctgaaaa
    15961 aacagcaacg cattacgcca catatcatca gccagatcat ggagttcgcc gaaaagcgca
    16021 aaggggtaat gattttcgcc gccaccgttg aacacgcaaa agagattgtg ggattactgc
    16081 ctgccgaaga tgctgcactg attactggcg acccccccgg cgctgagcgc gatgtgttaa
    16141 ttgaagattt taaagcccag cgttttcgct atctggtcaa cgtcgcagta ctgaccaccg
    16201 gatttgacgc cccgcacgtc gatcttatcg ccattctgcg ccctaccgaa tcagtgagtc
    16261 tttaccaaca aattgtcggg cgcggtctgc gtctcgctcc gggcaagact gattgtttaa
    16321 ttcttgatta tgcgggtaat cctcacgatc tctacgcgcc ggaagttggt acaccgaaag
    16381 gcaaaagtga caacgttccg gtacaggttt tctgccctgc ctgcggtttt gccaacacct
    16441 tttgggggaa aacgaccgcc gacgggacat tgattgaaca ctttggtcgc cgctgtcagg
    16501 gatggtttga agatgacgac ggtcatcgcg aacagtgtga cttccgtttc cgttttaaaa
    16561 attgcccgca atgtaacgca gaaaatgata ttgccgctcg ccgctgccgc gaatgtgaca
    16621 cggtactggt tgatccggat gatatgttaa aagcggcgct acgactgaaa gatgcgctgg
    16681 tattacgctg tagcggcatg tctttgcagc atgggcacga cgagaaaggc gaatggttga
    16741 aaatcaccta ttacgatgaa gacggcgcgg atgtgagtga gcgtttccgt ctgcaaacgc
    16801 ccgcccagcg taccgccttc gagcagcttt ttatccgccc gcatacgcgc acaccgggca
    16861 tccctctgcg ctggatcacc gccgccgata tcctcgccca gcaagcctta ctgcgacatc
    16921 cggattttgt cgtcgcccgc atgaaaggtc agtactggca agtgcgtgaa aaagtgttcg
    16981 attacgaagg tcgttttcgt cgggcgcacg aattacgcgg ttaatggtag ttttgattga
    17041 tgtataaggc gattgagtat agaatctcgc ccgcttttgc atacgcaaag cagatcactt
    17101 acctgttgct gggtcgcctg tagcaggatt aatttaagag agaaagaaat gtttactatc
    17161 aacgcagaag tacgtaaaga gcagggtaag ggtgcgagcc gccgcctgcg tgccgctaac
    17221 aagttcccgg caatcatcta cggtggcaaa gaagcaccgc tggctatcga gctggatcac
    17281 gacaaagtca tgaacatgca agctaaagct gaattctaca gcgaagttct gaccatcgtt
    17341 gttgacggta aagaaatcaa agttaaagct caggacgtac agcgtcaccc gtacaaaccg
    17401 aagctgcagc acatcgactt cgttcgcgct taattgctga ataagttgta aaaaaccccg
    17461 ctccggcggg gttttttgta tctgcagatt atgcctgatt acggtattgc tattttttgc
    17521 aggccagata aggcgttcac gccgcatctg gcattcagaa cacaaaaccg attaattgcc
    17581 gccagacgtc cggcgctgca attggtcgcg caaattcggc ggcgtgcctt taatggtcaa
    17641 ggtatcggtt gctggatccc agaagatgcg ttcacctaac agcatcgcat caaagttaat
    17701 agtcaagcca ccgccgctac cggcaaactt cgtcaactga cgcagtgtgc tacggtctgc
    17761 cgggaagctc tcttccagtt cgtagccttt ttcagccgca aactccgtga agctcacttc
    17821 gcttacgcct gccagctctt tcgacagcga ttccagttcg atctcttcgc ctgcctgcag
    17881 ctgctcatta cagtagctat aaacctgctg gcgcacgttc tgtcgctctg ctttatccag
    17941 ttgtgcttcg gcagtgaaat catcaaccgc ttgcaacagc ccacggtttt gcgctttcgc
    18001 gttcaatcct tcgctggccc cgaggaaatc cataaagaaa tccgccactt tgcgccctac
    18061 tcgccctttc aggaaagtga gatagcgggt ggactctgga ttggtttccc attcggttaa
    18121 atcgatacgc gcaacaatat ccgcatgatt gatatcaaga taatgggttg ggttgatgtc
    18181 gagattttcg ttaacgcgca tactgctcag gttgctcaga actgccacca gcagatactc
    18241 caccgccaga taacggtagt ggcaaaacag cacgaatcca ccgtcagcaa aaggatattt
    18301 cgctaattcg tcacgcaggc gaccggttgc cgcgcggcta aatgccagaa agtcctcttc
    18361 gccctgacgc tgtaagcgca gcgtctgcgc cagttcactc tcttcgctaa acaagccgta
    18421 agctttattt ttggcgctat agacccgatg cagttctgcc accatctcaa cgacggtttc
    18481 tgtcggttcc agcaatgaat cgcgcaacac cagctcaagg ttctgctcat cacgcttgat
    18541 aagctggtgc agagcaatct ggttgatatc cagactcatg ataaactctc ctttaagacc
    18601 gggcggtatt caaccaccgc cggttaagcg acgcaataaa agatggcggt cattttacag
    18661 aaggcagcgt aacgagaatg atttaacgca taaaaaagca gaaaaaaaac cgttgctacg
    18721 gtaatatgtt gccctttcat caactaactg atttcgattt atgccacaaa tttcccgcta
    18781 cagtaatgaa caggttgaac aattgctcgc agagctgctc aacgtactgg aaaaacataa
    18841 ggctccgacc gacctttccc tgatggtgtt aggcaacatg gtcactaacc ttatcaacac
    18901 cagcattgcc ccggcccaac gccaggcaat tgccaactct tttgcccgcg ccttacagtc
    18961 ctctatcaac gaagacaaag cgcactaagg gaaacagata acaggttatg gtaactcatc
    19021 gtcagcgcta ccgtgaaaaa gtctcccaga tggtcagttg ggggcactgg tttgcactgt
    19081 tcaatattct gctttcgctc gtcattggca gccgttacct gtttatcgcc gactggccga
    19141 caacgcttgc tggtcgcatt tattcctacg taagcattat cggccatttc agcttcctgg
    19201 tgttcgccac ctacttgctg atcctcttcc cgctgacctt tatcgtcggc tcccagaggc
    19261 tgatgaggtt tttgtccgtc attctggcga cggcgggaat gacgctatta ctgatcgata
    19321 gcgaagtctt tactcgtttc catctccatc ttaatcccat cgtctggcag ttggttatca
    19381 acccagacga aaatgagatg gcgcgcgact ggcagctgat gttcatcagc gtgccggtta
    19441 ttttattgct tgaactggtg tttgcgacgt ggagctggca aaagctgcgc agcctgacgc
    19501 gtcgtcgacg cttcgcgcgc ccgctggccg cattcttatt tatcgccttt atcgcctcgc
    19561 atgtggtgta tatctgggcc gatgccaact tctatcgccc gatcaccatg cagcgcgcta
    19621 acctgccgct ttcgtacccg atgacggcgc gacgtttcct tgagaagcat ggcctgcttg
    19681 atgcgcagga gtatcaacgc cgtcttattg agcaaggtaa tccagacacc gtttccgtac
    19741 agtatccgtt aagcgaactg cgctatcgcg atatgggcac cgggcagaat gtgctgttga
    19801 ttactgtcga tggcctgaac tactcacgct tcgagaagca gatgcctgcg ctggcaggtt
    19861 ttgctgagca aaatatttcg ttcacgcgcc atatgagctc cggcaacact acagacaacg
    19921 gcatctttgg cctgttctat ggcatctcgc cgagctatat ggacggcatt ctgtcgaccc
    19981 gtacgcctgc ggcattaatt actgcgctta atcagcaagg ctatcagctg gggttattct
    20041 catcagatgg ctttaccagc ccgctgtatc gccaggcatt gttgtcagat ttctcgatgc
    20101 cgagcgtacg cacccaatct gacgatcaga ccgccacgca gtggatcaac tggctgggcc
    20161 gctacgcaca agaagataac cgctggttct cgtgggtttc tttcaatggc actaacattg
    20221 acgacagcaa tcagcaggca tttgcacgga aatatagccg ggcggcaggc aatgtcgatg
    20281 accagatcaa acgcgtgctc aatgcactgc gtgattctgg caaactggac aatacggtgg
    20341 tgattatcac tgccggtcgg ggtattccac tgagcgaaga ggaagaaacc tttgactggt
    20401 cccacggtca tctgcaggtg ccattagtga ttcactggcc aggcacgccg gcgcagcgta
    20461 ttaatgctct gactgatcat accgatctga tgacgacgct gatgcaacgc ctgctgcatg
    20521 tcagcacacc tgccagcgaa tattcgcaag gtcaggattt gttcaaccct caacgccgtc
    20581 attactgggt tactgctgcg gataacgata cgctggcaat taccaccccg aaaaagacgc
    20641 tggtgctgaa caataacggt aaataccgca cttacaactt acgtggtgaa agagtgaaag
    20701 atgaaaaacc acagttaagt ttgttattgc aagtgctgac agacgagaag cgttttatcg
    20761 ctaactgatt aattataaat cagttagcga aatatcttac ttgcaatcgg tgtggaaaac
    20821 ggtagtatta gcagccacga gtcggcacgt agcgcagctt ggtagcgcac cgtcatgggg
    20881 tgtcgggggt cggaggttca aatcctctcg tgccgaccaa aaatcccaag aaaaaaccaa
    20941 cccttacggt tggttttttt atatctgcaa ttaattcgat aaacagaccg tgacacatca
    21001 cagcctgttt attttctgtt atcagaacgt ccagaccaca cccgcctgag tattccacgg
    21061 cgattctacc cctgcgccat tgccatagct gactgacaaa tgaccgctta acgtcggggt
    21121 aaacgatgac cttatcccag cctgataaac gccactggtt ccagccacat cattattaaa
    21181 tttgccatcg tcattaactt tcacctggtt agaatcggcg tattcctgac gcacggcggc
    21241 tttcagccag ggttccagcg tcgtaccgtt ttgcaggtcc atgtgatagc ttaccgccgt
    21301 tcccgcttca gcgcgtaata tccgggtatt tcccacatcc gcgcgcatgc cgtttgataa
    21361 cgtgtagtcc tgaccatctg tggtaaagcc ggtaaaggcc agatagggtc taacactcca
    21421 caatccgtca acccaacgga acccgctctc aacatgagca cccgcgccgt tactattgta
    21481 atcgccaaac gctgttgccc cattactcat cttgccatgg atggtgttgg caaaacggtc
    21541 aactttcacc accccatcaa cataggcacc gttctgatgc tcccaaccgg cataagcccc
    21601 cagggtatag ctatcgatat taccttttcc gccgcgatca aaaccaatat cagaatgaga
    21661 gtaaccaaag aacaagccgc gaattgtact gctttcttca cgggagaaac ggctatcgat
    21721 accgagcgtc aggcccgtca atgtttgctc aaaaccagct cccgcatcag tggtcacgtt
    21781 gttgcgggtg ttaattgccg aactccacat caccgtatcg taactaacgc cttttacgct
    21841 accaagacgc tcacgcacgg tgtccagttc tgcatcaaat accagcggtt gtgcggctgc
    21901 catgttcagc acatcggtgg ttgaaggggt aatttgcgcg cgattctctg cgaggctcca
    21961 gctatggttg ccattatcca gcaaggtata ttcatacgta ccgatatcaa caacgcctcc
    22021 ggcattgccc aacgtaaatg cagcatcacc gccgcccgtt gttaccagtg taaggctatc
    22081 tcctgctgcc gggctggcac cggtgtccgt cacgaatatt ttgaaatcac ctgttgcctg
    22141 accggtgacg ttgagctgat cgctctgatg attggccata tcggtacgca tataaaaatt
    22201 gccgtttccc gacagggtat tggtggtcaa cgtaatgaaa ttttccgcac ttgccgtcac
    22261 agatgaacga gtcactggct cagccatcat aatcacattt gcgccggaca tcgcaaggtt
    22321 ggctaagttg aggcgagtgt tttggttcgt cgtagtgata tcggcaatga tggtccatgc
    22381 gctattttcg agcgaaacgt ccgctttgct ggtatccacg gcaccatgcg ttctaaaaga
    22441 agcgccctca gaaaccacta ccttacccac caacatagcg ggtgcataat ttatttgcgg
    22501 cttcatgacc tcaagaatgc catcattccc tctgactgac acgttaacca ttgtgccagc
    22561 ccagacatta gcgcggccat tgttgattac catattttca gagatggctt ttgaggaata
    22621 cgtgatactt ccattctggt ctgatcgacc gagatcatat accgcatttg cctgcacttt
    22681 cgtaccagta tcctttccca gcgattgcat agtggcatgc tcatcaagga tagtgtcaat
    22741 agcctgcgtg tcctccataa caatgagccc ggaaccatca tccagttcat aattttttga
    22801 cacaccatcc tttatactaa attggccttt actgtttgta ccactcactt ccagcgcatt
    22861 cgttgagaca attaatttcc cgcctgcttt tttatcaacg ccagttgcag tcccgccatc
    22921 cataacctcc agtaagccgc cactatccac agtagtatta taagcgaagt cattttcttc
    22981 aactcgcaaa cttccgccgt tttccaacaa catattatta gcaataccat tcttgatatc
    23041 gaactgaccg agacggtttg ttccgaatac ctccatggcc cgcgtggttg ttttaatagc
    23101 accgcctgct ttctgatcta ccgcaagcgc aaatcccccc tctttaacct ccagtagtcc
    23161 tcccttttcg atagcggcaa aaacggtccc cccatctttt tcaacgatta agcgcccgcc
    23221 tttaatcgtg gtatcattcg ctgggccata aacctgtagc acaccattat tgagggtagt
    23281 ctggtttgcc gcgccaccca tttgaacaat ttgataacca tcatttaata ctgagtattc
    23341 tgcggtacca ttcatttcaa tgtattgata ccctccgtta atttcagtat tactacttac
    23401 accaaattct tttatatgct gttctccgcc aggattgatt ttcgtattat tggcggtccc
    23461 ataaaccagc tggatatctt tttccaggat aacaccatca actgtctcac catactcaac
    23521 tgttgatgcc aatgctatgg gtgaatataa cgctgtagcc accagaacag atattgcgga
    23581 acgacaaaga gaaacagaac cagattgatg cattgagctt tcatcctatg aaattaattg
    23641 cagttaaaag cattgggtac agaaaatacc catagctcca tacccggagt cagtttttaa
    23701 aaactgttta aagaaatgca caagtattgt gattgatttt ttagttgttt ttcttgatga
    23761 gaagctgatg caaaattccg tctttataat gaaaatgatg ccaaagcgaa cgacaaggtt
    23821 gtagttttca ctacatgtcc atacataaaa tggggtaaca ttcacgcgcc tggtagcgtt
    23881 accaacgcta cgctcaaaca taatgattct aataaaacct caggagacta ctatgcctga
    23941 agcaacgcct tttcaggtga tgattgtgga tgatcatcca cttatgcgac gcggtgttcg
    24001 tcagttactg gagcttgatc ctggctttga agtggtcgcc gaagcgggcg acggcgcgag
    24061 cgcgatcgat ctggcgaata gactggatat cgacgtgatc ttgctggatc tcaatatgaa
    24121 aggtatgagt ggcctggata ccctcaatgc cttgcgcagg gatggcgtta ccgcgcaaat
    24181 tattatcctg accgtatccg atgcctccag cgatgtcttt gcgctgatag acgcaggcgc
    24241 agacggttat ctgttgaaag acagcgaccc ggaagtattg ctggaagcga ttcgtaccgg
    24301 agcgaaaggc aacaaagtct ttagcgaacg cgtcaatcag tacttacgtg aacgtgaaat
    24361 gtttggcgcg gaagaagatc ccttcagcgt gctgacggag cgcgagctgg atgttctgca
    24421 cgagctggca caggggctgt caaataaaca gattgcatcg gtgttgaata tttccgagca
    24481 gacagtaaaa gtacatattc gcaatctgct gcgtaaactc aatgtccgct cacgcgtggc
    24541 ggccaccatt ctgttcctgc aacaacgcgg ggcacaataa aaatagcccg atggatttat
    24601 catcgggctg agatttatga caaacgcaaa actgcctgat gcgctacgct tatcaggcct
    24661 acgtggatcg atcaatttat tgaatttaca caattttgta ggccggataa ggcgttcacg
    24721 ccgcatccgg cataaactaa gcgcactttg tcatcagtct agcccgatgg catcaccatc
    24781 gggcctcttt ttatttactc tcctgcggcg acaaatgttg catcgcctgc gcgatactac
    24841 gttcaatcac cgcacggcga gtatcgttgg caggtaagag tttcaacatc atctcccacg
    24901 cggcaacggc ttcgccaaat cgctgctgct caaaggcatt aaacgcatac atgcttagca
    24961 cacggatatt gctatggtcc gttctcacca gctgacgtag cagttcaccg ccgaggcggt
    25021 tgtcgttggg atcagatgaa cgagtcaacg cttcagcgta tcccagtgcg gcatcgctgt
    25081 tcttcggatc gaggcgatac gcagtagcgt atgcatcggt ggcgatactg gcgttaccca
    25141 gcgccatgcc aacgcggccc aacataatcc agccttctat atctcccgga tttttttgca
    25201 gttgagtacg catccccagc gcaaggcgcg acatctcttc ttcgttgagc ggatcggctt
    25261 tcgggtccag cgccctgtcc agcaacgccg gagcctgtgc cgtggcctgc tgccagattt
    25321 tcacctgctg ataattgcca gtctggtagt agctgacgcc agccacaatt aacgccacca
    25381 caatacccgg cagataaaca acatatccgg cacgcttacc ttccggcacg ctttgttcag
    25441 gaaacgcttc cggcaccacg cgtacccgac gccgcgagcg ggcgtatatg acccaaccgc
    25501 caatgccaat agccactact ggcagcaccc acagcagcac ggtcagcggc gttaacggcg
    25561 gatcgtaagt gacgaagttg ccgtaacgcg ccaccatata atcgacaatc tcttttttac
    25621 ttttaccttc ctgcatcagt tcatacactt tctgacgcag gtcggtggca atcatcgagt
    25681 tggaatcggc aatgctgttg ttctggcatt tcgggcagcg cagttcttca gtgagctgac
    25741 ggaactgttg ttcctgtgct tcatctttaa actgcaacac gtcgatggtc gccagcgctg
    25801 agccggagat catcagcatc agcacgccca ataaaaacct cattgtgcgg cctccttact
    25861 gtatttctcc cacagcggct tgatctcttc ttcccagacg cgaggattga gatcgcccgc
    25921 atggcgatag cgaatgatac cgttgccgtc aataaggaag gtttctggcg cgccatagac
    25981 accgagatcc agccctaaca tgccgtcgcc atcaaacagg cttagcgcgt aaggattgcc
    26041 cagctctttc agccagctga ttgccttctg gcgatcgtct ttatagttca tgccgaccac
    26101 gcgaatgccc tgcgctgaaa gctgattcag atattgatgt tccgcacggc aggtcggaca
    26161 ccaagtcgcc cagacgttaa gcagtactgg tttgccctga gtcagcacat ccgcctgata
    26221 aaactgcccc ggattgtcca gtgattcgag acgaaacttc ggcacaggct tgccaatgag
    26281 cgccgattcc agattggtcg gatcatcccc ttcggcatta cgcgccagct gccacagcag
    26341 cgccgcggca atcgccagga agataatcaa cggaattaac aatactttgc gcttcatacg
    26401 gcctccggcg cagttttttg cggactcacg cgcttacgat agcgaggatc aaacagacac
    26461 agcagtccgc ccaacgccat catcagcccg cccgcccaaa tccagcgaac aaatggtttg
    26521 tagtaaagac gcacggccca cgcgccgttt tccagctctt caccgagcgc ggcgtacagg
    26581 tcacgcgtga tgccgccgtc aattgccgct tcggtcatca tcgacccggc agtgttgtaa
    26641 taacgttttt ccgcatacag caccgtttcc ggtttaccct ctcgcgttac gccaatagtc
    26701 gccacaccgc cgcgccagtt cgggccagtc acctctttga catcacggaa ggtgaagcga
    26761 tattcatgaa tatcgacgct atcgccggac ttcatgcgca catcacgctc aacgctataa
    26821 ttctggctaa aggcaatgcc aacaattgtc actgccagcc ccaggtgagc cgccaccatc
    26881 ccccaataac tgaaggtggt tttcgtgccg cgtgaaatac gcagcgcagc ttccgcagtt
    26941 gccagcaccg caatccagca ggccattgcc aggccgagca ccgtcatcgc cacaactttg
    27001 ctttcgaaca gccacggcaa cagcagagac agcaccagcg tagagatgaa ggcgataatc
    27061 aataaattgc ggattttacg tgggcgatcg cgcccccagc gcaccagagg accgacacca
    27121 agcagtagcg caaacggcac catcagccag gtaaacatgg tgttgaagaa cggttcgcca
    27181 atcgaaatac tgcccagtcc aagttgcttg tgcaccagcg gcagcagcgt ccccagcaac
    27241 accaccagca tcgcggcgac cagcaaaacg ttgttcgcta acagcaaaga ttcccgcgac
    27301 cacagcgcat tgtttacgcg tgagcgaact ttgtgtccac gcgcggcaaa cagcagcagc
    27361 gaaccgccaa tcaccagcac cataaaggcg aggataaaca taccgcgcgc cggatcagac
    27421 gcaaacgcgt gtaccgatac cagcacgccg gaacgcacga ggaaagtccc caacagacac
    27481 aacgagaagg cactgattgc cagcagtaat gtccacgctt tgaagctggc gcgttgttca
    27541 gtgaccgcca gtgagtgcat cagtgcagtc cccaccagcc acggcataaa cgaggcgttt
    27601 tctaccggat cccagaacca ccagccaccc cagccgagtt cgtaataggc ccatgcggaa
    27661 ccgagcacaa tgccgagcgt caggaaaatc cacgccgcca gcgtccacgg gcgagtaaaa
    27721 cgcgcataag tgctgtccag acgcccgctc agcaaagaag caatggcaaa agcaaacgcc
    27781 accgagaaac ccacgtaccc catataaagc agaggcggat ggaagatcag ccccggatcc
    27841 tgtaacagcg ggttaagatc gcgcccttca atcgggaagt tcggcagcgt gcgtgagaac
    27901 gggttagagg taaagagaat gaacagcaaa aagccgacgc tgaccatccc cattatcgcc
    27961 agtacacggg ccacaatatc cagcggaata cgctgactaa aaatcgccac cgcaaaggtc
    28021 cagccgctca tcagcagcac ccacagcaga agcgaacctt catgcgcgcc ccaggtagcc
    28081 gccacgcgat accacaccgg aagctgggta ttggagttgc tggcaacata ggtgacggta
    28141 aagtcgttga ccacgaaagc attgaccagc accagaaatg cgccagccac agacataaac
    28201 agcagccagg caaacaagcg ggaagacgcc atcatgcgcg catctccgcg cgccacgccc
    28261 catagcggat acacggacag cagcagcgca atccctaacg ccaagcacag cagcccgttt
    28321 ccaatttccg gcatcatgat gctgggtcct tataaacact cgccgggcga cggtggttag
    28381 cttccatcgc tttctcaact tctggcggcg tgtagttttc gtcgtgtttc gccagcactt
    28441 ctttcgcgag gatatgattg cctttttcca gctcgccctg caccacaacg ccctgccctt
    28501 cacggaacag atccggcaaa atgccttcgt aagagacatc cactgagcct tcagcatcgt
    28561 aaatggtgaa ggtcactttc agcgaattgg gatcgcgctg cacgctaccc ggcatcacca
    28621 tcccgccaac gcgcagacgc tgaccgactt ccggcatctg ctgagtttca cgcttgccgt
    28681 agagaatttc ccccggcgta taaaagagat cgatattcga gcgcagcgca tatagcacca
    28741 gaccgatagt cagcgccagc cctgccaaca cggcacaggc aatccacaag cggtttttac
    28801 ggcgaatatt catgcagcct cctgctgttg cgcagcacgt aaacgcgcct cacgcgcccg
    28861 ctgttgcgcc acaccacgca gaattgcgcg atgttgcatc accgagtgca cgaccaaaac
    28921 caccagcgga ataacggtca tcaccaccgc cagccagaca aaaaaggcgt aaccgcccat
    28981 tgcgaaaaat tcattccagg aagcaaatgc aggggtcatt tacggcctct tttcagtatc
    29041 agttcactca cccacggacg gcgtttttcc atcagcaaaa tcaaattacg catccgcatc
    29101 agcgtcagcg tggcagacag gagcaggaag ccaaaaatcg accagcgcag cggcgaacgc
    29161 atcgccggat cgatactttg ctgcatccgc gttgatccct gatgcagggt gttccaccac
    29221 tccacggagt aatgaataat cggcagattc accacgccaa tcagcaccag gatacctgcc
    29281 gcacggcccg ccagacggcg gtcgtcgaag gcgtgccaca gggcaatcac acccacatac
    29341 aaaaacagca gcaccagttc agaagtcaga cgtgcatccc atacccacca ggtgccccac
    29401 atcggttttc cccatgcaga gccggtaacc agggcaataa aggtaaacac ggcaccaatg
    29461 ggggccatcg ccgccaccgc caggttggcc attttcatct gccagacaag gccaataaac
    29521 gccgccaccg ccattgatgc ataaatgccc atcgaccaga tcgccgcagg cacatgcagg
    29581 tagataatgc ggtagctatt tccctgctga taatcagccg gagcaaagcc gaatccccag
    29641 atccagccga cggtaagcac gaccacactg gcaattgcca gccacggtat aaaccagcca
    29701 cagatttgat acagccgtgg tgggatcgcc agttgatgca gtgttttcca catagtttcg
    29761 ataccagact cgaacaaaaa tcagtaatcc agcgttattg aatgctgatt cgtaacgctg
    29821 ccgccgtcgc aaaaggactt aatgtcgcgg tgcctgccag caacgcgcct aaaattgcca
    29881 gatacccatc aacgggcaaa tgcatagaag ccgcgtccat cgcggcggtg gcaaagatga
    29941 gtaatgggat agtcagcggt aacaccagta tgctgagcag cacaccaccg cgcttaagtc
    30001 ccactgtcag cgccacgccc ggtgcaccga gaaagccaag cgtaggcgtt cccagcagca
    30061 gcgtcagcgc catcacttgc cagccataaa catccattcc cagtagcatt gctaccagtg
    30121 gcgaaaggat gagtaacggc agaccggtta ccatccagtg cgccatcacc ttcgccagca
    30181 caacggcggg caagggtaac ggcaacaaca tcaattgttc aagactgccg tcctgcaaat
    30241 cgtcacggaa cagtcgttcc agcgccagca aggatgaaag cagcgcagca acccagataa
    30301 tgcccggtgc aatacgcgcc agcagttgcg gctccggacc gatactgagc ggaaaaaggg
    30361 taattacaat caggaagaac cacagcgggt tggcgatttc ggcgctatgg cgaaacgcta
    30421 cgcgcagttc aagacggaaa atacgccaga acatcatgcg gccctcgttt gcgtcagtga
    30481 aatgcggcga attttacttt cagcaacgtt gagcggctgg tgggtagtca gaatcacaat
    30541 ccccccctgc tccgtatgct gcgccatacg ctgggtcaga cgatcgacgc cgttaacgtc
    30601 aatcgcggta aaaggctcgt cgaggatcca taatgtggca cgggtcaacc acagacgcgc
    30661 taaagcgacg cggcgttgtt gcccggcaga aagttgattt accggaatat cttcgaatcc
    30721 ggcaagcccg gcctgcgcca aggcttccag acattgtgcg gcatcgccat cgcgatgata
    30781 aaagtgcaga ttttctaacg ccgtcagccg ggttttgatc cccggctgat ggcctatcca
    30841 taacaggttt tgatggtagc tgtcgcgtac ctgatgcaag ggctgccctt gccagagaac
    30901 ctcgccagcg tcagggcgag acaaccccgt cagcaaacgg agaagcgttg tcttccccgc
    30961 gccgttgcta ccggtgattt gtacccactc tcctgcgttc agcgtaaatg acaagccact
    31021 aaataaggtt cgttcatccc gctcacaaag tacctctctg gcttcaagca tacccacgca
    31081 ataaccctgt taaaaacctg gctcgacttc acgcatatcg ggcagcttgt gcgcgatccc
    31141 tttatggcaa tcaatacagg tttgcccatc tttcaccgcc tggtcatgca tcttcgcggc
    31201 aaccgatttc tgggcggttg tatccatata ctcgaagttg tgacagttac ggcactcctg
    31261 cgagttattg tccttcatgc gccgccactc attctgtgcc atcgtcagac gatgagcttc
    31321 aaatttctgc ggcgtgtcaa taacgccaaa aattttacca tacagctctt tacttgcttt
    31381 gagcttgcgt atcatcttcg gcacaaactc gtgcggaacg tgacaatccg gacaggtcgc
    31441 acggacgccg ctacggttgt tgtagtgcac ggaatccatg tattcctgat acaccgtgtt
    31501 gcgcatttcg tggcagctaa tgcagaactc ttcggtattg gctttttcca tcccggtgtt
    31561 aaagcccccc cagaagacga tgccgccaac aaaaccgatc aacagcagcg tccccagcgc
    31621 cagatggctg ggggtacgcc accatttcca caggcgctta atcagaccag gcttacggtc
    31681 agaatttccc ataataacct cttatttccc gtaacctttt gatggggtaa aggtattccc
    31741 cacgattggc gcggtatcgg cctgcggtac gtgacattgc agacagaaat aacgacgcgg
    31801 agccacttcc gcgcccactt tgccgtcgct gtccataaag tgagtaggac tgatacgcgg
    31861 cgcgccagtg gtgcgatagc tttcgacacc gtggcactgc aagcagcgat tggtattggt
    31921 cgttacctga taaccttcaa cgctatgcgg gatcattggc ggctgattca catagttcag
    31981 cggcatccga tcctgctctt ttggcatccg aatggcccct tcctgcgtcc cggatacttc
    32041 cggcgactgg ctaaaatcga cgccattagc cgcccaaacc gcgccgctta ccaccagggc
    32101 cagcatcgcc gtccattgac acagcgcttt cttcaggtca tggcttttca tgatttcgct
    32161 cccgaactcc atcgtgtagt tattgtaaat acatcctcag aacagacatc cacgcagcga
    32221 ccgcacgtca tgcaatcgcg gctggtgacc tgcaccgggc tttgttcatc cagcaccggg
    32281 gcacgtagca catgcggttc cgggcaaaca tgaaaacaat ccatacagcg gttacatttc
    32341 tgccgatcgc tggccgcaac ggtaatcaca cctttgctac ccagcacgcc atacagcgcg
    32401 ccaaccggac aaatgtgccc gcaccagccg tgttcaacga ccagtagatc aaataaaaac
    32461 agcgcgagaa taagcagcgc gccgctgccg aagcccatta ccaggctacg ccccatcaga
    32521 gaaacggggt tgatccattc ccaaatgagc gtgccagtta aggctgatcc caccagaatg
    32581 accaccaaca gcacgtagcg aatgtggcga gggatcgtcg cagactgatt gaggtcaaag
    32641 cgcctgcgta accagttcgc taagtcggta atcgggttca gcgggcagac ccagctgcaa
    32701 aataatcgct ttcccgccaa ggcgtagagc acggtgataa tcaccgcacc ggtcaacgcc
    32761 accgtggcag gcagatgacc actggcaaga ctttgcagcg tcatcagcgg atcggtcagc
    32821 ggaacggtgt cgaataacag gctactgctg tagttgccgt gcaagatcca cacaccaaac
    32881 catggaccgc tcaaaaacat ccccagcacg aagaactgac aaaggcgacg taacaccagc
    32941 caacggtgac tgcgccacca gccttttttc tccagcgcct cgcgcccggc gtcacgttta
    33001 cgatttgcca ttgttcccct ccagccagcc gaagcggtaa tggtgaccta actccccttt
    33061 cgccagtgac agcggtaaca ccttgattgc cggttgttcc agcacgcata ctttttcgca
    33121 cttaccgcaa ccggtacagg cgtcgctgtg aaccgtcggc agaaagcggg cgtgcttacc
    33181 ggtacgcgtg ttgcgctcca gctccaaggt gatggcctca tcaattttcg ggcattcgcg
    33241 ataacaaaca tcgcagcgca gcccctgaaa gttgagacag ttttcctggt ccaccagtac
    33301 cgccagcccc atccgcgcgt cgtcgatcga ttcaatctca cgatccagcg caccgcttgg
    33361 gcacactttg gcgcacggaa tgtcctcaca catttcgcaa ggaatatccc gtgcgacaaa
    33421 atatggcgtg cccgccgaca gaccagaggc cagcgtcgcc agttttaagg tgtcgtaagg
    33481 gcaagcctga acacactgac cgcaacgcac acaggcactg gcaaaggcgt tctcgtttat
    33541 ggctccgggc gggcgcaacc gcacgccaga tgcgcgtgcg gtttgctgtt gtaaccccag
    33601 cgccacaccc acggcagcca gcccgcctgc tgtgcgaacg acatcgcgca gaaagcggcg
    33661 gcgaccattt tgaggtttcg ctgaccggga cataatggct tacaccttct ccagtttgac
    33721 cgcgcacttc ttgaagtccg tctctttcga gagcggatcg gtcgcatcca gcgtcagttt
    33781 gttaaccagc tgtgcggcgt cgaagaacgg catgtacacc aggccctgtg gcggacggtt
    33841 acgaccgcgc gtttcaacaa tcgagatcac ttcgccacgg cgagaaacca ctttcacttt
    33901 gtcgccacgg cgcagatcgc gcgctttcgc atccagcggg tgaataaaca agaccgcttc
    33961 cgggaaggcg cggtgcagtt ccggcacacg gcgagtcata ctgccggtgt gccagtgctc
    34021 cagaacgcgt ccggtagaga gccacaggtc gtactcttca tccggtgctt ccgccgccgg
    34081 ttcgaacggc agtgcgaaga tcaccgcttt gccatccggt ttaccgtaga acttatagcc
    34141 ttcgcccgct ttcacgtacg ggtcgttacc ttcgctgtaa cgccactgtg tttctttacc
    34201 attcaccacc ggccagcgca gaccgcgcgc tttgtggtag tcatcgaacg gtgccagatc
    34261 gtgaccgtga ccgcgaccaa accatgcgta ctcttcgaac agcccttttt gcagatagaa
    34321 gcccagctcg cgggattcat cgttcagctg atcttccgcc agttcggata ccgggaattt
    34381 gctcacttcg ggggtggcat acagaacttc gtacagcgtt ttgccacgca gttccggttt
    34441 cttcgccagc agctcttccg gccatacttc ttcagttttg aagcggcggg agaactggac
    34501 taactgccag agatccgatt tcgcttcgcc tggcgcctgt acctgctgac gccagaactg
    34561 agtacggcgt tcggcgttac cgtaagcgcc ctctttctct acccacattg cggtcggcag
    34621 gatcaagtcg gcggccagcg cactgactgt cggatacgga tcggagacga tgatgaagtt
    34681 gcgcggatcg cgccagcccg gcatacgctc ttcattgatg ttcggcccgg cctgcatgtt
    34741 gttggtacac atggtccagt aaacattcag cttgccgtct ttcagcgcac ggtcttgtgc
    34801 caccgcatga agaccgattt tcgccggaat ggtgccgctc gggatattcc acttcttctc
    34861 acagatatca cgatgtttct cgttagttac caccatgtcc gcaggcagac ggtgagcaaa
    34921 ggtgcccact tcacgcgcag taccacacgc agaaggttgc ccggtcaggg agaacggacc
    34981 gcaacccggc tgggaaattt tgccggtcag caggtgcagg ttgtagacca ggttgttggc
    35041 ccacacacca cgggtatgct ggttgaagcc catcgtccag taggagatga ctttcttgtt
    35101 cggatcggca tacagctgcg ccagttgttc taactggtct ttcggcacgc cggtcatttc
    35161 agcggttttt tccagcgtat actcagcaac gaaggctttg taatcttcaa agctcatcgg
    35221 ttcggaggcg tcagaacccg gattcttcgc tgctttttcc agcggatggg tcggacgtaa
    35281 accgtagccg atgtccgtcg cccctttgcg caggttaacg tgcttgctga agaagtcctg
    35341 atttatcgca ttgttttgaa tgatatagtt ggcgatgtag ttcaggatca ccaggtcaga
    35401 ttgcggcgta aagatgatgc cgttatccgc cagctcgaag ctacgatgct ggtaggtaga
    35461 aagcaccgcc acggtgacgt tctggttaga gagacgacgg ttagtgatgc gtgaccagag
    35521 gatcgggtgc atctccgcca tgttagcgcc ccacagcaca aacgcgtcag cctgctcgat
    35581 gtcgtcatag cagcccatcg gctcatccat accaaaggta cgcataaagc caactactgc
    35641 cgacgccata cagtgacgtg cgttcgggtc gatgttgttc gaacggaagc ccgctttgaa
    35701 cagcttggac gcggcataac cttcccagat agtccactga ccagaaccga acataccgat
    35761 cgattccggc cctttttctt tcatggcggt tttgaatttc tcttccatca catcgaaggc
    35821 ctgatcccag gtgattgggg taaattcgcc ttctttgtca tatttaccgt ttttcatacg
    35881 cagcagcggc tgcgtcaaac ggtctttacc gtacatgatc ttgggcagga aatagccctt
    35941 aatgcagttc aggccacggt taaccggtgc gtccgggtcg ccctgacagg ccaccacacg
    36001 cccctgctgc gttccgacca gaacgccgca accagtaccg cagaaacggc acggcgcttt
    36061 atcccattta atggcttcct gctgaccaac aacggcgcgg gcaacgcccg gcacgctgag
    36121 accggcagcc gccgcagcgg ccgcaacggc gttagctttc ataaagctac gacgactgag
    36181 tttcatggtg tttcctcacc ttgctcttcc tgctggtgat aaaccagcga caccgccagc
    36241 acgccctcta cgttgcgtac tgactcaatg gtttggatca gcgtttcgct gtcttctgct
    36301 tccaccacca caatcaactg accgctcggc gcgtcgctga cagcaacttc acagccggga
    36361 aaggcgttca gttgggtgct gatgtctgaa attcgttcgc ttttggcctg cacgaccagg
    36421 ctgcaaactt gccagttagt gtgcatggag atactccgca gttatggctg ataccggaca
    36481 gctggctgca catgctccac atccgttgca aagttggcta ttaagttgcg cctggtagat
    36541 cccggagagc gtcgggcgaa agataattgc cattggctca cagctatcct gacagcggcg
    36601 gcattcaact gactgatacg ccaggcacgc gtccccgatg gtgaactgca aatcccagac
    36661 cctggtgtgg cgcggagaaa atagcgattc ggggcaagcc tgcgcgcagg cgtaacagaa
    36721 gctgcactcg ttatttttga aattaacgct cggatagcca cccgtgccgc gttgcagaat
    36781 gttgttttca caagcattaa tacaggcgtc gcagcgggtg caatgggtca gaaaatgaga
    36841 ttcatcaccg gaccagggcg gacggatacc gttactggct ttgcgccagc gaccagtgag
    36901 tatgccccga cgggatgcat caatcttcac attgaccttc catcattaac gcgctctgaa
    36961 aattgagagc aaccaaatag accgcataat taataagcca tttttatagc cgctaagata
    37021 ttaaaggatg tgtcaaagat gcataccccg atcggggtaa aacctgtagc aggatcaaaa
    37081 gagtggcggg aagcgtggca aaaacgggct tttgctcaca tttcaaatgg ttataaacat
    37141 atttatatag cgattaattc accagagata tttctgcttg tttgctccct catcagaatt
    37201 taacactaaa agagcaggta aaattgtctg aatgttcttt aagttattca taaagcaaat
    37261 taataaatct gatgaatatg ttaaccttca gcgacatcat cggtgaaaac ctataaatga
    37321 agaaggaaag caaaaaaatg aagaccattc tacctgcagt attgtttgcc gctttcgcta
    37381 ccacttccgc ctgggcggca gaaagcgtcc agccactgga aaaaatcgcg ccttatccac
    37441 aagctgaaac aggcatgaag cgtcaggtga ttcagttaac cccgcaagaa gatgaatcta
    37501 ccctgaaagt agaactgtta atcggtcaga cgctggaagt cgattgcaat ttgcatcgtc
    37561 tcggcgggaa gctggaaagc aaaacgctgg aaggctgggg ctatgattat tatgtctttg
    37621 ataaagttag ctcgccggtt tccacgatga tggcctgccc ggatggcaag aaagagaaga
    37681 aatttgtcac cgcgtatctg ggcgatgctg gaatgctgcg ttacaacagc aagctgccga
    37741 tcgtggtgta tacgccagac aatgtagatg tgaaataccg cgtctggaag gcggaagaga
    37801 aaattgacaa cgcggaagtt cgctaaactg ttgtgaagta cggcaccccg ttggttggat
    37861 aaggcggtta cgccgcatcc aacatctaac gcccgagccg gttgcctgat gcgacgctgg
    37921 cgcgtcttat cagtatacgc cacatccggc ataccatgcc ggatgtggca catcattaca
    37981 acgcaatatc cgccacttct ttttgaacgg gttgcggttt caactgcggt ttcggcgtac
    38041 tatctgctgc ttgcggtttg tcgtatttca gccccagcac ttcgctggtg tactgcaact
    38101 cgcgttctgt tgccgctaca tcaccgttca gcttgcgtcc ataagacgga acgatcgctt
    38161 tcaacgtaga ctgccattgc gggctggaaa cgcgatcgcc aaatactttt tccagcagat
    38221 tcaacataat cggcgcggcg gttgacgccc ctggcgatgc ccccagcagc gccgcaatgg
    38281 tcccttgctg gtcactgacc acttcggtac ccagacgcag tacgccacct ttctcggcat
    38341 cacgcttgat aatctgcaca cgctgccccg cttgccacaa acgccagtcc tcttttttcg
    38401 cttgcggata gtactctttc aacgcttcaa aacgatcctc ttcactcaac atcacctgac
    38461 tcaccagata tttcaccaga tcgaaattat ccagcccgac gtgcatcatc ggcatcacgt
    38521 tagaggtggt ggtggaactc atcagatccc acaatgaacc gtttttgagg aatttggttg
    38581 agaaggtggc aaatggccca aacagcacta cgcgtttacc gtccagaaca cgggtatcga
    38641 tatgcggaac cgacatcggt ggtgcgccaa cggatgcttt accgtaaact ttcgccagat
    38701 ggcgattaac cacgtctgga ttttccgaaa caaggaactg tccgcccacc gggaaaccgg
    38761 cgtagtcttt cgcttccgga atccccgatt cctgtaacag cttcagcgcc gcaccgcccg
    38821 cgccgataaa gacaaatttg gtgcggatgt tctgtgcagt gccgtttttc agatcggcaa
    38881 cggtaacggt ccaggtgtta tcgtcattac gctttagggc gcggacttcg ctgctgagtt
    38941 gcagcgagaa gttagatttc ttctgtaagg aagcaattaa ctggcgggtg atctcaccat
    39001 agttcacatc agtgccaatt tctgttgcgg atcgcgccct tccatcacta acggtgccca
    39061 ctctttgatc tgcgcgtgat cttcagagta acgcataccg cgaaacagcg agctttgttg
    39121 caacgcggcg taacgggcgc gcaggaaatt aacgttatcc tcgccccaga caaagctcat
    39181 atgcggaacg gtattgataa atgaacgcgg agtacgcagc acgccgcgct cgacctggtg
    39241 cgcccagaac tggcgggaaa tctgaaatgc ttcgttaatg gcgactgctt tttcaatact
    39301 gatgctgcca tcggcgtttt gcggggtgta gttcagttcc atcagtgcag aatgcccggt
    39361 tccggcgttg ttccagccgt tcgaactctc ctgcgcgccc ccctccaggc gctccaccat
    39421 ggtcatcgac cattcaggct ccagctcgcg taaataggtc cccaacgtgg cgctcataat
    39481 gccgccgcca atcaacagta catcagtttc ctgctcctcg gacgctttcg cttttgccgc
    39541 catcgaaacg gcattaagcc ccacggccat cgagaagagc atggcagtca cttttttcat
    39601 cttgttaatg ccttactttt agtcgcttta ttgcaggtga gatttgcgcg gcatcaacgg
    39661 taacacttaa gtaacaacat ttaaataatg tttataaatt atattctaat tttagaaaca
    39721 ttataatttc gttgattaat tatagggtta ttagaaatga agggattttt agcatattga
    39781 cctgacggca gcagtccgcc aggtcagcgt cgtaatatta tgccgtccgg gcaacggcat
    39841 cacgcgaagc ggcatcgcgc tcttcgcccg tcagctcgct aagttgccca ttgcgcattt
    39901 ccagcaggcg gtcggcgtgg ataaagtaat gatcatcatg actgatagcg aaaatagttt
    39961 tacccatctc ctgcatcagc ggcagcaaca cctgataaaa ctcacgacgg aagtgtggat
    40021 cctgatccgc cgcccattca tccagcagga taatatcgcg ttcttctgcc agcgccagca
    40081 acagcggcac gcgttttttc tgcccttttg ataacttcag gttaacaata cgcccgttgc
    40141 ttaactcaag cttatgagcc attttcagct gcgccagcca cttctcaacc agttgcgggt
    40201 tagcgggttg cccctccggc cccagcagtt gatcaaacag ccagacatcg gtaaacactg
    40261 ccgaaaacag tttgcgataa tcttccggtt gttcgccgct gacaggtttg ccatccagca
    40321 agatttcgcc gctttgtggc tgatacaagc ccgtcaacaa catcgccagc gtcgattttc
    40381 cgctaccgtt gccgccaatc agaaacagca gctcgccacg tttgatggtg agattaatcg
    40441 gaccaacgga aaacgcgtta tcctgataag caaacgtcac gttacgcagc tccagcgttt
    40501 gccagttggg aaacgcctgc gggcgcggaa actcagcttt gaaaggcgcg agcgcgaatt
    40561 tgttcagctt gttaaacgcc acctgcgccg tcagcagcgt cggcaatgcg ccaaccgccg
    40621 aaagcagcgg cgtacgcagg aataacagcg tcaacgaata ggtcgcagca acgttggtat
    40681 cagcccaacc gagactgttc gccatccaga acaccaggcc gatagcgccc agcatcatga
    40741 tgtttgacca gttcacggca ctaagatgga aggtgtctgc gcgaataata tgatggcgat
    40801 actcttgcgc atcggggatg tagaggttgt taaacacata ctcggcgcgt tcccggttca
    40861 gagtcagctc tttgcgccct tcaagtacag tttgaaaatc cgtgtacagc ttgtcttcgg
    40921 tttcacgtag ggtcgccata tgtttgtaca cccgcgccac cagcacaaaa ccgccccaga
    40981 tggtgatcgc catccagata gccgttacca gcaacatttt gcccgacagc atccacagat
    41041 acgccgatga accgatagtg agaatgatcc cctgccccag ttccggcaga cgcacaaaag
    41101 caatggtgat attgcgcaca tcgctggtta atcccgccag caacgaggcg ctaccgagtt
    41161 gttcaatgcg ctcgacgtgg gtatccagaa tccgcttgat aaattcacta cgcagtcggt
    41221 aaacgaagtg atgccccaaa gtggtgagcg ccagttgcga tccgagagtg actgccatca
    41281 acagcagcaa taatcccaga aactccggta acaccagcag actggtatcc gccgtttcga
    41341 taaggcgctg attgataaaa gcaattaagc caatgcctaa tgccgcactg gcgaggctta
    41401 gcgccatcac actgataaat ggccagcgat actgccgcca gacaagtaca agaagttcca
    41461 tgcagaaaac ccggacaatg aattacagcc cgcagtttaa acatcttcgc gcgcacagca
    41521 ataataattc ttatttttat tcttttttac ctgcctgacg gaatgtcagg ttgtagcggc
    41581 agtcggtggt gagtggatga aaccccgctt tcaacggttg aataccgtga taaaacagcc
    41641 gcgattcacc gccccaggcc accaaattga aaaatcgcgg gtaagcccag agaaacagaa
    41701 acaattggcg cgcgcagatc cggttcgtct ttatcctgat gcagcgacag tttcgcgcta
    41761 ggagcgtagc ggttgataag acaagcatct ggctggaaat ctggatagcc cgccgccgta
    41821 gccgcacgtt gacataaatc atgaaagctt tgtggcatgg cgggccacgg tttatttgtt
    41881 tgcggatcaa tgggcgaata gagataacct tgccgatggg tcgtccagcc cagatgccca
    41941 cagttggtca tcgccaccga catggtatat ccgcctggag cgaccatctg gcgaaacggc
    42001 gactggctgg caacgtcatt aatatcgcgg attagttgct ccgcagcatt aaaagcaaaa
    42061 cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc acggttcagc atcggcaaac
    42121 agatccaaca ttacctctcc tcattttcag cttcgcggcg cagcagttgc gctttacgcg
    42181 acacgcccca gcggtaaccg gaaagtgtgc catcaccacg gaccactcga tgacagggta
    42241 ttacgatagc cagcttgttg gcggcacagg cgctggcaac ggcccgtacc gctttcggtt
    42301 tgccgatggc gttagccagt tgctgataac tgacggtttc accacaaggt atcgtgcgca
    42361 gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat atccagcggt aacgtcagcg
    42421 gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac atgttgctga aacgtcagat
    42481 cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa ctcgctgatt aatgtcgcgt
    42541 catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct ttctgccacc aggcaacgac
    42601 ccagctcaca atcagccagc gcgtaacgca ccgccagatt ttcgccaccg tgacggaatt
    42661 gtttagccgt catgcccagc gtttcatcgg ctttgcggta gtaactgctg ctgtcgggga
    42721 atccggcgtt aagaatagac gtcgtcacgc tctccccttt cgccagcgat tcgcgcaaac
    42781 ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat tccggtagta gctttaaaca
    42841 accgatgtag atgaaatgga ctcatcgcca cctggtcggc taaggcttcc agcgttacag
    42901 gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt atccaaccga tgttgccggg
    42961 gattggcttt gtctggctga caacgtttgc aggggcgaaa tccggcggcg agtgcctcgc
    43021 tggcatttgc gtagaaggag acattttccc gcaaggcatg tctggcgcgg caagacggac
    43081 ggcaaaagat acccgtggtg cgcacggcga aaacgaattc gccgtcggca tccgggtcgc
    43141 gggctaagac agattgccag cgttgatcgt cagttaagca tgtggcgttt ttcataatca
    43201 gctccctggt taaggatagc ctttagtctg cccggtcacc atcacgcaaa aaccaacaat
    43261 cttgcgcttt aatttttttc gctgacaagg aagcttttaa actgtggtga catccaggtt
    43321 ttaaagctat cgccttcttt ggtgatcata taaaccgcca gcccttcccg gcgaacaacc
    43381 tctttggctt tctccggccc gagtaccatc aagccagtat cccaggcatc ggcttccagc
    43441 gccgtcggag caatcaccgt cacggatacc agattgtgtt cgatgggacg cccggtttgc
    43501 ggatcgataa catgggaaag acgtttgccg tccagttcgt aatagttacg gtagctgcca
    43561 gaagtgctaa tcccatgacc gttgatatcc accacagcct gaaccgcgtt ttctttatcg
    43621 gttggttttt gaatcgctac ccgccacggc tggccttcac cgttcatacc acggctgttc
    43681 agcgcgccgc ccaccgacac cagatagcgg gaaatccctt cctgctccat caagcgtgcc
    43741 aggtgatccg ccgcataacc ttcaccgacg gtggagagat cgacatataa atccggcagg
    43801 tctttttgca gatattgctg atgcgactga ttgatgaccg tcaggtgctg taagccggtt
    43861 ttggctttca tcgcatcgat ctgttcctgg ctcggaattt gaaccggctg ttgttccggg
    43921 ccaaagcccc acagattcac cagcggcccg acggtaatat ccatcgcacc atcggtcctt
    43981 gccccaatgc gcagcgaggt ggtgacgata tcggccatcg cttcacttac cggccacggc
    44041 gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat cttttttata ggtcgaaagc
    44101 agctgatcgt cggcgtccag ctgggtctga atcttttctt taagttcggc actgcgtttg
    44161 gcatcaatgc ccgggatgct ggcacgccag aaggtaccca tggttttgcc ttcaagaacg
    44221 gtaacttcag tagcgtgggt tttggcgggt tgtggttttt gatcgcaacc aacaaagaag
    44281 agcgcggcaa ccagcagtgc cacgcgggta aagcttattt ccatacgtga ttatcctcat
    44341 gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta cgctgaaaac aatgaaaaaa
    44401 gggcccgcag gccctttgtt cgatatccat cgagattaga actggtaaac cagacccaga
    44461 gctacgatgt tatcagtgtt gatgccagcg tcacgagtga actggttgtc gtccagcagg
    44521 ttgattttgt agtcaacgta ggtggacatg tttttgttga agtagtaggt cgcaccaaca
    44581 tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac cattgatgac acccaggttt
    44641 ttacctttag actgcaggta agctagggac ggacgcagac cgaagtcgaa ctggtactga
    44701 gcaacagctt cgaagttctg tgctttgttc gcccaaccca agttgcctac gcgagttgcg
    44761 ttgtaggtct gggtgtactg agcagctagg tagatgttgt tcgcgtcgta tttcagacca
    44821 ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac gctcatcata acggttcaga
    44881 ccgaagttct ggtcatcagt acgtttggag ctggaaactg cagcaccgat accgaagcct
    44941 tcgtaatcat aagtaataga accgccaacg ccgtcaccgt tctgacgcag agcttcacga
    45001 ccgttgttgg tcatgccttc accttccggg ctaccgtttt tgccctgata ctgaacagcg
    45061 aagttcagac cgtcaaccag accaaagaag tcggtgctac ggtaggttgc gaagccgtta
    45121 ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac caccgaattc tggcagtacg
    45181 tcggtccagg aagttacgtc gtaaactacg ccgtagttac gaccgtagtc gaaagaacct
    45241 acatcctgga atttcagacc tgcgaatgcc acacgggtcc aggagttgtt ttcgttttca
    45301 gcgctgttgc cctggatctg atattcccac tggccgtaac cggtcagctg gtcagtaacc
    45361 tgagtttcac ctttgaagcc aagacgcatg taggtctggt cgccatctac agacttgtcg
    45421 tcagagaaat agtgcaggcc gtctacttta ccgtacagat ctaatttgtt gccgtctttg
    45481 ttgtaaactt cagcagcgtt tgctgctcct gctaccagca gagctgggac caggagggac
    45541 agtactttaa ctttcatgtt attaaccctc tgttatatgc ctttatttgc ttttttatgc
    45601 cactgcatac tgattaaccc tcattaatca gtcggcaagt ccattctccc caaaaatgca
    45661 gaataatcca acacgaatat gatactaaaa cttttaagat gtttcattta tcgctacaga
    45721 tgtttcaaaa tgtaaataca agggaacttt ttaagattat tgcggaatgg cgaaataagc
    45781 acctaacatc aagcaataat aattcaaggt taaaatcaat aacttattct taagtatttg
    45841 acagcactga atgtcaaaac aaaaccttca ctcgcaacta gaataactcc cgctatcatc
    45901 attaacttta tttattaccg tcattcattt ctgaatgtct gtttacccct atttcaaccg
    45961 gatgcctcgc attcggtttt tttacccttc tttacacact tttcattatt ctgtgctacc
    46021 acagaaaaac tataacgctt gttaactatt tcacaaataa ttaacatcag cataatttcc
    46081 agcaatcttt gtttatttac aattaatttc gttgggcttt ttgtaggtta tttgtacagc
    46141 aaaatggcgc ttgtacatct atttcccccc atacaggatg ataaatatca cgggagaata
    46201 gagaatcatc aatcaggtaa gagtctggaa tttcacactg taccctttat actgccctat
    46261 cacttcgcga agttttaaca ggtcataaac acgaatgcgt cagaaagaga caacggccac
    46321 gacccgcttt tcactcctac cggggagcat tacccgcttc tttttactgt tgatcattgt
    46381 gttactggtg acgatggggg taatggtaca aagtgccgtt aacgcctggc tgaaagataa
    46441 aagttaccag attgtcgaca ttacccacgc aatccaaaag cgcgtcgata cctggcgtta
    46501 cgtgaccagg cagatctacg acaacattgc cgcgacgacc tccccctcct ccggcgaagg
    46561 tttacaagag acgcgcctga aacaggatgt ctactatctg gagaaaccac gccgcaaaac
    46621 ggaagcgtta atctttggct ctcacgacaa ctcaacgctt gagatgactc agcggatgtc
    46681 cacttatctg gatacattgt ggggtgcaga aaatgtaccg tggtcgatgt attacctgaa
    46741 tggtcaggat aacagtctgg tgctgatctc aaccctaccc cttaaagatc tcacctccgg
    46801 atttaaagaa tcgaccgtca gtgacattgt tgattcacgt cgtgcagaaa tgttgcaaca
    46861 ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg cgccgcctgg cctggcagaa
    46921 cggtcattac tttaccttgc gtaccacgtt caaccagccg ggacatctgg caacggtcgt
    46981 ggcttttgat ctgccgatta atgatttgat cccaccgggt atgccgctgg acagtttccg
    47041 ccttgagcca gacgcgacgg caacgggaaa caatgataat gagaaagaag ggacggatag
    47101 cgtcagtatc cactttaaca gtacgaagat tgaaatctcc tcggcactca actctaccga
    47161 tatgcgcctg gtctggcagg ttccttatgg caccttattg ctggatacgt tgcaaaacat
    47221 tctgctgcca ctgctgctga acatcggttt gctggcgctg gcgttatttg gctataccac
    47281 attccgccat ttctccagcc gcagtacaga aagcgtcccc agcacggcgg tcaataacga
    47341 attgcgcatt ttacgggcaa ttaatgaaga gatagtctca ctgttgccgc tcggcctgct
    47401 ggttcacgat caggaatcga accgcactgt cataagtaac aaaattgccg atcatttgct
    47461 gccgcatctg aatctgcaaa acatcaccac catggcggaa cagcatcagg ggattattca
    47521 ggcgacgatc aataacgagc tgtatgagat ccgcatgttc cgcagccagg tcgcgccgcg
    47581 cacacaaatt ctcattattc gcgatcagga tcgcgaagtg ctggtaaaca agaaactcaa
    47641 gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg atgaccttta tgaaaaacat
    47701 tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag agtgcggcta aactcaacgc
    47761 cccggaaagc aaacaactgg cgaatcaggc agatgtgctg gtgcggctgg tcgaagaaat
    47821 acagttagcg aacatgcttg cggatgatag ctggaaaagt gagacggtgc tgttctccgt
    47881 gcaggattta attgatgaag ttgtgccttc agtgttgcct gccatcaagc gtaaaggtct
    47941 gcaactgctg attaacaatc atctgaaagc acacgatatg cgccgcggcg atcgcgatgc
    48001 cttacgacgt attttgctgc tactgatgca atatgccgtg acctcaacgc aattgggaaa
    48061 aatcaccctt gaggttgatc aggatgagtc ctctgaagac cgcctgacat tccgcattct
    48121 ggacacggga gaaggcgtaa gtattcatga aatggataat ttgcacttcc cgtttatcaa
    48181 ccagacccaa aacgatcgtt atggaaaggc ggacccgctg gcattctggc tgagcgatca
    48241 actggcacgt aaactgggcg gtcatttaaa catcaaaacg cgggatgggc ttggtacacg
    48301 ctactctgtg catatcaaaa tgctcgccgc tgacccggaa gttgaagagg aagaagagcg
    48361 tttactggat gatgtctgcg taatggtgga tgttacttcg gcagaaattc ggaatattgt
    48421 cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca cccgatgaaa gattaattag
    48481 tcaagattat gatatctttt taacggataa tccgtctaat cttactgcct ctggcttgct
    48541 tttaagcgat gatgagtctg gcgtacgaga aattgggcct ggtcaattgt gcgtcaactt
    48601 caatatgagc aacgctatgc aggaagcggt cttacaatta attgaagtgc aactggcgca
    48661 ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat gcgcaactcc atgccagcgg
    48721 ctattatgcg ctctttgtag acacagtacc ggatgatgtt aagaggctgt atactgaagc
    48781 agcaaccagt gactttgctg cgttagccca aacggctcat cgtcttaaag gcgtatttgc
    48841 catgctaaat ctggtacccg gcaagcagtt atgtgaaacg ctggaacatc tgattcgtga
    48901 gaaggatgtt ccaagaatag aaaaatacat cagcgacatt gacagttatg tcaagagctt
    48961 gctgtagcaa ggtagcctat tacatgaaca atatgaacgt aattattgcc gatgaccatc
    49021 cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat tgagtgggtg aatgttgtcg
    49081 gcgaatttga agactctaca gcactgatca acaacctgcc gaaactggat gcgcatgtgt
    49141 tgattaccga tctctccatg cctggcgata agtacggcga tggcattacc ttaatcaagt
    49201 acatcaagcg ccatttccca agcctgtcga tcattgttct gactatgaac aacaacccgg
    49261 cgattcttag tgcggtattg gatctggata tcgaagggat cgtgctgaaa caaggtgcac
    49321 cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg gaagaaattt accccggaaa
    49381 gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta cggtgacaag cgtctctcgc
    49441 caaaagagag tgaagttctg cgcctgtttg cggaaggcct cctagtgacc gagatcgcta
    49501 aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa gaaatctgcg atgatgaagc
    49561 tgggcgtcga gaacgatatc gccctgctga attatctctc ttctgtgacc ttaagtccgg
    49621 cagataaaga ctaatcatct gtaggccaga taagacgcgt tagtgtctta tctggcattt
    49681 gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc tacgattccc attatttcaa
    49741 cagattacat taaagtaggc cagataagac gcgtcagtgt cttatctggc atttgcactg
    49801 aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa tcccgcgatt tcctgaccct
    49861 ctcggcatat accgtcagcg tcttttttat cacatccagc gttaccggct tcgagaggca
    49921 gctgtccata ccggactcca gacaccgctg cttctcttca gccaacgcat tagcagttac
    49981 tccgattacc ggcaacgtca gtcccaactg acgaatgcgt tgcgtcaagc ggtaaccatc
    50041 catatttggc atgttgacgt cgctaagcac gatatcaata tgattcttac taagtacatt
    50101 aagcgcatcg acgccatcat tcgcggtttt acattgatag cccaacgatc ccaactgatc
    50161 tgccagcaga cgtcggttaa tcggatgatc atccacgacc agaatcatca tatcgtcgtt
    50221 atcgctgacc gctttgtccg tcgacggcag agcgttagca ggatcgtcgc tctccatctc
    50281 gatcaaataa atacgcgcca acaatgccgg tagctcatgc ggagcagcca cactgtgtac
    50341 ccactcccct ggcgctttct ccagcggaat accaatatgg cgacgacaga aggtcactac
    50401 cgctctgccc tgccattttt tactcactac ctcgtcagtg attaacacat cttcgggagt
    50461 cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg cgctgcaaac tggtttccag
    50521 gaactgacag agcgacgcat tgcggaccgc cagccagcag cgtttaccac tcaacccttc
    50581 cacgcctttt ttctgcgggt actgagcgcc gtacaacgga atacgcacgg taaactggct
    50641 gcccattccc ggttctgaat ctaccgagat atcgccgtcc atcatgctga tcagtttttc
    50701 acaaatcgcc agacccagac cggtcccctg gaagtttcgc tgtacgcccg ttccaacctg
    50761 gaagaaggga tcaaacaagc gcaccacttc tttcgccgga atccccacgc cggtatcgcg
    50821 aacacggata gagagataat cgccatcagc gcgaacatgc aaaactatac agccggtatc
    50881 ggtgaatttt atggcgttac tcaacaggtt ggagatgacc tgctgtaaac gcatcgggtc
    50941 gccatttaag gccactggca catccggttc aataaagcag tacaagccta actgcttgcg
    51001 taccaccagc ggtaaatagt tggcggtgat gtggttcatc acttcacgcg gtgaaaactc
    51061 acgcggttcg atcttcaact gttccgattc aatcttcgag aaatcgagaa tatcgctgat
    51121 aattttcaac aacaggctgg aagagttgtt cattgccgtc accagacgat cgacgccttt
    51181 cggtaactct ttggtttgca acagatccag gttaccgata atgccataca gcggcgttcg
    51241 cagctcatga ctgacggtgg caaggaacat cgattttgac tggctcgcct gttccgctgc
    51301 ttgtgccatc tcctgcaacg actcttccat cttcacgcgc gaagaaacat ccaccagcac
    51361 acaaatggcc acgttttcat tacgatagcg cgaatggacg aagctgattt gcagattggt
    51421 attgttgctg gtcaggacat caacaaaatt gacctgctgc ccacagataa tttgcgtcag
    51481 tcgttggcgg tcctcatgcg taagcatatt gagataggta tgcgccagtt cgttacttaa
    51541 aatattgacg ccatcagcgg tacgcaaaat gcagataccc actggcgcgg aggcgacaat
    51601 cttgcgattg aactgctcat gttcttccag tcgcagggcg tcgctttccg ccggaatgaa
    51661 aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca gctccggcaa gcacattcag
    51721 caaaattgcg ttaaggatca acatgcgaat gcgttccagc accttatcaa ccggcaccga
    51781 atacacgatg cttagcgatg agggtggcag atttttcttc agcaccagct cccggaaccc
    51841 ttccgtatag ccaaaccagg agcgttcctg catccagcga ggatcgccct taattttact
    51901 ttctggtccg gtaagcgaaa tcagggtatg accattttca tcaagaatgg taacccccat
    51961 cggcaacgta cccggtaaga aaaagttctc catccggatg gtctgctcga cacccaaaag
    52021 cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg taaaaatacc cgacgccagg
    52081 gcgcggacct tcgctgatcc agtagaggtt actgccgcta tcatcttgtg gtgcatttcg
    52141 atatttattg atgcgttcat gcaacgcttt caacgcggta tcgcgttcca ctggcatatc
    52201 acgcagaccg aaattggcca tgcagaggtt atcactgccg attaaaaata cccggttgag
    52261 atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata aaccacgcca atgactccag
    52321 agaacctcgc caggtgttac tcattgcgga acaatcggag tcggcaaaca gcggttcaaa
    52381 cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa agcacaccgt tttctgccga
    52441 taagcgattt tcggcgatgt acttaagctc tttcatcaca tcagaggtgc gttgaataaa
    52501 gcgctgagcc tgatcggaac tcagattaaa ttcctgacga atttccgatt ctcgctgatg
    52561 taacgcatta acgatgtaaa aaacggatga aaaagcaatc aacagccaga gcactaacgc
    52621 caatgctctg aacatgtagc gcgaggcttt cagggttgta cgaaaagaag caaggtattt
    52681 caaaggggcg aagctccgcc tcaggtgacc gatggagtgt ggttaaggta gcggtaaaag
    52741 cgtgttaccg caatgttctg tcaccattgc aataatgcgt atcgcttaga aaccattgcc
    52801 ttaaccagga aagccggagt agatggacag ttctcagtac gaaaaataag aaatgaccaa
    52861 tctgccgacg cttgttaatg gttatatcct ggcgtcggca gatcgcccag cagtcttatc
    52921 tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca ccagtcaaaa cttttcacca
    52981 gcgtcagctc gccagcatta cgcatcggta caataaatgt ttcctgtttc tcattgaccg
    53041 atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa ttccgtttcg ctgcgcccac
    53101 catagtagtt gatatacacc tgatagcgcc cgtgaactgg tgctggcatg gcgaaaatct
    53161 cgggtccgta ccccgtcgtg acatccatat ccagtgcacc actgtttttc agcacggtgt
    53221 taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg gagatcgaga tcggtattgt
    53281 ccatatccca cgagagaacc agccgtaaac gtgcacgaat agttcctgta cccggcgttg
    53341 agtaaaactg cattttttgc cggctttgcc catccgggct gatgacctgc acgctgttgc
    53401 tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc ggattcaatt ctctgcggca
    53461 tactggcacc gttgaccacc agccggccct gctgaacttt acccgccgca gcataattct
    53521 ttattttgcc gctgatctgc gctgagatat tttgatcatc tgccatgttg acggacgacg
    53581 ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc ctctgccgga tgccagccag
    53641 agagcggtgc gtcaatctcg acttcctgca caccttcact gtgagcgaca ggcgaaagtg
    53701 ccaccagtaa caacggaaga aaaatctttc gcatagcatt agtccattaa taattgtcgg
    53761 gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat aaccatcaaa ggccaagtgc
    53821 agatactcat gggttaaatc cagccgatcc tgaagcgtaa agaagttacg aataaacagc
    53881 cgtttctgct gccgatcggt ataggggaaa cctgagacta aacgacagac cgcaaacacg
    53941 tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc gccactgcgg catttttttc
    54001 gccagccagg ctttcgcttt gggtaataac tggcaggttg agcgtggagc tccccagcgg
    54061 ctaaggctgt tgtcgggata agcaaacgcg agtatctggt cgtaacgctc cccctgcccc
    54121 gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg tagcgcgact gccatgataa
    54181 tgaaccggat cgcctgcata gatgagctcc tgcgtccagg cggtcatcgt tcgcgccccc
    54241 gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc tgccggggat ggtcaggcaa
    54301 tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta tcgccacagt catggctttc
    54361 gctgcctcag gtggcgtact tttcgcctcg cgatccagaa ctctggcaac gtactcttca
    54421 cgatcgagat gcgactgcaa tttcagtttg cccttttcgc tcagtaaggt cgtctcaccg
    54481 tggctgacaa acgtaatgtg gttaccatta gtaaacgtca cacgatagcg cccattaagt
    54541 acgctgggtt ttactgccgt cgtactcttt tccgctgtga ttttcttcag cggatagcgg
    54601 gcaaacagct ccacctctac acattgccca ctggcaacct gcgtcggcac tggcaaaacc
    54661 cgattaagca ccgtcgcata acgggttagt accgttttgc tggtgccact gccagtaacc
    54721 cacagcggtg taccatcggt taaccagcca gcaaaaccac cctgacggat ttcctgttta
    54781 tcatcggcaa accagctcca ggttttcacc cgtacccgac taccgagcat ggatgagaca
    54841 ccgattttcg cctcgtccag caccacatcc agtaagactt cctgagcttt gttttgcgca
    54901 ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta ccgaggtttc tggcttcagc
    54961 gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt gccagtattg cccccacaca
    55021 tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc cacaggaacg taccagcgcg
    55081 gtatcgcgcg ttatgctctc cccagcctga caacaataaa cctcttccgg ggagtttccg
    55141 cgacactggt agggctgctc cggttgatgg gtatcctcca gccaggcgta aacatacagt
    55201 ttccacaaac tccctaacgg cgtggtgagc gagtcaggta atgctgagac tttggtcacg
    55261 cctgatgacg acagctgata cagttgatcg tgctgcgcac cgcgcagcac cagttgtaac
    55321 ggcgtctcct ccgcaagcgt tggcagagta accaacgcca atagccagac gattctgcgc
    55381 cagttcactt atttcacctg catcccggtc cattcactcc ctgccgcgac actttgctgc
    55441 gcaggtgcat aggaacgcac ataacgagca ggaggcagaa cgaattgccc tttttgcgag
    55501 aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt ctttcaccgg caccatatag
    55561 gccagttcgc ccatttcatt acgcgctttt tccagcaatt gcccctgctg ttccgcggcg
    55621 ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa cgtcggctcc cggcgggagc
    55681 ggtacttcca cctgaccata gcgcagaact gcatcctgct cgcaggtaag cgtaatttca
    55741 tcgagataca gcgcatcgct gtcaatctca ttgctggtca ccggctgcag agtaaagctc
    55801 atctcttctt caccggggat aagccgatac aactggcgtt caacggtcac cggaatgtta
    55861 ctttgttgag ccgtttttgc cggttcacgc cagcggacct gcacattttg cggcgataat
    55921 tcgtcaccaa aagagagaat gtccggcaca ccctgaccaa cccaacgcca gtcttcgccc
    55981 cctccagtta acttatgttt agcccatgcg cccgcaggcg caggcaaaac aactggaggc
    56041 attgtcgcca tatatttcgc cagccagttc atggccagcg cgcgctcaat agtggattgc
    56101 tcagcggtca aaccattaaa atagcggccg tctgggtagc gtcaccaccg ctattgagca
    56161 gcacaaccgt gtgcgccagc ggctgattgc tgtttgccgc tttatcccac gcatattgct
    56221 gaacctgtgg catcaccgtg gacttcaact gcgctttttt cagcaacgtc atggttaaga
    56281 cgtttgccac cgcatctgcc agtggagatt ccggtgtatc gaggatcagg ctgtcattga
    56341 tatcgcggat gtcttcctca gagaaatctt catctttagt tccgcgccgg gcgatagctt
    56401 catccccccc tttcaacaac gttttgcacg gcagattcat ctcctgtgcc cacgccagca
    56461 ccagcgcccg atgcaataac ggcatgttat ccgcctgctc ggcataactg tcgagcatat
    56521 gctgccagta ttccggttgt tgcgttacgc cgagcgcctg gctggcctgc caatcggcgt
    56581 accatgccca tgccgtaagg aaggcgtcac cattgccatc ttcaccccac caggtaaagc
    56641 gcgctccggg ccccgccagt tgcatcagcc gcagacggtt atcctgaatc atctgacgaa
    56701 tgtcgttagc ggcggcactt tgatgatcgg caagcgaacg ccaggcgaga ctgagcggga
    56761 tcagacggct accggtgttg atcaccccac cccacggttc atcgactaac gcatcaagat
    56821 tgttgcggaa aatctcctgc ggcgtttcac tactttgtag ccggatatta ctcgcctgct
    56881 cgggcaacat cagcgcgttc tcgccaccgc cgagcatgac atttttctgc tgttcaacgg
    56941 gccagctgtt gtccacaaaa gacagttttg tgctaatgct gtcctgcact tgcccatttt
    57001 gttgcagttc tgcacttaac aagccagatt gctgaatatt ctgcgtcagc gaaatataat
    57061 tcgccccttt gtgcagcgtc agcgtctggc gcatctcagc gcctgcaaat ttagtcacca
    57121 gcgccaccgg ttcgttatcc tgctgactga agataaacag tcctgccgcc ggtttgtcgc
    57181 ccacgcgata caccgttggc atactccact tcatgtagag atttttttcc gaacgcagat
    57241 aagcacgccc ctgcccgacc agcccgtcgc cgttcatccc acgcgcggtg atacgccagc
    57301 gggttaacga atcaggcatc aggaacgtga aatacgcttt gccttgttta tcggttgtga
    57361 gtgacggcat ccatgccgcg gtatccacct cttcacgccg tggacgttca ggcattttta
    57421 ctcgccgctc gctgcggtta gtcgcgccag gcgcaaccgg ctcgctggag agcgcctggt
    57481 catagctgat aaacgacaga ctggagctgg tacgcacatt gttacgcccc agcggataga
    57541 aaaatttgcc gatattcggc gcgatttctg gttgcagcgc gtagatcatt tcatcgacca
    57601 cgcctaccgt tagctgcgca gaaacaggtt tacctttcag cgacgaggtt aattctgaag
    57661 tggtcaacaa aaactggcca ccgagttaga gtttttccag tatcgatttt ccgattcgtt
    57721 tgggggtaac ccaccgttat attcgtgcgg tctta
//
LOCUS       DAJTYF010000012        56570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTYF010000012 DAJTYF010000000
VERSION     DAJTYF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.012"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..1014)
                     /gene="setA"
                     /locus_tag="OO590_000838"
     CDS             complement(<1..1014)
                     /gene="setA"
                     /locus_tag="OO590_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6636309.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAG"
     gene            complement(1116..1247)
                     /gene="sgrT"
                     /locus_tag="OO590_000839"
     CDS             complement(1116..1247)
                     /gene="sgrT"
                     /locus_tag="OO590_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6636310.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1336..2991
                     /gene="sgrR"
                     /locus_tag="OO590_000840"
     CDS             1336..2991
                     /gene="sgrR"
                     /locus_tag="OO590_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6636311.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3025..3124
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3155..4138
                     /gene="thiB"
                     /locus_tag="OO590_000841"
     CDS             3155..4138
                     /gene="thiB"
                     /locus_tag="OO590_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6636312.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPSGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4114..5724
                     /gene="thiP"
                     /locus_tag="OO590_000842"
     CDS             4114..5724
                     /gene="thiP"
                     /locus_tag="OO590_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6636313.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5708..6406
                     /gene="thiQ"
                     /locus_tag="OO590_000843"
     CDS             5708..6406
                     /gene="thiQ"
                     /locus_tag="OO590_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6636314.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(6520..7284)
                     /locus_tag="OO590_000844"
     CDS             complement(6520..7284)
                     /locus_tag="OO590_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6636315.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7370..8248)
                     /gene="araC"
                     /locus_tag="OO590_000845"
     CDS             complement(7370..8248)
                     /gene="araC"
                     /locus_tag="OO590_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6636316.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8587..10223
                     /gene="araB"
                     /locus_tag="OO590_000846"
                     /pseudo
     CDS             8587..10223
                     /gene="araB"
                     /locus_tag="OO590_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            10234..11736
                     /gene="araA"
                     /locus_tag="OO590_000847"
     CDS             10234..11736
                     /gene="araA"
                     /locus_tag="OO590_000847"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6636317.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            11936..12631
                     /gene="araD"
                     /locus_tag="OO590_000848"
     CDS             11936..12631
                     /gene="araD"
                     /locus_tag="OO590_000848"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6636318.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12706..15057
                     /gene="polB"
                     /locus_tag="OO590_000849"
     CDS             12706..15057
                     /gene="polB"
                     /locus_tag="OO590_000849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6636319.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHNPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15221..18127
                     /gene="rapA"
                     /locus_tag="OO590_000850"
     CDS             15221..18127
                     /gene="rapA"
                     /locus_tag="OO590_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6636320.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAILLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18139..18798
                     /gene="rluA"
                     /locus_tag="OO590_000851"
     CDS             18139..18798
                     /gene="rluA"
                     /locus_tag="OO590_000851"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6636321.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(18915..19730)
                     /gene="djlA"
                     /locus_tag="OO590_000852"
     CDS             complement(18915..19730)
                     /gene="djlA"
                     /locus_tag="OO590_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6636322.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            19985..22339
                     /gene="lptD"
                     /locus_tag="OO590_000853"
     CDS             19985..22339
                     /gene="lptD"
                     /locus_tag="OO590_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6636323.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22392..23678
                     /gene="surA"
                     /locus_tag="OO590_000854"
     CDS             22392..23678
                     /gene="surA"
                     /locus_tag="OO590_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6636324.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRANAYVKILSN"
     gene            23678..24667
                     /gene="pdxA"
                     /locus_tag="OO590_000855"
     CDS             23678..24667
                     /gene="pdxA"
                     /locus_tag="OO590_000855"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6636325.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24664..25485
                     /gene="rsmA"
                     /locus_tag="OO590_000856"
     CDS             24664..25485
                     /gene="rsmA"
                     /locus_tag="OO590_000856"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6636326.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25488..25865
                     /gene="apaG"
                     /locus_tag="OO590_000857"
     CDS             25488..25865
                     /gene="apaG"
                     /locus_tag="OO590_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6636327.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25872..26714
                     /gene="apaH"
                     /locus_tag="OO590_000858"
     CDS             25872..26714
                     /gene="apaH"
                     /locus_tag="OO590_000858"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6636328.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26792..27271)
                     /gene="folA"
                     /locus_tag="OO590_000859"
     CDS             complement(26792..27271)
                     /gene="folA"
                     /locus_tag="OO590_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6636329.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27462..29324)
                     /gene="kefC"
                     /locus_tag="OO590_000860"
     CDS             complement(27462..29324)
                     /gene="kefC"
                     /locus_tag="OO590_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6636330.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29317..29847)
                     /gene="kefF"
                     /locus_tag="OO590_000861"
     CDS             complement(29317..29847)
                     /gene="kefF"
                     /locus_tag="OO590_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6636331.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(29895..31289)
                     /locus_tag="OO590_000862"
     CDS             complement(29895..31289)
                     /locus_tag="OO590_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6636332.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(31348..31635)
                     /gene="fixX"
                     /locus_tag="OO590_000863"
     CDS             complement(31348..31635)
                     /gene="fixX"
                     /locus_tag="OO590_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6636333.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31632..32918)
                     /locus_tag="OO590_000864"
     CDS             complement(31632..32918)
                     /locus_tag="OO590_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6636334.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSLSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESSPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(32969..33910)
                     /locus_tag="OO590_000865"
     CDS             complement(32969..33910)
                     /locus_tag="OO590_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6636335.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(33925..34695)
                     /locus_tag="OO590_000866"
     CDS             complement(33925..34695)
                     /locus_tag="OO590_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6636336.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVNA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35166..36680
                     /gene="caiT"
                     /locus_tag="OO590_000867"
     CDS             35166..36680
                     /gene="caiT"
                     /locus_tag="OO590_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6636337.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFDIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36711..37853
                     /gene="caiA"
                     /locus_tag="OO590_000868"
     CDS             36711..37853
                     /gene="caiA"
                     /locus_tag="OO590_000868"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6636338.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            37982..39199
                     /gene="caiB"
                     /locus_tag="OO590_000869"
     CDS             37982..39199
                     /gene="caiB"
                     /locus_tag="OO590_000869"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR6636339.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            39273..40826
                     /gene="caiC"
                     /locus_tag="OO590_000870"
     CDS             39273..40826
                     /gene="caiC"
                     /locus_tag="OO590_000870"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6636340.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            40935..41720
                     /gene="caiD"
                     /locus_tag="OO590_000871"
     CDS             40935..41720
                     /gene="caiD"
                     /locus_tag="OO590_000871"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6636341.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41726..42316
                     /gene="caiE"
                     /locus_tag="OO590_000872"
     CDS             41726..42316
                     /gene="caiE"
                     /locus_tag="OO590_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6636342.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42402..42797)
                     /gene="caiF"
                     /locus_tag="OO590_000873"
     CDS             complement(42402..42797)
                     /gene="caiF"
                     /locus_tag="OO590_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6636343.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(43059..46280)
                     /gene="carB"
                     /locus_tag="OO590_000874"
     CDS             complement(43059..46280)
                     /gene="carB"
                     /locus_tag="OO590_000874"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6636344.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(46298..47446)
                     /gene="carA"
                     /locus_tag="OO590_000875"
     CDS             complement(46298..47446)
                     /gene="carA"
                     /locus_tag="OO590_000875"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6636345.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(47901..48722)
                     /gene="dapB"
                     /locus_tag="OO590_000876"
     CDS             complement(47901..48722)
                     /gene="dapB"
                     /locus_tag="OO590_000876"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6636346.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(48889..49803)
                     /gene="rihC"
                     /locus_tag="OO590_000877"
     CDS             complement(48889..49803)
                     /gene="rihC"
                     /locus_tag="OO590_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6636347.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(49869..50819)
                     /gene="ispH"
                     /locus_tag="OO590_000878"
                     /gene_synonym="lytB"
     CDS             complement(49869..50819)
                     /gene="ispH"
                     /locus_tag="OO590_000878"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6636348.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(50821..51270)
                     /gene="fkpB"
                     /locus_tag="OO590_000879"
     CDS             complement(50821..51270)
                     /gene="fkpB"
                     /locus_tag="OO590_000879"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6636349.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(51357..51851)
                     /gene="lspA"
                     /locus_tag="OO590_000880"
     CDS             complement(51357..51851)
                     /gene="lspA"
                     /locus_tag="OO590_000880"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6636350.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(51851..54667)
                     /gene="ileS"
                     /locus_tag="OO590_000881"
     CDS             complement(51851..54667)
                     /gene="ileS"
                     /locus_tag="OO590_000881"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6636351.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(54710..55651)
                     /gene="ribF"
                     /locus_tag="OO590_000882"
     CDS             complement(54710..55651)
                     /gene="ribF"
                     /locus_tag="OO590_000882"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6636352.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(55659..55877)
                     /locus_tag="OO590_000883"
     CDS             complement(55659..55877)
                     /locus_tag="OO590_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6636353.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            55980..56243
                     /gene="rpsT"
                     /locus_tag="OO590_000884"
     CDS             55980..56243
                     /gene="rpsT"
                     /locus_tag="OO590_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6636354.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        13545 a        15495 c        14680 g        12850 t
ORIGIN      
        1 ccccgctctt ccaggcatta aatcctgaaa ccatagcatc ccaatacccg caacaatgcc
       61 gataaataca gcgttaaaaa gttgcagcgt catcaacgcc atacggctat gaaagaaaat
      121 caatccagtg taaaacagca ctcctgccac cactgcaatg accatcattc gccgcttacc
      181 ataacgtttg acatagtagc cagccagaat cattgctggt atttccagtc cagctgccgt
      241 ccccatcagg aaacccgcca gtttgtctgg caatcctaaa tcgctactga tccacaacgg
      301 catatcaata atgtacatag tgttgcaggt ccacattaac gtcgaggcga caaataacat
      361 ccgtacgtta ctatcctgcc agtcaccttg cattgataaa gcattttccg acggcagttc
      421 tacccgcgcc acagacggaa gcataaatgc aatcaatacc agactgagtg tgaatatccc
      481 ggcggcaatc gaaaacatca ctgtaaagcc gtaattcaac gccagcataa aggccagcgg
      541 tggaccgatt acccatgcca gagaaagctg cgcacgcatc accgagctaa acatcaccac
      601 ttctcgcgcc gagttatccg cgtattcccg cgccagagca aataactgtg gcattgccgt
      661 attggccaga gatgccagaa gcacaccaca ggtgataagc gtcagataat gacgattaaa
      721 tgcaaacagt agcgcattgc caatagccat caaacagcaa aatataatca gttttcgccg
      781 atcgccctga ctgtcagaac gttttgccaa ccagaggctt acgccgatcc cagcaatagc
      841 attcaccgta taaaagagtc cgatccagaa aggttgcgcg ccaacctcac gactcagaaa
      901 taagctcaat gtaggagcct gtagcgcccc ggccaccccc atcataaaag cgaccagcat
      961 aaatgccgcg taaacaccgt tcatacggcg agccatcgtc attatccaga tcatacgttc
     1021 cctttttagc gcggcgagaa taaaaaaaac cagcaggtat aatctgctgg cgggtgattt
     1081 tacaccaata ctcagtcaca catgatgcag gcaagtcaac tttcagaatt gcggtcatcc
     1141 cactgcatca gtccttcaag catggttaat cgttgtggga cgcttaacca acgcaaccag
     1201 cacaacttcg ctgtcgcggt aaaatagtgc tgataaaact gacgcatggg gcatctcctt
     1261 gcttcatcgt taggaattat tgacttaata tagggaaaat aaaattgctg tcttttgcgc
     1321 aggagttccc cttttatgcc atctgctcgt ctgcaacaac agttcatccg cctgtggcaa
     1381 tgctgcgagg gtaaatcgca ggacacaacg ctcaacgaac tggcagcgtt attgagctgc
     1441 tcgcgtcgtc atatgcgtac cctgctcaac accatgcagg atcgcggctg gctgacgtgg
     1501 gaagcggaag tcgggcgcgg taaacgctcg cgtctgacat tcctctatac cgggctggcg
     1561 cttcagcaac agcgggcgga agacctgctg gagcaggatc gtatcgacca actagtgcag
     1621 ttggttggcg acaaagcgac tgtgcggcaa atgctggttt ctcatctggg ccgcagcttc
     1681 cgccaggggc ggcacatcct gcgcgtgctc tactatcgtc cgttgcgtaa tctgctacct
     1741 ggcagcgcat tgcgccgttc cgaaacccat attgcccggc aaatcttcag ttcgctaacg
     1801 cgcataaatg aggaaaatgg ggaactggaa gcagacatcg cccaccactg gcagcaaata
     1861 tcaccgcttc actggcgttt ctttttgcgt ccaggagtcc attttcatca tggtcgtgaa
     1921 ctggaaatgg acgacgtgat cgcctcttta aaacgaatca atacgctgcc gctctattcg
     1981 catattacgg acattgtgtc gccgacgccc tggacgctgg atatccatct cacgcagccg
     2041 gaccgctggt tgccgttact gctggggcaa gttccggcga tgatcctgcc gtgcgaatgg
     2101 gaaaccctca gtaactttgc cagccatcct attggcaccg gtccgtatgc ggtgattcgc
     2161 aacagcacca atcaactgaa aattcaggca ttcgatgact tcttcggtta ccgggcatta
     2221 atcgacgaag ttaacgtctg ggttctgccg gaaattgccg acgagccagc cggtgatctg
     2281 atgctaaaag gtccacaggg cgaggaaaaa gagattgaaa gccgcctgga ggaaggttgc
     2341 tactatttac tgttcgacag ccgcacccat cgcggggcga atcagcaagt cagggactgg
     2401 gtaagctatg tgctttctcc aactaatctg gtctatttcg ctgaggaaca gtaccagcaa
     2461 ctgtggttcc cggcttatgg actgctcccc cgttggcacc atgcccgcac cataaagagc
     2521 gaaaaaccgg ctggcctgga aagcctcacc ctgacctttt atcaggatca cagtgagcat
     2581 cgggtgattg ccgggatcat gcagcagatt ctggcaagtc accaggtcac gctggaaatc
     2641 aaagagatca gctacgatca gtggcatgaa ggagagatcg agagcgatat ctggcttaat
     2701 agcgccaact ttacgctgcc gctggatttt tcgctgttcg cgcacttgtg cgaggtgccg
     2761 ctgctccaac actgtattcc gatcgactgg caagccgacg ccgcccgctg gcgcaatggc
     2821 gaaatgaacc tggcgaactg gtgccagcaa ctggtcgcca gtaaagcaat ggtgccgctt
     2881 atccaccact ggctgatcat tcagggacaa cgcagtatgc gcggcctgcg tatgaatacc
     2941 ctcggctggt tcgattttaa atcagcgtgg tttgcgccac cggatccatg attgctggta
     3001 gataacaaaa tcactacact aacgccgttc tcaacggggt gccacgcaaa cgcgtgcgct
     3061 gagaaaatac ccgtcgaacc tgatccggat aacgccggcg aagggatttg aggctccttc
     3121 tcaagtcctt tgccactctt ttttgaggtg caaagtgtta aaaaaatgtc ttcccctgct
     3181 gttgctgtgc acagcgcccg ttttcgctaa acccgttctg actgtttata cctacgattc
     3241 cttcgccgcc gactgggggc ctggtccaaa gatcaaaaaa gcttttgaag ccgactgtaa
     3301 ctgcgaactg aaactggtgg cgctggaaga tggcgtttcg cttctcaacc gtctacggat
     3361 ggaaggcaaa aatagtaaag ccgatgtggt gctggggctg gataacaatc tgttagacgc
     3421 cgccagtaaa accggactat ttgccaaaag cggtgtggca gcggatgccg ttaacgttcc
     3481 cagcggctgg aataatgaca ctttcgtccc gtttgattac ggctatttcg ccttcgtcta
     3541 tgacaagaac aaactgaaaa acccgccgca aagcctgaaa gaattggttg agagcgatca
     3601 gaactggcgg gtgatttatc aggatccgcg taccagcacg ccggggctgg gtctgctgtt
     3661 gtggatgcaa aaagtctatg gcgatgacgc cccacaagcc tggcaaaaac tggcgaagaa
     3721 aaccgtcacg gtcaccaaag gctggagcga agcctacggc ctgtttttaa aaggtgaaag
     3781 cgatctggta ctgagttaca ccacctctcc ggcttatcac attctcgaag agaaaaaaga
     3841 taactacgcc gccgcgaact ttagcgaagg tcactatctg caggtggaag tcgccgcccg
     3901 caccgctgcc agcaagcagc cggagctggc gcaaaaattc ctccagttta tggtttctcc
     3961 ggcttttcag aatgcgatcc caaccggtaa ctggatgtat ccggtggcaa atgtcacgct
     4021 gcctgccggg tttgaacaat tgaccaaacc ggccaccacg ctggagttca caccagctga
     4081 agtggcggca caacgtcagg catggattag cgaatggcaa cgcgccgtca gccgttaatt
     4141 cccggctggt taattccagg tgtaagcgcc gccacgctgg tggtagcggt tgcgctcgcg
     4201 gcgtttctcg ccctgtggtg gaacgcgccg cagggtaact gggtggcagt ctggcaggac
     4261 agctacctgt ggcatgtggt gcgcttctcc ttctggcagg cgtttctctc ggcgctactc
     4321 tctgtcgtac ccgcgatatt cctcgcccgc gccctctatc gcaggcgctt tccgggtcgg
     4381 ctggcgctgt tgcgtctgtg cgcaatgacc ttgatcctcc cggtgctagt tgctgttttc
     4441 ggcattctta gcgtctatgg tcgtcagggc tggctggcat cgctctgcca atcgctcggt
     4501 ctggagtgga ccttttcgcc ctacggcctg caaggcattt tgctggcgca cgtatttttt
     4561 aatctgccga tggcgagccg cttattactc caggcactgg aaaacattcc cggcgaacaa
     4621 cgtcagcttg ccgcccagct tgggatgcgc ggctggcatt ttttccgctt cgtcgaatgg
     4681 ccgtggctac ggcgacaaat cccgccggtt gccgcgctta tctttatgct ctgtttcgcc
     4741 agcttcgcca ccgtgctatc gctgggcggc ggtccgcagg caaccactat cgagctggct
     4801 atttatcagg cgctgagtta cgactacgat cctgcccgcg cggcgatgct ggcgctgctc
     4861 cagatggtgt gttgccttgg gctggtgctg ttgagtcagc gactaagtaa ggccattgcg
     4921 cccggcacca cgctgctgca aggctggcgc gatccggacg atcgtctgca tagccgcatt
     4981 tgcgacacgg tgttaatagt gctggcgctg ctgctgttgc tgccaccgtt gctggcggtg
     5041 atcgtcgatg gggtaaatcg ccagttgccg gaagtgctgg cacaaccggt gctgtggcag
     5101 gcgctgtgga cctcgttgcg tattgcgctg gcggcaggtg tattgtgcgt agtgctgacc
     5161 atgatgctgc tatggagcag tcgcgaactg cgggcgcggc agaaaatgct ggcgggtcag
     5221 gcgctggaga tgagcggcat gttgatcctc gccatgccgg ggattgtgct ggctaccggc
     5281 ttctttttac tgctcaacaa cactatcggc ctgccacaat ctgctgacgg cattgtgatt
     5341 ttcaccaatg cgttaatggc gatcccttat gcgctgaaag tactggaaaa cccgatgcgc
     5401 gatatcaccg cccgctacag tatgttgtgt cagtcgctgg gcattgaagg ctggtcgcgc
     5461 ttaaaagtgg tcgagctgcg cgccctgaaa cgtccactgg cgcaggcgct ggcttttgcc
     5521 tgcgtgctgt cgattggtga ttttggcgta gtggcgttgt tcggtaacga tgatttccgt
     5581 accctgccgt tttatctcta ccagcaaatt ggctcctatc gcagccagga cggcgcggtc
     5641 accgcgttaa ttctgctact actctgtttt ctgctgttta ccgtgattga aaaactaccg
     5701 gggcgaaatg ttaaaactga ctgatatcac ctggctttat caccatttgc cgatgcgttt
     5761 tagcttaacg gtagaacgcg gcgagcaggt ggcaatcctc gggccaagcg gcgcgggtaa
     5821 aagtaccctg ctgaatttga ttgccggttt tctgacgcca gccagcggtt cgctgactat
     5881 cgatggcgtt gatcacacca cgacgccgcc gtcacgccgt ccggtgtcga tgctgtttca
     5941 ggagaacaac ctgttcagcc acctgacggt cgcgcagaac atcgggctgg ggctaaatcc
     6001 ggggttgaaa ctgaacgcgg cacagcagga gaaaatgcac gcgattgccc gtcagatggg
     6061 gattgataat ttaatggcgc ggttaccggg cgagctttcc ggcggtcagc gacagcgcgt
     6121 ggcgttagcg cgttgtctgg tgcgcgaaca gccgatttta ttgctcgatg aaccgttctc
     6181 tgcgctcgat ccggcgttac gtcaggagat gctgacgctg gtgagcacga gctgccagca
     6241 gcaaaaaatg acgctattga tggtgtcgca cagtgtggaa gatgcggcgc ggatcgccac
     6301 acgctcggtg gtggtcgccg acgggcgcat cgcctggcag ggtaagactg aagaattgtt
     6361 gagcggtaag gcgagtgctt cggcaatatt ggggattacg ggttagtatg cactgtcaga
     6421 tgcggcgtga acaccttatc cgacctacgg acagtgcgtt ttgtaggcct gataagacgc
     6481 ggtcagcgtc gcatgaggca acccgcacaa gacaatcccc taaaccccaa ccactttacg
     6541 caaaatatcg atatacaccg gcatcaacgg gtggcgaatt aacaccacca gcgccaccac
     6601 gccgatggca gaaatcaacg gcgtcagcca caacaaacgg ccgcgcgaca aataatgact
     6661 taaacgatcg gttgctttac cgctgcgcca taaccgccag cacagccagc caccaaccca
     6721 caaaaatacc gccgtcgcca gcagcaacca tttaaactcg ccgctctgca ttccggctgg
     6781 aatatcgatc gccgcgcccg ccagaatccc aggcaggaag taaaacggtg gccacagcag
     6841 gcagccgata atattcggcg taatgaattt tgccaccggc agatccagca ttcccgccac
     6901 cattggcacc agcggacgcg tcgggccaac aaagcgaccg accagaatgg tgaacatgct
     6961 gtgttgatgc aacgcatgtt cagttttatc gagtaatgct ttgtttttct tcagaaatga
     7021 ccagcgatgc aacggctttt taaaacgcca gcccagccag aaggagatcc agtcgcccag
     7081 cagacaaccg acaatccctg ccagccaagc gtgccagaaa cttaactcgc cgctgccaat
     7141 cagcgctccc agccccgcca tcagcaccgt accgggtaga atcaaaccga ccagcgccag
     7201 cgactccaga aaggccacca acaccaccgc catcaatgaa tacacggtag attgggtaat
     7261 aaagtgttcc agcaatgctt gcataatgtg cctgtcaaat ggacgaagca gggattctgc
     7321 aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact
     7381 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
     7441 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
     7501 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
     7561 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
     7621 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
     7681 ggtgatcgct gatgtactga caagcctcgc gtacacgatt atccatcggt ggatggagcg
     7741 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
     7801 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
     7861 aatacggctg gtgcgcttca tccgggcgaa agaaacccgt attggcaaat attgacggcc
     7921 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
     7981 cgcgagcctc cggatgacaa ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
     8041 cacccggtcg gcagacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
     8101 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
     8161 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
     8221 gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa
     8281 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
     8341 taacccaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca
     8401 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
     8461 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatccta
     8521 cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt tggatggagt
     8581 gaaacgatgg cgattgcaat tggcctcgat tttggcagtg attctgtgcg agctttggcg
     8641 gtggactgcg ccagcggtga agagatcgcc accagcgtag agtggtatcc ccgttggcaa
     8701 aaagggcaat tttgtgatgc cccgaataac cagttccgtc atcatccgcg tgactacatt
     8761 gagtcaatgg aagcggcact gaaaaccgtg cttgcagagc ttagcgtcga acagcgcgca
     8821 gctgtggtcg ggattggcgt tgacagtacc ggctcgacgc ccgcaccgat tgatgccgac
     8881 ggtaacgtgc tggcgctgcg cccggagttt gccgaaaacc cgaacgcgat gttcgtattg
     8941 tggaaagacc acactgcggt tgaagaagcg gaagagatta cccgtttgtg ccacgcgccg
     9001 ggcaacgttg actactcccg ctatattggc ggtatttatt ccagcgaatg gttctgggca
     9061 aaaatcctgc atgtgactcg ccaggacagc gccgtggcgc aatctgccgc atcgtggatt
     9121 gagctgtgcg actgggtgcc agctctgctt tccggtacca cccgcccgca ggatattcgt
     9181 cgcggacgtt gcagcgccgg gcataaatct ctgtggcacg aaagctgggg cggcttgccg
     9241 ccagccagtt tctttgatga gctggacccg atcctcaatc gccatttgcc ttccccgctg
     9301 ttcactgaca cctggactgc cgatattccg gtgggcacct tatgcccgga atgggcgcag
     9361 ttggcgcagg cgcacagcct aacgcactgg taaaagttat cggtacttcc acctgcgaca
     9421 ttctgattgc cgacaaacag agcgttggcg agcgggcagt taaaggtatt tgcggtcagg
     9481 ttgatggcag cgtggtgcct ggatttatcg gtctggaagc aggccaatcg gcgtttggtg
     9541 atatctacgc ctggtttggt cgcgtactcg gctggccgct ggaacagctt gccgcccagc
     9601 atgcggaact gaaagcgcaa atcaacgcca gccagaaaca actgcttccg gcgctgaccg
     9661 aagcatgggc caaaaatccg tctctggatc acctgccggt ggtgctcgac tggtttaacg
     9721 gtcgtcgcac gccaaacgct aaccaacgcc tgaaaggggt gattaccgat cttaacctcg
     9781 ctaccgacac tccgctgctg ttcggcggtt tgattgctgc caccgccttt ggcgcacgcg
     9841 caatcatgga gtgctttacc gatcagggga tcgccgtcaa taacgtgatg gcgctgggcg
     9901 gcatcgcgcg gaaaaaccag gtcattatgc aggcctgctg cgacgtgctg aatcgcccgc
     9961 tgcaaattgt tgcctctgac cagtgctgtg cgctcggtgc ggcgattttt gctgccgtcg
    10021 ccgcgaaagt gcacgcagac atcccatcag cccagcaaaa aatggccagt gcggtagaga
    10081 aaaccctgca accgcgcagc gaacaggcac aacgctttga acagctttat cgccgctatc
    10141 agcaatgggc gatgagcgcc gaacaacact atcttccaac ttccgccccg gcacaggctg
    10201 cccaggccgt tgcgactcta taaggacacg ataatgacga tttttgataa ttatgaagtg
    10261 tggtttgtca ttggcagcca gcatctgtat ggcccggaaa ccctgcgtca ggtcacccaa
    10321 catgccgagc acgttgttaa tgcgctgaat acggaagcga aactgccctg caaactggtg
    10381 ttgaaaccgc tgggcaccac gccggatgaa atcaccgcta tttgccgcga cgcgaattac
    10441 gacgatcgtt gcgctggtct ggtggtgtgg ctgcacacct tctccccggc caaaatgtgg
    10501 atcaacggcc tgaccatgct caacaaaccg ttgctgcaat tccacaccca gttcaacgcg
    10561 gcgctgccgt gggacagtat cgatatggac tttatgaacc tgaaccagac tgcacatggc
    10621 ggtcgcgagt tcggcttcat tggcgcgcgt atgcgtcagc aacatgccgt cgttaccggt
    10681 cactggcagg ataaacaagc ccatgagcgt atcggctcct ggatgcgtca ggcggtctct
    10741 aaacaggata cccgtcatct gaaagtctgc cgatttggcg ataacatgcg tgaagtggcg
    10801 gtcaccgatg gcgataaagt tgccgcacag atcaagttcg gtttctccgt caatacctgg
    10861 gcggttggcg atctggtgca ggtggtgaac tccatcagcg acggcgatgt taacgcgctg
    10921 gtcgatgagt acgaaagctg ctacaccatg acgcctgcca cacaaatcca cggcgaaaaa
    10981 cgacagaacg tgctggaagc ggcgcgtatt gagctgggga tgaagcgttt cctggaacaa
    11041 ggtggcttcc acgcgttcac caccaccttt gaagatttgc acggtctgaa acagcttcca
    11101 ggtctgcccg tacagcgtct gatgcagcag ggttacggct ttgcgggcga aggcgactgg
    11161 aaaaccgccg ccctgcttcg catcatgaag gtgatgtcaa ccggtctgca gggcggcacc
    11221 tcctttatgg aggactacac ctatcacttc gagaaaggta atgacctggt gctcggctcc
    11281 catatgctgg aagtctgccc gtcgattgcc gtagaagaga aaccgatcct cgacgttcag
    11341 catctcggta ttggtggtaa ggacgatcct gcccgactga tcttcaatac ccaaaccggt
    11401 ccagcgattg tcgccagcct gattgatctc ggcgatcgtt accgtctgct ggttaactgt
    11461 atcgacacgg tgaaaacacc gcactccctg ccgaaactgc cggtggctaa tgcactgtgg
    11521 aaagcgcaac cggatctgcc aactgcttcc gaagcgtgga tcctcgctgg tggcgcgcac
    11581 cataccgtct tcagccatgc gctgaacctc aacgatatgc gccagttcgc cgagatgcac
    11641 gacattgaaa tcacggtgat tgataacgac acccgcctgc cagcgtttaa agacgcactg
    11701 cgctggaacg aagtgtatta cggatttcgt cgctaagtag ccacatcagg tatgtaacgc
    11761 ctgatgcgac gctgactcgt cttatctggc ctacacgctg cgattttgta ggccggataa
    11821 gcaaagcgca tccggcattc aacgcctgat gcgacgctgg cgcgtcttat caggcctacg
    11881 cgctgcgatt ttgtagaccg gataagcgaa gcgcatccgg cacgaaggag tcaacatgtt
    11941 agaagatctc aaacgccagg tattagaagc caacctggcg ctgccaaaac acaacctggt
    12001 cacgctcaca tggggcaacg tcagcgccgt tgatcgcgag cgcggcgtct ttgtgatcaa
    12061 accttccggc gtcgattaca gcgtcatgac cgctgacgat atggtcgtgg ttagcatcga
    12121 aaccggtgaa gtggttgaag gtacgaaaaa gccctcctcc gacacgccaa ctcaccggct
    12181 gctctatcag gcattcccct ccattggcgg cattgtgcat acgcactcgc gccacgccac
    12241 catctgggcg caggcgggtc agtcgattcc agcaaccggc accacccacg ccgactattt
    12301 ctacggcacc attccctgca cccgcaaaat gaccgacgca gaaatcaacg gcgaatatga
    12361 gtgggaaacc ggtaacgtca tcgtagaaac ctttgaaaaa cagggtatcg atgcagcgca
    12421 aatgcccggc gttctggtcc attcccacgg cccgtttgca tggggcaaaa atgccgaaga
    12481 tgcggtgcat aacgccatcg tgctggaaga ggtcgcttat atggggatat tctgccgtca
    12541 gttagcgccg cagttaccgg atatgcagca aacgctgctg gataaacact atctgcgtaa
    12601 gcatggcgcg aaggcatatt acgggcagta atgactgtat aaaaccacag ccaatcaaac
    12661 gaaaccaggc tatactcaag cctggttttt tgatggattt tcagcgtggc gcaggcaggt
    12721 tttatcttaa cccgacactg gcgggacacc ccgcaaggga cagaagtctc cttctggctg
    12781 gcgacggaca acgggccgtt gcaggttacg cttgcaccgc aagagtccgt ggcgtttatt
    12841 cacgccgatc aggttccccg cgctcagcat attttgcagg gtgaacaagg ctttcgtctg
    12901 acaccgctgg cgttaaagga ttttcaccgc cagccggtgt atggccttta ctgtcgcgcc
    12961 catcgccaat tgatgaatta cgaaaagcgc ctgcgtgaag gtggcgttac cgtctacgag
    13021 gccgatgtgc gcccgccaga acgctatctg atggagcggt ttatcacctc accggtgtgg
    13081 gtcgagggtg atatgcacaa tggcactatc gttaatgccc gtctgaaacc gcatcccgac
    13141 tatcgtccgc cgctcaagtg ggtttctata gatattgaaa ccacccgcca cggtgagctg
    13201 tactgcatcg gcctggaagg ctgcgggcag cgcatcgttt atatgctggg gccggagaat
    13261 ggcgacgcct cctcgcttga tttcgaactg gaatacgtcg ccagccgccc gctgctgctg
    13321 gaaaaactca acgcctggtt tgcaaaccac aatcccgatg tgatcatcgg ctggaacgtg
    13381 gtgcagttcg atctgcgaat gctgcaaaaa catgccgagc gttaccgtct tccgctgcgt
    13441 cttgggcgcg ataatagcga gctggagtgg cgcgagcacg gctttaaaaa cggcgtcttt
    13501 tttgcccagg ctaaaggtcg gctaattatc gacggtatcg aggcgctgaa atccgcgttc
    13561 tggaatttct cttcattctc gctggaaact gtcgctcagg agctattagg cgaaggaaaa
    13621 tctatcgata acccgtgggt tcgaatggac gaaattgacc gccgtttcgc cgaagataaa
    13681 cctgcgctgg caacttataa cctgaaagat tgcgagctgg tgacgcagat cttccacaaa
    13741 actgaaatca tgccattttt actcgaacga gcaacggtga acggcctgcc ggtggatcgg
    13801 cacggcggtt cggtggcagc gtttggtcat ctctattttc cgcgaatgca tcgcgctggt
    13861 tatgtcgcgc ctaatctcgg cgaagtgccg ccgcacgcca gccctggcgg ctacgtgatg
    13921 gattcacggc cagggcttta tgattcagtg ctggtgctgg actataaaag cctgtacccg
    13981 tcgatcatcc gcacctttct gattgatccc gtcgggctgg ttgaaggcat ggcgcaacct
    14041 gatccagagc acagtaccga aggttttctc gatgcctggt tctcacggga gaaacattgc
    14101 ctgccggaga ttgtgactaa catctggcac gggcgcgatg aagccaaacg tcagggtaat
    14161 aagccgcttt cgcaggcgct gaagatcatt atgaatgcct tttatggcgt gctcggcacc
    14221 accgcctgcc gcttcttcga tccgcgcctg gcatcgtcga tcaccatgcg tggtcatcag
    14281 atcatgcggc agaccaaagc attgattgaa gcgcagggct acgacgttat ctacggagat
    14341 accgactcga cttttgtctg gctaaaaggt gcacattcgg aagaagaggc ggcgaaaatc
    14401 ggtcgtgcac tggtgcagca cgttaacgcc tggtgggcgg aaacgctgca aaaacaacgg
    14461 ctgaccagcg cattagaact ggagtatgaa actcatttct gccgttttct tatgccaacc
    14521 attcgcggag ctgataccgg cagtaaaaag cgctacgccg ggctgattca ggagggcgac
    14581 aagcagcgga tggtgtttaa agggctggaa accgtgcgca ccgactggac gccgctggcc
    14641 cagcagtttc agcaggagct gtacctgcgt atcttccgca acgagccata tcaggagtac
    14701 gtccgccaaa ccatcgacaa actgatggcg ggtgaactgg atgcgcggct ggtttaccgt
    14761 aaacgccttc gccgtccgct gagcgaatat cagcgtaatg ttccgcctca tgtacgcgcc
    14821 gctcgccttg ccgatgaaga aaatcaaaag cgcggtcgcc ccttgcaata tcagaatcgc
    14881 ggcaccatta agtacgtatg gaccaccaac ggcccggagc cgctggacta ccaacgttca
    14941 ccactggatt acgaacacta tctgacccgc cagctacaac ccgtggcgga gggaatactc
    15001 ccttttattg aggataattt tgctacactt atgaccgggc aacttgggct attttgagca
    15061 aaaaaagagt tcgccagata ccattttgat gcgtgacgaa tgctttgcca tccagtacca
    15121 tagcgccctt tccattcctg gacctgaata acaccactac ctcataagca cggtagcggg
    15181 tggttattgc ctgcaattaa agatatagag ccgaacacat atgcctttta cacttggtca
    15241 acgctggatc agcgatacag aaagcgaatt gggacttgga accgttgtcg cggtggatgc
    15301 gcgaactgtc actttacttt tcccatctac tggtgaaaac cgtctgtacg cacgcagtga
    15361 ttcccccgtg acccgcgtga tgttcaaccc tggtgatacc attaccagcc atgacggctg
    15421 gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg ctgacctata tcggtactcg
    15481 cctggatact gaagagtccg gcgtagccct gcgtgaagtt ttccttgata gcaaactggt
    15541 gttcagcaaa ccgcaggacc gtctgtttgc cgggcagatt gaccgtatgg accgctttgc
    15601 gctgcgttat cgcgcgcgta agtattccag cgaacagttc cgtatgccgt acagcggcct
    15661 gcgcggtcag cgtaccagcc tgatcccgca tcagctcaac atcgctcatg atgttggtcg
    15721 ccgccacgcg ccgcgcgtcc tgctggctga cgaagtgggt ttagggaaaa ccattgaagc
    15781 cgggatgatc ctgcatcagc aactgctctc tggcgctgct gaacgtgtgc tgattatcgt
    15841 cccggaaacc ttacagcatc agtggctggt agaaatgctg cgccgtttca acctgcgctt
    15901 tgctctgttt gatgatgagc gttatgccga agctcagcac gatgcttaca acccgtttga
    15961 caccgaacag ctggtgattt gctcgctgga ttttgcccgt cgtagcaaac agcgcctgga
    16021 acatctctgt gaagccgaat gggacctgct ggtggtcgat gaagcgcatc acctggtgtg
    16081 gagcgaagat gcgccaagcc gtgaatatca ggccattgaa caactggcag agcacgtgcc
    16141 gggcgttctg ctgctgaccg cgaccccgga acagctgggg atggaaagcc acttcgcccg
    16201 tctgcgtctg ctggacccga accgtttcca cgattttgcg cagttcgttg aagagcagaa
    16261 aaattatcgt ccggttgcgg acgccgttgc cattctgctg gcaggtaaca aactgagcaa
    16321 tgacgaactg aacatgctcg gcgagatgat cggcgagcag gatatcgagc cgctgttgca
    16381 ggcagcaaac agcgacagcg aagatgccca gagcgcccgt caggagctgg tttcgatgct
    16441 gatggatcgc cacggcacca gccgcgtgct gttccgtaac acgcgtaacg gtgtgaaagg
    16501 attcccgaaa cgcgagctgc acaccattaa gctgccgcta ccgacgcagt atcagacggc
    16561 tattaaagtc tccggcatta tgggcgcacg taaaagtgcg gaagatcgtg ctcgcgatat
    16621 gctctacccg gagcgtattt atcaggaatt tgaaggtgat aacgccacct ggtggaactt
    16681 cgatccgcgc gttgagtggc tgatgggcta cctgaccagc catcgctctc agaaagtgct
    16741 ggtgatctgc gccaaagctg ccactgcgct gcaactggag caggtactgc gcgaacgtga
    16801 aggtattcgc gctgcggtgt tccacgaagg tatgtcgatt atcgaacgtg accgcgctgc
    16861 cgcctggttt gccgaagaag acaccggcgc acaggtactg ctgtgctcag aaatcggttc
    16921 tgaaggacgt aactttcagt tcgccagcca catggtgatg tttgacctgc cattcaaccc
    16981 ggatctactg gagcagcgta ttggtcgtct ggatcgtatc ggccaggcgc acgatattca
    17041 gatccatgtg ccttatctgg agaaaaccgc tcagtcggtg ctggtgcgct ggtatcacga
    17101 aggtctggat gcatttgagc acacctgccc gaccggacgc actatttacg atagcgtata
    17161 caacgatctg attaactatc tggcttcacc ggatcaaacc gaaggctttg acgatctgat
    17221 caaaagctgc cgcgagcaac atgaagcgct gaaagcacag ctggaacagg gtcgtgaccg
    17281 cctgctggaa atccactcca acggtggcga aaaagcccag gcactggcag aaagcattga
    17341 agagcaggat gacgatacca acctgatcgc cttcgccatg aacctgttcg atattatcgg
    17401 tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg acgccgtccg atcatatgct
    17461 ggtgccggac ttccctggcc tgtcggaaga tggcatcacc atcacctttg atcgtgaagt
    17521 ggcgctggcg cgtgaagatg cacagtttat tacctgggag catccgctga tccgcaacgg
    17581 tctggatctg atcctttctg gcgataccgg tagcagcacg atttcactgt taaaaaacaa
    17641 agcgttgccg gtaggtacgc tgttggtgga actgatttat gtggttgaag cccaggctcc
    17701 gaagcagttg cagctcaacc gcttcctgcc accgacgccg gtacgtatgc tgctggataa
    17761 aaacggcaac aacctggcgg cgcaggtaga gtttgaaacc tttaaccgcc agcttaacgc
    17821 ggttaaccgt cacaccggca gcaaactggt taacaccgtg cagcaggatg ttcacgctat
    17881 ccttcaactg ggtgaagcgc agatcgagaa atctgcccgt gcattgattg atgcagcgcg
    17941 taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt ctggaagctc tgcgtgcagt
    18001 gaacccgaac attcgtgacg acgaactgac cgccattgag agcaaccgtc agcaggtaat
    18061 ggaaagcctg gatcaggcag gttggcgtct ggatgccctg cgtttgatcg ttgtaacgca
    18121 tcagtaacgg agccgaaaat ggggatggaa aactacaatc caccgcagga accctggttg
    18181 gttatcctgt atcaggatga ccatattatg gtggtcaaca agccgagcgg tttgttgtca
    18241 gtgccgggtc gtctggaaga gcacaaagac agcgtgatga cgcgcattca gcgtgattat
    18301 ccgcaggcag aatcggtgca tcgtctggat atggctacca gcggcgtgat tgtagtggcg
    18361 ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc gcgagcgcga gccgaaaaag
    18421 cagtatgtgg cccgcgtctg ggggcatcca tcccccgcag aaggtctggt ggatctgccg
    18481 ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct gttacgaaac gggtaaacct
    18541 gcgcagacgg aatatgaagt ggtggagtat gcggcggata acacggcaag agcggtgtta
    18601 aaaccgatta ccgggcgttc gcatcaattg cgtgtgcata tgctggcgct ggggcatccg
    18661 attctcggcg atcgttttta tgcatcacca gaagcgagag cgatggcacc gcgtttgttg
    18721 ctacatgcag agatgttgac gattacccat ccggcgtatg gcaatagtat gacgtttaaa
    18781 gcgccagcgg atttttaatc gtcacgctgg cgcagttcgg taggcggata aggcgttcac
    18841 gccgcatccg gcaattgtgc gccgattgcc tgatgcgacg cgggccgcgt cttatcaggc
    18901 ctacagttta aaacttattt aaaccctttc tgctgcttta tcagctcata tgcctgctga
    18961 atttcctgcg ctttctgctt cgccatctcc atcatctcag gcggcaaacc tttcgccacc
    19021 agcttatcgg gatggtgttc actcatcagc ttacggtagg cacgtttgat ggtggtcgca
    19081 tcatccgtcg gcttcacgcc cagcacatta caggcatctt ccagcgtcgg gccacgctgc
    19141 gcttgctgcc agttaccacc gccagattgc tgctgataac cgccgccaaa ctgtgcaccg
    19201 ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg agatccctaa ttcttcagca
    19261 atgacataca gcaccgcccg ttcattcggg tgcagtgaac catcagcaaa cgccgcctga
    19321 atctggatct ccagaaacat acgaattaag tcaaaacgac caaagcagac actgcgaaac
    19381 tggcgcatct tttcgcgcag cgggtaatta tctgattttc ccacccggaa cgcgttttgc
    19441 gccgcagtac gggaagcgcc atgaaggttc attcggtcca tcaactggct ggcgatatga
    19501 atatcagcct ccgtgacgcg acctttggat ttggttaaat gccccatcac ttcaaaagtg
    19561 gtggcaaaaa acagcgcctg acgctcacgc tggttggcga accacgccat tttacggcta
    19621 cgggctttat caaacatatg gccaattaac aggcctaaca ctacgcccca aaagccgccg
    19681 cccatcagta aggccacggc cacgccaatg atttttcccc aatactgcat atattcccca
    19741 aatcgacaca cggatatcag ggctatctcc cacaatataa aggtgctttt accgttttcc
    19801 ggctgcggtc aattgtggga catcgcctat aatttgcatt atcatacctg tcattcaatg
    19861 ccgtgcctaa caccactgac gctattcgga caggattaac actagcgtag agatgacaag
    19921 tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg atgatggaac aataaaatca
    19981 acgtatgaaa aaacgtatcc ccactctcct ggccaccatg attgccaccg ccctttatag
    20041 tcaacaggga ctggcagccg acctcgcctc acagtgcatg ttgggcgtgc caagctatga
    20101 ccgtcctctg gtacagggcg ataccaatga cttacccgtg actatcaatg ctgaccacgc
    20161 gaaaggggac tacccggatg acgccgtgtt tactggcagc gtggatatca tgcagggtaa
    20221 cagccgtctg caggccgacg aagtgcagct ccatcaaaaa gaggcaccag gacaaccgga
    20281 gccggtacgt accgttgatg cgctcggtaa tgtccattac gacgataacc aggtgatcct
    20341 caaagggccg aaaggctggg cgaatctgaa caccaaagat accaacgtct gggaaggtga
    20401 ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac ctgatgaaac aacgtggcga
    20461 aaaccgctat accattctgg ataacggtag ctttacctcc tgtctgccgg gttctgacac
    20521 ctggagcgtg gtaggtagcg aaattattca tgaccgcgaa gaacaagttg cggagatctg
    20581 gaacgcccgc tttaaggtgg gtccggtacc gatcttttat agcccctatt tgcagttgcc
    20641 ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac gccaagtaca ccaccaccaa
    20701 ctactttgag ttctacctgc catattactg gaacatcgcg ccaaatatgg atgccaccat
    20761 cacgccgcat tatatgcatc gtcgtggcaa catcatgtgg gagaacgaat tccgctacct
    20821 ctcccaggcg ggcgctggct tgatggaact ggactatctg ccttcagata aagtctatga
    20881 agatgaacac ccgaacgatg acagttcacg tcgttggtta ttctactgga accactccgg
    20941 ggtcatggat caggtgtggc gtttcaacgt cgactacacc aaggtcagcg atcctagcta
    21001 cttcaatgat ttcgataaca agtacggttc cagtactgac ggctacgcaa cgcaaaaatt
    21061 cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt tcaaccaagc agttccaggt
    21121 tttcagcgaa cagaacacca gtagctactc ggcagagccg cagttagacg ttaattacta
    21181 ccagaatgat gttggtccgt ttgatacgcg tatttacggc caggcagtgc actttgttaa
    21241 caccagagac gacatgcctg aagcaacccg tgttcacctg gaaccgacca tcaatttgcc
    21301 gctctctaat aactggggca gcatcaatac cgaagcgaag tttctggcaa cccattatca
    21361 gcaaaccaat cttgactggt ataactccag aaacacgacc aagctggacg aatccgttaa
    21421 ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc tttgaacgcg atatggaaat
    21481 gctggctccg ggttataccc aaacgctgga accgcgcgcg cagtatttgt acgtgccgta
    21541 tcgcgatcag agcgacatct ataactacga ctcgtctctg ctgcaatctg actactctgg
    21601 cctgttccgg gaccggactt acggcggtct tgaccgtatt gcctccgcta accaggtgac
    21661 gaccggtgtc acatctcgca tatatgatga tgctgccgtt gaacgtttta atatttccgt
    21721 tggtcaaatc tactatttca cggagtctcg cactggcgat gacaacataa catgggagaa
    21781 tgacgacaaa acgggctcac tggtgtgggc aggcgacacc tactggcgta tctccgagcg
    21841 ttggggattg cgtggcggga ttcagtacga tacacgtctg gataacgtag cgaccagtaa
    21901 ctccagcatt gaataccgtc gggatgaaga ccgtctggta cagctgaatt accattacgc
    21961 cagcccggaa tatattcagg ctacgctgcc taagtactat tccactgctg agcaatataa
    22021 gaatggtatt tcgcaggtag gtgctgtcgc cagcaggcca attgccgatc gttggtccat
    22081 tgttggggcc tactactacg acaccaatgc gaacaagcaa gccgactcta tgttaggtgt
    22141 gcaatacagc tcctgctgct atgcaattcg cgtcggttac gagcggaagc tgaacggttg
    22201 ggataacgat aaacaacatg cggtatatga caacgcaatc ggctttaaca tcgaacttcg
    22261 cggcctgagc tccaactacg gtctgggtac gcaagagatg ctgcgttcga acattctgcc
    22321 gtatcaaaac actttgtgat ttgttgattt accacgtaat ccgcagtgcg gttaattgaa
    22381 atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg tatcgccatg atcgcgaata
    22441 ccagtttcgc tgccccccag gtagtcgata aagtcgcagc cgtcgtcaat aacggcgtcg
    22501 tgctggaaag cgacgttgat ggattaatgc agtcggtaaa actgaacgct gcgcaggcaa
    22561 ggcagcagct tcctgatgac gcgacgctgc gccaccaaat catggaacgt ttgatcatgg
    22621 atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa aatctccgat gagcagctgg
    22681 atcaggcgat tgctaacatc gcgaaacaga acaacatgac gctggatcag atgcgcagcc
    22741 gtctggctta cgatggtctg aactacaaca cttatcgtaa ccagatccgc aaagagatga
    22801 ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat caccatcctg ccgcaggaag
    22861 tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc cagcactgag ctgaacctga
    22921 gccacatcct gatcccgctg ccggaaaacc cgacctctga tcaggtgaac gaagcggaaa
    22981 gccaggcgcg cgccattgtc gatcaggcgc gtaacggcgc tgatttcggt aagctggcga
    23041 ttgctcattc tgccgaccag caggcgctga acggcggcca gatggggtgg ggccgtattc
    23101 aggagttgcc ggggatcttc gcccaggcat taagcaccgc gaagaaaggc gacattgttg
    23161 gcccgattcg ttccggcgtt ggcttccata ttctgaaagt taacgacctg cgcggcgaaa
    23221 gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat tctgctgaaa ccgtcgccga
    23281 tcatgactga cgaacaggcc cgcgtgaaac tggagcaaat tgctgctgat atcaagagtg
    23341 gtaaaacgac ttttgctgcc gcagcgaaag agttctcgca ggatccaggt tctgctaacc
    23401 agggcggtga tctcggctgg gctacaccag atattttcga tccagccttc cgtgacgccc
    23461 tgacccgcct gaacaaaggt caaatgagtg caccggttca ctcttcattc ggctggcatt
    23521 taatcgaact gctggatacc cgtaatgtcg ataaaaccga cgctgcgcag aaagatcgtg
    23581 cataccgcat gctgatgaac cgtaagttct cggaagaagc agcaagctgg atgcaggaac
    23641 aacgtgccaa cgcctacgtt aaaatcctga gcaactaatg gttaaaaccc aacgtgttgt
    23701 gatcactccc ggcgagcccg ccgggattgg cccggactta gttgtccagc ttgcacagcg
    23761 tgagtggccg gtcgaactgg ttgtttgtgc cgatgccact ctccttaccg accgggcagc
    23821 gatgctcggt ttgccgctca ccctccgccc ttattccccc aactcccctg cacaaccgca
    23881 aactacgggc acattaacgc tacttcctgt cgcgctacgt gaatctgtca ctgcggggca
    23941 gttagcgatt gaaaatgggc attacgtggt ggagacgctg gcgcgagcgt gtgatggctg
    24001 tctgaacggt gaatttgctg cgctgatcac aggccccgtg cataaaggcg tcattaacga
    24061 cgcaagcatt ccgtttaccg gtcataccga gtttttcgaa gagcgttcgc aggcgaaaaa
    24121 agtggtgatg atgctggcga ccgaagaact tcgcgtggcg ctggcaacga cgcatttacc
    24181 gctgcgcgat atcgcagatg ctatcacccc tgcacttttg cacgaagtga ttgctatttt
    24241 gcatcacgat ttgcgtacaa aatttggtat tgccgaaccg cgcattctgg tctgcgggct
    24301 gaatccgcac gcgggcgaag gcggtcatat gggtacggaa gagatagaca ccattattcc
    24361 ggtgctcgac gagctgcggg cgcagggaat gaaactcaac gggccgctgc ctgccgatac
    24421 cctgtttcag ccgaaatatc tcgataacgc cgacgccgtg ctggcgatgt accacgatca
    24481 gggtcttccc gtgctaaaat accagggctt cgggcgcggt gtgaacatta cgctgggcct
    24541 gccctttatt cgcacatcag tggaccacgg caccgcgctt gaactggcgg gacgtggcga
    24601 agccgatgtc ggcagtttta ttacggcgct taatctcgcc atcaaaatga ttgttaacac
    24661 ccaatgaata atcgagtcca ccagggccac ttagcccgta aacgcttcgg gcaaaacttt
    24721 ctcaacgatc agttcgtgat cgacagcatt gtttctgcca ttaacccgca aaagggccag
    24781 gcaatggtcg aaatcggccc cggtcttgcg gcattgaccg agccggtcgg cgaacgtctg
    24841 gaccagctga cggtcatcga acttgaccgc gatctggcgg cacgtctgca aacgcatcca
    24901 ttcttaggcc cgaaactgac gatttatcag caggatgcga tgacctttaa ctttggcgaa
    24961 ctggccgaga aaatgggtca gccgctgcgt gttttcggca acctgcctta taacatctcc
    25021 acgccgttga tgttccatct gtttagctat actgatgcca ttgccgacat gcactttatg
    25081 ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga acagcaaagc gtatggtcga
    25141 ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg tactggaagt tccgccgtca
    25201 gcctttacac caccacccaa agtggattcc gccgtcgtgc gcctggtccc tcatgcaacg
    25261 atgcctcacc cggttaaaga tgttcgcgtg ttgagccgca tcaccaccga agcctttaac
    25321 cagcgtcgta aaaccattcg taacagcctc ggcaacctgt ttagcgtcga ggtgttaacc
    25381 ggaatgggga tcgacccggc gatgcgagcg gaaaatatct ctgtcgcgca atattgccag
    25441 atggcgaact atctggcgga gaacgcgcct ttgcaggaga gttaacgatg atcaattcgc
    25501 cccgagtgtg tattcaggtt caaagcgtct acattgaggc acaatcttca cctgataatg
    25561 aacgttacgt ttttgcttat accgtaacca tacgcaatct ggggcgagcg ccagtgcagt
    25621 tgttggggcg ttactggctg atcaccaatg gcaatggccg tgaaaccgaa gtccagggcg
    25681 aaggagtggt tggcgtccag ccacttatcg cgcctggcga agagtaccag tacaccagcg
    25741 gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca ctacgaaatg atcgatgaaa
    25801 atggcgtccc tttcagcatc gacattcccg tattccgact cgccgttccc acactcattc
    25861 attaaaagaa tatggcgaca taccttattg gcgacgttca tggttgttac gatgaactga
    25921 tcgcattgct gcataaagta gaatttaccc ccgggaaaga taccctctgg ctgacgggcg
    25981 atctggtcgc gcgcggcccg ggttcgctgg atgttctgcg ctatgtgaaa tccttaggcg
    26041 acagcgtacg tctggtgctg ggcaatcacg atctgcatct gctggcggta tttgccggga
    26101 tcagccgcaa taaaccgaaa gatcgcctga caccgctgct ggaagcgccg gatgccgacg
    26161 agctgcttaa ctggctgcgt cgccagcctc tgctgcaaat cgacgaagag aaaaagctgg
    26221 tgatggccca cgcagggatc acgccgcagt gggatctgca gaccgccaaa gagtgcgcgc
    26281 gcgatgtaga agcggtgctg tcgagtgact cctatccctt ctttcttgac gctatgtacg
    26341 gcgatatgcc aaataactgg tcaccggaat tgcgggggct gggaagactg cgttttatca
    26401 ccaacgcctt tacccgtatg cgtttttgct tcccgaacgg tcaactggat atgtacagca
    26461 aagaatcgcc ggaagaagcc cctgccccac tgaaaccgtg gtttgcgatt cctggccctg
    26521 tcgctgaaga atacagcatc gcctttggtc actgggcatc gctggaaggc aaaggtacgc
    26581 cggaaggtat ttacgcgctg gataccggct gctgctgggg tgggtcatta acctgcctgc
    26641 gctgggaaga taaacagtat tttgtccagc cgtcgaaccg gcataaggat ttgggcgaag
    26701 cggcggcgtc ttaaacacag cctgatacag gaaggccgga taagacgcga ccggcgtcgc
    26761 atccggcact agccgtaaat tctatacaaa cttaccgccg ctccagaatc tcaaagcaat
    26821 agctgtgaga gttctgcgca tcggcgtcgt ggaattcgct gaataccgat tcccagtcat
    26881 ccggctcgta atccgggaaa tgggtgtcgc cttccacttc tgcgtcgata tgcgtcagat
    26941 acagtttttg cgcttttggc aggaactgct cataaacgcg accgccgcca atcaccatga
    27001 tttctggtac gtcaccacac gccgcaatgg cttcatccac cgatttcacc cacgttacgc
    27061 gatcgtccgt acccggttga ctgctgagga taatattttt gcgtcctggc aacggacgac
    27121 cgattgattc ccaagtatgg cggcccataa tcacgggttt atttaaggtg ttgcgtttaa
    27181 accaggcgag atcggcaggc aggttccacg gcatggcgtt ttccatgccg ataacgcgat
    27241 ctaccgctaa cgccgcaatc agactgatca ttgagatttc ccgataaaaa aattgtcgcc
    27301 actatacgta aagcgtaaac cgtcgtcgac tggtgcgagg atgatgttga ggaaaatttt
    27361 atattctgct ggcgagtcca cgctctcccc caggactcgc cgcattacaa tgaaacaaaa
    27421 acaaacagtt agctgtaaag tgtgatttac gtcactcttt attaagatga gggtttcgtt
    27481 tccggttcat ccgccatgtt gccggtatgt ttaccttctt ctgttccctg ccagccatgt
    27541 cgttgaatta atgacagatg ttcgcggtcc tcggtgatga tctcacttaa catcgcgctg
    27601 gtgcgtttat agaccgccgc gcgggctttg gtgtcattct caaccattgc catctcttcc
    27661 accatctgaa tattaaagcg gcggaacaca tcggcacgtt ctcgcgcttc atacggcccc
    27721 agacctaaac tttccagcgc cagacgcccg gttttcagcg caccttcaaa ggtttcacgc
    27781 tccggctttt caacgcctgc ctgacgcaaa cgaatgtagt gatcgacatc gcgggcgcgg
    27841 gcaataatct gcaaatgcgg gaaatgttct ttcaccatct ccgtcagttg caggttggtt
    27901 tgcggatcgt caatagcgtt aatcagcact tccgctttcg ccgctccggc agattccagt
    27961 aaatccatcc gcgtggcatc gccataaaac actttcatac caaatttacg cagcgtttcg
    28021 atatggtccg gatcgtgatc gagcaccacc attttcaccc cgctggagag cagtaaacgt
    28081 ccggtaatct gtccaaaacg accgaatccg gcgataatca cgcgcggctg ttcttcatcg
    28141 atctcatcgg cttcacgcgc ctcctccgta gaagattgct caaggcgatt gaggatcacc
    28201 agcagaatcg gcgttgctgc catcgacagc gccaccgcca gggtcagcga tttcgcccac
    28261 tccggctcca gcacattcgc catctgcgcc gcaccaaata ccacaaaggc aaactcactg
    28321 ccctgcccta acaacaccgc aaaccagcga cgctgtttat ttggcacctg caacggtcgg
    28381 gcaatcagcc acagcatggc gattttgatg atgaggaaac cgagcagcaa aatgacaatg
    28441 cgcaatgggt tttcaatcag cgtgccaaag tctatcgaca tgccaacacc gatgaaaaac
    28501 agccccaaca gcaaaccttt aaacggttcg atatcgcttt ccaacgcatg acggtattcc
    28561 gagcttgcca gcagtacgcc cgccagaaac gcgcccatcg ccatcgacaa gccgacctct
    28621 tccagcagca aaccaaagcc aaacacaagg aataacgcca cggcactaaa cacttcccgc
    28681 aagccagagc gggcaacaaa acgcagcgcc ggacgcgtaa catagcgccc cagcaatacc
    28741 accagtacca gcgcacccgc cacttttaac gccgagagag caaatgcgcc catcgtcgtc
    28801 gaggcactgc tcgccgccag tagcgggatc atcgccacca gcgggattgc cgcgatatcc
    28861 tggaacagca gcactgcaaa ggcactgcgg cccatttgcg tcaccatcag attgcgttca
    28921 ttcatcgcct gcatggcaat cgccgttgat gagagcgcca gcgtcatacc gatcaattcc
    28981 gcgacctgcc agcgcagccc aagtaacata cagaacagcc ccagcaggcc accgcaaatc
    29041 accatctgca atgcgccacc gccgaacact gccgcacgca gcttccacag cctttgtgga
    29101 tcgagttcga ggccgataat aaacagcatc agcaccaccc caatctcggc aaagtgcaga
    29161 atagattcgg catcggtcac cagtcgcagc ccccacgggc caataatgca gccggcgatc
    29221 aggtagccaa gtaccgatcc cagaccaaga cgtaccgcaa tgggtacaat cagcgccgcc
    29281 gatccgaggt agatcagcgc ctgaaccagc gtatgactat ccatgatggg cctcctgcca
    29341 ttccagcaga cgttgcttat agtggcgcgc ctgcccttcg agggtttcgt cgtcacaaat
    29401 aaaggtgcag tgcatggcaa acggtggcag ccagttcagc ccgcagtaga ttgccgtcgc
    29461 ctgtagcggc tgcgacagca catcaaagcc cggatgcgca ccaatttcaa aatggctttc
    29521 cccgccgccg gtcgtcaccg cccacagcaa atgtttgcca tgcagcgccg tgccgccgtg
    29581 accgtaagcc cagccgtgcg acaaaacttt atcgatccaa agtttaagaa gcggaggaat
    29641 gctgtaccac tgcatcgggt gctgccagac gatcagatcg gcgcgagaga gcgcctcctg
    29701 ctcggcggca atatcgatat tgaagtcagg atagagttga taaagagagc gaatttcgac
    29761 gccttccagc gtccttgcct gttcaagcat ccgtttattc gcatgggaat gatgcggata
    29821 cggatgcgca taaatgatga ggatcatagt tattagcccg cgtgtttacg cctttgtttt
    29881 cccacaaagt gtagtcatat attcacgcgg cttatagtca ataatattga ttcagaaaag
    29941 agagaaatct gaacaaataa tcccattctc tgcccgcgga tcgtcatatt gctggtctgc
    30001 gccagtgaca ttcctcgagt ctccggggca aacgctacgg aaatcaacaa gccaaacagc
    30061 gagatacccg cccccattag catcgtgtta ctgataccgt aattattgat aaatatcggc
    30121 agtgcccagg tcgaaacaat ggtgccaata cgacttaagg acataatcac gcccacggca
    30181 gaggcgcgga tatccgtcgg gaagagttca ttaggataga gccactgcaa attacccggc
    30241 ccgccagaga aaaaggcata caccgcaaag gccatcacta ccagccagat ccccatatcc
    30301 gggattagcc ccaaaaccgc cagcgccagc gtcatcatgg caaagctgcc aatcaacaat
    30361 ggacgccgtc cggcagtgtt tagccacagc atcggcggaa tacagccgag cataaagaac
    30421 aggctaatca ccacattccc tagtgccgcg tttttgccaa cccccaatcc caacgaacca
    30481 acgatttgcg ggccaaaggt gtaaatggcg aacattggga tcacctggca ggtccagatg
    30541 gcggcaacaa acagaacaaa aggaaaatgg cggcgattaa acagatcgcg aaaacgggtt
    30601 tgctgcggct gctcttcatc gaaagccacc ggttcgccaa acagttttat catcatctct
    30661 tcgcactctt ttactcgccc tttgcgtaat aaccagcggg gagattctgg cagttcgaat
    30721 cgaccaatca aaatcaacaa acaagggatc gccgcgctac ccagcatcca gcgccaacca
    30781 ccttccacat cataaagcca gtagccgacc agatcggcgc aggtcgcgcc gacataccac
    30841 atcgcggcaa taaagctgat ggaaaacgcc cgctgacggg tactggagaa ctcggtgatc
    30901 atcgaagtgg cgatgggata atctgcaccg atgacaatgc cgatgagtac ccgcatcacc
    30961 aacagttcga cgggggatga aacacacatc gtcgccaccg atatcacgcc gatggcgatg
    31021 atatcaatga ggaacatttt gcgccgtccg actttatcgg agatatagcc aaacagcgat
    31081 gtgccaacga acagcccggc gagcgttccc gcgcccagca agccaatcca gtcagcgtcc
    31141 agtttcagcg ccggagtcag ttgctccagc gccacgccaa tcattaccag tacataacca
    31201 tccagaaacg gaccaccgct tccccacagc aaaatgcggc ggtgaataga ggagaatttg
    31261 agatcgtcaa agtttctgga cggttgcatg ggttattcct gtttttttgc aatgagagtc
    31321 agtacggcgt tacgtatcaa accaacatca gccgtaacgg aactccacac caaaggtgcc
    31381 gcgcgggtat tcccactgtt ccagcgccga ccccagcccc agaatgcgac aggtgccgca
    31441 ctccagacat ccggcgtaat cgaagcgcac actgccgtca tcctgcttct tgtacagacc
    31501 tgcggggcac gctttcacca gcagctccag cacctgttta tcagcatcgg ccttcacaac
    31561 aatgtgcgga tgctcttcat cgacattgaa tttattgacg cccagtttga cgtccacatt
    31621 gacgggagaa gtcataaaac ggtcactcct ttcatgccat ccttgatcag attgatgaag
    31681 cccactttct tgccgtggcg gaggattttc ttgcgcatca gttccggcgc actgccgtca
    31741 atggtgaaca ggtcacgcgc cacgcccacc gccagttccg ggtagccgct aaacatgcgt
    31801 gggttatcaa ggaacgccgg tagtttctgg tacatgcgca tatcgcgcag cgggctgctc
    31861 tccagatgct gacgatattc cgcgagtttt tgcttactga aatcgtcgct tttcatcgct
    31921 gaaagcacgg tttttgctgc agcttccccg gcggcaatcg ccagatccat accgcgaatg
    31981 gtaaaaccga ggttcataca cattccggcg gcatcaccgg caatcaatac gccgtcaccg
    32041 accagctccg gcagcatgtt gatgcctgct tccggcacta cgtgagcgga atattccacc
    32101 agcttgccgc ccgcgatcag cggtgcaacg gccggatgct gtttgaaatc ttccagcatt
    32161 tgcggcaccg attttttcgc gtcatgcaga tgatgcagac cacaaaccag ccccagcgac
    32221 agggtatttt cattggtata aaggaagccg ccgcccatca ggccatcggt gggtgatccc
    32281 gcaaacagac aagccgcgcc ctgattaccc tgcaactgaa aacggtcttc gattaccgac
    32341 ttcggtaact cgatcagttc cttcacgcca accgccacat ccgtcggttt aacgcgtttt
    32401 gccatcccca acttttcggc aaggatggag ttcaccccat cagcaaggag caccgttttc
    32461 gcttcaatca catcgccatc ggcttctaca ccgacgactt tgccatcgcg ctgtacgagg
    32521 ttatcgacgc ggatcccggt aattaactgc gcgcccgctt cttcggcctg ctccatcaac
    32581 caggcatcaa atttactgcg caaaacggag taagaacgct gggatagcga ggtttcgtca
    32641 ccgttgcagt agtccatagt catcgctgac ttttccgtca taaacgcgag tttttcatgg
    32701 gtgatcaggc gttctacggg ggcggaatcg gcgaaacctg gaataatgtg ttccagactg
    32761 tgggcataga gacgcccgcc agtaacgttc ttggcacccg cggaattgcc acgctcgata
    32821 actaacactt gcgccccttc gcgggcgagc accagtgcgg caaccgaacc ggcaagccct
    32881 gcaccgacga tgatggcgtc aaagatatct tcggacataa gagctccctg tcagcggcag
    32941 agccgtaatg cagccctgcc agagtggatc aacgcgctaa agctgcggtc agtgccggaa
    33001 ggatcttcac ggcatcgcca acaatgccgt aatccgcgta ctggaagatc ggcgcatttt
    33061 tatctttatt gatggcgaaa atggtttgcg acgcgttagc gccaaccatg tgctggatct
    33121 gcccggagat ccccaccgcc aggtacagtt caggtttcag catcaggttg gagataccga
    33181 catagcgttc gtgctccatc catttttcgt tttctgccac cggacgagaa caggccaact
    33241 ccgcacctat cgccttgcaa agctgttctg ccagtgcaat gttctctttg ctgccaatgc
    33301 cgcgaccgac gctgaccacc agacgggctt tgtcgagatc gacgctgttg ctctggcgcg
    33361 cctgggttgc cgtgcgggta atcgccacag ccggagcctg ccactccacg gtgtgcgttt
    33421 cgccagtgcg tgacgcgtct ggctgagccg catcgaacgt gccgctgctg atggtcagta
    33481 ccgcatacgg cgtggcaatg cgttcttcgc caatcgccag accgccgtaa accatgtgtt
    33541 tcactgttgc tttaccgtcc tgtacgctga cggtgctggc atcgttagac accgccgctt
    33601 taaggcgata acccagtttt gccgccagta atttgccgcg acgggtgttt ggcaacagca
    33661 ccagaccgtc agcgccgtgc tggcgaatag tgtcagccat gacaccggcg taatcttcga
    33721 tcatccgatc gtccggtttg ccgtttaatt tccagacatg attagcgccg agctggattg
    33781 cctgtgcgcc gtcggcatca ttgaggacaa aggcattgat ttgattagct aaagcctgcg
    33841 caccgttcat cagttccggc agacgagagg gggtatcgct gaatacccag acttgagaaa
    33901 acgtgttcat agcatcccct gtaattaaat gactttgcga agattttcag caaatgcggc
    33961 gatctgttct tcgccgtcgc cttcaatcac gatgcgctga cgctcgcgct gtttcggcgc
    34021 ggcaacctgt tgttcagacc aggcgttcac ggcgttaaaa ccaatatccg ccgccgacca
    34081 tacctggact ggctttttcg ccgcgccgag aatggctttc atcgaaggaa tttgtgggga
    34141 gttgatatca gtggaaacag caacaaccgc aggcagcgga atgcttaagg tttcggtttc
    34201 atcttccagt tcgcgctcaa cggtgagggt atctgccgtc agggagataa ttttgctgac
    34261 gccgttaact gccggaatat tgaggatttc gcccaccagc agaccaacct gctgggcata
    34321 aaggtcggaa gaaccatcgc cacagaggat cagatcaaag cctgctttct gggcggctgc
    34381 agccagtgcg ctcgccgttt gttgcggcag tgcctgctcg aactggtcat caatcaccac
    34441 aatcagttca tccgggccgc gcgatagcac atctttacgc cctttggcgt tggtcagggc
    34501 tttaccgccc acacttaagg ctgtcacctg cgcctctgct gcctgttgct ttagctggca
    34561 agccgcttca atagcgttga gatcgtattg gcttattttg gcatcggctt tgctgaagtc
    34621 taatgaacca tcagcattat tgaccgcaat atcctgttca tcaggcacgc acttatagca
    34681 agtaataatc ttcattgcat ctccagaaat catgaaggaa acgtcgttca tatcggtagt
    34741 gttaattaca gatatcacgc cctttaattt ggaaataaaa ctttaatcac caatattgaa
    34801 aatgtcacac gcagataaat ccaactttca atattgttaa gttcctcacc aatattgaaa
    34861 acatggcgta gcaaaaagaa attttcaata ttgttttata gatcaccaat attgaaagtc
    34921 agatgctatt ttttacccac aaaagaacat aacaaactga ttaataacca aaattaacga
    34981 aaaacgtcct ctattcactc gaaacaaaaa tgtgacacca atcacagaat acagcttatt
    35041 gaatacccat tatgagttag ccattaacgc gtccacgagg ttaataataa ttatattaaa
    35101 tgttaacaaa aataaaacaa acgggaacac aaaataaata ttcgttttca cagtggaatt
    35161 aactcatgaa gaatgaaaag agaaaaacgg gaatagaacc gaaggttttc tttccgccat
    35221 taataatcgt cggcatactt tgttggctta cagtcagaga tctggatgcg gcgaatgtcg
    35281 ttattaatgc tgtattcagt tacgtcacca atgtatgggg atgggcattt gaatggtata
    35341 tggtggtgat gcttttcggt tggttctggc tggtgtttgg cccgtatgcc aaaaagcgtt
    35401 taggtaacga accgccagaa tttagcaccg ccagttggat ctttatgatg ttcgcctcct
    35461 gtacgtctgc tgccgtactg ttctggggat cgattgagat ctactactac atctccaccc
    35521 cgccgtttgg cttagaaccg aactcgacag gagcgaaaga gttggggctg gcttacagct
    35581 tgttccactg gggacctctg ccgtgggcca cttacagctt cctttcagtc gccttcgctt
    35641 acttcttctt tgtccgcaaa atggaagtga ttcgccccag ctcgacactg gtgccgctgg
    35701 taggtgaaaa acacgccaaa gggttgttcg gtaccatcgt cgacaacttc tatctcgtcg
    35761 ccttgatctt cgcgatgggt accagtctgg gccttgccac gccgctggtg accgagtgta
    35821 tgcaatggtt gtttgacatt ccgcataccc tgcaactgga cgctatcatc attacctgct
    35881 ggattatcct caacgccatt tgcgtcgctt gcggtctgca aaaaggggta cgtatcgcca
    35941 gtgacgtgcg tagttacctg agcttcctga tgctgggttg ggtgttcatc gtcagcggtg
    36001 ccagcttcat catgaactac ttcaccgatt cggtggggat gttgctgatg tatctgccgc
    36061 gcatgttgtt ctataccgat cccatcgcta aaggcggctt cccgcagggc tggaccgtgt
    36121 tctactgggc atggtgggtg atttacgcca tccagatgag tatcttcctc gcccgcatct
    36181 cccgtggtcg tactgtgcgt gaactgtgct tcggcatggt gatggggctg acagcatcca
    36241 cctggatcct gtggaccgtg ctcggtagta acactctgct gttgatggat aaaaacatca
    36301 tcaacattcc aaatctgatc gaacagtacg gtgtggcgcg cgccatcatc gaaacctggg
    36361 ccgctctgcc actcagcacc gccaccatgt ggggcttctt catcctctgc tttattgcca
    36421 ccgttacgct ggttaacgcc tgctcttata ccctggcgat gtctacttgc cgcgaagtac
    36481 gcgatggtga agaaccaccg ctgctggtgc gtatcggttg gtcaattctg gttggcatta
    36541 tcggtattgt tctgctggcg ctcggcggcc tgaaaccgat tcaaaccgcc attatcgccg
    36601 gaggatgccc gctgttcttc gtcaacatta tggtgacgct ctcctttatt aaagacgcga
    36661 aacagaactg gaaagattaa ttaaccccca aaatatcaag aggttgaaag atggatttta
    36721 atttaaatga tgagcaggaa ctgtttgtcg ccggtatccg cgaactgatg gccagcgaaa
    36781 actgggaggc ctattttgcc gagtgcgacc gtgacagcgt ctacccggaa cgttttgtca
    36841 aagcactggc ggatatgggt atcgacagtc tgctgatccc tgaagagcac ggtggtctgg
    36901 acgcggggtt tgttactctc gccgccgtgt ggatggagct gggacgtctg ggggcaccaa
    36961 cctatgtact gtaccagctg ccgggcgggt tcaatacctt cctgcgcgaa ggcacacaag
    37021 agcagatcga caagattatg gctttccgcg gcaccggtaa gcagatgtgg aactcagcga
    37081 ttaccgaacc gggcgcgggc tccgacgtgg gtagcctgaa aacgacttat acccgtagaa
    37141 atggtaagat ttatcttaat ggtagtaagt gttttattac cagtagcgcc tacaccccgt
    37201 acatcgtggt gatggcgcgc gacggggctt ctccggacaa acctgtctac accgaatggt
    37261 ttgttgatat gagcaaaccg ggcatcaaag tgaccaaact tgagaagctc ggtctgcgta
    37321 tggatagctg ctgtgaaatc acctttgacg atgtggaact ggacgagaaa gacatgttcg
    37381 gtcgggaagg taacggcttt aaccgcgtca aagaagagtt cgaccatgaa cgtttcctgg
    37441 tagccctgac caactacggt acggcgatgt gcgcctttga agatgcggcg cgctacgcca
    37501 accagcgcgt gcagtttggc gaggctattg gtcgtttcca gttgattcag gaaaaattcg
    37561 cccacatggc gatcaaatta aactccatga aaaacatgct gtatgaagca gcgtggaaag
    37621 cagacaacgg caccatcacc tctggcgatg cagcgatgtg caaatacttc tgcgccaatg
    37681 cggcatttga agtggtggat agcgcaatgc aggtgctggg cggtgtcggg attgcgggca
    37741 accaccgcat cagccgcttc tggcgtgacc tgcgtgtaga ccgcgtttcc ggaggatctg
    37801 acgaaatgca gatcctgacg ctgggtcgtg cggtgctgaa gcaataccgc taagtccgtg
    37861 cgctgcctga tgcgacgctg acgcgtctta tcaggcctac gggagcacga atgtaagccg
    37921 gataaggcgt ttacgccgca tccggcagtc atgcgctaca taaatttttc aggagctaat
    37981 aatggatcat ctacccatgc cgaaattcgg accgctggcc ggattgcgcg ttgtcttctc
    38041 cggtatcgaa atcgccggac cgtttgccgg acaaatgttc gcagaatggg gcgcggaagt
    38101 tatttggatc gagaacgtcg cctgggccga caccattcgc gttcaaccga actacccgca
    38161 gctctcccgc cgcaatttgc acgcgctgtc gttaaatatt ttcaaagatg aaggccgcga
    38221 agcgtttctg aaattaatgg aaaccaccga tatcttcatc gaagccagta aaggtccggc
    38281 ctttgcccgt cgtggcatta ccgatgaagt actgtggcag cacaacccga aactggttat
    38341 cgctcacctg tccggttttg gtcagtacgg caccgaggag tacaccaatc ttccggccta
    38401 taacaccatc gcccaggcct tcagtggtta cctgattcag aacggtgatg ttgaccagcc
    38461 aatgcctgcc ttcccgtata ccgccgatta cttttctggc ctgaccgcca ccacggcggc
    38521 gctggcagca ctgcataaag tgcgtgaaac cggtaaaggc gaaagtatcg acatcgccat
    38581 gtatgaagtg atgctgcgta tgggccagta cttcatgatg gattacttca acggcggcga
    38641 aatgtgcccg cgcatgagca aaggtaaaga tccctactac gccggttgcg gtctgtataa
    38701 atgtgccgac ggctacatcg tgatggaact ggtgggcatt acccaaattg aagagtgctt
    38761 taaagatatt ggcctcgcac atctactcgg tacgccggaa atcccggaag gcactcagct
    38821 tatccaccgt atcgaatgcc cttacggccc actggtagaa gaaaaactcg atgcctggct
    38881 ggcggcacat accatcgcag aagttaaaga acgctttgcc gaactgaata tcgcctgtgc
    38941 caaagtactg accgtaccgg aactggaaag caatccacag tatgttgccc gtgaatcaat
    39001 cactcagtgg caaacgatgg atggtcgcac ctgcaaaggg ccgaacatca tgccgaaatt
    39061 caaaaataac cccggacaaa tctggcgcgg aatgccctca catggcatgg acacggctgc
    39121 cattttgaaa aatatcggct acagcgaaaa cgacattcag gagttggtca gcaaaggtct
    39181 ggccaaagtt gaggactaaa cgcgtggcgt cagtcggctt gcctgactag cgcccgccca
    39241 ataacaaacg aacaaaaatg gatagaggtg caatggatat cattggcgga caacatctac
    39301 gtcaaatgtg ggacgatctg gcggacgttt acggtcataa aacggcgctg atttgtgaat
    39361 ccagcggcgg agtcgttaac cggtatagtt atcttgagtt aaatcaggag attaaccgca
    39421 cggcaaacct gttttatacg ctggggattc gcaaaggcaa caaggttgca ctacatctcg
    39481 acaactgccc ggaatttatc ttttgctggt tcgggctggc aaaaattggc gcgattatgg
    39541 tgccgattaa cgcccgcctg ttacgcgagg aaagcgagtg gatcttgcaa aatagccagg
    39601 cgtgcctgct ggtgaccagt gcgcaatttt atcctatgta tcaacagatt cagcaggaag
    39661 atgccactca attgcggcac atttgcctga cagatgtggc acttcccgct gatgatggag
    39721 tgagttcatt tactcaactg aaaaatcaac aacctgccac cttgtgctat gcaccgccgc
    39781 tatcgactga cgatacggcg gaaattctct tcacctccgg caccacctcc cgaccgaaag
    39841 gtgtggtgat tacccattac aacctgcgct tcgctggata ttactccgcc tggcagtgtg
    39901 cactgcgtga cgatgacgtc tacatgacgg taatgcctgc gtttcatatc gattgccagt
    39961 gtactgcggc gatggcggcg ttttctgccg gggccacctt tgtgctggtc gagaaataca
    40021 gcgcccgcgc cttctgggga caggcgcaga agtaccgcgc caccattacc gaatgtattc
    40081 cgatgatgat ccgtacgttg atggtgcagc cgccttcagc gaacgatcgg caacaccgcc
    40141 tgcgggaagt gatgttttat ctcaacttgt cggagcagga aaaagacgca ttttgtgaac
    40201 gcttcggcgt tcgcttgctg acgtcttatg ggatgacgga aaccattgtg ggcattatcg
    40261 gcgatcgccc tagcgataaa cgacgctggc cgtcgattgg tcgggcgggg ttttgctacg
    40321 aagcggagat ccgcgacgat cacaatcgcc cgctcccggc aggtgagatc ggtgaaatct
    40381 gtattaaagg cgtaccaggg aaaaccatct tcaaagagta ttttctcaac ccgaaagcca
    40441 ctgcgaaagt gctggaagcc gacggctggc tacataccgg cgataccgga taccgcgacg
    40501 aagaaggctt tttttatttc gtcgatcgcc gctgcaatat gatcaaacgc ggtggcgaga
    40561 atgtctcctg cgtggagctg gaaaatatca tcgccaccca tccgaaaatt caggacatcg
    40621 tggttgtggg tattaaagat tcgattcgcg atgaagccat caaagcattt gtggtgctga
    40681 atgaaggtga aacattgagc gaagaggaat tttttcgctt ctgcgaacaa aatatggcga
    40741 aatttaaagt gccctcttat ctggagatca gaaaagatct gccacgtaat tgctcgggga
    40801 aaataattag aaagaatctg aaataaatga aacgtcaggg aactaccctg ccagctaata
    40861 atcacgccct aaaacaatat gcattttttg cagggatgtt gtcgtcccta aaaaagcaaa
    40921 aatggagaaa aggaatgagt gaatcattac atctgacccg caatggatca attctggaaa
    40981 ttacccttga tcgcccaaaa gcgaatgcta ttgatgcaaa aaccagcttt gaaatgggcg
    41041 aagtatttct aaatttccgt gacgatccgc aattacgtgt cgccattatt accggtgccg
    41101 gagagaaatt cttttccgcg ggctgggatt taaaagcggc agcagaaggc gaagcaccgg
    41161 atgctgactt tggcccgggt ggttttgcgg gattaaccga aattttcaat ctcgacaaac
    41221 cagttatcgc agctgtgaac ggctacgcct ttggcggcgg tttcgaactg gcgctggcgg
    41281 cagattttat tgtttgtgcc gataacgcca gcttcgccct gccggaagcc aaactgggca
    41341 tcgttcctga cagcggcggt gtgctgcgtc tgccgaagat cctgccgcct accatcgtca
    41401 atgaaatggt gatgaccggc agacgaatgg gcgcagaaga ggcgctgcgt tgggggatag
    41461 tcaaccgcgt ggttagccag gcggaactga tggataacgc ccgcgaactg gctcagcagc
    41521 tggttaacag cgccccgctg gcgattgcgg cgctgaaaga gatctaccgc accaccagcg
    41581 aaatgccggt agaagaagcg tatcgctata ttcgcagcgg cgtgttgaaa cactatccat
    41641 cggttctgca ttcggaagat gccattgaag ggccgctggc gtttgccgag aagcgcgatc
    41701 cggtgtggaa aggacgttaa caaccgtgag ctattacgcc tttgaggggt taattccggt
    41761 ggttcacccg acggcgtttg tccatcccag tgccgtcttg attggcgatg tgattgtggg
    41821 agccggtgtc tacatcggcc cactcgcctc actgcgtggt gactacgggc ggttgatcgt
    41881 gcaagcggga gccaatattc aggatggctg cattatgcat ggctactgcg acactgacac
    41941 catcgttggg gaaaacggcc atatcgggca tggagcgatc ctgcatggtt gtgtgattgg
    42001 tcgcgatgca ttggtcggga tgaacagcgt aattatggat ggcgcggtca ttggcgaaga
    42061 gagcattgtt gccgcaatga gctttgtcaa agcaggcttt agtggcgaga aacgccagtt
    42121 gttgatgggt acgccagccc gcgctgtacg cagtgttagt gacgacgagt tacactggaa
    42181 acggttgaat accaaagagt atcaggatct tgtcgggcgc tgtcatgcat cgttacatga
    42241 aacgcagcca ctgaggcaaa tggaggaaaa tcgcccccgt ttgcagggga cgacggatgt
    42301 gacgcctaaa cggtgatctt gccggatgcg acctggttgc tggataagat gctacaacat
    42361 cgtatctggc aagcaggacg cccggtatca acgagataaa attaacgacg catactcttt
    42421 gacaacatca tctgccactt ctgctcgcgg ttaagttccg tgagcggaac ggcaggaata
    42481 cgcggtgtct ttcttacgcc gactaatttt tcccgactgt tattgcgcag gcgcgcgtaa
    42541 atttgctcat cgatatcgac cactttaacc agtcgctgac actggcatcc ccgcccttcc
    42601 agcttattag ggatcatctt aacttcgcag cttatttctg tgacttccga cagaatataa
    42661 gtcagggtat taaccgcctt gctgtgttca atatcgaaat gaatagagat ctctcttgct
    42721 atcacccacc gattttcagc catcatccat tcggcgatta acaagtagag tggtttttca
    42781 acatatcctt cacacatacg aactccagtt tcacaaatta aaagtctggc tatgactcgt
    42841 tttgaagcaa ttaaacaaga ccatattaaa attgtggata tatcgatagt atgcaacttc
    42901 accgtcgata agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa
    42961 gatttactta atgggcaaaa aaaaacggtc ctcatcagag aaccgtatta gttattcgat
    43021 caaattagcg gatgaaaaat atccgccacg acacgctatt atttgatctg tgcgtgcatt
    43081 tcctgcaccg aaattacttt ttcagtcgca tcggcattca gcgccatcgc ggtagcgaaa
    43141 ccaccgttca gggtggtgtc ataatgcact ttatattgca gcgcactgcg acggatcacg
    43201 cgggagtctt caatcgcacg acggcctgag gtggtgttga tgatgtaggt atattcgcca
    43261 ttcttgatac ggtcctgaat gtgcggacgg ccttcatgca ccttgtttac cagacgcgga
    43321 ttgatacccg cttcgcccag cacaatcgcc gtgccgtggg tcgcatccag ctcgaagccc
    43381 tgtttcagca gttttgccgc caggtccacc acgcgttctt tatcgccttc gcgcacggaa
    43441 agcagcgcac gaccgtgttt cttcatggtg gagttgctgc ccagctgcgc tttggcgaac
    43501 gcttcagcga aggtgcggcc cacgcccatg acttccccgg tagagcgcat ttctggccct
    43561 aacagcgggt caacgcctgg gaatttattg aacggcagca ccacttcttt caccgagtag
    43621 tacggcggaa taacttcttt ggttacgccc tgctcagcca gcgatttgcc agccatcaca
    43681 cgcgccgcca cttttgccag cggtacgccg gtggctttgg agacgaacgg aacagtacgc
    43741 gccgcacgcg ggtttacttc aatcaggtaa acttcgttgt ttttcaccgc aaactgcacg
    43801 ttcatcagac cgcgcacctg caattcgaag gccagtttct gcacctgctg gcgcatcaca
    43861 tcctgaattt cctgacttaa ggtgtacgct ggcagcgaac atgcggagtc accggagtgc
    43921 acgcccgcct gctcgatgtg ctccatgatg ccgccaatca gcaccatttc gccgtcgcag
    43981 atggcatcca cgtcaacttc tactgcgtca tcaaggaaat gatccagcaa cactggcgca
    44041 tcgttagaca cgctaaccgc cgtctggaag tagcgacgca ggtcagcttc gtcatagacg
    44101 atttccatcg cccgaccgcc gagaacgtaa gacggacgca ccaccagcgg gtagccaatc
    44161 tcttttgcct tctcaactgc catttcaata gcagtaacgg tggcgttcgc cggttgtttc
    44221 agtttcagac ggtcaaccgc atgctggaag cgttcacggt cttccgcacg gtcgatagca
    44281 tccgggctgg tgccgataac cggtacgcca gcggcttcca gtgcacgcgc cagtttcagc
    44341 ggggtttgac cgccgtactg aacgataacg cctttcggct tctcaatacg cacgatttcc
    44401 agcacatctt ccagagttac cggctcgaag tagaggcgat cggaagtgtc gtagtcggta
    44461 gagacggttt ccgggttaca gttaaccata atggtttcgt aaccgtcttc acgcagcgcc
    44521 agcgaggcgt gtacgcagca gtagtcgaat tcgataccct gaccgatacg gtttggaccg
    44581 ccgccaagca ccatgatttt ttcacggtcg gtagacggat tcgcttcgca ctcttcttca
    44641 taagtggagt acatgtaagc ggtgtcggtg gcaaactctg ccgcacaggt atccacgcgc
    44701 ttgtagaccg ggtgcaggtc atattggtca cgcagcttac ggatttccgc ttcgcgtacg
    44761 cccgccagtt ttgccaagcg cgcatcggca aagcctttgc gtttcagctg gcgcaggaat
    44821 tcagcgttca ggccagtgat gcccacttcc gccactttct cttccagacg caccagttct
    44881 tcaatctgta ccaggaacca gcggtcaatg ttagtcaggt tgaagacgcc gtccacagac
    44941 aggcccgcgc ggaaagcatc ggcgatgtac cagatacgct ctgcgcctgc gtctttcagt
    45001 tcgcgacgga ttttggttaa cgcttccggg tcatccaggc tcactttcgg gtcgaagcca
    45061 gttgcgccca cttccaggcc gcgcagcgct ttttgcaggg attcctgctg cgtgcgacca
    45121 atcgccatca cttcgccaac cgatttcatc tgagtggtca gacggtcgtt agcaccggcg
    45181 aatttttcga agttgaagcg aggaatttta gtaaccacat agtcgatgga cggctcgaag
    45241 gaggccggag tacgtccgcc agtgatgtcg ttcatcagtt cgtctagggt gtaacccacc
    45301 gccagtttcg ccgccacttt agcaatcggg aaaccggtcg ctttcgacgc cagcgccgaa
    45361 gaacgggaca cgcgtgggtt catttcgata acaatcaggc gaccgttttt cgggttcacc
    45421 gcaaactgga cattggaacc accggtttca acgccgattt cacgcagcac cgccatcgag
    45481 gcgttacgca tgatttgata ttctttgtca gtcagcgttt gggctggcgc gacagtgatg
    45541 gagtcaccgg tgtggatgcc catcgcatca aagttttcga tagagcagac gatgatgcag
    45601 ttgtcgtttt tatcacgcac cacttccatc tcgtactctt tccagccgat cagcgactca
    45661 tcaatcagca actctttggt cggagagaga tccagaccgc gggcgcaaat ttcttcaaac
    45721 tcttcacggt tataagcgat accgccgccg ctaccgccca tggtaaagga tgggcgaata
    45781 atgcacggga agcccacgtc agcggcaaca gccagcgctt cttccatcgt gtgtgcgata
    45841 ccggaacgcg cggtttccag accaattttc ttcatcgcta cgtcgaaacg acggcggtct
    45901 tctgctttat caatcgcatc ggcagtggca ccaatcatgg tgacaccgaa ctcttccaac
    45961 acgccctgac gttccagctc cagcgcgcag ttcagcgccg tctgaccgcc catggttggc
    46021 agcaccgcgt ccgggcgctc tttttcaata atcttgcgta ccacttccca gtgaatcggc
    46081 tcgatgtagg tcgcatcggc catttccggg tcggtcatga tggtcgccgg gttggagttc
    46141 accagaatga cgcggtaacc ctcttcgcgc agggctttac atgcttgcgc gccagagtag
    46201 tcaaactcac acgcctgacc gataacaatc gggcccgcac ccagaatcag gatacttttt
    46261 atatctgtac gttttggcat ggctctttta ctcctgatta cttagcggtt ttacggtact
    46321 gctcaattaa cttgataaag tggtcgaaca acggcgcggc gtcgtgtgga ccagggctgg
    46381 cttcagggtg accctggaag ctgaacgccg gtttatcgat acgatgaatg ccctgtaacg
    46441 taccgtcgaa cagggattta tgcgtgacac gcaggtttgc aggtaatgtt gcttcatcca
    46501 ccgcaaaacc gtggttctgg gcggtgatca tcaccacgtt tttctccaca tctttaaccg
    46561 gatggttgcc gccgtggtga ccaaatttca ttttgacagt cttcgcaccg ctcgccagcg
    46621 ccagcagctg atgaccgaga cagatgccga ataccggaat atcggtttcg aggaacttct
    46681 ggatggcggt aatggcgtaa tcgcacgggg ccgggtcgcc aggaccgttg gagaggaaga
    46741 tgccgtctgg attcattttc agcacatctt ccgcagaagt ttgcgccgga acgatggtca
    46801 ggcgacagcc tctatccacc agcatccgca ggatgttgcg cttggcacca aaatcataag
    46861 ccacgacgtg gaacggcagc tcgtcttctt ttttcgcttc tggcaggccg ccggtcaacg
    46921 tccagctccc ttgtgtccag ctataggctt ctgcggtggt cacttctttt gccagatcca
    46981 tgccgttcag acctgggaac gcgcgggctt tttctaacgc cagcgccgca tccgggttat
    47041 cgcctgcgat aatgcagcca ttctgtgcgc ctttctcgcg cagtaaacgc gtcagcttac
    47101 gggtatcgat atcggcaatc gccacgatgt tatggcgctt caggtaagaa gagaggtctt
    47161 cggtattacg gaagttgctg gcaatcagcg gcaggtcgcg aatcaccaga ccttgtgcat
    47221 gtacctgaga agattcttca tcggcgtcat tagtgccgac attgccaata tggggataag
    47281 taagagtaac gatttgacga gaataggaag gatcagtgag gatttcttga taaccggtca
    47341 ttgaagtatt gaaaacgact tccccaaccg ccgaacctgt tgcccctatg gcccgaccgt
    47401 gaaactgggt tccgtcttcc agaaccaata gcgctgactt aatcaaaaca cctccataga
    47461 atattcactc actttatttg catattaatt cacagtgatg acgtaaatca atgcaaatct
    47521 gcttaccgac gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa
    47581 cttaatggca ggatttttta tattattttg tggctcatgc atgttttaca ggcattaagc
    47641 gacaaaaaca tcatctaacc tgttctggaa aacgcttgcg cagcaagaag attgcatgaa
    47701 ataaaaacga gcaggaaaaa gtggaccaaa tggtcaaaat ttacattaaa acaacaaaaa
    47761 tagaagacga aacatgtaat ttttggcagt tatagaaaat ttaaaagcat aacaccaaaa
    47821 ataaaagggc taaaaattaa agccctttat aattaataaa ttaaatgtgt tacttttgca
    47881 tcataataaa cattttgtgg ttacaaatta ttgagatcaa gcacatctcg catatcaaaa
    47941 agaccacttt ccttaccact caaccacaaa gccgatctta ccgcaccgtt agcaaatgtc
    48001 atgcggctgg acgccttatg ggtgatctcc agacgctcgc caatatcggc aaacatcgcg
    48061 gtatgttcgc caacgatgtc acctgcacgc acggtggcaa aaccaatggt accaggcaca
    48121 cgttcaccgg tatggccttc acgactgtag accgcgcaat ctttcagatc tttatcaagg
    48181 gcgtgggcga tcgcctctcc cattgccagt gcggtgcctg acggcgcatc aactttatgt
    48241 ctatgatgtg cttcaataat ttcgatatcg gtgtagtcac ccatcacttt ggctgctttc
    48301 tccagcagct taagcatgac gttaacgcca acgctaaaat tggcggcaaa gacaatcgca
    48361 atatcggcgg cggcgtcacg aattgcctgt ttaccggctt cgtcaaaccc cgtagtgccg
    48421 atcaccatcc ctttgccatg ctgccgacaa aaagcgagat ggttcagcgt accttccgga
    48481 cgggtaaaat cgataaacac atcaaaatca tcttttatcg catcaagact gctttgcacg
    48541 gtaacgcctg ttttccctgc accagccagc tcaccggcgt cgctgcccag taaagaagat
    48601 ccttcgcgct ccagcgccgc gcccagctgc acgccttcta atgccagtgc cgcctgaatc
    48661 aactggcggc ccatacgccc cccggctccc gcgatggcaa cgcggatgtt tgcatcatgc
    48721 atagctattc tcttttgtta atttgcatag accgttttca gagtaaccag cgcatcgcta
    48781 cgccgccagc caaaaacgct gataattaga aattaatgat aaacagggag ggatatcagt
    48841 gaaaggcatg agtcgatgaa tgactgcatg ccgataacat gtgacaggtt acggcaccag
    48901 agccagcacc tcagccaccc actgttggaa gccttttaca ttcagatcca atgccacctg
    48961 tacattggct ggtttgccca ggcaaccgtc gatatcaacc accgtcgtgc ctgaggtaaa
    49021 ttcgccttga gtttctaccg cgacaaaaca cggcttgagg gtgaataagt ccgggcgcac
    49081 cagccaggcg atggcgcaga gatcgtgcat tcgcagaccg ctttgcatac tgccgctacg
    49141 gtagtggcta aacagggcgt ggagcatttt tccggtgcga ttcaactccg gtaaagtagc
    49201 gagatagtca ggggttaata ttgcctgatt ggtgacatcc agaccgcaca tgacaatttc
    49261 gatgccgcta cggaagacac aggcagccgc ttctggatcg gcagcgatat taaactcggc
    49321 atttggtgta cagttgccgc gaccagcaga accgcccatg atcaccagac gacgaatata
    49381 cggtttacat tccgggcatt gtaaaagtaa cagcgcaata ttggttaacg ggccgatggc
    49441 caccagggta acgggctctg gtgcacgcat cagggcatcc cgaatcgcca gaaacgccgg
    49501 tatcccgagt ggctttcggt tgtgctcaac aaagtcgtag ccagccattc ccgattcgcc
    49561 gtgcacagat gctgcatcac gcggtgcgcg taccagcggc acagcggctc cctgggcgag
    49621 cggaatctcc gcattccaga aatgcagcag ttgcagggca ttgcgggtag ttttctcaac
    49681 cgagacatta cccgcgacgg tggtcatcag ttgcaggtcg agttcggggg caaaaatcgc
    49741 ggcggcaatg gcgacggcat cgtcaatgcc ggggtcagta tcgaggaaga taggtaaacg
    49801 catgttttct tcataaaaaa tgccggtaac aagaccggca ttttcgcata acttaggctg
    49861 ctaatgactt aatcgacttc acgaatatcg acacgcagct ctttcggcac ttcgaaaaca
    49921 atattttctt cacggccttc cagcggaatg gcttcaccac cacccaactg ctgcaaacgt
    49981 gccaccacat tctgcaccag aatatccgga gccgatgcgc ccgcagtcac gccgacgcat
    50041 ttaacctctt tcacccactc ttcctggata tctttcgcat cgtcaatcaa aaacgcgtgt
    50101 ttgcccatac gctgggccag ctccgccaga cggttggagt tggaggagtt tttcgaaccg
    50161 accaccaaca caacttccgc ctgttctgcc agggcgcgta ccgcttcctg acggttagtc
    50221 gtggcgtagc agatgtcatc tttgcgcgga ccgacaattt tcgggaagcg tttacgcagc
    50281 gcgtcgatca catcagacgt atcatccacc gacagcgtgg tttgggtcat aaaggagagc
    50341 ttctcttcgt ttttgaccgt cagtttccac acatcgtctg gcgattcgac cagatacatt
    50401 cccccttcgg ggttgctgta ctgccccatc gtcccttcca cttccgggtg cccggcgtgg
    50461 ccgatgagaa tagattcttc gccacggcga ctggcgcggg caacttccat atgcactttg
    50521 gtcaccagcg gacaggtggc atcaaacacc gtcaaatcgc ggcttttcgc ttcgttacga
    50581 accgcctgag aaacaccgtg tgcggagaaa atcaggatcg cgccgtccgg cacttcgcta
    50641 atctgctcaa taaagatagc cccacgctcg cgcaagctat cgaccacgta gcggttatgc
    50701 accacttcgt gacggacata tatcggtgcg ccgtaaatgg ccagcgcgtt ttcaacaatg
    50761 ctgatagcgc ggtctacccc ggcacaaaaa ccacgcgggt tggccaacag gatctgcatg
    50821 ttacgcctcc agtgccggat cgatttccag cacttcaata tcaaaatgaa cggtctgccc
    50881 ggccagcgga tggttgaaat caacggtaat ggagtcgccg ttaatttcgc ggatcacacc
    50941 aggcatctca ctgccatcca ttgcggtaaa aagcatgatt gcgccaattt ctggctcgcc
    51001 tgcatccata aattcacggc gggagaagta ctgaatcagg tccggtgacg gcacgccaaa
    51061 cgccgcatct ggctccagcg agaaggtggt tttgtcgccc actttcagcc ccagcaggtg
    51121 ttgctccagc ccttcagaaa gagaagcatc gcccaggcgg aacagcgccg gtttaccgtt
    51181 gttgcgagta gactcggcgg tggtgccatc gtcgagtttt agcgtgaagt gcaccaggac
    51241 ggcgctattg ctctgtacag attcagacat gaaggtttgc tcttattttt gaggattttt
    51301 gccggatgcg gcctacgatt tcgtagcaat ctgtaggccg catccggcag ggtttattat
    51361 tgttttttcg ctttggaagg taaaaaccct tccagcacga tcagtgccgc accgacacaa
    51421 atggcagtat cggcaaggtt gaaggtggcg aagtgccagt cgccgacgta gaagtcgatc
    51481 atatcgacaa cgaagccgtg ccacaggcgg tcgaacaggt tgcccagcgc gccgccaata
    51541 atcagcgcgt aagcgatatt gtttagcttc tgcgtggcct tcgagcgata catcatcact
    51601 gccaggagca cgctaatacc aatcgcaata ccggcaaaga accaacgctg ccagccgccg
    51661 ctatcggcaa ggaaactaaa cgccgcgcca tagttacgcg cataatgcag attaagcgac
    51721 gggaacagcg ggaccgtatc ccccagagca aagttctgga ggatcaggta tttgctgccc
    51781 agatcgataa tcagcacgac taccaccagc cacagccagc gtagccctgt tgaacagatc
    51841 gattgactca tcaggcaaac ttacgttttt caccgtcacc ggcgacgttg ctgacacagc
    51901 ggccgcagat ttctgcgtgt tccgccacct tgccgacatc ctgggtgtag tgccagcagc
    51961 gtgggcactt ctcaccttcg gctttactca acgcgacttt cagccctttg agtacttcgc
    52021 tctgctgagc atcagcaggt gcgtcgttat agtctgcaac ggtagcgccg gaggtcaaca
    52081 ggacaaatcg taattcatcg cccagcgcgg tcagtttcgc cgccagttcc ggttctgcat
    52141 acaaggttac tgccgcttcc agcgagccac ccactttctt gtcggcacgc gcttgctcaa
    52201 tgactttgtt cacttcgcca cgcactttca acagctcgtc ccagaacgca tcgttcattg
    52261 cttcactgtc tgccagacca aacaggcctt cgtaccactc accggtgaag acgtattttt
    52321 cacgttcgcc cggcagatag ccccacactt catcagcggt gaaggagagg attggtgcca
    52381 tccagcgcac cagcgcttca gcgatgtgat acagcgcagt ctggcagcta cgacgcgcca
    52441 cgctatccgc tttggcggtg tactgacggt ctttgatgat gtcgaggtag aaggaaccca
    52501 tctcaacgga gcagaagcgc atcagacgct gtaccacttc gtggaaatcg tatgcttcgt
    52561 acgccttgag gatgtcttcc tgtgccgctt tcgcacaacc tacggcccag cgatccagta
    52621 ccaccatctc ttccggtttc accatatctt ttgctggatc aaaaccattc aggtttgcca
    52681 gcaggaagcg cgcggtgtta cggatacgac gatagctatc ggcagcacgt ttcaggatct
    52741 cgtcagaaac ggccatttca ccggtgtagt cggttgatgc cacccacaga cgcagaatat
    52801 ccgcgcccag tttgttcatc acatcctgcg gcgaaacggt gttgccgatg gatttagaca
    52861 tcttgcgacc ctgaccatcc acggtaaagc cgtgggtcag tacctgacga tacggcgctt
    52921 tgcctttcat cgcggtggag atcattaggg aagacatgaa ccaaccacgg tgctggtcag
    52981 aaccttccag atacatgtcc gctgcgtgac cggcaaattc cggacgcacg tcaacaacag
    53041 aagagtgggt agatccggag tcaaaccata cgtcgagagt atccggcact ttcacatact
    53101 ggtcagcttc gtcgccgagg atctctttcg catcgagatc ccaccacgcc tggatgccgt
    53161 caacttcaac gcgtttagcc acttcttcca tcagttcgag ggtacgcgga tgcagctctt
    53221 ccgtgtcttt gtgcacgaac agtgacatcg gtacgcccca ggtgcgctga cgggagatac
    53281 accagtcagg acggttagca accatcgact cgatacgcgc ctggccccag tccgggatcc
    53341 actgcacgcc tttgatctct ttcagtgact gcgcacgcag acctttctga tccatgctga
    53401 cgaaccactg cggtgtcgcg cggaagatga tcggcgtttt gtgacgccag cagcacggat
    53461 agctgtgctg cattttctca acgtgcagca gcgcgccttt ttcctgcagc agcgcaacga
    53521 cgatgtcgtt cgctttgaag acgttcacgc catccagcgt cggataagtg cccggcagat
    53581 aagtgccgtc cgggccaacc gggttagcgg tttccaggcc gtatttctga ccgatcacat
    53641 agtcgtccgg gccgtggcca ggcgcggtgt gaacggcacc ggtaccggca tccagggtaa
    53701 cgtgatcgcc gaggattgcc ggaacgtcga agcccataaa cggatgggta aagcgcagca
    53761 gctcaagctc tgcacctttt accgtgccga gaatggtgta atcggtcacg ccgatacgct
    53821 gcattacgct ttcaaccaga tctttcgcca gaatcgcggc ctgaccgtcg atctgcacca
    53881 gcgcatagtc gaaatcaggt gcaatagaga ttgcgcggtt cgcaggcaaa gtccacggcg
    53941 tagtggtcca gatcaccagc gagattgggc cgttaacgtt gcttacgcca aatttcgctt
    54001 tcagcgcatc ctgatcgacc gcctggaaag cgacgtcaat ggacggagaa gttttgtcgt
    54061 aatactcaac ttccgcttct gccagtgcag aacggcagtc tacgcaccag tgcaccggct
    54121 tcgcgccttt gtgcaggtga ccattgccga tgattttgcc cagcgcgcgg atgatgttgg
    54181 cttcagtttt gaagtccata gtcagatacg ggtgcgacca gtcgcccagc acgcccagac
    54241 ggataaagtc tttgcgttga ccgtcaacct gggtcgccgc gtattcgcgg cacttggcgc
    54301 ggaactcggc ggcggtgaat ttctcacccg gcttaccgta ttcttgctct actttcagct
    54361 cgatcggcag accgtggcag tcccagccag gcacatacgg cgagtcataa ccggaaagcc
    54421 ctttggactt cacgataatg tctttcagaa tcttgttaac cgagtgacca atatgaatgc
    54481 tgccattcgc ataaggaggg ccatcatgca gaatgaaggt ttttttgcct tttttagccg
    54541 cacggatgat gccgtacaga tcatcatcag tccaacgcgc cagcattccc ggttcgcgct
    54601 tggcgagatc gccacgcatc gggaaccctg tttccggcaa attcagggtt gatttatagt
    54661 cactcatcag attctcggtt ccgtgtttcg gtttgattac ataacaggct taagccggtt
    54721 ttgttagccc aaaaaattcg cgggcggtta attcatcacg cgcaatctgc gctttcagtt
    54781 cgtccagcga cgcaaatcgc tgctcattgc gtattttttt acgcagcact acttgtatat
    54841 ggcgaccgta aaggtccatt gcaacatcta acaaatgcac ttccagctgc tggcgaatac
    54901 cggcaaccgt tgggcgtgtt ccgatgtttg ccacgccggg taacggcttt tcaccgaggc
    54961 ccagcacttc taccgcataa acccctttca ccggggaaac ctgacggcgt agcggtacat
    55021 tcgccgtcgg gaaacctata gtgcgcccta attcatcacc gtggactaca cgcccggaga
    55081 tggcaaacgg gtgccccagt aaactttctg ccagagccag attgtcatca gccagcgcct
    55141 gacgcacggc ggtgctgctg atacgcacac caccttcgca aaaagtttgc gtactggtga
    55201 tatcgaagcc gtattccatg ccagctttct gtaataacaa gaaatcgcct tcacgaccag
    55261 cgccaaagcg gaaatcatca cctacggcaa gaaattttac gcgcaaatgc ttcaccaaaa
    55321 gatcgctgat gaaattttgc gcggttaacg ccgcaaaacg cctgtcgaaa cgcacgcaca
    55381 gcacgtaatc aacgccacac tctgcaaggt aacgcagttt ttcccgcagc cgggtcagtc
    55441 ttgccggggc tttatcggta gcaaacagtt ccagtggttg aggttcaaaa agcatcacca
    55501 tcaccggtaa gttgcgcttg cgcccttctt cctgcaagcc ctgtaacagc gcgcgatgac
    55561 cgcgatgcac gccgtcgaaa ttaccaatag tcagcacaca cccttcttgc ggggcctggc
    55621 tgagattatg tatgccgcgt atcagcttca tgtctggctc aaaacagtga aaatcgtccg
    55681 agtatacctt gtacagcggt caaggttaac cggcgattga gtaccgaaat cagaaagaag
    55741 tggcttttca ttgccatggc gcaaatcacg ggaagaaact gaccgcctgc tgcaattttt
    55801 atcgcggaaa agctgtattc acaccccgca agctggtaga atcctgcgcc atcactacgt
    55861 aacgagtgcc ggcacattaa cggcgcttat ttgcacaaat ccattgacaa aagaaggcta
    55921 aaagggcata ttcctcggcc tttgaattgt ccatatagaa cacatttggg agttggacct
    55981 tggctaatat caaatcagct aagaagcgcg ccattcagtc tgaaaaggct cgtaagcaca
    56041 acgcaagccg tcgctctatg atgcgtactt tcatcaagaa agtatacgca gctatcgaag
    56101 ctggcgacaa agctgctgca cagaaagcat ttaacgaaat gcaaccgatc gtggaccgtc
    56161 aggctgctaa aggtctgatc cacaaaaaca aagctgcacg tcataaggct aacctgactg
    56221 cacagatcaa caaactggct taatcgccaa tttgctgaag ctttgtgaaa aagcccgcgc
    56281 aagcgggttt ttttatgcct gctgcttttg tgatgcgtga gagaacacac tgccggttgc
    56341 cagatagtca aaagagacat gccgttgtac tatctgaact cttcgtggct taagaatcgt
    56401 agaaccatcg gctgatacat tctgaacaag aatatctact caatctccag actcccccac
    56461 aaagaatatg gatattgtga tacacattga ggtattaaac ttaagaccac tctatttttg
    56521 aattaaattc ctatatactt taaccattca taaatatctc ctcattagac
//
LOCUS       DAJTYF010000013        53214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTYF010000013 DAJTYF010000000
VERSION     DAJTYF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.013"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(15..326)
                     /locus_tag="OO590_000885"
     CDS             complement(15..326)
                     /locus_tag="OO590_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6636355.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            489..585
                     /gene="ffs"
                     /locus_tag="OO590_000886"
     ncRNA           489..585
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO590_000886"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            705..1058
                     /locus_tag="OO590_000887"
     CDS             705..1058
                     /locus_tag="OO590_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6636356.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1100..2650)
                     /gene="pdeB"
                     /locus_tag="OO590_000888"
     CDS             complement(1100..2650)
                     /gene="pdeB"
                     /locus_tag="OO590_000888"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR6636357.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLHQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2814..3284)
                     /locus_tag="OO590_000889"
     CDS             complement(2814..3284)
                     /locus_tag="OO590_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6636358.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3400..3951)
                     /gene="maa"
                     /locus_tag="OO590_000890"
     CDS             complement(3400..3951)
                     /gene="maa"
                     /locus_tag="OO590_000890"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR6636359.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4123..4341)
                     /gene="hha"
                     /locus_tag="OO590_000891"
     CDS             complement(4123..4341)
                     /gene="hha"
                     /locus_tag="OO590_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6636360.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4367..4741)
                     /gene="tomB"
                     /locus_tag="OO590_000892"
     CDS             complement(4367..4741)
                     /gene="tomB"
                     /locus_tag="OO590_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6636361.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5287..8436)
                     /locus_tag="OO590_000893"
     CDS             complement(5287..8436)
                     /locus_tag="OO590_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6636362.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8459..9652)
                     /gene="acrA"
                     /locus_tag="OO590_000894"
     CDS             complement(8459..9652)
                     /gene="acrA"
                     /locus_tag="OO590_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6636363.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9794..10441
                     /gene="acrR"
                     /locus_tag="OO590_000895"
     CDS             9794..10441
                     /gene="acrR"
                     /locus_tag="OO590_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6636364.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10569..13931
                     /gene="mscK"
                     /locus_tag="OO590_000896"
     CDS             10569..13931
                     /gene="mscK"
                     /locus_tag="OO590_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6636365.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14143..14304)
                     /gene="rsmS"
                     /locus_tag="OO590_000897"
     CDS             complement(14143..14304)
                     /gene="rsmS"
                     /locus_tag="OO590_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6636366.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14318..14845)
                     /gene="priC"
                     /locus_tag="OO590_000898"
     CDS             complement(14318..14845)
                     /gene="priC"
                     /locus_tag="OO590_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6636367.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14915..15292
                     /locus_tag="OO590_000899"
     CDS             14915..15292
                     /locus_tag="OO590_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6636368.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15445..15996
                     /gene="apt"
                     /locus_tag="OO590_000900"
     CDS             15445..15996
                     /gene="apt"
                     /locus_tag="OO590_000900"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6636369.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16125..18050
                     /gene="dnaX"
                     /locus_tag="OO590_000901"
     CDS             16125..18050
                     /gene="dnaX"
                     /locus_tag="OO590_000901"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6636370.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17383..17447
                     /gene="dnaX"
                     /locus_tag="OO590_000901"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18103..18432
                     /locus_tag="OO590_000902"
     CDS             18103..18432
                     /locus_tag="OO590_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6636371.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18432..19037
                     /gene="recR"
                     /locus_tag="OO590_000903"
     CDS             18432..19037
                     /gene="recR"
                     /locus_tag="OO590_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6636372.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19147..21021
                     /gene="htpG"
                     /locus_tag="OO590_000904"
     CDS             19147..21021
                     /gene="htpG"
                     /locus_tag="OO590_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6636373.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21202..21846
                     /gene="adk"
                     /locus_tag="OO590_000905"
     CDS             21202..21846
                     /gene="adk"
                     /locus_tag="OO590_000905"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6636374.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21978..22940
                     /gene="hemH"
                     /locus_tag="OO590_000906"
     CDS             21978..22940
                     /gene="hemH"
                     /locus_tag="OO590_000906"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6636375.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22937..23897)
                     /gene="aes"
                     /locus_tag="OO590_000907"
                     /pseudo
     CDS             complement(22937..23897)
                     /gene="aes"
                     /locus_tag="OO590_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24049..25353
                     /gene="gsk"
                     /locus_tag="OO590_000908"
     CDS             24049..25353
                     /gene="gsk"
                     /locus_tag="OO590_000908"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6636376.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25486..27162)
                     /locus_tag="OO590_000909"
     CDS             complement(25486..27162)
                     /locus_tag="OO590_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6636377.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGKIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27400..28620)
                     /gene="fsr"
                     /locus_tag="OO590_000910"
     CDS             complement(27400..28620)
                     /gene="fsr"
                     /locus_tag="OO590_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6636378.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSQGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28838..30490
                     /gene="ushA"
                     /locus_tag="OO590_000911"
     CDS             28838..30490
                     /gene="ushA"
                     /locus_tag="OO590_000911"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6636379.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30527..31006)
                     /gene="ybaK"
                     /locus_tag="OO590_000912"
     CDS             complement(30527..31006)
                     /gene="ybaK"
                     /locus_tag="OO590_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6636380.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31210..32004)
                     /locus_tag="OO590_000913"
     CDS             complement(31210..32004)
                     /locus_tag="OO590_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6636381.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32142..32483
                     /locus_tag="OO590_000914"
     CDS             32142..32483
                     /locus_tag="OO590_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6636382.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32698..35202)
                     /gene="copA"
                     /locus_tag="OO590_000915"
     CDS             complement(32698..35202)
                     /gene="copA"
                     /locus_tag="OO590_000915"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6636383.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35464..36396
                     /gene="glsA"
                     /locus_tag="OO590_000916"
     CDS             35464..36396
                     /gene="glsA"
                     /locus_tag="OO590_000916"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6636384.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36399..37691
                     /locus_tag="OO590_000917"
     CDS             36399..37691
                     /locus_tag="OO590_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6636385.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37816..38223
                     /gene="cueR"
                     /locus_tag="OO590_000918"
     CDS             37816..38223
                     /gene="cueR"
                     /locus_tag="OO590_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6636386.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38224..38682)
                     /locus_tag="OO590_000919"
     CDS             complement(38224..38682)
                     /locus_tag="OO590_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6636387.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38679..39596)
                     /locus_tag="OO590_000920"
     CDS             complement(38679..39596)
                     /locus_tag="OO590_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6636388.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39742..40419
                     /gene="fetA"
                     /locus_tag="OO590_000921"
     CDS             39742..40419
                     /gene="fetA"
                     /locus_tag="OO590_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6636389.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40406..41185
                     /gene="fetB"
                     /locus_tag="OO590_000922"
     CDS             40406..41185
                     /gene="fetB"
                     /locus_tag="OO590_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6636390.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41248..42102)
                     /gene="cnoX"
                     /locus_tag="OO590_000923"
     CDS             complement(41248..42102)
                     /gene="cnoX"
                     /locus_tag="OO590_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6636391.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42163..42972)
                     /gene="ybbO"
                     /locus_tag="OO590_000924"
     CDS             complement(42163..42972)
                     /gene="ybbO"
                     /locus_tag="OO590_000924"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6636392.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42962..43584)
                     /gene="tesA"
                     /locus_tag="OO590_000925"
                     /pseudo
     CDS             complement(42962..43584)
                     /gene="tesA"
                     /locus_tag="OO590_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43555..44241
                     /locus_tag="OO590_000926"
     CDS             43555..44241
                     /locus_tag="OO590_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6636393.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44238..46652
                     /locus_tag="OO590_000927"
     CDS             44238..46652
                     /locus_tag="OO590_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6636394.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47364..48458)
                     /gene="mnmH"
                     /locus_tag="OO590_000928"
     CDS             complement(47364..48458)
                     /gene="mnmH"
                     /locus_tag="OO590_000928"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6636395.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48527..49453)
                     /gene="allS"
                     /locus_tag="OO590_000929"
     CDS             complement(48527..49453)
                     /gene="allS"
                     /locus_tag="OO590_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6636396.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49683..50165
                     /gene="allA"
                     /locus_tag="OO590_000930"
     CDS             49683..50165
                     /gene="allA"
                     /locus_tag="OO590_000930"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR6636397.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50243..51058
                     /gene="allR"
                     /locus_tag="OO590_000931"
     CDS             50243..51058
                     /gene="allR"
                     /locus_tag="OO590_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR6636398.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51148..52929
                     /gene="gcl"
                     /locus_tag="OO590_000932"
     CDS             51148..52929
                     /gene="gcl"
                     /locus_tag="OO590_000932"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6636399.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALNSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52942..>53214
                     /locus_tag="OO590_000933"
     CDS             52942..>53214
                     /locus_tag="OO590_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6636400.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12821 a        13709 c        14020 g        12664 t
ORIGIN      
        1 ccgcccggag agggttagta gttccagcga taacgctgca agtcgatttg cccgcttccc
       61 gataccatca caccttctgc cagtaatgcc tgtcgctgac gctgtaaatc cggcccggtt
      121 agcgaaattg tgccatggcg attaaccacc cggtgccagg gtaaggtgct gccttcaggg
      181 agacgcttta acacaccgcc cacctggcgc gcggcgcggg gcgatcccgc cagtttcgcc
      241 acatcaccgt aagtggtgac atagccttcg ggaatagcgg cgacgatttg ccagacgcgt
      301 tggggaaatg aatcttcttt ttccatcttt tcttcctgag gtaatttttc agcataatct
      361 ggaaaaacgc ccgagtgaag tcgcattgcg caagaaacca gcatctggca cgcaatgggt
      421 tgcaattagc cggggcagca gtgataatgc gcctgcgcgt tggttttcaa cgctctcaat
      481 gggggctctg ttggttctcc cgcaacgcta ctctgtttac caggtcaggt ccggaaggaa
      541 gcagccaagg cagatgacgc gtgtgccggg atgtagctgg cagggccccc acccatttct
      601 gcctcccacc gtttcgtcaa aaaatcccag catggctaaa ctttaaccac aactgacgtc
      661 gcaagaattg tctggctgcg cagtacgctt cggaggtatg tctgatgaag tatgttgatg
      721 gttttgtggt tgccgttcct gccgataaaa aggatgccta tcgggaaatg gccgctaagg
      781 ccgcgccatt gtttaaagag tttggcgcgc ttcgtattgt cgaatgctgg gccagcgatg
      841 taccggatgg caaagtgacc gattttcgta tggcggtgaa agcggaagag aatgaagagg
      901 ttgtctttag ctggattgaa tacccttcaa aagaggtccg cgacgcagct aatcaaaaga
      961 tgatgtcgga cccacggatg aaagagttcg gcgagtccat gccgtttgac ggcaagcgaa
     1021 tgatctatgg cggattcgag tcaatcatcg acgaataggt agcgtgacgg gctgtgcggc
     1081 gtgccgtcag cccaaaaatt cacaaatgtt gctcggccca gcgtaagaaa tcttctttcg
     1141 gtaatgcctt gctgtagagc cagccctgac cgtagtgcac gccatgctgg tgtagccact
     1201 cttcttgttt actggtttcg attccctccg ctaccatttt cagtttcagt gtttttgcca
     1261 tttcgatgat atgcggcgtg acatttttat attccagcgc atcaacgaaa gatttatcga
     1321 tcttcagaat gtcgacatcc agatcctgta aataacttaa acttgaatac cccgtaccaa
     1381 aatcatcaag ataaatttca tggcccgcct cccggtagcg agaaattatc ggggcgctgg
     1441 ttttcggatc ggcaaactcg cgttcagtga gttcaagcgc gatctgtctg ggattaacct
     1501 gatagtgatt gatcatttca cgcagcaatt gcgggatttt ttccgaggtg agcacggtgg
     1561 attcaagatt gatcgaaata tgctgctgtg gatgctgacg caagcagtcg cccatatctt
     1621 caaagacgct tcttataatc agtagcgtca atggctcaga aaggcccgtt tgctgtgcca
     1681 gcggaataaa actatctggt gacaaccaac taccgtctgt ctgcggccag cgcgccagtg
     1741 cctcagcacc gacaattttg ccattggcta aggagacaat cggctgatag tgcacgcaaa
     1801 tatcacgatt ttcgatagca tcctgcagcc gatgatgcgg tgactggata cggcgcaaaa
     1861 tacgcagcac aaacatcgct gccagcaggc caatcaccaa cccgagcggt aaccaaataa
     1921 agacttgccg atgccagcct ttctgcaaca ttttcgttga agcccaggtg atgatcgaaa
     1981 tattcatctc cgggaagggc aggatatcgt aaatgatccc attattttcg atatgctcac
     2041 cgggtgtttt ttgtagcctg gtaataattc cctgagcaat ttcatcgctg ctggttatga
     2101 caacgttatg ggcattgcca ataatggcgg catcaatttg ccatgagcta tagggaatga
     2161 catcaataaa ggaagcgggg tcgatcatga cgacataatg tgccgttccc atggcgacca
     2221 tgtaacggat aatgcctaaa tcgttatgcg atgttaacca gacacgataa ccatctttcg
     2281 aaattttacc tggctcggga aaggtatcgg gcggactttc atgctccaga gacgaacact
     2341 ggggaacgtt gttatcgata taaaccactt cctgaatata gcgataactg taagataccc
     2401 gacgcatttc catgagatgg gcttcgctac aggcagcgcc ttgccatctt tccagctcct
     2461 gcagcgcatc tttcccttgt gtcgccacct tattggctcg aatagcgacg cgggaggaat
     2521 aggtatccag ctcttcaata aacgatgttt ctacctgctg atgggccagc cagatgctta
     2581 agccgacagg cagcaatact gaaagaatca gtactcccga aataaggccg accagatgtc
     2641 gtgttctcac cagcatatcc ttattaacac agtgtaggga aagtatatct gattgtggaa
     2701 aaaggctggc aggagataag gaggtagagc tggtatttca tgaagtgatg ggtggaaacg
     2761 atcattgtca tttgaccagg cgcagagctt tacgcctggt atgtaaccct aacttaagat
     2821 gttgattcgg cgcgagcgag ggtaaaaata tcgtaaaaag acagttcacc tttacggacg
     2881 atcatttttt gcagttgttc cttatgaaca tccgcgacac gcggagagtt caagatggct
     2941 tcaaccagcg cggcaggtac aaagcgcgtg ttgtaccatt cgccgttata gcaaacgcgg
     3001 aaatccagca cgtcgatatc ttcatagcgg tagcgtgggt agacatcgaa aaagaagaaa
     3061 ccattaagca ggataaataa tacaaccagt agccagacag agcctgacag cgtttcggac
     3121 tgcaacatca ccgccagggt ggcaaaaaaa gcaacgtaca taccgataaa cagccccgga
     3181 tgtttacgga taaaactgat actaaagcgg ggtttgttgt cgcgtttttc gcgggtattc
     3241 agagactcaa tcgtttcggt cagcaggcgt tgtatttcgg tcatcgtttg ccttcgtggt
     3301 atctgcaact ttccagggaa caccctattt taacgtggga atagtagagg ggaagtaaca
     3361 gatctaccac aattttgcat aacagttgcg aaaaaccgat tacaattttt taattattct
     3421 ggctggatta ccgcccacga caacgttgtc cgggacacct tttgtgacaa ctgcacctga
     3481 agctaccacg acgttatcac caatggtcac accagggtta atgaccgcgc gtccgccaat
     3541 ccagacgtta ttaccgatgg tgacgggttt ccccagttca gcaccgctat tacgtgctac
     3601 agggtcgatg ggatgtgttg ccgtgtagat atgaacgcct ggtgccaaca tacagttatc
     3661 accgatgcga atagggcaga catcaagcat cacgcaatcg aagttggcga aaaaattatt
     3721 gccgagaaaa atgttatagc cataatcaca gcgaaacgtt ggctcaatat aagcctctgt
     3781 cacctgaccg aataaatcag cgagaatttg ctggcgtaat gtgtgctctt ccgctaggga
     3841 atgattgtat cggtgaataa gctgacgagc gcgcaggcga tcgcgagata acgtctcatc
     3901 tgccgagcga tacaactcac cagcaatcat cttttctttt tctgtgctca ttactgacct
     3961 ccggttgctg aaaatgcggc aacacagtaa gacgaaaagt cagtgaagag aacgttctca
     4021 ttacatctgt gtgataaaga tcacataggg tatgtcttca tggcgaaaaa gtataaaatt
     4081 cttaataaac agccggttat agctccgaaa gcgaattatt tattagcgaa taaatttcca
     4141 tactgaggaa gggatcttgt cgtacagttt attcatggtc aattcggcga ggcggtgatc
     4201 tgcggctgag taaaataccg ccagttcatt atctgataat tcgtatttat ttttctcgat
     4261 aacacgctcc agcgtgtcaa ttgtctggca acgacgtaaa cgcattaaat aatcggtttt
     4321 tgttaaaggt ttttcggaca tacttctacc catggttgta ataattctaa caagataaac
     4381 tcgcaggatt ctctttcgtc gcattgacaa aacaacggaa tagtcgatta cctgacttcc
     4441 gccatttctg aagagcctgc atattaatac cataactgct gaacaacata aaggtgtcat
     4501 ccagatattc gtcgatctgc tcaatgagct tattgtcttc attatactta attttgtaat
     4561 taagtgcgaa ggtcgcaata tgctcaatca gttcattcaa ctggaggttg atcgccgagg
     4621 ttgggtcgtt aacccagcca tgattgcttt cttcaaggtt tgcaagacag tcatgataca
     4681 gggtttcaca gagaaactta agctgtgcga tatcatgtct tttgggtgag tattcatcca
     4741 taacgcgtcc ccttcttagt ggttgaacta acggacacct ttcgggatgg aaaaaactta
     4801 ctgacctaga cttgccctct cgtttgttag cttaactata agccactctt tgcaggtttt
     4861 catcgcatta ttacgaaaca ttacaattag gaaggaatcg ggacaggact cactttttca
     4921 tactagcact aacccgcagc aggtaaaagc agtttgctcg aactgttcat aacatttcag
     4981 gattatcgcc agatgaaatt ggtgacacta ataccaggat tgctctgaat atgacgtaat
     5041 aaccgaggaa tgaataaaga attaccgcaa ataattaaga atagcctcta aatgattatg
     5101 gattattaaa tctattagcg ctacttaata taaattaact aacaatcagt attttttatg
     5161 aattttatac gtggttaata ctggttttcg tatgagatcc tgagttggtg gttcaattac
     5221 tccttaatgt tcgtaggtta tgcataaaaa aggccgctta cgcggcctta gtgattacat
     5281 gttgtatcaa tgatgatcga cagtatggct gtgctcgata tcttcattct tgcggctaaa
     5341 gcggcggcga accaccacaa agaataccgg aacgaagaag attgccagta ccgttgcggt
     5401 caccatcccg cccattacac cggtacctac tgcgttctgc gcgccggaac cagcaccagt
     5461 actgataacc agcggcataa cgccgaggat aaacgccagc gaggtcatca ggatcggacg
     5521 taaacgcatc cgcaccgcat caagcgtcgc ttcaatcaga cctttacctt ctttatccat
     5581 caagtctttg gcgaattcga cgataagtat cgcgttcttc gccgacaacc caatggttgt
     5641 gagcagtcct acttggaagt aaacgtcatt ggtcaggcca cggaaggtgg cagccagcaa
     5701 cgcaccgata acccccagcg gaacgaccag cataacggag aacggagtcg accagctctc
     5761 gtacagcgcc gccagacaca ggaatacgac aatcaacgaa accgcgtaca gtgaaggtgc
     5821 ctggttgccg gagagacgtt cctgatagga catccccgtc cagtcatagc caacaccggt
     5881 aggcagtttg ctcgccagtt gttccatcag ctccattgct tcaccggtac ttttacccgg
     5941 tgccgcctgg cctaagattt ccatggatgg caggccgttg taacgttcca gacgcggcga
     6001 accgtactcc caacgagaag aggagaacgc cgagaatggc accatctgac catcagcagc
     6061 acgaacatac cagtcgccga tatcatccgg cagcatacgg tatttcgctt ctgacatgac
     6121 gtaaactttc ttcacacgac cgcggtcgat aaagtcgttc acatagctac cgccccatgc
     6181 agcgcccaga gtggtgttaa tgtcgttgat agaaacaccc agcgcctgcg ctttttcctg
     6241 gtcgatatca atcttaaact gcggggtatc ttccagaccg tttggacgta cgctggtcag
     6301 catatcaggg tgcttcgctg cttctgcaag cagctggtta cgcgcctgag tcagtttttc
     6361 gtgaccaagg ccagcctggt caatcagctc aaagtcaaag ccggttgcag tacccagttc
     6421 cacgattgcg ggcaggttaa aggcgaaaac catcgcatct ttgatttgcg agaaagcgcg
     6481 tgttgcacgc atggtaatcg cttcaacttt gttttcttcg cccggacgat cggcccagtc
     6541 cttcaaggaa acgaacgcaa taccggtatt ctgaccacgt cccgcaaagc cgaagccgtt
     6601 aacggcgaac accgactcaa cgttgttttt ttctttggtc agatagtaat gcgttacctc
     6661 attgagcact ttctgcgtac gttcctgcgt tgcacctgct ggcagctgaa ccatggtcat
     6721 aaatacgccc tggtcctcat ctggcaagaa ggagcttggc agacgcacga acagataggc
     6781 catgccgacc acgatgatca gatacagcac taggtaacgc cccgtactgc gcagaatacc
     6841 gcctacgctg tcggtgtagt ggtgcgtgct cttctcgaac atgcggttaa accagccgaa
     6901 gaagcctttt ttaccttccc cgtgatcgcc tttggcaatc ggtttcagca tggtggcaca
     6961 aagagccgga gtcaggatca acgctaccag taccgacagc gccattgctg aaacaatggt
     7021 aatagagaac tgacgataga tagcaccagt agaaccgcca aagaaggcca tcggtacgaa
     7081 taccgccgac agtaccatcg cgataccgac cagagcgccc tgaatctgcc ccatcgactt
     7141 acgggtagct tcttttggcg gcaaaccttc ttccgccata acacgctcca cgttttctac
     7201 cacaacgata gcgtcatcca ccaacaagcc gatggcgagc accatcccga acattgttag
     7261 cgtgtttatc gagaagccaa aggcggcaag gacggcaaag gtcccgagca ataccaccgg
     7321 tacggcaatg gtcggaatca acgtcgcgcg gaagttctgc aggaacagat acataaccag
     7381 gaacacgagg atgatcgctt cgaccagcgt tttaaccact tcgtgaatag agattttcac
     7441 gaacggcgtg gtgtcgtacg ggtaaacaat tttcagaccc gacgggaaga acggttccat
     7501 cttcgccagt tcagcacgga ttgccgcagc ggtatccagc gcgtttgcac cggtcgccag
     7561 cttgatcccc agaccggaag ccggttggcc gttaaactct gcgatgatgt cgtagttctc
     7621 accacccagc tcaatcttcg ccacatcacg cagcagtacg cgggaaccat cctgattcac
     7681 tttcagcagg attttgccga actcttcagt agaggtcaga cgcgtctgag caataataga
     7741 ggcgttaagc tgttggcctt tcaccggcgg cgtaccaccg agctgacccg ccgcaacctg
     7801 ggcgttctgc gctttgatgg cggtaatgac atcaaccggc gttagctgga atttgttcag
     7861 ctcattcggg ttcatccaga tacgcatcgc gtactgtgaa ccgaacaact gaacatcacc
     7921 cacgcccgac gtacggctga tggcatcttt catattcgcc gctacgtagt cagagatatc
     7981 ctcctgcgtc atggtgccat cggtgttgat aacgccgaca accatcagga agctgctgga
     8041 tgatttctca acgctcaccc cttgctgctg aacttcttgc ggcagcaacg gcatcgccag
     8101 ctgcagtttg ttctgcacct gaacctgcgc gatatccgca tcagtaccag actcaaaggt
     8161 cagggtgatc tgcacggtac ccgtggagtc actgttagag gacatgtaca tcaggttatc
     8221 gataccgttc atattctgtt cgataacctg tgtcaccgtg tcctgcactg ttttcgcatc
     8281 agcgccgggg taggaggcgg agatcgttac tgccggcggt gcaatcgtag gatattgtgc
     8341 caccggcagt ttgaggatcg ccagcccccc tgccaacatg atgataatgg cgatcaccca
     8401 cgcaaaaatc gggcgatcga taaagaaatt aggcatgtct taacggctcc tgtttaagtt
     8461 aagacttgga ctgttcaggc tgagcaccgc ttgcggcttg ctggttatta tcagcggtaa
     8521 cttcttgtgc ttttacctgg acaccaggac gcactttctg cagcccactt attactacgc
     8581 gatcgcctgc tttcagacct tctgtcacca gccacttatc gccaatagcc tggcttgcaa
     8641 cgatcggacg ggtttccact ttgtcatccg cgccaactac cagtacggtg gcatcaccac
     8701 gcggcgtacg ggttacgccc tgttgcggga ctaaaatagc gtttggatta agcccttctt
     8761 ccagacgtgc gcgcacgaac atacccggca gcagagtgtg atccgggttc gggaagatag
     8821 cgcgtagggt gatagaccca gtggtctgat caacggtaac gtcagagaat tccagcgtac
     8881 cgtcctgcgg gaacttaatg ccgtcactgg tgatcagcga cactttggct ttgccgttct
     8941 cttgtttcag cgtgccattc gccagttcct gtttcaggcg caggaagtcg ttgctggact
     9001 gggtcacatc aacgtagatc ggatcaagtt gctgcacggt tgccagcgca gtcgcctgac
     9061 cgttctgtac caatgcgcct tccgtcacgt tcgacttacc aatgcgaccg ctaatcggag
     9121 aggtgacttt ggtgtaagcc agattgatcc gcgcagtttc aacggcagct ttcgccgcag
     9181 ttaccgcagc attcgcctgt tgcgcatcag ccagagcctg atcgtaccct tgcttactga
     9241 tgtactgagt accgagcagt ttctgataac gattcaccgt caattgcgcg atattggctg
     9301 cagcctgggc tttcgccaga tcacctttcg cactgtcgta tgtcgcctga taggtcgcag
     9361 gatcaatctg atagagagag acaccagctt cgatgtcgct accttctttg aaattacgct
     9421 tcaggataat cccgctaact tgaggacgaa cttctgcgat ccggtaggca ctggtgcgac
     9481 ccggaagctc ggttgtgatc tgcagaggtt cagttttgac tgttactacg ccaacggcgg
     9541 gcatctgctg gccaccttgt tgggcctgtt tgtcgtcaca tcctgttagg gctaagctgc
     9601 ctgagagcat cagaacgacc gccagaggcg taaaccctct gtttttgttc atatgtaaac
     9661 ctcgagtgtc cgatttcaaa ttggtcaatg gtcaaaagtt aataaaccca ttgctgcgtt
     9721 tatattatcg tcgtgctatg gtacatacat tcacaaatgt atgtaaatct aacgcctgta
     9781 aattcacgaa catatggcac gaaaaaccaa acaagaagcg caagaaacgc gccaacacat
     9841 cctcgatgtg gctctacgtc ttttctcaca gcagggggta tcatccacct cgctgggcga
     9901 gattgcaaaa gcagctggcg ttacgcgcgg tgcaatctac tggcatttta aagacaagtc
     9961 ggatttgttc agtgagatct gggaactgtc agaatccaat attggtgaac tagagcttga
    10021 gtatcaggca aaattccctg gcgatccact ctcagtatta agagagatat taattcatgt
    10081 tcttgaatcc acggtgacag aagaacgacg tcgattattg atggagatta tattccacaa
    10141 atgcgaattt gtcggagaaa tggctgttgt gcaacaggca caacgtaatc tctgtctgga
    10201 aagttatgac cgtatagaac aaacgttaaa acattgtatt gaagcgaaaa tgttgcctgc
    10261 ggatttaatg acgcgtcggg cagcaattat tatgcgcggc tatatttccg gcctgatgga
    10321 aaactggctc tttgccccgc aatcttttga tcttaaaaaa gaagcccgcg attacgtcgc
    10381 catcttactg gagatgtatc tcctgtgccc cacgcttcgt aatcctgcta ctaacgaata
    10441 accctgaatc tgactccagg atttttcctg gacattttcg tcgttgctat tctggttcac
    10501 tgcgtcgtga tattcttgcg gtttgacttt ttcaggtcgt tcttcaggtt cagaaacctt
    10561 cattcatcat gactatgttc cagtattaca aacgatcacg gcattttgtt ttttcagcat
    10621 ttattgcttt tgtttttgtc ttgttatgcc agaacacggc gtttgcgcgg gcgtcatcga
    10681 atggtgatct gccgacaaaa gcggacctgc aggcgcaact tgactcacta aataaacaaa
    10741 aagatctttc tgctcaggac aaactggtgc agcaggatct gacagataca ttagccaccc
    10801 tcgataaaat cgatcgcata aaagaagaga cagttcagct acggcaaaaa gtcgctgaag
    10861 cgccggaaaa aatgcgccag gcgaccgcgg cgttaacagc acttagcgat gtcgataacg
    10921 acgaagaaac gcgcaaaatt ctgagtacgc tttcgttgcg ccagctggaa actcgcgttg
    10981 cccaggcgct ggacgatttg caaaacgcac aaaacgatct ggcgtcttat aacagccagc
    11041 tggtttcgtt acagacgcag cccgaacgcg tgcaaaatgc gatgtataac gcttcgcagc
    11101 agctgcaaca aattcgcagt cgtctggatg ggactgatgt cggcgagact gccttacgtc
    11161 ccagccagaa agtgttaatg caggctcagc aggcgttgct gaatgcggag attgaccagc
    11221 agcgtaaaag cctggaaggg aacaccgtct tgcaggatac cttgcaaaag caacgtgatt
    11281 acgtgacggc gaacagcgcc cgtctggagc atcagttgca gctgttgcaa gaagcggtaa
    11341 acagcaagcg cctgacttta actgaaaaaa cggcgcagga agccgtctcc ccggatgaag
    11401 ccgcgcgtat tcaggctaat ccgctggtga aacaggaact ggaaattaac cagcagttaa
    11461 gtcagcgtct gattaccgcg actgaaaacg gtaatcagtt gatgcagcaa aacattaaag
    11521 tcaaaaactg gctggagcgg gcgctgcaat cggaacgcaa tattaaagag cagattgccg
    11581 tcctgaaggg cagcctgctg ttgtctcgta tcctttacca gcaacaacaa acgctgccct
    11641 cggcggatga actggaaaac atgaccaacc gcatcgcgga tttgcgtctc gaacagtttg
    11701 aagttaacca gcagcgtgat gcactcttcc agagcgatgc gttcgtcaac aaactggaag
    11761 aaggtcacac caacgaagtc aacagcgaag ttcacgatgc gttattgcaa gtggttgata
    11821 tgcgtcgcga attgctggat caactcaaca aacagttggg taaccagctg atgatggcca
    11881 ttaacctgca aatcaaccag cagcagttaa tgagcgtatc gaaaaacctg aaatccatcc
    11941 tgactcagca aatcttttgg gtgaacagta accgtccaat ggactgggac tggatcaaag
    12001 cgttcccgca aagcctgaaa gatgaattta agtcgatgaa aatcacggtg aactgggaaa
    12061 aagcctggcc tgccgttttt atcgctttcc tcgccggttt gccgctgctg ttgattgccg
    12121 ggctgatcca ctggcgtctg ggctggctga aagcgtatca acagaaactg gcttccgctg
    12181 tgggttccct gcgtaacgat agccagctca acacaccaaa agcgatcctt atcgacctga
    12241 tccgcgcgct gccggtgtgc ctgattattc tcgcggttgg cctgattctg ttgaccatgc
    12301 agctcaatat cagcgaactg ctatggtcgt tcagtaaaaa actggcgatt ttctggctgg
    12361 tgtttggcct gtgctggaag gtactggaga aaaatggcgt tgccgtgcgt cacttcggca
    12421 tgccggagca gcagaccagc cactggcgtc ggcaaattgt ccgcatcagt ctcgcgttgc
    12481 tgcctatcca tttctggtct gtggtggcag aactttcccc gctgcatctg atggatgatg
    12541 tgctggggca ggcgatgatt ttcttcaacc tgctgctgat tgccttcctg gtatggccaa
    12601 tgtgccgcga aagctggcgt gataaagagt cgcacaccat gcgactagtc actattaccg
    12661 tgctgtcgat tatcccgatt gcattgatgg tgctgactgc cacgggttat ttctacacca
    12721 cgctgcgcct ctccgggcgt tggattgaaa ccgtttatct ggtgatcatc tggaacttgc
    12781 tctaccagac ggtattgcgc ggcttaagcg tagcggcgcg gcgtatcgcc tggcgtcgtg
    12841 cgctggcgcg tcggcagaat ctggtgaaag agggcgcaga aggtgccgaa ccgccggaag
    12901 aacccaccat tgcactggag caagttaacc agcagacgct gcgtattacc atgttgctga
    12961 tgtttgcgct gttcggtgtc atgttctggg caatttggtc cgatttgatc accgtgttca
    13021 gctatctcga cagcatcacg ctctggcatt acaacggcac tgaagctggc gctgcggtgg
    13081 tgaaaaacgt caccatgggc agtctgttgt ttgcgattat cgcctcaatg gtggcctggg
    13141 cgttgattcg caacctgcct ggtttactgg aagtgctggt gctctcgcga ctgaatatgc
    13201 gccagggcgc gtcgtatgcc attactacca tccttaacta catcattatt gccgttggtg
    13261 cgatgacggt gttcggatcg ctgggcgtct cttgggataa actccagtgg ctggccgcag
    13321 cattatccgt aggtcttggt tttggtttac aagaaatttt cggtaacttc gtctccggtt
    13381 tgatcattct gttcgaacgt ccggtgcgta ttggcgatac ggtaaccatt ggtagcttct
    13441 cggggacggt aagtaagatc cgtattcgtg cgacaacgat taccgatttc gatcgcaaag
    13501 aagtgatcat cccgaacaaa gcgtttgtta ccgagcgtct gatcaactgg tcgttgactg
    13561 acactactac gcgtctggtg atccgtctcg gcgtggccta tggctccgat ctggaaaaag
    13621 tgcgtaaagt gttactgaag gcggcgactg agcacccaag ggtgatgcac gaaccaatgc
    13681 cggaagtctt ctttacggca tttggtgcca gcacgttgga tcatgagctg cgtctgtatg
    13741 tgcgtgaact gcgtgaccgt agccgtactg tcgatgagct gaaccgtact atcgatcagc
    13801 tgtgccgtga aaacgacatc aacattgcct ttaaccagct tgaagtgcat ctgcacaacg
    13861 agaagggcga tgaggtgacg gaagtaaaac gcgactacaa aggcgacgat ccgacaccag
    13921 cggttgggta ataaccaaag ggggcgacat ttagttgccc cgagattact gacaaagtgc
    13981 gcgttgttca tgccggatgc ggcgtgaccg ccttatccgg cctacgaaac cgcaagaatt
    14041 caatatattg caggagcggt gtaggcctga taagcgtagc gcatcaggca gttttgcgtt
    14101 tgcccgcaat cttaggggac atttagcggc cccatttatt tctcactttt ccgcctcatc
    14161 atcgtgcgtt aatttctttt cataatcacg ctttacaata tccagcgcgc gcagcacggt
    14221 actggcaggg atctgatttt cctccagcag cacaatcaaa tcgacagcca gtttgacatc
    14281 gtcaggggca ttttcaagtg acatactctt tccattgcta gcgggttaag cgcgctagcc
    14341 tgttttcgat tttttccagc gcatggcggc agcgtgccag gcgcgcttca taggcttcca
    14401 cttcacgatg cagcgtttgc tgttccacga gatcggtcac ccgcgccagg cgggctctgc
    14461 gttcggcaac catctcacgt agccgccgct caaaatcctg atgctgaata cgtttacgct
    14521 gccagcgggc aattttcggt ggcgcactgt cccactcgcg caatgaccag gcggcggctt
    14581 cacgcgcgat ggcttccagt tgtgccgcca gatgttccgc cagccaggcc acttgcggca
    14641 gctgttgctg ctcaactgca tgacgaagcg cagccagatt atcgcccgcc tcgtcgagac
    14701 aagcctgtag tgttgtcgca cgagtctgaa aaagatgcct gtcgaaacga gcacttagcg
    14761 tggcgaactg tgacaccggg gcacaacgct gacgcagcgt agcgagctgt ccttccagtt
    14821 tttccagcag cagggcggtt ttcacgataa gacctcaatg aaaatgataa ttgttatgct
    14881 aaagtagcca ctctgtaagc tgacaacact caatatgcaa cgaatcattt taatcatcat
    14941 tggctggctg gcggtagtgc tgggtacgct gggcgtggta ttaccggtat taccgacgac
    15001 gccgtttatc ctgctggcgg cctggtgctt tgcccgttct tccccgcgct ttcacgcctg
    15061 gttgctgtac cgctcatggt ttggcagcta tctacgtttc tggcagaaac atcatgcgat
    15121 gccgcgcggc gtcaaaccgc gggcgatttt gcttattttg ctcacgtttg ccatttctct
    15181 gtggttcgtc cagatgccat gggtgcgcat catgttgctg gtaattctcg cctgtttgct
    15241 tttctatatg tggcgaattc cggtgattga tgaaaagcaa gaaaagcact gaagcacaac
    15301 aatcgcagtt gcaattattg cgtacagcca gtacattctg gcgttttcga gcacaggcgc
    15361 aggcggtcaa aggttaaaca acggttactt ttgatacgtt taaaacgcgc cgtgagtacc
    15421 accgtaacaa gcaggcatac acttatgacc gcgactgcac agcagcttga gtatctcaaa
    15481 aatagcatca aaagcattca ggactaccca aaacccggca ttcttttccg cgatgtcacc
    15541 agcttactgg aagacccgaa agcttacgct ctcagcatcg acttgctggt tgagcgttac
    15601 aaaaatgcgg gcattaccaa agttgtcggc accgaagcgc gtggcttctt gtttggcgct
    15661 ccggtagctc tgggtctggg cgttggcttt gtaccggtcc gtaaaccggg caaactgccg
    15721 cgtgaaacca tcagtgaaaa ttacgacctg gaatacggca ccgatcagct ggaaatccac
    15781 gttgatgcca tcaaaccggg cgacaaagtt ctggtggtgg acgatctgct ggcaaccggc
    15841 ggcactatcg aagcgaccgt taaactgatc cgtcgtctgg gtggtgaagt ggctgacgct
    15901 gcgttcatta tcaacctgtt cgatctcggc ggcgaacagc gtctcgaaaa acagggcatt
    15961 accagctaca gccttgtccc gttcccgggc cattaattat cgccagtctt gtgctgccca
    16021 cgctacggac agcacaagat gtgcattcag cctcgccgtt ctgacggggc tgtgttagca
    16081 ttaccccttc gtgaatccac cttccagcgt ttcagagcct gccaatgagt tatcaggttt
    16141 tagcccgaaa atggcgccca caaacctttg ctgacgtcgt cggccaggaa catgtgctga
    16201 ccgcactggc gaacggcttg tcgttagggc gtattcatca tgcttatctt ttttccggca
    16261 cccgtggcgt cggaaaaacc tctatcgccc gactgctggc gaaggggcta aactgcgaaa
    16321 ccggcattac cgcgacgccg tgcggcgtgt gcgataactg tcgtgaaatc gagcaggggc
    16381 gctttgtcga tctgattgaa atcgacgccg cctcgcgcac caaagttgaa gatacccgcg
    16441 acctgctgga taacgtccag tacgctccgg cgcgtggtcg tttcaaagtt tatctgatcg
    16501 acgaagtgca tatgctgtcg cgccacagct ttaacgcact gttaaaaacc cttgaagagc
    16561 cgccggagca cgttaagttt ctgctggcga cgaccgatcc acagaaattg ccggtgacga
    16621 ttttgtcacg ctgtctgcaa tttcatctca aggcgctgga tgtcgagcaa attcgccatc
    16681 agcttgagca catcctcaat gaagaacata tcgctcacga gccgcgggcg ctgcaattgt
    16741 tggcgcgcgc cgctgaaggc agcctgcgag atgccttaag tctgaccgac caggcgattg
    16801 ccagcggtga cggccaggtt tcaacccagg cggtcagtgc gatgctgggt acgcttgacg
    16861 acgatcaggc gctgtcgctg gttgaagcga tggtcgaggg caacggcgag cgcgtaatgg
    16921 cactgattaa tgaagccgct gcccgtggta tcgagtggga agcgttgctg gtggaaatgc
    16981 tcggcttgtt gcatcgtatt gcgatggtac aactttcgcc tgctgcactt ggcaacgaca
    17041 tggccgccat cgagctgcgg atgcgtgaac tggcgcgcac cataccgccg acggatattc
    17101 agctttacta tcagacgctg ttgattggtc gcaaagaatt accgtatgcg ccggaccgcc
    17161 gcatgggcgt tgagatgacg ctgctgcgcg cgctggcgtt ccatccgcgt atgccgctgc
    17221 ctgagccaga agtgccacgc cagtcctttg cacccgtcgc gccaacggca gtaatgacgc
    17281 caaccctggt gccgccgcaa tcagcgccgc agcaggcacc gactgtaccg ctcccggaaa
    17341 ccaccagcca ggtgctggcg gcgcgccagc agttgcagcg cgtgcaggga gcaaccaaag
    17401 caaaaaagag tgaaccggca gccgctaccc gcgcgcggcc ggtgaataac gctgcgctgg
    17461 aaagactggc ttcggtcacc gatcgcgttc aggcgcgtcc ggtgccatcg gcgctggaaa
    17521 aagcgccagc caaaaaagaa gcgtatcgct ggaaggcgac cactccggtg atgcagcaaa
    17581 aagaagtggt cgccacgccg aaggcgctga aaaaagcgct ggaacatgaa aaaacgccgg
    17641 aactggcggc gaagctagcg gcagaagcca ttgagcgcga cccgtgggcg gcacaggtga
    17701 gccaactttc gctaccaaaa ctggtcgaac aggtggcgtt aaatgcctgg aaagaggaga
    17761 gcgacaacgc agtatgtctg catttgcgct cctctcagcg gcatttgaac aaccgcggcg
    17821 cacagcaaaa actggctgaa gcgttgagca tgttaaaagg ttcaacggtt gaactgacta
    17881 tcgttgaaga tgataatccc gcggtgcgta cgccgctgga gtggcgtcag gcgatatacg
    17941 aagaaaaact tgcgcaggcg cgcgagtcca ttattgcgga taataatatt cagaccctgc
    18001 gtcggttttt cgatgcggag ctggatgaag aaagtatccg ccccatttga tcgtaagcac
    18061 agcttacgtt cgtcatcctt aacgtgattg agagagaaac ctatgtttgg taaaggcggt
    18121 ctgggtaacc tgatgaagca agcccagcag atgcaagaaa aaatgcagaa aatgcaggaa
    18181 gagatcgcgc agctggaagt taccggcgaa tctggcgcag gtctggtaaa agtgaccatc
    18241 aacggtgcac acaactgccg tcgcgtagag atcgacccga gcctgctgga agacgacaaa
    18301 gagatgctgg aagacctggt ggctgcagca ttcaacgacg cagcacgtcg tattgaagaa
    18361 acgcagaaag aaaaaatggc ctctgtatcc tccggaatgc agctgccgcc tggctttaag
    18421 atgccgttct gatgcaaacc agcccgctgt taacacagct tatggaagca ctgcgctgtc
    18481 tgccgggcgt tggcccgaag tcggcgcagc gtatggcgtt cacgctgctt cagcgcgatc
    18541 gtagcggcgg gatgcgtctg gcgcaggcgc tcacccgggc gatgtcggaa atcggccact
    18601 gcgccgattg ccgcactttc accgaacagg aagtctgtaa catctgttcg aatccgcgtc
    18661 gtcaggaaaa cggtcaaatc tgtgtggtgg agagtccggc ggacatctac gccattgagc
    18721 agacggggca gttttctggt cgttattttg tgttgatggg gcatctgtca ccgctggacg
    18781 gcatcggtcc ggatgatatc gggcttgatc gtctggaaca gcgtctggca gaggaaaaaa
    18841 tcactgaagt gatcctcgcc accaatccca cggttgaagg tgaagctacc gctaactaca
    18901 ttgccgagct ttgcgcgcaa tatgacgtgg aagccagccg aatcgctcat ggcgtaccgg
    18961 ttggcggcga actggaaatg gtcgatggca ccacgctgtc acactccctc gccgggcgtc
    19021 ataagattcg tttttaagca aacgagagca ggagcacctg ctctcgcttg aaattattct
    19081 cccttgtccc catctctccc tcatcctgtt tttaacctta aaatggcatt attgaggtag
    19141 acctacatga aaggacaaga aactcgtggt tttcagtcag aagtgaaaca gcttctgcac
    19201 ctgatgatcc attctctcta ttccaataaa gaaatcttcc tgcgtgagct tatctctaac
    19261 gcctccgatg cggcggacaa gctgcgtttc cgtgcgctat ctaacccgga cctgtacgaa
    19321 ggtgatggcg aactgcgcgt tcgtgtctct ttcgataaag acaagcgcac gctgaccatc
    19381 tccgataacg gcgtggggat gacccgcgac gaagtgattg accatctggg gactatcgct
    19441 aaatccggta ccaaatcatt cctcgaatcc ctgggttctg accaggcgaa agacagccag
    19501 ctgatcggtc agtttggtgt tggtttctac tctgcgttta tcgtggccga caaagtgacc
    19561 gtgcgtactc gcgcggcagg cgaaaaacca gaaaacggcg tcttctggga atcggctggc
    19621 gaaggtgaat ataccgttgc cgacatcacc aaagaagatc gtggtactga aatcaccctg
    19681 catctgcgtg aaggcgaaga cgagttcctc gatgactggc gcgtgcgttc catcatcagc
    19741 aaatactccg accatatcgc gctgccggta gagatcgaaa aacgcgaaga gaaagatggc
    19801 gaaaccgtta tctcctgggc aaaaatcaac aaagcgcagg cgctgtggac tcgtaacaag
    19861 tcggaaatca ccgatgaaga gtataaagag ttctacaaac acatcgccca cgactttaac
    19921 gatccgctga cctggagcca caaccgcgtt gaaggtaagc aggagtacac cagcctgctg
    19981 tacatcccgt cccaggctcc gtgggatatg tggaaccgtg atcataaaca cggcctgaaa
    20041 ctgtacgttc aacgcgtgtt tatcatggac gacgctgaac agttcatgcc gaactatctg
    20101 cgcttcgtgc gtggtctgat tgactccagc gatctgccgc tgaacgtttc ccgtgaaatc
    20161 ctccaggaca gcacggtaac gcgtaacctg cgcaatgcgc tgaccaagcg tgtgctgcaa
    20221 atgctggaaa aactggcgaa agacgacgcg gaaaaatacc agaccttctg gcaacagttt
    20281 ggcctggtac tgaaagaagg tccggcggaa gatttcgcta accaggaagc gatcgccaaa
    20341 ctgctgcgtt ttgcttctac ttataccgat tcttctgcgc agaccgtgtc tctggaagac
    20401 tacgtttccc gcatgaaaga agggcaggag aaaatctact acatcactgc agacagctat
    20461 gcggcagcga agagcagccc gcacctggaa ctgctgcgta agaaaggcat cgaagttctg
    20521 ctgctttccg accgcatcga tgagtggatg atgaactatc tgactgagtt cgacggtaaa
    20581 ccgttccagt cggtgtctaa agttgacgag tcgctggaaa aactggctga cgaagttgat
    20641 gagagcgcga aagaagcgga gaaagcactg actccgttca tcgaccgcgt gaaagctctg
    20701 ctcggcgagc gcgtgaaaga tgtccgtctg actcaccgtc tgaccgatac gccagcgatc
    20761 gtttcgaccg acgcggacga aatgagcact cagatggcga aactgttcgc tgcggcgggc
    20821 cagaaagtgc cagaagtgaa atacatcttc gaactgaacc cggatcacgt actggtgaaa
    20881 cgtgcggcag atactgaaga tgaagccaag ttcagcgagt gggtagaact gctgctggat
    20941 caggcgctgc tggcagaacg cggcacgctg gaagatccga acctgtttat tcgtcgtatg
    21001 aaccagctgc tggtttcctg atgtcatgcc ggatgacctt tgtgtcatcc ggcatttttc
    21061 ttttcatcat ctgtactttc cgcaaattat ctcgccatta accgtttcag cctcaggtgc
    21121 ctttcttgag gcaatcgcct gttggtggta tcgtttatcg ctttttcaaa aaaatcgaca
    21181 cattttaagg ggattttcgc aatgcgtatc attctgcttg gcgctccggg cgcggggaaa
    21241 gggactcagg ctcagttcat catggagaaa tatggtattc cgcaaatctc cactggcgat
    21301 atgctgcgtg ctgcggtcaa atctggctcc gagctgggta aacaagcaaa agacattatg
    21361 gatgctggca aactggtcac cgacgaactg gtgatcgcgc tggttaaaga gcgcattgct
    21421 caggaagact gccgtaatgg tttcctgttg gacggcttcc cgcgtaccat tccgcaggca
    21481 gacgcgatga aagaagcggg catcaatgtt gattacgttc tggaattcga cgtaccggac
    21541 gaactgattg ttgatcgtat cgtaggccgc cgcgttcatg cgccgtctgg tcgtgtttat
    21601 cacgttaaat tcaatccgcc gaaagtagaa ggcaaagacg acgttaccgg tgaagaactg
    21661 actacccgta aagacgatca ggaagaaacc gtgcgtaaac gtctggttga ataccatcag
    21721 atgactgcac cgctgatcgg ctactactcc aaagaagcgg aagcgggtaa caccaaatac
    21781 gcgaaagttg acggtaccaa gccggttgct gaagttcgcg ctgatctgga aaaaatcctc
    21841 ggctaatcaa gccgcagcag atctgctttc gggcaggtct gctcatccac ctcaggcaat
    21901 acctctcaca gcaattagtt cttcttcctc acttttccgc tacaattatc aacaagttga
    21961 atcgataaga ggcggtaatg cgtcagacta aaaccggtat cctgctggca aacctgggta
    22021 cgcccgatgc ccccacacct gaagcggtaa aacgctatct gaaacaattt ttaagcgaca
    22081 gacgcgtggt tgatacctca cggttgttat ggtggccgtt gctgcgcggc gtgattttgc
    22141 cgctgcgctc gccgcgtgtg gcgaagctgt acgcctctgt ctggatggaa gacggctcgc
    22201 cgctgatggt ttacagccgc cagcaacagc aggcgttggc acaacgttta ccggatacgc
    22261 cagtggcgct gggcatgagc tacggctcgc catcactgga aagcgccgtc gatgaactcc
    22321 tggcagagca tgtagatcat attgtggtgc tgccgcttta tccgcaattc tcctgttcta
    22381 cggtcggtgc ggtatgggat gaactggcac gcattctggc gcgcaaacgt agcattccgg
    22441 ggatatcgtt tattcgtgat tacgccgata accacgatta cattaatgca ctggcgaaca
    22501 gcgtacgcgc ttcttttgcc aaacatggcg aaccggatct gctgctgctc tcttatcatg
    22561 gcattcccca gcgttatgca gatgaaggcg atgattaccc gcaacgttgc cgcacaacga
    22621 ctcgcgaact ggcttccgca ctggggatgg taccggaaaa agtgatgatg acctttcagt
    22681 cgcgctttgg ccgggaaccc tggctgatgc cttataccga cgaaacgctg aaaatgctcg
    22741 gagaaaaagg cgtaggtcat atacaggtga tgtgcccggg ctttgctgcg gattgtctgg
    22801 agacgctgga agagattgcc gagcaaaacc gtgaggtctt cctcggtgcg ggcgggaaaa
    22861 aatatgaata tattccagcg cttaatgcca cgccggaaca tatcgaaatg atggctaatc
    22921 ttgttgccgc gtatcgctaa agctgagcgg taaagaactg agcgccgtcg cgaagagcct
    22981 cgtcggcggt tttcatcatc cgtgaataat gcaaaaaggc atgcagcgta cctgggtaga
    23041 gtttgaactc acagggctgc tgatgcgccg ctaacgtctg gtaaagcaga cggctgtcat
    23101 ccagcagcgg atcgaactcc tccccggcaa taaaacaggg cggaacttcg cgagtgagat
    23161 cattattaaa cagacagtaa tacggtgact cgcggtccgc gtcgttgctt aaatacgcct
    23221 cttcatacat ctgcaaatcc tgttgcgtta agccatccca gacaccgccc aacagacgac
    23281 gagtcacgga atcccgtaat ccgtaaagcc cataccacag caaaacaccc gcaattttac
    23341 cgcaatcgat ctgtttatca cgcaaccaca acgcactggc gagcgccagc atggcacctg
    23401 cggaatcacc ggcaaagcca atgcgggaca tattgatttg ataatcctcc gcctgctggt
    23461 ggaaataaca acaagcagcc acaatttcct ctatcgcttg tggaaaacgc gcttcaggtg
    23521 aaagggtgta atcaatacca atcaccgtac attggctgta gcttgccagc aggcgcatga
    23581 tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc ttccatgcaa ataaaatagc
    23641 gtcgccgggc tatctggctg cggacaaaag agacgtgttt ccacctgccc atattttgtt
    23701 ggaaccatgt aagctctggt tgccatttct ggagcgcccg cattccagaa tcggcgctca
    23761 agcgtgtaat actgtcgttg ctcagcaatc gttcccgttg cgggccagga cggtaaatcc
    23821 ggctgaagag tattcacaac ggtcttcatt tcagcagaaa taaggtccag aacaggtagt
    23881 ttgttttccg gcttcataca aaaactcctt tcaaattacg tcattgtaag gaaccactgc
    23941 catgaaaatg cgatcccgcc tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc
    24001 agattgtgtt aacattcgcc gctcagttaa ccacccgtaa aaacaaccat gaaatttccc
    24061 ggtaaacgta aatccaaaaa ttacttccct gtaaatgcac gcgatccgct gcttcagcaa
    24121 ttccagccag aaaacgaaac cagcgccgcc tgggtcgtag gtattgacca gacgctggtc
    24181 gatattgaag cgaaagtgga tgatgaattc attgagcgtt atggattaag cgccgggcat
    24241 tcactggtga ttgaggatga cgtagccgaa gtgctttatc aggagctaaa acagaaaaac
    24301 ctgattaccc atcagtttgc gggtggcacc attggtaaca ccatgcacaa ctactcggtg
    24361 ctcgcggacg accgttcggt gctgctgggc gtcatgtgca gcaatattga aattggcagt
    24421 tatgcctatc gttacctgtg taacacttcc agccgtaccg atcttaacta tctacaaggc
    24481 gtggatggtc cgattggtcg ttgctttacg ctgattggcg agtccgggga acgtaccttt
    24541 gctatcagtc caggccacat gaaccagctg cgggctgaaa gcattccgga agatgtgatt
    24601 gccggagcct cggcactggt tctcacctca tatctggtgc gttgcaagcc gggtgaaccc
    24661 atgccggaag caaccatgaa agccattgag tacgcgaaga aatataacgt accggtggtg
    24721 ctgacgctgg gcaccaagtt tgtcattgcc gagaatccgc agtggtggca gcaattcctc
    24781 aaagaccacg tttctatcct tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa
    24841 agcgatccgt tgttggcatc tgacaaggcg ctggactggg tagatctggt gctgtgcacc
    24901 gccgggccaa tcggcttgta tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc
    24961 cagcatccgc tgctgccggg cgctatagcg gaattcaacc agtatgagtt tagccgcgcc
    25021 atgcgccaca aggattgcca gaatccgctg cgtgtctatt cgcacattgc gccgtacatg
    25081 ggcgggccgg aaaaaatcat gaacactaat ggagcggggg atggcgcatt ggcagcgttg
    25141 ctgcatgaca ttaccgccaa cagctaccat cgtagcaacg taccaaactc cagcaaacat
    25201 aaattcacct ggttaactta ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg
    25261 agctatcagg tactgaacca gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa
    25321 gacagcctgg aagagtctta ctgggatcgt taagttatcg tcggttcgta ggccagataa
    25381 ggcgttcacg ccgcatctgg catttggctc tcgatgcctg atgcgacgct ggcgcgtctt
    25441 atcatgccta catatttttc atattttaca tccggcaacc accgtttacc ccgtcaccac
    25501 ttcacccgcc ggtggcgttt ccagcagttc cagcatagta cgggcaattt cacgttcacc
    25561 catcactacc tgattcgcgc cacgttcggt gatatacgtc acttcatcgt cataatgggc
    25621 gcgggcgata atctcaatat ccgggttttt cgcgcgggca gacgccacaa tctcacccgc
    25681 ttcataaccg ttggggatcg tcaggatcag ccattttgca cattccagat gtgccagttg
    25741 cataatttct tcgttcgctg cattgcccaa tacagcgcgg accccacgct ctcgcagctc
    25801 atcaacacgg gttcgtgacg tctcaatcac caccagcgga atatcagagg cgagcaattt
    25861 ctcccccagc aggctgccta cacgaccgta acccaccaat agcgcatggt tgcaaatatc
    25921 cactgggatc tgtttctctt cttcgattgc ctcttccagc gtctgctctt ccagcgtttc
    25981 ggtcttcgcc agatatttct ccagtagtgc gaacagtacc gggttgagca taatcgacag
    26041 gatcgcccct gccagtacca ggttttgtcc ggcctgcggc agtaaattca atgccattcc
    26101 cagtcccgcc aggataaacg cgaactcacc aatctgcgcc aggctggcgg cgatggttaa
    26161 tgccgtacgt tgggagtgac caaacagacg gacaaggaac agggccgcga ccgatttacc
    26221 aaacagaata attgccagcg tcgccagcac tgccagcggt tgctgaatca gaattaacgg
    26281 atcaaacaac atcccgactg agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcaa
    26341 cgtatcgtgg gcggcacggt gactcaattc agactcgttc agtaccatcc cggcaaagaa
    26401 cgcaccgagt gcaaaggaga catcaaacag ctctaccgca ccaaaggcaa tccctaacgc
    26461 cagcgccagc actgataggg taaacagttc gcgagaaccg gttgccgcgc tgcgtgccat
    26521 aatccacggc accagacggc gacctaccag catcataatg gcgataaatg cgatcacctt
    26581 gccgatggtg atccccatat cgactgcaag agtggcaaag cccacatcgc cctgttccat
    26641 cattcctgcc actgcgggca gcaacaccag cgtcagaacc atcaccaggt cttccacaat
    26701 caaccaaccg atggcgattt tcccacgctg actgtctatt aattgccgtt cttcaagtgc
    26761 gcgcagtaac accacggtac tggcggtgga aagacataag ccgaacacga taccggtcat
    26821 taacgaccag cccagcacgg cggagagcgc catacccagc agcgtcgcca cggctatctg
    26881 ggcgatcgcg ccgggaatgg cgatggactt taccgccatc aaatccttga gcgaaaaatg
    26941 caaaccgacg ccgaacatca gcaaaataac gcccagttca gccagttccg gcgcaagctt
    27001 ggtatcggca acaaagcccg gagtgaatgg tcctgccagc acacccgcta acagatatcc
    27061 caccagagga gaaatacgta gtttattggc cagcatgccg aggataaagg cgagcacaag
    27121 gccgccaaca atggtggtga taagcggggt ggcgtgatgc attccgtctc cttttcctgg
    27181 tggttattgt ccatttttgg ccgggaaaac caaaattaca ggtaatagtt tatgacaatt
    27241 tcattgatga tgtttatgaa taattgttga attttgcaga aaaatggaat tagctgcaaa
    27301 aaaagcacgg atcggaaaac agaaggcggt ttaacagagg aaaggcttat ggcgactgta
    27361 aaacgatgca gccaaagttt gcctttggct gcaatgaaat cagtctttat gccggttatc
    27421 aggcaggaat atggtcaaca tccccaatag tggcaggaaa gcacagattt tatagactaa
    27481 ctcgatgctg gtgtgatcgg cgataagccc cagaactgcc gctcccagac ctcccatgcc
    27541 gaaagcaaaa ccgaaaaaga gtccagaaat cataccgata cgtcccggaa gtagctcctg
    27601 agcgtagacc agaatggcag agaatgccga agcgaggata aatccaataa tcaccgttaa
    27661 aacccccgtc cagtgcaggc gggcgtaggg taaaatcagc gtaaacggcg caacgccgag
    27721 gatagagccc caaatcacat atttccgtcc aattttatcc cctacaggcc cgccgatcac
    27781 cgtacctgcc gcaacggcaa acaggaaggc aaacagatga agctgggcat tctggataga
    27841 taatccgaat ttttgcatca gataaaaggt gtaatagctg ctgatgctcg ccatatagaa
    27901 atatttcgag aaaatgagga ttaacagaat gctgactgcc agtaccactt tgttgcgcgg
    27961 cagtggattg attatcgtcg ctttgggttt tcctttattc attcggtgct gtgccgagta
    28021 ccaacggcta atttgcgcca acaccacgat cgccagcagt gccgcaagca caaaccaggc
    28081 aacgttgcct ttgccataag gcgcgataat caccgccgcc agcaaggggc cctgggaact
    28141 gccaaagttg ccgccgacct gaaagataga ttgcgccagg ccatgccgcc cgccggaagc
    28201 catacgggct acgcgagaag attccggatg aaagaccgat gaaccggtac cgaccagcgc
    28261 cgccgccagc agaactgcgc caaaactgcc cgccagcgca agcagcacca gaccacttaa
    28321 ggtaaagcac atgccaattg gcaacgacca cggcatcgga tatttatcgg tccagtagcc
    28381 gaccactggt tgcagtagcg aagaggcgag ctggaaggtg agggttatca tgccaatctg
    28441 cataaatgtc agagaaaatt ctgactgaag cagcggataa atcgccagaa tcagcgattg
    28501 gatcatgtcg ttcagcagat gtgagaggct gatagcacct aaaataccaa acgatgttcg
    28561 ggccttggtc gttgacgcag ccgcgcccgc cacaggctgg gtttgttcac tcattgccat
    28621 aggaaagtca ctttttcagg gttgcgatgt aaagaatgat cttatttgtg attattaccc
    28681 gactaacata cctgtatgcg tcgtctgaag gaagtctcaa cgccgaatac agaatttcta
    28741 atctggatgc agatttatct tcaccggacg cagacttgtc tatgatgtcg cgtcatacta
    28801 ttttttaaca cgttgaaatc aggtcaggga gagaagtatg aaattattgc agcggggcgt
    28861 ggcgttagcg ctgttaacca catttacact ggcgagtgaa actgctctgg cgtatgagca
    28921 ggataaaacc tacaaaatta cagttctgca taccaatgat catcatgggc atttttggcg
    28981 caatgaatat ggcgaatatg gtctggcggc gcaaaaaacg ctggtggatg gtatccgcaa
    29041 agaggttgcg gctgaaggcg gtagcgtgct gctactttcc ggtggcgaca ttaacactgg
    29101 cgtgcccgag tctgacttac aggatgccga acctgatttt cgcggtatga atctggtggg
    29161 ctatgacgcg atggcgatcg gtaatcatga atttgataat ccgctcaccg tattacgcca
    29221 gcaggaaaag tgggccaagt tcccgttgct ttccgcgaat atctaccaga aaagtactgg
    29281 cgagcgcctg tttaaaccgt gggcgctgtt taagcgtcag gatctgaaaa ttgccgttat
    29341 tgggctgaca accgatgaca cagcaaaaat tggtaacccg gaatacttca ctgatatcga
    29401 atttcgtaag cccgccgatg aagcgaagct ggtgattcag gagctgcaac agacagaaaa
    29461 gccagacatt attatcgcgg cgacccatat ggggcattac gataatggtg agcacggctc
    29521 taacgcaccg ggcgatgtgg agatggcacg cgcgctgcct gccggatcgc tggcgatgat
    29581 cgtcggtggt cactcgcaag atccggtctg catggcggca gaaaacaaaa aacaggtcga
    29641 ttacgtgccg ggtacgccat gcaaaccaga tcaacaaaac ggcatctgga ttgtgcaggc
    29701 gcatgagtgg ggcaaatacg tgggacgggc tgattttgag tttcgtaatg gcgaaatgaa
    29761 aatggttaac taccagctga ttccggtgaa cctgaagaag aaagtgacct gggaagacgg
    29821 gaaaagcgag cgcgtgcttt acactcctga aatcgctgaa aaccagcaaa tgatctcgct
    29881 gttatcaccg ttccagaaca aaggcaaagc gcagctggaa gtgaaaatag gcaaaaccaa
    29941 tggtcgtctg gaaggcgatc gtgacaaagt gcgttttgta cagaccaata tggggcggtt
    30001 gattctggca gcccaaatgg atcgcactgg tgccgacttt gcggtgatga gcggaggcgg
    30061 aattcgtgat tctatcgaag caggcgatat cagctataaa aacgtgctga aagtgcagcc
    30121 attcggcaat gtggtggtgt atgccgacat gaccggtaaa gaggtgattg attacctgac
    30181 cgccgtcgcg cagatgaagc cagattcagg tgcctacccg caatttgcca acgttagctt
    30241 tgtggcgaaa gacggcaaac tgaacgacct taaaatcaaa ggcgaaccgg tcgatccggc
    30301 gaaaacttac cgtatggcga cattaaactt caatgccacc ggcggtgatg gatatccgcg
    30361 ccttgataac aaaccgggct atgtgaatac cggctttatt gatgccgaag tgctgaaagc
    30421 gtatatccag aaaagctcgc cgctggatgt gagtgtttat gaaccgaaag gtgaggtgag
    30481 ctggcagtaa tccgaaagtg ccggatgttt gcatccggca caatgcttaa tcgcggcggg
    30541 cgatatcagc aaatttggca tcgaggatct ttgccagatc gcctgccgcc agttcgatat
    30601 ccagtccgcg cttgccgccg gaaacataaa tagtggcaaa ttcttgtgcg ggggcgtcga
    30661 taatcgtggg cagacgtttt ttctgcccca gtgggctaat ccccccaacc aggtatcccg
    30721 tcgaacgctg cgcgaccatc ggatcggcca tctcaacttt cttggcaccc agcgcttttg
    30781 ctactttttt aagatccagt tgacctgcga ccggcgtaac ggccacggca aggtgtttca
    30841 tatcaccgtt cactgccacc agcagcgttt tgtagacctg atccggattc aaacctaatt
    30901 ttttgacgac ttcatcgcca aaattggttt cagccggatc gtgctcgtag gtatggattt
    30961 gaaacgaaat cttgtttttt tcgagtaatt taactgcggg tgtcatggta attttcctgc
    31021 cttaaacaaa aaaacgaagc aagcatacgc ctgatggcgg tccaaaaaaa agagtcatct
    31081 tgcctaagag tattggcagg atggtgagat tgagcgacaa tcgagttaca ccgtcgctta
    31141 aagtgacggc ataataataa aaaaatgaaa ttcctctttg acgggccaat agcgatattg
    31201 gccatttttt tagcgcaaca tttgcggcaa attcccttct ccatacaggt gtagtgcacc
    31261 gaccgcgacc acatatcgcc ccggcggcat ggcgcgtaat ttatcccgcc aggcgagatt
    31321 tcgctgatgc atcagcacat cgtacagcga ctgactgaac gtattgggca gcgttatatc
    31381 attattttgc ggcggtgcat tcagccacca gctcatcatt tgttgcagca accgtgcgtt
    31441 ggtatgccag tgggtcagcg tatcgtccag cagcgccagt cctttgtcag ggagctggag
    31501 caacatggca atctggtttt cagccccttc cagttcaatc acgggtttat gttgttgctt
    31561 cgccgcctgc aatagctggt aatcgatacc gtattctgcc cgcagcccca gtttttgtgc
    31621 ctgcgtcgcc tgaagaacca tcgcgatttg ccacagcggt tgggtagaaa agagtgaggg
    31681 agaaatgccc atctcctggc taatgtgctg caggttttgg agttgctcct cgctaatgcg
    31741 ctcttccagc gcctcgcagg caggcaaatt agcaaaaggc gtatcgctgg tggaaacatc
    31801 cgcctcgacg atcagcgcat cggcgttttt gagctttttg agcaaacggg tgggcagggg
    31861 agccatatcg tggctaccca tatgaatact gccaatcaga tgaaaatggc gattgccggg
    31921 gagggtgata tcgatggcag gccaggtgta atgattaccg cgcagcgcgg cccaaagcgt
    31981 ttttacccgg tacaacagat ccatacgacc tccctttgtg aaatatcatg ctagcgcgcg
    32041 gtgagggatg gcgcaaatgg agttggggac ttgtttaatg tgtttgtatg attcagtatg
    32101 ttcttgcatc gctattcaca gggaagcaac agttaaaaac catgaaacag gcaacaagaa
    32161 aaccgactac acccggagat attcttctct atgaatatct ggaaccgctc gatttgaaaa
    32221 tcaatgagtt agcagagttg ctgcatgttc atcgtaatcg cgtcagtgca ctgatcaata
    32281 acaatcgtaa actcaccact gagatggcat tccgtctggc gaaagttttt gataccacag
    32341 tcgattttag gctaaacctc caggcggcag ttgatctttg ggaagttgaa aacaacatgc
    32401 gcacccagga agaattggga cggattgaaa cagtggctga atatttggca cgccgtgaag
    32461 agcgtgcaaa aaaggtcgcg taaggccaaa acatcgcttc atctcgtagg ccggataagg
    32521 cgttcacgcc gcatccggca tccgagcacc attgcctgat gcgacgctta cgcgtcttat
    32581 caggcctaca aacctgtgcc tgaaccgtag gccggataag gcgtttacgc cgcatccggc
    32641 agtcatgcgt cgatgccaaa tgcgccgccc taaagcagcg catccgcaat gacgtactta
    32701 ttccttcggt ttaaaccgca gcaaccggtt ggcgttactc actacggtaa tcgacgagag
    32761 cgccattgcc gctccggcaa ctaccgggtt aagcagtgtt ccagtaaacg gccacaaaat
    32821 accggcggcg accggaatac cgatgctgtt gtagataaag gcaccgagca ggttctgctt
    32881 catgttgcgc agcgttgcgc gggaaatcgc gagcgcatcc gcaacgccca tcaggctatg
    32941 gcgcatcagg gtaatcgccg cggtttcaat ggcaacatca ctgccgccac ccatcgcaat
    33001 gccgacatcc gcctgggcca gcgctggcgc gtcgttaatg ccgtcgccca ccattgccac
    33061 ctgacgtcct tcactttgca gacgtttgat cgcttcggct ttaccgtccg gcagcactcc
    33121 ggcaatcact tcatcaattc cagcttcttt ggcgatcgca ttggcggtgg ttgggttatc
    33181 cccggtcaac atcaccagac gatatcccgc tttatgcagg cgttgcagcg ccgccacgct
    33241 atcactacgc aacggatcgc gtacagccag cagggctact gctttcccgt caatcgccag
    33301 cagcacaggc gttgctcctt gcgatgcctg agctgaaata tcggcttcaa tcgctttagt
    33361 atcgacttgc tgatcattta acaacgcctg attacccagc aataacgcat gaccttctgc
    33421 ttcaccgctc acgcccagcc cgcgcaatgt gcggaaaccg ttgacctgcg gtagctgcat
    33481 atcgcctgct ttatcaagga tcgctcgtgc caatggatgg ctggaacctt gctccagcgc
    33541 cgccgccaga cgcagcgcct gcgcttcgtc aacatcagca aatgttttca ctgcgacaac
    33601 ctgcggcttc ccttcggtca gcgtcccggt tttatcgaac acgacggtat cgagtgtact
    33661 ggcgcgttgc agcgcgtcgg cgtcccgcac cagcacgcca aactcagccg cccgcccgac
    33721 gccggaaata atcgacatcg gcgtcgccag tcccagcgca cacgggcagg cgataatcag
    33781 caccgtagtg gcaatcacca gggtatagac aatctgcggt gccggaccaa agaaatacca
    33841 gattgccgca ctgacaagcg caataaccac tactaccggc acaaatacgg ctgagatttt
    33901 atccgccagc tgaccgattt ctggcttgct gctctgggcc tggcgcacca tgcgaatgat
    33961 ccttgatagc gtggtatggc tgccaaccgc actggcacga aacagcacac tgccgtcctg
    34021 taccactgtc ccggcatgga cgctatcgcc ttcgcctttt tgctgcggga ttggttcgcc
    34081 cgtcagcatc gcttcatcca gccatgcttc gccctgggta atctcgccat ctaccggcac
    34141 gcgatcgccg gtcgtcaggc gcagcaacat acctggctgc acttctgcca gaggcacgct
    34201 tttttcacct tcgtcagtaa ccaggcgcgc cgtcggtggg gttaaatcga gtaatttctc
    34261 cagtgcctta gaagaacgct gacgtgcgcg tgcttccagc atatggccga gattgatcag
    34321 accgataatc atcgcactgg cttcatagta aagatgtcgc gcttccatcg ggaaccactg
    34381 cggccacagg ttgacgctca tcgaatagag ccacgccacg cccgttccca gcgccaccag
    34441 cgtatccatc gtcgctgcac cgttcagcag gcttttccat gcactgcggt aaaaatggcc
    34501 gccggcgaaa accatcaccg ccagggttat caggccgata accaaccaca ggctgcggtt
    34561 gtcggcggtg accatcatgt tatcgccgat catcccccag accatcaccg ggatacccac
    34621 cgccagtgcg acaattgcct gccagcggaa gcgcttcatc gtagcgacgg cggtttcttg
    34681 ctggcgttcg cggcgtttcg cgtcatcttc aatcgcttcc gcgccgtagc ccgctttttc
    34741 caccgcctgc actaaatctt gtggggaggc actgcccatc accagcgcag tacgctccgc
    34801 caggtttacc cgtgcctgag tgacgcccgg tacgctttgc agcgcatttt gtacgcgggt
    34861 gacacagctg gcgcagctca tgccgctcag cagcaactgc tggctgtcat cgtcatcggc
    34921 ggtcgctgcc ggaagcgcct cagaaaccgc tgtcagtgct tccgacggga ttgatgactc
    34981 cgccagcggt ttagcctttg ggtggcttac agatgcgtca taacccgctt gtttgatggt
    35041 ttcgatcagc tgttctgcac tggcagtccc ggtaacgtgc gcttcagtga tagacacatc
    35101 cgcttgttcc acatccgggc gctgttcaag actttctttc acgcgtttaa cgcagtgacc
    35161 gcaggacagg ccgtccaggt tcaggtcgat agtttgtgac ataaaacact cctttaagac
    35221 agttttgact ggctgtgata aaggttaaac cttccagaaa ggggaaggtc aagaaattaa
    35281 taaaccaggc gggtaaaagt ccgtaaagat taaaaaatcg gctcgatttg catcaggatt
    35341 agacatttat ttctttgttt tcctgtagtt aagttgcggg tgctaagtta aatcaggatg
    35401 cctgaaaatc ggcacagggg tgaggaatta cctcccgcat ctataaaaag gagttaacaa
    35461 aagatgttag atgcaaacaa attacagcag gcagtggatc aggcttacac ccaatttcac
    35521 tcacttaacg gcggacaaaa tgccgattac attccctttc tggcgaatgt accaggtcaa
    35581 ctggcggcag tggctatcgt gacctgcgat ggcaacgtct atagtgcggg tgacagtgat
    35641 taccgctttg cactggaatc catctctaaa gtctgtacgt tagcccttgc gttagaagat
    35701 gtcggcccgc aggcggtaca ggacaaaatt ggcgctgacc cgaccggatt gccctttaac
    35761 tcagttatcg ccttagagtt gcatggcggc aaaccgcttt cgccactggt aaatgctggc
    35821 gctattgcca ccaccagcct gattaacgct gaaaacgccg aacaacgctg gcagcgaatt
    35881 ttacatatcc aacagcaact ggctggtgag caggtagcgc tctctgacga agtcaaccag
    35941 tcggaacaaa caaccaactt ccataaccgg gccattgcct ggctgctgta ctccgccgga
    36001 tatctctatt gtgatgcaat ggaagcctgt gacgtgtaca cccgtcagtg ctctacgctc
    36061 atcaatactg ttgaactggc aacgcttggc gcgacgctgg cggcgggtgg tgtgaatccg
    36121 ttgacgcata aacgcgttct tcaggccgac aacgtgccgt acattctggc cgaaatgatg
    36181 atggaagggc tgtatggtcg ctccggtgac tgggcgtatc gcgttggttt accgggcaaa
    36241 agcggtgtag gtggcggtat tctggcggtc gtccctggcg tgatgggaat tgccgcgttc
    36301 tcaccaccgc tggacgaaga aggcaatagt gttcgcggtc aaaaaatggt ggcatcggtc
    36361 gctaagcaac tcggctataa cgtgtttaag ggctgatcat gatgaacacg gaaggtaata
    36421 acggtaacaa acccctcggt ctatggaacg tcgtttccat cggtattggg gcaatggtgg
    36481 gggcggggat cttcgcgctg ctggggcagg ctgcgttgct aatggaagcc tcgacctggg
    36541 gcgcctttgc ttttggcggt attgtggcga tgttttccgg ttatgcctat gcgcgtctgg
    36601 gggcgagcta tcccagcaat ggcggtatta tcgacttttt tcgtcgcgga ttaggcaacg
    36661 gcgtcttttc gctagcgctc tcgttgctat acctgttgac gctggcggtg agcatcgcca
    36721 tggttgcccg tgcttttggc gcttatgccg tgcagttttt gcatgaaggc agccaggagg
    36781 agcaccttat tttgctctac gcgttgggga tcattgcggt gatgacgctt ttcaactcct
    36841 taagcaacca tgcggtaggg cggctggaag tgatcctcgt cggcattaaa atgatgatcc
    36901 tgttattgct gattattgcc ggtgtctggt cgctgcaacc ggcgcatatt tccgtctctg
    36961 cgccccccag ctccggtgcg ttcttctcct gtattgggat aaccttcctt gcctatgcgg
    37021 gctttggcat gatggcgaac gcggcggata aagtgaaaga tccgcaggtc attatgccac
    37081 gggcgtttct ggtggcgatt ggcgttacca cgttgcttta tatctcgctg gcgctggttt
    37141 tgcttagcga tgtatcggca ttagagttag aaaaatatgc tgataccgcc gtagcgcagg
    37201 ctgcttctcc gctgctcggg catgtgggtt atgtgatcgt cgtcatcggc gctttactgg
    37261 cgacggcttc aggcattaac gcgaacctgt tcgccgtgtt taacatcatg gacaacatgg
    37321 gcagcgaacg cgaactgccg aagctaatga ataaatccct gtggcggcag agtacctggg
    37381 gcaacatcat cgtcgtggtg ttgataatgc tgatgacggc ggcgctaaat ttagactcac
    37441 tcgccagcgt tgccagcgcc acctttttga tctgctatct ggcggtgttt gtggtggcga
    37501 tccgcctgcg tcatgatatt cacgcctcgt tgccgattct tatcgttggt acgttggtga
    37561 tgttgttggt gatcgttggc tttatctaca gcctgtggtc ccagggcagc cgtgcgttga
    37621 tatggattat tggtgcaatc ttactcagcc ttattgtggc aatggtcatg aagcgtaata
    37681 aaaccgtata acacctctct gtgcgcagta cttcctgtat tattgtggtg gcggtcgata
    37741 ttcgcactgg caaaaaaacg tgcttgaata tctgttgaaa ccctttaaca aagcacagga
    37801 ggcgttgcgc gaacgatgaa catcagcgat gtagcaaaaa ttaccggcct gaccagcaaa
    37861 gccattcgct tctatgaaga gaaggggctg gtgacgccgc cgatgcgcag cgaaaacggt
    37921 tatcgcacct acacgcagca gcatctcaac gaactgacct tactgcgcca ggcacggcag
    37981 gtgggcttta acctggaaga gagcggcgag ctggtgaatc tgtttaacga cccgcagcgg
    38041 cacagcgccg acgtcaaacg gcgcacgctg gagaaggtgg cggagatcga acgacacatt
    38101 gaggagctgc aatccatgcg cgaccagctg ctggcactgg cgaatgcctg ccctggcgat
    38161 gacagcgccg actgcccgat tatcgaaaat ctctccggct gctgtcatca tcgggcaggg
    38221 tgattaagac gagacggctc ggatatgtag ggttatccct tctatcgcaa tgacttcaac
    38281 atacgtacct gcaccgagat cctcgctggc gctgacaggc catgaactgt caccgacgcg
    38341 catatgaccg cgcccgttga ccagcggaga ttccagcaca aaacgtcggc caatcagctg
    38401 ctgcccgcgc tggtttaaat gactatcgct gtgcttttgt tcgcgcaccc gccgcgacaa
    38461 ccatttccac cacagccagg cggcgagcag cgtcaggatg gcaaacatta ccccttgcca
    38521 ctcccaaccc agcggcacca gccagaccac caggccggta atcaccgctg ccacgccgct
    38581 ccacaacaaa taaccatttc cgcccagcat ctcggctgcc agcagcaacc cgccgagact
    38641 gagccagaaa atatgtggat gaacgaccat taactccatc atggctgagt ccgtttgttg
    38701 gcgctgtctt tcaccagctc ggcaatcccg gcaatcgacc ccatcaggct gctggcctct
    38761 aatggcatca tcactacttt gctgttactg gaggaaccga tctgctgtaa cgcttcggtg
    38821 tatttttgcg ctacgaagta gttcaccgcc tgaatatcac cggaggcgat agcttcagac
    38881 accattttgg tggcgcgggc ttctgcttcg gcggaacgtt cacgcgcttc agcctgtaaa
    38941 aacgccgact gacgttcgcc ttccgctttc aggatttgcg actgtttttc accttcggct
    39001 ttgaggattt ccgcctgacg gatcccttcc gcttcaagaa tgtaagcgcg tttggtacgt
    39061 tccgctttca tctgcgcgtt cattgaagag ataagctctg ccggtgggcg cacgtcgcga
    39121 atttcaatac gggtgacttt aatcccccac gggttggtgg cttcatcgac aatacgcagc
    39181 aggcgtgagt tgatgctgtc gcgctgagag agcatttcgt caagttccat tgaacccaac
    39241 acggtacgga tgttagtcat ggtcaggttg atgatcgcca gctccagatt gctgacttca
    39301 taggcagcgc gtggcgcgtc aatcacctga ataaaacaga ctgcgtcgat ggtaacgttg
    39361 gcgttatctt tcgagataac ttcctgggaa gggatatcga gcacttgctc catcatattg
    39421 atcttgcgac caatgcgatc cataaacggc accaccagac tgagccccgg ctgtaacgtt
    39481 ttggtatagc gaccaaaacg ttctaccgtc cattgatagc cctgcggcac gattttgacg
    39541 cccgcgccga caatgaccag cgcgacaaaa atgagaatcg ggataaagat aagcatcgga
    39601 aaaacctcct gttgtaccgt ccataatcag caaaattgct gcttgattaa acaaattata
    39661 cctgattact gaaagagagt tcccccttat tcctgcgaag gataaactgt ttttagtaaa
    39721 aatcagaaaa agggaacagc gatgcaggaa aatagtcctt tgcttcagct acaaaacgta
    39781 ggatatctgg cgggtgatac gaagattctt aataacatca atttttcgct gcgtgctggc
    39841 gaatttaagt taattaccgg tccttctggt tgtggcaaaa gtacgctgct aaaaatagtt
    39901 gcttcattga tcagcccaac cagcggaacg ttactgtttg aaggtgagga tgtcagcaca
    39961 ctaaaaccag aaatctaccg ccagcaagtc tcttactgcg cccagacgcc gacgctgttt
    40021 ggcgatacgg tatacgataa tctgatcttt ccctggcaga tccgtgacca gcagcctgac
    40081 ccggccattt ttctcgattt tctcgaacgc ttcgctttgc cggacagcat tttgacgaag
    40141 aatatcgccg agctatctgg tggtgaaaaa caacgcatct cattgattcg taacctacaa
    40201 tttatgccga aggttttatt gctggatgaa ataaccagtg cgctggatga aagtaataaa
    40261 cataacgtca atgagatgat ccatcgttat gtgcgcgagc aaaatattgc cgtgctgtgg
    40321 gtgacacacg ataaagacga aattaatcat gcggataaag tgattacact gcaaccgcat
    40381 gccggagaaa tgcaggaagc acgctatgaa ctcgcataat attactaacg aatcattagc
    40441 actggcatta atgctggtgg tggtggcaat cttaattagc cataaagaaa aactggcgct
    40501 ggagaaagat attctctgga gcgtcgggcg agcgataatt cagctgatta ttgtcggcta
    40561 tgtgctgaag tatattttca gcgtggatga tgccagcctg acattattga tggtgttatt
    40621 tatctgcttt aatgcggcgt ggaacgcgca aaaacgcagt aaatatattg ctaaagcttt
    40681 tatctcatcg tttattgcca ttacggtcgg ggcgggaatt accctggcgg tgctgattct
    40741 ctcagggtcg attgaattta tcccgatgca ggtgatccct gtcgccggga tgattgccgg
    40801 taacgccatg gtagcggtgg ggttgtgtta caacaattta gggcaacggg tcattagcga
    40861 acagcaacag attcaggaga aactgagtct tggcgcgacg ccgaagcagg cttcagcgat
    40921 attgattcgc gacagtattc gcgcggcttt aattccgacg gtcgattcag caaaaacggt
    40981 tggcttagtg agtttaccag gaatgatgtc cgggctgata tttgccggga ttgatccggt
    41041 gaaggcgatt aaatatcaga ttatggtgac ctttatgctg ctctcaaccg ccagcttgtc
    41101 gaccattatt gcctgctatt taacctatcg taagttttat aattcgcgcc accagttggt
    41161 ggtgactcaa ttgaagaaga aatgatgcga tgccggatgc gcagcatcgc atccggcgtt
    41221 gtggttcatg tgccgaacaa cggaatatca atacaacaat gcatacagct ggcggcgata
    41281 cttcgacgcc agtgcatcac ccgtacccag cgcagcgagg atctcctgga acgttttacg
    41341 cgtctgaccg tctgcggcgg tgagatcttt acgcagatgc ccgaacagca actccagcgc
    41401 ctcttcattg cgcccaacct gatgcagttg cagcgccagt tgcgtcgcca gtgcggcatc
    41461 ttctggattc tccgccacct gctgttgcaa ctgttgaatt tccggcttat cagccgcctg
    41521 cttcagcagt tcgatttgcg ctaccagccc ctggtagcgg gtgtcctgat cctgcaacgg
    41581 aatggttttc agcaccgctt ccgcatcttc agaacggttc agcgcaatca gcgtttctgc
    41641 cagcagcagg ccgatctccc cgttctgatt cgacaactgc caggcgtctt tcagcaatgg
    41701 cagggcatcg gtgtaattgc cttcctgcat cagttgcatc gcctgctgcg ctttcagctc
    41761 ttcttcgcgc ggcagcactt tatccagcag ggcgcggatc gcctcttccg gttgcggccc
    41821 ctggaagcca tctaccggtt gcccgttctg gaacagatac acggtcggaa tcgcgcgcag
    41881 accaaactgg gcggcaatca tctgctccgc gtcgcagtcc agcttcgcca gaataaattg
    41941 cccgttgtac tgcgccgcga ggctttccag gatcggggtt aactgcaaac agtgctggct
    42001 acgttcagac caaaaataga acagcaccgg agtggtcatc gactgttcaa gaacctgttg
    42061 caggttagat tcgttaatgt tgacaatatt ttctacggac atggagtcgc tctctgttgt
    42121 cgatattttc tttgacatgg gggcttaagc gcgcgcttca actcacccct gcaatatttt
    42181 gtccatcacg cgcccaggca gcaggcgctc gagcaccatt actgcccagg tcaccagcgt
    42241 taccggatag cgcatcttcg gcttctcgct aataaaagca tggcgcactt tgtcgaccac
    42301 cgcttccggt cccaacgtaa agcgggcggc gatgccggga ttttcgactg gtttatcact
    42361 ttgcgtctgg ttgacgttgt cggtgaagcg agtacgaatg ggaccgggtt cgatcaggct
    42421 gactttaatt ccgctgtggc gtagctccat gcgcagcgcg tctgaccacg cctccagcgc
    42481 atatttactg gccgcgtaag cgccacgacc cggcgtggag attaatccca tcaccgatga
    42541 tgtcatcaca atacgccctt caccgtgcgg taacatcgcg ggtaacaggc gcatggtgag
    42601 ctggtgtgcg ccgaaaaagt tggcggaaaa ctgctgttcc atctgcgcac ggctgatggt
    42661 ggaaaggggg ccatacatgc cgaatccggc attgttaaag atcccataca gacaattatc
    42721 ggtcagggcg atcacctcgt ctgctgcgcg attaacactt tctggtgaat ccagatcgat
    42781 caacacgccg gtaaatccca tgttgttcat acgctcaaca tcattcggtt ttcggcaacc
    42841 tgccagcaca tgaaaaccct ggcgttttaa ttcgagcgcg ctttccaggc caattccact
    42901 ggaacatccg gtaattaaga ccgatttttg cataacttta cctgtcagga tctccgttgc
    42961 tttatgagtc atgatttact aaaggctgca actgcttcgc catccagtcg gcaataaacg
    43021 gctgggcgtc gcggttggga tgaataccgt catcctgcat ccattgtggc ttgaggtaga
    43081 cctcttccat aaaaaagggc agcagcggaa catcaaactc tttggcgagt ttggggtaaa
    43141 tggcgctaaa ggcttcatta taacggcgac catagtttgc aggcagacgt atttgcatta
    43201 acaatggttc agcgttggcg gctttgacat cctgcaaaat ctggcgcagc gtttgctcgg
    43261 tttgctgtgg ctgaaaacca cgcaaaccat cattgcgccc agttcaacca gcacccaacg
    43321 cggctgatgc tgtttgagca gagccggaag gcgcgccagc ccttgttgcg aggtgtcgcc
    43381 gctgatgctg gcattaacta ccgacgtttt actctgccac ttatcattca acaaggcagg
    43441 ccaggccgcg ctggcagaca ttcgatatcc ggcgctcagg ctatcaccca gaatcaataa
    43501 cgtgtccgct gcggcggcac ggaaggttaa caggaccagg aacaggaagg gcaaatgcca
    43561 gcggaaaaca ttgttgaagt tcatcatctt aagaagtccg tcggtcaggg ggagcatgaa
    43621 ctctccatcc tcaccggagt tgagctggtt gtcaaacgtg gcgagaccat cgcactggtg
    43681 ggcgagtcgg gatcgggtaa gtcaaccttg ctggcgatcc tcgccgggct tgatgacggc
    43741 agcagtggcg aagtgagtct ggtgggacaa ccgctacata atatggacga agaagcgcgg
    43801 gcaaagttgc gcgcgaagca cgtcggcttt gtttttcagt catttatgtt aattcctacc
    43861 cttaacgcgc tggaaaacgt cgagcttccg gctctgctgc gcggtgagag tagcgcggaa
    43921 agtcgtaacg gggcgaaagc gttgctcgaa cagttagggc tgggtaaacg tctggatcat
    43981 cttccggcac agctttccgg cggtgaacag caacgagtgg cgctggcacg agcctttaat
    44041 ggtcgacctg atgtgctgtt tgccgacgaa cccaccggca accttgaccg ccagacgggc
    44101 gataaaattg ccgacctgct gttttccctc aaccgtgaac atggcaccac gttgattatg
    44161 gtgacccacg acctgcaact ggcggcacgc tgcgaccgct gcttacggct ggtgaacggg
    44221 cagttgcagg aggaagcatg attgcacgtt ggttctggcg cgaatggcgt tcgccgtcgc
    44281 tattaattgt ctggctggcg ctaagcctgg cggtggcctg cgtgctggcg ctgggcaata
    44341 tcagcgatcg catggagaag ggcttaagcc agcaaagccg tgagtttatg gcgggcgatc
    44401 gggcgttgcg cagttcacgc gaagtgccgc aagcgtggct ggaggaagcg caaaagcgcg
    44461 gcctgaaagt cggcaagcag ctgactttcg ccacaatgac ctttgcaggc gacacaccgc
    44521 agctggcgaa cgtcaaagcg gtggatgata tctacccgat gtatggcgat ctgcaaacta
    44581 atccccctgg cctgaaaccg caggcgggca gcgtattgct ggccccacgc ctgatggcac
    44641 tgcttaacct gaaaacggac gacaccattg acgtgggcga tgccaccttg cggattgccg
    44701 gagaagtgat tcaggaaccg gattccggtt ttaacccctt ccagatggct ccgcgtctga
    44761 tgatgaatct ggcggatgtc gataaaaccg gagccgtgca gccggggagt cgggtcacct
    44821 ggcgttataa attcggcggc aacgagaacc agctcgacgg ctatgagaaa tggttgttac
    44881 ctcagcttaa acccgaacaa cgctggtacg gtctggaaca ggacgaaggc gcgctggggc
    44941 gatcgatgga acgctcgcaa cagttcctgc tgctttcggc gcttctgacc ttgctgctgg
    45001 cagtggcagc ggtggcggta gcgatgaatc attactgtcg cagtcgctac gatctggtgg
    45061 cgatcctcaa aacgctgggg gcagggcgag cgcaactgcg taagctaatc gtcggtcagt
    45121 ggttgatggt gctgacgctt tcagccgtta ccggtggggc cataggcctg ttgttcgaaa
    45181 acgtgttgat ggtgctgctc aagcccgttc tgcctgctgc actaccgcca gccagcctct
    45241 ggccgtggct gtgggcgctt ggcaccatga cggtcatctc gctgctggtg gggctacgac
    45301 cataccgctt gttgctggca acgcagcctt tacgcgtatt acgtaatgat gtggtcgcga
    45361 acgtctggcc gctgaagttt tatctgccga ttgtcagtgt ggtggttgtg ctgctgctcg
    45421 ccggattaat gggtggcagc atgctgcttt gggcggtgct ggcgggcgcg gtagtactgg
    45481 ctttgctgtg cggtgtgctg ggctggatgc tgctgaatgt acttcgccgc atgacgctga
    45541 aatcgctgcc tctgcgcctg gcggttagcc gcctgttacg tcagccgtgg tcaacgttaa
    45601 gccagctttc ggcattttcg ctctccttta tgctgctggc actgctgctg gtgctgcgtg
    45661 gcgatctgct cgaccgctgg cagcagcagc taccgccaga aagcccgaac tactttttaa
    45721 ttaacatcgc cacagaacag gtagcaccgc taaaagcgtt cctcgcggaa catcatatag
    45781 tcccggaatc attttatccg gtagtgcggg cgcggctgac ggcgattaac gataagccga
    45841 cagaaggtaa tgaagatgag gcgcttaatc gcgaactcaa tcttacctgg caaaatacgc
    45901 ggcccgatca taatccgatt gtcgccggta actggccgcc aaaagccgat gaagtgtcga
    45961 tggaagaggg gctggcgaaa cgcttaaacg ttgccctcgg tgataccgtg acttttatgg
    46021 gcgataccca ggagttccgc gctaaagtga ccagcctgcg caaagtggac tgggaaagtc
    46081 tgcggcctaa tttctatttt attttccctg aaggggcatt agacgggcaa ccgcagagct
    46141 ggcttaccag tttccgctgg gagaatggca acggcatgtt gacgcaactc aaccgccagt
    46201 tcccgaccat tagcctgtta gacattggcg cgattttaaa acaggtcggt caggtgctgg
    46261 agcaggtaag tcgggcgctg gaggtgatgg tggtgctggt caccgcctgc ggtatgttgc
    46321 tgttgctggc acaggtgcag gtgggaatgc gccagcgtca tcaggagctg gtggtatggc
    46381 gcacactcgg tgcggggaaa aaactgctgc gcacgacatt gtggtgtgag ttcgccatgc
    46441 ttgggtttgt ttccggcctg gtggccgcaa ttggtgcgga aacggcactg gcagtgttgc
    46501 aggcgaaagt gtttgatttc ccgtgggagc cagactggcg attgtggatt gttctgccgt
    46561 gcagcggtgc gctgctgctg tcgcttttcg gcggctggct gggtgcgcga ctggttaagg
    46621 gtaaggcgct gttcaggcag tttgtggggt gatgacaggt gttagattgc ctgatgcgct
    46681 acgcttatca ggcctacgaa gagcgtgcaa tatattgatt ttgcaagatt gtgtaggccg
    46741 gataaggcgt ttacgccgca tctggcatga acagtgtgca ttttggcaat aaatatgaaa
    46801 ccaactgtca tgtcggtttt tttaaaggcc gaaggaggta aaccttcggc cttagctacg
    46861 tttcctcagt tattcaataa actgacggat cagttccagc agcgcgatta gcgccttaag
    46921 aacgatgaga actttatcga gaaaagtttt catccgttgc tcccttaagg gagaaagaca
    46981 atcacagctt acccttacgt tgcctgtaac tatgattttc ttcatgtgct agatgaggac
    47041 tgcctctctc caaattagag caccagaatc agcaccacct gtatcctgac caggacttaa
    47101 aagtactttt ccgcactgcg gtaatccctt gaggcagtat cactactagc gtcgggattg
    47161 tatcgccaac atagctgttt gaatatgtag gcatcctgac ttataattca cttgctagat
    47221 gaggactgtt taatttctgc ccggaacaac caagtcttgg gcggaaaaaa taaaaaaacc
    47281 gactgtcatg tcggtttttt atatctgtat tctggtgcaa ccattttgtt ctcacgacaa
    47341 tcgccgtttc agaataccgc ccatcactgc gccttaaccc ataccgccac ttccgcccac
    47401 tcaccgcgaa agacaacttt ttccgctttt ttctcaagct gatagcgata cattgggtcg
    47461 taatattctt taagtaacgg caccagccag gccagatgac cgtcggtgct gccggtggtg
    47521 agttgcgttg tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc
    47581 agccgacgct taatcgccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc
    47641 tgttcgtcgc cataagcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcattc
    47701 aggcgttcaa gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt
    47761 cgctcgcgca gacattccgg caggtgattc gaaccgatca tccggctttc atcttccagc
    47821 acccacaggc gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt
    47881 tcaaaactcg cctggctaag ttgtggttgt aacgtgcgac caaacgccga accgcgatga
    47941 cgcgccagcc cttccagatc aacaccgttt ggctgttgtt gcactaatag cgttttaccg
    48001 ctgccggtac aaccgccaat cagcactatc ggtttttgtg ccagttcaat ggtcgcctga
    48061 atcgcggtct ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct
    48121 tcatgcagcc agcgttgcac aatatgtgaa cgttgaccgc cacgggcgca gcagagaata
    48181 ccttgcggat tttgcaggca cgctgcccgc caggcgtaca tacgctgctg acgaatttca
    48241 cccgccacca gtttatgccc cagcgccagc gctgcgtctg agccttgctg tttatagcag
    48301 gtgccaacgg cggcgcgttc atcgttattc attaacggca gattgatagc ggcgggcatt
    48361 gcgccttgct caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc
    48421 agggcacgat agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc
    48481 agcttgcgcg ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt
    48541 tgccgaaaat ttctaaaaat ccgctgattt ccggcctgtg ttgggtaaac aaggtcacaa
    48601 tatcttctac cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag
    48661 ggcgcatcgt tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa
    48721 gtgattttgg caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca
    48781 tatcgggaac aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg
    48841 tgcgggcgct gtcttcaata ttgaccgccg gaaagcgacg caactgcgct tctgttagcg
    48901 gctcttcaac gttcgccagc ggatgatccg ccgccatgac aaatcgccat tgcaccgatc
    48961 ctaagggatc aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca
    49021 gcgaaaaacc ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt
    49081 gaaactgggt aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg
    49141 ggttatagag caggttgttg atgacaatat tcacctggcg ttccacgcca tcattcacct
    49201 gttgcagctc gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta
    49261 gatgctcgcc agccgctgtc aacgttacgc tacgagtcgt acggaaaaac agcgctactc
    49321 cggtattctc ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc
    49381 gttctgccgc ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt
    49441 ctggatcgaa catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc
    49501 ctctgattta tcagtatttt tacatgataa tcctgttcaa tttgtggacc aaatctagtt
    49561 ttggaaaaac attccaactt ttgtattgat gttgttctct taaggtttta gattgcctgt
    49621 tattgaaacc aagctgaccg gtcggcggtg gttgaacgga attatgttac aaggacaaaa
    49681 agatgaaact tcaggtatta ccgttaagtc aggaagcctt tagtgcttat ggcgacgtaa
    49741 tcgaaacgca gcaacgggat tttttccata ttaacaatgg cctggtggag cgttaccacg
    49801 atttggcgct ggttgagatt cttgagcaag accgtacgct tatcagcatt aaccgcgcgc
    49861 aaccggcgaa tctgccgctg accattcacg aactcgaacg tcatccgctg ggcactcagg
    49921 cctttatccc aatgaaaggt gaagtgtttg tggtggtcgt ggcgttaggc gacgacaaac
    49981 cagacctgtc aacgctgcgg gcgtttatca ccaacggtga acagggggtg aattaccatc
    50041 gtaacgtctg gcatcaccca cttttcgcct ggcagcgcgt caccgatttt ctgaccatcg
    50101 atcgcggcgg cagtgacaac tgtgatgttg aaagtattcc tgaacaggaa ctctgttttg
    50161 cgtgacgcct gcaaccgact tgcataagat aaactaattg ttcattgttt atgctcactt
    50221 gtaggtcgga gttaacgtag gtatgacgga agttagacgg cgcggcaggc caggacaggc
    50281 ggagcctgtg gcacagaagg gcgcacaggc gttagagcgg ggaattgcga ttctgcaata
    50341 tctggaaaaa agtgggggaa gttcgtcggt tagcgatatt tctctcaatc tggatttgcc
    50401 gctctccacg acctttcgct tgctgaaggt tttacaagca gcggattttg tctatcagga
    50461 cagtcaatta ggctggtggc atataggatt aggtgtcttt aacgtcggtg cggcgtacat
    50521 ccataaccgc gatgtcctct ccgtcgccgg gccgtttatg cgccgcctga tgttactttc
    50581 cggcgaaacg gtcaatgtcg cgatccgtaa cggcaatgaa gcggtattaa ttggtcagtt
    50641 agagtgtaaa tcgatggtca ggatgtgtgc gccactgggc agtcgtctgc cgctgcatgc
    50701 ttccggtgcg ggcaaagcgc tgctttatcc gctggcggaa gaggagttga tgagcatcat
    50761 tctgcaaacc ggtttgcagc agtttacgcc aactacgctt gtggatatgc ccaccttgct
    50821 gaaggacctg gaacaagcgc gtgaactggg ctataccgta gataaagaag agcatgttgt
    50881 aggtctgaat tgcatagctt cagcaattta cgatgatgtc ggtagcgttg ttgccgctat
    50941 ctccatctcc gggccttcat caagactgac agaagatcgt tttgtcagtc agggtgagct
    51001 ggtcagagac accgcccgcg atatcagcac ggcgttggga ctgaaagcac atccatagtg
    51061 tctgtcgcat cccgctctgc ggagcgggtt tttttgacaa aatttaaaag ttggaaaatt
    51121 tttccaatta atagaggtag gaataaaatg gcaaaaatga gagccgttga cgcggcaatg
    51181 tatgtgctgg agaaagaagg tatcaccacc gccttcggcg ttccgggagc tgcaattaat
    51241 ccgttctact cagcgatgcg taagcacggc ggtattcgtc acattctggc gcgtcatgtg
    51301 gaaggtgttt cgcacatggc ggaaggttat acccgcgcaa cggcagggaa tatcggcgta
    51361 tgtctgggga cttccggtcc tgcgggcacg gacatgatca ccgcgctcaa ttccgcttct
    51421 gctgattcca ttcctattct gtgcattacc ggccaggcac cgcgcgcccg tctgcataaa
    51481 gaagattttc aggccgtaga tattgaagca attgctaaac cggtcagcaa aatggcggtt
    51541 acagttcgtg aagcggcgct ggtgcctcgc gtgctgcaac aggcatttca cctgatgcgt
    51601 tctggtcgtc cgggtccggt actggtggat ttaccgttcg acgttcaggt tgcggaaatc
    51661 gagtttgatc ctgacatgta cgaaccgctg ccggtctaca aacctgctgc cagccgtatg
    51721 cagatcgaaa aagctgtaga aatgttaatc caggccgaac gtccggtgat tgttgccggg
    51781 ggcggggtaa tcaatgctga tgcagctgca ctgttacaac agtttgctga actgaccagc
    51841 gttccggtga tcccaacgct gatgggctgg ggctgtatcc cggacgatca tgaactgatg
    51901 gccgggatgg tcggtctgca aaccgcgcat cgttacggta acgcaacgtt gctggcgtcc
    51961 gacatggtgt ttggtatcgg taaccgtttt gctaaccgtc ataccggttc ggtagagaaa
    52021 tacaccgaag ggcgcaaaat cgttcatatt gatattgagc cgacgcaaat tggtcgcgtg
    52081 ctgtgtccgg atctcggcat tgtctctgat gctaaagcgg cgctgacact gctggttgaa
    52141 gtggcgcagg agatgcaaaa agcgggtcgt ctgccgtgtc gtaaagaatg ggtcgccgac
    52201 tgccagcagc gtaaacgcac tttgctgcgc aaaacccact tcgacaacgt gccggtgaaa
    52261 ccgcagcgcg tgtatgaaga gatgaacaaa gcctttggtc gcgatgtttg ttatgtcacc
    52321 actattggtc tgtcacaaat cgctgcggca caaatgctgc atgtctttaa agaccgccac
    52381 tggatcaact gtggtcaggc tggtccgtta ggctggacga ttccggcggc actgggggtt
    52441 tgtgccgctg atccgaaacg caatgtggtg gcgatttctg gcgactttga cttccagttc
    52501 ctgattgaag agttagccgt tggcgcgcag ttcaacattc cgtacatcca tgtgctggtg
    52561 aataacgcct atctcggcct gattcgtcag tcacaacgcg cttttgacat ggactactgc
    52621 gtgcaactcg ctttcgagaa tatcaactcc agtgaagtga atggctacgg tgttgaccac
    52681 gtaaaagtag cggaaggttt aggttgtaaa gctattcggg tcttcaaacc ggaagatatt
    52741 gcgccagcct ttgaacaggc gaaagcctta atggcgcaat atcgggtacc ggtagtagtg
    52801 gaagttattc tcgagcgtgt gaccaatatt tcgatgggca gcgaactgga taacgtcatg
    52861 gaatttgaag atatcgccga taacgcagcg gatgcaccga ctgaaacctg cttcatgcac
    52921 tatgaataag ggagataaat gatgttacgt ttctctgcta atttatcgat gttatttggt
    52981 gaatatgatt ttctcgcccg ttttgagaaa gctgcgcagt gtggttttcg cggcgttgaa
    53041 tttatgtttc cttatgacta cgacattgaa gaattaaaac aggtactggc gagtaataaa
    53101 ctcgaacata cgctgcacaa tttaccggca ggtgactggg cggcgggcga gcgcggtatt
    53161 gcctgtattc ctggccgtga agaagagttt cgggatggcg tagcagcagc gatt
//
LOCUS       DAJTYF010000014        52748 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTYF010000014 DAJTYF010000000
VERSION     DAJTYF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.014"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..354
                     /locus_tag="OO590_000934"
     CDS             <1..354
                     /locus_tag="OO590_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6636401.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO590_000935"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO590_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6636402.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO590_000936"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO590_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6636403.1"
                     /translation="MKHLHQFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3109..3261
                     /gene="mokC"
                     /locus_tag="OO590_000937"
     CDS             3109..3261
                     /gene="mokC"
                     /locus_tag="OO590_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6636404.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO590_000938"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO590_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6636405.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO590_000939"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO590_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6636406.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="OO590_000940"
     CDS             6877..7281
                     /locus_tag="OO590_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6636407.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="OO590_000941"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="OO590_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6636408.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="OO590_000942"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="OO590_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6636409.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO590_000943"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO590_000943"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6636410.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO590_000944"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO590_000944"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6636411.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12152
                     /locus_tag="OO590_000945"
                     /pseudo
     CDS             10704..12152
                     /locus_tag="OO590_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12222..12998
                     /gene="yaaA"
                     /locus_tag="OO590_000946"
     CDS             12222..12998
                     /gene="yaaA"
                     /locus_tag="OO590_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6636412.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13151..13447)
                     /locus_tag="OO590_000947"
     CDS             complement(13151..13447)
                     /locus_tag="OO590_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6636413.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13661..14947)
                     /gene="thrC"
                     /locus_tag="OO590_000948"
     CDS             complement(13661..14947)
                     /gene="thrC"
                     /locus_tag="OO590_000948"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6636414.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14948..15880)
                     /gene="thrB"
                     /locus_tag="OO590_000949"
     CDS             complement(14948..15880)
                     /gene="thrB"
                     /locus_tag="OO590_000949"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6636415.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15882..18344)
                     /gene="thrA"
                     /locus_tag="OO590_000950"
     CDS             complement(15882..18344)
                     /gene="thrA"
                     /locus_tag="OO590_000950"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6636416.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18370..18489)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18425..18490)
                     /gene="thrL"
                     /locus_tag="OO590_000951"
     CDS             complement(18425..18490)
                     /gene="thrL"
                     /locus_tag="OO590_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6636417.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18704..19390)
                     /locus_tag="OO590_000952"
     CDS             complement(18704..19390)
                     /locus_tag="OO590_000952"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6636418.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19790..19930)
                     /locus_tag="OO590_000953"
     CDS             complement(19790..19930)
                     /locus_tag="OO590_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636419.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20026..20742
                     /gene="arcA"
                     /locus_tag="OO590_000954"
     CDS             20026..20742
                     /gene="arcA"
                     /locus_tag="OO590_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6636420.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20801..22153)
                     /gene="creD"
                     /locus_tag="OO590_000955"
     CDS             complement(20801..22153)
                     /gene="creD"
                     /locus_tag="OO590_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6636421.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22211..23635)
                     /gene="creC"
                     /locus_tag="OO590_000956"
     CDS             complement(22211..23635)
                     /gene="creC"
                     /locus_tag="OO590_000956"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6636422.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     VAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(23635..24324)
                     /gene="creB"
                     /locus_tag="OO590_000957"
     CDS             complement(23635..24324)
                     /gene="creB"
                     /locus_tag="OO590_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6636423.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSLGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24337..24810)
                     /gene="creA"
                     /locus_tag="OO590_000958"
     CDS             complement(24337..24810)
                     /gene="creA"
                     /locus_tag="OO590_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6636424.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25021..25890
                     /gene="robA"
                     /locus_tag="OO590_000959"
     CDS             25021..25890
                     /gene="robA"
                     /locus_tag="OO590_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6636425.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25887..26534)
                     /gene="gpmB"
                     /locus_tag="OO590_000960"
     CDS             complement(25887..26534)
                     /gene="gpmB"
                     /locus_tag="OO590_000960"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6636426.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26586..27107
                     /gene="yjjX"
                     /locus_tag="OO590_000961"
     CDS             26586..27107
                     /gene="yjjX"
                     /locus_tag="OO590_000961"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR6636427.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(27192..27518)
                     /gene="trpR"
                     /locus_tag="OO590_000962"
     CDS             complement(27192..27518)
                     /gene="trpR"
                     /locus_tag="OO590_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6636428.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27608..29545)
                     /gene="sltY"
                     /locus_tag="OO590_000963"
     CDS             complement(27608..29545)
                     /gene="sltY"
                     /locus_tag="OO590_000963"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6636429.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     EIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29756..31423
                     /gene="ettA"
                     /locus_tag="OO590_000964"
     CDS             29756..31423
                     /gene="ettA"
                     /locus_tag="OO590_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6636430.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31530..31697)
                     /locus_tag="OO590_000965"
     CDS             complement(31530..31697)
                     /locus_tag="OO590_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636431.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31731..32963)
                     /gene="nadR"
                     /locus_tag="OO590_000966"
     CDS             complement(31731..32963)
                     /gene="nadR"
                     /locus_tag="OO590_000966"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6636432.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32984..34366)
                     /gene="radA"
                     /locus_tag="OO590_000967"
     CDS             complement(32984..34366)
                     /gene="radA"
                     /locus_tag="OO590_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6636433.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34415..35383)
                     /gene="serB"
                     /locus_tag="OO590_000968"
     CDS             complement(34415..35383)
                     /gene="serB"
                     /locus_tag="OO590_000968"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6636434.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35489..36133
                     /locus_tag="OO590_000969"
     CDS             35489..36133
                     /locus_tag="OO590_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6636435.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36161..37177
                     /gene="lplA"
                     /locus_tag="OO590_000970"
                     /pseudo
     CDS             36161..37177
                     /gene="lplA"
                     /locus_tag="OO590_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37209..37472
                     /locus_tag="OO590_000971"
     CDS             37209..37472
                     /locus_tag="OO590_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6636436.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37633..38352)
                     /gene="deoD"
                     /locus_tag="OO590_000972"
     CDS             complement(37633..38352)
                     /gene="deoD"
                     /locus_tag="OO590_000972"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6636437.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38409..39632)
                     /gene="deoB"
                     /locus_tag="OO590_000973"
     CDS             complement(38409..39632)
                     /gene="deoB"
                     /locus_tag="OO590_000973"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6636438.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39684..41006)
                     /gene="deoA"
                     /locus_tag="OO590_000974"
     CDS             complement(39684..41006)
                     /gene="deoA"
                     /locus_tag="OO590_000974"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6636439.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLDGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41133..41912)
                     /gene="deoC"
                     /locus_tag="OO590_000975"
     CDS             complement(41133..41912)
                     /gene="deoC"
                     /locus_tag="OO590_000975"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6636440.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42170..43711
                     /locus_tag="OO590_000976"
     CDS             42170..43711
                     /locus_tag="OO590_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6636441.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDTRCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43683..44546
                     /locus_tag="OO590_000977"
     CDS             43683..44546
                     /locus_tag="OO590_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6636442.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44760..45542)
                     /locus_tag="OO590_000978"
     CDS             complement(44760..45542)
                     /locus_tag="OO590_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6636443.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45539..46612)
                     /locus_tag="OO590_000979"
     CDS             complement(45539..46612)
                     /locus_tag="OO590_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6636444.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46734..46895)
                     /locus_tag="OO590_000980"
     CDS             complement(46734..46895)
                     /locus_tag="OO590_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6636445.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(47022..47627)
                     /gene="osmY"
                     /locus_tag="OO590_000981"
     CDS             complement(47022..47627)
                     /gene="osmY"
                     /locus_tag="OO590_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6636446.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(48020..49609)
                     /gene="prfC"
                     /locus_tag="OO590_000982"
     CDS             complement(48020..49609)
                     /gene="prfC"
                     /locus_tag="OO590_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6636447.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49700..50377)
                     /gene="yjjG"
                     /locus_tag="OO590_000983"
     CDS             complement(49700..50377)
                     /gene="yjjG"
                     /locus_tag="OO590_000983"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6636448.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50392..50838)
                     /gene="rimI"
                     /locus_tag="OO590_000984"
     CDS             complement(50392..50838)
                     /gene="rimI"
                     /locus_tag="OO590_000984"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6636449.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50807..51220)
                     /gene="holD"
                     /locus_tag="OO590_000985"
     CDS             complement(50807..51220)
                     /gene="holD"
                     /locus_tag="OO590_000985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6636450.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51323..52354
                     /gene="rsmC"
                     /locus_tag="OO590_000986"
     CDS             51323..52354
                     /gene="rsmC"
                     /locus_tag="OO590_000986"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6636451.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVITQTGRFKVYRA
                     IMTRQAKKG"
     gene            52631..52717
                     /locus_tag="OO590_000987"
     tRNA            52631..52717
                     /locus_tag="OO590_000987"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52665..52667,aa:Leu,seq:cag)
BASE COUNT        12559 a        14271 c        13233 g        12685 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaaatc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagaaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagagaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cgctaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aaggcagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcgt catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaattgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcta ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggtg
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcacc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ttctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataagg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggagctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcattcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagcggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagcg tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggagcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcgccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc acgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacagt ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc ggtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccgccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgcgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tattgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 cgggaatggg atcgacgcca aacgcggcag cggcagcggc agcggcagcg gcagcggcat
    11581 cctggcctcc gcatccggca gcacaaggga ttgtccagat gattggcatt tttatcgaca
    11641 ccctggtcat ctgtacggca agcgccatgc tgatattact ggcgggtaac ggcacaacct
    11701 acatgccgct ggaaggtatt cagcttatcc agaaggcgat gcgggtgcta atgggttcct
    11761 agggtgctga atttgttacc ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca
    11821 actacattta tgccgaaaac aatctcttct ttttacgcct gaacaaccct aaagcgatct
    11881 ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg caccttgcta agccttccgc
    11941 tgatgtggca actggcagat atcataatgg cctgcatggc tattaccaat ttgaccgcca
    12001 ttttactgct ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac
    12061 tcggcgtgcg cccggtgttt gatccgttgc gttatccgga tatcggtcgc cagctttctc
    12121 cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt
    12181 tttttgctaa agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac
    12241 ctgcgaaaac gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc
    12301 tgttagacaa ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta
    12361 gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact
    12421 ggcagccaga tttcacgccg gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg
    12481 tctacaccgg cttgcaggcc gaaaccttca gcgaagacga tttcgatttt gcccaacagc
    12541 atttgcgaat gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt
    12601 atcgtctgga aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct
    12661 ggggagatat catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg
    12721 tgattaatct ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg
    12781 agattatcaa gccggtgttc ctcgatgaga agaacggcaa atttaagatc atcagcttct
    12841 acgctaagaa agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac
    12901 cagagcaact gactggtttt aatagcgaag gttacttctt tgatgaagat tcctccagca
    12961 atggcgaact ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc
    13021 ggcgtgaacg ccttatccgg cctacaaaag catgcagatt caatatattg cagaattaac
    13081 gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg aatgccggat tgttacccgg
    13141 catttgtcat ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgaggag
    13201 ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc
    13261 attcataatg ttgtttccac cagccgtggt cgcgccagtg acctccatcc cagtaatagc
    13321 cacgattatc acgatcgcct atctgtaatt ttactgacgg gactaacgta atttccgcag
    13381 cctgtgctgc catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct
    13441 ttttcacagg ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg
    13501 atgagaacga cggttgttat tctccgcaat cctaatctct aagttaccgc atttattgat
    13561 aatttctcct ttttccctgt cgtttttctc cataatttct tcataaaaaa gccgggctgc
    13621 ataaaagcaa acccggcctg attgagataa tgaatagatt ttactgatga ttcatcatca
    13681 atttacgcaa cgcagaaaaa tcggcgggca gattatgtga aagcaagggt aaatcagcac
    13741 gttctgccag ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt
    13801 taaatttcgc cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt
    13861 cacgcagcgc acgatacgct acggcagcgt gcggctccga ggtgtagccc agttctttta
    13921 actcacgcat tgtctgttgc gtggtttcat catccacggc tgcataaccc agctctttca
    13981 gttgccagat tttgcggcgg aacaactctt ccacacgcgg ccagttgttc ggctgactca
    14041 cgtccatcgc gttggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca
    14101 ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc accggcagac
    14161 cgaatgactt cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga
    14221 caaccagctg gttgcgcgcc tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa
    14281 tctgcgccag caaacggctg atgttaatcg agttagccga gtttaacccc agcgccactt
    14341 tcagttcttc atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt
    14401 cgatggcaac cgtttcgata ttgccgccca acgtacagaa cagtttttct tgcagtggac
    14461 tgattttgcc tcgtggatag aggataacca ctttcacatt cggcaaaccg tagaaagcat
    14521 gagccactgc agctccggta tcaccggagg tcgcggtcag aatggtcact ggcttatcat
    14581 ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg
    14641 ttggcccgtg gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg
    14701 ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa
    14761 taaacgccga gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa
    14821 tttcagtcag gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca
    14881 acccctgggt tacggcttgc gcaaagctga cctgctcgtt gtgatctttc agattgtaga
    14941 gtttcattta gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa
    15001 aaccttcctg attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat
    15061 ccggcttgtc acatagagcg aacaaggtcg ggccggagcc ggagataccg ctcgctaccg
    15121 cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac cgttcacggt
    15181 agggttcagc gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc
    15241 aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact
    15301 gcgccggtaa aatagcccgg gcttctgccg tcgagacttt aattcccgga tacgccagca
    15361 cccacagcca ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca
    15421 tcaactgcat accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga
    15481 tacgtccttc cagctcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc
    15541 agtgttcatt catcgccatc agcgccgcga ccaccgaaca ggcgctggag cctaagcccg
    15601 aaccgatcgg catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct
    15661 ggcaaaaacg ctcccagcac tgataaacga tattttcccg tggttctgac ggcagcttat
    15721 cggcaaagcg tccgaggttg ttgagactga atgtctctgc cgcctcaacc gtgactacat
    15781 ctccgagcaa tgcaccatca acgggtgcca ccgccgcccc gagcacatca aacccgacgc
    15841 tcatattggc actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat
    15901 gagagggtac gtagcagatc ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca
    15961 tatccgcgca gcaccaacgg cagcggctga taatagtggc tataaaaggc cagggcgttt
    16021 tcgccatttt tcactttgaa cagcggatca ttcccatcca cttcggcaat cttcacgcgg
    16081 cagacaccat cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc
    16141 ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggca
    16201 acatcaccct cggcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc
    16261 tccagttcac gtcccgtttc acgagcgaga atcaatagtt tacgcgccac atccatacca
    16321 gaaagatcat ctcgcgggtc cggttcggta taacccattt cccgcgccag cgtggtcgcc
    16381 tcggagaaac tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga
    16441 atgccggaga acttcaccaa ttcatcacct gcattgagca gattttgcag gttctcaata
    16501 accggtaatc cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc
    16561 gcataacgca actgatgata gtaatccatc gacgaggtgt tggccttttt gttcggcgtg
    16621 acaacgtgga aaccttcgcg caggaagtcg gcatattgat ccgccactgc ctggctggag
    16681 gtgcagtcaa caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc
    16741 ccgagattaa acggctcttt ggcttgtgca agttcttcct gccagttttc cagattaagg
    16801 ccatgcacac tagtgagcag agccttcgag ttggcaacgc cgcagacacg taagtcgata
    16861 tgtttattct tcagccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg
    16921 ccaccgacgc caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga
    16981 gtaacgcgca cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca
    17041 gaagatccct gagcaatggc gacaatgttg atattggcgc gggccagcgc ggcaaagaat
    17101 ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc
    17161 cgttccgtca ccgccagcgg ctccagtaag ccttctttca gttccaggta gaactcttcc
    17221 tgcattgccc gttcagctcg cacacagtcg ctttgtggaa cgcagaaact gatactgtat
    17281 tcggaagatg attgcgtaat cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag
    17341 acgcgcgccg ccatgccaac catccctttc atccccgggc cggaaacgct gaacattgcc
    17401 atgttattca gattggaaat gcccttgacc ggtaattcgt cttcatcacg gctggcacca
    17461 atgagcgtac ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac
    17521 tgggcgatgg gggtaatggt gcgggggtga agaactttag cgccgaagta ggaaagctcc
    17581 atcgcttcct ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg
    17641 caggtataga ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca
    17701 gccagcaccg ccgcggagta gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt
    17761 tcattaccgg cggtgaaacc tgccatcaac accatgtgat cagccggaat gcggcttgcc
    17821 gcaatacggc gggtggactc agcaatatcg acggtagatt cgaggtaatg ccccactgcc
    17881 agcagttttt cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg
    17941 gccataatgg cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc
    18001 gggcactgcc ccaacaaact aatgccatgc aggacatgtt ttatttgggc aaattcctga
    18061 tcgacgaaag ttttcaattg cgccagcggg aaccccggct gggcggcggc gagtcccgtc
    18121 aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta
    18181 atggtttttt caatcatcgc caccaggtgg ttggtgattt tggcgggggc agagaggacg
    18241 gtggccacct gcccctgcct ggcattgctt tccagaatat cggcaacacg cagaaaacgt
    18301 tctgcatttg ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta
    18361 cctttggtcg aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta
    18421 cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa
    18481 tgcgtttcat ggatgttgtg tactctgtaa tttttatctg tctgtgcgct atgcctatat
    18541 tggttaaagt atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc
    18601 agttcagaag ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt
    18661 gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt tttttatttg gtgatatttt
    18721 tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca
    18781 ggcgttgctg taaccagtcg accagtttta tgtcatctgc cactgccaga gtcgtcagca
    18841 atgccatgac tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt
    18901 tcgccggttg ttgtattaat gttgctaatt gatagcaata gaccatcacc gcctgcccca
    18961 gattgagcga aggataatcc gccaccatcg gcacaccagt aagcacgtca gccaacgcta
    19021 actcttcgtt agtcagcccg gaatcttcgc gaccaaacac cagcgcggca tggctcatcc
    19081 atgaagattt ttcctctaac agcggcacca gttctactgg cgtggcgtag taatgatatt
    19141 tcgcccgact acgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac gattcagcca
    19201 gtgtcgggaa aactttaata ttatcaataa tatcactaga tccatgtgcg acccagcggg
    19261 tggctggctc caggtgtgcc tgactatcga caatccgcag ttcgctaaaa cccatcgttt
    19321 tcattgcccg cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc aggataatcg
    19381 ttatacgcat attgccactc ttcttgacca aataaccgcg aacagggtga tcactgtcaa
    19441 cttattacgc ggtgcgaatt tacaaattct taacgtaagc cgcagaaaaa gccctttact
    19501 tagcttaaaa aaggctaaac tattttctga ctgcactaac ggtttaattg ttaaaaaatg
    19561 ctacatatcc ttctgtttac ttaggataat tttataaaaa ataaatctcg acaattggat
    19621 tcaccacgtt tattagttgt atgatgcaac tagttggatt attaaaataa tgtgacgaaa
    19681 gctagcattt agatacgatg atttcatcaa actgttaacg tgctacaatt gaacttgata
    19741 tatgtcaacg aagcgtagtt ttattgggtg tccggcccct cttagcctgt tatgttgctg
    19801 ttaaaatggt taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca
    19861 cgaccaagct aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta
    19921 ctttagtcat gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact
    19981 tttgtacttc ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac
    20041 attcttatcg ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg
    20101 gaaggctatg atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa
    20161 tatgacatca acctggtgat catggatatc aatctgccgg gtaagaacgg tcttctgtta
    20221 gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac
    20281 gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc
    20341 aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt
    20401 actgtcagcg aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac
    20461 atcaacagcc gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag
    20521 ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg
    20581 ctgaagaaaa tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc
    20641 cgccgtattc gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt
    20701 cacggtgaag gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca
    20761 aaaaaacggc gctttttagc gccgttttta tttttcaacc ttatttccag atacgtaatt
    20821 catcgtccgt tgtaacttct ttactggctt tcattttcgg cagtgaaaac gcataccagt
    20881 cgatattacg agtcacaaac atcatgccgg ccagcgccac caccagcaca ctggttccca
    20941 acaacagcgc gctatcggca gagttgagca gtccccacat cactccatcc agcaacaaca
    21001 gcgcgagggt aaacaacatg ctattgcgcc agcctttcaa taccgcctgc aaataaatac
    21061 cgttcattaa cgccccaatc agactggcga ttatccatgc cacggtaaaa ccgatatgtt
    21121 cagaaagcgc cagcaagagc aaataaaaca tcaccaatga aagccccacc agcaaatatt
    21181 gcattgggtg taaacgttgc gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca
    21241 gtgcaatcag cagaatggcg tacttagtcg cccggtccgt taattggtat tgatcggctg
    21301 gcgtcgtcac tgcgacgcta aacgcgggga agttttccca gccggtatca ttgcctgaag
    21361 caaaacgctc accgagatta ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg
    21421 actcgctagc ttcccgtttg gctggtagaa aatcacctaa aaaactggga tgcggccagt
    21481 tgctggttaa ggtcatttcg ctattacgcc cggcaggcac cacagaaaga tcgccggtac
    21541 cgcttaaatt cagtgccata ttcagctgca ggttctgctt ccgccagtcc ccttcgggta
    21601 aagggatatg cacgccctgc ccgccttgct ctaacccggt accgggttca atggtcagtg
    21661 ccgttccgtt aacttcaggc gctttcacca caccaatacc acgcgcatcc ccgacgctaa
    21721 tcacaataaa gggcttgcct aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa
    21781 catcgaaatc ggcttttagc gttaaatcac tgtgccagac ctgaccggta taaatcccta
    21841 tcttgcgttc ttccacgttc tgattgccat caaccatcaa tgactcaggt aaccaaaaat
    21901 ggataaaact tcgtttccgc tccacggttt tatcctcttc ctgcaccgta taaagctcgg
    21961 tcacaggaat agcgataagc ggcccaacga gtttttgcgg cccgctggta ctttggcgaa
    22021 tcgcatcttc cacatcgcta cggtaatcgg cacgttcgac aatcacttgc cgaatcagca
    22081 ttatcggaat caacaacagc aatactgcac caaacaaggt ggtcattttc cagaacaggg
    22141 gggatttcaa catagtcttc tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa
    22201 gaatttgaag ctatgtgaag ttacggtgaa gtcgtagcga ggccagcacg ccaccttcct
    22261 gcacgttgcg cagcgtgact tcgccgttaa acaaacgggc gacctcactg acgaacgcca
    22321 accccagacc gctgcttttt tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa
    22381 tacgtgacag cgcgtagtca ggaataccac taccggtatc cagcacctta agcgtgacgt
    22441 gttcctgatc cacttcggcg cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg
    22501 tgttatccag taaattcccc agcgcctgat ccagtaacgc cggttcagca gcaacattaa
    22561 cctcggtggg cataacatgc aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt
    22621 cgctgacgcg gcgaaataat gccgccacat caacagcagt cagaacgact tcctgacgat
    22681 tctccagtct tgcctggcgt agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg
    22741 tcagaatgtt gtcagtaaaa cgagccacta cttcaggcgg cggaccttcg cgtaaaattt
    22801 ccgccacgcc acgaatcgcc gccagtgggc tttttagctc atgggttaac gcataaacat
    22861 actgctcaat atagtttttc ccttccagct tcacgcgcat actttccagc gcctgcgcga
    22921 gtttacgcag ctcgctacta ccgagatcgg gaagaggaac gggcttattg tcagtgacgg
    22981 aatcagcata gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc
    23041 caatcaccag tgcaatcccc aacaaaatgg cgctggccca taaaattcgc cgctcgctgc
    23101 gtttaatgac cggagccatc gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc
    23161 gcgagccgcc cataatcggc gcggcaacat acatcaccga actttcggga tcggcaggat
    23221 tttgcaacgt gctgcgcgca ccatactgac cacgcaacgt tagccagacg tcattccagc
    23281 gcgaataatc ctgtccaacg gctttatttg ccgaatcgaa caatactttg ccatgcgcat
    23341 cggtcatata gacatgatat tcattgcgca ctttgttaat gccaccaata ttggcgcgaa
    23401 acgggcgatg ttgtagctga ttaaacgcct gcgccagttg cccatgcgtt gggtccccag
    23461 agagcaaatc gggacgcgcc agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct
    23521 ccgttgctct tcgcacgccc ggtttaactt ctttgacaaa aatagccagt acgaaccagg
    23581 ccgccaccgc caccagtaaa aaatagccca gcagcaaccg catgccgata cgcattacaa
    23641 gcccctcagg ctatatccca tgccgcgatg agtattaatc ggtgaaagat cggggttgat
    23701 ggcgcgcagc ttggcacgca gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc
    23761 ctgcgcatct tcccacacgc tatccattag ttgctggcgg gaccagacgc ggcctagcga
    23821 cttgagtaac gtcttcagca ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa
    23881 ccagctgatc tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga tgacgggaga
    23941 cggcgtcgag aacttcttca cccgacgcag taaggtgcgc accctggcgc acacttcgcg
    24001 gggtgaaaac ggtttagcca catagtcgtc agcgccaatt tccagcccaa gcaggcgatc
    24061 gacctcttca cttcgggccg tcaggaacag tacaggtaac gccggatgga gcgccagtaa
    24121 ctggcggcac aattcaaagc cactgatatc aggcaaacca acgtcgagaa tcatgacgtc
    24181 gggaacctgc tggcgagctt tatccagcac cggcaaacct cgctcaaaga cctcgacggc
    24241 aaaaccttcc tgctgcaaca tgtagaccag cgtgtcggct atcccttgct catcttccac
    24301 taaccagacc gtttcccgtt gcataaatcg cctctgttat tgccgccacg gcatgacagg
    24361 aaccgcgcta atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt aagccagata
    24421 agcgagcgcg ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact taaagaccag
    24481 ggacgtgcgt tttttgaata ctacctcgcc ctgagctttg ccgtttttaa tacgatccga
    24541 cagttcaatc ggcccgactt gctgacaaga aatggccgca tcggaggtat cttctgccag
    24601 gcccaatccc cctttaatac caccggtttt cgcccggctc acataacagg tgacattttt
    24661 cacatcggga tcgtcaaagg cttccacaac aattttgtga tccgggccga tcattttaaa
    24721 tacggtgtcg accgaaccaa tctcttctgc atgagccaat ggccccagca ttatcaggct
    24781 taaagacagg atcaaatgct tgtatttcat attgttacca tttttatcta ttacacgagc
    24841 aattagttcg tcacggtaaa taacatattg attaagaaca aaatctcaat tcttttattt
    24901 ccgtcaggcc ctaaaacata ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa
    24961 aatgctatta tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt
    25021 atggatcagg ccggcattat tcgcgacctt ttaatctggc tggaaggtca tctggatcag
    25081 cccctgtcgc tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca cttacagaga
    25141 atgtttaaag atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg
    25201 aaatcggcgg tcgcactacg cctgactgcg cgtccgattc tggacatcgc gctgcaatac
    25261 cgcttcgact ctcaacagac atttacccgc gcattcaaga agcagtttgc ccagactcct
    25321 gcactttacc gccgttctcc tgaatggagc gcctttggta ttcgcccgcc gctacgtctg
    25381 ggtaaattca caatgccaga gcacaaattt gtcaccctgg aagatacgcc gctgattggt
    25441 gttacccaga gctactcctg ttcgctggag caaatctctg atttccgcca tgaaatgcgt
    25501 tatcagttct ggcacgattt tctcggcaac gcgccgacca ttccgccggt gctctacggc
    25561 ctgaatgaaa cgcgtccgag tcaggataaa gacgacgaac aagaggtatt ctataccacc
    25621 gcgttagccc aggatcaggc agatggctat gtactgacgg ggcatccggt gatgctgcag
    25681 ggcggcgaat atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc
    25741 ctgacggtat acggaacgtg catgccaatg ctcaatctga cgcgccgtaa aggtcaggat
    25801 attgagcgat actacccggc agaagacgcc aaagcgggag atcgcccaat taatctgcgc
    25861 tgtgaactgc tgattcctat ccgccgttaa cgctgcagct catctaatgc aggggcgtcc
    25921 agatgcgaga tgtcccctgc ggtttcaacg acccagccag acgccagcca caggctttcc
    25981 tgataatcca cgcgcgaaat agaacagtta cgcagacgta agcggcgctc tgcccatgct
    26041 ggtaatccga gaatcgtact caccagacat cccagtgcaa taccatggct caccagcagc
    26101 ggtcggcttc cctgcggtaa gtcccggcaa gattccagtg ccgcattgac gcgatcgctg
    26161 agctcctgca ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc
    26221 cgacgccagt tctcttcttc ttcggtcaga gaatcgatat gtcttttttc cagcacaccc
    26281 atgtttaatt cacgcaggcg agaatcaaag atgatgtcac agccgcaggc ctgggcgatg
    26341 atttccgccg tacgccgggt gcgtcctaaa tcgctactga taatatgcgt aatgccaagc
    26401 tctttggcac gggttgccac ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg
    26461 tcagactggc cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg gcggactagg
    26521 tatacctgta acatgctttt tttccgtata ctgctgtaac gttaattttc tgagtaatgc
    26581 tgattatgca ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag gccattctgc
    26641 aggcatttca cgagatcttc ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga
    26701 gcggtgtacc ggaacaaccc tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg
    26761 tagcaaatgc ccgccgttta cttccagagg ctgatttttg ggtggcgatt gaagctggca
    26821 ttgatggcga cagcactttc agttgggtgg tgattgaaaa cgccagccag cgcggcgagg
    26881 cgcgttcggc aactttgccg ttacctgcgg tgattctgga aaaagttcgt gaaggcgagg
    26941 cgcttggtcc cgtgatgtcg cgttataccg gtattgatga gattggccgt aaggaagggg
    27001 cgatcggcgt gtttaccgcc gggaaactca ctcgcgccag cgtctatcac caggcggtga
    27061 tcctggcgct gagtccgttt cacaatgccg tgtatcaggc attgtaggac ggataaggcg
    27121 ttcacgccgc atccgacatt cggtgcacga tgcctgatgc gacgctgccg cgtcttatca
    27181 ggcctacaaa atcaatcgct tttcagcaac acctcttcca gccactgacg cagctccacg
    27241 ggcgcggctt tcaggctgtt agatccacgc gtaatcgtcg cgatgcccgc gccgagttca
    27301 ttttttaact cacgctgact catttcgcca cgcaacagct cttcgacaat acgcacgcga
    27361 gtccccaacg cttcgcgctc atctggcgtc agcatcaggt ttaacaacgg taaatggaga
    27421 tcgttttggt aggcattctt aagcaggtcg acaaaacgta accactcctg gtgacgctgt
    27481 tctgccatcg ctgctgaata gggtgattgt tgggccataa tatgtcgcca ttgttagcgg
    27541 gggaagcaaa atgactcccc cggttgtact cgctaaagag tacgatagca tatcataaac
    27601 gtgcggatca gtaacgacgt ccccattccg tggcgctcat caacgtcggt ttatccccca
    27661 tgaaatagcg gtagtaagca tcataagcca gcacgttctt cacataaccg cgcgtctcgg
    27721 agaacggaat actctcgaca aatgccacag cgtcgatacg cccggcgctg ttgccaagcc
    27781 aggttcgcac ccgtccaggt ccggcgttat aagctgctga ggagaaaata cggttattgc
    27841 caaactgctg ataaacatat tgcaggtaac tggtgccaat gttgatgttc gtttccggat
    27901 ccagcaattg cccagggctg ctataaccag gaatagagaa catcttcacc gtatgggtcg
    27961 ctgtaccagg cataatctgc atcaggccgc tggcccctac cggtgatttc actttcggat
    28021 tccaggcgct ctcctgacgg gcaatcgcca tcgcatagct ttgcgggatc tctttcccac
    28081 tggtgtaacg tttgaaaaga tcgttgtaag ccagcgggaa tcgctcttcc agatgatccc
    28141 aaagcttccc ggcgatcgtt gcctgaacgc taagatccca ccattggtta ttgaaagcat
    28201 accgagccag ttgagcctgc tctgtttttg acttgctctt caccagattg gcccactcgc
    28261 tacgcgcggt attatcgaga ttccagtaca tcaactcgcg cacgcgcgcc atctccggcc
    28321 cctgagtcag ggtgctgtca acattctgcg gcgctttatc aatcttcagc tcatactctt
    28381 caccgatgcg ttgtgctgca accatcgggt agaaaccacg ctgttgcatg agttgatgca
    28441 aaatctcttt cgcttcagct tcacgtccgc gttccagcaa taaatccgcc tgccagtaac
    28501 gccattcatc tttctctttt gcttccatcg gcagacgcgc cagccaggta ttcaggccgc
    28561 gacgatcgcc ggtgccaagc gccattcgca cacggcgttc aatcaacgac gtggattgcg
    28621 agcgcataat ggcgtcatcg cgccatttcg cctgctcatc ggtgacatca ttgcccatca
    28681 aacgccaggc gacgatatcg cgcagctcct gaatctcatc ttcattaagc tgctgcgcct
    28741 gggcaagcga cgggatcatc agccgtgcat tttcggcatc ctgccgcgcc acactggcaa
    28801 acgccaccgc cgccatttga cgggtaaaat cggtcgcgcc ggttgtacgc gcgaaggtca
    28861 gtaccgtatt agggttgttc gccagtgaaa tgattgccga ggcgatagtc tggtaatcgg
    28921 caggcatctg ccctgccagc actgttacca ggcctgtgtt acccgctttc atcgccagac
    28981 ggatacgctc taaatacgcc agcggatctt gtttacctga cgcacgccag acgctaaata
    29041 acttgtcaca ggcgttaggc tggctcttgc cggttagcca cagctctttc gccccttgcc
    29101 aggcttcttc actctgcccg gtgttccatt tcgcatagta gtaattacat tgcgcttcgg
    29161 tagttccggg cttttccggg ctaaaggcta acaagccacg ccagtcttca cgccgcgcca
    29221 gttcattgac gaaacgagat tgcagcgtgc gagcgggagg aagcgtgggg ttagcgcgaa
    29281 caaagttagt gaccgtcacc gccggttgat tcatcaggtc atcggtgatc tggcggtatt
    29341 ccaggtaggg ataaagcgga taatccttca gtccaggcat catttgttcg accacatcca
    29401 tctgtcgatt atcccaggcc tgcttgattt gcgcgtaacg gctacgctgc tcatccagtg
    29461 agtcggctcg cgccacgctg ctgaccgtca gcagacagac accggcagcc aacagccgcc
    29521 aggtaacttg tttggctttt tccacaagcg catcctctaa gtgtaaatac atcaatgcag
    29581 cgtcataccg cgtaatagat ttatgctaac cagtcattgc cgtttacgcc acgttacgga
    29641 cactttttta cttttactgc gaaggtgatc gaccaactgg gattaggcgg ggaaacaggc
    29701 tacacttcgc ctttgaaaac tcatcatcac gataaacata gaggcgaagt ccaacgtggc
    29761 tcaattcgtt tataccatgc atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt
    29821 gaaaaacatc tctctgagtt tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg
    29881 cgcgggtaag tccaccctgc tgcgcattat ggcgggcatt gataaagaca tcgaaggtga
    29941 agcgcgtccg cagccagaca tcaagattgg ttatctgccg caggaaccgc agctgaaccc
    30001 ggaacacacc gtgcgtgagt ccattgaaga agcggtttca gaagtggtta acgccctgaa
    30061 acgcctggat gaagtgtatg cgctgtacgc cgatccggat gccgattttg acaagctggc
    30121 cgctgaacaa ggccgtctgg aagagatcat tcaggctcac gacggtcata acctgaacgt
    30181 acagctggag cgtgcggcgg atgcgctgcg tctgccagat tgggacgcga aaatcgctaa
    30241 cctctccggt ggtgagcgtc gtcgcgtggc gttgtgccgc ctgctgctgg aaaaaccaga
    30301 catgctgctg ctcgacgaac cgaccaacca cctggatgcc gaatccgtgg cgtggctgga
    30361 acgcttcctg cacgacttcg aaggcaccgt ggtggcgatt actcacgacc gttacttcct
    30421 cgataacgtt gcaggctgga tcctcgaact ggaccgcggt gaaggtattc cgtgggaagg
    30481 taactactcc tcctggctgg agcagaaaga tcagcgtctg gcgcaggaag cttcacaaga
    30541 agcggcgcgt cgtaagtcga tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg
    30601 ccgccagtcg aaaggtaaag cacgtctggc acgctttgaa gagctgaaca gcaccgaata
    30661 ccagaaacgt aacgaaacca acgaactgtt tattccacct ggaccgcgtc tgggcgataa
    30721 agtgctggaa gtcagcaacc tgcgtaaatc ctacggcgat cgcctgctga ttgatgacct
    30781 gagcttctcg atcccgaaag gggcgatcgt cgggatcatc ggtccgaacg gcgcgggtaa
    30841 atcgaccctg ttccgtatga tctccggtca ggaacagccg gacagcggca ccatcacttt
    30901 gggtgaaacg gtgaaactgg catcggttga tcagttccgt gactcaatgg ataacagcaa
    30961 aaccgtttgg gaagaagttt ccggcgggct ggatattatg aagatcggca acaccgagat
    31021 gccaagccgc gcctacgttg gccgctttaa ctttaaaggg gttgatcagg gtaaacgtgt
    31081 tggcgaactt tccggtggtg agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg
    31141 cggcaacatg ctgctgctcg acgaaccaac caacgacctg gatatcgaaa ccctgcgcgc
    31201 gctggaaaac gccctgctgg agttcccggg ctgtgcgatg gttatctcgc acgaccgttg
    31261 gttcctcgac cgtatcgcca cgcacatcct ggactaccag gatgaaggta aagttgagtt
    31321 cttcgaaggt aactttactg agtacgaaga gtacaaaaaa cgcacgctgg gcgcagacgc
    31381 gctggagccg aagcgtatca agtacaagcg tattgcgaag taatgcgtaa aatgccggat
    31441 gcggcgcgaa cgccttatcc ggcctacaaa accagcaatt tcaatacgtt gtaaaactgt
    31501 aggcctgata agacgcggta agcgtcgcat caggcattga gcaccgaatg ccggatgcgg
    31561 cgtgaacacc ttttccggtc tacaacagcc ttagcatcct caatttcgtg gcgggtcacc
    31621 cgtgcaggtt ttttcaaacc ttgtttaatc tcgacagcct cttccagaga ggttttcaac
    31681 tcatcaaaaa aactcatttt atacctcgcc tttgagcagt ttcgtcacgg ttatccttgc
    31741 tcccccatca tctcccgcac cagctccacg cagcgcagga aacggctgtc gtaatcgtcc
    31801 tcttcaaccc gcacgaattc gatattgttc tcttccagca tctccaccag caagttctgg
    31861 aactctttgc gatccaccga actgccgagg ctgcgtaaac catccgccac ccacggcgtg
    31921 ttgttctcca gcaggatcac cagatcgaaa cggtattcat caatcagcgc ctgcacgaac
    31981 ggatgctcac gcccttcgta ctttttgcag aacgcctggg tagtgacaaa atcggtgtca
    32041 ataaacgcca ctttattggc gtatttcact gcaaaatcaa tgtattgtgc gtggcccaac
    32101 gcgattttat cgtagtcgga atactgcaat gcgatctcat caccgccgag gtgtgaaaag
    32161 acataatcgc ggccatattc ccacgcactg gtggtgttga agatattggc aagtttgttt
    32221 accagggtgg atttaccgct cgactcgccg ccaaggatcg ccacggtacg cacaaaaaac
    32281 ggcttcactt cggtaggaat atattcccag tagcggaacg ggttttcgcg gatctgcgca
    32341 ccgctgatac tcataaaggt acgtttcgga tcgaccagca ccgtctcgat ccccagatgt
    32401 tccatatact gtggcgcatc ggcttcttcc gaggtgtaga tcaggtccgg ctggatcccc
    32461 ttttcagcca taaacttttt gatgccgttg ctccacacat cccagccatg cggatacggc
    32521 tccatgccct cttcgttgaa agcatgaata cggatatttt tctgatattt aaaggtttgc
    32581 aatagccagc gcagacgatc cggcaccgtg ggctgctgcg acatggcgct gtcttcaaac
    32641 aacgcgcggt cgcgggtatc gtcaaaaccc ataatgatat gcagctcgtc aacctggcta
    32701 caggcgcgct ggataaggta gatatgtccg gtatgcagcg ggtagaactt accgaatacg
    32761 acaccaatgg ttttcttctg ccggggaaat tcaagcccca aaaaacggtg caatgcctcc
    32821 agcttttgcg cgctggggct tttaattttg gcattcagta actggcttaa ataccctttg
    32881 gtcataccgc tggcatcagc tacctgctgt agcgtgcagc cctgttgctt gatggcagtt
    32941 ttcaggtaat caaatgacga catatctccc tccgtatctc tcattataag tcgtcgaaca
    33001 cgctaagcgc gtcggagagt tttttaacgc caaaaatctg catcccttcc ggcgcttttt
    33061 tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc gtgtttcgcc gcttcagaaa
    33121 tccgctcctg gccactgggc accgggcgga tctcccctgc cagcccgact tcaccaaaca
    33181 ccaccagatc ctgtggcagc ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg
    33241 ctaagtcggc gctggtttcg gttaccttca cgccgccgac cacgttcaca aacacatcct
    33301 gatcggccat ttgcagacca ccgtgacggt gcaagacagc cagcaggatt gccagacggt
    33361 tttgttccag ccccactgcc acgcggcgcg gattcgccat tatcgagtga tcgaccagcg
    33421 cctgaatctc caccagcaac ggacgcgttc cttcccacac caccatcacg gagctgccgg
    33481 aggtcacttc atctccgcga cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc
    33541 cctgttcggt catcgcgaag acgcccagct cattcaccgc gccgaagcgg tttttatggc
    33601 tgcgtaaggt gcgaaaacga gagtcggcat cgccatccag aagcaccgaa cagtcgatac
    33661 agtgttccag cactttcggg ccagccagcg aaccatcttt ggttacgtgt ccaaccatga
    33721 caatcgccac accgcgcgtt ttggcgaaac gcgtcagata agccgccgtt tcacgcacct
    33781 gcgccacgct accaggcgat gattgtacat ccgccatatg catcacctgg atcgagtcaa
    33841 ttaccatcag cttcggttgc tcttcttcgg caatcaggca gatctgctcg atgctggttt
    33901 ccgacaacat attgagattg tcagtcggca ggccaaggcg atgagcgcgc attgccacct
    33961 gttgcagcga ctcttcgccg gtgacataca gcgttttcat ctgctgggcc agtttgcaca
    34021 gcgtttgcag caacagcgtg gatttccccg caccagggtt accgccaatc agaatggcac
    34081 ttcctggcac cacgccgccg cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac
    34141 gcggcagctc ttcaaggctg atatcggaga gtttctggac ttttgccacc ccggcgctac
    34201 cggcatagcc gctgagacgc tcgttacgcg ccaccgttgg cgacgcagca agacgcacct
    34261 cggtgatggt gttccaggca tgacaggcac tgcactgccc ctgccagcgc ggataatcgg
    34321 ccccgcattc attacaaaca aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta
    34381 atgctgtgcc gcccgcagga tgacgggcag gcaattactt ctgattcagg ctgccagaga
    34441 ggatgcagaa tacccccatc aggtcagcgt gacggatggt gacttccgcc ttttcattca
    34501 cttttggctt ggcatggtag gcaatcccca gccctgctgc tttgatcatc ggcaggtcat
    34561 tagctccatc gccaatcgcc acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc
    34621 gagtcagagt tttcgctttg tactgtgcgt ctacgatgtc gccgatcaca ttgccggtaa
    34681 atttaccgtc catgatctcc agttcattgg ctaccacggc tgtcaggcgc agcttgtcgc
    34741 gcaggtattc agcaaagaaa gtaaagccgc cggaggcaat cgccactttc cagcccagcg
    34801 tttccagctt gagcaccagc tgcgttaagc ctggcatcag cggcagattt tcacgcacct
    34861 gttgcagaat attggcgtca gcgcctttca gcgtcgccac gcggctgcgc aggctggcgg
    34921 taaaatcgag ttcgccgcgc atcgcccgtt cggttacctc cgctaccatc tcgcccgttc
    34981 cggccagctt ggcaatttca tcaatacatt caatctggat ggcggtggaa tccatgtcca
    35041 tcaccagcaa acccggcgtg cgcaggtgcg ggattttccc cagcggggcg acatccagct
    35101 gcgcttcgtg ggccaggcgt gtagcccgtg cggtgagtga acctgccaga cgaatcacct
    35161 gataatcttc cacgcaccag gcggcaacaa tcaccatcgc cgcacccagt ttgctctggt
    35221 attgggtcag acgctgttta tccagcccac gaccatacag cagccagccg ctacgagctg
    35281 cgtggtaatc cagtggcatc acttcatcac cacttaatga aagaggcaga cccggccata
    35341 aagagacatc ttcaggcagg tcgcaccagg taatgttagg cattaaggct cctgtaaaat
    35401 cgttcgaagc agggaaaata acgcatgagg ctaccttgta tccattgctt ctggcaacat
    35461 taagtctcaa attttcaaag ggtggaagat ggctcgcaca aaactgaaat tccggctaca
    35521 tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg gcactgatgc agggagcgtc
    35581 atggtttagt caaaaccacc agcgacagcg taatccacag ctggaagaac tggcccgcac
    35641 cctggcgcgt caggtgacgc tgaacgttgc accgctgatg cgtaccgact caccggatga
    35701 aaaacgcatt caggcgatcc tcgatcagtt aacggatgaa agccgtatcc tcgacgcggg
    35761 tgtgtatgac gaacaaggcg atcttatcgc acgttctggc gaaagcgtcg aagtgcgcga
    35821 tcggctggcg ctcgacggta aaaaagcagg cggctatttt aaccagcaga ttgtcgagcc
    35881 aattgcgggt aaaaacggac cgctcggcta tctgcgcctg acactcgaca cccatacgct
    35941 cgccaccgaa gcccaacagg tggataacac cactaacatt ttacgcctga tgttgctgct
    36001 ctcactggca atcggcgtag tgctgacccg cacgctgcta cagggtaaac gcacccgctg
    36061 gcagcaatcg cccttcctgt taaccgccag caaaccggtg ccggaagagg aagaaagcga
    36121 gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct
    36181 catctctgac tcttacgacc cgtggtttaa cctggcggtg gaagagtgta tttttcgcca
    36241 aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat gccgacacgg tagtaattgg
    36301 tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg atggaagaag ataacgtccg
    36361 cctggcgcgg cgcagtagcg gtggcggcgc ggtgttccac gatctcggca atacctgctt
    36421 tacctttatg gctggcaagc cggagtacga taaaactatc tccacgtcga ttgtgctcaa
    36481 tgcgctgaac gcgctcggcg tcagcgccga agcgtccgga cgtaacgatc tggtggtgaa
    36541 aaccgtcgaa ggcgaccgca aagtctcagg ctcggcctat cgcgaaacca aagatcgcgg
    36601 cttccaccac ggcaccttgc tactcaatgc cgacctcagc cgcctggcaa actatctcaa
    36661 tccggataaa aagaaactgg cggcgaaagg cattacttca gtgcgttccc gcgtgaccaa
    36721 cctcaccgag ctgctgccgg ggctcaccca tgagcaggtt tgcgaggcca taaccgaggc
    36781 ctttttcgcc cattatggcg agcacgtgga agcggaaatc atctccccgg acaaaacgcc
    36841 agacttgcca aacttcgccg aaacctttgc ccgtcagagt agctgagagt ggaacttcgg
    36901 tcaggctccg gcattctcgc atctgctgga tgaacgcttt agctggggcg gcgtggaact
    36961 gcatttcgac gttgaaaaag gccatatcac ccgcgcccag gtatttaccg acagcctcaa
    37021 ccccgcgccg ctggaagccc tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat
    37081 gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg gaccaggaaa aagagctacg
    37141 ggagttgtcg acgtggatag cgggggcggt aaggtaactc gctgattaaa atatccaagg
    37201 aaggaaatat gatcgatctg gaaaaccagg aacgggagat tatcaattta atgttcagcc
    37261 agcgtatttc ctggctagcc gccgtgcgta tcaggcataa attatcgctt gctgaagtaa
    37321 gtaaaatgtt aggtatctcg attaattctc taaaacagat cgaaaagacg gaacggctta
    37381 gctcaaacat caaaagcaaa atggcagaaa tttatggttg tccaccagag ttgctgatat
    37441 gcccttcctg gatgacggct gaacataaat gaatagattc gtgaatagat acttgaatgt
    37501 attttatttt ctatccatat gaaaaataat agtaaatcta taaatcagtt gctattgatg
    37561 aatagatact aaaaagccgg agcagtcccc cactccggct taacacgaca atcgcctttc
    37621 agcgaaacac aattactctt tatcgcccag cagaacggat tccagtgcga ttttgatcat
    37681 gtcgttgaag gtggtctgac gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc
    37741 agacacggtg cagatggtca gggctttcgc gccaaactct gcagccacgc cgtagatacc
    37801 agccgcttcc atttccacgc cgaggatgcc gtatttttcc atcacgtcga acatttcgcc
    37861 gtccggagag tagaacaggt cagcggagaa caggttaccc acgcgagcat caacgcccag
    37921 tgctttagct gcatctactg cgttacgcac catgtcaaag tcagcgatag cggcaaagtc
    37981 atggtcttta aaacggatgc ggttaacttt ggaatcggtg caggccccca taccgataac
    38041 gacgtcgcgc agttttacgt gcggcagaac tgcgccacag gaaccaacgc ggataatttt
    38101 cttcacgccg aaatcggtga tcagttcttt ggtgtagatg gagcaggacg ggatacccat
    38161 accgtgaccc attacggaaa ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc
    38221 gcgaacgttg ttcacttcac gggcatcttc aaggaaagtt tcagcaatat acttcgcacg
    38281 cagcgggtcg cctggcatca aaactacgtc agcgaaatcg cccatttctg cattaatgtg
    38341 tggggtagcc attgttttat cctttttgtg acataacaaa ggcggagtca acgctccgcc
    38401 caaatccatc agaacatggc tttgccatat tccatatcag aagtaccaaa atattttgcc
    38461 agagtctggc cgatatccgc gaaggtttca cggtgaccca gtgagcccgg ttttactttc
    38521 gggccatata ccagtaccgg aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga
    38581 tcgcaaccgt ggtcagcggt gaggatcagg atgtcgtcat cgcgcagcag agacatcagc
    38641 tccggcagac ggcggtcgaa cagttccaga cccgcagcat aaccggcgac gtcgcgacgg
    38701 tggccccagg aagagtcgaa gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct
    38761 tctttcatct ctttgatggt ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt
    38821 ttggtgatac cgcagttggc gtagatgtct gcaattttac cgacagaaac cacctggccg
    38881 tgtttttcat caaccagttt ctgcagcacg gtcggtgctg gcggctcaac agccaggtcg
    38941 tgacggttac cggtacgctg gaagttacca gctttgtcac cgataaacgg acgagcgata
    39001 acacgaccga tattgtagcc gccgttggtc agctcttcac gggcgatttc gcacagttcg
    39061 tagagtttat ccagaccgaa agtttcttca tggcaggcaa tctggaacac ggagtcagcg
    39121 gaggtataga aaatcggctt gccggttttc atgtgctctt cgcccagttg atccagaatg
    39181 accgtaccgg aagagtggca gttaccgagg taacccggca gattagcgcg ttcgaccagt
    39241 ttatccagca gctcttgcgg gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac
    39301 agaaccggga caccggcgat ttcccagtga ccagacgggg tatctttacc ggatgacatt
    39361 tcgtgcgccc atgcgtacgc gccgataact tcagcgttgc cgtccattcc cgccggaatg
    39421 aaaccggtag aaccttcgtg tgctttcgcc agccccagac ggatcagatt tggcagattg
    39481 agcggccctt tacgaccatt atcagcttcg cctttggcac aagcttctgc gatatgaccc
    39541 agggtgtcag ccccgacgtc accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat
    39601 gagtccagca ccataataaa tgcacgtttc atatgttctc cgtactcagt gcttcaaatt
    39661 taaatgatca gatcagtata ccgttattcg ctgatacggc gatagacagt tggtgtgctt
    39721 tccggtgcgt tatcggcaag tttaattgcc gctttcaccg ctttcgccgc ttcctgccag
    39781 ctgttttcgt ctttcgcgag gataaccgcc agcggacgct gaccgtctac ctggtcgccc
    39841 agacgcgcca tatcagtaaa gccgacgctg taatcgatgg tgtcagatgc ctgacgacga
    39901 ccgccgccca ttgcaaccac tgccatcccc agcgcgcggg tatccatttc actgacaaaa
    39961 ccttcggtat cagcatagac tgctttcgtc agcatcgctg tcggcagata cttcgcgtag
    40021 ttctcaacga agtcggtcgg gcctttttgt gccgctacca tacgaccaaa gacttctgcc
    40081 gctttaccgt tgtccagcac cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc
    40141 agtttgccgg agatcagcat ctccacgcac agcaccatcg tgacatcaaa cagacgcggg
    40201 ttacgatact cacccgtcag gaactgcacc gcttcacgaa cttcaaccgc gttacctgca
    40261 ctggaggcca gtacctgatt catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg
    40321 ttagccacgc caacaatcgc ttcggcaagg gcttcagaga gttcgtaggt cggcataaac
    40381 gcgccgctac ccactttcac gtccatcacc agcgcgtcca gaccttccgc cagtttcttc
    40441 gccaggatcg aggcagtgat cagcgggatg gagtccacgg ttgcggtaat atcacgggtc
    40501 gcgtagaaac gtttatcagc cggagccagt gaactggtct gaccgataat cgccacgccg
    40561 acgtctttaa taatttcgcg gaaacggttg tcatccggga aaatgtcgaa gccagggatg
    40621 gattccagtt tgtcgagcgt accgccagta tgaccgaggc cgcgaccgga gatcatcgga
    40681 atatagccgc cgcaggctgc gaccatcggc cccaacatca gcgaagtgac atcgccgacg
    40741 ccgccggtcg agtgtttatc aacaatcggg ccatccagat gcaggctttt ccagtcgaga
    40801 acggttcctg aatctcgcat cgccatggtc agcgagacac gctcaggcat tgtcatatcg
    40861 tggaagaaaa tggtcatcgc gagggcggca atctgccctt cggaaatagt gttgtcgcga
    40921 ataccgttga taaagaaacg aatttcttca tcgctcagcg catgaccatc acgtttttta
    40981 cgaataattt cttgtgcgag aaacaaggta acctcccgag gaaaagagta tgaggcggct
    41041 tttgccggat gcgtctacgc cttatccggc ctacgggtag atacgtctcg taggcctgat
    41101 aagcgcagcg catcaggcgt aaagcatctt acttagtagc tgctggcgct cttaccgtcg
    41161 ccgtggccca gcgctttcag caggcttgcc agcaggctgg aagcaccaaa gcggtagtga
    41221 cgcgcatcag cccagtcagc accgaacagt tcatctgcga tggcgagata tttctgcgca
    41281 tcttccgcag tacgcacgcc gcccgccggt ttgaaaccaa cggttttttc tacgcccata
    41341 tcacggatca cttccatcat gatgcgagcg ctttccggcg tcgcgttcac agccacttta
    41401 ccggtagagg ttttgatgaa gtccgcaccc gctttgatgg agatttcaga cgctttacgg
    41461 atcagcgctt cgtctttcag ttcgccggtt tcgatgatca ctttcagcag tacattcgct
    41521 gccgcgcaag cctctttaca ggctttcacc aggtcaaaac caacctgctc gtcacccgcc
    41581 atcagcgcgc ggtacgggaa taccacgtca acttcatcag caccgtaggc gattgccgca
    41641 cgggtttctg ccagcgcgat ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta
    41701 gcgatacgga tttccggggt gccctgctct ttcagagttt tgcgagcaat cgggataaaa
    41761 cgaggataga tacagatagc ggcggtattg ccgaccggag ttttggcctg atgacacaga
    41821 gcaattactt tctcgtcggt gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt
    41881 gcacgcaggc tgcttgcttt cagatcagtc atttcattct cctggcttgt cgccaataaa
    41941 attcaccttg cgagtttgtt agtattctaa catctactcc gcaacacact tcgatacaca
    42001 tcacaattaa ggaaatctac ttacaagttt gcatcactgt aatgcgatct ggttcaaata
    42061 attcactttc aaatgaatgc gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca
    42121 cgaaacttct tacaatggcg catcaccttc ggcataaaag gaaaagaaga tgcccacttc
    42181 tcatgaaaat gcactgcaac aacgttgcca gcaaattgtc accagcccag tgcttagccc
    42241 ggagcagaag cgccattttc tggcactgga agcagaaaac aatctgcctt acccacagct
    42301 tcctgccgaa gcccgccgcg cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca
    42361 tgcgccgtac aaaccgcgct atgtcttacc cgattacgct cgttttctgg cgaacggttc
    42421 cgaatggctg gagctggaag gcgcgaaaga tcttgatgac gcactctctc tgctgaccat
    42481 tctttaccac cacgtaccgt cggtcacatc gatgccggtc tacctggggc aactggatgc
    42541 gttgttgcaa ccgtatgtta gaattctaac acaaaacgag atcgatgttc gaataaaacg
    42601 tttctggcgt tacctcgaca gaaccctgcc agatgccttt atgcacgcca atatcggccc
    42661 gtctgattcg cccattaccc gcgcgatctt acgtgcagac gcgcaggttt caccgaacct
    42721 gacctttatc tacgatcctg aaatcacccc tgatgacctg ctgctggaag tggcgaagaa
    42781 catctgtgaa tgtagcaaac cgcacatcgc caacggtccg gtgcatgata aaattttcac
    42841 aaaagggggc tacgggattg tgagctgtta caactcactg ccgctggcgg gtggtggcag
    42901 cacgctggta cgccttaacc tgaaagccat tgccgagcgc agcgaatcgc tggatgactt
    42961 ctttacgcgc actctaccgc actactgcca gcagcagatc gccatcatcg atacgcggtg
    43021 tgaattcctc tatcaacaat cacacttctt tgagaatagc ttcctggtga aagaagggct
    43081 gattaaccct gaacgttttg tgccaatgtt tggcatgtat gggctggcgg aagcggttaa
    43141 cttgctgtgt gagaaagaag ggattgccgc gcgctacggt aaagaagccg ccgcaaatga
    43201 agtgggttat cgcatcagcg cgcaactggc ggagtttgtc gccaataccc ccgtgaaata
    43261 tggctggcaa aaacgcgcca tgttacacgc acagtcgggg atcagttccg atatcggcac
    43321 cacgccgggc gcgcgtttgc cgtatggcga tgagccagat ccgatcaccc atctgcaaac
    43381 tgtcgccccg catcatgctt attattattc cggcatcagc gacattctga cgctcgacga
    43441 aaccatcaaa cgtaacccgc aggcactggt acagctttgc ctcggtgcct ttaaagccgg
    43501 aatgcgtgaa tttaccgcca atgtcagcgg taacgatctg gttcgcgtta ccggttatat
    43561 ggtgcgtttg tcggatttag aaaaatatcg cgccgaaggt tcacgcacca acaccacctg
    43621 gctgggcgaa gaagccgcac gcaacactcg tattctggaa cgccagccgc gcgtgataag
    43681 ccatgaacag cagatgcgct ttagtcagta agattatccc cttctcctgc gttgacgggc
    43741 caggcagtcg tctggctctg tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca
    43801 atccgtggac gatgggacgt tgcaatgact gtggggagtg cgtgccacag tgtccgcatc
    43861 aggcgttgca gattgttgac ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg
    43921 atacctgcct gaagatgtgt ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg
    43981 acgaagtgct tagccatgtg cgcaaagcgg tgctgtttat tgaagggata acggtaagcg
    44041 gcggcgaagt cacgacccaa ctgccgtttg tagtggcgct gtttactgct atcaaaaacg
    44101 atccgcaact gcgccatctc acctgtctgg tggacagtaa cggtatgttg agcgaaaccg
    44161 gctgggaaaa attactcccg gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta
    44221 gcgaatgtca tcaacaatta accggacgcg ataatcaaca gattaagcgc agcatctatt
    44281 tgctggcaga gcgcggcaag ctggcggaac tgcgtttgct gatgatccca ggccaggtgg
    44341 attatttgca acacatcgaa gaactggcgg cgtttatcaa gggacttgga gatgttccgg
    44401 ttcgcctgaa cgcgtttcat gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg
    44461 ccacgccgga agacgttgag ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt
    44521 tgatatttcc ggcgctctat ttgtgacatg ccggatacgg cctacggttc ggcacagact
    44581 tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg
    44641 cggcgtgaac gccttatccg gcctacggtt cggcacaaac ctgtaggcct gataagacgc
    44701 gttaagcgtc gcatcaggca ttatgcgccg actgccggat gctgcctacg aagggcaaac
    44761 tacccacgaa cgttaaacac cgcatacgtg ttattaagca acacttcggc aatctcatcc
    44821 gcgggttccg ggcgtaactc gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg
    44881 ttaggctgcc cctgaaaacc gttgagcggc atatccggcg catcggtttc cagcaacaac
    44941 gatgccagcg gtaattttgc gatgacatcg cgggttttac tggcgcgtgg ataggtgata
    45001 gtaccgccta cgccgatttt gtagcccagc tgtacaaacc gttcagcctg ttgctggctg
    45061 ccagaaaatc cgtggacaac accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc
    45121 agtttgtcgt gcgtgcgccg tgaatgcagg atcaccggca gatcgtagcg tttcgccagt
    45181 ttcagttgtt cgtcgagtaa ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga
    45241 tccagaccga tctcccccac cgccaccacc ttcgccggac gcctttccag cgcctgctgt
    45301 agctgctcaa gagacacatc gctatgtttt tccaacatac caggatgcaa gcccaatgcg
    45361 gcatacagcg gttgataatt ttccgctaac gcctgcacgc gggcaaaatt ttccgcctct
    45421 gtcgccggaa caatgatctt gcctacgccc gcttgtgccg cgcgttgcag gctggcctct
    45481 tcatcgccaa taaaaggcgg gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc
    45541 aagccagatc ctcattgtta aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg
    45601 caaccagcgt atcgttggcg actggcgcag gcggaatgac aatcgattcc ggcgtcacca
    45661 ctggcaccag atggcgggta agcggcgctt tttcagttaa tagcttgcca accgtggcga
    45721 ggaaataacg accgcataaa cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac
    45781 tgccaagcgc gatactatgt aatggcttcg gcggataaat ttcggatatc cgcagcttgc
    45841 ccggtggttt ctcaataaat tgctgaatgt cacgatagct ggtttcatga tgctgcacca
    45901 gattgaccag cggctgcagg ctactgtcac ccagccagcg ttccatgcgt ttgaaccact
    45961 gcggcgtgta gtacatttgt gacggcacag tgcggatgac gaccaacgtt ttagcgccct
    46021 gccttgccgc ctctttaacc ggaatcgcat cactgatccc gccatccagg tagttaatgc
    46081 cttccagcga cactccgcta cgataaaagc caggtatcgc actggaggcg cgaatcacat
    46141 ccagccagtt ttgtttggtt ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg
    46201 cgcacatata aaacgatttg ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg
    46261 gcatctggct tgcggtggcc tccaccagcc agtcgagatc gataagattt cctccacgga
    46321 caaagcgcaa tggatcaaaa aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg
    46381 cgtaaccggg ctgattgcag ataaacgccg agaggttctg cgccccggca gatgtgccga
    46441 gataaagatc gaaaggatta aactgcgcgc gcataaactc atccagcacg ccagccgtga
    46501 aaattccacg ctgtccgccg ccttcgcaca ccagagctat tcgtccaggc tggaacggcc
    46561 ttagcgacaa cggcgcaata ttaccaagcg ttacaggtat tctctgcccc acttctgcct
    46621 tcctgttttt gttgtgaaag aaagacaaag taacgcaatt tattattcgc taaaaccgca
    46681 aaagccagtc cgctggactg gctttggata aaattttctt aatggtctga aatctagggt
    46741 cgttttcggc ccatgaacaa actcactagg aacagaataa tcccgacgac aaagacaatt
    46801 ttagctgcgc ctgcagcggt accggccaga ccaccaaacc caagtgcggc ggcgattaac
    46861 gcgataacca gaaatatgat gccccaacga aacataagcg tctcctttac catagttaat
    46921 gtcaccgctt gatatgagcg tgaaaatcac tcaatgcgat atttttagtg tggtgcacat
    46981 cgcgcctccc gacaaagttc gggaggacga attacgacga attacttagt tttcagatca
    47041 tttttaacgc ttttcacacc atctaccgct ttggcgatac tttcagcacg gtcactttgt
    47101 gcctgagaat ctacggtacc ggagagctga accacgccat cggtggtttc aactttcact
    47161 ttacgtgaag ggacgatatc gtccgccagc agtttggctt tgatttcact ggtggtggcg
    47221 gtgtcacccg cgtagtcctt caccgagcct tctttagcgt cgcgaacgtg cagtttgtcg
    47281 ctgacagagg tcacgccttc aacgcctttc gccactttca ctgcctcttc ggcctgggcc
    47341 tgactttcaa cgaaaccgct cagggtcacg actttttgat cggttttgac agagatatcg
    47401 gtgctcttga tgttgtcatg atccaccagc gccgccttca ctttcgcggt gatggcgctg
    47461 tcatccatga aattaccgac tttattcata gagctatcga ctttttgccc tgcgctttca
    47521 ttggtagtct gcgcgttgtt ttccgcgtag gcagagccgg tcgcgacggc agaggtcaac
    47581 attacagcca gcagagtttt cgaaatcttc agtcttgtca tagtcatcga tttattcctg
    47641 tatgtttgct cgtaatttga gcctagcaac acgaggttgc attgctgaat agggagagac
    47701 ttcaccctct acagaagtca atggtcgcca tcacaaaagc gatgggtgat gaataacgac
    47761 cattacagtc tctgaatcag ttattaatat cggtagaatg acaatcgacg gctttagata
    47821 ctgatatcta cgcattgaac ggtatttaac gccatcagaa atgtcatcac tttgttaaat
    47881 atagatcaca attttgaaac cgctcgggat atcagcgaaa acataagcaa aagtgaatgt
    47941 tttaagaaca ttccgtaagc ggctaataag gaagggaaat tgacagggcg cagcggctac
    48001 cgcgccctgg aggcaagaat taatgctcgc gggtctggtg gaactgaacg tccggataac
    48061 gttcctgtgc caggcgcagg ttgaccatgc tggtagcgat gtaagcgagg ttatcgccgc
    48121 catcaagcgc cagttggctt tcgttcttac gcttgaactc ttcgaatttc ttcgcgtctg
    48181 cacattctac ccagcgggca gtggcaacgt tgactgactc atacactgct tcaacgttgt
    48241 attcgctctt caggcgcgct accaccacat caaactgcag cacaccaacc gcaccaacga
    48301 tcagatcgtt gttggagatt ggacggaaca cctgcaccgc gccctcttcg gaaagctgta
    48361 ccagcccttt gagcagctgt ttttgcttca gcggatcttt caggcggata cgacggaaca
    48421 gttctggtgc gaagttcgga ataccggtga acttcatcat ctcaccctgg gtaaaggtgt
    48481 cgccgatctg aatggtgccg tggttgtgca ggccgaggat atcgcccgga tacgcttctt
    48541 caacgtgcga acggtcaccc gccataaagg tcagcgcgtc ggagatcacc acatctttcg
    48601 cagtgcgcac ctggcgcagt ttcatgcctt tttcatattt accggacacc acacgcataa
    48661 acgccacgcg gtcgcggtgt ttcgggtcca tgttggcctg aattttaaat acgaagccgg
    48721 taaatttatc ttcgctcgct tctacggtac gggtatcggt ctgacgcggc atcggcgcag
    48781 gcgcccactc caccaggcca ttcagcatat gatcaacgcc gaagttaccc agcgcagtac
    48841 cgaagaatac cggagtgatt tcgcccgcaa ggaacagctc tttgtcgaac tcgttagacg
    48901 cgcctttaac cagttccagt tcgtcacgca gctgctgtgc cagatcttca ccaaccgcag
    48961 catcgagatc cgggttattc agccctttaa caatgcggac ttcctgaatg gtgtgccctt
    49021 taccgctctg atagagatag gtttcatctt tataaaggtg gtaaacgcct ttaaacagct
    49081 tgccgcagcc aattggccag gtgatcggtg cgcagccaat tttcagctcg ttctcaactt
    49141 catcgagcag ttccatcggg tcgcggatat cacggtcaag tttgttcata aaggtgagga
    49201 tcggcgtgtc gcgcagacgg gtaacttcca tcagcttacg ggtacgatct tcaacacctt
    49261 ttgcggcgtc gataaccatc aggcagcagt ccaccgccgt cagggtacga taggtatctt
    49321 ccgagaagtc ttcgtgcccc ggggtgtcga gcaggttaac caggcaatcg tgatacggaa
    49381 actgcatcac agacgtagta atggagatcc cacgctgctt ttccatctcc atccagtccg
    49441 acttagcgtg ctggttggaa ccacggcctt ttactgtacc ggcggtctga atggcctgtc
    49501 cgaacagcag caccttctcg gtgatggtag ttttaccggc gtccgggtga gaaataatgg
    49561 caaaagtgcg gcgcttcgcc acctcttgca aataaggaga caacgtcata attctttctt
    49621 cttgagtaag cggcatcgcg ccgcgcttgt tgaaaacgaa aaattgcggc tattttaccc
    49681 atcaacgggg gggaggcaat cagtgtttac acaggagctg ctccagttcg tgcaacgaag
    49741 aaacggtcca ggtgggcgcg atgccttctg gttgctcgcg attgtgcgca ttcagccagc
    49801 aggtcgcaag cccggcgttg atgccaccga gaatatcgga ctcggcagtg tcgccaacca
    49861 tcagcacgcg tgaacggtca ggattgcccg cctgttccag cgcataatcg aaaattttct
    49921 tattcggttt ggcaacgcca acttcttcgg aaatcaccag caaatcgaag taatcacgca
    49981 ggcccgtccg ttccagacgc acttgctgca aggcgctaaa accgttggtg atgatgccga
    50041 ttttagcgtt gccacgaatg gcgttaagca gagaaaccgc gcccggcaac ggcgtacaga
    50101 tttccgccat cgcattaata aaggcatcgt taagcaagcc tgcttcaact ttcagccgtt
    50161 cggcccagct ctcgaaacgc ccgtgctgaa gctgtaatga agtgatcgcg ccgttttgat
    50221 aatccaccca cagtggcttg ttaacggcct gatagtcctg aaaatcttca gcggtaaagg
    50281 tgacgctgta atcaagaaac atccgctgca ggccggtgaa tgagtcaaag gtaaacagcg
    50341 tttcatcggc atcaaagaaa atccagtccc acttcattat tccaccttgt cttacatact
    50401 gattggcaac gccatgatga tggcgtcttc gcgaccgtcc gtggtggggt agtaattgcg
    50461 gcgaatcgtc gcctcgttaa agcctaaact ttcgtacagg gcaatggcgg cagcatttga
    50521 ggcacggact tccagccata gtgtcgccac gccgcgtttt tccagttcgt cgatcagatg
    50581 ttccagcagc gccctaccca gcccctgacg ctgatagtcg ggatcgaccg caatattgaa
    50641 caatgtagct tcatccagca ccacttgcgt aatcgcaaac gccgccattt tgccgttttg
    50701 cgttaactga aagttgagat aacgctcgcc ctggttgctg gcaaaggttt tttcactcca
    50761 cggaaaggcg tgggcgcgtt gttcaatgtg gtaagccgct ggtaaatcag tcgtttcgag
    50821 ggaagaaatc gtgttcatat gtgcaaattt gttgccataa cgcggcgcgt gccgttgggt
    50881 ttgcccgtaa ttcggtgagc gccggtgatg ccacctgagc gccttccaat gatagcggtt
    50941 cgtcagtacc caaccgccag ctgttgcagc gactgccttg cggcagcatc gcgatttttt
    51001 ctggcgtcag ttgcagcact tgatcggggc tgacggttaa tgcgcgcaga acatcgctca
    51061 ctaaaggatc agtcagggcg ggaagatcgt ttgccaccat caccagacgg acgtgtgccg
    51121 ggctcgcaat ggcaatctcc ccctgcaacg cgccagggcg acgcagcgac cactgggtaa
    51181 tgcccagttg ctgtaactgc cagtctcgtc gggatgtcat agcgaatcgc tcctgttgtc
    51241 aggggcgcaa atatagcaaa ttcgtcgata ccgcgccaac atatggctat aatcgccgcc
    51301 agtatcaatt gaggagcatt ccatgtctgc atttaccccg gcaagtgaag tcttgctgcg
    51361 tcacagtgat gatttcgaac aaagccgtat tctgtttgcc ggagacttac aggatgacct
    51421 gcccgcgcgt ttagataccg cggccagccg tgctcatacc cagcaattcc accattggca
    51481 ggtgttaagc tgccagatgg gggataacgc ccgttttagt ctggtcgcca cggcgaatga
    51541 tgtcgcagat tgcgatacgc tgatttacta ctggccgaag aacaaaccgg aagcccagtt
    51601 ccagttgatg aatttacttt ctctgctgcc agtggggaca gatatttttg tcgttggcga
    51661 gaaccgcagc ggcgtgcgca gcgctgagca gatgctggca gattatgcgc cgttgaataa
    51721 agtcgacagc gctcgtcgct gtggcctcta ttttggtcgt ctggaaaaac agccagtatt
    51781 tgatgcggat aaattctggg gcgaatacag cgtcgatggc ctgacggtca aaacgctgcc
    51841 tggcgtgttt agccgcgacg gtctggatgt cggtagccag ttgctgctct cgacgttaac
    51901 tccgcacacg aaaggtaaag tgctggatgt cggctgtggc gcgggtgtac tttcagttgc
    51961 ctttgcgcgc cattcgccga aaattcgtct caccttgtgc gatgtctctg ccccggcggt
    52021 agaagccagc cgcgcaacac ttgcagccaa cggtgttgaa ggtgaagtct ttgccagcaa
    52081 cgtcttttcc gaggtgaaag gttgttttga tatgatcatc tccaacccgc cgttccacga
    52141 tgggatgcaa accagcctgg atgcggcgca aacgctgatt cgtggcgcgg tgcgtcatct
    52201 taatagcggc ggcgagctgc gaattgtagc gaacgccttc ctgccttacc cggacgtgct
    52261 ggatgagaca tttggcttcc atgaagttat cacgcaaacc gggcgcttca aggtatatcg
    52321 cgccattatg acccgccagg cgaagaaagg ttgagtatct gatatctgtc gccgcatacc
    52381 gtgtcggatg cggcgtgaac gccttatccg acctacaaaa cctgcacgtt agcccttcgt
    52441 aggccagata agacgcgcca gcgtcgcatc tggcatctgc ccccgcatgc ctaccaaacc
    52501 tgcacgctag tttcctgatg gacatttttc cagcaattac acatcaggcg ataattaact
    52561 attgacgaaa agctgaaaac cactagaatg cgcctccgtg gtagcaattc tttttaagaa
    52621 ttgatggtat gcgaaggtgg cggaattggt agacgcgcta gcttcaggtg ttagtgtcct
    52681 tacggacgtg ggggttcaag tccccccctt cgcaccaaaa accacgttga tattgctcgc
    52741 actgggcg
//
LOCUS       DAJTYF010000015        50317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTYF010000015 DAJTYF010000000
VERSION     DAJTYF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.015"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..362
                     /locus_tag="OO590_000988"
     CDS             <1..362
                     /locus_tag="OO590_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6636452.1"
                     /translation="YMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIF
                     LGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLA
                     IGMLGIAMICLLFIKDI"
     gene            473..1396
                     /locus_tag="OO590_000989"
     CDS             473..1396
                     /locus_tag="OO590_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6636453.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1400..2218)
                     /gene="nlpA"
                     /locus_tag="OO590_000990"
     CDS             complement(1400..2218)
                     /gene="nlpA"
                     /locus_tag="OO590_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR6636454.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2440..2733
                     /locus_tag="OO590_000991"
     CDS             2440..2733
                     /locus_tag="OO590_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636455.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2774..3964)
                     /gene="nepI"
                     /locus_tag="OO590_000992"
     CDS             complement(2774..3964)
                     /gene="nepI"
                     /locus_tag="OO590_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6636456.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4175..4627)
                     /locus_tag="OO590_000993"
     CDS             complement(4175..4627)
                     /locus_tag="OO590_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6636457.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4680..6014)
                     /gene="adeQ"
                     /locus_tag="OO590_000994"
                     /gene_synonym="yicO"
     CDS             complement(4680..6014)
                     /gene="adeQ"
                     /locus_tag="OO590_000994"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6636458.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6189..7955
                     /gene="adeD"
                     /locus_tag="OO590_000995"
     CDS             6189..7955
                     /gene="adeD"
                     /locus_tag="OO590_000995"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6636459.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPAVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8001..9392)
                     /gene="uhpT"
                     /locus_tag="OO590_000996"
     CDS             complement(8001..9392)
                     /gene="uhpT"
                     /locus_tag="OO590_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6636460.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9530..10849)
                     /gene="uhpC"
                     /locus_tag="OO590_000997"
     CDS             complement(9530..10849)
                     /gene="uhpC"
                     /locus_tag="OO590_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6636461.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(10859..12361)
                     /gene="uhpB"
                     /locus_tag="OO590_000998"
     CDS             complement(10859..12361)
                     /gene="uhpB"
                     /locus_tag="OO590_000998"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6636462.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12361..12951)
                     /gene="uhpA"
                     /locus_tag="OO590_000999"
     CDS             complement(12361..12951)
                     /gene="uhpA"
                     /locus_tag="OO590_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6636463.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13026..13316)
                     /gene="ilvN"
                     /locus_tag="OO590_001000"
     CDS             complement(13026..13316)
                     /gene="ilvN"
                     /locus_tag="OO590_001000"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6636464.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13320..15008)
                     /gene="ilvB"
                     /locus_tag="OO590_001001"
     CDS             complement(13320..15008)
                     /gene="ilvB"
                     /locus_tag="OO590_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6636465.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(15114..15212)
                     /gene="ivbL"
                     /locus_tag="OO590_001002"
     CDS             complement(15114..15212)
                     /gene="ivbL"
                     /locus_tag="OO590_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6636466.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15777..15866
                     /gene="tisB"
                     /locus_tag="OO590_001003"
     CDS             15777..15866
                     /gene="tisB"
                     /locus_tag="OO590_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6636467.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16146..17330
                     /gene="emrD"
                     /locus_tag="OO590_001004"
     CDS             16146..17330
                     /gene="emrD"
                     /locus_tag="OO590_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6636468.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17338..17835)
                     /locus_tag="OO590_001005"
     CDS             complement(17338..17835)
                     /locus_tag="OO590_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6636469.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17832..18194)
                     /locus_tag="OO590_001006"
     CDS             complement(17832..18194)
                     /locus_tag="OO590_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6636470.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18184..18531)
                     /locus_tag="OO590_001007"
     CDS             complement(18184..18531)
                     /locus_tag="OO590_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6636471.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLTWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18640..19053
                     /locus_tag="OO590_001008"
     CDS             18640..19053
                     /locus_tag="OO590_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636472.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19100..20483)
                     /locus_tag="OO590_001009"
                     /pseudo
     CDS             complement(19100..20483)
                     /locus_tag="OO590_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(20518..22233)
                     /locus_tag="OO590_001010"
                     /pseudo
     CDS             complement(20518..22233)
                     /locus_tag="OO590_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22400..23293
                     /locus_tag="OO590_001011"
     CDS             22400..23293
                     /locus_tag="OO590_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6636473.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23290..24104)
                     /locus_tag="OO590_001012"
                     /pseudo
     CDS             complement(23290..24104)
                     /locus_tag="OO590_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24104..25720)
                     /locus_tag="OO590_001013"
                     /pseudo
     CDS             complement(24104..25720)
                     /locus_tag="OO590_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            26015..26689
                     /locus_tag="OO590_001014"
     CDS             26015..26689
                     /locus_tag="OO590_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6636474.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(26728..28389)
                     /locus_tag="OO590_001015"
     CDS             complement(26728..28389)
                     /locus_tag="OO590_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6636475.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(28585..29013)
                     /gene="ibpB"
                     /locus_tag="OO590_001016"
     CDS             complement(28585..29013)
                     /gene="ibpB"
                     /locus_tag="OO590_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6636476.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(29125..29538)
                     /gene="ibpA"
                     /locus_tag="OO590_001017"
     CDS             complement(29125..29538)
                     /gene="ibpA"
                     /locus_tag="OO590_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6636477.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(29532..29604)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            29843..30175
                     /locus_tag="OO590_001018"
     CDS             29843..30175
                     /locus_tag="OO590_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6636478.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGSKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(30177..31391)
                     /locus_tag="OO590_001019"
     CDS             complement(30177..31391)
                     /locus_tag="OO590_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6636479.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            31492..32510
                     /locus_tag="OO590_001020"
                     /pseudo
     CDS             31492..32510
                     /locus_tag="OO590_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(32471..33808)
                     /locus_tag="OO590_001021"
     CDS             complement(32471..33808)
                     /locus_tag="OO590_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6636480.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(33883..35031)
                     /gene="dgoD"
                     /locus_tag="OO590_001022"
                     /pseudo
     CDS             complement(33883..35031)
                     /gene="dgoD"
                     /locus_tag="OO590_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(35028..35645)
                     /gene="dgoA"
                     /locus_tag="OO590_001023"
     CDS             complement(35028..35645)
                     /gene="dgoA"
                     /locus_tag="OO590_001023"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6636481.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKVVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(35629..36507)
                     /gene="dgoK"
                     /locus_tag="OO590_001024"
     CDS             complement(35629..36507)
                     /gene="dgoK"
                     /locus_tag="OO590_001024"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6636482.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLLR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(36504..37193)
                     /gene="dgoR"
                     /locus_tag="OO590_001025"
     CDS             complement(36504..37193)
                     /gene="dgoR"
                     /locus_tag="OO590_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6636483.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            37471..38127
                     /locus_tag="OO590_001026"
     CDS             37471..38127
                     /locus_tag="OO590_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636484.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(38173..38985)
                     /gene="yidA"
                     /locus_tag="OO590_001027"
     CDS             complement(38173..38985)
                     /gene="yidA"
                     /locus_tag="OO590_001027"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6636485.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(39099..39497)
                     /locus_tag="OO590_001028"
     CDS             complement(39099..39497)
                     /locus_tag="OO590_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6636486.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(39737..42151)
                     /gene="gyrB"
                     /locus_tag="OO590_001029"
     CDS             complement(39737..42151)
                     /gene="gyrB"
                     /locus_tag="OO590_001029"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6636487.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(42180..43253)
                     /gene="recF"
                     /locus_tag="OO590_001030"
     CDS             complement(42180..43253)
                     /gene="recF"
                     /locus_tag="OO590_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6636488.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPRFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(43253..44353)
                     /gene="dnaN"
                     /locus_tag="OO590_001031"
     CDS             complement(43253..44353)
                     /gene="dnaN"
                     /locus_tag="OO590_001031"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6636489.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(44358..45761)
                     /gene="dnaA"
                     /locus_tag="OO590_001032"
     CDS             complement(44358..45761)
                     /gene="dnaA"
                     /locus_tag="OO590_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6636490.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            46058..46138
                     /locus_tag="OO590_001033"
     CDS             46058..46138
                     /locus_tag="OO590_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636491.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            46368..46508
                     /gene="rpmH"
                     /locus_tag="OO590_001034"
     CDS             46368..46508
                     /gene="rpmH"
                     /locus_tag="OO590_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6636492.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            46525..46884
                     /gene="rnpA"
                     /locus_tag="OO590_001035"
     CDS             46525..46884
                     /gene="rnpA"
                     /locus_tag="OO590_001035"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6636493.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCLLARGS"
     gene            46848..47105
                     /gene="yidD"
                     /locus_tag="OO590_001036"
     CDS             46848..47105
                     /gene="yidD"
                     /locus_tag="OO590_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6636494.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            47108..48754
                     /gene="yidC"
                     /locus_tag="OO590_001037"
     CDS             47108..48754
                     /gene="yidC"
                     /locus_tag="OO590_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6636495.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            48860..50224
                     /gene="mnmE"
                     /locus_tag="OO590_001038"
     CDS             48860..50224
                     /gene="mnmE"
                     /locus_tag="OO590_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6636496.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        12419 a        13531 c        12685 g        11682 t
ORIGIN      
        1 attacatgaa acgtattggc aagcgactat taatgctcat tgctatcgtg agtggtatgt
       61 gtttttacgc cagcgtactc atggcgacga ctccggcggt tgagctggaa ttgcaaattc
      121 taaatgccat cttccttggt attctctgtg gtatcggcat gctttatttt caggacttga
      181 tgcctgaaaa aataggctct gcgacaacgt tatatgcaaa tacttcacgc gtcggctgga
      241 ttatcgccgg ctctgttgac ggaattatgg ttgaaatctg gagctaccat gcgttgttct
      301 ggctggcgat agggatgttg ggtattgcga tgatttgcct gctgtttatt aaagatattt
      361 agctcaccag tctgggctaa gaaaaatcgg cgtggcgcaa acagagccat cctcgccatt
      421 tttgctatca tgcctgcata cataaacgac aaaacagtat gcagagggaa aaatgggttc
      481 caccagaaag gggatgctga acgttctgat tgccgccgtg ttgtggggaa gttcaggggt
      541 ctgtgcgcaa tacatcatgg agcaaagcca gatgtcgtcg cagtttttga ctatgacgcg
      601 tttgatattc gccggtttga ttctgctgac gctgtcattt gttcatggcg ataaaatctt
      661 ttctattatt aacaatcaca aagatgccat tagtctgctg attttttccg tggttggcgc
      721 gctaaccgta cagctcactt ttttgctaac catcgaaaaa tcgaacgcag ccacggcaac
      781 ggtgctgcaa ttcctctcac cgacgattat cgtcgcctgg ttctcactgg tacgtaaatc
      841 gcgcccgggc attctggttt tctgcgctat tttgacatcg ctggtcggga cttttttatt
      901 ggtgacacac ggtaatccga cgtcattatc gatctctcct gccgcgttat tctggggcat
      961 tgcctcggca tttgctgctg cattctatac cacctatccc tcaacgctga ttgcccgcta
     1021 tggcacgttg cctgtcgtcg gctggagtat gctgatcggc ggtctgattc tattgccttt
     1081 ttatgccaga caagggacaa actttgtcgt taacggcagt ttgattttgg cgttttttta
     1141 tttgatcgtc attggtacgt ccctgacatt tagtctgtac ctgaaaggag cacaattaat
     1201 tggcggtcca aaagccagca ttttgagctg tgcagaacca ttaagtagcg cgctactctc
     1261 tttgctgttg ctggggatca cgttcacatt accggactgg ctgggaacgc tgctgattct
     1321 gtcatcggta attttgattt caatggattc ccgtcgccgc gccagaaaaa taaatcgtcc
     1381 ggcgcgggat gagtgagaat taccagccag gcaccgcgcc accgttaaag atggtttctg
     1441 ccgctttagc gacttcggat gattgataag attgcagaaa ttccttcacg ttttctgcat
     1501 tcttattatc ttcccgtgcc accaaaatat tcacatacgg cgaattttta tcttcaataa
     1561 atacactgtc gtgcaccgga gaaagcccgg tctgctgaat gtaagtggtg ctgataatcg
     1621 ccacatcaac tttgggatca tccagtacgc gcgggagctg cgccccctcg agttccataa
     1681 tctgcaaatg acgaggatta tcggttatat ccagcgcggt aggtaataac ccttttccct
     1741 ctttcagagt aattagcttc tctttttgca acagtaaaag cgcacgacca aggttggtcg
     1801 ggtcgttggg gatcgccact gtcgcgcctt cttttatttg cgccacggtt ttgatttttt
     1861 tggaataacc cgccatgggg aagacaaacg tatttcccac cgctaccagt ttataaccat
     1921 gcgcctgatt atcttgttca agaaagggtc gatgctggaa gacgttcgca tcaagttcgc
     1981 catgatttgt cgcatcgttc ggtagcaacg aaccgctaaa tcctaccagt tgaacatcga
     2041 ggccatattt ctcttttgcc acctttttag cgacttccgc gacatcttgt tctgcgccat
     2101 ttataacgcc aactttaatg tgttttgcat cgctgctact ctggtcgcaa cctgccagca
     2161 gaattccggc cagcaataat gcggcccctg tccgtagatg atgtgttgtc agtttcattt
     2221 ttttatcctt ttattaatga ttacctgact atagcggtaa ggtcgctgcg gtttaaaaaa
     2281 cgaaacgcta tcgataagaa taaaaaggaa taaaagtgga atataaagtc tggcacttct
     2341 tactgaccac gcaagcgcgt ttcgtacagc atgatgagag cgatgaaagc aaactacact
     2401 tatgcttcat tcgatacaca tttgtaaaag gttgaatcca tgaaaccaac gacactactg
     2461 cttattttca cttttttcgc tatgccaggc atcgtatacg ccgagtcgcc ttttagctca
     2521 ctgcagtccg caaaagagaa aaccacggta ttacaagatt tacgtaaaat ttgcacgcca
     2581 caggcgtcat tatcagatga agcgtgggaa aagttaatgt tgtcagatga aaacaataaa
     2641 caacatatac gcgaggccat cgtggcgatg gaacgtaata atcagagcaa ttattgggaa
     2701 gcgttgggta aggtagaatg cccggatatg taagaacagc aacaactact gaccaacgct
     2761 tctttacctg atatcaggat ttcttcattt tcacctttgc agtaaccaac aatgcagtca
     2821 gcaacatcaa tgtgccggac aacatcaacg gcgaagtcag accaatatta tccagcgcat
     2881 aaccgccgat tgccgcgcca caggtattag caagctgaat aaccgccacc tgaatagacc
     2941 cggctttttc tgcctgatcg gccagcgagc gggtgatcca cgttgaccag ccgacgggaa
     3001 ccaatgcaaa agttagcccc cagataatcg ccacgccggt agcaacgatt ttatcgcttc
     3061 cccacaacgt cagtaccaac gcactcacag ccagtattaa cggcgcgcct gctaaggcca
     3121 gttttaccga acgtttaaga atgaacgacg aaagcgacgt accaataaag ctggcgatac
     3181 caaaactcaa cagcaccagc gttaagccat ccacgccgaa tcccgccagg ttcatataca
     3241 ccgggcgaat atacgtgaag aaagcaaact gcccggcgaa agacatgaag atggcgatca
     3301 tccctgccat cacacccgga cgttgtaata agcggaaagt attttgtttc tgatgcgagg
     3361 gttcgcctgg cagtgaaggc aatgatttga taatccagaa aatacacagc acgcccatca
     3421 ccgccgccgc attaaagaca ttgcgccaac cgataagctc gcctaaaaaa ctgcccaacg
     3481 gcgcggcaat caccagcgca atagaaaccg cgccgaagat caccgacagc gccttcggca
     3541 ccgtacgcgg cggtaccaga cgcatggtca gcgacgccga catcgcccag aacccgccca
     3601 gcgccagccc cagacaggca cgaccgatta aaagcaaact gaatgagtta gcaaaggaaa
     3661 ccagcaagca ggagagcgtc agcaaaacgg caaacaaaat aacaacgtag cggcggtcag
     3721 tggcctgaat tgtctgggta ataaacaaac tggcaaacat tgccacaaag gcggtcacgg
     3781 tcaccgattg cccggcaacc ccttccgaaa tgcctaaatc ctgggccatt ggcgtcaaca
     3841 aactgacggg caaaaactca acgataatca gacaggcgac acaaaacgcc accgagaaaa
     3901 cggctgacca gttcggtcgg gtgatggcat ccgcgccgcg gttttcggca ataaattcac
     3961 tcatggtgtt acccgtggca tgtttcaggg gaaaagccga ccactttaac attgaaatgt
     4021 gtgacgcatt taacgttttt gcaactttta cggtgttgct tgagctggag ttaagatcga
     4081 aacggaacag ggcaaagatt accggatgcg gcgtgaacgt cttatccacc cgacaggtca
     4141 ggcgcggtct gtaggctgat aagacgaaac ggtttcaatg caaccagtga acgccctcgg
     4201 caggctgaaa gaacgcgtta tagcgcatct gaaaagcatt aatatcctgc atttcaggtt
     4261 cggtttcacg cagaaaaccc agcgccagcc tgacctgagc atcggtaatc ggcgcggaaa
     4321 ggcgggcttt ttgtaaaatc cggtgccatt gcagcaggtt ctgttcgcta tcatcgacaa
     4381 tgacaatttg ccagataagc aacacctgcg cggcgttttg caggtgcgac tcatccgggc
     4441 gatgcaaata acgtaaaaat tcgtcaccgg ctgactttcc catttgatcg accatcgact
     4501 ccacgggtac gacatcgata ttcagccgat cgctgagacg gcgaatcgct tttctggccc
     4561 cggatgtcag cacccgaaaa ccaaccacaa acactaccgc cagcgtggcg agcattatcc
     4621 agatcatgag ttctcctgcg taatcagaac tcatgatatc tggaaaccct cgccagttac
     4681 taatccacca gaataatctt caatgcgaac agagctgcga ccaccaccac acacaggttc
     4741 agatcgcgcc agcgcccggt gcacactttc atgatgcagt acgacataaa gccgagtgca
     4801 atcccttcgg tgatcgagaa agtaaagggc atcatcaccg tggtaataaa cgcaggcacc
     4861 gattcggtaa aatcatccca gttaacgcgc gccaggctcg aagtcatcag cacgccaaca
     4921 aagattaacg ctccagcggt tgcgtaagga ggaaccatcg ccaccagcgg tgagaagaac
     4981 ataactaaca ggaacataac gccgaccacg acagccgtta gccccgtgcg accaccgact
     5041 gccacaccag aagtactttc aatataggca gtaacagacg aggtaccgat aaatgcgcct
     5101 gccaccgaac tgacgctatc aacatacagc gccttattca tattggggaa tttaccgttg
     5161 ctatctatta agcccgcttt atcagttaca ccaattaatg ttcctgatga atcaaatagg
     5221 ttgatcagca taaaggagaa aatgataccg gcgagttcaa gtgttaacgc gccgctcaaa
     5281 tctacttcac caatgacgcc gctaatatca ggcggaatgg aatagacgcc gctaaaatga
     5341 acatcaccga aaaataatcc acagcaagac gtcaccacaa tagaaaccag caccgcggca
     5401 tgaaaatgac gtgatgacaa cacggttata ataaaaaacc ctaaaatacc taacaacacg
     5461 ccgtgagaac ttaaatcgcc aatcatcacc agcgtgtctt tattggcgac aataacacca
     5521 gtgtttttta atcccattaa ggcaataaat aatccgattc cactggtgat accaatacgt
     5581 aaacttaatg gaatgttgga gatcatccag taccggatac gaaacagcgt gagcaaaaat
     5641 agcccaattg cgccccagaa tatcgcgccc atcccggtct gccaggagat ccccatcgcc
     5701 cccacgacca cgaacgcaaa gaaggcgttc agccccattg ccggagccag caccacgggt
     5761 aagttagcaa atatccccat cgcaatactg ccgataccgg caatcaaaca ggtggtaaca
     5821 aacaccactt tcgggtccat ttgtgccgcg ccgaggattt gcgggttcac aaaaacaatg
     5881 tacaccatgg ttaaaaaagt ggtcatcccc gcaatcaatt ctgtgcggac ggtggtccca
     5941 tgctgaggta gtttaaataa tcgcgaaagc gtccctgatt cattactcac gtaatcggta
     6001 ttgtcattat tcattttttt gtccattgga ggagatttaa tcccttgctt ttgtaaaaca
     6061 aatgacaaca acggttcagt gataattatc acatttcaat tgcacattaa tagatattct
     6121 ttaataatct cgcgacgttt ctttatgata aataataatc aaattgataa aatcaaaatg
     6181 agaaaaatat gaataattct attaaccata aatttcatca cattagccgg gctgaatacc
     6241 aggaattgtt agccgtttcc cgtggcgacg ctgttgccga ttatattatt gataatgtct
     6301 ctattctcga cctgatcaat gccggagaaa tttccggccc aattgtgatt aaaggacgtt
     6361 acattgccgg tgttggcgca gaatacgctg atactccggc tttgcagcgg attgatgctc
     6421 gcggcgcaac ggcggtgcca gggtttattg atgctcacct gcatattgaa tccagcatga
     6481 tgacgccggt cacttttgaa accgctaccc tgccgcgcgg cctgacgacc gttatttgcg
     6541 accctcatga aatcgtcaac gtgatgggag aagccggatt cgcctggttt gcccgctgtg
     6601 ccgaacaggc aaggcaaaac cagtacttac aggtcagctc ttgcgtgccc gctctggaag
     6661 gctgcgatgt taacggtgcc agttttaccc ttgaacagat gctcgcctgg cgggaccatc
     6721 cgcaggttac cggccttgca gaaatgatgg actaccctgc cgtaattaac gggcagaatg
     6781 cgctgctcga taaactcgat gcatttcgct acctgacgct ggacggtcac tgtccgggtt
     6841 tgggtggcaa agaacttaac gcctatattg ctgcgggtat tgaaaactgc cacgaaagtt
     6901 atcagctgga agaaggacgc cggaaattac aactcggcat gtcgttgatg atccgcgaag
     6961 ggtccgctgc ccgcaatctc aacgcactgg caccgttgat caacgaattt aacagcccgc
     7021 aatgcatgct ctgtaccgat gaccgtaacc cgtgggagat cgcccatgaa gggcatatcg
     7081 atgccttaat tcgccgcctg atcgaacaac acaatgtgcc gctgcatgtg gcatatcgcg
     7141 tcgccagctg gtcgacggcg cgccactttg gtctgaatca cctcggctta ctggcacccg
     7201 gcaagcaggc cgatatcgtc ctgttgagcg atgcgcgtaa ggtcacggtg cagcaggtac
     7261 tggtgaaagg cgaaccgatt gatgcgcaaa ccttacaggc ggaagagtcg gcgagactgg
     7321 cgctgtctgc cccgccatat ggcaatacca ttgcccgcca gccagtttcc gccagcgact
     7381 ttgccctgca attcacgccc ggaaaacgct atcgggtcat tgacgtcatc cataacgaat
     7441 tgattacgca ctcccgctcc agcgtctaca gcgaaaatgg ttttgatcgc gatgatgtgt
     7501 gctttattgc cgtacttgag cgttacgggc aacggctggc tccggcctgt ggtttgctcg
     7561 gcggctttgg cctgaatgaa ggcgcgctgg ctgcgacagt cagccatgac agccataata
     7621 ttgtggtgat cggtcgcagt gccgaagaga tggcgctggc ggtcaatcag gtgattcagg
     7681 atggcggcgg gctgtgcgtg gtacgtaacg gccaagtaca aagtcatctg ccgttaccca
     7741 ttgccgggct gatgagcacc gacacggcgc agtcgctggc ggaacaaatt gacgccttga
     7801 aagccgccgc ccgtgaatgc ggtccgttac ccgatgagcc gtttattcag atggcgtttc
     7861 tttctctgcc agtgatcccc gcgctaaaac taaccagtca ggggctattt gatggcgaga
     7921 agtttgcctt cactacgctg gaagtcacgg aataataaaa aaagcccggc gtcatgccgg
     7981 gcaaaagtca ccagttacgt ttatgccact gtcaactgct gaattttttt ctcgcggcgg
     8041 attttgcgtt cttccattac cgccactatc gccatcaggc agatacaacc aatcgcggcg
     8101 atatccagcg cggcgaaggt gcctgcccag ccggtaaggc cgaataccgg cgtcccatcg
     8161 gcaatcattc ccagacctaa cttggcaaag ctgtcaccaa tcaggtaagc aaaggtgcct
     8221 ttaataccat cggcagcgcc aatcgctttt ttaggtacaa agccaacagc agccacacca
     8281 atcaacaatt gcgggccaaa gaccaggaaa cccaacgcaa agagagaagc cagatagata
     8341 tattcgttac tggcatgttg atacacaccg agcgtggcga taatcagcgc cagcgcgatg
     8401 caggccacca ggccacggcg accgttcgcc aggtcagaga gccagcccca cagcagcgta
     8461 ccgaccagcg caccagcttc aaacagcgta aagccctgaa tcgccaccgc tttagagagt
     8521 ttcagttcct ggaacgcgta tacggttgac cactggtcga taccaatacg taccacatag
     8581 aggaaaatgt tggcgaagca cagcagccag atcactttgt ttttcagcac atactcaaca
     8641 aagatctgcc acttggtcat atcggtagat tctgtctctt tgtcctcttc gctgacctcc
     8701 tcgccgaaca gttcttcagc tttgccgagg ccataagatt ccggggagtc gctaccgtaa
     8761 cgcaggccga taaaaccgac aatcagcgcg ataatcgacg ggaagataaa catgccgatg
     8821 acatggccat cgaacaggta atttgccccg aacagcgcca cacctgctgc gcctgcaccg
     8881 ccaaggttgt gagaaatatt ccagaaaccg aggaatgtcc cgcgtttacg acgcggcgtc
     8941 catttggtga tggtggagta actgcacgaa ccgccggtac tctggaaaaa gccgcttaag
     9001 gcgtagaagg caatcatcag gaacaggcta accgagccgc tgcccatact ggcactgaag
     9061 cccagcatac aaatggcaga gaggataagc atgaacggca ggaattgttt ggtgtttttg
     9121 ccgtcggcgt agtatgaaac caacgtttta cccacgccat aagtgatgga gaaacccagg
     9181 ccgatcatcc ccagctgcgt catgctcaac ccgtaggtcg aaatcatatc gttctgcgcg
     9241 atgttaaagt tcttgcgaat caggtacatc gtcaggtagc cgataaagac caccaggtag
     9301 gattgcatga acggtttgaa ccacattttg cgccgcactt cgagcggaag gtccagggtc
     9361 ggcttgcgaa cctggtttag gaaagccagc atgggttact cctgaaatga atacctgcgt
     9421 gaggcatgca ttgtaaaaat cagcagcaaa tcaggcctga gatagcgtcc aagaaaaacc
     9481 tggaaaattt cttagttttg ccggatatga agtgaaaagg tgagatgcat cacgcttcac
     9541 gcggtgtctg ggcgttcaaa aagggcagta acagcagtgc ggaaatcccg gcggcgatag
     9601 cgataaccac aaaaaatccg ctccagtgcc aggtatcgag cactttcgcc agcggccaac
     9661 cagcaagcga cgcccccaga taagcaaaca agccgacaaa ccccgtcgcc gcccctgccg
     9721 cctctttgtg ggaacactct gccgccgcca taccaattaa catctgcggg ccaaagacaa
     9781 aaaaaccaat ggtgaagaag caggttgcct gcatcacgta gctggcaaat ggcatcagcc
     9841 acagcgagcc gactgaaagc aaaattccgg cggcgaaaat caaattcatc ggcccacggt
     9901 tgccgttaaa caatttgtcc gagccccaac cggctaccag cgcaccgata aatccgccca
     9961 gttcaaacat cgtcactgcc gtattcgccg tgaccagatc gacgcccagc gtctcggaca
    10021 tatacaaatt gccccagtcg ttgatcgccg cccggaccac atagaccagc acatagcaaa
    10081 acgaaagcag ccagatatac ggattcagca acacatattt ggtgaggatc tctttacgcg
    10141 tcaaccctgc cccttcttgt tgttgagcaa tttccagcgc gtcgtgccgc cattcaccga
    10201 ccgccggtaa acctaacgcc tgcgggcgat cgcgtagccg ccagcagaga aaaatcccca
    10261 cgactatcgc catacaacca gcaatcatca tcccggcacg ccagccgtaa tgcagcgcgc
    10321 tcgctgccat cacaatggga atgagtgcgc cgccgacgtt atgcgccgtg ttccataatg
    10381 cccaccaacc gccgcgctcg gtacgtgaat accaggccgt taacagacgc gcacacaccg
    10441 gtgaacccca gccctggaaa aaggcgttca gcacccagag cacggcaaac gcccatagcg
    10501 acgtcgagaa gccaaacaga atgttcatga tgcccgtggc gataagccct atccccataa
    10561 aataacgggc atttgagcga tcgctgacaa tgccggagac aaacttcgac acgccgtaag
    10621 taatgtaaaa cagggtcgct aacaggccga tatcgctacg gctgagcacg ccgttagcaa
    10681 ggatttctgg taccgcggcg ttaaaacttt tccgcgtgaa gtaaaacagc gcgtaaccca
    10741 gccagatggt cagcagaata tgccgacgcc agtagcgata acgggcatca atttcatgtt
    10801 tatcagtcat taatggcgca tcggcaggcg ctttcagaaa cggcaacatc atcgaacctt
    10861 agacataacg ttgaggtaga gaaacgctga cacgcgtgcc gtgcagacag gaaatgtgta
    10921 atgtgccgcc cagcgccgtt acgcgctcgc gcattccggt gaggccaaaa ccttgttgcc
    10981 cggaacccgg cggcaaaccg ctgccatcgt cttcaatcac cagcatcaac cgttcatcct
    11041 gctgccagcc ttgcagcgta accgcgctgg catcggcatg tttcacaatg ttgttcagcc
    11101 cttcctggca gacacgaaac agcgtcacgc gctggttttc gcttaatgct gattcatcga
    11161 tacgccattc gagatggctg acaatgccgc gcccttccag ctccatttcc cgcatcagtg
    11221 agcggatggc ctgctccagg gtgagatcat ccagctggcg cggacgtaac cgtcctaaca
    11281 aacggcgcac cgcgtcgtaa acgcccagcg acagttgttc gatgagctgc ccgctctgct
    11341 tcacactggc gttatctgcc gccagccgct gaacaatgcc cgcctgagta cgaatagcag
    11401 tgatggtctg accgatatcg tcgtgcagct cacgcgccac gtcgcggcgc acgctctctt
    11461 cggtttctaa caaacgttca gccagatgct gattgcgcgc cagttccttt tgtagcgact
    11521 ggttaagttc acgcaaccgc tggatgcctg cgcccagtaa caaccctgtc agactttgca
    11581 ccagcaacga gagtaataaa tccaccagat gatcgcgcca ggtttgactg gcgatcagcg
    11641 cgatggcgtt catcaacgtc gcaatcagcg ctccttgcca gccatagtgc caggccagcg
    11701 cgataatcgg cagcgccaga cagaatggcg taaagcgcga cagttcgtcc ggcaatccca
    11761 actggagcca gagactgata acaaacagca gcaagtacca gaccagatgc cgcccgcgcc
    11821 agttgatggg ctgagaaacc agtgacgggc cgagcggcag ccaggtgtta ttggcgagat
    11881 agtgccagaa caccagacat atcggggcca gcgtcagacc gccagttaaa gtcagcaaca
    11941 gcgcattcca cgactctttg ccgtgccaaa gccagggcag cgactgcaac aacgccgccg
    12001 ccgttaacgc cgcaccctgt aacagcaagg tgcgccagtc acgctgatgg cgatagcgcg
    12061 agatcagcgc tacgggcagt aacgtcagta aactaccgat catcaataac ggaaaatggg
    12121 ttaaaccgac cgcctgcgtt agccagtaaa tcagcagcca ctccgcaccc agcaatacgg
    12181 gccagtatcc gcgcgggcat tgcagcatta gccccagacg cagaccaaac ggaaataaca
    12241 gcaccgccat atcagggcgc tcaaccagat gcaggctgat actccatagg caaaaccatg
    12301 cggcagagaa gataaaaaag caggcaataa cggtaattaa gcgggagaac aacgtcttca
    12361 tcaccagcca tcaaacatgc ggcgcgccag ctctacgtcg ttactgacgc ccagtttttc
    12421 catcagattg gcgcgatgga cgtgtaccgt tttcggtgac aagcccagtt cggcggcaat
    12481 ctctttcacc gccattcctt gcgccagttt ttccgccacc tggcgttcac gtttggtcag
    12541 cgggtcctga cgaccggatg ccagtttaat ggcaatatcc ggcgtcagat aacagccgcc
    12601 cgtggcaacc gtatgcaccg cagcaatgag ttcatccggg ctacagcgtt tggaaagaaa
    12661 gccgcgtgcc cccgcgttaa gcgcctgctc aaccagcgca ggactgtcgt gaacggagag
    12721 cataatcgtc gccatacctt tcggcagctg gcttagcagc tccagaccgg agatatcggg
    12781 catggagata tcgcaaatac acacctgcac accgcgcccc ggcagccccg ccagcgcctc
    12841 gcgccccgaa ccaaactcgg caactacctg caaatcaggt tccagcccca gcagctgcgc
    12901 aaagccggag cggacgatga ggtgatcgtc tataagggca acggtgatca tggtcttgtc
    12961 ctggcgggta aaaaaaaacg cgcttacctt aacgataagc gcgatgttgt tcaagccttg
    13021 agcggttact gaaaaaacac cgcgatcttg ttaaacatcg tcggatcgga ctgattacgc
    13081 tgcactttca cgacatcttc cagcttatcg atttggctta tcatctgctc cagacgctgg
    13141 tcgtcattga ccagtagcca gatatggctt ttgtcgctgt cctgaatcgg cagacaaaga
    13201 atgccttcaa cgttaaaagc gcggcgggca aaaaggccac aaacgtgggt cattacgccc
    13261 ggatggttgc gaacggtgag ctccagaatt acgttgttat gagttgtgtt ttgcatggct
    13321 tattccccca ccatttcagt attcgccgca cctggcggca ccatcgggta aactttttct
    13381 tcggcatcaa tgcgcacatg gatcagcgcc gggccagggc gattgattat ttcctgcaat
    13441 gcagcctgcg gatcggcttc gttattcaaa tcacaggttt cgaggccgaa tccggcggca
    13501 atctgcataa agttgatttt gcccggatag gtggcggcaa aaacgccttg ctcgtagaac
    13561 agactctgtt gctgatgcac cagccccagc gcttcgttgt tcatcagaat gattttgaca
    13621 tccagctgat tttcactggc ggtcgccatc tcctgaatat tcatcatcag gctgccgtcg
    13681 ccggagaaac acaacacttt gcgatccggg ttcgccagcg ccgcgccaat cgccgcaggc
    13741 aggccaaaac ccatcgtgcc cagcccaccg gaggtcagcc actggcgtgg gcgattgagc
    13801 ggataagctt gcgcggtcca catctgatgc tgacccacat cggtggtgat aatcgcattg
    13861 tcatcgacac aggcggcaac ggcgttgatc aggccgtaat ggcttaatgg atcgcacgct
    13921 ttcgggattg gacacgggaa ttcgcgctgc aaatccgcta ccaactggtg ccactctgca
    13981 cgcagttgcg cttccacctg cgggatcaat tgcgccagca cgtcatcaac atccgcctga
    14041 atcgccacat gcggctgctt gattttaccc agctctgcgc gatcgatatc gacatgaata
    14101 attttggcat tcggacagaa ctgctcggtt ttgccaatcg cccggtcatc aaaacgcgca
    14161 ccgagcacaa tcaacaaatc cgcctcttgc aaaatatagt tggtactgcg cacgccgtgc
    14221 atccccagca tacccagcga caacggatgc gcttttggca gcatgcccag cgccattaaa
    14281 gtcatggtgg taggcagttg cgctttctcc gccagttcac gcacccgcgc aggcgcattg
    14341 atcacaccac cacccagata aagcaccggg cgtttggcag cgttaatcat tgtagctgcg
    14401 tcacgaatgc tttcttcgct aaaggagggt gcagcggctt tttctgccac cgcgggctga
    14461 gcttcaatct caaaaaccgc cgtttgtaca tccttaggaa tgtctatcca caccgggcta
    14521 gggcggcctg attgcgcaat gcggaaggca tcgctcatga cctgtgggag ctcttcgata
    14581 tgtctgacca gatagttgtg tttggtgatg gggatagaga tgccgtaggt gtccacttcc
    14641 tggaaggcgt cggtgccgat catcgaggcg ggaacctgac cagtgatgca aatcagcggg
    14701 atggagtcca gccgcgcatc agcaatggcg gtcaccaggt tagtcgcacc cggcccgcta
    14761 caggccatac agaccgccgg tttaccgtcg gtgcgcgcca ttccctgagc gataaacccc
    14821 gcgccctgtt catggcgagc cagaatatgg cggatttgcg tgctttggct taaggcgtcg
    14881 taaacaggca ggatagaacc gcccggaatg cccgtcacaa tcttaatgcc ctgctgttcc
    14941 aggaaatgaa cgataaattc tgcgccggta aagcgcttac gcgtcgatgt tgtgcccgaa
    15001 cttgccatgc tccagtcctt ttcttctggg ccgactttcc gggaggtgct taaacgaaaa
    15061 accccgcccg gtttgcgccg gcggggtttt ggaatcgtgt gttgttccag tccctacggc
    15121 gcattgccga cgaccaccac cacacgcacg acgaccactg cggcggatgg cgcagttggt
    15181 agtagttttg cgttgaacat ggaagtagtc attggggacc ttgttggttt tgtgtttaac
    15241 aatatttata caagcacagc tttacagggg agacaatgga aaatttttca gcaagggaaa
    15301 attgaggggt tgatcacgtt ttgtactgaa ttgcagataa caaaaaaccc cgccggagcg
    15361 aggtttcgtc agccgcctgc ggctggtaac cgcaaagcac actgtattat gtcaacactg
    15421 aaagtatacg tgttccgcgc agaacgcgca atttcggcac gaattttgac gtatttagtg
    15481 catagttgag tatcgttcac agtttgcgtt ttgtccaaat attactgttt atttatacag
    15541 taaacttcta taatatcact gttcgcaatg tgttatgcgg gggccgcatc gttacccggc
    15601 gcactaagtc ctggctgaaa cgggtggtgc cgtcagcgcc ttaaccccgc gtgagcacac
    15661 tgtgttatgt caacaagcac aacgtttctc cttgagatac cgcgtgcaca acagctggca
    15721 acaggcagcg gaaaggtacg tcagctggca gtgctcctga accacaggag acgcgtatga
    15781 gcctggtgga tatcgccatt cttatcctca aactcattgt tgcagcactg caactgcttg
    15841 atgctgttct gaaatacctg aagtaattca gattcaagtc gcaccaaagg ggagcgggta
    15901 accgctcccc ttttatattt agcgtgcggg ttggagtcgg atgcgatgct gacgcatctt
    15961 atccgcccta ccatccctcc cggcaacatt tattgccgct tttgtttaca tattctgccg
    16021 ctaaacaatt ccccattcct ggcgtatatc tggctaacat tcatcaatgt gatagattcc
    16081 tctcccgcat ttatgggaat gcgtagtgac ttattctaat tatttttata aaagcatccg
    16141 tgataatgaa aaggcaaaga aacgtcaatt tgttattgat gttggtatta ctcgtggccg
    16201 tcggtcagat ggcgcaaacc atttatattc cagctattgc cgatatggcg cgcgatctca
    16261 acgtccgtga aggggcggtg cagagcgtaa tgggcgctta tctgctgact tacggtgtct
    16321 cacagctgtt ttatggcccg atatccgacc gtgtcggtcg ccgaccagtg atcctcgtcg
    16381 gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac gacctccagt ttgacggtat
    16441 tgattgccgc cagcgcgatg caggggatgg gcaccggcgt tggcggcgta atggcgcgta
    16501 ctttgccgcg tgatttatat aaacggacac agttgcgcca tgctaacagc ctgttaaaca
    16561 tggggattct cgtcagtccg ttgctcgcac cgctaatcgg cggtctgctg gatacgatgt
    16621 ggaactggcg cgcctgttat ctctttttgt tggttctttg tgctggtgtg accttcagta
    16681 tggcccgctg gatgccggaa acgcgtccgg tcgatgcacc gcgcacgcgc ctgcttacca
    16741 gttataaaac gcttttcggt aacagcggtt ttaactgtta tttgctgatg ctgattggcg
    16801 gtctggccgg gattgctgcc tttgaagcct gctccggcgt gctgatgggc gcggtgttag
    16861 ggctgagcag tatgacggtc agtattttgt ttattctgcc gattccggcg gcattttttg
    16921 gggcatggtt tgccgggcgt cctaataaac gcttctcaac gttgatgtgg cagtcggtta
    16981 tctgctgcct gctggctggc ttgctgatgt ggatccccga ctggtttggc gtgatgaatg
    17041 tctggacgct gctcgttccc gccgcgctgt tctttttcgg tgccgggatg ctgtttccgc
    17101 tggcgaccag cggcgcgatg gagccgttcc ccttcctggc gggcacggct ggtgcgctgg
    17161 tcggcggtct gcaaaacatt ggttccggcg tgctggcgtc gctctctgcg atgttgccgc
    17221 aaaccggtca gggcagcctg gggttgttga tgaccttaat gggattgttg atcgtgctgt
    17281 gctggctgcc gctggcgacg cggatgtcgc atcaggggca gcccgtttaa gcgcacgtca
    17341 ccgcagcatc gtcatcagct ccatgggaga acgatgctgc tttatcagat cacgcatcac
    17401 ccgcatatgc ggtgcggagt aagaataaaa cgcctgatag cccgcacaaa gcacgctgtc
    17461 tggcgtctcc tgccgagcgt gcgggcacag gcgatgccag tcgcacgcct ggcagtcacg
    17521 gcgcggttgc gggttttcgc tgccacacca tttttgtaag gtggcgtcaa aaagctcaat
    17581 cgagatacga ctgatatctg cccgcaccca acactcaaac agggcaatta aaaatttccc
    17641 ccacagctct gctgtcaccg aagcggccgt cagatcgccc cggtcatcaa attcaagatg
    17701 gggaatgagc tgtacggagc ggatgccccg ctgtttgaaa ttgtgataaa aatcggcagg
    17761 ttgcagagca tccttgtact ctacaaccag cttatgcctg tcctcttcag catcaatgac
    17821 ctgacttcct gtcatgcgac tctctcaata aatacgataa gttgatgtat atgcgttaca
    17881 gcaaacagta ttgcgagaga taacaccgcg agggagatca aaaatttgtc acggactacg
    17941 tgaaattggg aaaaatcgct attcgtgaca tccattaaat tgcgattacg ggtgtagtgc
    18001 cagaggatca gcgccacgat ggcgaggatg ccaatcgaaa tccagaataa catgcccgcc
    18061 tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc cgtagcccag catggtacga
    18121 aaccacgcca gtgatgtgcg ctccggctgc agccctgggt cggcaatgcg tctggcttta
    18181 cggctatccg gcatacaaca ccagtcccat aacgatcacc gccacaacca tcaatattaa
    18241 gctgatgatt aataagctgt tggtgtaggg caggtcctct ttcagacgca tcgctttttc
    18301 attgcgtaac cagcgcaggt agccatacat tgctaacccg ccggaaaaca gacacaacaa
    18361 caacgccagc agttcacgaa tgacgggcgt ggcgaaatct ggcgcaagtt gatcaagccc
    18421 aacccccgcc gccagaaatc ctagtgcggt acggatccac gtcagaaaag tacgctcatt
    18481 agccagcgag aagcggtaat cgggcgcttc tccaaggcgg gaaattttca ttgttgctcc
    18541 tttgcaattt cactaacgct ctatctttag cggttttttt taagaatttc gtccgcccgt
    18601 ttcctgatat gcttttgcca tccgctacaa ggaggtcaga tgggtatcat agcgcaaaat
    18661 aaaatcagtt cattgggcat gctgttcggt gccatcgctt taatgatggg gattatccac
    18721 ttttcattcg gccctttttc cgcaccaccg ccaacgcttg aaagcatcgt ggcggacaaa
    18781 acggctgaaa ttaaaagagg tcttctcgcc ggaataaaag gcgaaaaaat taccactgtg
    18841 gagaaaaaag aggacatgga tatcgataaa atcctcgatc aaagcggtat cgcactggcc
    18901 attgctgcgc tactttgcgc atttattggc ggtatgcgta aggaaaatcg ctggggcatt
    18961 cgcggcgcgc tggtttttgg tatcggcatc gtgtgcagca ttttgctgat attcctgatt
    19021 ttttccttcc tgaccggcgg ttcgctggtt taagcagcct gaataatgcc cgccgggtga
    19081 tcatcagcgg gcattatcgt cagaatttct gttttttctc ctccaccttc actccttgcg
    19141 tcggtaggcc ggtgtcatag tcgcgtacca ctggcgaata gccatcttgt gggcgtgggc
    19201 gaaacgcccc catccagcgc ggtcgtgcat ctttacgcca cggacgcaga ctccattggt
    19261 aactgcggaa cggatcgcga attttgtcca tgtaatccaa taaggcgtca tgcattttgc
    19321 tgcgaacgtc tgcaaaacgg atatcatcga tcaggttatg catttcattt gggtcattac
    19381 ggcgatcgta aagttcatca ctggtgaaga ggttgagcac cagtttaaag tcatccgtca
    19441 cccagcaacg caccggaata aaaccgccaa agctgtcatg ctcaatctcg tagcggttaa
    19501 attccaccat cacgccgcgt ggctctttca cggcaaggat attttccccc ggcagaatct
    19561 ctggtttttc aatatctgcc agcgccatca ttgtcggcag taaatcaata tgactgactg
    19621 gcgtatcgac ctgtcgccgc tcctttactg atcagcttat gtgcgcccat catttcaccg
    19681 tgatcggagg tataaatgac ccacgtattt tcacgttgct ctggcgttaa ggcattgatg
    19741 acacgtccga tttggtcatc aacaaagtca ttgcaggcaa aatagagcgg atggtgataa
    19801 agcccgtcat caccgactgg cgatggcatc gcctgcgccc ataagcggtg atgttccggt
    19861 ttgttcgcca ggtcatcctg tgatttctcg ccaagttcgt agtaaaaatc agcgtatttc
    19921 tctaaatact ccaccggaca ggtgaacggg tgattcggct catcatacga aaccaccatc
    19981 aggaagggtt cctcggcgcg cacgggctgt tgcagaaaat ctaccgctcg attgctgatg
    20041 cggtgcgccc aggtgaaggt ttcgtcaata tggttcgcct gtaaatcttc gacgctgttt
    20101 aggccattgc gccacaggct gatctctttt tccgtcagtt cgctaagata gttcgcccca
    20161 tcaaaccagt aatcagcatc ccactccggc ggacactcgc cagtgccgaa atagtcatga
    20221 ccgtcgagat gccatttgcc gatgtaacag gtgtgatagc cggcatcctt aaagtagcgc
    20281 cccatagtgg agatgttttt gcctggcgcg acgttgttgg tccacgggcc ggactggtta
    20341 gcgtagatac cggtgaatag tccggcgcgt gcaggcgtac aaaccggtga acaagtgtag
    20401 gcggaattaa agcgaatacc ttccgccgcc agactatcaa tattttgcgt attcagcggt
    20461 ttaccgctat agcaaccgac catattggtg gcctgggtat cggtcaattg gggcgtttca
    20521 taggctatcc ttcgcgtcgg gagtaaaggt tacggctggg tcgtgacgat gccgccagct
    20581 gtcaaaaatc aggtagacaa tcactacggc agcgatgaaa taactgatca tcgctaacca
    20641 gcgcgtaccg tagccgccga attcagccag cccgacatag acgccaatca tcgcgaacag
    20701 gataccaatt gacgcgatct tgacgttttt ccacggtttc atgtccaccg caaacgcatc
    20761 tttgaaggtg aacggcgtgg cgcgcggttt gataaaaccg atcaccagca tcacgaccac
    20821 gttgatgcag aacgtacagg ccagcacata gaggaaatgg aagtcgaact tcaccagata
    20881 gttgatggtg atgtagctga ttatgccaat ccccatcgcc acttttgccg ccagcgccgg
    20941 gatgcgcggg aagaaaaagc ccataatgat gatggtaacc agcggcacgt tgtaaatgcc
    21001 gttgagctgt ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc acggcgctac
    21061 caaaaccgaa acgatagcga taaagaaacc aaatttgcgc ccgacggtga ccagctgctg
    21121 cggctcggca ttctggttaa tgatgcgacg gtaaataccc atactgaata atgtactggc
    21181 gctattcaga aagccgttga aggtactgat caccgcacca aataacaccg cgccgaagaa
    21241 acccaccagt ggcactggca gaacgttatt gaccagcgtc gggtaggcca tgtcggcttt
    21301 cggtagatcc tgatacagat gaaacgcaat caaccctggc agcaccagta ccagcgggtc
    21361 gagcattttc agcaccgccg ttaacagcgc ccccttttgc ccttccgcca ggctttttga
    21421 cgccagcgtg cgttgcacga tgccctgatt tgtacaccag taaaaggtgt tcaccagaat
    21481 taaaccggta aatgccgcgc caatcggcaa gggatcggtc gggccaccga ctgagtttaa
    21541 tttctcggcg tgaacggtgg taatttgctc aatgccctgc ataaagctgc ccttgcccat
    21601 cgcgatcagg ccaaataccg gcaccatcaa cccgccgata accagcccaa taccgttgat
    21661 ggagtccgcc actgccattg cgcgcagtcc gccgatcacc gcatacaaaa ttcccgccag
    21721 accaagcaaa attaccagta gccagatagc cgcaccgtga gaaatctgta gcgattcccc
    21781 gacgtgaaac aggctgttga gcgccaacgc gccggagtag agaacaatcg gcagaaagca
    21841 gacgccggtg gcaatgagga agcagaagtc gatgataata cgcgtcgttt tatcataacg
    21901 ttcctccaga aaatcgggga tggtggcaat gccgcgcttc agatagcgcg gtaaaaagat
    21961 tagcgcgagg aagatcagcg tcaccgctga agtcacttcc cagcccatca ccgacatgcc
    22021 gcttttgtag gcctgcccgg aaagaccaac cagttgttcc gtggaaaggt tggttaacat
    22081 taacgaagcg gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat agccttgttg
    22141 cgatccggta tctgttttgc gaaccttcca ctaggtgatc accgccacca gcagcgtaaa
    22201 accgacaaaa ctcaagattt gtaacgaatt catctcatag cccttattta tgtttatgtc
    22261 ccggacgcat gaatataggg agaggtaaaa tgtgtcgggt caggcagtca gattcttaaa
    22321 atacaaacgt cgtatccctg aacggattgt tttctgttaa gttcaggttg tgagcataat
    22381 tcgccggagg gatattaaaa tgaatggaaa attgcaaagt tcagatgtaa aaaacgaaac
    22441 tccgtacaat attcccttat tgattaacga aaatgtgatc tccagcggaa tttctctgat
    22501 ctcgctctgg catacctacg ccgacgagca ttaccgggtg atctggccgc gggacaagaa
    22561 gaaaccgctt atcgccaact catgggttgc ggtgtatacc gtacaaggat gtgggaaaat
    22621 tcttttaaag aatggcgaac aaataactct gcatggcaac tgtattatat ttttaaagcc
    22681 aatggatatt cactcttatc actgtgaagg tttagtcttg gaacagtact ggatggaatt
    22741 tacccccacc agtatgatgg atattcccgt tggtcagcaa agcgttattt ataatggcga
    22801 aatttataat caggaactca ccgaagttgc tgagttaata acttcaccag aagcaataaa
    22861 aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt gtcttatgtc
    22921 tgcagatggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg ccactttaca
    22981 tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc cctgtagcga
    23041 agcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag aatattacct
    23101 cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caaggaaact cggttggcga
    23161 agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct ttaaacataa
    23221 atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa cggatgccag
    23281 tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca actcatccag
    23341 aatagctttg gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg cctgccacag
    23401 gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg ccacttgctg
    23461 actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt caggccgtag
    23521 tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat ccggctttca
    23581 ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag acgacgcgtg
    23641 gcttctgcga caattgctgc cgggttggag tagttgagca tctaggcgtt tggtgaatat
    23701 ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc gtacgctatt
    23761 ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gccgtgggat tttttcatcc
    23821 agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca taacaaaatc aacgtcgctg
    23881 aacgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt ttctttaagg
    23941 atgactttac aggcttcggc aatcacttcc tgccgcgcgc catcgttatc ataaaatttc
    24001 agtgcacgaa gcgggaaacg gtcctgattc gccaggagca tcaacacgat gcctggcgta
    24061 aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact gcctccgtaa
    24121 tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt tcacgcaatt
    24181 gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag acgccgtgcg
    24241 ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc agtgcaatac
    24301 gtagacgcgt cgcgcaattg ttaatgctgg agatattgcc gaccccgccc agggcttgca
    24361 ggataccggc agcctgatcc agctcttttt ttggctctgc agcggtggtt tagcctcgcg
    24421 aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt cccggcgtgc
    24481 acatgttgaa ctgcagaatc agggggcgga aaaccacgaa gtacagcagg gtaaagcaca
    24541 acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc gggatccagt
    24601 tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca ccaaagagat
    24661 acattacggt cgacattgag gccgccagca cggcgtgtac cgcaaacagc aacggtgaaa
    24721 tgaacaggaa ggtaaattcc agcggttcgg taattcccac cagcatggcg gttaaggttg
    24781 cgggaatcag caagcccgcc acttttaccc gattttccgg tgcggcagtg aagtacatcg
    24841 ctaaagaaat gcccacggca ccaaagattt ttgagttacc gtgcagggca aaaccgcctt
    24901 ccgggaacaa cgatttcagc ggctcggcgc tcagactgaa ctcttgcaga tgctgcgccc
    24961 agtacatctg aatgccgcct tcaacagctg ccggaccaaa gataaacggt ccgtagatga
    25021 agtggtgtaa accggttggg atcagaatac gttcgaggaa ggtgtaaacc cacaccccaa
    25081 gcgcacccgc cgaacgcagg aacgcttgca gagattcaat ccccatttgt acttttggcc
    25141 agccgagcaa tgtcagccag gcacagggga tcatcaccag gaaggcgata atcaccacat
    25201 aagacgtccc ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac agacggttat
    25261 gcagcgccgt cacaatgccg gaaatgataa ttgcgccgat aatgctggta tcgagggttt
    25321 taatcccggc catcattgtc agaccgctac ctgccaccgc gtcctgagtg aaatcgacgc
    25381 cgaagtagct tccccaggtc attcccatcg cattgatgaa atagttccag gtcaggaaac
    25441 tcaccatcac cgccagacaa gcacgtccct gcgcttgctt agcaaggcca atgggtaaac
    25501 cgacagcaaa aatcagcggc atattacgga ataccgtcca accgccctct tcaataatgt
    25561 gtacgatttg cgcgaataaa ctgttcggat cggtcagtga ttccccgaca aacatcgggt
    25621 tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc agcactggcg
    25681 tgaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc tcttttctca
    25741 attctgtaag taggaagcct gattggttat gcggcagacc tggagcgagg ataggaagat
    25801 gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc ttttcgttgg
    25861 gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg tacacagcat
    25921 cgaacacatc tttaaaaaaa agattttttt caatcgatta agcagaactt gtgggcgcat
    25981 tacccgtgct tgcaggcaaa aaagagagct agagatgatc tacaaaagca ttgcggagcg
    26041 gttaagaatt cgacttaatt ccgcggattt cacgctaaac ggccttcttc ccggtgaaaa
    26101 aaagctggcg gaagagtttg cggtatcgcg gatgactatc cgtaaagcca ttgacctgct
    26161 ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc acttacctgg tgcgcaaaga
    26221 tgtgctgcat caaaccgcca gcctgaccgg actggtggag gtgttaaaac ggcagggaaa
    26281 aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg ccattgccag
    26341 ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc gttttgtgga
    26401 agggaaaccg ctaatgctgg aagacagcta tatgccggta aaactgttcc gtaacctttc
    26461 actgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg ggattttgat
    26521 tggcggtaat tatgaaagcc tgatgccggt gctcgccgat agactgctgg cgcggcaaat
    26581 gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata gcgagagcgg
    26641 ggagtttttg aattattcag tgatgttcag aaatgccagc gaatactagg tggagtacca
    26701 tttacggcga ctccacccgg aaaagagtta accgatactc cagaagagca ccgccagtaa
    26761 ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt aagagagcgc
    26821 cgccgcacca ctggttggat gaagattatt agcaaacgcc agcgccggag gatcggtcat
    26881 ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca tccgcgccag
    26941 aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa tccagcttag
    27001 cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac caacgaccga
    27061 gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg gcataaacca
    27121 gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca gcggaccgcc
    27181 cgccagcccc agtttcaacg cggccgggaa tcctggcaca aagacgggaa tagaacctaa
    27241 caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt gcagtttttg
    27301 ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc ccaccaggtt
    27361 gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga ccccggcacg
    27421 gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt
    27481 tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt
    27541 agacagcgag gtatcgacct cctgaccaat caccagttgc gcattatgta aatccgctgg
    27601 ctgccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg aaggtacttt
    27661 tagggtttct tctcgtttca gacgcgagca gataattttg tcgccgttga gaatcggtac
    27721 atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga tagtcttgat
    27781 cagcgcgccg ccattggtgc gtgaagactc gtgctgctga gcttctgtct cgacattgac
    27841 gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc cgaatggata
    27901 cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac ccaggtcgcg
    27961 caagatctgc tgccctgccc ccagcgctgg cgtattggtt accgcaccgg agaaaatccc
    28021 cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa ccagaccacc
    28081 gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc
    28141 aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca ggccaaattc
    28201 ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc
    28261 gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc gaaatttgac
    28321 gttgccgata aacaaaccga cgactgccac caaagccaga atactgaccg ttaatgctat
    28381 atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt atggctgaat
    28441 cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc
    28501 ttaaacagtc gaaatttaac gcattgttga agaagaaaga caaaaaaagc cccaccagaa
    28561 tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc tgatagcgat
    28621 acgctgcgct gcgatgggtt ctggctcatt acgaattaaa tcaatatgca gtaaaccgtt
    28681 tacgaaggtt gcgccagaga cttccatatt ttcagccagc gtaaaactca ggctaaatgg
    28741 ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct ccggcgtgcc
    28801 ttttacgctc aggcgcgtac cttccagttg aatctctaaa tcttcctgac ggaaacctgc
    28861 cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt acggcgggaa
    28921 gctctggctt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac cgatccattg
    28981 acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta agaagcgagt
    29041 aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg gagatgtcag
    29101 gccgcgccag gcggccttag ggaattagtt gatttcgata cggcgcggtt ttttcgcttc
    29161 cggaatcacg cgttcgagat cgatatacag caaaccattt accaggttag ccccacgaac
    29221 atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga tgccctggta
    29281 cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca gcagattatc
    29341 ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag caatgcggta
    29401 atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac tctggctctg
    29461 gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt aaagcgggga
    29521 taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta agaccttcca
    29581 caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcga ctacgatgta
    29641 aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg actttttttt gcagtttatg
    29701 gtctattgca tagactgagg gggcagcacg aaacgttaaa gtgcgatagc cgccacagtg
    29761 cgacgtaatg atgatgttat tttcagcaag ggatcgctat aactcatcag gccaacaccc
    29821 acgatcaaca gatgaattta ttatgataag aaatgtgttg ttagcgttca tgatatgcag
    29881 cggaatgaca ttactcggag gatgctccag cgtgatgtcc cataccgggg ggaaagaagg
    29941 aacgtatccc ggcacccgcg ccagcgccac catgattggc gacgatgaaa ccaactgggg
    30001 ctctaagtcg ctggcgattc tcgatatgcc gtttaccgct gtgatggaca cgcttttact
    30061 gccgtgggat gtgttccgca aagacagctc ggtgcgctcg cgcgttgaaa aaagcgaagc
    30121 caacgcgcaa gccaccaatg cggttattcc accggccaga atgcctgata actgatttag
    30181 cgatcggttt cggtgatcca cagctggcca ccttccatcc acgccagcca ttgaccatcc
    30241 ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt ggttttcggt taaatactca
    30301 acctcgccgc tttgcgcatg ggcacaaaca attcgattat ccagcacaaa gcccaaccat
    30361 tctcctgacg gatgccagtt aaatgcagac tgaatatccg ttttgttatg ggttaacttg
    30421 cgcggttcgc cgccctgtgg cgagataagc cacagttgca caatgccgtt atcatcacgc
    30481 attaaaaacg cgatttgcgt accctgcgga ttacagcgca cccagtggcg cgggacgttg
    30541 actaaccccg gataagcccg atgatgggta aaggttaaac gtcgctgcac gacgccacgc
    30601 ggtggcgcgg gtagggttgt ttccgttcca cttaacggcg catcacctgc cgatttccag
    30661 ctagcttcat cttgcggtaa ctcaacgata aatagctccg gcactttctc gccctttggc
    30721 gaaagtgtgt cgccaataaa cgccagcgcg tgatttccta cccatccttc ttcataagca
    30781 cgattgattt catcactgcc aggctgcggc gtgggcgtgg ttttactcac cagcacacac
    30841 cagtggctac cgctgtattc acgcggatgt tgcttttgta cgttgaccgg gccaaacggt
    30901 gcagcaaccc cgacgtttcg caaatccagc gccggatcga gttgatgcat tacatggtcg
    30961 ttataggtaa agctccccct ttcaccgttc gggctaaaga catgcacatg gctgccaccg
    31021 cgcagcgctc ctggcgtata cggcgcggta atatccattg catcgagatt gctcaccttg
    31081 ccgccttcag caatgactcc gcgtcgatga tggaagtcgt aatgccatgt ttcatcagga
    31141 ttttcagggc cgtgaataaa gacatatttc tctgacttcg gatgaacggt caccacgccg
    31201 acgtgtgcgc cctgtgacgc gcgatagata acctcgacct cgcctgtatg gatattcaca
    31261 cgctcaatgg tctcgccggt aaacgacgcc ccagaaggac gcacgtcaaa taccagccac
    31321 tggctgtcgg gcgtccaggt attggtattg gtgagcaggt gattacgggg agcaaaggtt
    31381 atctgtttca tggcgcggtc ctgatacaaa acggttcccg ccatcatact tcacaaggac
    31441 ttcactttca gacaatagtc ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat
    31501 ttcgacgtgg cgattattgg cctcggcccg gcagggtcgg cgttggcacg aaagttagcc
    31561 ggcaaaatgc aggtgatcgc gctggataaa aagcaccagt gcggtactga aggtttcagc
    31621 aaaccttgca gattttcagc gtcaaaactg tcgacgtcgc cgcatcgctc acgcgtaact
    31681 accagcgaag ctatatcaat attaatcgcc atgctttcga cttgtggatg aaatcgctga
    31741 tccccgccag cgttgaggtt tatcacgata gcctgtgccg aaaaatctgg cgtgaggatg
    31801 ataaatggca tgtcattttt cgtgcagacg gctgggagca acatattacc gcccgctatc
    31861 tggtcggtgc cgatggcgca aactcgatgg tgcggcgaca tctctacccg gatcatcaaa
    31921 tccgtaaata tgtcgctatc cagcagtggt tcgcagagaa acatccggtg ccgttctact
    31981 cctgcatctt tgataatgcg ataactgact gttactcatg gagtatcagc aaagatggtt
    32041 attttatctt tggcggtgcc tatccaatga aagacggtca gacgcgtttc acgacgctga
    32101 aagagaaaat gagcgccttt cagttccagt ttggcaaagc ggtgaaaagt gagaaatgca
    32161 cggtgttgtt tccctcacgc tggcaggatt ttgtctgtgg taaggacaac gcctttctga
    32221 ttggtgaagc ggcgggattt atcagcgcca gctcgctgga ggggattagc tatgcgctgg
    32281 atagcgcaga gattctgcgt gcggtgttcc tgaaacagcc ggagaagagc aacgccgctt
    32341 actggcgcgc cacacgcaaa ctgcgtttaa aactcttcgg caagatagta aaaagccgat
    32401 gcctgaccgc accggcttta agaaagtgga ttatgcgcag tggtgtggcg catattccac
    32461 agttgaaaga ttatccaacg cgcttcacat cacccaccag caggatgtaa gagagcgcgc
    32521 caatcaacgc gacggcggag atataaacca gtgcaggcgc gaaaccgtaa ccctgcgcca
    32581 ggtaccccac caccagcgga acggtgatgc cgcccagacc accggcgaag ttaaacacgc
    32641 cgccggttaa accaatcagg cgcatcggtg ccagagaaga gaccagcgac caggtaatcg
    32701 aagcaaaacc gttgccgaag aatgccagcg ccatcaggca cataatcatc atcggatcgt
    32761 tggtgtagtt agcgcccata atgcaggtgg agatcagcaa gccgcagata atcggcgttt
    32821 tacgcgcaaa gcccagtgaa aagcccttac gtaccagcag atccgcgacc cagccagaga
    32881 gcaggacgcc gacaaacgcc gcgaggaatg gcaccgtggt cataaagcct gctttcagcg
    32941 ccgtgattcc tttttcctgg gttaaatagt tcgggaacca ggttaagaaa aaccacagtg
    33001 cagaagccac cgcaaattgc ccaagataaa cgccgatcag tttacgatgg aacaccagtt
    33061 tccagtcttt ggctgttaac ggctgacgcg cctctttctt caccggcgca tcaccatcca
    33121 ccagaccgcc gccatcacga atgtaatcca gttcagcttt gctgatacct ttggtcaggc
    33181 gcggcggctg ataaacctta aaccaaatca gcgaccagat aatgccgata ccaccagtga
    33241 caatgaacac ccagtgccag ctcaacatct cctgaatcca gatcagcagc ggagtcaaaa
    33301 acgccagacc gacaaactga ccagacgtat aaaaaccaac ggcggaagcg cgttcatgtt
    33361 ccggaaacca gctggtcacc atccggttat tggtcgggaa agcgggcgct tcgaaaatac
    33421 cggttatcgc acgcagacca attaatgaca ttaatcccgt ggcaaagccc tggaataaag
    33481 tcgccactga ccaaccaaat atcgcaataa aataagtcac gcgagaacct acgcgatcta
    33541 aaaaccaacc gccggggatc tggcatagcg tataaagcca ggcgaaggcc gaaaatacat
    33601 agcccatttc cgctttcgta atgccgaact cttcctgaat atgggcggaa gccacggcca
    33661 ggttagcgcg gtcaacataa caaatgacca ccgtaataaa gatcatcacc agcgtcagat
    33721 aacgccgacg ccccggcttt gctgcattaa cgggaatatc catagcgagc tttctccaga
    33781 ttttgggcat agcgaagccg ctcaccatgc cctgtaattt acagagggtt atatttttgt
    33841 attgctgttt tagtgcccga tgaggggctt acgtggcagg aattaccact ctgctacgct
    33901 gttatcttca tgacgccaga gcggattacg ccagtccggg gcatttttac tgaactcaat
    33961 aactttagct tcgtcgattt ccacacctaa gcccggtttc gttaacggtt taaagaagcc
    34021 gccaaccatg ctgaagtctt ctttgttttt cacaaagtcg agtaactccg cgcctttgtt
    34081 gtaatgaatg cccatacttt gttactgaag taccgcgtta taggaaacaa agtcgatatg
    34141 caggcaagcc gccagtgcaa tcggtccgaa cggacagtgc ggcgcaaggg tcacatcata
    34201 ggcttctgcc attccagcaa ttttgtagca ttcggtaata ccgcctgcat gggagagatc
    34261 cggttgcaga atcgaaatac cgcctgcctc cagcacgcgt ttaaaatcga aacgtgagaa
    34321 catgcgctca cccgccgcca gtggaatatg cgtttgtgcc gccaatttcg ggtagtattc
    34381 ggcttgttcc gccagcaccg gctcttcaat aaacagcggg cgatacggct ccagttcttt
    34441 aatcagcact ttcgccattg gcgcgctgac gcgaccgtgg aaatcaagac caaactcaat
    34501 ctgattgcca aaagcttcac gaatttgtgc cacggtgttg actgccgcat ctaccgcgcg
    34561 ggagttatca attagcccca gttcttcaca accgttcagt ttgaaggtat cgaagccgat
    34621 ttcgcgcagg gttttaatgc cgtcgataac atccgccgga cgatcgccgc cgacccaact
    34681 gtaggcttta attttgtcgc gaaccaggcc gcccatcagt tgccagactg gcgcattcag
    34741 cactttgcct ttgatatccc ataacgcctg gtcgatcccg gcgatggcgc tcatcaggat
    34801 tggaccgcca cgataaaatc cggcgcgata catcacttgc cataagtcgt tgatgcgcga
    34861 aggatcctga ccaatcaaat agtcacccag ctcgtgaact gccgcttcca ccgtacgggc
    34921 gcggccttca atcacgggct cgccccaacc gaccacgcct tcatcggttt caattttcag
    34981 gaacatccag cggggaggta aacgatacgt ggtaattttg gtgattttca ttgcactgcc
    35041 tctcgatacg ccttaacaaa tgctgctgcc tgctgcgcgg tgcgttctac ggactgcccg
    35101 gcgcgataaa gatcgctgcc taagcccgcc ccaacacaac ctgcgtctat ccactgcgcc
    35161 aggttttctg gcgtcacgcc gccaacggca aagactgcga tgtccggtgg taataccact
    35221 tttaacgctt tgatgtattg cggaccaaaa gccgatgacg gaaatatttt cagcgcctgc
    35281 gcgcccgctt cgagcgcggt aaaggcttcg gtcgccgtcg cgcagccggg gcaaacggtc
    35341 atgccgtagc ccaccgcgcg gcggatcact tcactatgga tattgggcgt aacgatgagc
    35401 tgacagccca tcctggcgag cgcatcgacc tgttcaggtt tcagtaccgt acctgcgcca
    35461 atcaacgcct tgtcgccata cgcatcaacg atggcgggaa tgctctgctc ccattgtggg
    35521 gaattcagcg ggatttcaac cgcgtcgaac ccggcgtcaa tcaccgcgcc aacatgcgcc
    35581 agcgcctcgt cgggcgtaat gccgcgcaaa atggcgatca gcgggagttt agtttgccac
    35641 tgcatgagcg atgctcctta taccagcctg aaatgccgtg tcgcccgcca ccgccgccac
    35701 gtcgcaaccc atcgcctgaa aggcttgctg gtagcgcgcg gtcagcgatg ttccggcgac
    35761 aagggtgatg gcgtgttgat gggtcacata gtcgcgcata ctggcaacct ctgcgccaat
    35821 caacaaacca gagagaaatt cgctgacctg ttcgcggaga agtgttccca gcacatgcga
    35881 ggcgcgaact tcaaaaagct gcggcaatat ggcgggcgca ttaaggccgc gctcaaggcc
    35941 agccgcgaag gcatcggcag agttttcctg cggcggcaaa cctgcgccaa tcaatgagtg
    36001 atttaacagt aaatgatgta attcaccggt catcacggtg cgaaaatcgt tgatttgctg
    36061 gctatcggcc tgcacccatt tgcaatgcgt accgggcata acataaagag aggaaggagc
    36121 cagagctcgc gcgccgatca attgtgtttc ttcgccgcgc atcacattgt ggttatcgtc
    36181 atgagagacg cataatccgg gaataatcca gatattgtcg ccaacagacg ttaattgttc
    36241 gccaatagac gaaaaacggg caggaacaga taaatacgga gcaactttcc agccgacatt
    36301 gctgccgacc attcctgcca tcaccactgg cgtattttct tcacgccagt cggtcgtgac
    36361 ttctgctaac accgcagccg gagattttcc gttcaggcgc gtgacgcctg cttctgattg
    36421 cctgctctcc aggcagtggt cgccctgata aagccaggcg cgcagattgg tcgatcccca
    36481 gtcaattgcg atgtagcgag ctgtcatgtg atttccttta accttcgtgt cgagctggca
    36541 atcatggtaa gcgccgcctg ctctgccgca tcgccgtcct gatgccgtat cgcatcgaac
    36601 agcgccttat gttcctggag cgtttgcggc atgttggcct catcgcccat ccaggttcgt
    36661 tcaaaaaccg cccgctgcag cgaactgatc gcaatgctaa gttgctgtaa caccgggtta
    36721 tgcaccgact gcagcaccgc ctcgtggtag cgaatatccg cttcgttaaa cgcttcgcgg
    36781 tcctgattgt tggcaatcat ctcgttcagc gccgattcaa tctgcgccag atcgctggag
    36841 gtcgcgcgct ctgctgccca acgggcaatc gccggttcca ccagatttcg cacttcgctc
    36901 atggcactga taagccgtgg gtcgtagtcg ttttccagca cccattgcag tacgtcagtg
    36961 tcgaggtaat tccactggtt acgcggtgcc acaaacgccc cgcgataacg tttcatttca
    37021 atcagccgct tcgccatcag cgaacggaac acctcacgga tgatgttgcg cgaggttgca
    37081 aactcttcac agagctccgc ctcagccgga agtggcgagc caggtacgta tttgccgtga
    37141 acgatctgtt tacccagcgt aatgacaatg cgatcggttt tattgagagt catggagagt
    37201 ccttgtgctc tgtatgttct tctctacttt accccgatcg atgcataacg cggcaacttt
    37261 gtagtaccag cgtgatgacg ttcgcgtttg ccgtgcgtgt aatgtagtac aaacttatat
    37321 tgttgtgcta caatttagat caaaaaaaga acaatgtata aaaaaatgac atgcgtcggg
    37381 cagaaatctg aaaagggata tcaggcgcta aacaggaggg aaagaagagt atgctttcaa
    37441 cggcttagct actcgtttaa aagattaatc atgaagttga attttaaggg attttttaag
    37501 gctgccggtt tattcccact ggcgctgatg ctttcaggct gtatctcgta tgctctggtt
    37561 tcccataccg caaagggtag ttcaggaaag tatcaatcgc agtcagacac catcactggg
    37621 ctatcgcagg caaaagatag taatggaaca aaaggctatg tttttgtagg ggaatcgctg
    37681 gattacctta tcactgatgg tgccgatgac atcgttaaga tgctcaatga tccagcactt
    37741 aaccggcaca atattcaggt tgccgatgac gcaagatttg ttttaaatgc ggggaaaaag
    37801 aaatttaccg gcacaatatc gctttactac tactggaata acgaagaaga aaaggcactg
    37861 gcaacgcatt atggttttgc ctgtggtgtt caacactgta ccaggtcact ggaaaaccta
    37921 aaaggcacaa tccatgagaa aaataaaaac atggattact caaaggtgat ggcgttctac
    37981 catccattta aagtgcgatt ttatgaatac tattcaccca gaggcattcc ggatggtgtt
    38041 tccgcagcat tactgccagt gactgttacg ctggacatca ttactgcacc gctgcaattt
    38101 ctggttgtat atgcagtaaa ccaataatca gtaagcgggc aaacgcgttt atgctgtttg
    38161 cccgcccaca gattaattca gcacatactt ctcaatagca aacgccacgc catcttcaag
    38221 gttggatttt gtgacaaagt tcgccatttc tttcaccgaa ggaatagcgt tatccatcgc
    38281 cacaccgacg cctgcatatt caatcattgc gatgtcgttt tcctgatcgc caatcgccat
    38341 gatttcttcc ggtttaatac ctaacacgtc ggccagtgat ttcacacccg tacctttgtt
    38401 aacgcgttta tcgaggattt cgaggaagta cggcgcactt ttcagcacgg tatatttctc
    38461 tttcacttcc tgcggaatac gcgcgatagc ctggtcgagg atggcgggtt catcaatcat
    38521 catcactttc aggaactggg tattggggtc cattttctcc gcttcgcaga acaccagcgg
    38581 aattgtggca acgaaggatt catgcaccgt gtagtagctg atatcgcggt tagcggtgta
    38641 cagcgtggtg cggtccaggg cgtggaaatg agaaccgact tcgcgagaga gtttttccag
    38701 gaaacgatag tcgtcatagc tgagagcggt ttgcgccacg gtgctaccat cagcggcctt
    38761 ctgtaccagc gcgccgttat aagtaatgca gtagtcgccc ggctgttcca tatgcagctc
    38821 tttcaggtag ttgtgcacac ctgcatacgg acgacccgtc gttagcacga cattcacgcc
    38881 acgggcgcga gctgcggcaa tcgcattttt aacggcgggt gaaatggtgt gatcgggcag
    38941 cagaagggtg ccatccatat cgatagcaat gagtttgata gccatgagtt cccaaggtag
    39001 attagttcct gacccatgct aacgcgattc cgctcaaaaa tcagtacaac acccgaggga
    39061 aaaggggatg caacgcgggt tgcatgctcc ccatttgctt agcggaagag tttccctttc
    39121 agcagttcca tgcctgcgga aagcagatcg ttattggctt gtgctgacac ttcaccttgc
    39181 ggtgagagcg catcaataat cttcggcaat tgctctgcca gtaaactgga agccgtactg
    39241 gtatccacgc caagtttttg cccgagatcg gacaccgcat ttgtgccgag cgccgattcc
    39301 agttgctcgc cgctaactga ttgatttctc tgttgattac tcaaccaagt tgagagaatg
    39361 gcccctaagc cgccactttg cagtttttcc agcagcacct gaatgccacc ctgctcctca
    39421 acccaactta aaatcgcctg atatttcccc gcatcgcctt tcagaaaggc accgacaact
    39481 tcatcaaaaa gccccatgat aatcacctgt aaagcgttac gtgttgaccc aaaaagtata
    39541 gatctgcggg atgataattg cggattgcag aaataaaaag ggcggagatg atctccgccc
    39601 ttttcttata gcttcttgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg
    39661 caaaatcaat atattgcagg agtctcgtag gactaataag cgtagcgcat caggcacgct
    39721 cgcatggtta acgccattaa atatcaatat tcgccgcttt cagggcgttc tcttcaataa
    39781 acgcacgacg cggttctacg gcgtcgccca tcagcgtggt gaacaactgg tcagcggcaa
    39841 tcgcatcctt aacggtaacg cgcagcatac gacggctttc cgggtccatg gtggtttccc
    39901 acagctgttc cgggttcatc tcgcccagac ctttataacg ctggatggag aggccacgac
    39961 gggactcttt caccagccag tccagcgcct gctcgaagct ggctaccggc tgacgacgct
    40021 cgccacgttc gataaacgca tcttcttcca gcaagccacg cagtttctca cccagcgtgc
    40081 agatacgacg atattcgcca ccggtgataa actcgtgatc cagcggatag tcagtatcca
    40141 caccgtgggt acgcacgcga acaatcggct cgaacaggtt ttgttcggca ttggtgtgga
    40201 catcaaactt ccactggctg ccgtgctgtt ctttgtcgtt cagttcgctg accagcgcgt
    40261 tcacccagcg ggtaacggtc tgctcatcag aaaggtcagc ttccgtcaac gtcggctgat
    40321 agataagctc tttcagcatt gctttcggat aacgacgctc catgcggttg atcattttct
    40381 gcgtcgcgtt gtactcagat accagtttct ctaacgcttc gccagccaat gccggtgcac
    40441 tggcgttggt gtgcagcgtt gcgccgtcca gcgcgataga gatctggtac tgatccatcg
    40501 cttcgtcgtc tttaatgtac tgttcctgct tgcctttctt cactttgtac agcggcggct
    40561 gagcgatgta gacgtgaccg cgttcaacga tttccggcat ctgacgatag aagaaggtca
    40621 acagcagcgt acgaatgtgc gagccgtcga cgtccgcatc ggtcatgatg atgatgctgt
    40681 gataacgcag tttgtccggg ttgtactcgt cacgaccgat accacagcca agcgcggtga
    40741 taagcgtcgc cacttcctga gaagagagca tcttatcgaa gcgcgctttc tcgacgttaa
    40801 ggattttacc cttcagcggc agaatcgcct ggttcttgcg gttacgcccc tgcttcgcag
    40861 agccgcccgc ggagtcccct tccaccaggt acagttcgga aagcgccgga tcgcgttcct
    40921 ggcagtctgc cagtttgccc ggcaggccag ctaagtcgag cgcaccttta cggcgggtca
    40981 tttcacgcgc gcgacgcgcc gcttcacggg cacgggcagc atcgataatt ttgccaacca
    41041 cgattttcgc atcagttggg ttttccagca ggtattccgc cagcagttcg ttcatctgct
    41101 gttcaaccgc cgatttcacc tcagaagaaa ccagtttgtc tttggtctgg gaggagaatt
    41161 tcgggtccgg cacttttacg gaaacgaccg caatcaggcc ttcacgcgca tcgtcaccgg
    41221 tggcgctgac tttggctttt ttgctgtagc cttctttgtc catgtaggca ttcagggtac
    41281 gggtcatcgc cgcacggaag cctgccaggt gagtaccgcc gtcacgctgc ggaatgttgt
    41341 tggtaaagca gtagatgttt tcctggaagc catcgttcca ctgcagcgcc acttcaacgc
    41401 caataccgtc tttttcggtg gagaagtaga agatatttgg gtggatcggc gttttgttct
    41461 tgttcagata ttcaacgaac gctttgatgc cgccttcata gtggaagtgg tcttctttgc
    41521 cgtcacgctt gtcgcgcaga cgaatggaaa cgccggagtt gaggaacgac aactcacgca
    41581 gacgtttcgc cagaatttca tattcgaact cggtcacatt ggtgaaggtt tcgaggctgg
    41641 gccagaaacg caccatggtg ccggtttttt cagtctcgcc ggtaaccgcc agcggagcct
    41701 gcggtacacc gtgttcgtag atctgacggt gaattttacc ctcgcgctga ataaccagct
    41761 ccagtttttg cgacagggcg tttactaccg aaacaccaac gccgtgcaga ccgccggaca
    41821 ctttatagga gttatcgtca aatttaccgc ctgcgtgcag aacggtcatg atcacttccg
    41881 ccgccgatac gccctcttcc gggtgaatac cggtcggaat gccgcgcccg tcatcctgta
    41941 cagagacaga gttatcggcg tgaatggtga cgataatttc tttacagtga cccgcgagcg
    42001 cttcgtcgat agcgttatct accacctcga ataccatgtg gtgcagaccg gtgccgtcat
    42061 ccgtgtcgcc gatatacata cccgggcgct tacgcaccgc atccagccct ttcaggactt
    42121 tgatactgga ggagtcataa gaattcgaca tcaacgtttc tcgctcattt atacttgggt
    42181 taatccgtta ttttaccctt ttccacggta aacatcttcg aattttcgtc cgacatgtct
    42241 ataacgtgtt cagcactgat cgcgctgaca aagacctgtg attgcgtcgc ttttaagcgg
    42301 ctggcaagca acccgcgacg ctcatcatca agctcagagg caaaatcatc tatcaggtag
    42361 agacaccgcc gcccgctttc acgagtgagg aactctcctt gcgccagacg taaggcgcac
    42421 atcaacagct taagctgccc acgcgataag gtatcttcca ccggcgcacc gtcggcgcga
    42481 atgcgtaagt ccgctttatg cgggccatgc gcggtgtagg ttagctggcg atcgcgttca
    42541 aaattacgtt ccagcacctc agcgtactct gtctctttct cccagccgcg ctggaaagag
    42601 aaagtcagag aaaactcagg gagaaattgc ttacaggtat catccatatc ggccgcgata
    42661 ccggcgctat actccgcgcg ccaggtgctg atttgctccg ccagcgggat cagttcttta
    42721 tcccacgggc gtagctgttc gtaacgagtc acctggcgca gcgccgcatt gcgctgcttg
    42781 agcaatcgct tgagattgct ccaggcggtg aaaaatctag gttcgttgtg aaagcatccc
    42841 cagtcgagga atgctcttct gtatttgggg ccgccgttga gtaaagtaaa cccttctggc
    42901 gttatcaact gcatcggcat caggtgcgcc agttccgcga ctttatgccc gtccgtaccg
    42961 tcaatacgga ctttgctgtc gccctgtttg tctttggtta agccaatcgc cgtctcgcgc
    43021 tcttcgccct gtaatcgccc gtggagaaca aatgcctcct gctcatggcg aatgacgcga
    43081 ccaatctgca aactgcgaaa cgcccgacca tggccgagcg tatagatggc ttccagcacg
    43141 ctggttttgc cactgccgtt ggcacctacc agaaagttaa agccgggaga taaagcgaga
    43201 tccgcggttt caatattgcg gaaatcgcgg atcaacaagc gggtgaggga cattacagtc
    43261 tcattggcat gacaacatag gcagccgact gtgatgcggc atcttcaatc tgcacgctgg
    43321 aaaccgaatc ggtcagcatc atgcggacgt tttcgcattt cagcgcgttc agaacatcca
    43381 gcacatagct gacgttgaag ccgatttcca tctccgcacc gctataggta acgtcgagga
    43441 tctcttccgc ttcttcctgt tccgggttgt tggcagtaat tttcagctgg ttttcgctga
    43501 catacaggcg cacaccgcgg aatttctcgt tagagagaat cgccgcgcga gcaaacgcct
    43561 gcttgagcag atcgcagcct gcttccagat gtttgtccgg gttcttcggc agaacgcgac
    43621 ggtaatccgg gaagcgacca tccaccagtt tcgaggtgaa aataaagtcg ccaacgtggg
    43681 cacgaatgtt attgctgcca atctgcacac gcagcagatt gtcgccgccg tcgagcatac
    43741 gcatcagttc aatcacgcct ttacgcggta cgatcaccga atggcttggc aaagattgac
    43801 caattggcat tgaacagacc gccagacggt ggccgtcggt tgccacggtg cgcagttctt
    43861 caccttcggt ttcaaacagc ataccgttta agtaatagcg aacatcctga tgcgccatcg
    43921 aaaactgggt tgcttcaatc agacgcttca tcgtcgcctg cggcagggta aattcgactt
    43981 cgctctgcca gtcatcgagg ttcgggaaat ccgccgctgg cagggtagac agcgaaaaac
    44041 ggctacgccc ggagcgcacc agcatccgtt caccttccag ctgcacggca atttccgcgc
    44101 cttcaggcag accacggcag atatcaaaga atttgcgcgc cggaacggtc gtcgctcctg
    44161 gctcgtgtgg ctgaaccagc gcaacgcgtg ccaccatttc catctcgaga tcagtaccgg
    44221 tcagcgacaa cgtaccgtca gcaacctgta acagcagatt accgagaatc ggtagcgtag
    44281 gacgaccacc taacggaccg ctcacctgtt gtagcggttt taataaatgc tcacgttcta
    44341 cggtaaattt cataggttta cgatgacaat gttctgatta aatttgaaaa atcttctttg
    44401 atatcgtggc tctcttcacg caactgctcg atcttacggc aggcatgaag caccgtcgtg
    44461 tggtcacgcc caccaaacgc atcgccaatc tctggcagac tgtggttagt cagctctttc
    44521 gctagcgcca tcgccatctg gcgcggacgc gccaccgagc gggatcgacg cttggaaagg
    44581 agatccgcga ctttgatctt gtagtactcc gccaccgtct tctgaatatt gtcgatggtg
    44641 accagttttt cctgcaatgc cagcaagtcg cgcagcgcct cacgcacgaa gtcgatggtg
    44701 atcgcccgtc cggtaaagtt ggcgttagca ataacgcggt tcagcgcccc ttccagctca
    44761 cgtacgttag atcgtagacg cttggcgata aagaacgcca cttcacccgg caaacgaatg
    44821 tcattttcgt cggccttttt catcaggatc gccacacggg tttccagctc tggcggttcg
    44881 atcgccacag tcagtcccca accgaagcgg gatttcaaac gatcctcaac gccgttgatc
    44941 tctttcggat agcgatccga ggtgagaatg atctgttgat taccttccag cagggcgttg
    45001 aaggtgtgga aaaactcttc ctgagatcgt tctttattag caaaaaactg aatatcgtcg
    45061 atcagcagtg catctacgga acggtagtag cgtttaaact cttcgatcgc gttgttttgc
    45121 agggctttaa ccatgtcctg aacaaagcgc tcggagtgca tataaaccac tttggcattc
    45181 ggcttgcgcg ccataatgcc gttacccacc gcatgcagca ggtgagtttt acccagaccc
    45241 gtgccgccat aaaggaacaa cgggttatag gcaccgccag ggttatccgc cacctggcga
    45301 gccgccgcgc gcgccagttg gttagattta ccttcaacga agttatcaaa cgtgtgtttg
    45361 acgtttacgt tagaacgata ggtcggttct gccggggccg ggacgttatc ccaacctgag
    45421 cgcgtagaag gcgcagcacg ttgcggctgc gtttgcgcca cctgtgcagg ggccgcgacg
    45481 ttgctcgtca ctgccgcttg tggcgtttgc gtcaccggtt tggtgccgac ttcaaaacgc
    45541 agctgtgggg catccgctcc gcagaaactg gttagcagtc cattgatatt attaaggtac
    45601 ttgtcccgta cccaatcgag gacaaaacgg tttggcgcgt acagggacag cgtgttatcg
    45661 ctcagttccg cctgcaatgg gcgtatccac atactgaatt ctgtggctgg taactcatcc
    45721 tgcaatcggg caagacactg ctgccaaagc gaaagtgaca cggcggactc cactcgaaca
    45781 aaagtcgata atgactaagg ctgaaacatt catgattgtt gacgtacgtc gaaaagaccc
    45841 tgcttgaggg tgacgcacga accgctgtct gcggttatat gcccgatcaa gatcctgcaa
    45901 aacgatcggg accgcggatc atagcctaaa ctgcgcaaga gatcttctgt ttctcacaga
    45961 ttttccctat ttatccacag gactttccag aactcgctaa gtgtaaacga tcctgccgcg
    46021 aggcgggcac gatttacgcc gcatattgga aaatttaatg accatagaca aaaattggct
    46081 taatcgatct aataaagatc caggacgatc cttgcgcttt acccatcagc ccgtataatc
    46141 ctccacccgg cgcgccatgc tggtttccac tggtgtgagg ccgtacattt tccctgcgaa
    46201 aaggtgcgga aaagcgcggt aaataaggaa agagaattga ctccggtgtg tacaattatt
    46261 acaatccggc ctctttaatc acccatggct tcggtgtcca tcgtttcatt tttcggcgga
    46321 tatccaataa agccattgaa tttattcaag tttaggtaga aatcgccatg aaacgcactt
    46381 ttcaaccgtc tgtactgaag cgcaaccgtt ctcacggctt ccgtgctcgt atggctacta
    46441 aaaatggtcg tcaggttctg gcacgtcgtc gtgctaaagg ccgcgctcgt ctgaccgttt
    46501 ctaagtaata aagctaaccc ctgagtggtt aagctcgcat ttcccaggga gttacgcttg
    46561 ttaactccca gtcaattcac attcgtcttc cagcagccac aacgggctgg cacgccgcaa
    46621 attaccattc tcggccgcct gaattcgctg gggcatcccc gtatcggtct tacagtcgcc
    46681 aagaaaaacg ttcgacgcgc ccatgaacgc aatcggatta aacgtctgac gcgtgaaagc
    46741 ttccgtctgc gccaacatga actcccggct atggatttcg tggtggtggc gaaaaaaggg
    46801 gttgccgacc tcgataaccg tgctctctcg gaagcgttgg aaaaattatg gcgccgccac
    46861 tgtctcctgg ctcgcgggtc ctgatagccc tcattcgggt ctatcaacgc ctgattagtc
    46921 cgctactcgg gccgcattgt cgtttcactc caacctgttc aagctacgga attgaggcat
    46981 tgcgcaggtt tggagtgata aaaggcagtt ggttgacggt gaaacgcgta ttaaaatgcc
    47041 accctttaca ccctggtggt gacgatcccg tcccgcccgg accatttgat accagagaac
    47101 actaacgatg gattcgcaac gcaatctttt agtcatcgct ttgctgttcg tgtctttcat
    47161 gatctggcaa gcctgggagc aggataaaaa cccgcaacct caggcccaac agaccacgca
    47221 gacaacgacc accgcagcgg gtagcgccgc cgaccagggc gtaccggcca gtggccaggg
    47281 gaaactgatc tcggttaaga ccgacgtgct tgatctgacc atcaacaccc gtggtggtga
    47341 tgttgagcaa gctctgctgc ctgcttaccc gaaagagctg aactctaccc agccgttcca
    47401 gctgttggaa acttcaccgc agtttattta tcaggcacag agcggtctga ccggtcgtga
    47461 tggcccggat aacccggcta acggcccgcg tccgctgtat aacgttgaaa aagacgctta
    47521 tgtgctggct gaaggtcaaa acgaactgca ggtgccgatg acgtataccg acgcggcagg
    47581 caacacgttt accaaaacgt ttgtcctgaa acgtggtgat tacgctgtca acgtcaacta
    47641 caacgtgcag aacgctggcg agaaaccgct ggaaatctcc tcgtttggtc agttgaagca
    47701 atccatcact ctgccaccgc atctcgatac cggaagcagc aacttcgcac tgcacacctt
    47761 ccgtggcgcg gcgtactcca cgcctgacga gaagtatgag aaatacaagt tcgataccat
    47821 tgccgataac gaaaacctga acatctcttc gaaaggtggt tgggtggcga tgctgcaaca
    47881 gtatttcgcg acggcgtgga tcccgcataa cgacggtacc aacaacttct ataccgctaa
    47941 tctgggtaac ggcatcgtcg ctatcggcta taaatctcag ccggtactgg ttcagcctgg
    48001 tcagactggc gcgatgaaca gcaccctgtg ggttggcccg gaaatccagg acaaaatggc
    48061 agctgttgct ccgcacctgg atctgaccgt tgattacggt tggttgtggt tcatctctca
    48121 gccgctgttc aaactgctga aatggatcca tagctttgtg ggtaactggg gcttctccat
    48181 tatcatcatc acctttatcg ttcgtggcat catgtacccg ctgaccaaag cgcagtacac
    48241 ctccatggcg aagatgcgta tgctgcagcc gaagattcag gcaatgcgtg agcgtctggg
    48301 cgatgacaaa cagcgtatca gtcaggaaat gatggcgctg tacaaagctg agaaggttaa
    48361 cccgctgggc ggctgcttcc cgctgctgat ccagatgcca atcttcctgg cgttgtacta
    48421 catgctgatg ggttccgttg aactgcgtca ggcaccgttt gcactgtgga tccacgacct
    48481 gtcggcacag gacccgtact acatcctgcc gatcctgatg ggcgtaacga tgttcttcat
    48541 tcagaagatg tcgccgacca ctgtgaccga cccgatgcag cagaagatca tgacctttat
    48601 gccggtcatc ttcaccgtgt tcttcctgtg gttcccgtca ggtctggtac tgtactatat
    48661 cgtcagcaac ctggtaacca ttattcagca gcagctgatt taccgtggtc tggaaaaacg
    48721 tggcctgcat agccgcgaga agaaaaaatc ctgattcggt gagttttcgc taaaataagg
    48781 gcggtcagtt gaccgccttt tttcttttcg tagggcggat aagcaccgcg catccgccac
    48841 acaaagcaac aggaacatca tgagcgataa tgacactatc gtagcccagg ccacacctcc
    48901 gggacgtggc ggcgttggca tcctgcgcat ctccggcttc aaagcccgtg aagttgccga
    48961 aaccgtgctg ggtaaactgc ctaagccgcg ctacgccgat tatcttccgt ttaaagacgc
    49021 cgacggcagc gtgctcgatc aggggattgc gctatggttc cctggcccga actcgttcac
    49081 cggcgaagat gtgctggaac tgcaaggtca tggcggtccg gtgatcctcg acctgctgtt
    49141 aaaacgcatt ctgaccattc ccggcctgcg gattgctcgc cctggtgagt tttccgaacg
    49201 cgcgtttctt aacgataaac ttgacttagc ccaggccgag gcgattgccg atcttatcga
    49261 cgccagttcg gaacaggcgg cccgttcggc acttaactcg ctgcaaggcg cattctccgc
    49321 acgggttaat catctggtag aagccctcac ccacttgcgc atttacgtcg aagcggcaat
    49381 tgatttccct gatgaagaga tcgatttcct ctccgacggc aaaattgaag cccagcttaa
    49441 tgacgtgatg gccgatctcg atgcagtgcg tgctgaagca cgtcagggta gtttgttgcg
    49501 cgaagggatg aaagtagtga ttgccggacg tcctaatgcc ggtaaatcga gcctgttaaa
    49561 cgcgctggcg ggccgtgaag cggcaatcgt aaccgatatc gccggaacca cgcgtgacgt
    49621 gctgcgtgag catatccaca ttgacggaat gccgctgcat atcatcgaca ctgccgggct
    49681 acgtgaggcc agtgacgaag tggaacgtat tggtatcgag cgcgcgtggc aggaaattga
    49741 acaggccgac cgcgtgctgt ttatggtcga tggcaccacg acagacgccg ttgatccggc
    49801 agagatctgg ccggaattta tcgcccgtct gcccgcgaaa ctgccgatca ccgtggtacg
    49861 caataaagcc gatatcaccg gcgaaacgct gggaatgagt gaagtgaacg gtcacgcgtt
    49921 aattcgtctc tcggcaagga ctggtgaagg cgtggacgtg ctgcgtaacc atctcaaaca
    49981 gagcatgggc tttgacacca atatggaagg cggcttcctg gcgcgccgtc gccacttaca
    50041 ggcgctggaa caggcagcgg agcatttgca gcaaggtaaa gcgcaactgt tgggagcctg
    50101 ggcaggtgaa ctgctggcgg aagagttgcg cctggcacag cagaacttaa gcgaaatcac
    50161 cggggaattt acttcagacg acctgctggg gcggattttc tccagcttct gtattggtaa
    50221 gtaaccgcgc ttacgaagcc gtattctgac tgtcagatgc ggcttcgctt cattgttagc
    50281 actcctgtta tgcctcaacc cttttttaaa cattaaa
//
LOCUS       DAJTYF010000016        49926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTYF010000016 DAJTYF010000000
VERSION     DAJTYF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.016"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..494)
                     /locus_tag="OO590_001039"
     CDS             complement(<1..494)
                     /locus_tag="OO590_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR6636497.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATDHV"
     gene            complement(620..925)
                     /locus_tag="OO590_001040"
     CDS             complement(620..925)
                     /locus_tag="OO590_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6636498.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(1033..1109)
                     /locus_tag="OO590_001041"
     tRNA            complement(1033..1109)
                     /locus_tag="OO590_001041"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1073..1075),aa:Val,seq:gac)
     gene            complement(1114..1190)
                     /locus_tag="OO590_001042"
     tRNA            complement(1114..1190)
                     /locus_tag="OO590_001042"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1154..1156),aa:Val,seq:gac)
     gene            1498..2754
                     /locus_tag="OO590_001043"
     CDS             1498..2754
                     /locus_tag="OO590_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6636499.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(2795..4168)
                     /gene="mdtK"
                     /locus_tag="OO590_001044"
     CDS             complement(2795..4168)
                     /gene="mdtK"
                     /locus_tag="OO590_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6636500.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            4383..5024
                     /gene="ribE"
                     /locus_tag="OO590_001045"
     CDS             4383..5024
                     /gene="ribE"
                     /locus_tag="OO590_001045"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6636501.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(5064..6212)
                     /gene="cfa"
                     /locus_tag="OO590_001046"
     CDS             complement(5064..6212)
                     /gene="cfa"
                     /locus_tag="OO590_001046"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6636502.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(6503..7714)
                     /locus_tag="OO590_001047"
     CDS             complement(6503..7714)
                     /locus_tag="OO590_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6636503.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            7827..8759
                     /locus_tag="OO590_001048"
     CDS             7827..8759
                     /locus_tag="OO590_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6636504.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(8756..9781)
                     /gene="purR"
                     /locus_tag="OO590_001049"
     CDS             complement(8756..9781)
                     /gene="purR"
                     /locus_tag="OO590_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6636505.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            10080..10169
                     /gene="ynhF"
                     /locus_tag="OO590_001050"
     CDS             10080..10169
                     /gene="ynhF"
                     /locus_tag="OO590_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6636506.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            10335..11504
                     /locus_tag="OO590_001051"
     CDS             10335..11504
                     /locus_tag="OO590_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6636507.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(11650..12231)
                     /gene="sodB"
                     /locus_tag="OO590_001052"
     CDS             complement(11650..12231)
                     /gene="sodB"
                     /locus_tag="OO590_001052"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6636508.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(12359..13186)
                     /gene="mepH"
                     /locus_tag="OO590_001053"
     CDS             complement(12359..13186)
                     /gene="mepH"
                     /locus_tag="OO590_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6636509.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            13521..13868
                     /gene="grxD"
                     /locus_tag="OO590_001054"
     CDS             13521..13868
                     /gene="grxD"
                     /locus_tag="OO590_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6636510.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(13907..14407)
                     /gene="tnpA"
                     /locus_tag="OO590_001055"
     CDS             complement(13907..14407)
                     /gene="tnpA"
                     /locus_tag="OO590_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6636511.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            14415..15623
                     /locus_tag="OO590_001056"
     CDS             14415..15623
                     /locus_tag="OO590_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6636512.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQAESEVSTPVHPSASMVGLDAGVAKLASLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            complement(15667..20284)
                     /locus_tag="OO590_001057"
                     /pseudo
     CDS             complement(15667..20284)
                     /locus_tag="OO590_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(20377..21024)
                     /gene="rnt"
                     /locus_tag="OO590_001058"
     CDS             complement(20377..21024)
                     /gene="rnt"
                     /locus_tag="OO590_001058"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6636513.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(21127..21534)
                     /gene="gloA"
                     /locus_tag="OO590_001059"
     CDS             complement(21127..21534)
                     /gene="gloA"
                     /locus_tag="OO590_001059"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6636514.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(21615..22712)
                     /gene="nemA"
                     /locus_tag="OO590_001060"
     CDS             complement(21615..22712)
                     /gene="nemA"
                     /locus_tag="OO590_001060"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6636515.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(22749..23348)
                     /gene="nemR"
                     /locus_tag="OO590_001061"
     CDS             complement(22749..23348)
                     /gene="nemR"
                     /locus_tag="OO590_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6636516.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            23451..23690
                     /locus_tag="OO590_001062"
     CDS             23451..23690
                     /locus_tag="OO590_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6636517.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            23739..24635
                     /locus_tag="OO590_001063"
     CDS             23739..24635
                     /locus_tag="OO590_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6636518.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            24716..25045
                     /locus_tag="OO590_001064"
                     /pseudo
     CDS             24716..25045
                     /locus_tag="OO590_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
     gene            complement(25016..25399)
                     /locus_tag="OO590_001065"
                     /pseudo
     CDS             complement(25016..25399)
                     /locus_tag="OO590_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(25392..26183)
                     /locus_tag="OO590_001066"
                     /pseudo
     CDS             complement(25392..26183)
                     /locus_tag="OO590_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(26186..26422)
                     /locus_tag="OO590_001067"
     CDS             complement(26186..26422)
                     /locus_tag="OO590_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6636519.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            26623..27057
                     /gene="slyA"
                     /locus_tag="OO590_001068"
     CDS             26623..27057
                     /gene="slyA"
                     /locus_tag="OO590_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6636520.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(27104..27571)
                     /gene="slyB"
                     /locus_tag="OO590_001069"
     CDS             complement(27104..27571)
                     /gene="slyB"
                     /locus_tag="OO590_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6636521.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            27845..28954
                     /gene="anmK"
                     /locus_tag="OO590_001070"
     CDS             27845..28954
                     /gene="anmK"
                     /locus_tag="OO590_001070"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6636522.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            29052..29381
                     /gene="mliC"
                     /locus_tag="OO590_001071"
     CDS             29052..29381
                     /gene="mliC"
                     /locus_tag="OO590_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6636523.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            29440..30096
                     /gene="pdxH"
                     /locus_tag="OO590_001072"
     CDS             29440..30096
                     /gene="pdxH"
                     /locus_tag="OO590_001072"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6636524.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            30225..31499
                     /gene="tyrS"
                     /locus_tag="OO590_001073"
     CDS             30225..31499
                     /gene="tyrS"
                     /locus_tag="OO590_001073"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6636525.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            31558..32427
                     /gene="pdxY"
                     /locus_tag="OO590_001074"
     CDS             31558..32427
                     /gene="pdxY"
                     /locus_tag="OO590_001074"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6636526.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQV
                     KAANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAV
                     NNVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGM
                     RQPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDR
                     IAKPEHYFSATKL"
     gene            complement(32471..33076)
                     /gene="gstA"
                     /locus_tag="OO590_001075"
     CDS             complement(32471..33076)
                     /gene="gstA"
                     /locus_tag="OO590_001075"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6636527.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(33182..34684)
                     /gene="dtpA"
                     /locus_tag="OO590_001076"
     CDS             complement(33182..34684)
                     /gene="dtpA"
                     /locus_tag="OO590_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6636528.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(35295..35930)
                     /gene="nth"
                     /locus_tag="OO590_001077"
     CDS             complement(35295..35930)
                     /gene="nth"
                     /locus_tag="OO590_001077"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6636529.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(35930..36625)
                     /gene="rsxE"
                     /locus_tag="OO590_001078"
     CDS             complement(35930..36625)
                     /gene="rsxE"
                     /locus_tag="OO590_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6636530.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(36629..37249)
                     /gene="rsxG"
                     /locus_tag="OO590_001079"
     CDS             complement(36629..37249)
                     /gene="rsxG"
                     /locus_tag="OO590_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6636531.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(37253..38311)
                     /gene="rsxD"
                     /locus_tag="OO590_001080"
     CDS             complement(37253..38311)
                     /gene="rsxD"
                     /locus_tag="OO590_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6636532.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(38312..40438)
                     /gene="rsxC"
                     /locus_tag="OO590_001081"
     CDS             complement(38312..40438)
                     /gene="rsxC"
                     /locus_tag="OO590_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6636533.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(40431..41009)
                     /gene="rsxB"
                     /locus_tag="OO590_001082"
     CDS             complement(40431..41009)
                     /gene="rsxB"
                     /locus_tag="OO590_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6636534.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(41009..41590)
                     /gene="rsxA"
                     /locus_tag="OO590_001083"
     CDS             complement(41009..41590)
                     /gene="rsxA"
                     /locus_tag="OO590_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6636535.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFATIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(41667..42107)
                     /locus_tag="OO590_001084"
     CDS             complement(41667..42107)
                     /locus_tag="OO590_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6636536.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(42193..42408)
                     /gene="ydgT"
                     /locus_tag="OO590_001085"
     CDS             complement(42193..42408)
                     /gene="ydgT"
                     /locus_tag="OO590_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6636537.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(42681..42806)
                     /gene="blr"
                     /locus_tag="OO590_001086"
     CDS             complement(42681..42806)
                     /gene="blr"
                     /locus_tag="OO590_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6636538.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            43049..44089
                     /locus_tag="OO590_001087"
     CDS             43049..44089
                     /locus_tag="OO590_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6636539.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(44125..45126)
                     /gene="add"
                     /locus_tag="OO590_001088"
     CDS             complement(44125..45126)
                     /gene="add"
                     /locus_tag="OO590_001088"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6636540.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(45229..46401)
                     /gene="malY"
                     /locus_tag="OO590_001089"
     CDS             complement(45229..46401)
                     /gene="malY"
                     /locus_tag="OO590_001089"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6636541.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(46411..48003)
                     /gene="malX"
                     /locus_tag="OO590_001090"
     CDS             complement(46411..48003)
                     /gene="malX"
                     /locus_tag="OO590_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6636542.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            48178..49206
                     /gene="malI"
                     /locus_tag="OO590_001091"
     CDS             48178..49206
                     /gene="malI"
                     /locus_tag="OO590_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6636543.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            49318..>49926
                     /gene="hdhA"
                     /locus_tag="OO590_001092"
     CDS             49318..>49926
                     /gene="hdhA"
                     /locus_tag="OO590_001092"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR6636544.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFSWRCDITSEQELSALADFAVSKLGKVDILVNNVGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITP"
BASE COUNT        12617 a        12515 c        12941 g        11853 t
ORIGIN      
        1 acgtgatccg tggcaatcac cgctaaatca aacgtctcgc tgggtaaatc ctgatttgta
       61 gcgagcatga cgccagcatt tgtaatttgc agatcggtaa cctggcatga ttcataaaca
      121 gccactgcaa atttttgctg tcgtgcctgg tctactaatc gtaaaaattg atcgcggaaa
      181 tattcgccca gcagaattcg cggtaaaaac tgacgatcgt gcaaggtttc ttttttaacg
      241 ccataacgct ggagatggct ggcttcttgc ttttgtagcc attcgagata cgtacaatta
      301 atcggcggta tttcaatact ggcaatattt gccagcatca tttttgagtt ttcctcatca
      361 ctgtatggca tcccgacacc ggcctcgtca gcctgctcga agatagaaat agaaagtgga
      421 gtttgttgct gtagaagcga gaataaggtg tagatccccg taggcccggc acccacaata
      481 gcaatttttt tcattgttac ttacctgtac gcattctttc gaatttagtg ttatcagata
      541 agtttggttg agatttttag aatttgagaa atttcagaca gggatgaaaa gggcgctaac
      601 cgcccttgat taaattctgt caggcgagtt ttgcctgatt gatttgacta agtggctcat
      661 tgacatcgaa aactttggcg acaacttcct caacgccgag atttttcagt cgggcagtat
      721 gtttttccag ataggcaagg gcgctttttt catcagtgaa aaggtagatc ccaccggctt
      781 cgtggttctt ttcactttct gtccatacct tccacagaaa accaggttcc tgattaatcg
      841 actcagcaag tggtttaagc tgctcagcca ttgcgtcgcc aaatgggcca ttaaaagcaa
      901 aatgaaattg taacaaagtt gccatagcga ttctccttct gataacgcat acgaagacag
      961 tgtaaatctt ttaacgtatt ggatacagag aagaaacaga gggaaaaagg aaaagaccag
     1021 attaagaaaa tctggtgcgt ccgagtggac tcgaaccacc gacccccacc atgtcaaggt
     1081 ggtgctctaa ccaactgagc tacggacgca ggatggtgcg ttcaattgga ctcgaaccaa
     1141 cgacccccac catgtcaagg tggtgctcta accaactgag ctatgaacgc aacgttgtag
     1201 gtgacaacgg ggacgaatat tagcggcaga gtggaaaggt ggcaagaggc aaaacgtaat
     1261 tttctgcgct atttcgaccg tttgcagagc ttttaagcaa attggctata ttttgttgat
     1321 ttgcaagggc gatttttatt caggatcgca tttacatctg atacaaccca ataattcgtg
     1381 tatcagagtt taaacagagt tgtttgccac catctcaaat ctgtcgtgga taaatcctcc
     1441 gtagccttta cacttaggta gcaataaagg cacacagatg cagcaaagga ataaatcatg
     1501 ggatctgatg cgaaaaactt gatgagcgac gggaatgtgc aaattgttaa gaccggcgag
     1561 gtcattggcg cgacgcaact tactgaaggc gagttgattg ttgaagctgg cggaagagcc
     1621 gaaaataccg tggtcacggg ggctggctgg ttgaaagtgg caaccggtgg gatcgccaaa
     1681 tgcacacagt acggtaacaa tggcacgcta tcggtcagcg acggtgccat tgccacagat
     1741 attgttcagt ccgagggagg cgcaattagt ctctctacgc tcgctacggt taatggccgc
     1801 catcccgaag gtgaattcag cgttgatcag ggttatgcct acggtttgtt gctggaaaat
     1861 ggcggtaacc tgcgtgtact ggaaggacat cgcgcggaaa aaatcattct cgatcaagag
     1921 ggcggcctgt tggttaatgg gacaacctca gcggtcgtgg tagatgaagg tggtgaattg
     1981 ttggtgtatc caagtggaga agccagcaat tgtgagatta atcagggcgg cgtttttatg
     2041 ctggccggga aagccagtga tacgttgctt gctggtggca ccatgaataa tctcggtggt
     2101 gaagactctg acactattgt tgagaatgga tccatttatc gtctggggac ggatggcctt
     2161 cagctctaca gttccggtaa gacgcaaaac ctgtcggtga atgtggggga tcgggctgaa
     2221 gtgcatgccg gtacgctgga aaatgcggta atacaaggtg gaacagtgat cctgttgtca
     2281 cccaccagcg cggacgaaaa ttttgtcgta gaggaagatc gcgcaccggt tgaactgacc
     2341 gggagtgttg cattactgga cggcgcttca atgattattg gttatggcgc agagctgcaa
     2401 caatcaacga ttactgtaca gcagggcggt gtgttgattc tcgacggcag tacggtaaaa
     2461 ggtgacagcg tcactttcat tgttggtaac atcaatctga atggcggaaa actgtggctg
     2521 atcactggtg cggcaacgca tgtgcaactg aaagtgaaac gcctgcgcgg agagggagcg
     2581 atttgcctgc aaaccagtgc gaaagaaatt tcacctgact tcatcaatgt gaaaggggaa
     2641 gttaacgggg atatacgcgt tgagataaca gatgccagtc ggcaaactct gtgtaacgct
     2701 ctgaaattac agccagacga agacgggatt ggcgcaacgc tccagcctgc gtaaaaacac
     2761 aggcgcaacc cgtgaaggtt gcgccttgtc tttattagcg ggacgctcgt tgcagaatga
     2821 tggctgacgg catacgttgc aggaaccgca tacgcagcat catcataatg gctgccgatg
     2881 tcaggccgat aataaagcct atccagaagc ctgctggccc cataggttca acgaccagat
     2941 cggtcagtgc cagaatatag ccgcttggca agcccagcac ccagtaagct gtaaaggtga
     3001 tatagaaaat ggaacgcgta tctttataac cacgcaaaat cccactgcca atcacctgga
     3061 ttgagtcaga aatctgatat accgccgcca gcaacatcaa atgcgcagcc agcgttacaa
     3121 cctcgggatt gtcgttgtac aacagggcga tttgctcccg cagtgaaacc gtgaaaatgg
     3181 ccgtcagggt tgccatacag acacccacca taagcccggt ccgcgcagcg gtttgcgcat
     3241 ccagcgttga gccctgaccc agacgataac ctacgcggat agttaccgct gccgccagcg
     3301 acattggaag cacaaacatc agtgaactaa agttcagggc aatctggtgt cctgcgacat
     3361 caacaatacc gagcggagac actaacagag ccacgacggc aaacagtgtc acttcaaaga
     3421 acagtgccag cgcaatcggc aaaccgagtt gaatcagtcg tttcataacc gcaggatcgg
     3481 gcttagcggt gcctttttcg ttacgaatat cgcgcatgga gcgggcgcgt ttaatgtaag
     3541 aaaccatggc aaggaacatg acccaataca ccgccgcagt agccacgcca caaccaacgc
     3601 caccgagctc aggcataccg aaatgaccat aaataaagat atagttcacc gggatgttca
     3661 ccagcaggcc gataaagccc attaccatac ccggcttggt ttttgccaga ccttcacatt
     3721 ggttacgggc aacctggaag aacagatatc ccggcgcgcc ccacaacaac gcacgcagat
     3781 aacccacggc tttgtccgcc agagccggat cgatgttttc catggagcgg ataatgtaac
     3841 ctgcattcca cagcaccagc ataatgagaa cggaaacaaa acctgccagc cagaaacctt
     3901 gtcgcacctg atgcgcaatg cgctcacgtc gaccggaacc atttaattgc gcgataaccg
     3961 gcgttaatgc cagcagcagt ccgtgaccaa agaggatcgc cggaagccag atagaagtac
     4021 cgatagcgac cgccgccatg tcggtggcac tatagccgcc cgccatcacg gtatcgacaa
     4081 aacccatcgc agtttgggcg atttgcgcga gaatcaccgg gattgctaat gctaataaca
     4141 gacgcgcttc actgatatac ttctgcacgt gaacaccttt tatttgtagt tatatgagag
     4201 actaaaaagc cgcctgaatg ggcagcaaaa agaagaagca ggggaaattc agtctattgt
     4261 agcgaggatt tattaattct ccagtgaaaa aatagccatt cttacccctt cactggtaag
     4321 ggcgttttcc acctgctatt gtgctgaaca gaatgtctta actgatttca ggagttgtaa
     4381 gtatgtttac ggggattgta cagggcaccg caaaactggt gtcgattgac gagaaaccaa
     4441 attttcgtac gcatgtggtg gagttacccg accacatgct ggacggcctg gaaaccggtg
     4501 cttccgtggc gcataacggt tgctgcctga ccgtgacgga aattaacggc aaccatgtca
     4561 gttttgacct gatgaaagaa acgttacgca ttaccaatct tggcgattta aaagtggggg
     4621 attgggtaaa cgttgagcgt gcggcgaaat tcagtgatga aattggcgga cacttaatgt
     4681 caggtcatat tatgaccacc gctgaagtgg cgaaaatatt aacctcggaa aataatcgcc
     4741 agatctggtt taaagtccag gatagtcagt tgatgaaata tatcctgtac aaaggattta
     4801 ttggcatcga cggtattagc ctgaccgtcg gcgaagtcac gccaacgcgt ttttgcgtcc
     4861 atttaattcc ggaaacactg gaacgcacga ctcttgggaa gaaaaaactt ggcgcacgcg
     4921 tcaacattga aatcgatcca caaactcagg cagtggtaga tacggtagaa cgtgtgctgg
     4981 cggcacgaga aaatgccatg aatcaaccag gtacagaagc ctgatctgaa ctcgcccccg
     5041 aagagggggc gatagaatag cctttagcga gcgactcgaa ggccgttttc aacaccgcgt
     5101 gagaacacca cctgccagag ctgaatatca cgggcgcgga aagcacctgc gcaggcattc
     5161 agataatagg taaacatccg tttaaagcgt tcactgtagt tatccgcaat ttctggccat
     5221 gcggcgagga atcgttcata ccacgccatc aacgtagtat cgtaatcagc accgaagtta
     5281 tgccagtctt ccatcacaaa gtggggttcg ctggattgag caatctggcg tacagagggc
     5341 aggcaaccgt tcggaaaaat atatttatta atccagggat caacattcag atcggttttt
     5401 ttcgaaccga tagtatggag caggaatata ccttccggtt tcaaattacg atccaccacc
     5461 gcaaaatagg tatcgtaatt tttcggtccg acgtgctcga acatccccac agaaacaata
     5521 cgatcaaact ggtcgttcag gtcacgataa tcttgcagca aaatggtgac atccaggcct
     5581 ttacagcgtt cctgagccat tttttgctgt tcggcagaaa tggtgacgcc gaccacgctt
     5641 acgtcatagt tagatgccat gtagtgcgcc agtccgcccc agccgcagcc aatatccagt
     5701 acgcgcatcc ctggttttaa ctgcaatttt tcacaaatca ttttgagctt cgcctgctgg
     5761 gcagattcca gattatcggc atctttccag taagcgcagg aatattgcat gaagggatca
     5821 agcatgcggc tgaacaagtc attacccaaa tcgtaatgct ccttgccgac tatccaggca
     5881 cgttttttac tctgcagatt gaagagacga gcgccggcaa tacgcagcgt gtctttgaaa
     5941 tgatggggga gttggttctc gagacctgcg cgtaagactt tgctaaaaaa catatccagt
     6001 cggtcacatt cccaccagcc atccatataa ctttcgccta accccaaaga gccttcctgc
     6061 agaacgcgtt taaaaaaatc ggggtttttc acacgaatat ccgccggggc agaaccgtta
     6121 atggctatac cggcacggct aagtaattcg ttggcgatac ggtaccagtt gtcatccggt
     6181 acactgactt cttctataca cgatgaactc atagtttctc catcactggt cggtgatcag
     6241 aaccttaaaa cagcgtagac gctttttggg ctttgtgaga aatctcgcag aaaaaaccgc
     6301 gagtctataa tccgacgtag aacagaggga aaggaggaac ccttgccgaa atgaccatcc
     6361 atagtgagtc gggaacgatc ctgtccccgt aaaaacataa tatttatcgt agctttaaca
     6421 actcgaaatt agtataggcc gctaatttca atcatacaac aaaattttgt tagcagccta
     6481 agtataagta tatcgatata gatcagtgtg attcgctatg agcgacttcg gcattgccat
     6541 gattcgggca gtcaacttct tcacaacgtt gcatcatgta acccagcgcg accagcacta
     6601 ctgttgataa catcacgctg gtggtggtga gcaatggcgt gctgatacta ataagccagg
     6661 aaactaccag acttgcgagg aagcacagac ccagttgcag agtgttctgc aacgctgcgg
     6721 cgcgaccggt tgcatgtggg aagggacgca gcgcctgggc gacaacaata gggtagatcg
     6781 cgccattggc aatcgccatc acacagaatg ggatcaggat ttcgaccagc gacacatggc
     6841 taatgaagcc cgcagcccag gtcgcaatga cgctgacagc aaacagcacc agcaaccacg
     6901 gtaataactg cttgccttgc catttctgca gtgcggtgcg acagccataa ccaccaatca
     6961 gaaacgcgat agtttgcggg acataactta aaccaataac tgccgggctg tagcccattt
     7021 cactaaggat gaacggtgaa ccggtcagcc atgcaaaaaa actggctgaa caggctgcgt
     7081 atatcagcac gttgccgcga taggttttag aacgtagcag gtcggtaaag gtcagaccat
     7141 cctgactatt gttacgggcc ttcgtcgtgg gtttgagcca gaaaataggc agaatcagca
     7201 ccacggtaat ggcaaacagg gtggcgaaaa tcgcctgcca ggaaaaatgg accagcagcc
     7261 agcttcctaa caggggagcc agtgccggag acagacccac cagcggcatg atggtcgcaa
     7321 aaatacggtt gactttctgt gaaggataat aatctgtcac taatgcttgc cagataaccg
     7381 ccgcggcgca gacccccaca gcctgtacaa aacgcaatac cagcagcgtg gcggcgtttt
     7441 ctacccacag catccccaga ctacctaacg caaaaattgt caggccgatt aataacaccg
     7501 gtttacgacc ataacggtcg gagagtggcc cccacagaag ctgggctgcg gcaaaaccgg
     7561 caaggaacag actaaggctg gcactgacag cagacgcagg cgtttgcagg tcggcctgta
     7621 tggcggcgaa agcaggcaga tacatatcgg ttgccagaaa accgagtacg ctcaaacccg
     7681 ccagccagac taaaaatctt ttcccaggtt gcatgtatat tctctaaatc tctcaattaa
     7741 acaccgccgc aaagtgtagg gagtgaaatt agtgttgtga aacggtaata tttgatgact
     7801 ggtttcaaaa attttgcagg ctgactatgt ggtcagaata ttcactcgaa gttgttgatg
     7861 cggtagcgcg taatggtagt tttagcgctg cggcacagga gctgcatcgc gttccttctg
     7921 cggtcagcta taccgtgcgt cagctggaag agtggctggc ggtaccgctc tttgaacgac
     7981 ggcaccgtga tgtggagctg accgctgctg gagcgtggtt tctcaaagaa gggcgctctg
     8041 ttgtcaaaaa aatgcagatc acccgccagc aatgtcagca gatagcgaac ggctggcgcg
     8101 gtcagttagc tatcgcagtg gataatattg tcaggccaga acgtacacgg cagatgatcg
     8161 ttgattttta tcgccatttt gatgatgttg aacttcttgt ctttcaggaa gtgttcaacg
     8221 gtgtctggga tgcgctttcc gacgggcgcg tggaactggc aattggcgca acacgcgcga
     8281 ttccggtagg cggtcgttat gccttccggg atatggggat gctaagctgg agttgcgttg
     8341 ttgccagtca ccacccgctg gcgttgatgg atggcccgtt cagcgatgat acgttgcgca
     8401 actggccgtc gttggtgcgc gaagacacct cgcgaacgct acctaaacgt attacctggt
     8461 tgctggataa ccaaaaaaga cttgtcgtac ctgactggga atcatcggca acctgtatct
     8521 cggcaggatt atgcataggg atggtgccaa cacatttcgc caaaccgtgg cttaatgagg
     8581 ggaagtgggt agcacttgaa ctggaaaacc ccttcccgga ttcggcatgt tgtctgacat
     8641 ggcagcaaaa tgatatgtcg cctgcgttaa cctggctgct tgagtatctc ggcgatagtg
     8701 agacgctgaa taaggagtgg ctgcgggagc cggaagagac tcccgcaacg ggtgattaac
     8761 gacgatagtc gcggaacggg ccgtcagcca cggagcggcg ttcaatcaaa cgcggatgca
     8821 cttcaatgga ctgcggttct tcacgtttgt tgacgatacg atccaacagc atgttgaacg
     8881 ctgtttcacc cagcgaatct tttggctgat ggatcgtggt cagcgccggc gtaaaatagc
     8941 gcgcgttgcg cacgttatca taaccgatca gcgaaacatc ctgcgggacg cgcaggccca
     9001 tttcatcagc agcacaaagt gcgcccattg ccatgatatc gccaccacag aagacggcag
     9061 tagggcgatg cggctgcgac aggatttgct gcatggcgcg ataaccggat tcaggttcaa
     9121 agtcaccctg cacaatccag ctttccggca ccttgatcat cgcttcttcc atcgccttca
     9181 taaaaccggc aaggcggcct gcgccggtgt tacgttccag cgggccgggg atgacgccga
     9241 tttcgcggtg accgcgttca atcagataac gcccggccat gtagccgcct tcgaacgcgt
     9301 tatcaatgac cgcatcggtg aagtcagctt ttgcttcacc ccagtccatc accaccattg
     9361 ggatatggcg atactcttcc agcatcgcca gcaacggctc tgggtactca gaacacatca
     9421 ccagcagacc atcgacgcgt ttttgcgcca tcatcgacag ataagcccgc tgtttctcaa
     9481 gattgttcca cgcattaccc agaatcaggg tgtaaccttt ctggaagcaa tttttttcaa
     9541 ctgcttcaat gatctcggca aaataggccg cttcgctgct ggtcgccagc aaaccgatag
     9601 acttggtgtg gttaaccttc aggctacgcg ccaccgcgct aggggagtag tgtaattctt
     9661 taatcgctgc ccacacggcg ttgcgcgttt cttcagcgac gaaacgtgtt ttgttgatca
     9721 cgtgtgacac agttgtagtg gaaacgtttg ctcgtttcgc tacatctttt attgttgcca
     9781 ttccatttca ctccagaccc taacttcagc tcctgcaaaa tcgcaaggta aacgtttgcc
     9841 tttacacacc ttacgcacaa caaaagtgtt gcggtacgcc ggaaaaacga cacggagcgt
     9901 caggaagggg tcaatggcca gtacgcaaat agtgtaagcg tggaattttg gccgatcttg
     9961 acgaaaaggg gaagtggtaa aaacacttat cattataaat gacgggagat tttttcataa
    10021 cagtgtgtaa aaatgcgtaa tatcactttt tgtgtggtaa taaaaaagga gaaaacttga
    10081 tgagcaccga ccttaaattt tcactggtaa caacgattat cgtcctcggt ttgatcgtag
    10141 ccgtgggttt gactgccgcg ctgcactgat ttcctctcga gggagcgcgt tctccctcgc
    10201 atctccttcc tgtttgttag caaaatggca aaagtgtttt ctctttttgt tattttcatt
    10261 tttttgtgat tgctgtcatg ctttcgccac tttaatgaat atcgcgtaat gacgcgaaga
    10321 ggtggagata aggcatgaaa attaactatc cgttgctggc gctggcgatt ggcgcgtttg
    10381 gtatcgggac aacggagttc tcgccaatgg gcttgttgcc cgtcattgcg cgcggtgtgg
    10441 atgtctcgat tcccgctgcc ggaatgttaa tcagtgccta tgcagttggc gtaatggtgg
    10501 gcgcgccgct gatgacgctt ctactttctc atcgtgcccg ccgcagtgcg ttgattttcc
    10561 tgatggcaat tttcacgcta ggcaacgttc tttccgccat cgcgccggat tatatgaccc
    10621 tgatgctttc acgtattttg accagcctga atcacggagc attttttggt ttgggttcag
    10681 tcgtggccgc aagcgtggtg ccaaaacata aacaggccag cgcagttgcc actatgttta
    10741 tggggttaac cctggcaaat atcggtggcg tgccggcggc gacctggttg ggtgaaacca
    10801 tcggctggcg gatgtcattt ctggcaacgg cggggctggg agtgatttca atggtaagtc
    10861 tgttcttctc attacctaaa ggtggtacag gggcacgacc tgaagtgaaa aaagagctgg
    10921 cggtattaat gcgtccgcag gtgctgtctg cattgctgac gacggtactg ggagctggtg
    10981 caatgtttac cctctacacc tatatctctc cggtactgca aagtattacc cacgcaacac
    11041 cggtgttcgt cacggcaatg ctggtgctga ttggtgtcgg attctctatc ggtaactatc
    11101 tcggcggcaa actggcagat cgttcagtta acggcacgtt gaaaggcttt ttgttgctgc
    11161 tgatggtgat tatgctggca atcccgttcc tggcccgcaa tgagttcggc gcagctatta
    11221 gcatggtggt gtggggcgca gcaacctttg cggttgtacc tccgttacag atgcgcgtga
    11281 tgcatgtcgc cagtgaagcg ccaggtctgt cttcatcagt caatattggt gcctttaatc
    11341 ttggaaatgc gctgggagca gctgctggtg gtgcggtaat ttccgctggg ctgggataca
    11401 gctttgtgcc ggtgacgggg gcgattgtcg cgggactggc attattgctg gtgtttatgt
    11461 cagccagaaa acaacctgaa acagtttgcg ttgctaacag ctaattaaac aaaaacgcag
    11521 aaggataacc ttctgcgtta cagttgctga caacgtgcga tttatcttgc cgaatgcgct
    11581 gttaacgcct catccgcgtt ggcatggtaa gcgaagcgca tcaggcaatg ctgcatttgc
    11641 catcagttat tatgcagcga gatttttcgc tacgaattcc cagttcacca gcgcccagaa
    11701 gtgctccaga tagccaggac gtgcattgcg atagtcgatg taataagcgt gttcccagac
    11761 atcaacggtc agcagcggag tcgcatcggt ggtcagcgga gtacccgcgt tagaggttga
    11821 aacgatagcc agtttgccat cgctgttttt caccagccag gtccagccag aaccaaagtt
    11881 tttgatcgct gcatcagtaa actgcgcttt gaaatcggca aagctgccaa aagatgcggc
    11941 gatagcttca gcgacttttc cagtcggttc gccaccggcg ttcggtgcca ggcagttcca
    12001 gtagaaagta tggttccaga cctgagctgc gttgttgaat acgccacctt cagagctgcg
    12061 aataatctct tccagtgatt taccttcaaa cgcggtacct ttaatcaggt tgttcaggtt
    12121 agtgacataa gtctgatgat gcttgccgta gtgatactcg atagtttccg cagaaatgtg
    12181 cggtgccaga gcatctttag catatggtag tgcaggtaat tcgaatgaca ttgctactct
    12241 cctttattat taatttgcat acgtacaata gccttattgt gcgtatggat agggtagcaa
    12301 attaaaaggt gagacaaaag atgaacttac cctgttgccg taacaacagg gtaaagtttt
    12361 agcgaagtgt ttttggggtc attacccgac gagcgccaac atagtggcgc tgccagtagt
    12421 cttcactgag agaagtgatt tgaatttcct gacctgtgcg cggtgactga ataaatttgc
    12481 cgttgccgac atacacgccg acatgatcgg ctgtaccgcg tccctgagta cggaaaaaga
    12541 ccaggtcgcc gtttttcagc tcactacgtt cgataggtcc tgcatcacgc aggtgataca
    12601 tttcattcgc cgtacgcgga atacgaattt tcaccaaatc tttataagcg taataaacca
    12661 ggccgctgca atcaaaaccg gtacgcggtg agctgccgcc ccaacgatat ggcttaccaa
    12721 tttgctgcat cagtttattc attgccactt tcgtggcttt ttgcaccttc gctttgtgcg
    12781 cgtcggcaag ggttcctgac gcggcatttt tgactttcac acaatgcgat ttatagccct
    12841 tacgtttggt acacttttcg gtaaagctgg ctgaggccgt tttattgacg gcgtgtttgc
    12901 tgcgagtttt ggcgttttta gaagcggtaa tggaagattt tttaacggaa gaagcggttt
    12961 tgcttttggt cgtactttta ctggcggctt ttttcgctgt tttctggctg gctttctggg
    13021 tctttttggt ggttgccgtg cttttctttt tatctgcttt tttggtgata tgagcggtag
    13081 cagaactctc cctggcttgc tttgaagcat gggccatagg cgttaaaggc agggtggtaa
    13141 aaagtaaagc acagagcgtg atcgaaatac ggtttatccg cgccactgag cgtccctctg
    13201 ttaaggcagg tctatcatgc ctgcgtctga tttacaaaac ttgatgattc taatgcatag
    13261 aagaagagaa agttaatagc ttttattgtt aaaaaatacg tttcgttggc gagcgcaaaa
    13321 aaatgcagca gtgaaagcta aaaatggtta taaatacgcc agtttgccgc gcatcagaga
    13381 agaaacgaat atttgaaatg caacaaatag agcaaaggga taaaatagca aaagcgtgat
    13441 gaaaatgtta ttttccgatg ccgcactgac ccgctacaat gtcagattac tgccgtaaag
    13501 aagtttgagg aagcaagaca atgagcacca ctatcgaaaa aatccaacgc cagattgctg
    13561 aaaacccgat cctgctgtac atgaaaggtt caccgaaact gccgagctgc ggtttctctg
    13621 cccaggcagt ccaggcgctt gccgcatgtg gcgaacgttt tgcctatgtt gatattctgc
    13681 agaatccgga cattcgtgcg gaactgccga aatatgctaa ctggccgacc ttcccgcaac
    13741 tgtgggttga cggcgagctg gtcggcggtt gtgatatcgt gatcgaaatg tatcagcgtg
    13801 gcgaactgca gcagctgatc aaagaaactg ccgctaaata caagtctgaa gagccggacg
    13861 cggaataatc cgtcctgcaa tactgacgga tgcgaacggg catccgtcag tcctttatcc
    13921 ggtgcgccgt aaaacctcgt ccttcagggc ggggatataa ggcgcggttt tccacctgac
    13981 caggtgtttg ctgttgttca atgtattggc ggatgacgga tattggcgca ccttcgcagc
    14041 tactggcaaa atagccagga ctccacaaaa cgcctttgta gtaatacctg actgcaatat
    14101 ctggtcgatc tcgtcgcagt aacctattcg atatgccttt gaggctgttt accagactgg
    14161 atatcgccag tttgggagga tagttaatta acaaatggac gtgatctggt tcgccatcca
    14221 tttcaaccag ttcagctgca aaatcagcac atacatttga aaagtaagtg cgtagttttt
    14281 ctgtcgtgtc atggtcaaaa atctggcgtc ggtatctggt gacaaagacc aggtgaacat
    14341 gcatcaggaa aacacaatgc cttccacgcc ggatatcggt ttcttttttc acagaccaaa
    14401 gtatcatttg acctgtgaaa cgattacagg catttaaatt ccagttaaga cccggtggtc
    14461 aacaggagcg tgaaatgagg cgcttcgcag gcgcatgtcg tttcgttttc aatcgtgctc
    14521 tggcgcttca gaatgagaat catgaggccg gaaataaata catcccttac gggaaaatgg
    14581 cttcctggct ggttgagtgg aaaaacgcca ctgaaacgca atggcttaaa gattcgccct
    14641 cacagccatt gcaacagtca ctgaaagacc ttgagcgggc ttacaaaaac ttcttccgga
    14701 agcgggctgc ttttccccga ttcaaaaagc ggggacagaa tgctgcattc cgctacccgc
    14761 agggcgttaa gctcgatcag gaaaacagcc gtatttttct gccgaaactt ggctggatgc
    14821 gctaccggaa cagccgtcag gtcacgggag ttgtgaaaaa tgtcactgtc agccagtcct
    14881 gcggtaagtg gtacatcagt attcaggcag aaagtgaagt atcaactccg gttcaccctt
    14941 cagcatcaat ggtcgggctg gatgctggcg tggctaaact tgcctcgctg tcagatggca
    15001 cagtctttga gcctgtaaac agttttcaaa aaaaccagaa gacgctggcg agacttcagc
    15061 gacagttaag ccggaaggtc aaattcagca acaactggca gaagcagaaa cgcaaaatac
    15121 agcgactgca ttcctgtatc gcaaatatcc gcagggacta ccttcacaaa gtcacaacga
    15181 ccgtcagcaa aaaccacgca atgatagtca ttgaggattt gaaggtcagc aacatgtcaa
    15241 agtcagcagc gggtacggtc agccagccgg ggcgcaatgt ccgggcaaaa tcaggtttaa
    15301 accgttcgat actggatcag ggctggtatg aaatgcgccg ccagcttgag tacaagcagc
    15361 tctggagtgg cggtcaggtg cttgctgttc cgccagcgta cacaagccag cgttgcgcgt
    15421 gctgtggtca tacagcgaaa gaaaaccgcc tgtcacaaag taaattcaga tgccaggtat
    15481 gtggatatac agcgaacgcc gatgtaaatg gcgctcgtaa cattttagcg gcggggcacg
    15541 ccgttcttgc ctgtggagag atggtgcagt caggccgctc gttgaagcag gaacccaccg
    15601 aaatgattca ggcgacagct tgaacgtagc agggaaccac gtctttcagg gcgtggagga
    15661 tgtcaactat ccccaatcca gcccttgtgg cgaacttgaa aatcccgcct cacgcagcag
    15721 cgtaaacatc ggcgtttgcc ggaccggaag atcattcact tctgttagcg taaagcgcag
    15781 ccttggttcg cgacgcagtg cggtggtcag cgcctggaaa atctctgggg cgagtaattc
    15841 ctccttttcc tgccagacca gcattttttt gccgccttgc ggcagataga gtaacaattt
    15901 gccgccagaa acgaccacca gcgcacccgc ccgacgcgtt ggtaccagcg ctgccggatg
    15961 tgcaggccag ggcagaagat ttccccacac attagccgga tcgttggcgg agagcgccac
    16021 tggtgtatag tggcgtgttt gcgtggcttg tgtcgccaga tcgcgcaatc ggtcaatagt
    16081 cagacgttca gcgaattgcg cgccacccag accttctaca aaacgacctc gcataattcg
    16141 cccggagtct tccatacttc gacaaagtgt ttgcatcgat ggaaacccgc cagggatatt
    16201 ttcggctatc accgcctgac gactgatgat gccgtagcgg tcgagcatat tttccgccag
    16261 cgccagcatc ctttcggtat cgtttagtgg ctccacctgc aataacgacc agcgtccagc
    16321 cagatttggt gtgttgtaag atacccgcgg cgagacgggt tgtgcataga caggacgccc
    16381 acgacgagcc cggtgactgc ggcgagttga ggtgcgtgcg ttggaactgc tgcgggtgag
    16441 ggcgcgtaac ggtgcccaaa tatcgctggt aatgacgcct tgccagacca gcgcccataa
    16501 cgcctcttgc agtgccgagg aatcaaccga ttcgccgatt ttatcgcgta tccgttggct
    16561 gatttgttgt gcaaaccagg ctcctccgtc agccagaaca gcgattatcg cttgctgcag
    16621 cgccgaacga ttcgcctgat ccgcttcggc gggagtgaac gattctgcgg catatttctg
    16681 tagatgcagt gccaccaggc cgtcatcttc acccagcttt tttttgtccc gaccagataa
    16741 ccgctccggt tgccagtaat tcatcgagca tttctggcga atagtcgcgt acgcgggcag
    16801 gcaggatctg gctttcccag agtgaggcgg gtaaaccgac tccggcaagc tgttcgatca
    16861 cccgcatcac gccatctacg ccctcataaa cgcctggcga tgttgaggca aagagcgccg
    16921 ggctaccatc ggtggcgggt aatacgccct gacgttccag cagcaatcgc gcataggtcg
    16981 tggctgcaac gggacgcgtc gcttctctgg cggcttgcag cgagcgcaaa cgcagacgac
    17041 gaaatacttc atcgctcacc cagatgtctt gttgcagatt catcaccaga ccctgttcac
    17101 gcagttgctg aagctgctct tcgacaatgg caataccgag actaaactca tgagccagtt
    17161 gttcagccgt gaccagagca tgagcccgga gatagcgcag aaagaggtcg cgaagcgggt
    17221 aactgactct atgtaaataa atctctggca atgactctgg tagtcgtacg ccgagggcat
    17281 cacgcagcct ggcggcatca tccatacagg caagacgctc ctgtccgcta atcatcgcag
    17341 ggaagattcg ttttactgca agaagatttt ccagatacga cgcaacctct tcactgctgc
    17401 ctgtatgccg ttgcgccagg tcttcaacgg tcattggccc cagttcgcgc agcaggtcga
    17461 acaatccttc ttcacctttc gctcttctgc caggagccag tcgttgcaac tcttcttcca
    17521 cctggcgaat gacctgcggg tcgagtaatt cccccggatc gacctgtccg agtagattgc
    17581 gcagtaactc gctgtccagc gacagtacgg atgcccggcg ctctgccagc ggggcgtcgc
    17641 tctggtacat aaattccgcg acatagccga acaataaact tgtggcgaaa ggcgagggcg
    17701 tagtggtcgt tatatcggat atttgaattt cgccaccgtt caggcgacgc atcaaacgtt
    17761 ccagtgcggg aagatcataa acatcttgca gacattcgcg tagggtttcg agaatgaccg
    17821 gaaaatccgg atatccctga gcgatttcca gcaactgact ggcgcgcagc cgttgttgcc
    17881 aaagcggggt gcgatggccc ggcgtgcggc ccggcattaa taatgcccgc gcggcgcatt
    17941 cgcgaaaacg ggcggcgaaa agtgccgagc tgcctaccgc ctcgcggaca atttgcagca
    18001 acttttctgg ttcaaacaaa aaaatcgcgg cgtcgggcaa tttaccatca gtgtcaggaa
    18061 tgcgcgcaac aatgccgtca tcactggcga ccacagacgc gtcagcgccc catagggcat
    18121 gtattcgccc ggcaatcgcc agcgcccagg gttcatgcac ccgtcttcca tagggagagt
    18181 gcaaaataat acgccagtcg ccaatttcat cacggcaccg ttcgaggacc agatggcgac
    18241 tccccggaac gatgcccgtc gcgttgcgtt gctcgtcaat caacccctga atattggcgt
    18301 tcgtattttc ttctgccagc cacgggggaa tagtcccgga aaagaacgcg ccatccgcca
    18361 gtaaatgaag aaaatcgccg atcatctcgc ctaattcagc cggacgtccg ttaccttcac
    18421 cacgccagaa ggggagccgg gcagaacgac ccggagcagg agtcacaatc acctgatcgc
    18481 gggtgatttg ctggatccgc catgaggtag cgccgagagt gataatgtcg ttcacccgcg
    18541 actcatatac catctcctca tccagttcac ccacccgccg cgaaccggcc ttttcttcac
    18601 cttcgggtaa taacacgcta tacatgccac gatccggaat ggtgccgccg ctggtaaccg
    18661 ccaacaattg agcgccaggt cgggcggtca atatcccggt ctccctgttc cagaccagtt
    18721 tggggcgaaa agcagaaaaa tcgccagagg gatagcgccc ggaaagcata tccagcgtgg
    18781 cgtcaaaaac acgtcttggc agatctttcc acggtgcggc acggcgtacg cgggagtacc
    18841 attcgtctac ctgtaatgca tccatcgccg cggcggcaac ggtttgctgc gcaaggacgt
    18901 cgagaggatt atgcggtggt gtcaggtttt ccagcctgcc tgcgaacata cactctacaa
    18961 tgactgcgga atcgactaaa tcacgccggg tacgggggaa aaacagccct ttagatacac
    19021 cgccaacctg atgtccggcg cgaccaatgc gttgtaaccc actggcaaca gaaagcggcg
    19081 ttgccacctg aatcaccaga tccaccgcgc ccatatcaat ccccagttca agactggagg
    19141 ttgcgaccac gcagcgtaat tcacccgatt tcagcgcctg ttcggtgatt gctcgttgtt
    19201 ctttagagac ggagccgtgg tgtgagcggg caataaaaac gtcgctactt tgtacacggt
    19261 tagaggttgc gccggaggtc gactcgaaat gggccgcatc aacggcgata gacggggaac
    19321 gctgtaagcg tgcggcgtaa agctcattta atcgtgccgt cagtttttcc gccagcccgc
    19381 gtgaattagt aaagacaatg gtcgagcgat ggcgcaacac ttcatcaagg atacccgttt
    19441 caatatatgg ccagatggag ccttcccggc cggcatggct gtcttcgccg gtgccgctgg
    19501 cgaccgatga gacatcatcc atattggcga ccggtacgac aattcgtatc tgcggatggc
    19561 gcattgcggg cgggttgact accgtaaccg ggcgatcgcc accaagaaat gctgccacat
    19621 cgctggctga gcgcacagtg gcagaaaggc caattcgctg tgctgaggtg tggagcagcg
    19681 catcgagccg ctccagactt aacgccagat gcgcaccacg tttactgccc gctaccgcgt
    19741 ggacttcatc aataattacc gtttcgacgc cgtgtagcgt ttcgcgcgcg cgggaggtca
    19801 gcatcagata gagtgattcg ggtgtggtga tcagaatatc cggcggatta cgggtgagtt
    19861 tgctgcgttc ctgtgcaggc gtatcgccag tacggatccc tacgcgaaga ttgacttccg
    19921 tttcgccgcg ccgccgccgt tcatcggcaa tacccttcaa caggatctgc aagttgcgct
    19981 gaacgtcggt gcccagggct tttatcggtg aaatatagag gatgcgtgag gttttacgct
    20041 tatgcgcctc gcgggtatct tcgccgcctt cgcggaagag ccgatcgagg gcgtagagaa
    20101 atgctgccag cgttttcccg gagccagtcg gtgcaatcac cagcgcatgt tcgcctcgcg
    20161 ccgccacatg ccaggtttgc gactggacag cggtcggctg tttaaaggcg cgaagaaacc
    20221 agtcgcgggt cgccggtgaa aacacgtccg gcaggagcga tgaagggtct ggattatctg
    20281 ccattaacgc tattccccgt gaatatcatg cttgatgcgt ggaatagcct cactatagat
    20341 gcctgaatca ttgcatgtca tcaggcatcg actcgattac acctcttcgg cggcaggtag
    20401 cggccagcct cccagacgtt tccagcggtt gacgatttca caaaacagca cagcagtgcg
    20461 ttcggtgtcg tacagcgcgg agtgcgcctg ggtgctgtcg aagtccatgc cagcggtctg
    20521 gcaagccttt gacaatacgg tttgtccgag tgccagcccg gccagtgcag cagtgtcaaa
    20581 agtggcgaaa gggtggaacg ggttacgttt cagcgaggcg cgttctgcgg cggccatcat
    20641 aaagctgtga tcaaaattgg cgttgtgcgc caccataatg gcgcggttac agccgctcgc
    20701 tttaatacct ttacgtacaa ctttaaaaat ttcgtgcagc gcctcgtatt cgctgaccgc
    20761 gccgcgatcg ggatcgttcg ggtcaatgcc gttgaaggcg agggcttctg gttgcaaatt
    20821 tgcgccgaca aatggttcga cgtggaaatg taatgtggtg tccggcatca gccagccttg
    20881 ttcatccatt ttcagggtga tggcggcaat ctcaagcagc gcatcggttt tggcgttaaa
    20941 tccggctgtt tcaacatcga tcacaacagg ataaaaacca cgaaaacggt cgcacagacc
    21001 ggtaagttga gcgttatcgg acatctgatt ctcttaatac ggataaattg cagcgcgcat
    21061 tatgacaaat attacttgtc gatgcagtaa agatgcgggc gcgatgagtt cacgcccggc
    21121 aggagattag ttgcccagac cgcgaccggc gtctttctct tcgattaact caattttgta
    21181 accgtccgga tcttccacaa acgcgataac cgtagtaccg ccttttaccg gacccgcctc
    21241 acgggtcacg ttacccccgt tttgacggat tttttcgcac gcttcagcgg cgttatctac
    21301 gctaagcgcg atgtgaccat aagcagtgcc gagttcgtat ttatccacgc cccagttgta
    21361 ggtcagttca atcaccgctt cttcggtttc cgggccgtag ccaacaaacg ccagtgagta
    21421 tttgtattcc gggttttcgc tggtacgcag cagtttcatg cccagcactt tggtataaaa
    21481 atcgatggag cgttgcaaat cgccaacgcg cagcatggta tgaagaagac gcatcttttt
    21541 atcctcaaaa tggctaacag attcaaaatg ttgttttagt atagcggcgg ctttacgccg
    21601 ctcgcaatgt tggattacaa cgtcgggtaa tcggtatagc cttccgcgcc gccaccgtag
    21661 aaactttcgg cacgctgtgg gttaagctca gctttgagct gcaagcgggc gaccagatcc
    21721 gggttcgcaa tccagtcacg accaaatgcc accgcatcaa ttaacccttt gccgatcagc
    21781 gtttcagctt tttctactgt gtatgcacct gcgccgataa tcggaccgtg gaaacgggcg
    21841 cgtacttttt cgcggaacgc atcagtatac ggttcacccc ccgcccaatc tggttctgac
    21901 atatgcagat aagcaatgcc gcgtttaccc agttgttcaa tcagatacag tgcatcggct
    21961 tcttcattcg ggccgttatc tgtgttctgg aaagtaccga ttggcgaaac gcgaatgcca
    22021 atgcgatcgg caccccattc ttcaatcccg gcatcgacca cttccagtac caaacgtgcg
    22081 cgattttcca cgctgccgcc gtactgatcg gtacgatggt ttgaagaagg ggaaaggaac
    22141 tgatgcagca aataaccgtg agcagagtgg agctctacca gatcaaaacc ggcttcacgc
    22201 gcgttagcaa tggcctgacg gaaatcattg acgatacccg gaatctcttc cagttcaagc
    22261 gcacgcggca tggatgtttc aacgcggatc gcccgaccat tttcatcgcg cagagaagta
    22321 cgtgttcccg cgctaagtgc tgaaggcgct accggtgcct gaccgccagg ttgcaggctg
    22381 gcgtgagaaa tgcgtccggt gtgccacagc tgcaaggcca tatgaccatt ttcagcatga
    22441 acgccagcgg tgattttttt ccatgcggca atttgctccg gactatggat gccaggcgca
    22501 cctgcatatc cttttgcctg ggcagaaatt tgcgtggctt cactaataat caaaccggca
    22561 ctggcacgtt ggcgatagta ttccgccatc aacggggtag gaatgtcacc cggttcaata
    22621 ctgcgcagac gcgtcagcgg tgccataaaa atacggtttg ccgccgtgat cgcgcccact
    22681 ttcagtgggg aatacagttt ttcagatgac ataaaggctc ccggtcgtct ggtgataaaa
    22741 aatgcctgct aaacggcagg cgtcgcaata atgtttttta catgggccag cgcgttttcc
    22801 agtggctcga aactgcgcga aattttggcc tgcagattcg cgccaagcca tagtgcgtaa
    22861 agcacttgtg cctgttgtag cggttcgcca caaaaggtta aacaatggtt ctcacggcca
    22921 ttttccagag cctgcgagag cagggcgatc acgccgcgag cacctttatc catcgcgctg
    22981 cgcatatctt ctgacagatc gcacacttcg gcagagagtt ttactgtcag gcaaccactg
    23041 atggttccat gttggcaaaa ctggttcagt gtttgctggt aataagccag tatgcggtcg
    23101 cggtagttac cttcgccgga ttgcagcaac tcagccagtc gctggtgata tgcggcgtaa
    23161 tgacgctcaa gcatggcaac gccaaacgct tctttagagc gaaagtagtg atagaaggac
    23221 cctttcggca cttcagcggt ttttagtaat tcgcttagcc ccatcccggt gaatccacgt
    23281 tgcaggcaaa gttgctcgcc cgtcgccaag agatgttcgc gagtatcatg ttcggtgtgt
    23341 ttgttcatgc gttggagtgt agtagaccag tcggtctagt gcaagcaaag ttgctacttt
    23401 ggacattaac agcttcaata atggtaataa tcttatctga ggagggcgag gtggcggagc
    23461 aattagagtt ctttcccgtc cagagcccgt gccggggaat ttgccagtct gacgaacgcg
    23521 ggttttgtcg cggctgtttt cgtagtcggg atgagcgttt taactggaat aaaatgagcg
    23581 atggcgaaaa acaagaggtg ctgcgccttt gtcggcagcg attaatgcgc aaattacgcg
    23641 caaataagcc agcgtcatca gacgaaccgg agcaaccatc actcttttga acctaatctt
    23701 gcgtatactc ctctcatatc tgataagagg aagcgattat ggttcagcgt attactattg
    23761 cgccggaagg cccggagttt tcccgttttg tgatgggcta ctggcgattg atggactgga
    23821 atatgtccgc ccgccagctg gtcagtttta ttgaagagca tctggatctc ggcgtgacca
    23881 ccgtggacca tgctgatatt tatggtggct atcagtgcga agcggcgttt ggcgaggcac
    23941 tgaaactggc acctcacctg cgtgaacgga tggaaatcgt cagtaaatgc ggtatcgcga
    24001 cgaccgcgcg tgaagaaaac gtcattggtc attacatcac tgaccgcaat cacatcatta
    24061 agagcgccga acagtcgcta attaatctcg cgaccgatca tctggatctg ctgttaatcc
    24121 accgaccaga cccgttaatg gatgccgatg aagtggcgga cgcgttcaaa catctgcatc
    24181 agagcggcaa agtgcgtcat tttggcgtat cgaactttac gcctgcgcaa tttgccctgt
    24241 tgcaatcacg tctgccgttt acccttgcca ctaatcaggt ggaaatatcc ccggtgcatc
    24301 agccgttact gctggatggc acgctcgacc aactacaaca actgcgtgtt cgtccgatgg
    24361 cgtggtcctg ccttggtggt ggtcgtctgt ttaatgatga ttatttccag ccgctgcgtg
    24421 atgaactggc tgtggtggcg gaggagttaa acgcgggctc gattgaacag gtggtttacg
    24481 cctgggtatt acgtttaccg tcgcagccgc tgccaattat cggctcaggt aaaattgagc
    24541 gcgtacgggc agctgtcgaa gcagaaacac tgaaaatgac ccgtcaacaa tggtttcgta
    24601 tccgtaaagc tgcactgggg tacgacgtac cgtaagcact tttaggaata gccgccgttc
    24661 aaaaatgtgt cactggttta cacttattca ggaatgcaca atgaacggag gtcctatgaa
    24721 acgttttagt ctggccattc tggcgctggt tgttgcgacc ggcgcacaag ctgccagtga
    24781 aaaagtcgag atgaacctcg tcacgtcgca aggggttggg cagtcaattg gtagcgtcac
    24841 cattactgaa actgataaag gtctggagtt ttcacccgat ctgaaagcat taccccccgg
    24901 tgaacatggc ttccatattc atgccaaagg aagctgccag ccagccacca aagatggcaa
    24961 agccagcgcc gcggaatccg caggcgggca tcttgatcca caaaataccg gtaaaattaa
    25021 ccatcggaaa ggcgatgatg gcagccgtgt agcccgccag ttgaaatgca tacgcgacgt
    25081 tattcgtgaa gtgcgcacag gcctaggtac aaaagccaag ccacgccgac atgctcaata
    25141 gaaaaaacca cggctcattg agcgtatgtc cggcaagaag cagtgccgca atggctccca
    25201 gtaaactgcc agcgatgcgt ccgaggcttt tgctgatgac accgccaacg gtgggaaagc
    25261 taaccactgc agccgaggtc atcgcccaat agggttcatc cagattcaaa taataggcaa
    25321 ccgtcagcgc cagacacatg gcaatggtat tgcgtaacgc ataacgccat tgcgccagcg
    25381 tggccctgaa ccagcgcaaa gcggacggga acgcgttggg cgagacgaac ccaggggacg
    25441 ttaggtttca catccggaat aaggcttgag tcactttcaa cgctctgatc ataaatcgcc
    25501 cgaccgatac tggaaacgtg accctgtaac gttttgttat cactatagag ggtaatttgt
    25561 gctggtgcac cttcgcggat atggcgcaac ttggtttctt caaaataacc aatgacgtaa
    25621 aacgagtggc tgtcgacaag ggcaaacagc ggtttcccgg tgtcagcgta atcgccgatg
    25681 cgggtagtga ggttagtcac ccatccactc accggggcgc gaatttccgt ttgcgccagt
    25741 tgccattgtg tctgtttcag cgtcgcctgt gcggcattaa cactggcctg catcgcttta
    25801 acattgaggt tagcggtatc gagttcttcg gcaaaaataa aattttgtga gagatgacga
    25861 cgacgattcg cttcgttatt ggcttttgcg aggtcagatt gcgctttcgc cagctgagcc
    25921 tgtgcgttca gctcggcgat ctgaaagggc gttttatcga tcgtgagcag aagttccccc
    25981 gcgttaacca gttggttgtc ttttatattc agctcaacaa tacgtcccga cacctgtggg
    26041 gtgatagaaa cctgttctgc gtgtattttc ccgtcgcgtg tccacggaga ttgcatgtaa
    26101 taattccaca gccaccatcc ggcaagaacc gcaactatcg ctacaatgat tgttgagaaa
    26161 tacttaattg ttttcattga catagttacc acgcaatcag tattgccaga gcaaggcaaa
    26221 cgcaaatcgc aaacagcgat aaatccatta acaagggatg ccagatgtca ccggcgtaga
    26281 tccagccacg aagcagacgg tgtacgacaa gccagatgac gaatccaaac gcgaatgctt
    26341 tgaaaaacgg aggaaaatag acggacgcac cgaacaccag atcttgtaag ggcaatcctg
    26401 ttgcattgag cataaacttc acaaagtaga ttcctttacg accgaatata ttgcgttaga
    26461 ttaataaata ttctttaagt gcgaaaaatt tacgcgcaat ttctgaaaaa tatgtctgac
    26521 ggtaaccaaa tgcagcaata catttgtttt agcaatacaa ttgctgcaca ctattctaaa
    26581 agccgcataa tatcttagca agctaattat aaggagatga aattggaatc gccactaggt
    26641 tctgatctgg cacggttggt gcgcatatgg cgtgctctga tagaccatcg cctgaaaccg
    26701 ctggagttaa cacaaaccca ttgggttacg ttacacaata tccatcagtt acctccagac
    26761 cagtcgcaaa ttcaactggc aaaagcgatt ggcatcgagc agccatcact ggtccgtact
    26821 ctggaccaac tggaagaaaa agggttaatt tcgcgtcaaa cttgtgccag cgatcgtcgg
    26881 gctaaacgta ttaaactgac ggaaaaggca gagccactga tcagcgaaat ggaagctgtt
    26941 attaacaaaa cccgcgcgga aatattacat ggcatctccg cagaggaact ggagcaactg
    27001 attacgctca tcgcaaaact tgagcataat atcattgagt tacaggccaa agggtgaaat
    27061 gaagggggcg tgtggtcagt aacctgacca cacgcaaact tatttagcgc ggagaaacgg
    27121 tcacctgact gccattgctg gccagtacga cacgttggcc cggagagaaa cgagtgttgc
    27181 cttgtttctg taccaccatg atggtattac catcgtcttt acgaatttcc agctcgacac
    27241 cctgcgtttt gttcattgca ctctgtacgc cctgaccagc tacgccacct gcaacagcgc
    27301 ctgctgcagt agccagagaa cgcccggttc cgccaccaac agtattcccc aggaaaccac
    27361 caagaacagc accgccaatt gcaccgataa cgttggaatc atcaccgccc tgaatctgta
    27421 ccggacgtac gttaacgatg gtgccatagc tgacattctg tacttgtttc gcttcagaag
    27481 cggtataaac atcccctgac agggtgtcgt tattaacaca accgacaaga gacagaccta
    27541 ccattgaaac aaccaatacg cgtttaatca ctgaaaaatc tcctgttcac catgaaacgc
    27601 cacgcaagca tccctcatgg tcaaagtata tggcatatgt gaatccatgg ttacatgttc
    27661 taccaaatat gagcaaatca taatgaaagt aggatcaacc aattataaac aaaacattca
    27721 tgatgattat tgatttgaag ataatgtttt actaccaacg gtaaaaatcg gtcattccgc
    27781 atggtattca cgaaaagttt atgtttgagt ggtgcgcaaa cgtcagattg aaaaaggatg
    27841 aactatgaaa tcgggccgct ttattggcgt tatgtcaggc accagccttg atggtgttga
    27901 tgttgtgttg gcgacaattg atgaacaccg ggtcgcacag ctggcaagct tgagctggcc
    27961 gatcccggtg tctctgaaac aggctgtact ggatatttgc cagggccagc agcttacact
    28021 ttcgcagttt ggacagcttg atactcaact cgggcgactt tttgctgatg cggtcaatgc
    28081 cttgcttaag gaacaaaacc tgcaggcgag agatatagtt gcgatcggct gtcacggtca
    28141 aaccgtctgg catgaaccga cgggcgtggc accacacact ttacagattg gcgataacaa
    28201 tcaaattgtg gcacgcaccg gaattacggt tgtcggtgat tttcgccgtc gcgatattgc
    28261 cttgggagga caaggcgcac cgctggtacc tgcgttccat catgccttgc tggctcaccc
    28321 aaccgagcga cgaatggtgc tcaatattgg cggcatcgcc aatctgtcac tgctcattcc
    28381 tgggcagccg gttggcggct acgataccgg tcctggtaac atgctgatgg atgcctggat
    28441 ctggcgtcag gcaggtaaac cttacgataa agatgccgag tgggcacggg cgggtaaagt
    28501 tattctccca ctgctgcaaa atatgctcag cgacccgtat ttctcgcaac ctgcaccgaa
    28561 aagcaccgga cgcgaatact ttaactacgg ctggctggag cgccatttac gccattttcc
    28621 gggtgttgat ccccgtgatg ttcaggccac actggcagaa ctcaccgccg tgaccatttc
    28681 tgaacaagtt ttgttgagcg gtggctgcga acgattgatg gtatgtggtg gaggtagtcg
    28741 taatccgcta ctcatggcgc gtctggcggt attactgcta ggcacagaag tcaccaccac
    28801 cgatgccgtt ggcattagtg gcgatgacat ggaagcattg gctttcgcct ggcttgcctg
    28861 gcggacgctg gcgggattac caggaaatct gccttccgtc actggcgcaa gccaggagac
    28921 ggtactgggg gctattttcc ccgccaactc gtgatatcag aattaactga attttttctt
    28981 atcgtgtctg ccgttacact tggaagatta aggagggtat ttgccctccg gaccaggaaa
    29041 gtcttcggga tatgactatg aaaaaactgt taattatcat tttgccggtc cttctctccg
    29101 gttgtagcgc ctttaatcaa ctggttgagc gaatgcaaac cgatacgctg gaataccagt
    29161 gtgatgaaaa accgttgacg gtcaaactga ataatccgcg ccaggaagtc agttttgttt
    29221 acgataatca actactgcat ctcaaacagg gcctttcagc ctctggcgcg cgttactccg
    29281 acgggatcta tgttttctgg tcgaaaggtg aggaagcgac tgtctataaa cgcgaccgca
    29341 tcgtcttgaa taactgtcag ttacaaaatc cacagcgttg agatttttcc aggggcggcg
    29401 cacaatagcg tcacccactg acaatccgta aagaaaacca tgtctgataa cgacgaattg
    29461 cagcaaatcg cgcatctgcg ccgtgaatac accaaaggcg ggttacgccg ccgcgatctt
    29521 cccgccgatc cattaaccct ttttgaacgt tggctctctc aggcttgtga agccaaactg
    29581 gcggacccta ccgcgatggt ggtcgctacc gtggatgaac atggtcagcc ttatcagcgc
    29641 atcgttttac tcaaacatta cgacgaaaaa ggcatggtgt tttacaccaa cctcggtagc
    29701 cgtaaagcac atcaaattga aaataatccg cgcgttagcc tgctgttccc gtggcatacc
    29761 cttgaacgcc aggttatggt gatcggtaaa gcagaacgac tttcgactct cgaagtgatg
    29821 aaatattttc atagccgccc gcgtgatagc cagattggtg catgggtttc gaagcagtcc
    29881 agtcgcattt ctgcccgcgg tatccttgaa agtaaattcc tggagctgaa gcagaagttt
    29941 caacagggcg aagtgccatt gccgagcttt tggggcggtt ttcgcgtcag ccttgaacag
    30001 attgagttct ggcagggcgg tgagcatcgc ctgcatgacc gctttttgta ccagcgtgaa
    30061 aatgatgcgt ggaagattga tcgtcttgca ccctgaaaag atgcaaaaat cttgctttaa
    30121 tcgctggtac tcctgattct ggcactttat tctatgtctc tttcgcatct ggcgaaaagt
    30181 cgtgtaccgg caaaggtgca gtcgttatat acatggagat tttgatggca agcagtaact
    30241 tgattaaaca attgcaagag cgggggctgg tagcccaggt gacggacgag gaagcgttag
    30301 cagagcgact ggcgcaaggc ccgatcgcgc tctattgcgg cttcgatcct accgctgaca
    30361 gcttgcattt ggggcatctt gttccattgt tatgcctgaa acgcttccag caggcgggcc
    30421 acaagccggt tgcgctggta ggcggcgcga cgggtctgat tggcgacccg agcttcaaag
    30481 ctgccgagcg taagctgaac accgaagaaa ctgttcagga gtgggtggac aaaatccgta
    30541 agcaggttgc cccgttcctc gatttcgact gtggagaaaa ctctgctatc gcggcgaaca
    30601 actatgactg gttcggcaat atgaatgtgc tgaccttcct gcgcgatatt ggcaaacact
    30661 tctccgttaa ccagatgatc aacaaagaag cggttaagca gcgtctcaac cgtgaagatc
    30721 aggggatttc gttcactgag ttttcctaca acctgttgca gggttatgac ttcgcctgtc
    30781 tgaacaaaca gtacgatgtg gtgctgcaaa ttggtggttc tgaccagtgg ggtaacatca
    30841 cttctggtat cgacctgacc cgtcgtctgc atcagaatca ggtgtttggc ctgaccgttc
    30901 cgctgatcac caaagcagac ggcaccaaat ttggtaaaac tgaaggcggc gcagtctggt
    30961 tggatccgaa gaaaaccagc ccgtacaaat tctaccagtt ctggatcaac actgcggatg
    31021 ccgacgttta ccgcttcctg aagttcttca cctttatgag cattgaagag atcaacgccc
    31081 tagaagaaga agataaaaac agcggtaaag caccgcgcgc ccagtatgta ctggcggagc
    31141 aggtgactcg tctggttcac ggtgaagaag gtttacaggc ggcaaaacgt attaccgaat
    31201 gcctgttcag cggttctttg agtgcgctga gtgaagcgga cttcgaacag ctggcgcagg
    31261 acggcgtacc gatggttgag atggaaaagg gcgcagacct gatgcaggcg ctggtcgatt
    31321 ctgaactgca accttcccgt ggtcaggcgc gtaaaactat cgcctccaat gccatcacca
    31381 ttaacggtga aaaacagtcc gatcctgaat acttctttaa agaagaagat cgtctgtttg
    31441 gtcgttttac cttactgcgt cgcggcaaaa agaattactg tctgatttgc tggaaataat
    31501 gcattaagtg gaaaggggga gtgagaaatc actccccctg gtttttatac agggaacatg
    31561 atgaaaaata ttctcgctat ccagtctcac gttgtttatg gtcatgcggg taacagtgcg
    31621 gcagagtttc cgatgcgccg cctgggcgcg aacgtctggc cgctgaacac cgttcaattt
    31681 tctaatcaca cccaatacgg caaatggact ggctgcgtga tgccgcccag ccatttaacc
    31741 gaaattgtgc aaggcattgc cattgccgcc attgataaat tacacacctg tgatgccgta
    31801 ttaagtggct atctgggatc ggcggagcag ggtgaacata tcctcggtat cgtccgtcag
    31861 gtgaaagcag cgaatccgca ggcgaaatat ttttgcgatc cggtaatggg tcatccggaa
    31921 aaaggctgta tcgttgcacc gggtgtcgca gagtttcatg tacggcacgg tttgcctgcc
    31981 agcgatatca ttgcgccgaa tctggttgag ctggaaatac tctgtgagca tgcggtaaat
    32041 aacgtcgaag aagcggttct ggcagcgcgc gaactcattg agcaagggcc acaaattgtg
    32101 ttggttaaac acctggcgcg agctggctac agccgtgacc gttttgaaat gctgctggtc
    32161 accgccgatg aagcctggca tatcagccgt ccactggtgg attttggtat gcgccagccg
    32221 gtaggtgttg gtgatgtgac gagcggttta ctgctggtga aactgcttca gggggcaacg
    32281 ctgcaggagg ccctggaaca tgtgaccgct gcagtctacg aaatcatggt gaccaccaaa
    32341 gcaatgcagg aatatgagct gcaagtggtg gctgctcagg atcgtattgc caaaccagaa
    32401 cattacttca gcgcaacaaa gctctgaaat atctcatttc ggcccacaac gacctgtggg
    32461 ccgatggcta ttactttaag ccttccgctg acagcgcgtc ttgtacttcc ggacgttcag
    32521 ccatacgttg cataaatgcc gcaatgtgct ctaacccttc cagattcagt ttcaccgcgt
    32581 atgcccagcg cagaaccgta aacagatagg catcagcaat tgtaaatctt tgcccgcaga
    32641 tccagtgctc atccttcagt gcctcgttca catattgcag cttcttatcc agctgcgcgc
    32701 gaactgtcgg tttgtactct tccggtgtat ctgggcgaaa cagaggtgtg aaacctttat
    32761 gcagctcggt ggcgatgtaa ttcagccatt cgatggtttt atagcgggaa atactgttta
    32821 ccggtgccag caactggcgg tcggggacgc tgtcggcaag atactgcata atcgcaacgc
    32881 cttccgtcag caaagtacca tcgtccagca gcaatgcagg cacctgcccc ttagggttaa
    32941 cggaaaagta atcgtcaccg ttttcgagac gttttttcat taaatccaca ctgacgagcg
    33001 taaaatcctt tccgctctca cgcagggtaa tatgggaagc gagagagcag gcgcccggtt
    33061 tgtagaacaa tttcatcggt aactcctttt gtctgtggtt taaagtatgg tagtgcgttg
    33121 ctgaacaaaa aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat
    33181 tttacgctac agtagctttc gccgctttgt ctgcagcgtc atcctgcgtc atgcggtgca
    33241 gtttaggcgc ggtcagcagc atcagtactg caataacggc agtagcgaca ccaatctgca
    33301 agaatacgcg accatagact tccagtgaca tcagcgggtc ggtaacgtta tccggcacag
    33361 ccatcatacc cgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc
    33421 tacccataat gaagcccatc agacgctgcg gaacgagttg tgcaaccatt gccagaccca
    33481 gaccagagat catcagttcc ccgatgctct gcaggccata gcttgcgacc agccagctga
    33541 cagacacgat acctgcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac
    33601 cagaacacat caccatgccg attgcaaact tggttggcat cggcagggta tcacccatct
    33661 tgttatagat agcggccaga atcggactac cgatgatgat ccagaacggg ttcagtgcct
    33721 gatactgctc aggttctacg gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa
    33781 agaagttcag tgacgttggc atctggctgt acagcacgaa gaagatgatt gcttcgagca
    33841 tcaggatgaa ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaagcttctt
    33901 taccgaagat aaccacaata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct
    33961 gattgtgcag tagccaggtg gcaacagcga tcagcgccac aacaccaata atggtcagca
    34021 acaggttacg gtagttgact ggttcaaagt ctggttttga accgtactgt ttaacccagc
    34081 gttggcagaa ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg
    34141 caacactcca gccgtatttc gcggccagcc acggggtggc tatcatagag aagaaagagc
    34201 cgatgttgac ggacatgtag tacatggtga atgcaccgtc cagacgtggg tcgtttttct
    34261 catagcatgt agaaagcaga gaagacgggt tagctttaaa caggccgtta ccgaccgcaa
    34321 tagccgccat acccatataa acaataccgg cgtcgtgacc agaccaggca accagagcat
    34381 aaccaatcgc cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt
    34441 cacctaacca gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa
    34501 gggtgattga atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc
    34561 cttgtaggcc gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg
    34621 ccttcggttg tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg
    34681 acacttttac ctcttttttt acatcccata ttaacggggg tgctttctcg cgttatgtct
    34741 aaaaaacata cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct
    34801 tgcactttca gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca
    34861 accattccgg gaaatgttat ccagcgttaa atttttcaaa aaacgtaacg gcagattttt
    34921 tcactgcatt gagaggggga tgatggcttt acggagatat gttctgctat ttagcgtttt
    34981 tcctagtgag atagtccagt ttctgaaaaa tagccagtgt aatgttttgc aggtcaatat
    35041 ttgttgcgtg tgtaaattta aatcatttta acctgattct tgcaatgttt ttacattgcc
    35101 acagcatcgt gatcttgatc acgaatgaat agaaaatttc gctattaaaa aaacaattaa
    35161 cctgcatttt tggttatccg caaacggatt acctgatgat aacgctaagc aatggcatca
    35221 ttgacgttaa aaagatgttt taaaagagga taaagaaagg ttatcaatgg ggtaatcggt
    35281 gttacccctt ttcttcagat gtcaactttc tctttgtatt cacaaagatc ttcaataata
    35341 caagagccac agcggggctt gcgggcaatg caggtataac gcccgtgcag gatcaaccaa
    35401 tggtggcagt cgactttaaa ctctgccgga accactttca gtagcttttc ttctacctgt
    35461 tcgacgtttt tccccggcgc aaattgagta cgattacaaa cgcggaaaat gtgcgtgtcg
    35521 acagcaatag tcggccagcc gaatgcagtg tttaatacga cgttggctgt tttacgacct
    35581 acgccgggca gggcttcaag cgcagcacga tcttccggaa cctcgccatt atgttgttcc
    35641 agtaatatac ggcaggtttt gatgatattt tctgctttgc tgttataaag gccaatcgtt
    35701 ttgatatagg ttttcacccc ttcaacgccc agttcaagca tcgctgcagg cgtattcgcc
    35761 accgggtaga gtttcgccgt cgccttatta acactgatat cggtcgcctg agcggaaagc
    35821 agtacggcaa tcagcaattc aaaaggcgaa ctgaaattaa gctcggtggt gggatgagga
    35881 ttgttctcac gcaggcgagt gaggatctcc agacgttttg ctttgttcat cagacattcc
    35941 ctgtttcacc gtttggcaat gcacgttcgg cagctgcttc agcacggcgc tttttcattc
    36001 tttcatcaat caggtatttt cctgccagca tcagtcccaa gccaataaat gcacctggtg
    36061 gcagcatcgc cagcaggaaa ggggagtcgg tgtggaaaat ctccacgcgt aatacttttg
    36121 cccagctacc taacagcgca tctgcaccgt caaacaacgt gccattgccg ataatttcgc
    36181 gtagtgaacc cagcacgaac atggcgcagg ttgcgcccgt accaattgaa aagccgtcca
    36241 gtgccgaaag cgccggacct tttttggcgg cgaaggcttc agcgcggccc acaacgatac
    36301 agttagtgac aatcagcgga ataaaaatcc ctaatgattg atacaggcca aaggcgtagg
    36361 cgttgatcag catctgtaca gcgctgacca ccgaggcgat gatcatcacg taaatgggaa
    36421 tgcggatctc ggctggcgtc cagtgacgca gcgtcgaaat ggtcaggttg gtcagcgtca
    36481 gtaccagcgt agtcgcaagt cctaaaccca gagcgttagt ggcagtggac gtgaccgcca
    36541 atagcggaca aaggccgagc agttggacca gtgcagagtt gtttttccac aacccctgaa
    36601 caataacgtc tttaatttcg ctcacgcttt attctccaca ggcaggaagt tgagaaagtt
    36661 gtgccggtaa cgtctgagcg tacaatccgg cgcgttttac cgcattaacc accgcgcggg
    36721 gagtaatcgt cgcgccggtg aactggtcga aatcaccacc atctttcttc accgcccagt
    36781 gcgcatcatc cgcaccactg attttttttc ccgcaaaatg ggtgagccag tcagaaaggc
    36841 gcaattcgat tttatcgcca agccctggcg tttcgtggtg ctctgtcacg cgcgtgccaa
    36901 gtaccgtgcc attaaaatcg gctcccacca gcagctgaat cgcaccggaa tagccatctg
    36961 gcgcggttgc ttccagaacg gcggctaccg gtttgtcatc ctgtttggcg atgtaaaccc
    37021 gatgctcacc tttacctaac tctggcgcag ttaccagata gcaactctgt gcgagcgcat
    37081 tgttatagcg ttcggctggc agcacctgac caaataacgc cttttgttgc agactggcct
    37141 gttcagcaat tgtcgttttg gtcatctggt tgatggccgc agttaaccct gtagaacccg
    37201 ctgcaaatag tgccagtgta atgccgtgtt ttcggatagt tttcagcatg gtttaccctt
    37261 tgcgatggcc gtagacgcgc ggacgcgtgt agtaatcgat cagaggaacc gtgatgttcg
    37321 ccagcaggac ggcaaaagcc acgccgtcag gatagccgcc gaaactgcgg atcaaccaga
    37381 ctaataagcc cgccagcgcg ccgaacatca gccgaccacg attggtcgta gaagcagtaa
    37441 ctgggtcagt caaaataaag aatgcgccga gcatagtcgc accagacagc agatgaattt
    37501 gcggtgctgc cagtgtttct ggtgagaaca accagcccaa cgttgcgcat aacgccagcg
    37561 ttactaagaa gctgagggga atatgccagc gaatcgcttt ctgccatagc aaccacacgc
    37621 caccagccag ccaggcgaga tttacccatt gccaaccagc gcccgccaga ataccgctgt
    37681 agatcggata ttgcataatc tgttcaaccg aatgacctgc gcggacagag gttttgaatg
    37741 tatccagcgg tgtcgcctga ctaatgccat caatacctaa gcgtagcgta ttcatatcac
    37801 caccactggc ggtatgaccg ctgaaaataa cctgtatggc gtcgataaaa ccagggatgt
    37861 tgaccgcaat ttcatgtggc ggtaaccagc tggtcatctg caccgggaag gagatcagta
    37921 agaccacata accaatcatt gccggattaa acgggttttg ccccagaccg ccatacaact
    37981 gtttagcgat gatcaccgca aacaccgtac ccagtacgac catccaccat ggcgctaggg
    38041 ggggaatact taccgccagc aataagcctg tcagcaatgc tgagttatct ttcaacgttg
    38101 cggctaccga ctgcttgcgt agtttgagta cgagagcttc ggctaacaga gcactgaccg
    38161 acgccaacag gatctgaacg agagtacccc aaccaaaaaa ccacagttgc gctgcgattc
    38221 ctggcacggc tgcgagcaac accaatagca taatgcgcga tgtctggcgc tggttatggg
    38281 tataagggga gctagctatt ctgaatacca tttagtcctc gtttacaacc ttttgctggg
    38341 cggctttttt ggcctgaacg cgggcaatag ccgcggcaac tgcggctttg cgcggatcga
    38401 cctgttcttc tggttccgca ttagcctgtt gctgttccag cttgcgtgct ttggcgcggg
    38461 caatagcggc ttcgacggca gctttgcgcg gatcaacctg ttcttctggc tccgcattgg
    38521 cctgttgctg ttccagcttg cgtgctttgg cgcgggcaat agcggcttcg acggcggctt
    38581 tgcgcggatc gacctgttct tctggttccg cattagcctg ttgctgttcc agcttgcgcg
    38641 ctttggcacg ggcaatagcg gcttcgacgg cggctttgcg cggatcgacc tgttcttctg
    38701 gttccgcatt agcctgttgc tgttccagct tgcgcgcttt ggagcgggcg atagctgctt
    38761 caacggcagt tttacgtggt tcagcaacgg ttgctgcgtc gttagtttgc tccagttctg
    38821 cctgtttcgc tctggcttgc gctttacggg cttcccgtgc tgcaataatt gcactgttat
    38881 ccgggcgttc gcccgcttta atcacaatag gctgtgtagc ctgggcctgt ttctctttca
    38941 cccgcgccag agcggcagca atcgcatctt tatctttggc tgcaggttga acggctgcgc
    39001 tcttatgtcg ttcaaggcga gccgcttttt cgcgctccag acgagcctgg cgcgcttcga
    39061 aacgcgcttt ggcttctgcg gcgcgctttt cttcctgacg aatagccgca atttcagctt
    39121 tttcctgacg gaaatattgc accaagggaa tattgctcgg gcaaacccac gcgcaagccc
    39181 cacattcaat gcagtcggca atgttatgcg tggtagcttt atcgtgttgc tgacctttgc
    39241 tgaaccagta caactgttgc ggcaaaagat ccacagggca ggcgtcagca caggcgctac
    39301 accggatgca gctttgttct tcctgtggtt cgccaagttc attggcagag ggagccaaca
    39361 gacagttggt aatctttacg accgggacat ccagccatgg caaggtaaag cccattagcg
    39421 ggccacccat aatcaccatt tgatcggcag aggggcagaa tccggcatca ttcaataaat
    39481 gacgcactgg cgttcccagc cgtgcccaga cgttgcccgg gcgagcgatt gcttcgccag
    39541 tcagggttac aacacgctcg gtaatcggct cgccatcaat aacggcacgt ttcactgcat
    39601 aagcagtgcc gacgttttgc attaatacgc cgatatcgga tgaacgcccg ccatgtggaa
    39661 cctgcttccc ggtcagaatg taggttaatt gtttagcacc gccagaagga tatttggttg
    39721 gaatcacccg cagagaaata tcgttagagt ccgccagcac cgcgcgcagc atggaaatcg
    39781 cctgcggttt gttatcttca atgccgataa gaatttcgcg tggctgcaga atatgcgcaa
    39841 gaatgcgaat accctctacg acctgagccg cgcaatcctg catcaaacgg tcatcggcgg
    39901 taatgtacgg ctcgcactca gcagcgttga taatcaacgt ttcaatctta tctccaccac
    39961 cctgcaattt aacgcctgtc gggaatccgg caccgcccag cccggcaaca ccaaactgat
    40021 gtatgcgctc gattaactct tcgcgactgc gagagcgata atcgacccag ccgtcgcgcg
    40081 ggatccagca gtcttcacca tcggcatcaa taatcacgct taattcagct aaagctgaag
    40141 gatgagccgt agagtggggc gcaatagccg taacggtacc cgaggtgggc gcgtgaacag
    40201 gcagcatttt gccgcgacca cgggtaagcg gctggccgcg caatacttta tcgccgacgc
    40261 taacgcacaa ctcaccttca gcgccaatat gctgtttcag tggaataaca aaacgctgcg
    40321 ccagtggtac ctggcgcagg ggggtaccgt tggactgggt tttcatctcc ggtggatgga
    40381 tgccgccgtt gaaatcccag attttatttt ttctgaatgc agagaataac ttaagcatgg
    40441 tgttccacgg gaatgatacg cacgggaatg gtgttcagat cccatttcca ggagtcaggt
    40501 gtttctgcga ccggttgcaa cgagatgcag tgcgtcgggc acggatcaac acataaattg
    40561 cagcccgtac agagatcact cattaccgta tgcatggcac gggtagcgcc aacgatggcg
    40621 tctaccggac aagcctgaat acatttagtg cagccaatac agttattttc atcaataacc
    40681 gccaccatcc gcgcaggcgt tagctcttgc gcttcgccat ccagcggctg cggctcgaca
    40741 ttaagcaact cggcaatttt tagcatcaca gcttcgccac ctggggcgca acggttgatt
    40801 ttttcaccgt tacagctgat ggcttccgcg taggggcgac agccgggata accgcactga
    40861 ccacactggc tctgcggtaa gatttcgtca attttctcaa cgaccggatc gtcttccacc
    40921 gcaaaacggc gggaggcata acccagaatg gcgccaaacg ccaggcccag caggctcacg
    40981 gcggcaacgg caatccagat agcattcatt acaacttcac caaaccacta aagcccataa
    41041 aggccagaga cataagacct gcggtaatta acgcaatggc attaccgcga aaaggtgccg
    41101 ggacatcagc cacagcaagg cgttcgcgga tggtggcgaa gagcaccatc accagcgaga
    41161 aaccgacagc ggcggaaaaa ccgtacagcg ccgactgcaa gaaattgtgc ccgagattga
    41221 tattcagcaa cgccacgccg agcactgcac agttggtggt gataagcggc aaaaaaatcc
    41281 ccagcagacg gtaaagcacc gggctggttt tgcgcaccac catctcggtg aactgcacga
    41341 ccacagcaat caccagaata aatgccaggg tgcgtaggta aatcagatta agtgggatca
    41401 aaatccacgt atcgataagc caggcgcaaa tagacgtcag cgtcatcaca aacgttgttg
    41461 ccagccccat gcccatcgcg gtttccagtt ttttggaaac ccccataaac ggacagagac
    41521 cgagaaactt gaccagtaca aagttattga ccagtacagt tccgacaaac agtaacaggt
    41581 agtcagtcat ttttcggcct gaaataaaaa agccgcctat tatccgttaa tccagagcag
    41641 gcgacaacag gttaactgta aggttattac ggattcacaa acgtcgcttt tacccgcgac
    41701 gagcgtttga aataggggac gatcaatgcg gttgccaaca aagtaaacag caactggcga
    41761 acggctatgc caacttccac gggtgaaaaa gcgaatgctt ttactgccag caatacggaa
    41821 ataagcagcc agataatata gtgtttagga acacaacgcc gacgtttaaa gaaagcgatg
    41881 gtcaaccaca gtgtgtaata ccacatggcg atagcggtaa taaaagaaac gccccataag
    41941 atttgtgtcg tcagtgcttg tccgccgagc gtttgaaatg tttgcggaga agataacgca
    42001 gcggtataca gcaacaacgc cagcgtcgta ctcaacagag cgactaaaag ccaggccaac
    42061 gggccaagca accagcctcc aatacgttgt ggcgttgtgg tggtcatagg ttctccagaa
    42121 tagcgacgca aacaggcaaa tctaatttta ggggcgttat tataaggctt ttataaccga
    42181 tcgctacttt ctttattgga catagtgcca gacggacttc ggtacctggc cgaggtcaaa
    42241 caaacgcccg ccagaaacca gctctgcgcg acggtgatcg gcagcacgat acatattgat
    42301 cagttcctga tcgtccgtca gggtgtaatt aagatggtcg tagagttttt ccagactttc
    42361 gagtgaactg attttgcgaa attttaataa gtagtcctga acagtcataa aaggtccata
    42421 aaataattaa taccaaaggg gtaggtatta ttagtgatga taaaatttca ccaaaagtga
    42481 acgcttgtgt tattacaata ataagaatgc gctgatggca aatattcagg gatgaaaaac
    42541 tgctctgaaa agagtaacgc gctttactat ccctgacaat caccaacaac atcgaacaag
    42601 ataataaatt cctggtttaa tatccgacaa gtgaaaacat gcacccggac gggcagcatg
    42661 tcgctccaca agtgcagagc ttacttgtgt tgtaccgaag cactctgttc aggtggctga
    42721 tagttgtcaa tgtgactcgc cacgccaaga agaatgactg aaacgacaag aacgatccaa
    42781 cctgttaatt caataagacg attcattaca gcccacatct ctcttgattg atccattaac
    42841 ttcagggggt aaatgttact tagcaataat agctccagca agatttttac tgaggttttt
    42901 gcgatattta gcttttgtcg ttggaaaatt cgctattttt ttgacttaag ttaaacaaca
    42961 tcccttattg ctggcaggtt attaaactgt tgagcgtggg taaaggatag tgtcaaatag
    43021 ccatcatact tcaatgagag gcaatgacat gagcgacaac atccgtgttg ggttgattgg
    43081 gtatggttat gcgagcaaaa ccttccatgc gcccctgatt gcgggcacgc ccgggctgga
    43141 actggcggta atctccagca gtgatgaaac aaaagtaaaa gccgactggc caacggttac
    43201 ggttgtctct gagccgaagc atctgtttaa cgatcccaac atagacctga ttgtcattcc
    43261 tacacccaac gatacccatt tcccgttagc caaagcggcg cttgaggcgg gtaaacatgt
    43321 ggtcgttgat aaacccttta ccgtgacact gtcacaagcg cgagagctgg atgcgctggc
    43381 aaaaagcctg gggcgtgtgc tgtctgtatt ccataaccgt cgttgggata gcgatttctt
    43441 gacgctaaaa ggtttgctcg cggaaggcgt gctgggtgaa gttgcttact ttgagtctca
    43501 ttttgaccgc ttccgtccgc aggtgcgcga tcgttggcgt gaacagggcg gcccaggcag
    43561 cggtatctgg tacgatttag caccgcatct tcttgatcag gccattacgc tatttggttt
    43621 accggtcagc atgacggtag atttggcaca gttacggccc ggagcgcagt cgaccgatta
    43681 tttccacgcc atcttgtcct atccacagcg gcgagtcatt ttacacggta ccatgctggc
    43741 agccgctgag tcagcacggt atatcgtgca tggatcccga ggcagttatg tgaaatatgg
    43801 cctcgatcca caggaagaac gtctgaaaaa tggcgagcgt ctgccgcagg aagactgggg
    43861 ctacgatatg cgtgatggcg tacttacccg cgtggaaggt gaggaacgtg tcgaagaaac
    43921 gctgttgacg gtgcctggga attatccggc ttactatgcg gcgattcgtg atgcgttaaa
    43981 tggcgatggt gaaaatccgg ttccggcaag ccaggcaatc caggtaatgg agttgattga
    44041 gctgggcatc gaatccgcca aacatcgcgc gacgttgtgc cttgcgtgaa aaatggatgg
    44101 tgcaatttgc accatccatt tttgttactt cgcggcgact ttttctcgca gtgcgcgttt
    44161 ttcctctgcg ctgaggaaag ccatttccag accattaatc tgtgcctggc ggatttgctc
    44221 acgggataac ccagcagctg gcgcggcaac ggtatattcg tgaatgatat ccactccctg
    44281 tacgccggga tcgtcagtgt taatgctggc acgaatgcca tgctcaagga acgttttcag
    44341 cggatgtgca gccagctctg ctacggtgct ggtctgaata ttggaggtca gacaggattc
    44401 aataccaatt tgttgctcgg cgagaaaatc catcagcgcc cgatcttcaa tggcttttac
    44461 gccatgtcca atacgctccg cacccagttc acgaatcgcc tgccagatgc tttccggccc
    44521 ggcagcttcg cctgcatgga cggtaatatg ccagcccgca tcacgcgcgc ggttgaagtg
    44581 agaaaggaac agacttcccg ggaatccaag ttcatcacca gctaaatcaa gtgcggtaat
    44641 ctggtcacgg tgggctaaaa aggcctccag ctcttgctga caggcggctt cgccgaaggt
    44701 tcggctcata atgccgataa gcttcgcctg cacaccaaag gtgcggcaac cttcacgtac
    44761 gccatcgatc accgcttcga caacacccgc tacaggcagc ttatgtgcca ttgccatgta
    44821 gcctggtgaa aaacgcagct cgacatagcg caggccgtta cgggctgcat cttcaatgtt
    44881 ttcaaatgcc acgcggcggc aggcatcaag agaggcgaga actttaacgc cccagtcaag
    44941 tttggtcaga aagctcacca gatcgggttc gttggcaatg acctgaacgt ggggaatcag
    45001 tgtttcaagg gattgtgcag gaagcgagat attatactgg cggccaagtt caagaatggt
    45061 ctggggacga atgttgccat caaggtggcg atggatatca gttaatggca gggtggtatc
    45121 aatcatggtc gcactctttt tctggatata aaagtgcgca ccattataaa aacaaagcag
    45181 ggtaaaaatc cacgattgcg caacgatttt tacgttgcgc aattggggtt agcgaacagc
    45241 gcggatggcg tttattaatc cagccacacc tttttccagt ttcgaacgtg ggcagccggc
    45301 attgagacgg acaaaaccac gaccttcttc accataggta taccccggca tgatcgcgac
    45361 tttttcttgc tcgataagcg ctttttgcaa cgcgttgtcg tcaatattca acggacgtag
    45421 atcaagccag gccagataag tggattgcgg gatctgccag ttgagttcag gaaacgcggc
    45481 gttcatttta tctgagatat acgtcaggtt atctttcaga tagacgcgta aggcatccag
    45541 ccacggtgca ccttgctgat aggcggcgat atgggcagtt aacgccagta ccgaagggga
    45601 agaaagccca tcacgtcctt tcagtgccga taaataggca tcacggctgc tgctattttc
    45661 tataatcccg taagcaccgg tcagcgcggg aatattgaaa cttttcgatc ccgacgtcag
    45721 caacgcccag tctccccgcg ccacattact ccagggaata tgcggctgct cgccccagac
    45781 catatccata tggatttcat cggaaataac ccgcacatca tgacgctcgc acaggtcagc
    45841 catgatctcc agctcatcgc acgtccacac tttcccggta ggattctgtg ggttacacag
    45901 gagcataatt ttacattctg gtttcgccaa aacggcttcc agcttgccca tatcgcaaaa
    45961 ccagccgtca gcctgcttct ctaaagcaac gggcattact gtgcgctggt taccttcaat
    46021 ggccttgtaa aatgcgtcat aggcgggcgt gtggatcacc acgccttcac ctgtttcaga
    46081 ccactgacga atcagttctg aaaccatata gatgacagaa gggccataca ccaccgtctg
    46141 cgtatcaatt gcggtgtaat gctgggtgga aaaccagtgg gcaatagccg cgagaaactc
    46201 atcgtttttc cagcggctgt agccaaatac gccgtgcatc aggcgctgat tcagcgcctc
    46261 gataatgcag ggggcagtgg caaaatccat gtctgaaatc gtgaacggta acaggtcagc
    46321 agtgccgaaa cggtcagcga cataatccca ctgtgtacac catgtgccat gacgatccac
    46381 gacctttgaa aaatcgaaca tatcttatcc ttatgcctgg acagtatgca tcagaccggc
    46441 catttcatct tttactgact gaacttgtgg cccgataaca acctgcaggt tatgttgatt
    46501 aagttgtact acgccaattg cccgattgtc cttcagtgct tgcacattaa caagcgacat
    46561 atcttccaca gacaaacgca gacgggtaat gcagttatcg aggctgacaa tattgtcggc
    46621 accgcctaat gcttcgagga ttgcaggaac gttgtaacct gatttacccg gcgcaccggc
    46681 aacggctttt tcgattgagc tggcaacttc gctatcgcgc cccggggttt tcagattgaa
    46741 gcgggtgata gcgaaacgga agatgacgta gtaaacgaca aaccagattg ccgccaccac
    46801 tggcaccatg taccacttgg ttgacagacc atgcaaaata ccgaacacca cgaagtcgat
    46861 gatattgccg tcggtattac cgatggtgac gccgagcaca gacatgacgg tgaagccgag
    46921 gccggttaac agcgcgtgga tgacatacag aactggcgct acgaacagga acaggaattc
    46981 cagcggttcg gtagtgccgc caacgacgca ggcgatcagg ccagaaatca gcagaccttt
    47041 aattttatgg cgattttccg ggcgcgcgca gtgatacata gctaacgctg cacctggcag
    47101 accgccgaga aacgcaggca ttttaccttg cgaaaggaaa cgcgtgacgc tttcagaaaa
    47161 accgtgagtg gtcgggcaac tcaattgcgc ctggaagatg gtcagtgcgc cgctgacggt
    47221 ttgaccgcag acctcctgcg tgccgcctgc gtcggtaaag cgaattaatg ccaccagaat
    47281 gtgatgcaga ccaaacggta acagcagacg ttcaccggta ccaaacagca tcggtccgaa
    47341 atcacccgcg ctgtttatca tatggcccaa gccgctaata cccatggcga aaatcggcca
    47401 gactaatgga atcaccaggc cgacaaggcc catcaccagc gaggagataa ttggtacgaa
    47461 gcgcgtaccg ccgaagaatg ccagcgcatc cggcaggcgg atattatgga aacgctcatg
    47521 cagcatccag acgataatac cggcgatcac cgcaccgagg atcccggtat cgatcgactg
    47581 gatcccaaga atgctctgga tgttattggc tttcagaacg gcggcatctg tggttggcag
    47641 aatgccttta ttggtcaacc agaagtttac cgcgaggttc attaccgcat aaccgacgaa
    47701 gccagcgaat gccgctacgc ctttattttc gcgtgccagg cccagcggga tggcgataca
    47761 gaacatgaca ggcaggaaac taaaagcaaa cgagccaatc ttactcatcc aggtaaagat
    47821 agcctgcaac acggggttgc ccaggaccgg gatcagggtt atgacatcat ggctgctaag
    47881 agaactacca atgccgagca taatgccgca gaacgacaat aatgccacgg gtaacatgaa
    47941 ggttttgcct aactgctgga agaactccca cagcgtgact ttcggtgctg ttttcgccgt
    48001 cataaaacga ctcctcgtag agaacaatct ggattcagta aattgcgcga gaagataaaa
    48061 cgttttatca aattttagtg agtcataaat cacattacgc aacgataata gcgggtataa
    48121 gataaataaa aggtaaaacg ttttatctgt cacataatca gggagtaggt catctgcatg
    48181 gctaccgcca aaaaaataac cattcatgat gttgcgctgg ctgcgggcgt gtcggtaagt
    48241 accgtttcgc tggtgcttag tggcaaaggg cgaatctcta ccgccacagg agaacgcgtt
    48301 aacgccgcca ttgaagagct gggatttgtg cgcaatcgcc aggcgtcggc gctgcgcggc
    48361 gggcaaagcg gcgtcattgg tttgatcgtc cgtgatttat ctgcgccgtt ttacgccgaa
    48421 ttgacggccg gattgacgga agctctggaa gcgcagggac ggatggtttt tttgcttcac
    48481 ggcggtaaag acggcgagca gctggcacag cggttttcac tgttactgaa tcagggtgtc
    48541 gatggtgtgg taattgccgg ggctgcagga agcagcgatg acctgcggcg gatggcagaa
    48601 gaaaaagcta tcccggtgat tttcgcttcc cgtgccagtt atcttgatga tgttgatacg
    48661 gttcgcccgg acaacatgca ggctgcacag ttgttgacgg agcatctcat tcgcaatggg
    48721 catcagcgga tcgcctggct gggagggcaa agttcctcat taacccgtgc agaacgggtg
    48781 gggggctatt gtgcaactct actaaaattt ggcctgccgt ttcacagcga ttgggtgttg
    48841 gagtgcactt ccagccagaa gcaagccgcg gaagctatca cggcgctttt acgtcataac
    48901 ccgaccatca gtgccgtggt ttgctataac gaaactattg cgatgggggc atggtttggt
    48961 ttgctgaaag cagggcggca aagcggggaa agcggagtcg atcgttactt tgagcaacag
    49021 gtttctctgg cggcatttac cgatgcgaca ccaaccacac ttgatgatat acctgttacc
    49081 tgggccagca cgccagcgcg ggaacttggt atcacacttg cggatcgcat gatgcaaaaa
    49141 atcacccatg aagagacgca ttcacgcaat cttattattc ccgcccggct cattgcggcg
    49201 aaataatcct ctctttatct gctatacttg gtagtgtcct ttcctcaagg ttaatgataa
    49261 aaaatgtggc acacaggcgg tgccttttat ttttactgaa gttcacagga ggtttatgtg
    49321 tttaattctg acaacctgag actcgacgga aaatgcgcca tcatcacagg tgcgggtgca
    49381 ggtattggta aagaaatcgc cattacattc gcgacagctg gcgcatctgt ggtggtcagt
    49441 gatattaacg ccgacgcagc taaccatgtt gtagacgaaa ttcaacaact gggtggtcag
    49501 gcattttcct ggcgttgtga tattacttcc gaacaggaac tctctgcact ggcagacttt
    49561 gccgtcagta aactgggtaa agttgatatc ctggttaaca acgtcggtgg cggtggtcct
    49621 aaaccgtttg atatgccaat ggcagatttt cgccgtgctt atgaactgaa tgtgttttct
    49681 tttttccatc tgtcacaact tgttgcgcca gaaatggaaa aaaatggcgg tggcgttatt
    49741 ctgaccatca cttctatggc ggcagaaaat aaaaatataa acatgacttc ctatgcatca
    49801 tctaaagctg cggccagtca tctggtcaga aatatggcat ttgacctggg tgaaaaaaat
    49861 attcgggtga atggcattgc gccgggggca atattaaccg atgccctgaa atccgttatt
    49921 acacca
//
LOCUS       DAJTYF010000017        44110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTYF010000017 DAJTYF010000000
VERSION     DAJTYF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.017"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(1307..2989)
                     /gene="glcA"
                     /locus_tag="OO590_001093"
     CDS             complement(1307..2989)
                     /gene="glcA"
                     /locus_tag="OO590_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HCR6636545.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFAGVIGVITLLQAYVFTGMLVS"
     gene            complement(3344..5515)
                     /gene="glcB"
                     /locus_tag="OO590_001094"
     CDS             complement(3344..5515)
                     /gene="glcB"
                     /locus_tag="OO590_001094"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR6636546.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGIVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5537..5941)
                     /locus_tag="OO590_001095"
     CDS             complement(5537..5941)
                     /locus_tag="OO590_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HCR6636547.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(5946..7169)
                     /gene="glcF"
                     /locus_tag="OO590_001096"
     CDS             complement(5946..7169)
                     /gene="glcF"
                     /locus_tag="OO590_001096"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HCR6636548.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(7180..8232)
                     /gene="glcE"
                     /locus_tag="OO590_001097"
     CDS             complement(7180..8232)
                     /gene="glcE"
                     /locus_tag="OO590_001097"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR6636549.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFCYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8232..9731)
                     /gene="glcD"
                     /locus_tag="OO590_001098"
     CDS             complement(8232..9731)
                     /gene="glcD"
                     /locus_tag="OO590_001098"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HCR6636550.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANELGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9977..10747
                     /gene="glcC"
                     /locus_tag="OO590_001099"
     CDS             9977..10747
                     /gene="glcC"
                     /locus_tag="OO590_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR6636551.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10871..12013)
                     /locus_tag="OO590_001100"
                     /pseudo
     CDS             complement(10871..12013)
                     /locus_tag="OO590_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12417..14105
                     /locus_tag="OO590_001101"
                     /pseudo
     CDS             12417..14105
                     /locus_tag="OO590_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            14102..15016
                     /locus_tag="OO590_001102"
     CDS             14102..15016
                     /locus_tag="OO590_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6636552.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            15049..15297
                     /locus_tag="OO590_001103"
     CDS             15049..15297
                     /locus_tag="OO590_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6636553.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            15297..16469
                     /locus_tag="OO590_001104"
     CDS             15297..16469
                     /locus_tag="OO590_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6636554.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(16505..17584)
                     /gene="lptG"
                     /locus_tag="OO590_001105"
     CDS             complement(16505..17584)
                     /gene="lptG"
                     /locus_tag="OO590_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6636555.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17581..18651)
                     /gene="lptF"
                     /locus_tag="OO590_001106"
     CDS             complement(17581..18651)
                     /gene="lptF"
                     /locus_tag="OO590_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6636556.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPWQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18682..19242)
                     /locus_tag="OO590_001107"
     CDS             complement(18682..19242)
                     /locus_tag="OO590_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6636557.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLSNE"
     gene            complement(19254..20078)
                     /locus_tag="OO590_001108"
     CDS             complement(19254..20078)
                     /locus_tag="OO590_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636558.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20078..21427)
                     /locus_tag="OO590_001109"
     CDS             complement(20078..21427)
                     /locus_tag="OO590_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6636559.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21473..22231)
                     /locus_tag="OO590_001110"
     CDS             complement(21473..22231)
                     /locus_tag="OO590_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636560.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22263..22976)
                     /locus_tag="OO590_001111"
     CDS             complement(22263..22976)
                     /locus_tag="OO590_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6636561.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23150..23842
                     /locus_tag="OO590_001112"
     CDS             23150..23842
                     /locus_tag="OO590_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6636562.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23891..25390)
                     /gene="pitB"
                     /locus_tag="OO590_001113"
     CDS             complement(23891..25390)
                     /gene="pitB"
                     /locus_tag="OO590_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6636563.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25682..27541)
                     /gene="gss"
                     /locus_tag="OO590_001114"
     CDS             complement(25682..27541)
                     /gene="gss"
                     /locus_tag="OO590_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6636564.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27746..28612
                     /gene="yghU"
                     /locus_tag="OO590_001115"
     CDS             27746..28612
                     /gene="yghU"
                     /locus_tag="OO590_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6636565.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28735..28983)
                     /gene="hybG"
                     /locus_tag="OO590_001116"
     CDS             complement(28735..28983)
                     /gene="hybG"
                     /locus_tag="OO590_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6636566.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29117..29458)
                     /gene="hypA"
                     /locus_tag="OO590_001117"
     CDS             complement(29117..29458)
                     /gene="hypA"
                     /locus_tag="OO590_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6636567.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29451..29939)
                     /gene="hybE"
                     /locus_tag="OO590_001118"
     CDS             complement(29451..29939)
                     /gene="hybE"
                     /locus_tag="OO590_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6636568.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29932..30426)
                     /locus_tag="OO590_001119"
     CDS             complement(29932..30426)
                     /locus_tag="OO590_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6636569.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLSDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30426..32129)
                     /gene="hybC"
                     /locus_tag="OO590_001120"
     CDS             complement(30426..32129)
                     /gene="hybC"
                     /locus_tag="OO590_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6636570.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32126..33304)
                     /gene="hybB"
                     /locus_tag="OO590_001121"
     CDS             complement(32126..33304)
                     /gene="hybB"
                     /locus_tag="OO590_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6636571.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33294..34280)
                     /gene="hybA"
                     /locus_tag="OO590_001122"
     CDS             complement(33294..34280)
                     /gene="hybA"
                     /locus_tag="OO590_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6636572.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34283..35401)
                     /gene="hybO"
                     /locus_tag="OO590_001123"
     CDS             complement(34283..35401)
                     /gene="hybO"
                     /locus_tag="OO590_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6636573.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35590..35877)
                     /locus_tag="OO590_001124"
     CDS             complement(35590..35877)
                     /locus_tag="OO590_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6636574.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35996..36883)
                     /locus_tag="OO590_001125"
     CDS             complement(35996..36883)
                     /locus_tag="OO590_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6636575.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            37040..38080
                     /gene="gpr"
                     /locus_tag="OO590_001126"
     CDS             37040..38080
                     /gene="gpr"
                     /locus_tag="OO590_001126"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6636576.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38120..38614)
                     /locus_tag="OO590_001127"
     CDS             complement(38120..38614)
                     /locus_tag="OO590_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6636577.1"
                     /translation="MERFFENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38805..39690
                     /gene="yghA"
                     /locus_tag="OO590_001128"
                     /pseudo
     CDS             38805..39690
                     /gene="yghA"
                     /locus_tag="OO590_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
     gene            complement(39962..40387)
                     /gene="exbD"
                     /locus_tag="OO590_001129"
     CDS             complement(39962..40387)
                     /gene="exbD"
                     /locus_tag="OO590_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6636578.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40394..41128)
                     /gene="exbB"
                     /locus_tag="OO590_001130"
     CDS             complement(40394..41128)
                     /gene="exbB"
                     /locus_tag="OO590_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6636579.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41380..42567
                     /gene="metC"
                     /locus_tag="OO590_001131"
     CDS             41380..42567
                     /gene="metC"
                     /locus_tag="OO590_001131"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6636580.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42707..43366
                     /locus_tag="OO590_001132"
     CDS             42707..43366
                     /locus_tag="OO590_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6636581.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43406..>44110)
                     /gene="yqhC"
                     /locus_tag="OO590_001133"
     CDS             complement(43406..>44110)
                     /gene="yqhC"
                     /locus_tag="OO590_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6636582.1"
                     /translation="RVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQ
                     ELLMDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREI
                     LYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFH
                     HNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFG
                     VTPGEDAARMRAMQGN"
BASE COUNT        10942 a        11762 c        10838 g        10568 t
ORIGIN      
        1 tacgtaccgt ttaaacaaag acaatacgtc tctttatttc tcataaaacc ccaatccctt
       61 acagggctta ttgaaaaatt taaaaatcac aaatttctca atactcgaaa tgagatcaca
      121 taagaaatca aaaagccttc tcatctattt taagttttcc caacggaaaa taagagcttt
      181 cattaatact gaaccacttt actgttctat ttgttcaggt attgcattac ggtgtgctga
      241 gatccatcgg tgcatccctg tatcccgcga tgagactaaa atagtcgtga tgtaagctcg
      301 tatcagattc ttagtgaata ccacatttgt tataattaag tgaaaagata cattgctgat
      361 ttacattatt tggtttacaa aatgtcatca cttgcagagt tatcgctgac agataccttt
      421 aatctcttac tactttcgtg ttattgcaat acaaccatcc gtaaagcagg gctattttta
      481 gcaggaaata ctcaggtgtc aacattcgaa ctccaactct gaaatattca tataaattaa
      541 tgattttaat gattttttca tttcttcgcg cctaataaaa gtattcaaat tcagatgatt
      601 tttctgctca acagacacaa acaggcaata atactctcca aagcgaacaa tatttggcat
      661 tacgatttaa tgcttcataa tcaccaaatt atttaaccat aaattttctt atttctaaca
      721 aaatgattgg tgtgttgttg gagttaaaat acatacataa tattaacatc tgccaacatt
      781 tcactttccg caagacggat gatttcgaat cctcatccac gttaccctat cccaaatatc
      841 tttattaata ggcagtaacg aacactgtta tagcaagatt aacttattaa cctaacggca
      901 tcttttatgt aacaagtgga gataaattgc ttaaaattga tcctcgacaa atcttaaaag
      961 ttctcattat tctgcaaaat cagctttgcg aataatctta tgtttttggg agttttggga
     1021 attttgatct actcattgtt ctagtgaaaa atataaataa gagaaaaaac gccattcgtc
     1081 atgaaagcaa cagatgaagc aatgtaataa catactaaaa ttgatctcat gaaagtgggt
     1141 actttataac cagcacagaa gccaagttgt ataaatatta aacaattcgc atcgtgaaac
     1201 ggagactacg tataaagaag ccaatcgtaa aaacggttcc ctggcgagat aaacggcaga
     1261 caaatggaaa gccgggtgat atttaccttc atccggcttt attgatttac gagactaaca
     1321 tcccggtaaa cacatacgcc tgcagcaggg tgataacgcc gataacgccg gcaaaaatca
     1381 gactgtgctt cacggtgtag cggaacagtt cagattctcg gcccaccatg cccgtcgcgg
     1441 cgcaggccac ggcgatagat tgcggggaga tcatcttgcc agttacgccg ccgctggtat
     1501 ttgctgccac cagcagagtg tcagagacgt tgatttgctg cgccgtggtc gattgcagtg
     1561 aaccaaacag ggcgtttgaa gaagtgtccg acccggtaag gaatacgccc agccagccga
     1621 gaaacggtga gaaaaacggg aacatcacgc ctgtacctgc cagtaccagc gccagcgtgg
     1681 tggacatgcc ggaatagttg gtgacgaagg cgaatgccag caccatgcca atcgacaata
     1741 tcggccactt cagactaatt agcgtttcgg caaagacgcc aatccctttc ttgatgccca
     1801 cgccgaggat gaagatagag ataatcgccg caataaaaat agcggtgccg ccagccgaga
     1861 gaggttcgaa tttaaacacc gcatccattg gcgttggttg ggcgacaatg ggtgccgctt
     1921 tcaacacttg ttgatgcaaa tgagggatct ggaaattaat caccagtgaa taaaacgcgc
     1981 cgcccggagc aaataacgct ttaaacggct tcatggtcca gatggtgacc agcaccgtta
     2041 agattaaaaa cggtgaccac gcacgaatga tttgccccag actatattct gaaggcacgg
     2101 gaccgccaga agatggctta tttaccacca tcgcacctgc ggattgcccc atgctgattg
     2161 ccgtttcggt atttttcggc cgccagactt taaggaataa agcgagtgag acgatactca
     2221 ccagcgccga agtaatatcc ggcagttccg gaccaatata gttagaggta aagaactgag
     2281 tgacagcgaa gcttccccca gcaaccagcg ccgctggcca cgtctctttc acccctttcc
     2341 agccgtccat cattgctacc agccagaacg gcacaagaac cgacaggaac ggtaactgac
     2401 gtcccgccat tgcgccaatg tggaacggat cgattcccgt tacctgaccg gcgaccagaa
     2461 tcggcacgcc caacgcacca aacgccaccg gcgcagtatt ggcaatcaga cacagccccg
     2521 ccgcgtataa cggtttgaag cccaggccca ccagcagcgc accggtaatc gccaccggcg
     2581 caccaaagcc agccgctcct tccagcaacg caccaaagga gaaaccaatc agtaacacct
     2641 gcaaacgctg atcgtcggtg atggagataa ccgagctgcg gataatatcg aactgcccgc
     2701 tggcaacggt taatttatac aggaacaccg ccgcgacaat aatccacgca attggccata
     2761 atccataaat aaagccatag cccgcagcag caaatgccat atcaatcggc attttaaagg
     2821 cgaatatcgc aatcaggata gataatataa gggttattgc tccagcgaca tgtcctttca
     2881 gacgtaatac cgcgagtgca acgaagaaga atattatcgg gatcagggcg accagagcgg
     2941 atagccccag tcctcccatc ggcatataca tttgggtcca ggtaaccata ttgttcagtc
     3001 tcttattatc tttatatgct tgatatactt aaggttgtaa taagcaaaag aggactgaac
     3061 tgtaaaatat aggcgttata ctttacagca acagtacgcc gctaacgcaa ttgctacctc
     3121 tggcataaca agtatatcgg gtaagggttt ctgttccgca cacgcaggcg cagagtatcg
     3181 ttaagatgtc catattgttg ttttaggccc gctagtaatg cgctacgggt atttaatatt
     3241 gttaaaccct gataatcgct ccggttattt ccgggataaa tgtactaccg cagttactat
     3301 catagccccg acaataaaac ttgccggggc ttttttgacg ctattaatga ctttcttttt
     3361 cgcgtaaacg ccaggcgtgt aataacggtt cggtataacc gtttggctgt ttcacgccga
     3421 ggaagattaa atcgctggca gctttaaaag cacacgagtt agcgaaattc cccgccatcg
     3481 gacgataagc cggatcgcca gcattttgct gatcaaccac tttcgccata ttctccagcg
     3541 acgcctgcac ctgttctttg gtcagaatac cgtgacgtaa ccagttggcg atatgctggc
     3601 tggagatacg cagcgttgcg cggtcttcca tcagcgccac gttgtgaata tccggcactt
     3661 ttgaacaacc aatcccctgc tccacccaac gcaccacgta ccccagaatc ccctgcacgt
     3721 tgttatccag ctcttgttgg atctcttgcg ccgaccagtt agcgttttca gccaccggaa
     3781 tagtcagcag atcgtccagc agcggttcaa attcagcatt gaactcggtc tgggcaatgt
     3841 tggcttgcac gctctgtacg ttggtttggt ggtagtgcag cgcatggagc gtagcagcgg
     3901 ttggtgacgg aacccaggct gtgtttgccc cggcacgcag ttggtcgccc ttctggctgt
     3961 acatgtctgc catcaggtcc ggcattgccc acatgccttt accgatttgc gctttaccgc
     4021 gcagcccaca gaacagaccg gaaagcacgt tattacgttc gtaggctttg atccacggcg
     4081 tcgatttcat ctggttttta cgcagcatcg ggccagcttc catcaccgaa tgcatttcat
     4141 cgccggtacg gtcgaggaaa ccggtattga tgaacgccac gcggttgcgc gcctgagcga
     4201 tacagctccg caagttcagc gaggtccggc gttcttcatc cataatgccc attttcaggg
     4261 tattcggtac tataccgagc attgtctcaa tgcgggtaaa cagtttgttg gcgaacgcca
     4321 cttcctgcgg accgtgcatt ttcggtttca caatatagac gctgccagtg cgcgagtttt
     4381 tctgcacttt taaatcatag agggcaatcg cgccagtcat gacgccatca agaatacctt
     4441 ccgggatttc attgccttcg ctgtcccaaa tcacaggaat ggtcatcaaa tgacccacgt
     4501 tgcggataaa cagcaacgag cgtccgtgca gagaaatttc agagccatcg gcggcggtgt
     4561 actgacgatc gtcattcagt ttacgtacga tttgccgacc gtttttctcc attttctctt
     4621 gcagagtccc ctgcatcagg cccagcagat tgcggtacag caggatttta tcttccgcat
     4681 caaccgccgc gaccgaatct tcgcagtcga gaatggtact gatagcagct tcgacgataa
     4741 catcgttgat gtgcgccgga tcgtctttgc caatccgccc gttggcatca atttgcagtt
     4801 caatatgcag gccgttattt ttcagcaaaa tgcaggtcgg cgcagcggca tcgccacggt
     4861 aaccgacaaa ctgtgctgga gtacgtaacg tggtttcttt accatttttc aactggatgc
     4921 gtaattgttt atcgaccacc ttaaacgcca ccacatcctg atagctgccg ttttccagcg
     4981 gtagtgattc atcgaggaaa cgtcgaaccc aggcgataac ctgctcaccg cgctgcggat
     5041 cgtagccact gaccatcgcc ccttcctgcg ggatgatgtc gctgccgtat aacgcatcgt
     5101 acagtgagcc ccagcgagcg ttcgccgcgt tcagcgcgta gcgggcgttc attgccggaa
     5161 ccaccagctg tggccccgcc tgactggtga tttcgctgtc aatgcccgtg gtttccaccg
     5221 tcacgcgctc cggttgcggc accaggtagc ccagttcacg aaggaaagat ttataggccg
     5281 ctttatcttt taccggcccc ggattgctgc gatgccactc atcaagcgcc gcctgaatgc
     5341 gatcgcgttc tgccagcaac tgacgatttt ctggtgccag atcgtgaacg atctcatcaa
     5401 cattgtgcca gaatgcagca gcgtccagtt ccacgcccgg caaaacttct ccatccacaa
     5461 aacgtttaaa attggcgtca atgcgtaaac ggccctgggt tatggtttga ctcattgttt
     5521 ctctcctcgt tttcgcttat ttcgccaaca ccgctgctgc cgctttcgcg acctgcgcat
     5581 cctgtgctcc ggttaaacca gaaacgccca cggcaccaat aatttgccca tcaacaacaa
     5641 ccggtacgcc gccttccagc gacgtcagta acggcgcagt cacgaacgcg gtacgtccgt
     5701 tgttcaccat ctcttcatag cccttagttt cacgacgccc cagcgcggcg gtacgcgctt
     5761 tctcctggga gatgtaagcc gcaatcggcg cgcaatcgtc catgcgactt aacgccagca
     5821 gatgaccgcc gtcatcggca acagcaatgg aaacagacca gttatttttc tgcgcctctt
     5881 cctgacctgc ggcaataatt gcactcgcca tttgctggct aagaatgact ttagttttca
     5941 tttttttatt ccttttcaag ggcttgttct acaatttcaa tccagtgacg cacagaggta
     6001 cgaccggcgc tcgccagatg cgtctggcaa ccaatgttgg cggtgacgat catttccggt
     6061 ttgccgcttt ccagcgcatt cattttgtta tcccgcagct ggcgtgccag atcgggatgc
     6121 gttaacgcat atgttcccgc tgaaccgcag cacagatggc tgtcgggaac gtccgttaag
     6181 gtaaatccaa gacgaagtaa cacgttttcc acttcgccgt tcagcttttg cgcatgttgt
     6241 agggtacacg ggcagtggaa ggccagtttt ttatcgccgc gaattgccag tttttccagc
     6301 ggttcctcgc gcagaagttc gactaaatcg accgccagtt cactgacctg acgcgcttta
     6361 tcggcatata acgcatcgtt tttcagcatc tgcccatact ctttgacgaa cgcgccgcaa
     6421 ccgctggcgg tttgcaaaat tgcctcggca cctgcttcaa tcgcgggcca ccaggcatca
     6481 atattattgc gcgcccgtgc caaccctttc tcctgcgcat taagatgata gtccaccgcg
     6541 ccacaacagc ctgcttcgtt agctgacatg acgctgatcc ccagacgatc cagcactcgc
     6601 gcagttgccg cgttggtgtt gggcgaaagc gtaggctggg cgcagccttc cagcattaaa
     6661 acccgacgct tatggcgcag cggcggacgc ggtttagctt tcaccgtttc agcaggcagt
     6721 tttgctctga cctgttccgg taaaaacggt cgcagcacca gccctacctg cgtcagcgca
     6781 cggaagaccg ccggacgcgg cactacctgg cgcaatcctt cgcgcagcat tcgctccggc
     6841 agtgggcgtt tcactttctg ctcgacaata tcacgcccga tatccagcaa attgtgatag
     6901 cgcacaccag aaggacaggt ggtttcacaa ttgcggcaag tgaggcagcg atcgagatgc
     6961 tcctgtgttt taagcgtgac ttcgttgcct tccagcacct gtttaatcag atagatgcgc
     7021 ccgcgcggcc cgtccagttc atcgcccaga agctgatagg ttgggcaggt tgcggtacaa
     7081 aatccgcagt ggacacaggc gcgcaggatg ctgtcggctt ccagcgcgcg cgcgttctgc
     7141 cgcatctctt cggttaatcg ggtttgcata gcctgctcct caaagttccg cgtacatgcg
     7201 accggggtta aacacgccgc aagggtcgag ctgctgttta agctgctggt gatagcagaa
     7261 taaaggagcc gatagcgggg caaagccacc atctccggca ctaaagcggg tcgcatgacc
     7321 gccagcgttg cgggcgatgc gatggatttg attgtcatcg gctgtcgatt tcagccagcg
     7381 taacgccccg ccccagtcga tcagttgctc gccgggtaaa tccatcatcg gcgcatcgct
     7441 gggtaatgaa atgcgccata aggtacctgg taacgagaag aacggcagtt gttgttcacg
     7501 caattgctgc cagaactgac ccgcaacctc ttcgccaccc agcagttcac gcgctgcttt
     7561 taccgatcct tcgccgccct caaggcggat ccacaacgca ttgtcgaagt aacataagcc
     7621 actaatgggt aatggctgga gttgccactc ggcgatttca ctcatggctt cttgcaggct
     7681 gatttcccga cgcaggctca gagaggcacg cggtcgcggt aacactttca ttgagatttc
     7741 agtgagcacg ccaagacaac cgtagcttcc ggccattaac cgtgagagat cgtatccggc
     7801 aacgtttttc atcacttcgc caccaaaacg cagatgtttt ccagtgccgg taatgatgcg
     7861 cgtgccgagg acaaaatcgc ggaccgaacc gctccacggg cgacgcggcc ccgccagccc
     7921 gcaggcgacc atcccgcccc aggtggcttc ttcaccataa tgcggcggct cacagggaag
     7981 catttgcccg gcgctctcca gcgccgcttc aatcgccacc agcggcgttc cggcacgagc
     8041 ggttatcacc agctcggtcg ggtcgtaatt aacaatgccg cgatgacaac gcacatccag
     8101 cgtttgcccg gtgactgggc gacctaaaaa ggctttgcta ttgctgccct gaatcaccag
     8161 cggcgtttta tcgctaattg cctgattcac ctgctccagc agcgcctgac tgtaatcaca
     8221 ctcgcgtagc atcaaaagcg ctccagttca ggaaaaggta aatgaccgtg atgcacatgc
     8281 atggcaccaa attcagcaca gcggtgtagc gtgggaatgt ttttcccagg gttcagcaaa
     8341 ccatcggggt caaacgccgc cttgaccgca tggaaggtcg tgatttcatc gctgttgaac
     8401 tgggcgcaca tttgattgat tttttctcgc ccgatgccat gttccccact gatgctgccg
     8461 ccaacttcaa cgcagagttc gaggatcttc ccgcccagct cttccgcgcg ggcaaattca
     8521 ccaagttcgt tggcatcgaa aaggattaac gggtgcatgt tgccatctcc ggcatgaaag
     8581 acgttggcaa cacgtaaatc atattgctgc gataaacggg caatgccttc cagtacgcca
     8641 ggcagggcgc gacgcgggat ggtgccatcc atgcagtagt aatccgggga gatacgtcct
     8701 accgccggga acgcattttt gcgaccggcc cagaaacgta cgcgctctgc ttcgtcctgt
     8761 gccaaacgga cgtcagtcgc gcccgctttc aacaagatgt agttaacccg ctcgcagtct
     8821 tcctgtacgt cagactccac gccgtccagt tcgcataaca aaatcgcttc ggcgtcgacg
     8881 ggataaccgg catgaataaa atcttccgcc gcgcggatcg acaggttatc catcatctcc
     8941 agcccgccgg ggataatgcc attggcgatg atgtcaccaa ccgcaagtcc ggctttttct
     9001 accgagtcaa agctggctaa cagaacccgc gccacgggcg gcttcggcag cagttttacc
     9061 gtcacttcgg tggtcacgcc gagcatacct tccgatccgg tgaacagcgc cagcaggtca
     9121 aaaccaggtg aatccagcgc gtccgatccc agcgtcagcg cctcgccgtc cagcgtttgc
     9181 acttcaattt tcagcaggtt atgtacggtc agaccatatt tcaggcagtg gacgccaccg
     9241 gcattttccg ccacattgcc gccaattgaa caggcgattt gtgaggaagg atccggcgcg
     9301 taatagagat tatgcggcgc aacggcctgg gagatcgcca ggttacatac gcccggctgc
     9361 acgcgcgcgc ggcgaccaac ggggttaatg tcgaggctct ctttaaagcg cgccatcacc
     9421 aacaacacac ctttttccag cggcagcgcg ccaccagaaa gcccggtgcc tgcaccacgg
     9481 gtcaccaccg gtacacgcag gcgatggcag acagccagaa tcgctgtcac ctgttccatt
     9541 tgcttaggca gaacaaccag taatggacgc gtgcgatacg cgctcaaccc gtcacactcg
     9601 taaggaatga tctcctcatc ggtatgcagg atctcaagtc cagggacatg ctcacgcagt
     9661 gccatcagta ccgatgtgcg gtcgacatcg ggtaaagcgc catcaagacg ctcttcgtac
     9721 aagatgctca tgagtaggct tcgctttgtt gtgtggcagc tgatttttgc gcgctgcttc
     9781 tgtgaacagt tattaagcgg gcttttcgtt ttcgtctatc tctttagcta ccggtcagac
     9841 catttttttt ccagctctgt gaccttgtct tggttaactt aatgttaaat tgatgtaaca
     9901 taatcacttg cgtgatgtgc gtgttttgcg agttaagaac agaaaaattg gtcctacctg
     9961 tgcacgaggt ccgggaatga aagatgaacg tcgccctatt tgcgaagtgg ttgcagagag
    10021 tatcgaacgg ttaattatcg acggcgtact gaaggtcggt cagccgcttc cctcggaacg
    10081 tcgactgtgt gaaaagctcg gcttctcacg ctccgcactg cgtgaagggc tgaccgtgct
    10141 gcgcgggcgc gggattattg aaacggcgca gggtcgcgat tctcatgtcg cacggcttaa
    10201 tcgggtgcag gacaccagcc cgctgatcca tctgttcagt acgcagccgc gaacgctgta
    10261 cgatctgctc gacgttcgcg cattactgga gggcgaatcg gcaaggctgg cggcaacgct
    10321 gggaacgcag gctgattttg ttgtgataac ccgctgttat gaaaaaatgc tcgccgccag
    10381 tgagaaccac aaagagattt cgctgatcga acatgcgcag ttggatcacg ctttccatct
    10441 cgccatttgt caggcttctc acaatcaggt gctggtgttt acgctgcaat cattgaccga
    10501 tctgatgttt aattcagtgt ttgccagcgt aaataatctc taccatcgac cacagcaaaa
    10561 aaagcagatc gatcgccagc atgcgcggat ctacaacgcg gtgttgcagc ggctgccgca
    10621 cgtcgctcag cgcgcagcgc gcgatcatgt acggaccgtg aaaaagaatc tccacgatat
    10681 cgagctggaa ggccaccatt tgattcgctc ggcggtgccg ctggagatga acaaggtggg
    10741 aatgtgagca gagttttacc ctatccccaa ggcgagggga ccgcccctgc acttaacagg
    10801 atctggtgtt aaatctgttc atctataccc tctcccgaat gaccgcccct gcgctaaaca
    10861 ggatctggta ttaaatttgc ttttcataca atcggtaacg cttgtacggc tccgccccaa
    10921 tgcgttccag catgttattc atgcctgtat tggtttcgag gatccattac atctccagcg
    10981 catcgatctt ccggcgggca aacggatcgc gtaaggcttc aatcaataac agcgcaatca
    11041 ccgggccgat gcggctgaac tgatactcgt cgcgcacgcc catcagcggt actcgcgcag
    11101 ttcgcacacc gctgactttc aaacgccaca gcaattttgc ccagccgaag ggaaagagcg
    11161 atccgttcag atcggcaatc gcctcgttga tgttaggcaa gccgacaata aacgcgcagg
    11221 gtgcagaatc aatctcagcg atgtagatca tatcgtccgg caccagatat ttaagttggt
    11281 cgcccatggt cgcgaattca tgttcggtaa acggcacaaa tccccagttg tgctgccagc
    11341 cagagttgaa aatctcacgc aggatctgca tctcttcggc aaaccgctga cgattgatgc
    11401 agcgaatggt cacctttttg cgcacctgat ccatcagttt ttttagcgcc ggagagaaag
    11461 tgagatcggt tcgctgcatc caccacgcca gtaaatcaat gcctttgtga taacccagtt
    11521 gttcaatatg cgcggcatac cacggtttgc cgtgtggcat catcgcacag ggtggtgtgt
    11581 caaaaccttc aatcagtaat ccgctttcct gattgatatt caggctgaaa ggcccgctga
    11641 tcttacttgc accttgtgac ttcaaccacg cttccgctgc gccaaacaac gcggcaaaat
    11701 cctgcggatc atcaatggcg tcaatcatgc cgaaatgacc ggtatcttca ccgtaacgct
    11761 cacggtgcaa ggtatctatt tgcgcggtaa tacgcccaac tatctgcccc gctttttttg
    11821 ccacccacgc ctgccagatg atatggtccg tccccggatt tttcgcagac aaatgctcgt
    11881 tgcgttcaat gaataaaggg ggtatccagt ttggatcgtc gggataaagt gatgacggaa
    11941 aagcgataaa tgccttaagg tcatttttat taattacttt ttcaatttta atcatcagca
    12001 gatcgcattc catatctctg tggataacaa ataattaatg gtgaaaaaca ggttgcaaca
    12061 gcaacgagcg gcatcttaat aatcaactca atgaatacaa acgacgaata aatagtatta
    12121 attaatgatt atgataaggt cagaggctac acagaaacgc ctcaatgcct tttgtcgatt
    12181 taaggcgacc ggacaaggct tcttcttaat atattcataa attaaatatc attcataata
    12241 aaaataaaaa actcaaactc attatttaaa tcaatgttca ttttcacacc gatgaaaata
    12301 ctatctcatt tatcttttat gcctatataa tccaccgcat tattgtcatt ttatttttga
    12361 tatccaattt tttcgtggtg tatatgtcta ataaaatctt tacgcattcc ctacctatgc
    12421 gctatgccga ttttccaacg ctggttgatg ctttggacta cgccgctctg agtagagccg
    12481 gaatgaattt ttatgacaga cgttgccaac ttgaagatca actggaatat cagacattaa
    12541 aaacgcgtgc cgaagctggt gcgaagcggt tgttatcgct gaacctgaaa aaaggcgaac
    12601 gcgtggcgct gattgccgaa acgagtagcg ggtttgtaga ggcttttttt gcctgtcagt
    12661 atgctggctt agtcgccttc ccgttggcga ttccaatggg cgtcggtcag cgggattcct
    12721 ggagcgccaa actctagggt ttactggcaa gttgccagcc cgcagccatt atcactggtg
    12781 atgagtagtt gccactggtc aatgccgcga cgcataacaa ccccgaatta catgttttaa
    12841 gccacgcttg gtttaaggca ttaccggaag ccgatgttgc gctccagcgt ccagttccga
    12901 acgatatcgc ctacctccag tacacctccg gcagcacccg ttttccccgt ggcgtcatta
    12961 tcacccatcg cgaagtgatg gctaatctac gtgctataag ccacgatggc attaaattac
    13021 gccctagcga ccgctgcgtc tcctggcagc ctttctacca tgatatggga ctggtcggct
    13081 ttctcctgcc ccccgtcgcc acgcaacttt cagtagatta tttgcgcact caggattttg
    13141 ccatgcgtcc tttgcaatgg cttaaattga tcagtaaaaa tcgcggcacc gtttccgttg
    13201 cgccgccgtt tggctatgaa ttgtgccaac gccgcgtgaa tgaaaaagat ctcgctgaac
    13261 tggatctttc ctactggcgc gtcgctggta ttggtgctga gccgatctcc gcagaacaac
    13321 tccatcaatt cgctgaatgt ttccgtcagg ttaactttga caataaaact ttcatgccgt
    13381 gctacggact ggcagaaaat gcgctggctg tcagcttctc tgatgaagcc tccggggttg
    13441 tggttaacga agtggatcgc gacatcctcg aatatcaggg taaagccgtc gcgccgggtg
    13501 cagagacacg cgccgtatcg actttcgtca actgcggcaa agcgttgccg gaacatggta
    13561 ttgaaatccg caatgaagca ggtatgccgg tcgcggaacg tgtggtaggc catatttgca
    13621 tctccggtcc cagtctgatg agcggttact ttggcgacca ggtttcgcaa gacgagattg
    13681 ccgcgacggg ctggttagac accggcgacc tcggttatct gctggacggt tatctgtatg
    13741 tcaccggacg cattaaagat ctgattatta ttcgtggccg taatatctgg ccgcaggata
    13801 ttgaatatat agcggaacag gaaccggaaa ttcattctgg cgatgcgatt gcttttgtta
    13861 ccgcccagga aaaaatcatt ttgcagatcc agtgtcggat cagcgacgaa gaacgtcgcg
    13921 ggcagcttat ccacgcgctg gcggcacgga tccaaagcga atttggcgtg accgcggcta
    13981 tcgatctgtt gccgccccac agtattcccc gaacgtcctc cggcaagcct gcccgtgcgg
    14041 aagcgaaaaa acgttatcag aaggcttatg ctgccagtct taatgtgcag gaatccctgg
    14101 catgaatcaa actgtcgcgg tgacgggcgc taccgggttt atcggtaaat atattatcga
    14161 taacctgctc gcccgcggct ttcacgttcg cgcattgacg cgtactgccc gcgctcacgt
    14221 caatgataat ctcacctggg tgcgcggttc gctggaagat actcattcac ttagcgagct
    14281 ggttgccgga gccagcgtag tggtccattg cgccgggcaa gtgcgcgggc acaaagaaga
    14341 gattttcacc cgctgtaacg ttgacggcag cctgcgcctg atgcaagcag caaaagagag
    14401 cggcttttgc caacgttttc tgtttatctc ttcgctggcg gcgcgccatc ccgagctctc
    14461 ctggtatgca aaatccaaat acgtcgccga gcaacgactg gcagcaatgg cggacgaaat
    14521 tacgctgggc gtttttcgcc cgacagccgt gtatggtccc ggcgataaag agttaaaacc
    14581 gctgtttgac tggatgctac gcggcctgct gccacgactt ggtgcgccag atacacaact
    14641 ctcttttctt cacgtcaccg attttgcgca agcagtgggt cagtggttaa gcgccgaaac
    14701 tgtacagacg caaacctatg aattatgcga tggcgtcgcc ggtggctatg actggcaacg
    14761 cgtacgacag cttgccgcca aagcccgttg tggttccgtg cgaatggttg acattcctct
    14821 gccggtactc acaggccttg cggatatcag taccgcgttg agtcgcctgg caggtaaaga
    14881 acctatgctg acccgctcga aaattcgtga attaacccac gccgactggt cggcaagtaa
    14941 taaccgtatt tctgaagata tcaattggtt tcccgggatt agcctggaac acgcattacg
    15001 caacgggcta ttttgattgt cttgttttaa aggtgtgtta acgtttctat ggtaaatcgt
    15061 gaaatagtaa tggattacat cctttcctgt ttacaggatt tggtcgaaaa cggtgtggaa
    15121 attaagcccg acagcgatct cgtcaacgat cttggcctgg agtcgatcaa agtgatggat
    15181 ctcttaatga tgctggaaga cagattcgat atttccattc ctattaatat tctgctgaat
    15241 gtcaaacccc ctgcgcaatt aatggaaact ctacttccat ggctggagaa taaataatgg
    15301 ggctatacga taaatatgcg cgccttgctg gcgagcgtct gcaatttagt gacaacgggt
    15361 taacaccgtt tggcacctgt attgatgaag tgtattccgc caccgaaggc cggatcggca
    15421 ataaaaaagt gattctggcc gggaccaata attatctcgg tttaaccttt aatcatgatg
    15481 ccatcagcga aggtcaggcc gcgttagcgg cccaaggcac cggcaccacc ggttctcgta
    15541 tggcaaacgg cagttatgcg ccgcacctgg cgttagagaa agagattgcc gagtttttca
    15601 atcgccctac cgctatcgtt ttttcaaccg gttataccgc taatttaggc gtcatcagcg
    15661 cacttgccga ccataacgcc gtggtgttac tggatgccga cagccacgcc agtatttatg
    15721 acgcctgttc tctcggtggc gcggaaatta ttcgtttccg ccataacgat gctaaagatc
    15781 tggaacggcg catggttcgc ctcggtgaac gggcgaaaga ggcgattatc atcgttgagg
    15841 gcatctacag catgctcggt gacgttgcgc cgctggcgga aattgttgat atcaaacgtc
    15901 gtctcggtgg ttatttgatt gtcgatgaag cgcattcgtt tgtcgtgtta ggcgcaacgg
    15961 ggcgcggact ggctgaagct gtcggcgttg aagatgatgt ggatattatc gttggtactt
    16021 tcagtaaaag tctggcctcg attggcggtt ttgctgtcgg ttcggaagcg atggaagtgc
    16081 tgcgctacgg cagccgcccg tatattttta ccgcctctcc ttctccgtcc tgcattgcga
    16141 ccgtgcgctc gtcgttaaga actattgcca cacaaccaga gctgcggcaa aagctgatgg
    16201 ataacgccaa ccatctgtat gacggtctgc aaaaactggg ttatgagtta agttcacata
    16261 ttagcccggt agtgccagta ataatcggtt cgaaagagga aggtttacgc atctggcgcg
    16321 agcttatctc gccgggcgtg tatgtgaatc ttatcctgcc gcctgccgcg cctgcaggca
    16381 tcacgctgct gcgctgtagc gtaaatgctg cccacagtcg tgagcaaatc gatgccatca
    16441 ttcaggcatt tgctactctg aagcagtaat gtgagcccgc cgtggtgttt gcgccacggc
    16501 gggtttaatg ttgtctgtca taccaataca ccaggctcaa cgccaccaac acaaacgcta
    16561 ccggtaaacc gagcgcggta acaggtgcgc ttaacgcaaa tagcaatccc agattaacca
    16621 tgatttgata gctgatccag gtgagtaagc ccacgatgac gcctacagcg agacggctac
    16681 ccatcccagg cgaacgcggg gcactaaagg taaacggcac agccagcaaa atcatcgcca
    16741 gggtcaggat gggttgcccc agtttttccc acagagcaag gcggtattcg ctgctgggtt
    16801 gcccggtttc ctgcaaataa tggatgtaat gattaagctg acggacagaa aaagtattcc
    16861 ccggcattga taactcttca agattcatac tggtgaaggc cgattgccag gcaagattat
    16921 ctaatgtttc cagcgtttct ttgccattca gccatttttt atgattcaca ccatgcagga
    16981 cccacgtttt gtcgtcttca atggtggcac tgcgtgcata aatgtaggat tcaagcgaga
    17041 gatcgtcgcg ataatgaaat atctccacgc ccaccggctg gccctgctcg ttcagcgatt
    17101 tcactgtcac aaattcatta ccacgcctgg cccacagcat attgccggta atgtcatcgt
    17161 cctcccccaa cgcggtggcg gtcgatttga tttgcaacgc ctgttgctgc aatggcgacg
    17221 ccacccactc atctatcgcg cctaacgaaa cagtccacaa tatcccggca accagcgcca
    17281 ccagtgcaat acggaagata gaaaaccccg tgctgcgaat ggcggtaagc tcactgtttt
    17341 tcgataactg ccccagtcca acaataccac ccaataacgc gataaacggc gaaagttcga
    17401 ccagtgtgcg cggtaaggtc attagcacca ccagcaccgc ctgagtccag cgataaccgc
    17461 ccgggctgac accatccagt tcgttaatca ggttgaaggt ggtaaaaagc ggcagcaata
    17521 gccctgcagc ggcggcaaaa ccgagaaaga gatgacggat taaatagcga ctgaaaacat
    17581 tcatcgggaa aatttctgca gcaaagagaa gtcacgtgcg accagtatca gcaggcccat
    17641 tagcatgagt ccgggcacta accacaaacc aggaatgagg ggaatcgcgc cgttagccac
    17701 cagtgtacgg cagacgtcgc cgccataaaa aatggcaata aacagcaacg tcagtggcaa
    17761 taacgttgaa aatcgccctt gccacggttt tacccgactt aatgaaatgg ccagcagcgc
    17821 catcaacaat gcggtcaggc cacggctttg tcgccattgc agttcggcat gatcggcagg
    17881 aaaggcggag cgcgccagct ccgtgaccga tttcgctttg cgtttgacgt tcgggctttg
    17941 atccagcgga ttcagatgta attgcagatt acggtagatc tgttcgttgt cgtcacgccc
    18001 ctgatgatcg agaagatagg cagtgccgtt gtgcaacata acggtcggtt tctcgggtga
    18061 tgggtcaacc acatcaaccg aacgggcgcg gaaaatacgg gttcgattgg cagtagaagt
    18121 ataaatcagc gcgtcagtca ggcgattatt atcctgatca accgtctgcg aaaggatcat
    18181 tcgtccgtta tcgttagtgt taaatttctt tgcccgcaac tggcgaacat ccagctccga
    18241 ctgcgactgc tgctccagtt gataaatctg cgcataagcc cacggacgac catacatcga
    18301 cagaagggtg acaaaaatac ttagcggtat cgccagataa agaacggctt tgtacaagcg
    18361 tcccggactg ccccccgccg cagagatagc ggtaatttcc gagtcggtat acatctgccc
    18421 tagcgtcacg ccaactgaca catacagacc aacaggtaac aacatctcca gtgcaatcag
    18481 tactttgtag aaaacgatat ccagcacaac atcaagagct aatgtcccat ttgccgcttc
    18541 ggtcagataa cgctgtgcgg agtagctggc gaaaataaag atcagaaaac cgacgataat
    18601 cagcaccaga cggcgcgtac cacgcatgat gtagtgttca accagcttca tctcactaac
    18661 gccctcaatc ctttgagttt tttattcatt gctcagtttc tgcgccaacg caatgacatg
    18721 atctacctct tccggcgtca ggctgccgtc ctttgcccac tgaatttgcc cggctttatt
    18781 cagcaccaga atggttgaac tctgctccgg caatcgccag gcaacgcgtc cctgtccatt
    18841 gccgtcaata acgaattgcg cccacggata gcggcgttta tttttttcga ttttgccaac
    18901 aacaaaatag ccagtaccaa agatggcatc atcggtgttc acaatggtgg tgggctgaaa
    18961 acgatcctgc ggaaagtttg ccgcttcaac agctttaatt aacagtgagt tctttttctt
    19021 cgccgactta cgcccggcga tatactgaat aatgcggact tttcccgcta attgtgtgct
    19081 gttccacttt tggtagtgaa tttcgtcgtc gctattcaga agcagctcgc cgccttcctg
    19141 gatataaaca ggggtaaccg ttttgccagt gacgaaattg tgtgcctgca cacccggggc
    19201 taagagcatg ataatcaacg caataatcaa acgagatgac atcattgctc cggctatagc
    19261 ggaaaacgcg aaatcagcag ctctaagcgc tgacaaatat cgctatccag cgccaacggt
    19321 gcataatacg actggcggtt ttccatatca tccgccacca tgtcgtcgtg tttatctccc
    19381 atcaggccat taatggcggc gaggaaacgg gaaatctgta acgcctcttt attttcagga
    19441 tcaagcgatc ttaaggtgtc gtaataagaa acctgctggc tgatgatttt tcgcacatca
    19501 tgaaattccc tgccggttag tcgcggtttg gcaataatct ctttcagggt atgcatttgc
    19561 ttttgccgat aggcaataaa actgcttacg ctttctaact ggctatggcg taatgaatag
    19621 ttcaccaacg accagaccgg agagcttaaa taaatccgta acaggttgcc gtaatacgac
    19681 acaatatttt tcttaccgtt acgaaagccg tcctgaaaac gccccagaaa aacggtcgtt
    19741 ttgccgaata aaaatccggc ctgatgcttc tttaaataca gccgttgcgc aaaacgcagg
    19801 tgcttgtagc gcgctcgcat gtatttaaat tgtttgcgct catcagcagt cagctcatcg
    19861 cctttggctt gtttgcgaac taaacgcagt aattcctgac gatcaacgcc attgagccag
    19921 aattgcaggc ataacgcgta attatcatga atgatttcgg tggtacaact tagtgcaata
    19981 gtcggcggca aactaacctg tgcatcaacg acatcatctt cttcaatcgc aacaaataga
    20041 cgctcgctcg cctcaagcgg gaaatatttt ctcatcatca ggatttctct ccggctgcgt
    20101 tataaagcaa ggaatggcgc tgaaaaaatg cctttaaggt tggaattaac gagagcgcca
    20161 cgtccagaca ttgcccaccg aagtaagcaa acgctgctcc cagcacacca aacagatgac
    20221 agaacatgaa cagcaatacg atgtagccga ttgaggcgat ggtttgcgcc acgagaaaag
    20281 cgcgctgttt tcccgccatc aatagcaatg actcctgcgg gaagcccagc atcgaaatca
    20341 caattgcgcc cagcatcacc tgaatcaggt catacgcttc gagatactta acgccaaaca
    20401 ccagcgaaat gagcggcttg ccgacgatca acaccgcgag cgccaccaga ataccgattc
    20461 cgcccgccag taaaccggac ttcacaccta acaaccacgg tctggtggtg cgcggatcta
    20521 aacgcatcac ctctgggtaa aaacttttac ccagcaaacc cgctggcgtt ccggcggcgt
    20581 cgaagaatgt catggcgatt ttaaataacc cagcggcagc gggtcctaac acgatcccca
    20641 ccaacactgt gctgcacgag ttacgcgccg accagatgga gtgggcaatg ttggttgacc
    20701 agacaaaact ccacgcgcct ttaatgtgtc gggcagactc aaacagattc aatttgaagg
    20761 cgttatggat atttctacgg cgtaattcgc gcgcggcaaa ccaccagtac atggtgccgc
    20821 caaccagatt cgacacgtac caggcaataa caaaacccgc aaaaccaaag tcaaaatacc
    20881 aggctgcgac gctccccgct gcgcgcagaa aaggtttcgt cgcctgctgt acagcaatca
    20941 aatcaaagcg atctaccgca cgcagaatgc cggtcggcgt ggaggaagcc attgaaggaa
    21001 tgagcgtgca atacagcgcc gccagccaaa agctttggtc atccagacct aatgaatggg
    21061 aaaggaatgg cagtaaggca atgccgccga caatcgccac cgcgccgctg acgatatcca
    21121 gcgagaagga aaatgagacg acattgcgaa attgctgcgg attattgttg gtcaaggctg
    21181 gcgttccgta ctgaaccacc agctgccatg tctgaaactt aataaaatcg ctgatcgact
    21241 tggcgtacga ttgcacaatc accagtacac caaacatggc gggcgtcatc cctttaccgg
    21301 cacacgagag cgccaacagc cccagcaagg cgctctcgac attactggag cctaaccagg
    21361 cgctattgcg aataatggtg cgaaacgcgc catctgcaaa ccaatgtttg atgttaaaac
    21421 cagccaattc agcctgacct tatcgttgat agtaaaaaat atcccgccag ccttaagtta
    21481 aacttcggcg gtcagaaacg atggcaacca gagaaaccgc cttctgtacc tgttccagca
    21541 cttcgctgta gggcgctctg gaatcaatct caagaatttt tgtgccgtta tagccaatcg
    21601 tcgacatgac gccgattttg tcctgcagct cggcatactc atggtcaggc ttgcgggaga
    21661 tggcagtatc aatatcaatc cccaaacgaa tgattaattc aggacggtat tgcgccattt
    21721 tttggtataa actccgttcg cgctgcgcca gaaacatgct gattttcccg gtcgcacgtt
    21781 cgacgccgat ccccggtccg tcataataaa agcccgaaat ttcagcctgc gggaagcgat
    21841 cgctgaccac cagaacgcca ctttgcgcca gtcgctgaac cttgcgtaga ttcgccattc
    21901 ttcgcagcga gaagcagtac ataatcaccg ccgcccatag cgccggagat ttggttttca
    21961 tgctttgggt tttcgaggat ttggccgcca gtcgccgttc cagccagacg ccaaccaacg
    22021 gcaatcgttt gattttgtct ccgtcttcgc cggagagcag ccccagatag cggcgctcgg
    22081 tttgccagtg ttgttgcagc gatttcacca ggtcggtggt cagcgtggat ttaccggtgc
    22141 catcacaccc gaccaccgca atcagccccg gaatgtagtt gggctgcggc gcggttgtac
    22201 tattaacagt ttgtgtttgt agtgcatcca ttcgttaaat catcccctat ccattttggt
    22261 gatcaggaaa gcgcggcgtg aattgcgcgt agtgacgctt gcataatttc atcggctggt
    22321 tgccgaccat ccagttcaag gatcttggcg ccattgaatg tcagttgcgg cgtaacggcg
    22381 attttttcct gtaacgctgc cagttggtgg tcaggtttac gcgcaaacgc ggtttgttca
    22441 tcaatgccaa gacgaatcaa caatacgggt aaataagacg ccatccattg gtacagcttc
    22501 aactcgcgct gccttaacat ttttatccaa ccgttaccgc ccgtggtttt tgccaattgc
    22561 gggccatcaa agcgaaaacc cggcacttca acctgcgggt agcggtcggt gatgagcaga
    22621 tagccttgct ggcttttaca caacattttg cgaaacttgt acgcccgcca gcaggaaagc
    22681 agatagataa ccagcgcagt aatattgcca ggcggtgttg agggcttttc gtgtacatgt
    22741 gccgctttac ttcgcagata acacccacaa ggtgcgccaa taacagggag ctgtgaaatc
    22801 cattcgccaa ttcgcccgga cgattgcccg agataaatgt gttctgttgg cattcttgct
    22861 gccagttcat ttaccaggct tgccgtgagg gtcgatttac ctgaaccatc acatcctaca
    22921 atggcaataa cacgcactgg cgtggaatta attattgaca tattacgttg attcacgaaa
    22981 aacccggcag taaataatgt attgaatatt acgtggtccg tgctcagaat atccgttcaa
    23041 ccttattgtg acaatggtta attgtgacat tgacctgaat tacgctttca taaaaacata
    23101 ttaaccaaat aaatattttt aatggatatt taaattaaag gatatattca tgcagtcaat
    23161 aacacctcca ttaattgccg ttattggtag cgatggttca ggcaagtcaa cggtgtgtga
    23221 acatcttatt accgttgtcg aaaaatatgg tgctgccgaa agagttcatt taggaaaaca
    23281 ggccggaaat gtcggtcgtg cagtgacaaa attacctttg atgggaaaat ccttacataa
    23341 aacaattgaa cgaaatcagg tgaaaacagc aaaaaaattg cctggaccag ttccggcgct
    23401 ggtaattaca gcgtttgtcg cccgtcgctt actgcgcttt cgtcatatgc ttgcctgtcg
    23461 tcgtcgcggg ttaattgttc taaccgaccg ttatcctcag gaccaaattc ctggcgctta
    23521 cgatggtacg gtgttcccac ctaacgttga aggtggtcgt tttgtctcat ggctggcaag
    23581 ccaggaacgt aaagcgtttc actggatggc gagccataag cctgatctgg tcatcaaact
    23641 caatgttgac cttgaagttg cctgtgcacg taaacccgac cataaacggg aatcgctggc
    23701 gaggaagatt gccataacgc cacagttaac ctttggtggt gcacaactgg ttgatatcga
    23761 tgccaatcag cctctggaac aggtgttggt tgatgcagaa aaagcgatta cggattttat
    23821 gaccgcgcgt ggttatcact agtcaaaaat ggaaatgccc gatcgccagg accgggcatt
    23881 ttcaggaagg ttaaatcaac tgcaatgcta tccagtacag cccaccagaa agaaaaattg
    23941 ccgccggtaa agtaaacacc cacgccatca ggatgctggt taccgtttta cgctgtaacc
    24001 caccgccgtc caccaccatc gtccctgcta ccgcagacga gaggacgtgt gttgtggaga
    24061 caggcatccc aatataactg gcaaggccga tagacactgc cgccgtcatt tgtgccgcca
    24121 tgccttgcgc atacgtcatg ccgcgcttac caatcttctc accgatggtc atcgctacac
    24181 gacgccagcc aatcatggtg ccaatgccga gcgccagtgc taccgccatg atgatccaca
    24241 ccggagcgta ctcaatggtg cttaacatat cgctgcgcag tttcttcagc aggttctggt
    24301 cttctttact gacgcctggc agtttcgcca gcttcgcgga ggtatcagag atgcacagca
    24361 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
    24421 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttgccgga tgacagtgaa
    24481 actctgttac ttgcgtgcca tcagtcgatg ctgcaggcaa tggaggttcc atggcaatca
    24541 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggta acggcatcgc
    24601 gggtacgggt aatttcatag ccggacgcat tcatattgac gacgaagcca gcaggggcaa
    24661 tccccaccag taccagcatt accaggccga tccctttctg cccgtcgttc gcgccgtgcg
    24721 aaaacgccac gcctgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggtttac
    24781 gtttgccctt tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
    24841 tcccactcca gaaacggcgc agcaggaaaa tcagaccacc cgcaatgacc aggccgacga
    24901 taggggaaac aatcagcgag gagaaaattt tggttacttc acgcaggttt aacgcatcca
    24961 tcaccgatga gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcaccaatca
    25021 aggtgtgcga actggaggcc ggtaaaccaa agaaccacgt tcccaggttc cagataatcg
    25081 ccgccagcag catggaaaag accatcgcca gaccgtgagt tgaccccata ttcagcaaca
    25141 aatcggttgg taacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc
    25201 caaaaaagtt aaaaaatgcc gccatcacca cagcaagttg tggctgcatg gcacgagtat
    25261 aaataacggt tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca
    25321 acacaaatgc cagagcaagt aataacaaaa gccccgtata tatatcaagg ccaacaaata
    25381 aatttagcat atattagatt acgccatttt gaatttacga acggacgcat tatcagtgac
    25441 tttaacggca tgggcaaagt gaaatatcat tttttaacga taaaaagagt gttatttctt
    25501 gtgtattaga caataagcat tcattcaaaa cctttagaaa taatcaaaac acaggtattc
    25561 cactggttaa tatattcaca tatgaaatga ataaatattt gaatatataa atattgaata
    25621 ttttgattaa tccccgtact aattattctt cataatcagt acggggttgc tacaacatgt
    25681 attacttttt caccacaatt aacggttcaa tatcactctc ttttttgatg accagtgatt
    25741 catcaccgcg caaacacgtc ccaccgtagt tgccgccaac ggtgaaggta catacctgaa
    25801 tgtatttacc gtccactttc ggcaaacacc acagttgctg atagatgttt ttctgctcgg
    25861 caaatttacc gctggttttg tccagcacct cttcatgatg gccgaccagg tcgatattgc
    25921 taccacagcg accggcgatc ggtttcactg cgtaacctgt tttcaccaat tcatcattaa
    25981 cagtgaaatc ggtatccagc aggtaacgat ggtgcgggaa cagcgaccag aggatcggca
    26041 gaatcgcttt gttgccgggg atcaccgtcc acagcggctc aaagaccagc acttccgggc
    26101 gcagcaatac gtcgataaga cgcacttcgt tttgcggatg accggtacgg attggcaccg
    26161 cagcaaactc acggtcgcta acttcacgaa tctgatcaaa cgcggtttcc cacgcccagg
    26221 ttttccacac gcagttaacc agtcgccctt ccccatcaat cagttgcccg gcagcatccc
    26281 agcccagttc atccagcccg cgcaagatac gcgtttcaaa gcccgcctgg tgcagcgcct
    26341 gctccataaa ctgcgcgtga tagttttcct cgatatcttt gtcctgcatg atatggacaa
    26401 acggacgtgc acgactgtgt ttccaggcac cagccagttc gttaatcagc ccttccgccg
    26461 gattgaagcc gttgccttta tagccctgct ccgcccaacg ttcgaggatc aagcccgctt
    26521 cggtatgaca ggaggcggag tcggcgttgt attcgtaaac cttcaggcca cgctcatcca
    26581 tgcagaaatc catacgacca gtgatcatat ggtgacggcg acgctgccag gagagacgca
    26641 aacgtggcca gaggattttc ggaatgtcga acagcgccag caggttgtca tctttcagca
    26701 ccttgtcggt tgcgtgaaga tacatcagat gcagctcgtt ggtggcttta attagctcct
    26761 gctcggcact ctcggtaatg gtgtagtaat gatacggatc ctgattgatc acctgaccgt
    26821 tggcctgcac ataggcgttt tgtagcggat ctttttcatc cagccattta ccgtcaaact
    26881 ggcctttgtt ttccagacgc gctccactga ttttcagcag ctcgcctgca atttccggct
    26941 gcggtaagct gtattcagta tcttccgtct ggatcatcca gcccagaatg gtggtgtcat
    27001 caaaagtgtc tttaagggta tagcagccgt tttcgaccac catctccagc tcgcgcgtcc
    27061 actgttgccc ttgcggcaac ggggaatgaa tcacgttctg ttccgcaata cggactttgt
    27121 tgccatgcaa ttgggtaatg atggcgacat ggccagtgtc tttaaattca ccgcctttat
    27181 cccagataag aagcgcaccc gcgaccggcg cacgcggcga gccgttagga aatgcctgca
    27241 atggcaggat gttgtcatta accacttctc gcaggaagcg cagcgagaaa atctcccacg
    27301 ccatacccac gtcagtaaag accacaccgt aattcagaaa gagaaaacgg cgagcaaatt
    27361 caacgcattg ccacttgtgg cccatatatt cgtcgtcgat atagctacgg aatacggcgt
    27421 catcttcgta ttcctgcgga tcgagagaac tgtaatctga agagtagatt gctaccccgc
    27481 ctggggcgta gcccaataat gtcccgaacg gggcatcctg gctggtcgtt cctttgctca
    27541 tcactttacc tttaacaata caacctaagc agttggcgta attgttgtgc tctgattacc
    27601 tgctcagcga cattaaccgg acagaggtca gactaatcat acacctcatc gcagcggctg
    27661 gcgcaaaggg ttaaaaaatt cacattctgt acagcaagtg acctgctaca ctgcttcaac
    27721 actaccactc agaaggcaac tcactatgac agacaatact tatcagcccg cgaaagtctg
    27781 gacgtgggat aaatccgctg gcggcgcgtt cgccaatatc aatcgcccgt tttctggtcc
    27841 gacgcatgaa aaaacgctgc ccgttggcaa acatccattg caactttatt cgctgggaac
    27901 gccgaacggt cagaaagtaa cgattatgct tgaggagcta ctggcgctgg gcgttactgg
    27961 tgcagagtac gacgcctggc tgattcgtat tggcgatggc gatcaattct ccagcggctt
    28021 tgtcgaagtg aacccaaact cgaagatccc ggcgctgcgc gatcatacgc ataatccgcc
    28081 gatccgcgtg tttgaatctg gttcgatcct gctttatctg gcggagaaat ttggctactt
    28141 cctgccgcag gatctggcaa aacggactga aacgttgaac tggctgttct ggttacaggg
    28201 tgccgcgccg ttcctcggcg gtggttttgg tcacttttac cattacgcgc cggtgaaaat
    28261 tgagtacgcc atcaaccgct ttaccatgga agccaagcgc ctgctcgacg tgctggataa
    28321 gcaactggcg cagcataagt ttgttgcggg cgatgagtac accattgcgg atatggcgat
    28381 ttggccgtgg tttggcaacg tggtgctcgg cggcgtgtat gatgccgcag agtttctgga
    28441 tgcgggaagt tataaacatg tgcaacgctg ggcgaaagaa gtgggcgaac gtccggcagt
    28501 gaaacgtggg cgtattgtta accgcaccaa cgggccgctg aacgagcagt tgcatgagcg
    28561 ccatgacgcc agtgattttg agacaaatac agaagataag cgtcaggggt aagggttggt
    28621 gttcgtcgca gcaagccatc caggccggat aaggcgttca cgccgcatcc ggcaatcgtg
    28681 cacaatgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaagcta atcatcacgc
    28741 gctggtaatg tcgtaatcca tttggcgcag tgcgtctaat gtggctttgg cttcatcttc
    28801 gtcgatgatg ctcatggcaa atccgacgtg taccagcacc cactggccca gcagatcggc
    28861 agggttacct tcgcaaatca gggcgatatt cacgtcgcgc ttgataccac atacttcaac
    28921 ctgcgcaagc tggtgaatat cttcaccgac agccagcacc tggcctggaa cgccaataca
    28981 cattgttaac tccggttatt tcgattgccg gacaaacacg aaaaagaacc tttagcgggt
    29041 gttccctctc accctaaccc tctccccgga ggggcgaggg gcccgtccgt gcgcttttta
    29101 ggcgttgtcc tcagtcttat tcaacttcaa tacttttgac gatcagcgaa tcgccggtat
    29161 cgacacgcaa ccgctcgccg tgacagagcg gacactgcgc atcgtgctga tgaatctcca
    29221 ccacctggct gcaatcccag caccaggcct gggcgggttt atagacgata tgtaaatcgc
    29281 acccttgcgc caccgttccg tggcagacaa tttcaaaact aaaacggacg gcgctctcct
    29341 caacgcagga gagcgcgcca atttccagcc acacggcggt gacgcgctta acatcgtgct
    29401 gctccgcctg ccgttggata atttcaacgg cgctctggca aagcgacaac tcatgcattt
    29461 tcgcccctcc tgcggccaaa cagcagggcg cgacgccctg cgtgtggtac atccggattg
    29521 gtgactggca acgaaaggat cattcgtgcg cagtcatccg tcaggcgttg gccttcttca
    29581 atcgacatgc tgtgcgaaag cggcgacatc agcgaacagg agaggtattg cgaaacaccg
    29641 tccagttcgc caacggtaaa ggtcatggtg ccatacggca gttgcagacc aattttttca
    29701 ctcactttgc gcagcggcca gagttgatcc gggccgggga agataactgc actcagcatc
    29761 cacggggtga tcacacaccc cgtccactga ccttcgaaca gcgtaaaatc agaaacatac
    29821 accggcattg aaggatgcag aaaagagaga tcgtgcatcg aacgccgggc aatttcttca
    29881 aacgctgcct gtacttgcgc cttcggggag gtctggaaac ctgctatctc ttcagtcatg
    29941 aatcgcctcc cgtgggatgg cttccacgcc tgattcgcgc agcgcagcca gaaccagctc
    30001 aagcgcaggt tcaatcattg cttcaaccgt cggcgttaag ccgatgtgtg gctccagcga
    30061 ttccgggatc acgccgacca gggtcagctt tttcggaaac tcaccggtga agcgcagggc
    30121 cgacaatacg tcggccaggc caagctgatg cggagagatt ttgttggtaa acaacgccgg
    30181 aacttcttca tcccgcagga tcatcatcgt tcccggcgtg ttctttttcg acacaatggc
    30241 atccgcaata atcagatgat cgcgatttgc catgtcgcta agcagctcca ttcccgccgt
    30301 gccgccatcg aggatctcaa cataatccgg cagaatgtat cgttgctcta acgcttcgac
    30361 aatccgcacg ccgatggctt catcggtcag caaaatattg ccgaccccta agactaaaat
    30421 acgcattaca gaaccttcac tgaaaccact tcgttgccgt cggcatccac tacgtgtacc
    30481 gcacaggcca tgcacgggtc gaaggagtga atggtacgca ccacttccag cggtttattc
    30541 ggatcggcaa ccggtgtacc caccagcgac tgctcgtaag gaccgacgtc atcattgaag
    30601 ttacgcggac cagagttcca ggttgatgga acaaccgcct ggtagttgct gatgataccg
    30661 tctttaataa ccatccagtg agagagcata ccgcgcggcg cttcgaggaa gccaacacct
    30721 ttgaactcac ccgttgccgg aatgttcggc ttcacaaagg tggtgtgatc gcctttgccg
    30781 atattggtga tcagtgcact gtattggttt tgcaggatat cctgcaattc acagcagtga
    30841 acggtacgac caataatacg gcccagcgtg gagtgcagct gtgccacttc cagcgtgttg
    30901 ccagtcagtt tctgataaat cgcaacgatt tcattcagtt tgttctgggt agattcgcga
    30961 cctgccgcca gtttcaccag catattagcc agcggaccca cttctaccgt tttgccgtag
    31021 aaagtcggtg atttcaccca ggaatatttc ccgtcgtcag accaaccatc ataagccgga
    31081 atggtggtac cttcccacgg cgcctgtggc gcttcgtctt tataccagga gtgcttcgcg
    31141 ctttcctgaa tgcctttgat caggtattca tcggaatgag aagtgatcgg acgatacgag
    31201 gacagatccg cattctcaat gtagccgccc gggaacagga agctgccgtt tttgctgtcg
    31261 gtcgggaatt ccggcacgct caggtagttc accgcacctt taccgcgcgt cagccattcc
    31321 gggtagaacg cggcgataac cgcggtatca accttataaa cctgctcaac aaagtcgctc
    31381 agtttgtcga tgaaagactt gatgtacatc aggcgctcaa ggttcaacac gcccaacccg
    31441 tcgaggttga ttgggttcgc gacaccactc actgccaggt tctgaatgtg cggcgtttta
    31501 ccgcccagca gcgccacgac gcggttagcg tcacgctggc actccagcgc ctgcaggtag
    31561 tgcgctaccg caatcaggtt cacttccggc ggcagtttca tcgccgggtg accccagtag
    31621 ccattggcga aaatacccaa ctgaccgctg gcaaccagat ctttgatctt gttctgaact
    31681 ttagtgaact cttccggact gttcagatgc caggtcgaaa cgcctttcag catttcggag
    31741 gctttggttg ggtcagcttg cagtgcagaa gtgatgtcca cccagtccag cgccgaaagc
    31801 tgatagaaat gaacgatatg gtcatgcgtg gtgtgcgcag ccagaatgat gttacggatg
    31861 tattgcgcgt taaccggaac gtcgatattc agcgcacttt ctgccgcacg aacggaagac
    31921 agcgcgtgag tggtagtaca tacgccacag atacgttgca caatcatcca tgcatcgcgc
    31981 ggatcgcggt ttttcacgat ctcttccatg ccgcgccaca tggtaccgga agcccatgct
    32041 ttcgaaacga cgccattttc gatttcgcaa tcgatgcgta aatgcccctc aatacgggtt
    32101 accggatcaa tagtaattct ctggctcatg ctttgctcgc ctcatgacga ttatgatcgt
    32161 tttgttttaa aggaggaagt atcggcagta gacgaatgag tacgatgtaa gcgcaaatct
    32221 caatagccac aaaaccaata gaaatcaaca gttcttccca ggtcgggaag taggcgtaac
    32281 cgccgcccgg gttgaatgcc accaacgaat aggtcagacg ccaggttgca caacctaaca
    32341 gtgcgctcag tgctgacagg aacagcatgc gggaatcatt acgcagcttc gccacacgca
    32401 gaacgaccag cgggaagagc atcagcagga cttcaatcca gaacatcacg gagtagaagt
    32461 caccggcaaa cgctaacgac agcttgtcgc gatagataag ctcgccaaag cgcagcccga
    32521 tgaaaatcgc cagcaacaca ctgatggtgt tggtcagctt gacgaacagg ctcttttcat
    32581 ccggaccgtt gccacgcaga cccgcctgca ccagtgaacc ttcaaagatg acaatcgaga
    32641 agcccatgat gaacgccgtc agcagcgaga acagcggcaa catttcatag ctctgccaga
    32701 gcggatgcac cttgtagccc gccgagatca tcagcgaccc cattgaagac tggtgcatgg
    32761 tcggcagcag cgcaccgagc gcgatgatga agaacatcac cttgttcaga cgttgtagcg
    32821 acaccttcca gcccagacgt tcaaacagtg ccggagcaaa ctccagagcc atcacgccga
    32881 tgtagatggt catacagacc gccgtctcga acagtaccga gttcacgttg aagtgacccg
    32941 gaatgtagaa gtacggcagg ttccagtagc gacccacgtc gatagtgatc gacaagccac
    33001 ccagtgagta accaaacaga ctcgccaaca gcgccggacg caccagcgga tggtattgcc
    33061 cacggttaaa gacgtatacc gcccacgcca gcgcccagcc gccacaggca aagccggtgc
    33121 caatcagcag gtcaaacgcg atccacacgc cccacgggaa gccgccgttc aggtcagaga
    33181 cagagcccag accgaacacc agacgcttca caatcaggag catacagatg acgattaacg
    33241 gtccaaaaat catgaccggt ttactgatga ttttgccacc cagcggttgt ggatcatgac
    33301 tcatgatcgt ctcctccgtc gtgatggtcg tttttggtgt tgcgacgaac cagcacggtt
    33361 aagcccgcca gcacagccag tggtagcatc atgcctttat acagggtgtg ttgaatattt
    33421 tcggaacgcg caccggtaga aagatcgtcc agtttcggca ggtcgagatt ttcataaggc
    33481 acacccgtca gtaccagaac ctgagtaccg ccgccctctt tctcgccgta cagatgcgga
    33541 taatatttcg gcaccgtatg caggtaagtg tcgccagatt tcagcgtctg acgcggatag
    33601 tggtattcgc tgccaggctt cagcgccaga cgttttttcg cctccgccat cagctcttca
    33661 cgcgtaccga aaatcaccgc gcccgccggg cacacttcta cgcagccagg cagaccgcct
    33721 ttatcgagac gttccacacc tttctggttg cacagctcgc acttatgcag cgcaccaaac
    33781 gggttgttgt agtcgtactt cggcacgttg tacggacagg cgaccatgca gtaacggcaa
    33841 ccggtgcaca cgtctttgtc gtaatggaca atgccggttt tcggatcttt tttcagtgca
    33901 gagaccgggc acacagagac acagttcgga tcgacgcagt gcatacactg tttcttaatg
    33961 tacgcgtagc cgttctcctc ctggtctttg ttgacccctg tgccgctggt ccacacctga
    34021 atgatgttat tggtatacgg cgacagtttg tcgttgttcg accaggtctg ttccccttgc
    34081 gggttacgtt cagggaagtt gatatcctga cacttggtga cgcaagcctg gcagcctacg
    34141 cacaaggtcg agtcgtacaa catccccagc gatcccggaa ttggcgggcg gttttcagca
    34201 gccgcatgac tgacagacgg cagcgcgccc gtcagcaatg ccccgcagga ggctgcttta
    34261 ataaaattac gtctgttcac ggttattctc cccgtgagtc agcgttatct ttcttttgct
    34321 gacgacccag ttcacgcacc gccatcacgc tgacaccggc aaccagccca accacaccgc
    34381 cgagcaaacc aatagcgcct gcagagacgt tgccgccctc tttagcgtta acatccggtt
    34441 tctgtgaacg cggagtctga ttttcgacgt tggcaagctg atggatgcct ttatggaagc
    34501 cgataccttt ttcgttacag ccatagcacg ggtgaccaat cgccaccggc cacacaccgc
    34561 caacatcgca gaattgcagc gttgagcagt tgccgtaagt ttctggccct ttacagccga
    34621 ggtggtacag gcaccagcct tcgcggtggc cttcatcacc gaactctttg gcaaaacgac
    34681 cagcatcgaa gtgcgggcga cgttcgcagt gttcgtgaat cagacggcca taggcgaagg
    34741 tcggacggtt tttgtcatcc agtttcggcg gtttgccgta agtgatgatg tgcgcaacgg
    34801 tcgcgaggaa gttgtgcggg ttcggcgggc agcccggaat attgataacg gttttgcctg
    34861 gcagaacttc ttgcaagctg actgcgccag ttgggttaac tccagctgcg gcaacaccgc
    34921 cccacgcgga gcaggaacca atagcgataa tggctgctgc gccttccgcc gctttgcgga
    34981 tgtgatccac aatcggctca ccggcaacca tgcaataaat accgttatct tttaatggga
    35041 tggaaccatc caccactaac acatactgcc ctttgtactt ctcgagagcg ttatgtttgt
    35101 tctcttcgac ctgatgaccg aaggcggcgg aaagcacttc gtgatattcc agagagatag
    35161 tctccagcac gaggttttct accgttggat gcgttgcacg aagcagagat tccgtacaac
    35221 cggtgcactc ctgcgcgcca atccagataa ctggcggacg ctgcgggtta gtaaccgatt
    35281 cggccatctc tgcagcggct ttgctactta accccatggt ggcggctaat gctgcacaaa
    35341 gcttcatgaa atcacgacgg ttaatgccgt gagaatggat gagggtgtta tctccagtca
    35401 tttatagtta ttccgttgcg aggacctggc atatattttg cctcaatcgc aaaatcaata
    35461 atgcgatcga tgcgccattt accacacatt tattatggtt atcgatatca tgatactgcg
    35521 gcggggcgct aaaacgaagg gaagaagcat tagtgtaatt aattaaagca agataatacg
    35581 tatgtttgat caattttcga ggcgataacc ggccataaaa aagcgcaacg tagaacagga
    35641 attattctca cgaaagaaat ccattaccat ctctttatcc agtccatagc ggcgcgtcag
    35701 aatacccgct tcccaggcgc taagctggcg atctccggtc ttttcgtaca tccggtgtga
    35761 gtagcctaat acaaaaccgc gtttataatc ggcacagaat tttgtcacat taaccgcact
    35821 gtcggcatgc gttgctttta atcccgccat taagccttta ccaaaatggt tattcatcgt
    35881 tcaccccatt cgttatatat cgccatatat agcgatattt taggcaaaga acgacttttg
    35941 cgcacgctat tttttcatac ttgtttaaga cgtaatcccg taagagataa acctatcagg
    36001 agagatattt atcgaaccat ttcagtgtcc tttgccaggc aagatcggcg gcagatttgt
    36061 cataacgggg cgtggaatca ttatggaatc cgtgattaac ccccggatag atataagcct
    36121 cataaacctt attattgtct ttcaacgccg cctcgtaagg aggccagccc tcgttgattc
    36181 gggtgtccag ttccgcgaag tggagtagta aaggcgcttc aatcttcgcc acatcggcag
    36241 tgggtgcctg acgaccataa aacggcaccg cgcaggccag ttccggatac gcgacagccg
    36301 ccgcgttcga tacgccaccg ccatagcaaa atccggtaat acccacttta cctgtcgctt
    36361 gcggatagcg ttgcataaac tcaattgcgg caaagaaatc attcatcagt ttggttggat
    36421 caacctgctg ttgcagctca cgacctttat catcatttcc cggataacct ccaacggaac
    36481 ttaatccgtc aggtgccagg gcgatatacc ccgccttcgc cactcgccgt gccacatctt
    36541 cgatatacgg attcagtcca cgattctcat gcaccacccc cacggctggc gttttgccgc
    36601 tcatttttgc gggtttcacc agataacccc gtacctcgcc gtgaccattt ggcgaaggat
    36661 acgtgatgta ctcagcaaca atctccgggt cggtaaactc tacctgagtc gccagcgcat
    36721 aatttggctt gagcaaatca aacaacgcta atgccgtcat cccgcccacc gcatacttcg
    36781 ccgcaaggtt gaggaactca cgtttcgaaa ttttcccgtg aacgtaatag tcgtagtaat
    36841 ccaacaactc ttgtgggaaa tctttggcgg ttaaacgcgg catcgttgca ttcctcaact
    36901 ggtgtttttt taagcaaagc ataagcgcgt gtttttgccc agtttttcgt cactctgtga
    36961 gccagactac gggatacgcg ctggcgaatc gctaaactag aaacattgtt tcgaaattga
    37021 acggtggaaa ggagaggtca tggtctggtt agcgaatccc gaacgttacg ggcagatgca
    37081 gtaccgctat tgcggaaaaa gtggtttacg cctgcccgcg ttatcgctcg gtttatggca
    37141 caatttcggt cacgttaacg cgctggaatc acagcgtgca atcctgcgta aagcgtttga
    37201 tttaggcatt acgcactttg atttagccaa caattacggg ccgcctccag gaagcgcaga
    37261 agagaacttt ggtcgcctgc tgcgcgagga ttttgctgct tatcgcgatg aactgattat
    37321 ctctaccaag gctggctacg atatgtggcc cggcccgtac ggctctggcg gttcacgtaa
    37381 atacctgctc gccagcctcg accaaagcct gaagcgtatg gggcttgagt atgtcgatat
    37441 cttttactct catcgcgtcg atgaaaatac gccgatggaa gaaaccgcct ctgcgctggc
    37501 tcatgcggta caaagcggta aggcgctgta tgtcgggatc tcctcttact cgccagagcg
    37561 gacgcaaaaa atggtcgagt tgctgcacga gtggaaaatt ccgcttttaa ttcatcaacc
    37621 ttcgtacaat ttactgaacc gctgggtgga taaaagcggc ctgctggata ccctgcaaaa
    37681 taacggcgtg ggctgtattg cctttactcc tctggctcag ggattgctga ccggaaaata
    37741 tctcaacggc attccgcaag attcacggat gcatcgtgaa gggaataaaa ttcgtggtct
    37801 gacgccgaaa atgcttaccg aagccaacct caacagcctg cgcttattga atgaaatggc
    37861 acagcagcgt ggacaatcaa tggcgcaaat ggcgttaagc tggttgctga aagatgatcg
    37921 cgtgacgtcg gtattgattg gtgccagccg cgcggagcaa ctagaggaga acgtgcaggc
    37981 gctgaataat ctgacattta gcaccgagga gctggcgcag attgatcagc atatcgccga
    38041 tggcgaactg aatctgtggc aggcgtcgtc cgataaatga cctgttaata acgggcgcgc
    38101 gaaccgcgcc cgaatcagat cagtgattat gacgagtcag tcggtcaaga tagcccatca
    38161 caaatgcaga aagcacaaac gtcagatgga taatgacgta ccacatcagt ttgttatccg
    38221 ggacattttt cgcatccata aagacgcgca gtaagtgaat ggaagaaatt gccacaatcg
    38281 acgctgctac tttgtttttc agcgacgttg cgtccatttt ccccagccag ttcagcttct
    38341 ctttgttctc ggagatatcc agctgtgaga cgaaattctc ataaccggaa aacatcacca
    38401 tcaccagcaa accgccaacc agcgtcatat ccaccagcga cagcaacacg aggatcaaat
    38461 ctgattccgc catcgagaag atattcggca gtacgtgaat aatctcctgg aagaacttca
    38521 gcgccagggc aactaacgcc agcgaaaggc caaagtacac gggggcaagc agccagcgag
    38581 aagcatacat tgcattttca aaaaaacgtt ccatacattc ctgtcgtgta gttaaaacgg
    38641 gcaacagtat atcgcaattg agtaaacagc acgcaacagc aaatcaacat atacgttaaa
    38701 cctttgtcgc ataattctga ggtttaggga tcttcctggt tcccttatca acgcatcgag
    38761 agcgtctacc cttaaaggac gttgaacctg aagggagaaa aacgatgtct catttaaaat
    38821 gacccgacca cgcagtatta cactggtgaa tatcccaaac agaaacaacc gacgccaggc
    38881 atccaggcga agatgacacc gataccggat tgcggcgaga aaacctatgt tggtagcgct
    38941 cgcctgaaag atcgtaaagc actggtgaca gggggcgatt ccggaatagg tcgcgctgcc
    39001 gccatcgctt acgcgcgaga aggggctgac gtggcgatca gttatcttcc cgtggaagaa
    39061 gaagacgctc aggatgtgaa aaagatcatt gaagaatgcg gacgcaaagc cgttctgctg
    39121 ccaggcgatt taagcgatga gaaattcgcc cgttcgctgg ttcacgaagc gcacaaggcg
    39181 ttaggcgggc tggatattat ggcgctggtc gccgggaaac aggttgccat tccggatatt
    39241 gcagacctca ccagcgaaca gtttcaaaag acctttgcca ttaacgtttt cgcgctgttc
    39301 tggctaaccc aggaagcgat ccccctgcta ccgaaaggtg caagtatcat caccacttcg
    39361 tcaatccagg cataccagcc aagtccgcat ttactggact atgcggctac gaaggcggcg
    39421 attctgaact acagccgtgg cctggcaaaa caggtcgcgg agaaaggtat tcgggtgaat
    39481 attgtcgcgc caggccctat ctggacggca ctgcaaattt ccggcggaca aacgcaggat
    39541 aagatcccgc agtttggtca gaaaacgccg atgaagcgtg cggggcaacc ggcggaactg
    39601 gcccctgtat atgtttatct ggcaagtcag gagtcgagct acgtcaccgc agaagtgcac
    39661 ggcgtgtgcg gcggcgagca tttaggttaa aaaaatgccc ggttgtgaaa agcaaccggg
    39721 catcattgtg aattcactct tacctgatgt ggcgaaaacg ccttatccgc ctacaaacgc
    39781 atgaagattc aacatgttgc aatagatttt gtaggcctga taagcgtagc gcatcaggca
    39841 ttgagcacct aatgccggat gcgtcgtgaa cgccttatcc ggcctacaaa cgcacgaaga
    39901 ttcaacatgt tgcaatagat tttgtaggcc tgatgagcgt agcgtatcag gcaattctac
    39961 tttacttcgc tttggcggtt tcttcgccga ccagacctat cttcaggtaa cccgcctgat
    40021 gcagcgtatc cattaccttc atcaacgtct cgtaatcgac ggttttgtcc gctcggaaga
    40081 agatggtggt gtctttcttg ccttcggtta acgcattcaa cgccgtaatc attgtttcat
    40141 cggtgaccgg atcgttaccg ataaacatcg agttgtctgc cttcaccgac agataaaccg
    40201 gtttttccgg gcgcggctgc ggcgtgctgg tagaagcagg caagttcacc ttcacatcta
    40261 ccgtcgctaa cggtgccgcc accataaaga taatcagcag aaccaacatc acgtcgataa
    40321 acggcgtcac gttgatatca tgcatttcgc cgttatcgtc gaggttttcg ttaagatgca
    40381 ttgccatcgg acattatcct gcgcgtaatt tttgtgcgac acgaaccgga tgcgcagcgg
    40441 cgctggcttc cagatccagg tcacggcttt gcagcaacaa tacctgcgct gcaacatcac
    40501 ccagcatcgc tttaaagccg ccaatctggc gtgcaaatac gttatagata acgaccgcag
    40561 gaatcgctgc cacgaggccg attgccgttg ctaacagagc ttctgcgata cccggcgcaa
    40621 cgactgccag gttagtggtc tgcgtttgcg cgataccaat aaagctgttc atgatgcccc
    40681 agaccgtacc aaacagacca acgaacggag aaatcgcgcc gatggttgcc agatagccgt
    40741 taccgcggcc catttgacga cccactgcgg cgacccgacg ctccagacgg aagctggtac
    40801 gttctttaat accttcgtta tcgtcgctgc cttctgacag ttccagctca ttctgcgctt
    40861 cattgagcaa atgcaggctt aagcttttgc taccaaaatc agcggcgata tcgttggcct
    40921 ggtttaagga acgtgcttca gccagcagtt gctgctcgcg cttaaggcga cgcttctgat
    40981 tgaagaactc tacgctctta ctgaagaaga ttgcccaggt gactacggag gccaaaataa
    41041 gcccaatcat cacgcactta acgacaatat cggcgtgctg atacataccc cagacggaaa
    41101 ggtccgtctg cattaaatta ttacccactg tgtatctcca ggacgcaagt cacaaaatct
    41161 gcgcataata atatcaaaac gacgtcgaat tgatagtcgt tctcattact atttgcatac
    41221 tgccgtacct ttgctttctt ttccttgcgt ttacgcagta aaaaagtcac cagcacgcca
    41281 tttgcgaaaa ttttctgctt tatgccaatt cttcaggatg cgcccgcgaa tattcatgct
    41341 agtttagaca tccagacgta taaaaacagg aatcccgaca tggcggacaa aaagcttgat
    41401 actcaactgg tgaatgcagg acgcagcaaa aaatacactc ttggcgcggt aaatagcgtg
    41461 attcagcgcg cttcttcgct ggtatttgag agtatggaag ccaaaaagca cgcgacgcgc
    41521 aatcgcgcca atggcgagtt gttctatgga cggcgcggaa cgttaaccca tttttcctta
    41581 caacaagcga tgtgtgaact ggaaggtggc gcaggctgcg cgctatttcc ctgcggggcg
    41641 gcagcggttg ctaattcgat tcttgctttt gtcgaacagg gcgatcatgt gttgatgacc
    41701 aacaccgcct atgaaccgag tcaggatttc tgtagcaaaa tcctcagcaa actgggcgta
    41761 acgacatcgt ggtttgatcc gctgattggt gccgatatcg ttaagcatct gcagccaaac
    41821 actaaaatcg tgtttctgga atcgccgggt tccatcacta tggaagtcca cgacgttccg
    41881 gcgattgttg ccgccgtgcg cagtgtggtg ccggatgcca tcattatgat cgacaacacc
    41941 tgggcagccg gtgtgctgtt taaggcgctg gattttggca tcgatgtttc tattcaggcc
    42001 gccaccaaat atctggttgg gcattcagat gcgatgattg gcactgccgt gtgcaatgcc
    42061 cgttgctggg agcagctacg ggaaaatgcc tatctgatgg gccagatggt cgatgccgat
    42121 accgcctata taaccagccg tggcctgcgc acattaggtg tgcgtttgcg tcaacatcat
    42181 gaaagcagtc tgaaagtggc cgaatggctg gcagaacatc cgcaagttgc gcgagttaac
    42241 caccctgccc tgcctggcag taaaggacac gaattctgga aacgagactt tacaggcagc
    42301 agcgggctat tttcctttgt gcttaagaaa aaactcaatg atgaagagct ggcgaactat
    42361 ctggataact tcagtttatt cagcatggcc tactcgtggg gcgggtatga atcgttgatc
    42421 ctggcaaatc aaccagaaca tatcgccgcc attcgcccac aaggcgagat cgattttagc
    42481 gggaccttga ttcgcctgca tattggtctg gaagatgtcg acgatctgat tgccgatctg
    42541 gacgccggtt ttgcacggat tgtataacat tgccactttt ggacaatttt gcagacattt
    42601 tattgtgaaa agtcttaaat tgttgcgtcc gggatcaagg cgtcccggac gattcaggag
    42661 tacaataggc agataaaagc ttaaacgctg ttccacagga aagtccatgg ctgttattca
    42721 agatatcatc gctgcgctct ggcaacacga ctttgccgcg ctggcggatc ctcatattgt
    42781 tagcgttgtt tactttgtca tgtttgccac gctgttttta gaaaacggcc tgctgcccgc
    42841 ctcatttttg ccaggcgaca gcttgttgat actggcaggc gcattgattg cccaaggggt
    42901 tatggatttt ctgcctacga ttgcgattct gaccgccgca gcaagtctgg gctgctggct
    42961 aagttatatt caggggcgct ggttagggaa taccaaaacg gtgaaaggct ggctggcaca
    43021 gcttcctgct aaatatcacc agcgcgccac ctgcatgttt gaccgccacg gtctgctggc
    43081 gctgctggct ggacgttttc ttgcatttgt ccgtacgctg ctgccaacca tggcgggaat
    43141 ttccggtctg ccaaaccgcc gcttccagtt tttcaactgg ttaagtggat tgctgtgggt
    43201 cagcgtggta accagttttg gctatgcctt aagtatgatt ccgttcgtta aacgccatga
    43261 agatcaggta atgacgttcc tgatgatcct gccaattgcc ttgttaaccg ctggcttgtt
    43321 aggcacgctg tttgtggtga ttaaaaaaaa atactgtaac gcctgacgat tttccccgtt
    43381 cccggctgct gtaccgggaa cgtatttaat tcccctgcat cgcccgcatt cttgccgcat
    43441 cttcccccgg cgtcacaccg aagtaacgtt taaactcacg gctaaattgc gatgcgcttt
    43501 catagccgac gcgcatcgct gctgcgctgg ccttcatgcc gtcatggatg atcatcatcc
    43561 gcgccttatg cagacggtaa ttcttcaaat actgcaacgg cgaggtgctg gtgacagact
    43621 taaaattatg gtggaacgcc gatacgctca tgttggcttc tgccgccagt tgctcgacgc
    43681 tcaggttttc ggtgtattta ttctcaattc gtttcagcac gcggctaatc agactgaagt
    43741 gagtctggcg actgaccagc gccagtaacg cgccgccgca aggtccggtc agcacgtagt
    43801 acagaatttc gcggatgatc tgtttgccga gaatacgcgc atccagtggt cgctccatca
    43861 cgtcgagtaa ccgctccgcc gcgaataaaa tctcttctga taacgtggcg gagttaatcc
    43921 cgctggctgc catcgacggc tggaaatgct catcttcgcc aatgtccatc aacagttcct
    43981 gtaactgcaa aatatcgaca ttgagacgca accctgccag cggcacctct gacgtggcat
    44041 aggtttcgca ctcaaacggc aacggcaccg tcagcagcag gtattcattg gcatcataac
    44101 gaaacacgcg
//
LOCUS       DAJTYF010000018        43167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTYF010000018 DAJTYF010000000
VERSION     DAJTYF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.018"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..403
                     /locus_tag="OO590_001134"
     CDS             <1..403
                     /locus_tag="OO590_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6636583.1"
                     /translation="GAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWR
                     PKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQ
                     FSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            428..3027
                     /locus_tag="OO590_001135"
                     /pseudo
     CDS             428..3027
                     /locus_tag="OO590_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3018..4088
                     /locus_tag="OO590_001136"
     CDS             3018..4088
                     /locus_tag="OO590_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6636584.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESSDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQVIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4100..4642
                     /locus_tag="OO590_001137"
     CDS             4100..4642
                     /locus_tag="OO590_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6636585.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGAGVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIQLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4650..5165
                     /locus_tag="OO590_001138"
     CDS             4650..5165
                     /locus_tag="OO590_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6636586.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTLDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5131..5868
                     /locus_tag="OO590_001139"
     CDS             5131..5868
                     /locus_tag="OO590_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6636587.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5979..6989
                     /gene="pyrD"
                     /locus_tag="OO590_001140"
     CDS             5979..6989
                     /gene="pyrD"
                     /locus_tag="OO590_001140"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6636588.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7163..7705
                     /gene="zapC"
                     /locus_tag="OO590_001141"
     CDS             7163..7705
                     /gene="zapC"
                     /locus_tag="OO590_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6636589.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7702..8811)
                     /gene="ycbX"
                     /locus_tag="OO590_001142"
     CDS             complement(7702..8811)
                     /gene="ycbX"
                     /locus_tag="OO590_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6636590.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9055..11163
                     /gene="rlmKL"
                     /locus_tag="OO590_001143"
     CDS             9055..11163
                     /gene="rlmKL"
                     /locus_tag="OO590_001143"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6636591.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11175..13082
                     /locus_tag="OO590_001144"
                     /pseudo
     CDS             11175..13082
                     /locus_tag="OO590_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13212..14465
                     /gene="pqiA"
                     /locus_tag="OO590_001145"
     CDS             13212..14465
                     /gene="pqiA"
                     /locus_tag="OO590_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6636592.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAVVIFLASIMVPTLKMSAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14470..16110
                     /gene="pqiB"
                     /locus_tag="OO590_001146"
     CDS             14470..16110
                     /gene="pqiB"
                     /locus_tag="OO590_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6636593.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16107..16670
                     /gene="pqiC"
                     /locus_tag="OO590_001147"
     CDS             16107..16670
                     /gene="pqiC"
                     /locus_tag="OO590_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6636594.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16926..17093
                     /gene="rmf"
                     /locus_tag="OO590_001148"
     CDS             16926..17093
                     /gene="rmf"
                     /locus_tag="OO590_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6636595.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17163..17681)
                     /gene="fabA"
                     /locus_tag="OO590_001149"
     CDS             complement(17163..17681)
                     /gene="fabA"
                     /locus_tag="OO590_001149"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6636596.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKIT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17750..19510)
                     /locus_tag="OO590_001150"
     CDS             complement(17750..19510)
                     /locus_tag="OO590_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6636597.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19695..20147
                     /gene="matP"
                     /locus_tag="OO590_001151"
     CDS             19695..20147
                     /gene="matP"
                     /locus_tag="OO590_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6636598.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20223..21269)
                     /gene="ompA"
                     /locus_tag="OO590_001152"
     CDS             complement(20223..21269)
                     /gene="ompA"
                     /locus_tag="OO590_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6636599.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21625..22134)
                     /gene="sulA"
                     /locus_tag="OO590_001153"
     CDS             complement(21625..22134)
                     /gene="sulA"
                     /locus_tag="OO590_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6636600.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22353..22982
                     /gene="sxy"
                     /locus_tag="OO590_001154"
     CDS             22353..22982
                     /gene="sxy"
                     /locus_tag="OO590_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6636601.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22945..25107)
                     /gene="yccS"
                     /locus_tag="OO590_001155"
     CDS             complement(22945..25107)
                     /gene="yccS"
                     /locus_tag="OO590_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6636602.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25117..25563)
                     /locus_tag="OO590_001156"
     CDS             complement(25117..25563)
                     /locus_tag="OO590_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6636603.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25686..27740
                     /gene="helD"
                     /locus_tag="OO590_001157"
     CDS             25686..27740
                     /gene="helD"
                     /locus_tag="OO590_001157"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6636604.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSDVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27772..28230)
                     /gene="mgsA"
                     /locus_tag="OO590_001158"
     CDS             complement(27772..28230)
                     /gene="mgsA"
                     /locus_tag="OO590_001158"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6636605.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28326..28988)
                     /locus_tag="OO590_001159"
     CDS             complement(28326..28988)
                     /locus_tag="OO590_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6636606.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29161..29574
                     /locus_tag="OO590_001160"
     CDS             29161..29574
                     /locus_tag="OO590_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6636607.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29619..29936)
                     /gene="hspQ"
                     /locus_tag="OO590_001161"
     CDS             complement(29619..29936)
                     /gene="hspQ"
                     /locus_tag="OO590_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6636608.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(29994..31184)
                     /gene="rlmI"
                     /locus_tag="OO590_001162"
     CDS             complement(29994..31184)
                     /gene="rlmI"
                     /locus_tag="OO590_001162"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6636609.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31279..31557
                     /gene="yccX"
                     /locus_tag="OO590_001163"
     CDS             31279..31557
                     /gene="yccX"
                     /locus_tag="OO590_001163"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6636610.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31554..31883)
                     /gene="tusE"
                     /locus_tag="OO590_001164"
     CDS             complement(31554..31883)
                     /gene="tusE"
                     /locus_tag="OO590_001164"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6636611.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31974..32633)
                     /gene="yccA"
                     /locus_tag="OO590_001165"
     CDS             complement(31974..32633)
                     /gene="yccA"
                     /locus_tag="OO590_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6636612.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32840..32927)
                     /locus_tag="OO590_001166"
     tRNA            complement(32840..32927)
                     /locus_tag="OO590_001166"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32891..32893),aa:Ser,seq:tga)
     gene            33353..34471
                     /gene="hyaA"
                     /locus_tag="OO590_001167"
     CDS             33353..34471
                     /gene="hyaA"
                     /locus_tag="OO590_001167"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6636613.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLTKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34468..36226
                     /gene="hyaB"
                     /locus_tag="OO590_001168"
                     /pseudo
     CDS             34468..36226
                     /gene="hyaB"
                     /locus_tag="OO590_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36245..36952
                     /gene="hyaC"
                     /locus_tag="OO590_001169"
     CDS             36245..36952
                     /gene="hyaC"
                     /locus_tag="OO590_001169"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6636614.1"
                     /translation="MQQKSDNIVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36949..37536
                     /locus_tag="OO590_001170"
     CDS             36949..37536
                     /locus_tag="OO590_001170"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6636615.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37533..37931
                     /gene="hyaE"
                     /locus_tag="OO590_001171"
     CDS             37533..37931
                     /gene="hyaE"
                     /locus_tag="OO590_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6636616.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37928..38785
                     /locus_tag="OO590_001172"
     CDS             37928..38785
                     /locus_tag="OO590_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6636617.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38919..40463
                     /gene="appC"
                     /locus_tag="OO590_001173"
     CDS             38919..40463
                     /gene="appC"
                     /locus_tag="OO590_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6636618.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40475..41611
                     /gene="appB"
                     /locus_tag="OO590_001174"
     CDS             40475..41611
                     /gene="appB"
                     /locus_tag="OO590_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6636619.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41623..41715
                     /gene="cbdX"
                     /locus_tag="OO590_001175"
     CDS             41623..41715
                     /gene="cbdX"
                     /locus_tag="OO590_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6636620.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41795..43093
                     /gene="appA"
                     /locus_tag="OO590_001176"
     CDS             41795..43093
                     /gene="appA"
                     /locus_tag="OO590_001176"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6636621.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
BASE COUNT        10743 a        10502 c        11498 g        10424 t
ORIGIN      
        1 tggtgcgccg ttggcgaaag acagagaaac ccttttcttc actagcgtac gggcagtccc
       61 ttcaacaacg aagcgggaag agggaaatac cctgaagatt gccacacaaa gcgtcatcaa
      121 acttttctgg cgaccaaaag gtttagcgta tcccttaggc gaggctccgg cgaaactgcg
      181 ttgcacttcg tcagctgaca tggttacggt cagtaaccca acaccttatt tcattaccct
      241 gacagacctg aaaataggtg gaaaagtagt taaaaatcaa atgatttccc cctttgataa
      301 ataccaattc tctctgccaa agggggccaa aaatagcagc gtaacgtatc gaaccatcaa
      361 tgactacggg gcggaaacgc cgcaactcaa ctgtaaatcg taagccgtct tcagttaaga
      421 gagcgagatg tatagaactc atcgacaaca cagcctgtta agctctggtg gagtgccatc
      481 gtttattggt gggctggtgg tgtttgtgtc ggcagcgttc aatgcacaag ctgaaacctg
      541 gttcgatcct gcttttttca aagatgatcc ctcaatggtg gccgatttgt ctcgtttcga
      601 aaaaggacaa aaaataacgc caggggttta tcgtgtcgat attgttctga atcagacaat
      661 tgtagatacg cgcaacgtca attttgttga gataacgcca gagaagggga ttgccgcctg
      721 tttgacgact gaaagcctgg atgcaatggg tgttaatact gatgcgtttc cggcttttaa
      781 acaactggac aaacaagcgt gtgtgccatt ggcggagatt attccggatg ccagcgtaac
      841 gtttaatgtg aataaactcc gtctggaaat ttcagtaccg caaatcgcca tcaaaagtaa
      901 tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg atcaacgcgc tattactggg
      961 atattcgttt agcggggcta acagtattca tagcagcgca gacagtgatt ctggcgacag
     1021 ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc gcaacaattc
     1081 aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca gctatttgca
     1141 gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata ctgcaggcga
     1201 ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca acatgctgcc
     1261 agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca atgcccagat
     1321 aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg gtgcttttga
     1381 aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa tcaaagaagc
     1441 ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac tccagcgtca
     1501 ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta atgaacagca
     1561 ggagagcaaa tttgcccagg ccacgttgca gtggggcgga ccgtggggaa cgacatggta
     1621 tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg gatttaacct
     1681 tggtgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc tggcagagca
     1741 aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca accaattggg
     1801 cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca ccctttccga
     1861 caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg aagatacgcc
     1921 gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc aggtcaatat
     1981 ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc aaacttactg
     2041 gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa ttaaagatct
     2101 ctcgctgggg gtttcctgga actacagtaa gtcccgtggt caacctgatg ctgatcaggt
     2161 gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta atgatagcta
     2221 taccaggaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa cgaagggcac
     2281 attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct gagctacagc
     2341 gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag catggactac
     2401 aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg cagtcaacaa
     2461 caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat taccctgggg
     2521 caatcgctgg gggaaactaa cgtgctgatt gtagcaccag gcgcagagaa tactcgtgtg
     2581 gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc ctatgccact
     2641 tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa cgtggatctt
     2701 gaaaatgcag tagtcaacgt ggttcccacc aaaggggcgt tggttctggc ggagttcaat
     2761 gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc gctgcgtttt
     2821 ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga tgatgatggc
     2881 tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg gggcgaagct
     2941 cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa ctctgcgatt
     3001 acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta ttactacgac
     3061 tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat atcgggcagt
     3121 gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa actctggatg
     3181 aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac gaatcgagtg
     3241 attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga tgggcattcg
     3301 ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat gactatcttg
     3361 ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct ttcgaaaacg
     3421 tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa cgtttaggtg
     3481 gccagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag ccgttcgttg
     3541 gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac aacatacccg
     3601 caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg tctggtacga
     3661 tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt gatctgggcg
     3721 acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc agaacggcag
     3781 aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac gtcaatctga
     3841 gtctgacggc aacctcagac cccagctatc cccaggtgat taagacgtca cgtcctggcg
     3901 tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt gggacactac
     3961 cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat ccagtcagca
     4021 cgacaggcgt accaccagaa accgggcgat ttgaagccac ggcaacggtg agaataaatt
     4081 ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat cagtgcgtta ttttgttctt
     4141 tagccttctt aacatccatg tcgggatggc tgggcctgaa caagtcagta tgcatattta
     4201 tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac aaaatattca
     4261 tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca ctgctgctga
     4321 tttgaatatt gatatcaccg gttgtgccgc tggtattact ggcgcgggcg tcctttttag
     4381 cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag gcggaagcgg
     4441 tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc aagaaatcca
     4501 gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac tcaaatatca
     4561 acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg cggttctcta
     4621 ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg attctgttct
     4681 tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc aacattagcg
     4741 gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc aatgtgcagt
     4801 taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag aaatctttca
     4861 tcattaattt gcaggattgc ggaacagatg tcagcacggt tgatgtcacc ttttcaggaa
     4921 ccctcgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat gcagccagtg
     4981 ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac caggcgagta
     5041 aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa ttgcgacctg
     5101 tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta cttcattatg
     5161 actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct gctggtggcg
     5221 ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc ggcagacaga
     5281 gaatcgatat ctattttact gactaatacc agtcaggaat cttggcttat taacagcaaa
     5341 atcaatcgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc actattactg
     5401 gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg cttgctgaga
     5461 acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag cattgccagc
     5521 gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc ggtatttaaa
     5581 ctgttctggc gacacgaagg tttgccgggc gacccgctgg aagcttatca acaattacgc
     5641 tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta cattaacctg
     5701 attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc gcctaaaagc
     5761 cagcgtcaga caaactggtg tcaggtaata gcaccgtgtc atgtcgcctg gcggacgata
     5821 aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa caggttctga
     5881 aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccgaact cgcacataat
     5941 acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc ttcgttcgta
     6001 aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag caattacgcc
     6061 gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg aaacctgtta
     6121 actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt gataaagacg
     6181 gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc ggtaccgtca
     6241 cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta gatgccgaag
     6301 gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta gagaacgtaa
     6361 aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa gatacgccag
     6421 tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc tatgcgggat
     6481 atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg caatatggtg
     6541 aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg caagcgatgc
     6601 accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa gacgaattga
     6661 tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca accaatacca
     6721 ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt ggcttaagtg
     6781 gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta gaattaaacg
     6841 gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg cgtgaaaaga
     6901 ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa ggtccgccgc
     6961 tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt tataagataa
     7021 tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt atttagacat
     7081 tttgtacttt tattgttgag gttattaagc gaagcaacaa tggattgtgt tgctgcggtt
     7141 ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc gttggtatta
     7201 cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat ttcgctcacg
     7261 ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt gcgttgatga
     7321 tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat ctaaagacca
     7381 aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac cgcaaatgcc
     7441 gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg gcgatgcggc
     7501 ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg aatctggcga
     7561 aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc gcgcgatgca
     7621 gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga atgttgacag
     7681 cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg gtacacagct
     7741 acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg tgacttcgcc
     7801 ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat aagggatacg
     7861 aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac gaaatgcctg
     7921 tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg cggtatcatc
     7981 agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca cctcatcgcc
     8041 aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga catcgccgtt
     8101 atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg gatgtttttg
     8161 ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa ccacatcaaa
     8221 caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg acgcgccgga
     8281 aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac gttgttggag
     8341 atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa atgacagagg
     8401 tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc gtaattgcac
     8461 ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa ttcgcgcggt
     8521 aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag caaaacgaac
     8581 atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag gtgaaggagt
     8641 aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag gttccgtgat
     8701 cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg taagaccaat
     8761 gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca cggtgcggtc
     8821 ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct ataatgcgca
     8881 acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg ccctgggttg
     8941 tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc catgtccaga
     9001 ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga cgatatgaat
     9061 tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga actggaaaat
     9121 ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg cgacacacgg
     9181 cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt gccgctgggc
     9241 gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat caactggaca
     9301 gagatgttta atcctggcgc gaccttcgct gtccacttca gtggtttgaa tgacaccatc
     9361 cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc tttcacgcgg
     9421 aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt taacgtctgg
     9481 ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt acatctgcgt
     9541 ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc cgctattgtg
     9601 atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg ttccggtacg
     9661 ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca ccgtgggcgt
     9721 tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt gaaagcggaa
     9781 gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta cggttcggac
     9841 agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg gattggtgaa
     9901 ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc gaaagggccg
     9961 tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga accggcgctg
    10021 attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg ctggaatctc
    10081 tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc agacaaacag
    10141 tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt tgccgaaagc
    10201 acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct gcgtaagaac
    10261 ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg cctgtatgac
    10321 gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt ggtggtgcag
    10381 gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct gttcgatatt
    10441 atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct gaaaacccgt
    10501 gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga gtttcttgaa
    10561 gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga tactggtctg
    10621 ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg caaagatttc
    10681 ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg cggtgcacgc
    10741 agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg caacctgcgt
    10801 ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct ggcgtggctg
    10861 cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt ctctaactca
    10921 aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat gaaagatttg
    10981 aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg tggcttccgt
    11041 atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac gcaaaaaacg
    11101 ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat taccgcagcc
    11161 tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg ttcagcgacg
    11221 cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt tgtctggtgg
    11281 gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa caagggctgg
    11341 atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa caggatccgc
    11401 cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa gaacaagcgg
    11461 aatatctgaa acgctatcac gatatttcgt gcctggtgat gaacgacccg agcgagaaaa
    11521 atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg tggcagctgg
    11581 aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt gcgctgtcgt
    11641 cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg agcaatccgc
    11701 gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc gactggctgg
    11761 aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac cgttcgttta
    11821 tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg acctatccag
    11881 ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa gaactacaaa
    11941 atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgctag gggatcaaag
    12001 cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt cgcgaacgtg
    12061 gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc agccgctccg
    12121 gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag caactggtga
    12181 aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt ccgaatgggt
    12241 gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcgggc agcgggcgta
    12301 ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg gaactggatc
    12361 ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg gttaatggca
    12421 agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa cgggcgatga
    12481 cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg cgtttgttcc
    12541 tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat gtcgaaacgc
    12601 tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg gttagccacg
    12661 atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc ggcggtaaaa
    12721 ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa tatgtggcac
    12781 tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca gaaactgtaa
    12841 aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag ctaccgcaat
    12901 tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg gatgcttcct
    12961 tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct gctgcagagc
    13021 aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa aatggtggct
    13081 gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg cctgatgaac
    13141 tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata gcgcatcatt
    13201 aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc tcgcagtgtg
    13261 acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt ccccggtgtg
    13321 gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc tatgcgttgg
    13381 ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg aacgttgcag
    13441 gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct gaggactacg
    13501 ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt tgtctgataa
    13561 ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag caactggcac
    13621 gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg ggtgtgctgg
    13681 tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc tttctcccct
    13741 ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt cgctggttat
    13801 gggacgacat cgccccgatg ccagaactgc gccagccgct aaaaccaggc gtcacgggga
    13861 tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct gatgaacccg
    13921 tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg cagtggacac
    13981 tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg cccatcatgg
    14041 tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc attctgttat
    14101 ggagcgaagg ctcttatccc gtcgctgtgg tgatctttct ggccagtatt atggtgccaa
    14161 cgttaaagat gagcgccatc gcgtggctgt gttgggatgc caaagggcat ggcaagcgcg
    14221 acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc tggtcgatga
    14281 ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt ttaatgagta
    14341 tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca atgttttctg
    14401 ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag catgaggagt
    14461 cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa gaactggtct
    14521 cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct tttttatcat
    14581 tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg aattgaaggt
    14641 ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag cgccacactg
    14701 gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat ggaaaaattg
    14761 ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga agggattagc
    14821 ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc gaaaggcagc
    14881 aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga tgcgaaaggt
    14941 atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga tccggtgctg
    15001 ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca aaaacgcaat
    15061 atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag caatgttcgc
    15121 ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg tgtggagatg
    15181 ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga aggtctggat
    15241 ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca gaagagcatt
    15301 caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga ttcggtacgc
    15361 ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac cgtaagcaaa
    15421 gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg tattccggta
    15481 ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc ggatgttgtt
    15541 gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac cggaaacctg
    15601 gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc aataaccggt
    15661 attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct ggcgcaaatc
    15721 cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa tccgatgatt
    15781 gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct gcaaacgacg
    15841 ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc gacggatatg
    15901 cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg ctccgcagcc
    15961 tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga actgcaaccg
    16021 gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa ggacaaaaaa
    16081 gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt gcagcactgt
    16141 ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta cctgtggtgc
    16201 agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta gagcaggtca
    16261 ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat gtgaagtatg
    16321 tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc aacaccctgg
    16381 ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct ctgggaagcg
    16441 cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat ggcaaggtca
    16501 ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt ccgttccgtc
    16561 tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg gccggtgtct
    16621 ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa ttataaagat
    16681 ttgtaaatat aaccgtctcc ggtatgccgc ctgaggcggt ttttttgttt ctaacgtgcg
    16741 gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta gctcacaaat
    16801 atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta ttgcttaact
    16861 gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta tgagggaaac
    16921 gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt ggttatcagg
    16981 ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat caaaggtcac
    17041 aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc tgattctgtc
    17101 tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct gtctgatgat
    17161 aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc ggtatagatc
    17221 agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg acgattaaca
    17281 atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac ctgaccagtg
    17341 aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag ccagccgagg
    17401 tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat aaccggatcg
    17461 ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc tgcttcaaca
    17521 taccctttgt cgaagttacc acccgtttcg gttattttga ccacacggtc catcatcagc
    17581 atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg accagaggca
    17641 agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt aagccttatt
    17701 ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat cagttcggaa
    17761 taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc tgttgcgatg
    17821 cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg ccatcccaca
    17881 ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag atggtgaatt
    17941 tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga acgttagcag
    18001 tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata gcaaagaagc
    18061 cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga tcgactgaac
    18121 ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc agggcgcaga
    18181 gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca aatgtcagcg
    18241 atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac gcctgcatga
    18301 tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg atgtcggtga
    18361 attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca aaagcgcgtg
    18421 gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt tcgccttcgg
    18481 tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg aggaaacctt
    18541 cgcgccattc acgctgctgc agcattaaat ttagctgctc gccggagaag gtgtcgccat
    18601 cacacaggga ggcgacctct ttacactggc ggaggatcca atgcgggcta agcggaagtg
    18661 tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa gcatccgctc
    18721 ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac cactggctta
    18781 ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata gcctgctctg
    18841 aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct accagaatga
    18901 ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac tcatcaaacg
    18961 caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac agcagaggtt
    19021 gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga tgcaccagac
    19081 caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt tgctccgctt
    19141 ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct gcactcactg
    19201 cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc agttggcctg
    19261 catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca gactcttccg
    19321 gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc tccagcgcaa
    19381 attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc aaatgtggct
    19441 gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat gcaagtttcg
    19501 taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata ccgtaaccgg
    19561 cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa agctcacagg
    19621 atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag ttacacggta
    19681 acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt tggaaatgga
    19741 agtatctggt aaagaagcat cgcgaagggg agttaattac ccgttacata gaagccagtg
    19801 ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg ctggtaaatg
    19861 gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag accattcggg
    19921 ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa tcgatcgatc
    19981 tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt aaaacgcttt
    20041 ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac gcgaataaaa
    20101 tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg acgaaagtca
    20161 gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta ccagacgata
    20221 acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat ctctacgcga
    20281 cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc acaggtgttg
    20341 ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc cgggatacct
    20401 ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc ctggttgtaa
    20461 gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc tttcggatcc
    20521 aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg tttcagggtt
    20581 gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt ctgtacttcc
    20641 ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg gtaggaaaca
    20701 cccaagctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc accgatgttg
    20761 ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta ctcaacacca
    20821 cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc accaggtacg
    20881 ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat gtccagatcg
    20941 tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt gtatgcgccg
    21001 ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat ttcaaaacca
    21061 acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt ttcgtgggtc
    21121 ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag tttagcacca
    21181 gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc agccagtgcc
    21241 actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc caaaatacgc
    21301 catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc tgctcaatat
    21361 taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa gtctacaacg
    21421 tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt cttgtataag
    21481 gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa accgcttcag
    21541 acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg acaaaaaaag
    21601 ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt ttagcccgga
    21661 aagttgtctc gttgtgtgag aggatgcgct taccggacgc ataataaacc ccatagcgtt
    21721 accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat catctgtcaa
    21781 ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg actccactgt
    21841 gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc cagatgcctg
    21901 aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc gcgattgctg
    21961 accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct gatcttcgcg
    22021 atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac gcgcaatttt
    22081 acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt acataatcaa
    22141 tccagcccct gtgagttact gtatggatgt gcagtacatc cagtgacaac aaagatcaac
    22201 cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac ataaattaat
    22261 cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta atgtgaatca
    22321 ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat aagcggatct
    22381 ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca ttgtttggca
    22441 gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt gagttgtatc
    22501 ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc tggctgacat
    22561 ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa agtctatggc
    22621 gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg ctgaaagaga
    22681 aaagcacgcg caatacccgg gaaagactga aagatttgcc caatatgtct tttcatctgg
    22741 aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata cttggggcaa
    22801 aaatgtgttg gttgcgactg cggcagcaaa acagtctggt gacagaaaag attctgttta
    22861 tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca cgccgccagg
    22921 agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg gagcttgagt
    22981 aatctggcgt tgcagacgac caatctctgg cagtaatgca atcaataacc caacttgttg
    23041 tacgaccaga ggttctttgc tatctgcccg tggttcaagt tgctgcatcc gctgtttcag
    23101 gccagctaat gcctgattga cgcgttcttc atcagcaggt tgatgatgta acgcgtcatc
    23161 aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta actgctcccg
    23221 gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca gcaagcgaaa
    23281 cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg ataccacaga
    23341 cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg ccagacggtt
    23401 atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt tggcctctgt
    23461 ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcgggccaga tgtagctcac
    23521 tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta acgctacttc
    23581 aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga acatcgttgc
    23641 atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa ccagcagcac
    23701 cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg cgatacctac
    23761 cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt ttggctggca
    23821 gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta tttgaatgat
    23881 ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga gggcggattc
    23941 cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg ggctgtcatc
    24001 agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg attcaattgt
    24061 tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg ttttgagtaa
    24121 atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat gcgtaaaagc
    24181 gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc gtgacagttg
    24241 ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca cgtcgctgtg
    24301 gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac gctcgtgaat
    24361 atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac cacgatcgcc
    24421 acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca gctgaccgtt
    24481 ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag gatcaaacat
    24541 gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca ggttgtcctg
    24601 cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt accagacggc
    24661 accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg ttcccaacat
    24721 agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt gacccagacc
    24781 gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata gccagggcca
    24841 gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg taatgatgag
    24901 gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg ccaccatccc
    24961 cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg ctgtggttcc
    25021 acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccaag tatagcgttt
    25081 gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac gacgtgcatt
    25141 ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa ccggccatag
    25201 tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga ttgaaataca
    25261 ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga aaatcagcca
    25321 gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag ccgggttcag
    25381 ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag tgatctccca
    25441 gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag tcgccaacag
    25501 ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca aaacggtacg
    25561 cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac gtttttttgg
    25621 gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg tggatctaca
    25681 gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac ccttacgatc
    25741 gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa taccttattc
    25801 ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc gagctggaat
    25861 ttgtactgcc ggacgaaaaa gtggtgcgtc tgcacggcac agaatggggc gagacgcagc
    25921 gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg agcgaaattg
    25981 cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga gaaaataaat
    26041 ggctgacgcg tgagcaaacc tctgacgttc agcaacaaat ccgccaggct ttgtcggcgt
    26101 tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg tggcgtaaat
    26161 gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag gcgtataccg
    26221 aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca ccgctgaatc
    26281 cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg gccggtgcag
    26341 gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg cgtggtgaag
    26401 cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa gagatggacg
    26461 agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt catgcgctgg
    26521 cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa ctggaaaatg
    26581 ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc agcgaaaaga
    26641 aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg tcagtgccag
    26701 aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc ctcgatcgct
    26761 gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc agtgcacccg
    26821 aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta ttaaaagcct
    26881 ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt catcaggcga
    26941 ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg gttgatgaat
    27001 ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag caaaacagtc
    27061 agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc agcggtgcgc
    27121 aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc tgtgatttag
    27181 acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt attcagcaga
    27241 acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa aaagctgtca
    27301 cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt tatgccaaac
    27361 cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc agcctggaaa
    27421 aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat gccagcaaag
    27481 ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtgat ggttttccgg
    27541 ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag gatttcccgg
    27601 acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat cgggtatggg
    27661 cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat ctggatgtgc
    27721 cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat attacttcag
    27781 acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt cgttgaaatg
    27841 cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa tgttccatac
    27901 cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat ttagtggatc
    27961 ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa cctgctggtc
    28021 accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc gggaaatcaa
    28081 gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt gatgccgttc
    28141 cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa tatgtttccg
    28201 cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac tttcctacag
    28261 attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc acaggcacaa
    28321 aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg cgtttgtggg
    28381 tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga ctgcggtgga
    28441 atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat catcgtggca
    28501 aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc gacctcataa
    28561 tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg aatcggcgtc
    28621 gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc ttcccgctca
    28681 ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt aaaactgacc
    28741 accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat cgtcttctca
    28801 acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc accgcgcagc
    28861 agagagctgg aaaccttttt gccgtcgagt accagaagat cgacatcggt tgaaagccgc
    28921 agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt cacgatgccg
    28981 gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca aaatttttct
    29041 ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt cattcgtatt
    29101 agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct ggaggaaata
    29161 atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc gctggtgggg
    29221 gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact cgaccagggg
    29281 tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg ccagcagggg
    29341 tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt ccgtaattca
    29401 gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac cctctggatg
    29461 caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg gttaaatgtg
    29521 gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa ataaaaaatc
    29581 cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag acgcggggcc
    29641 tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc aggatgttca
    29701 tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg taggccgtta
    29761 tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt caccgccagc
    29821 tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac cactccgaga
    29881 taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc aatcatagtc
    29941 acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc aagttacatg
    30001 acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat caccggatga
    30061 tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc aattgcggca
    30121 tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga gaaagtcagg
    30181 agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt ataaccacga
    30241 cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat cacgataacg
    30301 tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc atcacggaca
    30361 aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc aatatccagc
    30421 gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag tgccgatacg
    30481 gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac gtagcggcgg
    30541 gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc gtgctgaata
    30601 tccaccagca gcttcatgcc gtgttcttca atcggcagca gggcaggcgg caactcgccg
    30661 gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac atcgctgcga
    30721 tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc cgcgcgctga
    30781 tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg atcgatagta
    30841 atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag gccatctttt
    30901 tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa aaaagcaatg
    30961 tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga agctggcgaa
    31021 taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt ttcaccgagg
    31081 ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg gcgacgaagt
    31141 aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta cttttctgtc
    31201 gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac gcgtccatta
    31261 aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta cgggcgagtt
    31321 cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg cttaaccggg
    31381 tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga agaagggcag
    31441 gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg tgtagaacgg
    31501 gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat ccgttaaata
    31561 cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg gcctttcggg
    31621 aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt cgccatcgct
    31681 tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc acgtacaaaa
    31741 cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc aatcaccact
    31801 gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc cgtttctatc
    31861 tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc ggcagtgtaa
    31921 caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg agattaatcg
    31981 cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag cgaaacatac
    32041 aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt gctggtttca
    32101 aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag atgcagagca
    32161 ggcagttgca ggaagatatt agcaaccata ccaatcagta ccaccacaat acccgccatc
    32221 agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc agagcagcag
    32281 aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat tccggcagac
    32341 agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa ggcgaatgcg
    32401 gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa accatacata
    32461 cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact ggcagttgcg
    32521 gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg cagcacctta
    32581 tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc cattgagtca
    32641 ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag ggcacccaga
    32701 agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat tatcaggtca
    32761 ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg ttagcattta
    32821 gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa ccctttcggg
    32881 tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga atgacgcgca
    32941 ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct gaaaaacagc
    33001 caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac catgaattcc
    33061 ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta aagaaaaata
    33121 ggtccatttt tatcgctaaa agataaatcc acacagtttg tgttgttttg tgcaaaagtt
    33181 tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa ggatgagctt
    33241 tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg tcgcttgccg
    33301 accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg atatgaataa
    33361 cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca gctttctcaa
    33421 atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac caaagattgc
    33481 ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc tggaatgtac
    33541 ctgctgtacc gaatctttta tccgctccgc tcacccactg acgaaggacg tcatcctttc
    33601 cctgatttcc ctctattacg acgataccct gatggctgcc gccggaaccc aggcggaaga
    33661 agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag tagaaggtaa
    33721 tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt ttattgagaa
    33781 actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct gcgcgtcctg
    33841 gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg acaaagtcat
    33901 caccgacaaa cccattatca aagtacctag ctgcccgccg atcccggatg tgatgagcgc
    33961 catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa tgggtcgtcc
    34021 actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc acttcgacgc
    34081 cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt gcctgtacaa
    34141 aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct ggaatgatgg
    34201 cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa atggtttctg
    34261 ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta ctcattccac
    34321 cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg gtgtgcacgc
    34381 agttgccagc gccgttgacc aacgcagacg tcataaccag caacctacag aaaccgaaca
    34441 tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca gggatacacc
    34501 atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga aggccacatg
    34561 cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc ctgcggcacc
    34621 atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc gtgggcgttc
    34681 gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt ttacgccatc
    34741 gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa cattatgctg
    34801 acaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg gatggactgg
    34861 atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact ggcgcaaagt
    34921 ctctcctctt ggccgaaatc atcccctagc tatttcttcg acgtacaaaa tcgcctgaag
    34981 aaattcgttg aaggcgggca gttggggatc ttccgcaatg gctactgggg gcacccgcag
    35041 tacaaattgc cgctagaagc caacctgatg ggctttgccc actatctcga agctctcgat
    35101 ttccaacgtg aaattgtcaa aattcacgcg gtctttggcg gtaaaaaccc gcatccaaac
    35161 tggattgtcg gcgggatgcc ttgcgccatt aacattgacg aaagcggcgc ggtcggggcg
    35221 gtcaatatgg aacgcctgaa cctggtgcag tcgattatca cccgcacggc agacttcatt
    35281 aacaacgtga tgatccccga cgccttagcc atcggtcagt tcaacaaacc gtggagcgaa
    35341 atcggcacgg gtctttctga taaatgtgtc ctcagctacg gcgcattccc ggatattgcc
    35401 aacgactttg gtgagaaaag tctgctgatg cctggcggcg cggtgattaa cggcgacttc
    35461 aacaatgtgc tgccagtgga tttggttgat ccgcagcagg tgcaggagtt tgtcgatcac
    35521 gcctggtatc gttatcccaa cgatcaggtc gggcgtcatc cattcgatgg cattaccgac
    35581 ccgtggtaca accccggcga tgtcaaaggc agcgatacca acattcagca gctgaatgaa
    35641 caggaacgct actcgtggat caaagcgcca cgctggcgcg gtaacgcgat ggaagtgggg
    35701 ccgctggcac gcacgttaat cgcttatcac aaaggcgatg ctgcgaccgt tgagtcggtc
    35761 gatcgcatga tgtcggcgtt gaacctgccg ctttccggta tccagtcaac gttgggccgc
    35821 aggtaagttg cagtatttct tcgacaagct gatgaccaac ctgaaaaatg gcaatctcgc
    35881 cactgcttcc actgaaaaat gggaacctgc aacctggccg acagagtgcc gtggcgtcgg
    35941 ctttaccgaa gcgccgcgcg gagcgttagg ccactgggcc gccattcgcg atggcaagat
    36001 tgatctctac cagtgcgtgg tgccgaccac ctggaacgcc agcccgcgcg atcccaaagg
    36061 gcagattggc gcttatgaag cggcgctgat gaacaccaaa atggcgatcc ccgagcaacc
    36121 gctggagatc ctgcgtactc tgcacagctt tgatccgtgc ctcgcctgtt caactcatgt
    36181 gctgggcgac gacggtagcg agctgatctc cgtgcaggtg cgttaacagc gaaggagaat
    36241 catcatgcaa cagaaaagcg acaacattgt cagccactat gtctttgaag caccagttcg
    36301 tatctggcac tggctgacgg tgttatgcat ggcggtgttg atggtcaccg gttactttat
    36361 cggcaagccg ctaccttccg tcagtggcga ggcgacgtat ctgttctata tgggttacat
    36421 caggttaatt cacttcagcg ccgggatgat ttttaccgtg gtcttgctga tgcggatcta
    36481 ctgggctttt gttggcaatc gttactcccg cgagctgttt atcgtgccgg tatggcgtaa
    36541 aagctggtgg cagggcgtgt ggtatgaaat ccgctggtat ctgtttctgg caaaacgtcc
    36601 gagtgccgat ataggccata accccatcgc ccaggcggcg atgttcggct atttcctgat
    36661 gtcggtcttt atgatcatca ccggttttgc gctgtacagc gaacacagcc agtacgctat
    36721 ttttgcgccg ttccgttatg tggtggaatt tttctactgg acgggtggca actcaatgga
    36781 cattcacagc tggcatcggc tggggatgtg gctgattggg gcgtttgtga tcggtcatgt
    36841 ctacatggcg ctgcgtgaag acatcatgtc cgacgacacg gtgatctcca ccatggtcaa
    36901 cggctaccgt agccacaaat ttggcaaaat aagtaacaag gagcgttcat gagcgagcaa
    36961 cgcgtggtgg tcatggggct gggcaacctg ctgtgggccg atgaaggctt cggcgtgcgg
    37021 gtggcggaac ggctgtatgc ccattaccac tggcccgagt atgtggagat tgtcgatggc
    37081 ggtactcagg gactgaactt gctggggtat gtcgaaagcg ccagccatct gttgattctc
    37141 gatgccattg actacgggct ggaacctgga aggctgcgaa cctatgccgg agaacgcatt
    37201 ccggcctatc tcagcgcgaa gaaaatgagc ctgcatcaga acagtttctc cgaagtgctg
    37261 gcgctggcag atatccgcgg acatctgcca gcacatattg ccctcgtcgg gctgcaaccc
    37321 gcgatgctcg acgactacgg cggcagcctg agcgaactgg cacgggagca actgcccgct
    37381 gcggaacaat cggcgctggc gcagttggct gcgtggggaa gtgtgccgca accggctaat
    37441 gaatcgcgtt gtctcaatta tgactgtctg tcgatggaaa attacgaagg cgttcgcttg
    37501 cgccagtacc ggatgacaca ggaggagcag ggatgagcaa cgacacgcca tttgatgcgt
    37561 tgtggcaacg aatgctggcg cgcggctgga cgccggtcag tgaatcccgt cttgacgact
    37621 ggcttacgca agcgccagac ggcgtggtgt tattaagcag tgacccgaaa cgcacgccag
    37681 aggtcagcga taatccggta atgattggcg aattactgca cgagtttccc gactatacat
    37741 ggcaggtggc gattgctgac cttgagcaga gcgaagccat cggcgatcgc tttggcgcct
    37801 ttcgctttcc tgccacttta gtgtttaccg gcggaaacta tcgcggcgtg ctgaatggta
    37861 ttcacccgtg ggcggaactg ataaacctga tgcgcgggct tgtcgaaccg cagcaggagc
    37921 gtgcctcatg agcgaaactt ttttccatct gctggggcca ggaacgcaac cgaacgatga
    37981 cagtttcagc atgaatccac tgccgatcac ctgtcaggtg aatgatgaac cgagtatggc
    38041 ggccttggag caatgtgctc acagcccgca ggtgattgcg ctgttaaacg agttacaaca
    38101 tcaactaagc gaacgccagc cgccgttggg cgaggtgctg gcagtcgatc tgttaaatct
    38161 caacgccgac gatcgtcact ttatcaatac gcttctcggg gaaggggaag tgtcagtgcg
    38221 cattcagcag gctgacgaca gtaaaagtga aatacaggag gcgatcttct gcggattatg
    38281 gcgggtgcgc agacgtcgcg gcgaaaagtt gctggaggac aaactggagg ctggctgcgc
    38341 gccgctggcg ttgtggcagg cggcaacgca aaatctcttg ccgacagatt cgctgttacc
    38401 gccgcccatt gatggcctga tgaatggcct accgttggcg catgagttac tggcacatgt
    38461 acgtaacccc gacgcgcagc cgcacagcat taatctgacg caattaccca tcagcgaggc
    38521 tgatcggctt tttctctcac gtctctgtgg gccgggaaat attcagattc gtaccattgg
    38581 ctatggcgag agctatatca actccacggg gttacgccat gtctggcatt tacgctgtac
    38641 ggacacctta aaaggcccgt tactggaaag ttatgaaatc tgcccaatac cggaagtggt
    38701 gctggcagcg ccagaagatt tggtcgactc tgcgcagcgg cttagcgagg tatgtcagtg
    38761 gctggcggaa gctgcaccga cgtaaaaaga cggtaagtat cgctttcagt cttatgaata
    38821 tcgcaatcgg cgaatacctc tggtcgtaga gtttcaggat aaagagggag atctaacatt
    38881 atcgggttat ttttctctct tcgcctacag gagtgcgcat gtgggatgtc attgatttat
    38941 cgcgctggca gtttgctctg accgcgctgt atcacttttt atttgtaccc cttaccctgg
    39001 ggctgatttt tttgctggct atcatggaaa ccatttacgt ggtcaccggc aaaacaatct
    39061 accgcgatat gacgcgcttc tggggtaagc tcttcggtat caattttgct cttggcgtgg
    39121 ctaccggcct gaccatggag tttcagtttg gtactaactg gtcattctat tccaactatg
    39181 tgggcgatat ttttggcgca ccactggcga tggaagcatt aatggccttc ttcctcgaat
    39241 ccacctttgt cgggctgttc ttcttcggct ggcaacggct gaataaatac cagcacctgc
    39301 tggtgacgtg gctggtggcg ttcggttcaa atctctctgc attgtggata ttgaatgcca
    39361 acggttggat gcaatacccg accggtgcgc attttgatat cgacaccctg cgtatggaga
    39421 tgaccagctt cagcgagctg gtctttaacc cggtcagcca ggtgaaattt gtgcacaccg
    39481 tcatggcggg ctacgtgacc ggggccatgt ttattatggc gatcagcgcc tggtatttac
    39541 tgcgcggacg ggagcgcgat gtcgcattac gctcgtttgc catcggttcc gtcttcggta
    39601 ctctggcgat tatcggtacc ctgcaactcg gagacagttc tgcgtatgaa gtcgcgcaag
    39661 tacaaccggt aaaactggcg gcgatggaag gggagtggca aacggaacct gctcctgcac
    39721 cgttccatgt ggttgcctgg ccggaacagg atcaagagcg taacgccttt gccatcaaaa
    39781 ttcccgcgct gctagggatc ctcgccactc actcattaga taaacccgtg ccgggtctga
    39841 agaatttgat ggctgaaacc tacccacgct tgcaacgcgg acgtatggcc tggctgttaa
    39901 tgcaggaaat atctcaaggc aatcgtgagc cgcatgtgtt gcaggcattc cgggagctgg
    39961 aaggcgacct tggttacggc atgttgctct cccgctatgc gccggatatg aatcatgtca
    40021 cagccgcaca gtaccaggcg gcgatgcgtg gcgcgatacc tcaggttgcg ccggtattct
    40081 ggagtttccg catcatggtg ggctgtggtt ccctgctgct actggtgatg ctgattgcgc
    40141 ttgtccagac gctgcgtggc aaaatcgacc agcatcgctg ggtgctgaaa atggcgctct
    40201 ggagtttgcc gttgccgtgg attgcgattg aagccgggtg gtttatgacc gagtttggtc
    40261 gtcagccgtg ggcgatacag gacatcttac cgacatactc cgcgcactcc gctttaacca
    40321 caggacaact ggctttctca ctgatcatga tcgtaggtct ttacaccctg ttcttaatcg
    40381 ccgaagtcta cctgatgcag aaatatgccc gtctggggcc gagcgcgatg cagagtgaac
    40441 aaccgacgca gcaacagggg taaaggagaa aatcatgttt gattatgaaa cattgcgctt
    40501 catctggtgg ctgctgattg gcgtgatcct ggtggtcttt atgatctccg acggatttga
    40561 catggggatc ggctgtttgc tgccgctggt ggcgcgtaat gatgatgaac gccggatagt
    40621 gattaatagc gttggtgcgc actgggaagg caatcaggtc tggttgatcc tcgcaggtgg
    40681 ggcattattt gccgcctggc ccagagtgta tgcagcggcg ttttccggct tttatgtggc
    40741 gatgatcctg gtgctgtgct cgctgttctt ccgtccgctg gcctttgatt atcgcggaaa
    40801 aattgccgat gcacgctggc gtaaaatgtg ggatgccggt ctggtcatcg gcagtctggt
    40861 gccgccggta gtcttcggta tcgccttcgg caacttgttg ctcggcgtgc cgtttgcctt
    40921 cacaccgcaa ttacgcgtgg agtatctcgg cagcttctgg caactgctga cgccattccc
    40981 tttattgtgt ggtttgctca gccttgggat ggtgattttg caaggtggcg tctggttaca
    41041 actgaaaact gttggtgtga ttcatctgcg ttcacagctg gcgactaaac gcgctgcact
    41101 gttggtgatg ctgtgctttt tgctggcagg ttactggctg tgggtcggta ttgatggctt
    41161 tgtactgctc gcccaggatg ctaacggtcc ttccaatccg ttaatgaaac tggtggcagt
    41221 gctacctggt gcctggatga ataatttcgt cgaatcgccc gttttgtgga tcttcccgct
    41281 gctggggttc ttctgcccat tgctgacggt gatggcgatt tatcgtggtc gcccgggttg
    41341 gggatttttg atggcatcat tgatgcaatt tggcgtgatt ttcacggcag gcatcacgct
    41401 gttccccttt gtcatgccgt caagtgtgag tccaatctcc agcctgacgt tgtgggacag
    41461 cacttccagt cagctgacgc tgagcattat gttggtaatc gtgctgatat ttttgcccat
    41521 tgtgttgctc tacactctct ggagctacta caaaatgtgg ggacgcatga caacagaaac
    41581 tctccgccgt aacgaaaacg agttgtacta aggagcagga acatgtggta tttactttgg
    41641 ttcgtcggca ttttgttgat gtgttcgctc tccacccttg tgttggtatg gctggacctg
    41701 cgtctgaaaa gttaacgaac gtaggcctga tgcggcgcat tagcatcgca tcaggcaatc
    41761 aataatgtta gatatgaaaa gcggaaacat atcgatgaaa gcgatcttaa tcccattttt
    41821 atctcttctg attccgttaa ccccgcaatc tgcattcgct cagagtgagc cggagctgaa
    41881 gctggaaagt atggtgattg tcagtcgtca tggtgtgcgt gctccaacca aggccacgca
    41941 actgatgcag gatgtcaccc cagacgcatg gccaacttgg ccggtaaaac tgggttggct
    42001 gacaccgcgc ggtggtgagc taatcgccta tctcggacat taccaacgcc agcgtctggt
    42061 agccgacgga ttgctggcga aaaagggctg cccgcagtct gctcaggtcg cgattattgc
    42121 tgatgtcgac gagcgtaccc gtaaaacagg cgaagccttc gccgccgggc tggcacctga
    42181 ctgtgcaata accgtacata cccaggcaga tacgtccagt cccgatccgt tatttaatcc
    42241 tctaaaaact ggcgtttgcc aactggataa cgcgaacgtg actgacgcga tcctttgcag
    42301 ggcaggaggg tcaattgctg actttaccgg gcatcggcaa acggcgtttc gcgaactgga
    42361 acgggtgctt aatttcccgc aatcaaactt gtgccttaac cgtgagaaac aggacgaaag
    42421 ctgttcatta acgcaggcat taccatcgga actcaaggtg agcgccgaca atgtctcatt
    42481 aaccggtgcg gtaagcctcg catcaatgct gacggagata tttctcctgc aacaggcaca
    42541 aggaatgccg gagccggggt ggggaaggat caccgattca caccagtgga acaccttgct
    42601 aagtttgcat aacgcgcaat tttatttact acaacgcacg ccggaggttg cccgcagtcg
    42661 cgccaccccg ttattggatt tgatcatggc agcgttgacg ccccatccac cgcaaaaaca
    42721 ggcgtatggt gtgacattac ccacttcagt gctgtttatt gccggacacg atactaatct
    42781 ggcaaatctc ggcggcgcac tggagctcaa ctggacgctt ccaggtcagc cggataacac
    42841 gccgccaggt ggtgaactgg tgtttgaacg ctggcgtcgg ctaagcgata acagccagtg
    42901 gattcaggtt tcgctggtct tccagacttt acagcagatg cgtgataaaa cgccgctgtc
    42961 attaaatacg ccgcccggag aggtgaaact gaccctggca ggatgtgaag agcgaaatgc
    43021 gcagggcatg tgttcgttgg caggttttac gcaaatcgtg aatgaagcac gcataccggc
    43081 gtgcagtttg taatgcataa aaaagagcat tcagttacct gaatgctctg aggctgatga
    43141 caaacgaaga actgtctaat gcgtaga
//
LOCUS       DAJTYF010000019        42601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTYF010000019 DAJTYF010000000
VERSION     DAJTYF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.019"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..636
                     /locus_tag="OO590_001177"
     CDS             <1..636
                     /locus_tag="OO590_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6636622.1"
                     /translation="SKLAALPNMPGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTS
                     CLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPA
                     KVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFIT
                     FVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            657..1315
                     /gene="csiR"
                     /locus_tag="OO590_001178"
                     /pseudo
     CDS             657..1315
                     /gene="csiR"
                     /locus_tag="OO590_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1316..1765)
                     /gene="kbp"
                     /locus_tag="OO590_001179"
     CDS             complement(1316..1765)
                     /gene="kbp"
                     /locus_tag="OO590_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6636623.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1849..2007)
                     /locus_tag="OO590_001180"
     CDS             complement(1849..2007)
                     /locus_tag="OO590_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6636624.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2190..2489
                     /locus_tag="OO590_001181"
     CDS             2190..2489
                     /locus_tag="OO590_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6636625.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2499..3023
                     /gene="ygaP"
                     /locus_tag="OO590_001182"
     CDS             2499..3023
                     /gene="ygaP"
                     /locus_tag="OO590_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6636626.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(3070..3474)
                     /gene="stpA"
                     /locus_tag="OO590_001183"
     CDS             complement(3070..3474)
                     /gene="stpA"
                     /locus_tag="OO590_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6636627.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4142..4579
                     /gene="alaE"
                     /locus_tag="OO590_001184"
     CDS             4142..4579
                     /gene="alaE"
                     /locus_tag="OO590_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6636628.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4616..4966)
                     /locus_tag="OO590_001185"
     CDS             complement(4616..4966)
                     /locus_tag="OO590_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6636629.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
     gene            5118..5447
                     /locus_tag="OO590_001186"
     CDS             5118..5447
                     /locus_tag="OO590_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6636630.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     QLSMRKS"
     gene            5695..5940
                     /gene="nrdH"
                     /locus_tag="OO590_001187"
     CDS             5695..5940
                     /gene="nrdH"
                     /locus_tag="OO590_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6636631.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            5937..6347
                     /gene="nrdI"
                     /locus_tag="OO590_001188"
     CDS             5937..6347
                     /gene="nrdI"
                     /locus_tag="OO590_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6636632.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            6320..8464
                     /gene="nrdE"
                     /locus_tag="OO590_001189"
     CDS             6320..8464
                     /gene="nrdE"
                     /locus_tag="OO590_001189"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6636633.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            8474..9433
                     /gene="nrdF"
                     /locus_tag="OO590_001190"
     CDS             8474..9433
                     /gene="nrdF"
                     /locus_tag="OO590_001190"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6636634.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            9788..10990
                     /gene="proV"
                     /locus_tag="OO590_001191"
     CDS             9788..10990
                     /gene="proV"
                     /locus_tag="OO590_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6636635.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            10983..12047
                     /gene="proW"
                     /locus_tag="OO590_001192"
     CDS             10983..12047
                     /gene="proW"
                     /locus_tag="OO590_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6636636.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            12105..13097
                     /gene="proX"
                     /locus_tag="OO590_001193"
     CDS             12105..13097
                     /gene="proX"
                     /locus_tag="OO590_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6636637.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            13289..14468
                     /locus_tag="OO590_001194"
                     /pseudo
     CDS             13289..14468
                     /locus_tag="OO590_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14592..15329
                     /gene="ygaZ"
                     /locus_tag="OO590_001195"
     CDS             14592..15329
                     /gene="ygaZ"
                     /locus_tag="OO590_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6636638.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            15319..15654
                     /gene="ygaH"
                     /locus_tag="OO590_001196"
     CDS             15319..15654
                     /gene="ygaH"
                     /locus_tag="OO590_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6636639.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            15745..16275
                     /gene="emrR"
                     /locus_tag="OO590_001197"
     CDS             15745..16275
                     /gene="emrR"
                     /locus_tag="OO590_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6636640.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            16402..17574
                     /gene="emrA"
                     /locus_tag="OO590_001198"
                     /pseudo
     CDS             16402..17574
                     /gene="emrA"
                     /locus_tag="OO590_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            17591..19129
                     /gene="emrB"
                     /locus_tag="OO590_001199"
     CDS             17591..19129
                     /gene="emrB"
                     /locus_tag="OO590_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6636641.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(19193..19708)
                     /gene="luxS"
                     /locus_tag="OO590_001200"
     CDS             complement(19193..19708)
                     /gene="luxS"
                     /locus_tag="OO590_001200"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6636642.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(19858..21414)
                     /gene="gshA"
                     /locus_tag="OO590_001201"
     CDS             complement(19858..21414)
                     /gene="gshA"
                     /locus_tag="OO590_001201"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6636643.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(21487..21915)
                     /locus_tag="OO590_001202"
                     /pseudo
     CDS             complement(21487..21915)
                     /locus_tag="OO590_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
     gene            complement(21912..22478)
                     /gene="yqaB"
                     /locus_tag="OO590_001203"
     CDS             complement(21912..22478)
                     /gene="yqaB"
                     /locus_tag="OO590_001203"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6636644.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(22626..22702)
                     /locus_tag="OO590_001204"
     tRNA            complement(22626..22702)
                     /locus_tag="OO590_001204"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22666..22668),aa:Arg,seq:acg)
     gene            complement(22767..22843)
                     /locus_tag="OO590_001205"
     tRNA            complement(22767..22843)
                     /locus_tag="OO590_001205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22807..22809),aa:Arg,seq:acg)
     gene            complement(22847..22939)
                     /locus_tag="OO590_001206"
     tRNA            complement(22847..22939)
                     /locus_tag="OO590_001206"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22903..22905),aa:Ser,seq:gct)
     gene            complement(23255..23440)
                     /gene="csrA"
                     /locus_tag="OO590_001207"
     CDS             complement(23255..23440)
                     /gene="csrA"
                     /locus_tag="OO590_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6636645.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(23675..26305)
                     /gene="alaS"
                     /locus_tag="OO590_001208"
     CDS             complement(23675..26305)
                     /gene="alaS"
                     /locus_tag="OO590_001208"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6636646.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(26433..26933)
                     /gene="recX"
                     /locus_tag="OO590_001209"
     CDS             complement(26433..26933)
                     /gene="recX"
                     /locus_tag="OO590_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6636647.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(27176..28237)
                     /gene="recA"
                     /locus_tag="OO590_001210"
     CDS             complement(27176..28237)
                     /gene="recA"
                     /locus_tag="OO590_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6636648.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(28317..28814)
                     /gene="pncC"
                     /locus_tag="OO590_001211"
     CDS             complement(28317..28814)
                     /gene="pncC"
                     /locus_tag="OO590_001211"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6636649.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(28959..30044)
                     /gene="mltB"
                     /locus_tag="OO590_001212"
     CDS             complement(28959..30044)
                     /gene="mltB"
                     /locus_tag="OO590_001212"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6636650.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            30300..30863
                     /gene="srlA"
                     /locus_tag="OO590_001213"
                     /gene_synonym="gutA"
     CDS             30300..30863
                     /gene="srlA"
                     /locus_tag="OO590_001213"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR6636651.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            30860..31819
                     /gene="srlE"
                     /locus_tag="OO590_001214"
                     /gene_synonym="gutE"
     CDS             30860..31819
                     /gene="srlE"
                     /locus_tag="OO590_001214"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6636652.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            31830..32201
                     /gene="srlB"
                     /locus_tag="OO590_001215"
                     /gene_synonym="gutB"
     CDS             31830..32201
                     /gene="srlB"
                     /locus_tag="OO590_001215"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6636653.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            32205..32984
                     /gene="srlD"
                     /locus_tag="OO590_001216"
     CDS             32205..32984
                     /gene="srlD"
                     /locus_tag="OO590_001216"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6636654.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            33089..33448
                     /gene="gutM"
                     /locus_tag="OO590_001217"
     CDS             33089..33448
                     /gene="gutM"
                     /locus_tag="OO590_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6636655.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            33515..34288
                     /gene="srlR"
                     /locus_tag="OO590_001218"
                     /gene_synonym="gutR"
     CDS             33515..34288
                     /gene="srlR"
                     /locus_tag="OO590_001218"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6636656.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            34281..35246
                     /gene="gutQ"
                     /locus_tag="OO590_001219"
     CDS             34281..35246
                     /gene="gutQ"
                     /locus_tag="OO590_001219"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6636657.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSSTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(35243..36757)
                     /gene="norR"
                     /locus_tag="OO590_001220"
     CDS             complement(35243..36757)
                     /gene="norR"
                     /locus_tag="OO590_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6636658.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGNDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            36944..38383
                     /gene="norV"
                     /locus_tag="OO590_001221"
     CDS             36944..38383
                     /gene="norV"
                     /locus_tag="OO590_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6636659.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            38380..39513
                     /gene="norW"
                     /locus_tag="OO590_001222"
     CDS             38380..39513
                     /gene="norW"
                     /locus_tag="OO590_001222"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6636660.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(39641..41893)
                     /gene="hypF"
                     /locus_tag="OO590_001223"
     CDS             complement(39641..41893)
                     /gene="hypF"
                     /locus_tag="OO590_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6636661.1"
                     /translation="MAKNTFCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(42046..42573)
                     /gene="hydN"
                     /locus_tag="OO590_001224"
     CDS             complement(42046..42573)
                     /gene="hydN"
                     /locus_tag="OO590_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6636662.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISCDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
BASE COUNT        10145 a        11323 c        10761 g        10372 t
ORIGIN      
        1 agtaagttgg cagcattacc gaatatgccg gggctgaaag ccgttggatc ttatcgctcg
       61 gttctggaat tgctcaatat tccccatgcg aaattaatca tggactgtgt gatattactt
      121 tccgtaacca gttgcctgaa ctcggccctg tataccgcct caaggatgct ctactcctta
      181 agccgtcgcg gtgatgctcc cgcggtaatg ggtaaaatca accgcagtaa aaccccgtac
      241 gtggcggtgt tactctccac cggcgcagca ttcttaacgg tggtggtgaa ctattacgcg
      301 cctgcgaagg tatttaaatt tctgatcgac agctccggcg ctatcgccct gctggtttat
      361 ttagtcatcg ccgtttcaca gttgcggatg cgcaaaattc tgcgagcaga aggaagcgaa
      421 attcgcttgc gaatgtggct ttatccctgg ctcacctggc tggtcatcgg ctttattacc
      481 tttgtgttgg tagtgatgct attccgtccg gcgcaacagt tagaagtgat ctccaccggc
      541 ttattagcga tagggattat ctgtaccgtg ccaattatgg ctcgctggaa aaagctggta
      601 ttgtggcaaa aaacacccgt tcataatacg cgctgataat gatcaggagt cacaccatga
      661 ccattacgtc tctggatggc tatcgctggc tgaagaacga tattattcgc ggtaattttc
      721 aaccggatga aaaattacgg atgagtttac tgacatcgcg ttatgcactt ggcgttgggc
      781 cgttgcggga agcactttct caactggtgg cggaacggct ggtcacggtg gtgaatcaaa
      841 aagggtatcg ggtggcgtct atgtcagagc aggagctgct ctgacataga cgccacccga
      901 tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga
      961 cgttctcgca aaagcgcatc tgctcaataa gctggaagcc tgtgacgcca gcgagaaaat
     1021 gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg
     1081 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt
     1141 tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac acgatcagca
     1201 ccagaccctg actgcggcgg tactggcgcg agatacagcg cgcgtcagtg agttaatgcg
     1261 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
     1321 cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt
     1381 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
     1441 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg
     1501 ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt
     1561 tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt
     1621 gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct
     1681 tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt tctccggcat
     1741 ctttcacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
     1801 tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt aatcgcgggt
     1861 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt
     1921 aataatgaac gcccaaccga accctttacc gagcagcacg ccgagcggcg gcagaatgat
     1981 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
     2041 aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag tttcagctta
     2101 cgcttttact ctcatttacg ctgctgaggc tggtatatgt tgcattttta gtttagagtt
     2161 tacttaattt agaaaatact taaataaata tgactgaact cgcgcaatta caggccagta
     2221 ccgaacaggc agcggcctta ttgaaagcaa tgagccaccc taaacggttg ctgattctgt
     2281 gcatgcttag cggttccccc ggcaccagcg cgggagagct gacgcgcatt accggactga
     2341 gcgcctctgc gacatcacag catctcgctc gtatgcggga cgaagggctt atcaacagcc
     2401 aacgggatgc ccaacgcatt ctatattcca ttaaaaatga ggcggtaaat gccattatcg
     2461 ccaccctgaa aaatgtctat tgtccgtaag gagtcactat ggctttgaca accatttcgc
     2521 cgcatgatgc acaagaatta atcgcacgcg gcgcaaagtt aatcgatatt cgtgacgctg
     2581 atgaatatct tcgtgaacat attcctgaag cagatctggc tccattatcc gtgctggaac
     2641 agtcaggtct tccacctaaa ttacgtcgcg agcaaattat attccactgc caggcaggta
     2701 aacgcaccag taataacgcc gataaattag cagcgattgc cgcccccgca gaaatatttt
     2761 tactcgaaga tgggattgat ggctggaaaa gagctggatt gccagtagcg gtaaataaat
     2821 ctcaaccctt gccgttaatg cgccaggtgc agatcgctgc gggtggttta atattaatcg
     2881 gcgttgtact gggttatacc gtaaatagcg gtttcttctt attaagtggc tttgttggtg
     2941 ccgggttact gtttgcagga atcagcggtt tttgtggaat ggcaaggttg ttagataaga
     3001 taccgtggaa ccaacgagct tgagaagcga cgccggacgc gccctagcag cgacatccgg
     3061 cctcagtaat tagatcagga aatcgtcgag agatttacct tctgccagcg cctgagcaat
     3121 tggcttcggt gtacggccct gaccggtcca ggttttagtt tcaccgttaa catcagtgaa
     3181 tttatatttc gccggacgcg gctggcgttt tttaccagca cgtggtgcag cagcagagct
     3241 attacccaat aactcttccg ggttaattcc gtcagctttc atcagctcca gccaggtgct
     3301 aattttttcc tgacgctcag ccagttcacg ctgctgctgt tcttcttctt cacgtctttc
     3361 tttagtgaca accctgaatt tttcgagcat ttcttcaaga acgtcaatgg agaattcgcg
     3421 agccatcgca cggagggtgc gaatgttatt taaactttgt aacattacgg acataataaa
     3481 gaaaaccttt taacgccaaa acaaaaaagt atttcgtcgc gtagtgtaag cgatcactta
     3541 ttatatttcc acaaaattta atctatttac agtcctgcgc gagattattt cagctgttat
     3601 tttctgaaac tctcagaata atttcaagat tatatattag ccattttgat gagtatttta
     3661 ggttattggt gaacagagtg gcgctctctg ctcgctaact ccgtaagcct gacaaaaccg
     3721 aagtgaataa aaatgcaata taacagatta aaagaaaaca cccctcactg gtgctttctg
     3781 ctgttaatgt acattttctg gcatcaaaaa tgctgtaaat gacccggtca cgacaaacac
     3841 cgaattctct acgcttatag cgttacctcc cccccaaact ttcattagtg aaattaaagg
     3901 caaggccata tccaattgat ttaattgaaa ataaatcata tcggcatgta aaaatccaca
     3961 atgtacaaaa aacgaaccgt tacggaatat tttatctaca aaagctgact aaataaaaat
     4021 ttttcactaa tttattagtc atagccagcg atatacgcta tgcgaaaata cagatggcaa
     4081 tgagatccac tgctttcatc tccattaaca tcccattacg cttttattaa ggagcattag
     4141 catgttctca ccgcagtcac gcttgcgtca tgcagttgca gatacgttcg cgatggttgt
     4201 ttactgttct gtcgtgaaca tgtgtattga agttttcctc tccggaatga gcttcgaaca
     4261 gtctttttat tccagattgg tagcgattcc ggtgaacatc ttaattgcat ggccatacgg
     4321 tatgtaccgc gatctgttta tgcgcgcggc acgcaaaggc tggataaaaa atctggctga
     4381 tatcctggct tatgtgacgt tccagtcacc ggtgtatgtg gcgatcttgt tagtggtggg
     4441 cgcagactgg catcagatta tggcggcggt cagttcaaac atcgttgttt cgatgttgat
     4501 gggggcggtt tatggctact tcctcgatta ttgccgccga ctgtttaaag tcagccgtta
     4561 ccagcaggta aaagcctgac tcttcttttc gcgactggca tcgccagtcg cgtctttaac
     4621 tggcttcgcc aaataaacca ttcaaataac gttcaagcgc aacacgcgaa ctaaagccat
     4681 gcgggatacc gtaatgctcg tgtcgctgtc gctcgcctgc taaatagagc ggaaattgct
     4741 ggtaatgatc gcaagtttta atatcggaag cgggttcagc cagtgttgaa ctgcgttctt
     4801 ttaatgttgg atgaattacc agagcggtac gtcccattcg cgcttcgcga ttaacataga
     4861 cataattttc cccacggcgg taaccatacg ctttctgggt taccacatcg acagtaaaac
     4921 cgactttttc aagtacgcgc gccacctcgt ctggtcgtaa atacatattt gatcctcgtt
     4981 attttcaacc gcgggcctac cttacctgat tgcgcattca caacgctttc agaaaagtcc
     5041 ataaagccgc gatgaacctc agttaagaaa attatggtct acactgaaaa ttacatcgaa
     5101 ttctaatggg ggatcatatg tttaaccgac cgaaccgcaa cgacgttgat gatggcgtgc
     5161 aggatatcca gaatgatgtc aatcaattag ctgacagcct cgaatctgta ttgaagtcct
     5221 ggggcagcga cgccaaaggg gaagctgaag ccgcacgcag taaagctcag gcgttgctga
     5281 aagaaaccag ggcgcgaatg catggtcgta ctcgcgtcca gcaagccgcg cgcgatgccg
     5341 ttggctgcgc ggattctttt gttcgtgaaa gaccctggtg tagcgtgggt acagcagctg
     5401 cggtaggtat ttttatcggc gcacagttaa gcatgcgcaa atcgtagtgc aaaaatgata
     5461 ataaatacgc gtctttgacc ccgaagcctg tcttcggggt ttctttttgc ctggtgaatc
     5521 acaaaaatcc ccctcccccg tcacgctcat atccagggta atttcgatca ctatttgcta
     5581 tatattgtgt ggttgaatct tttttcaact acatctagta tctctgtatc aacagagaga
     5641 caacccgacg cgtatcatcg cgccgtatct tcattttaaa cagaaatacg aatcatgcgc
     5701 ataactattt atactcgtaa cgattgcgtt cagtgccacg ccaccaaacg ggcgatggaa
     5761 aaccggggct ttgattttga aatgattaat gtcgatcgcg ttcctgaagc agcggaagcg
     5821 ttgcgtgctc agggctttcg tcagttgccg gtagtgattg ctggcgatct tagctggtct
     5881 ggtttccgtc cggacatgat taaccgtctg catccagcgc cacacgcggc cagtgcatga
     5941 gccagctcgt ctacttctcc agcagctccg aaaacacgca gcgttttatc gaacgtttag
     6001 gtctgcccgc ggtgcgcatc ccgctcaatg agcgggaacg gattcaggta gacgagcctt
     6061 acatcctgat cgtgccctct tacggcggcg gcggtacggc tggcgcggtg ccacgacagg
     6121 taattcgctt tttaaacgac gagcacaacc gggcgttgct tcgcggcgtt attgcttctg
     6181 gtaatcgcaa ctttggtgag gcgtatggcc gcgccggaga tgtgattgcc cggaaatgcg
     6241 gcgtgccgtg gctgtaccgt tttgaactca tgggtacgca aagcgatatc gaaaacgttc
     6301 gtaaaggagt aaccgaattt tggcaacgac aaccgcagaa tgcctgacgc aggaaacgat
     6361 ggattaccac gcgctgaatg cgatgcttaa tctttacgat agcgcaggtc gcattcagtt
     6421 cgataaagac cgccaggccg ttgacgcctt tattgcgacg catgtgcgtc cgaacagtgt
     6481 gaccttcagt agccagcagc agcgcctgaa ctggctggtc aacgaaggtt actatgatga
     6541 aagcgttctt aatcgctact ctcgcgactt tgtcattacg ctgtttgccc acgcacacac
     6601 cagcggtttt cgtttccaga cattcctcgg ggcatggaag ttttacacca gctatacgtt
     6661 gaagacattc gacggtaaac gttatctgga agattttgcc gatcgagtca cgatggtggc
     6721 gctgacgctg gcacaaggcg atgagacgct ggcgttgcaa ctgaccgatg aaatgctgtc
     6781 aggacgcttt cagccagcca cgccaacatt cctcaactgc ggtaagcagc agcgcggcga
     6841 actggtttcc tgttttttgc tgcgtattga agacaatatg gagtcgattg gtcgggcggt
     6901 aaattccgca ctgcagctgt cgaaacgcgg cggcggcgta gcatttttgc tgtcgaatct
     6961 gcgggaagcg ggcgcgccaa ttaaacgtat tgaaaatcaa tcttctggcg taattccggt
     7021 gatgaaaatg ttggaagacg cattttccta tgccaaccaa ctcggcgcgc gtcagggggc
     7081 aggtgcagtc tatttacatg ctcatcatcc cgatattctg cgttttctcg acacgaaacg
     7141 ggaaaatgcc gacgaaaaaa tccgcattaa aacactgtcg ctgggcgtgg tgatcccgga
     7201 tatcactttc catctggcaa aagagaatgc gcagatggcg ctgttttcgc cctatgacgt
     7261 tgagcgagtt tatggcaagc cgtttgccga tatcgccatt agcgaacact atgacgaact
     7321 ggttgccgat gaacgcatcc gcaaaaaata cctcaacgcc cgtgatttct tccagcgact
     7381 ggcagaaatc cagtttgagt ccggctatcc ctacatcatg tatgaagaca cagtaaaccg
     7441 tgctaaccct atcgccgggc gcataaatat gagtaatctc tgctcagaaa ttttgcaggt
     7501 taacagcgcc tcagagtatg acgagaatct cgactatgcc cgcacaggcc atgatatttc
     7561 ctgcaattta ggttcgttga atattgcgca caccatggat tcccccgatt tttcccgcac
     7621 ggtagagact gccgtgcgcg gtttaacggc ggtgtcagat atgagtcata tccgcagcgt
     7681 gccgtccatc gaagccggaa atgccgcctc gcacgccatc ggactggggc agatgaattt
     7741 acatggttat ctggcgaggg aaggcatcgc ttatggttcg ccggaagccc tggatttcac
     7801 caatctctat ttctatacca tcacctggca cgcactgcgt acctcgatgt tgctggcgcg
     7861 cgaacgcggt gaaaccttcg ccgggttcaa acagtcacgc tatgccagtg gtgaatattt
     7921 cagccaatat ctgcaaggta actggcagcc gaaaacggcg aaagttggcg aactgtttgc
     7981 ccgcagcggt attacgttac ctacccgtga gatgtgggcg caactgcgcg acgacgtgat
     8041 gcgctacggc atatacaacc agaatctcca ggcggtgccg ccaaccgggt ctatctctta
     8101 tatcaaccat gctacgtcga gtattcatcc gattgtggcg aaagtagaga tacgcaaaga
     8161 gggcaaaaca ggacgcgttt actaccctgc cccgtttatg actaacgaaa atctggcgct
     8221 gtatcaggac gcttacgaaa ttggcgcaga aaagatcatc gacacctacg cggaagcgac
     8281 tcgccatgtc gatcaggggc tgtcgctgac gctgtttttc cccgataccg ccaccactcg
     8341 cgatatcaac aaagcgcaga tttacgcctg gcgcaaaggt atcaaaacgc tctattacat
     8401 ccgcctgcgt cagatggcgc tggaaggcac tgaaattgaa ggctgcgtct cctgtgcact
     8461 ttaaggaata tctatgaaac tctcacgtat cagcgccatc aactggaaca agatatctga
     8521 cgataaagat ctggaggtgt ggaatcgcct gaccagcaat ttctggctac cagaaaaggt
     8581 gccgctgtcg aacgatattc ccgcctggca gacattaact gtcgtagaac aacaactgac
     8641 gatgcgcgtt tttactggcc tgacgctgct cgacacgctg caaaatgtta tcggcgcgcc
     8701 ttcgctgatg cccgatgcgc tcacgcctca tgaagaggcg gtattatcga atatcagctt
     8761 tatggaagcg gttcatgccc gctcttacag ttcgattttc tcgacgctgt gccagaccaa
     8821 agatgtcgat gccgcctacg cctggagtga agaaaacgca ccgttgcagc gaaaagctca
     8881 gattattcag caacattatc gcggtgatga tccgctgaaa aagaaaatcg ccagtgtgtt
     8941 tcttgaatct tttttgttct attccggttt ctggctgccg atgtattttt ccagccgcgg
     9001 aaagctgacc aataccgcgg acctgatccg tctgattatc cgcgatgaag cagtccacgg
     9061 ttactacata ggctataaat atcagaaaaa catggaaaag atatctctgg gacaacgtga
     9121 agagttgaag agtttcgcct tcgatttgtt gctggaactc tacgacaacg agttgcaata
     9181 caccgatgag ctgtacgccg aaaccccgtg ggctgacgat gtgaaagcgt ttctctgtta
     9241 caacaccaat aaggctttga tgaatctggg ctacgaaccg ttatttcccg cagaaatggc
     9301 ggaagtgaat ccggcaatcc tcgccgcgct ttcgccgaat gccgatgaaa atcacgattt
     9361 cttttccggt tcaggctcct cttatgtgat ggggaaagcg gttgaaacag aagatgaaga
     9421 ctggaatttc tgagggtgtt attttcaaaa atatcactac ccgcagcagg gaaataattc
     9481 ccgccaaata gctttttatc acgcaaatat tctgtagtga tctacactga tactctgttg
     9541 cattattcgc ctgaaactac aatattcagg cgttttttcg ctatctatga caaaaaatat
     9601 caacttttct cgatttgctc tcagccctta tatcacggaa aattccggcg atttgctcgc
     9661 atcaatattc atgccacatt tgccctcagg ggttgcctca gattctcagt atgttagggt
     9721 agaaaaaagt gactattccc attgggtaat atatcgacat aaacaaataa aggaatcatt
     9781 ctattgcatg gccattaaat tagaaattaa aaatctttat aaaatatttg gtgagcatcc
     9841 acagcgagcg ttcaaatata tcgaacaagg actttcaaaa gaacaaattc tggaaaaaac
     9901 tgggctatcg cttggcgtaa aagacgccag tctggccatt gaagaaggcg agatatttgt
     9961 catcatggga ttatccggct cgggtaaatc cacaatggta cgccttctca atcgcctgat
    10021 tgaacccacc cgcgggcaag tgctgattga tggtgtggat attgccaaaa tatccgacgc
    10081 cgaactccgt gaggtgcgca gaaaaaagat tgcgatggtc ttccagtcct ttgccttaat
    10141 gccgcatatg accgtgctgg acaatactgc gttcggtatg gaattggccg gaattaatgc
    10201 cgaagaacgc cgggaaaaag cccttgatgc actgcgtcag gtcgggctgg aaaattatgc
    10261 ccacagctac ccggatgaac tctctggcgg gatgcgtcaa cgtgtgggat tagcccgcgc
    10321 gttagcgatt aatccggata tattattaat ggacgaagcc ttctcggcgc tcgatccatt
    10381 aattcgcacc gagatgcagg atgagctggt aaaattacag gcgaaacatc agcgcaccat
    10441 tgtctttatt tcccacgatc ttgatgaagc catgcgtatt ggcgaccgaa ttgccattat
    10501 gcaaaatggt gaagtggtac aggtcggcac accggatgaa attctcaata atccggcgaa
    10561 tgattatgtc cgtaccttct tccgtggcgt tgatattagt caggtgttca gtgcgaaaga
    10621 tattgcccgc cggacaccga atggcttaat tcgtaaaact cctggcttcg gcccacgttc
    10681 ggcactgaaa ttattgcagg atgaagatcg cgaatatggc tacgttatcg aacgcggtaa
    10741 taagtttgtc ggcgcagtct ccatcgattc gcttaaaacc gcgttaacgc agcagcaagg
    10801 tcttgatgcg gcgctgattg atgcgccgtt agcagtcgat gcacaaacgc ctcttagcga
    10861 gttgctctct catgtcggac aggcaccctg tgcggtgccc gtggtcgacg aggaccaaca
    10921 gtatgtcggc atcatttcga aaggaatgct gctgcgcgct ttagatcgtg agggggtaaa
    10981 taatggctga tcaaaataat ccgtgggata ccacgccagc ggcggacagt gccgcgcaat
    11041 ccgcagacgc ctgggggaca ccgacgactg caccggctga cggcggtggt gctgactggc
    11101 tgaccagtac gcctgcgcca aacgtcgagc attttaatat tctcgatccg ttccataaaa
    11161 cgctgatccc gctcgacagt tgggtcactg aagggatcga ctgggttgtt acccatttcc
    11221 gtcccgtctt ccagggcgtg cgcgttccgg ttgattatat cctcaacggt ttccagcaat
    11281 tgctgctggg tatgccagcg ccagtggcga ttatcgtttt cgctctcatc gcctggcaga
    11341 tttccggggt cggaatgggc gtggcgacgc tggtctcgct gattgccatc ggcgcaatcg
    11401 gtgcctgttc gcaggcaatg gtgactctgg cgctggtgtt aactgccctg ctgttctgta
    11461 tcgtcatcgg tttgccgttg gggatatggc tggcgagaag tccgcgagcg gcgaaaatta
    11521 ttcgtccact gcttgatgcc atgcagacca cgccagcgtt tgtttatctg gtgccaatcg
    11581 tcatgctatt tggtatcggt aacgtgccgg gcgtggtggt gacgatcatc tttgcgctgc
    11641 cgccgattat ccgtctgacc attctgggga ttaaccaggt tccggcggat ctgattgaag
    11701 cctcgcgctc attcggtgcc agcccgcgcc agatgctgtt caaagttcag ttaccgctgg
    11761 cgatgccgac cattatggcg ggcgttaacc agacgctgat gctggccctt tctatggtgg
    11821 tcattgcctc gatgattgcc gtcggcgggc tgggtcagat ggtacttcgc ggtatcggtc
    11881 gtctggatat ggggcttgcc accgttggcg gcgtcgggat tgtgatcctc gccattatcc
    11941 tcgatcgcct gacgcaggcc gttgggcgcg actcacgcag tcgcggcaac cgtcgctggt
    12001 acaccactgg ccctgttggt ctgctgaccc gcccattcat taagtaactc tgcacttgcc
    12061 cggtgacgcc gggcattatc accctgccaa aaaaaggaat aacaatgcga catagcgtac
    12121 tttttgcgac agcgtttgcc acgcttatct ctacacaaac ttttgctgcc gatctgccgg
    12181 gcaaaggcat tactgttaat ccagttcaga gcaccatcac tgaagaaacc ttccagacgc
    12241 tgctggtcag tcgtgcgctg gagaaattag gttataccgt caacaaaccc agcgaagtgg
    12301 attacaacgt tggctacacc tcgcttgctt ccggcgatgc aaccttcacc gccgtgaact
    12361 ggacgccact ccatgacaac atgtacgaag ctgccggtgg cgataagaaa ttttatcgtg
    12421 aaggggtatt tgttaacggc gcagcacagg gttacctgat cgataagaaa accgccgacc
    12481 aatacaaaat caccaacatc gcacaactga aagatccgaa gatcgccaaa ctgttcgata
    12541 ccaacggcga cggtaaagcg gatttaaccg gttgtaaccc tggctggggc tgcgaaggtg
    12601 cgatcaacca ccagcttgcc gcgtatgaac tgaccaacac cgtgacgcat aatcagggga
    12661 actacgcagc gatgatggcc gacaccatca gtcgctacaa agagggcaaa ccggtgtttt
    12721 attacacctg gacgccgtac tgggtgagta atgagctgaa gccagggaaa gatgtggtct
    12781 ggttgcaggt gccgttctcc gcactgccgg gcgataaaaa tgccgatacc aaactgccga
    12841 atggcgcgaa ttacggcttc ccggtcagca ccatgcatat cgttgccaac aaagcctgga
    12901 ccgagaaaaa tccggcggca gccaaactgt ttgccattat gcagttgcca gtggccgata
    12961 ttaacgccca gaacgccatt atgcatgacg gcaaagcctc agaaggcgat attcagggcc
    13021 acgttgatgg ctggatcaaa gcccaccagc agcagttcga tggctgggtg aatgaggcgc
    13081 tggcagcgca gaagtaattt ttattcgggc ggataaggcg ttttcgcctc atccgccgct
    13141 ctgtacacaa tgcctgatgc gacgctgacg cgtcctatca tgcctacaaa ccacatcgca
    13201 catttcaaca agctatcttt catcccatat tcatcaacat ccgctattat tgatttccag
    13261 cttaatcatc acctgatgaa caaaaataat gactaaacct aatcatgagc ttagcccggc
    13321 gctgatcgtg ctgatgtcta tcgccaccgg tctggcggta gccagtaact attacgccca
    13381 gccattgctc gacaccatcg cgcgtaactt ttccctttcc gccagttcgg caggctttat
    13441 tgttaccgcc gcgcagttgg gctatgccgc aggtctactg tttcttgttc ccctcggtga
    13501 tatgtttgaa cgccgccgcc tgattgtctc gatgacctta ctggcagcgg gcggtatgtt
    13561 aatcactgcc agcagtcagt cactggcgat gatgatcctc ggtacggcat taaccggttt
    13621 attctcagtc gtggcacaaa ttctggttcc gctggcagcg acgctggctt caccggataa
    13681 acgcggcaaa gtggttggca ccattatgag cggtctgctg ttggggatat tgctggcacg
    13741 aaccgttgcc gggttactgg caaacctcgg cggctggcgc accgtctttt gggttgcttc
    13801 ggtgttaatg gcactgatgg cgctggcatt atggcgtggt ctgccacaaa tgaaatcaga
    13861 aacccacctc aactacccac agttgttggg ttccgttttc agcatgttca tcagcgataa
    13921 gatcctgcgc acccgcgcgt tgctgggctg catgaccttt gccaacttca gcattctctg
    13981 gacctcaatg gcctttttgc ttgccgctcc accttttaac tacagcgatg gtgacattgg
    14041 tctgtttggg cttgcgggag ctgccggagc gttgggcgct cgtccggcgg gcggttttgc
    14101 cgacaagggc aaatcgcacc acaactttcg gtctgcttct gctgttactt tcatggctgg
    14161 caatctggtt tggtcacact tccgtactgg cgttgattat cggcattctg gtgctggacc
    14221 tcaccgtaca gggcgtgcat atcactaacc agacggtaat ttatcgaata catcctgatg
    14281 cgcgtaatcg cctgaccgca ggttacatga ccagctactt tattggcggt gccgccggtt
    14341 cgctaatttc agcctcagcc tggcaacatg gcggttgggc tggcgtttgt ctggctggcg
    14401 cgacgattgc cctggttaac ttactggtct ggtggcgagg ttttcatcgt caggaagccg
    14461 caaattaagc aatcagcaac cgttttcgtg gctttacact tataagggtg ttaagaagcc
    14521 catcagtctg ataaggttaa gatattcatt cagtctattt ataatattaa caatcgttaa
    14581 gcgtacactc tatggaaagc cctactccac agcctgctcc tggttcggcg accttcatgg
    14641 aaggatgcaa agacagttta ccgattgtta ttagttatat tccggtggcc tttgcgttcg
    14701 gtctgaatgc gacccgtctg ggattctctc ctctcgaaag cgtttttttc tcctgcatca
    14761 tttatgcagg cgcgagccag ttcgtcatta ccgcgatgct ggcagccggg agtagtttgt
    14821 gggttgctgc actgaccgtc atggcaatgg atgttcgcca tgttttgtat ggcccgtcac
    14881 tgcgtagccg tattattcag cgtctgcaaa aatcgaaaac tgccctatgg gcgtttggcc
    14941 tgacggatga ggtttttgcc gccgccaccg caaaactggt acgcaataat cgccgctgga
    15001 gcgagaactg gatgatcggc attgccttca gttcatggtc atcgtgggta tttggtacgg
    15061 taataggggc attctccggc agcggcttgc tgcaaggtta tcccgccgtt gaagctgcat
    15121 taggttttat gcttccggca ctctttatga gtttcctgct cgcctctttc cagcgcaaac
    15181 aatctctttg cgttaccgca gctttagttg gtgcccttgc aggcgtaacg ctattttcta
    15241 ttcccgtcgc cattctggca ggcattgtct gtggctgcct cactgcgtta atccaggcat
    15301 tctggcaagg agcgcccgat gagctatgag gttctgctgc ttgggttact ggttggcgcg
    15361 gcgaattatt gcttccgcta tttgccgctg cgcctgcgtg tgggtaatgc ccgcccaacc
    15421 aaacgtggcg cggtaggtat tttgctcgac accattggca tcgcctcgat atgcgctctg
    15481 ctggttgtct ctaccgcacc agaagtgatg cacgatacac gccgtttcgt gcccacgctg
    15541 gtcggcttcg cggtactggg tgccagtttc tataaaacac gcagcattat catcccaaca
    15601 ctgcttagtg cgctggccta tgggctcgcc tggaaagtga tggcgattat ataacccaca
    15661 agaatcattt ttctaaaaca atacatttac tttatttgtc actgtcgtta ctatatcggc
    15721 tgaaattaat gaggtcatac ccaaatggat agttcgttta cgcccattga acaaatgcta
    15781 aaatttcgcg ccagccgcca cgaagatttt ccttatcagg agatccttct gactcgtctt
    15841 tgcatgcaca tgcaaagcaa gctgctggag aaccgcaata aaatgctgaa ggctcagggg
    15901 attaacgaga cgttgtttat ggcgttgatt acgctggagt ctcaggaaaa ccacagtatt
    15961 cagccttctg aattaagttg tgctcttgga tcatcccgta ccaacgcgac gcgtattgcc
    16021 gatgaactgg aaaaacgcgg ttggatcgaa cgtcgtgaaa gcgataacga tcgccgctgc
    16081 ctgcatctgc aattaacgga aaaaggtcac gagtttttgc gcgaggtttt accaccgcag
    16141 cataactgcc tgcatcaact ctggtccgcg ctcagcacaa cagaaaaaga tcagctcgag
    16201 caaatcaccc gcaaattgct ctcccgtctc gaccagatgg aacaagacgg tgtggttctc
    16261 gaagcgatga gctaacgcgt catctcgctc aaaaatccag atttataaaa gaaaaaatga
    16321 ccggccagca tcgcaacatg ctggcctttt tggcaagcag gtcggctcag ccgatgagtt
    16381 aagaagatcg tggagaacaa tatgagcgca aatgcggaga ctcaaacccc gcagcaaccg
    16441 gtaaagaaga gcggcaaacg taagcgtctg ctcctccttc tcaccttgct ctttataatt
    16501 attgccgtag cgatagggat ttattggttt ttggtactgc gtcatttcga agaaaccgat
    16561 gacgcatacg tggcagggaa tcagattcaa attatgtctc aggtgtctgg cagcgtgacg
    16621 aaagtctggg ccgataacac cgattttgta aaagaaggcg atgttctggt cactctcgac
    16681 ccgacagatg ctcgccaggc gtttgaaaaa gccaaaactg cactggcttc cagcgttcgc
    16741 caaacccacc agctgatgat taacagcaaa cagttgcagg cgaatattga ggtgcaaaaa
    16801 atcgccctcg cgaaagcaca aagcgactac aaccgccgtg tgccgctggg caatgccaac
    16861 ctgattggtc gcgaagagct gcaacacgcc cgcgacgccg tcaccagtgc ccaggcgcaa
    16921 ctggacgtcg ctattcaaca atacaatgcc aatcaggcga tgattctggg gactaaactg
    16981 gaagatcagc ctgccgtgca acaggctgcc accgaagtac gtaacgccta gctggcgctg
    17041 gagcgtaccc gtattgtcag tccgatgacc ggttatgttt cccgtcgtgc cgtgcaaccg
    17101 ggtgcgcaaa ttagcccaac gacgccgctg atggcggtcg ttccagccac caatatgtgg
    17161 gtggatgcca actttaaaga gacgcagatt gccaatatgc gcatcggtca gccggtcacc
    17221 atcaccaccg atatttacgg cgatgatgtg aaatacaccg gtaaagtggt tggtctggat
    17281 atgggcacag gtagcgcgtt ctcactgctt ccggcgcaaa atgcgaccgg taactggatc
    17341 aaagtcgttc agcgtctacc tgtgcgtatc gaactggacc agaaacagct ggagcaatac
    17401 ccgctgcgca ttggtttgtc cacgctggtg agcgtcaata ccactaaccg tgacggtcag
    17461 gtactggcaa ataaagtacg ttcaacaccg gtcgcggtaa gtaccgcgcg tgaaatcagc
    17521 ctagcacctg tcaataaact gatcgacgat atcgtaaaag ctaacgctgg ctaatccaga
    17581 ggtgcgtgtg atgcaacagc aaaaaccgct ggaaggcgcg caactggtca ttatgacgat
    17641 tgcgctgtca ctggcgacat tcatgcaggt gctggactcc accattgcta acgtggcgat
    17701 ccccactatc gccgggaatc tgggctcatc gctcagccaa ggaacgtggg taatcacttc
    17761 tttcggggtg gcgaatgcca tctcgatccc gcttaccggc tggctggcaa agcgcgtcgg
    17821 ggaagtgaaa ctgttccttt ggtccaccat cgcctttgct attgcgtcgt gggcgtgtgg
    17881 tgtctccagc agcctgaata tgctgatctt cttccgcgtg attcagggga ttgtcgccgg
    17941 gccgttgatc ccgctttcgc aaagtctatt gctgaataac tacccgccag ccaaacgctc
    18001 gatcgcgctg gcgttgtggt cgatgacggt gattgtcgcg ccaatttgcg gcccgatcct
    18061 cggcggttat atcagcgata attaccactg gggctggata ttcttcatca acgtgccgat
    18121 tggcgtggcg gtggtgttga tgacactgca aactctgcgc ggacgtgaaa cccgcaccga
    18181 acggcggcgg attgatgccg tggggctggc actgctggtt attggtatcg gcagcctgca
    18241 gattatgctc gaccgcggta aagagctgga ctgtttttca tcacaggaaa ttatcattct
    18301 taccgtggtg gcggtggtgg ctatctgctt cctgattgtc tgggagctga ccgacgataa
    18361 cccgatagtc gatctgtcgt tgtttaagtc gcgcaacttc accatcggct gcttgtgtat
    18421 cagcctcgcg tatatgctct acttcggcgc tattgttctg ctgccgcagt tgttgcagga
    18481 ggtctacggt tacacggcga cctgggcagg tttggcctct gcgccggtag ggattattcc
    18541 ggtgattctg tcgccgatta tcggccgctt cgcgcataaa ctggatatgc ggcggctggt
    18601 aaccttcagc tttattatgt atgccgtctg cttctactgg cgtgcctata cctttgaacc
    18661 aggtatggat tttggcgcgt cggcctggcc gcagtttatc caggggtttg cggtggcctg
    18721 cttctttatg ccgctgacca ccattacgct gtctggtttg ccaccggaac gactggcggc
    18781 ggcatcgagc ctctctaact ttacgcgaac gctggcgggg tctatcggca cgtcgataac
    18841 cacgaccatg tggaccaacc gcgagtcgat gcaccatgcg cagttgactg agtcggtaaa
    18901 cccgttcaac ccgaatgccc aggcgatgta cagtcaactg gaagggcttg ggatgacgca
    18961 acagcaggca tcaggctgga ttgcccagca gatcaccaat caggggctga ttatttccgc
    19021 caatgagatc ttctggatgt cagccgggat attcctcgta ctgctggggc tggtgtggtt
    19081 tgctaaaccg ccatttggcg caggtggcgg cggaggcggt gcgcactaag tacaactaag
    19141 ccagttcatt tgaactggct tttttcaatt aattgtgaag atagtttact gactagatgt
    19201 gcagttcctg caacttctct ttcggcagtg ccagttcttc gttgctgttg atgcgtacgt
    19261 cacgttccag aatgctacgc gcaatatcct gcgcttcctg caacgagtgc atctggtaag
    19321 tgccacactg gtagacgttc agttccggga tctgattctg atcctgcact ttcagcacgt
    19381 cttccattgc cgctttccag gcatcagcaa cacgctgctc atctggcgta ccaatcagac
    19441 tcatataaaa accggtgcgg cagcccattg gcgagatatc gataatctct acaccattac
    19501 cgttaagatg gttacgcata aaaccagcaa acaggtgctc cagggtatgg atccctcttt
    19561 ctggcatcac ttctttgttc ggcacgcaga agcgcagatc gaacacggtg attgcgtcgc
    19621 catgcggggt gttcattgtt ttcgccaccc gaactgcagg cgcttccatc cgggtatgat
    19681 cgactgtgaa gctatctaac aacggcattt agccacctcc ggtaattttt ttaaaaattt
    19741 tctgaactct ttcttcccag gcgagtctga gtatatgaaa gacgcgcatt tgttatcatc
    19801 atccctgaat tcagagatga aattttggcc actcacgagt ggcctttttc ttttctgtca
    19861 ggcgtgtttt tccagccaca ccgcaaacgg ttcggtatca gcggtttcca tttcctgctg
    19921 acggcgttca gatgcctcgc gctcggctac aaaatcctct tcgcgcagaa tttccagcgg
    19981 ctcttcacgc agcagattac ggtaggcttc tgcaaacgct ttgcctgttc cgccaatacc
    20041 agtatcaatc atagacctta agatacgggc agagaaagtc agatcgggat tatcgaagca
    20101 ggcaaccagt tcatcacaca ctttctgata cgcttcgccg ccgttaatgc tatccagcgt
    20161 ttgcgcgacg cgtttcagat cgtggaacag atctttaccc acctgcagta acgggaactg
    20221 cgcggtttcg cagccgatac ccagcgtcag gcccggtttg cgaccttcaa ggatcacccg
    20281 gttccagtta acgcgcgtac aggcaagttc gctactgctc atttccggtg catcagccag
    20341 cgcacaccag accataaaca ggtcgaggaa tcgcacctgc tgttcatcta caccaatcgg
    20401 cgagaacggg ttgatgtcca gcgaacgcac ttcaatatat tcaatgccgc cacgtaacag
    20461 cgcatcagaa ggtgactcgc cgctgcgggt aacgcgtttt ggacgaatcg gcgcatacag
    20521 ttcgttttca atctgcagca cgttgctatt gatttgcagc ctcttaccgt ctttctcaat
    20581 accaatcttc gcgtactctt ccgatggcgt tttgattgcc tgtttaaggc ccgctacgta
    20641 ctcgtaaaga tcgttgaagg taataccaag attgctttgc gatttattgg tatagccgag
    20701 atcgctcaaa cgaagagagg tcgcatacgg caggtaatac ataccgcact cggttttctc
    20761 aaacggcagc gacgttggtt ttccttgcag gaaagaagaa caaatcgccg gagatgcacc
    20821 aaacagataa ggaatgaccc aaccgaaacg atagtaattg cggataacgc ggaaatagcc
    20881 cgcagaaatt ttctctttgg catcagcgcc cgagatatca ccgcacttcg cttgccagaa
    20941 tgccattggc aaagagaaat tgtagtgcac gccggaaatg gtttgcatca gcgcgccgta
    21001 gcgatttttc agcccttcac gatacagcgt tttaaagcgt ccggtgttag aagtgccgta
    21061 ctgtgccagt tcgatgtcct gaccttctgc gatgtagcat ggcatactta acggccacat
    21121 ccgctcatcg cccatattgc gcgccgtata acgatgcaga tcgcgcataa aggtcagcat
    21181 atgttcaata tcaccatcca ctggtgtaat gaattccagc aatgcttccg caaaatcggt
    21241 agtaatccat ttgtgcgtca gtgcggaacc taatgcttca ggatgacctg ttgttgccag
    21301 tgtgccatca gcattaacac gcaaagtttc gcgctccagc ccacgctgta tcccctttaa
    21361 cgcctgagga tgtttttcca gccaggccag cgcctgtgat acgtccggga tcaaattgac
    21421 ctcccgcctg tcaaaatcgt tttaattagc ataactgtaa tggtgaccat atgtgcaggc
    21481 ctacaattag tgccaccaca tcatgccctg aacggtcgct gctgcaactg caacatagcg
    21541 taacgcttta ccaaggcaca aaaaaaagat tactggcccc caggagatgc gcatccatcc
    21601 cgctaacaga cacagtaaat cgccaaccac gggcatccag cttaataata gcgtgactgc
    21661 accatagcgt ttcagccagc cggtagcttt ctcttgccag cgcgatgttt tacgcaatgg
    21721 aaagaaacgc ccaaggataa cgttagttaa ccctccaagg ctattaccca ttgttgctgt
    21781 taaaactaaa acctagggat gactgatccc ggaaagcaac attgccacca gcacgacttc
    21841 ggagttgccg ggtaatagtg tagcgctgag aaaactactg gcaaacaacg agaaaagcga
    21901 taacgcttca ctcacagcaa gcgaacatcc acagcgtcca tgcctgctgc acgggccgcc
    21961 tgaataccga aatcggcatc ttcaaagacc acacactgcg tcggttgcac gcccatacgc
    22021 tgcgcgcaca acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg atcagcagcg
    22081 acgacggcgt caaaataacg gcgtaatccc aggtgcgcca gcaatgcctc tgcgatggcg
    22141 ctttcactcc ccgttcctac agccattggg cgacgaccat gccaactttt caccacttca
    22201 acaagaggaa gcggctcgac actatccagc agcatacttc ttactgcttc tgttttttca
    22261 cgcgctaacg catgcgggtc gagatcggcc tgattcagct caataattgc ctgagcaata
    22321 cgccaggtgg gcgatccatt aagcgcaatc atcgcctgaa tatcgtactg aagaccgtag
    22381 tgccctaata cttcgcgcca cgctttacgg tgcgtaggct ccgtatccag gattgtgcca
    22441 tccatatcaa aaattaaacc tgcataacgc tcgtacatgg tcttctcgca agtcgaaaaa
    22501 tatgacgcta ctttagcgta attgcttgat tttgtcgctg atggcgaagg agaagaaaat
    22561 tcaggaaatg gataaagtag ttatttagaa atgagatatt tttgaaggaa attttttgga
    22621 gaatatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc
    22681 tatccagctg agctacggat gcatcgggaa acttacttta ctgcagattt tttgataccg
    22741 ctactaaagc cgtatcaagt aagagatggt gcatccggga ggattcgaac ctccgaccgt
    22801 tcggttcgta gccgagtact ctatccagct gagctacgga tgcaaatggc ggtgaggcgg
    22861 ggattcgaac cccggatgca gcttttgacc gcatactccc ttagcagggg agcgccttca
    22921 gcctctcggc cacctcacca cacgcctctt acgagtgctt cgaagaactt gtttatcgct
    22981 catcgtcgct gcgtggcgca catattactt tctgagactt ataagtcaaa caatttttcc
    23041 cacactttta tcgtttgcac acttcacgct caattagtct gtaaaaacgt caaaaagagt
    23101 gttttatcta cagaaaaacg gaggtctggc agatagcagt aatgcaaaaa aatggagact
    23161 taagttgaat gaacgggagt aaagcgaaaa gactatagag tgaaggagaa attttgaggg
    23221 tgcgtctcac cgataaagat gagacgcgga aagattagta actggactgc tgggattttt
    23281 cagcctggat acgctggtag atctcttcac ggtgaacaga aacttccttc ggggcattta
    23341 cgccaatacg tacctggttg ccctttaccc ctaaaactgt cacggtgacc tcatccccaa
    23401 tcatgagggt ctcaccaact cgacgagtca gaatcagcat tctttgctcc ttgaaagatt
    23461 aaaagagtcg ggtctctctg tatcccggca ttatccatca tataacgcca aaaagtaagc
    23521 aacgacaaac acattacatg taagcagtca tggcattaca ttctgttaaa cctaagttta
    23581 gccgatatac acaacttcaa cctgacttta tcgttgtcga tagcgttaat gcgaatgccg
    23641 tgaagcgagt ccacggcatt gcctgacgct tatattattg caatttcgcg ctgacccagc
    23701 ctttcacact ggctaacgct gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca
    23761 tgtcaggacg tccaccaccc ttgccgccca cctgctgagc gaccatacca atcagttccc
    23821 ctgctttcac acgatctgtg acgtccttag atacgcctgc aatcagagaa accttacctt
    23881 cggctaccgt tgccagcacg ataattgtcg accccagctg attttttaaa tcgtcaacca
    23941 tggtacgcaa cattttcggc tcaacaccgc taagctcgct aaccaacagc ttaacaccat
    24001 taacatcaat tgccttactg gaaagatttg cgctctcctg tgcggcagct tgttctttaa
    24061 gctgttgtag ttctttttct aactgacgcg tacgttccag tactgagcgc actttatccg
    24121 ccagattatt gctatcgcct ttcagcagat gcgcgacttc gcttaagcga tcgctgtctg
    24181 catgaacggt ggtgatagcg ccttctccgg ttaccgcttc gatacgacga acgcctgcag
    24241 cagtacccga ttcagagatg atgcggaaca gaccaatatc accagtgcgg ctggcgtgag
    24301 taccgccaca cagctcggtg gagaaatcgc ccatgctcag cacgcgtaca cgctcatcat
    24361 acttctcgcc gaacagcgcc atcgcacctt tcgctttcgc cgcttcgaga ttcatgatgt
    24421 tggtttcgat cggcaaattg cgacgaatct gtgcgttcac caggtcttcg accgcacgaa
    24481 tctcttccgg tttcatcgct tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa
    24541 ccagtgaacc tttctgcgat acatgagtcc ccagaacctg gcgcagcgca gcgtgcatca
    24601 ggtgcgttgc ggagtgattc agacgaatac gggcgcgacg agcctcatca atatcagcct
    24661 gcacagcgtc gcccactttc agagaacccg cagcaagttt accgatgtga ccaatcgcct
    24721 ggccgtattt ctgcgtatct tccaccgcga aggagaagtt agcgcctttc agttcgcctt
    24781 tatcaccaac ctgaccgccg gattccgcat agaatggcgt ttgatccagc acgaccacag
    24841 cttcctggcc tgcattgatg gcatcaaccg ctttaccatc aacaaacagc gcggtcactt
    24901 tgccgttcag ttccagatgg tcatagcctt taaattcaga tgcactatca acacggatca
    24961 ttgcgttgta atcggcacca aagccgctgg cttcgcgcgc gcgacgacgc tgctcttcca
    25021 ttgctgcttc aaaaccagct tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg
    25081 tcaggtcaac cgggaagcca taggtgtcgt acagacggaa agccgtttca ccatccagcg
    25141 tatcaccaga aagttttgcc agctcttcat ccagcaacgc cagaccgcgc tccagagtac
    25201 gggcaaactg ctcttcttca gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca
    25261 ggtcttcacc cgcagagccc ataacgtcga tcagcggacc aaccagtttg tagaagaagg
    25321 tttctttcgc gccgagcata ttgccgtgac gcactgcgcg acgaatgata cgacgcagta
    25381 cataaccacg gttttcattg gacggcatta cgccatccgc gatcaggaac gcacaagaac
    25441 gaatgtggtc agcgattacg cgcagcgatt tattgctcag atcggtcgcg ccagtgactt
    25501 tcgctaccgc ctggatcaac gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt
    25561 gcagcaccgc agcaatacgc tccagaccca taccggtatc tacagacggc ttcggcagcg
    25621 gttccatcgt gccatctgcc tggcggttga actgcatgaa gacgatgttc cagatctcaa
    25681 tgtagcggtc gccgtcttct tccggacttc ccggagggcc accccaaatg tggtcgccgt
    25741 gatcgtagaa gatttcggtg cacgggccgc acggaccagt gtcacccatc tgccagaagt
    25801 tgtcagatgc gtatggcgca cctttgttat cgccgatgcg aataatacgt tcgcgcggga
    25861 tcccgacttc tttttcccag atttcgtagg cttcgtcgtc gctttcatag acggtaaccc
    25921 acagacgctc tttcggcagg gcaaaccatt tttcgctggt cagcagttcc catgcaaact
    25981 gaatggcatc gtgtttgaaa tagtcgccga agctgaagtt gcccagcatt tcgaagaagg
    26041 tatggtgacg cgcggtgtaa ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca
    26101 cgcagcgttg ggaagtggta gcgcgggaat aattacgctt gtcgagccca aggaacacat
    26161 ccttgaactg gttcatcccg gcgttggtaa acaacaaagt tgggtcgtta tggggtacca
    26221 gggagctgct ggcaactacc tgatgtccct tactatggaa aaagtcgaga aacgcctgac
    26281 ggatctcagc ggtgctcttg ctcataatta tcctgaaatc aagctaacga aatatcgcca
    26341 ccagctccag cgtgtcttaa ccgccgggct ggtaactgaa aagtgggaat aagataagtt
    26401 ttcttgactg ggaagtaaaa taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc
    26461 tggatatctt ccatcagata gccacgatag agcagaaaac gctggatctt aactttttct
    26521 gaaaagacag ttggcaaagg ttcaccatat tttcgcgtcg cctgatcgcg cgccagtgcg
    26581 caccagtcga tgtcacattc acgcatcgct ttttctgtcg cttcgcggga aatacctttc
    26641 tgattcagtt cctgacgaat acgtgcaggt ccataacctt tgcggctacg gctggcgata
    26701 aagcgcgcaa caaatcggct gtcatcgaga tagccatgtt catggcacca ggcaataacg
    26761 cgctcgtaat cttctgccgt agcatcaatc tcttctgggc catttttgcc cataatcggt
    26821 gccgcgagtt tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc
    26881 gcacgatcca acaggcgagc atatgccggg cgacgggatg ttgattctgt catggcatat
    26941 ccttacaact taaaaaagca aaagggccgc agatgcgacc caggttttgc tgataaacag
    27001 atttaatagt caatgagtat gcagaatgtt cccgcaggcc caattcgtta gaggatcgag
    27061 gtgagagtaa tttttcacgc cagtgctggc ctgttcccct caccctaacc ctctccccaa
    27121 aggggcgagg ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa
    27181 aatcttcgtt agtttctgct acgccttcgc tgtcatctac agagaaatcc ggcgttgagt
    27241 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
    27301 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
    27361 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
    27421 cgtagaagtt gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag
    27481 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
    27541 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
    27601 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
    27661 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
    27721 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
    27781 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
    27841 gggcgtcaca gatttccagt gcctgctcgc cggtatccgg ctgggagcac agcaggttat
    27901 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
    27961 acgcacaggt tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt
    28021 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
    28081 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
    28141 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
    28201 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
    28261 ataccggata gtcaatatgt tctgttgaaa caattatact gtatgctcat acagtatcaa
    28321 gtgttttgta gaaattgttg ccacaaggtc tgcaatacat acgcagtagc ctgacgacgc
    28381 accgcatcac ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg
    28441 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
    28501 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
    28561 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
    28621 atcatctgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag
    28681 ctaccggcaa tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt
    28741 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
    28801 tcactgtcag tcatcacaac ctctgtcagt caaaaattta tgccggacaa gatagcactt
    28861 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
    28921 tagccagagg gaagctcacg cccccctctt gtaaatagct actgtactcg cgccagcgcc
    28981 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt gtaacgggtg
    29041 atggtgtaga agttcggcag accgtaccag tactggtagc cggtgccaac atccagacgc
    29101 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgcggcggca
    29161 agctgcgaaa tgctgtactt agttttgaaa ccatttggca agcctggagc ctgaccgttt
    29221 gccattatcg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccacg
    29281 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
    29341 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
    29401 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcagcaggaa ggtttccagc
    29461 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
    29521 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
    29581 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
    29641 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
    29701 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
    29761 atcagccgca gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga
    29821 cgatcgaaac cgtgtttgtt caccattttg tcgatgaact gctgggcatt cgggttatta
    29881 gcgaaatcgc cgcccatctg catcacattg tgctgcggct caagcaggaa gccgccagac
    29941 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
    30001 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
    30061 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
    30121 ctgcccgata cggactttac ataactcaac tcattcccct cgctatactt ttactcaaac
    30181 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacactttt aaacctttca
    30241 acctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
    30301 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
    30361 aggtgtttac cggaatggtg accggcattc ttccgctgtt gattagcctg ctggttatca
    30421 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
    30481 ccggtaaccc tgtttcccgt tacctactgt taccgtgcat tggcacgttt gtcttttgca
    30541 atccgatgac cttaagccta ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
    30601 cggcggcctc ttatagctgc cactcaatga atggcctctt cccccatatc aaccctggcg
    30661 aactgtttgt ttatcttggc attgccagcg gtctgacaac gctgaacctg ccacttggcc
    30721 cactggcggt gagttatctg ctggttggtc tggtcaccaa tttcttccgc ggctgggtga
    30781 ccgatctgac caccgccatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc
    30841 acctggcagg agcaacatca tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg
    30901 cggcccgctt gagctggaag ctacgccggg caaaaaaatc gtctatatca ccgccggtac
    30961 ccggcctgcg attgttgaca aactggcaca acttactggc tggcaggcta ttgacggatt
    31021 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
    31081 attacgctgc ggcatctatc cgaaacgacg tattcccacc attaatatcc actcgacggg
    31141 caagtccggt ccgctggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
    31201 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
    31261 tgacaccagt aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
    31321 catggggtcc gccgttgccg tgttgtttca gtctggtcgt gacaccatcg acactgtatt
    31381 aaaaaccatt ctgccgttta tggcattcgt ctcggcgctc attggcatca ttatggcttc
    31441 tggccttggt gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
    31501 ggtcatgctg gcgctcatct gctccttccc actgctttca cctttcctcg gcccaggcgc
    31561 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
    31621 gccgcatctg gctttaccgg cactgtttgc catcaacgcg caggcggcct gcgacttcat
    31681 cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
    31741 tgtactggtg agccgctttt taaccggcgc gccgactgta ctgatcgcct ggtttgtctc
    31801 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
    31861 gtatcggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
    31921 gcgcgccagc ggacctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
    31981 cactccatcc cggtttgcaa ttttcactcg ggcagcatcg ctatccggtg accgctgttg
    32041 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
    32101 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
    32161 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
    32221 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
    32281 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
    32341 ttaacgccga atatggtgaa ggtatggcgt acggttttgg tgctgacgcc actagcgagc
    32401 aaagcgttct ggcgctctct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
    32461 tctatagcgc cggaatagca aaagcagcct ttatcagcga cttccagctc ggcgattttg
    32521 accgttcgct acaagtgaat ctggtgggtt atttcctgtg tgcccgtgaa ttttcgcgtt
    32581 tgatgatccg cgacgggatt cagggtcgca ttattcagat caactcgaaa tccggcaaag
    32641 tgggcagcaa acacaactct ggctacagcg cagcgaaatt tggtggcgtc gggctgactc
    32701 aatcactggc gctggatctg gcggagtacg gcattacggt gcattcactg atgctcggta
    32761 acctgctgaa atcgccgatg ttccagtcac tgttgccaca atacgcgacc aagctgggta
    32821 tcaaaccgga tcaagtcgag cagtattaca tcgacaaagt accgctcaaa cgcggctgcg
    32881 attatcaaga tgtgctgaat atgctgctgt tctacgccag tcctaaggcg tcgtactgca
    32941 ccggacagtc gatcaatgtc accggcggtc aggtgatgtt ctgatcaaca gcggagatcc
    33001 gttaaggatc tccgtgagac tatagaatgc ctgatgcgct acgctcatca ggcatacagg
    33061 acttccgcca ctacattaag gaaaagttat ggtatccgca ctcatcaccg tcgccgttat
    33121 cgcctggtgt gcgcaactgg ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt
    33181 cgacacacta tgccagcaag ggcgggttgg cgtgggccgt tccagcgggc actttaaacc
    33241 gcgggtcgtg gtcgccatcg cgctggacga tcagcagtgc atcgtcgaca ccttgtttat
    33301 gaaaggactg actgtcttcg cccgaccgca aaaaattccc gcaattaccg gcatgcatgc
    33361 gggtgattta cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc
    33421 attggcgctt aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt
    33481 atcattttga aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc
    33541 cattctggag tatctgcaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt
    33601 tgacaccaca ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg ccggaaccgt
    33661 cattcgtact tatggcggag tggtgttgaa taaagaggaa tccgatccgc ctatcgatca
    33721 taaaacgctc atcaacaccc acaagaaaga gctgattgca gaagctgccg ttagttttat
    33781 ccatgatggc gattcgatca ttcttgatgc tggcagtacc gttttgcaga tggttcccct
    33841 gctctcgcgc tttaataaca tcacggtgat gaccaacagc ctgcatatcg tcaatgcgct
    33901 atccgaactg gataacgaac aaactatcct gatgccaggc ggaacatttc gcaaaaaatc
    33961 ggcctcattt cacgggcaac tggcagagaa tgccttcgag catttcacct tcgataaatt
    34021 gtttatgggc accgacggca tcgatctcaa tgcgggcgta accacgttca acgaggttta
    34081 taccgtcagt aaggcaatgt gcaatgccgc gcgcgaagtg attttgatgg cggactcatc
    34141 aaagtttggc cgtaaaagcc ccaacgtagt ttgcagtctt gaaagcgtcg ataagctaat
    34201 taccgacgca ggtatcgatc cggcgtttcg tcaggcgctg gaagagaaag ggatcgatgt
    34261 gatcataacc ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa
    34321 tgctggagtt gcaggaagca agccgtttac cggaacgtct gggcgatgat tttgttcgcg
    34381 ccgccaatat catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg
    34441 gccacattgg taagaaaatc gccgcaacac ttgccagtac cggcacaccg gctttttttg
    34501 tccatccggc agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc
    34561 tgtttatctc ttactccggt ggcgcgaagg aactggatct gattattccg cgtctggaag
    34621 ataaatctat cgcgctgctg gcgatgaccg gcaaaccgac gtcaccgctg ggcctggcgg
    34681 cgaaagcggt gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga
    34741 cctccagcac cgtcaatacc ctgatgatgg gtgacgcgct ggcgatggcg gtcatgcagg
    34801 cgcgcggttt taatgaagaa gattttgccc gctcccaccc agctggggca ctgggcgctc
    34861 gcttgctgaa taaagtgcat catctgatgc gtcgtgatga cgctatccca caggtagcgt
    34921 taaccgccag cgtgatggat gcgatgctgg aactcagcag caccggtctg gggctggtgg
    34981 cggtatgtga cgctcaacaa caggtacaag gcgtctttac cgacggcgat ttacgtcgct
    35041 ggctggtagg cggcggcgca ctcaccacgc cagttaatga agcgatgacg acaggcggca
    35101 ccacattaca ggcacaaagt cgcgccatcg acgccaaaga agttctgatg aaacgcaaaa
    35161 tcactgccgc accagtggta gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg
    35221 atttctatca ggccgggatt atttaatcct tcaatcccag acgtttcgcc agccgatgca
    35281 ggttggcgac gtcagtttcc agcattcgcg cacaggctgc ccagttgtga tgattttgtg
    35341 ccagtgcctg gcgaatagtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct
    35401 taacaacggg caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat
    35461 gttgcgcctc aagaatcact tcatcgccgt tgcgggtggc tctcgccagc actaccgccc
    35521 gatgaatagc atgttccagt tcgcgcacgt ttcccggaaa actgtagtgt tgcagtaaat
    35581 ttcgcgcccc ggcacttaat accacgcggg agagcccctg ccgcaaacga cactgctcgc
    35641 agaaataccc cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcaccgaaa
    35701 gtggaaacac gctcaggcga tgaaacaaat cggcgcggaa tcgccctgcc agcacctctt
    35761 cgcgtaagtc gcggttagtc gccgccagca cgcgcacatc gacccgcaaa ctacggtcat
    35821 tgccaacgcg ctgaatatcg ccatactgca acaccctcag cagcttggcc tgcaatgcca
    35881 acgacaactc gccgatctca tcgagaaaca gagtaccgtt atccgccatt tcaaacttcc
    35941 cgctgcggtt actgatagcg ccagtaaacg ctcctttcac atgcccgaac aactcacttt
    36001 ccgccacact ttccggcagt gcggcacagt tgagatagac cagcggattc accgcccgtg
    36061 gcgaggcttc atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctga
    36121 tcagaacgtt gagatcggac gccgccacaa tctcaatctc ttttttcagt tgcgtcatgc
    36181 caggggacaa gccaatcatc tgcgtctgtt tcaccgcttc aaacggcgtg gcatcgcctg
    36241 gcagcatatt ctggctttcc agttgttcaa tcagcaacgc attgcttaac gctcccgccg
    36301 ccagcgcggc aatcagccgt aactcttcgt cgctgaaaac atcgaactga tcgggctgca
    36361 tcccgtcgag cgtcagtgcg ccgatcaggt tttgtccgac aaacaatggc agaccaacgc
    36421 aggcgtgaac cttcagactc tcctgcccag gaatcaaacc gtcataggga tcgggcaatt
    36481 cgctgtctgc gggaaagcgc accacatccc cggcgcgggc aatcgcttcc agccgtggat
    36541 gcccttccag cgcaaagcgt ctaccgagta catcctttgc cagaccgtcg atggcaagcg
    36601 gaataaactg ccgcgaatcg taacgtagca acgcagacgc atcgcactcc agcacctgac
    36661 gtagcgtggt gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga
    36721 tggcgatatt cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac
    36781 tatagatatt gtcatatcga ccatttgatt gatagtcatt ttgactactc attaatgagc
    36841 ataattttat ttatagagta aaaacaatca gataagaaac tggcacgcaa tctgcaatta
    36901 gcaagacatc tttttagaac acgctgaata aattgaggtt gctatgtcta ttgtggtgaa
    36961 aaataacatt cattgggtgg gtcaacgtga ctgggaagtg cgtgattttc acggcacgga
    37021 atataaaacg ctgcgcggca gcagctacaa cagctacctc atccgcgaag aaaaaaacgt
    37081 gctgatcgac accgtcgacc ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga
    37141 aatcgatctg gcggatatcg attacatcgt gattaaccat gcagaagagg accacgccgg
    37201 ggcgctgacc gaactgatgg cgcaaattcc cgatacgccg atctactgta ctgccaacgc
    37261 tatcgactcg ataaatggtc atcaccatca tccggagtgg aattttaatg tggtgaaaac
    37321 tggcgacacg ctggatatcg gcaacggcaa acagctcatt tttgtcgaaa caccaatgct
    37381 gcactggccg gacagcatga tgacttacct gacaggcgac gcggtgctgt tcagtaacga
    37441 tgctttcggt cagcactact gcgacgagca tctgttcaac gatgaagtgg atcagacgga
    37501 gcttttcgag cagtgccagc gttactacgc caatatcctg acgccgttca gccgcctggt
    37561 aacgccgaaa attaccgaga tcctgggctt taacttacca gtcgatatga tagcgacctc
    37621 ccacggcgtg gtctggcgcg ataacccgac gcaaattgtc gagctgtacc tgaagtgggc
    37681 cgctgattat caggaagaca gaatcaccat tttctacgac accatgtcga ataacacccg
    37741 catgatggct gacgctatcg cccagggaat tgcggaaacc gacccacgcg tggcggtgaa
    37801 aattttcaac gtcgcccgaa gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa
    37861 aggcgtgctg gtcggcactt cgacgatgaa taacgtgatg atgccgaaaa tcgccgggct
    37921 ggtggaggag atgaccggat tacgcttccg taacaaacgc gccagtgctt tcggatctca
    37981 cggctggagc ggcggtgcgg tggatcgcct ttccacgcgc ctgcaggatg cgggtttcga
    38041 aatgtcgctc agcctgaaag ccaaatggcg accagaccag gacgctctgg agttatgccg
    38101 tgaacacggt cgcgaaatcg cccgtcagtg ggcgctcgcg ccgctgccgc agagcacggt
    38161 gaatacggta gttaaagaag aaacctctgc cacgacgacg gctgacctcg gcccacgaat
    38221 gcagtgcagc gtctgccagt ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt
    38281 tgcgccagga acgccgtgga gtgaagtccc ggataacttc ctctgcccgg aatgctccct
    38341 cggcaaagac gtctttgacg aactggcatc ggaggcaaaa tgagcaacgg cattgtgatc
    38401 atcggttcgg gcttcgccgc ccgccaactg gtgaaaaata ttcgcaaaca ggacgccact
    38461 attccattaa ccctgattgc cgccgacagc atagatgagt acaacaaacc cgacctcagc
    38521 catgttatca gtcaggggca acgtgccgac gaccttaccc gccagacggc gggtgaattt
    38581 gccgggcagt ttaatctgcg cctgtttccg cacacctggg tgacggatat cgatgccgaa
    38641 gcccatgtgg tgaaaagcca gaataatcag tggcaatacg acaagttagt gctggcaacc
    38701 ggtgccagcg cctttgtccc gcctgtgcct gggcgtgagt taatgctgac gttaaatagt
    38761 cagcaagagt atcgcgcctg tgaaacgcaa ctgcgggatg cccgacgcgt gttgattgtc
    38821 ggcggtggtt tgattggtag cgaactggcg atggattttt gtcgtgcagg caaagcggtc
    38881 acgctaatcg acaacgctgc cagtattctg gcgtcattaa tgccaccaga agtaagcagc
    38941 cgcttgcagc atcggttgac ggagatgggc gttcatctgc tgttgaaatc tcagttgcag
    39001 gggctggaaa aaacggattc tggcattctg gcaacgctgg accaccagcg tagtatcgaa
    39061 gtggatgcgg taattgctgc caccggactg cgcccggaaa cagccctggc acgacgcgcc
    39121 gggctgacga ttaatcgcgg cgtttgcgtc gatagttatc tgcaaaccag taatgccgat
    39181 atttacgcgc tgggcgattg cgcggaaatt aacggtcagg tattgccgtt cctgcagccg
    39241 attcaactta gcgggatggt gctggcaaaa aatcttctcg gcaataacac gccgctgaaa
    39301 ctaccggcga tgctggtgaa aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa
    39361 acccagcgtc aggatttacg ctggcagatt aataccgaac gccagggaat ggtggcgcgc
    39421 ggcgttgacg atgctgacca gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag
    39481 gcatttgtat tgttgaaaac gttgccggtg taggtgggct actgtgccta aaatgtcgga
    39541 tgcgacgctg gcgcgtctta tccgacctac ggggacgcat gtgtaggccg gataaggcat
    39601 ttacgccgca tccggcaagg gtgtccaaat gcaacacgct ttatccgttc tggacttcac
    39661 ccgctaacca acgcgccgca gcaataaccc cctgccccag agacaaaccg ccatcacccg
    39721 ccggtaaact ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc agacgtgcac
    39781 gcagcaaacg gttatgaata accccgccgc taaataccag cgtagtgata ccacgcatcg
    39841 tggcctgctc acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg
    39901 cgcgttgatt aaccggtgcc tgccagttca gccactgctg ccagaaagtg gcgagatcca
    39961 gttgattgtc cacccgcggc atcgtcaccg gatgcgtcac tccgtcgcat gaggctgcga
    40021 gcgcctccag agcacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca
    40081 gtgccgccgc cactgcatcg aacaaccgcc cacacgatga tgccagcggc gcgttaattc
    40141 cacgctcaat ggcccgcgcc agtacgctcc agttttgctg ttgcacactt gccgtttcag
    40201 ggtaattctg ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct
    40261 gcttcgccgc caaatcgcca cccggaagcg ccactgcagg caagccgccc aggtgctcac
    40321 attcgcgata gttcacccgc aggcactcgc cgccccacaa agcgccattc tcccccatac
    40381 cgataccgtc gagcgtcaaa gcaatgacat caccgccatc cagcggccac tgatgctctg
    40441 ccagacacgc cgctgcatgg gcatgatgat gcagtaccgt ttgcgtcggc agattcattt
    40501 cgctcgccca ctggctggag acatagcccg gatgcacatc atgcacaatg tattgcggag
    40561 tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat
    40621 cgtcacttaa atcgcccaga tgctgactca acaccacttg ttcaccgcgc accaggcaga
    40681 aggtattttt cagatccgcg ccaagacaca gcacaggcgg aacattttta aagcccggag
    40741 gcaaaaccag cgcatccggc acataccccc gcgaacggcg cagcatttcg ccgctttcgc
    40801 gcaccactga atcatccatc cgctgcacga tgtcgcggtt atgtagcaag aatccgtcgg
    40861 caatgccctg caaatccgcc agcgcctgtt cgttgctgat agctggtggt ttaccgctca
    40921 ggttgccgga ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg
    40981 ggttcgcagg caacatcacc ccgacttcgt taaggccagg ggcgatatca tcacaaagct
    41041 caggtacgta ttttttatcc accagcacaa tcggcgcagc gggcgtggta agcaactggc
    41101 gcgcagcgtc tggtaaactt tctgccactg gcaacatgac tgccagcggt ttcgccgggc
    41161 gatgtttgcg ctcccgcagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat
    41221 gaaatccgcc aatccctttg atggcgacaa tgttgcccat ttttaactgt gcgatagctg
    41281 cctgtaatgc cgcctcttgt tctgcatgtt caccatgact tacccattca agatgcgggc
    41341 cacactccgg gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcgcggtact
    41401 ctttgtcaca ggccggacat agcggaaacg ccgccatcac ggtaaacggg cggtcgtaag
    41461 gcatcgcgcg aataatggtg aaacgcgggc cgcagtgggt acagttgata aacggataac
    41521 gataacgccg ctcgcctggg gtattcattt cggcaaggca agcagggcaa gtggcggcat
    41581 ccgggacaat ttgcgtattc atggtgccgc cggtgctctg gcggatggtg aactcggtgg
    41641 gcagttgtga ccagataaac ggctcacgct cgacgctatc aatacgcgcc agtggcggac
    41701 agtgctgatg caattgaaca agaaacgttt ccggatcttc cagcagccgg acttctacgc
    41761 catcgccgtc attacataca tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa
    41821 acggacgaaa accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaaaatg
    41881 tgttttttgc cattgagtta ttcccgccat catgaattgc gtaacccgcc ctgccggaca
    41941 cgacagcgtc gtatccggca gtctcaggtc ggcgataccg cccgctccgt attctacgaa
    42001 tatttccggg aattcctttg atgccagaac agttctgtaa gatttttaga acatcagcgc
    42061 cgtacggcgg cgtttttctg cgctcagttg ttcaagttta ttacgatcga cacaaatcag
    42121 cgcatgggtc gggcaagccg ccatacacgc cgggccgtct tcacgatggt tgcacaggtc
    42181 gcatttattg gcttcggctt tgtcagcccg tacattcaga cccgcgccgc tgttgcgaat
    42241 cacaggacgt accaccactt ccatcgcgcc atacgggcaa gccaccacgc aggttttgca
    42301 accaatgcaa cgttcctgca tcacatgaac aaacccttta tcacagctga tagcaccatt
    42361 cgggcagacg ttagcgcacg gtgcatcttc acactgacgg caaactgtcg ccgtggaaat
    42421 gttcacacct ttaatgacat ggatacgcgg taaaaatgtt tccggggtca gcgatgcaca
    42481 gtcctgattt tcctgatgag aaaccacgca cgccacttca caggtacggc aaccaataca
    42541 tttactcgcg tcagcaatga tgaaacggtt catcaaattc tccagcaatg acagttaatg
    42601 c
//
LOCUS       DAJTYF010000020        41031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTYF010000020 DAJTYF010000000
VERSION     DAJTYF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.020"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..221)
                     /locus_tag="OO590_001225"
     CDS             complement(<1..221)
                     /locus_tag="OO590_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HCR6636663.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIG"
     gene            complement(261..1106)
                     /gene="glpF"
                     /locus_tag="OO590_001226"
                     /pseudo
     CDS             complement(261..1106)
                     /gene="glpF"
                     /locus_tag="OO590_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            1532..1777
                     /gene="zapB"
                     /locus_tag="OO590_001227"
     CDS             1532..1777
                     /gene="zapB"
                     /locus_tag="OO590_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6636664.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            complement(1862..2347)
                     /gene="rraA"
                     /locus_tag="OO590_001228"
     CDS             complement(1862..2347)
                     /gene="rraA"
                     /locus_tag="OO590_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6636665.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(2440..3366)
                     /gene="menA"
                     /locus_tag="OO590_001229"
     CDS             complement(2440..3366)
                     /gene="menA"
                     /locus_tag="OO590_001229"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6636666.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(3433..4764)
                     /gene="hslU"
                     /locus_tag="OO590_001230"
     CDS             complement(3433..4764)
                     /gene="hslU"
                     /locus_tag="OO590_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6636667.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(4774..5304)
                     /gene="hslV"
                     /locus_tag="OO590_001231"
     CDS             complement(4774..5304)
                     /gene="hslV"
                     /locus_tag="OO590_001231"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6636668.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(5397..6356)
                     /gene="ftsN"
                     /locus_tag="OO590_001232"
     CDS             complement(5397..6356)
                     /gene="ftsN"
                     /locus_tag="OO590_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6636669.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            complement(6448..7473)
                     /gene="cytR"
                     /locus_tag="OO590_001233"
     CDS             complement(6448..7473)
                     /gene="cytR"
                     /locus_tag="OO590_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6636670.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(7629..9827)
                     /gene="priA"
                     /locus_tag="OO590_001234"
     CDS             complement(7629..9827)
                     /gene="priA"
                     /locus_tag="OO590_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6636671.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            10030..10242
                     /gene="rpmE"
                     /locus_tag="OO590_001235"
     CDS             10030..10242
                     /gene="rpmE"
                     /locus_tag="OO590_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6636672.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(10303..10911)
                     /locus_tag="OO590_001236"
     CDS             complement(10303..10911)
                     /locus_tag="OO590_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6636673.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(11095..11412)
                     /gene="metJ"
                     /locus_tag="OO590_001237"
     CDS             complement(11095..11412)
                     /gene="metJ"
                     /locus_tag="OO590_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6636674.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            11689..12849
                     /gene="metB"
                     /locus_tag="OO590_001238"
     CDS             11689..12849
                     /gene="metB"
                     /locus_tag="OO590_001238"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6636675.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            12852..15284
                     /gene="metL"
                     /locus_tag="OO590_001239"
     CDS             12852..15284
                     /gene="metL"
                     /locus_tag="OO590_001239"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6636676.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            15633..16523
                     /gene="metF"
                     /locus_tag="OO590_001240"
     CDS             15633..16523
                     /gene="metF"
                     /locus_tag="OO590_001240"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6636677.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            16852..19032
                     /gene="katG"
                     /locus_tag="OO590_001241"
     CDS             16852..19032
                     /gene="katG"
                     /locus_tag="OO590_001241"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6636678.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALLVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            19126..20031
                     /gene="yijE"
                     /locus_tag="OO590_001242"
     CDS             19126..20031
                     /gene="yijE"
                     /locus_tag="OO590_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6636679.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(20058..20675)
                     /locus_tag="OO590_001243"
     CDS             complement(20058..20675)
                     /locus_tag="OO590_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR6636680.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20950..22042)
                     /gene="gldA"
                     /locus_tag="OO590_001244"
                     /pseudo
     CDS             complement(20950..22042)
                     /gene="gldA"
                     /locus_tag="OO590_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(22053..22715)
                     /gene="fsa"
                     /locus_tag="OO590_001245"
     CDS             complement(22053..22715)
                     /gene="fsa"
                     /locus_tag="OO590_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6636681.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPCQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(22727..25228)
                     /gene="ptsP"
                     /locus_tag="OO590_001246"
     CDS             complement(22727..25228)
                     /gene="ptsP"
                     /locus_tag="OO590_001246"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6636682.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRVRTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            25537..26616
                     /locus_tag="OO590_001247"
     CDS             25537..26616
                     /locus_tag="OO590_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6636683.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            26631..26951
                     /locus_tag="OO590_001248"
     CDS             26631..26951
                     /locus_tag="OO590_001248"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6636684.1"
                     /translation="MTKIIAVTACPSGVAHTYMVAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            27002..29299
                     /locus_tag="OO590_001249"
     CDS             27002..29299
                     /locus_tag="OO590_001249"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6636685.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            29265..30143
                     /locus_tag="OO590_001250"
     CDS             29265..30143
                     /locus_tag="OO590_001250"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6636686.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            30145..30486
                     /locus_tag="OO590_001251"
     CDS             30145..30486
                     /locus_tag="OO590_001251"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6636687.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(30473..31324)
                     /locus_tag="OO590_001252"
     CDS             complement(30473..31324)
                     /locus_tag="OO590_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6636688.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(31473..33206)
                     /locus_tag="OO590_001253"
     CDS             complement(31473..33206)
                     /locus_tag="OO590_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6636689.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(33389..36040)
                     /gene="ppc"
                     /locus_tag="OO590_001254"
     CDS             complement(33389..36040)
                     /gene="ppc"
                     /locus_tag="OO590_001254"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6636690.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLDYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(36392..37543)
                     /gene="argE"
                     /locus_tag="OO590_001255"
     CDS             complement(36392..37543)
                     /gene="argE"
                     /locus_tag="OO590_001255"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6636691.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            37697..38701
                     /gene="argC"
                     /locus_tag="OO590_001256"
     CDS             37697..38701
                     /gene="argC"
                     /locus_tag="OO590_001256"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6636692.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            38709..39485
                     /gene="argB"
                     /locus_tag="OO590_001257"
     CDS             38709..39485
                     /gene="argB"
                     /locus_tag="OO590_001257"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6636693.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            39546..40919
                     /gene="argH"
                     /locus_tag="OO590_001258"
     CDS             39546..40919
                     /gene="argH"
                     /locus_tag="OO590_001258"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6636694.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         9342 a        10531 c        11258 g         9900 t
ORIGIN      
        1 ataccgatag ctgcaatttg atcggaactg atatcggctt tcgccagcac ttctaccagc
       61 gtggagcttt gggtggccca gatttccatt gggtcgtgtt ctacccaacc tggttttggg
      121 tagatttgct caaattcgcg ctgcgacacg ctaatgatat tggcatcgtg atccattacg
      181 accgcgcggg agctggtggt gccctggtcg agcgcaacga tatatttttt tcagtcatgt
      241 ttaattgtcc cgtagtcata ttacagcgaa gctttttgtt ctgaaggagt tgtggtttcc
      301 ttttcttcca caacacagat atcgcaaggc aaatggcgac caatcagttt gcggtaggca
      361 aatgcaccta caatcgcgcc aacgataggg ccgaaaagcg gcaccaggaa gtaaggaatg
      421 tctctgccgc cggtaaaggc gacattgccc cagcccgcca gctaggcaaa gactttcgga
      481 ccgaagtcac gcgctgggtt catggcaaaa cctgtcaatg ggcccataga tgcgccaatg
      541 accgcaatca gtagaccaat cagcaaggga gccaaagggc cgcgtggtac accgttgcca
      601 tcgtccgtta acgccaggat aagccccatc agaatagcgg taatcaccat ctcaactgcg
      661 aaagcctgca caaaattgat atgaggatta gggtaagtag agaaagtgcc agccagatca
      721 acactttcaa cgctgccgcg aacaatgtga tgagtctgct cgaagtcgaa aaataaattg
      781 tagtaaagcc cgtaaactaa agccgcagcg cagaaagcgc cggcaacttg tgaaacgata
      841 aaaggaataa ctttgcgctt gtcgaaacag gcaaacagcc acaatgcaat ggtaacagcg
      901 ggattaagat gcgcgccgga aacccctgcg gtcaggtaga tggccattgc cacccccagt
      961 ccccaaatga cactgatttc ccactgacca aaagacgcac cagcgacttt tagtgctgca
     1021 acgcaaccca caccgaaaaa aatcaacaac ccggtaccga ggaattcagc aatgcactgg
     1081 cctttcaagg ttgatgtttg actcataatc ggatcctgaa gagttaatgt ttgttgtatg
     1141 tgtgaaagtc acggacctcc acgatgcttg taggcatgct gttaacttat cgttaacgag
     1201 caaaaacgag aaatatcgaa cttaaaatgt gtgtgcttcg tcataaaatg agcgttatcg
     1261 cgccatttat tccatctgat tgtacatgat gcatgtttgt tagatcattc gcgcaacaaa
     1321 tttattaaca ttttcgattg atgtggatta tgtcactagg agaaggatga aaagtgtggc
     1381 aaaccgtaat ctgcaaggcg tgccgctgga cagcgatggc gcggcttcat acaatcggag
     1441 ctaactaaag tgcgctcgta tttattaagg cgtcaccggt aatcgggacg aggatttttt
     1501 atccatcaac gccttgcaat tcaggagagg tatgacaatg tcattagaag tgtttgagaa
     1561 actggaagca aaagtacagc aggcgattga taccatcact ctgttgcaga tggaaatcga
     1621 agagctgaaa gaaaaaaaca actcactgtc gcaggaagtt caaaatgccc agcatcagcg
     1681 cgaagagctg gagcgtgaga acaaccatct gaaagaacag cagaacggct ggcaggaacg
     1741 tctgcaggcc ctgctgggtc gcatggaaga ggtctgatta cgcttcatct ttcgcgtaat
     1801 ttctttactg tgtaggccaa caggtaacgc agaaaaaagg caccttgcgg tgcctttctt
     1861 atcattcaat atccagcgga tcttctgaaa gaataatccc ggtattgtcg gcataaagat
     1921 ggtcgccgga gaagaaggtg acaccgccaa aattgacgcg gacatcgctt tcgccaatgc
     1981 cttcgccagc ggcaccaacc ggaattgccg ccatcgcctg gatgccgata tccaactctt
     2041 ccaggtcatc tacctgacgc accgcgccgt aaatgaccag accttcccat tcattttgta
     2101 ctgccagacg cgccagttca gcatcgacca gtgcgcgacg aacagaacca ccgccatcga
     2161 cgacaagaac acgaccacgg ccattctgtt cgagcagatc gtacagcaac ccgttgtcct
     2221 cgaaacattt taccgtgatt atttgtccgc caaacgacgc ccgtccgcca aagttggaga
     2281 acagcggttc cacgacgtta acatcttctt gatagatgtc acaaagctcg gaagtatcgt
     2341 atttcatagg cttaacattc agttgctgcg agaattttca gtatatcgcg ctatgtgggc
     2401 tgttggcaaa gtcatcaatt gttaattgat atttgtcagt tatgctgccc actggcttag
     2461 gaatatccct aaaacaaaca gcaggttagt cagtaacgct cccttgacag tacgttccag
     2521 cattggtcgc atcgccaccg ggtccatttc ccgcatcaca taacgggctt gcttcaccag
     2581 taatggtgcc gccagcagga acagccagcc ccacaggcta tgcagcgaaa agagattaaa
     2641 cagcgccaga cacaccagcg agcccatcag caggcaggca tgataacgac gcgcgttcac
     2701 ttcacctaag cgcaccacca gcgtgttttt gccattttcg cggtcgctat tgatatcacg
     2761 caggttatta atattcagta ctgccgttgc cagcaggccg catgcggtcg ccggaaggat
     2821 cagtgccgga atcaatgtat gagcctgtaa ataccagctc cccatgacac tcaaccagcc
     2881 aaagaaaacc agtacggaaa tatcacccag accgatataa ccataaggac gattgcccac
     2941 ggtgtaggtg atagcggcaa tgatcgacaa cccgccaaga atcaggaaac cgacaaaatc
     3001 ggccagcgta tggcatgcca ctgcaaccag tgccagcccg gagagacaga tgagcacgac
     3061 ggtaataatg agcgcccgtt tcatcttttg ctgggtaatg accccttttt gcatgccgcg
     3121 tagcggccca atgcggtcag gtttatcgct gccttttacc gcatcgccgt aatcattggc
     3181 gaggttagaa aggatctgta atagcccggc ggtaattagt gccagcaggg cgaccagcgg
     3241 atcgaagtga ccttgccacc atgccagcgc cgtcccgacg ataattgcag caaaggcgag
     3301 ggggagggtt ttaggtcgta aactttccag ccacgcctga gttcggctaa tttgttgttc
     3361 agtcataata cgcgccaata aaaatggggc ctttcagccc catcaaacaa tgatgaaaat
     3421 gattgaacgc gattatagga taaaacggct cagatcttca tctgccacca acgcatccag
     3481 atgtttgctc acataatctg cgtcaatagt gatattttga ccgcttaaat cgctggcgtc
     3541 gtaggaaatc tcttccatta aacgctccag aacagtgtgt aaacgacgag caccgatgtt
     3601 ttcggtagat tcgttcacct gccatgccgc ttccgcgatg cgtttaatac cggagtcggt
     3661 aaactcgata tttacgcctt cagtcgccat cagtgctttg tactgcacgg tgatagaggc
     3721 attcggctcg gtcagaatac gctcgaagtc gctggtggtc agtgcctgca gttcaacgcg
     3781 gattggcagc cgaccttgca gttccgggat caggtcagac ggtttcgcaa tctggaacgc
     3841 gccagaagcg ataaacagaa tgtggtcagt tttgaccatc ccgtgtttgg tggaaacggt
     3901 gcaaccttct accagcggca gcaggtcacg ctgaacgcct tcacgagaaa catccggacc
     3961 ggaagactcg ccgcgcttac agattttgtc gatttcgtcg ataaacacga tcccgtgctg
     4021 ctcaacagcg tcgatagcgt cttgcttcag ctcttccggg ttcaccagtt tcgccgcttc
     4081 ttcttcaatc agcagcttca tggcgtcttt gattttcagc ttacgcgctt tttgcttctg
     4141 gccgcccagg ttctggaaca tggactgcag ctggctggtc atctcttcca tgcccggagg
     4201 agccataatt tcaacgccca tcggtgctgc ggcaagatcg atctcgattt ctttgtcatc
     4261 aagctggcct tcacgcagtt ttttgcggaa tgcctgacga gcagcggaag gttcctgctg
     4321 ctgttcagtc tgtccccagt tgtttttagc aggtgggatc agcacgtcga gaatacgttc
     4381 ttctgccagt tcttcagcgc gataacggtt tttctcgata gcctggacgc gtaccatttt
     4441 cacggcggca tcggtcagat cgcgaataat agaatcaact tccttaccga cgtagcccac
     4501 ttcggtaaat ttggtcgctt caactttgat gaacggcgca ttcgccagct tagccagacg
     4561 acgggcgatt tcagttttac cgacaccggt cgggccgatc atcaggatat ttttcggggt
     4621 cacttcatgg cgcagctctt cgttgagctg catgcgacgc cagcggttac gcagagcaat
     4681 cgccacagaa cgcttggcgt tgtcctggcc gatgatgtgc ttatccagtt cgctgacgat
     4741 ttcgcgtggg gtcatttcag acatgggaga tccttacgct ttgtagctta attcttcgat
     4801 ggtgtggaaa tggttggtat agatgcaaat gtcgcctgca atatccaacg ccttttcagc
     4861 aatttcacgg gcgctaagtt cagtgttttc taacagcgcg cgcgccgcag cctgggcgta
     4921 agggccgccg gagccgatag caataagatc gttttctggc tgcaccacgt caccgttacc
     4981 ggtgatgata agcgatgcag tttcatccgc gactgccagc agtgcttcaa gtttgcgcag
     5041 catgcgatcg gtacgccagt cttttgccag ctcaacggcg gctttaacca gatggccctg
     5101 atgcatttcc agtttacgtt caaacagttc gaacagcgta aaagcatccg cagtaccgcc
     5161 cgcaaagccc gcgatgactt tgtcgttgta cagacggcgg acctttttca cgttgccttt
     5221 cattacggta ttgcccaacg tggcctgacc atcaccagcg atgaccacat ggccgttacg
     5281 gcgtacgctt actatagttg tcacgagctg accccttggt tacgaataca gagtacaaac
     5341 cccgtacaaa agtacggggc ataatgcaat tatagatggg ggggattttg agggtttcaa
     5401 cccccggcgg cgagccgaat gcagtttgta tgacccgcca tcttcaaccg attgagggtg
     5461 ctgtctgcgt tctctttgcc tttcaccggg ccaatgacca cacgattcca gccattgttg
     5521 gtggtgattt tcgagtcaaa tccttcgaac gccagctgag cacgtaccgt ctctgcctgt
     5581 tccgcgcctc tgaacgaacc gcactgcacc atccagcggc gttcgtcttt tttctccgcc
     5641 gtcggttttg gcgcgtcagc ggcacgagca accggcgcag cttgctgcgg cttcgactgc
     5701 gcggtcgtgt gcgcaggggt ttgcagcaga tcctggtacg gctgctgagt agaagctggt
     5761 ttagactggc gcggttgcgc ctgtaccggc gcggcttgcg acgtacgcgt ctgctgctgc
     5821 cagctttgtt cagtcgtacg cgactgctga gccaggcgtt gctgctccgc taattgctgc
     5881 gcctgacgct ggcgttgtag cgtttgctga cgctgttccg gcgtctgttc attccacgga
     5941 acctcaacca gctgcgttgg ctgctggcgc atatcagcct gcatttgttc aagaagctga
     6001 cgttgttctg gtgtcagttg ctccggcgtt ttcacttcac caccggcaga aggttctgtg
     6061 ggcgcacgca cacccggctg gcgactttcc agctctttaa tgtagcgcca gcgttcttct
     6121 ggttttggtg gtagtccgtt tccggtcact ttctggcttt gcagcgtctc ggactcttct
     6181 ttcttgtgat gcgtaatgaa gtacagacca ccgataaagg tcacaagaac ggcggcagca
     6241 atagcgacca tagcgggaga aaccgcaggc agatttcgtt gctttttccg tgaggtgctc
     6301 ttttttcgcc gcgaaggtgc cggttggctg cggcgtacat aatctcgttg tgccactatc
     6361 gtttcgctgt atttattcgt tcgtcagccc gccatgttac ttaagcggcg ggcctttgac
     6421 cagtcacggc agtcttaaag gtttacttta aggtaacgcg cgtgttgatc cccggatgat
     6481 aagttcgcag tccattaaac gagagccact gccaacgtgt tgcccctgca tttgatcaag
     6541 caataacagc atagcttccc gaccgatttc gtaacgcggc tgcgcgatgg ttgtcagcgg
     6601 cggatcacaa aattgcgtca ggtcgatgtt atcaaaaccg attatggaaa ggtcttccgg
     6661 gactttcagc ccctggcgtt ttgcctgaga aagtgcgccg agcgccatca catcgctatg
     6721 gcagaagact gcagtaggcg gttgtggaag atcaagcagc tgttgcattg ctttgcttcc
     6781 ggcttcgaag gtgaagtcgc cacgggcgat gtattgtgga tcaaccataa ttccgcagcg
     6841 acgcagcgcc tgaacatagc cttgcaggcg gtagtgacac agtggcatct cttcgggacc
     6901 ggctatacag ccaatccgtt tatgcccttg ctcatataaa taatttactg catcaaatgc
     6961 ggcggtcaga ttgtcgatat gaacggtagg cagctccagt tccggtgcaa attcgttcgc
     7021 catcaccatc ggcggcagat tacgctgttc ctcaatgctg gcatcaaacg gcagccttga
     7081 acccagcagc aacatgccat caatttgctt ggtgatgatc aaatcgataa aggttttttc
     7141 ctgctgattt tgatgcgcac agtcgccaat cagcaccaga tatccgtgat ttgccgccgt
     7201 aacttcgata ccgcgaataa tttcgctaaa gaagggatcg cagatatccg ggacaatcac
     7261 cagaatggtg cgggattcat tacgcttgac gttgcgcccc ataggctgcg gtaaataacc
     7321 cacttcccgg gccgcttttt caacccgatt acgggtggcc tgggagactt tatcgggatt
     7381 cattaatgct cgggagacag tcgctgtaga gacttttgcc ttgagggcaa cgtctttcat
     7441 ggtcgcggca gtttcctgct tcttcgcttt cacactcact cctcgcctgg cacgtcaggc
     7501 gtactacatc catgtttact tcacatcggc aacattttta gcagatagcg cgtgaaaacg
     7561 gttacagaat tttcatgaaa agtgtgatga atattgaatt tttcgatccg cctcgcatcg
     7621 taagcggttt aaccctcaat cggatcaaca tccagtaccc atttcacctt gcgggaatcc
     7681 ggtattgtat tgatgagcgc cagcgtacca ctaatgatgt gttgcaagcg cacgcgggaa
     7741 gggtgctgca acaatatctg ccagcgccag cggccgccac gtttaggtgc cagtgccgga
     7801 accggaccga gaacccacag tttgtcgtct gccagcggac tggagaggat cagattacgc
     7861 agttgttgca ggaacagcgg cgcatgctga ttgttatgat cttccgcacg cacaatcaca
     7921 tggctggtcc atggcggcag ttgcatcatc cgtcgctcag ccagcgcctg ttcggcaaag
     7981 gcgtcgtagc ctttatagag caacgtttgc aacagaggat gttccggatg gtgcgtttgc
     8041 agcaccactt cgccttgttt acccgcacgc ccggcacgac cagcgacctg ggtgtaaagc
     8101 tgagcgaaac gctctgccga gcgaaaatcg gcagaaaaca gcgcgccgtc cacatccagt
     8161 aacgccacca gcgtcacatc cggaaagtgg tgacctttcg ccagcatttg tgtgccgatc
     8221 aaaatcctcg caccgccgcg atgtacttcc gccagttgct gttccagcgc ccctttgcgg
     8281 ctggtggtat cgcggtcgat acgagaaatg ggcacatcag ggaacagcgg cgcaagcgtc
     8341 tgttcaagct gttcggtgcc cagccccacg ggtaccaggt gcgtggaacc gcaggaaggg
     8401 cactggcgcg gcaccggacg ctgactgtca cagtggtgac agcgcaaatg ctgttgcgcc
     8461 tgatgcagcg tgtagtagtg atcgcaacgt gggcattcgg caatccagcc gcagtcgtgg
     8521 cacagtagcg caggcgcaaa gccacggcgg ttaagaaaga ggataacctg attgttggcc
     8581 tgtaaatgct ggcgcatacg ggtgattaac gccggagcca gacctgcctg caccttctga
     8641 ccttttaaat ccagcacgtg ttgaattgcc ggacgcgcat tccccgcccg gcgcgtcagg
     8701 cgcagcaggc ggtatttttt ctgctggacg ttgcacaacg tttccagcgc gggcgttgcg
     8761 gagccaagaa taatcgggat ttgctcgcta tgcgcacgat acaccgccag gtcgcgggca
     8821 tgatagcgcc agccttcctg ctgcttgtag gaactgtcgt gctcttcgtc aatgacaatc
     8881 acgccgagat ttttaaacgg cgtaaacagc gcggagcggg tgccgatcac aatcgccgcc
     8941 tcgccatttt tcgctttcag ccacgccgaa agacgctcgc tgtcgttcag accggaatgc
     9001 agaacttcca cgggcgcatt aaaccgttca cgaaaacggg cgatagtttg cggtgtcagg
     9061 ccgatttccg gcaccatcac cagcgcctgt ttgccctgag cgagcacgtt ttccagtaca
     9121 ctgagataaa cctccgtttt accggaaccg gtaacgcccg ccagcagcca ggcagaaaaa
     9181 gtatctgccg cgctatgaat tgcgccaacg gcggtggcct gttcggtatt caatcgcaac
     9241 cgctcaccag aaacggcata gttcgttcgc cagtcgctaa actctggtgt ttcacttgct
     9301 aaatcacaca gacctttttt gcgcagcgcc tgcaacgcgg catcattaaa ttcgagcgtg
     9361 gcaacctggt cgcgccagat tttgccttgc cgtaacgctg ccagcgcctg ttgttgcttg
     9421 ggtgagcgtt tcaggctgtt cagatccacc gcctgacctt gttcagtggc aaaccagtac
     9481 cacatcggcg cgttcgccgc aggccgcccc tggcgtagta aaatcggcaa ggcatgaaac
     9541 agcacatcgc caatcggatg atgatagtaa tctgccgccc atagcagcaa tcgccagacg
     9601 gagtgagtaa acaccggctc accatccagc acttcgacta ccgcttttag ctcagtgagc
     9661 ggcagttcgc tggcatcgct aactgatacc acaatcccga tgcgctcctg ctgtttgcca
     9721 aacggcacgc gcacgcgaca cccagcttta accgtcatgc cttctggcag cagatagtca
     9781 aaggtacgag gaagcggaac gggcaaggca acgtgggcaa cgggcatagc atcatcctga
     9841 cttgaaattc ggtgggttag tatacacatt gccgtagaag agtgcggatc agtttgcata
     9901 cgctggttaa tttctgtatg atttcgcgcc ttcgtacgaa atgatcgtat tgaagctata
     9961 cttttaacat cgcgtggtgt ctggcgttag ggctggaaga gcgacgcggc cttaaaccga
    10021 ggttttccca tgaaaaaaga tattcacccg aaatacgaag aaattactgc tagctgctct
    10081 tgcggtaacg taatgaaaat ccgctccacc gttggtcatg acctgaacct cgacgtgtgc
    10141 agcaagtgcc acccgttctt cactggcaaa cagcgtgatg ttgctaccgg tggccgtgtt
    10201 gaccgcttca acaagcgttt caacatcccg ggcagcaaat aagaccggat ttccgaaaaa
    10261 agcgccgcac ggcgcttttt ttgtgcctga attttggtcg tatcaccacg aaaacagcgg
    10321 ccactctttg gcaaccgtgg tgagttgtgg cgcgtcgaaa acggcttgtg gtgaaactac
    10381 cgtttcctca agcggaatat ttttaccgta gcgttctttg agcttcgcct ggaccagcgg
    10441 gttgctgaga tcaaactctt tcaacttctg ctgttcgccc acgcgcattc ccagcgcgtt
    10501 ctggtaaacc ttccacacca cttccgaaca gtactggcga tcgtcgctcc atgagaagct
    10561 gaaatcatac ggtttaccga gataacgttt tgccgtttgc gtcagttttt gctgttgttc
    10621 aacactcagt ccgccttcaa cgcggcgaac aacgtagttg cccttttcac catgggcaat
    10681 ccactgcttg agcggggtgt atttcaccgg gccgaccgct tcaaaaacgt agggcttttt
    10741 gttacgcatc accagcatac cggtgtggct gtaatcggaa tgggttgcca gttggatcgc
    10801 tttactttgc gatgagcgag agatttgaaa gatgatgtcg ccagtttgcg gctgccaggc
    10861 aaaggcaggt acagaaacca gcaggctgag gatcagcagc ctgtttttca tagttaaacg
    10921 tccatgtata aaaagcggtg ggtcgcagac aacgtgctcg ttgtttatgc cggatgcggc
    10981 gtgaacgcct tatccggcct acaagttcgt gcaaattcaa taaattgcaa tatgacgtag
    11041 gcctgataag cgtagtgcat caggcgattc cactccgcgc cgctcttttc cgctttagta
    11101 ttcccacgtc tccgggttaa tccccatctc acgcatgatc tcttttgccg cttccgggat
    11161 ttcgtcgctg cgctctttac gcagatcggc atcatccggc aaaggttgcc cggtaaaggc
    11221 atgcagaaac gcttcgcaca gcagctcgct gttggtagcg tgacgcaggt tgttcacctg
    11281 acgacgcgtg cgttcatcgg tgaggatttt taacacctta agaggaatgg aaaccgtaat
    11341 ctttttgact tgttcactct tcttgccgtg ttcagcgtat gggctgatat attcgccgct
    11401 ccattcagcc atgagatact taatcctctt cgtcaataaa ttgagaccag accccacaat
    11461 ttatgtgggt actgaccgta aacccgcata gtttaccgta caggcgttac cgtgacatcg
    11521 tgtaatgcac ctgtcggcgt gataatgcat ataattttaa cggctatttg ggatttgctc
    11581 aatctatacg caaagaagtt tagatgtcca gatgtattga cgtccattaa cacaatgttt
    11641 actctggtgc ctgacatttc accgacaaag cccaggaaac ttcatcacat gacgcgtaaa
    11701 caggccacca tcgcagtgcg tagcgggtta aatgacgacg aacagtatgg ttgcgttgtc
    11761 cctcccatcc atctttccag cacctataac tttaccggat ttaatgaacc gcgcgcgcat
    11821 gattactcgc gtcgcggcaa cccaacgcgc gatgtggttc agcgtgcgct ggcagaactg
    11881 gaaggcggtg ctggcgcagt acttactaat accggcatgt ccgcgattca cctggtaacg
    11941 accgtctttt tgaaacctgg cgatctgctg gttgcgccgc acgactgcta cggcggtagc
    12001 tatcgcctgt tcgacagtct ggcgaaacgc ggttgctatc gcgtgttgtt tgttgatcaa
    12061 ggcgatgaac aggcattacg ggcagcgctg gcagaaaaac ccaaactggt actggtagaa
    12121 agcccaagta atccattgtt acgcgtcgtg gatattgcga aaatctgcca tctggcaagg
    12181 gaagtcgggg cggtgagcgt ggtggataac accttcttaa gcccggcatt acaaaatccg
    12241 ctggcattgg gtgccgatct ggtgttgcat tcatgtacga aatatctgaa cggtcactct
    12301 gacgtggtgg ctggcgtggt gattgctaaa gacccggacg ttgtcactga acttgcctgg
    12361 tgggcaaaca atattggcgt gacgggcggc gcgtttgaca gctatctgct gctacgcggg
    12421 ttgcgaacgc tggtgccgcg tatggagctg gcgcagcgca acgcgcaggc gattgtgaaa
    12481 tatctgcaaa cccagccgtt ggtgaaaaaa ctgtatcacc cgtcgctgcc ggaaaatcag
    12541 gggcatgaaa ttgccgcgcg tcagcaaaaa ggctttggcg caatgttgag ttttgaactg
    12601 gatggcgatg agcagacgct gtgtcgtttc ctgggcgggc tgtcgttgtt tacgctggcg
    12661 gaatcattag ggggagtgga aagtttaatc tctcacgccg caaccatgac acatgcaggc
    12721 atggcaccag aagcgcgtgc tgccgccggg atctccgaga cgctgctgcg tatctccacc
    12781 ggtattgaag atggcgaaga tttaattgcc gacctggaaa atggcttccg ggctgcaaac
    12841 aaggggtaaa aatgagtgtg attgcgcagg caggggcgaa aggtcgtcag ctgcataaat
    12901 ttggtggcag tagtctggct gatgtgaagt gttatttgcg tgtcgcgggc attatggcgg
    12961 agttctctca gcctgacgat atgatggtgg tttccgccgc cggtagcacc actaaccagt
    13021 tgattaactg gttgaaacta agccagaccg atcgtctctc tgcgcatcag gttcaacaaa
    13081 cgctgcgtcg ctatcagtgc gatctgatta gcggtctgct acccgctgaa gaagccgata
    13141 gcctcattag cgcttttgtc agcgatcttg agcgcctggc ggcgttgctc gacagtggta
    13201 ttaacgatgc ggtgtatgcg gaagtggtgg gccacggtga agtgtggtcg gcgcgcctga
    13261 tgtctgcggt acttaatcaa caagggctgc cagcggcctg gcttgatgcc cgtgagtttt
    13321 tacgcgctga acgcgctgcg caaccgcagg ttgatgaagg gctttcttac ccgttgctgc
    13381 aacagctact ggtacaacat ccgagcaaac gtctggtggt gaccgggttt atcagccgta
    13441 acaacgccgg tgaaacggtg ttgctggggc gtaacggttc cgactattcc gcgacacaaa
    13501 tcggtgcgct ggcgggtgtt tctcgtgtaa ccatctggag cgacgtcgcc ggggtataca
    13561 gtgccgaccc gcgtaaagtg aaagatgcct gcctgctgcc gttgctgcgt ctggatgagg
    13621 ccagcgaact ggcgcgtctg gcggctcccg ttcttcacgc ccgtacttta cagccagttt
    13681 ctggcagcga aatcgacctg cagctgcgct gtagctacac gccggatcaa ggttccacgc
    13741 gcattgaacg cgtgctggcc tccggtactg gtgcgcgtat tgtcaccagc cacgatgatg
    13801 tctgtttgat tgagtttcag gtgcccgcca gtcaggattt caaactggcg cataaagaga
    13861 tcgaccagat cctgaaacgt gcccaggtgc gtccactggc ggttggcgtg cataacgatc
    13921 gccagttgct gcaattttgc tacacctcag aagtggccga cagtgcgctg aaaatcctcg
    13981 acgaagcggg attacctgga gaactgcgcc tgcgtcaggg gctggcgctg gtggcgatgg
    14041 tcggggcggg tgtcacccgt aacccgctgc attgccaccg cttctggcag caactgaaag
    14101 gccagccggt cgagtttacg tggcagtccg atgacggcat cagtctggtg gcagtactgc
    14161 gcaccggccc gaccgaaagc ctgattcagg ggctgcatca gtccgtcttc cgcgcagaaa
    14221 aacgcatcgg cctggtattg ttcggcaaag gcaatatcgg ctctcgctgg ctggaactgt
    14281 tcgcccgcga gcagagcacg ctttcggcgc gtactggctt tgaatttgtg ctggcaggcg
    14341 tggtggacag ccgccgcagt ctgttgagtt atgacgggct ggacgccagc cgcgcgttag
    14401 ccttcttcaa cgatgaagcg gtcgagcagg atgaagagtc gcttttcctg tggatgcgcg
    14461 cccatccgta tgatgattta gtggtgttgg acgttaccgc cagccagcag cttgctgatc
    14521 agtatctcga tttcgccagc cacggtttcc acgtcatcag tgccaacaaa ctggcgggag
    14581 caagcgacag caataaatat cgccagatcc acgacgcctt cgaaaaaacc gggcgtcact
    14641 ggctatacaa cgccaccgtt ggtgcgggct tgccgatcaa ccataccgtg cgcgatctga
    14701 tcgacagcgg cgataccatt ttgtcgatca gtgggatctt ctccggcacg ctctcctggc
    14761 tgttcctgca attcgacggt agcgtgccgt ttaccgagct ggtggatcag gcgtggcagc
    14821 agggcttaac cgagcctgat ccgcgtgacg acctctccgg caaagacgta atgcgcaagc
    14881 tggtgattct ggcgcgtgaa gcgggttaca acattgaacc ggaccaggta cgtgtggaat
    14941 cgctggtgcc tgctcattgc gaaggtggca gcatcgacca tttctttgaa aatggcgatg
    15001 aacttaacga gcagatggtg caacggctgg aagcggcccg cgaaatgggg ctggtgctgc
    15061 gctacgtggc gcgtttcgat gccaacggca aagcgcgtgt gggcgtggag gcggtgcgtg
    15121 aagaccatcc gctggcatca ctgctgccat gcgataacgt ctttgccatc gaaagccgct
    15181 ggtaccgcga taatccgctg gtgatccgtg ggcctggcgc tgggcgcgac gtcaccgccg
    15241 gggcgattca gtcggatatc aaccggctgg cacagttgtt gtaatttcag aaatttaata
    15301 atgcccggta ctcatgtttt cgggtctatg gtttctaatg aaatatattg aattatcata
    15361 gaattaggcc ggatgaagcg tttacaacga atccggcaag aagcaataag tacatggtta
    15421 gtttatattt gcagtccggt ttgctttgca tatcggattt tctttttctt accatcctga
    15481 agttttttca tcttccctga tttttcctca ccatcattgg tcatttttcg gttgacgccc
    15541 ttcggctttt ccttcatctt tacatctgga cgtctaaatg gatagatgtg cacaacacaa
    15601 catataacta caagcgattg atgaggtaag gtatgagctt ttttcacgcc agccagcggg
    15661 atgccctgaa tcagagcctg gcagaagtcc aggggcagat taacgtttcg ttcgagtttt
    15721 tcccgccgcg taccagtgaa atggagcaga ccctgtggaa ctccatcgat cgccttagca
    15781 gcctgaaacc gaagtttgta tcggtgacct atggcgcgaa ctccggcgag cgcgaccgta
    15841 cgcacagcat tattaaaggc attaaagatc gcactggtct ggaagcggca ccgcatctta
    15901 cttgcattga tgcgacgccc gacgagctgc gcaccattgc acgcgactac tggaataacg
    15961 gtatttgtca tatcgtggca ctgcgtggcg atctgccgcc gggaagtggt aagccagaaa
    16021 tgtatgcttc tgacctggtg acgctgttaa aagaagtggc agatttcgat atatccgtgg
    16081 cggcgtatcc ggaagttcac ccggaagcaa aaagcgctca ggcggatttg cttaatctga
    16141 aacgcaaagt ggatgccgga gccaaccgcg cgattactca gttcttcttc gatgtcgaaa
    16201 gctacctgcg ttttcgtgac cgctgtgtat cggcgggcat tgatgtggaa atcatcccgg
    16261 gcattttgcc ggtatctaac tttaaacagg cgaagaaatt tgccgacatg accaacgtgc
    16321 gtattccggc gtggatggcg caaatgtttg acgggctgga tgacgatgcc gaaacccgca
    16381 aactggtggg cgcgaatatt gccatggata tggtgaagat tttaagccgt gaaggagtga
    16441 aagatttcca cttctatacg cttaaccgcg ctgaaatgag ttacgcgatt tgccatacgc
    16501 tgggtgttcg acctggttta taaatagtgt ggcttttgtg aaaatcacac agtgatcaca
    16561 aattttaaac agagcacaaa atgctgcctc gaaatgaggg cgggaaaata aggttgtcag
    16621 ccttgttttc tccctcatta cttgaaggat atgaagctaa aacccttttt tataaagcat
    16681 ttgtccgaat tcggacataa tcaaaaaagc ttaattacga tcaatttgat ctacatctct
    16741 ttaaccaaca atatgtaaga tctcaactat cgcatccgtg gattaattca attataactt
    16801 ctctctaacg ctgtgtatcg taacggtaac actgtagagg ggagcacatt gatgagcacg
    16861 tcagacgata tccataacac cacagccact ggcaaatgcc cgttccatca gggcggtcac
    16921 gaccagagcg cgggggcggg cacaaccact cgcgactggt ggccaaatca actccgtgtt
    16981 gacctgttaa accaacattc taatcgttct aacccgctgg gtgaggactt tgactaccgc
    17041 aaagaattca gcaaattaga ttactacggc ctgaaaaaag atctgaaagc cctgctgaca
    17101 gaatctcagc cgtggtggcc agccgactgg ggcagctacg ccggtctgtt tattcgtatg
    17161 gcttggcacg gcgcaggtac ttaccgttca atcgatggac gcggcggcgc gggtcgtggt
    17221 cagcaacgtt ttgcaccgct gaactcctgg ccggataacg taagcctcga taaagcgcgt
    17281 cgcctgttgt ggccaatcaa acagaaatat ggtcagaaaa tctcctgggc cgacctgttt
    17341 atcctcgcag gtaacgtggc gctggaaaac tccggcttcc gtaccttcgg ttttggtgcc
    17401 ggtcgtgaag acgtctggga accggatctg gatgttaact ggggtgatga aaaagcctgg
    17461 ctgactcacc gtcatccgga agcgctggcg aaagcaccgc tgggtgcaac cgagatgggc
    17521 ctgatttacg ttaacccgga aggcccggat cacagcggcg aaccgctttc tgcggcagca
    17581 gctatccgcg cgaccttcgg caacatgggc atgaacgacg aagaaaccgt ggcgctgatt
    17641 gcgggtggtc atacgctggg taaaacccac ggtgccggtc cgacatcaaa cgtaggtcct
    17701 gatccagaag ctgcaccgat tgaagaacaa ggtttaggct gggcgagcac ttacggcagc
    17761 ggcgttggcg cagatgccat tacctctggt ctggaagtag tctggaccca gacgccgacc
    17821 cagtggagca actatttctt cgagaatctg ttcaagtatg agtgggtaca gacccgcagt
    17881 ccggctggag caatccagtt cgaagcggta gacgcaccgg aaattatccc ggatccgttc
    17941 gatccgtcga agaaacgtaa accgacaatg ctggtgaccg acctgacgct gcgttttgat
    18001 cctgagttcg agaagatctc tcgtcgtttc ctcaacgatc cgcaggcgtt caacgaagcc
    18061 tttgcccgtg cctggttcaa actgacgcac agggatatgg ggccgaaatc tcgctacatc
    18121 gggccggaag tgccgaaaga agatctgatc tggcaagatc cgctgccgca gccgatctac
    18181 aacccgaccg agcaggacat tatcgatctg aaattcgcga ttgcggattc tggtctgtct
    18241 gttagtgagc tggtatcggt ggcctgggca tctgcttcta ccttccgtgg tggcgacaaa
    18301 cgcggtggtg tcaacggtgc gcgtctggca ttaatgccgc agcgcgactg ggatgtgaac
    18361 gccgcagccg ttcgtgctct gcttgttctg gagaaaatcc agaaagagtc tggtaaagcc
    18421 tcgctggcgg atatcatcgt gctggctggt gtggttggtg ttgagaaagc cgcaagcgcc
    18481 gcaggtttga gcattcatgt accgtttgcg ccgggtcgcg ttgatgcgcg tcaggatcag
    18541 actgacattg agatgtttga gctgctggag ccaattgctg acggtttccg taactatcgc
    18601 gctcgtctgg acgtttccac caccgagtca ctgctgatcg acaaagcaca gcaactgacg
    18661 ctgaccgcgc cggaaatgac tgcgctggtg ggcgggatgc gtgtactggg tgccaacttc
    18721 gatggcagca aaaacggcgt cttcactgac cgcgttggcg tattgagcaa tgacttcttc
    18781 gtgaacttgc tggatatgcg ttacgagtgg aaagcgactg acgaatcgaa agagctgttc
    18841 gaaggacgtg accgcgaaac cggcgaagtg aaatacacag ccagccgtgc ggatctggta
    18901 tttggttcta actctgtcct gcgtgcagta gcggaagttt acgccagcag cgatgcccac
    18961 gagaagtttg ttaaagactt cgtagcggca tgggtgaaag tgatgaacct cgaccgtttc
    19021 gacctgctgt aatctgacct tcttcagcga ctgtctttca ggcagtcgct gaagtttctt
    19081 taccggcgta tagtgtccac aggaaaacta cacactggat ctctcatgtc tgccgcagga
    19141 aagagcaacc cactggcaat cagtggcctg gttgtgctca cacttatctg gagttatagc
    19201 tggattttca tgaagcaagt caccagttac atcggtgcct tcgactttac cgccttacgc
    19261 tgcattttcg gcgctctcgt tttattcatc gtccttttat tacgtggtcg cggaatgcgc
    19321 ccgacgccgt ttaaatacac cttagccatt gccctgttac aaacctgcgg gatggttggt
    19381 ctggcgcagt gggcgttggt cagcggaggt gcggggaagg tggcgatcct gagctatacc
    19441 atgccgttct gggtggtgat tttcgccgcg ttgtttctcg gtgaacgcct gcgacgtggg
    19501 caatatttcg cgattctgat tgccgctttc ggcttatttt tggtgttgca gccgtggcaa
    19561 ctcgatttct cttcgatgaa aagtgccatg ctggcaatcc tctccggtgt tagttggggg
    19621 gcgagcgcga ttgttgctaa acgcctatac gcccgtcatc cgcgcgtgga tttattatcg
    19681 ttaacatcct ggcagatgct gtatgcggcg ctggtgatga gtgtggtcgc tttactggtg
    19741 ccgcaacgtg aaattgactg gcagcccacc gtgttctggg cgctggccta cagtgcgatt
    19801 ctggcgacgg cgctagcgtg gagcttatgg ctgtttgtgc tgaaaaactt gcctgccagt
    19861 attgccagct taagcacact ggccgttccc gtttgtggcg tactcttttc ctggtggctg
    19921 ctcggtgaga atccgggggc cgttgaaggt agcggtattg tgctgattgt gctggcactg
    19981 gcgctggtga gccgtaagaa aaaagaagct gtcagtgtaa aaagcatctg aattttttct
    20041 tcatgtgggg cgatctctta tttaacaaaa taacgataat gccccaccat ccgccagcta
    20101 aacagcacat cttcttcctg cgcgcctgcg ccaatgttat gtatcaccag cggcgtaccg
    20161 tcgcgggcga agccatctga cgccacccca atatgcgcca gcccgttatc taatcgccag
    20221 gagacaatat cgcccgcttg atagtcaccg gcatttttac tgatggggcg tgttttgttg
    20281 tggcggctaa accaggtttc cagattaggc acccgacggt gatcgatgtt gctgtccggg
    20341 cgctttaact tccatttttg cgggtactca gcaaaattct tcgccatatc ttcgtgaacc
    20401 agtttctgca aatcgacctt ctggctgcgc aatgcgcgga tcaccacatc ggaacataca
    20461 ccgcgttctt gcggaacatc accgccagga taggtaagct gcatatacgc cggatcgtaa
    20521 aatagcgtgc tgccaatttg ctgtctggca ccgtctgcga tggcaaggtt ggtattggcc
    20581 tggatttgta ccacggttgg tggaacggcg ggagatttta aggagtggct ggtaaatgcc
    20641 gttagcaggc tgagcagcgc cagtgaagct ttcatctggt tcgtccgtga aaataagagt
    20701 cacagattcg accttccggg ctaacgattg ttctgtggag aaaatgtcca gaagcggata
    20761 aaaaactcct gaacaacgtg ggagtttcag attactggca cagagtgccg gatgcggagt
    20821 gaacgcctta tccggcctac aaaagcacgc aaattcaata tattgcagag attgcgtagg
    20881 cctgataagc gtagcgcatc aggcaatttt gcgttcaaac tcccggacaa gccgggagtt
    20941 tggagtaggt tattcccact cttgcaggaa acgctgaccg tactggtcag ctaccagcag
    21001 tgcggcgtaa acctgatctg gcgtcgcgcc gccaggcatg ttgtggatgg tttcaccttc
    21061 tgcacaagat gcttttgcaa caatgcgcat tttcgccggg acatcttctt taatatccag
    21121 ttgagcgaga gttattggca aacctaccgc atggctaagt gcagctacgg tttcgatttc
    21181 ctccaccggc gcgttttcca gtaccagctg cgtcagcgta ccaaatgcca ctttttcacc
    21241 gtgataatag tgatgcgcgt ccgggatagc ggtcaggccg ttatgcaccg cgtgagccgc
    21301 cgctttcaaa accaacgccg ctcaaatagg tgttcgcttc aatcacgcgc tccagcgccg
    21361 gggttactac atgctgttcg gcggcaagca tcgctttttc gccttcttcc agcagggtgt
    21421 tgtagcacag ttcagccagt gccagcgccg cctgggtgca cttgccgccc gccatggtgg
    21481 tcgcgccgct acgagagcaa gcacgcgctt caaaccaggt tgccagcgca tcgccgatac
    21541 ccgctgctaa cagacgtgca ggtgcgcccg cgacgatttt ggtgtcgaca atgaccatat
    21601 tcgggttatt tggcaacagc agatagcggt caaactcacc ctcatcggtg tagataacag
    21661 acaatgcgct gcacggtgca tcggtagagg cgatagtcgg tgcgatcgct accggaacac
    21721 ccatgaaatg tgccagtgct ttggcagtat cgagggtttt tccgccaccg ataccgagaa
    21781 ttgcgccaca ctgcgcagtc tccgcgatgc cacgcagacg gtcgatctca ttttgcgaac
    21841 attcaccgcc aaacggcgta atttctacta ccagtccagc atctttaaag cttttctcga
    21901 cagtggattg agcaaaacct aaaacaaatt tgtcacccac cactaaccag cgttctgcca
    21961 gcggcttcag gtattcgccc agacgattaa tcacatcagc gccctggatg tatttacccg
    22021 gtgattgaat aatgcggtcc ataattgctc ctttagagat gagtagtgcc aaatgcggca
    22081 ttccagtcgt gttcgaactt ctctatagct gactctactg caggggtgtt gagcatttgt
    22141 tgcgctacat ctaagggcag ggtgatggat tcacatcctg ccagtaaaca gtccagcgcc
    22201 tgacacggcg ttttaaagct ggctgccagc accatgcttt ctggcgcgtg catttctaac
    22261 agcgtttgca gctcctgaac cgtacgaatg ccgtccccgc cctgggcatc tacgcggtta
    22321 acatacggag caacgtattt tgcccctgcc agtgcggcta ataacccttc tgcggcgcta
    22381 tatacagcgg tgccaagtgt agtaataccc tcttttttca gtattttaat tgctgccaga
    22441 ccttcggaag tcaccgggat tttcaccaca atacccggaa tagcgtcgcg caggcgcttc
    22501 gcttcttcca ccatcccctg cgcgtcgcgg ctcatggtct gagcaaacag aatgccctca
    22561 tcaccaatcg ctttttgcag acgcggcagc acttcccaga tggactcctt gctggcagcg
    22621 ataatgctcg ggttagttgt caccccggca atcgggaata tgcgtgccag acgttcgact
    22681 tctgcgacgt tagcggtgtc cagatacagt tccatgatgt ttccttttac agttccagtt
    22741 catgttgcag caggctggcg atagcgtcgg cagaggcggc gttaaccagc gcgttacgga
    22801 attcttcgtg catgatgcgg cgagcgaggc gcgagaaaat gcgcatgtgc tgatcgcccg
    22861 cagcgtgttt gttcagggtt aacataatga tgaattgcgc ttcatcatcg ccccagcgca
    22921 ccggcgcttg cagacgcgcc acgctgatgg tggattgctc aatgtgttct gatttgctgt
    22981 gtggaatggc aaaactaaag cccagaccgg tagagaaaac ggcctcgcgc gcccataagt
    23041 cggcttccag tttacgcgga tagcggcagc ggcccgccag cagcaggtta tcggtcatgc
    23101 ctttgagcac ttcttctttg ctgcgccagt cactttccag cgtgatgcac tcggcggtga
    23161 ccagcggtgc gtcctgttgg gtcatgcgga attgcgccag caggtgttcc acttccagcg
    23221 aagtacggca ggccattgcc tggttgagca acttgcggca ctcacggcta tcaagttgcg
    23281 ccatccgcgc tttcgccgcc ggaattgatg gtgcgctcat gctgagttca tccagcccta
    23341 agccgaccag caacggcagc acggaacctt tcgctcccag ctcaccgcac agaccaatcc
    23401 atttaccctg gcgatgcacc gcctgcacgg cgtaatcgag cgcccgcaag aatgccggat
    23461 tcaggctgtt gtagtgacga gtaaccttag cgttatcgcg atccactgcc agcagatatt
    23521 gcgtcaggtc attactacca atactaaaga aatcaatctc ttcgcagcat tgatcgatga
    23581 tgaacatcac cgacggcact tccagcataa tgccgagctg gatcttctca tcaaacggaa
    23641 tgtgttcgtt acgtagttgc tgtttggctt ctgccagttt ttctttcacc cataagatct
    23701 cttccattga ggagatcatc gggatcatga ttttcaggct gccgtgagcg gaggcgcgca
    23761 ggatcgagcg tagttgtgtg gtaaataacg acgcgtattc ttcataaata cgcacggcgc
    23821 gataaccgag gaacgggttt gcctctgcgg gaatgtttag ataatcaacg ggtttgtcgc
    23881 cgccaatgtc catagtgcgc acaataatgc tgcgtccgtt ggcagactcc agcgcctgac
    23941 aaaaaatgtt gtacagctcg ctttcgcccg gtgcactggt gcgatccata tagagcattt
    24001 cagtgcggaa caaaccaacg ccttccgcac cattgccgaa tgcggcctgc gcttccacgg
    24061 agtgagcgat gttagcggca atttcaatgc ggataccgtc agcggtacgg gcttgttggg
    24121 tcagccagac acgctgttgc tcacgcaggg cgtcctgtac gcgggcttct tgctgataat
    24181 aacgagctac ggcttctcct ggctcaacca caatcgcccc ggcgttgccg tcgatataaa
    24241 tcgtttgatg ctgccacgga gtgagggcat caatatccac accaaccagc gtcggaatgt
    24301 tgaacgaacg ggcaaggatc accgtatgtg aggtggtgcc gccgcttttc agtaacaatc
    24361 ctttgaggtg atttttatcc agttcgagga actggctggg ggtcagttca tcagccatac
    24421 aaatggcgga ctgcgtcagt ttgcccggtg ccgggaagcg ttgctcaccg tagatttgct
    24481 ggagtaactg gaagcagacg tcgcgtacgt ccagggcacg ttcttgcagg tatctgctgc
    24541 tggaacgggc gaactcttcg caaaagtgat tcgcactggc gacaatcgct tcggcgcagc
    24601 ttaatccggc gctgactcct gccagtaaat gttcgtgcag ggaagtatcg ccagccaggg
    24661 atcggtgcgc ttccagaatc gcgctggtag caccgtcgct atccagcaga cgaaactcaa
    24721 tgtttttcag taccagcgtc aggccgtttt ccagtgcgga ttgctcggcg tcaacgtctt
    24781 tggctgcggg aagattactc agcgcattga gatctaaaga agagatcggc gtcagaatgc
    24841 cgcctgcact accgctgcac acggtgcgga cgcggataat ttgcggattc agattggtca
    24901 gtgaaaccgg cagtggttcc agttcgtcag atttaacttc cgccagcggc gcatcgcagt
    24961 gggggaattc atcgcgcagc cattggctta aacgctggtg cccttcctgt tcgtcggccc
    25021 cggaaatcag tagctggcag ttatcaccca ccagcgtatc ggtgccaatc agcgccagag
    25081 cgcttttggc gttgccttta cggtcagtac gcaggttatg ccactcaatt tgagatgaaa
    25141 aagtattaca cagcgtttca acgtggcttg ccggacgcgc atgtacgccg ttaggtagct
    25201 cacaaataaa ttccacaatc agggccattg cctttctccc ggtacggttt tctgtttaca
    25261 ggataagagc gcacggtaac ggcctgccat gtgacaaatc tgccaaaagc tggacaaatg
    25321 taatgtaacc gtcaatttgc gacgcgtctc acaagacgcc gttttgcggc atgcttccgg
    25381 attatcgcaa gttatgaggc ggatcgcatt tttgtactga tattacaaaa gtccagtaaa
    25441 tggccttttt atccactgtt tgctccgcct gcgattgcct attgtttcct ccatcaaaaa
    25501 tatggacatg ggcttttgca agcccgggag cagcttatga atgagttggt gcagatcctg
    25561 aaaaataccc gtcagcattt aatgacgggc gtttcacaca tgattccctt cgtggtatcg
    25621 ggcggtattt tgctggcggt ttccgtcatg ttgtatggca aaggcgcagt gccggatgcc
    25681 gtagccgatc cgaatttgaa aaaactgttt gatattggcg ttgcaggttt gacgctgatg
    25741 gtgcctttcc tcgccgctta cattggttac tccattgcag agcgttctgc gctggctccg
    25801 tgcgctatcg gtgcctgggt tggtaacagc tttggtgcgg gcttctttgg tgcgctgatc
    25861 gccgggatta tcggcggcat cgtggtgcat tacctgaaga aaattccggt gcataaagtt
    25921 ctgcgctcgg tgatgcccat cttcattatt cctatcatcg gcacactgat taccgcaggc
    25981 atcatgatgt ggggcttggg cgagcctgta ggggcgttga ccaacagcct gactcagtgg
    26041 cttcagggga tgcagcaggg cagcattgtt atgctggcgg tgatcatggg gctgatgctg
    26101 gcgttcgata tgggcggtcc ggttaacaaa gtggcctatg ccttcatgct gatttgcgtt
    26161 gctcagggtg tttataccgt ggtggccatt gctgccgtgg gtatttgtat cccgccgctg
    26221 gggatggggc tggcgacgct gattggtcgt aaaaatttct ccgcagaaga gcgcgaaacc
    26281 ggcaaagcgg cactggtgat gggctgtgtt ggcgttactg aaggggcgat tcctttcgcc
    26341 gctgccgatc cgctgcgtgt cattccttcc attatggtcg gttcagtttg tggtgcagta
    26401 actgcggcgc tggtcggtgc gcagtgctat gcaggctggg gtggtctgat tgtgctgccg
    26461 gtggttgaag gtaagttggg ttatatcgca gcagtggctg tcggagcagt ggtgacggct
    26521 gtttgtgtga acgtgctgaa aagtctggcg cgtaaaaatg ggtcttcgac tgatgaaaaa
    26581 gaagacgacc tggatttgga ttttgaaatt aattaattga ggaacgagcc atgacgaaaa
    26641 ttattgcagt aaccgcatgt ccttcaggtg ttgcccatac ctatatggta gcagaggcgc
    26701 tggaaagtgc cgcgaaagcg aaaggctggg aagtgaaagt agaaacccag gggtcaattg
    26761 gtctggaaaa tgaactgact gcggaagacg tggcgagcgc tgatatggtg attctgacca
    26821 aagatatcgg catcaagttt gaagagcgtt ttgctggcaa gaccatcgtg cgcgtcaaca
    26881 tcagcgatgc agtaaaacgt gctgacgcca ttatgagcaa aattgaagct cacctggcgc
    26941 aaaccgctta agttcttctc ccgctcgcaa gggcgggttc gctttctcac aggagttcct
    27001 catgacgaat cgtatctctc gcctcaaaac tgcactgttt gccaataccc gtgaaatctc
    27061 gctggagcgg gcgctgcttt ataccgccag ccatcggcaa accgaaggcg aaccggtgat
    27121 attgcgccgg gcgaaagcaa cagcgtatat ccttgaacat gttgaaattt cgattcgtga
    27181 tgaagaactg attgccggta accgcaccgt aaaaccgcgc gccgggatta tgtcgccgga
    27241 aatggaccct tactggctgc tgaaagagct ggatcaattc ccgacgcgtc cgcaggaccg
    27301 ctttgctatc agcgaagaag ataaacgtat ctaccgtgaa gagttgttcc cgtactggga
    27361 aaaacgttcg atgaaagatt tcatcaacgg gcagatgaca gatgaagtaa aagccgcgac
    27421 caacacgcag attttcagca tcaaccagac ggataaaggc caggggcaca ttattattga
    27481 ttacccacgc ctgttgaatc acggtctggg tgagctggtg gcacagatgc agcaacattg
    27541 tcagcaacag ccggagaatc acttttatca ggccgcgttg ttactgctgg aagcctcgca
    27601 gaaacacatt ttgcgttacg ccgaactggc ggaaacgatg gcggcaaact gcacagatgc
    27661 ccagcgtcgc gaagagctgc tgactattgc ggagatctcc cgccataacg cgcaacataa
    27721 gccgcagacg ttctggcagg cgtgccagtt attctggtac atgaacatca ttctgcaata
    27781 cgaatccaac gccagttcgc tgtctttagg gcgcttcgac cagtatatgt tgccgttcta
    27841 tcaggcatct ttaacccagg gcgaagatcc ggcgttcctg aaagaactgc tcgaatcttt
    27901 atgggtgaaa tgcaacgaca tcgtgctgtt gcgctccacc agtagcgcgc gttatttcgc
    27961 cggtttcccg accggctata ccgcactgct cggcgggtta accgagaacg gacgtagcgc
    28021 ggtgaacgtg ctttcgttcc tttgccttga cgcctatcaa agcgtgcaat taccgcaacc
    28081 gaacctcggc gtgcgcacta acgccttgat cgacacgccg ttcctgatga aaaccgccga
    28141 aaccattcgc ctcggcaccg gtattccgca aatctttaac gatgaagtgg tggtgccagc
    28201 gttcctcaac cgtggcgttt cgctggaaga tgcgcgcgac tattccgtag tgggctgtgt
    28261 ggaattatct attcccggca gaacctacgg cttgcatgac atcgcgatgt ttaatctgct
    28321 gaaagtgatg gaaatctgcc tgcatgaaaa tgaaggcaat gctgcgctga cttatgaagg
    28381 tttactggag cagatccgcg ccaagatcag ccactacatc accctgatgg ttgagggcag
    28441 caatatttgt gatatcggcc atcgcgactg ggcacctgta ccgctgctct cgtcttttat
    28501 cagcgattgt ctggaaaaag gtcgcgatat taccgatggc ggcgcgcgtt ataacttctc
    28561 cggcgtacag ggaattggta tcgccaacct gagcgattct ctccatgcgt tgaaagggat
    28621 ggtttttgag caacagcgtt taagttttga cgaattgctg tcggtattaa aagccaactt
    28681 tgcaacgcca gaaggcgaaa aagtccgcgc tcgcttaatt aaccgctttg agaaatacgg
    28741 taacgatatc gacgaggtgg ataacattag cgccgaactg ttgcgccact actgcaaaga
    28801 agtggaaaaa taccagaacc cgcgcggcgg ttacttcacg ccgggatcgt ataccgtttc
    28861 tgctcacgtt ccgttgggat cggtggttgg cgcgacgcca gacggtcgtt ttgccgggga
    28921 acaactggca gacggcggct tgtcacctat gctgggtcag gacgcacaag ggccaacggc
    28981 ggtactgaag tcagtcagta agctcgataa tacgctgctg tctaacggta cgttgctgaa
    29041 cgtgaaattc actccggcga ccctggaagg tgaagcggga ttacgcaaac tggccgactt
    29101 cttacgggtg tttacccagc ttaagttgca gcatattcag tttaacgtgg tgaacgccga
    29161 cacgttgcgg gaagcgcaac agcgcccaca agattatgcc gggctggtgg tgcgcgttgc
    29221 tggatacagc gccttctttg tcgaactgtc gaaggagatc caggatgaca tcatccgccg
    29281 gacagcgcat cagctgtaac gttgtggaaa cgcgccgcga tgatgtggcg cgcattttca
    29341 acattcagcg ttattcactg aatgacggcg agggcattcg tacggtggtc ttttttaaag
    29401 gctgtccgca tctttgcccg tggtgtgcta atccggagtc gatctccggc aaaatccaga
    29461 cggtacgcag agaggcgaaa tgtctgcact gtgcgaaatg tttgcgtgat gcggatgaat
    29521 gcccctccgg ggcgtttgaa cggattggtc gcgatatcag ccttgacgct ctggaacggg
    29581 aagtgatgaa agatgacatt ttctttcgca cgtccggcgg cggcgtcacg ctttctggcg
    29641 gcgaagtgtt aatgcaggcg gagtttgcta cccgtttttt acagcgactg cggctgtggg
    29701 gtgtctcatg cgccattgaa actgccggag acgcgccagc aagcaagctg ttaccgctgg
    29761 cgaaattgtg cgatgaagtg ctgttcgatt taaaaattat ggacgcgact caggcgcggg
    29821 atgtggtgaa gatgaacctg ccacgcgtgc tggagaatct gcgtttgctg gtgagtgagg
    29881 gtgtcaacgt gatcccgcgt ttaccgttga tccctggttt cacgctcagc cgggagaata
    29941 tgcagcaggc gctggatgta ctgatcccgc tgaaaatcag gcagatccat ctgttaccgt
    30001 ttcatcagta cggcgaaccg aaataccgcc tgctggggaa aacatggtcg atgaaagagg
    30061 tgcctgcgcc gtcgtcagcc gatgtgacaa cgatgcgcga aatggcagaa cgggccggat
    30121 ttcaggttac cgtgggaggt taaaatggca tacctggtgg cagtaaccgc ctgcgtaagc
    30181 ggcgtggcgc atacttatat ggcggcggaa cggctggaaa agttgtgcca gttagagaag
    30241 tggggcgtca gcattgaaac tcagggcgcg ctgggaacgg agaatcgttt agcggacgag
    30301 gatatccgtc gggcggatgt tgctctgttg attacggata tcgagcttgc cggtgccgag
    30361 cgatttgaac attgccgcta tgtgcaatgc agcatctacg cattcctgcg tgagccgcag
    30421 cgggtaatga gcgcggtgcg caaagtgctt tctgcgccgc agcaaaccca tcttattctg
    30481 gagtagtcgg tttttctgtc agttggctgt ggtactgccg gcgatattcc gacggcgagt
    30541 gttcggtgtt tttacgaaac agacggcaga agtagttgct gtcgacaaag ccgcaggcgt
    30601 gcgccacttc ttttaccttc aggtcatagc ctttcaataa cgtcttagcg tgctccagtc
    30661 gcgtgtgatt caggtattcg ttaaaaccaa tggcccccgt tttttgaaac aggtgcgaga
    30721 gatagtttgg tgaaatatag aatgcctgtg cgacagattc gcgggtaagc gcggaggcat
    30781 agcgttcgtc gatataatcg cgaatagctt caaatagtgc ctggctgcgt gaggcggtct
    30841 ggatttggct gccgcgcaaa tcgcggcagt ggctaagcag gctggctacg ataagccttg
    30901 ccgtttgctg ctcctgcggc tgcatttgca tttcattgag cgtttgtagc agaaaagaac
    30961 ctatgcgtgg gccgcgtcgg gcgacgtgtt gcttcgccag attttgatat tgtttgccat
    31021 cccattgcac gacgctgaag ccgagttgct gtttgccaaa cagcacgcta aaggtggtag
    31081 cgggtgtttt ccattgtggg aaattccagc caccagcggc aacgtacagc acatcgccgg
    31141 gaactaacgt tgctccagcg ccggtatcaa caaactcacc ttccagcaca atttccagcc
    31201 gtggaaaatc gacctgatac gcaagatcgg gcaccgggcc gttactgctg gcaaagtaaa
    31261 tctggcgcag ggacagcggg ccgttgatca ggcgggagag caaatagctg acgtcgtgat
    31321 acatgttatt cccttaggta tgtagacggg aaaacagtaa ctggcattat taaaaaaggc
    31381 cacctgaaaa gtgtggcctg aggggagttc gatgtaaaca ttgcggcagt atcgcatccg
    31441 gcaaatagcg ccagacgcta tacaaaggtc atctactgat tacccacctg atcgccatac
    31501 ggcagagtgt cgtaatcgat cagcgcgttt ttcttgtacg ggttaccaat ccagcgggta
    31561 gtttctttga actgcggatt caccactgaa cgggttggat cgtaaccgtc gtaagataag
    31621 cccgccaaat ctgaccaggt gtggatcagt tccgccaggc tgtatttacg atcgacatcc
    31681 tgtgagaaat cacggggatg agtcgcttgc catttttccg acgtccacag caggaacgga
    31741 atggtgtaca tgtggcgcgt cgggttgtct tcgttacgcc cctgagtttt gtgcggcggc
    31801 gtgtcgtaaa cctcttcacc gtggtcagag aagtaaacca ggaatccgtt cgggtctgcc
    31861 gctttaaagt ctttaatcag gctggcaacc acatgatcgt tatacaggtt agcgttgtca
    31921 taatcgttat atgattccag ctcttctgcg cttaatcctg gaggaacatg atcggtattg
    31981 ccatcaaact tgccctgatt ttccgggtag cggtatttgt atttgatatg cgtacccagc
    32041 agatgaacga tgatcagttt cttcggcgca gggtcattca gcacatcctg gaacggcttc
    32101 agcacgttgg tgtcgtattc acgcgcactc tgcgtacgtt gctggttcat gtagtactgc
    32161 ttgtcggtct ggcgcgaaaa taccgtcagc atggtattgc gggcggtcat cgtctgctgg
    32221 ttggtgatcc agaaggtttt ataacccgcc tgtttcatca tgttcatcag cgacggctgc
    32281 gtcagataca gatccgggtt cttttcattg gcaaaggtca gcgcctgttg caggatttca
    32341 atggtgtacg gacgagacgt aactacgtta ttaaacacgg tcagattcgg atcggtttta
    32401 tgcagcgcat ccagctccgg cgtggtttca cgcggataac cgtacagact catgcgtccg
    32461 cgctgggtcg actcgccaat caccagcact aaagtgcgcg gttcgttacc cgattcatct
    32521 ttgaaattag ccagtggcgg caaggcatta ttttcattca gtaactttgt tagcgagttt
    32581 agttgctgac gatactgata atagccggtc aggaattgcc acggtgcggc aggctccata
    32641 cgcgaggcta ggttatccaa cgttttctca aacggcttgt ttttgataaa cgtattcatg
    32701 gcgatcggat gcagaatcaa gccataaagc agggcaaaag agacaacata acgccacggc
    32761 tttggaatat agaccgggcg caggcgtgtc cacagcagaa ctgccaccgc cgtataggcc
    32821 agcgcgataa gcacaatttt caggctgaaa tactggctta aatactcgct ggcttcgttg
    32881 gtgttggttt cgaacatcac aaacagaacg ctctgcgaga actcctgccc gtagatgacg
    32941 tagtagcaca gcgccgccag agaggccgtc catagcacca cgccgatcac tgcggcaata
    33001 attttaatcc gcttcggaaa gaggaatacc gggatcaacc acagcgagct gaataacagc
    33061 gagtcgcgaa tgccgttggt gccactataa ccactgatgt aaataatggc ctgtagcaga
    33121 gtagagaaaa accaaaagta gagcagtgcc caacccaggg ctttccagct agaaaggggt
    33181 ttagcctgga cttctgtgga atgcatagta agaacctgtc ttgaaaaaaa tatcgccgaa
    33241 tgtaacgaca attccttaag gatatctgaa ggtatattca gaatttgaat aaaatgcaga
    33301 cagaaatata ttgaaaacga gggtgttaga acagaagtat ttcagaaaac cctcgcgcaa
    33361 aagcacgagg gtttgcagaa gaggaagatt agccggtatt acgcatacct gccgcaatcc
    33421 cggcaatagt gaccattaac gcttgttcga cgcgaggatc cggttcctgg ccttcttttt
    33481 ctgcctggcg ggagcggtgc agcaactcgg cctgcaatac gttcagcggg tcggtgtaaa
    33541 tattccgtag ctgaatagac tctgcaatcc acggcagatc ggccatcaga tgggaatcgt
    33601 tggcaatcgc cagcaccact ttgatgtctt cttcttgcag gttgcgtaac tctttaccta
    33661 acggccacag tgctttgtct accagacgtt gatcatagta ttccgccaac cacaggtctg
    33721 ctttggcgaa gaccatctcc agcatgccga gacgcgtcga gaagaatggc caatcgcggc
    33781 acatggcttc cagctcgctc tgtttgccat cttcgaccac tttttgcagc gccgtacctg
    33841 cacccagcca ggcggggagc atcaggcggt tttgcgtcca ggcaaaaatc cacggtatgg
    33901 cgcgcagcga ctcgacgccg ccggttgggc gacgtttcgc cggacgtgaa cccaacggca
    33961 gtttgcccaa ttcttgttcc ggcgtagcgg agcggaagta aggcacaaaa tctttgtttt
    34021 cacgtacgta gccacggtag agatcgcagg agatgactga cagttcatcc ataatgcgac
    34081 gccagctctc tttcggctca ggtggtggca gcaggttggc ttccagaatc gccccggtgt
    34141 aaagcgacag gctgctgacg gtgatttctg gcagaccata tttaaagcgg atcatctcgc
    34201 cctgttcagt cacgcgcagg ccacctttca ggcttcctgg cggttgtgac agcagcgccg
    34261 catgggcagg tgcgccgccg cgaccaatgg aaccgccgcg accgtggaac aacgtcagct
    34321 caatacccgc tttttcgcag gttttgatta atgcatcctg tgcctgatat tgcgcccagg
    34381 aagctgccat cacgcccgca tcttttgctg agtcggaata gccaatcatc accatctgtt
    34441 tgccctgaat caggccgcga taccagtcga tattgagcag ctgggtcatg acatcgttgg
    34501 cgttgttcag gtcatcgagg gtttcaaaca gcggagcaac cggcatcgca aacccgatac
    34561 ccgcttcttt cagcagcagg tggacagcca gtacgtcgga cggcgttttc gccatcgaga
    34621 tcacgtaggc ggcaatggag ccttgcggcg cttcggcaat cacctggcag gtatcgagca
    34681 cttcgcgcgt ttcggcgctt ggttgccagt tgcgcggcag aagcggacgt ttggagttca
    34741 gttcgcggat caggaacgcc tgtttgtcgg cctctgacca gctttcgtag tcgccgatac
    34801 cgaggtagcg ggtcagctcg cccagcgctt cggtatggcg ggtgctctcc tgacggatat
    34861 caatacggac cagcggtacg ccgaaacatt tcacgcggcg cagggtgtcg agcagatcgc
    34921 cgttggcgat aatacccatg ccacacgcct gaagtgactg gtagcaagcg tagagcggtt
    34981 cccacagttc ttcgttttgt gtcagcagcc cttctggttt tggcagttct tcgcctttca
    35041 ggcgcgcttc cagccatgcc tgtgtcgcca tcaggcgaga acgcaggttt ttcatcagat
    35101 agcgatacgg ttctgcggca ccttcttcgc caaccagcgc cagcagttca ggggtcgctt
    35161 caaccatcga cagttcagaa accagaacct gaatatcttt caggaacaga tcggtggctt
    35221 tccagcggct gagcagcagg acgtggcggg tgatatcggc agtgacgttc gggttgccgt
    35281 cgcggtcacc gcccatccac gaggtaaaac gtaccggaac aaattcgacg ggcagtttgt
    35341 agtcgagatt ctcttccagt tgttcgttca gttcgcgcag gtaattcggt acgccttgcc
    35401 acaggctgtt ttctactacg gcaaagcccc atttggcttc atctaccggg cttggacgca
    35461 gcttacggat ttcatcggta tgccatgact gggcgatcaa ctggtgcagg cgacgcatca
    35521 gctggttgtg ttcgtagtca gcgatatctt tgttatcgag ctgttttaaa caggcgttca
    35581 cttccaccat tttgtggatc agtgtacgac gggtaatttc ggttgggtga gccgtgagga
    35641 ccagttccag cgacagcgat tccactgctt ttttgatggt gtcttcgctc agttccggct
    35701 ggtttttcag tttacgcagg gtgcgggcga tcacttccgg gttgctggca gcttcgcctt
    35761 tcggcgaaat gctgtggtat tgctcggcgg tgttggccag gttcaggaac tgactaaacg
    35821 cacgcgcaac gggcagcagc tcgtcgttcg acaaattttg taaggtggtg agcaactcct
    35881 ggcggttagc atcattgcca gcgcgtgaag atttcgacaa cttacggata gtttctacgc
    35941 gttcaagaat gtgttctccc aacgcatcct tgatggtttc tcccagcact ttgccgagca
    36001 tactgacatt actacgcaat gcggaatatt gttcgttcat attaccccag acaccccatc
    36061 ttatcgtttg atagccctgt atccttcacg tcgcattggc gcgaatatgc tcgggctttg
    36121 cttttcgtcg tcttttataa agccacgtaa aagcggtgac gtcaaatgct gcgaaatcgc
    36181 ttcagcaaac gaataaatag caggaattta cgccattaaa ttcacgacgc tttaaataag
    36241 cgtaacttat ggaaatgtta aaaaatcgcc atacataaca ccaaaggtgt aggtcggata
    36301 agatgcgcaa gcatcgcatc cgacattatt gcggcaccgg agtttggcaa cggtgccgga
    36361 tgcggcgcga gcgccttatc cggcctacgt tttaatgcca gcaaaaatgg tgaattacct
    36421 ggataatcaa ctcacgggtg ggtttaataa accgcgtctc cagatattca tcaggctgat
    36481 gagcttgatt gatggaaccc ggaccgagca ccagcgtcgg gcataacgtt tgaataaacg
    36541 gcgcttcggt acagtaattc accacttcgg tttttgctcc gagcaatttc tcaaccactt
    36601 caaccagttg atgattcggt gggcattcat agccagggat cggcggatgc agctcgtcga
    36661 ccgtcagacg acccggccag cgttcgctca ccggagccaa tgcatcgttg agcaaaccat
    36721 taagttcatt gagtgtcatg ccaggctgcg gacgaatatc catatgcaac tcacagcaag
    36781 cgcaaatacg gttagaagcg tcgccaccgt gaatatgccc gaggttgagc gtagggtatg
    36841 gcacggtaaa tgcttcgtag tgataacgtt ctttcaggtt atcgcgcaat tgcaaaatat
    36901 gcccgatggc gtcgtgcatt agttcgatag cgttaactcc gcgtgctgga tcgctggagt
    36961 gccccgactg gccctgaata cggatggcgt tagagatatg acctttatgt gcgcgtaccg
    37021 gttgtagtga cgtcggttcg ccaatgatgg cgcaatccgg gcgcagggcg gtagtttcgg
    37081 caaaataacg cgctccggcc atactggttt cttcatcagc agtcgccaga atgtagagcg
    37141 gttttttcag tttcgtgacg tcgacatcgc gtagcgcatc aaggataaac gcaaaaaagc
    37201 ctttcatgtc ggcggtgcct aagccgtaaa gcttgccgtc atgctccgtc agtgtaaacg
    37261 gatcgcgcgt ccagcgaccg tcatcaaatg gcaccgtatc ggtatgcccc gccagcaaca
    37321 agccgccagc cccctgtccg taactggcca gcatattaaa tttgttgcga gttcctggaa
    37381 caggctgcac ttccacattg aagcccaaat ctttaaacca gtccgccagc agagtgatta
    37441 aatctgcatt gctttgatcg agtgcctctt ccgtagcgct tattgaaggt gtggcaatca
    37501 gagcgcggta aatctcgata aatggcggta atttgttttt cattgttgac acacctctgg
    37561 tcatgatagt atcaatattc atgcagtatt tatgaataaa aatacactaa cgttgagcgt
    37621 aataaaaccc accagccgta aggtgaatgt tttacgttta acctggcaac cagacataag
    37681 aaggtgaata gccccgatgt tgaatacgct gattgtgggt gccagcggct acgctggcgc
    37741 agagctagtg acctatgtaa atcgccatcc gcatatgaac ataaccgctt tgactgtttc
    37801 agcgcaaagc aatgatgcgg gaaagttaat ctccgatttg catccgcagc taaaaggcat
    37861 cgttgagctg ccgttgcagc cgatgtcgga tatcagcgag tttagcccag gggtggacgt
    37921 agtgtttctc gccaccgccc atgaagttag ccacgattta gcgccgcagt ttcttgaagc
    37981 gggctgcgtg gtgttcgacc tttccggcgc gtttcgtgtt aacgacgcca ccttctatga
    38041 aaaatattac ggctttaccc atcaataccc ggaactgctg gaacaggcag cctacggtct
    38101 ggcggagtgg tgcggtaata aattaaaaga agcgaatttg attgcggtgc caggctgtta
    38161 tccgacggcg gcacagctgg cgctgaaacc gttgattgat gccgatcttc ttgacctcaa
    38221 tcagtggccg gtgatcaacg ccaccagcgg cgtgagcggt gcaggacgta aggcagcgat
    38281 ttcaaatagt ttttgtgaag ttagcctgca accgtatggc gtctttactc atcgccatca
    38341 accagagatc gccacacacc tcggtgctga cgttatcttc accccacatc tgggcaattt
    38401 cccgcgcggc attctcgaaa ccattacctg ccgcctgaaa tcgggtgtga cccaggcgca
    38461 agtcgcgcaa gcgttacagc aggcgtatgc ccataaaccg ctggtgcggc tgtatgacaa
    38521 aggcgttccg gcgctgaaaa atgtcgttgg gctgccattt tgcgatatcg ggtttgccgt
    38581 tcagggcgag catctgatta ttgtggcgac cgaagacaac ttactgaaag gcgcggcggc
    38641 acaagcggta cagtgcgcca atattcgttt cggctatgcg gaaacgcagt ctcttattta
    38701 agggtgcaat gatgaatcca ttaattatca aactgggcgg cgtactgctg gatagtgaag
    38761 aggcgctgga acgtctgttt agcgcattgg tgaattatcg tgagtcacat cagcgtccgc
    38821 tggtgattgt gcacggcggc ggttgcgtgg tggatgagct gatgaaaggg ctgaatctgc
    38881 cggtgaaaaa gaaaaacggc ctgcgggtga cgcctgctga tcagatagac attatcaccg
    38941 gagcactggc gggaacggca aataaaaccc tgttggcatg ggcgaagaaa catcagattg
    39001 cggccgtagg tttgtttctc ggtgacggcg acagcgtcaa agtgacccag cttgatgaag
    39061 agttaggtca tgttggactg gcgcagccag gttcgcctaa gcttatcaac tccttgctgg
    39121 agaacggtta tctgccggtg gtcagctcca ttggcgtaac agacgaaggg caactgatga
    39181 acgtcaatgc cgaccaggcg gcaacggcgc tggcggcaac gctgggcgcg gatctgattt
    39241 tgctctccga cgtcagcggc attctcgacg gcaaagggca acgcattgcc gaaatgaccg
    39301 ccgcgaaagc agaacaactg attgagcagg gcattattac tgacggcatg atagtgaaag
    39361 tgaacgcggc gctggatgcg gcccgcacgc tgggccgtcc ggtagatatc gcttcttggc
    39421 gttatgcgga gcagcttccg gcactgttta acggtatgcc gatgggtacg cggattttag
    39481 cttaagtttt gttagccgga ggagcagctt tccggcattg aatttcaaaa taaggaaaca
    39541 gagttatggc actttggggc gggcgtttta cccaggcagc agatcaacgg ttcaaacaat
    39601 tcaacgactc actgcgcttt gattaccgtc tggcggagca ggatattgtt ggctctgtgg
    39661 cctggtccaa agccttggta acggtcggcg tgttaaccgc agaagagcag gcgcaactgg
    39721 aagaggcgct gaacgtgttg ctggaagatg ttcgcgccag gccacaacaa atccttgaaa
    39781 gcgacgccga agatatccat agctgggtgg aaggcaaact gatcgacaaa gtgggccagt
    39841 taggcaaaaa gctgcatacc gggcgtagcc gtaatgatca ggtagcgact gacctgaaac
    39901 tgtggtgcaa agataccgtt agcgagttac tgacggctaa ccggcagctg caatcggcgc
    39961 tggtggaaac cgcacaaaac aatcaggacg cggtaatgcc aggttacact cacctgcaac
    40021 gcgcccagcc ggtgacgttc gcgcactggt gcctggccta tgttgagatg ctggcgcgtg
    40081 atgaaagccg tttgcaggat gcgcttaagc gtctggatgt cagcccatta ggctgtggcg
    40141 cgctggcggg aacggcctat gaaatcgacc gtgaacagtt agcaggctgg ctgggctttg
    40201 cttcggcgac ccgtaacagt ctggacagtg tttctgaccg tgaccatgtg ttggaactgc
    40261 tttctgctgc cgctatcggc atggtgcatc tgtcgcgttt tgctgaagat ctgattttct
    40321 ttaacaccgg cgaagcgggg tttgtggagc tttcggaccg cgtgacttcc ggttcatcat
    40381 taatgccgca gaagaaaaac ccggatgcgc tggagctgat tcgcggtaaa tgcggccggg
    40441 tgcagggtgc gttaaccggc atgatgatga cgctgaaagg tttgccgctg gcttacaaca
    40501 aagatatgca ggaagacaaa gaaggtctgt tcgacgcgct cgatacctgg ctggactgcc
    40561 tgcatatggc ggcgctggta ctggacggca ttcaggtgaa acgtccgcgt tgtcaggaag
    40621 cggcgcagca ggggtatgcg aacgccactg aactggcgga ttacctggtg gcgaaaggcg
    40681 tacctttccg cgaggcgcac catattgtgg gtgaagcggt ggtggaagcc attcgtcagg
    40741 gcaaaccgct ggaagatctg ccactcagtg agttgcagaa attcagtcag gtgattggcg
    40801 aagatgtcta tccgattctg tcgctacaat cgtgcctcga aaagcgtgcg gcaaaaggcg
    40861 gcgtctcacc gcagcaggtg gcgcaggcga ttgcttttgc gcaggctcgg ttagagtaat
    40921 ttgtttatat tgctcccctg aaaatgggga gtttaccacg cctacaaagt gcgacacttc
    40981 gtaggcggat aaggcgttta cgccgcatca ggcagaaatt tgtcagcatt c
//
LOCUS       DAJTYF010000021        38754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTYF010000021 DAJTYF010000000
VERSION     DAJTYF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.021"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO590_001259"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO590_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6636695.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..580
                     /gene="hisL"
                     /locus_tag="OO590_001260"
     CDS             530..580
                     /gene="hisL"
                     /locus_tag="OO590_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6636696.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    556..679
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            726..1625
                     /gene="hisG"
                     /locus_tag="OO590_001261"
     CDS             726..1625
                     /gene="hisG"
                     /locus_tag="OO590_001261"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6636697.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1631..2935
                     /gene="hisD"
                     /locus_tag="OO590_001262"
     CDS             1631..2935
                     /gene="hisD"
                     /locus_tag="OO590_001262"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6636698.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2932..4002
                     /gene="hisC"
                     /locus_tag="OO590_001263"
     CDS             2932..4002
                     /gene="hisC"
                     /locus_tag="OO590_001263"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6636699.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4002..5069
                     /gene="hisB"
                     /locus_tag="OO590_001264"
     CDS             4002..5069
                     /gene="hisB"
                     /locus_tag="OO590_001264"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6636700.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5069..5659
                     /gene="hisH"
                     /locus_tag="OO590_001265"
     CDS             5069..5659
                     /gene="hisH"
                     /locus_tag="OO590_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6636701.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5659..6396
                     /gene="hisA"
                     /locus_tag="OO590_001266"
     CDS             5659..6396
                     /gene="hisA"
                     /locus_tag="OO590_001266"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6636702.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6378..7154
                     /gene="hisF"
                     /locus_tag="OO590_001267"
     CDS             6378..7154
                     /gene="hisF"
                     /locus_tag="OO590_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6636703.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7148..7759
                     /locus_tag="OO590_001268"
     CDS             7148..7759
                     /locus_tag="OO590_001268"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6636704.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="OO590_001269"
     CDS             complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="OO590_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6636705.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="OO590_001270"
     CDS             complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="OO590_001270"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR6636706.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEDYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="OO590_001271"
     CDS             complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="OO590_001271"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6636707.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11999..12358)
                     /locus_tag="OO590_001272"
     CDS             complement(11999..12358)
                     /locus_tag="OO590_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636708.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(13008..13571)
                     /locus_tag="OO590_001273"
     CDS             complement(13008..13571)
                     /locus_tag="OO590_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636709.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13779..14717)
                     /locus_tag="OO590_001274"
                     /pseudo
     CDS             complement(13779..14717)
                     /locus_tag="OO590_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14780..15154)
                     /locus_tag="OO590_001275"
     CDS             complement(14780..15154)
                     /locus_tag="OO590_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6636710.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFELLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15158..16306)
                     /gene="wzy"
                     /locus_tag="OO590_001276"
     CDS             complement(15158..16306)
                     /gene="wzy"
                     /locus_tag="OO590_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR6636711.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16326..17231)
                     /gene="rfbG"
                     /locus_tag="OO590_001277"
     CDS             complement(16326..17231)
                     /gene="rfbG"
                     /locus_tag="OO590_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR6636712.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17224..18114)
                     /locus_tag="OO590_001278"
     CDS             complement(17224..18114)
                     /locus_tag="OO590_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR6636713.1"
                     /translation="MNSNIYAVIVTYNPELKNLNELITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18144..19400)
                     /gene="wzx"
                     /locus_tag="OO590_001279"
     CDS             complement(18144..19400)
                     /gene="wzx"
                     /locus_tag="OO590_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR6636714.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19416..19961)
                     /gene="rfbC"
                     /locus_tag="OO590_001280"
     CDS             complement(19416..19961)
                     /gene="rfbC"
                     /locus_tag="OO590_001280"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6636715.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19966..20844)
                     /gene="rfbA"
                     /locus_tag="OO590_001281"
     CDS             complement(19966..20844)
                     /gene="rfbA"
                     /locus_tag="OO590_001281"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6636716.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20903..21802)
                     /gene="rfbD"
                     /locus_tag="OO590_001282"
     CDS             complement(20903..21802)
                     /gene="rfbD"
                     /locus_tag="OO590_001282"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6636717.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21802..22887)
                     /gene="rfbB"
                     /locus_tag="OO590_001283"
     CDS             complement(21802..22887)
                     /gene="rfbB"
                     /locus_tag="OO590_001283"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6636718.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23260..24153)
                     /gene="galF"
                     /locus_tag="OO590_001284"
     CDS             complement(23260..24153)
                     /gene="galF"
                     /locus_tag="OO590_001284"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6636719.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24328..25722)
                     /gene="wcaM"
                     /locus_tag="OO590_001285"
     CDS             complement(24328..25722)
                     /gene="wcaM"
                     /locus_tag="OO590_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6636720.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25733..26953)
                     /gene="wcaL"
                     /locus_tag="OO590_001286"
     CDS             complement(25733..26953)
                     /gene="wcaL"
                     /locus_tag="OO590_001286"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6636721.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSYEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26950..28230)
                     /gene="wcaK"
                     /locus_tag="OO590_001287"
     CDS             complement(26950..28230)
                     /gene="wcaK"
                     /locus_tag="OO590_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6636722.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRSYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMIADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28410..29888)
                     /gene="wzxC"
                     /locus_tag="OO590_001288"
     CDS             complement(28410..29888)
                     /gene="wzxC"
                     /locus_tag="OO590_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6636723.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVSVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29890..31284)
                     /gene="wcaJ"
                     /locus_tag="OO590_001289"
     CDS             complement(29890..31284)
                     /gene="wcaJ"
                     /locus_tag="OO590_001289"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6636724.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDTKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31339..32709)
                     /gene="cpsG"
                     /locus_tag="OO590_001290"
                     /gene_synonym="manB"
     CDS             complement(31339..32709)
                     /gene="cpsG"
                     /locus_tag="OO590_001290"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6636725.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32814..34250)
                     /gene="cpsB"
                     /locus_tag="OO590_001291"
     CDS             complement(32814..34250)
                     /gene="cpsB"
                     /locus_tag="OO590_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6636726.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASSEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGHHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34253..35476)
                     /gene="wcaI"
                     /locus_tag="OO590_001292"
     CDS             complement(34253..35476)
                     /gene="wcaI"
                     /locus_tag="OO590_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6636727.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLLFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35473..35952)
                     /gene="gmm"
                     /locus_tag="OO590_001293"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35473..35952)
                     /gene="gmm"
                     /locus_tag="OO590_001293"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6636728.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35952..36920)
                     /gene="fcl"
                     /locus_tag="OO590_001294"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35952..36920)
                     /gene="fcl"
                     /locus_tag="OO590_001294"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6636729.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36923..38044)
                     /gene="gmd"
                     /locus_tag="OO590_001295"
     CDS             complement(36923..38044)
                     /gene="gmd"
                     /locus_tag="OO590_001295"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6636730.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38070..38618)
                     /gene="wcaF"
                     /locus_tag="OO590_001296"
     CDS             complement(38070..38618)
                     /gene="wcaF"
                     /locus_tag="OO590_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6636731.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38634..>38754)
                     /locus_tag="OO590_001297"
     CDS             complement(38634..>38754)
                     /locus_tag="OO590_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR6636732.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10349 a         9688 c         8721 g         9996 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgag ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc gatgtgtgct
      601 ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac
      661 cttccggggg cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata
      721 agaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg
      781 atgactcacg cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc
      841 tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc
      901 ccggtctggt aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag
      961 aagagctgct taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc
     1021 tggatttcgg cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc
     1081 cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc
     1141 tcgaccagaa aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc
     1201 cgcgtgccgg gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag
     1261 ctaacggcct gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg
     1321 atggcgaaat ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg
     1381 gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctag
     1441 atgaagtcat cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg
     1501 atcaacagcg cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg
     1561 aaaaactgaa agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg
     1621 agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca
     1681 acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa
     1741 cgatattctc gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa
     1801 gtttgataaa accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag
     1861 cgaacgcctg agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac
     1921 cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag gcgtgcgttg
     1981 ccagcaggtc acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc
     2041 gctcttctca acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt
     2101 ggtgctgtgc tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga
     2161 tgtgcaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac
     2221 ggaatctgtg ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc
     2281 aaaacgccag gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc
     2341 ggaagtgctg gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct
     2401 ttctcaggct gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat
     2461 ggcgcgtcgc gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac
     2521 cacccgccag gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt
     2581 ggagatctcc aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga
     2641 actggtcgat ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc
     2701 ggcaggcgac tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac
     2761 ctgttccagc ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa
     2821 agaaggtttc tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac
     2881 cgcccacaaa aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc
     2941 gtgactatta ctgatttagc gcgtgaaaac gtccgcaacc tgacgccgta tcagtcggcg
     3001 cgtcgtctgg gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg
     3061 gagtttaagc ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg
     3121 attgaaaatt acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggt
     3181 gcggacgaag gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc
     3241 ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa
     3301 tgccgcacag tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag
     3361 ctggacggcg taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc
     3421 aatccgcagg attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc
     3481 gatgaagctt atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat
     3541 ccgcacctgg cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc
     3601 ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac
     3661 ccgctctcga cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc
     3721 gccatgcgcg aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg
     3781 aaagagatcc cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc
     3841 tttaaagcct ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat
     3901 cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa
     3961 agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt
     4021 ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt
     4081 ttgacaaact cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagagg
     4141 gctacaagct ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag
     4201 cggattttga tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt
     4261 ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga
     4321 aagtaaaact ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg
     4381 taattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac
     4441 gctacgaccg cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc
     4501 gttacgccca tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg
     4561 atcgcgaagg tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc
     4621 agatcgccac ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg
     4681 acgatcacca caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc
     4741 tcggcgacaa acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg
     4801 cccgctgcgc actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct
     4861 accagcgtgt gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt
     4921 acaccatggg cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag
     4981 aaagcctgtt caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca
     5041 ccctgccctc gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc
     5101 caacctgaac tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg
     5161 tgacccggac attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca
     5221 agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca
     5281 accggtgctg ggtatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa
     5341 tggcgtcgac ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct
     5401 gccactgcca catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca
     5461 ggggattgaa gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc
     5521 gtggaccatc gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa
     5581 cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa
     5641 aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg
     5701 cgtctccatc agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc
     5761 ttacaggatt acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca
     5821 aaagatccgg ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc
     5881 ccggtgcagg ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct
     5941 ggcgttgcgc gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc
     6001 tggtttgaac gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag
     6061 caaggcaaca agcaagtggc agtcagcggc tggcaagaga actcgggcat ttcactggaa
     6121 caactggtgg aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg
     6181 cgcgacggca cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat
     6241 ccgcaagtgg cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta
     6301 cgtggcactg gcgtacgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc
     6361 gtgaaggagg ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg
     6421 gtcaggtggt gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac
     6481 tggcaaaacg ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt
     6541 ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca
     6601 ttccgttttg tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct
     6661 ttggcgcgga taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc
     6721 gcctggccga tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg
     6781 ccgaaaccgg taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca
     6841 ctcaatggga aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg
     6901 tcctcaacat gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa
     6961 aagtgcgtga agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac
     7021 acttcctgga agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc
     7081 acaaacaaat aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga
     7141 tcaggatatg ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct
     7201 gatgccggtg attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa
     7261 cccggaagcc ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa
     7321 acagcgactg tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc
     7381 cccggactgc gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca
     7441 caaaggcacc agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact
     7501 ggaacaactg ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact
     7561 gtatgccagc ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc
     7621 actggcagca acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta
     7681 tcacctgctg gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct
     7741 gcataaacgg catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga
     7801 gccattgccc gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg
     7861 ataagatgcg ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc
     7921 gtttctctta aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc
     7981 caacacaatc cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc
     8041 ctttttcgga ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg
     8101 aatatcaaga tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata
     8161 gtagtttggt gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc
     8221 ttcgctccct agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg
     8281 ctttgtcacc tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag
     8341 atctttctgc tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg
     8401 tcccagagca atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac
     8461 ttgctgaatg tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga
     8521 aacagtcaat ggtaattgtt ggttcttaac agaaggttcg atggtaagtt tttctggctc
     8581 ttcctgatta tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa
     8641 cgtctcctgc aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt
     8701 gttatagcca gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt
     8761 ctccttcgct actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat
     8821 aattgtcatc ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg
     8881 gtcatggttt tgcccagaaa cattattatt ttctactctc atcattaccc taactgacga
     8941 aaatttccaa gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata
     9001 aaaataatga attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa
     9061 caagatgtta gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa
     9121 gctcttctgc catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat
     9181 cacgttccag gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca
     9241 cttcaacgcc tttcgccttg atacgcttca taatcccctg aatagaagac gtacggaagt
     9301 tatctgaccc gctcttcata atcagacgat aaatacccac cacttgcggt ttgcgtgaca
     9361 aaatggcatc ggcaataaaa tctttacgcg tgcggtttgc atcgacaatt gccgagatca
     9421 ggttattcgg cacagactgg tagttcgcca gtaactgctt ggtatctttc ggcagacaat
     9481 aaccaccata accaaacgac ggattgttgt aatggttgcc aatacgcgga tcgagacaaa
     9541 cgccttcgat aatctggcga gtattcagac ctaaactttc tgcataacta tccagttcat
     9601 taaagtatgc tacgcgcatc gccagatagg tattagcgaa aagtttaatc gcttctgctt
     9661 cagtggagtc ggtaaacagg gtcgggatat tttgcttaat cgcgccttcc tgtaacaacg
     9721 cagcaaaacg ttctgcgcgt tctgaacgct caccgatgac aatacgtgac ggatggagat
     9781 tatcgtaaag ggctttacct tcgcggagga attccggaga gaaaataata ttttcagtac
     9841 gatatttctt atgcatcgct gcggtaaaac caacgggaac cgttgatttg atgaccataa
     9901 ccgcataagg atttatctca actacgtctt taattactga ttctacactg gatgtattga
     9961 aataattagt tttaggatca tagtcggttg gagtggcgat gatgacataa tcagcatccc
    10021 ggtagtcttc atttttatct aatgtggcat taaagtgtat tttatctgat tgcaaaaact
    10081 gctgaatttc cttatcaaca ataggagata tccgatcatt cagcatagca acgcgtgacg
    10141 gtaaaatatc taatgccaca acctcatgat tttgtgcgat tagaagcccg tttgacaagc
    10201 ctacatagcc agtaccggaa atggtgattt tcatttcatg ctctcagaat taacttaact
    10261 gtgaatcatg atgtttttag catcgtgata agatgctaaa agttttaacg ctacgttgtc
    10321 agatggtcaa cgcaacgaaa aacaattcag attttcttta taagaatagc tgatgtttat
    10381 taagttaata ttaagcagtg aaatttagtc tataagatat ttggcaaaaa aaagcccggt
    10441 gcaatatacg ccgggcctca attttattgt tggttaaatc agattaatcc agccattcag
    10501 tatggaacac accttcttta tcaatgcgct tataagtatg cgccccgaaa tagtcacgct
    10561 gtgcctggat caggttcgca ggcagaaccg cggcacggta gctatcgtaa taggcaaccg
    10621 cagcggcgaa ggtcggaacc gggataccgt tctgtactgc ataagcgacg acatcgcgca
    10681 gcgcctgctg gtagtcatcg gcaatttgct tgaagtacgg tgccagcagc aggttagcga
    10741 tctgcggatt ttcggcataa gcatcggtga ttttctgcag gaactgcgca cggatgatgc
    10801 agccagcacg gaaaatcttc gcgatttcgc cgtagttcag atcccagttg tattcttcag
    10861 acgcagcacg cagctgagag aagccctgag cgtaagaaac gattttgccc agatacagcg
    10921 cactgcgaac tttttcgatg aactcagcct tgtcgcctgc tgactgagct tgcggaccag
    10981 agagaacttt agatgcggca acgcgctgat ctttcagaga agagatataa cgtgcaaaca
    11041 cagactcggt aatcagcgac agcggttcgc cgagatccag cgcgctctgg ctggtccatt
    11101 taccggtgcc tttgtttgct gcttcatcca gaatcacatc aaccaggtag ttaccgtctt
    11161 catctttttt ggtgaagata tctttagtaa tgtcgatcag gtagctgcta agttcaccgt
    11221 tattccactc ggtgaaagtc tgggccagtt cttcattaga tagattcagg ccacctttga
    11281 gcagagaata cgcttcagca atgagctgca tatctccgta ttcaatacca ttgtgaacca
    11341 tcttcacata atggcctgcg ccgtcggcac caatataagt aacgcatggc tcgccgtctt
    11401 ctgccacggc agcgatttta gtcaggatag gcgcaaccag ctcataagcc tctttctgcc
    11461 cgccaggcat aatagaagga cctttcagcg cgccctcttc accgccggaa acaccggtac
    11521 cgatgaagtt aaagccctct gctgaaagct cacgattacg acgaatggtg tcctggaaga
    11581 aggtattacc accatcaatg atgatgtcac ctttatcgag gtatggtttc agggaatcaa
    11641 tcgcagcatc cgtgcctgca cctgctttca ccattaacag gatgcgacga ggcgtttcca
    11701 gagattcaac aaactctttc accgtatagt aaggagccag tttcttgcct ggattttcag
    11761 caatcacttc ttccgtcttt tcgcgggagc ggttgaaaat agagacggta taaccacgac
    11821 tttcgatgtt gagcgcaagg ttgcgcccca tcactgccat accgactacg ccgatctgtt
    11881 gctttgacat tgtttactcc tgtcttctat attattaacc gtctattaac cgttatatta
    11941 ttaaccgtcg tgattttcat cttctaatca aggaatacat atggttacag catgcttctt
    12001 actttatagc gataagtatg ccattgggtg tatccataag aactttgaat tttggatact
    12061 tttctggatt aaaattagca tagcttcgtt tagatataac aataaacgaa ctattaaaag
    12121 atttcatctt ctctattaaa tcatcctgtg ggattacctc acactcggga ttactaatag
    12181 tccaatcact atatcttgct aatccaaaga cgtcaccaat tgccatccct ttatagaagt
    12241 aaataccatc ctccaagcct aattggataa gattatctcc atataactca ctatattcac
    12301 ttgctttctt catcagttca tagctatagc gactttctaa gcgtgaatta aaattcaagt
    12361 attcagaata aaaatatttg aattcaaaat gcaacgatcg ccccgaaaaa aaaataatac
    12421 acacaaggat aaaatatcgt cccaacttgt aatttgtaat gttgatatct tttattaaac
    12481 ttgcaattga gatcgcaacc agcaatactg acaatgggta aatgggtgat aaatacctgt
    12541 caccttgcgt aacagaaaac caaaataaga catatgttaa aacaatcgag aaaaataccc
    12601 aaagtatttt attattccta agtgtaaaga cagacagtat agcataaaaa acaacatcac
    12661 tgccaacttt attaattgct tgttgcaaat ctaatgtggc aggacttata ccatgtcgag
    12721 cttgttctgc tgtttgaact tgtagatcaa tttcattcca taggtagtga ccaaatatat
    12781 ttcctccagc aggactaaaa ggatcaccag agatccaaaa actacggaca taccaataaa
    12841 tgccaccaag aattgttgcc aagctaaaaa taagaaatgt ctgtatgttt ctctttttcg
    12901 ctatcaatat aacaaatatg atggctccat atatggcacc aaatagtttt acgccacaag
    12961 caattccaat aaaaaaacca cataaaaaaa ttaattcatt ttgtgtttta ttgttttctg
    13021 agcgaatata tataagaata ctggctgcag caaaacaaag atagcgttaa atcaatatat
    13081 gcataaccaa gatgatcttt aagtggattg atagtaaata gtaggattgt agcaataatg
    13141 ctaggataat aagttttcgt aatttcccat gaacagccaa caagtccaca ggctattaca
    13201 aaaaaacaga tatttgctaa aaactgagct aaccttacat ctcctaactg taaacctaaa
    13261 gccattaaaa gatctgcatt ttgcgggaac agaggaaaac gcaaatattg ttcaacaaca
    13321 attgcatggt gctcaactaa tgaacgagcc aaaggtaagt gatacatagt atcatcccaa
    13381 tcaccgggag ctcgcaggga cgataacaat gtaaccacaa aaatcaaaac aagaaataaa
    13441 ataacaaaca gcgtactgaa ctcaatatta tttattgcca gccatctaaa ttgctgacgt
    13501 ttaataatgc ttaaaaccca acacaccgta aaaattaaaa agatagcaat cttattaaat
    13561 tgcccgacca tgcccagaaa gtaaattatc aaaatattta aagacatccc tatgcatagg
    13621 caataaaaaa ataaagaaaa tttgttaagt cctgccaccc cctccttaaa aaaagatgac
    13681 aatacaatat taccaatgac aaaaaatgtc attccaaaag caaaacaata cacaacatac
    13741 aaatacaacg gcaattcaat catagtaaaa tactctattt acattatcag tttatagttt
    13801 ttttcagaaa gataaaataa tgccttcatt tcacgtctag ttgttgtatt tgaatgcaaa
    13861 ataatgccac atacaaatgc cattattgac gataacatga tggaggcagt taaaaaggca
    13921 gtcggaaatc ttctcaccaa acctgtatca atataatcaa caatgatggg caatcctaaa
    13981 attatagaca aagcagcaag taaaacgccg ataatactaa aaaagtatag tggccgttca
    14041 agcgagtaaa gtttaattat tgttttcaga atcttgaacc catcactcca agttgatagt
    14101 ttgctcacac tcccttcaga tcgctcgcca tattttgtat taacctcacc atacttcata
    14161 cgtaattcta aagcatgaat tgtaagctca gtttcgattt caaatcccct tgatagacat
    14221 ggaaaagatt taacatagcg tctggaaaat acacgatacc ccgacaacat atcagtaaaa
    14281 ttccctttaa agatagtctg aacagtacca gtcaacagtt tatttcctca gcgatgccct
    14341 tttctgtatg tattttgatc tccattctct tgacgacatc ctacaaccat atccaattga
    14401 ttttttatga gatggttaat catttctgga gcagaacttg catcataagt gccatctcca
    14461 tcaaccatta gataaatatc cgcatctaca tctgagaaca tacgtcttac gacattcccc
    14521 ttgcctttca gaggaacaga gtgaactgtt gctccagcat cctctgccac ttgtgctgta
    14581 ctgtcagtcg aattattatc aaatacatat atagaagcag ttggaatatt cgttttgaaa
    14641 tcatttatga cttttgctat tgccttctgc tcattataac aaggaataat tacagcgata
    14701 ttaaaagagt gatacataag cccctcatat aaactattta gctaatgtgc aagtaattgt
    14761 gtttttaaca aatcgtttac taacgaaaaa tgaaatattt tgatgcgcaa tatcctaaaa
    14821 ccaaactaaa aagagaaaag gtgaacaatg taaatattgg agataaagct aagagttcaa
    14881 ataaaacgcc gaataaataa ctcatcgccc ccataaaaaa tataaatata aaatatctag
    14941 ccttggttgc agatacttta aatgagcatc ttgcatttgc aaaaaaagaa aaggttgctg
    15001 cacatataaa accaacaatg ttacttcggc cttgaccaaa agctaaatta ttataacacg
    15061 cataaaatac cccccaatgt aaagcagtat tgacaattcc aattaaaaaa taactaaaaa
    15121 aagaactggt caataacttc cctattttta acatccttta ttttgctcca gaagtgaggt
    15181 tattactaat ttggatattt tctatagaaa atacccataa aactacaacg cctataataa
    15241 aaattcccat gggtgtaaaa agaaatggat tagttataga aacggtaata ctgaaaaaaa
    15301 gtattaacga catcattaat gatgtttttg tactatttaa aaaatagcct ttcaaaaaga
    15361 taagcaacat aggaactaat gatataaata caccaataac gcctgttttc tgaagtatct
    15421 caagaggtac tatttcaatc cttaatcgat ctaatataaa tgaaccaaat cctcttccaa
    15481 acaagaacgt cgctaaatca acatttttat aataaaaata taaatcatta aatctaacag
    15541 agtcagaatc ttcactgcgt gatccgacca tgtaaagcat aattataaat agaacgctgc
    15601 ccaatattat tataatagta tttaaatata gcttatgtaa tttaaaactg gctaataaca
    15661 gtgataatgt tgtaaaaagt aatacacctc ttgattctgt caataatatc gcaagcacta
    15721 ttatgagaca ttttaaatat tttttgtcga caaaactgat aatcgcaccg acaccaaaaa
    15781 agtagaagga tttggaaaaa aaagccccat cgggacgaaa aaagaactcg ctagtcaatg
    15841 ataagtgttc ataaattaaa ttaaaattta acaaaccgaa attcaacaaa attatgtatg
    15901 aaatatataa cagtgatcca tataatgcaa atgcattaac tattttaata aaaatctcac
    15961 ataaaaatac ttttaaagta tatcttagtg taaatagata tataagtgac agcataaaaa
    16021 ggtatggttg cacatcaaac agtatatcat ttattttatt accagctaat aagccgacaa
    16081 agaagccata aaaaggaaat aaaagcaacc atataaaaag actgacacat ttttgggtga
    16141 ttgaaaaatt gaatgtaact aaattaaaaa ttaaaagtat aaaactaaat acaaataata
    16201 aatatcgcaa agggaatatt cccaatggct ccagtaatct tccaccacca ccaataatca
    16261 gttcaataca taaaaatgtt ataaaaattt tatttatatt attcatattc gtaaggtgat
    16321 gttttttaaa atatgtttaa aatcaacatt aataaaatat aaattttatc agcctttttc
    16381 cgccttaact tccaaaaact taaagccaag atcaaattgc ctatataacc tttacccaat
    16441 tgattattca caaagctatc atcagctaag gcattagcta ttttagttac ttcttttcga
    16501 taccacttgt ttctcaccaa accaagccgt ttgtattttg ctttaaaact aatgtttgct
    16561 cccacttggt tgttttcatg ctgtctgtac agcattgttg gttttctatc aatataccac
    16621 gaataatttc tcgtccgcgc aaatgaatat aaaaaccaat catgcagaca gatatcttgt
    16681 gcagcatttt tattatttat gatgaacttc ttaaattcaa taagcgtttc tttttttata
    16741 acatatgtac accccggacc tgctgcttca aaaaaataat cgaattgagt ttgcgactga
    16801 gccttatcaa ctaaattttt ttgccctgat ggataatatg caattacatt gctggaataa
    16861 caattcgcat tattaaatac caagcagtca attgcatttt ttattttatc tttataccaa
    16921 atatcgtcct gatcactaaa acagatataa tcatagtttt caagatcgac atctttaatt
    16981 aatctataaa aatttttacc tgccccccca aacttattga cgcttggaag tatatttata
    17041 attgagggat atgataattg aaattcttca caaatattta atgtattatc agttgataaa
    17101 tcatctgata taaaaattga gatgtctaca tctttttgtt caataatact ttttaattgt
    17161 tcctcaatcc agcattcgcc attatacgta gccataagta cagctactct tttttgatca
    17221 gaactatgca ttataacgac cgcccccagt aattcctctt attccatcca gcgtagccaa
    17281 aatatgtttt ttcatatatt caattttatt tttttgagtt gcgataatta aagtcgtatg
    17341 aattaaagaa aaaacaactt ccctaataga gagtaaaaga ggcacatgat tttttctaag
    17401 caaaagaaat gcatttctta ctcgataata acgacgtact ggcgagtgaa ttggtatgtt
    17461 aatcccacag atttttactg acttatcccc aatagcatga accatttcta tatcaggaat
    17521 gatatgaact aatatgccat agtttaagca tctaagacac cattctgtat cgacataatc
    17581 aataaaaagt tcctcatcca tcattccaac aatctcaaaa acttcttttg aaaccatggt
    17641 tccggatgag attgttactg aacttttaaa cggtgtctgt ccttcagtga catgaatttt
    17701 ctctcgaaga ccattttttt tgatattaca aataggataa taatgtgact tatctctatc
    17761 aataaaaact gggccagcta tttttgcatt ttcattatcc atatagcagg aaagtttttt
    17821 gataaactca ttgcgaatac ggctatcttg atcaaaaaaa attatcttct ctgccccttt
    17881 ttcaagagaa tatcgaatcc caatattttg tgcttttgct ataccttcat tcctgccaag
    17941 acaaattaaa tgaacttttt caatgtctgc taatttatct ttcaaggtga aattagtcct
    18001 attatcgact actacaacgt aacaattttg ctcctttagt tcagtgatca gttcattcag
    18061 atttttaagt tcgggattat atgtcacaat gacagcgtaa atattactat tcattatcca
    18121 agtgactcag taattggtta attttagttt tcatatacag aacacattct agtataaagc
    18181 cattgtaata acagagcatc taaaattaca cgtatgctcc aggatattgc ggcacccagt
    18241 aacccatatt tttcgaccat aaaatacaaa agggctaaat aaggaaccaa ttcagcacaa
    18301 tgaatcaaag cagtaatttt agattttcct aatgcctgga ttgcagagaa tggtatcatt
    18361 gccagcgcat taaagaaaaa accgatcaat aacacattta atatattaaa ggaattaatt
    18421 tggtaattct caccaaacca tattttcaat acaagccctg aatataacaa gcctatgatt
    18481 atcaccggca aacagattag aaacattaaa agcaatgatt tatttacatt acgtttaaat
    18541 tctttaaagt ctttgatgtt acttaacctt ggaaacactg ccctcccgat tgctgcaggt
    18601 attattccta attttagaat aacctccgct ggtgcagaat aaaatgcaac tttatctgcc
    18661 cccataatat ttgacactat aaatcgatca aaatacacca tgaccggact tatgatatta
    18721 cttactgtca tccaaccgcc aaagaaaaag agtcgtttaa aggttttata acaaaaatga
    18781 acccccgaaa ttttaatatc attcctgaca taataggcag agactaaaat cgaaataact
    18841 cttgcaaata ttaaaccagc aaccgctgcc gacaatgtag cactataaaa aacaaatatg
    18901 gccggaattc ccgcgatgca agagcttgat atagattttt gaatatttac aatgccaaat
    18961 ttttcatccc cctcaagaat ggctgaccat aattgattta gaataaataa tggaatgcaa
    19021 atagccaaca gtttaaatgc tagttgtata tcactatgtt caacaccaga aatttttaga
    19081 taattaacaa ttccatcaga gaaaatcaat aataaaaaag caccaaagca tgaaaatagc
    19141 accaaaaaag atgtacttgt tgaaattacc ttggttcttt catgatgatt atcacgatga
    19201 attgcaattt ctctaataac agagcgagtc aggcctacat caaaaattcc agcataccca
    19261 acaagtgcaa ttgcaattgt ataaacaccg aaaccttcag gccccaaacc tcgagcgaga
    19321 aatcctagcg aggggatagc aattaaagtt ggtattgcat agccaaaaag gttccagaca
    19381 ctatttttta ttatgctcat tattacattt ctttatcata taaaataatc agcatctaca
    19441 aatttttgac cattaatatc tttatcagaa agcaatggat tttgaaccgg ccattctata
    19501 tttatacttt tgtcgctcca taaaatccca cgctcttgca tttttgaata gaaattattt
    19561 gttttgtaga caaattctgc ttcatcacta agaacgcaaa acccatgagc gaatccttcc
    19621 ggtatccata attgacgctt attctccgcc gacaaattta caccaaccca cttgccaaat
    19681 gttggtgatg atttccgaat atcaacagct acgtcaaaaa cttcaccgac aacacagcgt
    19741 accagttttc cttgagcata aggttccagc tgatagtgca gtccgcgtaa cacaccctta
    19801 gttgatttag aatggttatc ctgaacaaac tcaacctttc gtcctacagc ctcttcgaaa
    19861 actttctgat tgaagctttc cataaaaaag ccccgctcat caccaaatac ttttggttca
    19921 aaaattagca catcaggaat ttcagtttta attacgttca ttttattaat aacctttaat
    19981 cattttcagc agatactgac cataagcatt tttcttcagc ggctcggcta ataccttcac
    20041 ttgctcagca tcaataaagc ctttacggta agcaatttct tccgggcagg aaactttcag
    20101 cccctgacgc tcttcaattg ttgcaatgaa gttgcttgct tcaataagac tttgatgtgt
    20161 ccccgtgtcc agccatgcat aaccacgccc catcatagcg acagacaaac gtccttggtc
    20221 catataaata cggttaatat cggtaatttc cagttcgcca cgtgcagatg gtttaaggtt
    20281 tttcgccatt tctacaacgt cattgtcata gaaataaagc ccagtaaccg catagttact
    20341 ttttggttcc agcggttttt cttccagact aattgccgta ccgttatcat caaattccac
    20401 cacaccgtag cgctcaggat catttacgtg ataggcaaaa accgttgcgc cactctctct
    20461 gttaacagcg gtatccatta atttcggcag gtcgtgacca tagaagatat tatcaccaag
    20521 taccagcgca caatcatcac cacctataaa ttcttcacca atgataaatg cttgcgccag
    20581 accatccgga ctcggttgta ctttatattg cagattaagc ccccactggc ttccatcacc
    20641 aagcaattgt tgaaaacgtg gtgtatcctg cggcgtactg ataatcagaa tatctcgaat
    20701 acccgccaac ataagcgttg aaagcggata ataaatcatc ggtttatcat aaatcggtag
    20761 cagttgtttg ctcacagcca ttgtcacagg ataaagacga gtgccggaac caccagccag
    20821 aataatacct ttacgcgttt tcatttcact attcctttta attcatctcg ctctggcatc
    20881 atgagcgaga tgcaataatt tgttaaattg ccgtggtcgt aaataattcg ttgagcatac
    20941 gtttcacgcc cacctgccag tcaggcaaga caagcgcaaa gttctgctga aatttttcgg
    21001 tattgaggcg agaattatgg ggacgacgtg ctggtgtggg ataggccgtt gtcggcacgg
    21061 cattaagttt gttaagagca aggttgatcc ccgctctgcg cgcttcttca aataccagag
    21121 cggcataatc gtgccaggtt gttgtaccac cagcaaccag atggtacaaa ccagcaactt
    21181 ctggtttatt tgcggccacg cgaatggcat gagcggtgca atcagccagt aattcagccc
    21241 ccgttggtgc gccgaattga tcgtttatta cagccaactc ttcgcgttct tttgccaggc
    21301 gcaacatcgt tttggcaaag ttatttcctt taccagcgta tacccagctg gtacggaaaa
    21361 taagatgctt tgcgcaatgt tcttgtaacg ctttttcccc agctagcttg gtttcaccgt
    21421 agacatttag cggtgcggtt gcatccgttt ccagccatgg cgtgtcaccg ttgcctggga
    21481 aaacataatc agtggagtag tgtataaccc aggctccgac ttcattagct gcttttgcaa
    21541 tcgcttcgac gcatgtcgcg ttaagtaatt gcgcgaaatt cggttctgat tccgctttgt
    21601 ctactgcagt gtgagcagca gcattaacaa taacatcggg gcgaattttt ttgacggttt
    21661 cagccacacc ttcggggttg ctgaaatctc cacaataatc agtggagtga acatcgagcg
    21721 caatcaagtt acccagagga gccagagcac gctgcagttc ccaacccacc tgccctgttt
    21781 tgccgaaaag caggatattc attactgacg gccctcatag ttttgttcaa tccacgactg
    21841 ataagcaccg cttttcacat tctcaaccca atttgtatta gccagatacc attccaccgt
    21901 tttacgaatc ccgctctcaa acgtttcttg cggtttccag cccaattcgc ggctaatttt
    21961 atcggcatca atggcgtaac ggcggtcatg ccccgggcga tcggcaacat aagtaatttg
    22021 ctcacgataa gatttctctt tcggtacaat ctcatccaac aaatcacaaa tagtgagcac
    22081 tacgtcgatg tttttctttt cgttatgtcc accaatgtta taagtttcac ccgctttacc
    22141 ttcggttacg acggtatata acgcacgagc atgatcctct acatacaacc agtcgcgaat
    22201 ttgatcccct ttaccataaa taggtaatgc cttaccttcc agagcattca gaataaccaa
    22261 tggaataagt ttttccggga agtgataagg accataattg ttcgagcaat tagtcacaat
    22321 ggttggtaaa ccataagtcc gcttccaggc acgtaccaaa tgatcgctgg aagctttaga
    22381 cgcagaatac gggctacttg gcgcgtaagc tgtcgtttcc gtaaatagcg gtaacgcttc
    22441 gttattattt gcttcatccg gatggggtaa gtcaccataa acttcatcag tagaaatatg
    22501 atgaaagcgg aaactttttt tcttttcatc attcagagca gaccagtaat tgcgcgccgc
    22561 ttctaaaaga acataagtcc ccacaatatt ggtttcaata aatgccgcag ggcccgttat
    22621 tgaacggtca acgtggctct ctgccgccag gtgcatcacc gcgtctggct ggtgctgtgc
    22681 gaaaatacgc gacatggcta cagcatcgca gatatctgca tgctcaaaag cataacgttc
    22741 agaatcagaa acatcagcga gtgattctag gttcccggcg tatgttaatt tatcgacatt
    22801 aacaacacta tcttgcgtat tatttattat gtgacgaaca acagcagaac caataaaccc
    22861 tgcgccacca gtaacaagta tcttcacttt tttattccat atagctatag agcatgctgt
    22921 gaaatagact gctccagatt tgattaatag aggtattaat acacgctacc gcccctggct
    22981 taacagctac cagtgcactg catgcatctc aacgatgtgg cgagcgttag ccactcgcgc
    23041 caaatccgaa aaattcaaac gttaattgtc ttaccaaaca gccgtagaaa caaggaaaat
    23101 cctggaaaaa tttgaataat atgagctatg taacttcttg tttttatagg ctttataaaa
    23161 acggcaacgg caggaaaatt caatgagcaa aacttatgcc acgaatcttc actgccgtta
    23221 taattttctt atcaaccgtt acatccggtc agattttcat tattcgctta acagcttctc
    23281 aataccttta cggaacttcg ccccttcttt caggttgcgt aggccatact tcacaaacgc
    23341 ctgcatatag cccatttttt tgccgcagtc gtaactgtcg ccggtcatca gcattgcatc
    23401 aacggattgt tttttcgcca gctcggcaat agcatcagtc agctgaatac gtccccatgc
    23461 accaggctga gtacgttcca gttccggcca aatatcggca gaaagcacat agcgacctac
    23521 ggccatgatg tctgagtcca gcgtctgcgg ctgatccggt ttttcgataa attcaacaat
    23581 acggctgact ttaccctcac ggtccagcgg ctctttagtc tggatgacgg agtattcaga
    23641 gaggtcaccc ggcatacgtt ttgccagcac ctggctgcgg cccgtttcgt tgaaacgtgc
    23701 aatcatggca gcaaggttgt aacgtagcgg gtcggcgctg gcatcgtcga tcacaacgtc
    23761 tggcagtacc acgacaaatg ggttgtcacc aatggcaggt cgcgcacaca aaatggagtg
    23821 gcctaaacct aaaggttcgc cctgacgcac gttcataatg gtcacgcccg gcggacagat
    23881 ggactgtact tccgccagca gttgacgctt cacgcgctgc tcaaggagtg attctaactc
    23941 ataagaggtg tcgaagtggt tttcgaccgc gttcttggac gcgtgagtta ccaggaggat
    24001 ttctttgatc cctgcagcca caatctcgtc aacaatgtac tgaatcattg gtttgtcgac
    24061 gattggtagc atctctttgg gtatcgcctt agtggcaggc aacatatgca tcccgagacc
    24121 cgctacagga ataactgctt ttaaattcgt cattatttca tccacctgta aaatggttgc
    24181 tgaattatag cttgttcgat ttttttcgcc agcatcaatt accctgaatt gattactgaa
    24241 ttacttgtga tgttacgccg cttcgttgtg gattgcagta gcattgttcc taagtatggc
    24301 tccatttttc caggaatggt cgcaaatcta ctccctccgt tccggcaatc taaagttaat
    24361 cttttccaca ttaacaatat ggtgattaat cctgtcgata tcgacggagc tttgccctct
    24421 ttcattcacc gcatgaacat ttgcaagaga cagcagtgtt tcttttttcg ccataaagac
    24481 gccgcgaacg tctttgcgca tgtcgaagtt catgctcaat gctggtccaa ctgaggattc
    24541 ctgcatcaca ttgatatttc gtataaaaag atgttgcggc ttgttgtgta actccagcga
    24601 ggcacgcttc atctcaatgt tagttagcga cacaaaggag acggcattcc cggcggagat
    24661 ttggatgccg cgcaatttat aagcaagatg ggtattatcc agttgaatat tattcactcg
    24721 gaaattttgc ggtatcgaga gatatttgcc tttaattacc ccatagccga ttaacatccc
    24781 ggcgctatta atcatttcaa tattatcaat cacgaaattg tcacaaccgt aaatagcgac
    24841 tgtcgcgtta tcaatgcccg ctttcttact gaaatccggc gtgatattgc gggcattgat
    24901 attacgaata acaaaatgtt taccattttc aacatggttc aactgccgac aatccgatcc
    24961 cgtgatattc gccacgacaa agtttttcac tgcctggtct tccgggtagt tgttatcata
    25021 agtgcttccc gcaaggccta tgccgatgcc ccagttgatt ttgccgttgg tacagttgat
    25081 acgctcgatg acatggtcag atatcaaaat atcactgtcg ttaattgcca cgttccattc
    25141 aatggcgtcg ccctgtaagt cgctgaactt acagttggta atgttggcac cgataatctg
    25201 gttatgaaat ccctggcgta agatggcgta attagcgtgg ctgacagtga ggttatcgat
    25261 ggtcaggttg cgcatgaccc gtttgttttt gccgccaata taaatctgca ttaccgggcc
    25321 aaagccgctc atcgccagcc ctttgatggt gcagtcagaa ccacgcacat ccagggtaat
    25381 gttatgcata ccgccgccct ctccccctgt cacctggctg ccgtcctgta agacaaatcg
    25441 ccctctgccg ttgccgcgca ggcttccaag gatgtgtaac gttttgccgg gagggatgaa
    25501 gatgccggtg ttgatattgt cacaaacgaa tccggcaggc acgacgaccg tttgcccttc
    25561 gctgaaggct tgtttaaatg aggcgatcca gtcatgtggg ttgtagtcgt taatgttaac
    25621 gctttgtcgg gcgggaagtg cgcgagcgaa aggggtatgg aggaaggcaa gcgccgagct
    25681 tgccgtcagg aaggtgcgtc gggagagtgt tttaaatggc atacgttctc ctctataaag
    25741 cctgcagcaa gctggcgagt tctcgattaa tcacctgctg gttaaaatcg tgttcgactt
    25801 tttcgcgcgc acgtttgacg accggagcca gttcgtcggt atccagttgg ctaaacgccg
    25861 ccagacgttg tgccagtgcg cgagcatcgt tctcaggcac cagccagccg gatttatcgg
    25921 cctccaccag ttcaggaatt ccgctatgca gagtagaaac caccggaata ccgaccgcca
    25981 tcgcttccat tagcgccacc ggaatgcctt ccatatcgcc atccgcgccc gttaccgatg
    26041 gcaacaggaa gacatccgcg tcgtcgagca tctccttcac ttcatagctc ggtttaaagc
    26101 ccggcatctc taccacatct tccagttgat attgttcgat gagggtgcgc aggcgtcgtt
    26161 cccacaggcc aatgccgagg atgcgatagc gaaatgccac gccctgctct ttcaactgac
    26221 ggcaggcttc aatcgccaca tgcaggcctt ttttctcggt taagcgtgcg acagatatga
    26281 tttccagcgg cgttgcgggc gctttcaccg gacgcgggct aaagcgcgtc atgtccacgc
    26341 ccatacgcga tacggcgatt ttttctctgg ggcagcccat tttttgcagc cttccggccc
    26401 acagatcgct tatcggtaac atcaggtcgc cacggcgaaa cagttgttga tattcgggag
    26461 tgtagtggtt gagcacttcc cgactggaga tatcaatacc gtggaagatg gtggcaattt
    26521 tgccgcgaat gatacccagt tcgcgtagtt ttgctgcggt tacccccgcc gggccaaaat
    26581 gagcgatgaa cacatcggcg cgaaacggtg ttgcgacctg accacaaatg gcagacaaaa
    26641 tcaggttccg cgactcggca ccatagcgtt tgaggttgag cgcatgccag gtatttttac
    26701 gatgaatgcc acgtaaggtc tggctggcgc ggtggcgcag tttcgctact ttgcctgcag
    26761 gttcgtcctg taaccagcgg gttctggcga caaggttgta tttcgtccat gccgcgtggg
    26821 tgttctctgt atcgcctttt tgcaaagcga caatctccac ttcaaagccc atatcaataa
    26881 acgcggtaat ttgattgagg acgaaggttt ccgacgacag cgggaatttc aataagaaga
    26941 agccgacctt catttcacct ccccgatgcg ctcaagcaca gactgcacca tctgcattcc
    27001 cgtctgacgt tcgcgactga cggcttcgtt aaatcgagcg ttcagcaccg gaagctggcc
    27061 taaggtatcc gcaatcatcg cttgcaggct gccgtccagt aaatgacgga tatcaatcgc
    27121 catctctggc agtcccagct gctgcataat cccggcggat ttatgttcat agttgatggc
    27181 aatcgccgga gtagcaaagt tcattgagat aatggcggag tgcaggcgcg taccgacggt
    27241 gagttcacag gctccgagaa ttttgcccat ttccagatcg ttgagttcat ccatcactac
    27301 gtggtaacgg gcaggatcgc tgatgtgctg gcgcagattg agcgccacca tgcggtcgtc
    27361 tttgttatag ctgtcgatac ccgtacaggt ggagagcgca atcacctgat agccttcatc
    27421 gagaatgcga ttgaccaccc cggcaaaggc tttttcatac gcttgttgag tggtgccgag
    27481 acgtttgtcg aacggtgcca gttcgcgcag ggtaattgcc actgttttct gttgtgcggc
    27541 aacgtccagc cagtgttgaa cggcatagct ggcggtgaag tcttcggtgt ggtgatcgac
    27601 cagccacgcg gtatcgacgc catgttccac ttttgcggtg gtgatattgc tgcgtttcat
    27661 caaatcaagg ctgaccgatt cgcgcaggat cagcgcgtcg cagtgaccaa agacatagtt
    27721 cgccagttgg ttaaattgct catcctggaa ggggccgacg ctgtgaccaa tcataaacag
    27781 cggctttttc gccataaatg tgcaaagcgc atgttcaaac tgcggcacgc cgtagagatc
    27841 gacaaaaaac gatccgccga cctgaataat ggcgtcgtaa cctgacagca ggcgcacgaa
    27901 gtcggtgaat ccctgggcga tggcgatatt gcgcagcttg ccagtgtcgg tgacgcgtga
    27961 gagcaatacc tgatgctggt agctgcgacg gaggactttt ttaacgcgcc cgacaacgcc
    28021 cgccgcgcta ttgtgttgtg tcatctgcag gaacagcgga tcgcccatta ccgggcggtt
    28081 gagcagccag gaagaactga ccggatagcg gctcatcacg tccacttcgg tatgtggatt
    28141 gaggatgttg atggcatcaa gtaagccgcg caagatggcg ctgtcgccac gattgccgca
    28201 agtgtggttg cccagaataa gtaatttcat tttttcctca taaatttgat gccaggtgag
    28261 gctgtgtttg ttgtgttcgt tgccgttttt tgcctgatgc gacgctggcg cgtcttatca
    28321 ggcctacagg tcccgagcac agaaccgtag gacggataag gcgttttacg ccgcatccgg
    28381 caaccgttgt cggaaccgaa aacagcaatt caccccgccc gtaaaagcat tttcattttt
    28441 tcgctgcgac aaaactgacg cttcacttcc accaccagcg gatggcgtga cagcacaatc
    28501 atcaccacaa acgccagcac gcccgcggct atttgcaccg ccagcagcat ccccagcgcc
    28561 agttgccctt tcagcacaat gcccagcgca taactgacca ccagcgtcgg caacgagaga
    28621 taaaacggca gccacaaact caggatgtac tggcgataac tggaaccaag aaccggttta
    28681 atcatcacga agtaactcag aatggtgttg ataatctgca ccagcaggaa accgagcgtg
    28741 acgccgattg cgcccgccat ctgcccacca ataacaatcg ccggaataaa cagaaatgtt
    28801 ttgaatacat tgaatttaaa gctgatatcg acccgcgctt tcgccatcag cagcgaacca
    28861 atcgggttac ccacggagcg cagcagaccc acaatacaca gcaattgcag caccggaata
    28921 atgctgttcc acttttcgcc gaataccagc ggtacgaaat tattcgacac caccatcagc
    28981 cccagcagcg ccggaaagtt gataatcccc actatcgaca gcagcttgta gaagttaaca
    29041 cgcagctttt cggtgtcgtc ctgaattttg gcaaacgccg gaaacaacac gcgggtgatg
    29101 attgggttca gcttcatcgg cggtacgacg gccacgttgt acgccaggtt gtatccccct
    29161 gccacgcccg cgccaagaat acgcgccagc accagcgttg aaaggttggt gttgagatag
    29221 ttgatgatgc tgtccgccgt cagccaggca ccaaaacgta agttcggtgc cacagacgcc
    29281 agcgagaagt gcagaccggg gcaataaatt ttgcgtccaa agtagccaaa cagcagtgtt
    29341 ctcaccgcac tattgaccag ataaccgagg atcgcggtca tcgccagcgg ccagaaatgg
    29401 gcgctaacta ccgtaaaagt gaagcccgcc agcaccgcgc tggtttcgat catgccgatt
    29461 ttgttgaact ccagctcctt ctgcatcaac gcgcggaact gttgcccgtg ggggatcacc
    29521 acaaacgcca gcgacaatgt tttaatcaac ggtgccaggt ccgggttatt cagcacgtcg
    29581 ccgatgacat cactcaacaa aaacaccgac acacatacca caagccccag cccgacgttc
    29641 agccagtaca gcgtggtgag ttcaaggtga ctgatttctt ttcgctgaat aatcgagtta
    29701 gcgataccga agtcagaaag cgtatctgcc agcgcgataa tcaccagcga cacagtcagc
    29761 aggccgaact ggtggttgtc gataatccgc gccagcacgg tcatctgcac cagcccgagg
    29821 ccgatgatga tcaccgtggc aatagccgac cacttcgcgc cgctgatggt tttttcacgt
    29881 aagctcatat caatatgccg ctttgttaac gaagcccttg aataccgtca ggaaaacgat
    29941 tttgatatcg aaccagacgc tccattcgcg gatgtactca aggtcgaact cgacgcgttt
    30001 ttccattttc tccagcgtgt cggtttcgcc gcgccagcca ttaatctgcg cccagccggt
    30061 aatgcccggt ttcactttat ggcgcagcat gtagccttca atgagctggc gatactgttc
    30121 gttatgcgct actgcgtgcg gacgtggacc gacaatcgac atccccccgg tcagcacatt
    30181 gataaactgc ggcaattcgt ccagcgaggt gcggcgcaga aagttcccca ctttagtgac
    30241 gcgcggatcg ttctgcgtcg cctgggtcac cactttgtcg ttctccatca ctttcatgga
    30301 acggaacttc cacactttga tcggcttgcc atccatacca tagcgagtct ggcggaaaat
    30361 aaccggccct ggtgaactga gtttcaccgc cagcgcaata cagcacagca ccggggagat
    30421 cagcagcaga ataagcgtcg ccagcacaat gtcttccgca cgttttagca ggcggttacc
    30481 ccccgaaagc ggcgtgtcat acagcggcac caccggaacg ccgttcatct cttcgagacg
    30541 tgaatggaga atgttgaagg taaagacgtc ggggatcagc agcaccgaac aggtggtatc
    30601 cgccagttga tgggccagtt ttttcactcg tgcgccgtcg cacatttgca tcgcgatata
    30661 gacgttatga atcttgcccg cttttgcatc ctcgaccagc tgttgcaggt tacccgccca
    30721 gtcgttagaa acgccgcccg gtttcgtgtc gtggtaaacg cccaccactt caaaccctaa
    30781 ccacggctga ttacggaaac tctccatcag catttgtccg gcggctaaat cccccgctac
    30841 ggcgaccatc cgcttgttgt agccatgatt acgcagccag cccgccccaa tgcgaataca
    30901 tgaacggcaa accaccagtc cgatgctggt cagcccatac cacgccagcc agattttcag
    30961 ttgcgtgtcg aaatcattgt tgaacgccac cagtccggcg ctgaaaatca cgcttaaggt
    31021 ccagttttgt agcaacaggg caaattctgt cgctgcccga acaccgcgcc atgagcgata
    31081 aaaatcggtg atgccgccca gcatctggaa caccaccagc gtaatcagcg ccaccaacag
    31141 gtgcatgtag aggaatgaca gtccgctgac ttcgcaaacc agccatagcc cggcaaacat
    31201 gatggtgata tctgaaaagc gttgcaccat agagattaac gatgcattgg ttttcgctcg
    31261 ctcgcgcttt tttagatttg tcatcgttgt tcctgttatt agccccttac ccgcagtggg
    31321 gtaagggaag atccgacatt actcgttcag caacgtcagc agagttcgcg ttcgcgcttc
    31381 catcagcggc acatcaccgc gcgattccac attcaggcgc accaccggtt cggtattgga
    31441 ggtgcgcagg ttaaagcgcc agtcggcaaa ggtcatgctg atgccatcgc tgcgatccac
    31501 cgccagcgcc tcacggctaa aatgctgttc cacgcggtta atcgcctcaa cgggttgcgc
    31561 cagtttgctg ttgatctcac cgcttgccgg aaacgccgcc atccggtcgc gcaccagttc
    31621 gcccagcgtt tttcctttca ggcacaccag ttcggcgacc agcagccacg ggatcatgcc
    31681 gctgtcgcag taagcgaaat cacggaagta atggtgggcg ctcatttcgc caccgtagat
    31741 ggcgtcttcc ttgcgcatcc gttctttaat aaaggcgtgt ccggttttcg acattaccgg
    31801 cgtgccgcct gcagcagtca ccacatcaat agtgttccag gagagacgcg gatcatggat
    31861 gatcttcgcg ccgggatttt tttcgaggaa cgcttctgcc agcaggccga caatgtagta
    31921 gccctcgata aactgccctt tttcgtcaaa cagaaaacag cggtcaaaat cgccatcaaa
    31981 ggcaatgccc atatccgcgc cgtgtttgat gaccgcatta cgggtgtcgt cgcgacattc
    32041 cggcagcagc gggttaggaa taccgttggg gaaattgccg tccggtgtgt tgtgtacttt
    32101 gattaattcc acgggcgcgc cgagggcttt aaagcgggct tcaatggcgt ccaccaccgg
    32161 acccgctgcg ccattcccgg agttgatcac cagcttgagc ggcgtgagat ttttgacatt
    32221 gatataaccg aacaggtgat caacgtaagc gtcacgcagg ttgatttgct gatagcgacc
    32281 gcgtttggtt tcatcgacgg gaggaaagtc gttggcttca gccagacgct ggacgtcgcg
    32341 cagtccggta tcaccgctga tcgggcgagc cccctcgcgc accagcttca tgccgttgta
    32401 atccatcgga ttatggctgg cagtaacttc aatgccgcca tccacgccga gatggaacgt
    32461 ggcgaaatag atctcttcgg tgccggacat accgatatcc agcacatcga cgcccgcatc
    32521 ctgtaaacct tttgccagcg ccagttttaa ggtttcgctg gtgagacgga catcaccgcc
    32581 taacacaatg gttttcggtt tgagaaattc gccataggcg cgaccaatgc gccaggcgat
    32641 atcttcattc agttcttcgc ctaatttccc gcgaatatca taggctttaa agcaggttaa
    32701 ttttttcatg tcgttaccct ttttcaggca atagttggcc ctgaccgaaa cggccaattt
    32761 tatttttgtc gttatttaca ccgcggtttc gcattcattg cctgatgcga cgtttacacc
    32821 cgtccgtagc gatccgcgaa gcgcaccaca tcatcctctt cgagataaga gccggagcgc
    32881 acttcaatta aatcgagcgg aattttcccc gggttttcca ggcagtgcgt cgcccccagc
    32941 ggaatataaa tggactcgtt ttcaccaagc agtttgatat caccatcaat ggtgactttt
    33001 gccgttcccg cgaccactac ccagtgttcc gcgcggtgat ggtgcatctg taccgacaaa
    33061 ccctcgcccg gtttcacggt gatgcgtttc acctggtagc ggtcgcccgc gtcgatagag
    33121 tcatatttgc cccacggacg atacacttcg cgatgcaccc gatgctcatg gtgaccatcg
    33181 gctttgatct gctcgaccac ttttttcaca tcctgtaccg cgttacggtc ggcaatcagc
    33241 accgcatctt tggtctgcac cactaccaga tctttcaccc cgacggtggt gactaggcct
    33301 gattcggcat acacatagct gttttcagtt ttgtgattaa tcacatcgcc gtggcaaacg
    33361 ttgccctcgg cggtgtgggc gctgatctcc cataatgaag accaggaacc aacatcgctc
    33421 cagcccgcat ccatcggcac cacaacagca tctgccgtac gttccatgac cgcgtaatcc
    33481 accgactctt ccgggcaggc gagaaacgct tcttcatcca cacgaataaa atcgagatcc
    33541 ggatcgacgg cgctcatcgc tttttcgcag gcatcgagga tatccgggcg atattttttc
    33601 agttcttcga gatagcgtcc ggcgcggaac aggaacatgc cgctgttcca gtaatattcg
    33661 ctgcttgcta cataggcctg ggcggtttcc agattcggtt tttcgacaaa ctgcgccact
    33721 tcaaaggcca ccgtatcctg ctcacccgca gacacttcac cgcgacgaat atagccataa
    33781 ccggtttctg gtagatccgg cacaatgcca aaggtcacca gcttgcccgc ttcggcataa
    33841 ggcatggcat tacgcacggc ggcacggaac gcgtctttat cggcaatcac atgatccgcc
    33901 gccaatacca gcattaacgg gtcgctctcc gggctatgac gttttgccgc cagcgccgcc
    33961 agtgcaatgg caggtgcagt gttacgccct gccggttcga gaataatgtt ctcggtgagt
    34021 ttgttcagtt gacgcagctg ttccgcgaca ataaagcggt gctgctcatt gcaaatcacc
    34081 accgggcttt cgcactccac gccgttcagg cggcagatgg tggtttgcag catggtgaga
    34141 tcgcctttca ggcataaaaa ctgcttgggg taaagtacgc gggaaagcgg ccataaacgg
    34201 ctaccggagc cacctgccat cacaactgga tagagtttcg actgcgccat aattatcccc
    34261 gaatatcatt tataaattga cgtaacacgt tctctttatc gagcgtgcgt tcggcatatt
    34321 cacgtgccac cgtgttatgt ttgggcagca ggagcgcctg acgaatcccc gccaccagcg
    34381 cctcgaccga ttccggttct acgcaaaccg caatacccgg aaaggtttcg caaagctgcc
    34441 ccagttctgt gtgggcctct gcggtaatca ccgcgttacc gcctaccgcc agaatattgg
    34501 tcagtttcga cggcaatacg gcatctgccg cgccgcgttt ttgcaccacc agatggcaat
    34561 cgcccatctt cagcagtgcg ggtaaagcgt catacgattg cagcgggaaa aattgcatgt
    34621 tgcgcagtcc acgctgctgc gccatttttt ccagccgcgc tttgccgccg ccctgcccga
    34681 caatggcaaa aagcagcggt tcatcgcgca ggagatcggc agcttcaata acgttttcca
    34741 gcccctgctt ttcaccaata ttgccggagt aaagaatgat ttttttgtta tccggcaggc
    34801 caagctggtt acgaagggca tcaacatcgg cgtctgcaac atgctgaaaa cgggcaattt
    34861 ccgaccagtt ggggaagaag atgacgttat ccgccgccac gcctttttcg atggctttat
    34921 tcatcatcga acgcgaaatc gtggaaacgt tatcgacgtt atgcagtccg ctacgttcga
    34981 acgccgttgc cagctgtgcc actttgccgc ctttgccttt tccggcaagg cccagcccca
    35041 gcatggcgtc cacttcgtaa tcctgaatat gcagcacggt acgcgcacca gagagcttcg
    35101 ccagcaggcg cattcccggc gtgcaaaaca gcgttggcac tacgccaata atgcgatccg
    35161 gcttccagcg acgttgcgcc atcagtggaa agaaactgct gacggcaaaa ctgccgagat
    35221 gcaacaagcg tttcagggtg ctcggctgtt ttggcacgta cagcgggcag cgccacaccg
    35281 tggcggcccc ttcttctcgt ttgtagcgcc aggcggaata gttctcaccc acctgccatt
    35341 gcgggtagta aggcggtgcg gtaatgaccc gcacctcatg accttgtgcc gccaaccatt
    35401 ccaccatctc gccggtgtat ttgccgatgc cggttaactc cggcgagtag ttaatgccgt
    35461 agaccagtat tttcataatc cgggtactcc ggcacgcttc tcagcgagaa aataggcgcg
    35521 gctgttagcg tgaacatcgt tactggcgag cagcgcgtcc ggcgtcagcc agcggtaatc
    35581 gtcatgctgc tcatccggca gtaacagctc ttcttccgct actctgaagc gaaaaccgag
    35641 caccacatag tgagtggtga aatccgtgcc ggagaagtta tcgtcataaa aatgctgcca
    35701 gacaccgtaa aactggcctg ctgttatcgg cagacgcagc cccagttccg ccatcgtcag
    35761 ccgctcaaat gcggcttcca gcgtttcgtc tttctgcacg cgccctcccg gcacaaacca
    35821 gtaaccctgc gccgggcggt tggttctttt gccaagcaga aactcgccgc gactgttctc
    35881 gacaataaag tcgagagaga caagcggtgt ggagcgcact acagtggcaa agtcttcctg
    35941 acgtaaaaac atcattcccc ccgaaagcgg tcttgattct caaggaacca ctggtaagtg
    36001 ctggcaagcc ccgcttccag tgagatttcg tgataccagc caagctgatg caggcgcgtc
    36061 acatccagca gtttgcgcgg cgtaccatcc ggtttgctgg catcaaaaac cacccgacct
    36121 ttgtaaccca ccactttggc gatggtttgc gccagctcgc ggatagtgca gtcaacgccc
    36181 gtgccgacgt taatgtgcga cagcatcggt tgggtattct ccagccagac ttcatgcgcc
    36241 agctccatga catgaatgct cgccgccgcc atatcatcga cgtgcaggaa ttcacgcatc
    36301 ggtgtaccgc tgccccatac caccacgtcc ggcgcattct gtgccgtcgc ctcgtggaag
    36361 cgacgcagca atgctggtat cacatgggag ttactcgggt ggaagttgtc atgcggcccg
    36421 tacaggttgg tcggcatgac agagcggtaa tcgcgtccat actggcggtt gtaagattcg
    36481 cacagtttga tcccggcgat tttggcaatg gcgtaaggct cgttggtcgg ctccagcgtg
    36541 ccctgcaata actcgctttc tgccatcggc tgttttgcca gtttcgggta gatgcaggac
    36601 gatccgagaa acatcagttt gttcacgtcg ttctgatgcg cggcgtgaat gatgttgctc
    36661 tcaatcatca tgttctggta gatgaaatcc gccggatagg tgttgttggc aacaatgccg
    36721 cccactttcg ccgccgccag atagacctgg tcaatgcgtt cgctggcaaa gaaatcatgc
    36781 acggcgcggc tgtccagcag gttcagttca tcgcgggtac gtaataccag ctccacatca
    36841 ccgcgctgtt cgagctgccg cttgatggca gaaccgacca tcccacaatg gccagcgata
    36901 aaaatgcgtt gtttacttat gcttatgact ccagcgcgat cgccacgtcg tagccgtgag
    36961 atttcagcag agagtgtttt ttcgccgctt cgaggtcatt agccaccatt tcagacacca
    37021 tctctctgag ggtgatttcc ggtttccagc ccagtttttc gtgcgctttg gtcgggtcgc
    37081 cgagcagcgt ttcaacttct gccggacgga agtaacgcgg atcaacggcg ataatcacat
    37141 cacccggttt aacgcccggc gcgtcatgcc cggtgacgga aaccacaatg cccttctctt
    37201 caacacccgt gccttcaaag cgcagtttga tgcccagctg tgctgccgcc atttccacaa
    37261 actgacgtac ggagtactga acgccggtcg cgataacgaa atcttccggc tgttcctgtt
    37321 gcagcatcat ccactgcatt tttacgtagt ctttggcgtg gccccagtca cgcagggaat
    37381 ccatattgcc gaggtacagg cacgactcca gcccctgggc gatgttggcg attgcgcggg
    37441 tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga ttcatggttg aagagaattc
    37501 cgttacaagc gtacatgccg taggattcgc ggtagttaac ggtgatccag taggcgtaca
    37561 gtttggcgac cgcatacgga gatcgcgggt agaacggcgt ggtttctttc tgcggaattt
    37621 cctgcaccag accatacagt tcagaggtgg aagcctgata gaaacgagtt ttcttttcca
    37681 gaccgaggaa gcggatcgcc tccagcaggc gcagcgtacc catcgcgtcg acgtcagcgg
    37741 tatattctgg tgactcaaaa gagaccgcaa cgtggctcat tgcgcccagg ttgtacactt
    37801 catccggctg tacttcacgc aaaatgcgcg tcaggttaga ggtatcactc aggtcgccat
    37861 aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg ataaatgtga tccacgcgct
    37921 cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac ctcgtaacct ttttccagca
    37981 gaaactctgc caggtaagaa ccgtcttgtc cggttacacc ggtgatgaga gcgacttttg
    38041 acatgtctta ttcctctgta tttttgaatt tattcagttt caacgcgttc gcgtatcacc
    38101 actgcgggat tcccccggca aaccacattt gccggaagcg atttaaaaac actgcttcgt
    38161 gcacccacga cggtgccgtc gccgattgtg acgcctgggg caacaaagac atcggttgcc
    38221 agccagcatt tctcgccaat cacaataggc gtggcgttaa tggtgaaatg ttgacttgca
    38281 tggtcgtggc taccggtgca taaataactt ttttgcgata tcaccgaatg tgcgccaatg
    38341 gttatttcac cgagggtata taaattgacg tcatcgccaa cccacgcgta atcacctaag
    38401 gttaatttcc acggataggt aatttttact gacggacgaa taactacgtt ttttcctatt
    38461 tttgctccga ataaacgtaa taaaaaagcc cgccagcgat acaatacttg tggcgaccag
    38521 gcaaatattg ttgcctgtac tgcccaccat aattgcactt taatagcgtt gccgccccgg
    38581 aaccctttcg gcaccgagaa accgcttaaa tcttgcattg gttttcctta tattcagact
    38641 ttgttatata aggctttagt cttacctgtc gtacgttggc gtaaatgcca ggataattct
    38701 gcccagaagc caggtacatg taatatctgc cgttggactt ttttggcgtc agca
//
LOCUS       DAJTYF010000022        37647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTYF010000022 DAJTYF010000000
VERSION     DAJTYF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.022"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..175)
                     /locus_tag="OO590_001298"
     CDS             complement(<1..175)
                     /locus_tag="OO590_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6636733.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO590_001299"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO590_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6636734.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO590_001300"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO590_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6636735.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO590_001301"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO590_001301"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6636736.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO590_001302"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO590_001302"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6636737.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO590_001303"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO590_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6636738.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="OO590_001304"
     CDS             complement(5179..5421)
                     /locus_tag="OO590_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6636739.1"
                     /translation="MDTSFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO590_001305"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO590_001305"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6636740.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHIEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO590_001306"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO590_001306"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6636741.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="OO590_001307"
     CDS             complement(8338..9312)
                     /locus_tag="OO590_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6636742.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="OO590_001308"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="OO590_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6636743.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="OO590_001309"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="OO590_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6636744.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="OO590_001310"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="OO590_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6636745.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="OO590_001311"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="OO590_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6636746.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="OO590_001312"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="OO590_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6636747.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="OO590_001313"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="OO590_001313"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6636748.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="OO590_001314"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="OO590_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6636749.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVDGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="OO590_001315"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="OO590_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6636750.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFSYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO590_001316"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO590_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6636751.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="OO590_001317"
     CDS             23179..23382
                     /locus_tag="OO590_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6636752.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="OO590_001318"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="OO590_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6636753.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="OO590_001319"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="OO590_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6636754.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="OO590_001320"
     CDS             26387..26659
                     /locus_tag="OO590_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6636755.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTLGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO590_001321"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO590_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6636756.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO590_001322"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO590_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6636757.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="OO590_001323"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="OO590_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6636758.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO590_001324"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO590_001324"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6636759.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31772)
                     /gene="degQ"
                     /locus_tag="OO590_001325"
     CDS             complement(30405..31772)
                     /gene="degQ"
                     /locus_tag="OO590_001325"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR6636760.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDEKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTNTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31926..32324)
                     /locus_tag="OO590_001326"
     CDS             complement(31926..32324)
                     /locus_tag="OO590_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6636761.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32518..33645
                     /gene="zapE"
                     /locus_tag="OO590_001327"
     CDS             32518..33645
                     /gene="zapE"
                     /locus_tag="OO590_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6636762.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33864..34292
                     /gene="rplM"
                     /locus_tag="OO590_001328"
     CDS             33864..34292
                     /gene="rplM"
                     /locus_tag="OO590_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6636763.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34308..34700
                     /gene="rpsI"
                     /locus_tag="OO590_001329"
     CDS             34308..34700
                     /gene="rpsI"
                     /locus_tag="OO590_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6636764.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35095..35733
                     /gene="sspA"
                     /locus_tag="OO590_001330"
     CDS             35095..35733
                     /gene="sspA"
                     /locus_tag="OO590_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6636765.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35739..36236
                     /gene="sspB"
                     /locus_tag="OO590_001331"
     CDS             35739..36236
                     /gene="sspB"
                     /locus_tag="OO590_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6636766.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36279..37646)
                     /gene="dcuC"
                     /locus_tag="OO590_001332"
     CDS             complement(36279..37646)
                     /gene="dcuC"
                     /locus_tag="OO590_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6636767.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9115 a         9415 c         9904 g         9213 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 accaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgtggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atagatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcaa accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaagggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccctatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagc gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggttaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaaccggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcggttt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatccgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gctaggtgca taccgaacat caggattgcg actacaatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagcgtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcatctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc
     5401 ctgaacaaaa ctagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg atgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taactttgta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgcgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaagataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccttggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtgcttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 caggactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat acggctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctccc cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgactttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgcc
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcata
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgcc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcactgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttgacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gatatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag agcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc gggcagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttgacgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacctggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacatt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaagct cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgtcga gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggatgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttagt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg acgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactcta accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac cggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt aatgagattg agagtcagga ggatttttat cgtgacttta gccaaaccct
    26461 tggtctggct aaagataagg tacgcgatct tgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccccgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagcatacac taattattga
    27061 actaattaat accacgctaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcgaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggcgtg
    30661 atcatttcag cgctggccga cgaagaggtg ttggtatcaa gcgtcacttc tacttccagt
    30721 ggtttgccgt tacgcagcag gccaagcttc actttcgtgc ccggctctgt agtcgcgata
    30781 cgagagcgca actcagcaaa gctattcagc ggtttgccgt tgaggctggt aataatatcg
    30841 cccgctttga cgcccgcttt cgctgagcca gaacttggca acacttcgct gacaaacgcg
    30901 ccacgctgca cgtcaaggtt gaaggctttg gcgatatcgg cactcatctc ggtgcctttg
    30961 atgcctaaca aaccgcgttt gatttcacca aagtcgataa gctgctgcgc cagtgttcgc
    31021 gccatattac tggggatggc aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt
    31081 gcagtgttga tgccaattaa ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg
    31141 cggttaatgg aagcatctgt ctggataaag ttttccagac cttcaagatt caacccgctg
    31201 cggcctaatg cggaaacaat gccagaggtg gcggtttgcc caaggccaaa tgggttaccg
    31261 accgctacgg caaaatcacc gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt
    31321 aatttgctcg ggttttgaat ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc
    31381 agtttttcat caaactcgcg cccatcattg agctgaatac tgattttctg tgcctgatta
    31441 atcacatggt tgttggtcag cacatagcct ttactggcgt tgatgatgac accggaacct
    31501 aaaccttcga agggttgtgc aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat
    31561 tcttccggga ttttctgtcc ctgactggcc gttccttcca cccgtacgct caccactgcc
    31621 ggaagcactt tttccagcat tggagccaga ctggggagag gggcctgatc ggcaacctgg
    31681 cctggaatcg acgcgacggc ctgaaatgac gccgagagag ttaacccggc acttaacgct
    31741 aatgcactca acagctgggt ttgttttttc attattcctg ctctcgtacc tgaatgataa
    31801 gaaaagagat tcaaaacagt ttgatgttat tgaattttca gacggctaac aatgagacac
    31861 cagattaaat gatagtcgtg gagggagaga agaggagggc gcaatggctg cgcccgaaaa
    31921 ataaattagt cgcgcttcgc gccagtacgc agcaggccgg atgcgccttc agaatagtcg
    31981 cgaggcatct gcaccggtgc ctgatcgttg ctggcttcag actctgccag acgattacgg
    32041 aacgggtttg cttcagcaga cagttccggc agcaggctgc tggagctttt tgccatgtgc
    32101 tgatacagct ggcgatagtc gtgcgccatg gtatccagta attccgcgct gcgggcaaag
    32161 tggctaacca gctcttcgcg atactcgtcc agttcagctt tattcttttc cagttcgtac
    32221 tgcaacgcct gttgctggcg tagtttacga ttaccaaaac gcatggccac agcaccaata
    32281 atgatgccga cgactaaccc aattagcgca tattcccagg tcatgaacat ctcccgttgt
    32341 cttttgtttc cgtagggtgt tggcttcagg ctccatgcct gcggctgatt atgccactat
    32401 aaccgttaat tccacagaag tggaatcccg actgcatatc gcgtagtgta gaacggcctt
    32461 tttttcgtca accgtgaaca acggcgcacc gattattcaa ggaataacaa taagatcatg
    32521 caaagcgtta ccccaacatc gcaatacctg aaggcgctca atgaaggcag ccatcaaccc
    32581 gacgacgttc aaaaagaggc cgtcagccgc ctggaaatta tttatcaaga actcatcaat
    32641 agcaggccac cagcccccag gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt
    32701 aaacgcgaag acacaaagca tacgccagtg cgtggcttat atatgtgggg cggtgtagga
    32761 cgcgggaaaa cctggctgat ggaccttttc tatcaaagcc tgccgggaga gcggaaacag
    32821 cgcctgcact ttcaccgttt tatgctgcgg gtgcacgaag agctaactgc cttacagggg
    32881 cagaccgatc cgctggaaat tattgccgat cgctttaaag ccgaaactga cgtgctctgt
    32941 tttgacgaat tttttgtttc tgatattacc gacgccatgc tacttggcgg tctgatgaaa
    33001 gccctgttca cccgcggtat taccctggta gcgacgtcaa atattccgcc ggacgaactt
    33061 tatcgaaatg gcctgcaacg tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat
    33121 tgtgatgtaa tgaacgtgga cgctggtgtt gattatcgac tgcgtacact cactcaggcg
    33181 catctgtggc tttcgccact caacgatgaa acccgggcgc agatggataa actatggttg
    33241 gcgctggcgg gggtgaaacg agaaaattca ccgacgttag aaatcaacca tcggccattg
    33301 gcgacaatgg gcgtcgagaa ccagacgctg gcggtttctt ttactacgct gtgcgtcgac
    33361 gcccgcagtc agcatgacta tattgcgctc tcacgcctgt ttcacacagt aatgttgttt
    33421 gatgtgccag tcatgacgcg gttgatggag agcgaagcgc ggcgctttat tgcgctggtg
    33481 gatgagtttt acgagcgcca tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa
    33541 atttatcagg gtgagcggct gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag
    33601 atgcaaagcg aagagtatct gaagcgcgag catttggcgg gttaaaacct gccacaaatc
    33661 acaaaaaggg gtcgatcttt gaccccgact tctctataat cctgcgaccc cacgttacaa
    33721 gaaagttttt ttcccaaaac tttttgtgtg ctggcatagg ctattcgaag gggtaggttt
    33781 gccggacttt gtcgtgtgaa cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta
    33841 actatttatt gggtaagctt ttaatgaaaa cttttacagc taaaccagaa accgtaaaac
    33901 gcgactggta tgttgttgac gcgaccggta aaactctggg ccgtctggct actgaactgg
    33961 ctcgtcgcct gcgtggtaag cacaaagcgg aatatactcc gcacgtagat accggtgatt
    34021 acatcatcgt tctgaacgct gacaaagttg ctgtaaccgg caacaagcgt actgacaaag
    34081 tgtactatca ccacaccggc cacatcggtg gtatcaaaca agcgaccttt gaagagatga
    34141 ttgctcgccg tcctgagcgt gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc
    34201 cgctgggtcg tgctatgttc cgtaaactga aagtttacgc gggtaacgag cacaaccacg
    34261 cggcacagca accgcaagtt cttgacatct aatcgggatt ataggcaatg gctgaaaatc
    34321 aatactacgg cactggtcgc cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca
    34381 acggtaaaat cgtaatcaac caacgttctc tggaacagta cttcggtcgt gaaactgccc
    34441 gcatggtagt tcgtcagccg ctggaactgg tcgacatggt tgagaaactg gacctgtaca
    34501 tcaccgttaa aggtggtggt atctctggtc aggctggtgc gatccgtcac ggtatcaccc
    34561 gcgctctgat ggaatacgac gagtccctgc gttctgaact gcgtaaagct ggcttcgtta
    34621 ctcgtgacgc tcgtcaggtt gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc
    34681 cgcagttctc caaacgttaa ttggcttctg ctccggcaga aaacaatttg cgaaaaaacc
    34741 cgcttcggcg ggttttttta tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct
    34801 ttttgctgcc gtctgaaaga caggtaaaac aaaaaccaca tcgccaataa gggtctaagt
    34861 caactatttc agactaaagc gcatctcttt ttccccattt ccggcatcca ctcaccacaa
    34921 aggtcgcaaa atctggtaaa ctatcatcca attttctgcc caaatgtcgg gtattgctca
    34981 ttttttgttt gattttcgaa caaagagagt cgttccttat tgggtaacac aacttctgac
    35041 tggccacctg gtggctggta gcagtaaaaa ttctgactat acctggaggt tttcatggct
    35101 gtcgctgcca acaaacgttc ggtaatgacg ctgttttccg gtcctactga catctatagc
    35161 catcaggtcc gcattgtgct ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa
    35221 aaggacaatc cgcctcagga tctgattgac cttaacccga atcagagcgt tccgaccctg
    35281 gtggatcgtg agctgaccct gtgggaatct cgcatcatta tggaatatct ggatgagcgt
    35341 ttcccgcatc cgccactgat gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac
    35401 atgcatcgca tcgaaaaaga ctggtacacg ctgataaaca ctatcatcaa cggttcagct
    35461 tctgaagcag atgccgcacg taagcaactg cgcgaagaac tgctggcgat tgcgccggtc
    35521 ttcggtcaga agccgtactt cctgagcgat gagttcagcc tggtcgattg ctaccttgct
    35581 ccgctgctgt ggcgtctgcc gcaactgggc atcgagttca gcggcccggg tgcgaaagag
    35641 ctgaaaggct atatgacccg cgtctttgag cgtgactctt tccttgcttc tttaactgaa
    35701 gcagaacgtg aaatgcgtct gggccggagt taatctgtat ggatttgtca cagctaacac
    35761 cacgtcgtcc ctatctgctg cgtgcattct atgagtggtt gctggataac cagctcacgc
    35821 cgcacttggt ggtggatgtg acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg
    35881 acgggcaaat cgtactcaac attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg
    35941 atgaggtgcg ctttaacgcg cgctttggtg gcattccgcg tcaggtttct gtgccgctgg
    36001 ctgccgtgct ggctatctac gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag
    36061 ctgcctacga tgaagatacc agcatcatga atgatgaaga ggcatcggca gacaacgaaa
    36121 ccgttatgtc ggttattgat ggcgacaagc cagatcacga tgatgacact catcctgacg
    36181 atgaacctcc gcagccacca cgcggtggtc gaccggcatt acgcgttgtg aagtaataca
    36241 aaacaggccc aggcggcctg ttttgtcttt ttaatgactt aataaaatag cgtgattgtg
    36301 gcaatcatat tcaccacgaa accgactgcc atggggatcg ctgtccgctt caccaccgca
    36361 aaaggcgaaa cgcctgcaat tcctgacgta acgaccacca ccgcagtaat cggcgaaacc
    36421 gcgcgcgcta gcgtcgtggc aaaatgcatc ggcataatca ttacaaccgc tggtacatgt
    36481 agtccggctg cgatattcgg aataagactg gcaaatgaca taaacggcgc attgccagag
    36541 cccatcacaa tggcacaaat ggcaatgact agcgccataa taatcatcac gccaataccg
    36601 cccagaccag aatgctccgc acccctgata accgcatcaa cagtgccaat cgtcgttaag
    36661 cctttcgcaa atatttcacc cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc
    36721 atgccgtcaa aaaacgcctg cacatcgtcc attgtttcgc gcaagttatg cttgcggaag
    36781 aactcaacaa acatcgtcac cgtcaaactt agtaacatca ccaccaccag atgcagttct
    36841 gacttcatca gcccgacgtg ggcgaggaac agcgagccga gcatcaggat taacggcatc
    36901 acaggtaaaa tggcgtaata gagcggcggg acattatcga gagttttttg ctctgcctgt
    36961 tcgtgattga tatttttatc ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc
    37021 gccaccgaga taatgacgca agaggcgacc ggaagctggt agtggaagaa gtatgtggca
    37081 attttcattc ccgcgacctg ggctgcaaaa atggagttcg tttccagaat cccccactca
    37141 atggacatcg tggttgcgat aaccgccacc gcagagaggc gacttactcc cagactcacc
    37201 agcgtcggaa ataaggtgac catcagcaac atacccagac cggaggcgct ggtaataaac
    37261 tgcgccatga tttggccgat gacgtaagtt gccgacagaa taatatacgg cgagcgaatg
    37321 agttttaacg ggcggcttag caagctcacc atcgcgcgac tggctcctat gcgctccatg
    37381 tagcgggcat aaccgcccac cgccataatc gacagcccca atccggcaat gcggttggac
    37441 agcatgcgca ggatttcatt ataaatgtcg acgatcaagt agccgctgct tttggcggga
    37501 tcgagtacac caccgaaccc taaccagact ccgcacatca tcaggaagac acctgcggca
    37561 gccagcacca cctgaggttt gtagtttttc aggatcaaat agcccatcgt aattaatacg
    37621 atgacagata taattatgcc gaacatt
//
LOCUS       DAJTYF010000023        36606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTYF010000023 DAJTYF010000000
VERSION     DAJTYF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.023"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..168)
                     /gene="ygdR"
                     /locus_tag="OO590_001333"
     CDS             complement(<1..168)
                     /gene="ygdR"
                     /locus_tag="OO590_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6636768.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQG"
     gene            complement(306..1019)
                     /locus_tag="OO590_001334"
     CDS             complement(306..1019)
                     /locus_tag="OO590_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6636769.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            complement(1088..1777)
                     /gene="mutH"
                     /locus_tag="OO590_001335"
     CDS             complement(1088..1777)
                     /gene="mutH"
                     /locus_tag="OO590_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6636770.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            2462..2992
                     /gene="rppH"
                     /locus_tag="OO590_001336"
     CDS             2462..2992
                     /gene="rppH"
                     /locus_tag="OO590_001336"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6636771.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            3005..5251
                     /gene="ptsP"
                     /locus_tag="OO590_001337"
     CDS             3005..5251
                     /gene="ptsP"
                     /locus_tag="OO590_001337"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6636772.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            5402..6277
                     /gene="lgt"
                     /locus_tag="OO590_001338"
     CDS             5402..6277
                     /gene="lgt"
                     /locus_tag="OO590_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6636773.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINSELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            6284..7078
                     /gene="thyA"
                     /locus_tag="OO590_001339"
     CDS             6284..7078
                     /gene="thyA"
                     /locus_tag="OO590_001339"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6636774.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            7262..7732
                     /locus_tag="OO590_001340"
                     /pseudo
     CDS             7262..7732
                     /locus_tag="OO590_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            7723..8286
                     /locus_tag="OO590_001341"
     CDS             7723..8286
                     /locus_tag="OO590_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6636775.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            8283..8690
                     /locus_tag="OO590_001342"
     CDS             8283..8690
                     /locus_tag="OO590_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6636776.1"
                     /translation="MNREKGVSSQALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            8675..8998
                     /locus_tag="OO590_001343"
     CDS             8675..8998
                     /locus_tag="OO590_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6636777.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPLANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            9011..12379
                     /gene="recC"
                     /locus_tag="OO590_001344"
     CDS             9011..12379
                     /gene="recC"
                     /locus_tag="OO590_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6636778.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            12555..15443
                     /gene="ptrA"
                     /locus_tag="OO590_001345"
     CDS             12555..15443
                     /gene="ptrA"
                     /locus_tag="OO590_001345"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6636779.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRMADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            15436..18978
                     /gene="recB"
                     /locus_tag="OO590_001346"
     CDS             15436..18978
                     /gene="recB"
                     /locus_tag="OO590_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6636780.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            18978..20804
                     /gene="recD"
                     /locus_tag="OO590_001347"
     CDS             18978..20804
                     /gene="recD"
                     /locus_tag="OO590_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6636781.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(20866..22197)
                     /gene="argA"
                     /locus_tag="OO590_001348"
     CDS             complement(20866..22197)
                     /gene="argA"
                     /locus_tag="OO590_001348"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6636782.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            22429..23682
                     /gene="amiC"
                     /locus_tag="OO590_001349"
     CDS             22429..23682
                     /gene="amiC"
                     /locus_tag="OO590_001349"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6636783.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(23757..23833)
                     /locus_tag="OO590_001350"
     tRNA            complement(23757..23833)
                     /locus_tag="OO590_001350"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23797..23799),aa:Met,seq:cat)
     gene            complement(23867..23943)
                     /locus_tag="OO590_001351"
     tRNA            complement(23867..23943)
                     /locus_tag="OO590_001351"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23907..23909),aa:Met,seq:cat)
     gene            24152..25249
                     /gene="mltA"
                     /locus_tag="OO590_001352"
     CDS             24152..25249
                     /gene="mltA"
                     /locus_tag="OO590_001352"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6636784.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            25488..26294
                     /gene="tcdA"
                     /locus_tag="OO590_001353"
     CDS             25488..26294
                     /gene="tcdA"
                     /locus_tag="OO590_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6636785.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(26345..26788)
                     /gene="csdE"
                     /locus_tag="OO590_001354"
     CDS             complement(26345..26788)
                     /gene="csdE"
                     /locus_tag="OO590_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6636786.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(26788..27993)
                     /gene="csdA"
                     /locus_tag="OO590_001355"
     CDS             complement(26788..27993)
                     /gene="csdA"
                     /locus_tag="OO590_001355"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6636787.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            28185..28412
                     /locus_tag="OO590_001356"
     CDS             28185..28412
                     /locus_tag="OO590_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6636788.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            28763..29680
                     /gene="gcvA"
                     /locus_tag="OO590_001357"
     CDS             28763..29680
                     /gene="gcvA"
                     /locus_tag="OO590_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6636789.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            29699..30094
                     /locus_tag="OO590_001358"
     CDS             29699..30094
                     /locus_tag="OO590_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6636790.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            30087..31187
                     /gene="rlmM"
                     /locus_tag="OO590_001359"
     CDS             30087..31187
                     /gene="rlmM"
                     /locus_tag="OO590_001359"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6636791.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(31231..31962)
                     /gene="fucR"
                     /locus_tag="OO590_001360"
     CDS             complement(31231..31962)
                     /gene="fucR"
                     /locus_tag="OO590_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6636792.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSSALFHIRLKSHYAHKADIAREALAWIEERMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(32020..32442)
                     /gene="fucU"
                     /locus_tag="OO590_001361"
     CDS             complement(32020..32442)
                     /gene="fucU"
                     /locus_tag="OO590_001361"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6636793.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(32444..33863)
                     /gene="fucK"
                     /locus_tag="OO590_001362"
                     /pseudo
     CDS             complement(32444..33863)
                     /gene="fucK"
                     /locus_tag="OO590_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(33972..35747)
                     /gene="fucI"
                     /locus_tag="OO590_001363"
     CDS             complement(33972..35747)
                     /gene="fucI"
                     /locus_tag="OO590_001363"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6636794.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(35780..>36606)
                     /gene="fucP"
                     /locus_tag="OO590_001364"
     CDS             complement(35780..>36606)
                     /gene="fucP"
                     /locus_tag="OO590_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6636795.1"
                     /translation="GAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSV
                     QTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLA
                     QFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAP
                     HKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKY
                     GSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         8704 a         9137 c         9923 g         8842 t
ORIGIN      
        1 accttgctga tcgtgataac tcaccagccc ggtatcatca tcaatttcag gttttccatc
       61 ggtcaaaatc atacggccat ctttggtcgc catgacgtaa tcactggaac acccggaaac
      121 agcaaacgcc agtccgactg cagaaattat tactgcccat tttttcatcc ggttatcctc
      181 atttggccta cctatgataa tagtctgttt ataaatcgac gttatatatc ttgaaagcag
      241 gaaaatctta aactgtgagg gtaaaaggag tgagtacgcc ctcccgcagg agagcgtacg
      301 gagtatcaga gcggattctt tttgttgcga atcaagttga ggctttcaac cgcaatagag
      361 aagaacatcg cgaagtagat gtaacctttc ggtacgtgga tgtcgaaact ttccagaatc
      421 agggtaaagc ccaccagaat caggaaagag agcgccagca tttttaccga aggatggcgt
      481 tcgacaaaat caccaatcga gcgcgcggcg aacatcatca cgcctacggc aatcaccacg
      541 gctgccatca taataaacag gtgatctgac agacccacag cggtaatcac cgagtcgagg
      601 ctgaagataa tatccagcag cataatctgc acgatagcgc cgaggaatga tgaaacgcgt
      661 gttttcagcc cttcttcttc accttcaatg gattcgtgga tttccttgct ggctttccag
      721 ataaggaaca agccacccag aagcagaatc aaatcacggg cagaaatttc ctgactgaat
      781 attgtaaaaa gcggatttgt caggcgcgta acccaagaga ttgatgccag cagagccaga
      841 cgcataacca tggctcccgc caaccccaga cggcgcgcat gagcacgttg tgctgtggga
      901 agctttgcca ccaccagaga aaggaaaata atattgtcga tcccaagaac gatctccagc
      961 agcgtcagcg taccgagcgc aagccaggcg ttaggatcgg ttatccatgc aaataacatc
     1021 taaaaagccc tgccaaaaaa atgaagcggt aattatatgc ccggaaagcg gcaggtcaaa
     1081 gcgatggcta ctggatcaga aaatgacggg ccagtagtgc actggtgaaa ttcttcttca
     1141 aataaaagcc gcgtggcagc gtcagaatcc gttcgccccg ggcaccaatg gcttcggtaa
     1201 gcgctttcgc attcgctgct ttcggtctta tctgtaaata ttccccgtga cgagcggtga
     1261 tccgctcaac ctgaccgaga acaatcatat ccattaattc ttcccagtct tcgcgtagct
     1321 gtcggtcttc ctcttcattc gggctccaca gcaacggtga tcctacgcga cgctgcgcca
     1381 gcgggatgct gcgctcgcct tcaaccggta tccacagtac gcgtttgagc ttgtggcgca
     1441 catggctggt ttcccaggtc accccgctat tacccgttaa cggggcaaca caaacgaatg
     1501 ttgtttccag cggacgacca agactatcca cagggatagt tttaagttcc acgcccagag
     1561 cagcaaaatc ttgctcaggt ttactccctg cgctggcacc tagccagatc tccagtaaca
     1621 cgccaatcca gcccttatcg cgttttaaat tctctggcgt aaccagcccg gcaagtgccg
     1681 ccagttctcc caatgtataa ccagaaagtt gctgtgcttg cgctaacaac tgttcttcag
     1741 tttcgggagg agagagcagt gggcgaggtt gggacatgtc atgatgcctt ggttaaaaaa
     1801 caaacagcaa ttatgtgccg aatattcgca gtgtaacttt ttccacatgt gttgttcaat
     1861 tctctgattt atattgcttt ttaggctgcc ggtaatcgag ttgcgcaaca ctgcaatagg
     1921 ttttccaaat ctggtcactg aaaataaaca ggatcttaca ccatgttatc cacagaaagc
     1981 tgggataact gtgaaaaacc tccactactg ttttcattta cagccttgac gtgcgacgaa
     2041 actccagttt ttagtggttc agtgcttaac ttagtggcac attctgtgga taaatacggc
     2101 attggttgat ctttcgccaa cccgtaaatg agaggtgtga cctgggtcac gaattactct
     2161 ttttcattac tcaaattaca tgtaatgcat tgactaataa tgaatatttt aaaatgtcta
     2221 tatcgggctt attcagaatg ctccgggttt tcccgtagta attccgtagt tcttcacaac
     2281 tctatccaca gaaaaggtga ataaaaacgg ctatccaccc cttcctctgt ttataactct
     2341 gcacataact gtgagttatt caattgttat tagtgtgtaa tgccctgcag agagtggttt
     2401 accgtcttcc tggagtatga aacaatcatt cgtatataaa gctttatttt gaggtagtcc
     2461 ggtgattgat gacgatggct accgcccaaa cgtaggtatc gtgatttgta atcgccaggg
     2521 gcaggtaatg tgggcccggc gatttggtca gcactcctgg caatttccgc aaggcggaat
     2581 caaccccgga gaatccgcag agcaggcgat gtaccgtgaa ttgtttgaag aagtaggatt
     2641 aagccgcaaa gacgttcgaa tccttgcttc aacgcgtaac tggttgcgct acaaattacc
     2701 gaaacgtttg gtgcgttggg acacgaagcc ggtttgtatc ggccaaaaac aaaaatggtt
     2761 tctcttgcag ctggtaagcg gcgatgcaga aatcaatatg caaaccagca gtacaccaga
     2821 gtttgacggc tggcgatggg taagttactg gtatccggtc agacaggtgg tgtcatttaa
     2881 acgtgatgtc taccgtaggg taatgaaaga gttcgcgagt gtggtgatgt cactgcagga
     2941 aaatacgcca aaaccacaaa acgcatctgc ttatcgacgt aaaagaggtt aagtcacgcc
     3001 aattatgctc actcgcctgc gcgaaatagt cgaaaaggta gccagcgcac cacgcctgaa
     3061 tgaggcgtta aatattctgg ttaccgacat ctgtcttgcg atggataccg aggtctgttc
     3121 ggtctacctg gccgatcatg atcgacgttg ttactacctg atggcgaccc gggggctgaa
     3181 aaaaccacgc ggtcgcactg taacgctcgc gtttgatgaa gggatcgtcg gcctggttgg
     3241 caggctggcg gaaccgataa accttgcaga tgcgcaaaag caccccagct tcaaatacat
     3301 cccctccgta aaagaagaac gtttccgcgc gtttttaggc gtaccaatta ttcaacgtcg
     3361 ccagttgctt ggtgtactgg tggtacagca acgagagttg cgccagtatg acgaaagtga
     3421 agaatccttc ctggtgacgc ttgccaccca gatggcagct attctttctc agtcgcagtt
     3481 gactgccctg tttgggcaat atcgccagac gcgaatccgc gcattaccgg cagcacctgg
     3541 tgtggcgatt gccgaaggct ggcaggatgc cacgttacct ttaatggaac aggtgtatca
     3601 ggcatcaacg ctggatccgg ctctggaacg cgaacgactg accggggcgc tggaagaagc
     3661 ggcaaacgag tttcgccgct acagcaaacg ctttgccgcc ggagcacaaa aagaaacggc
     3721 ggctattttc gatctttact cgcacctgct ttcggacacc cggctgcgtc gcgaattgtt
     3781 tgccgaggtt gataaaggct cggtggcaga gtgggcggta aaaacggtca ttgaaaaatt
     3841 tgccgaacag tttgccgcgc taagcgataa ctatctcaaa gagcgggctg gcgatttacg
     3901 tgcgctgggt cagcgattgc tgtttcatct tgatgacgct aatcaagggc cgaacgcctg
     3961 gccggaacgt ttcattctgg tggcagatga actgtcagcg acaactcttg ctgagctgcc
     4021 ccaggatcgc ttagtcggtg ttgtcgtgcg agatggcgca gccaactccc atgctgcgat
     4081 catggtacgt gcgctgggga tccctaccgt gatgggcgcg gatattcagc cttcggtgct
     4141 gcatcgtagg acgctgatcg ttgatggcta tcgcggtgaa ttgctggtcg atccggagcc
     4201 ggtactgctg caagaatatc agcggctaat tagtgaagag attgagctta gccgtctggc
     4261 ggaagatgac gtcaatttac ccgcgcagtt aaaaagcggt gagcgtataa aagtcatgct
     4321 caatgctggt ttaagcccgg aacatgaaga aaaactgggc agccgtattg atggcatcgg
     4381 tctttatcgc actgaaatcc cattcatgct gcaaagtggt ttcccgtcgg aagaagaaca
     4441 ggtggcgcag tatcagggga tgctgcaaat gttcaatgat aaacccgtca ccttgcgtac
     4501 gctggatgtc ggagcagata agcagctgcc ttacatgccg atcagcgaag agaatccatg
     4561 cctgggttgg cgtggtattc gcattacgct cgatcagccg gagatcttct tgatccaggt
     4621 gcgggcgatg ctgcgtgcta atgccgctac gggcaacctg aatattctgt tgccgatggt
     4681 caccagcctc gatgaagttg acgaagcacg ccgcctgatt gaacgtgccg gacgtgaagt
     4741 cgaggagatg atcggttacg aaattcccaa accacgtatc ggcatcatgc tggaagtgcc
     4801 gtcaatggta tttatgctgc cgcatctggc aaagcgggtc gatttcatct ctgttggcac
     4861 caacgatctg actcaataca ttctggccgt tgatcgcaac aatacccggg tggctaacat
     4921 ttacgacagt cttcatcctg caatgttacg agctctggcg atgatcgccc gggaagcgga
     4981 aatacatggt atcgatctcc gtttgtgcgg tgaaatggcg ggcgatccca tgtgtgtggc
     5041 aatcctcatt gggcttgggt atcgccatct gtctatgaac ggacgttctg tagcgcgggt
     5101 aaaatacctg ctgcggcgca ttgattttgc cgaagcagaa aatcttgcgc agcgtagtct
     5161 ggaagcgcaa ctggcgaccg aagttcgtca tcaggttgca gcctttatgg agcgtcgcgg
     5221 catgggcggg ctgattcgcg gagggttata gcgcggatca tatacatatc ttttaacggt
     5281 atccggcaac cagccaggtc cccttgtgct attattcgca cctttggagc gcctgaaacc
     5341 tgcggcgcgc atttcaatcg ctgttctctt tcagcgaaat aacaagaact tgtggtgaca
     5401 gatgaccagt agctatctgc attttccgga gtttgatccg gtcattttct caataggacc
     5461 cgtggcgctt cactggtacg gcctgatgta tctggtgggt ttcatttttg caatgtggct
     5521 ggcaacacgt cgggcgaatc gtccgggcag tggctggacc aaaaatgaag ttgaaaactt
     5581 actctatgcg ggcttcctcg gcgtcttcct cgggggacgt attggttatg ttctgttcta
     5641 caatttcccg cagtttatgg ccgatccgct gtatctgttc cgtgtctggg acggcggcat
     5701 gtctttccac ggcggcctga ttggcgttat cgtggtgatg attatcttcg cccgccgtac
     5761 taaacgttcc ttcttccagg tctctgattt tatcgcacca ctcattccgt ttggtcttgg
     5821 tgccgggcgt ctgggcaact ttattaacag tgaattgtgg ggccgcgttg acccgaactt
     5881 cccgtttgcc atgctgttcc caggctcccg tacagaagat attttgctgc tgcaaaccaa
     5941 cccgcagtgg caatccattt tcgacactta cggtgtgctg ccgcgccacc catcacagct
     6001 ttacgaactg ctgctggaag gtgtggtgct gtttattatc ctcaacctgt atattcgtaa
     6061 accgcgccca atgggagctg tctcaggctt gttcctgatt ggctacggcg cgtttcgcat
     6121 cattgttgag tttttccgcc agcccgacgc gcagtttacc ggtgcctggg tgcagtacat
     6181 cagcatgggg caaattcttt ccatcccgat gattgtcgcg ggtgtgatca tgatggtctg
     6241 ggcatatcgt cgcagcccac agcaacacgt ttcctgagga accatgaaac agtatttaga
     6301 actgatgcaa aaagtgctcg acgaaggcac acagaaaaac gaccgtaccg gaaccggaac
     6361 gctttccatt tttggtcatc agatgcgttt taacctgcag gatggattcc cgctggtgac
     6421 aaccaaacgt tgccacctgc gttccatcat ccacgaactg ctgtggttcc tgcagggcga
     6481 tactaacatt gcttatctac acgaaaacaa tgtcaccatc tgggacgaat gggccgatga
     6541 aaatggcgac ctcgggccag tgtatggtaa acagtggcgc gcctggccaa cgccagatgg
     6601 tcgtcatatt gaccagatta ctacggtact gaaccagctg aaaaacgacc cggattcgcg
     6661 ccgcattatt gtttcagcgt ggaacgtagg cgaactggat aaaatggcgc tggcaccgtg
     6721 ccatgcattc ttccagttct atgtggcaga cggcaaactc tcttgccagc tttatcagcg
     6781 ctcctgtgac gtcttcctcg gcctgccgtt caacattgcc agctacgcgt tactggtgca
     6841 tatgatggcg cagcagtgcg atctggaagt gggtgatttt gtctggaccg gtggcgatac
     6901 gcacctgtac agcaaccata tggatcaaac tcatctgcaa ttaagccgcg aaccgcgtcc
     6961 gctgccgaag ttgattatca aacgtaaacc cgaatccatc ttcgactacc gtttcgaaga
     7021 ctttgagatt gaaggctacg atccacatcc gggcattaaa gcgccggtgg ctatctaatt
     7081 acgaaacata ctgccagagc cgacgccagt gtgcgtcggt ttttttaccc tccgttaaat
     7141 tcttcgagac gccttcccga aattttgcaa cgtcctgcaa cggcgtaaat agtccggaag
     7201 atgcgccgaa gaaatagaaa ctggccctcg tctttttttc tcctcgctcc atactgccgg
     7261 catgaaaaca caacgtggtt atacgctgat tgaaacgctg gtcgcgatgc tgattttggt
     7321 catgctaagc gcaagtggac tctatggctg gcaatactgg cagcagtcgc aacggctttg
     7381 gcaaaccgcc agctaggcgc gggactattt gctctattta cgtgaagatg ccaactggca
     7441 taaccgcgac cacagtatca gcgttatcag ggaggggacg ttatggtgcc ttgtgagttc
     7501 cgctgctggg gccaatacct gtcatggcag ttcaccattg gtctttgtgc cacgctggcc
     7561 cgaagtcgaa atgagcgacc tgacaccttc gcttgctttc tttggcctgc gcaataccgc
     7621 atgggccggg catattcgct tcaaaaactc aacgggcgag tggtggctgg tggtttcgcc
     7681 gtggggaaga ctccggcttt atcagcaagg agaaacagaa ggatgcctgt aaaagagcaa
     7741 ggtttttctc tgctggaagt gttgattgct atggcgatca gtagcgtatt gttgctgggg
     7801 gctgcacgct ttctgcctgc gttacagcgt gaaagtttaa cgagcactcg taagctggca
     7861 ctggaagatg aaatctggct gcgggtattt accgtcgcga agcatctcca gagggcgggt
     7921 tattgtcatg gcagctgtac cggcgaaggg ctggaaattg tcggacaggg tgactgtatc
     7981 attgtgcagt gggatgcgaa cagtaacggt atctgggatc gcgaaccggt aaaagagtcc
     8041 gaccagattg gatttcgtct gaaggagcat gtgctggaaa cgctacgcgg tgcgacatcc
     8101 tgtgaaggta agggctggga taaagtcact aatccggatg ccatcattat cgacactttt
     8161 caggtcgtac gtcaggatgt cagcggcttc tcgccggtgt tgacggttaa tatgcgtgct
     8221 gccagtaagt ctgaaccgca aaccgtggtg gatgccagct atagcgtgac aggattcaac
     8281 ctgtgaaccg cgaaaaaggt gtttcgtcac aggctctggt cctgatgctg ctggttttgg
     8341 gtagcttgct attgcaagga atgagtcagc aggatcgcag ttttgcctct cgcgtgagca
     8401 tggaaagtca gtcattgcgc cgccaggcca tcgttcagtc ggcgctggcg tggggaaaaa
     8461 tgcactcctg gcagacgcag ccagcagttc agtgctcgca gtacgctgga accgatgccc
     8521 aggtttgttt gcgtttactg gcagataatg aagccttatt gattgccggt tatgaaggcg
     8581 tttcgttgtg gcgaacaggc gaagtcattg atggaaaaat tgttttttcg ccacgcggct
     8641 ggagcgattt ttgtccgctg aaagaggggg cattatgtca gcttccctga agaatcaaca
     8701 aggctttagc ctgccggagg taatggttgc gatggtgctg atggtgatga ttgtcactgc
     8761 gttatcgggt atccagcgaa cattaatgaa cagtctcgcc agcagaaacc agtaccaaca
     8821 gctctggcgg catggttggc agcaaacgca actgcgcgcg atttcgccac ttgccaactg
     8881 gcaggtcaac cgaatgcaga catcgcaggc gggatgtgtc agcatcagcg ttacgctagt
     8941 ttcacccggg ggcagagaag gcgagatgac ccgcctgcat tgcccgaatc gtcagtagtc
     9001 aggagccgct atgttaaggg tctaccattc caatcgtctg gacgtgctgg aagcgttgat
     9061 ggagtttatt gtcgaacgcg aacggctgga cgatcctttc gaaccagaga tgattctggt
     9121 gcaaagtacc ggtatggcgc agtggctgca aatgaccctg tcgcaaaagt ttggtattgc
     9181 ggcaaacatt gattttccgc tgccagcgag ctttatctgg gatatgttcg tccgggtgtt
     9241 accggaaatc cccaaagaga gcgcctttaa caaacagagc atgagctgga aactgatgac
     9301 tctgctgccg caattgctgg agcgcgaaga ctttaccctg ttgcggcatt atctgactga
     9361 cgatagcgac aagcgaaaac tgttccagct ttcctcaaaa gcggcggacc tgtttgacca
     9421 gtatctggtc tatcgtccgg actggctggc acagtgggaa acaggacatt tggttgaagg
     9481 gctgggagaa gcacaggcct ggcaagcccc gttgtggaag gcgctggtgg aatataccca
     9541 tgaactcgga caaccgcgct ggcaccgcgc gaatctctat cagcgcttta tcgaaacgct
     9601 ggagtccgcg acgacctgcc cgccggggtt accttcgcgc gtctttatat gcggtatttc
     9661 cgcgttaccg cctgtttatc tccaggcgct ccaggcgctg ggtaaacata ttgaaatcca
     9721 tctcctgttt accaacccct gccgttatta ctggggcgat attaaagatc ctgcttatct
     9781 ggcgaaactg ctgactcgtc agcgtcgaca cagttttgaa gatcgcgaat tgccgctatt
     9841 tcgcgatagc gaaaatgccg gacagctctt taacagcgat ggtgaacagg atgtcggcaa
     9901 cccgctgctg gcctcatggg ggaagcttgg gcgcgactac atttatctcc tttctgacct
     9961 ggagagcagc caggagctgg acgcttttgt cgatgtgacg ccagataacc tgctgcataa
    10021 cattcagtct gacattctgg aactggaaaa ccgcgccgtt gcgggtgtga acatcgaaga
    10081 gttttcccgt agcgataaca aacgcccgct tgatccactg gatagcagta tcacattcca
    10141 cgtttgccat agcccgcagc gtgaagttga agttttacac gatcgcttgc tggcgatgct
    10201 ggaggaagcc ccgacactta ctccgcgcga catcatcgtg atggtggctg atatcgacag
    10261 ctacagtccg tttattcagg cagtgtttgg tagcgcacct gcggatcgtt acctgcctta
    10321 cgccatttcc gaccgtcgtg cgcggcagtc acatccggta ctggaagcgt ttatcagcct
    10381 gttatcactg cctgacagtc gctttgtgtc agaggatgtg ctggcgctgc tggatgtgcc
    10441 ggtgctggcg gcgcggtttg acatcaccga agaagggctg cgttatttac gccagtgggt
    10501 caacgaatcc ggcattcgtt gggggataga tgacgacaac gttcgcgagc tggaactccc
    10561 cgccaccgga caacacacct ggcgatttgg cctgacgcgt atgttgctgg gctacgcgat
    10621 ggagagcgcg cagggcgagt ggcaatcggt tctaccttat gatgaatcga gcggcttaat
    10681 tgcagaactg gtggggcatc tggcttcact gctaatgcag ctaaacatct ggcgtcgcgg
    10741 gctggcacag gagcgtccgc tagaagagtg gttgccggtt tgtcgcgata tgctcaacgc
    10801 cttcttcctg ccggatgcgg aaaccgaagc ggcgatgacg ctgatcgaac aacaatggca
    10861 ggcgattatc gccgaaggtt taggtgcgca gtatggcgac gcggtgccgc tgtcactatt
    10921 gcgtgatgaa ctggcacagc gtctggatca agaacgtatc agccagcgtt ttctcgccgg
    10981 accggttaat atttgtactc tgatgccaat gcgttcaatt ccgttcaaag tggtttgcct
    11041 gctgggaatg aacgacggcg tttatccacg tcagcttgcg ccattgggct ttgacctgat
    11101 gagccagaaa ccgaagcgtg gcgaccgtag ccgtcgcgat gacgaccgct acctgttcct
    11161 ggaagcgtta atttccgcgc agcaaaaact ctatatcagc tatatcgggc gttccattca
    11221 ggataacagt gaacgtttcc cgtcggtact ggtgcaggaa ctgatcgact acatcgggca
    11281 aagtcattat ctaccgggcg atgaagcgct caactgtgat gaaagcgagg caagggtaaa
    11341 agcgcatctt acttgcctcc atacccggat gccgtttgat ccgcaaaact accagccagg
    11401 cgaacgacaa agctatgctc gtgaatggct acctgcggcc agccaggctg gtaaagcaca
    11461 ttctgaattt gttcagccgc tgccgtttac cttaccggaa accgtgccgc tggaaacgct
    11521 acaacgattc tgggcacatc cggtgcgggc gttcttccag atgcgtttgc aggtgaactt
    11581 ccgtactgaa gacagcgaaa tcccagacac cgagccattt attctggaag gacttagccg
    11641 ttatcaaatc aatcagcagt tattgaatgc actggttgag caggatgatg ccgaacgctt
    11701 gttccgccgc ttccgggcgg caggggattt accgtatggc gcttttggtg aaattttctg
    11761 ggaaacacag tgccaggaga tgcagcagct tgccgacaga gtcattgcct gtcgccagcc
    11821 ggggcagagt atggaaattg atctcgcctg caacggtgtg cagataactg gctggttgcc
    11881 gcaggtgcag ccggatggcc tgttgcgctg gcgtccctct ttattaagtg tggcgcaggg
    11941 aatgcaactt tggctggaac accttgtcta ctgtgccagc ggtggtaatg gcgaaagtcg
    12001 cctttttcta cgcaaagacg gtgagtggcg ttttccgccg cttgcagccg aacaggcttt
    12061 gcattacctc tcacaactga ttgaggggta tcgtgaagga atgtccgcgc cattgctggt
    12121 gttacctgaa agtggcggcg cgtggctaaa aacctgttat gacgcgcaaa acgatgccat
    12181 gctggatgac gattctacgt tgcaaaaagc ccgtacgaaa ttccttcagg cttacgaagg
    12241 caacatgatg gtgcgtggcg aaggtgatga tatctggtat caacggctct ggcggcaatt
    12301 aacaccagag acaatggagg ccatcgttga acagtcgcaa cgtttcctgt taccgctgtt
    12361 tcgctttaat cagtcatgag tgttgtataa aaattgcgca atctatccgc ttactttatg
    12421 atgcgcacca gtcacggact gatggttata taaacatagg ctgactcgtg cagcacaaga
    12481 ttaaattctg gcagatgatt tgcgttaacc tgttgaatct gtccagaaaa taaagttgat
    12541 tatgaggtcc gtgaatgccc cgcagcacct ggttcaaagc attattgttg tttgttgccc
    12601 tctgggcacc attaagtcag gcagaaacgg gatggcagcc gattcaggaa accatccgta
    12661 aaagtgataa agataaccgc cagtatcagg ctatacgtct ggataacggt atggtggtct
    12721 tgctggtttc tgatccgcag gcagttaaat cgctctcggc gctggtagtg cccgttgggt
    12781 cgctggaaga tcccgaggcg taccaggggc tggcacatta ccttgaacat atgagtctga
    12841 tggggtcgaa aaagtacccg caggctgaca gtctggccga atatctcaaa atgcacggcg
    12901 gtagtcacaa tgccagcact gcgccgtatc gcacggcttt ctatctggaa gttgagaacg
    12961 acgccttgcc cggtgcggta gaccgcatgg ccgatgctat tgcagaaccc ttgctcgaca
    13021 agaaatacgc cgaacgtgag cgtaatgcag tgaacgctga attaaccatg gcgcgtacgc
    13081 gtgacgggat gcgcatggca caggtcagcg cagaaaccat taacccggca caccccggtt
    13141 caaagttttc tggtggtaac ctcgaaactt taagcgacaa acctggtaat ccggtgcagc
    13201 aggcgctgaa agatttccac gagaagtact attccgccaa tttgatgaag gcggttattt
    13261 acagtaataa accgctgccg gagttggcaa aaatggcggc ggacaccttt ggtcgcgtgc
    13321 cgaacaaaga gagcaaaaaa acggaaatca ccgtgccggt agtcaccgac gcgcaaaagg
    13381 gcattatcat tcattacgtc cctgcgctgc cgcgtaaagt gttgcgcgtt gagtttcgca
    13441 tcgataacaa ctcagcgaag ttccgtagta aaaccgatga attgattacc tatctgattg
    13501 gcaatcgcag cccaggtaca ctttctgact ggctgcaaaa gcagggatta gttgagggca
    13561 ttagcgccaa ctccgatcct atcgtcaacg gcaacagcgg cgtattagcg atctctgcgt
    13621 ctttaactga taaaggcctg gctaatcgcg atcaggttgt ggcggcaatt tttagctatc
    13681 tcaatctgtt acgtgaaaaa ggcattgata aacaatactt cgatgaactg gcgaatgtgc
    13741 tggatatcga cttccgttat ccgtcgatca cccgtgatat ggattacgtc gaatggctgg
    13801 cagataccat gattcgcgtt cctgttgagc atacgctgga tgcagtcaat attgccgatc
    13861 ggtacgatgc taaagcagta aaggaacgtc tggcgatgat gacgccgcag aatgcgcgta
    13921 tctggtatat cagcccgaaa gagccgcaca acaaaacggc ttactttgtc gatgcgccgt
    13981 atcaggtcga taaaatcagc gcacaaactt tcgccgactg gcagaaaaaa gccgccgaca
    14041 ttgcgctctc tttgccagag cttaaccctt atattcctga tgatttctcg ttgattaagt
    14101 cagagaagaa atacgaccat ccagagctga ttgttgatga gtcgaatctg cgcgtggtgt
    14161 atgcgccaag ccgttatttt gccagcgagc ccaaagctga tgtcagcctg attttgcgta
    14221 atccgaaagc catggacagc gcccgcaatc aggtgatgtt tgcgctcaat gattatctcg
    14281 cagggctggc gcttgatcag ttaagcaacc aggcgtcggt tggtggcata agtttttcca
    14341 ccaacgctaa caacggcctt atggttaatg ctaatggtta cacccagcgt ctgccgcagc
    14401 tgttccaggc attgctcgag gggtacttta gctataccgc tacggaagag cagcttgagc
    14461 aggcgaagtc ctggtataac cagatgatgg attccgcaga aaagggtaaa gcgtttgagc
    14521 aggcgattat gcccgcgcag atgctctcgc aagtgccgta cttctcgcga gatgaacggc
    14581 gtaaaatttt gccctccatt acgttgaaag aggtgctggc ctatcgcgac gccttaaaat
    14641 caggggctcg accagagttt atggttatcg gcaacatgac cgaggcccag gcaacaacgc
    14701 tggcacgcga tgtgcaaaaa cagttgggcg ctgatggttc agagtggtgt cgaaacaaag
    14761 atgtagtggt cgataaaaaa caatccgtca tctttgaaaa agccggtaac agcaccgact
    14821 ccgcactggc agcggtattt gtaccgactg gctacgatga atacaccagc tcagcctata
    14881 gctctctgtt ggggcagatc gtacagccgt ggttctacaa tcagttgcgt accgaagaac
    14941 aattgggcta tgccgtgttt gcgtttccaa tgagcgtggg gcgtcagtgg ggcatgggct
    15001 tccttttgca aagcaatgat aaacagcctt cattcttgtg ggagcgttac aaggcgtttt
    15061 tcccaaccgc agaggcaaaa ttgcgagcga tgaagccaga tgagtttgcg caaatccagc
    15121 aggcggtaat tacccagatg ctgcaggcac cgcaaacgct cggcgaagaa gcatcgaagt
    15181 taagtaaaga tttcgatcgc ggcaatatgc gcttcgattc gcgtgataaa atcgtggccc
    15241 agataaaact gctgacgccg caaaaacttg ctgatttctt ccatcaggcg gtggtcgagc
    15301 cgcaaggcat ggctattctg tcgcagattt ccggcagcca gaacgggaaa gccgaatatg
    15361 tgcatcctga aggctggaaa gtgtgggaga acgtcagcgc gttgcagcaa acaatgcccc
    15421 tgatgagtga aaagaatgag tgatgtcgcc gagacactag atcctttgcg cttgccctta
    15481 cagggcgagc gcctgattga agcctctgcc ggcacaggca aaacctttac gattgcggcg
    15541 ctctatttgc gcctgttact tggactaggc ggttccgccg cctttccccg cccgctgatc
    15601 gttgaagaac tgctggtggt cacctttacc gaggctgcca cggcagaatt gcgcggtcgt
    15661 atccgtagca atatccacga gttgcgcatc gcctgtctgc gtgaaaccac cgacaatcca
    15721 ctgtacgaac gcctgctgga agagatcgac gataaagcgc aagccgcgca gtggttgttg
    15781 ttagccgaac ggcagatgga tgaagcggca gtctttacta ttcacggctt ttgccagcgc
    15841 atgctcaacc tgaatgcctt tgaatccggc atgctgtttg agcagcagct gattgaagat
    15901 gagtctctgc tacgctacca ggcctgcgcc gatttctggc gtcgccactg ctacccgctg
    15961 ccgcgtgaaa tagcccaggt cgtctttgaa acctggaaag ggccgcaggc gttgctgcgc
    16021 gatattaatc gttatctgca aggcgaagcg ccggttatca aagcaccgcc gcccgatgat
    16081 gaaacgctgg cttcccgcca cgcgcaaatt gtggcgcgta ttgatacggt aaaacagcag
    16141 tggcgcgacg cagtgggtga actggatgcg ctgatcgaat cttctggtat tgatcgacgc
    16201 aagtttaacc gtagcaatca ggctaaatgg atcgaaaaga tcagcgcttg ggcagaagaa
    16261 gagacaaaca gttatcagtt gccggagtcg ctggaaaaat tctcccagcg tttcttagaa
    16321 gatcgcacga aggccggggg ggaaaccccg cgacatccac tgtttgaggt gatcgatcaa
    16381 ctgcttgcag aaccattgtc gatccgcgat ctggtgatca cccgagcatt ggctgagatc
    16441 cgcgaaacag tagcgcgtga aaaacgccgc cgtggcgaat tgggttttga tgacatgtta
    16501 agtcggctcg attccgcgct gcgtagcgaa agcggcgagg tgttggcagc ggcgatccgt
    16561 acgcgattcc cggtggcaat gatcgatgaa tttcaggata ccgaccccca gcagtaccga
    16621 atttttcgcc gtatctggca ccatcagccg gaaaccgcat tgttgctgat tggcgacccg
    16681 aagcaggcca tatatgcatt ccggggtgcg gatatcttca cttatatgaa ggcgcgtagt
    16741 gaagttcacg cccactacac tttagacacc aactggcgct ccgcacctgg aatggtgaac
    16801 agtgtgaaca agcttttcag ccagactgat gacgcgttca tgtttcgcga aataccgttt
    16861 attccagtga aatcagccgg gaaaaatcag gcgttacgtt ttgtatttaa aggtgaaacg
    16921 cagcctgcga tgaaaatgtg gctgatggaa ggcgaaagct gcggcgttgg cgactatcaa
    16981 agtaccatgg cgcaggtatg tgctgcgcaa atccgcgact ggctacaagc cggacagcgg
    17041 ggcgaagcgt tgctgatgaa cgacgacgac gcgcgtccgg tgcgtgcttc ggacatcagc
    17101 gtgctggtgc gcagccgcca ggaggccgcc caggtgcgcg atgccttaac gttgctggaa
    17161 atcccttccg tttacctttc gaaccgcgac agtgtttttg aaactctgga agcgcaggaa
    17221 atgctttggt tgttgcaggc ggtgatgacg cccgaacgtg agaacaccct gcgcagtgcg
    17281 ctggcaacgt caatgatggg gctgaatgcg ctggatattg aaacgctgaa taatgacgaa
    17341 catgcgtggg atgcggtagt cgaagagttc gatggttatc ggcaaatctg gcgcaaacgt
    17401 ggcgttatgc cgatgctgcg ggcgctgatg tcggcgcgta acattgcaga aaacttgctg
    17461 gcaacggcag gcggtgagcg gcgtcttacc gatatcttgc atatcagcga actgcttcaa
    17521 gaagccggaa cgcagctgga aagtgaacat gcgctggtac gctggttatc gcaacatatc
    17581 ctcgagccag acagtaatgc ctccagccaa caaatgcgcc tcgaaagtga taaacatctg
    17641 gtgcagattg tcacgatcca taaatcgaaa gggctggaat atccgctggt ctggttgcca
    17701 tttattacca atttccgcgt ccaggatcag gcgttttatc acgatcgcca ctcgtttgag
    17761 gcagttttgg atcttaatgc tgcgccagaa agcgtcgacc tcgcggaggt cgaacgtctg
    17821 gcggaagatc tgcgtttgct ttacgtggcg ctgacgcgtt cggtttggca ttgcagtctc
    17881 ggcgttgcac cgctggtgcg ccgtcgtggc gataaaaaag gtgacaccga cgtccaccaa
    17941 agtgcgctcg ggcgtttgct gcaaaaaggg gaaccgcaag atgcggcagg gcttcgcacc
    18001 tgtattgaag cgttatgcga tgatgatatt gcctggcaaa cggcacaaac tggtgataac
    18061 cagccctggc aggttaatga tgctttaact gcagaactga atgcgaggac actacaacga
    18121 ttgcccggcg ataactggcg tgtcaccagc tactccggct tgcagcagcg tggtcacggt
    18181 atcgctcagg atctgatgcc acggctggat gttgatgccg caggcgtggt cagcgtcgtt
    18241 gaagaaccga cgttaacacc gcatcagttc ccgcgcggtg cgtcaccggg gacattcttg
    18301 cacagtttgt ttgaagacct cgattttacc cagccggttg acccgaactg ggtacaggaa
    18361 aaactggaac tcggcggctt tgaatcgcag tgggaaccgg tgttgaccga gtggatcacg
    18421 gctgtcctcc aggcacctct caatgaaacg ggtgttagcc tgagtcagct ttccgaccgc
    18481 gataaacagg tggagatgga gttttacctg ccgattagcg aaccgctcat cgccagccag
    18541 cttgatgcac taattcgcca gtttgacccg ctatccgctg gctgcccgcc gctggagttc
    18601 atgcaggtac gcggcatgtt aaaaggcttt atcgacctgg tattccgcca cgaagggcgt
    18661 tattacctgc tcgactataa atccaactgg ctgggtgaag acagttcggc ttacacccaa
    18721 caggctatgg cagcagcgat gcaggcacac cgctatgatc tgcaatatca gctttatacc
    18781 ctggcactgc atcgttatct gcgccatcgc attgctgatt acgactatga tcttcacttt
    18841 ggcggcgtta tctatctgtt cctgcgtggc gttgataaag aacatccgca acaagggatt
    18901 tacacaaccc gacccaacgc cgggttgatt gccctgatgg atgagatgtt tgccggtatg
    18961 accctggagg aggcgtaatg aaattgcaaa agcaattact ggaagctgtg gagcacaaac
    19021 agctacgccc gctggatgtg caatttgccc tgaccgtggc gggagatgaa catcctgctg
    19081 tcaccctcgc ggcggcactg ttaagtcatg atgccggaga gggacacgtc tgtttgccgc
    19141 tttcacgact ggaaaataac gaggcgtcgc atccgctgtt ggcgacctgt gtcagtgaaa
    19201 tcggtgagct acaaaattgg gaagaatgct tgctggcttc tcaagcggtc agcaggggag
    19261 atgaacccac gccgatgatc ctctgtggcg atcgtcttta tttgaatcgc atgtggtgta
    19321 acgagcggac ggtagcgcgc ttcttcaatg aggtgaatca taccattgag gttgatgaag
    19381 ctctactggc gcaaaccctg gacaaacttt ttccagtaag cgatgaaatt aactggcaaa
    19441 aagttgcagc ggcagtggcg ctgacgcggc ggatctcggt gatttccggc ggcccaggca
    19501 ccggtaaaac gaccaccgtg gcgaagttgc tggcagcgtt aattcaaatg gccgacggcg
    19561 aacgctgccg tatccgtctg gctgcaccaa cgggtaaagc tgccgcgcgc ttaaccgaat
    19621 ctctcggcaa ggctttgcga cagttaccgc tgaccgatga acaaaagaaa cgcattccgg
    19681 aagatgccag cactttgcac cgattgctgg gcgcgcagcc gggtagccag cgtttacgtc
    19741 atcatgccgg taacccgctg catcttgatg tgctggtggt agatgaagcg tcaatgatcg
    19801 atctgcctat gatgtcgaga ctgatcgacg ccttgccaga tcatgcgcga gtgatctttc
    19861 tcggcgatcg tgatcaactg gcctcggttg aggctggggc tgtgctgggc gatatctgcg
    19921 cttatgccaa cgcgggcttt accgccgagc gtgccaggca gctaagccgc ctgacgggga
    19981 ctcacgttcc ggcaggaacc ggcacagaag cggcatcttt gcgcgacagt ctctgcctgc
    20041 tgcaaaaaag ctatcgtttc ggcagcgatt ctggcattgg tcagttagct gcggcgatca
    20101 accgtggtga taaaacggca gtgaaaaccg tttttcagca ggattttact gatatcgaaa
    20161 aacgactttt acagagcggc gaagattata ttgcgatgct tgaggaagct cttgcgggtt
    20221 acggacgtta tctggatctg ctgcaagcgc gtgccgagcc ggatttaatc attcaggcgt
    20281 tcaatgagta ccagcttttg tgcgccctgc gggaagggcc gtttggcgtg gctggactga
    20341 atgagcgaat tgagcagttt atgcaacaga agcgcaaaat tcatcgtcat ccgcactctc
    20401 gttggtacga aggccgaccg gtgatgattg cccgtaatga cagcgcgctt gggttgttta
    20461 atggcgatat cggtattgcg ctggatcgcg ggcaggggac gcgcgtctgg tttgcgatgc
    20521 cggacggcaa tattaagtct gtgcaaccga gtcgcttgcc agagcacgaa actacgtggg
    20581 cgatgacggt acataaatcg cagggatcgg agttcgacca tgcggcgttg attttaccga
    20641 gccaacgcac gccggtagta acgcgagagc tggtatacac cgcggtgacc cgcgcgcgtc
    20701 gtcgtctgtc gctgtatgcc gatgagcgta tattaagtgc ggcaatcgcc actcgtactg
    20761 agcggcgcag tggtctggcg gcgttgttta gttcgcggga ataaacgtaa ttgccggatg
    20821 cgacgcacga gtgttacgcc tgccgcatcc ggtgattttc atcgcttacc ctaaatccgc
    20881 catcaacact ttggatttac gctggtagtt gtacaactgc tttttgctct cgggcagtaa
    20941 atcaatatcc accggggtaa atccacgttc ctggaaccag tgaatactgc gcgtggtcag
    21001 cacaaacaat ttgcttaagc cgctctgctt cgcctgagcg gcaatgcgtt ccagcagaac
    21061 ctcgccccgt gatgaactgc ggtaatccgg gtgaactgcc acacaggcca tttccccaat
    21121 cttctcttcc gggaacggat agagcgcggc gcaggcaata gtcgtgttat cgcgctgaat
    21181 aatggtgaat ttgtcgattt ccatctccag ctgctcgcga gaacggcgca ccagaatacc
    21241 ttgctgctcc agcgggcgaa tcaactccag aataccgcca atatcgttga ttgttgcgcg
    21301 acgaatctgc tcggcgcttt ccatcacaat ctgcgtaccg ataccgtcgc gtgagaacaa
    21361 ctcttgcaac agcgcgccat cttcctgata actgattaaa tgacagcggc gcacgccgct
    21421 gcggcaggct ttcactgcgc cacgcaaaaa gcgcaccgta ccggagttgt aatcgccttt
    21481 ctcttcctgg gcttctaccc gtgcttgcgc ttcgttaggg aaaagttcgg agacaatatc
    21541 accgtcgtca ttagtaacgc cctgggaaga gcaaaaacca atcatctttt cagctttcag
    21601 tttgatggct agttgagtgg caatctcttc agaggtcaga ttaaagctct cgccagtgac
    21661 tgaaacagcg accggcccca ttagcactat tgcaccgctt tccagttgac gatggatcgc
    21721 gtcttcatca atccgccgga tacgcccgct atggcagtaa tccacgccgt catcgacgcc
    21781 cagcggctgg gcaataataa aattgccact aacgacgttg atatgcgcgc cctgcagcgg
    21841 cgtgttattg agactcattg acaggcgagc agtaatatcc agttgcaatg ttcccgcagc
    21901 ctgcttcacc agttccagtg ttttggcgtc ggtcacacgt atattcttgt gatacagcgg
    21961 ttcgtggtga tgcgcagcca gatttgcgtc gatctgcgga cgtgcgccat agaccaccac
    22021 cagacggatg ccgaggctgt gcaacaaccc gatatcatta acgatactgg agaaattctc
    22081 atgctcaatg gcttcaccgc cgagcatgat gacaaacgtt tttccccggt gggtattgat
    22141 atagggaacc gaatggcgga atccctcgac cagctcggtt ttacgttcct ttaccacggc
    22201 acaccccttt gcatgattat ttgaaattat tgtattttta ttctgttttt tcgcagggtg
    22261 caagtgtaaa ttttatgcga gagcgacttt tttatcagta atgccgtgaa tataaaaaga
    22321 atgtttaccg tttattagat gacagattat gcgtctttcg ctaaagtttc cggtcaaatt
    22381 agtcgtttac ttgttacaca gcttagattt cttttgctcg ggagaggcat gtcaggatcc
    22441 aacactgcaa tcagccgtcg tcgtttactg caaggcgcgg gcgctatgtg gctattgagc
    22501 gtaagtcagg tcagcctggc tgcggttagc caggtcgtgg cggtgcgcgt ctggcctgcg
    22561 tccagctaca cccgcgtgac ggtagaatca aatcgtcagc tgaaatataa gcagttcgcg
    22621 ttgagtaacc ctgaacgcgt ggtggtggat atcgaagatg taaacctgaa ctcggtactc
    22681 aaggggatgg cggcacaaat tcgtgcagac gacccgttca tcaagtcggc gcgcgtcggg
    22741 caatttgacc cgcaaaccgt acgcatggtt tttgaattaa agcaaaacgt aaaaccgcag
    22801 ctgtttgccc ttgcgccggt cgccgggttt aaagagcgtc tggtgatgga cctctatccg
    22861 gccaatgcac aggatatgca ggacccgctg ctggcgctgc tggaggatta caacaaaggc
    22921 gacctcgaaa agcaggtgcc gccagcgcaa agtggtccac aaccgggtaa agcagggcgc
    22981 gatcgtccga ttgtcattat gcttgaccct ggccacggtg gcgaagattc cggtgcggtg
    23041 gggaaataca aaacgcgcga aaaagatgtg gtattgcaaa tagctcgccg tttgcgctct
    23101 ctgatcgaga aagagggcaa tatgaaggtg tacatgacgc gcaatgaaga catcttcatt
    23161 ccgttgcaag tgcgcgtagc aaaagcccag aaacagcgtg ctgacctgtt tgtctctatc
    23221 catgccgacg cctttaccag tcgtcagccg agcggttcct cggtgtttgc gctctcaacc
    23281 aaaggcgcaa ccagtactgc ggcaaaatat ctggcacaaa cccagaacgc ctcggacttg
    23341 attggtggcg tgagcaaaag cggtgaccgc tatgtcgacc acaccatgtt cgatatggtg
    23401 cagtcgctga ctattgctga cagccttaag tttggtaaag cggtactgaa taagctcgga
    23461 aaaatcaaca agctgcataa aaatcaagtt gaacaggccg ggtttgccgt actaaaggca
    23521 ccagatattc cctccattct ggtcgaaacg gcgtttatca gtaacgttga ggaagagcgt
    23581 aaactgaaaa cggcgacttt ccagcaggaa gttgcggagt ctattcttgc ggggattaaa
    23641 gcgtattttg ccgatggggc aacgctggcg agaaggggat gataaaaagg cgctgaatgg
    23701 cgcttttttt atgggagatc agaaacaaaa aaacacccgt tagggtgttc aataattggt
    23761 tgcgggggcc ggatttgaac cgacgacctt cgggttatga gcccgacgag ctaccaggct
    23821 gctccacccc gcgtcaccgt actgctttta cttcatcaaa tttgattggt tgcgggggcc
    23881 ggatttgaac cgacgacctt cgggttatga gcccgacgag ctaccaggct gctccacccc
    23941 gcgtccgtgg atgcgcacta tactccgctc gcgttttcat gcaacctttt ttttaccccg
    24001 atcacggatt tagcatgatt ttgaacaaaa acaaaccact aaagcttatt ttttgtgcga
    24061 aatttgcgtc agggagaggc tgctcatttc attaaacgcg gcggtttgtt atcttcgttg
    24121 cgccttattt tttaacctga agaagagaac aatgaaagga cgttgggtaa agtaccttct
    24181 tatgggcacg gttgtggcaa tgcttgccgc ctgctcttcc aaaccaaccg atcgcggaca
    24241 gcaatataaa gacgggaaat ttacccagcc tttctctctg gtgaaccagc cagatgccgt
    24301 tggcgcgccg attaacgccg gtgattttgc cgagcaaatt aaccatatcc gtaattcgtc
    24361 accgcgtctg tatggcaacc agagtaatgt ttataacgcg gtgcaagagt ggctgcgcgc
    24421 aggcggtgat acccgcaata tgcgccagtt cggcattgat gcctggcaga tggaaggtgc
    24481 cgacaactat ggtaacgtgc agtttacagg ctattacacg ccggtaattc aggcgcgcca
    24541 tacccgccag ggcgagttcc agtatcctat ttaccgtatg ccgccaaaac gtggtcgtct
    24601 gccgtctcgt gcggagatct acgccggggc attgggcgat aaatacatcc tcgcttacag
    24661 caactctctg atggataact tcattatgga tgtgcagggt agtgggtata tcgactttgg
    24721 tgatggcagt ccgcttaact ttttcagcta tgcagggaaa aacggtcatg cctatcgcag
    24781 cattggtaag gtgctgatcg accgtggcga agtgaaaaaa gaagatatgt cgatgcaggc
    24841 gattcgtcat tggggcaaaa cacacagtga agccgaggtt cgcgagctgc tggaacagaa
    24901 cccgtctttc gtcttcttta aaccgcaatc ttttgcaccg gtgaaagggg caagtgcggt
    24961 gccgctggtt ggccgtgctt ctgttgcctc cgatcgttca attattccgc caggtactac
    25021 cttgctggca gaagtgccgc tgctggataa taacggcaaa tttaacggtc agtacgaatt
    25081 gcgtctgatg gtggcgttgg atgtcggggg cgcaatcaaa ggccaacact tcgatatcta
    25141 tcaagggatc gggccggaag ccggacaccg cgcaggttgg tacaaccact atggacgggt
    25201 ttgggtgctg aaaacggctc cgggcgcagg taacgtcttt agcggctgat gtgatattct
    25261 gtatgccgtt tttacggata tgacagggtg aggggtaacc tcacatcctg attattgact
    25321 aagtgcgcgt tgttcatacc ggatgcggcg tgaacgcctt atccggccta caaatcatgc
    25381 aaatccaaaa tattgcagga gttatgtagg cctgataagc gtagcgcatc aggcatttat
    25441 gcgtttaggg tgagggataa cctcgccctt tttaattctg aggtgctatg tctgtggtaa
    25501 ttagtgatgc atggcgtcag cgttttggtg gcacagcgcg tctgtatggt gaaaaagcgt
    25561 tgcaactgtt tgctgacgcg catatttgtg tggttggtat cggtggtgtc ggttcctggg
    25621 cggcggaagc gctggcgcgc acggggattg gcgcaatcac gcttatcgat atggatgatg
    25681 tgtgcgtcac caataccaat cggcaaattc acgctctgcg cgataacgtc gggctggcaa
    25741 aagcggaagt catggcggag cgcattcgcc agattaaccc ggagtgccgt gtaacggtgg
    25801 tggatgattt cgtgacgccg gataacgtag cgcaatatat gagtgccggt tattcgtacg
    25861 tgattgatgc cattgatagt gtacggccca aagcggcgct gattgcttat tgtcggcgca
    25921 ataaaatccc gctggtcacg accggtggcg cgggtgggca gattgacccg acgcagattc
    25981 aggttaccga tctggcgaaa acgattcagg atccgctggc ggcgaagttg cgcgagcgcc
    26041 tgaaaagcga ttttggcgta gtgaaaaaca gtaaaggtaa gctcggcgtg gattgcgtgt
    26101 tttctactga agcgctggtg tacccgcagt cagacggtac ggtgtgtgcg atgaaagcca
    26161 cggcagaagg gccgaagcgg atggattgtg catctggatt tggcgcggca acgatggtga
    26221 ccgccacctt tgggtttgtt gcggtttctc atgcgctgaa gaagatgatg gcgaaagcgg
    26281 cgcgtcaggg ttaaacaatc aggtcggatg agacgcgaac gtcttatccg acccggttct
    26341 gtgcttaaac ctgcttcgtc gcagcgataa tcgcctcgct taacgcattt aacccctggc
    26401 tgcgtgaggc gctaagctgc gcacgtaatc ccagctcatc aaacaatgcc agcggcgact
    26461 gtgcctgtaa ttcggcggcg gttttcccct caacggcagt caacaacacc gccaacaggc
    26521 cgcgcacaat gcgcccttcg ctgtcgccaa agaagtgcat tttgccgttt tcagccacgg
    26581 tatatcccag ccagacgcgg ttttcgcatc cggcaatctc tttagcctgc gcttttaact
    26641 cgtctggcaa agccggaagc tgtttcccca gcatgatcaa ctggcgatat ttatcttccc
    26701 attgcgacaa cggtgcgaag gtattgcgta acgtttctgc ggttacggtt gtgccgaacg
    26761 gatgtccggc gaattgcggg tttgtcatta atccaccaat aattccagcg cgcggtcaac
    26821 ggcattcacc agcgcatcca catcactctt tgtattatat ggcgcaaatg aggcgcgcag
    26881 tgtgccggtt acgcctaatt ctgccagtag cggctgagcg caatgttgcc cggcccgcaa
    26941 ggcaataccg tactccgcca gtagcgtcac catatcgctg tgatgaacgc cagcaaaatc
    27001 aaaggccagc aggctggaat cctggcagcg gaatgaacga aagcctggac gtttcgccag
    27061 cgcttcttct gcaagcgttg ctaagctacg gctccagctt tcggcctggt tgatatcgta
    27121 atctgccagc cattccagcg ccgcgcttaa tcctatgacg ccagcgacat ttggcgttcc
    27181 tgcttccagt ttccacggcg cagattgagt ggtgaaaccg tcaaaactca cttcgtgaat
    27241 cattttgccg ccacccagcc agggcgacat cgcttccagc agttctgatt tgccatacag
    27301 cacgccgata cccgtcgggc catacagttt gtgaccagag aaagcataga aatcgatatc
    27361 cagttgctga acatccgcgg ggaaatgcac cgccccctga gcaccatcaa ccatcaccac
    27421 catcccggct gaatgagcaa aggtaatcgc tcgcgccaga tccgggcaac cgccagtcac
    27481 gttcgacatc tgacccaacg ccagaatccg actacgggga gtaatcagtt ctggcaacaa
    27541 atcgacatcc ggcagtcgct gcgcattaag cggcaatttc accactttgg ctctagtttg
    27601 ttgggcgacc atcagccagg ggacgaggtt agcgtggtgt tctgccacgc tgacaataat
    27661 ctcatcgccc ggttgcagac gcggacgcgc atagcattgt gccaccatgt tgatggattc
    27721 agtggtgccg cgcgtccaga cgatagtttt atcatccggt gcattcagta attgcgccac
    27781 tttctctcgt gcagcttcat aacgcgcggt caggcgttgg gcttcggcaa actggctgcg
    27841 atggacgttt ccggcgctca ggctgtaaaa ctgtcgagtg gcttcaacca cggcttcagg
    27901 tttaagcgcg gtcgcggcgc tgtcgagata gacgcccgca tcctgtagtg cgggaaactg
    27961 ggcgcgaaac tgcgcgggat taaaaacgtt catggtactc ctcggcttga tgcaccgatc
    28021 gtgacgcaaa attcactttg atacaaggag agtgctaacc atcagaatta tctagatatc
    28081 ctggcgaagc cgcgtaagta acatatgctg aatagtgtta ggcgttaatt ttagcctgtt
    28141 aatcaatact attaagattt attaatcgct ttaaggagaa gatgatgaaa aagactgccg
    28201 caattatttc tgcctgtatg ctgacttttg ccctgagcgc ctgttccggt tcgaactatg
    28261 tgatgcacac caatgacgga cgtaccatcg tctctgacgg caaaccacag actgataacg
    28321 ataccggtat gatttcgtat aaagacgcta atggcaacaa acagcagatc aaccgtactg
    28381 acgtgaaaga gatggtcgaa ctggaacagt aattcgcgat cgcaaggtaa aaaaaagcac
    28441 cgcaattagg cggtgctaca ttaatcacta tggacagaca gggtaaatgt acaggaagtg
    28501 aaaaaaggta gctttgctac catggtctga atcgcagacc aattgcaaac acaacaacac
    28561 aacatcacaa ccgtaagcca aaagttcacc agaatacgca ttccgataaa acttttcgtt
    28621 ccggctcagg aagtgccgcc actataggta tttgctggta gaagctcaac ggacaattta
    28681 taatggctca gattaaaaaa actaataggt tacacagtgt gatctaattg ttaaattcat
    28741 ttaacatcaa agtttaaaag ccatgtctaa acgattacca ccgctaaatg ccttacgagt
    28801 ttttgatgcc gcagcacgcc atttaagttt tactcgcgca gcagaagagc tttttgtgac
    28861 ccaggccgca gtaagtcatc aaatcaagtc tcttgaggat tttttggggc taaaactgtt
    28921 ccgccgccgt aatcgttcac tcctgctgac cgaggaaggg caaagctatt tcctcgatat
    28981 caaagagata ttttcgcaat taaccgaagc gacgcgtaaa ctccaggccc gtagcgccaa
    29041 gggggcgttg acggtcagtt tactccccag tttcgccatt cattggttgg ttccgcgact
    29101 ttccagcttt aattcagctt atccgggaat tgacgttcga atccaggcgg ttgatcgtca
    29161 ggaagataag ctggcggatg atgttgatgt ggcgatattt tatggtcggg gcaactggcc
    29221 ggggctacgg gtggaaaaac tgtacgccga atatttattg ccggtgtgtt cgccgctact
    29281 gctgacaggc gaaaaaccct tgaagacccc ggaagatctg gctaaacata cgttattaca
    29341 tgatgcgtca cgccgtgact ggcagacata tacccgacag ttggggttaa atcatatcaa
    29401 cgttcagcaa gggccaattt ttagtcatag cgccatggtg ctgcaagcgg ctattcacgg
    29461 gcagggagtg gcgctggcaa ataacgtgat ggcgcaatct gaaatcgagg ccggacgtct
    29521 tgtttgcccg tttaatgatg ttctggtcag taaaaatgct ttttatctgg tttgtcatga
    29581 cagccaggca gaactgggta aaatagccgc ctttcgccaa tggatcctgg cgaaagccgc
    29641 tgctgaacaa gaaaaattcc gctttcgtta tgaacaataa tttacgtagg gtacgaccat
    29701 gaccagccgt tttatgctga ttttcgccgc cattagcggc ttcatttttg tggcactggg
    29761 cgcttttggt gcgcatgtgt taagtaaaac catgggggcc gttgagatgg gctggatcca
    29821 aaccggtctc gaataccagg cgtttcatac gctggcgatc ttaggtctgg cggtggcaat
    29881 gcagcgtcgc atcagtatct ggttttactg gagtagcgtt ttcctcgcgt taggcacggt
    29941 gctgttcagc ggcagccttt attgcctggc gctgtcccat ctgcgtttgt gggcgtttgt
    30001 cacaccggtt ggcggcgtga gcttccttgc gggctgggcg ttaatgttag ttggtgctat
    30061 ccgtttaaag cgcaagggcg taagtcatga ataaggttgt attgctgtgc cgtccgggct
    30121 ttgaaaaaga gtgcgccgca gaaattaccg ataaagccgg ccagcgggaa attttcggtt
    30181 ttgcccgcgt gaaagagaat gcgggttatg tcatttatga atgttatcaa cctgatgatg
    30241 gcgataagtt aatccgtgag ctgccgttca gttcattaat ttttgcccgc cagtggtttg
    30301 tggtggggga actcctgcag catttgccgc cagaagatcg tattaccccc attgtcggca
    30361 tgttacaggg tgtagtagag aagggcggtg aactgcgtgt tgaagttgcc gataccaacg
    30421 aaagcaaaga gttgctgaaa ttctgccgta agttcactgt tccgctacgc gctgccttgc
    30481 gcgatgctgg ggtgctggcg aactatgaaa cgccgaagcg tccggttgtg catgtattct
    30541 ttattgcacc aggatgctgc tataccggtt actcatacag caacaataat tcgccgttct
    30601 atatgggcat tccgcgcctg aaatttccgg cagaggcgcc gagtcgttcc acgctcaaac
    30661 tggaagaggc atttcatgtg tttattcctg cggatgagtg ggatgaacgc ctggcgaacg
    30721 ggatgtgggc ggtggattta ggcgcttgcc ctggcggctg gacctaccaa ctggtgaagc
    30781 gcaacatgtg ggtttattcc gtcgacaacg gcccgatggc gcaaagtctg atggataccg
    30841 ggcaggtgac gtggctgcgg gaagacggtt tcaaattccg tccgacacgc agcaatatct
    30901 cctggatggt atgcgatatg gttgaaaaac cggctaaagt tgcggcattg atggcgcagt
    30961 ggctggttaa tggctggtgc cgtgaaacca ttttcaatct caaactgccg atgaaaaaac
    31021 gctacgaaga agtatcacac aatctggcgt atattcaggc acagcttgat gaacatggca
    31081 taaatgctca gattcaggcg cggcagttgt atcacgatcg cgaagaagtg acggtgcacg
    31141 tccgccgcat ctgggctgcg gtgggtggtc gtcgcgacga gcgataacag gcaaggccgg
    31201 ggcgtcgtct ccggcctgct accctttccg tcaggctgtt accaaagaag ttgcaacctg
    31261 gcgctcatca gaaataatgt gcgttacctc atccagatac ccgatgcggg cttcccctga
    31321 acgattaaat ttacttttat caatcaataa caacgattgc gcggcacgtt ttaatagcat
    31381 cgatttgtaa tcagcgttga tcgcattgga gtcccacagt gcgccgctgc tatcgatccc
    31441 ttcacaagaa aaaataaaca gatcgatttc cagcgattta agttgggaaa tcagcgaggg
    31501 attgacgtaa cagccatatt tgcgctcaag tgtgccgccg gaactgatca gttgaatgcg
    31561 ttcgcgttta ccgagttcat ggcaaatcgg atggctattg gtgaagacct gaatgttgat
    31621 gtcaggcaac tggcgtgcca gataccagca agttgaactg gcgtctaagg ctatcaccat
    31681 cctttcttca atccacgcga gcgcctcgcg cgcgatatct gctttatgcg cataatggct
    31741 tttcagcctg atgtgaaaga gagcgctact gtcttgattt tgacggtgga tatatttagc
    31801 gcgtccatga ttgcgcagaa ttttaccctg cgtctgtaat tcattgagat cgcgacgaat
    31861 ggtttcttta ctgaccttta gctgttcaga gagagcttcc gtggtcaggc tggtatggtt
    31921 cagcagcagg tcgactatcg cttgctggcg tgccgctttc atatttttca ccccatcatt
    31981 cgataaccgc ccgcgtcatg cgggcgtacc ggtatgagat tacggtgtta cccctttttt
    32041 taaaagaata ttcccgtact tcgctcgttc gcctgtgata acgatcgcaa aggctttttg
    32101 cgcccgttca taaaacgcaa aacgattgat gcggatgatg tcaggacacg gggcttgtag
    32161 tgaaagcgca ttacggtaac gtcgttctac ttcaggatcg agagtgtcac cctctaccgc
    32221 cgccatcatc accagcggcg gggcataact gtccagctca aataacggga taatcgcctg
    32281 gagcaagtcg ctcaccaaca ggccatcagc gcggatcacc tgcggtccca tcgaatgggc
    32341 gggaaagtga gcatcggaaa aaataatttc atctccatgt cccatctctg ccagcacttt
    32401 caatagttcg ggagaaatta acggcgaaat tgttttcagc atttcacact tcctctataa
    32461 attcaggttc agtttgcggg tagaaataac ggtactgata atgaatctgt gcacgggctt
    32521 cttccgggct gttaaattcc cctacgccat accaaccgaa cagcgcagct cctgcgacgg
    32581 tcgtttcggc atcgtcgaga acttttaccg gaatatcaag catattggct ttaatctgat
    32641 tccacaatgt gttgcgactt cctccaccga ctaacaataa ttcataggct ttaaagtgcc
    32701 cgattttttc cagcatctgt agattgcgct gtaattgcgc agttaaccct tccagcgccg
    32761 cgcgatagaa atgcccccgc gtggtattaa gcgtcactcc ttgccagcca gcgttctgac
    32821 acgacaataa atcacactgc attttcacac catccgcgcc aggtgcgatc agacgagctt
    32881 cttcaatcaa catttgccag ggtgtttcag ccgtccagaa cagttttctc acccattcca
    32941 gcacgccgga tgccagccat tgcatacctg ggttatacaa ccatgcctgg ctatctagtt
    33001 cgcaggtgga accggcgtac tgacttaaca gcgaagtatc aacctaggcg ctgcgaacca
    33061 ttaaaatttc ccatgtaccg gaggagagca cgggttcatt ttgttcggca ccagcgccaa
    33121 aaagggcgaa ctgggtatcg tgacctgcgg aaatcaccgg tatgccaacg ggtaagccga
    33181 gcattgctgc ggcgctgttc tgtagcgtac caatctgttc acccgcttcc accagacgag
    33241 ggaagagtcg gcgtggaata ccggtggctt gtaaaatttg cggactgaaa tcgcgttgct
    33301 ggatatccag catctggctg gttccggcta tcgtgatatc agtagtgaat tcgccggtta
    33361 aacggtggtt aatcagcgac gaaatgaaga gccaggcgtg cgcgcgttcc agcagttgtg
    33421 gatgattttc tttcaaccac accaacttat ataacgtatt gaaactaaag gctccgacgc
    33481 cagaaatagc ctgcaaccgc tgtgcggaga ttaaccgttc aatattgtcc ataaccgctg
    33541 ctgttcgcgg acatttccag ctaatgatcg gatagagcag attgccttgc ttatctacca
    33601 gagcgccatc cacaccaaag gtggtgacgg cgataccgcg gatgtggcat tcagtcagtt
    33661 cactattgat ttgccgacag caatcagcaa agcgttgcaa aatggcagtc taaagaccac
    33721 tggtgccagg tgttgttttc catcgcgata tcgctggcat taggcgttga ggcgcgggca
    33781 acaatttttc cctgccgatt aaccgcgatg gccctgacat tggtcgcgcc acagtcgagt
    33841 accaggataa cttcttgttt cataatcgct cctgcaatat agccggataa cattgtttat
    33901 ccggctaacc actcttgctt gttcaggccc ggtagccgga gcgaccgggc atcacatcag
    33961 ggtgtaatgt attaacgctt gtacaacgga ccgtagttct ggcaagcgcg gtaatcctgg
    34021 ccttcaatat ccatgccgtg cgcagcccag gcagaaggac gatacacttt ggtctcttca
    34081 acgttgtgca tacataccgg gatacgcagc atggaggcaa gggtgataaa gtctgcgcca
    34141 acgtggccga tggtcagaac tccgtggttg gcaccccagt tcgccattac cgaatacaca
    34201 tccgtaaacg gccctttacc agtgaggcgc ggtgcaaacc aggtggttgg ccaggttgag
    34261 ttggtgcgtt tgttgaggat gtcatgcaca tccttcggca attccacgct ccagccttcc
    34321 gcgatttgca gtaccggtcc caggcctttg atgatgttga cacgagtcat ggtgaacggg
    34381 acgccgcctt cggtaaggaa gcgggaagag taaccgccgc cacggaagta ttcgtggatc
    34441 gccgggcacc attcggtagc ggcgaggcaa gcgtcagcct cttgctgaga gatttcccag
    34501 tgcggcttca tcgtcgggtt accttcgctg tcgcgttgtt tacaggaacc gtccagcgca
    34561 gcagaacctg agttgatcaa atggatgatg ccgtgttctg ccagtccgtc cagtttatgc
    34621 cccgttacac gctcaattgc ttctggtgac cagtaggtac gcacatcggc aaatacctga
    34681 gcggtgccgg tgagctggtg acccattagc attgccacgc cgttaagact gtcgttttcg
    34741 gtcgccacga caaagggttc gcgcacgcca ttccagtcaa atgaactgtt gaggatcgct
    34801 tcggcggtgt caccattggg atattgatcg gtccagtgac gttgcccctg gaagcccgca
    34861 gcgatggcgt tgtagccaag tgattcttcc acgcgaccaa tatcggccag tttgctgttg
    34921 ccttgcatca tgtcgcggat acacatcgcc atcagtaaac tttcgcgcag aactgcgcgg
    34981 ctttgctcgg cattgcgttg atactgtttg ttattttcat cttcgccata gcggaagttt
    35041 ttatcagccc aggccagtgc catttccagt tcggcttcgt cgtaaatctt ctgatcgata
    35101 cggcgacgca gttcggtcat atccaccgcc tggactttca ttcccagcca ggattcaaag
    35161 aagttgtgat caacaataga accggcgata cccatcgaaa cgccgcccag cgacagatag
    35221 cttttacctt tcatgctggc gacggccaaa ccggcgcggg caaagcgcag cagtttttct
    35281 tcaacatcgg caggaatcga tgtgtcatcg gcatcctgaa cgtcatgacc gtaaatggag
    35341 aatgccggga tgcctttctg gctgtgagct gccagagccg ctgccaggta aacagcgccg
    35401 gggcgttcag tgccgttaaa gccccaaatg gctttcgggc gggttggatc catgtcgatg
    35461 gtttcactgc catagcacca gcaaggcgtt accgtaatgg tgaggcctac attctggctg
    35521 ctgaattttt cttcgcaagc agcggcttca gccatacccg cgatacaggt atcggaaatg
    35581 acacactcga cggcagctcc gcaggtatgg cgcagtttct cggtcagcag tgcggccgta
    35641 gctttcgcca tattcattgt ttgttcttca agcgactcac gaacacccat gcgacgaccg
    35701 tcaataaccg ggcggatacc aattttcggt aagctgattt ttttcattac agattcctca
    35761 ctttattcgg aaaatatgtt cagttagttg ccgtttgaga acggaaacgg gcaaagataa
    35821 agatgaccgc gaagcagagt gcggggatca gttcagcagt ggggatgttg cccgccgcgt
    35881 cactgacaaa acccatgacc ggagtgacaa taccgccgcc aataatggtc ataacgatga
    35941 aggacgaacc gtatttggtg tcctggccga gattcttaat gcccagcgag aagattgttg
    36001 ggtactgaat cgacataaag gcgctgcata aagtcagggc tattaagccc acatgaccgc
    36061 cagcgaaggc tgagatcagg cacagtgcca tagcgattaa tgcgtaggcg gccaggactt
    36121 tgtgtggtgc gaagcgactg atgagccagg taccggtgat acgaccaata aagaagcaca
    36181 ccatggttcc ggttaaatag ttagcggcaa agcctgcagt catacctgga atttcttcta
    36241 cagcgtagcg aatcaaatag ctccagcagg ccgtttgtgc gccgacatag cagaattgcg
    36301 ctaataccgc ccagcgccag tgacgaatac gcgccagacg agaaagcgat gcggagaacg
    36361 atccttgttt ggcgtcactg tgattatcac tctgcaatgc cgggaatttc gtcagcatga
    36421 tcagcagggc gaccagtaac acgatagcca cgatgatcat ataaggtgtc tgtaccgata
    36481 atactaggct gtgtttatac gcactcaatt gctctggaga cattttatcg agaacgtctt
    36541 gcgattgatg tggcacgtta gacaaaataa gactttgccc aaagacaacc gcgataattg
    36601 cgccaa
//
LOCUS       DAJTYF010000024        36519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTYF010000024 DAJTYF010000000
VERSION     DAJTYF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.024"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(155..1390)
                     /locus_tag="OO590_001365"
     CDS             complement(155..1390)
                     /locus_tag="OO590_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6636796.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1537..1613)
                     /locus_tag="OO590_001366"
     tRNA            complement(1537..1613)
                     /locus_tag="OO590_001366"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1577..1579),aa:Pro,seq:tgg)
     gene            complement(1656..1742)
                     /locus_tag="OO590_001367"
     tRNA            complement(1656..1742)
                     /locus_tag="OO590_001367"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1706..1708),aa:Leu,seq:cag)
     gene            complement(1763..1838)
                     /locus_tag="OO590_001368"
     tRNA            complement(1763..1838)
                     /locus_tag="OO590_001368"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1803..1805),aa:His,seq:gtg)
     gene            complement(1897..1973)
                     /locus_tag="OO590_001369"
     tRNA            complement(1897..1973)
                     /locus_tag="OO590_001369"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1937..1939),aa:Arg,seq:ccg)
     gene            complement(2076..3461)
                     /locus_tag="OO590_001370"
     CDS             complement(2076..3461)
                     /locus_tag="OO590_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6636797.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3652..4392)
                     /gene="rffM"
                     /locus_tag="OO590_001371"
     CDS             complement(3652..4392)
                     /gene="rffM"
                     /locus_tag="OO590_001371"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6636798.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4395..5747)
                     /gene="wzyE"
                     /locus_tag="OO590_001372"
     CDS             complement(4395..5747)
                     /gene="wzyE"
                     /locus_tag="OO590_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6636799.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5744..6823)
                     /gene="rffT"
                     /locus_tag="OO590_001373"
                     /gene_synonym="wecF"
     CDS             complement(5744..6823)
                     /gene="rffT"
                     /locus_tag="OO590_001373"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6636800.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6820..8070)
                     /gene="wzxE"
                     /locus_tag="OO590_001374"
     CDS             complement(6820..8070)
                     /gene="wzxE"
                     /locus_tag="OO590_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6636801.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRFGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8072..9202)
                     /gene="rffA"
                     /locus_tag="OO590_001375"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8072..9202)
                     /gene="rffA"
                     /locus_tag="OO590_001375"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6636802.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9207..9881)
                     /gene="rffC"
                     /locus_tag="OO590_001376"
                     /gene_synonym="wecD"
     CDS             complement(9207..9881)
                     /gene="rffC"
                     /locus_tag="OO590_001376"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6636803.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9859..10740)
                     /gene="rfbA"
                     /locus_tag="OO590_001377"
     CDS             complement(9859..10740)
                     /gene="rfbA"
                     /locus_tag="OO590_001377"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6636804.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10759..11826)
                     /gene="rffG"
                     /locus_tag="OO590_001378"
     CDS             complement(10759..11826)
                     /gene="rffG"
                     /locus_tag="OO590_001378"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6636805.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11826..13088)
                     /gene="wecC"
                     /locus_tag="OO590_001379"
     CDS             complement(11826..13088)
                     /gene="wecC"
                     /locus_tag="OO590_001379"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6636806.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13085..14215)
                     /gene="wecB"
                     /locus_tag="OO590_001380"
     CDS             complement(13085..14215)
                     /gene="wecB"
                     /locus_tag="OO590_001380"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6636807.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAVNYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14271..15317)
                     /gene="wzzE"
                     /locus_tag="OO590_001381"
     CDS             complement(14271..15317)
                     /gene="wzzE"
                     /locus_tag="OO590_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6636808.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15329..16432)
                     /gene="wecA"
                     /locus_tag="OO590_001382"
     CDS             complement(15329..16432)
                     /gene="wecA"
                     /locus_tag="OO590_001382"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6636809.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16672..17931)
                     /gene="rho"
                     /locus_tag="OO590_001383"
     CDS             complement(16672..17931)
                     /gene="rho"
                     /locus_tag="OO590_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6636810.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18016..18117)
                     /gene="rhoL"
                     /locus_tag="OO590_001384"
     CDS             complement(18016..18117)
                     /gene="rhoL"
                     /locus_tag="OO590_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6636811.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18258..18587)
                     /gene="trxA"
                     /locus_tag="OO590_001385"
     CDS             complement(18258..18587)
                     /gene="trxA"
                     /locus_tag="OO590_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6636812.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18718..19983
                     /gene="rhlB"
                     /locus_tag="OO590_001386"
     CDS             18718..19983
                     /gene="rhlB"
                     /locus_tag="OO590_001386"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6636813.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20117..21601
                     /gene="gppA"
                     /locus_tag="OO590_001387"
     CDS             20117..21601
                     /gene="gppA"
                     /locus_tag="OO590_001387"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6636814.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21648..23669)
                     /gene="rep"
                     /locus_tag="OO590_001388"
     CDS             complement(21648..23669)
                     /gene="rep"
                     /locus_tag="OO590_001388"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6636815.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAVKRGK"
     gene            23756..24037
                     /gene="ppiC"
                     /locus_tag="OO590_001389"
     CDS             23756..24037
                     /gene="ppiC"
                     /locus_tag="OO590_001389"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6636816.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24083..25558)
                     /gene="ilvC"
                     /locus_tag="OO590_001390"
     CDS             complement(24083..25558)
                     /gene="ilvC"
                     /locus_tag="OO590_001390"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6636817.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25708..26598
                     /gene="ilvY"
                     /locus_tag="OO590_001391"
     CDS             25708..26598
                     /gene="ilvY"
                     /locus_tag="OO590_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6636818.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26595..28139)
                     /gene="ilvA"
                     /locus_tag="OO590_001392"
     CDS             complement(26595..28139)
                     /gene="ilvA"
                     /locus_tag="OO590_001392"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6636819.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28142..29992)
                     /gene="ilvD"
                     /locus_tag="OO590_001393"
     CDS             complement(28142..29992)
                     /gene="ilvD"
                     /locus_tag="OO590_001393"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6636820.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(30057..30986)
                     /gene="ilvE"
                     /locus_tag="OO590_001394"
     CDS             complement(30057..30986)
                     /gene="ilvE"
                     /locus_tag="OO590_001394"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6636821.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31006..31269)
                     /gene="ilvM"
                     /locus_tag="OO590_001395"
     CDS             complement(31006..31269)
                     /gene="ilvM"
                     /locus_tag="OO590_001395"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6636822.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31266..32912)
                     /locus_tag="OO590_001396"
     CDS             complement(31266..32912)
                     /locus_tag="OO590_001396"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6636823.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32915..32965)
                     /locus_tag="OO590_001397"
     CDS             complement(32915..32965)
                     /locus_tag="OO590_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636824.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33051..33149)
                     /gene="ilvL"
                     /locus_tag="OO590_001398"
     CDS             complement(33051..33149)
                     /gene="ilvL"
                     /locus_tag="OO590_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6636825.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33501..35021
                     /locus_tag="OO590_001399"
     CDS             33501..35021
                     /locus_tag="OO590_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR6636826.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35046..35384)
                     /gene="maoP"
                     /locus_tag="OO590_001400"
     CDS             complement(35046..35384)
                     /gene="maoP"
                     /locus_tag="OO590_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6636827.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35503..36342
                     /gene="hdfR"
                     /locus_tag="OO590_001401"
     CDS             35503..36342
                     /gene="hdfR"
                     /locus_tag="OO590_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6636828.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36438..36513)
                     /locus_tag="OO590_001402"
     tRNA            complement(36438..36513)
                     /locus_tag="OO590_001402"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36478..36480),aa:Trp,seq:cca)
BASE COUNT         8920 a         9993 c         9199 g         8407 t
ORIGIN      
        1 ccaccgcacg caaaggcttt gccggatacg atcacccaaa ccagtatcca cagaacgctg
       61 caatttattg aatttgcacg atcatgtagg ccggataagg cgtttacgcc gcatccggca
      121 atcaaccgca ggcggcttcc aaatggccgc ttttttactt attcaccacc atcaaatgtg
      181 cctgcataat gtcactggcc gggcgaccgt gcgccagcaa atcagccatt gctttaagat
      241 atggcggtag atggcggaaa taacgctgat acccggcaca caaataattc agccccggtt
      301 tgccgctggc atcgagcatg aagcggtgtt tcgggcagcc tccccaacat gcttttaaca
      361 cgttacaact gcgacactgc gccggtaatt gcttaaattt atcttcacca aacacctgct
      421 gttgcgggga atcgatcatt tctgcaattg tttgctgatg catattcccc agccgatatt
      481 gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca atcaccgagc
      541 gcccacaggt tggctgatga tggcaaaccg cacccggcgc accgacaaaa ttggcaaacg
      601 cccattcgat attcatcacg aaaatcttgc cgacgtcgcg tttgatccag tggtcaaaaa
      661 tcgccaccag aaattcacca aattcatcgg ggcgcacaga ccattccgtc agttccccct
      721 gaatatcacc aggcgcatgt agtttcagtc catcgcggac agctgtttca tcagccaggc
      781 gctcgaccac cggaataaac tggatgaatt cgactcccgc atcgcacaaa aaatcataaa
      841 cctgtaacgg ttgctgcgcg ctggtgcggt tgacgcagac cagcacgtta tagtcgacat
      901 gatgtttttg caggagcgtc agggcacgca tcaccagttt atgcgtgggt ctgccgcctt
      961 tggtcacgcg atattgattg tggatctcag ccggaccatc cagcgataac ccaacaagaa
     1021 aattattttc tgccagaaat gcacaccatt cgtcatcgag cagcacgccg ttagtctgga
     1081 aactgttact tatcttcctg ccagcaccat atttcgcctg tagcgccacg gcacggcggt
     1141 aaaactccag cccgagtagc gttggttcgc cgccctgcca ggtaaaagcc acttcgtttt
     1201 gcagttcgct ggcggcgata tagtgacgga tatacgcttc cagcgtgtca tcgtccatat
     1261 gcgtgactgg cttttcgcgg taaagggatt gtttttcgag ataaaaacag tagtcacagt
     1321 tcagattaca atcggaaccg ctcggtttcg ccatcacatg aaaagcacgc gttggaacct
     1381 gttgcagcat ggtcgctcct tgcctgaagt catccttcaa tacgttaagg gcgaaatcat
     1441 accgtggggc aatgagttat tacgggcgtt tattgcgaag ggagggtgac gaaatgcaca
     1501 gaaaacaaaa aaccccgccg aagcggggtt caaaattggt cggcgagaga ggattcgaac
     1561 ctccgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgaaatact
     1621 gctttttgaa tttttagttc aattctttaa agtcgtggtg cgaggggggg gacttgaacc
     1681 cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc
     1741 gcattgtcac aacttctaat aatggggtgg ctaatgggat tcgaacccac gacaactgga
     1801 atcacaatcc agggctctac caactgagct atagccacca ctacaaatct tgttacgcgg
     1861 tattactacc accgcagctc aagcgccggg actaaatggc gcgcccgaca ggattcgaac
     1921 ctgagacctc tgcctccgga gggcagcgct ctatccagct gagctacggg cgcttagcgc
     1981 cgttgcgggg gtggataata cggacttccc acccctctgt ctagtccctt tttaaataaa
     2041 ttgctcgttt ggttacggtt tgtgcgtttt gctgcttatt cctccagttt atgcgcttta
     2101 ccgtggcgat taagcccaaa aactttataa atcgccgtca ccgccagcat aaagatgatg
     2161 ccgacaaaca gcgacatacg cgtatcttca ttaaagtaca tgccgatcaa aacgcaaatc
     2221 aggaatgcca ttgttatgta gttggcccac gggaacagga ttgagcggaa cggatggctg
     2281 gcaatcgccg ctttatgtgc acgccgaaaa cgcagctggc ttatcaatat cacaaaccat
     2341 ggcaccatcc ccggaagcac gctggcactg tagacgtaga caaacacacg ctgcggattg
     2401 ggaatgatgt agttcaggca tgagccaatt agcagaatag caatagatac tgccacaccc
     2461 gcaaccggta cgccgtgacg ggaaactttt gccattgccg ccggtaactg acggtttttc
     2521 gccagtgcgt agagcatacg tccgcaactg tacatgccgc tgttacagcc agagagcgca
     2581 gccgtcagca ccacaaagtt gataatgccc gccgctgcgg taataccgat tttggcaaaa
     2641 gtcagtacga acgggctgcc gttgctgcca atttcattcc acgggaagat ggtgacgata
     2701 acgaaaatcg cgcctacgta gaaaatcagg atccgccaca gcactttgcc tacggcactg
     2761 cgcagcgtca cctgcggatt cttcgcttca ccggcagtaa tgccaatcag ctccacgccc
     2821 tggtaggacg ccaccacaat acacagagcg gtcaggaacc ctttccagcc acccgcaaag
     2881 aaaccgccat gctctgtgag attgctaaaa ccaatcgact gcccgccatt gccaaagcca
     2941 aagaaaatca cgcccaggcc aatgacaatc atcacgataa tcgtggtgac tttgatcatc
     3001 gcgaaccaga actcgatttc gccgtacaac cgcaccgccg ccagattcgc caacgccacc
     3061 agcgccaccg cgatcaatgc gggtatccac tgcgccatct ccggggacca aaactggaca
     3121 taaacgccaa tggcggtgat ttctgagatc cccaccgcca tccacataaa ccagtaagac
     3181 caggcggtga gatagccaaa aaacgggctc atataacgat gcgcataaac ggcgaacgaa
     3241 ccggtaaccg gttcgaggaa caacatttcg cccattgaac gcatgatgaa aaaaacgaac
     3301 agcccggcga tgatataggc caacaatacg gatggcccgg cccatttcag ggtactggcg
     3361 gcccccataa acaggccgac gccaatggtg ccccccaggg cgatgagttc gatatgtcga
     3421 gcttccagcc cacgctgtag ctctggtttg ttatctgcca taaatcctcg tgttgtgttt
     3481 gcatgctttc cggtgttacc ggttatcgtt atgggtacat cgagtgttgc gaatgttttc
     3541 gtaattcagg agaaatggca aataaagcat taaaaatttg aatgctttgt gtaataaaaa
     3601 agcagacagg cgacgaagtg accactccgt cgctttacaa agagaggaaa atcagaggtt
     3661 gccggtgtag tgccagcgta aataacgcag caaacgaagc tgacgcttaa tgcggctcgg
     3721 ctgcgaaagc aggcggtaga gccactccag tcccagcgtt tgccagattt tcggtgcgcg
     3781 ttttacgtga ccggtgaaaa catcgtaagt cccgccaacg cccatataca gcgcatctgg
     3841 atgcaccaga cggcagtcgc gcatgaagat ctcctgcttt ggcgatccca tcgcaacggt
     3901 gacgatttgc gctccgctgg catgaatgcg ttcaaacagc gcctgacgct gctcgggttt
     3961 aaaataacca tcctgactgc caacgatatt cacattccat tggttgcgca gtttagcttc
     4021 agtttgcgcc agcacttcag gtttaccgcc cacaagaaat accggcgtcc cttctttgcc
     4081 tgcgcgcgcc atcagctctt cccagagatc ggcaccggca acgcgggaaa cctgcgcctg
     4141 cgggtacttt ttacgtactg aacgtacaac gctgatgcca tccgcatatt taaattcggc
     4201 agcgttaatt aactccctga cctcggcgtt atcttcaatg gtcagcattt tttcggcatt
     4261 aatggcaacc agcgttccct gcttaagctg cccgtcagca aacagataat cgagggcatg
     4321 ctgcatatca cgccaaccaa tcaactgtaa gccacgcagc gtataggtcg gtgccgtggt
     4381 gttgttattc attgttatcc ttcaacctgc gtccggagcg atgattttgt acgtttatga
     4441 atgagtccgg cgctttcaaa aagccagtac aacagttttg cgatcatcag acatgcgccg
     4501 aagaccacga taaaaaagac tacgcgtgag acaaacgaat ccagcccttc acgtgccagc
     4561 acgatcatat tgaagatcgc cccaaagcag aaactgtgca atatcgcagc cttatagcga
     4621 ttagtctcgc ggttgcccag ctcatacagc cagtcgaacc atttgatgat cagtccaacc
     4681 acaatcgccc cgagtgggat gaacaacgcg ccgcccatca ccaccagtga gcctataagc
     4741 gtaggcgaga tcgccagtcc ggagtggtta ttcagtactt cccaggtaaa gtaattggct
     4801 gagttcagca ccatactcgg gcgccccggc cacagccagg aagggataaa gacatagaaa
     4861 tcgcggacaa ttggagccag gccctggaag tcgatgttgt cgtagttctg caacagcaac
     4921 gccagattct cccacggtga aaaggtgtcg cgagtgagat agagaaacgt atagaacgct
     4981 tcatcgccgc tcacattcat tccatagcgt tttaacgcca gccagaacat gccaacaatc
     5041 cccagcacgc ccgccgccgc cagcatccac aacgaaatcc agccgcgaat aatgccaata
     5101 aacaggaaga tagcgaatgc gatgatgata ttggcgcgag tgccgccgac aatcatataa
     5161 gtcagcaagc caaaggcgac cgtgctgacg aggaaaaaca gccacgcttt gctgtcctgg
     5221 cgcagaaagt agaccaccag catcgccggg atgaaaaagt aaaagaagcg ttttaacgcc
     5281 acgccggaga cttcactgga aaagatctga ctgtaggagt taagccggaa cagcaaaaag
     5341 ccgttgtgca tgaagaagat gccgacgctt accagcgcga tacccatcag gatcacccac
     5401 gtaagattgg tctccacgcg gttcatggta aacagcggac ggcgcggtac atcagcaacg
     5461 cgtttgcgta ggcgggtttt gtaggtgaca tagtaaaccg cgtagaagca gcccgcagaa
     5521 agcaacgcct gcaacaagat ttctggcggc gcgacaccaa catcaaagcg aaataccagc
     5581 acgctggtca gcgggaagcc gaagaaaaag gtgagcaaaa acagcaatga aaagaagaca
     5641 ttgaagttaa agcgcacacg gcgaaactca aaccaggtca gcgtggcaat aaacagcgtg
     5701 cagagcagcc agaccacaaa caggccactg aattgcagca gactcatgcg acctccccgg
     5761 cggcaatcgc caacgcccgc tgccagcctt gtagatagtt agggctaaag aaggcaatgg
     5821 tgtttttatc cgccgacgcc aactggcgct gcgcttcacg cacaatatcc tcgttgagat
     5881 cgtcggtagt aaacagcact ggcaaatgct gttccgtcat atcctgccag aacggatttt
     5941 ccctgttaag cacacaagga atgcccgcct gaatcagtaa gcaaagcgta ccaatgccct
     6001 gctggcgggc aaaaataaag taaccaagat cgcactgacg aagtagcgcc agataggcgt
     6061 caaattccag tttttcgctc aaaatttgca gattttcttc gctgaataac tccaaccccg
     6121 cctgacgaac ttcctcaatg tacgcttcgt tattaggcgg atatcccatc ggcaccacca
     6181 cttttaccgt atcgccaaat tgctgatgaa cggcacgtaa agcggcgaca tgctcattgc
     6241 tgcggtcgcc ggagttcccc accagaatgg tcatttttcc ttcacgttgc cgatcgttcg
     6301 ccatcgtatt gagagaaggg tccatccgcg tcgggaagta cagcagttct ccccgcacct
     6361 ttgggtgcgt tttggcaaaa aagctcaaat cgccgcgggt ggcaaataca cagccgactc
     6421 gcttttgcgc caggcgacgt agtgggtaaa aaagcttata tctcaagcca ctggaaagct
     6481 catacaggtc tgccccccag atatgccaga aaaactggct gggcttaatg ccaccactca
     6541 gcagagccag ccacagtgtg ggattgaact gaccgtggaa aaaaaatcgc tgctgacggt
     6601 tagcttttgc tttcgcgatg accgcttccg ccagcgattt tttcccaggg aaaaattgca
     6661 cagaaagcgc cggacagcta tcacttaagc cgtcgtcctt gccaacaacc ataaactcgc
     6721 acgcgtgctc gctcgtcgcg gccagcgcgt cattgaaaaa ccgcaaaacg gttcggttat
     6781 ggtgagggat atccgatccc agtacgtgaa tcagtacagt catgcccgcc tacgccagag
     6841 taaaaacacg ccacaacaaa gagaaaaata gacgatataa gttgccatat atgcctgcgc
     6901 cgcgcccagt gcaccatgcg cagggattag ccagtgggca aataccatca ataaagtgaa
     6961 ctggctgact tccgccagaa tataaaaccg cagtgacgct ttggcgatca ccagataacc
     7021 aaagacataa gcgcccactt ttaacacatc acccactaac tgccaggcaa agagatcgcg
     7081 cataacggta aatttattcg acaacagcaa ccagatagca aaatcacgca gcaaccagac
     7141 ggtaaaactc gctgccgcca ctgccggtaa gacgaatttc agcgatttaa ccacttcccg
     7201 ggtgatatcg cgcttttccg ttagccgcga caacgtgggc agcaaatata cgctgaacga
     7261 tgccgtaata aattgcaggt aggcatcgga aatactgctc accccttgcc agatccccac
     7321 ttcatcccag ctatactgcg ccgccagcag tttacgcatc atgatgtaag caacaggcaa
     7381 ggtcaccgac gtaatcaacg ccatgagcgt aaatttgctc aactgccctg ccagaccgtt
     7441 atcccagctg ggtttcagat agcttaacgg gatgacacca cgtttaatca acatgatggc
     7501 ggcaggaatt accaccagcg cgggaatcag cgccagaccc agcaacgccc cttcataacc
     7561 gccaaaacgg taactgacgt agtacgcgag aacgccaatc aagctgccga caatcagcga
     7621 taacgcatta cctgcggcat cgcgaaagcc tttcatcagc gccagtaaca ggttgcccca
     7681 ggcgatcccc atttgcacca gcgccactaa acgcaccagc ccctgatagt cggtattacc
     7741 aaacaatccc tggctgattg gcgcagctgc cagcacaaaa accagcgcca tcagcgtaga
     7801 gaaaccaagt accatcgctg atgaagtgcc gaccacgcgg cgcagctgtt gcggattatc
     7861 atggtactgg gcaacgtatt tggttacacc gttaaagatg ccagccccgg caagcacgcc
     7921 gagcacggta atcaactggc ggaaatttgc cgccagccca agccccgccg gaccaaatga
     7981 caccgccagc aacttaccga ccagtaaccc ggcaccaatc ttgaccagtg tactggccgc
     8041 cgtccacaag gacgcttttg ccaacgacat atcaggagaa gtagttcaac aaagtcgcaa
     8101 ttaccgtacg ctgattgacg ggcgacaggt tgtagaacag cggcaggcgc agcaggcgct
     8161 cgctctcttt ggtggtgtag cgatcttcac cgtggaactc accaaagtgt tcccccgcag
     8221 ggcaaccgtg cagcggaatg taatgaaaca ccgccatgat ttccgcttct ttcagaaagt
     8281 taatcaacgc gctccggtca tcaatatccc gcagtttaat gtagaacata tgcgcgttct
     8341 gcacgcagcc atcgggaatc gacggcaact cgatacgccc ggctttcgcc agaggcgcta
     8401 acgtatcgta gtagttttgc cacagcgcca gacgttgctg gttgatacga tccgctgctt
     8461 ccagttgcgc ccacaggtac gcagcttgca gatcggacat caaatagctg gagccaatat
     8521 cgcgccaggt atatttatcg acctgaccac ggaagaactg gctgcggtta gtgccctttt
     8581 cacggatgat ctcggctcgt tcgattaacg ctttatcgtt aatcagcgtc gcgccgcctt
     8641 caccacccgc cgtgtagttt ttggtttcat ggaagctaaa gcagccaata tgaccaatgg
     8701 ttcccagtgc acgccctttg taagtggaca tcacgccctg agcggcatct tccaccacaa
     8761 acaaattatg ctttttcgcc aacgccataa tggtgtccat ttcgcaggcc acacccgcgt
     8821 aatggaccgg cacgataacg cgcgttttgt cggtgatcgc tgcttcaatc aacgtttcgt
     8881 cgatgttcat ggtgtccggg cgaacatcca caaagacgat tttcgcgcca cgcagcacaa
     8941 aggcattggc ggtggagaca aaggtgtagc tcggcatgat cacttcatcg ccaggctgga
     9001 tatcgagcag cagcgccgcc atctccagcg aagcggtgca ggacggcgtc agtaacactt
     9061 tggcgctgcc aaaacgttgc tccagccact gctggcagcg acgggtaaaa ccgccatcgc
     9121 cacacagttt gccgctaccc attgccgact gcatatagtc gagttcggtt cccaccaccg
     9181 gcggtgcgtt aaatggaatc atgtgatcac ctgtataacc agtacgcggt gctttctaca
     9241 ttcgcaccac tttgtatgta tcgtttaagc gcggcggtgt tgcccatttg ggtcgccacc
     9301 cgcaaagttg ttttaccgcg agcatacgcc cagtttagcg ccgtttgcat cagctcagca
     9361 cctgcaccgc gtccagccag caggccaatt cgcgcatctg tcgcattgag ttcccgtaaa
     9421 gagacatagc cgcgaatatc gccggacgcc gcacgtagaa tcagacattg atgatcaaag
     9481 gtgccgcgca cggcattttc aatccactgt gcataaaagc gaccgctggc gtcaggcgca
     9541 taccacggcg cacgaaaacg gctttgcgca aatgcggcgc tagctaactg acgtaatgcg
     9601 ggaatatcgg tctcttgtgc cacgacagca ccgctatcac tgacattgtt cacgggtagc
     9661 gccaaatcaa cttcaccttc taccagggag aatcccagct gttgcagggc atccagttcc
     9721 cccgtatttg atgccgcaat tttggcctgc acccgtgacc atggcgctaa ggcgtctgac
     9781 gtcaggagcg gtgcttcaga cgtaatgcgc acgatggcgc tgttaacacc aaagaaggcg
     9841 ttttcccagg ttagtggctc aatactggcg cggacgggca cgaagtaact ccagcagata
     9901 ttggccgtag ccagttttcg ctaatgaact ggcagcacgc ttcacaccct cgtcatcgag
     9961 ccagccgtta cgccaggcga tctcttccag gcaggcaatc ttaaagccct ggcgtttttc
    10021 tactgtctgc acaaaagtgc tggcctcaat caggctgtcg tgagtgccgg tatccagcca
    10081 ggcaaatccg cgcccgagca gttcaacggt caggttgccc gcctcgaggt acatctggtt
    10141 gatggaggta atctccagtt caccgcgctc cgacggcttc acctgctttg cgtactccac
    10201 gactttactg tcgtagaaat aaagcccggt caccgcccag tttgacttcg gctgttttgg
    10261 cttttcttca agcgagatag cgcggaaatt gtcgtcaaat tccaccacgc caaagcgttc
    10321 cgggtccatc acctgatagt caaaaaccgt cgccccttcc gtgcgcgccg caacatgacg
    10381 cagcttcgga ctgaagccct gaccgaagaa gatgttatcg cccagcacca gacaagaagg
    10441 ttcaccatta aggaaggttt caccgatgat aaaggcctgc gccagaccgt ccgggctggg
    10501 ctgttcggca tattccagct ggataccgaa ctcactacca tcgcccagca ggcgctggaa
    10561 ataaccttta tcttccggcg tagtgatgat gagaatttcg cggataccgg ccagcatcag
    10621 caccgacagc ggatagtaaa tcattggctt atcgtaaatc ggcaacagtt gcttcgatac
    10681 gccgcgcgta atcggatgca atcgggtacc ggaaccgccc gccaggataa tacccttcat
    10741 gtttgcctcc gctgaaaatt agcctttcag acctaaacgc tcaccctgat agctgccgtc
    10801 ctgcacctgc ttccaccagc tttcattagc cagataccac tgaaccgttt tacgcattcc
    10861 actttcaaag gtttcctgcg gcagccagcc aagttcacgg gcaattttcg aagcatcaat
    10921 ggcataacgc agatcatgcc ccggacggtc agcgacaaag gtgatcaggt cacgatagtg
    10981 cgccacgccg tgcggcttgt tcgaagccag ttcttccagc agctcgcaaa tggtttccac
    11041 aacatcgaga ttcttacgct cgttgtgacc accaatatta taggtttcac cgactttccc
    11101 ggtggtcgcc acgcaataca gcgcgcgggc gtgatcttcc acatacagcc agtcacggat
    11161 ttgctgcccg ttgccatata ccggcagcgg tttacccgcc agcgcgttga ggatcatcag
    11221 cgggatcagt ttttccggaa agtggtaagg accgtagtta ttcgagcagt tggtgatcag
    11281 cgtcggcaga ccgtaggtcc gcagccaggc gcgcaccaga tggtcgctgc tggcttttga
    11341 cgcggaataa gggctgctcg gcgcatacgg cgtggtttcg gtgaagaaat catccgtcga
    11401 gtgcaggtca ccatatactt cgtcggtgga gatatgatga aaacggaacg ctgatttttt
    11461 atcttccgtc agcgtattcc agtaagcccg cgccgcttca agcaatgtat aagtcccgac
    11521 aatgttggtt tcaataaacg ctgccgggcc gtcaatagaa cggtcaacat ggctttcggc
    11581 tgccagatgc atgacacagt ctggctgatg ctcagtgaat acgcgtgcca gttctgcccg
    11641 atcgcagata tcaactttct caaaggcaaa gcgctcgctt tgcgcgaccg gtgccagcga
    11701 catcaggttt ccggcgtagg tcagcttatc gaccactacc accgcatcgc tcgtttcgtt
    11761 gatgatataa cgcaccagcg ccgagccaat aaacccggca ccacctgtta tcagaatttt
    11821 tctcatcagc gccagactcc tttggcatcg acgacatact gctgatggac attgtcgcca
    11881 ttgataactt tgaactgact atgatcgacc agcatcacca gcacgtctgc cgttgccagc
    11941 gcctcgtcaa gctgcgccag agtacaaagc ccggtcagtt ttttcggcaa ctggtggatg
    12001 ttaggctcaa caaccagcgt ttcgccgctg tgccactggg cgatcagttc agcgatttcc
    12061 atcgccgggc tttcgcgcag gtcatcaata ttcggtttaa acgccagacc aaagcaggcg
    12121 attttcagtt cactggcgcg tttatcggta gcagcaaggc agtcggcaac cgccgctttc
    12181 acctgatcga taacccagaa cggtttgtga tcgttaactt cgcgcgcggt acggataagc
    12241 cgcgcctgct gggggttctg tgccacgata aaccacggat caacagcaat gcagtgaccg
    12301 cccacgccag ggccaggctg aagaatatta acgcgagggt gacgattcgc caggcgaatc
    12361 agttcccaga cgttaatccc ctgatcggca caaatcagcg acaattcatt agcaaaagcg
    12421 atattcacat cgcggaagct gttttcggtg agcttacaca tttccgccgt ccgcgagtta
    12481 gtgacgacac actcaccttc gaggaaaatt ttgtacaatt cgctggcgcg ggccgaacaa
    12541 accggcgtca taccaccaat cacgcgatcg tttttaatca gctcgaccat aacctggccc
    12601 ggtaatacac gttccgggca gtaagcaatg ttgacgtccg cctgctcgcc cacctgctgt
    12661 gggaaagtca gatccgggcg catctctgct aaccattcag ccatcttctc ggttgacccc
    12721 accggcgagg tggattcaag gatcaccagc gcgccttttt tcagcactgg cgcaatggag
    12781 cgagcagccg attcaacgta ggtcatatct ggctcatgat cgcctttaaa cggcgtaggt
    12841 acagcaatca gccaggcatc cgcttcaact ggcgtcgtgc tcgctcgtaa aaaaccgcct
    12901 tctacggcag tttttactac gctcgccaaa tcaggctcga cgatatggat ttcgccacga
    12961 ttgatggtat caaccgcgtg ttggttgata tcgacaccaa tcacctgttt ttgccgtgag
    13021 gcaaacgctg ctgctgtagg cagcccgatg taacccagtc cgataacaga aatggtcgca
    13081 aaactcatag tgatatccga ttatttttta acgcttccag aatgcgagag catgcctgac
    13141 catcaccata cgggttatgg gcgcggctca tagcttgata ttcgttttcg tcttttaaaa
    13201 gacgcgtcac ttcctcgaca attcgctgct tatccgtgcc taccagacgc accgtacccg
    13261 ccgtcaccgc ttccggacgc tcagtggtat cgcgcatcac cagcaccggt ttccccagcg
    13321 aaggcgcttc ttcctgaatg ccgcctgagt cagtcaaaat cagccaggcg tggttcatca
    13381 gccagacaaa cggtaaatac tcctggggat cgatcagaat gacatttttc acatgcccca
    13441 gaatgcgatt gaccggctct ctgacgttcg ggttgagatg caccggatag acaatctgga
    13501 tgtcctggtg cgtggtggcg atgtctgcca gcgcgtggca gatttcttca aagccacgac
    13561 cgaaactctc acgcctatga ccggtcacca gaatcatctt tttatcgggg gcgataaacg
    13621 ggtaatttac cgccagttct gaacgcagcg tgtcgctgct catcacctgg tcacgcaccc
    13681 ataacagtgc atcaatgact gtattaccgg taatgaagat tcggctatcc gcaacgtttt
    13741 cacgcagcaa gttttgccgg gaagtttcgg ttggagagaa gtgatacatc gccagatgcc
    13801 cggtcaatgt acggttagcc tcttccggcc acggcgaata gagatcgccc gtgcgcagac
    13861 cagcctcaac gtgaccaaca ggaatacgct gataaaacgc cgccaggctg gttgccagcg
    13921 tcgtcgtcgt atcgccgtga accagcacga cgtctggttt gaactcggca agaataggtt
    13981 ttagcccttc cagaatccga caggttatct ctgtcaggcc ctgtcctggc tgcattatgt
    14041 tgagatcgta gtcaggtaca atggaaaaga gtttcagtac ctgatcaagc atctctcgat
    14101 gctgcgcagt gacgcaaact ttagcctcaa aaaaaggatc ttttgccaac gcatgcacca
    14161 acggagccat cttgatggct tccgggcgcg taccaaatac agtcagtact ttcacatcga
    14221 ttctcttcga ataagcggcg agcgcctttg cgctcaccgc agcagtgttg ctatttcgag
    14281 caacggcggg ttaatgcgac accagccccg atcagccccc cgacaatgcc ccacataatc
    14341 atcaggaagg cacgacgtgg gctatcgcgt tttaccggtt cttccggcgt acgcaaatag
    14401 cgataggtct gaaaacgcgg atccagggtt ggaccaacat tcagggtgtt taacatggct
    14461 cgattctgat catagtcgag atcaaaggcc ggaccgacgg cctgtaaatt ttccagtcga
    14521 gcctggagca ttggacgccc aagcaggaac atttctgaat caggtaattc ctcggcaggc
    14581 acatctgtcg cactgcgcga aatattatgc tgctcagcaa ttttcagcgc ctgctcaatg
    14641 ctgttcatcc ggcggtcgta gatggctttc gccacctctt cctgacgctt cacctgagct
    14701 ttcatctgga tggtgcgtgc cgcccatgcg cctttcagct catcattcag atggctggct
    14761 gcacgctggc tggcaaaagc aacatactga cgtaacaggt tattagcgtc aggcgcggtt
    14821 tcggcaataa gcttcacgct gtcattgacc gcgcgagtaa agtctccggg gataaactgg
    14881 atgttgttaa tcatttcatc cagcaacgcc gcatcggctt tgctgttgcc caccatccgc
    14941 tgtttgtaat agtcggtttg cagccagaac tctctgcggg tatcccacga ggccagttgc
    15001 ataacaaact ctttataggc ttcgtccatg accgatggtt gatcggcaga agccatgttt
    15061 gaacggacat ccaggttacg caaaaattgc tgctgcgagt aatatccccc cagcatattc
    15121 accgttggac gatcggtaat cgccgtcgag ctccactcct gacgagcaaa aaaagtatac
    15181 gccagcgcga ttaacgcaaa cgccagcccc atgccaataa tccatagctt cccagcccac
    15241 aaggtacgaa acaacccacg aatatccagt tcattttcag cgtcttcggc cggtttccca
    15301 ggcattggtt gtgtcatcac atcctcattt atttggttaa attggggctg ccaccacgat
    15361 ttctacgcag tctgcgtttt acgcgcttaa taaagcgagc aactttccag gcacgcttaa
    15421 tgcaatatcc atagaggaag aatgctagca aaaagagcac cagcatgacc cactccggga
    15481 caaaatgaga atattctgcc agcacgccaa tggaagcgag cagtgctgcg gcaagggtaa
    15541 tcagcacaaa cgcctgacgg gaagtaaacc cggcacgcat gatcaaatgg tgaatatgct
    15601 gacggtcagg agagaatggg ctcatgcctt tacgcaggcg acggtacata atcgccacca
    15661 tatccattag cggaatggcg attatccaca aagcggtaac cgggctgatg ggatgggttt
    15721 tgccctgggt cgtttcgagc aggatccaga taacggtaaa accaatcagc gtactgcccg
    15781 catcacccat aaagactttg tagcggcgac ccaggatacc aaggttaagc atgatgtatg
    15841 gcaggatggc ggcgatcatc gcaaagcacc agattgcgag gctggtttgc ccgtcgaacc
    15901 acaaaatcat accgattgct gcaaacgaga cgcaggacaa cccgcccagc aagccatcaa
    15961 tgccatcaac catgttgaac gcattaatgg ccgcccagac ggcaaatagc gtcaggaagt
    16021 aaccaaacgg tccgagcacc atctcccagg agccaaagat ataacccagg ctactgagat
    16081 aaagtttgcc gaacaccatc ataacaatgc caacagcggc ctgtatggtg gcacggattt
    16141 ttacgctgat atcaaaacga tcatccagcg cgccaatgaa aacaagcaca ccggcacaag
    16201 cgagatagag agatgcatgc ggaatatagt aatcgacaat tccgaacgtg aagcaaatcc
    16261 ctgcgtaaac cgaaatcccc ccaacgagag gtatcaatcc ctggtgacgt ttgcggaagt
    16321 ttggtttatc cactaaaccg actttttttg ccaccttacg ggcaaaaaac agaaacagtg
    16381 tcgtgaataa aaaaatactg atgagatcag tactcactgt cagtaaattc acaatgcatg
    16441 ctctcagaga aaattattag cagaagtata accacgaaga cctttattca gaagggaaag
    16501 tccgttccga acccaccagt cctgtgcaaa aattaaccat ttgatatgca aattgcataa
    16561 catgccagca aattccagga attttcctaa catcgccagc gcgacattaa gattacagat
    16621 ataaaagcaa aacgccacgt aaacacgtgg cgtttttggc ataagacaaa tttatgagcg
    16681 tttcatcatt tcgaagaaat cgtcattggt cttggtcatg gccagtttat taatgaggaa
    16741 ttccattgca tcgatttcgc ccatcgggtg aatgattttg cgcaggatcc acattttctg
    16801 cagttcttcc tgagtcgtga gcagctcttc tttacgggta ccggaacggt tgtagtcgat
    16861 agccgggaag acgcgttttt cagcgatctt acgagagagg tgcagttcca tgttgcctgt
    16921 acctttaaac tcttcgtaaa taacttcgtc cattttagaa ccggtatcga taagcgccgt
    16981 cgcgataatg gtcaggctgc cgccctcttc cacgttacgc gccgcaccaa agaagcgttt
    17041 cggacgatga agggcattgg cgtccacacc accggtcaac actttacctg acgccggaac
    17101 aacagtgttg taagcgcgcg ccagacgagt gatggagtcg agcagaatga taacgtcttt
    17161 cttgtgctca accaggcgtt tggccttctc gatcaccatt tccgcaacct gaacgtggcg
    17221 agatgcgggt tcgtcaaagg tagaagcaac aacttcacct tttaccagac gctgcatctc
    17281 ggttacttct tccggacgtt cgtcgatcag cagaaccatc agcacacaat ccgggtggtt
    17341 gtaagcaatg ctctgagcaa tgttctgcag cagcatagtt ttaccggctt tcggcggtgc
    17401 cacaatcaga ccacgctgac cacgaccgat aggtgatgcc agatccagta cgcgagcagt
    17461 taaatcttca gtagaaccgt taccacgttc catacgcaga cgagagtttg cgtgcagcgg
    17521 ggttaagttc tcaaagagga ttttgttgcg ggcgttttca ggtttgtcga agttaacttc
    17581 gttaactttc agcagcgcaa aatagcgttc accttctttc ggcgggcgaa tcttaccaga
    17641 gatggtatca ccagtgcgga ggttgaaacg gcggatttgg ctaggggaaa cgtagatgtc
    17701 atcaggaccg gcgaggtagg agctgtctgc ggaacggagg aaaccaaatc catcctgcaa
    17761 tatctccagt acgccatcac caaagatatc ttcgccactc tttgcgtgct gcttcaggat
    17821 ggcaaaaata atgtcctgct tacgcatgcg agccaggttt tccagcccca tattttcgcc
    17881 gagagtgatc agctcagaaa ccggcgtatt ctttaattcg gtaagattca tagtggtgtg
    17941 agttcttaaa cttggggtaa atctcgaact taatgttgtg aatggtatgg caggatcatc
    18001 catgcctgtt tacggtcatc gactcatgtc ttttcgctgc ctggtcacag gagagtacgc
    18061 agaactgaaa cgacaagacg gattgagtga cgagccagaa atctgttcac ttcgcattta
    18121 agataaaacg ggaagcgttg gaaattacaa gattcaaact taataaggta tgtttaatac
    18181 gaagtcaaca ctaagttagc atgactcacg ccgggcgtcc agtttttagc gacggggcac
    18241 ccgaacatga aattccctta cgccaggtta gcgtcgagga actctttcaa ctgaccttta
    18301 gacagtgcgc ccactttggt tgccgccact tcaccgtttt tgaacagcag cagagtcggg
    18361 ataccacgga tgccatattt cggcgcagtg ccagggtttt gatcgatgtt cagttttgca
    18421 acggtcagtt tgccctgata ttcgtcagcg atttcatcca gaatcggggc gatcattttg
    18481 cacggaccgc accactctgc ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca
    18541 tccgtgtcaa aactgtcgtc agtcaggtga ataattttat cgctcatata taactccaca
    18601 ggaataagcc tggcgtgttg gtttcgttgt tggtgtaaca ttaaccaact aaaggttgac
    18661 tttatttcac cggatacgct ttcgtaaagc aatagtaagc tgatattcta ccacactatg
    18721 agcaaaacac atttaacaga acagaagttt tccgacttcg ccctgcatcc gaaggttgta
    18781 gaagcccttg aaaaaaaagg gtttcataac tgtacgccca ttcaggcact ggcccttccg
    18841 ctgacgctgg cgggtcgtga cgtagccggg caggcgcaaa ccggtaccgg gaaaacgatg
    18901 gcgtttctta cgtcaacgtt tcattatctt ctctctcatc ctgcgattgc cgatcgcaag
    18961 gtgaatcaac cgcgtgcctt aattatggca ccgacgcgtg aacttgccgt gcagatccac
    19021 gccgacgcgg aaccgctggc ggaagctacc ggcctgaagc tgggcctggc ttacggtggt
    19081 gatggctatg acaaacagct gaaagtgctg gaaagcggcg ttgacattct gattggcacc
    19141 acgggtcgtt taattgacta cgccaagcag aaccacatta acctcggtgc cattcaggta
    19201 gtggtactgg acgaagccga tcgcatgtac gatctaggct ttattaaaga tatccgctgg
    19261 ctgttccgcc gtatgccgcc tgcaaatcag cgcctcaaca tgctgttctc cgccacgctt
    19321 tcgtaccggg tacgtgaact ggcgttcgag cagatgaaca atgccgaata tattgaagtg
    19381 gaaccggaac agaaaacggg ccaccgtatt aaagaagagc ttttctaccc ttctaacgaa
    19441 gaaaaaatgc gtttgctgca aacgctgatc gaagaagagt ggccagacag agcgattatt
    19501 ttcgccaata ccaaacaccg ttgtgaagag atctggggcc acctggcggc agatggtcat
    19561 cgtgtcggtt tgttgacagg cgatgtcgcg cagaaaaaac gtctgcgtat tcttgatgaa
    19621 tttacccgtg gcgatctgga tattctggtt gccaccgacg ttgccgcgcg tggtttgcat
    19681 attccggcag tgacgcacgt ctttaactac gatttacccg atgactgtga agattacgtt
    19741 caccgtattg gtcgtacagg tcgcgcaggc gcaagcggtc actctatcag cctggcgtgt
    19801 gaagagtatg cattgaattt gcctgctatt gagacctata ttggtcactc aattccggta
    19861 agcaaataca atccggacgc attgatgacc gatctgccaa aaccgctgcg cctcacgcgc
    19921 ccgcgcacag gcaatggtcc gcgtcgtact ggcgctccgc gtaatcgtcg tcgttcaggt
    19981 taaataaaat gtcatactca aaatcattca aactgtatca agatggcaag agaatgaatc
    20041 ccgatgagct tactgtagta agtgattcgg gtgaaagaat gcagccaaca cagagacaga
    20101 ttgaaggatg aagagtatgg gttccacctc gtcgctgtat gcagccattg atctcggttc
    20161 gaatagtttt catatgctgg ttgtgcgcga ggtggctgga agcatccaga cgctgacgcg
    20221 aattaaacgc aaagtgcgtc tggctgctgg cctgaatagc gaaaatgacc tctctaatga
    20281 agcaatggag cgcggttggc aatgtctgcg cctgtttgct gaacgtctgc aagatatccc
    20341 tccctcgcaa attcgcgttg tcgctacggc gacattacga cttgccgtca atgcgggtga
    20401 ttttattgcc aaagcgcagg aaatcctcgg ttgtccggta caggtgatca gcggtgaaga
    20461 ggaagcgcgt ctgatttatc agggcgttgc tcacaccact ggtggtgccg atcagcgcct
    20521 ggtggtggat ataggcggtg ccagtactga actggtaacc ggcacgggtg cacaaaccac
    20581 ctcgttgttc agcctgtcga tgggctgcgt cacctggctg gaacgctatt ttgccgatcg
    20641 taatctgggg caagaaaatt ttgatgctgc agaaaaagcg gcacgcgaag tgttacgtcc
    20701 ggttgccgat gaattacggt atcacggctg gaaagtgtgc gttggtgctt ccggcaccgt
    20761 gcaggcgtta caggaaatca tgatggcaca ggggatggat gaacgcatta ccctggaaaa
    20821 gttgcagcaa ttgaaacagc gagccattca ttgcggtcgg ctggaggaac tggagattga
    20881 cgggctgacg ctggaacgtg cgttagtgtt cccgagtggt ctggcgatcc tgatcgccat
    20941 ttttaccgaa ctgaatattc agtgtatgac cctggcgggc ggtgcgctgc gtgaaggcct
    21001 ggtctacggc atgttgcatc tggccgtcga gcaggatatt cgcagccgta cactgcgtaa
    21061 tattcaacgc cgctttatga tcgatattga tcaggcacag cgcgtagcca aagttgcggc
    21121 taacttcttc gatcaggtgg aaaacgaatg gcatcttgaa gcaataagcc gcgatttgct
    21181 catcagcgcc tgtcagcttc atgaaatcgg cctgagcgtt gacttcaaac aagctccgca
    21241 acacgctgct tatctggtgc gtaacctgga tcttcccggt tttacccccg cacagaagaa
    21301 actgctggcg acgctactgc tcaaccagac taacccggtc gatctctcat cgctgcatca
    21361 gcaaaatgcc gtaccaccgc gcgtcgcaga acaactctgc cgtttactcc gcctggctat
    21421 catttttgcc agccgtcgcc gtgacgatct cgtgccagag atgacattac aggctaacca
    21481 tgaactgttg accttgacgc ttccgcaagg ttggctaacc caacatccgc tgggtaaaga
    21541 gattattgct caggaaaacc agtggcagag ctatgtccac tggccgctgg aagtgcatta
    21601 atgagtaagt gccggatgcg atgctgacgc atcttctccg gccttgatta tttccctcgt
    21661 tttaccgcca tcatcgcttt cagattcgcc agatggcttt gccctttctg catccgttct
    21721 tcggcactga ccactttgcg ctcctgttcc caaatcagat catcctgcgg cagctccagc
    21781 aaaaagcggc tcggctccgg gcgcaccagt tcgccgtact gacggcgttc tttacacagc
    21841 gtaaaggtca attccttctg ggcgcgggta atgccgacat aggccagccg ccgctcctca
    21901 tcgatattat cttcatcgat actgctctgg tgcggcaaaa acccttcttc cataccgacc
    21961 atgtagacat aaggaaactc cagccctttt gacgcatgga gagtcatcag ttgcacctga
    22021 tccagctctt cttcactctc accacgctcc atcatgtcgc gtaaagtaaa gcgcgtcacc
    22081 acctgggtga gcgtcatcgg ctcatccagt tcgctgcctt ccagcatctc cgtcatccag
    22141 ctaaacagtt ggttgacgtt cttcatgcgc atttcggcag ctttcgggct gggcgatgtt
    22201 tcgtacagcc aggattcata atccatgccg tggatcagat cgcgcaccgc ggcaatcggc
    22261 tcccgctccg ccagacgctg gatttctgcc aaccagtgag tgaagcgggt caatgcttca
    22321 taaccacgtc cgctaagcgt ctgactcagg cccatatcaa agctggcggt aaacatgctt
    22381 ttattgcgcg tcatcgccca ctcacccagc tttttcagcg tcgccgggcc aatctctcgc
    22441 ttcggcgtgt taacgatacg cagaaatgcg ctgtcatcgt ccgggttagt cagcacgcgc
    22501 agataagcca gcaagtcctt gatttcagga cgagagaaaa acgacgtacc accagaaatt
    22561 ttgtacggga tgcggttttg catcaggaat ttttcaaaca cccgcgactg atggttaccg
    22621 cgataaagaa tagcgtaatc tttgtactgc gttttattga cgaagtgatg cgcaatcagt
    22681 tcaccagtga cgcgctcggc ttcatgttct tcgttattcg cgcttaaaac ttttagctct
    22741 gtgccgtaac ccagttcgga gaacagacgc ttttcaaaga cgtgcgggtt attggcgatc
    22801 aggatgttcg ccgctttcaa aatacgtccg gaagagcggt agttctgctc aagcttaatc
    22861 accttcagcg ccggaaaatc ctggctcagc agcaccaggt tttgcggacg tgcgccgcgc
    22921 caggagtaga tcgactggtc atcgtcaccc accacggtaa agcgcgcgcg gctgcccacc
    22981 agcagtttca ccagttcata ctggctggtg ttggtgtcct gatattcgtc caccagcagg
    23041 tagcgaattt tgttctgcca gcgctcgcgg acttcttcat tgcgttgcag aagtagcgtc
    23101 ggcaataaaa tcagatcatc gaagtcgagc acattacagg ctttcaggtg tgcatcatac
    23161 agcccataac aatgggcaaa aatacggtcc cgctcgccaa tcgcactggc tgccgcctgg
    23221 gacggtgttt tgagatcgtt cttccagtta gagatggtcg aaatcaactg ttgcaggaga
    23281 actttgtcat cttcaatcag cccttcggtc aactctttga gcaacgccag ctgatcggta
    23341 tcgtcaaaca gcgaaaagtt cgccttcatc cccagcgccg catactcacg cttgatgata
    23401 tccagcccca gcgtgtggaa ggttgagatc atcagcccac gcgcctcttt gcgacccagc
    23461 gtctgtccga cacgctcttt catctcgcgc gccgccttat tggtaaaggt caccgccgcg
    23521 atatgtcgcg cctgataacc gcacccgcgg atcagatggg cgattttatt ggtgataaca
    23581 cgagttttac cggaacccgc acccgccagc accaggcagg ggccggtaac gaattcgaca
    23641 gcttgttgtt ggccggggtt tagacgcata ggaaaatcgc tcaatgaaag tcgggagggg
    23701 aaaataacag cgtggtagta tagccagcct catccttacc actcaaggca cgatcatggc
    23761 aaaaacagca gcagcactgc atatccttgt aaaagaagag aaactggctc tggatcttct
    23821 cgtgcagatt aagaacggtg ccgatttcgg caagctggcg aagaaacact ccatttgccc
    23881 atcaggcaaa cgcggcggtg atttaggtga attccgccag ggtcagatgg ttccggcgtt
    23941 cgataaagtt gtgttctctt gtccggtact ggagccaacc ggcccgctgc acacccagtt
    24001 cggttaccac atcattaagg tgctgtaccg taactaatag caaggccttc tccgggagaa
    24061 ggccttgaac tctgaatcgc tattaacccg caacagcaat acgtttcata tccgtcatat
    24121 agccgcgcag tttcttacct acttgctcaa tcgcatggct gcgaatcgct tcatttacat
    24181 cacgcagctg cgcgttatct accgcacctt ccggaatagc tttacccaaa tcgcccggtt
    24241 gcagttctgc cataaacggt ttcagcagcg gcacacaagc gtaagagaac agatagttac
    24301 cgtactcggc agtatcagag ataaccacgt tcatttcata cagacgctta cgggcgatgg
    24361 tgttggcaat cagcggcagt tcgtgcagtg attcatagta agcagattct tcgatgatgc
    24421 cggaatcaac catggtttcg aacgccagtt caacgcccgc tttcaccatc gcgatcatca
    24481 gtacgccttt atcgaagtac tcctgctcgc cgattttgcc ttcatactgc ggcgcggttt
    24541 cgaaggcggt tttgccggtc tcttcacgcc aggtcagcag tttcttatca tcgttggccc
    24601 agtccgccat cataccggag gagaattctc cggagatgat gtcgtccata tgtttctgga
    24661 acagcggtgc catgatctct ttcagctgtt cagacaacgc gtaagcacgc agtttcgccg
    24721 ggttagagag acggtccatc atcagtgtga taccgccctg cttcagcgct tcggtgatgg
    24781 tttcccaacc gaactgaatt agtttttctg cgtatgccgg atcggtgccc tcttccacca
    24841 gcttgtcgaa gcacagcagc gagccagcct gcaacatacc gcacaggatg gtttgctcac
    24901 ccatcaggtc agatttcact tccgcaacga aggacgattc cagcacgccc gcacggtgac
    24961 caccggttgc tgccgcccat gctttagcga tcgccatgcc ttcgcctttc ggatcgtttt
    25021 ccgggtgaac ggcaatcagc gtcggtacgc cgaatccacg tttgtactct tcacgcactt
    25081 cggtgccagg gcatttcggc gcaaccatga ctacggtgat gtctttacgg atctgctcgc
    25141 ccacttcgac gatgttgaaa ccgtgcgagt aacccagcgc cgcgccgtct ttcatcagtg
    25201 gctgtacggt gcgcactaca tcagagtgct gcttgtccgg cgtcaggtta accaccagat
    25261 ccgcctgtgg gatcagttct tcgtaagtac ccactttaaa accattttcg gtcgctttac
    25321 gccaggacgc gcgcttctcg gcaatcgctt ctttacgcag agcgtaggag atatcgagac
    25381 cagaatcacg catgttcaga ccctggttca gaccttgtgc gccacagccg acgatgacta
    25441 cttttttacc ctgaaggtag ctcgcgccat cggcgaattc atcgcgcccc ataaagcgac
    25501 atttgcccag ctgtgccagc tgctgacgca gattcagtgt attgaagtag ttagccatgg
    25561 tgattcctcg tggtgttgtg cttcttattg ttcggttcgc gtttgcgaga ttgaattcac
    25621 tatatgacag gaaatttatt gcggaaattg atatattcac aacgtcacat tgcaatttct
    25681 gcaacgtcaa cattgagggc tgcgccattg gatttacgcg atctgaaaac cttcctgcat
    25741 ctggcggaaa gccgccattt tggccgcagc gcgcgggcga tgcacgttag cccgtccacg
    25801 ctctcacggc agattcagcg cctggaagaa gatctcggcc agccgctgtt tgtgcgcgat
    25861 aaccgcacgg tgacgctgac tgaagcgggc gaagaactgc gcgttttcgc ccagcaaacg
    25921 ctgttgcagt atcagcagct gcgccacacc atcgaccagc aagggccgtt gctctatggc
    25981 gaattacata tcttctgctc ggtgaccgcc gcttacagcc atctgccgcc gattctggat
    26041 cgcttccgcg cggagcaccc gtcggtggag atcaaactca ctaccggtga tgcggcagat
    26101 gcaatggaaa aagtcgtcac aggtgaagct gatctggcta ttgccgggaa gccagagact
    26161 ctgcccggcg cggtggcgtt ttcgatgctg gagaatctgg cagtggtgtt gattgcaccc
    26221 gcattgccct gcccggtgcg taatcaggtt tctgtcgaaa agccggactg gtcaacggta
    26281 ccgtttatca tggccgatca ggggccggtg cgccgccgca ttgagttgtg gttccgacgc
    26341 aataaaatca gtaacccgat gatttacgcc acggttggcg ggcatgaagc gatggtatcg
    26401 atggtggcgc tcggctgtgg cgtggcattg ttgccggaag tggtgctgga aaacagcccc
    26461 gaaccggtgc gtaaccgcgt gatgatttta gagcgcagcg atgagaaaac gccgtttgag
    26521 ctgggcgtgt gtgcgcagaa aaagcggcta catgagccgc taattgaggc attctggaag
    26581 attttgccga accattaacc cgccaaaaag aacctgaacg ccgggttatt ggtttcgtcg
    26641 tggcaatcgt agcccagctc attcagccgg gtttcgaaat ccggttcatg gtcgccaagt
    26701 tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg gaacaaagaa
    26761 atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg tgattccggg
    26821 aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc gaccatatag
    26881 cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta gccgccgtcg
    26941 ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg cacgccgaca
    27001 aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac cgatcgcccg
    27061 ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt caccgccaac
    27121 aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag gccgtggaag
    27181 ttcacgttgg caccggaaag aatatgcgcc agccgttcgc cgcgaatgtt gtgctgggcg
    27241 atatattttt tcattcccgc cagcgccagc gcgccagagg gttccgccac cgcacgcaca
    27301 tcttcgaata aatccttcat cgccgcacag atcgcatcgc tatcgacggt gatgatgtcg
    27361 tcgagatact cctggcataa acggaaggtt tcgtcaccga tgcgttttac cgcgacacct
    27421 tcggcgaaca gtcccacgcg tggcagatca accggatgac ccgcatccag cgctgctttc
    27481 aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgcggcat cagttgtttg
    27541 atcagcaccg ctacgcccgc cgccagaccg ccgccgccga ctggcacaaa tacgcggtcg
    27601 agatgggagt cctgctggag cagttccagc gccagcgtgc cttgcccggc aatcaccatc
    27661 ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc gatcgcttta
    27721 gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa tccacgtacc
    27781 gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt cacgcctaac
    27841 cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt gatcacgccg
    27901 tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg cagcttaaag
    27961 ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag acgcgacgac
    28021 agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg cgcgcgcagc
    28081 actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga gtcagccatt
    28141 attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg gttgccaggc
    28201 tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc gtccaggctt
    28261 tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg cttacctgta
    28321 actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca atcagaccaa
    28381 tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca gaggtgccgc
    28441 cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt gatttcagga
    28501 agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct tcatagcgga
    28561 tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc gcgtcgtcct
    28621 ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg acgcccgcgg
    28681 ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg ccgccgtctt
    28741 tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca tcgagagaat
    28801 cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg cctgcgcgga
    28861 acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc agagtttgcg
    28921 gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg cgatccagtt
    28981 caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg tatttttggg
    29041 tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg atatcactca
    29101 tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc gtgttggtag
    29161 agccgcccat cgcgatatcc agcgtcatag cattttcaaa cgccgctttg ctagcaatat
    29221 tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat tcaacaatgc
    29281 gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc agcgagccgt
    29341 tgcccggctg cgacaggccc agcgcttcgg tcaggcagtt cattgagtta gcggtaaaca
    29401 tcccggagca ggagccgcag gtcggacacg cggaacgttc aacctgatcg ctctgggagt
    29461 cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagt ttgattatct
    29521 gatcggacag tttggttttc ccggcctcca tcgggccgcc ggaaacaaag atcaccggaa
    29581 tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag ttagagatgc
    29641 agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca gcgatcagtt
    29701 cgcgagatgg aagtgaataa agcatccccc cgtggcccat ggcaatccca tcatccaccg
    29761 caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg gcgaccagtt
    29821 taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag ttcacaaccg
    29881 cgataatcgg cttgccaaaa tcggcgtcgg tcattccggt ggcgcgccac agcgcacgag
    29941 cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc atactttatt
    30001 tactcccagt gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt ttgtatttat
    30061 tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa gaggccgaag
    30121 aaggcttgct gaatgcgttt ggtaaccggg ccacaacggc cttcgccaat ctgaataccg
    30181 tctacgctgc gcactggcgt gatttctgcc gccgtaccgg acataaagac ttcatccgcc
    30241 aagtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag ctctttcgcc
    30301 agtttgatga tggcatcacg ggtaataccc ggcagcgcgg aggaggtgaa cggtggggtg
    30361 aacagcacac catctttcac ttcaaacagg ttttcgcctg cgccttcaga gatataaccg
    30421 ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct acccaccagc
    30481 agggaagaga gatagttacc accggctttt gccgcagttg ggatggtgtt tggtgctgcg
    30541 cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc gcccagatac
    30601 gctccccacg ggaaagcggc gataatcaca tcggttgagt atcccgctgg cgggtttact
    30661 cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt gagattgttt
    30721 ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgtga aaccgggaag
    30781 cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg gaatacaacc
    30841 ggtcctttgt gtgagtcgta gcaacggatg ccttcaaaaa ccgaggtgcc atagtgcagc
    30901 gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc attgaaccaa
    30961 atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgttcagg cgcggatttg
    31021 ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca gtttatttaa
    31081 ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga tatttatatt
    31141 ttgtgcatcg ctggcggcag ccatattcat tgagcagacg tggaaaccac gatgacgcac
    31201 cacgcgtaaa acacgttcta aggtttccgg attgaagcga gccgatacat tgacctgatg
    31261 ttgcatcatg ataatttctc caacatttct gaattactgg cacctggcgg caccagcggc
    31321 cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc actgttcagc
    31381 atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc agggatgccg
    31441 aaggcgctgg ctaacatgag gaaatcgggg ttatcggtaa gggtggtttc gctgtatcgt
    31501 tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt atcgagtaag
    31561 acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac attcatcatg
    31621 aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac ttgtgcgcca
    31681 actgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat gaaattttcc
    31741 gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac atctgtggtc
    31801 acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg agcgtagata
    31861 gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc gcagtgttgc
    31921 tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt taaatcacct
    31981 tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat atccatatgg
    32041 ataacactgg cgtgtggtgc gaaggtgttc agtttgccgg tcacccggtc atcaaaacgt
    32101 gcacccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc tttggttccg
    32161 tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc cagccctttc
    32221 agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc cggaactgcc
    32281 tgcgccatcc ccacgccacc gccaacgtac agcatcggtt tttgcgcttt tgccagcatc
    32341 tggcgcgctt gctcgacttc ggcatgtggg aaagtcactt cgttttcaac ggtggtgaac
    32401 cacggttcca gatcgccgct ggctaactgg atatcttttg ggatatcgac cagaaccgga
    32461 ccaggacgac ctgagctggc aacgtcgaat gcttcagcca tgatgcgcgg caactcttcc
    32521 agagactgca ccagaaagct gtgcttggta caggctaacg acaatcccag gacatccact
    32581 tcctgaaatg cgtcggtgcc gataaacggt gcggacacct gaccagtgat ggcaacgaca
    32641 gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc gcccggacca
    32701 gacgtggcga tacatacgcc agttttaccg gtagcacggg cataaccgat agccgctatt
    32761 gccgcaccct gctcatgtcg gcacagcaag tgctccacgc cgccgtcata caatgcatcg
    32821 taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc ctgtgcccgc
    32881 aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg aatcttgaga
    32941 aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta aggtcaaaaa
    33001 aacccccgga cctttcggtg cgggggtctt agttcgttaa ggcttgatct ctaagccttt
    33061 cctcgtccaa gtgcagcccc gcacggtggg ataataatca ccaccacgct aatcacgacc
    33121 aggctaatca ctcgtagaag ggctgtcatt ttgtcttttc ttgcatcttg ttcgaaggaa
    33181 tgcctaaaga gttaccatag attttgcgaa tagcacaaga tatttttggg cctattttta
    33241 tgatggcttt aataatttca atagaaattg ttgtttttta aaaagaaaat agtgagtgaa
    33301 aaaattcaat ttcagtataa atgttgttcc cttcctcgta gttcatcctt tgtccgctgc
    33361 atttttgcga gcatcattcc accgccagaa caactcaact tcacttgcac aaataaacag
    33421 gaatcagcca ctaaaattca cgaattagcg ggctacagca gcgatatctt atgcacataa
    33481 tggtgactca aggagggttt atgtcactgt caattgttca tacccgcgca gccctggaag
    33541 taaatgcgcc cccaatcact gttgaggtac atatcagtaa aggtctaccc ggcttaacga
    33601 tggtgggctt aacagaaaca acggtgaaag aagctcgcga tcgcgtgcgc agcgccatta
    33661 tcaatagcgg atatgaatat ccggcgaaaa aaatcaccat caaccttgct ccagccgatc
    33721 tgccaaaaga agggggacga tatgatttac ctatcgccat tgcgttgctg gcggcctcag
    33781 aacagcttac agccaataag ttagatgaat atgaattagt cggagaacta gcgcttacag
    33841 gcgctctgcg tggcgttccc ggtgcaatct ccagtgcaac tgaagctatt aagtcgggca
    33901 gaaaaattat cgtcgcgaaa gataacgaag atgaagtggg gctaattaac ggtgaaggat
    33961 gcctgatagc cgatcatctg caggctgtct gtgcgtttct ggaaggtaag aacactctcg
    34021 aacgcccgaa accaactgat gcagtatccc gggcgctaca acatgatctc agtgatgttg
    34081 tcggtcagga gcaaggaaaa cgaggactgg aaattaccgc cgctggtggg cacaaccttt
    34141 tactgattgg gccgccggga acaggtaaaa caatgctcgc cagccgtatt aatggcctgt
    34201 tgccagattt aagcaatgaa gaggcactgg agagcgctgc gatattaagt ctggtaaatg
    34261 ctgaatcagt acaaaaacaa tggcggcagc gcccgttccg ctcacctcat cacagtgcgt
    34321 cgttaactgc gatggtgggc ggtggtgcaa ttccagggcc aggtgaaatt tcgttggcgc
    34381 ataacggcgt gctttttctt gatgagctac ctgaatttga acggcgtaca ctggatgcct
    34441 tgcgagagcc gattgaatcc gggcagatcc atctttcacg cacacgagca aaaataacct
    34501 atccagcccg tttccagctt gttgcggcga tgaatcccag ccctaccgga cattatcagg
    34561 gaaaccataa ccgctgcacg ccagaacaga cattacgtta tctcaaccgg ctctcggggc
    34621 cctttctcga ccgcttcgat ctctcactgg agatcccatt accacccccc ggcattttga
    34681 gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa acaacgcgta atggccgcca
    34741 gagagcgcca atttaagcgg cagaataaac tgaatgcctg gctggatagt ccggaaatac
    34801 gccaattctg caaacttgag agcgaagatg cgatgtggtt ggaagaaacg ctgatccatc
    34861 tggggttatc gattcgtgcc tggcagcggt tactgaaagt tgcacgaacc attgctgata
    34921 ttgatcagtc tgacattatc acacgtcagc atttgcagga ggcagttagc tatcgtgcga
    34981 ttgaccgttt gctcatccat ctgcaaaaac tactgacata aaaaaagggc atttcgccct
    35041 ttttattaat cgtcagaatc ggtgtagtct tcagcacctt caacctgcgg tttaccgccg
    35101 gaaagggtgt gaaaacgttt tggacgctta atacgcgtca tatacttgga ccacacgcgt
    35161 tctgcttctg tcactggctc acgttcgcca cggcatactg ctacgaagag tttctcttcc
    35221 tcggtaaccg gctcgcgttt gccaagatcc aactcattga aggcataacc atgacgctca
    35281 agcagttgtg cctctttgat ggtgaaatca ccatgacgag agaatccacg tggataatgt
    35341 ttattgtcga aatatcgatt agtcgtcgta aagctttccg ccatcctgca cgctcctaat
    35401 tctttgaccg agctagttat ggcgcggagt attagttacg cttgacagag tgtaaaacaa
    35461 aacatttaaa tcataacgac aaataatttt gcggagagca ctgtggatac ggaattgtta
    35521 aaaactttcc tggaagttag ccgaacgcgt cactttggtc gagcggctga atcgctctat
    35581 ctgacccagt cagcagtgag ctttcgaatc agacaactgg aaaatcaact gggtgtgaac
    35641 cttttcaccc gccacagaaa caatatccgt ttaaccgctg ccggtgaaaa actactgcct
    35701 tatgcagaaa cgctcatgag cacctggcag gccgcccgca aggaggtggc gcatacctca
    35761 cgacataacg agttttctat cggtgccagc gcctcgttgt gggaatgtat gcttaatcag
    35821 tggttgggac gcttgtatca aaatcaggat gcccatacag gcttacagtt cgaagcgcga
    35881 attgcccaac ggcagtctct ggtaaaacag ctgcatgaac gccagcttga tcttcttatt
    35941 accactgaag cgcccaaaat ggacgaattt agtagtcagt tgctggggta tttcacttta
    36001 gcgctttata ccagtgcccc ttctaaacta aagggagatc ttaactatct gcgactggag
    36061 tgggggccag attttcaaca gcatgaggca ggtttgatcg gtgctgacga agttcccatt
    36121 ctgacaacca gttctgctga actggcacag caacagattg cgatgcttaa tggttgcacc
    36181 tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc ataccgttgt cgatagcaca
    36241 acactttcac ggccgcttta tgccatatgg ctgcaaaata gcgataaaaa tgcgttgatt
    36301 cgcgatcttt tgaaaattaa cgtgctggat gaagtgtatt aatctgaatg gctggcaagg
    36361 atgccggtag aaggatttac ttcggagagg attatttcag ataaaaaaaa tccttagctt
    36421 tcgctaagga tgatttctgg caggggcgga gagactcgaa ctcccaacac ccggttttgg
    36481 agaccggtgc tctaccaatt gaactacgcc cctaattag
//
LOCUS       DAJTYF010000025        35871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTYF010000025 DAJTYF010000000
VERSION     DAJTYF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.025"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..236
                     /locus_tag="OO590_001403"
     CDS             <1..236
                     /locus_tag="OO590_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR6636829.1"
                     /translation="LGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPA
                     LSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            246..884
                     /locus_tag="OO590_001404"
     CDS             246..884
                     /locus_tag="OO590_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6636830.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLGRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            887..2050
                     /locus_tag="OO590_001405"
     CDS             887..2050
                     /locus_tag="OO590_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6636831.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2134..3758
                     /locus_tag="OO590_001406"
                     /pseudo
     CDS             2134..3758
                     /locus_tag="OO590_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3875..4150)
                     /gene="yjfN"
                     /locus_tag="OO590_001407"
     CDS             complement(3875..4150)
                     /gene="yjfN"
                     /locus_tag="OO590_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6636832.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4299..4628)
                     /gene="bsmA"
                     /locus_tag="OO590_001408"
     CDS             complement(4299..4628)
                     /gene="bsmA"
                     /locus_tag="OO590_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6636833.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4810..5559
                     /gene="yjfP"
                     /locus_tag="OO590_001409"
     CDS             4810..5559
                     /gene="yjfP"
                     /locus_tag="OO590_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6636834.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5556..6311)
                     /gene="ulaR"
                     /locus_tag="OO590_001410"
     CDS             complement(5556..6311)
                     /gene="ulaR"
                     /locus_tag="OO590_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6636835.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6419..7483)
                     /gene="ulaG"
                     /locus_tag="OO590_001411"
     CDS             complement(6419..7483)
                     /gene="ulaG"
                     /locus_tag="OO590_001411"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6636836.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7838..9235
                     /gene="ulaA"
                     /locus_tag="OO590_001412"
     CDS             7838..9235
                     /gene="ulaA"
                     /locus_tag="OO590_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6636837.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9251..9556
                     /gene="ulaB"
                     /locus_tag="OO590_001413"
     CDS             9251..9556
                     /gene="ulaB"
                     /locus_tag="OO590_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6636838.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9566..10030
                     /gene="ulaC"
                     /locus_tag="OO590_001414"
                     /pseudo
     CDS             9566..10030
                     /gene="ulaC"
                     /locus_tag="OO590_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
     gene            10044..10694
                     /gene="ulaD"
                     /locus_tag="OO590_001415"
     CDS             10044..10694
                     /gene="ulaD"
                     /locus_tag="OO590_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6636839.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10704..11540
                     /gene="ulaE"
                     /locus_tag="OO590_001416"
     CDS             10704..11540
                     /gene="ulaE"
                     /locus_tag="OO590_001416"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6636840.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11540..12226
                     /gene="ulaF"
                     /locus_tag="OO590_001417"
     CDS             11540..12226
                     /gene="ulaF"
                     /locus_tag="OO590_001417"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6636841.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12355..12630)
                     /locus_tag="OO590_001418"
     CDS             complement(12355..12630)
                     /locus_tag="OO590_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6636842.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12957..13352
                     /gene="rpsF"
                     /locus_tag="OO590_001419"
     CDS             12957..13352
                     /gene="rpsF"
                     /locus_tag="OO590_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6636843.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13359..13673
                     /gene="priB"
                     /locus_tag="OO590_001420"
     CDS             13359..13673
                     /gene="priB"
                     /locus_tag="OO590_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6636844.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13678..13905
                     /gene="rpsR"
                     /locus_tag="OO590_001421"
     CDS             13678..13905
                     /gene="rpsR"
                     /locus_tag="OO590_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6636845.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13947..14396
                     /gene="rplI"
                     /locus_tag="OO590_001422"
     CDS             13947..14396
                     /gene="rplI"
                     /locus_tag="OO590_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6636846.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14467..15030)
                     /locus_tag="OO590_001423"
                     /pseudo
     CDS             complement(14467..15030)
                     /locus_tag="OO590_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            15094..16241
                     /locus_tag="OO590_001424"
     CDS             join(15094..15331,15331..16241)
                     /locus_tag="OO590_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6636847.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
     gene            complement(16260..16463)
                     /locus_tag="OO590_001425"
                     /pseudo
     CDS             complement(16260..16463)
                     /locus_tag="OO590_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(16610..17512)
                     /locus_tag="OO590_001426"
     CDS             complement(16610..17512)
                     /locus_tag="OO590_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6636848.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            complement(17722..18468)
                     /gene="dsbG"
                     /locus_tag="OO590_001427"
     CDS             complement(17722..18468)
                     /gene="dsbG"
                     /locus_tag="OO590_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6636849.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKHGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18840..19403
                     /gene="ahpC"
                     /locus_tag="OO590_001428"
     CDS             18840..19403
                     /gene="ahpC"
                     /locus_tag="OO590_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6636850.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            19648..21213
                     /gene="ahpF"
                     /locus_tag="OO590_001429"
     CDS             19648..21213
                     /gene="ahpF"
                     /locus_tag="OO590_001429"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6636851.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(21334..21762)
                     /gene="uspG"
                     /locus_tag="OO590_001430"
     CDS             complement(21334..21762)
                     /gene="uspG"
                     /locus_tag="OO590_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6636852.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            21983..23220
                     /locus_tag="OO590_001431"
                     /pseudo
     CDS             21983..23220
                     /locus_tag="OO590_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            complement(23451..23861)
                     /gene="rnk"
                     /locus_tag="OO590_001432"
     CDS             complement(23451..23861)
                     /gene="rnk"
                     /locus_tag="OO590_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6636853.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(24090..24896)
                     /gene="rna"
                     /locus_tag="OO590_001433"
     CDS             complement(24090..24896)
                     /gene="rna"
                     /locus_tag="OO590_001433"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6636854.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            complement(25010..26473)
                     /gene="citT"
                     /locus_tag="OO590_001434"
     CDS             complement(25010..26473)
                     /gene="citT"
                     /locus_tag="OO590_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR6636855.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(26524..27402)
                     /gene="citG"
                     /locus_tag="OO590_001435"
     CDS             complement(26524..27402)
                     /gene="citG"
                     /locus_tag="OO590_001435"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6636856.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(27377..27928)
                     /gene="citX"
                     /locus_tag="OO590_001436"
     CDS             complement(27377..27928)
                     /gene="citX"
                     /locus_tag="OO590_001436"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR6636857.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(27932..29464)
                     /gene="citF"
                     /locus_tag="OO590_001437"
     CDS             complement(27932..29464)
                     /gene="citF"
                     /locus_tag="OO590_001437"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6636858.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(29475..30383)
                     /gene="citE"
                     /locus_tag="OO590_001438"
     CDS             complement(29475..30383)
                     /gene="citE"
                     /locus_tag="OO590_001438"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6636859.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACVREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(30380..30676)
                     /gene="citD"
                     /locus_tag="OO590_001439"
     CDS             complement(30380..30676)
                     /gene="citD"
                     /locus_tag="OO590_001439"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6636860.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(30691..31749)
                     /gene="citC"
                     /locus_tag="OO590_001440"
     CDS             complement(30691..31749)
                     /gene="citC"
                     /locus_tag="OO590_001440"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6636861.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            32130..33787
                     /gene="dpiB"
                     /locus_tag="OO590_001441"
                     /pseudo
     CDS             32130..33787
                     /gene="dpiB"
                     /locus_tag="OO590_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            33756..34103
                     /locus_tag="OO590_001442"
                     /pseudo
     CDS             33756..34103
                     /locus_tag="OO590_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(34215..34910)
                     /locus_tag="OO590_001443"
     CDS             complement(34215..34910)
                     /locus_tag="OO590_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCR6636862.1"
                     /translation="MGKEKKSRILTKPCVIEYEGQIVGYGSKELRVETISCWLARTII
                     QTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFKVQPYPK"
     gene            complement(34861..35049)
                     /locus_tag="OO590_001444"
     CDS             complement(34861..35049)
                     /locus_tag="OO590_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6636863.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            35434..>35871
                     /locus_tag="OO590_001445"
     CDS             35434..>35871
                     /locus_tag="OO590_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6636864.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGR"
BASE COUNT         8772 a         8654 c         9420 g         9025 t
ORIGIN      
        1 tgttaggtta cgttatccca ttatccagtg cggcaatcaa tgcggtgagt attccagact
       61 atttcgcctg gggcgggatt gcactggtga ttcagttact cgtttttgct ggtgtcagac
      121 tttatatgcc cgcattaagc gaaaaaatta ttaatcacaa taccgcagca ggaatgttta
      181 tgggaaccgc cgcgctggct ggcggtattt ttaacgcagc ttgtatgaca tggtaatgga
      241 cgatcatggc cagaaaacgc aaatcaagaa ataacagtaa aattggtcac ggagcgatca
      301 gtcgcattgg tagaccgaat aatccttttg aaccgcgtcg caatcgctac gcacaaaaat
      361 acttaacgtt ggcgctaatg ggcggtgccg cttttttcgt attgaaaggt tgtagcgata
      421 gtagcgatgt cgataacgac ggcgacggaa cgttttacgc gacagtgcag gattgtattg
      481 atgacggtaa taatgccgac atttgcgcgc gtggctggaa caacgccaaa gcggcatttt
      541 atgccgatgt tccaaagaat atgactcagc agaactgtca gtctaagtac gaaaattgct
      601 actacgacaa tgttgaacag agttggatcc cggtcgtttc tggatttttg ttaggccggg
      661 ttattcgtaa agatcgcgat gagccgttcg tctataacag cggcggatcc tcatttgctt
      721 cccgccccgt ctggcgcaac acttccggtg attactcctg gcgcttcggt tctggcaaaa
      781 aagagtctta ctcttcaggc ggctttacca ccagaaaagc gtcgaccgtt tcgcgcggcg
      841 gctatggtcg ttcttccagc gcccgtgggc attggggagg ctaatcatgc tgagacacaa
      901 cgttcctgtg cggcgggatc tggaccagat cgccgctaac aacggtttcg actttcatat
      961 catcgacaat gaaatctatt gggacgagag tcgggcttac cgttttactc tgcgccagat
     1021 tgaagagcag atcgaaaaac cgactgcgga actgcatcag atgtgccttg aggtggtgga
     1081 tcgcgcggtt aaagacgaag agatcctgac gcaactggcg atcccgccgt tgtactggga
     1141 tgtgatcgcc gaaagctggc gcgcacgcga tccttcgctg tatggccgga tggattttgc
     1201 ctggtgtggc aatgcgccgg tgaagctgct ggagtacaac gccgatacgc cgacctcgtt
     1261 gtatgagtcg gcttatttcc agtggttgtg gctggaggat gcccggcgca gcggcattat
     1321 tccgcgtgat gccgatcagt acaacgccat tcaggaacgc ctgatttcgc gctttagtga
     1381 gctttacagt cgggaaccgt tttatttttg ctgctgtcag gacaccgatg aagacaggag
     1441 taccgtgctg tacttgcagg actgcgccca gcaggcaggg caggagtcgc ggtttatcta
     1501 cattgaagat ctcggtttgg gcgtcggcgg cgtactgacc gatcttgatg ataatgtcat
     1561 ccagcgtgca tttaagctgt atccgctgga gtggatgatg cgtgacgata acggtccgct
     1621 gctgcgcaag cgtcgcgagc aatgggtgga gccgttatgg aaaagtatct tgagtaataa
     1681 agggctaatg ccgctgcttt ggcgcttctt ccctggtcat cctaaccttc ttgcatcctg
     1741 gtttgagggt gaaaaatcgc agattgccgc tggcgaaagc tatgtgcgta aaccgatcta
     1801 ctcgcgcgaa ggcggaaacg tcaccatttt cgacggtcag aataacgtcg ttgaccacgc
     1861 tgatggtgat tacgccgatg aaccgatgat ctaccaggcg tttcaacctc tgccgcgatt
     1921 tggcgatagc tacacactca tcggtagctg gattgtcgat gatgaagcgt gcggaatggg
     1981 gatccgtgaa gataacacac tgatcaccaa agacacctca cgtttcgttc cgcattacat
     2041 tgctggataa gaatgtttta gcaatctctt tctgtcatga atccatggca gtgaccatac
     2101 taatggtgac tgccattgat ggagggagac acagtgcact ggcaaactca caccattttt
     2161 aatcaaccta taccattaaa taacagcaac ttatacctgt ctgatggcgc gctttgcgaa
     2221 gcggtaacgc gtgaaggtgc tggctgggat agcaattttc tagccagtat tggtcagcag
     2281 ttaggaacgg ctgaatccct tgaactgggg cggctggcga atgtaaaccc gcctgaactg
     2341 ttgcgctacg atgcccaggg gcggcgtctg gacgatgttc gtttccatcc cgcatggcac
     2401 ctgctgatgc aggcgttatg taccaatcgg gtgcacaatc ttgcctggga agaagacgct
     2461 cgctccggcg catttgtggc gcgcgcggcg cgttttatgt tacatggcag gttgaggctg
     2521 ggtcgttatg tccaataacg atgacctttg ccgccacgcc attgttgtta cagatgttac
     2581 ccacgccgtt tcaggactgg accacgccgc tgctgagcga tcgctacgat tctcacttat
     2641 tgccaggtgg gcaaaaacgc ggtttgttga ttggcatggg aatgacggaa aagcagggcg
     2701 gttccgatgt catgagcaac accatccgtg cagagcgttt ggaagatggc tcttatcggc
     2761 tggtggggca taaatggttt ttctcggtgc cgcaaagcga tgcgcatctg gtgctggcgc
     2821 agaccgcggg cggtctgtcc tgcttttttg tgccgcgctt tttgcctgac gggcaacgca
     2881 acgcgattcg cctcgagcgg ctgaaagata agctgggtaa tcgctctaac gccagttgcg
     2941 aagtggagtt tcaggatgcc attggctggt tattggggca ggaaggggaa ggaattcgtc
     3001 tgatcctgaa aatggggggg atgacgcgtt ttgattgcgc cctgggtagc catgccatga
     3061 tgcgccgtgc attttcgctg gcgatttatc atgcacatca acgccatgtt tttggtaatc
     3121 cattgatcca acagcccctt atgcgtcatg tcttaagtcg catggcactt cagcttgaag
     3181 ggcaaacggc gttgctgttt cgtcttgcgc gagcgtggga ccggcgtgcc gatgccaaag
     3241 aagccctgtg ggcgcgttta tttacgcctg cggcaaaatt tgtgatctgc aaacgaggta
     3301 tgccgtttgt ggccgaagcg atggaggtgc tgggcggcat tggttattgc gaggagagcg
     3361 agctgccgcg gctttaccgg gagatgccgg taaacagtat ttgggaaggt tccggcaata
     3421 ttatgtgcct ggatgtgttg cgcgttctca ataagcaagc gggcgtatac gacttattgt
     3481 cggaagcatt tgtggaagtg aaagggcagg atcgctattt tgatcgcgcg gttcgtcgtt
     3541 tacagcagca gctgcgtaag ccagctgaag aactggggcg agagattact catcagctat
     3601 tcctgctggg ctgcggtgcg caaatgttga aatatgcttc tccgccaatg gcgcaggcgt
     3661 ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt gtcagagcag atccagaatg
     3721 atttattgct gcgggcgacg gggggagtgt gtgtgtaagc gtatacgact gatgcgacgc
     3781 tggtttcgat taactaaatg aaatatgtga aaattgtagg ccggacaagg cgctcgcgcc
     3841 gcatccggca ttgttcattt gctgtagatg acactcatgc atacagtatc gcctgtacgc
     3901 gccagttatc aggctgctgg tcttcgtaca tggtaataat gcggtaccat gacgcacctt
     3961 gttcatcggc ctttaacgcc acgatacgtt ccacgtcctg cggatcccct gaaatattgc
     4021 taactgagat ctgacctatt tcattcaggc ccgtcacgca gtcagcactg gcgaattctg
     4081 cagactgtgc cgtggcgctc gcaggataag tcactaattg cagcgagggt gaggcaagta
     4141 attgtttcat cgtcagctcc atttttcgtt tccccacatg ttgcagggca ttccatcgcg
     4201 gtaataaacg cccaagcttc cagccggtaa tttattgtgc acaggtaaac gcatagggac
     4261 gcaaagcaat gtaaagctga ctatgtaagt tgtctgattt atttacggta caaaatggct
     4321 tgtgaatacc actgtcctgt cacgatggtt tcgtccacca tgacgacaac gtaataatca
     4381 gcttttgcag cgacagcttt tgctctgatt tctgctaatg cgtcatccgg agaaccccga
     4441 accatcgtgc ttacgctacc tattcgctgt aacccttgcg tctggtcgcg gcgaatctct
     4501 tgcggatggt ccgttactgg cggtgctggc tgtggcgtac cttgcagtgc gctacaggca
     4561 cttaacatca acaccaataa taaactggca aaccggtaaa taacgctatt acgtttcctg
     4621 ctaaccatag tgtagtgcct tattaattta actttggggg aatgttcccg aattgttacc
     4681 tggtacgcga ttttcgaatg aaaacgtgac gaaagcgtaa cccacatctc aacattatga
     4741 actaataccg ccagcagaaa cttgtgctaa ggatttcttc atgtcatacc agacaaaaag
     4801 gagagacaga tgattgaaat agaatcacgc gagctggcag atattcccgt tcttcatgct
     4861 tatcctgtcg ggcaaaaaga taccccgtta ccgtgcgtaa ttttttatca cggctttact
     4921 tcatccagcc tggtgtatag ctattttgcc gttgcgctgg cgcaggctgg tttgcgggtg
     4981 atcatgccgg atgcgcccga tcacggtagc cgttttagtg gcgacgaagc gcggcggtta
     5041 aatcaattct ggcaaatctt gctacaaagt atgcaggaat tcactacttt acgtgcggca
     5101 atagccgaag aaaactggct gcttgatgac cgtctggcag tcggtggcgc gtcgatgggc
     5161 gcgatgacgg cactgggaat taccgctcgc caccccacgg tgaaatgtac cgccagcatg
     5221 atgggatcgg gctattttac atcgctcgcc cgttcactgt ttccaccgct gatacctgaa
     5281 acggcagcac agcagaatga attcaataaa attgtcgcgc cgctggcaga gtgggaagcg
     5341 acaaaccacc tagaacaact tggggacaga cctctactgc tgtggcatgg cctcgacgat
     5401 gacgttgtgc ctgccgacga atcactacgt ttgcagcagg ccttaagcga gacgggacgg
     5461 gataaactgc taacctgttc atggcagcca ggcgtgcgtc accgcattac gcctgaggcg
     5521 ttagatgctg ccgtgacatt tttccgccag catctttaaa cacgcagaat gctgacccct
     5581 tgcgcttcca gttgttgcag gatttccggg ttagcatttt tgccggtgat cagcatatcg
     5641 atttgatcgg cacggctaaa aagcattccc gcgcgttcgc caatcttact gctatcaacc
     5701 agtaccacca gtttccctac cacgctcagc atcttctgct ctgccattgc tgtcagcata
     5761 tcggttttat acaacccttc tgcggtcagc ccttttccgc tggtaaacat ccagtgcccg
     5821 gcatagagac tgttttcgct gccctgcggg cttaaagtga tggactgact tttgttgtac
     5881 tgtccaccca taatgatcac gctgtcatgt tcttgatcga tcaggtaatt tgccagcggt
     5941 agataattag tgatgatttg cactggcttg ccacacattt cccgcccaag cagaaacgcg
     6001 gtggagccgc agttgatgac tacgctttcg cccggattaa ccagctgcga ggccgcttta
     6061 gcaatacgta ctttttcatc gtgattctgc gcctgatgca gattcatcgg cgtccagcgc
     6121 gggcgctgtt gggtaatagc ttctgcgcca ttgcgcacct ttttcagttt gccgctttcg
     6181 ccaagtttat tgatatcgcg tcgcgcagtg gcaggcgaaa ttcccagacg ctcaacgact
     6241 ttctcaacgg tcacaaagcc caattgtgcg agcatttcca ggaggatttg atgtctttgt
     6301 gcttcagtca tgagctattc cgataaaatt tgattttttt agatgatatt tgaaatagcc
     6361 aggaaatact acgccggata gcgcgaactt ctccacgcta tccggtataa acgacagatt
     6421 acaggaatga cttgaacggc agatccggtt caatagtgaa gcaatcatcg aaaccgcgcg
     6481 gatagtggta ctcgaagttg tctttatcca gcggccaggt aaatttgccg ccaacctgcc
     6541 agataaacgg cttgaagcca tacttcagac gatctttttt catctcccac agcacgcgga
     6601 tctcttgcgg atcggcctgg aagtttgacc agatgtcgtg gtggaacggg ataaccactt
     6661 tcgcattcag ggcttcaccc atacgcagca tatcggcgct ggtcattttg tcggtaatac
     6721 cgcgcgggtt ctcaccatac gatcctaacg ccacatcgat ctgatgttcg ttaccgtgct
     6781 tcgcataata gttagagtag tgggaatcgc cactgtgata tagggaaccg ccaggggttt
     6841 tgaacaggta gtttactgcg cgatcgtcca tgccatctgg cagtacgcca gccgcttttt
     6901 gatcggcagg cagggtgatc agtgcagtac ggtcgaaagc gtcaagcgca tgaatttcaa
     6961 tgtctttcac ttttacaaca tcgcccggtt tgaccacgat gcaacgctct ttcggtacgc
     7021 cccagccaat ccacaaatca acgcaggttt tcggtccaat gaatggtacg tcatcagcac
     7081 agttctgcat cacggcagca gcgacgttaa cgtcgatatg atcgttgtga tcgtgagtcg
     7141 ccagtaccgc gtcgatctgg cgaatcgcaa acggatcaag aacaaacggg gtggtgcgta
     7201 ggtttggctg cagttttttc acgccagcca tgcgctgcat ctggtgaccc tgtttcatta
     7261 acgggttacc gtgactttgt ttgccagtgc cgcaccagaa atcaacgcaa acgttggtgc
     7321 caccttctga tttcaaccag atcccggtgc agcctagcca ccacatcgca aatgtgccag
     7381 gggcgacctg ctcttgttca atttcttcat tcagccagct accccactcc gggaaagtgc
     7441 tcaggatcca ggattctcgg gtgatacttt tcactttact catcgccatt taccttcatg
     7501 atagttcaat tcgaatcaat atgtgattgg ttttgattaa tcctgacact attttttcag
     7561 gaaggcaatg accatttttt gactttttgc cagggaagtt gttgttgatt ttgagtgtag
     7621 aaagatttaa tggaatgtat agttaaatta actaaatgaa tttaaataaa taattttttc
     7681 gttgtgtgaa tcccactcta tccgtgtgga attatttgcg ggtcgcgtca catttaatca
     7741 taaataatct tgttgtgatt acttttgaaa attagagtga gtgcacaaca ttccgggtgt
     7801 gcggaatacc cggttacctc ttcttcagga gatcgttatg gagatcctct acaacatctt
     7861 taccgtgttt tttaaccagg tcatgaccaa tgccccgttg ttgctgggta ttgtgacctg
     7921 tctgggctac atcctactgc gcaaaagtgt cagcgttatt attaaaggca cgattaaaac
     7981 cataattggt ttcatgttgt tgcaggcagg gtccggcatc ctcaccagca ccttcaaacc
     8041 ggtggtggcg aaaatgtccg aagtctacgg cattaacggc gcaatttccg atacttacgc
     8101 gtcgatgatg gcaaccatcg accgcatggg cgatgcctat agctgggtag gttacgcggt
     8161 gttgttagcg ctggcgctga acatctgtta cgtgctgctg cgtcgcatta ccggtattcg
     8221 taccatcatg ctgactggtc atatcatgtt ccagcaggcg gggctgattg ccgttacgtt
     8281 gtttattttc ggctactcca tgtggacaac cattatctgc acggcgattc tggtttcgct
     8341 ctactggggc attacctcca acatgatgta caagccgact caggaagtga cggacggctg
     8401 cggtttctcc atcggtcacc agcaacagtt tgcatcatta attgcctata aagttgcgcc
     8461 gttcctcggc aaaaaagagg aaagcgttga agacctcaaa ctgccaggct ggctgaatat
     8521 tttccacgac aacatcgtct ccacggcgat tgtcatgacc atcttctttg gtgccattct
     8581 gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc aaagtgcact ggacggtcta
     8641 tatcctgcaa accggttttt ccttcgcggt ggcgatcttc atcatcactc agggtgtgcg
     8701 catgtttgtg gcggaactct ctgaagcgtt taacggtatc tcccagcgcc tgatccctgg
     8761 tgcggttctg gcgattgact gtgcggctat ttatagcttc gctccgaacg ccgtggtctg
     8821 gggctttatg tggggcacca tcggtcagct gattgcggtt ggcatcctgg tcgcctgcgg
     8881 ttcctcgatc ctgattatcc caggctttat cccgatgttc ttctccaacg ccaccatcgg
     8941 cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg aagatttgtc tggtgatggg
     9001 gatgatcgaa atctttggtt gcgtctgggt ggtgaaactt accggtatga gtgcctggat
     9061 ggggatggcg gactggtcga ttctggcacc gccgatgatg cagggcttct tctccatcgg
     9121 tatcgccttt atggccgtca tcattgtaat tgcactggct tatatgttct tcgctggccg
     9181 cgcactgcgc gcagaagaag atgcagaaaa acaattggca gaacagtctg cttaataagg
     9241 agttttgatt atgaccgtac gtattctggc tgtgtgtggc aacggacaag gcagttccat
     9301 gatcatgaag atgaaagtgg accagttttt aacccaatca aacattgacc acacggtaaa
     9361 tagctgcgcg gttggcgaat acaaaagcga gttgagcggc gcggatatca tcatcgcttc
     9421 tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa tacgtggttg gcgtgcgcaa
     9481 catgctctct cctgccgact ttggcccgaa actgctggaa gtgatcaaag cgcatttccc
     9541 gcaggatgtg aagtaaggac gcgccatgaa attacatgat tcgctggcgg agaataaatc
     9601 catccgcctg caggctgaag ctgagacatg gcaggacgca gtaaaaatcg gcgttgacct
     9661 gctggtggcg gcggatgttg tcgagccgcg ttactaccag gcgattctgg atgcggttga
     9721 acagcacggt ccgtactttg tgctcgcacc aggcctggca atgccgcacg gtcgcccgga
     9781 agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg aaaaagccgc tggagttcaa
     9841 ccacgaagat aacgacccgg tggatatcct catcaccatg gcggtggtcg atgccaacac
     9901 gcactaggaa gtgggcatca tgcagatcgt caacctgttt gaagatgaag aaaatttcga
     9961 ccgtttacgt gcctgccgta ccgagcagga agtactggat ctcattgacc gcaccaacgc
    10021 ggcagcttaa gaaggaattg aagatgtcat taccgatgtt gcaagtcgcg ctggacaacc
    10081 agactatgga tagcgcctac gaaaccactc gcctgattgc cgaagaagtc gacattatcg
    10141 aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc ggttcgcgat ctgaaagcgc
    10201 tctacccgca caaaatcgtg ctggcagacg ccaaaattgc cgatgcgggc aaaattcttt
    10261 ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt gatttgttgt gcggatatca
    10321 acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa cggcgacgtg cagatcgaac
    10381 tgaccggtta ctggacctgg gaacaggcgc aacagtggcg cgacgcaggt attcagcagg
    10441 tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt ggcgtggggt gaagccgata
    10501 tcactgcgat caaacgtctt tccgatatgg gcttcaaagt caccgtcacc ggtggcctgg
    10561 cgctggaaga tctgccgctg ttcaaaggca ttccaattca tgtgtttatc gccggtcgca
    10621 gcatccgtga tgccgcttct ccggttgaag ccgcgcgtca gttcaaacgt tccatcgccg
    10681 aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat cccgcttggc atctatgaaa
    10741 aagcgctccc cgccggggag tgctggctgg aacgcctgcg actggcaaaa acgttgggct
    10801 tcgattttgt cgagatgtcg gtagatgaaa ctgacgagcg cctgtcgcgc ctcgactgga
    10861 gccgcgagca gcgtctggcg ctggtgaatg cgattgttga aaccggcgtg cgtgtgccgt
    10921 ccatgtgcct ttctgcccat cgtcgtttcc cgttgggcag cgaagatgac gcggtgcggg
    10981 cgcaggggct ggagattatg cgtaaagcca tccagttcgc ccaggacgtt ggcattcgcg
    11041 tgatccagct ggcgggctat gacgtttact atcaggaagc caataacgaa acgcgtcgtc
    11101 gtttccgtga cggcctgaaa gagagcgttg agatggcgag ccgcgcacag gtgacgctgg
    11161 cgatggagat catggattat ccgttgatga actccatcag caaggcgctg ggttacgcgc
    11221 actatctcaa caacccgtgg ttccagctct acccggatat cggcaacctg tcggcgtggg
    11281 acaacgatgt gcagatggag ttgcaggccg gaatcgggca tatcgtcgcg gtgcatgtga
    11341 aagacaccaa accgggcgtc ttcaaaaacg tgccatttgg cgaaggtgtg gtggatttcg
    11401 aacgttgctt cgaaacgctc aaacagagtg gctattgcgg gccgtacctg attgagatgt
    11461 ggagcgaaac ggcggaagac ccggcggcag aagtggtgaa agcgcgcatg gccaaagcag
    11521 gcatggtgga ggcggcataa tgcaaaagct aaaacagcag gtgtttgaag ccaacatgga
    11581 tctgccgcgc tatgggctgg tgacctttac ttggggcaac gtcagcgcta tcgaccgcga
    11641 acgcgggctg gtggtgatca agcccagcgg cgttgcctac gaaaccatga aagcagacga
    11701 tatggtggtg gtcgatatga gcggcaaggt ggtggaaggg gagtatcgcc catcttccga
    11761 cactgcgacg catctcgaac tctaccgtcg ttacccgtcg cttggcggca ttgtccatac
    11821 ccactccact catgccaccg catgggcgca ggcggggctg gcgatcccgg cattaggcac
    11881 cacgcacgct gactacttct ttggtgatat cccgtgtacg cgtggattaa gcgaagaaga
    11941 ggtgcagggt gagtatgaac tgaacactgg caaagtgatt atcgaaacgc tgggcaacgc
    12001 cgagccgctg catacgccgg gaattgtggt gtatcagcac ggaccgttcg cctgggggaa
    12061 agatgcccac gacgcggtgc ataacgcggt agtgatggaa gaagtggcga aaatggcgtg
    12121 gattgcgcgc agtattaatc cacaactcaa tcatatcgac agctacctga tgaataaaca
    12181 cttcatgcgt aagcacggtc ctaacgctta ttacgggcag aagtaaaaca cgcgctgcgg
    12241 aaatttcctt cctcgggaga taactggtct aattccgcag ccgtttttca aaaaaagccc
    12301 cctgcgaagg gggcaaagca aactatggca atgtttcgtt ggttatacct ggtgctagcg
    12361 ataaatatcc gcgctggcgt gcatattgcc gttactcccc ggttcccgca tcagaattac
    12421 gtggtagtac gttgcgcctt gcgcatctgt ttcttcattt aatgcctgac gtgcttcgct
    12481 ttcagtggcg aaattatgat tgatataaat cacgcctaag ctttgcacat cgtccatatt
    12541 tctggcctgg tggttattaa tttcaatggc tgcccatgta tttgcactta gcaaaagcac
    12601 agccagaagg gctaaaactc gactgaacat agatacctcc tcgacggctg actttgtgtg
    12661 ctctcctctg tgatgatctt ctgatttaat tttaatcaat gataaagaag ttgatggtga
    12721 ccatttctga tgcagttgtt caaaaaaaca ctgtgacgga atgtgatgaa cttcaaatca
    12781 gcggtttaga ggttatttgc gaaaggggag atttatttct gctccgccct tgagtttagc
    12841 ggggtatgtc agtactataa cgcgtcattt ttcagccgac ctttaacacg ttccttgcct
    12901 ccccgggatt cggctgaccc agacaggagg ctgaataatc cgtaaggagc aattcgatgc
    12961 gtcattacga aatcgttttt atggtccatc ctgaccagag cgaacaggtt ccgggcatga
    13021 tcgagcgcta cactgctgcc atcactggtg cagaaggcaa gatccaccgt ctggaagact
    13081 ggggccgccg tcagctggct tacccgatca acaaactgca caaagcacac tacgttctga
    13141 tgaacattga agctccgcag gaagtgatcg atgagctgga aactaccttc cgcttcaacg
    13201 atgccgttat ccgcagcatg gttatgcgta ccaagcacgc tgttaccgaa gcatctccga
    13261 tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga tttcgcaaac gaaaccgctg
    13321 atgatgctga agctggggat tctgaagagt aatttctgat gaccaaccgt ctggtgttgt
    13381 ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc atcaggaatt cctcactgcc
    13441 agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg ctttcaccgg caggcatggt
    13501 gtcaaatgcc cgttattgtt agcggacacg aaaaccaggc cattactcac agtataacgg
    13561 tcggcagtcg cataaccgtt caggggttca tttcatgcca caaggcaaag aacggactga
    13621 gcaaaatggt tttgcacgcc gagcagattg aattgataga ttctggagac tagccatatg
    13681 gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg cggaaggcgt tcaagagatc
    13741 gactataaag atatcgctac gctgaaaaac tacatcaccg aaagcggtaa gattgtccca
    13801 agccgtatca ccggtacccg tgcaaaatac cagcgtcagc tggctcgcgc tatcaaacgc
    13861 gctcgctacc tgtccctgct gccgtacact gatcgccatc agtaatcggt cacggtccat
    13921 taatacgact ttgagaggat aaggtaatgc aagttattct gcttgataaa gtagcaaacc
    13981 tgggtagcct gggtgatcag gtaaacgtta aagcgggcta tgctcgtaac ttcctggtac
    14041 cgcagggtaa agctgttcca gctaccaaga aaaacattga attcttcgaa gcacgtcgcg
    14101 ctgaactgga agctaaactg gctgaagttc tggcagctgc taatgctcgc gctgagaaaa
    14161 tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg cgacgaaggt aaactgttcg
    14221 gttccatcgg tactcgcgac atcgctgacg ctgtaactgc agctggcgtt gaagtggcta
    14281 agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac tggcgaacac gaagtgagct
    14341 tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa cgtagtagct gaataattcg
    14401 ttattcaacg agacgtaaaa acgccgacca ttggtcggcg ttttgctttc tatttttcgt
    14461 caggcattaa tttcgcaagt agatccattc accattctct tgtgtccata agtcaggatg
    14521 gtttgctggc gttagatgca tacgatcact ccttatgtct cggtcctgcg gcactcgctc
    14581 gaaacctaaa cccgagtaga ttgcggctga atttgataag gaacgtagac caattttcaa
    14641 atcatggggt aaatcagaat acgcgtttag caatgcagca atgatgaatc cgagggcatt
    14701 agaaacgcct gtaacagtgc gaagttggtc gatataaaga tcgattgttg gtagttcaga
    14761 tgaccagcaa ttaggcattt gggcaggaag ggttacaacg tatcctatcg tttggccggt
    14821 atagtctgga gtactataac caggtcccaa aacaacctcg tgtattgtgt atttactgtt
    14881 gggttcgaag gattcgacat cttgcaataa atatttcagt tctgcggtta acctcgcacc
    14941 cgcgtaagct ttattgtatt tctgggcgtg aatattatct aatgtagctt catggttcca
    15001 tccaaaagaa ttatcaccat tcattgttac tgaatcgcca cgggtttaac agacacctca
    15061 gagtcattta agatggctta aagagaggtg cccatgagcg gtaagcgtta tcccgaagag
    15121 tttaaaactg aagcagtcaa acaggttgtt gatcgcggtt attctgttgc cagcgttgca
    15181 acacgtctcg atatcaccac ccacagcctt tatgcctgga taaagaagta cggtccggat
    15241 tcttccacta ataaagaaca gtcagatgct caggccgaga tccgccgtct ccagaaagag
    15301 ctgaaacggg ttaccgacga acgggacata ttaaaaaaag ccgcggcgta cttcgcaaag
    15361 ctgtccgact gaggtacgcc tttatccgtg acaactcctg ttgctggcct gttcgcctgc
    15421 tctgtcgggt gctggatgtt catcccagtg gtttttacgc ctggcttcag cagccgcatt
    15481 cacaacgcca tcaggcagac ctgagactga caggacagat taaacagttc tggctggaat
    15541 cgggatgcgt ctatggttat cgcaaaatcc atctggatct gcgtgacagc gggcaacagt
    15601 gcggagtaaa cagagtctgg agactgatga aacgtgtcgg aataaaggct caggtcggat
    15661 accgaagccc gcgggcacgt aaaggcgagg ccagtatcgt gtcacccaac aggctccagc
    15721 gacagttcaa tccggatgct cctgatgagc gttgggtaac ggacataacc tacatcagga
    15781 cccacgaagg ctggctgtat cttgccgttg ttgttgatct gttctcacgc aaaattatcg
    15841 gctggtccat gcaatcccgg atgacaaagg acattgtcct gaacgcactg ctgatggctg
    15901 tatggcggcg taatcccgaa aaacaggtgc tggttcattc ggatcagggc agtcagtaca
    15961 caagccatga gtggcagtcg ttcctgaaat cacacggcct ggagggtagc atgagccgtc
    16021 gcggtaactg ccatgataat gcggttgcag aaagtttttt ccagttgttg aaacgtgaac
    16081 ggataaagaa aaagatctac ggaacgcggg aagaagcccg cagtgatatt tttgattaca
    16141 tcgaaatgtt ttataacagt aagcgtcggc atggttctag cgaacagatg tcaccgacag
    16201 aatatgaaaa ccagtattat caacggctcg gaagtgtcta gattatccgt ggcgattcat
    16261 tgcgcctccc agtcgataaa caaaacgcag atttttttgc ccatttgtcg ggcaagttcg
    16321 gctgtcagat gcagcattaa accagagtct ttgccgccag aaaatgagac acaaacgcgg
    16381 ggtagggtgt tgagtgtcta ggtaatacgc tctcgtgcag cttccagaat attgagtgga
    16441 agaggaattt tataaataga catctgaaga cgctctctga acgttacagg gtgaacagtg
    16501 agataagact gttaattata aagtaaacac tgtaaagcag agtgttttat ttttcattcg
    16561 agctgtgaat aaatagatga aagttatagt agctgtctgt agttatatat caaaaactct
    16621 catttagacg gtcaataaat cgggagaaca ccaaattatt taaagaagat ttattatagg
    16681 aaatgtaaag ctttattgaa ggtaacggat gttctagttt tatctctttt aaattaagaa
    16741 agtcacggtg gtagttataa agttcatagg ggactattcc cagtaaatct gtaactgcaa
    16801 tgctattaat tattgtcagc aaagaggaac cacgaaatga aattttgcga ttcataaact
    16861 tctcgtctat ttccatttga atatcatcaa ctcctgctga tttggatatt aattgggtga
    16921 actcttctgc cataatttgt tcataagtgc tattgtcagt aattcgcgga tgcttattac
    16981 tacaaattag agtattgcga attgtatgca atggcatgca aatgacggaa cggctgatca
    17041 ctggcatttg ggtgatcacc agatcggctt tgcggtgaac caacagttct tcagcattct
    17101 ctgctgacat gaggatatca tggcactcaa tctcgacaga ggcatcttgc cgtaggcagc
    17161 ggatcagcat actattatta gagcaagaaa tgagctgagg gccgtagata ataaagtttt
    17221 tctttagttc ggacttattg actatattaa ttgtttgttc aaggcctttg aggtttttct
    17281 caagatggtg atgcagattc aaccctgttg tcgtcggcgc gatacctttt cctgagcgaa
    17341 tgaataaagg gtcattaaat tgcgcacgta agcgttgtaa cgactggctc acggctgatg
    17401 gggtgatata tagtgattca gcagctttgc taatgcttaa atgctggtaa atacactcaa
    17461 atatgacaag aagatttaag tcgaactttt tcaagtcgta gagattggcc atacactact
    17521 ccggtttata tttttcttcc atggtatatt cacttaatca atgttttttg cgtatgtatt
    17581 tttattgatt atgttttttt gcttaactca ggattaagtt tttctaaaat agcacagcca
    17641 cttagggtag agcgagcaat tcatgctttt ttaggatggt tgaaaagcag ggatatagtt
    17701 caaccatcca tgaattacct tttatttatt tcccataatg atattaagcg ttttctgatc
    17761 gggcaacccc acggcctgtt gcagcgtatt ttccttactc atgtaataga tagccggcgt
    17821 gacatttgcc cccagatcgt ccatcagttt ctcattgtca cttaacactt tcatttgctc
    17881 tgtacttacg tttgcaggca cgtttagctt aagcttgcaa ccagaggctt catattgttg
    17941 ccaggttttt gcgggatctt tggaggcaag aattgccgct gctgtcgccg ggctttctgg
    18001 cttgataacc ccaaccaaca atgttcttaa ttgcactttg ccagaatcta cccacgggcg
    18061 cgcctgctgc cagaactgtt tacaatatgg gcagaacgga tcggcgaaga cgtagacaat
    18121 gaccggcgca tcttttttac cgtcgaggag ccagtgggat tgttccatcc gttgccacat
    18181 ttcgcgtccg gctggtgcgt aaatttcttt ttcgataagt gtgttactca ggttttcacc
    18241 tttctcgttg tacatgtaac cagagatagc gtgcttacca tcgggagtca ggtagatggt
    18301 gacgcccata tcctgatact ttccgagata acctttcatt cctccggggg catcgaacgt
    18361 tttgatgatt gtaatgccat gtttttcaat cgcttttact ggggaaggaa gttcctctgc
    18421 gaaggcgatt gcaggaagca gagccagtaa aagtatcttt tttaacatta atttgttctt
    18481 ttcagtcagt gcaaaagtcg agtaaaaggc ataacctatc actgtcatag gtaagagctt
    18541 agatcaggtg attgcccttt gtttatgagg gtgttgtaat ccatgtcgtt gttgcatttg
    18601 taagggcaac acctcagcct gcaggcaggc gctgaagata ccaaagggta gttcagatta
    18661 cacggtcacc tggaaagggg gccattttac tttttatcgc cgctggcggt gcaaagttca
    18721 caaagttgtc ttacgaaggt tgtaaggtaa aacttatcga tttgataatg gaaacgcatt
    18781 agccgaatcg gcaaaaattg gttaccttac atctcatcga aaacacggag gaagtataga
    18841 tgtccttgat taacaccaaa attaaacctt ttaaaaacca ggcattcaaa aacggcgaat
    18901 tcatcgaaat caccgaaaaa gataccgaag gccgctggag cgtcttcttc ttctacccgg
    18961 ctgactttac tttcgtatgc ccgaccgaac tgggtgacgt tgctgaccac tacgaagaac
    19021 tgcagaaact gggcgtagac gtatacgcag tatctaccga tactcacttc acccacaaag
    19081 catggcacag cagctctgaa accatcgcta aaatcaaata tgcgatgatc ggcgacccga
    19141 ctggcgccct gacccgtaac ttcgacaaca tgcgtgaaga tgaaggtctg gctgaccgtg
    19201 cgaccttcgt tgttgacccg cagggtatca tccaggcaat cgaagttacc gctgaaggca
    19261 ttggccgtga cgcgtctgat ctgctgcgta aaatcaaagc agctcagtac gtagcttctc
    19321 acccaggtga agtttgcccg gctaaatgga aagaaggtga agcaactctg gctccgtctc
    19381 tggacctggt tggtaaaatc taaatttcct tcgtctttca cgccatagcg gcgttggcgt
    19441 cgcccgctca ccccggtcac ttacttgtgt aagctcccgg ggattcacag gctagtcgcc
    19501 ttgctctgac gcgaaatact tcggaaattc acctaattct tcgggtgctg cggcacccga
    19561 ttttcttccc cgcaccatga tgcaagctgc atccaggtag ccgcagaggc cgcttgcatg
    19621 atgatgttta aagcccagga gataaacatg ctcgacacaa atatgaaaac tcaactcaag
    19681 gcttaccttg agaaattgac caagcctgtt gagttaattg ccacgctgga tgacagcgct
    19741 aaatcggcag aaatcaagga actgttggct gaaatcgcag aactgtcaga caaagtcacc
    19801 tttaaagaag ataacagctt gccagtgcgt aagccgtctt tcctgatcac caacccaggt
    19861 tccaaccagg gaccacgttt tgcaggctcc ccgctgggcc acgagttcac ctcgctggta
    19921 ctggcgttgc tgtggaccgg tggtcatccg tcgaaagaag cgcagtctct gctggagcag
    19981 attcgccata ttgacggtga ttttgaattc gaaacctatt actcgctctc ttgccataac
    20041 tgcccggacg tggtgcaggc gctgaacctg atgagcgtat tgaacccgcg catcaagcac
    20101 actgcaattg acggcggcac cttccagaac gaaatcaccg atcgcaacgt gatgggcgtt
    20161 ccggcagtgt tcgtaaacgg gaaagagttt ggtcagggcc gcatgacgtt gactgaaatc
    20221 gttgccaaaa ttgatactgg cgcggaaaaa cgtgcggcag aagagctgaa caagcgtgat
    20281 gcttatgacg tattaatcgt tggttccggc ccggcgggtg cagcggcagc aatttactcc
    20341 gcacgtaaag gcatccgtac cggtctgatg ggcgaacgtt ttggtggtca gatcctcgat
    20401 accgttgata tcgaaaacta catttctgta ccgaagactg aagggcagaa gttggcaggc
    20461 gcactgaaag ttcacgttga tgaatacgac gttgatgtga tcgacagcca gagcgccagc
    20521 aaactgatcc cggcagcggt tgaaggtggt ctacaccaga ttgaaacagc ttctggcgcg
    20581 gtactgaaag cacgcagcat tatcgtggcg acgggtgcaa aatggcgcaa catgaacgtt
    20641 ccgggcgaag atcagtatcg cactaaaggc gtgacctact gcccgcactg cgacggcccg
    20701 ctgtttaaag gcaaacgcgt agcggttatc ggcggcggta actccggcgt ggaagcggca
    20761 atcgacctgg cgggtatcgt tgagcacgta acgctgctgg aatttgcgcc agaaatgaaa
    20821 gccgaccagg ttctgcagga caaactgcgc agcctgaaaa acgtcgacat tattctgaat
    20881 gcgcaaacca cggaagtgaa aggcgacggc agcaaagtcg ttggtctgga atatcgagat
    20941 cgtgtcagcg gcaatattca caacatcgaa ctggccggta tttttgtcca gattggtctg
    21001 ctcccgaaca ccaactggct ggaaggcgca gtcgaacgta accgcatggg cgagattatc
    21061 attgatgcga aatgtgaaac caacgtcaaa ggcgtgttcg cagcgggtga ctgtacgacg
    21121 gttccgtaca agcagatcat catcgccact ggcgaaggtg ccaaagcctc tctgagtgct
    21181 tttgactacc tgattcgcac caaaactgca taagaagaag taagattcac ctgcaattgc
    21241 ttagccgccg gggtcaaacc tggcggcttt tttatggcat taaaaagccc tgccgggatg
    21301 ttccaggcag ggcggcataa atacttttat ctgttagcga acgaccagta caggcagatt
    21361 ggcgtggcgg atcacgctcg aggcgttaga acctaacaga tgggtcgaaa tcgatgggtt
    21421 gcgagaaccg ataactacaa catcagcccc cagttcttct gccaactcat tgacttcatc
    21481 ccgcacgcta ccaaaacgga catgttgttt aatgcgggaa ggatcgatgg tgaagtggct
    21541 gaccatcgtt tgcagacgtt cttctgcttc atgttgcaga tgctcttcaa aacgacgcac
    21601 atcagcggca aaacggtgca ggctcaggct ggctgacccg ggtagtacgt gaagtagatg
    21661 aataactccg tcatcctggg cgaggaattc agcgtggcga acagctttgt cgctcaattc
    21721 catttcaaat acatcaactg gcataatgat tgccttatac ataacccttt ctccctgtta
    21781 atcatgaaca aatcattcgc catgattata atatttatcc ctgatatttg tctggttctt
    21841 tttccttacg aacggtttct gtgattaata tattctcact gaacaccagg aattctccca
    21901 aaacctgcgg taccgcccgt tttcccgctg tgatagctac ccttaaagac tgactctttt
    21961 ttgaactgtc tctggaggtt gcatgaaagc attgacttat cacggcccac atcacgttca
    22021 ggtagaaaat gttcccgatc cgggcattga acaggcagat gatattattc tgcgtattac
    22081 ggtaacggcg atctgtggct ctgacctcca tctttatcga ggcaaaatac cccaggttaa
    22141 acatggcgat atttttggtc atgaatttat ggggaagtcg ttgaaaccgg gaaggacgta
    22201 aaaaatttgc aaaaaggcga ccgggtggta attccgttcg tcattgcttg tggcgactgt
    22261 tttttctgtc gattacagca atatgccgcc tgcgaaaata ccaatgcggg taaaggcgct
    22321 gcgctcaata aaaaacagat acctgctccc gcggcattgt ttggttatag tcacctgtat
    22381 ggtggcgttc ctagcgggca ggcggaatat gtccgcgtcc ctaaagggaa tgtggggccg
    22441 tttaaagtac cgcctttgct ttcagatgat aaagcccttt tcctttctga tattctgcca
    22501 acggcatggc aggcagcaaa aaatgtgcag atccaacaag gttcaagcgt tgcagtctat
    22561 ggtgctggtc ctgtgggatt gttgacaatc gcctgtgcac ggttgctcgg tgcggaacag
    22621 atttttgttg ttgatcatca tccctaccgc ttgcgtttcg ccgctgaccg ctacggcgcg
    22681 atcccgatta atttcgatga agatagcgat ccggcacagt caattattga acaaacggca
    22741 ggtcaccggg gcgtggatgc agtaatagac gccgtcggtt ttgaagcgaa aggcagcacc
    22801 acggaaacgg tgctgactaa cctgaaactg gagggcagca gcggtaaagc gttgcgccag
    22861 tgtattgcgg cggtcaggcg tggcggcatt gttagcgtac cgggcgtcta cgctggattt
    22921 attcacggtt tcctgtttgg cgacgccttt gataaagggt tgacgtttaa aatgggacag
    22981 acccacgttc acgcatggct gggagaatta ttaccgttaa ttgagaaagg attactgaaa
    23041 ccagaagaaa ttgttaccca ctatatgccg tttgaagagg ccgcccgggg atatgagatt
    23101 ttcgaaaaac gtgaagagga gtgccgtaag gtgattctgg tacccggtgc acaaagcgca
    23161 gaggcggcgc agaaggcggt ttcaggtctg gtgaatgcga tgccgggggg aacaatatga
    23221 tcgtcaggag tggttttcga ggtaaaggac agccatgacg ataatcgccg ccataatcag
    23281 aaatcctatc aggatgtaaa atgcttctgc catggttatt cccacaaacg aaacggaata
    23341 attttgcagc aaaagtgaac agtgagaacc aggaaaaatt gctgattttg cgtaaagagg
    23401 atgcgagtgc atcctctggg caaagcgagt tatcgcttgt gcagatggga ttaaagcagg
    23461 tagtcgccag cagcttctgg ctggtattcg agttccagca cttcaaggtg ggttgcaacg
    23521 ccgcccggaa gttcccagtg aatggaatcg ccaacgcgca gccccagcag tgcggcacct
    23581 accggagcca taacggaaag ctgagtattg ctatcggtca tttttgccgg atacaccagc
    23641 gtgcgcacac gcacttcgcc atctctaaga ttgcggaatt taacccggct gttcattgtc
    23701 accacgtcgt gtggcatctc ttctggcgaa cacatttggg cgcgatccaa ctctgcgttt
    23761 aacgcgtcgg cgattggcaa accagcatag gcgggttgct ccagcagaat atcgatgcgt
    23821 tcggcatcca ggtcgttaat gatgatagtt ggtctggaca tttttactcc atgtcgtcgg
    23881 tactgcgagt gtcgcagata aacataccca aaagaaaacc ctcaccgtca ggcggcgagg
    23941 gtttaactca catgatgata ctgactgttg ctcactcttt gaagtgattt gcgtcacatt
    24001 cagggaattc ctcaatgcag cgcattatgt ataattctta atcggcatgg ttatggaaga
    24061 cgaattagcg tgttttgtaa atcagaggat taataacccg ctttatcaat cacaaaggtt
    24121 ttgccacagt tacctgggtg aggttgtggc aagaatgagt ttgcagaaag cggagcgttg
    24181 atggcgtcag ctttgatcga gatctgaatt tcagtcaaat acgcagggtt accctggcac
    24241 gttagcttaa ctgctttcac gttctctttt ccccagcttt tggcaaaggc ggcgtcaaag
    24301 tcacggcggc tcactgtttt accgtaatta tccgcaagga attttccggc ttcgctctct
    24361 ttaatttctt gattcaggcg taccatcgta ccgaagtatg cgtccggatc aaaaccaaag
    24421 caggcaccgt gtttggcata ttcgtagcgt tccaggcagg aacgtccgcc agctcctggc
    24481 atgacttcac ttagtttagc ggccgtttcc agtgataacc cggtttccgg cgatgaacac
    24541 attcggctgg cgcgtgcttc tggtagattc gggattgggc gagtagcgca accgaagcgc
    24601 atccagcggc gttcatcaac accacgggca gcaaccgatt taggcaatcc tggccacaga
    24661 ccatgtacgg tcagaaaatc agctttgttg gtcgtttcgg tttgcaggcg acattcatct
    24721 cgttcgttac gatttcgatc gtgttgactc tggcaaaatc cggtttgcca ggagagggcc
    24781 aggacatagc gatcaaaatc gccatactgt tttgcctgca acgctacggc gttggcagaa
    24841 gataagggaa gcagagaaac cgcgagcaac gcggcgttac ggcagaatgc tttcataatg
    24901 ggtgtggaac tcatacatac actgaatact atctattaaa tcataaaaaa gcccgccagg
    24961 tgggcttacc tggcgggcgt gatgatttat tcagcgtttg gcgaacgtat tagttccaca
    25021 tggcgagaat cggccagcca accaacagta acatagagat gtaaatcacc ccgaagattg
    25081 cgccaagacg ccagtaatct tttgatttca catagccaca gccgtaaata atcaccccag
    25141 gaccggttgc atacggcgtc agacagccca tgataccgat agacagcacc agcaggatac
    25201 acagttgttc cattggtacg cccggaatac ctttaccgac ggccagaata accggcagca
    25261 tggttgcggt gtgcgcagac aggctggcaa acaggtagtg tgcaaagtag aacaccagaa
    25321 ccagtacaat caccgttgcg tttggtgaga atccttccag gtgcgtactc atggtaccgg
    25381 cgaaccagtc aataaaacca gaacgagtca ggccgttagc catcacaacc agagttgcca
    25441 ggttgaccag cgtgttccat gcgctgttat agcgggtaat gtctttccac ggcacaacgt
    25501 gcagagccag cattagcgaa actgccagca gaccaaccgc agtagcatta atgacttcac
    25561 tgccaaatac ccacaaacct aagctgagca atacaaggcc aatcagcgtc cactctctgc
    25621 gtgtcagcgc acccatggtt tttagttcat cacccgccca ggttgccact tcttcactgt
    25681 gtgtgatttc cggtttgtac agcacgtagg aaagccacgg cgcaatgata agcaagataa
    25741 ccccaaccgg caggaagcag aggaaccact gcaaccagct aatctggata ccggcaattt
    25801 tgctgacgaa ctccagaccc agcatgtttg gtgccgcacc ggtgacaaac atggatgaac
    25861 tcagactggt actaatgacc atcatccaca tcaaatagcc gccaatacga cgcgcggacg
    25921 gatcgttcgg gaatgattta aacaacggcg gcaggttttt aatgaccgga aaaaccgtac
    25981 ccccggtacg cgcggtgttg gacggtgtaa acggtgccag cagaatgtcg ataatgacaa
    26041 tcgcataacc caacgtcagc gtgcgtttgt ccatgaattt caccaggaaa agggcaatgc
    26101 gacgacctaa cccggaaact tcatacccta atgcaaaaat aaatgcgcca aataccagcc
    26161 ataccgtggt gctggaaaaa ccagccaggc cccatttcag cgcctgtttt tgcgcattaa
    26221 acgctgggtc agctaattct ttggcatcaa agagcaggta attactgcca ataacgcaaa
    26281 tagtaaccgc aataaaactg atcgctgttg ccggaattgg ctcgaggatc atgccgacaa
    26341 tcattgccac aaacacagcg aagtaatgcc atgcctgcgg cggcataccg tcggggacag
    26401 ggataagaaa catgacaccc atcaccacca gtggggccaa tagtttccat atattatctt
    26461 ttgctaaaga catacgggtt ctccgaaaat taatatttcc aaatttatca agtgcttaaa
    26521 taattaaatc tgtgctaaaa accaggtaag gatcagcagg tcggcactgc cgcctggact
    26581 gagatttcgt tcgatacact ccctgtcgaa ctgccggaga taatcgagat cggcgggggt
    26641 tcgaatgccc cctttttgca ataatgtttg cgcctcgcgc tgtagccagc gcaggccccc
    26701 ctcgccaccg cgcgatgcaa cgttggtatc gccgttggta gccatcagta ggagcaaggt
    26761 atcgagcaat gccagttcag gatctaaccc ctgatccagc agagtgaggt aatgcggcaa
    26821 ggcgtggttg atcaccagtg gataacccgc ttcggcttca ccgcgtgcgc cggtaaggcc
    26881 aagctgctgg tacaaccgtt gacctgccgt cagtcgtgaa ttattggtac gcagttcgcg
    26941 atcggtcagg ccacggcaga aacttgccgc cgtagaacaa acggttgttg gcgtaaccgg
    27001 ctggttgagc tgaagcaaac ggccaattgc cgcacatagc agccctaaag aaaaaatgct
    27061 gcctttatgc gtgtttacgc ccgcagtggc gcggaacata tcaccttcgc aagccatacc
    27121 aattgggcgt aatccgtgga gtaccgcttc cggtgccatt tctgcactac aggcaccaaa
    27181 ttcaatgaaa cggggtaacc agccctgaat cgccagcgcg ctgcggtgga aatcttccag
    27241 cgccatatct ttgtgcgcac cgcagttaat gcgatccacg aggcctggtt tcggtgacag
    27301 attgacttca gtcagcatgg cgcgccagcc cagcagggcg tactcatcga ttaatgacgt
    27361 cgcaagcttt gtggttttag ttgacgttgc aggcatcgac atcgttcagc agtgcctcca
    27421 tgcggttgag taaatcggtc agttgatggg tttttccacg cgcgcagacg gctgcgcttt
    27481 gctcgcacaa caggcagcgg cgaggcggca gtgaatagtc gcggcgggag agaatttcgc
    27541 cttcgggcgt caggacatcg atatcccata accgcccgag aggatgacta tgttcaagct
    27601 caatggtggc gagcttgaga tcgcgagccg gggcggcaat gctcaacatg ccctccggcc
    27661 cgctggcgga aaccagtgca gcctgctcct gaatttgcca gccctgcttt gtggctaagg
    27721 cacgcaaggc tgtcacgcca tgattaaaaa ttcggcgtgt gacctcgctg tctttaatcg
    27781 gcccaggcgc aaccacggta aaggagacca gtggaacagg atggtgcttg agccaggcgt
    27841 gttgccgtgc ttgcctttca tcccggctga cgagcagctc gggaattgac accgcatggt
    27901 ggctggcgag ttcaggaagc aggtgcatgg cttattcctt cacctgatgc acaacatcga
    27961 tcaccgagcc atcgcggtaa cgcacaacgg caacgacgcg gtcggtgaat tcaatcggct
    28021 gtggttcacc ggtcagcaga cgcgcacgtt cgcgcagcca ctcaatggaa accactttaa
    28081 tgcccgcttc ctgcagacgt tctgctagtt ccggacgtgc cgggttaact gcgataccgt
    28141 ggtctgtgac cagaatatcg acactggagc ccggggtgat gcaggtcagc acgttatcca
    28201 ccagagtcgg aatacgaccg cgtaccagtg gcgcgacgat gatggaaagc gcagaggcaa
    28261 tcgcggtatc gcagtgacca ccggaagcgc cacgcagtac gccgtcagaa ccggtcagca
    28321 cgttaacgtt gaactgcctg tcaatttcca gcgcgctcag taccaccacg tcgagacgat
    28381 caaccgatgc gcctttcgaa ccccagttag cgtactggtt ggcgctgatt tcgatgtgat
    28441 tggggttacg ggccagcgat tgcgcagcat ggctgtcaaa gctctgcaca tccagcagtt
    28501 tgcggatcag acctttttcg tgcagatcaa ccatcgtcgc ggtaataccg ccgagggcga
    28561 agtcggcgcg aatatcgcgg ctacgcattt tgtcttccag gaaacgggtt accgccagcg
    28621 aagcgccgcc ggtgccggtt tgcatggaga aaccttcttt gaagtagcca gagttgacaa
    28681 tcacatccgc agcgctacgg gcaataagca gttcgcgcgg gttagtggtc atacgggtag
    28741 taccagcgcc gatttttgca gcatcgccaa cgcgatcaac tttgacgatc aaatcaacct
    28801 gatcttgctc aatgcttgcc ggattatgcg gataaggcag cagttcttcg gtaagcatca
    28861 cgacctgttt tgcgttgtcg gcatcaacca ttgcatagcc gagggagccg cagcaggctt
    28921 taccggtgta gccgttggca ttaccgaatt catcacagga cgggacgccg aggaaagcca
    28981 cgtcgatatt cagttcgccg ctctgtacca gatgcacacg accgccgtga gagtggatct
    29041 gcaccggttc cgccagcaga ccacgggaga tctcttccgc cagtggaccg cgtaggccag
    29101 aggtataaat gcgggtaacc acgccctggc gaatgtgttc taccagcggc gcatggcaat
    29161 cactcaggga gctggacgcc agggtcaggt ttttaaagcc catcttcgcg atgacgtcca
    29221 tcaccatatt gacggtcagg tcaccgccac ggaaagcgtg atggaaggaa accgtcatgc
    29281 cgtcctgtaa acctgagcga cgaatcgctt cttccaggtt ggcgcacagt ttgcgatcgc
    29341 gcgctttttc agcctggtag gtttgctttg gtgagttctg gaaagcggca agatcgcatt
    29401 cagtgcgacg attccaggcc gctacccgtt cttgtcgttg agattgttca attttctgcg
    29461 tcattttgat tgccttattc ttcgcggatg ccggaaagtt ctgcacggga gagcaccaga
    29521 cgggcgcgat cgataaccgg accgtccacc atcttaccgt tcagggaaac cacgccgagg
    29581 ccttcgcgag cggcggcttc agcggcttct acgacgcggc gggcgtgatc cacttctttc
    29641 tgggtcggtg cgtagaggtt gtgcagcaga tcaatctgac gcgggttgat cagcgatttg
    29701 ccgtcaaagc ccagctgttt gatgtgggcg gcttcttgca ggaatccggc ttcgttgtta
    29761 gcgtcggaat agacggtatc gaacgcctga atacccgcag agcgcgcggc ctgcaaaatg
    29821 gaacagcgtg cgaacagcag ttcagttcct tccggggagc gttctgtacg caggttgcgc
    29881 acatagtctt ctgcaccgag ggcgataccg atcaaacgct cggaagcgtg agcgatttcc
    29941 actgcgcggg taatgcccag cggagattca atcgccgcca gcaggccggt gctgccgggt
    30001 tcacgaacac aggctttttc tatacgcagg atctcttttt caatatccag aacatcctga
    30061 gcggtatcgg ttttcggcag acgcacaacg tccgcaccac cgcgaacgac ggcttccagg
    30121 tcgttaacac cccattcgga atccagcgcg ttgacacgca caatggtttc aatatcgcga
    30181 tacagcggat gttgcagcgc gtgataaacc atgcggcggg cggtgtcttt ttcacgtaat
    30241 gccacggagt cttcgaggtc aaacatcagg gcatcagccg ggtagatgaa ggagttgctg
    30301 accatcgcgg cattggcacc aggcacaaac aacatgctgc ggcgggtgcg agttttacgt
    30361 tgttgcagcg aagcggaaat cattggcaat cctcccatgg cagagccggg ataccgctgg
    30421 cgcgtgccag cagggcttcc agtcgtgcac gtaaaatgca gtccagtgcg cctttgtcat
    30481 cgacattcag ctgtacgccg cgcacgttgt agcgggcgag aacgtccaga atggtggtgc
    30541 gaattgcatc gccaaactgt ttctcaacgc tgctattgat ttgcaggtcg atatcctgcg
    30601 tatcgagtgg ggcgatgcgt atcatcacat ccccagactc aagggtgcct gcaacggcgg
    30661 gctggtttat tttcattttt cacctgtttc tcatgcgggg gtcttttgac gagctgccgc
    30721 gtcctggcgg gagtgttcaa gcaggttctg caaataatgc agcgtgactg cagggaccag
    30781 cggcgcgata gccgtgagat cgtttttcgc cagcagttga cgtacccggg aagcggatat
    30841 cggcatctcc tggtaacgca gccgctcaat ttcaaccagt tcgatgggcg ctgcggagat
    30901 agtcggcgtt tccagccagt agcgcatatc ctggttgtac tgggcggtaa cgcgacaaaa
    30961 gggttcagta ccgacaaagc ggtgagttac accaagcgcg ggagcgaggt actgacggaa
    31021 aatcttcaga tcaatttcgg tgtaacaatg gttaatgacg ctctgttctt taatgaagta
    31081 gcaagggaac gtagcgcggg agatgatgta ttctgagccg cgatgcacag tcaggcgtgg
    31141 aatatcggcg gtgcctttta acaccagatc cagccggtct tcatagggga agcgtgaaga
    31201 atcttctttg actaaaaaca gatgaaacca gtcgcactgt gccgcagctt gttgaatcag
    31261 ataacggtga ccattcgtaa agggattggc gttcatcaca atgcagccaa tcttgttccc
    31321 tggatgacgc aattttttca gcgattcggc atagcgtttc agtcgcgtgg cgctgttttc
    31381 catcagcacc atcacgccgg gtacgcaggt cagcgtggaa aaaccgcact ggcggaacag
    31441 cgcctcgtat tcggttttgg tataaataaa cagatgcgtg ctgtgccgct cataggcgag
    31501 gtttatcagt tcagtggcta atgtcagcgc cagtccttca ccgcgaacgg attcactgat
    31561 agcaacgcat ttaataatat ttccggcaat tccaccgcac gcgataagct tttcatcgcg
    31621 ggttacggta ataaatactt cgactgtggt gtcaacgctc aaatcatttt catgcaggaa
    31681 ttgggcgatt tccgccattt ttttattttc tgaacgtttt acgcgggtga aaatatcatt
    31741 gacgaacata ataaatagta tcctgaaggt gcatgttgtt atcgatttgc aacgaatgtt
    31801 gttcaatgtt gcaaactgat aaccttttat tttcacttgg gagaaagggg ggtgatcgag
    31861 gtatatcttt ttctcctttc gctatacatc ctaaggagta tttcggcgtg aaattttgat
    31921 ttatttcaca tagagttagt ggttttttta tttgtttaat ggttttaagt tttttaatta
    31981 atgtaattac gaaatgactc gcaggtttaa gtgatttaat tgatttaatg aataaaattt
    32041 gccacgatca taattaatat ctatgtattt tgattcaaca ttttaattac atccgtcaaa
    32101 gaggctcggg acaacccgca aggaaaacaa tgttgcagct taacgagaat aaacagtttg
    32161 catttttcca aagactgaca tttccgctgc gtatcttttt gctgattctg gtgttctcaa
    32221 tatttgtcat tgcagccctg gcgcaatatt ttacggccag ttttgaggac tatttaacgc
    32281 ttcatgtacg cgacatggca atgaatcagg cgaaaattat tgcctccaat gacagtgtca
    32341 tctctgcggt gaaaacgcgt gactacaaac ggctggcgac catcgctaac aaattacaaa
    32401 gagataccga ttttgattat gtggtgattg gcgaccggca ctcgatccgc ctttaccatc
    32461 ctaatccgga gaaaattggt tatcctatgc agttcaccaa acagggcgcg ctggagaaag
    32521 gggagagcta cttcattacc gggaaagggt caatgggtat ggcgatgcgc gccaaaacgc
    32581 caatctttga tgacgatgga aaagtcatcg gcgtggtgtc gattggctac ctggtgagta
    32641 aaatcgatag ctggcgggct gagtttttat taccgatggc aggtgtgttt gtcgtgctgt
    32701 tagggattct gatgttgctg tcgtggttcc tggccgcgca tatccgtcgg cagatgatgg
    32761 gcatggagcc aaagcaaatc gcacgggtgg tccgtcagca agaggcgctg tttagttcgg
    32821 tttatgaagg gctgattgcg gtggatccgc acggttacat taccgccatc aatcgtaacg
    32881 caagaaagat gctggggctg agttcccctg gacggcaatg gttgggtaaa cccattgctg
    32941 aagtggtcag gtctgccgat ttctttaccg aacagattga tgaaaaacgt caggatgtgg
    33001 tggctaactt taacggtctg agcgttattg ccaatcggga agctattcgt tcaggtgatg
    33061 atttgctggg agccattatc agctttcgca gtaaagacga aatttccacc ctcaatgcgc
    33121 aactgacgca aataaaacaa tacgttgaga gccttcgtac attgcgacac gagcatctca
    33181 attggatgtc gactctcaat ggtctgttgc agatgaaaga gtatgatcgc gtgctggcga
    33241 tggtgcaggg ggagtctcag gcccagcaac agcttattga cagcctgcgc gaggcgtttg
    33301 ccgatcgcca ggtggcgggg ctgctttttg gtaaagtgca gcgcgcccgc gaactggggc
    33361 taaaaatgat catcgtcccc ggtagccagc tttcgcaact gccgccagga ctggatagca
    33421 ccgagtttgc cgccattgtt ggcaatttac ttgataacgc cttcgaagcc agcctgcgta
    33481 gcgatgaagg aaacaagagc gttgaattat tcctcagcga tgaaggcgat gatgtggtga
    33541 ttgaagtcgc cgatcagggc tgcggcgttc cagagtctct acgagacaaa atatttgagc
    33601 aggggtcagt acgcgtgctg acgagcccgg tgaacatggc attgggttgt acttgattgc
    33661 cagctacgta acgcgctgcg gtggtgttat cactctcgaa gataatgatc cctgcggtac
    33721 cttattttca atctatattc cgaaagtgaa acctaatgac agctccatta accctattga
    33781 tcgttgagga cgaaacgccg ctggcagaga tgcatgcgga atatattcgt cacattcccg
    33841 gattcagtca gatattactg gcgggaaacc tggcgcaggc ccgaatgatg atcgagcgtt
    33901 ttaagccggg gctaatcttg ctcgataact atcttcctga cggtagaggg attaatttac
    33961 tgcatgaact ggtgcaggcg cattatcccg gcgacgtggt gtttaccact gcagccagtg
    34021 atatggaaac ggtgtctgaa gccgtacgtt gtggtgtatt tgattatctc attaagccaa
    34081 ttgcctatga acggctgggt gaagcggcgc acgaaaaacg cgaaagcgtt tcacgataaa
    34141 tgcgaaaact ttagctttcg cgcttcaaat gaaacagcag tattaattac cgctttttat
    34201 tcattacatg gggatcactt agggtatggc tgaaccttga atagtttggt atttagccgc
    34261 ttccttgctc gcttgttcag gaatctgatg tagcgatact gattaaattt gtgcaccacg
    34321 gcacgctcct tattagctcg caaatactcg cctcgttgcc ctccgcgctt aatcgcgttc
    34381 atcgttatct cgtgatacca ctcaccatcc agctcgtaga acgtgctttc atggctgcca
    34441 ataaaatcaa aattcgacgc ctgatacaca acgcctgcga gtccgcagcg ttcgtcagca
    34501 aaggactgaa cccactccac tgacggatac agtaatctga taactttcag cgcgtagctg
    34561 atggcccgtg attcagagtt gcggggcatg tcgtcgtgta gccacatgcg attcaactcc
    34621 atatagcccc ggttatccgt ttcaagcacg acacgacgac ctgagttggg gttaagggca
    34681 tatccccact ggagaacgcc aaccagatca cgcccgctga atactcccag gtgaaggtaa
    34741 gagttattca caaaacggcg ggaatagtgc tttgtctgaa tgattgtgcg ggccagccag
    34801 caggatatgg tttcaacgcg cagctccttt gaaccatagc caacaatctg gccttcatac
    34861 tcaataacgc acggtttcgt cagaatgcgt gatttcttct cttttcccac aatgtttctc
    34921 cgtgggatgc tcgcaggcat tcagcattat gatgtgacgt ttgcaacgcg gacacctgat
    34981 ttcaatgtga tcaaaggaat ccgctttaaa taacagtttg ttgcagttct tacagcgaat
    35041 tgatttcatt tcacctcctt gcatcaattc gccgttatct taaaaaatct catcagtcgg
    35101 gtgtggttat cgggacataa tcgatcggta atacagatcg atttaattga ttcgatcgtc
    35161 gttttctata tgcgttcctt tttgaaggcg ctttaaagca cgttttaatc ccggtcggtc
    35221 tgtccttgtt ccgcttaatt tatcttcaaa tatttattca catcctgcac aaacaagagc
    35281 gtttcgttgc aggtctgtat tctggtcatt tgttgatacc cttacatagc caatcagcac
    35341 gctgaattcc ccgtttaaaa gcggaaatca tgccatgcag gccagaaacg gcccctcggt
    35401 ttacaggaaa cggtaaacaa ggctggtaac gccgtgcaaa aaaatggcga tacgttgtcc
    35461 ggtggactta cttttgaaaa cgactcaatc cttgcctgga ttcggaatac tgactgggca
    35521 aagattggat ttaaaaatga tgccgacagc gatactgatt catacatgtg gtttgaaaca
    35581 ggcgacaacg gcaatgagta tttcaaatgg agacaccacc tcactggcgg ccgggttaaa
    35641 gacctgatga atcttaaatg ggatgctctg tatgttcttg ttaaagccct tttcagcagt
    35701 gaagtaaaaa tatcgacggt caatgcactg agaatattta attcatcttt tggtgccatt
    35761 tttcgtcgtt ctgaagaatg cctgcatatc atccctacac gagagaatga aggggaaaat
    35821 ggtaatatag ggccactacg cccctttacg cttaatctca gaactggccg g
//
LOCUS       DAJTYF010000026        35825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTYF010000026 DAJTYF010000000
VERSION     DAJTYF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.026"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..218)
                     /locus_tag="OO590_001446"
     CDS             complement(<1..218)
                     /locus_tag="OO590_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6636865.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAEVY"
     gene            complement(219..305)
                     /locus_tag="OO590_001447"
                     /pseudo
     CDS             complement(219..305)
                     /locus_tag="OO590_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(399..1874)
                     /locus_tag="OO590_001448"
     CDS             complement(399..1874)
                     /locus_tag="OO590_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6636866.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2021..3305)
                     /locus_tag="OO590_001449"
                     /pseudo
     CDS             complement(2021..3305)
                     /locus_tag="OO590_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3418..5352)
                     /gene="yeaG"
                     /locus_tag="OO590_001450"
     CDS             complement(3418..5352)
                     /gene="yeaG"
                     /locus_tag="OO590_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6636867.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGTLCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILPRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5788..6534
                     /gene="mipA"
                     /locus_tag="OO590_001451"
     CDS             5788..6534
                     /gene="mipA"
                     /locus_tag="OO590_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6636868.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6624..7385
                     /locus_tag="OO590_001452"
     CDS             6624..7385
                     /locus_tag="OO590_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6636869.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7436..8320)
                     /locus_tag="OO590_001453"
     CDS             complement(7436..8320)
                     /locus_tag="OO590_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6636870.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8404..9399)
                     /gene="gapA"
                     /locus_tag="OO590_001454"
     CDS             complement(8404..9399)
                     /gene="gapA"
                     /locus_tag="OO590_001454"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6636871.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9741..10154
                     /gene="msrB"
                     /locus_tag="OO590_001455"
     CDS             9741..10154
                     /gene="msrB"
                     /locus_tag="OO590_001455"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6636872.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10196..10468
                     /locus_tag="OO590_001456"
     CDS             10196..10468
                     /locus_tag="OO590_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6636873.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10838..11914
                     /locus_tag="OO590_001457"
     CDS             10838..11914
                     /locus_tag="OO590_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6636874.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11941..13320
                     /locus_tag="OO590_001458"
     CDS             11941..13320
                     /locus_tag="OO590_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6636875.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     SLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            13337..14382
                     /locus_tag="OO590_001459"
                     /pseudo
     CDS             13337..14382
                     /locus_tag="OO590_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14403..15239
                     /locus_tag="OO590_001460"
     CDS             14403..15239
                     /locus_tag="OO590_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR6636876.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15244..16192
                     /locus_tag="OO590_001461"
                     /pseudo
     CDS             15244..16192
                     /locus_tag="OO590_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            16202..17182
                     /gene="ydjG"
                     /locus_tag="OO590_001462"
     CDS             16202..17182
                     /gene="ydjG"
                     /locus_tag="OO590_001462"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR6636877.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17319..18077
                     /locus_tag="OO590_001463"
     CDS             17319..18077
                     /locus_tag="OO590_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6636878.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18195..19553
                     /locus_tag="OO590_001464"
     CDS             18195..19553
                     /locus_tag="OO590_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6636879.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19646..20287)
                     /gene="pncA"
                     /locus_tag="OO590_001465"
     CDS             complement(19646..20287)
                     /gene="pncA"
                     /locus_tag="OO590_001465"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6636880.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20298..21314)
                     /gene="ansA"
                     /locus_tag="OO590_001466"
     CDS             complement(20298..21314)
                     /gene="ansA"
                     /locus_tag="OO590_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6636881.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TLDD"
     gene            complement(21481..23337)
                     /gene="sppA"
                     /locus_tag="OO590_001467"
     CDS             complement(21481..23337)
                     /gene="sppA"
                     /locus_tag="OO590_001467"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6636882.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23498..24049
                     /locus_tag="OO590_001468"
     CDS             23498..24049
                     /locus_tag="OO590_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6636883.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24166..25209
                     /gene="selD"
                     /locus_tag="OO590_001469"
     CDS             24166..25209
                     /gene="selD"
                     /locus_tag="OO590_001469"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6636884.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25214..27175
                     /gene="topB"
                     /locus_tag="OO590_001470"
     CDS             25214..27175
                     /gene="topB"
                     /locus_tag="OO590_001470"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6636885.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            27302..28342
                     /locus_tag="OO590_001471"
     CDS             27302..28342
                     /locus_tag="OO590_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6636886.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQLGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28459..29802)
                     /gene="gdhA"
                     /locus_tag="OO590_001472"
     CDS             complement(28459..29802)
                     /gene="gdhA"
                     /locus_tag="OO590_001472"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6636887.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            30038..30310
                     /locus_tag="OO590_001473"
     CDS             30038..30310
                     /locus_tag="OO590_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6636888.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(30276..30683)
                     /gene="nudG"
                     /locus_tag="OO590_001474"
     CDS             complement(30276..30683)
                     /gene="nudG"
                     /locus_tag="OO590_001474"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6636889.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30770..31435
                     /locus_tag="OO590_001475"
     CDS             30770..31435
                     /locus_tag="OO590_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6636890.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSL
                     QRQ"
     gene            complement(31444..32751)
                     /gene="ynjE"
                     /locus_tag="OO590_001476"
     CDS             complement(31444..32751)
                     /gene="ynjE"
                     /locus_tag="OO590_001476"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR6636891.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGGWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(32818..33471)
                     /locus_tag="OO590_001477"
     CDS             complement(32818..33471)
                     /locus_tag="OO590_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6636892.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33471..35005)
                     /locus_tag="OO590_001478"
                     /pseudo
     CDS             complement(33471..35005)
                     /locus_tag="OO590_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34978..>35825)
                     /locus_tag="OO590_001479"
     CDS             complement(34978..>35825)
                     /locus_tag="OO590_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6636893.1"
                     /translation="KEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGA
                     QLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPA
                     ALKEAPDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTF
                     NPAHAQQKIASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDA
                     QLRKADPVVWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLR
                     RYGTH"
BASE COUNT         9081 a         8481 c         9171 g         9092 t
ORIGIN      
        1 cagtacacct cagcgagagc gcgcatcaca tgcgcggcaa tactctgatt ctgatactta
       61 tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc actcattgct
      121 ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat gacacttgcg
      181 gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac tccttcggtt
      241 agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc gctaattata
      301 ctcatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa ttgtatgcaa
      361 aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatattatg aataatcact
      421 ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc
      481 gagatattac gcgtgtcacc cgttgaaaaa aacgtgccaa tactgatggt catttgttca
      541 ggaacatcat atctgttact gaagccggtc aggagttcga catttttacg catacgttcc
      601 gcaatgattt ttgcctgggc actattgagt tcggtaaata gcaagccaaa cacctcaccc
      661 tccagtcgcg ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa
      721 accagcactt tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta
      781 atatcaacca gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg
      841 ctgttgaaaa agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg
      901 ctggagagtt gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta
      961 ctggagactt caatcccgcg actgatgtac cagatggtgt aattatactc atccagagtc
     1021 agtagaagta agttgcagca tagcgatgcc agacataata atgctattaa tggccatagt
     1081 tcgctggcta atcgtgtacg catgataata aagaacagta aaaatgccca cagacaaacc
     1141 aatatttttg tatagttgat tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg
     1201 acatacaaag aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact
     1261 aaaaaaggaa tcagcgccag caacagaatg ccagtttttt tcttattgtt gtcaaggatg
     1321 ttgtctttac cataacaaaa gagcgccaac gaggttaaac aaataaaact gagttggcgg
     1381 aaaatataga aaatagaaac atcattcgac tttgtctgga tcagtgtact gtcgctgata
     1441 ggtttatgga taacaacaat agtttcaact aaatagataa ggccacttaa aagtgcaaga
     1501 cttaaaatga gtgttggtag aatttgtttg ttagtgaaat atttcattaa catgaaaaga
     1561 aaaatgattg cgttaaggaa aaacaaagcc acaacgacca tcagataatt catttgtggg
     1621 atgagtttta cattcgtctt ttcgttatat aatgtggtga tgcaaaataa aattaacgtc
     1681 agcaataaac tgaaaataaa aaagctttga gcttttaatt tttgtgtgcc catagaaacc
     1741 ataaaaagga gagcaatgtg agtgctggat tttattgatg gcagagatat atagttttga
     1801 ccaagatcaa ttttcgttaa cgataaaaaa tatttaaaga ataaccccag ggaaatacgt
     1861 gttgactgaa tcatttttcc gaatgattgt tttctggaac taaattgtgt aaaggttatg
     1921 ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa tttacagtaa
     1981 gctaaaaatc agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat
     2041 tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct
     2101 gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgcaca caatgtctga
     2161 tgtgcacgac gggtaatttc gatatagctg taataacgaa caacaggtaa taattttttc
     2221 gccaggattt catggcaaag cggagagtca tcggcccagt tatcgccgtc cgatgcttgt
     2281 gcagcgtaaa tattccactg tgccgggtta taacgctctt tcactacctc atccatcagt
     2341 ttcagggcgc tggaaacaat ggtgccgcct gtttcctgcg agtagaaaaa ctcatgttca
     2401 tcgacttctt tcgcctgggt atgatggcgg atgtatacga cttccacgtt cttatacgtt
     2461 ctgctgagga acagatacag cagaatataa aaacgcttag ccatatcttt agtggattga
     2521 tccattgaac cggaaacgtc catcaggcaa aacatcactg cctggctgga gggatcgggc
     2581 cgcttctcgt agttcttgta acgtaaatcg aaggtgtcaa taaaagggac gcgttcaatt
     2641 ttggcacgta attctgcaat ttctttgcgc agacgttcct cttccagcag ctgcgcaggt
     2701 tcactgttgc tgatgatggc caaattctct tccagtgcat gaagttcccg ccgcttgcct
     2761 gccgtcatgg ctgtgcgtcg cgccagtgag ttctgcaagg aacgcacaac gctgatattg
     2821 gctggaacgc cgttagcggt ataacccgcc cgatgcgttt tatattcggt cagctggcgt
     2881 tgttggtttt gtttcagatt cggtaaggcc aaatcttcaa agagcagatc aagatactca
     2941 tctttcgaaa tctgaaagac aaattcatcc tgaccttcac catcctggct ggcctggccc
     3001 tgaccactgc cggaacctcc gccgccaccc tggggacgtt caattcggtc gttctgaacg
     3061 aaatggtcat tgcccggatg cacgcggtgg cgcagaccgc cacgcccctg atgaaacatt
     3121 ggttcgctaa tatcttccgt gggaatggat acggattcgc tgctgtcgac gtcagtcacc
     3181 gaacgcttat taatggcctc ggagatcgac tgtttaattt gcgctttata acggcgtaaa
     3241 aaacgctggc gattcaccat gcttttgttt ttgccgttca gacgccggtc aataaaccag
     3301 gtcatatgcc ccccgtacta catttgccaa ctgtatgcta cgcctgtgtt gccgcttctc
     3361 aggcccacgg ttcgctatcg ttgtaggccc ggtagcatgt gtaccgggcc agggttgtta
     3421 agacgattta cgtacgcgca aataccattc gcacagtaaa cgcacctgtt tacgggtgta
     3481 gcctttttcc atcatacggt cgacaaagtc gtcgtgtttc ttctgctcgt cggttgacgt
     3541 tttggcgtta aacgagataa ccggcaacag ctcctcggta ttggagaaca ttttcttctc
     3601 gataaccgtg cgcagttttt catagctggt ccagttcgga ttgcgtccgc tgttattcgc
     3661 tctggcgcgc agtacgaagt taacaatctc gttgcggaaa tcttttggat tactgatccc
     3721 cgccggtttc tcgattttct ccagctcggc gttaagagac tcgcggtcaa acagctgccc
     3781 ggtatccgga tcgcgatact cctgatcctg aatccagaaa tccgcgtagg taacataacg
     3841 gtcgaaaatg ttttgcccat attcggaata ggattcaagg taggccgtct ggatctcttt
     3901 gccgataaac tcggcatatt tcgggatcag ataacctttc aggaactcca gatagcgttc
     3961 tgcctgctct tgcgggaact gctcgcgctc gatctgctgt tccaggacgt agaacagatg
     4021 gaccgggttt gccgccactt ctacatgatc gaagttgaac acgcggggga ggatcttaaa
     4081 cgcaaaacgc gtcgacagac cgttcatccc ttcatcgaca ccggcgtagt cacgatattc
     4141 ctgatacgac ttggctttcg gatcggtgtc tttcagactt tcgccatcat aaacccgcat
     4201 ctttgaataa atgctggagt tttctggctc tttcaggcgc gaaagaatgg aaaaacgtga
     4261 cagtgtttcg agcgtgccag gggcgcatgg ggcgtgagtc aattcactgt gattaagcaa
     4321 tttctcgtag attttgatct cttcggaaat gcgcaagcaa tacggcacct tcacgatgta
     4381 aacacggtcg aggaaggctt cgttgttttt gttattacgg aaagtgaccc attcggactc
     4441 gttcgagtgg gcgagaataa tcccgttgaa cggcagggcg gagatacctt ccgtcccgtt
     4501 gtagttatct tcctgagtgg cggttaacaa gggatgcagc acttttatcg gtgctttaaa
     4561 catctcaacg aattccatga tcccctgatt ggcgcggcac agcgtaccgg aatagccgta
     4621 ggcgtccggg tcattctgcg cgtagtgttc gagtttacga atatcgactt tcccaaccag
     4681 cgcggagatg tcctggttgt tctcatcacc gggttccgtt ttggcgatag caatttgttg
     4741 cagaattgac ggccagacct tcactacccg gaacttagtg atatcgccac caaattcatg
     4801 caggcgtttt gccgcccatg gcgacatgat ggtgccgaga taacggcgag gaatgccata
     4861 ctctttttcc agaatctgag catcttcctg cggattgaaa agacagaacg gatgatcgtt
     4921 gaccgggcta cgctcaccgt tggcgctcaa tacataaatc ggtacgagct gcattaatga
     4981 tttcagtcgc tcagcaagcg atgatttacc cccacccaca ggccccagca gatacaggat
     5041 ttgtttcttc tcttccagcc cctgagccgc gtgtttcaga taagagacaa tctgttcaat
     5101 cgcgtcttcc atgccgtaaa actcttcaaa tgccggataa cgtgcaatga cccggttaga
     5161 aaagagtcga gaaagtctgg gttcctgggc tgtatcgacc atgacaggct caccgatagc
     5221 catcaatagc cgctcagccg cgttggcata agcactgcga tcttgccgac aagtggtaag
     5281 aaactcctgc agtgtgaact cttcgtcctt ggcagcttca tatcgctggc gatagtgatc
     5341 gaatatattc atggtatgcc gtcctttcgt tttttagcac aggttaagag ccgttcatat
     5401 gaatagtaga ggctcccgga agaggtaaac tgaacgacgt ctcttgctaa aacacagcaa
     5461 ctctcatgcc attttatcga cgatgagaac aacgtgcccg gtgatacacc ttcaaaatta
     5521 agcgtagatg gcaaatggaa aacttgcctg cacggctttc ttaatttcaa tggcatatca
     5581 acaactcatc cataaatttt gcataattaa tgtaaagacc aggctcgcca gtaacgctaa
     5641 atccatttgg ctgtaagcgc ggtgtcatcc gcggcaggaa aattaaacag ttactttaaa
     5701 aaatgaaaac gtaaaaaggt tgggtttcga tgtattgacg ggtaaacttt gtcgcccgct
     5761 aaacatttgt ttaaggaatg attaattgtg accaaactca aacttctggc acttggagtg
     5821 cttatcgcaa cgtctgcagg cgtagcgcac gctgaaggta aattttccct gggcgcaggc
     5881 gtaggtgtcg ttgagcaccc atataaagat tacgataccg atgtttaccc agtaccggta
     5941 atcaactatg aaggcgataa cttctggttc cgtggcttag gtggtggtta ctacctgtgg
     6001 aatgacgcaa cggataaact ttcaattacc gcttactggt cgccgcttta cttcaaagcg
     6061 aaagacagtg gcgatcacca aatgcgtcac ctggatgacc gtaagagcac catgatggct
     6121 ggtctgtctt atgctcactt tacccagtac ggttacctgc gtaccaccct ggctggcgat
     6181 accctggata acagcaacgg cattgtctgg gatatggctt ggttgtatcg ttacaccaac
     6241 ggtggcctga ccgtgactcc gggtattggt gtgcagtgga acagcgaaaa ccagaacgaa
     6301 tactattatg gcgtatcgcg caaagagtcc gctcgcagcg gtctgcgtgg gtataaccca
     6361 aatgacagct ggagccctta ccttgagctg agcgccagct acaacttcct cggcgactgg
     6421 agtgtttacg gtaccgcgcg ctacacccgt ctgtctgatg aagttactga cagcccgatg
     6481 gtggataaat cctggactgg cctgatttct accgggatca cctacaaatt ctgataatgc
     6541 atctttccag ggcattttgt gcataaaaat agggcgtcac gccctgtttt gcattacggt
     6601 ggtgcaatac ctgggaggaa acaatgcaac aaaaaatgat tcaatttagt ggcgatgtct
     6661 cactgccagc catagggcag ggaacatggt atatgggcga agatgccagt cagcgcaaaa
     6721 cagaagttgc tgcactacgc gcaggcattg aactcggttt aaccctcatt gataccgccg
     6781 aaatgtatgc cgatggcggt gccgaaaagg tggttgggga agcattaacc ggtctgcgag
     6841 agaaggtctt tctcgtctct aaagtctatc cgtggaatgc tggcgggcaa aaagcgataa
     6901 atgcatgcga agccagttta cgccgtctca atactgatta tctcgatctc tacttattac
     6961 actggtctgg cagtttcgct tttgaagaga ctgttgctgc gatggaaaaa ttgatcgccc
     7021 agggaaaaat ccgtcgctgg ggcgtttcta accttggtta tgccgatatg caggaactct
     7081 ggcagctgcc ggggggaaat cagtgtgcca ctaatcaggt gctttaccat ctcggttcac
     7141 gagggattga gtacgatcta ctcccctggt gccagcaaca gcagatgccg gtgatggctt
     7201 acagtccatt agtccaggcc gggcggttgc gcaatggact gttaaaaaac gcggtagtca
     7261 acgaaattgc acatgtccaa caaaatgcag ctgcgcttga ggtcgaactt tcgtcagcgg
     7321 aattagctat gctggataag gcatatccgg caccaaaagg aaaaactgcg ctggatatgg
     7381 tgtgatgttg atacgtgccg gatgcatcgc acccggcaca caacattaac gcaaattaac
     7441 gtttggcaac gcgaatggat tgcgccagat gtgcaggttt ctctttggtg actttttgcg
     7501 tttctgaagc gtaagccgtt tctacacaaa caaaggtctt gtagccatcg tccggcatgt
     7561 cgcccatgct aactgaaagc gccggtcccg ggttccagcc gacaacgttc agatgatgct
     7621 ggtggcctac ggcgataata cgattcagcg cttcatcatt aatcacgctg caatcttgcg
     7681 gattcagata cacgcgatcg gtacggtcag ggaaggtctg aataccgtcg gtcagtacat
     7741 cttctttcgc gtcattcact ttatcaatga agcgatcgcc cagcccactg acgcttacct
     7801 tagcgatatc acccacgtta aagtaggtat gcagggcaga ggtggtttca aattcgccat
     7861 gtgattcaag atcgatttca caggttttac ccacgcggaa atgcgctaac agcgtaaagt
     7921 cgtgcggcca gaattttttc gtctcttcgc tttgtgtcaa ttcaaaagtc agcgctacgc
     7981 catcagcatc ttcatgatgt gatttcagcg tccacggcag gttgcgggca aaaccgtgcg
     8041 caggcagacc ttgttgtgcc gccggaccaa accacggcca gcaaaccggt acgctaccgc
     8101 gaatagcgac gccatttttg aacggtgtgt tgttgctcaa ccacagaact tcttcttcac
     8161 ccgcaggttt ccacgagaga aggtgtgcgc cctgtaatgc aaaagaggct tttacctggg
     8221 gatgatcgac cacaatgagg tccagttcat ccagtttacg acgggagagg acaggggaga
     8281 tttgttcgat gaccggaagg gcaaaaattt tcttaatcat gacgcagtcc tttaacttca
     8341 ttttatcagg taaaaaaaag agcgaccgaa gtcgctcttt ttagatcaca gtgtcatctc
     8401 aacttatttg gagatgtgag cgatcaggtc cagaactttg ttggagtaac cggtttcgtt
     8461 gtcgtaccaa gataccagtt tcacgaagtt gtcgttcaga gcgataccag ctttagcatc
     8521 gaacacggaa gtgcaaactt cgccgttgaa atcggtagat actacgtcat cttcggtgta
     8581 gcccagaacg cctttcattt cgccttcagc agcagcttta acggcagctt tgatctgctc
     8641 gtaagttgca gctttttcca gacgaacggt caggtcaact acggatacgt tcggggtcgg
     8701 aacgcggaac gccataccag tcagtttgcc attcagttct ggcagtactt tacctacagc
     8761 tttagcagca ccggtagagg acgggatgat gttctgggaa gcgccgcggc cgccgcgcca
     8821 gtctttgtga gacgggccat caacggtttt ctgagtagcg gtagtagcgt gaacggtggt
     8881 catcagacct tcgatgatgc cgaagttatc gttgataact ttagccagcg gagccaggca
     8941 gttggtggtg caggaagcgt tggaaacgat gtcctggcca gcatatttgt cgaagttagc
     9001 gcctttaacg aacatcggag tgttgtcttt agacggacca gtcataacca ctttcttcgc
     9061 accagcggtg atgtgtttac gagcagtttc gtcagtcagg aacagaccag ttgcttcagc
     9121 gacaacgtca acacccactt cgtcccattt caggttagcc ggatcacgtt cagcggtaac
     9181 acggattttt ttaccgttaa cgatcagatg accgtctttc acttcaacgg taccgtcgaa
     9241 acggccgtga gtggagtcat atttcagcat gtatgccatg taatcagcgt ctaacaggtc
     9301 gttgattgca acgatctcga tgtcagaacg tttctgagca gcacggaaaa caatgcgacc
     9361 gatacggcca aaaccgttga tacctacttt gatagtcata tattccacca gctatttgtt
     9421 agtgaataaa aggttgcctg taaaattaca aaaaccttac gcagcgtcaa gcggaatcgt
     9481 gtcaatcatt gcgacagatc aatcctgtgc ctaagcatta cgcgactgac tcgcctcact
     9541 cttcctttgg gcttgagacc acatggggtc ggcgccccga attttaaagg gcaattacga
     9601 taaaaatgtg attttcatca cgatttaggt gcagccattt cgtcgagatc aagtttagga
     9661 caaaattttg ccagttggtg ttaatgtttt gttagaatca gtcacgtaat gtgagcacga
     9721 ttaaagtgag atgtgagtaa atggcgaata aaccttcggc agaagaactg aaaaaaaatt
     9781 tgtccgagat gcagttttac gtgacgcaga atcatgggac agaaccgcca tttacgggtc
     9841 gtttactgca taacaagcgt gacggcgtat atcactgttt gatctgcgat gccccgctgt
     9901 ttcattccga aaccaagtat gattccggct gtggctggcc cagtttctac gaaccggtga
     9961 gtgaagaatc cattcgttat atcaaagact tgtcacatgg aatgcagcgc atagaaattc
    10021 gttgcggtaa ctgtgatgcc catctggggc atgtctttcc cgacgggccg caaccaacag
    10081 gcgaacgtta ttgtgttaac tctgcttctt tacgcttcac tgatggcgaa aacggcgaag
    10141 aaatcaacgg ttgaaaaaac gattcagcaa cttattccac aggagcggaa aaattatgaa
    10201 tcttgatgac attatcaaca gcatgacgcc tgaggtatac cagcgtttgt cgaccgctgt
    10261 tgaactgggg aaatggcctg atggcgttgc gttgaccgag gaacaaaaag agaattgcct
    10321 gcaactggtg atgctgtggc aagcccgcca taataccgaa gcacagcata tgacaattga
    10381 cacaaatggt cagatggtca tgaagagcaa acagcagtta aaagaagatt ttggtatctc
    10441 agcaaagcct attgccatgt ttaagtaagt ggttcatccc ctttttgccg cggctatgaa
    10501 ggggatagcc aacaatgacc ccacgactcg tctcaattat ctcgacccga cctggctaag
    10561 tcccagtccg atctcttgca gtcgcgttca cgcctgaatg acttctcctg aacttaagtc
    10621 tcagacctat ttggccggta atccctctcg aatttgctct ttttgcgtcc cgccttccac
    10681 caacctgtaa cagacccagc cattttctgc gtaattgtga tctaagttgt aaaatgcagg
    10741 tcattatctg tgttgtgcag atttaatgtt gacctcaaaa atcatgtggg taagtatgat
    10801 ggccatctaa gcaaaacaaa acacagagga tgacgcaatg aaagcactgg ctcggtttgg
    10861 caaggccttt ggcggctaca agatgattca tgtcccacaa cccatttgtg gcccggaaga
    10921 cgtagtgatt gaaattaaag ccgcggcaat ctgcggcgca gacatgaagc actacaatgt
    10981 cgatagcggt tctgatgagt ttaactctat ccgcggccat gagttcgcag gttgtattgc
    11041 gcaggttggt gaaaaagtca aagactggaa agtggggcaa cgcgtcgtgt cggataacag
    11101 cggtcacgtt tgcggcgttt gtccggcctg tgaacagggt gattttctgt gttgtacaga
    11161 aaaagtaaac cttggtctgg ataacaatac ctggggcggt ggtttttcca aatattgtct
    11221 ggttcctggt gaaattctca aaattcatcg tcatgcgttg tgggaaatcc ctgatggtgt
    11281 tgattatgag gacgcagccg tacttgaccc tatctgtaat gcctataaat ccatcgcgca
    11341 gcaatcgaaa ttccttcctg gtcaggatgt tgtcgtcatt ggcactggcc cactcgggct
    11401 gttttccgta caaatggcgc ggattatggg ggcggtaaat atcgtcgtcg ttggtctgca
    11461 agaagatgtg gcggtccgct tcccggttgc aaaagaactg ggggcgacgg cagtagtaaa
    11521 tggttctacc gaagatgtgg tggcgcgctg ccagcaaatt tgtggcaaag ataatctggg
    11581 actggtgatt gaatgctccg gtgccaatat cgcactgaaa caagccatcg aaatgctccg
    11641 cccgaatggg gaagtggtac gcgttggaat gggcttcaaa cctcttgatt tctcgattaa
    11701 tgacattacc gcctggaata aaagcatcat tgggcatatg gcctatgact ccacctcatg
    11761 gcgtaacgct atccggctat tagccagcgg cgctatcaaa gtcaaaccga tgatcacgca
    11821 tcgtatcggc ctgtcgcaat ggcgcgaagg gtttgatgcg atggtcgata aaaccgcaat
    11881 caaagtgatc atgacttacg actttgatga ataactactc tcctgaagga acgttatcgc
    11941 atggaacaga taacaaaacc gcattgtggt gcacggctgg atcgcttacc ggattgccgc
    12001 tggcattcgt caatgtttgc catcgtcgcg tttggcttgc ttgtctgctg gagtaatgcc
    12061 gttggtggct tgatcctcgc gcagctgaaa gcgttgggct ggacagataa ttccaccaca
    12121 gccacattct cagcaatcac gaccgccgga atgtttctcg gtgcgctggt gggcggcatc
    12181 attggcgaca aaaccggtcg cagaaatgcg ttcatcctct atgaggccat tcatattgcc
    12241 tcgatggttg tcggtgcttt ctcaccgaat atggattttc ttattgcctg ccgttttgtg
    12301 atggacgttg ggctgggagc tttactggtt acactgtttg ctggtttcac cgaatatatg
    12361 cccggtagaa atcgcggaac gtggtccagt cgggtttcct ttattggcaa ctggtcatat
    12421 tcgctctgct cactgattgc gatgggactc acgccgctga ttagtgcaga gtggaactgg
    12481 cgggtacaac tgcttattcc tgcaatattg tcgcttatcg ctacggcgct ggcctggcgc
    12541 tactttcctg aatccccgcg ctggctggaa tcgcgcggac ggtatcagga agccgagaaa
    12601 gtcatgcgga gtatagaaga aggcgtcata cgccagacgg gtaaaccttt gccgcctgtg
    12661 gttattgctg atgacggtaa agcgccacaa gcggtgccgt attcagcctt actgacagga
    12721 gtattactga aacgcgtgat attaggctct tgtgtgctga ttaccatgaa cgttgtgcag
    12781 tacacactaa ttaactggtt gccaacaata ttcatgaccc aggggattaa tttaaaagac
    12841 tcgattgttt taaataccat gagtatgttt ggcgcgccat ttggtatttt tattgccatg
    12901 ctggtgatgg ataaaattcc gcgtaaaaca atgggtgtgg ggctattaat cctgattgcg
    12961 gtgctcggat atatctattc actgcaaacc agtatgttgc tcataacgct gattggtttc
    13021 ttcctgatta ctttcgtcta tatgtacgtt tgctatgcct cggcagtgta tgtccctgaa
    13081 atctggccga cagaggccaa actccgtggc tccggtctgg cgaatgcggt agggcgaatc
    13141 agtggtattg ccgcacctta tgccgttgca gtgctgctca gtagttatgg tgtaacggga
    13201 gtctttattc ttctgggggc tgtttcaatt attgtcgcaa ttgctatcgc caccattgga
    13261 attgaaacca aaggtgtctc cgttgaaagt ttaagtattg atgcagtagc caataaataa
    13321 tacgggtgga ttcaaaatga aaaattcaaa agcaatattg caggtgccgg gcacaatgaa
    13381 aattatttca gcagaaatac cagtgcctaa agaagatgaa gttttgatta aagtagaata
    13441 tgtcggtatt tgtggttcag atgtacatgg ttttgaatca ggcccgttta ttccgcctaa
    13501 agacccaaat caagaaattg gcctgggtca tgaatgcgcc gggacggttg tggctgtggg
    13561 aagccgcgtg cgcaaattta aaccggggga tcgggtaaat atcgaacctg gcgttccttg
    13621 cggtcactgt cgttactgtc tggaaggcaa atataacatc tgcccggacg ttgattttat
    13681 ggcgacacaa cccaactacc gcggcgcatt aacgcactat ctgtgtcatc cggagagctt
    13741 tacttacaaa ctgcccgaca atatggacac gatggaaggg gcgctggtgg agcctgccgc
    13801 agtcgggatg catgccgcga tgctggcaga tgttaaaccg ggtaagaaga taattattct
    13861 gggagcaggt tgtattggtt tgatgacgtt gcaagcgtgc aaatgcctgg gcgcaacgga
    13921 aattgccgtc gttgatgtac tggaaaaacg tctggcaatg ggggaacagc ttggtgcgac
    13981 agtggttatt aatggcgcaa aagaggacac tattgcacgc tgtcagcaat ttaccgaaga
    14041 catgggcgca gatattgttt tcgaaacagc gggttctgcg gtcaccgtta aacaggcacc
    14101 tttaatctgg taatgcgcgg cggtaaaatt atgattgttg gtactgtacc cggcgattcg
    14161 gcaatcaatt tcctcaaaat caatcgcgaa gtcactatcc agacggtatt ccgctatgcc
    14221 aatcgttatc cggtcacgat tgaagctatt tcttcagggc gattcgatgt gaaatcgatg
    14281 gtgacgcata tttacgatta tcgggatgta caacaggcat ttgaagagtc agttaacaac
    14341 aagcgcgaca ttattaaagg cgttattaaa attagcgatt aattaatacg ggagaacaga
    14401 acatgctcgc agatatcaga tattgggaaa acgatgccac aaataaacat tatgcaattg
    14461 cccattttaa cgtatggaat gcagaaatgt tgatgggcgt taaagacgca gccgaagaag
    14521 cgaaatcccc ggttattatt tcttttggta caggttttgt cggtaacacc tcatttgaag
    14581 atttctctca catgatggta tcaatggcac aaaaagcaac ggtgccggta ataactcatt
    14641 gggatcatgg tcggagtatg gagattattc ataacgcctg gactcatggt atgaattcat
    14701 taatgcgtga tgcttctgca tttgatttcg aagaaaatat tcgtttaacc aaagaggctg
    14761 ttgatttctt tcatccgctg ggtattccgg tagaggcgga attagggcat gtcggtaatg
    14821 aaaccgttta tgaggaagcg ctggcgggct atcactatac cgatcctgac caggcggctg
    14881 aatttgttga acgtacgggc tgtgactcat tggctgtcgc catcggcaac cagcatgggg
    14941 tttatacgtc agagccgcaa ttgaactttg aggtcgtcaa acgcgtacgc gatgctgttt
    15001 ctgttccgct ggttttgcat ggcgcatcgg ggatcagtga tgccgacatt aaaactgcaa
    15061 tttcgttggg tattgcgaaa atcaacattc atacggagct ctgtcaggcc gcaatggtcg
    15121 cagtgaaaga aaaccaggat cagcctttcc tgcatttaga acgtgaagta cgtaaagctg
    15181 taaaagaacg ggcattggaa aaaattgaac tgttcggttc tgatggcaaa gcggaataat
    15241 gatatggata atctcgacgt tatttgtata ggtgccgcta ttgttgatat tccattacaa
    15301 ccggtcagta aaaatatctt tgatgtagat tcttatcctc ttgaaagaat cgcaatgagc
    15361 accggggggc gatgcaataa atgaagcaac aattatttct cgtctggggc atcgtaccgc
    15421 attaatgagc cgcattggta aagatgcctc agggcaattt attctcggcc attgtcgcaa
    15481 agagaatatt gatattcaaa gcctgaaaca ggacgtcagc atagatacat ctattaacgt
    15541 tggactggtg acggaggatg gcgagcggac gtttgtcacg aaccgtaacg gcagtttgtg
    15601 gaaattaaat attgacgatg ttgattttgc gcggtttcct caggcgaaat tattatcact
    15661 ggccagtatt ttcaacagtc ctctattgga tggtaaagcg ctaacagaaa tttttacgca
    15721 agcgaaagcc cggcagatga tcatttgtgc cgatatgatc aaaccgcggt tgaatgaaac
    15781 gctggatgat atttgcgaag cattgagtta tgtcgattat ctgtttccaa attttgccga
    15841 ggcaaaatta ctcacaggga aagagacact ggatgaaatt gctgactgct ttcttgcgtg
    15901 cggcgtaaaa acggtggtga ttaaaacggg taaagacggc tgctttatca agcgtggtga
    15961 catgacgatg aaggtgccgg cggtcgcagg aataaccgcc atcgacacca ttggcgcggg
    16021 cgataacttt gcttcaggtt ttattgcggc actgttagaa ggcaaaaatc tgcgtgaatg
    16081 cgcacgcttt gccaatgcaa cggcggctat ctcggttcta agcgtcggtg ccaccaccgg
    16141 cgtaaaaaac agaaagctgg tcgaacaatt gctggaagaa tacgaaggat aatgaaggca
    16201 aatgaaaaag atacctttag gcacaacgga tataacgctt tcgcgaatgg ggttggggac
    16261 atgggccatt ggcggcggtc ctgcatggaa tggcgatctc gatcggcaaa tatgtattga
    16321 tacgattctt gaagcccatc gttgtggcat taatctgatt gatactgcgc taggatataa
    16381 ctttggcaat agtgaagtta tcgtcggtca ggcgttaaaa aaactgcccc gtgaacaggt
    16441 tgtagtagaa accaaatgcg gcattgtctg ggaacgaaaa ggaagtttat tcaacaaagt
    16501 tggcgatcgg cagttgtata aaaacctttc cccggaatct atccgcgaag aggtagaagc
    16561 cagcttgcaa cgtctgggta ttgattacat cgatatctat atgacgcact ggcagtcggt
    16621 gccgccattt tttacgccga tagctgaaac tgtcgcagtg cttaatgagt taaaggctga
    16681 agggaaaatt cgcgctatag gcgctgctaa cgtcgatgct gaccatatcc gcgagtatct
    16741 gcaatatggt gaactggata ttattcaggc gaaatacagt attctcgacc gggcaatgga
    16801 aaacgaactg ctgccgctat gtcgtgataa tggcattgtg gttcaggttt attccccgct
    16861 agagcaggga ttgttgaccg gcaccatcac tcgtgattac gttccgggcg gcgctcgggc
    16921 aaataaagtc tggttccagc gtgaaaacat gctgaaagtg attgatatgc ttgaacagtg
    16981 gcagccactt tgtgctcgtt atcagtgcac aattcccact ctggcactgg cgtggatatt
    17041 aaaacagagt gatttaatct ccattcttag tggggctact gcaccggaac aggtacgcga
    17101 aaatgtcgcg gcactgaata tcaacttatc ggatgcagac gcaacattga tgagggaaat
    17161 ggcagaggcc ctggagcgtt aaatactcaa cacgattcac ctctgtttct gtgaaaactg
    17221 tgatgaaaat ctgtgattca aataggttat tttgaaagta aacatcggtt atgcgataat
    17281 cgcgctaatg tgatgtaaaa agatacaggg gtgtaatcgt ggcggcaaaa gacaggattc
    17341 aggcaattaa gcaaatggtt gccaacgata aaaaggtgac agtctcaaat ttgagtggga
    17401 tttttcaggt aaccgaagaa accattcgcc gcgatcttga gaagctggaa gatgaaggct
    17461 ttttgaccag aacctatggt ggtgctgttt taaatacagc ggtgttgacg gagaatatcc
    17521 atttttataa gcgcgcttca tcgttttatg aagagaagca gcttattgca cgcaaggcac
    17581 taccctttat cgacaataaa accaccatgg cagccgattc cagtagtacc gtaatggaat
    17641 tgctcaaatt gttacaggac cgtagtgacc tgacgttgct aaccaactcg gcggaagcga
    17701 ttcatgttct ggctcagtca gaaattaaag tcgtttcaac gggtggggaa ctaaacaaaa
    17761 atacactttc cctgcaagga agaattacta aagagatcat cagccgctac catgtcgata
    17821 tcatggtaat gagttgtaaa ggtcttgata ttaacagcgg cgcgctggac tctaatgaag
    17881 cggaagctga aatcaaaaag acaatgatcc gtcaggcgac agaagttgcg ttattggttg
    17941 atcactctaa gtttgatcgt aaagcttttg tccagttagc tgactttagt catattaatt
    18001 atataataac tgataaatca ccgggtgcag agtggatagc attttgcaaa gacaataata
    18061 tccagctggt gtggtaattt aagcacacgt tgtcaatatt atttgttcct gcgcctggaa
    18121 ataaaatgat gataaagatg cggcaagtga atttgccgca attgctacct tttatagcaa
    18181 atccgaggca cccaatggaa caatatgatc aaattggcgc aagactggac cgcttgcctt
    18241 tggcccggtt tcattatcgt atatttggta taataagctt tagtctgtta ttaacggggt
    18301 ttttgagtta ctcaggtaat gtcgtcttag caaagctggt aagcaatgga tggtcaaata
    18361 atttcctcaa tgccgccttt acctcggcat taatgtttgg ttatttcatc ggctcactta
    18421 ctggtgggtt tattggtgac tactttgggc ggcgcagggc gtttcgcata aatcttctca
    18481 tcgtcggtat tgctgcaaca ggggccgctt ttgtccctga tatgtactgg ctcattttct
    18541 tgcgcttcct gatgggaaca ggaatggggg cgctgattat ggttggctat gcctcattta
    18601 cggagtttat ccccgcgacg gtgcgtggaa aatggtccgc gcggctctca tttgttggta
    18661 actggtcgcc catgctgtct gcggcgatag gcgtggtggt tatcgctttt tttagttggc
    18721 gaataatgtt tcttctgggt ggtattggca tactgttagc ctggtttctc tcaggtaaat
    18781 actttatcga gtcgccacga tggctggcag ggaaagggca aatcgccggt gcagaaagcc
    18841 aacttcgtga agtagagcag caaattgaaa gggagaagag tattcgttta ccccagctta
    18901 ctttgaacca gagcaacagc aaggttaagg taatcaaggg taccttcagg ctcctgttta
    18961 aaggggaaat gttacgacgt acattagttg cgataactgt tttaattgca atgaacattt
    19021 cgctttatac cattaccgta tggataccga ccatatttgt taactccggc attgatgtcg
    19081 ataaatcaat attaatgacc gctgttatta tgattggtgc tccggtagga atatttattg
    19141 cggcattaat tattgatcat tttcctcgtc gattatttgg ctccgcctta cttattatta
    19201 ttgccgtgtt aggctatatc tattcaagtc agactacaga gtgggcgatt ttaatctatg
    19261 gtctggtgat gatcttcttt ttatacatgt atgtttgctt cgcgtcggcg gtttatatcc
    19321 cggagctttg gccaacgcat ttacgcctgc gcggttcggg tttcgttaat gccgtcggac
    19381 ggatcgtcgc agttttcacg ccctatggcg ttgcggcatt attaacacat tatgggtcga
    19441 tcacggtgtt tatggtgctt ggtgtcatgt tagtgctctg tgcgctggtt ctctccattt
    19501 ttggcatcga aacgcggaag gtgtcgctgg aagagatttc tgaggtgaat taaatctttc
    19561 gttgatggca tcaattgcca gatgtaccac tgacgtgttt tatttggcct acaatgccag
    19621 tacagaattg taggccagcg taaaattacc cctgcgtctc ttcccagtct gccagcgtat
    19681 atagcgttgc cccagctgct gacatctcca taaacgcgtg tgcactgtcc tggggctgga
    19741 tattcacgcc acgacagcca tcggtaatca cgtttacctt ataacctaac tgtaacgcgt
    19801 ccagcacggt aaacttcacg caatagtcag tagccaggcc cataacgatc aattcatcga
    19861 tttcatgatc gcgcaaccag tcatcaagcg cggttttctg ccgacgtccg ttatcaaaaa
    19921 aggcactgta actgtcaact aaaggatttt cgcctttatg gaacaccgct gcgatctctt
    19981 tttgattcag taacggatgt aattgtgcgc cttcactgtt ctgcacacag tgttcaggcc
    20041 agaaagtttg ccgcaggccg tcgagttggc ctggcgtata aggctctacg ccgtgctggc
    20101 tggcaaagct gccgtgattc gccggatgcc agtcctggct ggcgataacc gcttctccgc
    20161 gcgactggca ccagtcaata aggcggttag caacctccac cgtactgtca ccttccggta
    20221 cggcgagcgc gccaccagca cagaaatcat tttgtaaatc gaccagtaac agggcgcgat
    20281 ggggcattgc agtctcctta atcatccagc gtcagttcgc cgcgcaggtt ttggctcatg
    20341 gccttgcgaa tggtttcagt atccagttcc tggctcagca ggtaatgcag tttggttagc
    20401 gtggcttcta cagtcatatc tgcaccgcca ataacgccgg catgggcgag gacgttaccg
    20461 gtggcataac cactcatatt cactttaccg gacatacatt gtgtcaggtt gaccaccaca
    20521 ataccgcgat cgctggcttc ttgtaattcc tgcaggaagg ctttgttttg tggcgcatta
    20581 cccacgccat aggagcgcag aatcaatgct ttcaccggtt ggcgcagaaa attgcgcacg
    20641 acgtcagcag aaatccctgg ataaatcgtc actacgccaa ttggttgtgg cgtgattgga
    20701 tgaacgatta attccccttc accgtgcggg gcgggtggcg tattcaaacg acgaatatgg
    20761 ataccagctt ccagtaacgg aggaaggttt ggagaggcaa acgcatcaaa accatcggca
    20821 tgggctttgg tagtgcggtt gccgcgatac aatcggttat tgaaaaagag cgttacttcg
    20881 ttgatcggat aattcgccgc aacgtacaac gcattcagca gattaatttg tccgtcagag
    20941 cgtaactcag ccagcgggat ttgtgaccct gtcacaatga ccggtttacc gagattctcg
    21001 agcatgaacg acagcgcaga ggcggtatac gccatcgtgt cggtaccgtg tagaatgaca
    21061 aaaccatcat agtcgtcata gtgcgcttta atatcttcag caatatgctg ccagtcttcc
    21121 ggcgtcatat cagaagaatc catcaacggc gtatattcat gaatggtgaa atctggcatc
    21181 tccaggcgat ggaattccgg catcagcgcc agttggcgtt gtagatgacc tgacaccgga
    21241 atataaccct gctcggaacg ctgcatcccg atggtcccgc ccgtgtaggc gacgtaaatt
    21301 gatttctttt gcatgatatt gatatgaatg atatcgaaag agcaaaagta tatacgtgag
    21361 gcaggaggag cgcacctgat actatgcgga taaggaaata tggggcgaga cagcgggaag
    21421 aggattcgtc gaggtaaaaa ataatgtggc cgacgatctg tcggccactc agtacaagac
    21481 ttaacgcacg ttggcgcagg tcaggcaaaa cgcataacgg ttttgtgggt cgttaaacgc
    21541 ggccagcttg tcgctttcac ttttaacggt agaggctacc gaggcaagcg gtgcaggtaa
    21601 catggcctgg aacgcatctg gcaacattgc ccggacagaa ccagacatgt tgtccatcac
    21661 tttgtcgaag aaggtcggtt catcaacgta gtattccaga tgccactgtt tcacttttgc
    21721 gagctctgct gctttggcaa ccgcatcatc gaaatccccg agactatcga ccagcccgtt
    21781 agcttttgca tcctgaccgg tccagacgtg gccctgggcg attttatcaa tttgctccgg
    21841 cgtcgaatga cgcgcatcag caaccagcgt gataaagcgt ttatagccat tctcaatgct
    21901 taattgcatc atctgctgcg cttccggcgg cagtgccctg gtgatagaaa catccgccag
    21961 cggtgaagtt gagacaccat cagtatgaac accaatcgaa tccagactat tttctacggt
    22021 ggtgatcacg ccgaagatac cgatagaacc ggtcagggtg ctggggttag ccacaatgta
    22081 attagctggc gtggaaatcc agtaaccacc agatgccgcc atgccgccca tcgatacaac
    22141 cacagactta cccgctgccc gggctgctgc cagttcagcg cgaatcactt cagacgcggt
    22201 aacgctgccg cctgggctat taacacgcag gacaatcgct ttcactttcg ggtcaaggcg
    22261 agcgtcgcgg atttgtgccg cagtggtatc accgccaaca ttcccctgag tttcctcgcc
    22321 atccataatt gcgccattag cgaagacgac accgatgctg tcaccggtat ctgccggcgt
    22381 tttcaatgcg taatcgtaat aactgatggc gcgataattt ttatcagtct tactccagcc
    22441 gaactctttg gtcagagctt tttcgatttc ggcactcgat gccagtgcat cgaccagctt
    22501 gttttccagt gcatatttcg cggtatcgcc accggttttg gttaaaccct caagcaaccc
    22561 ttgcgcgcca gggaatacct gctgagcagg gatctgccgg ttagcggcaa cagtattcag
    22621 atagttttgc cacagctcac caatccagcg gctgtcagct tcgcgggctg ccggtgacat
    22681 atcatcacga ataaacggtt caacggcaga tttatacgta cccacgcgga acacatgggt
    22741 ggaaactttc agcttatcca gcaacgattt gtagtacaga ccgttggtag caaaaccgtg
    22801 cagatcaacc acgccttgcg gtgacagcca aattttattg gcgaaactgg cgagataata
    22861 ttgcccctgg ctgtagttct cgccaacggc ataaaccggt ttcccgctgt cacgaaactc
    22921 tttcagggct ttgccgatgt actgcataga cggctggtcg ccgcctgcga agtttttcag
    22981 atccatcacg ataccggtga tattgcggtc gtccttcgcc tggcgaatgg tgttgacgat
    23041 atcaaacagt gagttttcct gcagacgatc ggaactggca ccaagcagct ggcggcttaa
    23101 tttactaaac cgctgagaac tgtctggttt atcgacgatc acaccagaaa tgtccagcag
    23161 cagtgcgcca cgactggccg tttctttcga atcaccacca ctgacctgca tccagatccc
    23221 cacaccaacc agtacgagga aaataaagaa caggttaagt accatttcac ggacgaaatt
    23281 cagcagacgc cacgtccatt taaaaaatcc ggcaataaat cgccaaaggg ttcgcatgta
    23341 ttctcccaac ttaaggtcac acctgtcaca ggcgcaatag atgtggttat cgtaatgacc
    23401 caaccggcac ttgtcagcag gaattgcgga ctgtgctgta acaaaatcca ctctcgtgtt
    23461 aattttgtga ataaatatca cgacaggagt taatcaaatg gatgcactcg aactattgat
    23521 caatcgccgt agcgcctccc gcttggctga acccgcgcca acgggtgaac aactgcaaaa
    23581 catcctgcgt gcgggtatgc gtgcgccgga ccataagtcc atgcaaccgt ggcatttttt
    23641 tgtgattgaa ggggaagggt gcgagcgttt cagcgccgta ctggaacagg gggcgattgc
    23701 tgccggtagt gatgataaag ctatcgacaa agcccgtaat gcgccgttcc gcgcaccgct
    23761 catcatcacg gtggtggcga aatgcaaaga aaatcataaa gtcccgcgat gggaacagga
    23821 aatgtctgcc ggatgcgcgg tcatggcgat gcaaatggca gccgttgccc agggatttgg
    23881 cggcatctgg cgcagtggcg cattaactga aagtccggta gtgcgtgaag cattcggttg
    23941 ccgtgagcag gataaaattg tcggttttct ctacctcggc acgccgcagt tgaaagcatc
    24001 tacgtcgatt aacgtcccgg acccgacgcc gtttgtaact tatttctgat aattgtcgcg
    24061 aaactgtctg gattgtgagc aaaggcgcag gaattcagac actctcactt atcacttcac
    24121 ggaatgaggg ttaccatacc cggattgcat tgacaggaga tgtccatgag cgagaactcg
    24181 attcgtttga cccaatacag ccacggagct ggttgcggct gtaaaatttc cccaaaagtg
    24241 ttggaaacca tcctgcacag tgagcaggcg aagtttgttg atccgaattt gcttgtgggt
    24301 aatgaaaccc gcgacgatgc ggcggtgtac gatctgggca atggcaccag cgttatcagt
    24361 accaccgact tctttatgcc gatcgttgat aatcctttcg attttggccg cattgcggcg
    24421 actaacgcca tcagcgatat cttcgcgatg gggggcaaac cgattatggc gattgcgatc
    24481 ctcggctggc cgattaacaa actttccccg gaaattgccc gcgaagtaac cgaaggtgga
    24541 cgctatgcat gccgtcaggc gggtattgcg ctggctggcg gtcactccat cgatgcgccg
    24601 gagccgattt ttggtctggc ggtaacgggg atcgtaccga ccgagcgggt gaagaaaaac
    24661 agtaccgcac aagccggatg caaactgttc ctgacgaaac cgctggggat cggcgttctt
    24721 accacggctg agaaaaaatc actgttgaaa ccagaacatc agggactggc gacggaagtg
    24781 atgtgccgga tgaacatcgc aggcgcgtcc tttgccaaca tcgaaggcgt aaaagcgatg
    24841 accgacgtta cgggctttgg tctgctgggc cacttgagcg aaatgtgtca gggggctggt
    24901 gtgcaggcac gcgtcgacta tgaagcgatc ccgaaactcc ccggtgttga agagtacatt
    24961 aagttgggcg cagtacctgg cggcactgaa cgtaactttg ccagctacgg tcatctgatg
    25021 ggtgaaatgc cgcgtgaagt gcgcgatctg ctgtgtgatc cgcaaacttc tggcggtttg
    25081 ctgctggcgg tcatgccgga agcagaaaat gaggttaaaa ctacagccgc cgagtttggc
    25141 attgaactga cggcgattgg cgaactggtg ccagcgcgcg gcggtcgtgc catggttgag
    25201 atccgttaat tcaatgcggt tgtttattgc cgaaaaaccg agtctggcgc gcgccattgc
    25261 tgatgtcctg cccaaaccgc accggaaagg cgatggcttt atcgagtgcg gtaatggtca
    25321 ggtggtgact tggtgtatcg gtcacctgct tgagcaggcg cagccagacg cctacgacag
    25381 ccgctatgcg cgctggaatc ttgcggattt gccgattgtc ccggaaaagt ggcaattaca
    25441 gccacgaccc tccgtgacca aacaacttaa cgtcatcaaa cgcttcctgc atgaagccag
    25501 cgaaatcgtt cacgccgggg acccggatcg tgaagggcaa ttgctggtgg atgaagtgct
    25561 ggactatctg caactggcac cggaaaagcg ccagcaggtg cagcgttgct tgataaacga
    25621 cctgaacccg caggcggttg agcgggcgat cgaccgtctt cgttccaaca gtgagtttgt
    25681 gccattgtgc gtttctgcgc tggcgcgagc gcgtgccgac tggctgtacg gcatcaatat
    25741 gacccgtgcg tataccattc tcggtcgcaa tgccggttat cagggcgtac tttccgtggg
    25801 acgcgtgcag acacccgtgc tcgggctggt ggtgcgccgc gatgaagaga ttgaaaactt
    25861 cgtggcgaaa gacttctttg aagtcaaagc acatatcgtg acacctgccg atgagcggtt
    25921 taccgctatc tggcaaccga gcgaagcgtg tgaaccgtac caggatgaag aagggcgctt
    25981 gttacatcgt ccactggcgg agcatgtggt taaccgcatt agtggtcaac cggctattgt
    26041 caccagctat aacgataaac gggaatcaga atccgcgccg ctgccgtttt cgctttcggc
    26101 gttgcagatt gaagcggcaa aacgcttcgg tctgagcgcg cagaacgtgc ttgatatctg
    26161 ccagaagctg tacgaaacgc acaagctaat cacttatccg cgttccgatt gtcgctattt
    26221 gccagaagaa cattttgccg gacgccacgc ggtgatgaat gccatcagcg ttcatgcacc
    26281 ggatctgttg ccgcagccag tggtagatcc agatatacgc aaccgctgtt gggatgacaa
    26341 aaaggtcgat gcgcaccacg ccatcatccc gaccgcacgg agttctgcga tcaacctgac
    26401 ggagaacgaa gcgaaggtct ataacctgat tgcccgtcaa tacctgatgc agttctgccc
    26461 ggatgcggtg ttccgcaagt gtgttatcga actggacatt gccaaaggca aatttgtcgc
    26521 taaagcccgt tttcttgctg aagcaggctg gcgcacgctg ttaggcagca aagagcgcga
    26581 tgaagaaaac gacggcacgc cattgcctgt ggtggcgaaa ggcgatgagt tgctgtgtga
    26641 aaaaggtgaa gtggtagagc ggcaaaccca gccgccgcgc cattttaccg atgcaacact
    26701 gctttcggcg atgaccggga tcgcgcgctt tgtgcaggac aaagatctga aaaaggtcct
    26761 tcgtgcgacc gatggtctgg ggacagaagc aacccgtgcc gggattattg aactgctgtt
    26821 caagcgtggt tttctgacca aaaaagggcg ctatatccac tccaccgacg ccggaaaagc
    26881 gttattccat tcgctgccag aaatggcgac gcgaccggac atgaccgcgc actgggaatc
    26941 ggtgctgacg caaatcagcg aaaagcagtg tcgctatcag gactttatgc agccgctggt
    27001 ggggacgcta tatcagctta ttgatcaagc caaacgtacg ccggtgcggc agtttcgcgg
    27061 cattgtggct ccgggcagtg gtggcagtgc tgataagaaa aaggctgcac cgcgtaaacg
    27121 tagtgcgaaa aaaagtccgc cagcagatga agccggaagc ggggcgatag cgtaagcgag
    27181 cgaatctttc gtgctattcg agtcatattc tgaaatatcc agcggatcaa gaaaattcgt
    27241 tggatatttt tttgcatgga taaaattatc gcctctaaag tatgtaataa cagggaatgt
    27301 gatgaaaaaa gttttattac aaaaccatcc tgggagcgag aagtattctt ttaatggctg
    27361 gggaatattt aatagtaatt ttgaacgggt gattaaagaa aataaggcca tgctgctttg
    27421 taagtggggg ttttatttaa catgtgttgt cgctgtaatg tttgtattcg cagcgataac
    27481 atccaacggt ttgaatgaaa gaggcctgat taccgcggga tgctcttttc tttatctatt
    27541 aattatgatg gggcttattg ttcgggccgg ttttaaagca aaaaaagaac aactacatta
    27601 ttatcaggct aaaggtattg agccgctcag tatcgagaag ttacaggcgc tacaattgat
    27661 cgcaccttat cgattctatc ataagcaatg gtctgaaacg ctagagttct ggccgcgaaa
    27721 gcctgaacct ggcaaagata ccttccaata tcatgtgctt ccttttgact cgatcgatat
    27781 catcagtaaa agacgcgagt ctttagagaa tcaattgggt atcgaagata gcgaaagtta
    27841 ttgtgcctta atggagcatt ttctttctgg cgaccatgga gccaatacct ttaaagcaaa
    27901 catggaggaa gccccagagc aggttatcgc cgtgttgaat aaatttgctg tttttccctc
    27961 agactatatc tctgattgcg ctaataatcg ctccggtaaa tcctcggcga agctaatatg
    28021 ggcggcggaa ttatcatgga tgatctcgat atcaagcaca gcttttcaaa acgggacaat
    28081 tgaagaagaa ctggcctggc attatataat gcttgcttct cgaaaggcgc acgagttgtt
    28141 cgaaagcgaa gaagattatc aaaaaaatag tctaatggga tttctttact ggcatatctg
    28201 ctgctatcgc agaaagttaa cggatgctga acttgaagcg tgttatcgtt acgacaagca
    28261 gttttgggag cactacagta agaaatgccg ttggcccata agaaatgttc cctggggagc
    28321 atcatccgtt aaatactcat aaacgcctga aattttgccg ggggcgctgc gcttgcccgg
    28381 cctacattag agcgtaactg caatgaattg tgcccatctg taggcctgat aagcgtagcg
    28441 ccatcaggca tttacaactt aaatcacacc ctgcgccagc atcgcatcgg caaccttcac
    28501 aaaaccggca atgttcgcgc cctgcacgta gttggtttgc tcaccttcac caccatgctc
    28561 aacacaggca tggtggatat ccagcatgat gtgatgcaaa cgtgcgtcaa ctttctcggc
    28621 tttccagccc aggcgcgcag cgttttgtgc catttccagg cccgacgttg ctacaccgcc
    28681 agcattagcc gctttacccg gtgcaaacag tacgcctgcc tgctggaaca gttcagtcgc
    28741 ttcgatggtg gtcggcatat ttgccccttc agcgacggct ttaacgccat tggcgataag
    28801 ctgatgcgcg gtgtcaacat ccagttcgtt ctgggtggcg caaggcaggg cgatatcaac
    28861 cggtacagac cacggctgtt ggccttcgag atagaccaga ccaaattctt tggcgtaatc
    28921 tgccactcga ccatcgcggc tggctttgat ttcgataaga cgtgccagtt tctctttcgt
    28981 gaatccgctt tcatcaacta cagtgccgct ggagtctgac gcagtgatca cacgagcacc
    29041 aaattccatc gctttttcga tagcgtactg ggcgacgttg ccggagccag aaacggaaac
    29101 gcgcatccct tcaaaaccca taccgtggcg ttttaacatt gcttctgtga aataaaccag
    29161 accgtagccg gtagcttccg ggcgaataag actaccgcca aatgaaaggc ccttaccggt
    29221 gaagacgcag gcggtattgt tggagagctt tttcatcatc cccgccataa agccgacttc
    29281 acgaccacca acgccgatat cacctgccgg aacgtcggta tccgcgccca ggtggcgata
    29341 cagttcagtc atcagcgcct ggcaaaaacg catcacttta ccttcgcttt ttcctttcgg
    29401 atcgaaatcg ctgccgcctt taccaccgcc catcggcagg gtagtcaggg catttttgaa
    29461 ggtttgttca aagccgagga atttgagaat ggaaaggtta actgacggat ggaagcgcat
    29521 accgcctttg tacgggccga tggcagagct gaactgcaca cgccatgcac ggttgacctg
    29581 tatctggttg cgatcatcaa cccataccac gcgaaactgg atcacgcgct ccggttcaac
    29641 cagacgctcc agtaatgaca tctggcgata ttttggattt tgttcaagaa aaggccagag
    29701 tgtggtcatt acttcacgaa cggcttgcgc gaactcggtt tgattcgggt cgcgcttttg
    29761 gacatggttg aggaatgact ccagagaata tgtctgatcc atagatataa aaaccttata
    29821 tattaatacg attgtgctta tgttgtttat gtcatgtgct tttgcagttt tcgactatac
    29881 catcaagaat gtgacggtaa gcaagaaaaa aatgacccag gaaagcaaat taataacgag
    29941 agtaatctca taaccaaatg cgataactca gacgctaaag ttttgccacc cggctaccgt
    30001 tatcagtagc agagacaata acgttaagga gttccctgtg agtcgagcat tgttcgccgt
    30061 agtattagca tttccattaa ttgcactggc taacccgcat tatcgtccgg acgtggaggt
    30121 aaacgtaccg ccagaagtgt tcagttcagg cgggcagtca gcgcagccct gtactcagtg
    30181 ctgcgtttat caggatcaaa attattctga aggggcggta attaaagccg aaggaatact
    30241 gctgcaatgc cagcgtgatg acaaaaccct tagcactaat ccgctgctct ggcggcgcgt
    30301 aaagccataa acgcctctaa taatggaatg tcagcagggg ccagcggata ttgcagcgcc
    30361 tcttcaggtg agcaccagac cagcgcctga tgttcatgtg cctgtaacgt cccgtggaag
    30421 tcgggtacgt gccaggcatg aagatggata atccggcccg aaacttctcg ctgatggctg
    30481 gcaacatact cacccacagt tgcttcgatg tccaattctt cgtttaactc acgcaccagc
    30541 gcctgctgct ggctttcatc cagctcgact ttacctccgg caaactccca taatcccgcc
    30601 tgatcgctct gggcggggcg ttgcgcgagt aaaattttgc catcacgttc aatgatggcg
    30661 gcaacaactt caatcatttt cataaattac acacgggcag ataagcgaaa aatgccataa
    30721 tatccgcact gttgctcaat cagaaaggct ttatatttaa ggagtgcctg tgctagaccg
    30781 ccatcttcat ccccggatta aaccgttgtt gcatcagtgc gtgcgggttc ttgataaatc
    30841 gggcattacg ccagatggtt taacattagt gggatttgcc atcggcgtgc tggctctgcc
    30901 gtttctggcg ctgggctggt atctggcggc gctagtcgtc attttgttga acaggctgct
    30961 tgatggtctg gacggtgcgc tggcgcggcg cagagggctt accgacgcgg gcggctttct
    31021 tgatatttct ctcgattttc tcttttacgc gctggtgccg tttggcttta ttcttgctgc
    31081 accggagcaa aatgcgctgg caggcggctg gttgttgttt gcgtttatcg gcacgggcag
    31141 cagttttctc gcctttgctg cactggcagc aaaacatcag attgataacc ccggttatgc
    31201 acataagtcg ttttactatc tggggggatt aaccgaaggc acagaaacga tcttactgtt
    31261 tgtgctggga tgtttattcc ctgcatggtt tgcgtggttt gcgtggtttg cgtggtttgc
    31321 gtggtttgcg tggtttgcgt ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac
    31381 ctttacgcgg gtgtggagtg ggtatctgac gctgaagtcg ctccagcgtc agtaattgca
    31441 atgttatttg ctactgcccg ggccgcgttc accggttgca accggatttt ttggatcgct
    31501 gctccattcg taccagccgc cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc
    31561 gtacataaag gtttcggacg cgcgccagcc ggtgccgcag tagaatgaaa cttgctgatc
    31621 tggtttgata ttccatgctt tccacatagc ggtaatatca tcggcgctac gcatggtgcc
    31681 atccgggtta tggaaatctt ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc
    31741 tccggctatt tcaccttttg gtttaatata gctgtaaccg ctggtcgtac cgataaattc
    31801 tggccacgaa cgaatgctca ccagcgatgc atcctggcga tgcagcagtc cacgggcctg
    31861 ttccatatcg agcatcaact gcggttgtgc cgggatcttc acgccgaaat ctggttccgc
    31921 tttcactttc ggtggcgttc ctcgctcaac aggcagtccc gcgtcggacc aggtttgcca
    31981 gccgccatcc agcagacgca catctttcac gcctgcgtag agcataattt gcgccacacg
    32041 cgctccagcg tatacgtcac gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg
    32101 ttttgccagc atcgctttta gttgttcatc agaaactttg ttccacagcg gttcgctttc
    32161 cacttcgttg gtatcgatgt agtcagcgcc aggaatatgg ctgataaggt aaagcttagg
    32221 agcgccccag gccgcttcaa tgactttcca gccaccgaca ggtttcgccg taacctcttt
    32281 accttgttgc aggtcatgca gccattgcgg ataaaccagc tgctcaaagt gcggcagttt
    32341 ttgtagacgg gaaggttcgc ttagcgcgtc actcagaatg gagatatgcg taaaacctgc
    32401 tttttgcagt cgcgttttga cggcgtcgac atctttgcca ttaccgtaca gcgccaccgg
    32461 ggcatcggct ttcaggttat gttgcttgat ccacgcgttg agctgttcgg tgctcatttt
    32521 gtcgagccag ctagcagaga ggtttaaggc ggcaggttca tgaccagaag ggccatttaa
    32581 ggtttgtggc cagccgttat aaaacgcgct ggggcgagta tctatcgctt tgccattttg
    32641 ttgctgaagt tggtcaagtg taagaggctt cgccagttca gcggcccacg aagaagcgca
    32701 agccagccct aaagccattg ccagcgcggt catttgagaa acacgtttca tcgaaaatcc
    32761 cgacattata gttttgagtg cgccattctg aactgacgca ttgagaaaaa ctttgcgtta
    32821 tcgcagtttg ttgtaatttt ctgaccactg cgccatatcc agaacagaac tatcagcagg
    32881 aacatcctgg agatcgtgcg ttacctgaac gacggggatc gccagggcgc gaacttcgct
    32941 gaacacccac tggcgaaaat tatcgcgcag agccacatca agacggctga atggctcatc
    33001 caggagtaac gcttttggct gggcgagaag ggcgcgtagt agagcaacgc gcgctcgctg
    33061 accgccagac aaagtggcag gatcctgatg gaaagctccg tcaaggcctg aacgctcaag
    33121 tgcatcattc acggcattac gtcgggcatt ccccttaagt gtcgccggta gcgccagcag
    33181 taaattttgc ccgacgctga actggtcaaa taacagtgca tcctgaaaaa gaatgccaat
    33241 ctgacgctgt gcggtgggta gcatgtcaat ccgttgctca ttgagccata gctcacctgt
    33301 acaagaaaac tgtccggcca gtgcaccaat catccatgaa aacagagtgg attttccaca
    33361 gccagatggc cccattaacg tgacgatgtc acctttatca accgtaaagt taacgtttgt
    33421 cagcaagcgg ctttctggta aacgtagcga aacatttttc acgcagagca ttagcggatt
    33481 ccttgtctga cataacctac ccattttgcg actaacgcgg tcagggcaaa aataataagc
    33541 ggtaacaaca gttgccataa agcctgggcg gcgagaatac cgttgctgcc gccgctgctt
    33601 aatgccaccg cctcagtggt gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc
    33661 atatactgcg caatactgac tgaaaatccc accgcgaagg caatcagcac agggcgcaac
    33721 atgagtgggc atttcacgta aaagaagatt ttggcccgcg accagcccag tgtttgcgca
    33781 atcaaaatta accgtgaatc aatgcgctgc caggcgggtt gcaggataaa cagcatccac
    33841 ggcattaccc acagcagatg cccccagacc accgccgtcc agcttccatc caggttcagc
    33901 catagcgcca gcgtgtacta gcccgccacc agtggcagag cgggcaatag aatgggcaac
    33961 catagccaca actggcgacg ctgtgccccc cattccagcc acagcagtaa caggagcaaa
    34021 gcgatgaatg ttgctaccag ccccattgtc agactgttga tgagtgcttc actattgatc
    34081 gtcgactgat ccgcgaggat cgccagcaga accacacaca gcacaccggt taagggtaaa
    34141 aaactcgcca gcgtagtgcc tggtaataaa ggtgtggcag gcttgcgaat gccatctacg
    34201 cggggaatag tgcgccgcca gctgcgccat agcagatagc tcagcaaaac gtaggcggcg
    34261 agtaacagca tcagcagcag actggcgagt gcgcctttcg tttgttgatc cgcgtcgccc
    34321 tgagttaacc actgccagct aattactgcc agcgtcggtg gattacctgg cccaagaata
    34381 atcgccacat ccacgaccga aagcgaccag gcaacaatcg ccagcatcgc cattgccagc
    34441 gaaggcgcga cggagggcaa cagcagccag ttcaggcatt gccagcggct gtagcccagc
    34501 gaatccagca caatgacctg ctgcaacagc cgtttttcgc tcaatactgc cactaagatc
    34561 cacagcagaa atgcgctttc tttcactgca agggtgaggc ccagaccgat ccaaatcggt
    34621 ccattggcgg agtgaaatac gggaaatagt catatagcag ccctccgtca gcaaagagca
    34681 gcagggcgct ggtggcaaaa gccacatggg gaatggcgag cagccacggc agacgggcgc
    34741 acatacgctg ccatttcggc ccaggccata gcgccacaat caccagcagg gcaatcaaca
    34801 atgccccgac cgccgcgatg gttgtcgaca ccagcgttgc cagtaatgcc tgcggtaact
    34861 gtggatcggc aaataacgcc tgccagcgtg tcaacgacaa cgcaggcgat atcagcgtga
    34921 gagctgccgg gatcagcggt gcataaatca ccgccaccat cgcccacagc aaaaagatta
    34981 atgcgtaccg taacggcgta accattcttg ttccagtgca ttcacccatc ctgcgtgcgg
    35041 ttcagccagt accggcggca gatcctgcgg cattcttgat tgcaatattt cgcgctgccc
    35101 gtcaggcagt ttttgcggat cgagaacaga aggatcgccc cagacaacgg gatctgcttt
    35161 acgcagttgc gcatcgggtg agagcaggaa attggcaact accttcgccg cagcactggc
    35221 attcgcgttg gctggaatgg tgacgaaatg cacgttgcca atcatcccct cgcgaaagcc
    35281 aaaactgtaa ctgcttgccg gcaaatcgcc gctggcgatt ttttgctgcg catgcgcggg
    35341 gttaaaggtc agcgacaggc gcaatgtgcc ggctttcagc agagcatcca tccgcgcggg
    35401 cgaaggcggg aaatcctttc cttcgcgcca caaatacgga tgcagtgcat caagatattg
    35461 ccacaagggc gcagtgacac gggcgaaagt cgcatcgtcc ggcgcttctt ttaatgcggc
    35521 gggatctggc gtcagcataa tcagcaactg ttcaagaaac gccgtgccgg taaagtccgg
    35581 tgggcgcgga taagtaaccg tgccgggatt ggctttagca aactccagta aggcttgcgg
    35641 cgtttgtggt ggctgtggcg taacatcgcg gcgggcgata aacgtcagtt gtgcgccgcc
    35701 ccagggcgat tccgcacctt gtgtcggcac tgaaaaatct tcccgcaccg gcagctgtgt
    35761 gtcgacatag cgccagttgg gcagagtctc cgcccagccc gtttgtaata aattggcctc
    35821 tttta
//
LOCUS       DAJTYF010000027        35304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTYF010000027 DAJTYF010000000
VERSION     DAJTYF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.027"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..1165)
                     /gene="setB"
                     /locus_tag="OO590_001480"
     CDS             complement(<1..1165)
                     /gene="setB"
                     /locus_tag="OO590_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6636894.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EIHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVM"
     gene            1533..2663
                     /gene="fruB"
                     /locus_tag="OO590_001481"
     CDS             1533..2663
                     /gene="fruB"
                     /locus_tag="OO590_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6636895.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNCIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2663..3601
                     /gene="fruK"
                     /locus_tag="OO590_001482"
     CDS             2663..3601
                     /gene="fruK"
                     /locus_tag="OO590_001482"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6636896.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3618..5309
                     /gene="fruA"
                     /locus_tag="OO590_001483"
     CDS             3618..5309
                     /gene="fruA"
                     /locus_tag="OO590_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6636897.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5733..6674
                     /locus_tag="OO590_001484"
     CDS             5733..6674
                     /locus_tag="OO590_001484"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6636898.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYVDSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSASKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6662..7600
                     /gene="psuG"
                     /locus_tag="OO590_001485"
     CDS             6662..7600
                     /gene="psuG"
                     /locus_tag="OO590_001485"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6636899.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQIAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7694..8944
                     /locus_tag="OO590_001486"
     CDS             7694..8944
                     /locus_tag="OO590_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6636900.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9015..9713)
                     /gene="yeiL"
                     /locus_tag="OO590_001487"
     CDS             complement(9015..9713)
                     /gene="yeiL"
                     /locus_tag="OO590_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCR6636901.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            9844..10785
                     /gene="rihB"
                     /locus_tag="OO590_001488"
     CDS             9844..10785
                     /gene="rihB"
                     /locus_tag="OO590_001488"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6636902.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMAGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10885..12135
                     /locus_tag="OO590_001489"
     CDS             10885..12135
                     /locus_tag="OO590_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6636903.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLVLNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12191..13279)
                     /locus_tag="OO590_001490"
     CDS             complement(12191..13279)
                     /locus_tag="OO590_001490"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6636904.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLVDCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13282..14138)
                     /gene="nfo"
                     /locus_tag="OO590_001491"
                     /pseudo
     CDS             complement(13282..14138)
                     /gene="nfo"
                     /locus_tag="OO590_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
     gene            complement(14212..15261)
                     /locus_tag="OO590_001492"
     CDS             complement(14212..15261)
                     /locus_tag="OO590_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6636905.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15360..16241
                     /locus_tag="OO590_001493"
     CDS             15360..16241
                     /locus_tag="OO590_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6636906.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16447..17916
                     /gene="lysP"
                     /locus_tag="OO590_001494"
     CDS             16447..17916
                     /gene="lysP"
                     /locus_tag="OO590_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6636907.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18208..20199
                     /gene="cirA"
                     /locus_tag="OO590_001495"
                     /pseudo
     CDS             18208..20199
                     /gene="cirA"
                     /locus_tag="OO590_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20231..21067)
                     /gene="yeiG"
                     /locus_tag="OO590_001496"
     CDS             complement(20231..21067)
                     /gene="yeiG"
                     /locus_tag="OO590_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6636908.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21325..21993
                     /gene="folE"
                     /locus_tag="OO590_001497"
     CDS             21325..21993
                     /gene="folE"
                     /locus_tag="OO590_001497"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6636909.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22010..23167
                     /locus_tag="OO590_001498"
     CDS             22010..23167
                     /locus_tag="OO590_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6636910.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23309..24348
                     /gene="galS"
                     /locus_tag="OO590_001499"
                     /pseudo
     CDS             23309..24348
                     /gene="galS"
                     /locus_tag="OO590_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            24628..25626
                     /gene="mglB"
                     /locus_tag="OO590_001500"
     CDS             24628..25626
                     /gene="mglB"
                     /locus_tag="OO590_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6636911.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25687..27207
                     /gene="mglA"
                     /locus_tag="OO590_001501"
     CDS             25687..27207
                     /gene="mglA"
                     /locus_tag="OO590_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6636912.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27223..28233
                     /gene="mglC"
                     /locus_tag="OO590_001502"
     CDS             27223..28233
                     /gene="mglC"
                     /locus_tag="OO590_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6636913.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28304..29539)
                     /gene="preA"
                     /locus_tag="OO590_001503"
     CDS             complement(28304..29539)
                     /gene="preA"
                     /locus_tag="OO590_001503"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR6636914.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29533..30773)
                     /gene="preT"
                     /locus_tag="OO590_001504"
                     /pseudo
     CDS             complement(29533..30773)
                     /gene="preT"
                     /locus_tag="OO590_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(30967..31206)
                     /locus_tag="OO590_001505"
     CDS             complement(30967..31206)
                     /locus_tag="OO590_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6636915.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31209..31928)
                     /gene="sanA"
                     /locus_tag="OO590_001506"
     CDS             complement(31209..31928)
                     /gene="sanA"
                     /locus_tag="OO590_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6636916.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32078..32962)
                     /gene="cdd"
                     /locus_tag="OO590_001507"
     CDS             complement(32078..32962)
                     /gene="cdd"
                     /locus_tag="OO590_001507"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6636917.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33092..33787)
                     /locus_tag="OO590_001508"
     CDS             complement(33092..33787)
                     /locus_tag="OO590_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6636918.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33784..34182)
                     /locus_tag="OO590_001509"
     CDS             complement(33784..34182)
                     /locus_tag="OO590_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6636919.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34421..>35304
                     /gene="dusC"
                     /locus_tag="OO590_001510"
     CDS             34421..>35304
                     /gene="dusC"
                     /locus_tag="OO590_001510"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6636920.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8670 a         8630 c         9321 g         8683 t
ORIGIN      
        1 tcattaccca aaacagagtg gcgataatca tcaccatcgc aaaccagaac acagcgtgat
       61 aattccagat ctcggcgacg atgcccgcca ctgatcctgc gatgatccag cccacgcgcg
      121 acgtgttggt atagagcgtg gtggctgaac ctgcctgacc gggcatcaaa tcctgaaaat
      181 agagcatccc gatgccgccc agaatgccaa taaaaatagc atttagcagc tgcaagccca
      241 acagaatggc aggtgaatgc gccatcagca ttcctgcgta aaaacagacg ccacccacgg
      301 cagcaacgcg cattaagaaa cgcttaccca gacgtttggc gaaatatccg gcaatcaaca
      361 tcgtcgggat ttccagcccg gcggcggtcc ccatcatcac accggccagt ttctcgggaa
      421 gatgcagttc gttgataata aatagcggca tgttgatgat gtacaggctg ttcgagcccc
      481 acatcaatgt acaaatgaca aacagcagta gcgtatcgcg acggttacgg cgcggcgctt
      541 caaccgtgcc ggtcgccagc ggaagctctt tttgcatcga cggtaaaaac agccacacca
      601 tcacgccgca aacaataaac gctaccgctg cgctcagata cattaccgta aagctgaaac
      661 ccatcgctaa cgcataagcc agcggtgggc caatgaccca tgccagtgaa acctgagcgc
      721 gtaaaaaaga gctgaacatc accgcctcac gtccggtttt gtcggcatgt tcacgggcaa
      781 gggcaaacat ttgcgggtta gcggtcgagc caaagctgct aagaaagacg ccaacgaata
      841 gcaaaacaaa gtagttgcga ttccaggcaa aaagggtgca ggccagcacg cctaacaggc
      901 agcaaaagac aatcagcgat ttgcgatcgc cgcgcttatc agagcgccca gcgagaaact
      961 gacttaccag aatcccaatg acagcgctgc cggtaaagaa gaatcccacc atcgccggac
     1021 gggcatgtat ttcatcggta aggaaaatac tgagtgtcgg ggtttgcaga gcacccgcaa
     1081 taccggtgag aaaggcaacg attaaaaacg ccgtcgaggt caggtcaaac gatttcgcgc
     1141 tggagactgc gggggagtta tgcatgctgg tcgatcactg gaagaggaag acgcggagtt
     1201 tacgctgctt gtcagaaaat aaacagacag cgaaatcaaa agcgccacga ataatcaaaa
     1261 tgccatttca ctgttgctga ttggctgctg acgctgctga aatgaaattg ctgataacgg
     1321 attttcccat cagcaattag gaaaaatgac aaaaaattgt gcagcacatc aaacttttgc
     1381 tcataacttt acggctttcc ttgcgtgctg aaacagaagt attatgcttt cttgaaacgt
     1441 ttcagcgcga tcttgtcttt aaccctaagc caggttggcg ctttttttct catagaggct
     1501 gaatcgtttc aattcagcaa gagaggagaa ctatgttcca gttatccgta caggacatcc
     1561 atccgggcga aaaggccgga gacaaagaag aggcgattcg ccaggtcgct gcggcgctgg
     1621 tgcaggccgg taatgtagca gaaggctacg tcaatggcat gctggcgcgc gaacagcaaa
     1681 cctcaacgtt cctcggcaat tgtattgcta ttccacacgg cactaccgac acccgcgatc
     1741 aggtgctgaa aaccggcgtt caggtatttc agttcccgga aggcgtcacc tggggtgacg
     1801 gtcaggtagc gtacgtggca atcggtattg ctgccagctc ggataaacat ctgggcctgc
     1861 tacgccagct gacccacgta ctgagcgatg attccgttgc tgaacaactg aagtcagcaa
     1921 caacagcaga agaacttcgc gcattactga tgggcgaaaa gcagagtgag cagctgaagc
     1981 tcgacaacga aatgctgacg ctggatatcg tcgccagcga tctgctgact cttcaggcgc
     2041 tgaacgctgc gcgtctgaaa gaggcgaggg cagttgacgc cactttcgtc accaaagcca
     2101 tcaatgaaca accgctgaat ctcggacagg gtatctggct gagcgatagc gccgaaggca
     2161 atctgcgtag tgcgattgcg gtaagccgtg cggcaaatgc ttttgatgtg gacggcgaaa
     2221 cggcagccat gctggtgagt gtggcgatga atgacgatca gcccatcgcg gttcttaagc
     2281 gtctcgctga tttgttgctc gacaataaag ctgaccgctt gctgaaagcg gatgcggcaa
     2341 cgttgctggc gctgctgacc agcgatgatg cgccgaccga cgacgtgtta agcgcggagt
     2401 ttgtggtgcg caatgaacac ggcctgcatg ctcgtccagg taccatgctg gtcaatacca
     2461 ttaaacaatt taacagtgat attaccgtga caaaccttga tggcaccggc aaaccggcaa
     2521 acggacgtag tctgatgaaa gttgtggcac ttggcgttaa gaaaggtcat cgcctacgct
     2581 ttaccgccca gggtgcagat gctgaacagg cgctgaaagc cattggcgac gctatcgctg
     2641 ctggtcttgg ggagggcgca taatgagcag acgtgttgct acgatcaccc ttaatccggc
     2701 ttatgacctt gttggtttct gcccggaaat tgaacgcggc gaagtgaacc tggtgaaaac
     2761 caccggtctg catgcggcgg gtaaaggcat caacgtggcc aaagtattaa aagacctggg
     2821 aattgatgtc accgttggcg gcttcctggg taaagacaat caggatggtt ttcagcaact
     2881 gttcagcgag ctgggcattg ccaaccgttt ccaggttgta caggggcgca cccgaattaa
     2941 cgttaagctg acggaaaaag acggcgaagt gaccgacttc aacttctcgg gttttgaagt
     3001 cacccccgcc gactgggaac gctttgtgac tgattctctg agctggctcg gtcagttcga
     3061 tatggtctgt gtcagcggaa gcttaccgtc aggcgtcagc ccggaagcgt tcaccgactg
     3121 gatgactcgc ctgcgtagtc agtgtccttg cattatcttt gatagtagcc gtgaagcgtt
     3181 agtagcaggt ttgaaagcgg caccgtggct ggtgaaacct aaccgccgcg agttggaaat
     3241 ctgggcaggc cgtaaactgc ctgaaatgaa agatgtgatt gaagctgcac atgcgctacg
     3301 tgaacaaggt attgcgcatg ttgttatttc actgggtgcc gaaggcgcgc tttgggttaa
     3361 tgcctccggc gaatggatcg ctaaaccacc gtcagtcgat gtcgtaagca ccgttggcgc
     3421 aggggattct atggttggtg gcctgattta tggcttgctg atgcgtgaat ccagtgaaca
     3481 cacactgcgt ctggcgacag ctgttgcagc cctggcggta agtcaaagca atgtgggtat
     3541 taccgatcgt ccgcagttgg ccgcaatgat ggcgcgcgtc gacttacaac cttttaactg
     3601 acagcaggag aggcataatg aaaacgctgc tgattattga cgctaatctc ggtcaggcac
     3661 gcgcctatat ggcgaagacc ctgctgggcg cggcggcgcg aaaagcaaaa ctggaaatca
     3721 tcgacaatcc gaacgacgca gaaatggcga ttgttctcgg tgattccatc ccgaatgaca
     3781 gcgcgctgaa cggtaaaaat gtctggctgg gtgatatttc ccgggcagtt gcgcaccctg
     3841 agctgttcct gagtgaagcc aaaggccatg cgaaacctta cactgcgccg gtcactgcga
     3901 cagcaccggt tgccgccagc ggtccgaaac gcgtagttgc ggtgactgct tgcccgactg
     3961 gcgtagcaca cacctttatg gcggctgaag ccattgaaac cgaagcgaaa aaacgtggct
     4021 ggtgggtgaa agttgaaacc cgtggttctg ttggcgcggg taatgcaatc actcccgagg
     4081 aagtagccgc agcggatctg gtgattgtgg cggcagatat cgaagtggat ctggcgaaat
     4141 ttgctggtaa accgatgtat cgtacctcta ccggtctggc gctgaagaaa acctcgcagg
     4201 aactggataa agcggttgct gaagcaacgc cgtatgaacc ggcgggcaaa gctcaaacgg
     4261 cgaccactga aggtaagaaa gagagtgcag gcgcttatcg tcacttgcta acgggcgtct
     4321 cttacatgct gccgatggtc gttgctggtg gtctgtgtat cgcgctttct tttgcttttg
     4381 gtatcgaagc gtttaaagag ccgggtacgt tggcagcggc gctgatgcag attggtggtg
     4441 gttcagcctt tgcgctgatg gtgccggtac tggcaggtta tattgccttc tccattgccg
     4501 atcgtccggg cctcacgccg ggtctgattg gcggtatgct ggcggtcagc accggttctg
     4561 gcttcattgg cggtattatt gcgggcttcc tggctggtta cattgcgaag ttaatcagta
     4621 cgcaactgaa actgccgcag agtatggagg cgctgaaacc gatcctgatc attccgctaa
     4681 tttccagtct ggtggtcggt ctggcgatga tctacctgat cggtaaacca gttgctggca
     4741 ttctcgaagg gctgactcac tggctgcaga ccatggggac tgcgaatgcg gttctgctgg
     4801 gggcgatcct cggtggcatg atgtgtactg acatgggcgg tccggtaaac aaagcagcgt
     4861 acgcattcgg tgtgggtctg ctgagtactc aaacctatgg cccgatggcg gcgattatgg
     4921 cggcaggtat ggtgccaccg ctggcaatgg gcctggcaac aatggtggcg cgtcgcaaat
     4981 tcgacaaagc gcagcaggaa ggtggcaaag ccgctctggt attggggctg tgcttcattt
     5041 cggaaggtgc aattccgttt gctgctcgtg atccgatgcg tgtgctgccg tgctgtatcg
     5101 tgggtggggc gctgactggc gcaatctcaa tggcgattgg tgcgaaactg atggcaccgc
     5161 acggtggtct gtttgttctg ctgatccctg gcgctattac gccggtattg ggttacctgg
     5221 tagcaattat tgccggtacg ctggtggcgg gtttggccta tgccttcctg aaacgtccgg
     5281 aagtggacgc agtagcgaaa gcagcgtaat aaaaggtgtg ttacagggca gaaatttcct
     5341 gccctgtaac atttgatgag cgcaaagaga atgaccagct tgtgaatgta attttatgaa
     5401 tttaaatggt tttatctttc agtagaaact tcaaacgttc atcaaacact aaggttattt
     5461 cctcaagggc agattatttt ctgccctttt ttagtgttcc tgttctgtgt atcccgtcac
     5521 aaattcctcg cttttgtaat tcaggcattt aatcgcattt tgtgagcctg ctcaaatctg
     5581 cccattttgc ctctctggcg gtaatttact ttacgtaaaa catgcatact atgagcacat
     5641 gtttaaatat gaacacatgt ttaaaccgtc tttgcagtat ctatattctt gtggattgac
     5701 agggttggta attttttacc agaggacgtg ctatgcgcga aaaggattat gtcgtaatta
     5761 taggttcggc gaatattgat gtcgccggat attcacatga atcattaaat tatgtggatt
     5821 caaatccagg taaaataaaa tttacgcctg gtggagtagg gcgcaatatt gcacaaaacc
     5881 tggcgttgct gggtaacaaa gcctggctac tgagcgccgt aggcagtgat ttttatggtc
     5941 aatcgctgct aacgcaaacc aatcaatctg gtgtttatgt cgataaatgc ctgattgtgc
     6001 cgggagaaaa tacgtcgagt tatttatcat tactcgataa taccggtgaa atgctggttg
     6061 ctataaatga catgaatatt agcaacgcta ttacagctga atatctcgca cagcaccgtg
     6121 aatttattca gagggcaaag gtcattgtcg cggactgtaa tatcagtgaa gaggcactgg
     6181 catggattct ggataatgcc gccaacgtac ccgtatttgt cgatccggtt tcagcgtcga
     6241 aatgtgtcaa agtgcgcgac cgtctaaatc agatccacac tctgaagcca aaccgccttg
     6301 aagcggaaac cctgagtggg attgcgctgt cagggcgtga cgatgtggca aaagttgctg
     6361 cctggttcca tcaacatggc ctgaaccgac tggtattgag catgggcggc gacggcgttt
     6421 attacagcga tatcagcggt gaaaatggct ggtctgcgcc gatcaaaacc aatgttatta
     6481 atgttaccgg agcgggcgat gccatgatgg cgggacttgc ttcgtgttgg gtagacggaa
     6541 tgccgtttgc cgaatctgtt cgtttcgcac agggatgttc gtcaatggcg ctctcctgtg
     6601 aatacaccaa taaccccgat ttatcgattg ccaacgttat atcgttagtg gagaacgcag
     6661 aatgtctgaa ttaaaaattt cccctgaatt attacaaatt tctccggaag tgcaggacgc
     6721 tttaaaaaac aaaaaaccgg ttgtggcgct ggaatcaacc attatttctc acgggatgcc
     6781 gttcccacaa aatgcccaga tcgcaattga agtagaagaa actattcgta aacagggcgc
     6841 agtacctgcc actatcgcca ttattggcgg cgtgatgaaa gtgggtttaa gcaaagaaaa
     6901 aattgaatta ctgggtcgtg aagggcataa cgtgaccaaa gttagtcgtc gcgatttacc
     6961 ttttgttgtt gccgccggaa aaaatggcgc aaccactgtg gcttcaacga tgattattgc
     7021 ggcgcttgcc ggaattaaag tatttgccac cgggggaatt ggtggtgtgc atcgcggggc
     7081 ggaacatacc ttcgatattt ctgccgattt gcaagaactg gcaaatacta atgtcaccgt
     7141 tgtttgtgcc ggggcgaaat ctattctcga tttaggatta accactgagt atttagaaac
     7201 cttcggtgtg ccgttaattg gctatcagac taaagcgctg cctgcgtttt tctgccgtac
     7261 cagctcgttt gacgtcagca ttcgtctcga cagcgccagt gaaattgccc gtgcaatggc
     7321 ggtgaaatgg caaagcgggc tgaacggtgg cctcgtggta gcgaacccga tcccggaaca
     7381 gtttgcgatg ccggaggaat ctatcaatgc agccatagat caagccgtcg ccgaagccga
     7441 agagcagggc gttattggta aagaaagtac accgttcctg ctggcgcgcg ttgctgagct
     7501 gaccggcggt gacagcctga aatccaatat ccagctggtg ttcaacaacg ccattctggc
     7561 gagtgaaatt gccaaagaat accagcgtct cgcgggttaa tttctgacgg gcagaaagcc
     7621 gtcgcccgta ctcatcaagc ataccaaaac ctggcgctga cgccgggttt actggcatga
     7681 agggaaaatc attatggata taatgagaag tgttgtgggg atggtggtgt tactggcaat
     7741 agcatttctg ttgtcagtga ataaaaagag catcagtttg cgcacggttg gagccgcact
     7801 gctgctgcaa atcgctattg gtggcatcat gctctacttc ccaccgggaa aatgggcagt
     7861 agaacaggcg gcattaggcg ttcataaagt gatgtcttac agtgatgccg gtagcgcctt
     7921 catttttggt tcgctggttg ggccgaaaat ggatgtcctg tttgacggtg cgggttttat
     7981 cttcgccttt cgcgtacttc cggcgattat tttcgttact gcgctcatca gtctgctgta
     8041 ctacattggc gtgatggggc tgctgattcg catccttggc agcattttcc agaaagccct
     8101 caacatcagc aaaatcgaat cttttgttgc ggttaccact attttcctcg ggcaaaatga
     8161 gatcccggcg atcgttaaac cgtttatcga tcgcgtgaat cgcaacgagt tgtttaccgc
     8221 aatttgtagc gggatggcgt ccattgctgg ttcgatgatg attggttatg ccggaatggg
     8281 cgtaccaatt gactacctgt tagcggcatc gctgatggcg atccccggcg gtattttgtt
     8341 tgcacgtatt cttagcccgg caaccgagcc ttcgcaggtc acatttgaaa atctgtcgtt
     8401 cagcgaaacg ccgccaaaaa gctttatcga agcggcggcg agcggtgcga tgaccgggct
     8461 gaaaatcgcc gctggtgtag cgacggtggt aatggcgttt gtcgcaatta ttgcgctgat
     8521 caacggcatt atcggcggaa ttggcggctg gtttggtttc gccaatgcct ctctggaaag
     8581 tatttttggc tatgtgctgg caccgctggc gtggatcatg ggtgtggact ggagtgatgc
     8641 caaccttgct ggtagcctga ttgggcagaa actggcgatt aacgaattca tcgcttacct
     8701 gagtttctcc ccatacctgc aaacgggcgg cacgctggaa gtgaaaaccg tggcgattat
     8761 atcctttgcg ctttgtggtt ttgctaactt tggttctatc ggtgttgtcg ttggcgcatt
     8821 ttcggctatt tcgccaaaac gtgctccaga aatcgcccag cttggtttac gggcgctggc
     8881 agcagcaacg ctttccaacc tgatgagtgc gactattgcc gggttcttta ttggtctggc
     8941 gtaatgctcg atttgtagac cggataaaac gtattaagcg ttgcatccgg caatcgcgct
     9001 attgcaaata atttttactg catcatcccg gagaatttat tctccgggtc catctccagc
     9061 gccagtcctg acaactgctt tctgttttta attagatatc ctttcttgct tttaattaat
     9121 aaaccgtcgt gaatgaattg cgcgagaaca tataaaagat gtcgataaga aacgcctaaa
     9181 tactctgccg cttgcgtatg cttttcgtga taaagatcac cttcttgtga gagtaatata
     9241 aatgctgcca ggcgattcat taacggaaat gattgattct gagttaaaga aacaatatta
     9301 cgataatttt tatgacttaa ggtgacgcaa agttttcgta aaaatagggt gtcgtttaat
     9361 aacagcggac ggtaatgttt catagggagc gcaaggcacc aacactcttc aatagcctgc
     9421 accgcacgcg gttcatggtc tttgtcgatt aactcaatct cgccaataaa acagggagca
     9481 gcaaagaaat cgatcagcga cacgcgacca ttagcaagcg tggcgtaaag cctggcgcgg
     9541 cctcgcgtca gataaaacag ccatgacggc tgttgccctt cctgcacaat gtagtcacgc
     9601 gctaaaaaat gaaacagccg cgtatcggct gaaacatccg ttgaaaagca atccttaaac
     9661 gcagattcac tcatcaattg ctgcttaagg tcattattgt ggatttcttt cacgatgttt
     9721 atccgctatg agatttctca tatttagcat acgtacgctg ttgttaagat tcaatcatca
     9781 gcccggtcga attttctttg ctgtagaaaa aaataacgca actggaaaca gaggaaataa
     9841 acaatggaaa agagaaaaat tattctggat tgtgagccgg gtcatgatga tgctattgca
     9901 atgatgatgg cagcgaaaca tccggcaata gatttattag gcatcactat tgtagcgggt
     9961 aatcagacgc ttgataaaac attaattaat ggcctgaatg tttgccagaa actggagatt
    10021 aatgttccgg tttatgcggg gatgccgcaa cccattatgc gtaaacaaat cgttgccgat
    10081 aatattcacg gtgaaaccgg actggatggc ccggtattcg agccgctgac ccgccaggca
    10141 gaaagtaccc atgcggtgaa atatatcatc gataccctga tggcaagcga tggcgatatc
    10201 actctggtgc cggttggtcc gctttcaaat atcgcggtgg caatgcgtat gcaacccgca
    10261 atcctgccca aaatccgtga aattgtgcta atggccggcg cttacggtac aggcaacttc
    10321 accccatctg ccgagttcaa tatctttgcc gacccggaag ccgcccgcgt ggtgttcacc
    10381 tccggcgttc cattagtgat gatgggcctc gatcttacca accagaccgt ttgcaccccg
    10441 gatgtaattg ctcggatgga aagggtaggc ggccctgccg gagagctgtt cagcgacatc
    10501 atgaacttca ctctcaaaac gcagttcgaa tactacggcc tggctggcgg cccggtgcat
    10561 gacgccacct gcattggtta tctgatcaac cctgatggca ttaaaaccca ggatatgtac
    10621 gtcgaagtgg acgtcaacag cggcccttgc tacgggcgta cggtctgcga cgaactgggc
    10681 gttcttggca agcccgctaa taccaaagtc ggcatcacta ttgataccga ctggttctgg
    10741 ggcttagtcg aagagtgcgt gcgcggctac atcaaaaccc attaagtgtt gataacaagc
    10801 cgggacagta tcgcaagatg caggcccggc tatttctgat gggcagaaag ccgtcgccca
    10861 aaatgacatg aagagataat cactatggat gtcatgagaa gtgttctggg aatggtggta
    10921 ctgctggcaa ttgcgttttt actgtcagta aacaagaaga agatcagcct gcgtaccgtt
    10981 ggcgcggcgt tagtgttaca ggtcgtgatt ggcgggatta tgctttggtt accgccaggg
    11041 cgttgggtcg cagaaaaagt cgcttttggc gtccacaaag tgatggcgta cagcgacgca
    11101 ggtagcgcat ttatcttcgg ttctctggtc ggaccgaaaa tggatacctt atttgatggc
    11161 gcaggattta tctttggttt cagggtatta ccggcaatta tcttcgtcac tgcactggtg
    11221 agtattctct actacatcgg tgtgatgggg atcttaattc gcattctcgg cggtatcttc
    11281 cagaaagctt taaatatcag caagatcgag tcattcgtcg cggtcaccac cattttcctc
    11341 gtgctaaacg aaattccggc gatcgtcaaa ccctttatcg atcgtctgaa tcgcaatgaa
    11401 ttatttacag cgatttgtag tggcatggcc tcgattgctg gttcgacaat gattggttac
    11461 gccgcactgg gcgtacctgt ggaatatttg ctggcggcat cgttaatggc gatccctggc
    11521 gggatcttgt ttgcccgcct gttaagcccg gctacggaat cttcgcaggt ttcttttaat
    11581 aacctctctt tcaccgaaac accgccaaaa agcattattg aagccgatgc gacaggggca
    11641 atgaccgggc tgaaaatcgc cgccggtgta gcgacagttg ttatggcatt tgtcgccatc
    11701 attgcgttaa ttaacggtat tatcggcggc gttggcggct ggtttggttt tgaacatgcc
    11761 tcgctggagt ccattttagg ttacctgctg gccccacttg cgtgggtaat gggtgttgac
    11821 tggagtgatg caaatcttgc cgggagtttg attggacaga aactggcaat aaatgaattt
    11881 gtcgcttatc tcaatttctc accctatctg caaacggctg gcactctgga tgctaaaacc
    11941 gtggcgatta tttccttcgc gttgtgcggt ttcgctaact ttggttctat cggggtggtg
    12001 gtgggggcgt tttctgcggt tgcgccacac cgtgcgccgg aaatcgccca gcttggttta
    12061 cgcgcgctgg cggcggcgac actttctaat ctgatgagtg ccaccattgc cgggttcttt
    12121 attggtttag cgtagtgtgc aatttatgag ccggattaga tgcggtcaag cgtcgaatcc
    12181 ggcaacattg ttacaccatt ggcactaatg acagcgcgtt atcggcagac agggtagggt
    12241 tgtttaagct gccgctggcg cgtgaaattg ccgcgcaggc catcgcaaaa cgggcgctgt
    12301 cacggaaatt gtttccctcc agaaagctat ataccagacc cgccataaaa ccatcgtcag
    12361 caccaaaact gtctaccgtc gtatgcgctg gcacagtcag caaaaattgt tcaccatcct
    12421 tttcgctgca ataaactgac tcatcgggca aataaacaaa cagttgctga acaccttgct
    12481 gatgcaatgc attcactgcg gcattacggt cagcatcgct ggtgatcgcc tgtccccata
    12541 aaatttccag ctccggtaaa gtgggtttca gggtgtgaat atgcgccagc cagtgtttga
    12601 ttttgcccgc tttgaattct gaaacggtat cgacaaacac cgggatctca tcagcgaggg
    12661 taaagaccca ttccagcgcc tcggctgtca ggttgcaatc aaccagtacc acgcccgcat
    12721 gacgaagtaa atcgcgcgac ccgttcagta gttgcggtgt caactgttcc agcagatggg
    12781 tatcgttaat cgccagcacg gtttgatcgt ctcgattggc aattgccaga tacgtcgatg
    12841 tgctttgacc atgcaaacga acgcagccgg agacattcac gccggcacgg cgcgtttctt
    12901 ccaggagcat ttcgccataa aagtcatcgc caatcactga aagcaaatgg acgtcacggc
    12961 ctaacagcgc cagattgtgg gcgatattgc gtccaacgcc gcctgccgaa caatgaattg
    13021 taccgggatg agaagccgct tgcgggtaat ggatatccgc catcccgcga atatccatat
    13081 tgattgtccc caccactacg cagtattcct gctcggtgag aatgtaacct ttgcctttaa
    13141 tccggccttt gcgcattaaa tccataatat gcgccgcaac gcgcgaacga ctgatttgca
    13201 gcatgtccgc aatttcgttc tgctgaatca gcgggttacg ccgtaaaatt gcaaggatct
    13261 ccttttcccg gttattcatc ttcaggctac cgctttttca gtttgttgcg ctttcagcca
    13321 ggcgatctct tctgcccaga tatccgggtt gatggtttca aggatcagcg gaatgccgtc
    13381 gaaacggtcg ttctgcataa tccagcggaa cgcatcatga ccgatattgc cttcaccgag
    13441 gctatgatgg cggtcaacgc ggctgccaaa ggtacttttc gcatcgttaa ggtgcatccc
    13501 gcgcagatac ttaaagccga caatacgggc aaaatcagcg aacgtttttt cgcattcggc
    13561 tggagtacgc aaatcatacc cggcagcgaa agcatggcag gtatcaatgc agacgccgac
    13621 gcgggattta tcttccacgc cgtcgataat cgccgcgaga tgttcgaatt taaaccctaa
    13681 gttactgccc tgaccggcgg tgttttcaat caccgctgtt acaccttgag ttttatccag
    13741 cgcaatgttg atggattcgg caatacgcgc aaggcaatcc tcttctgaaa tctgcatcag
    13801 atggctgcca gggtggaagt tgagcaaaga aagccccagt gttcgcaacg ctgcatttca
    13861 tctataaagg catcgcgcga tttttccaga gcttcagtga ccggatgtcc gaggttgatc
    13921 agataactgt cgtggggaag aatttgcgcc gatgtgtagt gatatttttc acaggcggct
    13981 ttgaattcat cgatggtttg cgtcgtgagc ggtgcggcac gccactgacg ttggtttttg
    14041 gtgaacaagg caaacgcggt tgcgtcgatt tcggcggcgc gaattgcggc atttgccaga
    14101 ccgccagcag cactaacgtg cgctccaatg tatttcatgc gaggactcct gttaaacccg
    14161 ctggaggaaa acggtaatga tagcgggtta acaggagcaa gaagtagtgg tttatgcgat
    14221 gacgctttga atcacatagt taatcgcacc accaccaaca atcagccagg caaacagtac
    14281 cagtgccatc agcagaggtt tcgccccagc ttttttcagc gcgctgacgt gagtggtcag
    14341 acccagcgcc gccatcgcca ttgccagcag gaaggtatcc agcgttacca gcatgttcac
    14401 cacgctctgc ggtaacaggt ggaacgagtt aaagatggca actacgatga acaagatggc
    14461 aaaccacgga atagtgattt tgcttttctc gccgctgttc gccccagaca gctgtttaac
    14521 acgcgccgcc agcaggatga ggaacggagc cagcatcatc acgcgcagca ttttggaaat
    14581 aactgctgcg ttttctgcat ccgggctgat ggcatgacct gccgccacca cctgcgccac
    14641 ttcgtgcaca gtagaaccaa tgtagatacc gaaagtttcc ggactaaacc attgagacat
    14701 cagcggatat atcgccgggt agaggaaaat cgcgacggtc ccgaagataa caacggttgc
    14761 aacagccacg gttactttac tggcttccgc tttcactacc ggctcagtcg ccagtaccgc
    14821 ggcagcacca cagatactgc taccggcacc gatcaaccaa ctggtgtgct tatccagacc
    14881 aaacactttc tgccccagga agcaagccag caggaaggta ctggacagcg tcagcacgtc
    14941 aatgatgatc ccactgatac cgacatcggc aatttgcgag aacgtcagac ggaagccata
    15001 aagaatgata cccagacgta ataaatattg cttggcaaac agcacaccac cgtcacagct
    15061 tttccagatg tgcggataga tggtgttgcc taaaaccatc cccaacaaga ttgcgagggt
    15121 gagggcacta aacccggcac ccgcaaccgc gggaatggaa ccaccccaca gggcgacccc
    15181 ggtgataact gcactcaggg ctaaccccgg aataaaatgc cacagtgtac gatgttgttt
    15241 ctgtaaggtg atattcgtca taaccctctc ctttaaccgg gcataaggtt acgactaact
    15301 ggtttaaaaa taaaattgat tatatattta taattaatct ttataagtgg taagcgacta
    15361 tgcacatcac cctccggcag ttggaagttt ttgcagaagt attgaaaagt ggatcaacca
    15421 cccaggcgtc ggtgatgctg gcgttgtcgc aatcagcagt gagcgcagcc ttgactgacc
    15481 tggaagggca gcttggcgtt caactgtttg atcgcgtggg gaaaagactg gttgttaatg
    15541 aacacgggcg gctgctctat ccgcgagcgt tggcactgct tgaacaggcg gttgaaatcg
    15601 aacaactgtt tcgcgaagac aacggcgcga ttcgtatcta tgccagtagt accatcggta
    15661 actacattct gcctgcagtt atcgcccgtt atcgccatga ttatccgcag ttgccgattg
    15721 aacttagcgt tgggaacagc caggacgtga tgcaagcggt gctggatttc cgcgttgata
    15781 ttggctttat tgaaggaccg tgccacagca ctgaaatcat ttctgaaccg tggctggaag
    15841 acgagctggt ggttttcgcc gcgccgactt cgccgttggc tcgtggtccg gtcaccttag
    15901 aacagctggc cgctgcgccg tggatcctgc gtgaacgcgg ttccggcacg cgggagattg
    15961 tcgattatct gttgctgtca catttaccga agtttgagat ggcgatggaa ttaggtaact
    16021 ccgaggcaat caaacatgcg gtgcgtcatg ggttgggaat tagttgcctg tcgcgacgtg
    16081 tgattgaaga tcaattgcag gcaggcacat taagtgaagt tgcggtccct ctgccgcgcc
    16141 tgatgcgtac gttgtggcgt atacatcatc ggcaaaaaca cctttccaac gcgctacggc
    16201 gctttctgga ctattgcgat cccgcaaatg tgccgcgtta agttgctgta caagaacatg
    16261 ctggtgctgt gtcgatttcg tgacgcagcg ccttcagcat gcattcgcca gaaaagagat
    16321 tggctgcttt acttataatc cctgggcgat catgaaggtg tcttataacc gtgtatttct
    16381 gccggaagga ttgccaatcg tctgctacaa tcgcgccatc atttttaaga tggatagcat
    16441 ttttgtatgg gttccgaaac taaaactaca gaagcgccgg gcttacgccg tgaattaaag
    16501 gcgcgtcacc tgacgatgat tgccattggc ggttccatcg gtacaggtct ttttgttgcc
    16561 tctggcgcaa cgatttctca ggcaggtccg ggcggggcat tgctctcgta tatgctgatt
    16621 ggcctgatgg tttacttcct gatgaccagt ctcggtgaat tggctgcata tatgccggtt
    16681 tccggttcgt ttgccactta cggtcagaac tatgttgaag aaggctttgg cttcgcgctg
    16741 ggctggaact actggtacaa ctgggcggtg actattgccg ttgacctggt tgcagcacag
    16801 ctggtcatga gctggtggtt cccggataca ccgggctgga tctggagtgc gttgttcctc
    16861 ggcgttatct tcctgttgaa ctacatctct gttcgcggct ttggtgaagc ggaatactgg
    16921 ttctcactga tcaaagtcac gacagttatt gtctttatca tcgttggcgt gctgatgatt
    16981 atcggtatct tcaaaggcgc gcagcctgcg ggctggagca actggacaat cggcgaagcg
    17041 ccgtttgctg gtggttttgc ggcgatgatc ggcgtagcta tgattgtcgg cttctctttc
    17101 cagggaaccg agctgatcgg tattgctgca ggcgagtccg aagatccggc gaaaaacatt
    17161 ccacgcgcgg tacgtcaggt attctggcga atcctgttgt tctatgtgtt agcgatcctg
    17221 attatcagcc tgattattcc gtataccgat ccgagcctgc tgcgtaacga tgttaaagac
    17281 atcagcgtca gtccgttcac cctggtgttc cagcacgcgg gtctgctctc tgcggcggcg
    17341 gtgatgaacg cagttattct gacggcggtg ctgtcagcag gtaactccgg tatgtatgcg
    17401 tctactcgta tgctgtacac cctggcgtgt gacggtaaag cgccgcgcat tttcgctaaa
    17461 ttgtcgcgtg gtggcgtgcc gcgtaatgcg ctgtatgcga cgacggtgat tgccggtttg
    17521 tgcttcctga cctccatgtt tggcaaccag acggtatacc tgtggctgct gaacacctcc
    17581 gggatgacgg gttttatcgc ctggctgggg attgccatta gccactatcg cttccgtcgc
    17641 ggttacgtat tgcagggaca cgacattaac gatctgccgt accgttcagg tttcttccca
    17701 ctggggccga tcttcgcatt cattctgtgt ctgattatca ctttgggcca gaactacgaa
    17761 gcgttcctga aagataccat tgactggggc ggcgtagcgg caacgtatat tggtatcccg
    17821 ctgttcctga ttatttggtt cggctacaag ctgattaaag gaactcactt cgtacgctac
    17881 agcgaaatga agttcccgca gaacgataag aaataagttt tctcccttcc ttgctaagcc
    17941 ctctcaaccg agagggcttt ttcaattcca tttccctgac aaatcatgcg gatataaaat
    18001 ttaacatttg gattgataat tgttatcgtt tgcattatcg ttacgacgca atcaaaaaag
    18061 gctgacaaat cagaggctgt tccggctttc tgggatgatc acctgcatac aaataagtcc
    18121 accgcgatgc tgccgtacgc aaggggacgt aaagaagatg tgagcgatac ccattttatt
    18181 ttcgtagtta cctcatggag atatggaatg tttaggttga accctttcgt acgggtcggg
    18241 ctgtgtttgt ccgctatttc ttgtgcatgg cctgtgttag cggtcgatga tgatggcgaa
    18301 acgatggttg tcactgcatc ttccgtggaa caaaatctta aagatgcacc cgccagtatc
    18361 agcgtcatta cccaggaaga cctgcagcga aaaccggtac agaatctgaa ggatgtcctc
    18421 aaagaagtgc ctggcgtaca actgacgaac gaaggggata accgtaaggg cgttagtatt
    18481 cgtggtctgg acagcagcta caccctgatt cttgtcgacg gtaaacgcgt taactcccgc
    18541 aatgccgtct tccgccacaa tgatttcgat ctgaactgga tcccggtcga ttccatcgaa
    18601 cgtattgaag tggttcgtgg cccgatgtcg tcgctatacg gctccgatgc gctcggcggt
    18661 gtagtgaata tcatcaccaa aaaaatcggt cagaaatggt cgggtaccgt taccgtcgat
    18721 accaccattc aggaacatcg cgatcgcggt gatacctata acggtcagtt ctttaccagt
    18781 ggaccattaa ttgatggtgt gctgggaatg aaagcttacg gcagcctggc aaaacgtgaa
    18841 aaggatgacc cgcaaaactc aacgaccacc gataccggag aaacgccgcg tattgaagga
    18901 ttctccagcc gcgacggcaa tgtcgaattt gcctggacac cgaatcaaaa tcacgatttt
    18961 actgccggat acggtttcga ccgtcaggat cgtgattccg actagctgga caaaaaccgc
    19021 ctggaacgcc agaactactg cgtcagccat aatgggcgtt gggattacgg caccagcgaa
    19081 ctgaaatcct acggtgagaa agtcgagaac aaaaaccctg gcaacagcag cccgataact
    19141 tccgaaagca atacggtcga cggcaaatac acgttgccgc tgacggcgat taatcagttt
    19201 ctcacggttg gcggtgaatg gcgtcacgac aaacttagcg atgcggtgaa cctgaccggg
    19261 ggaaccagct ccaaaacgtc tgccagccag tacgcgctgt ttgtggaaga tgaatggcgg
    19321 atcttcgagc cgctggcgct gacgaccggc gtgcgtatgg acgatcacga aacctacggt
    19381 gaacactgga gtccgcgtgc ctacctggtt tataacgcca ccgacaccgt aacggtgaaa
    19441 gggggctggg cgacggcatt taaagcacct tctctgttgc aacttagccc tgactggacg
    19501 agcaattcct gccgtggcgc atgtaagatt gtgggtagcc cggatctgaa accagaaacc
    19561 agcgaaagtt gggagctggg gctttactac atgggtgaag aaggctggct ggaagggggt
    19621 gaatccagcg ttatcgtttt ccgtaacgat gtgaaagatc gtatcagcat cagccgtacg
    19681 tctgacgtca acgctgcacc gggctaccaa aactttgttg gttttgagac gggcgctaac
    19741 ggacggcgca taccggtatt tagctactac agcgttaaca aagctcgtat tcagggcgtg
    19801 gaaaccgaac tgaaaattcc gttcaacgat gaatggaaac tgtcgatcaa ctacacctac
    19861 aacgatggtc gtgatgtcag caacggcgaa aacaaaccgc tatccgatct gccgttccat
    19921 actgctaacg gtacgctgga ctggaaaccg ctggcgctgg aagactggtc attctatgtt
    19981 tctggtcact ataccgggca gaaacgcgcc gacagcgcga cggctaaaac accgggcggt
    20041 tataccatct ggaataccgg cgcggcctgg caggtgacta aagacgtcaa actgcgcgca
    20101 ggcgtgctga accttggcga caaggatctc agtcgtgacg actacagcta taacgaagac
    20161 ggacgtcgtt actttatggc agtggattat cgcttctgat gagaagatgc ccggcgaacc
    20221 gggcggactt tcacttcagt aaatactgcg catggaagcg caggtgatcc tctataaaag
    20281 aggcgatgaa gtagtaactg tgatcatatc ccggctgaat acgcagcgtc atcggccacg
    20341 ctttctggcg cgcggcttct gccaataccg caggttgcaa ctggtcggca agaaactgat
    20401 cattatcgcc ctgatcgata agcgtcggga tcgcatcctg cgcgttactg gcatacatca
    20461 gcgcgcaact atcccattcc agccatgcat ttttgtcctc acctaaatag gtgctaaacg
    20521 ctttgattcc ccacgggacg ctgcacggat tcacaatggg cgcaaaggcc gaaacgctgg
    20581 tgtatttacc cggatttttc agcgccataa tcagcgcacc gtgaccaccc attgagtgac
    20641 cgctaatggc gcagcggtcg ctgacattaa attgcgactg aaccagcgcc ggtaattcat
    20701 cgcgcagata atcatacatc cggtaatgcg tcgcccacgg cggttgcgtg gcattaagat
    20761 aaaagcctgc gccctggcct aaatcgtagc catcgtcgtt ggcaaccttt tcgccgcgcg
    20821 ggctggtgtc tggcatcacc agtacaatcc ccagttccgc cgctacccgc tgggcacccg
    20881 ccttggtggt gaagttctcg tcattgcagg ttaatccgga aagccagtac agcactggtg
    20941 gcggggtgtg atcacgaggg ggtgggagaa agatactgaa cgtcatcggg cagtttaagg
    21001 tactggagtc gtgtcgccag cgttgctgcc agccttcaaa acagcggtgc tcttcgagca
    21061 tttccatgcg tggctccttg ttgtgttgtt tgcaatttct tcatcatacc gtttattcaa
    21121 tgtgctgtga gtaactttca cttccgtatt tgcataacga tgttttaaca tctgctgatg
    21181 aaaggcagcg gcaattacaa taattatcgc tgtgaatact ggattatgtg cgccgcctca
    21241 cgcacaataa tcaagctgta aatcagctta ataactttgc ccccacgcag ggcggaggcg
    21301 tcacacctgc aggagaaatc ataaatgcca tcactcagta aagaagcggc cctggttcat
    21361 gaagcgttag ttgcgagagg actggaaaca ccgctgcgcc cgcccgtgca tgaaatggat
    21421 aacgaaacgc gcaaaagcct tattgctggt catatgaccg aaatcatgca gctgctgaat
    21481 ctcgacctgg ctgatgacag tttgatggaa acgccgcatc gcatcgctaa aatgtatgtc
    21541 gatgaaattt tctccggtct ggattacgcc aatttcccga aaatcaccct cattgaaaac
    21601 aaaatgaagg tcgatgaaat ggtcaccgtg cgcgatatca ctctgaccag tacctgtgaa
    21661 caccattttg ttaccatcga tggcaaagcg acggtggctt atatcccgaa agattcggtg
    21721 atcggtctgt caaaaattaa ccgtatcgtg cagttctttg cccagcgtcc gcaggtgcag
    21781 gaacgtctga cgcagcaaat tcttattgcg ctacaaacgc tgctgggcac caataacgtg
    21841 gctgtctcga tcgacgcggt gcattactgc gtgaaggcgc gtggcatccg cgatgcaacc
    21901 agtgccacga caacgacctc tcttggtgga ttgttcaaat ccagtcagaa tacgcgccac
    21961 gagtttctgc gcgctgtgcg ccatcacaac tgattaaaag gcaggaacca tggagcgcaa
    22021 cgtcacgctc gattttgttc gcggcgtcgc cattctgggg atcctgctat taaacatcag
    22081 cgcctttggg ctaccaaagg cggcttatct caatcccgcc tggtacgatg ctattacgcc
    22141 gcaggatgca tggacctggg catttctcga tctcatcggc caggtgaaat tcctcacgct
    22201 ttttgcgctg ctgtttggtg ctggcctgca aatgttgctg ccccgtggca gacgctggat
    22261 ccagtcgcgg ttaacgctgt tagtcttgtt gggctttatt cacggtttat tgttctggga
    22321 cggcgatatt ctgctggctt acgggctggt gggcttaatc tgctggtggc tggtgcgcga
    22381 tgcgccatcg gtaaaaagcc tgtttaatac cggcgtcatg ctttatctgg tggggcttgg
    22441 cgttttgctg ttattggggc tgatttccga cagccagacc agccgcgcct ggacgccgga
    22501 tgcatcggct attttgtatg aaaaatactg gaagcttcac ggcggcgttg aagcgatcag
    22561 taatcgtgcc gatggtgttg gcaatagttt actggcactg ggcgcacagt atggctggca
    22621 actggcaggg atgatgctca ttggtgcggc gctgatgcgc agcggctggc tgaaagggca
    22681 gttcagctta cgtcactatc gtcgtactgg ttttgtgctg gtggcgattg gggtgaccat
    22741 taaccttcct gccatcgccc tgcaatggca gctggactgg gcgtatcgct ggtgtgcctt
    22801 cttacttcaa atgccgcggg aactgagtgc gccgtttcag gcgattggct atgcgtcgct
    22861 gttttatggc ttctggccgc aattgagccg ctttaagctg gtgcttgcga tcgcctgcgt
    22921 cggacggatg gcgctgacca actatctatt gcaaacgctg atttgtacca cgctttttta
    22981 ccacctcggt ttgtttatgc attttgaccg cctggagctg ctggcgtttg ttattccggt
    23041 atggctggcg aatatcctct tctctgttat ctggctgcgt tacttccgcc aggggccggt
    23101 ggaatggctc tggcgtcagt taactttgcg tgctgccgga ctggcaatat ctaaaacatc
    23161 aagataacga tctggatcac attcgttaac aaaacggctg taaccgtttc cattgctgtg
    23221 actcgattca cgaagtcctg tattcagtgc tgacaaaata gccgccagca agcagtcatt
    23281 tactgcaatc tcataacagg tagtgaatat gatcaccatt cgtgatgtag cgcgtcaggc
    23341 tggcgtctct gtggcaacgg tttcccgggt gctcaataac agcacgctgg tcagtgccga
    23401 cacgcgtgaa gcagtaatga aagccgtgag tgagctggat tatcggccaa acgccaatgc
    23461 ccaggcgctg gcaactcagg ttagcgacac cattggcgtg gtggtgatgg acgtttctga
    23521 tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc gcccagcagc atcagaaata
    23581 cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag cgtcacgcca ttgaggtgtt
    23641 aattcgccag cgttgtaatg cgttgattgt tcactcaaaa gcattgagtg acgatgaact
    23701 ggcacaattt atggataaca ttcccggtat ggtgttaatc aaccgcgttg tgccggggta
    23761 cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc cgaatggcga cgcgcatgtt
    23821 gctgaataac ggtcatcaac gtattggtta tctttcttcc agccacggca ttgaagatga
    23881 cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag caggatatta ttccgccgga
    23941 aagctggatt ggcactggta cgccggacat gccgggcggt gaggcggcga tggttgaact
    24001 gctggggcgc aatctacaac ttaccgctgt atttgcttat aacgacaata tggccgctgg
    24061 cgcactgaca gcattaaaag ataatggcat tgcgattccg ttacatctct caatcatcgg
    24121 tttcgatgat attcccatcg cccgttacac cgacccgcaa ttaacgaccg tgcgttatcc
    24181 cattgcttca atggctaaat tagccaccga actggccttg cagggggcag caggcaatat
    24241 tgatcctcgt gccgccactg ttttatgccg acgttagtgc gtcgccattc tgtcgcaacg
    24301 cgccagaatg cggcggcgat cactaactca acaaatcagg cgatgtaacc gctttcaatc
    24361 tgtgagtgat ttcacagtat cttaacaatg tgatagctat gattgcaccg ttttaatgtt
    24421 gtaacccgta tgtaacagtg aataatcact ttcaccgagg taatagcgtc ataacaacaa
    24481 ttaaagccgt tttctggagc gttaccgggc atggaagaac gaattttaaa aagtgagctt
    24541 cggcgttcag taacacttca ttaactctac tgccccgccg agcatttatc tcaagcacta
    24601 ccctgcataa gaaaaaccgg agataccatg aataagaagg tgttaaccct gtctgctgtg
    24661 atggccagca tgttattcgg tgccgctgca cacgctgctg atactcgcat tggtgtaaca
    24721 atctataagt acgacgataa ctttatgtct gtagtgcgca aggctattga gcaagatgcg
    24781 aaagccgcgc cagatgttca gctgctgatg aatgattctc agaatgacca gtccaagcag
    24841 aacgatcaga tcgacgtatt gctggcgaaa ggggtgaagg cactggcaat caacctggtt
    24901 gacccggcag ctgcgggtac ggtgattgag aaagcgcgtg ggcaaaacgt gccggtggtt
    24961 ttcttcaaca aagaaccgtc tcgtaaggcg ctggatagct acgacaaagc ctactacgtt
    25021 ggcactgact ccaaagagtc cggcattatt cagggcgatt tgattgctaa acactgggcg
    25081 gcgaatcagg gttgggatct gaacaaagac ggtcagattc agttcgtact gctgaaaggt
    25141 gaaccgggcc atccggatgc agaagcacgt accacttacg tgattaaaga attgaacgat
    25201 aaaggcatca aaactgaaca gttacagtta gataccgcaa tgtgggacac cgctcaggcg
    25261 aaagataaga tggacgcctg gctgtctggc ccgaacgcca acaaaatcga agtggttatc
    25321 gccaacaacg atgcgatggc aatgggcgcg gtagaagcac tgaaagcaca caacaagtcc
    25381 agcattccgg tgtttggcgt cgatgcgctg ccagaagcgc tggcgctggt gaaatccggt
    25441 gcactggcgg gcacagtact gaacgatgct aacaaccagg cgaaagcgac ctttgatctg
    25501 gcgaaaaacc tggccgatgg caaaggtgcg gctgatggca ccaactggaa aatcgacaac
    25561 aaagtggtcc gcgtacctta tgttggcgta gataaagaca acttggctga attcagcaag
    25621 aaataagact gattgggcgc agtcgtttac tgcgccctta ttgtatggcc aacaaggtaa
    25681 aattatatgg tcagctcaac gactccgtct tccggggaat acttgttgga aatgagcggt
    25741 atcaacaagt cctttcctgg tgttaaggca cttgataacg ttaatttaaa agtccggcca
    25801 cattctatcc atgcattaat gggggaaaac ggtgcaggaa aatcgacatt attaaaatgc
    25861 ctgtttggta tttatcaaaa agactccggc accattttat tccagggtaa agagatcgat
    25921 ttccattccg ccaaagaggc actggaaaat ggtatttcga tggtacacca ggagttaaac
    25981 ctggtattac aacgttcggt gatggataac atgtggctgg ggcgatatcc caccaaaggc
    26041 atgtttgtcg atcaggacaa aatgtaccgc gaaaccaaag cgatttttga tgaactggat
    26101 attgatatcg atccgcgtgc gcgcgtcggc acattatccg tttcgcaaat gcagatgatc
    26161 gaaatcgcca aagcgttttc ctataacgcg aaaattgtga ttatggatga accgacttct
    26221 tcgttaaccg aaaaagaggt caatcatctg ttcactatta ttcgtaaatt aaaagagcgc
    26281 ggctgcggta ttgtttatat ctcgcataaa atggaagaaa tcttccagtt atgtgatgaa
    26341 gttaccgtat tgcgcgacgg tcagtggatc gccaccgaac cgctggcagg actgacgatg
    26401 gacaagatca tcggcatgat ggttgggcgt tctcttaatc agcgttttcc tgacaaagaa
    26461 aacaagccgg gcgaagtcat cctcgaggtg cgtaacctaa cgtcactgcg ccagccgtcg
    26521 attcgcgatg tctcgtttga tctgcataaa ggggagatcc tcggtattgc cgggctggtg
    26581 ggggcgaaac gtaccgatat tgttgagacg ttatttggta ttcgcgagaa atcggctggc
    26641 accattacgt tgcacggcaa acagatcaat aaccataatg ccaacgaagc cataaaccac
    26701 ggatttgcac tggtaactga ggagcgccgc tcaacgggaa tttatgccta tctggatatt
    26761 ggttttaact cgttaatttc caatattcgc aactacaaaa ataaagttgg tttactggat
    26821 aactcgcgga tgaaaagcga tacccagtgg gtgattgatt cgatgcgggt aaaaacgccg
    26881 ggtcatcgga cgcaaattgg ttcgctctcc ggtggtaatc agcaaaaggt gattattggt
    26941 cgctggctac taacgcaacc agaaatatta atgctcgatg aaccgacgcg cggtattgat
    27001 gtcggggcga agtttgaaat ttatcagtta attgccgaac tggcgaagaa aggcaagggg
    27061 attattatta tctcctctga aatgcctgag ttgttaggga taacagaccg tattctggtc
    27121 atgagcaatg gtctcgtttc cggaattgtc gatacaaaaa caacaacgca aaacgaaatt
    27181 ctgcgtcttg cgtctttgca cctttaagat taggggcttc ccatgagtgc gttaaataag
    27241 aaaagttttc ttacttacct gaaagagggc ggtatttacg tcgttctttt agttttgctg
    27301 gcgattatta ttttccagga cccaacattt ttaagtctgt tgaacttaag taatattctc
    27361 acccagtcat cggtgcgtat tattatcgcg ctcggtgtgg cagggttaat tgtcacccag
    27421 gggaccgatc tttctgctgg tcgtcaggta gggctggcgg cagtggtggc tgcgacatta
    27481 ttgcagtcca tggataacgc caacaaagtg ttcccggaaa tggcgacgat gccgattgcg
    27541 ctggttattc tgattgtctg tgccattggt gcggtgatcg gtttgatcaa cggtctgatt
    27601 atcgcttatc tcaacgtgac gccgttcatt accacgctcg gcacgatgat catcgtctat
    27661 ggcatcaact cgctctatta cgactttgtc ggggcgtcgc caatttctgg ttttgacagt
    27721 ggcttctcta cctttgctca gggctttgtc gcgctgggga gtttccgtct ctcttacatc
    27781 accttctacg cgttgattgc ggtggcgttc gtctgggtgt tgtggaacaa aacccgcttc
    27841 ggtaagaaca tttttgccat tggcggtaac ccggaagcgg caaaagtatc tggtgtcaac
    27901 gtcggcctga acctgctgat gatctacgcg ttgtctggcg tgttctatgc ctttggcggg
    27961 atgttagaag ccggacgtat cggctctgcc accaacaacc tcggctttat gtatgagctg
    28021 gatgctatcg cggcgtgcgt ggtaggcggt gtatcgttca gcggcggtgt ggggacggtg
    28081 attggcgtgg tgaccggggt aattattttt accgtcatca actatggcct gacgtatatc
    28141 ggcgtaaacc catactggca gtacattatc aaaggggcga ttattatctt cgccgtagcg
    28201 ctggattcac tgaaatacgc gcgtaagaag tgatgtgatt gcggatattg ccggatgcgc
    28261 tgcatccggc aatcggtgcc aaatgccgga tgcgtctctg gttttacaac gttaccgggt
    28321 gttctttctc gcctttctta aacttcactt ccccgagctc aatacaaccc accgggcaga
    28381 cgtgaccaca aagcagacaa cccacacatt tctcggtatt acaatgcggt gtgcgggttt
    28441 tctcgctcca ttccatcgcc tggtgaccgc cgtcgtaaca ggaaatataa cagcgtccac
    28501 agccaacaca tttgtcaaga ttgatacggg gatagacaat ataactgcgg tctaaatctt
    28561 ccgccgggac aatattgtta ttcgccagac ctaccatctc ctgcaacgaa tcaaatccct
    28621 gatcggcgag ataatgcgat aacccgctcg ccatatcttc tacgatgcga tagccgtact
    28681 gcataatgcc ggtggtgacc tgtaacgttg ctgcgccgag caataaaaac tccgccgcat
    28741 cttcccaggt ttcaatgccg ccgataccgc tgattgggaa atcgcgcagt tctggatggg
    28801 tgcgcatttg ctgaatgaag cgcagggcga tcggttttac cgctttaccg gaatatccgg
    28861 aaatactcga tttaccgtta acgatcggca taccgatttt ctgattaaga tcgatattgg
    28921 tgatggattt aacggtgtta attgccgcaa tgccatctgc gccgccgcgc ttcgccgcca
    28981 gcgccacttc gcacatatca ccgatattcg gcgtcatctt cgccagcatt ggcagcgtgg
    29041 aaccccgttt cactgcccga caatattttt ttaccagctc cgggctttgc ccgacatcgc
    29101 tacccatcgc atgagaagtc atttgcggac aggagaagtt acactcgatc atatccgcgc
    29161 cagcttcttg caccaggcgc gccagctcct cccattgctg ctcattttcc cccatgatcg
    29221 aagcgatcaa tactttgtcc gggtaatctt ccttcagccg acgcagggcg gtcagattct
    29281 cttccaacgg atgttcagca atctgctcca tatttttgaa gccgataaaa ccggtatctt
    29341 ctttcaccag atgatcaaaa cgcggcgaga cttcgttggc gataaaaaag ccgatcgttt
    29401 taaacaccac accgccccaa cctgtgtcgt aggctttggc acacatctca tagcagttgc
    29461 ctaccggcga agaagagagg cagaacgggt tgggaaagtt cacgccgcaa aaagtaatcg
    29521 aaagatcttt cgttaacatg agcaagctcc ctctaatata gtgatgaatc gcctcggcgg
    29581 cttctttccc ggttttcacg gcatagacca cggttttgtc accctcaaca atatcgccag
    29641 cagcaaagac ttgcggatcg cgggtctgat aattttgtgt tttgatggtg ttacgctgct
    29701 gctctaactc cgcaaaggca tccagtctgg catgctgacc gacggcgaga ataattttat
    29761 ctgccgccat cgtcagttcg cctgataacc gtacatgctt aaacgtgact ttattgcctt
    29821 ccacggctac tggcgtgaat ccatcgatga tcgaaacgcc cagctcccgg gcgctggcaa
    29881 actctttttc gcttgccgga aactcatcta actcttcacg tgctacgcaa gttaccgcct
    29941 gacagccgag aactttcagc gtgctggcta cgtccatcgc gacatcaccg ccgccgataa
    30001 ttaatgcgct ttgtggaatg ctgatatcgc cttgcggctg tcgtacacgt tgcaggaagt
    30061 cgacggcaat ctcaacggtc gctatgctca aacagcggta gcccggaacc gcttgataac
    30121 cccacggtga ccagtaccgc gcggttttcc gctttcagtt gctccagggt gagtgtttta
    30181 ccgatttcgt tattgcactt aatggtcacg cccatttttt caatacgggc gatctctgca
    30241 tccagcactg attgcggtaa ccggaattgc ggaataccgt tacgcaacca gccaccgggg
    30301 tgcgcttctt tctcataaat cgtgacgtca taaccctggt ttgtcagtgt cacactggcc
    30361 tgcaatccgg caggacctgc gccaataatc gcgactttgc cgagcgtttt agtaccgggc
    30421 tgataaattt ccatcccggt ttgttgttca aaatcagtaa caaaacgctg taagcggccg
    30481 atatcaatgg gagcatcaac accggcacgg gtacaaccgc tttggcataa tttctccgtc
    30541 gggcacactc tggcacaaac ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg
    30601 cctttaaaat tacgaaagta gattgagcga ataaatttcc ccggatcggt ctgtgccggg
    30661 caagcctgac tacagggagc gtcgtgacat aataaacagc gagaggcttc tttaatcgcc
    30721 agtaacggcg taaaagccgg agtgagttca tccagataat tttgttgcgg catgtgatct
    30781 tccttaatgc tatgagtaat tatggcgaac gctaaataaa tattgcgtgc tgatattaag
    30841 gctatgcgcg tggggaaatt taaactgtga aaggattcac aaatagctgc ggttcagaat
    30901 gttatatccg cagcttattg agtgaagtaa taattattct caataattaa cagaaaagag
    30961 aaaatattaa cgatagaaaa taagataaac aatcatgccg atagataaaa ttccgaagcc
    31021 aatatatgcc accatataag cgaagaagag tccgggattt tttgctcgcc agacatacac
    31081 cggttccggt gcatttttaa cccgtttatc aatcgcgcct gcgcgccagc ctttccatgc
    31141 ttcgcggaaa caaaatatcg ggataaggaa aaaaacggca acgacaaaaa actgctgtac
    31201 atccataact actttccttg tttcttttgt aattcaagta actgttcggg tgtaacggcg
    31261 ggatacccct gcgcatcttc cggcacctgg tgcatagccg gaatagggac cagcggccct
    31321 aaaaaacgcg gttcacgttt aaaaatataa aggtcagcca gcgcaccgaa acgggcggca
    31381 aattcgcgaa tacgtactga cagcatatct ttcggtgacg gtacggcata acactgagcc
    31441 tgaatcccca tatgcagcgc aataaataat gctcgctcac agtggaaacg ttgggtgata
    31501 ataatgaaat cattagtatc gaaaactttg cgtgtacgca cgatggaatc cagcgtacga
    31561 aagcctgcgt aatcgagaac aatatctgat gggtcgacac cagcagcgat taaatctttg
    31621 cgcatggtca tcggctcgtt ataactttgc aatgcgttat cgccgctcag taatagataa
    31681 tttaccttac cgctgttata ggcattaatc gctccttgaa tgcggtagcg ataatactga
    31741 ttaataacgc cggtacgata atattttgct gtgccgagca ccacgccgac ctggcggtag
    31801 gggagatcct gcaattcgtc gtagatataa ggcgcggttt tccagctcat ccagcgatcg
    31861 aggccgagca cagtcaacag cagcaagccg atcaggacta acaggctgag gaacacgcgc
    31921 tttaacatgc aggagactcg agctaaggaa aatttccatc aggctacttt acccgctggt
    31981 tttgcgcaag aaacggcaaa ctgtttgccg ggaatttcga caccaggaga gggctggcaa
    32041 gccggacaag gcgttcacgc cgcatccggc accaggctta agcgagaagc acgcggtcga
    32101 tactgtgaca gccgagagct ttcagcgttg cggaggtggc atcccactga atcaacggcg
    32161 catcggcttt ttctgccaga accgcgcgct ggatatccgg gtaatcataa cccttgagat
    32221 tcaacagaat taacgccccc tgcaacggcg gcagagtcgg gttgaatgcg gcgttttcag
    32281 cgtagctgcc gctgaaaata cgaccgtctt tacattccag cgcgacaccg cttggtgact
    32341 tactgtaagg catgtgcgaa cggtttgccg ctgcaatcgc cgcctgagaa agcgcatcac
    32401 cagtcagcgc atagccgtga tcctgctcgt ccatcagcag cgttttaatc tccagatctt
    32461 tcggcccaaa ggcatccggc agatagtcac gcagcgcgtg tgcctcgcgg cccggcaaat
    32521 gaatacgcag atccagaccg ctgttcagtt cattcataaa ctgacggcag tgaccacaag
    32581 gcgtatagtt aacggtgatg gctgcaagcg ctttttcacc actcaaccag gcgtggctga
    32641 tcgcgctttg ttcggcatga acggtttgct gcattgttgc accaataaat tccatattgg
    32701 caccaaaata ccaggttccg ctcacaccgc gcgcaattgc gccaacatta aaattcgaca
    32761 atggcgtacg cgcacaggcc gccgccagcg gaagtagtgc gaatgccagc gcgtcttcgt
    32821 ccagccccgt tgcgctcttc agcgatgaga cttgctcccc ggtcaacaaa gcggggaagt
    32881 acttgtctgc cagaataggt tccagtgcag attgcaagtt atccgcaagt tgggcaaaag
    32941 cggtttgaaa acgtggatgc atggcgttgc ctcataatca tgtaatggga taacagttta
    33001 cgaacccgtt atggctttat atgtgatctg aatctcatta attatgcaac acgtgaaact
    33061 tacatcaaaa atgcgcgacg tatcgcaaat tttagcccat tactgccaga ataatcggga
    33121 aaaggaacgg tgcgatcagc gaagtaatta tcccgcataa caccagcgct agcgaactaa
    33181 atgcaccttc ctgataatcc agctcggcgc agcgcgccgt accgagggcg tgcgaggcag
    33241 tccccattgc cagaccgcgc gcagctttgg tacgaatacg catcgcatta agcaatgtat
    33301 ggccaaatac cgcgccgagg atgccgacga aaatcacgca aactgcgctg attgccggaa
    33361 taccgccaat actgccgcca accgccattg caataggcgt ggtgactgat ttcggcagga
    33421 ttgacgcggc gatttccggt gaagcgccca tcaacaatgc cacggaagtc cccgtcacca
    33481 tcgcaaccac gctgccgata aaacagatgg tgatgatcga tttccagcgc gcgcggatct
    33541 ggtgtagctg ctcatataaa ggataggcca gcgcgacgac cgccggttgc agcagatcgt
    33601 ttagcacttc gctaccttta aagtaactgt cgtaagagat gccggtcagc attaaaaaag
    33661 gaatgatcac caccattgct accagcaacg ggttaagcag gggaaactta taccgtgccg
    33721 ccagtttgcg ggcggcaaaa aagacaatca acgtcagcgg taatgaccac cagatattcg
    33781 ccatcattct tctgatcctt tctgacctac gactttacgt tcaccgtgta ccagttgtga
    33841 actccagctc accaccagaa aaaccaccag cgtactgaca gcacaggaaa ccactaccgg
    33901 gccaaactgc gcgcggagca aatcaaaata ttgcatgacg cctacgccaa tcggcacaaa
    33961 caatagcgcc atatagcgaa tcagtacgta gcaccccgga ttgacccatt tcgccggaag
    34021 aatttgcaag gccagcagga caaacaggat cagcatcccg atgatgctgc ccggaatggt
    34081 taccggtagc agggaagcaa tgaaaatgcc tgcatacagg caggcataaa tcaggacgaa
    34141 agcgcgtaaa tattgccaga taatgttcag tgtcttactc ataatcaaca ccccagatga
    34201 gatggattca tcatacaatt aattcgtaaa acgtgctacc gatcacatta agaaatcagc
    34261 gcttattttt tcgctacccg gtattacgca atgagcagcg aactcaggca tacatcaagc
    34321 atagagaggg attgcagttt gcagcaatcc ctctttgcag acctttaatc cgctgaccat
    34381 aggcgctaac atagcgcctc atttttttgc gggtgatgat atacgtgtgt tactggcacc
    34441 gatggaggga gtgcttgact ctctggtgcg tgaattgctg accgaagtta acgactacga
    34501 tctgtgcatc accgagtttg tccgcgtggt ggatcaactg ctgccggtaa aagtctttca
    34561 tcgcatttgc cctgagctac aaaacgccag ccggacacca tctggtacgc tggtgcgcgt
    34621 gcagttgtta ggtcagttcc cacaatggct ggcagagaac gccgcccgtg cggtggagtt
    34681 aggttcctgg ggcgtggatc tcaattgcgg ctgcccgtcg aaaacggtta acggtagcgg
    34741 cggcggggcg acgttactca aagatcctga actcatctac cagggggcaa aagcgatgcg
    34801 tgaagctgta ccggcgcatt tgcctgtcag cgtgaaagtg cgtctgggct gggacagcgg
    34861 tgagaagaaa tttgaaatcg ccgatgcggt tcaacaggct ggcgctacgg agctggtggt
    34921 gcatgggcgg acgaaagagc agggttaccg cgcggagcat attgactggc aggcgattgg
    34981 cgagattcgc cagcggctga atattccggt gattgccaac ggtgaaatct gggactggca
    35041 gagcgcgcaa caatgcatgg cgatcagcgg ctgcgacgca gtgatgattg gtcgcggggc
    35101 gctcaatatt cccaacctga gccgggtggt aaaatataac gaaccgcgaa tgccgtggcc
    35161 ggaggtggtt gctttgctgc aaaaatatac ccgtctggaa aagcagggcg ataccgggtt
    35221 atatcacgtt gcgcggatta aacagtggtt gagttatttg cgtaaagaat acgatgaagc
    35281 aacggaatta tttcagcatg ttcg
//
LOCUS       DAJTYF010000028        35260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTYF010000028 DAJTYF010000000
VERSION     DAJTYF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.028"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            121..1149
                     /gene="murB"
                     /locus_tag="OO590_001511"
     CDS             121..1149
                     /gene="murB"
                     /locus_tag="OO590_001511"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6636921.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            1146..2111
                     /gene="birA"
                     /locus_tag="OO590_001512"
     CDS             1146..2111
                     /gene="birA"
                     /locus_tag="OO590_001512"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6636922.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2140..3090)
                     /gene="coaA"
                     /locus_tag="OO590_001513"
                     /gene_synonym="panK"
     CDS             complement(2140..3090)
                     /gene="coaA"
                     /locus_tag="OO590_001513"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6636923.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            3452..3527
                     /locus_tag="OO590_001514"
     tRNA            3452..3527
                     /locus_tag="OO590_001514"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3485..3487,aa:Thr,seq:tgt)
     gene            3536..3620
                     /locus_tag="OO590_001515"
     tRNA            3536..3620
                     /locus_tag="OO590_001515"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3570..3572,aa:Tyr,seq:gta)
     gene            3737..3811
                     /locus_tag="OO590_001516"
     tRNA            3737..3811
                     /locus_tag="OO590_001516"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3770..3772,aa:Gly,seq:tcc)
     gene            3818..3893
                     /locus_tag="OO590_001517"
     tRNA            3818..3893
                     /locus_tag="OO590_001517"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3851..3853,aa:Thr,seq:ggt)
     gene            4008..5192
                     /gene="tuf"
                     /locus_tag="OO590_001518"
     CDS             4008..5192
                     /gene="tuf"
                     /locus_tag="OO590_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6636924.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LS"
     gene            5422..5805
                     /gene="secE"
                     /locus_tag="OO590_001519"
     CDS             5422..5805
                     /gene="secE"
                     /locus_tag="OO590_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6636925.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            5807..6352
                     /gene="nusG"
                     /locus_tag="OO590_001520"
     CDS             5807..6352
                     /gene="nusG"
                     /locus_tag="OO590_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6636926.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            6511..6939
                     /gene="rplK"
                     /locus_tag="OO590_001521"
     CDS             6511..6939
                     /gene="rplK"
                     /locus_tag="OO590_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6636927.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            6943..7647
                     /gene="rplA"
                     /locus_tag="OO590_001522"
     CDS             6943..7647
                     /gene="rplA"
                     /locus_tag="OO590_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6636928.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            8060..8557
                     /gene="rplJ"
                     /locus_tag="OO590_001523"
     CDS             8060..8557
                     /gene="rplJ"
                     /locus_tag="OO590_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6636929.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            8624..8989
                     /gene="rplL"
                     /locus_tag="OO590_001524"
     CDS             8624..8989
                     /gene="rplL"
                     /locus_tag="OO590_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6636930.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            9309..13337
                     /gene="rpoB"
                     /locus_tag="OO590_001525"
     CDS             9309..13337
                     /gene="rpoB"
                     /locus_tag="OO590_001525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6636931.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGELVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            13414..17637
                     /gene="rpoC"
                     /locus_tag="OO590_001526"
     CDS             13414..17637
                     /gene="rpoC"
                     /locus_tag="OO590_001526"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6636932.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(17879..19012)
                     /gene="thiH"
                     /locus_tag="OO590_001527"
     CDS             complement(17879..19012)
                     /gene="thiH"
                     /locus_tag="OO590_001527"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6636933.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(19009..19779)
                     /gene="thiG"
                     /locus_tag="OO590_001528"
     CDS             complement(19009..19779)
                     /gene="thiG"
                     /locus_tag="OO590_001528"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6636934.1"
                     /translation="MLRIVDKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(19781..19981)
                     /gene="thiS"
                     /locus_tag="OO590_001529"
     CDS             complement(19781..19981)
                     /gene="thiS"
                     /locus_tag="OO590_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6636935.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(19965..20720)
                     /gene="thiF"
                     /locus_tag="OO590_001530"
     CDS             complement(19965..20720)
                     /gene="thiF"
                     /locus_tag="OO590_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6636936.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTADINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(20713..21348)
                     /gene="thiE"
                     /locus_tag="OO590_001531"
     CDS             complement(20713..21348)
                     /gene="thiE"
                     /locus_tag="OO590_001531"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6636937.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(21348..23243)
                     /gene="thiC"
                     /locus_tag="OO590_001532"
     CDS             complement(21348..23243)
                     /gene="thiC"
                     /locus_tag="OO590_001532"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6636938.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIHRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(23324..23424)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23477..23953)
                     /gene="rsd"
                     /locus_tag="OO590_001533"
     CDS             complement(23477..23953)
                     /gene="rsd"
                     /locus_tag="OO590_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6636939.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            24048..24821
                     /gene="nudC"
                     /locus_tag="OO590_001534"
     CDS             24048..24821
                     /gene="nudC"
                     /locus_tag="OO590_001534"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6636940.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            24861..25925
                     /gene="hemE"
                     /locus_tag="OO590_001535"
     CDS             24861..25925
                     /gene="hemE"
                     /locus_tag="OO590_001535"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6636941.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            26082..26753
                     /gene="nfi"
                     /locus_tag="OO590_001536"
     CDS             26082..26753
                     /gene="nfi"
                     /locus_tag="OO590_001536"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6636942.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            26796..27386
                     /locus_tag="OO590_001537"
     CDS             26796..27386
                     /locus_tag="OO590_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6636943.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            27573..27845
                     /gene="hupA"
                     /locus_tag="OO590_001538"
     CDS             27573..27845
                     /gene="hupA"
                     /locus_tag="OO590_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6636944.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            27858..28553
                     /locus_tag="OO590_001539"
     CDS             27858..28553
                     /locus_tag="OO590_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6636945.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRASFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(28555..28980)
                     /gene="zraP"
                     /locus_tag="OO590_001540"
     CDS             complement(28555..28980)
                     /gene="zraP"
                     /locus_tag="OO590_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6636946.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            29218..30594
                     /gene="zraS"
                     /locus_tag="OO590_001541"
     CDS             29218..30594
                     /gene="zraS"
                     /locus_tag="OO590_001541"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6636947.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            30591..31916
                     /gene="zraR"
                     /locus_tag="OO590_001542"
     CDS             30591..31916
                     /gene="zraR"
                     /locus_tag="OO590_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6636948.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(31913..33202)
                     /gene="purD"
                     /locus_tag="OO590_001543"
     CDS             complement(31913..33202)
                     /gene="purD"
                     /locus_tag="OO590_001543"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6636949.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(33214..34803)
                     /gene="purH"
                     /locus_tag="OO590_001544"
     CDS             complement(33214..34803)
                     /gene="purH"
                     /locus_tag="OO590_001544"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6636950.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         8412 a         9018 c         9600 g         8230 t
ORIGIN      
        1 aaggctatcc gtcaggatgg cctttttgcg tttctacaaa ctcttcctgt cgtcatatct
       61 acaagccatc cccccacaga tacggtaaac tagcctcgtt tttgcatcag gaaagcagct
      121 atgaaccact ccttaaaacc ctggaacaca tttggcattg atcataatgc tcagcacatt
      181 gtatgtgccg aagacgaaca acaattactc aatgcctggc agcatgcaac cgcagaagga
      241 caacccgttc ttattctggg tgaaggaagt aatgtacttt ttctggaaga ctatcgcggc
      301 acggtgatca tcaaccggat caaaggtatc gaaattcatg atgaacctga tgcgtggtat
      361 ttacatgtag gagccggaga aaactggcat cgcctggtaa aatacacttt gcaggaaggt
      421 atgcctggtc tggaaaatct ggcattaatt cctggttgtg tcggctcatc acctatccag
      481 aatattggtg cttatggcgt agaattacag cgagtttgcg cttatgttga ttgtgttgaa
      541 ctggcgacag gcaagcaagt gcgcttaact gccaaagagt gccgttttgg ctatcgcgac
      601 agtattttta aacatgaata ccaggatcgc ttcgccattg tagccgtagg tctgcgtctg
      661 ccaaaagagt ggcaacctgt actaacgtat ggtgacttaa ctcgtctgga tcctacaaca
      721 gtaacgccac agcaagtatt taatgcggtg tgtcatatgc gcaccaccaa actccctgat
      781 ccaaaagtga atggcaatgc cggtagtttc ttcaaaaacc ctgttgtatc tgccgaaacg
      841 gctaaagcat tactggcgca atttccaaca gcaccaaatt acccccaggc gggtggttca
      901 gtaaaactgg cagcaggttg gcttattgat cagtgccagc taaaagggat gcaaatgggt
      961 ggggctgcgg tgcaccgtca acaggcgtta gttctcatta atgaagacaa tgcaaaaagc
     1021 gaagatgtgg tgcaactggc acaccatgta agacaaaaag tgggtgaaaa atttaatgtc
     1081 tggcttgagc ctgaagtccg ctttattggt gcatcaggtg aagtgagcgc agtggagaca
     1141 atttcatgaa ggataacacc gtgccactga aattgattgc cctgttagcg aacggtgaat
     1201 ttcactctgg cgagcagttg ggtgaaacgc tgggaatgag ccgggcggct attaataaac
     1261 acattcagac actgcgtgac tggggcgttg atgtctttac cgttccgggt aaaggataca
     1321 gcctgcctga gcccatccag ttacttaatg ctgaacagat attgggtcag ctggatggcg
     1381 gtagtgtagc cgtgctgcca gttattgact ccacgaatca gttccttctt gatcgtatcg
     1441 gagagcttaa atcgggcgat gcctgtgttg cagaatacca gcaggctggc cgtggtcgcc
     1501 gggggcggaa atggttttcg ccttttggcg caaacttata tttgtcgatg ttctggcgtc
     1561 tggaacaagg cccggcggcg gcgattggtt taagtctggt tatcggtatc gtgatggcgg
     1621 aagtattacg aaagctggga gcagataaag ttcgtgtcaa atggcctaat gacctctatc
     1681 tgcaggatcg caagctggca ggcattcttg tggagctgac tggcaaaact ggcgatgcgg
     1741 cgcaaatagt cattggatcc gggatcaaca tggcaatgcg ccgtgttgaa gagagtgtcg
     1801 ttaatcaggg gtggatcacg ctgcaggaag cggggatcaa tcttgatcgt aatacgttgg
     1861 cggccatgct aatacgtgaa ttacgtgctg cgttggaact cttcgaacaa gaaggattgg
     1921 caccttatct gtcgcgctgg gaaaagctgg ataattttat taatcgccca gtgaaactta
     1981 tcattggtga taaagaaata tttggcattt cacgcggaat agacaaacag ggggctttat
     2041 tacttgagca ggatggaata ataaaaccct ggatgggcgg tgaaatatcc ctgcgtagtg
     2101 cagaaaaata agaaagggga gtattcgctc ccctgcaaat tatttgcgta gtctgacctc
     2161 ttctaccaca tgattagcac ttttcgtcag gattaaactg gcgcgctcac gagtaggtag
     2221 aatattttgc tttaagttca gccagttgat ctctttccac aatgtcatgg cagtcttaat
     2281 cgcttcttct ttagttaatt tcgcgtagtt atgaaaatag gaatccgggt ctgtaaaagc
     2341 cccttcgcgg aatttcagaa aacggttgat ataccacgtc tgaagtaagt cttccggtgc
     2401 atcaacatat atcgaaaaat cgacaaaatc ggaaacaaat acatgatgtg gatcgtgtgg
     2461 ataatccatc ccgctctgta agacatttaa cccttcaaga attaaaatat caggctgaac
     2521 aacagtttta tctccatccg ggatcacatc ataaataaga tgcgagtaaa caggggctgt
     2581 aacgtttggc acgccggatt tgagatcgga aacaaacttc accaggcgat gcatatcata
     2641 cgattccggg aagcctttct tcttcatcag accacgttct ttcagaacct gattagggtg
     2701 aaggaagcca tctgtagtga tcagttcaac acgacgatgt tccggccaac ggcttaatag
     2761 cgcctgcaat acacgggcgg ttgtactttt ccccaccgcg acactgccag caatactgat
     2821 aatgtaagga atgcgttgcc cgttggtacc aagaaactgt tccagaactg cctgacggcg
     2881 cagattcgag cttatataga agttcagcaa acgtgacaaa ggtaaataga tctcggcaac
     2941 ttcttctaac gagagatctt cattaatacc tttgagacgg gcgatctcat cttccgataa
     3001 cgtcataggt acggaatcac gcagagctgc ccactggttg cggtcaaact gtaggtaagg
     3061 cgtcattaac gtttgctctt ttatactcat aagcatgttt ctggcggtca tagctctatc
     3121 ggcaaagcga tatttgccat ggcgcgtcac agttctgttg aatgggttaa tgggcaggag
     3181 ggtaacacca gatggggagg aataataaga aaaaatctct gccaggaagc tatcgttgaa
     3241 aagcaatgtg acagatattc catctgataa aaagaattat ggtttagcag gagcgcattg
     3301 ttgagcacaa tgatgttgaa aaagtgtgct aatctgccct ccgttcggct gtttcttcat
     3361 cgtgtagcat aaaatgtgac caataaaaca aattatgcaa ttttttagtt gcatgaactc
     3421 gcatgtctcc atagaatgcg cgctacttga tgccgactta gctcagtagg tagagcaact
     3481 gacttgtaat cagtaggtca ccagttcgat tccggtagtc ggcaccatca agtccggtgg
     3541 ggttcccgag cggccaaagg gagcagactg taaatctgcc gtcacagact tcgaaggttc
     3601 gaatccttcc cccaccacca atttcggcca cgcgatggcg tagcccgaga cgataagttc
     3661 acttaccggc tcgaataaag agagcttctc tcgatattca gtgcagaatg aaaatcaggt
     3721 agccgagttc taggatgcgg gcatcgtata atggctatta cctcagcctt ccaagctgat
     3781 gatgcgggtt cgattcccgc tgcccgctcc aagatgtgct gatatagctc agttggtaga
     3841 gcgtaccctt ggtaagggtg aggtcggcag ttcgaatctg cctatcagca ccacttcttt
     3901 tctcctccct gttttttcct tctgtttatt gcattcaaca agtcgggcat gttgcctggt
     3961 tgatgtggtg atatcaccga tttatccgtg tcttagaggg acaatcgatg tctaaagaaa
     4021 agtttgaacg tacaaaaccg cacgttaacg tcggtactat cggccacgtt gaccatggta
     4081 aaacaacgct gaccgctgca atcactaccg tactggctaa aacctacggc ggtgctgctc
     4141 gcgcattcga ccagatcgat aacgcgccgg aagaaaaagc tcgtggtatc accatcaaca
     4201 cttctcacgt tgaatacgac accccgaccc gtcactacgc acacgtagac tgcccggggc
     4261 acgccgacta tgttaaaaac atgatcaccg gtgctgctca gatggacggc gcgatcctgg
     4321 tagttgctgc gactgacggc ccgatgccgc agactcgtga gcacatcctg ctggggcgtc
     4381 aggtaggcgt tccgtacatc atcgtgttcc tgaacaaatg cgacatggtt gatgacgaag
     4441 agctgctgga actggttgaa atggaagttc gtgaacttct gtctcagtac gacttcccgg
     4501 gcgacgacac tccgatcgtt cgtggttctg ctctgaaagc gctggaaggc gacgcagagt
     4561 gggaagcgaa aatcctggaa ctggctggct tcctggattc ttacattccg gaaccagagc
     4621 gtgcgattga caagccgttc ctgctgccga tcgaagacgt attctccatc tccggtcgtg
     4681 gtaccgttgt taccggtcgt gtagaacgcg gtatcatcaa agttggtgaa gaagttgaaa
     4741 tcgttggtat caaagagact cagaagtcta cctgtactgg cgttgaaatg ttccgcaaac
     4801 tgctggacga agggcgtgct ggtgagaacg taggtgttct gctgcgtggt atcaaacgtg
     4861 aagaaatcga acgtggtcag gtactggcta agccgggcac catcaagccg cacaccaagt
     4921 tcgaatctga agtgtacatt ctgtccaaag atgaaggcgg tcgtcatact ccgttcttca
     4981 aaggctaccg tccgcagttc tacttccgta ctactgacgt gactggtacc atcgaactgc
     5041 cggaaggcgt agagatggta atgccgggcg acaacatcaa aatggttgtt accctgatcc
     5101 acccgatcgc gatggacgac ggtctgcgtt tcgcaatccg tgaaggcggc cgtaccgttg
     5161 gcgcgggcgt tgtagcaaaa gttctgagct aattgccgat aacatttgac gcaatgcgca
     5221 ctaaaagggc atcatttgat gccctttttg cacgctttcg taccagaacc tggctcatca
     5281 gtgattttct ttgtcataat cattgctgag acaggctctg ttgagggcgt ataatccgaa
     5341 aagctaatac gcgtttcgat ttggtttgcc tcgcgatcgc ggggtgaaaa tgtttgtaga
     5401 aaacttctga caggttggtt tatgagtgcg aataccgaag ctcaaggaag cgggcgcggc
     5461 ctggaagcga tgaagtgggt cgttgtggtg gcattgctcc tggcggcgat tgtcggcaac
     5521 tacctttatc gcgacattat gctgccgctg cgtgcgctgg ccgtagtaat tctgattgct
     5581 gcagcgggtg gtgtcgcgct gttaacgaca aaaggtaaag ctaccgttgc ttttgcccgt
     5641 gaagcgcgta ccgaagtccg taaggtcatt tggccgactc gccaggaaac attgcacacc
     5701 acgctgattg tggctgcggt taccgcagta atgtcactga tcctgtgggg actggatggt
     5761 attctggttc gcctggtatc ctttatcact ggcctgaggt tctgagatgt ctgaagctcc
     5821 taaaaagcgc tggtacgtcg ttcaggcgtt ttccggtttt gaaggccgcg tagcaacgtc
     5881 gctgcgtgag catatcaaat tacacaacat ggaagatttg tttggtgaag tcatggtacc
     5941 aaccgaagaa gtggttgaaa tccgtggcgg tcagcgtcgc aaaagcgaac gtaaattctt
     6001 ccctggctac gtcctcgttc agatggtgat gaacgacgcg agctggcact tggtgcgcag
     6061 cgtaccgcgt gtgatgggct tcatcggcgg tacttccgat cgtcctgcgc caatcagcga
     6121 taaagaagtc gatgcgatta tgaaccgcct gcagcaggtt ggtgataagc cgcgtccgaa
     6181 aacgctgttt gaaccgggtg aaatggtccg tgttaatgat ggtccgttcg ctgacttcaa
     6241 cggtgttgtt gaagaagtgg attacgagaa atctcgtctg aaagtgtctg tttctatctt
     6301 cggtcgtgcg accccggtag agctggactt cagccaggtt gaaaaagcct aacccagcga
     6361 tcaaaaaagc ggcgatttaa tcgttgcaca aggcgtgaga ttggaataca atttcgcgcc
     6421 ttttgttttt atgggccttg cccgtaaaac gattttttat atcacgggga gcctctcaga
     6481 ggcgttatta cccaacttga ggaatttata atggctaaga aagtacaagc ctatgtcaag
     6541 ctgcaggttg cagctggtat ggctaacccg agtccgccag taggtccggc tctgggtcag
     6601 cagggcgtaa acatcatgga attctgcaaa gcgttcaacg caaaaactga ttccatcgaa
     6661 aaaggtctgc cgattccggt agtaatcacc gtttacgctg accgttcttt cactttcgtt
     6721 accaagaccc cgccggcagc agttctgctg aaaaaagcgg ctggtatcaa gtctggttcc
     6781 ggtaagccga acaaagacaa agtgggtaaa atttcccgcg ctcagctgca ggaaatcgcg
     6841 cagaccaaag ctgccgacat gactggtgcc gacattgaag cgatgactcg ctccatcgaa
     6901 ggtactgcac gttccatggg cctggtagtg gaggactaag aaatggctaa actgaccaag
     6961 cgcatgcgtg ttatccgcga gaaagttgat gcaaccaaac agtacgacat caacgaagct
     7021 atcgcactgc tgaaagagct ggcgactgct aaattcgtag aaagcgtgga cgtagctgtt
     7081 aacctcggca tcgacgctcg taaatctgac cagaacgtac gtggtgcaac tgtactgccg
     7141 cacggtactg gccgttccgt tcgcgtagcc gtatttaccc aaggtgcaaa cgctgaagct
     7201 gctaaagctg caggcgcaga actggtaggt atggaagatc tggctgacca gatcaagaaa
     7261 ggcgaaatga actttgacgt tgttattgct tctccggatg caatgcgcgt tgttggccag
     7321 ctgggccagg ttctgggtcc gcgcggcctg atgccaaacc cgaaagtggg tactgtaaca
     7381 ccgaacgttg ctgaagcggt taaaaacgct aaagctggcc aggttcgtta ccgtaacgac
     7441 aaaaacggca tcatccacac caccatcggt aaagtggact ttgacgctga caaactgaaa
     7501 gaaaacctgg aagctctgct ggttgcgctg aaaaaagcaa aaccgactca ggcgaaaggc
     7561 gtgtacatca agaaagttag catctccacc accatgggtg caggtgttgc agttgaccag
     7621 gctggcctga gcgcttctgt aaactaatgc ctttacgtgg gcggtgattt tgtctacaat
     7681 cttaccccca cgtataatgc ttaatgcaga cgtatatccg agatattcgg gttgtggcaa
     7741 ggcggcaact gagtgagtcg ccaggagcat agctaactat gtgactggtg cgaatgaagg
     7801 aagccaacgc cgccacaagt tgaatagcga cggatagaaa agatttgttc gttggagcct
     7861 ggcctatcca ggcctccgtc gaagaccgca ggagtttcgc aagaaactta atcccctgcg
     7921 tagacggtga cagaacgcta agattattct tttatattct ggcttgtttc tgctcaccgt
     7981 aattaagacg ctctctccgt ttggaggagt gaagtgagtt ccagagattt tctctggcaa
     8041 acatccagga gcaaagctaa tggctttaaa tcttcaagac aaacaagcga ttgttgctga
     8101 agtcagcgaa gtagccaaag gcgcgctgtc tgcagtagtt gcggattccc gtggcgtaac
     8161 tgtagataaa atgactgaac tgcgtaaagc aggtcgcgaa gctggcgtat acatgcgtgt
     8221 tgttcgtaac accctgctgc gccgtgctgt tgaaggtact ccgttcgagt gcctgaaaga
     8281 cgcgtttgtt ggtccgaccc tgattgcata ctctatggaa cacccgggcg ctgctgctcg
     8341 tctgttcaaa gagttcgcga aagcgaatgc aaaatttgag gtcaaagccg ctgcctttga
     8401 aggtgagctg atcccggcgt ctcagatcga ccgcctggca actctgccga cctacgaaga
     8461 agcaattgca cgcctgatgg caaccatgaa agaagcttcg gctggcaaac tggttcgtac
     8521 tctggctgct gtacgcgatg cgaaagaagc tgcttaatcg cagttatctt tttaacgcat
     8581 tcgcttacgt ataaacttat tctgatattc aggaacaatt taaatgtcta tcactaaaga
     8641 tcaaatcatt gaagcagttg cagctatgtc tgtaatggac gttgtagaac tgatctctgc
     8701 aatggaagaa aaattcggtg tttccgctgc tgctgctgta gctgtagctg ctggcccggt
     8761 tgaagctgct gaagaaaaaa ctgaattcga cgtaattctg aaagctgctg gcgctaacaa
     8821 agttgctgtt atcaaagcag tacgtggcgc aactggcctg ggtctgaaag aagctaaaga
     8881 cctggtagaa tctgcaccgg ctgctctgaa agaaggcgtg agcaaagacg acgcagaagc
     8941 actgaaaaaa gctctggaag aagctggcgc tgaagttgaa gttaaataag ccaacccttc
     9001 cggttgcagc ctgagaaatc aggctgatgg ctggtgactt tttagtcacc agcctttttg
     9061 cgctgtaagg cgccagtagc gtttcacact gtttgactac tgctgtgcct ttcaatgctt
     9121 gtttctatcg acgacttaat atactgcgac aggacgtccg ttctgtgtaa atcgcaatga
     9181 aatggtttaa gcgtgatagc aacaggcatt gcggaaagtg ttccattttc cggtcaacaa
     9241 aatagtgttg cacaaactgt ccgctcaatg gacagatggg tcgacttgtc agcgagctga
     9301 ggaaccctat ggtttactcc tataccgaga aaaaacgtat tcgtaaggat tttggtaaac
     9361 gtccacaagt tctggatgta ccttatctcc tttctatcca gcttgactcg tttcagaaat
     9421 ttatcgagca agatcctgaa gggcagtatg gtctggaagc tgctttccgt tccgtattcc
     9481 cgattcagag ctacagcggt aattccgagc tgcaatacgt cagctaccgc cttggcgaac
     9541 cggtgtttga cgtccaggaa tgtcaaatcc gtggcgtgac ctattccgca ccgctgcgcg
     9601 ttaaactgcg tctggtgatc tatgagcgcg aagcgccgga aggcaccgta aaagacatta
     9661 aagaacaaga agtctacatg ggcgaaattc cgctcatgac agacaacggt acctttgtta
     9721 tcaacggtac tgagcgtgtt atcgtttccc agctgcaccg tagtccgggc gtcttctttg
     9781 actccgacaa aggtaaaacc cactcttcgg gtaaagtgct gtataacgcg cgcatcatcc
     9841 cttaccgtgg ttcctggctg gacttcgaat tcgatccgaa ggacaacctg ttcgtacgta
     9901 tcgaccgtcg ccgtaaactg cctgcgacca tcattctgcg cgccctgaac tacaccacag
     9961 agcagatcct cgacctgttc tttgaaaaag ttatctttga aatccgtgat aacaagctgc
    10021 agatggaact ggtgccggaa cgcctgcgtg gtgaaaccgc atcttttgac atcgaagcta
    10081 acggtaaagt gtacgtagaa aaaggccgcc gtatcactgc gcgccacatt cgccagctgg
    10141 aaaaagacga cgtcaaactg atcgaagtcc cggttgagta catcgcaggt aaagtggttg
    10201 ctaaagacta tattgatgag tctaccggcg agctgatctg cgcagcgaac atggagctga
    10261 gcctggatct gctggctaag ctgagccagt ctggtcacaa gcgtatcgaa acgctgttca
    10321 ccaacgatct ggatcacggc ccatatatct ctgaaacctt acgtgtcgac ccaactaacg
    10381 accgtctgag cgcactggta gaaatctacc gcatgatgcg ccctggcgag ccgccgactc
    10441 gtgaagcagc tgaaagcctg ttcgagaacc tgttcttctc cgaagaccgt tatgacttgt
    10501 ctgcggttgg tcgtatgaag ttcaaccgtt ctctgctgcg cgaagaaatc gaaggttccg
    10561 gtattctgag caaagacgac atcattgatg ttatgaaaaa gctcatcgat atccgtaacg
    10621 gtaaaggcga agtcgatgat atcgaccacc tcggcaaccg tcgtatccgt tccgttggcg
    10681 aaatggcgga aaaccagttc cgcgttggcc tggtacgtgt agagcgtgcg gtgaaagagc
    10741 gtctgtctct gggcgatctg gataccctga tgcctcagga tatgatcaac gccaagccga
    10801 tttccgcagc agtgaaagag ttcttcggtt ccagccagct gtctcagttt atggaccaga
    10861 acaacccgct gtctgagatt acgcacaaac gtcgtatctc cgcactcggc ccaggcggtc
    10921 tgacccgtga acgtgcaggc ttcgaagttc gagacgtaca cccgactcac tacggtcgcg
    10981 tatgtccaat cgaaacccct gaaggtccga acatcggtct gatcaactct ctgtccgtgt
    11041 acgcacagac taacgaatac ggcttccttg agactccgta tcgtaaagtg accgacggtg
    11101 ttgtaactga cgaaattcac tacctgtctg ctatcgaaga aggcaactac gttatcgccc
    11161 aggcgaactc caacctggat gaaggaggcc acttcgtaga agacctggta acttgccgta
    11221 gcaaaggcga atccagcttg ttcagccgcg accaggttga ctacatggac gtatccaccc
    11281 agcaggtggt atccgtcggt gcgtccctga tcccgttcct ggaacacgat gacgccaacc
    11341 gtgcattgat gggtgcgaac atgcaacgtc aggccgttcc gactctgcgc gctgataagc
    11401 cgctggttgg tactggtatg gaacgtgctg ttgccgttga ctccggtgta actgcggtag
    11461 ctaaacgtgg tggtgtcgtt cagtacgtgg atgcttcccg tatcgttatc aaagttaacg
    11521 aagacgagat gtatccgggt gaagcaggta tcgacatcta caacctgacc aaatacaccc
    11581 gttctaacca gaacacctgt atcaaccaga tgccgtgtgt gtctctgggt gaactggttg
    11641 aacgtggcga cgtgctggca gacggtccgt ccaccgacct cggtgaactg gcgcttggtc
    11701 agaacatgcg cgtagcgttc atgccgtgga atggttacaa cttcgaagac tccatcctcg
    11761 tatccgagcg tgttgttcag gaagaccgtt tcaccaccat ccacattcag gaactggcgt
    11821 gtgtgtcccg tgacaccaag ctgggtccgg aagagatcac cgctgacatc ccgaacgtgg
    11881 gtgaagctgc gctctccaaa ctggatgaat ccggtatcgt ttacattggt gcggaagtga
    11941 ccggtggcga cattctggtt ggtaaggtaa cgccgaaagg tgaaactcag ctgaccccag
    12001 aagaaaaact gctgcgtgcg atcttcggtg agaaagcctc tgacgttaaa gactcttctc
    12061 tgcgcgtacc aaacggtgta tccggtacgg ttatcgacgt tcaggtcttt actcgcgatg
    12121 gcgtagaaaa agacaaacgt gcgctggaaa tcgaagaaat gcagctcaaa caggcgaaga
    12181 aagacctgtc tgaagaactg cagatcctcg aagcgggtct gttcagccgt atccgtgctg
    12241 tgctggtagc cggtggcgtt gaagctgaga agctcgacaa actgccgcgc gatcgctggc
    12301 tggagctggg cctgacagac gaagagaaac aaaatcagct ggaacagctg gctgagcagt
    12361 atgacgaact gaaacacgag ttcgagaaga aactcgaagc gaaacgccga aaaatcaccc
    12421 agggcgacga tctggcaccg ggcgtgctga agattgttaa ggtatatctg gcggttaaac
    12481 gccgtatcca gcctggtgac aagatggcag gtcgtcacgg taacaagggt gtaatttcta
    12541 agatcaaccc gatcgaagat atgccttacg atgaaaacgg tacgccggta gacatcgtac
    12601 tgaacccgct gggcgtaccg tctcgtatga acatcggtca gatcctcgaa acccacctgg
    12661 gtatggctgc gaaaggtatc ggcgacaaga tcaacgccat gctgaaacag cagcaagaag
    12721 tcgcgaaact gcgcgaattc atccagcgtg cgtacgatct gggcgctgac gttcgtcaga
    12781 aagttgacct gagtaccttc agcgatgaag aagttatgcg tctggctgaa aacctgcgca
    12841 aaggtatgcc aatcgcaacg ccggtgttcg acggtgcgaa agaagcagaa attaaagagc
    12901 tgctgaaact tggcgacctg ccgacttccg gtcagatccg cctgtacgat ggtcgcactg
    12961 gtgaacagtt cgagcgtccg gtaaccgttg gttacatgta catgctgaaa ctgaaccacc
    13021 tggtcgacga caagatgcac gcgcgttcca ccggttctta cagcctggtt actcagcagc
    13081 cgctgggtgg taaggcacag ttcggtggtc agcgtttcgg ggagatggaa gtgtgggcgc
    13141 tggaagcata cggcgcagca tacaccctgc aggaaatgct cactgttaag tctgatgacg
    13201 tgaacggtcg taccaagatg tataaaaaca tcgtggacgg caaccatcag atggagccgg
    13261 gcatgccaga atccttcaac gtattgttga aagagattcg ttcgctgggt atcaacatcg
    13321 aactggaaga cgagtaattc tcgctcaaac aggtcactgc tgtcggggta aaacccggca
    13381 gcggattgtg ctaactccga cgggagcaaa tccgtgaaag atttattaaa gtttctgaaa
    13441 gcgcagacta aaaccgaaga gtttgatgcg atcaaaattg ctctggcttc gccagacatg
    13501 atccgttcat ggtctttcgg tgaagttaaa aagccggaaa ccatcaacta ccgtacgttc
    13561 aaaccagaac gtgacggcct tttctgcgcc cgtatctttg ggccggtaaa agattacgag
    13621 tgcctgtgcg gtaagtacaa gcgcctgaaa caccgtggcg tcatctgtga gaagtgcggc
    13681 gttgaagtga cccagactaa agtacgccgt gagcgtatgg gccacatcga actggcttcc
    13741 ccgactgcgc acatctggtt cctgaaatcg ctgccgtccc gtatcggtct gctgctcgat
    13801 atgccgctgc gcgatatcga acgcgtactg tactttgaat cctatgtggt tatcgaaggc
    13861 ggtatgacca acctggaacg tcagcagatc ctgactgaag agcagtatct ggacgcgctg
    13921 gaagagttcg gtgacgaatt cgacgcgaag atgggtgcgg aagcaatcca ggctctgctg
    13981 aagagcatgg atctggagca agagtgcgaa cagctgcgtg aagagctgaa cgaaaccaac
    14041 tccgaaacta agcgtaaaaa gctgaccaag cgtatcaaac tgctggaagc gttcgttcag
    14101 tctggtaaca aaccagagtg gatgatcctg accgttctgc cggtactgcc gccagatctg
    14161 cgtccgctgg ttccgctgga tggtggtcgt ttcgcgactt ctgacctgaa cgatctgtat
    14221 cgtcgcgtca ttaaccgtaa caaccgtctg aaacgtctgc tggatctggc tgcgccggac
    14281 atcatcgtac gtaacgaaaa acgtatgctg caggaagcgg tagacgccct gctggataac
    14341 ggtcgtcgcg gtcgtgcgat caccggttct aacaagcgtc ctctgaaatc tttggccgac
    14401 atgatcaaag gtaaacaggg tcgtttccgt cagaacctgc tcggtaagcg tgttgactac
    14461 tccggtcgtt ctgtaatcac cgtaggtcca tacctgcgtc tgcatcagtg cggtctgccg
    14521 aagaaaatgg cgctggagct gttcaaaccg ttcatctacg gcaagctgga actgcgtggt
    14581 cttgctacca ccattaaagc tgcgaagaaa atggttgagc gcgaagaagc tgtcgtttgg
    14641 gatatcctgg acgaagttat ccgcgaacac ccggtactgc tgaaccgtgc accgactctg
    14701 caccgtctgg gtatccaggc atttgaaccg gtactgatcg aaggtaaagc tatccagctg
    14761 cacccgctgg tttgtgcggc atataacgcc gacttcgatg gtgaccagat ggctgttcac
    14821 gtaccgctga cgctggaagc ccagctggaa gcgcgtgcgc tgatgatgtc taccaacaac
    14881 atcctgtccc cggcgaacgg cgaaccaatc atcgttccgt ctcaggacgt tgtactgggt
    14941 ctgtactaca tgacccgtga ctgtgttaac gccaaaggcg aaggcatggt gctgactggc
    15001 ccgaaagaag cagaacgtct gtatcgctct ggtctggctt ctctgcatgc gcgcgttaaa
    15061 gtgcgtatca ccgagtatga aaaagatgct aacggtgaat tagtagcgaa aaccagcctg
    15121 aaagacacga ctgttggccg tgccattctg tggatgattg taccgaaagg tctgccttac
    15181 tccatcgtca accaggcgct gggtaaaaaa gcaatctcca aaatgctgaa cacctgctac
    15241 cgcattctcg gtctgaaacc gaccgttatt tttgcggacc agatcatgta caccggcttc
    15301 gcctatgcag cgcgttctgg tgcatctgtt ggcatcgatg acatggtcat cccggagaag
    15361 aaacacgaaa tcatctccga ggcagaagca gaagttgctg aaattcagga gcagttccag
    15421 tctggtctgg taactgcggg cgaacgctac aacaaagtta tcgatatctg ggctgcggcg
    15481 aacgatcgtg tatccaaagc gatgatggat aacctgcaaa ctgaaaccgt tattaaccgt
    15541 gacggtcagg aagagaagca ggtttccttc aacagcatct acatgatggc cgactccggt
    15601 gcgcgtggtt ctgcggcaca gattcgtcag cttgctggta tgcgtggtct gatggcaaag
    15661 ccggatggct ccatcatcga aacgccaatc accgcgaact tccgtgaagg tctgaacgta
    15721 ctccagtact tcatctccac ccacggtgct cgtaaaggtc tggcggatac cgcactgaaa
    15781 actgcgaact ccggttacct gactcgtcgt ctggttgacg tggcgcagga cctggtggtt
    15841 accgaagacg attgtggtac ccatgaaggt atcatgatga ctccggttat cgagggtggt
    15901 gacgttaaag agccgctgcg cgatcgcgta ctgggtcgtg taactgctga agacgttctg
    15961 aagccgggta ctgctgatat cctcgttccg cgcaacacgc tgctgcacga acagtggtgt
    16021 gacctgctgg aagagaactc tgtcgacgcg gtaaaagtac gttctgttgt atcttgtgac
    16081 accgactttg gtgtatgtgc gcactgctac ggtcgtgacc tggcgcgtgg ccacatcatc
    16141 aacaaaggtg aagcaatcgg tgttatcgcg gcacagtcca tcggtgaacc gggtacacag
    16201 ctgaccatgc gtacgttcca catcggtggt gcggcatctc gtgcggctgc tgaatccagc
    16261 atccaggtga aaaacaaagg tagcatcaag ctcagcaacg tgaagtcggt tgtgaactcc
    16321 agcggtaaac tggttatcac ttcccgtaac accgaactga aactgatcga cgaattcggt
    16381 cgtaccaaag aaagctacaa agtgccttac ggtgcggtac tggcgaaagg cgatggcgaa
    16441 caggttgctg gcggcgaaac cgttgcaaac tgggacccgc acaccatgcc ggttatcacc
    16501 gaagtaagcg gttttgtacg ctttactgac atgatcgacg gccagaccat tactcgtcag
    16561 accgacgaac tgaccggtct gtcttcgctg gtggttctgg attccgcaga acgtaccgca
    16621 ggtggtaaag atctgcgtcc ggcactgaaa atcgttgatg ctcagggtaa cgacgttctg
    16681 atcccaggta ccgatatgcc tgcgcagtac ttcctgccgg gtaaagcgat tgttcagctg
    16741 gaagatggcg tacagatcag ctctggtgac accttggcgc gtattccgca ggaatccggc
    16801 ggtaccaagg acatcaccgg tggtctgccg cgcgttgcgg acctgttcga agctcgtcgt
    16861 ccgaaagaac cggcaatctt ggctgaaatc agcggtatcg tttccttcgg taaagaaacc
    16921 aaaggtaaac gtcgtctggt tatcaccccg gtagacggta gcgatccgta cgaagagatg
    16981 attccgaaat ggcgtcagct caacgtgttc gaaggtgaac gtgtagaacg tggtgacgta
    17041 atttccgacg gtccggaagc gccgcacgac attctgcgtc tgcgtggtgt tcatgctgtg
    17101 actcgttaca tcgttaacga agtacaggac gtataccgtc tgcagggcgt aaagattaac
    17161 gataaacaca tcgaagttat cgttcgtcag atgctgcgta aagctaccat cgttaacgcg
    17221 ggcagctccg acttcctgga aggcgaacag gttgaatact ctcgcgtcaa gatcgcaaac
    17281 cgcgaactgg aagcgaacgg caaagtgggt gcaacttact cccgcgatct gctgggtatc
    17341 accaaagcgt ctctggcaac cgagtccttc atctccgcgg catcgttcca ggagaccact
    17401 cgcgtgctga ccgaagcagc cgttgcgggc aaacgcgacg aactgcgcgg cctgaaagag
    17461 aacgttatcg tgggtcgtct gatcccggca ggtaccggtt acgcgtacca ccaggatcgt
    17521 atgcgtcgcc gtgctgcggg tgaagctccg gctgcaccgc aggtgactgc agaagacgca
    17581 tctgccagcc tggcagaact gctgaacgca ggtctgggcg gttctgataa cgagtaatcg
    17641 ttaatccgca aataacgtaa aaacccgctt cggcgggttt ttttatgggt gttattcaga
    17701 gggcaaattc ttcacggaaa atattaagaa aggcagggcg aaacgaggaa gaagcttttc
    17761 gccctggaca acattcctgc tgacggcact accataaaaa aacacaacgc tttcaacaac
    17821 cttgcgtttt tcatcttccg tagcggctcg gaataaattt acgttcttac attccgtctc
    17881 atagtctttg cgaggcgcgt cccagatagc tgtcccagtc tttccatacc ggctgcaaac
    17941 cctgagcggt taacgcggca gcaaccgctt ccggtctgcg atcgtcgtgc ggtgagaact
    18001 gttccaactc ggggtgatta tcggcatagc cacctggctg cgttttcgag aaggcgctga
    18061 cgttattgat cgccagcgga ataacgcgat cgcgaaacca cggtgattcc cgcgtggaga
    18121 gtgacagttc aatctccggt gcaagcagtc ggaaggcgca gatggtttgc actaactggc
    18181 gttcatccat aatcgacgca ggctcaatgc cgccagtaca cgggcgcagg cgcggaaagg
    18241 agacagagta acggctttgc cagtaatgct gttgcagcca tagcaaatgt tcggcaacca
    18301 tatagcagtc aacgcgccag ttgtcggaaa ggccaattag cgcgccgagg cctatcttat
    18361 caatccccgc acgccccagc cgatccggcg tttccagccg ccagaagaag tcctgttttt
    18421 tgcctttcaa atgatggcgg gtataagtcg cctcgtgata tgtctcctga taaaccatca
    18481 ccccatccag accaagttgc tttaactcgg cgtattccgt ctccgccagc ggttgcactt
    18541 ccatttgtag tgaagagaac tgttcacgca gggcggggag atgacgacga aagtaatcca
    18601 tccccacttt cgcctgatgt tcaccagtga ctaatagcag atgttcaaag cccatctccc
    18661 gtatagcggc gctttccctg gcaatatccg cttcatccag cgttttgcgc ttgatgcgat
    18721 tactcatgga aaatccgcag tacgtgcagt tgttagcgca aagattggaa agataaagcg
    18781 ggacgtagaa actaactgtg ttgccaaatc gctgacgggt cagacgctgc gcctgttggg
    18841 ccagttgttc cagatagcca ctggcggcag gcgataacag cgccatcatg tcgtcgcggg
    18901 tgagttgcga ggcatttagc gcccgctcta cgtcagcagc cgttttgccg ttgatacgca
    18961 ggcggatgtc gtcccagtcc agttgtcgcc agcgattgct gaaggttttc atgccgatgc
    19021 ctccagaaat ccggtcagcg ggctggtggc atgagcaaaa taactgcggc tgcccggtcc
    19081 ggactgacgt gccagcaggc ctgcttctac cgccagacga aatgccttcg ccatgttgac
    19141 gggatcgtcc gcgacggcaa tcgccgtatt cactaacacc gcgtcggccc ccatttccag
    19201 cgcctgcgcg gcatggctgg gaacgccgat gccagcatcg acaaccaccg gaactgtggc
    19261 ctgctggata ataatctcca gcatggcacg ggtttccagt ccctgattcg agccaatcgg
    19321 cgcgccgagc ggcatcaccg ctgcacagcc gacttcttcc agacgtttac acaataccgg
    19381 atcggccccg cagtaaggca gcacgacaaa tccctgttgt accagcgttt cggcggcttt
    19441 cagggtttcg atgggatcgg gcaacagcca gcgggcgtca gggtgaatct ccaattttag
    19501 ccagtttgtg cctaacgctt cacgtgccag atgggcggcg aaaatggctt cttccgctgt
    19561 tttcgccccg gaagtattcg gcagcagggc cacacccgcc gcgataagcg gttcgaggat
    19621 agcgtcgttg tgctggcgca agtcgacacg tttcatcgcc agtgtcacca gctggctgcc
    19681 ggaagcgcgg atcgcctcca ccatcagttg tgaagaagcg aatttccctg tgccggtaaa
    19741 cagatgtgaa tcaaacgttt tgtccacaat acgtaacatc tcaaccccct gcaataacct
    19801 gaaaaagcag gatctggtcg ccatcctgca cgatatgttg cgtccactgc tcacgcggga
    19861 cgatttgctg attaatcgcc agagccgcgc ccgcttgtcg ttggtcgagt tgctccagta
    19921 gttcgtgaac agtttgcccg gcggcgcact gcatcggttg atcgttaaac aggatctgca
    19981 ttgcttcctc cgcataccgg gcaaccactg gcgcggcgca acgccaggct gcgccactgg
    20041 ctcgacttac cgtcgaacag tcggagttct cccgcaggcg tctctatacc gcttaataac
    20101 ttaatggctt ccagtgcctg caaagtgccc atcaccccga ccaccgggcc aaccacgccc
    20161 gccgtgcggc agttgcgttc tggctgctgg ttatctggcc acaggcagcg gtaacacccc
    20221 tgctcccagg gcggtgtcag taccatcaac tgaccgccaa atccgaccgc gctggcggtg
    20281 ataagcggcg tgttgagtgc cacgcaggcg gcattaatct cctggcgagt cgccatattg
    20341 tcggtacagt cgagcaccac atcggcttgt gcaaccgcat ctttcaacgc ctcacccgtt
    20401 aaccgttgtt gtaatgctgt cagttgaatg tcgggattca actgtgtcag tcgctgttgg
    20461 ctgacctgcg atttcgggct gttgatatct gcagtggtaa agaggatttg tcgttgcaga
    20521 ttgcttaaat gcacatcgtc gtcatctgcc agtaccagcg tcccgacgcc agcccctgcc
    20581 aggtatagcg cagcaggtgt acccagcccg ccaagaccaa taatcagcac ctggctgtcg
    20641 agcagttttt gctgcccgtc aagagcgata tcgtcgagca gaatttggcg gctataacgc
    20701 ataaagtcac ggtcattcat cgccaactcc tgcaatttcc agcaactgtg ccgttgccaa
    20761 acgccagtct gcggcttgag taatggcgct gacgacggcg atactgccga cacccgttgc
    20821 tatcaccgca ggcgcgcgtg ccagactgat accgccaatc gccacggtgg gataatccgc
    20881 caggcgctca acatgccgtg ccaactgttc cagcccctgc ggtgcagaag gcatctgttt
    20941 ggtttgcgtc gggaacacat gtcccagcgc gatataagag gggcgtgctg ccagcgcgac
    21001 gtcgatttcc atatcgtcat gtgtcgaaac gcccagccgc aggcctgccg cgcggatggc
    21061 attgagatcg gtggcttgca aatcttcctg ccccaaatgg acgccatacg cctgatgctt
    21121 gatcgcgagc cgccagtaat cgttgataaa caatcgcgcg ttatagcggc ggcccagcgc
    21181 aattgccgcc acgacatcgg cttccacctc ttcatcgcgc cgatctttga tgcgtagctg
    21241 gagagtacgt acgcctgcat ccaacagacg ttcgatccac tgtacgctgt ccaccaccgg
    21301 gtacagtcct aaacgaaaag gtacaggagg aaaatcaggc tgatacatca cgcttcctcc
    21361 ttatgcaggt agatttctcc gcctctggca cggaagttct ccgacatatc cgccattccc
    21421 acttcaatgg tttgcgcggc ggcgtaatcg cgtacttcct ggctgatttt catcgagcag
    21481 aattttggcc cacacatgga gcaaaaatgg gcgactttgc cggactcttg gggcagggtt
    21541 tcatcgtgat aagcgcgggc ggtaaacggg tcgagggcca gattaaactg gtcttcccag
    21601 cgaaattcga agcgggcttt cgacatggcg ttatcgcgaa tttgcgcgcc cggatgccct
    21661 tttgccaggt cagcagcgtg ggcggcaatc ttataagtga taagcccctg cttaacatct
    21721 tctttattgg gcagacccag atgctctttt ggcgttacgt aacagagcat cgcgcagcca
    21781 aaccagccaa tcatcgccgc accaatcccc gacgtgaagt ggtcatagcc cggtgcaata
    21841 tcggtagtta gcggccccag agtgtaaaac ggcgcttcgt ggcagtgctc taactcctcg
    21901 gtcatattgc ggtggatcat ctgcatcggc acatggcctg ggccttcaat catcacctgt
    21961 acgtcatatt cccaggcgat tttggtcagt tcgcccagcg tatgcaattc ggcaaactgc
    22021 gcttcatcgt tggcgtcctg aatagaaccg gggcgcagac cgtcgcccag cgacagcgaa
    22081 acgtcataag cggcacagat ttcacaaatt tcgcggaagt gttgatagag gaaattttcc
    22141 tgatgatggg agaggcacca tttcgccata atcgaaccgc cgcgagagac gataccggtc
    22201 aggcgtttcg cggtcatcgg cacatagcgc agcagtacgc ccgcatggat agtgaagtaa
    22261 tccacacctt gctcggcctg ttccagcagc gtgtcgcgga acgcttccca ggtaagatct
    22321 tcggcgatcc cgttaacctt ctccagcgcc tggtagatcg gcactgtacc gatcggcacc
    22381 ggactgttac gcaaaatcca ctcgcgggtt tcgtgaatat agcgaccggt ggagagatcc
    22441 atcaccgtat ccgctcccca gcgcgtggac cataccagct tttccacttc ttcttcgatg
    22501 gaagaggtga ccgccgagtt gccgatattg gcgttaactt ttaccaggaa gttacggcca
    22561 ataatcatcg gctccgattc cggatgatta atgttggccg ggataatcgc acgtccggca
    22621 gcaacttcat cacggacaaa ttccgcagtg atattttccg gcagatgtgc gccaaagctc
    22681 attcccggat gctggtggcg taaaacctcg ctacggatgc gctcgcggcc catattctcg
    22741 cggatggcga tgaattccat ttccggcgtg atgatgccct ggcgggcgta gtgcagttgg
    22801 gtgacacggc gtcctgtttt ggcgcgtttt ggcgttaata agccgctaaa acgtagctcg
    22861 tcgagaccat catctgccag ccgcgttctg gtgtaatcgg aactgcgcac ggtaagttct
    22921 tcggtatcgg cgcgcgcatc gatccacggc tggcgtagtt ttgccagccc ttgctgcacg
    22981 ttaatggcga tctgcggatc accatacggg ccggaggtgt cgtagaccgg aatcgcttcg
    23041 ttttcttcgt actgcggctg ttctttgcta ccgccaatta gcgtcgggct aagctggatc
    23101 tcacgcatcg gcacgcgcac gccggggtgt gtgccagtga tataaatgcg ttttgagttg
    23161 ggaaaggcgg tgccttccag ggtgtcgata aaatgttggg cccgggcgcg ttgttcgcgg
    23221 cgggtcagtt ttgttgcaga catagctcat tccaaaaagt taaggacgtg gcttgtcaga
    23281 cgacggatga agcaagagac gatcgccgca aaggcgatgc gatagcagat taactcttgt
    23341 tcccttcgca ggtattagcc tgatcaggtt ccgcggatcc cgaataaacg gtctcagcca
    23401 gttaaggcac tccgacaaga aattagcccc cacaaacggg gcgttgaatg taaattacgc
    23461 gttaacagcg cagaactcaa gcaggatgtt tgacgcgggc ggcgtcaagc accagcagaa
    23521 tcagcttatc ttccagcaca aagcgcgctt ccagcgcctc gccgatgtca gataaaacct
    23581 gttgaaactc aaggtaatta tcatgatcga tggcggtttc caggctggaa tcgtagtaat
    23641 ccataatctg ttgggtattg gcttcgagtt gcggccaaat cttcgcggcg cgcgcgagtt
    23701 gcccgttgcc ttccagctta tgaagaatgc gctcataaat actgaaatgt ccggcagaca
    23761 agtaatcgac caggctctga caaaaatcat caagggcttt ttcgtttagc ctcatgtacg
    23821 attctttgcc aggcttaatg ccaaccagat tgtagtaagc cacgagcaga tgcttacgta
    23881 catgtagcca gcgatcaacc agtttgttac ttcctctgac gcgctccgtc aggttatcga
    23941 gctggttaag catgattgac tccgcaagtt tgtattcaaa aactgctcag tgagaaatgt
    24001 aaaaaccatg ttaaacatgc cagtgatgca aaggtagtgc aagagctatg gatcgtataa
    24061 ttgaaaaatt agatcacggc tggtgggtcg tcagccatga acaaaaatta tggttgccga
    24121 agggagaatt gccatatggc gaagcggcaa atttcgatct tgtgggtcag cgcgcactac
    24181 aaatcggcga atggcagggg gaacctgttt ggttaataca acagcagcgg cgtcacgata
    24241 tggggtcggt acgtcaggtc attgatctcg atgttgggct gtttcaactg gccggacgtg
    24301 gcgtgcaact ggcggagttt taccgatcgc ataaatactg tggttactgc gggcatgaaa
    24361 tgtatccgag caaaaccgaa tgggcgatgc tgtgcagcca ttgccgtgag cgttactacc
    24421 cgcaaatcgc cccctgcatt attgttgcca tccgtcgcga tgattcgatc ctcctcgccc
    24481 agcatacccg ccatcgtaac ggtgtccata cagtacttgc cggattcgtc gaagtgggcg
    24541 aaaccctcga gcaggcagtc gcgcgggaag tgatggaaga gagcggaatt aaagttaaaa
    24601 acttgcgtta cgtgacttct cagccgtggc cgtttcctca gtctttaatg accgcgttta
    24661 tggcggaata tgacagcggc gacatcgtga tcgacccgaa agaattgctc gaggcgaact
    24721 ggtatcgcta tgacgatttg ccgttactcc cgccgcccgg caccgtagcg cgccgtctga
    24781 tagaagatac tgtggcgatg tgtcgggcag agtatgagtg atgatacact gaccgcctga
    24841 cgcactaagg aacagccaaa atgaccgaac ttaaaaacga tcgttatctg cgggcgctgc
    24901 tgcgccagcc cgttgatgtc actccagtat ggatgatgcg ccaggcgggt cgctatctac
    24961 cggaatataa agccacgcgc gcccaggcgg gcgattttat gtcgctgtgc aaaaacgccg
    25021 agctggcgtg cgaagtgact ttgcaaccgc tgcgtcgcta cccgctggat gcggcgatcc
    25081 tcttttccga tatcctcacc gtgccggacg cgatggggtt agggctctat tttgaagccg
    25141 gagaaggtcc gcgttttacc tcgccagtca cctgcaaagc cgacgtcgat aaactgccaa
    25201 ttccggaccc ggaagatgag ctgggttacg tgatgaacgc ggtgcgtacc attcgtcacg
    25261 aactgaaagg cgaagtgccg ctgattggtt tttccggcag cccgtggacg ctggcaacct
    25321 acatggtgga aggcggcagc agcaaagcct tcaccgtgat caaaaaaatg atgtatgccg
    25381 atccgcaggc gctgcacgct ctgctcgata aactggcgaa aagcgtcact ttgtatctga
    25441 atgcgcagat taaagccggt gctcaggcag tgatgatttt cgacacctgg ggcggcgtgc
    25501 ttaccgggcg cgattatcaa cagttctcgc tctattacat gcataaaatt gttgatggtt
    25561 tactgcgtga aaacgacggt cgccgcgtgc cagtcacgct gtttaccaaa ggcggcggac
    25621 agtggctgga agccatggct gaaaccggtt gcgatgcgct gggcctcgac tggacaacgg
    25681 acatcgccga tgcgcgccgc cgtgtgggca ataaagtcgc gttgcagggt aatatggatc
    25741 cgtcgatgct gtacgcgccg cctgcccgca ttgaagaaga agtagcgact atacttgcag
    25801 gtttcggtca cggcgaaggt catgtcttta accttggtca cggcattcat caggatgtgc
    25861 cgccagaaca tgctggcgtg ttcgtggagg cagtgcatcg actgtctgaa cagtatcacc
    25921 gctaagagcc tgatccatca ggctatttta tttgccattt tgaggccggg cagtgctcaa
    25981 aatcctcacg tactacgtgt acgctccggt ttttccgcgc tgtccggcct caaactgcct
    26041 tcaacaatta cgcctgttga accaggctct aaggagcgat tatggatctc gcgtcattac
    26101 gcgctcaaca aatcgaactg gcttcttctg tgatctgcga ggatcgactc gataaagatc
    26161 caccggatct gatcgccgga gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag
    26221 cggcgatggt gctgctgaaa tatccctcgc ttgagctggt ggagtataaa gttgcccgca
    26281 tcgccaccac catgccttac attccaggtt ttctttcctt ccgcgaatat cctgcgctgc
    26341 tggcagcgtg ggagatgctg tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga
    26401 tctcgcatcc tcgccgtctt ggcgtcgcca gccattttgg cttattggtg gatgtgccga
    26461 ccattggcgt ggcgaaaaaa cggctctgcg gtaaattcga accgctctcc agcaaaccgg
    26521 gcgcgctggc cccactgatg gataaaggcg agcagctggc ctgggtctgg cgcagcaaag
    26581 cgcgctgtaa cccgttgttt atcgctaccg gccatcgggt cagcgtggac agcgcgctgg
    26641 cgtgggtaca acgctgcatg aaaggctatc gtctgccgga gccaacgcgc tgggcggacg
    26701 cggtggcctc ggaacgtccg gcgttcgtgc gctatacagc aaatcagccc taattcaggt
    26761 aaactgcgga taatttccgt atttgagaac tcaacatgtt acaaaaccca attcatctgc
    26821 gtctggagcg cctggaaagc tggcagcatg tcactttcat ggcttgctta tgcgaacgca
    26881 tgtaccccaa ttacgccatg ttctgccagc aaaccggttt tggtgatggg caaatttacc
    26941 gtcgtattct cgatctcatc tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg
    27001 acagccaact ggagaaattt gaagaagcga ttccttcagc cgacgatttc gatctgtacg
    27061 gcgtttatcc ggcaatcgat gcctgcgtgg cgttaagtga actggtccat tcgcgtttga
    27121 gtggtgaaac gctcgaacac gcggtggaag tgagtaagac ctccatcacg accgttgcga
    27181 tgctggaaat gactcaggct ggtcgcgaaa tgagcgatga agagcttaaa gaaaacccag
    27241 ctgtagagca agaatgggac attcagtggg aaatattccg acttttagct gagtgcgaag
    27301 aacgcgatat cgagctgata aaaggcctta gggcagacct gcgtgagtcg ggtgagagca
    27361 atattggtat aatttttcag caataagacc agaaaacgtg atttaacgcc tgatttgtcg
    27421 tacctggagt cttccctttc gccccccgtc tggtctacat ttggggggcg aaaaaaagtg
    27481 gctatcggtg cgtgtatgca ggagagtgct attctggcat ttccgtcgca ctcgatgctt
    27541 agcaagcgat aaacacattg taaggataac ttatgaacaa gactcaactg attgatgtaa
    27601 ttgcagagaa agcagaactg tccaaaaccc aggctaaagc tgctctggag tccactctgg
    27661 ctgcaattac tgagtctctg aaagaaggcg atgctgtaca actggttggt ttcggtacct
    27721 tcaaagtgaa ccatcgcgct gagcgtactg gccgcaaccc gcagaccggt aaagaaatca
    27781 aaattgccgc agctaacgta ccggcatttg tttctggcaa ggcactgaaa gacgcagtta
    27841 agtaagattg cgtggcagtg aacagtttta acgaaggggt ggtttcaccc cttttgtctt
    27901 tctggcgtcg atcattgatg ctggcaggtg cgctgtttct cactgcctgt agccacaact
    27961 cttcacttcc tccctttacc gccagtggat ttgctgaaga ccagggcgcg gtacgcatct
    28021 ggcgaaaaga cagcggcgat aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg
    28081 gcgataccac gacgcgagag tatcgctggc agggcgataa cctcacgctc atcaatatca
    28141 atgtttacag caaaccgccg gtgaacattc gcgcgagttt tgacgatcgc ggcgatctga
    28201 gctttatgca acgtgaatcc gatggggaaa agcagcagct ttctaacgac caaatcgatt
    28261 tataccgtta tcgtgctgat cagatccgcc agattagcga tgccttacgt caggggagag
    28321 tcgtgctgcg ccaggggcgc tggcatgcga tggaacagac cgtgaccacc tgcgaagggc
    28381 aaaccattaa acctgattta gattcgcagg cgatagcgca tatcgagcgc cgccagagcc
    28441 gctcttctgt tgatgtcagc gtggcatggc tggaagcgcc cgaaggttcg caattactgt
    28501 tagtggcaaa ctctgatttc tgtcgctggc aacccaacga gaaaacgttc tgatttacca
    28561 gtggcccata cccatatgac cgccaccacc gcagccgccg tagcccatgc ccattccggc
    28621 tccgcgcgga atacccgctt cagccatcgc gatatctcgt ttcacccgta actcatctaa
    28681 cgactgacgc aaattctcca tctctttggc gaccgcgtta attttgctgc tatccggtgg
    28741 gttcgcggct aacagggcat tgtattcata acgcttcgtc accagttgct gttgcagtgc
    28801 gctgctttga gcgtaaaagt cattatggat tttctgccac gctgtctgtt gttcgctggt
    28861 caaaggcgcg gcattttgct gccacatacc gtgtccgccg tgagcaaatg cagatgtcga
    28921 tcccatcgcc attgctgaaa gcgccatcat taccagggca attttcgtgt tccgtttcat
    28981 ggttaatcct ccagtggttg tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa
    29041 cgctgatatg acgggtaatc tggcatgata aacgagtaaa aatgactcgc ctgctgcggg
    29101 tagcgagtca tttttactca ttgaaactta agcctttgtg ttacagcgca gggtaagcgc
    29161 tgataaaaga tggcatgatt tctgctgtca gaaagggatg agcaggcaaa gaagaagatg
    29221 cgttttatgc aacgttctaa agactcctta gctaaatggt taagcgcgat cctccccgtg
    29281 gtcattgttg ggctggtagg gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag
    29341 actgccgccg ccagacaaac gctgctggaa aaaggcagtg tacttattcg cgctcttgaa
    29401 tccggctcgc gcgtcggcat ggggatgcgc atgcatcatg cgcagcagca ggccttactg
    29461 gaagaaatgg ccgggcagcc tggagtacgt tggtttgcgg tcacggatga acaaggaaca
    29521 atcgtgatgc atagcaactc cggcatggtg ggaaaacagc tttattcccc tcaggaaatg
    29581 cagcagttac atccgggaga tgaagaagcg tggcggcgga tcgatagcgc agacggcgag
    29641 cctgttctgg aaatttatcg ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg
    29701 cgccatatgc agcagtatgc cgcgacacca caagcaattt tcatcgcttt cgacgccagt
    29761 aatattgtga gtgccgaaga tcgtgagcag agaaacaccc tgattattct cttcgccctg
    29821 gcgacggtct tgctggcaag cgtattgtca ttcttctggt atcgccgcta tctgcgctcg
    29881 cgccagcttc tacaagatga aatgaagcgc aaagagaagc tggtggcgct ggggcatctt
    29941 gcggcaggcg ttgcccacga aatccgtaac ccactttcct cgattaaagg actggcgaaa
    30001 tactttgccg agcgcgcgcc tgcaggggga gaagcgcatc aactggcgca ggtgatggcg
    30061 aaagaggccg accgtttaaa ccgcgtggta agcgagttgc tggaactggt taagccaacg
    30121 catctggctt tgcaggcggt ggatctcaac acgctgatta accactcatt acagctggta
    30181 agccaggatg caaacagccg ggagatccag ttacgcttta ccgccaacga cacattaccg
    30241 gaaattcagg ccgatccgga caggctgact caggtcctgt tgaatctcta tctcaatgct
    30301 attcaggcga ttggtcagca tggcgtgatt agcgtgacgg ccagcgaaag cggcgcgggc
    30361 gtgaaaatca gcgttaccga cagcggtaag ggaattgcgg cagatcagct tgaagccatc
    30421 ttcactccgt acttcaccac caaagccgaa ggcaccggat tggggctggc ggtcgtgcat
    30481 aatattgttg aacaacacgg tggtacaatt caggtcgcaa gccaggaggg aaaaggctca
    30541 acgttcaccc tctggcttcc ggtcaatatt acgcgtaagg acccacaagg atgacgcacg
    30601 ataatatcga tattctggtg gtggatgatg acattagcca ctgcactatt ttgcaggctt
    30661 tactgcgcgg ctggggctat aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc
    30721 aggtgcggga acaggttttt gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg
    30781 gcatcgccac gctgaaagag atcaaagcgt taaacccggc aattccggtg ctgattatga
    30841 ctgcgtactc cagcgtcgag acggcggtag aggcactgaa aactggggcg ctggattatc
    30901 tcatcaagcc gctggatttc gataacctac aggcgacgct ggaaaaggcg ctcgcgcata
    30961 cgcacagtat tgatgctgaa acacctgcgg tgactgccag ccagttcggt atggtcggta
    31021 aaagcccggc gatgcaacac ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca
    31081 cggtgctgat ccacggcgat tccggcaccg gtaaagagct ggtcgccagg gcgattcacg
    31141 ccagtagcgc acgtagcgaa aaaccgctgg taacgctcaa ctgtgcggca ctcaacgaat
    31201 ccttgctgga atctgaattg ttcggtcacg aaaaaggggc gtttactgga gccgataaac
    31261 gccgggaagg gcgctttgtt gaggcggacg gcggcacgct gtttctcgat gaaattggcg
    31321 atatctcgcc gatgatgcag gtgcgtctgc tgcgtgcgat tcaggagcgc gaagttcagc
    31381 gtgtcggtag caaccagact atctcggttg atgtccggct gattgcggcg actcatcgcg
    31441 atcttgccgc agaggtgaat gccgggcgtt ttcgccagga tctctactat cgcctgaatg
    31501 tggtggcgat tgaagtacca tcgctgcggc aacggcggga agatatccct ctgctggctg
    31561 gccattttct tcagcgcttt gccgagcgta atcgcaaggc ggtaaaaggc tttacgccac
    31621 aggcgatgga tttgctgatc cactacgact ggccgggaaa tattcgtgaa ctggaaaacg
    31681 cggtggaacg ggcggtggtg ctgctgaccg gggaatatat ttccgaacgc gagctgccgc
    31741 tggcgattgc cagtacgccg atcccgctgg ggcaaagtca ggatattcag ccgctggtgg
    31801 aagtggaaaa agaggtgatt ctggcggcgc tggagaaaac gggcggcaac aaaaccgaag
    31861 ccgcccgtca gttagggatc acgcgcaaaa cgctattggc aaaactgtcg cgttagttct
    31921 gctcgcgttc gatggcgcgc caaccgatat ctttccggca gaagcaatca tcccagtgga
    31981 tatcggtcat taaggcgtag gcgcgtttct gcgcttctgc cacggtatga cccagcgcgg
    32041 tgacgcacag tacgcgcccg ccgctggtta ccacctgctc gtcatccgcc agttttgtgc
    32101 ccgcgtggaa cactttgccg tcagccactt cttccagcgg caggccgtgg atcacatcgc
    32161 cggtgcggta atcgcccgga tatccacccg cagccatcac cacgctgaga gaagcacgtt
    32221 catcccactc tgacgttttc tcgtccagtt tgccttcaca ggccgccagg cagagctcaa
    32281 ccagatcgga cttcatgcgc agcataatcg gctgggtttc cggatcgcca aagcggcagt
    32341 taaattcaat aaccttcgga ttgccctgtt tgtcgatcat caggcccgca tagagaaaac
    32401 cggtgtaggt gttgccttcc gccgccatgc ctttcacggt tggccagatg atacgttcca
    32461 tggtgcgctg atgaacttca tcggttacta ccggcgcggg ggagtaagcg cccatcccgc
    32521 cggtgtttgg tccgttatct ttatcgccta cgcgtttgtg atcctggctg gtagccatcg
    32581 gcagcacatg ctcgccgtcc accatcacga taaagctcgc ttcttcgcca tcgaggaact
    32641 cttcgataac gatgcgatga cccgcgtcgc caaaagcgtt gcccgccagc atatcgtgaa
    32701 cagccgcttc cgcttcttcc agcgtcatcg ccacgataac gcctttcccg gcagccagac
    32761 cgtccgcttt aatgacgatt ggcgcgcctt tctcacgcag atacaccagc gcaggttcta
    32821 cctcggtgaa gttctggtat tccgccgtag ggatcttatg gcgggccagg aaatctttgg
    32881 taaacgcttt tgagccttcc agttgggccg cacctgcggt tgggccgaag attttcagcc
    32941 cggcggcgcg gaaggtatcg accacgcctt tcaccagcgg cgcttccggg ccgacgatgg
    33001 tcagatcaat cttttcgttt tgtgcgaaat ccaacagcgc cgggatatcg gtcacgccga
    33061 tagcaacgtt ttgcagcgcg ggttccagcg cagtgcctgc attacccgga gcaacaaaaa
    33121 cagtctcaac cagcggcgac tgggccgctt tccaggccag cgcgtgctcg cgcccgccgt
    33181 taccaatcac taatactttc atctattgct ccattaatgg cggaagtggc gcatgtcggt
    33241 gaagagcatc gcaataccgt gctcgtcggc ggcggcaatc acttcgtcat cacggatgga
    33301 accgccaggc tggattacgc aggtcacgcc cgcagcggcg gcggcatcaa taccgtcgcg
    33361 gaacgggaag aacgcgtcag aagccatcga ggaacctttc acttccaggc cttcatcggc
    33421 agctttaata ccggcgattt tcgcggagta cacgcggctc atctggcccg cgccaatacc
    33481 gatggtcata ttgtttttgg catagacgat agcattggat ttcacgaatt tcgccacttt
    33541 ccagcagaac agcgcatcac gcagttcctg ttcggtcggc tgacgttggg tgacgacgcg
    33601 cagttcttcc gcaccgacca tccccaggtc acgatcctga accagcagac cgccgttcac
    33661 gcgtttgaaa tcaagacccg gaacacgctc gccccactga ccgcaggtca gaacgcgtac
    33721 gttctgcttg gcggcggtga ttttcagggc ttcttcgctg gcggaaggcg caataatcac
    33781 ttcgacaaac tgacgagaaa tgatggcctg tgcggtttcc gcatccagct cgcggttaaa
    33841 ggcaatgatg ccgccgaatg cggaggttgg gtcggttttg tacgcgcgat cgtaagcatc
    33901 aagaatggaa ttgccgatag ctacaccgca agggttggcg tgcttcacaa tcacacatgc
    33961 cggctcggcg aactctttca cgcactccag cgccgcatcg gtatcggcga tgttgttata
    34021 agagagggct ttaccctgaa cctgggttgc ggtagcaacg gaggcttctt tcacattctc
    34081 ttctatatag aaggcagcct gctggtggct gttctcgccg taacgcatat cctgcttctt
    34141 aatgaagttc aggttcagcg tgcgtgggaa gcgaccggcg gcttctttgc tttcaccgtg
    34201 gtacgccgga accatgctgc cgaagtagtt ggcaatcatg ctgtcgtagg cggcagtgtg
    34261 ttcgaaggct ttgatggcga ggtcgaaacg ggttgcaagc gtcagcgatc cttcgttgtc
    34321 atccatctct ttaataatgg cgtcgtagtc gctgctcttc accacgattg cgacatcttt
    34381 atggttctta gcggcggagc gcaccatggt cgggccgccg atatcgatgt tctcaaccgc
    34441 atcttccagc gagcagcctt cacgagcaac agtctgggcg aacggataca ggttaacaac
    34501 caccatatcg ataggctgga tctgatgttc ttccataatg gcatcgtcct ggccgcgacg
    34561 gcccagaatg ccaccatgta ctttcggatg cagggtcttc acgcgtccat ccatcatctc
    34621 cgggaaaccg gtgtaatcgg aaacttcggt taccggcaga cctttttctg ctaacagacg
    34681 ggcagtgccc cctgtagaca gcagctccac accgcgtgcg gaaagtgcct gggcgaattc
    34741 gacgataccg gctttgtcag aaacactgag cagagcgcgg cggactggac gacgttgttg
    34801 catggtaaat cccttgggtt tgactattac agagagcgtt agctgaattt ttcgcgaaaa
    34861 actcagctaa cgcccctaac ggggcatcct tatttttcgc ccgcattgta acgaaaacgt
    34921 ttgcgcaacg ctcgcgaatt tttctctttc aatggtgatc acaattttga cagtggttac
    34981 cgtgggaaaa atacagaaat tacattgatg attgtggata actctgtgcg taaaaaggta
    35041 taaagcgggg ttttgctggg gaatgcagca gtcagtcatt tttctgcaat ttttctattg
    35101 cggcctgcgg agaactccct ataatgcgcc ttcatcgaca cggcggatgt gaatcacttc
    35161 acacaaacag ccggttcggt tgaagagaaa aatcctgaaa ttcagggttg actctgaaag
    35221 aggaaagcgt aatatacgcc acctcgcgac agtgagctga
//
LOCUS       DAJTYF010000029        33545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTYF010000029 DAJTYF010000000
VERSION     DAJTYF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.029"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            535..672
                     /locus_tag="OO590_001545"
     CDS             535..672
                     /locus_tag="OO590_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636951.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1272..2051
                     /gene="ipaJ"
                     /locus_tag="OO590_001546"
     CDS             1272..2051
                     /gene="ipaJ"
                     /locus_tag="OO590_001546"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6636952.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2999..3928)
                     /gene="virB"
                     /locus_tag="OO590_001547"
     CDS             complement(2999..3928)
                     /gene="virB"
                     /locus_tag="OO590_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6636953.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4145..4381)
                     /locus_tag="OO590_001548"
     CDS             complement(4145..4381)
                     /locus_tag="OO590_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6636954.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="OO590_001549"
     CDS             complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="OO590_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6636955.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTSPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="OO590_001550"
     CDS             complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="OO590_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6636956.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="OO590_001551"
     CDS             complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="OO590_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6636957.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="OO590_001552"
     CDS             complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="OO590_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6636958.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINKTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="OO590_001553"
     CDS             complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="OO590_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6636959.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="OO590_001554"
     CDS             complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="OO590_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6636960.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="OO590_001555"
     CDS             complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="OO590_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6636961.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="OO590_001556"
     CDS             complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="OO590_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6636962.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13569..15185
                     /locus_tag="OO590_001557"
     CDS             13569..15185
                     /locus_tag="OO590_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6636963.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLPQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15195..15557
                     /gene="ipgE"
                     /locus_tag="OO590_001558"
     CDS             15195..15557
                     /gene="ipgE"
                     /locus_tag="OO590_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6636964.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15557..16015
                     /gene="ipgF"
                     /locus_tag="OO590_001559"
     CDS             15557..16015
                     /gene="ipgF"
                     /locus_tag="OO590_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6636965.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16024..17139
                     /gene="mxiG"
                     /locus_tag="OO590_001560"
     CDS             16024..17139
                     /gene="mxiG"
                     /locus_tag="OO590_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6636966.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17147..17398
                     /gene="mxiH"
                     /locus_tag="OO590_001561"
     CDS             17147..17398
                     /gene="mxiH"
                     /locus_tag="OO590_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6636967.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17411..17704
                     /gene="mxiI"
                     /locus_tag="OO590_001562"
     CDS             17411..17704
                     /gene="mxiI"
                     /locus_tag="OO590_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6636968.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17710..18435
                     /gene="mxiJ"
                     /locus_tag="OO590_001563"
     CDS             17710..18435
                     /gene="mxiJ"
                     /locus_tag="OO590_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6636969.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18432..18959
                     /locus_tag="OO590_001564"
     CDS             18432..18959
                     /locus_tag="OO590_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6636970.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18901..19596
                     /gene="mxiN"
                     /locus_tag="OO590_001565"
     CDS             18901..19596
                     /gene="mxiN"
                     /locus_tag="OO590_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6636971.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKR
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19589..19996
                     /gene="mxiM"
                     /locus_tag="OO590_001566"
     CDS             19589..19996
                     /gene="mxiM"
                     /locus_tag="OO590_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6636972.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19980..20408
                     /gene="mxiM"
                     /locus_tag="OO590_001567"
     CDS             19980..20408
                     /gene="mxiM"
                     /locus_tag="OO590_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6636973.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20538..21170
                     /gene="mxiE"
                     /locus_tag="OO590_001568"
     CDS             20538..21170
                     /gene="mxiE"
                     /locus_tag="OO590_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6636974.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21157..22857
                     /gene="mxiD"
                     /locus_tag="OO590_001569"
     CDS             21157..22857
                     /gene="mxiD"
                     /locus_tag="OO590_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6636975.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSRLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22876..23943
                     /gene="mxiC"
                     /locus_tag="OO590_001570"
     CDS             22876..23943
                     /gene="mxiC"
                     /locus_tag="OO590_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6636976.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23956..26016
                     /gene="mxiA"
                     /locus_tag="OO590_001571"
                     /gene_synonym="virH"
     CDS             23956..26016
                     /gene="mxiA"
                     /locus_tag="OO590_001571"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6636977.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26029..26430
                     /gene="spa15"
                     /locus_tag="OO590_001572"
                     /gene_synonym="spaK"
     CDS             26029..26430
                     /gene="spa15"
                     /locus_tag="OO590_001572"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6636978.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26434..27726
                     /gene="spaL"
                     /locus_tag="OO590_001573"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26434..27726
                     /gene="spaL"
                     /locus_tag="OO590_001573"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6636979.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27819..28175
                     /gene="spa13"
                     /locus_tag="OO590_001574"
                     /gene_synonym="spaM"
     CDS             27819..28175
                     /gene="spa13"
                     /locus_tag="OO590_001574"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6636980.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            28144..29022
                     /gene="spa32"
                     /locus_tag="OO590_001575"
     CDS             28144..29022
                     /gene="spa32"
                     /locus_tag="OO590_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6636981.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIAANYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29016..29897
                     /gene="spa33"
                     /locus_tag="OO590_001576"
                     /gene_synonym="spaO"
     CDS             29016..29897
                     /gene="spa33"
                     /locus_tag="OO590_001576"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6636982.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29902..30552
                     /gene="spa24"
                     /locus_tag="OO590_001577"
                     /gene_synonym="spaP"
     CDS             29902..30552
                     /gene="spa24"
                     /locus_tag="OO590_001577"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6636983.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30567..30827
                     /gene="spaQ"
                     /locus_tag="OO590_001578"
     CDS             30567..30827
                     /gene="spaQ"
                     /locus_tag="OO590_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6636984.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30827..31597
                     /gene="spa29"
                     /locus_tag="OO590_001579"
                     /gene_synonym="spaR"
     CDS             30827..31597
                     /gene="spa29"
                     /locus_tag="OO590_001579"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6636985.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31604..32632
                     /gene="spa40"
                     /locus_tag="OO590_001580"
                     /gene_synonym="spaS"
     CDS             31604..32632
                     /gene="spa40"
                     /locus_tag="OO590_001580"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6636986.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSVFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32645..32887
                     /locus_tag="OO590_001581"
     CDS             32645..32887
                     /locus_tag="OO590_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6636987.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10472 a         4883 c         6710 g        11480 t
ORIGIN      
        1 tgacacttgt tgactacctg tgcggcatct ggtatcaagg tggcgtgaag ccggttcagg
       61 ttctcatgcg gaacgttgga acctatatcc ccccgatacc aaaggcgaaa tgtaagtgac
      121 aaaatgcaat gaacacgttc tccgttaact gaggagcatg gtgataaata aaggtaaatg
      181 cagaaggtgt agagtctcgc agatttctga catcatagag cctaacatta gatgcataac
      241 gctacagatt gtagaatctc tacaggtgaa atatacactg gttggcgttt atgtcgcaaa
      301 acctgtcatt caggttcatc tcaccaataa ggatatgagt gaagtggagg ataagtgagt
      361 ctgctgtcag agttttctgg tgtatgtcag taactgcgaa ccatgtctgc ttgatatggg
      421 agcttatgga ggctctctgt acaaggttag ggagctaaca aagcagggat aaaaagtccg
      481 cctgatgcag gcacggttcg ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat
      541 gttcaggcta tctttatctt gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca
      601 atagaagaac tgcaggcagt actggtccca catcgtttgc tcagtcaact ggtgaagttc
      661 cgtagcgcgt aaattaatgc tttgcatgaa acgttactgg agtggatgaa agaatacctg
      721 catatatcat tactgcgttg gattaccagt acaacagatt gaatgaactg gacatgttga
      781 tagacggcat tgaaaagcag cttataagca tagtgcggca gagttaattg gagagcactt
      841 cgccttctga gtattaagta agcatggtgg tacctgcgga tactatctat tcactgtgct
      901 cggtcggcag ccttactgac caaggagttg ggggagttga taacaaagtt gaaaaaacag
      961 ctctcaacca aggtggttat tgtaacaata gcaaataagc ttgtgtgacc tgtatcacaa
     1021 taaccgatta tggacgtaaa tacgacaaaa atatgtcagt atcaggcggt attaattgct
     1081 gatgctgatg gacattaact cttaataaaa gggtggatgc tgaaaggttg ttatggtgac
     1141 ggacgtgatg acgacggaaa agagtatgag gttttggatt taaagttcgt tcggggaata
     1201 aggacccggt cagctaattc tcatagtagt tcgcattgct ggatgcaata ctgtgctgca
     1261 attactcgtc gatgtcggaa caacggaagc cttgcaaacg gggatgtatt catacaggtg
     1321 ttatgttata tggggtttta ttgcaggggg ctataccaag agaatatatg atatctcatc
     1381 agactgatgt gagggtaaat gagaatagag taaacgaaca aggttgtttt ttggcgagga
     1441 agcagatgta tgacaattct tgtggagctg ccagtttact ctgtgctgca aaggaattag
     1501 gggttgataa aataccacaa tacaaagggt ctatgtccga aatgacaagg aagagttctc
     1561 ttgatttgga taatcgttgt gagcgggatt tatacttaat aactagtggt aattataatc
     1621 cgagaataca caaagataat atcgcggatg ccggttattc tatgcctgat aagatagtca
     1681 tggctacgag gcttcttgga ttgaatgctt atgtcgttga agaaagtaac attttttcgc
     1741 aagtaattag ttttatttat cccgatgcaa gggacttatt aattggcatg ggatgtaata
     1801 tagtacatca acgagatgtg ttatcttcta accaaagagt tttggaagct gttgctgtat
     1861 cctttattgg agttcctgtc ggcctccact gggtgttgtg tagacctgat ggaagctata
     1921 tggatccagc tgtaggagaa aattattctt gtttttcaac tatggaatta ggtgcaagaa
     1981 gaagtaattc taactttata ggatatacta aaataggtat ttccatagtt ataactaatg
     2041 aggctttgta aattgtcgtt ttttatctgt gaaggagctt gaatattttg gggcgggggg
     2101 ggcataacaa atgatattac tgagtacaaa tttagctata ttgggcggat tagttgtgtg
     2161 ggaagacgaa atgtttctct tgaaataagt gaactccctc aaggaatgat tgtgtgctta
     2221 aatatttaag tgctggtaag tttgagaagg ctgacatatt ttgcagagca aacagtgatt
     2281 gacttggatg atatttttat tttagtcact cgttgtaatt ggagtatgct ttattttatt
     2341 tgaaaataga tgtattttat acccgcaatt taaactgtaa gccctatgag gggtatggat
     2401 attataagtt actctttgac taagcataac ttttggggtt agaaaaataa atctcttgtt
     2461 aataattaat atgattttaa aatcaaactt gtttataatt ataaaataat gtaagtcggt
     2521 cattttatgg gaagattagg agaataaaac agtaattata tttgagaaat ctgctaatat
     2581 attcttaaag cttttttgat actgtgaacg aagtctgtta atggtggatt ttttgtttat
     2641 caccatgtaa aaaaattgag tagtaaaagc gttgactgct aatcctcgct gtttttattg
     2701 aaacgattaa aaatacgctg aatattttcg gccagtcact catcttcaca taaaattttg
     2761 accatcatgc cgcatccctt agttgcacaa ttcattttca atgatatgaa tattactaat
     2821 ttcaccagat aattttctcg tggtcagcag ggtatgagtt ttcaccgtaa ttttaaggaa
     2881 acacagtgcg aggggattga ataacagata gccgacggaa ttaccccacc ggctgaaagt
     2941 caagttcaac gaagattttt attatctgaa ttgggcagtt tacatcagtg ttcgatgttt
     3001 atgaagacga tagatggcga gaaattatat cccgaatagc ttcatctatt tctctctgaa
     3061 ctgttttgtt tattttagat aatgtaaata ttagagttct gtttgtttta ttctcttttc
     3121 tttttatgta agtccgtttg tctttagaaa taaataacga gtcaactttt agcgaagggt
     3181 tttgctttct gtttttgctt ttctttatga tgtttaaaat ttctttttta tatatgtccg
     3241 ggggcaaatt tgtatcaagg tcttttattt cttcctttaa tattggtagt gtagaactaa
     3301 gagattcatt agccttttca aggcctttat aataattgaa taatatcttg tagtcattaa
     3361 agttaagttc tgacgcgatt ggaaatagag atattatttc ctgtggaacg cttgctgcct
     3421 gaaaggcacg agtgactttc gcgcgagaca gattctcttt tttggctatg tctttatagg
     3481 acatccctga tactttcaga aaattaagac caataccaag ttctcggatg ctatgctctt
     3541 ttgctgtttg tatatcgttt gctagttttc tggcatcaag agtagatata tattcttttg
     3601 aatatagaac ttcaagatct gctcctgcat atattgcaga tgctcttcta cgagtgccat
     3661 ccagaatttc aattctacca tcaatctccc ttcctattac agggaagaat tgttgtagct
     3721 ttatagtttt tatgatatcg gctaatgatt cttctgttag ggattcttga tcgcgtacat
     3781 tcgttttttg gttgacgaag gttaaatctt ggatcctttt atgaggaatc ttggctttga
     3841 taaaggaaac tttattacca gaatggagtg taaactcttt cttttggcct tcctttatac
     3901 ttaacaagtc gttgcacaaa tccaccatat cacaccctgt ttattcatat tgatttatat
     3961 tgacaaaagt taaatgcagt gtgagcaaga ttgtatgtaa ctgttgatga tagtcaacgg
     4021 tagtcttaaa aattactatt ttaatagctg gattgatgca atttatcttt attggataaa
     4081 agaataatat tcttgtggag ctctgatgtg agagctccat agatgaaaaa atgcttatca
     4141 attactatat tgattttgta ctatttttga ctacagaaat aagatctcca atagtaataa
     4201 tgtgttctaa tgcagattca tctatacgta gattgaactc aaaactaatc atatccagca
     4261 tttcaatcag agaaagagaa tcggcagaaa gatcttcaat aaatttagtt tcttcagata
     4321 gtttgctgta cgctatacca tagcaaaagg ccactattga taatattttt tcttttatca
     4381 tattttaatc cttattgata ttctttaata cttttgatag ggaagtggtt acatcttttg
     4441 ctgctttata tatattattg ttttcagctg taatatcatt gttatttttt aaatcagata
     4501 tatcatctgt aagtaattct gcagaggtat cgtcaatttg tgacaatacc ttgctgaggg
     4561 cgctagatac ttcttttgcc ttttcataga tcgcatgatt tttatcaatt gtatctgcgt
     4621 tggtttctgt tacgtagttt attttatttg catctgtaac ttttaatttg ttgtttattc
     4681 tgtgttctgt tgtggatttt gtaatgtcct ttgatttttc ctgtaactct cgttcttgtg
     4741 ttcctgattt agttccaatg agagatataa gattacttag ggagtttgtt actttttttg
     4801 aagcttcaaa tatcgtttcc cgtgttaaat atgtgtctcc atttcttgat aaaaaatcag
     4861 tctgaagtac ttttgaatca gtgtttgagt tgaatttatt tatttgcgaa gctactctat
     4921 gagaaagaga agcgctactt ttagttgtat ctgatgagtt atcattactg cttgtcactc
     4981 cttccgggtg gagaggggag gtggttgaag agttctgtat gttcagaggc ctgtgtcccc
     5041 gagaaagaga gttatttgca gatctttctt gactactcgt agttaacagg tctgaaccta
     5101 ttccatccga attatctgga gtatggttga aaaccttttc taaagctgaa ttatttgcaa
     5161 atgtctggac agtatctgat atagagtgtg gtagaacgga agttactttt tgaactagtg
     5221 ctctttgtgg atttagtaat gagtttccgt tacgggataa tagatcgttt gtctgagaag
     5281 taggagactg tgcatcattt tctgggttct cgtgatagct attgtcatat actctgttgt
     5341 caaaaactct attatcgtaa gttctattat cattatttat atgatagtga attacaggtt
     5401 gggatgtttt ctcattggct ccgggggttg gtccgccatc tggcatatct tcgggaggtg
     5461 tagggaggcc atcttggata ggatgcgaat cttttatatc ttctaaaata tcattaagga
     5521 cttttctcaa cagctcagga aacacggcta ctgtatttaa gtctcttgac agtttaccta
     5581 taccaaatcc tttactatca atagtatttt tacgttgatt gcgcaaacta tcaaaggcaa
     5641 gttttgcctg tgcgtcaatt aaatcatcta gtttgagaga tgaaacagtt tcattactta
     5701 actttaattc agcaagaata ttattcttga taaatgatga tacttcagta ttgaggagtt
     5761 tttgtcccaa tgataaagct tcaccagatg tgtgttgttg tgctaatagt gcaagtctgt
     5821 gctcaatgtt ttgtcgtaat tgatggagtt tcttattctt tgccgaccat ggtccaaagg
     5881 cagaatcttg ggataattta tcttttataa ttagttccga atactgtgcg acaatttttt
     5941 gaagtccctt ttcgttttca aaattaagac cattttgatg tactaaggag tacaaaacat
     6001 ttgaaaattc gagaatctcg cttttggcat acgcattcat tgagttggaa taaatgcttc
     6061 ttaacacatc ttctttccct gaggaagaaa ataaaaaatc aagacatttc tgattaaaag
     6121 acgttgcaaa gttttctttt tcagacactg atgctggtgt ttttagagaa gtttgcgaac
     6181 tatggatatc ggatattttg ctttttaact cgctgtattc tgttgatgat ggtgaagttg
     6241 ccttatataa gaatgttggc gcttgagtat tatttacatt atgcatggcg cacctcagaa
     6301 atggagaaaa agtttatctg tatctgtaca tgagcttatt gtactactca aaacctttac
     6361 taaattatca aaaatactat tggcattact gtatttttga actaaagttt gaagattatt
     6421 tttcattgtt tcatcttcgg cagagaatcc ggcattccat gcctgatatt ttgcattatc
     6481 tagcacaacc tcgccatttc cacctagatt atctaagctt tttaacatat tgtctattgg
     6541 ggtcatgttt atactgacaa catatccccc gtttttttga gataccttgc cgattgttcc
     6601 acctaattct gtaagccatt tatttgcttg ttcctgacta acagtattat ttgctggata
     6661 tagcggttta tctttatatt tttccttgag ttcttccaat gcctttttaa gcgagttgac
     6721 ttgtaatttc acggagtttc cgtcgttacc tccgggagag atccagccgg caagactgga
     6781 aagaacagcg ctaaaatctt gatacatttg agtatatgaa ctaacggcat gttcatatac
     6841 tttcagatac tgttcattaa tatcattgat ggagtttgca attttagccc acagttctct
     6901 atgagatatc atttgatctc catcaagctc ggcttctttc ggggctgaat gtaataattc
     6961 tcttgcgtct ttattaattg gatattcgtt cctggaaaga atatccaata gttgagcgga
     7021 tttatttaca tccatgctaa tctgagatga atgtaatgct atttcttcta gcgaagtttt
     7081 agtcaacgtt ttttgtgaaa gctctttctt taatgcctta tttgttgttc tgatattatg
     7141 aagggtgtcg ttaagcatag taagggaact tacaggatga gaactggtcg ttgtttttat
     7201 atcagaatta actgtttcgg ttgatgaacc gttggtattg tttggactga atgatgaggt
     7261 ggaaatacta ttagtcagag ttgttatatt catggttatt tccttacagg aagagccata
     7321 tattattatg gctcttggat atattaagct cgaatgttac cagcaatctg actggctgcc
     7381 gaattccttg attgggtgat gctgtcaatt atattcaata atttttgtat taattgattt
     7441 gtcgcttggg atgcttcttt agatacttgg gatgcttcct ctgcttgttt actgctggcc
     7501 tgactgatta gttgttcttc ttcttcaaga gcagatgcat aacgccctcc tgatgtggat
     7561 atattaccag caacggctga tgtttccatt gttgcacggc caatgttttg tttttgctgc
     7621 gcagaaaggg tattgagctc gtaagttctt ctctgggtgt caattttatc ctgcaaagaa
     7681 atatcgggag aactaagaga agttttatgt tcagttgaca gggatatatt atctggcgcc
     7741 agtttatttt tacctaaaaa ttttgtgcta gagttagttt gtggtgaggt gatatttgta
     7801 tctatttgtt tatttaaccc taatttagaa cctgcaagct ctttttcaag agattgagca
     7861 gtggcaaggt tctttcttaa ggctcctttt tggtcgctaa tccctgaatg cgttttttta
     7921 gcaccgatac ccgttatacc tacttgtgtg actgctccag taatgcttga tgatagggct
     7981 gccaggcctt gccgaacaat gttctctgca gctgattttg tagcatcgaa cgcaatcaat
     8041 gacaattgag agcccaattt attttctgca gtgcggagag cagaaagtag aacggctaca
     8101 ctaataatta aagaaacagc attagaagaa agactggaaa tatccagagt gttctctgga
     8161 gtaagggtgt gactcatctc tgaaagatct ttcatctttt tgtcagtaag gcgagcaatg
     8221 atttctgaat catgctgaac ctgctctgat aactttaaaa gttgatcatc atttaatgtg
     8281 gttgttaata cgggattttt accgacatta agtggaatat gcgctgcaat ctgctgataa
     8341 ttttgtgatt tttgtgtttc ggaagaactt tgagtttgtt ttgtggatat atctgtatat
     8401 aaaatctggg ttggttttgt gttttgaatt tccataacat tctccttatt tgtatcaagc
     8461 agtagtttgt tgcaaaattg cttttgcaac atcagttcta ttagcctgag agttggacat
     8521 tgaggctaat agatctgcaa ttacttcctg caattggccg aatttttcta ttgcttcact
     8581 gatatatttt gacagttgtt caacttgata tttcgataat gtcaggtctg ctagatttgt
     8641 cgacgcgctg ttctggaaaa cagcagaagc gacacttcct cccgcttgtg tggcagagtt
     8701 aacactttct cctaataaaa ctgcttggtt taaatgggtg gaaataattt gtttggatat
     8761 tacttcatca cccgctgcac caagaaattt atttaatctg gcaacagcat tagtgattaa
     8821 atcgtccagt tgagaagaaa aattcttgag aaactttggt ataaggtctg tgagggtttt
     8881 acctattatt ttgccaatat tctctgcaag ttttgctgct gcctgtttac caacagtggc
     8941 tacgagaacg actgctgcaa ctaggacaag agcgcctgcg attgccccca gaatagagcc
     9001 aatcattttg gcttttttcg agtcgacgcc caagccttcg agcatttttg taaatgcatc
     9061 tgaaaggagt ttgattaagg gttcaatgac tgctttcatg atcggattca gggcttgttc
     9121 catgaaggaa ttgccggtcg ctgcttgtac tatagcatcc gtaaccataa gagctaaacc
     9181 aacagctgcc agtgctagag aggctcctcc agaaaaagct gctgcaacaa cactaacgat
     9241 agttaaaagt gccccaagta ttttcccaac acaacccatt actctgttga gttcttctgc
     9301 tttacgtact tcagcagcat actcatcaga ttttctctcc atttcagttt ttcttgattc
     9361 ttggagagac tggaatagag ccagatcatt ttttaaagat tcttcattat tttttccaac
     9421 tagttgaata aaggttgcca tcaattgagt aacactggca agtccggtta atgatttctg
     9481 ctgggttgat agctgttcag cagatgctgt gtttgaaaat gcagaaaaag agtctatttc
     9541 tttttcgagt tgcattgatt tatctgtaag tttgctatga attgacaggg ttttctgctc
     9601 aatcaatgtc ctgtctttaa ctgctgcgtc ttttttgata gtgagttgta tttcttcccg
     9661 gcttaatttt ttcttttctg gtgactctgg gtcgagttcg gataatcttg tttgaatttg
     9721 gttaatttta ttttctaggt cttttatttt agaatctgcg ttttttagtt tattaatttg
     9781 tttttcatag tctctggtta gtccttcagt ttcagataga agagtgttaa ttttatcgga
     9841 gaattctagg tttttttgct gtcttgcctg ttgctgggac ttccaagcag taattttatt
     9901 tgttaatgca gttaaagatt tttcaccgag tatttgaata aggtttccaa ttaaaagcgt
     9961 tagttgggaa cttgcattta atgactttgg tgctttaagt tcagggatta atatatttga
    10021 agttgagtgt gcattagttt tattaatatt ttggttagca gtaagatcag caattgtaag
    10081 agaaaataat ttattagctg catcatttgc agcttggata gtattgtctc caagctcagt
    10141 ggaagccaat attttggcaa gaggaaaacc agtggttgtg gtgcttacat tatgcataat
    10201 aattactcct tgatatcctg aattgcgtcc aagtatgatt gtgcttttat ttttaatttt
    10261 tcatcattgc tgtgttgaat tacgagttcg aagcactctt tagcttttaa gggggctttc
    10321 aaccgaagct ggcattgtcc agtatggaat actggtgtat agtcattttt tcctaatgca
    10381 aaagcgacag cataaaggtc tgctgcttgt tggaactgtt cttttatctg ataaatagct
    10441 gcgagtccca taatgtagtc tacattgtaa aagtcgtata tacataaaaa tctgaagaaa
    10501 acttcagctt cctctattct tcctttgttg taaaagtcat aagcatatga ataaatgtca
    10561 tccatcatat catcaggaat tgcattaata tctttcagtg tagcgccaga gttaattgca
    10621 tcaattactg cagtagagat gctttcattt tcagtgatat ttaaagacat aaggtctcct
    10681 ttttgtctgc tttactttaa taagtataag atttaatata aaagatttaa tttgtattgc
    10741 tttgacggta tacagccttt agaacctctg cgcttatttc tatttgtaaa acttttgttg
    10801 attcattaaa tattttatcg tagacggaac tggatactag atttgtaaga tccttcattt
    10861 gtgtgttaaa caaatccatg tcactgctat agcgttttaa aatctttgct ctgaggccag
    10921 atgaggcaat ctcgtttagt gctgtatgac tgatagatgt ttgtgctgat ttttcatcaa
    10981 gttttatatc cgggaatttt ttttcaatag ctgcaaatat gttttttttg ttatctgagg
    11041 tcaaaacaac tccattgtgt atggccattt catctatact tctgtttatc attgaagaga
    11101 cataagaggt tcgctcttgg ctcatccaac aaaatagttg attccccgaa ttactatctt
    11161 ttattttgtc tatgacgctg tccctgattt tatttatatt caccgggcat ccaaaattct
    11221 tctgaggaaa gcgttgtttt aagttaaaaa cagataatat tttctttaca agcttattgc
    11281 gacttaacga atcaaatgat gggcgaggaa tcgcgaattc aacctgtgga agtattttgt
    11341 ttagaatttg catgataccc cctatatgtt agttcacttc tgaagtgatg tttgcaatgg
    11401 ttagtttaat acaaaataaa aattcataca aagattcaat gagttcatca ggtgaaagat
    11461 attttttgta gtgtttcttg gtcaaaacac attgatgagt atcagggaga agacagatag
    11521 aagaaccata atctctactc aaagagtagt taaggatgag tatgtgttct ggattaataa
    11581 tattttctgt aaaaagttca tgttcattaa gaagtccggt caaaaagatg tattcatcat
    11641 tatctgttat acaaacaggt atttgttctt cgagcaatat aaaggcttga tttttatcgt
    11701 taaaatcaag ctttgcacct gttatttcat aaagtttatc atatagtttg cgacacattt
    11761 ctatggcctt ctatatatta gaatgagagt tattcaataa aagcttattg atctgtaata
    11821 agtcgtgaat gtaaagtttg gttccaagat ctggcgattt aagagaattg taataatcaa
    11881 taatttttat tgatttttcg ataatttctt cttttttatc tgttgcttcg gtttttaatt
    11941 cgtttaatac acgagtcata ttgctgggtg ctgattttac taagtatgag tggcaagcgt
    12001 tgatgaccac atcaaagtct tctgaagata cattttcagt aggatgtttg taaagtgaaa
    12061 ttgataaagg cttgaatgtt tttggtgtgt ggataaggtt aattttattg aaacttttaa
    12121 gatagctgcg tcgtaattga gtcaatctgg aaagatcaaa atcagattca atatacttca
    12181 ttatttcgtt ataagactga ttgagacttt caatgcgttg cctaacttta tttgcatacg
    12241 cgtatacatc gttaggtgta gcaaccaatt taacgtcagg gagtggaaag tatatttcag
    12301 aatttcctgc tttaagaaca ttaagtgcca taccagcaca gttttcagat ttactgatta
    12361 atttataatt tgccaattgt ctgaaagcat ctgctttctt tttgcatata aattttgaca
    12421 tgtttgtttc atcaaggcca aaaaggttat ggctgatttt accatccttt gttttattat
    12481 cacctgataa tggaatgtat actttatcag cactgacccc ccaataacca taggggtctt
    12541 ttttttgatt agctctggga aaaaatattt ttttgttgtt attttgatct tgttttgaat
    12601 ttaataagtt tctaacctct tggcctgcat caagtctttc ggctgtttgg tctgaaatca
    12661 tatttagttt gtcttgctta taggtatcga cactgtaacc taagcgtttt tcaaaaaggt
    12721 agtttatttc tgtatctttt gtaacatccc cgccgggata ccagctgaga taggtatgag
    12781 ttggttcctg tagtaaattg ttttttatac tgatactagt atgccctaaa tgggttatgc
    12841 ctctatttcc ccagcaacga aaaactaaat gtctttcaag atttttggct gtaccgatgc
    12901 catgaaaacg ctctgcggta ttcaacatta atttgatgtc tctacctgtg aaagagtgtt
    12961 gaattgagtt tttagctgct tcataggcgg caaacgcact ttctgcagat ttttcgctag
    13021 ctaatgtatt gaggaaaagc aataaggatt tttgattctc tagtcgataa ttttttagtt
    13081 gttcatttaa tatctgtgaa tttatctttg cattaatagt gcttaatgtt ctgaaaaaga
    13141 gatcctttag gttgaatttc tgtgctatca atataggtcc atgctctgta tcttcaacgc
    13201 gtataggccc ttttgtattg cttgcgtcaa tgaaattgct aattttgagg ctcatacttt
    13261 attaactctc cattacttgg tgattgtggg gatgtaaaat gtatttggca tataatatat
    13321 tcaattaata aatttagaaa cttgagcctg ttaacataat caaattttct tttgctgtac
    13381 ataatatgta cctcgtgagc atatgtagtg ctcgtttcat catgaaatcc cacaagataa
    13441 agtgcctgat gtatcaggct cggagtgtta tagaaaaaga gagaaccctg ttgaataagt
    13501 atcttttttg ttgtaaaacg aggtacaaaa ctgcaatcaa tgccccattt atgactgagg
    13561 ataattaaat gcacataact aatttgggat tgcatcaggt ttcatttcaa agcggagatt
    13621 cctataaagg cgcagaagaa accggaaagc acaaaggtgt aagcgtgatt tcatatcaaa
    13681 gagttaaaaa tggagaaaga aataagggga tcgaagcgct taatcgatta tatttacaga
    13741 accaaacaag cttaacagga aaaagccttt tgtttgcgcg tgacagagct gaagtttttt
    13801 acgaggcaat aaagttggca ggtggtgata cgtcaaaaat taaagccatg atggaacgat
    13861 tagataccta taaacttggt gaagttaata aaagacatat taatgagctt aataaagtaa
    13921 taagtgaaga gataagagca cagctaggca ttaaaaataa aaaggaattg caaacgaaaa
    13981 taaaacaaat atttactgat tatttaaata ataagaattg gggaccggtc gacaagaata
    14041 ttagtcatca tgggaaaaat tatggttttc aattaacacc tgcctctcat atgaagatag
    14101 ggaataaaaa tatatttgtc aaagagtata atggaaaagg aatttgttgt gcttctacga
    14161 gagagagtga gcatatcgcg aatatgtggc tttcaaaagt ggtggatgac gaggggaagg
    14221 aaattttttc gggtatcaga catggagtta tatcagccta tggattgaaa aaaaactcat
    14281 ctgaaagagc tgttgctgct cgtaataaag ctgaggagtt agtaagcgca gcattatata
    14341 gcagaccaga gttattatca caggctttgt ctggtaaaac agtagattta aagattgttt
    14401 cgacttctct tctgacgccg accagtttaa ccgggggaga ggagagtatg ttaaaagatc
    14461 aggtgaacgc attaaaaggg ttaaactcta aaagggggaa gccaacgaaa ttattaattc
    14521 ggaatagtga tggtcttctg aaagaagtaa gcgttaatct aaaagtggtg acatttaact
    14581 ttggtgtaaa tgaattagcg ctaaaaatgg gcttaggctg gaggaatgtt gacaagctta
    14641 atgatgaatc aatatgttct ttgttggggg ataatttcct caaaaatggt gtgattggag
    14701 gttgggctgc agaagcaata gaaaaaaatc caccatgtaa gaatgatgtg atatatctgg
    14761 ctaaccagat aaaagagatt ataaacaaga agttacaaaa aaatgataat ggagagccgt
    14821 ataaattgcc acaaagaatg actctattgg cttatactat aggtgctgta ccttgctgga
    14881 attgtaagag tgggaaggac agaacaggta tgcaggatgc tgaaattaaa agagaaataa
    14941 taagaaaaca tgaaacaggt cagttttctc aattaaatag taaattatcc tcagaagaga
    15001 aaagattatt ttctactatt ctaatgaata gtggtaatat ggaaatccaa gagatgaata
    15061 ctggtgttcc cggaaataaa gtcatgaaaa aattgccgct atcttcgtta gagctatctt
    15121 attctgaaag aataggggac ccaaaaatat ggaatatggt gaaagggtat tcgtcatttg
    15181 tataagagga atatatggaa gatttagcag atgttatttg ccgagccttg ggtatccctt
    15241 taattgatat cgatgatcaa gcaatcatgc ttgatgatga tgtgctaata tatattgaaa
    15301 aagaaggaga ttcaataaat ttattatgcc ctttttgtgc attgcctgaa aatataaatg
    15361 atcttatata tgcgttgagc ctaaattact cagaaaagat atgcttagct actgatgatg
    15421 aaggcggaaa tctaattgcg cgattagatc ttaccgggat aaatgaattc gaggatgtat
    15481 atgttaatac agaatattat atttcgcgtg tgcgttggct gaaggatgaa tttgcgcgaa
    15541 gaatgaaggg gtattaatgt cccgtttcgt atttattttg ctgtgtttta tcccatattt
    15601 agggagagct gattgttggg ataaggctgg tgaaaggtac aatattccat caagtttgtt
    15661 aaaagcgatt gcggaaaaag aatccggatt taataaatct gcagtcaatg ttaataacaa
    15721 tggaagtaaa gattatggta taatgcaaat aaatgacttt cattctaaaa gacttagaga
    15781 aatgggatat tctgaggaaa tgctaattag tcatccatgt ctttctgtac attatgcagc
    15841 aaagttatta aatgagttta tgatgatgta tggaagaggg tgggaggctg ttggagccta
    15901 taatgcagga acgtctccga aaaagaaaaa agaaagatta aagtacgcag aggatatcta
    15961 tagacgctat ttgcggatag cagcagagag caagcagaat aatcgaagga tataagagga
    16021 tttatgtctg aggcaaagaa ctcaaatctt gcaccattca ggttattagt gaaactcaca
    16081 aacggtgtcg gggatgagtt tccgttatat tatgggaata acttaattgt tctcggacgg
    16141 acgatagaaa cacttgagtt tggaaacgac aatttcccag aaaatattat tcctgtaaca
    16201 gatagtaaat cggatggtat aatttatttg accataagca aagataatat ctgtcaattt
    16261 agtgatgaaa aaggtgagca aattgatatc aattcacaat ttaattcatt tgaatatgac
    16321 gggatttctt ttcaccttaa aaatatgagg gaggataaat cgagaggcca tatactaaac
    16381 ggaatgtata agaaccactc ggtgtttttt ttctttgctg ttattgttgt gttaattata
    16441 attttttccc tctctttaaa gaaagatgaa gtaaaagaaa ttgctgaaat tatagatgat
    16501 aagagatatg gaatagtcaa cactggacag tgcaattata ttcttgccga aacacaaaat
    16561 gatgcggtat gggcgtctgt agccttaaat aaaaccggat ttaccaaatg cagatatatt
    16621 ctggtgtcaa acaaagaaat aaatagaatt caacaatata tcaatcaacg attcccattt
    16681 atcaatttgt atgtgttgaa tttagtgagt gataaagcgg aattactggt gtttttaagt
    16741 aaagaaagaa atagttcaaa agataccgaa cttgataaat tgaaaaatgc tctgattgtt
    16801 gaatttccgt atatcaaaaa tattaaattc aactatctta gcgatcataa tgcaaggggg
    16861 gatgctaaag gtattttcac aaaggtaaat gtccagtata aagagatttg tgaaaataat
    16921 aaagttacat attctgtcag agaagaactg acagatgaaa agcttgagct cattaataga
    16981 ctgatttctg aacataaaaa aatatatgga gatcagtata ttgagttttc tgtacttttg
    17041 atagatgatg attttaaagg taaatcatat cttaacagca aagacagtta tgtaatgttg
    17101 aatgataaac actggttttt tttagataaa aataagtgag gataaaatga gtgttacagt
    17161 accgaatgat gattggacat tgagttcatt atctgaaact tttgatgatg gaactcaaac
    17221 attacaaggt gaactaacat tggcactaga taaattagct agaaatcctt cgaatccaca
    17281 gttgctggct gaataccaaa gtaaattatc tgaatataca ttatatagga acgcgcaatc
    17341 caatacagtg aaagtgatta aggatgttga tgctgcaatt attcaaaact tcagataata
    17401 gggagcattc atgaattaca tttatccagt caatcaggtt gatattatca aagccagtga
    17461 ttttcaatct caagagatat caagtctgga agacgtcgtg tcggctaaat atagtgatat
    17521 taagatggat acagatattc aagtatcaca aataatggag atggtaagca atccagaatc
    17581 attaaaccca gaatctttgg ccaagttaca gacgacgctc tcaaattatt caataggagt
    17641 atcattagct ggcacgttag caagaaaaac agtttcggct gttgaaactt tattaaagtc
    17701 ttaatttata tgattaggta taaaggtttt attttattct tgttgctgat gttgattgga
    17761 tgtgagcaac gtgaagagtt aatttctaat ttatctcaaa gacaggcaaa tgaaataata
    17821 tctgtgctag aacgccataa tattactgct agaaaagttg atggaggtaa acaggggatc
    17881 tcggtacaag tcgaaaaggg gacatttgca tcggcagttg atttgatgcg catgtacgat
    17941 ttgccaaatc cggagagagt tgatatctca caaatgtttc ctacagattc attagtgtct
    18001 tctccaagag ctgaaaaggc ccgtttatat agtgctattg agcaacggct ggaacagtct
    18061 ttagtttcta ttggtggtgt tatttcggca aaaatacatg ttagctatga tcttgaagaa
    18121 aaaaatatat cttcaaaacc gatgcatata tcagtaatcg ctatatatga ctcaccgaaa
    18181 gagtctgaac tattagttag taatattaag cgatttttga aaaacacctt ttctgatgtt
    18241 aagtatgaaa atatatctgt catattaact ccgaaagaag aatatgttta tacaaatgta
    18301 caacctgtta aggaagttaa atcggaattt ttaacaaatg aagtaatata tttatttctc
    18361 gggatggctg tactagttgt cattcttttg gtatgggcat tcaaaacagg gtggttcaag
    18421 agaaacaaaa tatgataaga atggatggaa tttataaaaa atatctttca ataatttttg
    18481 atccagcgtt ctatataaat agaaatcggt tgaatttgcc ttctgaactg ttagaaaatg
    18541 gcgtaatcag aagtgagatt aataatctca taattaataa atatgatcta aattgcgata
    18601 ttgaaccttt aagcggggta accgctatgt ttgttgccaa ctggaattta cttccagctg
    18661 ttgcttattt tatcggtagt caagagtctc ggttaattaa tcatagtgag atggttatta
    18721 gttattatgg gggcaaaatc agtaagcaag gggaggccgc tattcgaagt ggtttttggc
    18781 atcttattgc gtggaaggaa aatatatctg ttggtatata tgaaagaata aacttattat
    18841 ttaatccaat tgctttggag ggaaattata cgcctgtaga aagaaatttg tcccgattaa
    18901 atgaaggtat gcaatatgca aaaaggcact ttaccggtat ccagacatca tgcctatgat
    18961 ggtgttgtta taaagagaat tgaaaaggaa ttatgtaaaa ccataaaaga tagagacact
    19021 gagagtaaga ggaaagcaat ttgtgttata aaggaggcta cgaaaaaagc tgaatcactg
    19081 cgtatagatg ctgtatgtga tggttatcaa attggtatac agactgcttt tgagcatatt
    19141 attgactata tttgtgaatg gaagttaaag caaaatgaaa acagaagaaa tattgaggac
    19201 tatataacta gtttgctttc agaaaattta catgatgaga ggatcattag cactttattg
    19261 gagcagtggc ttagttcgtt gcgtaataca gtaacagaat taaaagtggt tttgcccaag
    19321 tgtaatttag cactgagtaa aaagttagaa cttgatctcc ataaatatcg ctcagatgtc
    19381 aagattattt taaaatattc tgaagggaat aattatatat tctgtagtgg taatcaagta
    19441 gtggagttta gtcctcagga tgtaatatca ggtgttaaaa tagaacttgc ggaaaaatta
    19501 acaaaaaatg ataaaaaata tttcaaagag ttagcacata agaaactgag acaaattgca
    19561 gaagacttgt taaaagagaa tcctgttaat gattaatcaa ataaatgcaa gcaatgctct
    19621 gcagcaaaga ttaaatagtg aagaatttgt taatttaaat gagagattat catcttcgca
    19681 atcatttgat gaggatataa tctatgaaat aatgcaatat ttctcacaga gtgaattaaa
    19741 ttccatagat aatgatgaac tgcacaataa gattgaacaa ttattcaatt cacgttttcc
    19801 ttatcttaca gctgcacaaa aaagttcact tcttaataaa ctcatagatg caaatcaata
    19861 tgtcgatttg catgagggat tttatgcatc attgtccata tataacaata ttgatttcta
    19921 tattaagact acaaccttcg attcattaat tagtgtcttt gaagcaggga gagaggcaga
    19981 tgattcgaca tggtagtaat aagttgaaaa tatttatttt aagtatattg ctattaacac
    20041 tgagtgggtg tgctttaaag tcatcatcta attctgaaaa agaatggcat attgttcctg
    20101 taagtaagga ttatttttct attccaaatg atttattatg gtcgtttaat acaaccaata
    20161 aaagtataaa tgtttactct aaatgtatta gtggtaaggc ggtttatagt tttaatgcag
    20221 gtaaattcat gggcaacttt aatgttaagg aagtagatgg gtgcttcatg gatgcacaaa
    20281 agatagctat agataaacta ttttctatgc tgaaagacgg ggttgtttta aaaggtaata
    20341 agataaatga taccatcctt atagagaagg atggggaagt taaattaaaa ttaattcgag
    20401 ggatataatt gtattgtgag taaatataaa ggtctaaata caagtaatat gttttacatt
    20461 tactctagtg gacatgaacc agttaacgtt gagcttgtaa aagataaaga acgtaacata
    20521 attgagctgg ctccagcatg gaagggtttt tttttgtccg aaatcaaaat ataaaattca
    20581 gtgataatgt gaactatcat tatcgcttta acattaatag ttgtgcaaaa tttcttgctt
    20641 tttgggacta tttcagtggt gctctagtag agcatagcca tgctgaaaaa tgtattcatt
    20701 tctatcatga gaatgatctt cgggatagtt gtaataccga atcgatgctt gataagttaa
    20761 tgttaagatt catcttttca tctgaccaga atgtaagtaa tgcactggct atgatacgaa
    20821 tgactgagtc atatcatttg gttctttatc ttctgagaac tattgagaaa gaaaaggagg
    20881 taagaataaa gagtctgaca gagcattatg gtgtgtcgga ggcttatttt agatctttat
    20941 gtagaaaagc tttaggggca aaagtcaaag aacaactgaa cacttggcgg ttagtaaatg
    21001 gtttattaga tgtatttttg cacaatcaaa caatcacttc tgctgccatg aataatgggt
    21061 atgcatcaac atcccatttt tcaaatgaga taaaaacacg actaggcttt tctgcaagag
    21121 aattatcgaa tattactttt ttagtgaaaa aaataaatga aaaaatttaa tattaaatct
    21181 ttgactctct tgattgtatt gttaccattg atagttaatg ctaataatat tgactctcgt
    21241 ttattagaac aaaatgacat agcgaaatat gttgctcaaa gtgatacggt aggatctttt
    21301 tttgaacgtt tttctgcgtt attaaattat cctattgttg ttagcaaaca agctgcaaaa
    21361 aagagaatat ctggggagtt tgatttatct aatccagaag aaatgcttga aaaactgaca
    21421 ttacttgtgg gactcatatg gtataaagat ggaaatgcat tatacatata tgactctgga
    21481 gaattaataa gcaaagttat tttgctggag aatatatcac ttaattatct tattcagtat
    21541 ctgaaggatg ctaaccttta tgatcacaga tatcctataa gaggaaacat tagtgataaa
    21601 acattctata tatctggtcc tccggcattg gttgaactgg tagctaatac agcaactttg
    21661 cttgataagc aggtttcttc tattgggaca gataaagtta attttggggt tataaaatta
    21721 aaaaatacat ttgttagtga cagaacttat aatatgagag gggaggatat tgtcatccca
    21781 ggggtggcta ctgttgtaga acgtttatta aataatggta aggctctgag taacagacaa
    21841 gcccaaaatg atccgatgcc accctttaat attacacaga aagtatctga ggatagtaat
    21901 gacttttcat ttagttcagt taccaatagt tctatattag aggatgtatc acttattgca
    21961 tatccagaaa ccaattctat attagtcaaa ggtaatgatc agcaaataca gattattcgc
    22021 gatattatca ctcagttaga tgtagccaaa cgacatatag aattatcatt atggataatc
    22081 gatattgata aatctgaatt aaataacctt ggtgttaatt ggcaaggaac ggcttcattt
    22141 ggtgattcgt ttggtgcgtc atttaatatg agctcatcag catcgataag cactttggac
    22201 ggcaataagt ttattgcttc agttatggca ttaaatcaga aaaagaaagc aaatgtagtt
    22261 tcaaggccgg tgattttgac acaagagaat attccggcta tatttgataa taacaggact
    22321 ttttatgtgt cattagttgg tgagcgcaat tcttcgttgg agcatgttac gtatggaaca
    22381 ttgataaatg ttatacctcg cttttcatct cggggacaaa ttgagatgtc tttaacgata
    22441 gaggatggaa caggaaatag tcagtcaaat tacaattata ataatgaaaa tacatctgtc
    22501 cttccagaag ttggaaggac caaaatcagt acaatagcga gagttccaca aggcaaaagt
    22561 ttgttgatag gtggttatac gcatgaaacg aactctaacg aaatagtatc tattcctttt
    22621 ctctcctcta ttcctgtaat cggtaatgtg ttcaagtata aaacaagtaa tatcagtaat
    22681 attgtaaggg tttttctgat tcaaccccgt gagataaagg agtcaagtta ttataatact
    22741 gcagaatata aaagcctcat atctgaaaga gaaatacaaa agactacaca aataatccca
    22801 tctgaaacta ctttgctgga agacgaaaaa tcattggttt catacttaaa ttactaacta
    22861 taaagtaggt gatgtatgct tgatgttaaa aatacaggag tttttagctc tgcattcatt
    22921 gataagttga atgcaatgac aaattcagat gatggagatg agactgctga tgcagagctt
    22981 gattctggct tggctaatag caagtatatt gactcatctg atgagatggc ttccgctctt
    23041 tcgtcattta taaacagaag agaccttgag aaactgaaag gaacaaatag tgatagtcag
    23101 gaacgtattt tagatgggga agaagatgaa attaatcaca agatttttga tttaaagaga
    23161 acgttaaaag ataaccttcc tctagatcgg gattttatag acagactaaa gagatatttt
    23221 aaagatccaa gtgatcaagt cttagcatta agggaacttt tgaatgaaaa agatcttact
    23281 gctgaacaag tcgaattatt aactaaaatt attaatgaga taatatcagg tagtgaaaaa
    23341 agtgttaatg ctggaataaa ttcagctata caggctaaat tatttggcaa caaaatgaaa
    23401 cttgaaccac agcttttgcg tgcatgttat cgtggtttta tcatggggaa catatcaaca
    23461 acagatcagt atatagaatg gcttggtaat tttggtttta atcacagaca tacaattgtg
    23521 aattttgtag agcagtcact gattgtagac atggattctg agaaaccgag ctgtaatgct
    23581 tatgagtttg gttttgtgtt atctaaatta attgcaatta agatgattag aacttcagac
    23641 gtaattttta tgaagaaact ggaatcctca agcttgctaa aagatggcag tttaagtgca
    23701 gagcagctat tgctaacttt attatatatt tttcaatatc caagtgaaag tgagcaaatt
    23761 cttacttctg ttatagaagt atcacaagcc agtcatgagg attctgtagt gtatcaaaca
    23821 tatctatctt ctgttaatga aagtcctcat gatatattta aaagtgaaag tgaaagagaa
    23881 attgcgatca atattctacg agagcttgtc acaagtgcat acaagaaaga gctttctaga
    23941 taacaggaga taaaagtgat ccagtctttt cttaagcaag taagtactaa gcctgaatta
    24001 attatattag tgcttatggt tatgatcatt gctatgttga tcataccatt acctacatac
    24061 cttgttgatt ttttaatagg gcttaatatt gtacttgcta ttctcgtttt tatgggatca
    24121 ttttatattg aaagaatatt gagtttttct acattccctt cggttttgtt gataacgaca
    24181 ttatttcgtc tcgctttatc aataagtact agtcgactaa ttttagttga tgcggatgcg
    24241 ggtaaaatta ttactacatt tggccagttt gtcattggtg atagtctggc tgtaggtttt
    24301 gttatttttt caatagtaac tgttgtgcag ttcattgtta taacaaaagg atctgaaagg
    24361 gttgcggagg tagctgctcg cttttctctt gacggtatgc cggggaaaca aatgagcatt
    24421 gatgcggatt taaaggctgg aattattgat gccgcaggag ctaaagaaag acgcagtatt
    24481 ctggaacgtg agagtcaatt gtatggttca tttgatggag caatgaagtt cattaaaggt
    24541 gatgcaattg ctggcatcat tattattttt gtcaatttaa taggaggtat ttctgttggt
    24601 atgagtcaac atggaatgtc cctatctggt gcattatcta cttataccat actaactatt
    24661 ggtgacggat tggtctctca gattcctgct ctgttaattt ctataagtgc aggatttatc
    24721 gttacaagag ttaatggtga tagcgataat atgggacgta acataatgtc ccagatattt
    24781 gggaatcctt ttgttctcat tgttacatca gctcttgcct tggcgatagg gatgttgcca
    24841 ggttttccgt tttttgtttt tttcctgata gcagttactt tgacggcttt attctattat
    24901 aaaaaggtcg tagaaaaaga aaaaagtctg tctgagtctg attctagtgg ctatactggt
    24961 acatttgata ttgataatag ccatgactca tctttggcaa tgatagaaaa tctggatgct
    25021 attagttcag aaaccgttcc tttaatttta ttatttgccg aaaataagat aaatgcaaat
    25081 gatatggaag gtcttattga aaggataaga agtcagttct ttattgatta tggtgtcagg
    25141 cttccaacta ttttatatag aacaagcaat gagcttaagg tcgatgatat tgttttgtta
    25201 ataaatgagg tgcgtgccga tagttttaat atatattttg acaaagtgtg tattacagat
    25261 gaaaatggag atatagatgc tctaggtatt cctgttgttt caacttcata taatgaacgt
    25321 gttatttctt gggtagacgt ttcatataca gaaaatctaa ctaatattga tgctaaaatt
    25381 aaaagtgctc aagatgaatt ttatcaccag ttgtcacaag ctttattaaa caacataaat
    25441 gagatttttg gtatacaaga aacaaaaaat atgttagatc agtttgaaaa tcggtatcct
    25501 gatctattaa aggaagtctt ccgacatgtg actatacaga gaatttctga ggtattacaa
    25561 agattgcttg gagaaaatat ttctgttcgc aatttaaaac ttattatgga gtctttggcg
    25621 ctttgggctc ccagagaaaa agatgtcata acattagttg aacatgtccg tgcatcactt
    25681 tctaggtata tttgtagtaa aatagctgtt tctggtgaga ttaaagttgt gatgctttcc
    25741 ggatatattg aggatgcaat aagaaaaggg ataaggcaaa cctctggtgg ctctttcttg
    25801 aatatggata tagaggtttc ggatgaggta atggaaactt tagcacatgc tttgagagaa
    25861 ttgagaaatg caaaaaaaaa tttcgttctt ttggtatcag tagatatacg taggtttgtt
    25921 aaaagactta tagataacag atttaagagt atactcgtta tatcgtatgc tgagattgat
    25981 gaagcatata ccattaatgt attaaagact atttagtgag gtttaaatat gagtaacatt
    26041 aatttagttc aattagttag agatagtctt ttcacgattg gttgtcctcc atcaattatt
    26101 acagatttag acagccattc agcgattacc atttctttgg atagtatgcc tgctattaat
    26161 atagctcttg tcaatgagca ggttatgtta tgggctaatt ttgatgcacc aagtgatgta
    26221 aaacttcagt catctgctta taacatatta aatttaatgt taatgaactt tagttatagc
    26281 attaatgagc tggtagagct tcatcgttct gatgagtact tacagttaag agttgttatt
    26341 aaagatgact acgttcacga tgggattgtt tttgcagaga ttctgcatga gttttatcag
    26401 aggatggaaa tcttaaatga ggtcttataa tcaatgagct atacaaaatt gctcactcaa
    26461 ttatcttttc ctaatagaat ctcggggcca atcttggaaa caagtcttag cgatgtttcg
    26521 attggtgaga tttgtaacat tcaggctgga attgaaagta atgaaattgt tgcaagagct
    26581 caggttgtag gatttcatga tgaaaaaaca atattaagct tgattggaaa ttctcgtgga
    26641 ctttcacggc aaacgttgat taagcccact gcccagtttc ttcatacgca agtcggccgt
    26701 ggattattgg gagcagtagt caatccttta ggggaggtta ctgataagtt tgctgttacg
    26761 gataacagtg aaattcttta tcgacctgta gataatgctc ctccgctata tagtgaaagg
    26821 gctgcaattg agaagccttt tttaacaggt attaaggtta ttgattcttt actcacgtgt
    26881 ggtgaaggac agcgaatggg gatttttgcg tcagctggtt gtggcaaaac ttttctcatg
    26941 aatatgctca ttgaacatag tggtgctgat atatatgtta ttgggttaat tggtgagcga
    27001 ggtcgagagg ttacagaaac ggttgattat ttgaaaaact ctgagaaaaa aagcaggtgt
    27061 gttttagtat atgcaacttc ggattactct tcggttgatc gttgtaatgc tgcatatata
    27121 gccactgcta tagccgaatt ttttaggact gaaggacata aagtagcgct ttttattgat
    27181 tcattaacaa ggtatgccag agcattacgt gatgtggcct tagccgctgg agaatcacct
    27241 gccagaagag gctatccggt ttcggttttt gatagcttac ccagacttct tgaaaggcca
    27301 ggaaagttaa aggcaggtgg ctctattact gcattttaca ctgttctttt ggaggatgat
    27361 gattttgctg atccattagc tgaagaggta agatccattt tagatggaca tatatatttg
    27421 agcagaaatc tagcccaaaa aggacaattt cctgcaattg attccttaaa aagtataagc
    27481 agggtattta cacaggttgt tgatgaaaaa catcgtatta tggccgctgc atttcgggag
    27541 ctactttctg aaatagaaga gctaaggaca attattgact ttggtgaata caaaccgggg
    27601 gagaatgcct ctcaggataa aatctacaac aaaatatctg ttgttgagag ttttctgaag
    27661 caagattatc gactcggttt tacttatgag cagacaatgg agcttattgg tgaaacaatt
    27721 agataaggtt ttaaaaatca aagataaata tcaacggtct gttaaattaa ttgaagcaca
    27781 catattaaca ctaaaaaaaa aactctttgt atcgagatgt ggaggcatta gataaaagga
    27841 ttatatactt cctgcaactg gagaatgact tagaacctgt aggcgctcag agcgttagtc
    27901 agcttttcaa tacaagacgt aagatcgcta tagtaaaaaa gcatattatc caatatcagt
    27961 ctgaacgaat tcttctaaag ggaagaattg aagaaattca aaaagatatt gatgaggcaa
    28021 atgcttctaa aagaaaactg cttcataaag agagtaaaat atgtaaacgg attggcttga
    28081 ttaaaagaaa taactttgcc aaacagttga tacttgatga gttatcacaa gaggatatga
    28141 agtatggcat tagacaatat aaacctaaat tttagtagtg acaaacagat agaaaaatgt
    28201 gagaaactat cttcaataga taatattgat agtctcgttt tgaagaagaa gaggaaggtt
    28261 gaaattcctg agtactcttt aattgcagct aactatttta ctattgataa gcactttgaa
    28321 cataagcatg ataaaggaga aatttatagt ggcattaaaa atgcgttcga acttagaaac
    28381 gaacgagcga catattctga tattccagaa tcaatggcca ttaaagaaaa tattttgata
    28441 ccagatcaag atatcaaagc aagggaaaaa ataaatatcg gcgatatgag ggggatcttt
    28501 tcatataata agagtggaaa tgcagacaag aacttcgaaa gaagtcatac ttcttctgta
    28561 aaccctgata atctgctaga atctgataat agaaatggtc aaattggttt aaaaaatcat
    28621 agcttgtcta ttgataagaa tattgctgac atcatttctt tactaaatgg aagtgttgct
    28681 aaatcatttg agctccctgt aatgaataaa aatactgcag acataacccc atccatgtca
    28741 ttgcaagaaa aatcaatagt tgaaaatgat aaaaatgttt ttcaaaaaaa tagtgaaatg
    28801 acttaccact ttaaacagtg gggggctgga cattctgtta gtatttcagt ggagtctggt
    28861 tcttttgttc taaaaccgtc agatcaattt gtaggaaata aacttgactt aattttgaaa
    28921 caagatgctg agggtaatta cagatttgat agcagtcaac ataataaggg gaataaaaat
    28981 aatagtacag gatataatga acagagtgaa gaagaatgct aagaattaaa cattttgacg
    29041 ctaacgaaaa actacagatt ttatatgcaa agcaactctg cgagcgtttt tcgattcaga
    29101 cattcaaaaa taaatttaca ggcagtgaaa gtttagtcac tcttacttcc gtgtgtgggg
    29161 attgggtaat tcgtattgat acattatctt ttttgaaaaa aaaatacgag gtattttcag
    29221 gattttctac acaagaatct ttactgcatt tatcaaaatg tgtctttata gagtcgtcat
    29281 ctgtattttc gattccagaa ctgtctgata agattacttt ccggatcacg aatgaaatcc
    29341 agtatgcaac tactgggagt catttatgct gtttttcctc ttctttaggt attatttatt
    29401 ttgacaagat gccggtatta cgtaatcaag tttctcttga cttattgcat catcttttag
    29461 agttttgctt aggttcatct aatgtaaggc tggctacttt aaaaagaatt cgcactggtg
    29521 atataatcat agttcagaaa ctttataatt tattattgtg taatcaagtt attattgggg
    29581 attatattgt gaatgataat aatgaggcaa aaattaatct gtcagaaagt aatggtgagt
    29641 cagaacacac agaagtttct ttggcattat tcaattatga tgatatcaat gtaaaagtgg
    29701 actttattct tttagaaaaa aatatgacaa tcaatgaact aaaaatgtat gtagaaaacg
    29761 aattattcaa gtttcccgat gacatagtta aacatgtaaa tattaaagta aatggttctt
    29821 tggttgggca tggggaactt gtttctattg aggatggtta tggtatcgag attagttctt
    29881 ggatggtaaa ggagtaatct catgctgagt gacatgtccc tcatagctac actttctttt
    29941 tttacgctat taccattttt agttgctgct ggtacctgtt atataaagtt ttctattgtt
    30001 tttgtaatgg tacgaaatgc acttggtttg cagcaggttc catcaaatat gactctcaat
    30061 ggtattgcat tgattatggc tttgttcgtg atgaaaccca ttattgaagc tggttatgaa
    30121 aattatctaa atggccctca aaaatttgat acaatatctg atattgtccg tttttctgat
    30181 tctggcttga tggaatataa gcaatacttg aaaaagcata cagatcttga gttagcacgt
    30241 tttttccaaa ggtctgaaga agaaaatgct gatttaaaga gtgcagagaa taatgattat
    30301 tcattgtttt ccctcttgcc tgcatatgcg ctaagtgaaa tcaaagatgc gttcaaaata
    30361 ggtttttatt tatatttgcc atttgttgtt gttgatcttg ttatttcaag cattttactt
    30421 gcacttggta tgatgatgat gagtccaatc acaatatcag taccaataaa acttgtatta
    30481 tttgttgcgc ttgatgggtg gggtatcctg tctaaggcct tgattgaaca atatatcaat
    30541 attcctgctt agatgatgga ggtaatgtgt ctgatatagt ttatatgggt aataaggctc
    30601 tttatttaat ccttatcttt tccttatggc cagtaggtat agctacggtt attggattat
    30661 ctattggttt gttacagaca gtgactcaac tccaagagca gacacttcct tttggtataa
    30721 agcttatagg tgtctcaata tctttgctac ttctttctgg atggtatggt gaggttttat
    30781 tgtctttttg tcatgaaata atgtttttaa ttaagagtgg ggtttgatgg acatttcaag
    30841 ctggttcgaa agtattcatg tgtttttaat actcctgaac ggcgtttttt ttagattggc
    30901 tccattattt ttctttcttc catttttaaa taacggtata atttctccat ctattagaat
    30961 acctgtgatt tttcttgttg catcaggatt aattacttct ggtaaggtag acataggttc
    31021 ttctgttttt gaacatgttt atttccttat gttcaaggaa ataattgttg gcctccttct
    31081 ctctttttgc ttgtctcttc ccttttggat atttcatgct gttggtagca ttattgacaa
    31141 ccagcgtggg gcaacgctta gtagttcaat tgatcctgcc aatggtgttg atacgtctga
    31201 gttggcaaaa tttttcaatc ttttttctgc agttgtattt ctatacagtg gtggtatggt
    31261 ctttatttta gaatccatac aattgtctta taatatatgc ccgttatttt ctcaatgttc
    31321 tttccgtgtc tcaaatatct taacatttct gactttattg gcaagtcagg ctgttatttt
    31381 agccagtcct gttatgatag tattgttact atcagaagta ttacttggtg tattatcgag
    31441 atttgctccg cagatgaatg ctttttccgt atcattaact attaaaagtt tacttgcaat
    31501 atttattatt ttcatctgtt cttctactat ttacttttct aaagttcaat ttttcctcgg
    31561 tgaacataaa tttttcacaa atctatttgt tagataaaat attatggcaa ataaaacaga
    31621 aaagccgaca cctaaaaaac taaaggatgc cgcaaaaaaa ggacagtcat ttaaatttaa
    31681 ggatttaacg actgttgtta ttattctggt agggacattt actataatat cattcttttc
    31741 cttaagtgat gtaatgctct tatacagata tgtaataatt aatgacttcg aaattaatga
    31801 gggtaaatac ttttttgcag tggttattgt cttttttaag ataattggct tcccactttt
    31861 tttctgtgtt ctttcggctg tgttgccaac attggttcaa acaaagtttg ttcttgcgac
    31921 taaagctatc aagattgatt tttcagtatt aaaccctgtt aaagggttaa aaaaaatatt
    31981 tagtataaag acaataaaag aatttttcaa aagcattctg cttcttatta ttctagcatt
    32041 aacaacctat ttcttttgga ttaatgaccg aaaaataatt ttttctcagg tgttttctag
    32101 tgttgatggc ttatatctta tttgggggag gctgtttaag gatataatat tatttttctt
    32161 ggcattttct gtttttgtta ttattcttga ctttgtgatt gagttcattt tatacatgaa
    32221 agatatgatg atggataaac aggagataaa aagagaatat atagagcaag agggacactt
    32281 tgagacaaag tcgagaaggc gtgagttgca tatcgagatt ctttcagagc agactaaatc
    32341 tgatatacgt aattcaaaat tagtggtaat gaacccgact catattgcaa ttggtattta
    32401 ttttaatcca gaaatagcgc ctgcaccttt tatttctctc attgaaacta accagtgtgc
    32461 cttggctgtc agaaaatatg caaatgaagt tggtataccg actgtgcgtg atgtgaaatt
    32521 agctagaaaa ctatataaaa cacatacaaa atatagtttt gttgattttg aacacttgga
    32581 tgaagtccta cgtcttattg tttggcttga gcaggttgaa aacactcatt aataagtaaa
    32641 ggagatgtgt tatatgggag ttaatttctg taataaaata ggtattgatc agtctgaatt
    32701 tgaaatagaa tcttccatca ttaactccat tgctaatgag gtattgaacc caatatcttt
    32761 ccttagcaat aaggatataa taaatgtttt actcagaaag atttcatctg aatgtgacct
    32821 ggttagaaaa gacatttatc gttgtgctct ggagttggtc gttgaaaaaa cacctgatga
    32881 tttataatca ttcataataa ctcttttgct gatataaata tttttgttga agatatttat
    32941 gtagtcacag gatcaattgt agaaaattgt tatgtttttg ttaggaactg cactaaataa
    33001 tatatgataa gacagcaaaa acgactgacc ataatccttc ttttattagg agttgataag
    33061 agagactatt catcctgtaa tgttaaaact ctgttatata gtattcgcga ttatgcaaag
    33121 tctgtaaatg atcatgagat actaaccgag agtaatcgtt tattatctca ttgcatttca
    33181 gatagtaacg gagctttctt taaaagtagt aaatacgttc cccttaagta cttacgtaaa
    33241 aggagaatag cacgaaagat accaaatgat tagcactttg ctagtaagct tgctggtaaa
    33301 ctatattgac cgttgggttt gttttgagac tgtaatttaa attgtgtaat tgcctgtttt
    33361 tgatatgttt actccaacaa cggagacagg taaattatgg acgaaaagaa gcttaaagcc
    33421 cttgcggctg aactggctaa aggcctcaaa actgaggccg acctcaatca gttttcccgt
    33481 atgctgacga aatatcccgg ctcttaaaac gatagtggct caggctttca tttttcagcg
    33541 ttccc
//
LOCUS       DAJTYF010000030        33129 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTYF010000030 DAJTYF010000000
VERSION     DAJTYF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.030"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..224
                     /locus_tag="OO590_001582"
     CDS             <1..224
                     /locus_tag="OO590_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCR6636988.1"
                     /translation="PQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVL
                     DGKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            398..1167
                     /locus_tag="OO590_001583"
                     /pseudo
     CDS             398..1167
                     /locus_tag="OO590_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1200..2720)
                     /locus_tag="OO590_001584"
     CDS             complement(1200..2720)
                     /locus_tag="OO590_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6636989.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNERAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            2811..3530
                     /gene="tonB"
                     /locus_tag="OO590_001585"
     CDS             2811..3530
                     /gene="tonB"
                     /locus_tag="OO590_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6636990.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(3570..3968)
                     /gene="yciA"
                     /locus_tag="OO590_001586"
     CDS             complement(3570..3968)
                     /gene="yciA"
                     /locus_tag="OO590_001586"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6636991.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4073..4612)
                     /locus_tag="OO590_001587"
     CDS             complement(4073..4612)
                     /locus_tag="OO590_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6636992.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(4642..5385)
                     /locus_tag="OO590_001588"
     CDS             complement(4642..5385)
                     /locus_tag="OO590_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6636993.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(5413..5502)
                     /locus_tag="OO590_001589"
                     /pseudo
     CDS             complement(5413..5502)
                     /locus_tag="OO590_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5742..6380
                     /gene="ompW"
                     /locus_tag="OO590_001590"
     CDS             5742..6380
                     /gene="ompW"
                     /locus_tag="OO590_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6636994.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(6440..6946)
                     /locus_tag="OO590_001591"
     CDS             complement(6440..6946)
                     /locus_tag="OO590_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6636995.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDCVEAKK"
     gene            complement(6992..7492)
                     /locus_tag="OO590_001592"
     CDS             complement(6992..7492)
                     /locus_tag="OO590_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6636996.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(7578..7757)
                     /locus_tag="OO590_001593"
     CDS             complement(7578..7757)
                     /locus_tag="OO590_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6636997.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(8138..8944)
                     /gene="trpA"
                     /locus_tag="OO590_001594"
     CDS             complement(8138..8944)
                     /gene="trpA"
                     /locus_tag="OO590_001594"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6636998.1"
                     /translation="MERYESLFAQLKERKEGAFIPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(8944..10137)
                     /gene="trpB"
                     /locus_tag="OO590_001595"
     CDS             complement(8944..10137)
                     /gene="trpB"
                     /locus_tag="OO590_001595"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6636999.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(10149..11510)
                     /gene="trpCF"
                     /locus_tag="OO590_001596"
     CDS             complement(10149..11510)
                     /gene="trpCF"
                     /locus_tag="OO590_001596"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6637000.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(11511..13106)
                     /gene="trpD"
                     /locus_tag="OO590_001597"
     CDS             complement(11511..13106)
                     /gene="trpD"
                     /locus_tag="OO590_001597"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6637001.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(13106..14668)
                     /gene="trpE"
                     /locus_tag="OO590_001598"
     CDS             complement(13106..14668)
                     /gene="trpE"
                     /locus_tag="OO590_001598"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6637002.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDNALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(14690..14782)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            14941..15822
                     /gene="yciV"
                     /locus_tag="OO590_001599"
     CDS             14941..15822
                     /gene="yciV"
                     /locus_tag="OO590_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6637003.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            15819..16439
                     /locus_tag="OO590_001600"
     CDS             15819..16439
                     /locus_tag="OO590_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6637004.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            16540..17412
                     /gene="rluB"
                     /locus_tag="OO590_001601"
     CDS             16540..17412
                     /gene="rluB"
                     /locus_tag="OO590_001601"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6637005.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(17452..18042)
                     /gene="cobO"
                     /locus_tag="OO590_001602"
     CDS             complement(17452..18042)
                     /gene="cobO"
                     /locus_tag="OO590_001602"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6637006.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(18039..18797)
                     /locus_tag="OO590_001603"
     CDS             complement(18039..18797)
                     /locus_tag="OO590_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6637007.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            19017..20066
                     /gene="sohB"
                     /locus_tag="OO590_001604"
     CDS             19017..20066
                     /gene="sohB"
                     /locus_tag="OO590_001604"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6637008.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKETKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(20102..20353)
                     /locus_tag="OO590_001605"
     CDS             complement(20102..20353)
                     /locus_tag="OO590_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6637009.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            20733..23330
                     /gene="topA"
                     /locus_tag="OO590_001606"
     CDS             20733..23330
                     /gene="topA"
                     /locus_tag="OO590_001606"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6637010.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPSKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            23540..24514
                     /gene="cysB"
                     /locus_tag="OO590_001607"
     CDS             23540..24514
                     /gene="cysB"
                     /locus_tag="OO590_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6637011.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            24809..24973
                     /locus_tag="OO590_001608"
     CDS             24809..24973
                     /locus_tag="OO590_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6637012.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLTVFLGSALFWVV
                     VALLIWKVWG"
     gene            24976..25143
                     /locus_tag="OO590_001609"
     CDS             24976..25143
                     /locus_tag="OO590_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637013.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            25257..25352
                     /locus_tag="OO590_001610"
     CDS             25257..25352
                     /locus_tag="OO590_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637014.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            25516..28191
                     /gene="acnA"
                     /locus_tag="OO590_001611"
     CDS             25516..28191
                     /gene="acnA"
                     /locus_tag="OO590_001611"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6637015.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(28255..28845)
                     /gene="ribA"
                     /locus_tag="OO590_001612"
     CDS             complement(28255..28845)
                     /gene="ribA"
                     /locus_tag="OO590_001612"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6637016.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYADISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            29015..29779
                     /gene="pgpB"
                     /locus_tag="OO590_001613"
     CDS             29015..29779
                     /gene="pgpB"
                     /locus_tag="OO590_001613"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6637017.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWTLVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            29928..30236
                     /gene="lapA"
                     /locus_tag="OO590_001614"
     CDS             29928..30236
                     /gene="lapA"
                     /locus_tag="OO590_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6637018.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            30243..31412
                     /gene="lapB"
                     /locus_tag="OO590_001615"
     CDS             30243..31412
                     /gene="lapB"
                     /locus_tag="OO590_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6637019.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            31605..32342
                     /gene="pyrF"
                     /locus_tag="OO590_001616"
     CDS             31605..32342
                     /gene="pyrF"
                     /locus_tag="OO590_001616"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6637020.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNAHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            32342..32668
                     /gene="yciH"
                     /locus_tag="OO590_001617"
     CDS             32342..32668
                     /gene="yciH"
                     /locus_tag="OO590_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6637021.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(32794..33012)
                     /gene="osmB"
                     /locus_tag="OO590_001618"
     CDS             complement(32794..33012)
                     /gene="osmB"
                     /locus_tag="OO590_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6637022.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVISHQVGK"
BASE COUNT         8399 a         8004 c         8421 g         8305 t
ORIGIN      
        1 ttccacagtt ttcacctgaa gcattaaata atctgaaagg tcgagtggaa acattcacac
       61 aagatttaaa ggttatcaaa ccgtcagtaa atattcagcc tcagacaatc aatatcacaa
      121 ctcagacggt acttgatgga aaggttattg atgaaagaac cacatctcat attaatcgta
      181 tgcaggaaga taccttgatt agctcagctt acccagagga ataaacaatg ttagaactac
      241 taatatttat tgccacacat ggcaatctat taatctttat ctagtatgac gattcaacta
      301 tgaatagtcg aatgctacgt gatttcttct gagcagatac caacatcaaa aattagattt
      361 cacgtagctc acgcttaaca gaataggaat aacaacaatg tttgacacaa agcaaattag
      421 atactcaggt aaagatggta tctattttca ccttagagat aatgtagaat cttttcttgc
      481 cttaacagct actgaacaga tggactatga ctcaccgatg atggtgacaa ccaggctatg
      541 caatcggggc aaactatcac tgataatgtt cagagagcac ctaagaccat ctctattagc
      601 ggtgttgtag tggttgattc gacgggttca gttctattaa ccagacaggg gcaacttgta
      661 gagaccttta tttctacggt tgaacaatgg cgagaccaga agcaaatcat cactattgtt
      721 tgtaaagatg gaatatctat cactgatagt atcattactc agtttaaagc gagtaaagag
      781 cataccattg ctaatggtct tcgtattcag atgaccttcc aaggtgtaga atttcgttca
      841 atcgtagggc aaactgatat ttcagcagct actggcaaga ccgctaccac gaatgatggt
      901 ggtgctacca gtaaaaagaa cactggcaac actacaacga gtcttggaag ccctatgctg
      961 acctgtaaag agctttttag ctattcggca agtgagttat cagatgaagc gctaaaagcc
     1021 cgtgtaacgt gctctaagag cgttagcgtt aaaaatggtg agagcacctt cacagcctca
     1081 gctaatgagc gagcttcaaa agtgcttaag tcgggtaatg ctttacggaa gtactctgtg
     1141 aatccgaata agaagggcac ttattgagac ataaaaaggg gcttcatagc ccctgaaaat
     1201 tacagttgat aagcatcaat atccagtagc tctttatcag caatttcaaa gattggtgac
     1261 ccatcattta gcggtagcgg gtctgtaggt atggtgattt cagtgtcgtt ctgtagcgtt
     1321 atagttactg aacccttacc caatgtaagc ccttttagat gtttggacaa aattaattga
     1381 cactcgacac gacccgcttt agttgtcagg tcaatatctt taagctgggg tagttcctta
     1441 tcagtgagtg aagccttacg cttcatagta tcaatcgtgg tctgtagttc tgtgcgcttc
     1501 tggttaagtt ccttaagctg aatttttagt tcatcaatat catccatctc aagaagtagg
     1561 gcggtagctt tcttgattct ggctgtgaca atttcaacct catgctgtaa cgctgcaatc
     1621 ttcctctctg attctccatc attccccacg tcaatcatcc ccagaaaatt atcaatcatc
     1681 catcggtcaa acggtttacg cttcaacggt ggaacatcac agcgaccgtc taagtggtta
     1741 ttgcacctgt agaccccagc atacttattc tttgttccgg taggatggac actagcccca
     1801 cactgacacc taaacagacc tttgaagatg ttaatcgtta gtgggttgtc acttgcaggt
     1861 gtacggcctt tacgattctt atcaagaatc tcttgagctt tattgaacgt tgcagcatct
     1921 atagcggctt cgtagtagtt cggtatagcg gggcggtcat tgcgctgtga tgctggtagt
     1981 gtacctatcg ttgtctctga ccttaagata gtacgcacag aatgaacgct aaatccatta
     2041 gcatctttac gtcgtgagaa tgaactaatg ttattagcct gtaggtgacg tgcaatgtta
     2101 tgcagacttt caccatctag cagcagcttg aaaatcaagc gtacagtttc agcacgttca
     2161 ttgagtttaa aagtctcagg gtcaatccat ccgggaacat tccttgttaa acgttgttca
     2221 ccagcaaggg caagctttgc acgttgttcc catttctgac taacacggaa agacttctgt
     2281 ttactctcct gatgcgctat gtagctttta acccgctgaa tgattgagat tcctaaatca
     2341 tcggatagag catcccttgt gtagattagc ccatcctgta gagtgtgaat ctcgatacca
     2401 tgagcaataa tgcgattcat caaggcttct gtttcaagat gttcaaggcg gcctatgcgg
     2461 tcgatagctt cgactagtaa aattgtccct tctggaaact cccctgattc aatgcgggag
     2521 agcatatccc caaattgccc tgataagtgt ttacctttgt aaccggatag accttcatca
     2581 ttgaaagatg ccactacctg agcatcgcta ttcttcctta accactgttt gaatagctca
     2641 ttttgtcgtc tggttgaatc agcaccttca gcaccttgtg tggctgatga gaatctcaag
     2701 tatgttatta cggttctcac ggtgataaac tcccatgcaa ttgattatca tttaaagatg
     2761 aacttataaa cgttatggct aatgctacag tgccgaatag ctacaataca atgccccttg
     2821 atttacctcg ccgcttcccc tggccgacgt tactttcggt ctgcattcat ggtgctgttg
     2881 tggcgggtct gctctatacc tcggtacatc aggttattga actacctgcg cctgcgcaac
     2941 caatttctgt cacgatggtt gcgcctgctg atctcgaacc gccacaagcc gttcagccgc
     3001 caccggagcc ggtggtagag ccagaaccgg aacctgagcc gatccccgaa ccgccaaaag
     3061 aagcaccggt ggtcattgaa aagccgaagc cgaaacctaa gccaaaaccg aagccggtga
     3121 aaaaggtaca ggagcagcca aaacgcgatg tcaaacccgt agagtcgcgt ccggcatcac
     3181 cgtttgaaaa tacggcaccc gcgcgcccca catcaagtac ggcaacggct gcaaccagca
     3241 agccggttac cagtgtggct tcaggaccac gcgcattaag ccgtaatcag ccgcagtatc
     3301 cggcacgagc acaggcattg cgcattgaag ggcaggttaa agttaaattt gacgtcacgc
     3361 cggatggtcg cgtggataac gtacaaatcc tctcagccaa gcctgcgaac atgtttgagc
     3421 gtgaggtgaa aaatgcgatg cgcagatggc gttatgagcc gggtaagcca ggcagtggga
     3481 ttgtggtgaa tatcctgttt aaaattaacg gcaccaccga aattcagtaa gcagaaagtc
     3541 aaaagcctcc gaccggaggc ttttgactat tactcaacag gtaaggcgcg aggttttcct
     3601 tcaggatcaa ccgcgacata cttaaataat gcttctgtcg ctttatagcg ttgcccgatt
     3661 ggttcagacg ctactttttt cacccacact tcaatattaa tgctgaccga tgtcgtccct
     3721 ttctggacac agcgtgcata gcagcacacc acatcgccga ccgcaaccgg ccgtaagaaa
     3781 gtcattcctt caacccgcac agtcactacg cgaccgtggg caatttcttt tgccagaata
     3841 gcgccgccaa tatccatttg tgacattaac caaccaccaa agatgtcacc attggcattg
     3901 gtatcggcgg gcatggctaa agtacgtaaa acaagatcgc cctgagggac gttatgtgtt
     3961 gtagacatgg taaaaccgac ttaaaaggaa aatcacaggc gcgatgctac tatgattttc
     4021 ctgaggagaa cagaggagat acagcgccag ccccgaagga ctggcagtct ggttaggatt
     4081 tatcttcctg cggcatgtgg cggtagatat agataccgct taacaatgtg aagattaagg
     4141 taagggcggt caggccaaag actttaaagt tgacccaaat attttgcggc agccagaatg
     4201 cgatgtagat gtttgccaga ccgcaaagga taaagaaaac agcccaggcc agatttagct
     4261 tcgaccatac cggttgcggc agcgtgagtt ctttacccag catccgctga attagcggct
     4321 ttttcatcac ccattggctg actaacaggg ctaccgcaaa cagagcataa atgactgtaa
     4381 ccttccattt aataaactca tcattgtgga agaacagcgt caagccaccg aagacgacca
     4441 ccagaacaaa agtgatcagg gccatcttct caaccttacg aaagcgaacc cagctataga
     4501 taagcacaat cgccgtggcg acgatcagcg ctgcagtagc cgcatagatg tcataaatct
     4561 tgtaaaacgc gaaaaagaca accagcggta aaaaatcaag aaactgcttc attttacgat
     4621 tccgtaatca aatgcttaag gttattggcg aatcaacata tacaggcgga acagatagat
     4681 gagcaatatg gcagaaatca ggttgctcaa ggtgttcgcc agtacggcac caatttccgg
     4741 ggttaatgcg gcaaaagaag aggcaaaaag cagcaacagt gtttttgcca gcaaccagct
     4801 cagtactgcg ggtgccacca gacgcatatt cgcccaagtc agccgcatac tgctacgcat
     4861 cgaggcaaaa atgcccattt tgtcctgtac cagcatcacc ggtgccaggg ccagtaaaat
     4921 ggccatgata attcccggaa cgaccaccag catgatgcca atctgtacta aaagggtagt
     4981 cagaaaaatc agaataaata acttgggcaa tatcggcgca ctggcaccaa tagctcgcag
     5041 cgcacagact ctctgacccg cagacaccag ctggataatt aatattacgc ctccggcgag
     5101 aatggcgtta ccgattaatt ctgaaaaagt ggacgccgct gaagcctgca gcaaaatttg
     5161 ttgctgttcc ggtgacatat tctgaaccag gtcgaacaac ccactactgc cgctaacggg
     5221 cacgccgtca ttgagctgcg caagctgtgc atcactgggt gagaaaacat gccctaacac
     5281 cactgtgata aacgcacata gcaacgatac caacagaatg gtcataaatt gattacggaa
     5341 gaaatttccg gtgtcacggt atacggactg cgccgtgata gacatgcact ctccttgagt
     5401 tttatgcagg tgttaattag cgggcaattg taccctggtt aagcctcggg tggcagcatc
     5461 aggtcttgta tggaaaagca tatctttgta aagcggaggt aattgctggc cttcaggcgt
     5521 gggcaaaccg cggcaagtgg cgatcgagtt aatatttgcg tagagaaaat atttaaaaat
     5581 tgatttaaat cacattaacc aggattctca atgcaacttc taaattaatc cagatcaata
     5641 aatggtgaat tatcatatgt aatgtgatct atgtaggatc atttgttact ccaatgtagg
     5701 tatattcgtc acgtttttat aaccataacg acggagcgga tatgaaaaag ttaacagtgg
     5761 cggctttggc agtaacaact cttctttctg gcagtgcctt tgcgcatgaa gcaggcgaat
     5821 tttttatgcg tgcaggttct gcaaccgtac gtccaacaga aggtgctggt agtacgttag
     5881 gaagtctggg tggattcagc gtgaccaata acactcaact gggcttgacg tttacttata
     5941 tggcgaccga caacattggt gtggaattac tggcagcgac gccgttccgc cataaaatcg
     6001 gcacccgggc gaccggcgat attgcaaccg ttcaccatct gccgccaaca ctgatggcgc
     6061 agtggtattt tggtgatgcc agcagcaaat tccgtcctta cgttggggcc ggtattaact
     6121 acaccacctt ctttgataat ggatttaacg atcatggcaa agaggcgggg ctttccgatc
     6181 tcagtctgaa agattcctgg ggagctgccg ggcaggtggg ggttgattat ctgattaacc
     6241 gtgactggtt ggttaacatg tcagtgtggt acatggatat cgataccacc gccaattata
     6301 agctaggcgg tgcacagcaa cacgatagcg tacgcctcga tccgtgggtg tttatgttct
     6361 cagcaggata tcgtttttaa ttccgcacaa aaacgacccc gtaatatacg gggtcaataa
     6421 ggacatggta taaagggtat tatttcttcg cttctacgca atcagtttca gaacgaatta
     6481 aaaatttctc agttgtttgc ggaatatgct gaatcagcca gtcggccatt tgcttttcct
     6541 catttaaaat cgcttcgatg atcggaattg agactgtatc accggcattt tttgctgctg
     6601 ctaatagtga ggtgtaacag gcgatttcaa attgctcgaa gacatatccg ctaatagagc
     6661 ctttaactat ttcatcagaa gggaatatac caccgattga ctgcccaagc gcagccattt
     6721 tactcatgga atctttaatg actgaacgtg aaatgtcatt acgatcaaga atagtttcca
     6781 gttgaacaat ctggtttttg gtttcactaa gatgttgttc aatacgagcg cgtagttcag
     6841 gataattatc tatacggctg gccatggatt caagcataga ttcggcttgc ttttccattg
     6901 cgtgggcgtc acgtaaccag tcatgataat gttcaatacg attcatgaaa atgtcctctt
     6961 tataaaatga ctaaaagtta aaattcatat ttcaggcttt attttcggct ttcttattta
     7021 cgttattaag ggccagatca gtcagtttga tgtcggtggc cttttcttct tccagggttt
     7081 ctttcagaag cttcgctgct ttacggtaac ctaattgttc agccagcgtc gctaatgtcc
     7141 cgtaactggc aatctcataa tgctcgactt tctgtgctgc ggcaatcagt gcggcatcac
     7201 gcacttcgtt tttctcggta ctttcgatga cctcattagc ttcttcaata agaccttcca
     7261 ttgccacaca tttcatgcgc ttaattttca gatttgattc tgattccaca acttgatcaa
     7321 ttcgttcaat ctgtccatga gtttcctcga ggtgcgcatg aaaagcctga cttaattttt
     7381 catttgatgt tgctcttgcg agttttgcca gtgcccgggg taattgtttt tctgcgctgt
     7441 aggtatctga aagcagttga ataaatacat cttcaatggt cttcatattc atatttttct
     7501 ccagtgaaat cactgcgagc catacggccc gcagatgcaa ttatctttat cagctgatat
     7561 taataattaa cggtgaatca ggatttgtct gatttattac caccgctttg ttgaccgcct
     7621 tttttacccg cttcagatgc gcgttgcgga tcatttttaa aattaccgcc gctatgctga
     7681 ccgcctttac ggcctgcgtc ggatgccttc tcacggtctt cggcgaaatt tcctgaacca
     7741 ccacgatgtt cggccatgtt atttctcccg ttgcgttgca ttgtttcatt aatatgagtg
     7801 ttgtgtgtcg acactcatta aaattagtcg ctaatgagaa ttagtcaaat taagcgcaac
     7861 gagaagatag agggaaaata tattttgagg aacattctgg atatattaac aattacctga
     7921 ggaataaatg acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt
     7981 gtttgattat attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag
     8041 cgaaacggta aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa
     8101 agttaaaatg ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca
     8161 tcggttgtac aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct
     8221 cgatgatttt aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg
     8281 cttttacctg atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact
     8341 ctttcagctt cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca
     8401 cgcccgctcg tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagca
     8461 ggtcgtcatc ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg
     8521 cctggcggaa gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc
     8581 cgactttctc gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat
     8641 acatcaacag gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa
     8701 aacattgtgc cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg
     8761 ggccatccgc cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt
     8821 caattagcgt atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga
     8881 aaggaatgaa tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt
     8941 ccatcagatt tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc
     9001 gcgaccggaa aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt
     9061 cagcgcatgg gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca
     9121 cagcgttttg aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc
     9181 agtgctgtta agatacgcgt gttgtgggcc gacagacggg aaatccagtc cggcggagat
     9241 ggagtaagat tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa
     9301 ataaatgccc acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg
     9361 accaccaggc tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat
     9421 gccgatggca ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc
     9481 tctttccaga atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat
     9541 ggtcggataa ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact
     9601 gccggaccag tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg
     9661 taccgggatc acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc
     9721 aacgtctttg gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc
     9781 cgacgccacg ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg
     9841 cttcgccagc aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa
     9901 atcttcacgc ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt
     9961 cagtgcggtt ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc
    10021 aggatctttt tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat
    10081 ttgtggcacg tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt
    10141 tcctttcctt aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg
    10201 atgcccggtt gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc
    10261 gcttccacgc agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga
    10321 ccatttaata gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca
    10381 tatttatcaa cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc
    10441 cagatggcaa catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca
    10501 ttaccatgca gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca
    10561 atatcgtgat tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc
    10621 gcctgttcaa cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc
    10681 gcgcccgcgt cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc
    10741 tcacccagca acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa
    10801 ccaatcagaa aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg
    10861 ccggattcgc tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg
    10921 ttgagatcaa tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct
    10981 aatgcaatgg cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc
    11041 agactgtgag cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt
    11101 aataagcagg catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg
    11161 aagtctttac ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag
    11221 ctcccctgaa aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa
    11281 atggcggcaa tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct
    11341 tttttacact ccagaataaa cgccgtgcgt gcaccttgta gcgcatcata aaaatgtcgt
    11401 gtgctcggct gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct
    11461 acccaaatcg ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt
    11521 gccgccagcg cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt
    11581 tgcgcattgg cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca
    11641 gcgacggctg cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca
    11701 cggttttctt caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa
    11761 tcttcagcgg tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc
    11821 ggcgcgtgta atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac
    11881 cccagcacgc gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt
    11941 aacgccagcg gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg
    12001 gttttcagtt gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca
    12061 aagaggaaac atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca
    12121 agattaatac cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg
    12181 ttaccgtgtt tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa
    12241 atattgatac tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc
    12301 gggcgcggga acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc
    12361 gggtgctcac cacgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc
    12421 tcgccacgca ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc
    12481 tgatacagtt tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc
    12541 caggccagcg tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg
    12601 aactggaatc cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa
    12661 tgggcgttga tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc
    12721 accggcagcg ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac
    12781 gctttaccgt gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt
    12841 gcctgatgtc cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt
    12901 tccggcatac aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga
    12961 ttgctcatcg tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc
    13021 accacgttat gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata
    13081 ttatcgagca gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa
    13141 tagcgcgcag tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag
    13201 aatcaaggac tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg
    13261 agcggatcac aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc
    13321 cgtagctgcc gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg
    13381 gcgcaccgct taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat
    13441 cgtgacgcag ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa
    13501 ctttggtgag atcggcgacg tagcggctgc caggggtgca aatgcgtgcc agatcattac
    13561 gggcgagatc aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca
    13621 gttcaatacg gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc
    13681 gtgttccggc aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc
    13741 tttccggcga cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc
    13801 tgggattact ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga
    13861 aacggcgaga tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac
    13921 gcactacgcc accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg
    13981 aaaccactgg cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag
    14041 cagtgagacg ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc
    14101 tttttttctg atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc
    14161 agttattttc cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga
    14221 acaggccgcc gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca
    14281 ataaacggaa agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg
    14341 gactgacagg ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg
    14401 caggcagggc gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa
    14461 ttgtgacggt gtcacctaat gctgtaatgt gcagcgcatt gtctatcagc agcaggcttt
    14521 ttaaatcatc tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc
    14581 cacacaactg gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca
    14641 gttcgagagt cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc
    14701 cgctcattag gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt
    14761 caggaagtgg ccaccaacct ttcagtacga aaattgcttt cattgtcgat accattttta
    14821 cgtgaacttg cgtactagtc aactggttcg atgattaatt gtcaacagct catttcagaa
    14881 tatttgccag acccgttatg atgtcggcgc aaaaaacatt atccagaacg ggagtgcgcc
    14941 ttgagcgaca cgaattatgc agtgatttac gacctgcaca gccataccac agcttccgat
    15001 ggctgcctga cgccagaagc attggtgcac cgtgcagtcg agatgcgcgt cggcaccctg
    15061 gcgatcaccg accatgacac cacagctgca atcgcgccag caagagagga aatttcacgt
    15121 tcgggactgg cgctgaatct tattcccggc gtggaaattt ccacggtctg ggaaaatcat
    15181 gaaattcata ttgtcgggct gaatattgat attactcatc cgctaatgtg tgagttcctc
    15241 gcgcagcaga cagaacggcg caatcagcgg gcgcagcaaa ttgccgaaag acttgaaaaa
    15301 gcgcaaatac ctggggcgct ggaaggcgca caacgactgg cgcagggcgg cgcagtgacg
    15361 cgcggtcatt ttgcgcgttt ccttgtggag tgcggcaaag ccagttcaat ggcagatgtc
    15421 tttaaaaagt atctggcgcg cgggaaaacc ggatacgttc cgccacagtg gtgtacaata
    15481 gaacaagcta ttgatgtcat tcatcattct ggcggtaagg cggtgctagc tcatcctggg
    15541 cggtacaatc ttagtgctaa atggctgaaa agattggttg cgcattttgc cgaacaccac
    15601 ggcgacgcga tggaagtcgc gcagtgccag caatcgccca atgaacgcac ccagctggcc
    15661 acacttgcgc gtcagcatca tttatgggca tcacaaggat ctgattttca tcagccatgc
    15721 ccgtggatcg aactgggtcg taaactctgg ctgcccgcag gcgttgaagg cgtctggcag
    15781 ctatgggaac agccgcagaa caccacagag agggaattat gagccagttt ttttatattc
    15841 atcctgataa cccgcagcaa cgtctgatca accaggcggt ggagatcgtg cgtaaaggcg
    15901 gggtgattgt ctatccaact gattccggct atgcgctcgg ctgtaaaatt gaagataaaa
    15961 acgccatgga gcgtatttgt cgtattcgcc agctgccgga cggtcacaac tttaccctga
    16021 tgtgtcgcga tctttctgaa ctctcgacct attcattcgt ggataacgtt gcgtttcgtt
    16081 tgatgaaaaa caacacgccg ggcaactata ccttcatcct gaaggggacg aaggaagtgc
    16141 cacgccgtct gttgcaggag aaacgcaaaa ccatcggtat gcgtgtgcct tctaacccga
    16201 ttgcgcaggc gttgcttgaa gcactgggcg aaccgatgct ttccacttcg ctaatgctgc
    16261 caggcagcga atttaccgaa tccgatcccg aagaaattaa agaccgtctg gaaaaacagg
    16321 tagatttgat tattcacggc ggctatctcg ggcagaaacc aacaacggtt attgatctta
    16381 ccgatgatac gccggtcgtg gtgcgtgaag gcgtaggtga tgtgaagcct ttcttataag
    16441 tgcgtaactc tgtatactac gcggccttaa aatggcgcta tacggcgcca ttgattctcc
    16501 tgttcgacgc ctgtgaaggc gacaccaaaa ggaagctcta tgagcgaaaa attacagaaa
    16561 gtgctggccc gcgctggcca cggctcccgc cgtgaaatcg aatctattat tgaagccggt
    16621 cgtgtgagtg ttgatggcaa aattgctaaa ctcggcgatc gcgttgaagt tacccctggg
    16681 ctgaaaattc gtatcgatgg tcatctgatt tcggtacgtg agtccgctga acaaatttgt
    16741 cgcgttctgg cctattacaa accggaaggt gagttgtgta cccgtaacga tccggaaggg
    16801 cgtccgacgg tgtttgaccg tctgccaaag ctgcgtggcg cacgctggat tgccgtaggt
    16861 cgtctggacg ttaacacctg tggtctgctg ctgttcacca ccgatggtga actggcaaac
    16921 cgtttaatgc atccaagccg tgaagttgaa cgtgaatacg ccgtgcgtgt atttggtcag
    16981 gttgacgacg cgaaactgcg tgatttgagt cgtggcgtgc aactggaaga tggtccggca
    17041 gcttttaaaa ccatcaagtt cagcggcggc gaagggatca accagtggta caacgtgact
    17101 ctgaccgaag gccgtaaccg tgaagttcgt cgtctgtggg aagcggttgg tgtgcaggtg
    17161 agccgcctga tccgtgttcg ttacggtgat atcccactgc caaaaggtct gccacgcggt
    17221 ggctggacgg agctggacct cgcccagact aactatctgc gcgaactggt ggagcttccg
    17281 ccagaaacca gctctaaagt cgctgtagaa aaagaccgtc gtcgcatgaa ggctaatcag
    17341 attcgtcgtg cggtgaaacg tcacagtcag gtgagcggcg gtcgtcgttc tggcgggcgt
    17401 aataacggtt aatcaaagta tgccggacgt catatccggc atttttacag attaataatc
    17461 aatccctatt tgcgccttaa caccggcatc aaacgcatgt ctgatggggc gcagctcact
    17521 taccgtatcc gccagttcaa gaatatcccg atgacagcct cgacccgtga taatcaccgt
    17581 ctgttgatgt ggacgttcat ttaacgcctg caccacttct tccagtggca aatagtcata
    17641 cgccaccata tacgtcagtt catcaagcaa aaccatatcc agtgaggaat cagcaagcat
    17701 ccgctttgca tgttgccaga cttcgcggca ggcggcggta tcagactcgc ggttttgtgt
    17761 atcccaggta aagcccgttg ccatcacctg aaactcaacg ccatgtggct ccagcagatt
    17821 gcgttcgcca ttaggccagg tgcctttaat aaactgcacg acgcccactt tttttccgtg
    17881 accaactgcg cgtgttgccg taccaaatgc cgcggtggtt ttgccttttc cattgccggt
    17941 aaagacgatg ataataccgc gttcatcctg ggcctgggcc acacgagcat ctactttttc
    18001 tttcactcgc tgctgacgct gttggtagcg ttcatcactc attgggaaat tcctggttta
    18061 cggcccggtt gggcgtcaaa ggtcatgccg gttttacggc ggctgtcatc gcccatcagc
    18121 cagaggtaga gcggcatgat atcagcgggt gttttaagtt tctgtggatc ttcggtcggg
    18181 aaggcgcttg cgcgcattgc ggtgcgcgta ccgcctgggt taatgcagtt gacgcgcagg
    18241 cgctgctgat attcatcggc cagtacctgc atcatccctt cggtggcaaa tttcgacgct
    18301 gcatatgcac cccagttggc tcgtccctga cgtccaacgc ttgatgaagt aaagaccagt
    18361 gaaccggcgt ccgatttgag taataaagga agcagtgcct gggtgagcat aaaggtggca
    18421 ttaacgttga cctgcatgac gtcctgccag acctgcggat tttgttcgct cattgggcaa
    18481 acatcgccga gcaatccggc attatgcaaa acaccatcca gacgcggata attaacgaca
    18541 atgcgctgtg ccagttgttg gcaattttcg gacgtgcagg tcagcaaatc gagaataaac
    18601 cactgtggct gacgcccagt ttcttcgttt atgtggctgg ctacctgacg taatttttct
    18661 tcattacggc ccaacagaat cactgtcgca ccatagcgtg catacgtcat cgcggcttca
    18721 cgaccaatcc catcgctggc tcccgtcacc aggataatgc gatcattgag taaatcttgt
    18781 tttggctggt aatgcatggc tactcctcaa cgacgttgtc tgtcgcgctg ccagtgtact
    18841 ttatgacttt ggggctttat gcctgaaaca gaagggcatt tcaatcagcc tggggcgata
    18901 acaccgcaaa atggatactt tgtcatactt tcgctgcaat aacatctctg cgagacggct
    18961 taacatgcct gttgtaaact gtgagccaaa gcgttgttta accaaggtgg ggactcgtgg
    19021 aattgttgtc tgaatatggt ttgtttttgg ctaaaatcgt taccgttgtg ctagcgattg
    19081 cggcgattgc cgccattatt gtcaatgttg ctcaacgtaa taaacgccag cgtggcgagt
    19141 tacgggtcaa caatctcagc gaacagtata aggagacgaa agaagaactg gccgcggcgc
    19201 tgatggactc acatcagcaa aaacagtggc acaaagcgca gaagaaaaag cacaagcaag
    19261 aagcgaaagc agcaaaagcg aaagccaaac tgggcgaggt ggcaactgac agtaaacctc
    19321 gcgtctgggt gctggatttt aaaggcagca tggacgccca tgaagtgaac tcgctacgtg
    19381 aagagataac ggcggtactc gcagcattca aaccgcagga tcaggttgtg ctccgtctgg
    19441 aaagccctgg tggcatggtg catggttacg ggttggcggc ttcgcagctg cagcgtctgc
    19501 gtgataaaaa cattccttta actgttacgg tagacaaagt cgctgccagc ggcggttaca
    19561 tgatggcctg tgtggcggac aaaattgttt ccgcaccgtt tgctattgtg ggttccattg
    19621 gggtggtggc gcaaatgccc aactttaacc gcttcctgaa aagcaaagat attgatatcg
    19681 aactgcacac cgccgggcag tataagcgca cgctgacctt gctgggtgaa aataccgaag
    19741 aagggcggga gaaattccgc gaagagctga acgaaacgca tcagttattt aaagattttg
    19801 tgaagcgtat gcgtccgtct ctggatattg aacaggtggc aacgggtgaa cactggtacg
    19861 gacaacaggc ggtagagaaa ggcctggttg atgaaatcaa caccagtgat gaagttattc
    19921 ttagcctgat ggaaggccgt gaagtggtta atgtacgcta tatgcagcgt aaacgactca
    19981 ttgaccgatt caccggcagc gcggcagaga gcgccgatcg attgttgcta cgctggtggc
    20041 agcggggtca aaagccattg atgtaaaaga caaacgcgag gctaagacct cgcgttttgc
    20101 tttaatcaac cagatgatat ttttctgaaa gcacatgggc caggtgtttg aacatattaa
    20161 acaccgcggt gcttttggct gttggcaatc cttgttcatc taaaaagtag tcgccggtaa
    20221 ataccagcac gccattacgc tgcgtgacgc cggtggcttc aatccctgcg agcgtatcct
    20281 catgctcacg aatgattttg ttagcctctt tcaacagcgt ttcgcggtcg atgggttgtg
    20341 tctctttgtt cattatttac tccttaaaca aggacattag tctacgccag gcatggcttg
    20401 cagacaaata taccacgctg gtggcaagag cgccttactg gcaactttgg attttgcatg
    20461 ctaataaagt tgcgtatcgg attttatcag gtacagtgtg acgctttcgt caatctggca
    20521 atagatttgc ttgacattcg accaaaattc cgtcgtgcta tagcgcctgt aggccaagac
    20581 ctgttaactc agtcacctga attttcgtga acagagtcac gacaaggggg tgatatccgc
    20641 agagagcgag tccatatcgg taactcgttg ccagtggaag gtttatcaac gtgcgacgca
    20701 ttcctggaag aatcaaatta ggtaaggtga atatgggtaa agctcttgtc atcgttgagt
    20761 ccccggcaaa agccaaaacg atcaacaagt atctgggtag tgactacgtg gtgaaatcca
    20821 gcgtcggtca catccgcgat ttgccgacca gtggctcagc tgccaaaaag agtgccgact
    20881 ctacctccac caagacggct aaaaagccta aaaaggatga acgtggcgct ctcgtcaacc
    20941 gtatgggggt tgacccgtgg cacaattggg aggcgcacta tgaagtgttg cctagtaaag
    21001 agaaggtcgt ctctgaactg aaacaactgg ctgaaaaagc cgaccacatc tatctcgcaa
    21061 ccgaccttga ccgcgaaggg gaagccattg catggcacct gcgggaagtg attggtggtg
    21121 atgatgcgcg ctatagccga gtggtgttta acgaaattac taaaaacgcg atccgccagg
    21181 catttaacaa accgggtgag ctgaatattg atcgtgttaa tgcccagcag gcgcgtcgct
    21241 ttatggaccg cgtggtgggg tatatggttt cgccgctgct gtggaaaaag atcgctcgtg
    21301 gtctgtctgc cggtcgtgtg cagtcggtgg cggtccgcct ggtggtagag cgtgagcgtg
    21361 aaattaaagc gttcgtaccg gaagagttct gggaagtcga tgccagcacg actacgccat
    21421 ctggtgaagc gttggcgttg caggtgactc atcagaatga caaaccgttc cgtccggtca
    21481 acaaagaaca aactcaggct gcggtaagtc tgctggaaaa agcgcgctac agcgtgctgg
    21541 aacgtgaaga caaaccgaca accagtaaac ctggcgctcc ttttattacc tctacgctgc
    21601 aacaagctgc cagcacccgt cttggttttg gcgtgaaaaa aaccatgatg atggcgcagc
    21661 gtttgtatga agcaggctat atcacttaca tgcgtaccga ctccactaac ctgagtcagg
    21721 acgcggtaaa tatggttcgc ggttatatca gcgataattt tggtaagaaa tatctgccgg
    21781 aaagtccgaa tcagtacgcc agcaaagaaa actcacagga agcgcacgaa gcgattcgtc
    21841 cttctgacgt caatgtgatg gcggaatcgc tgaaggatat ggaagcagat gcgcagaaac
    21901 tgtaccagct aatctggcgt cagttcgttg cctgccagat gaccccagcg aaatatgact
    21961 ccacgacgct gaccgttggt gcgggcgatt tccgcctgaa agcacgcggt cgtattttgc
    22021 gctttgatgg ctggacaaaa gtgatgcctg cactgcgtaa aggcgatgaa gatcgtatct
    22081 taccggcagt cgataaaggc gatgctctga cgctcgttga actaacacca gcccagcact
    22141 ttaccaagcc gccagcccgt ttcagtgaag catcgctggt taaagaactg gaaaaacgtg
    22201 gtatcggtcg tccgtctacc tatgcgtcga tcatttcgac cattcaggat cgtggctatg
    22261 tgcgagtaga aaatcgtcgt ttctatgcgg aaaaaatggg cgaaatcgtc accgatcgcc
    22321 ttgaagaaaa tttccgcgag ttaatgaact acgactttac cgcgcagatg gaaaacagcc
    22381 tcgaccaggt ggcaaatcac gaagcagagt ggaaagctgt actggatcac ttcttctcgg
    22441 atttcaccca gcagttagat aaagctgaaa aagatccgga agagggtggt atgcgcccga
    22501 accagatggt tctgaccagc attgactgcc cgacttgtgg tcgcaaaatg gggattcgca
    22561 cagcgagcac cggggtattc cttggctgtt ctggctatgc gctgccgccg aaagagcgtt
    22621 gcaaaaccac cattaacctg gtgccggaaa acgaagtgct gaacgtgctg gaaggcgaag
    22681 atgctgaaac caacgcgctg cgcgcaaaac gtcgttgccc gaaatgcggc acggcgatgg
    22741 acagctatct cattgatccg aaacgtaagt tgcatgtctg tggtaataac ccaacctgcg
    22801 acggttacga gatcgaagag ggcgaattcc gcattaaagg ttatgacggc ccgatcgttg
    22861 agtgtgaaaa atgtggctct gaaatgcacc tgaaaatggg gcgtttcggt aaatacatgg
    22921 cctgcaccaa cgaagagtgt aaaaacacac gtaagatttt acgtaacggc gaagtggcac
    22981 caccgaaaga agatccggtg ccattacctg agctgccgtg cgaaaaatca gatgcttatt
    23041 tcgtgctgcg tgacggtgct gccggtgtgt tcctggctgc caacactttc ccgaaatcgc
    23101 gtgaaacgcg tgcgccactg gtggaagagc tttatcgctt ccgcgaccgt ctgccggaaa
    23161 aactgcgtta tctggccgat gcgccacagc aggatccgga aggtaataag accatggttc
    23221 gctttagccg taaaaccaaa cagcaatatg tctcttcgga aaaagacgga aaggcgactg
    23281 gctggtcagc attttatgtt gatggcaaat gggttgaagg aaaaaaataa cctttaattc
    23341 tatcaggttt ttataaacaa agggtcgcga aagcggccct tttttattgc atattatttt
    23401 ttcttcacac ctatacacta aggctataaa tgatatagtg gttatagtta gcaccttttt
    23461 tattattaaa tcgtattagt cacccgccag gtgtgacgaa aaaacgatgt tctgatggcg
    23521 tctaagtgga tggtttaaca tgaaattaca acaacttcgc tatattgttg aggtggtcaa
    23581 tcataacctg aatgtctcat caacagcgga aggactttac acatcacaac ccgggatcag
    23641 taaacaagtc agaatgctgg aagacgagct aggcattcaa attttttccc gaagcggcaa
    23701 gcacctgacg caggtaacgc cagcaggaca agaaataatt cgtatcgctc gcgaagtcct
    23761 gtcgaaagtc gatgccataa aatcggttgc cggtgagcac acctggccgg ataaaggctc
    23821 gctgtatatc gccaccacgc atacccaggc acgctacgcg ttaccaaacg ttatcaaagg
    23881 ctttattgag cgttatcccc gcgtttcttt gcatatgcac cagggcccgc cgacacaaat
    23941 tgctgatgcc gtctctaaag gcaatgccga tttcgctatc gccacagaag cgctgcatct
    24001 gtatgaagat ttagtgatgt taccgtgcta ccactggaat cgggctattg tagtcactcc
    24061 ggatcacccg ctggcaggca aaaaagccat taccattgaa gaactggcgc aatatccgtt
    24121 ggtgacatat accttcggct ttaccggacg ttcagaactg gatactgcct ttaaccgcgc
    24181 agggttaacg ccgcgtatcg ttttcacggc aacggatgct gacgtcatta aaacttacgt
    24241 ccggttaggg ctgggggtag gggtcattgc cagcatggcg gtggatccgg tcgccgatcc
    24301 cgaccttgtg cgcgttgatg ctcacgatat cttcagccac agtacaacca aaattggttt
    24361 tcgccgtagt acttttttgc gcagttatat gtatgatttc attcagcgtt ttgcaccgca
    24421 tttaacgcgt gatgtcgttg atgcggctgt cgcattgcgc tccaatgaag aaattgaggt
    24481 catgtttaaa gatataaaac tgccggaaaa ataatttctt gcgttatttt cggcaccttt
    24541 tatgtagcga aggtgccgga atatattctc ttttgttact tatttctata ccgtgcacaa
    24601 tatttttatc cctcatattt atagggtaaa ttacctgtaa agtagtgtct aattacccgc
    24661 ctcgcaaatt ccagttttca aatatctatt tcaggtcaaa agattgaatt attcactgga
    24721 gacgattcgt aaattcgctg gatttttgac taaagtttct ttaggatttg tctcatcgta
    24781 tgattaatta caccaatcat tcaacgtctt gctgataagt gatggcgatt atatgaggtt
    24841 agcaatgcct tctggaaatc aggaaccccg ccgtgatcct gaattaaaac gtaaagcctg
    24901 gctgacggtt tttcttggtt ctgcactttt ctgggtggtt gtcgcactgc tgatttggaa
    24961 agtgtgggga taactatggt cggtcaggag caactggagt cgtcaccatt atgccagcat
    25021 agtgacaatg aaccagaaac gaaacgggaa tgttccgtcg ttattccaga cgactggcaa
    25081 ctaacatcgc agcagcaagc ctttatagaa ctgtttgctg aagatgatca gccgaaacaa
    25141 taattatcat cattcttatt acccattttt aatgaattaa agggctttta atacaccgca
    25201 gcaataacag cttgagttat ctcaacacaa aataataacc gttaagggtg tagcctatga
    25261 tcaacacaaa tatgaaatat tggtcctgga tgggcgcgtt ttctctgtcg atgctcttct
    25321 gggccgaact cctctggatc attactcact gatccttgac cccgctgcgg cggggttgtc
    25381 atttgctttg ccacaaggtt tctcctcttt tatcaatttg ggttgttatc aaatcgttac
    25441 gcgatgtttg tgttatcttt aatatttacc ctgaagagaa tcagggcttc gcaaccctgt
    25501 cattaaggag gagctatgtc gtcaacccta cgagaagcca gtaaggacac gttgcaggcc
    25561 aaagataaaa cttaccacta ctacagcctg ccgcttgctg ctaaatcact gggcgatatc
    25621 acccgtctac ccaagtcact caaagttttg ctcgaaaacc tgctgcgctg gcaggatggt
    25681 aactcagtta ccgaagagga tatccacgca ctggcgggat ggctgaaaaa tgcccatgct
    25741 gaccgtgaaa ttgcctaccg cccggcaagg gtgctgatgc aggactttac cggcgtacct
    25801 gccgttgttg atctggcggc aatgcgcgaa gcggttaaac gcctcggtgg cgatactgca
    25861 aaggttaacc cgctctcacc ggtcgacctg gtcattgacc actcggtgac cgtcgatcgt
    25921 tttggtgatg atgaggcatt tgaagaaaac gtacgcctgg aaatggagcg caaccacgaa
    25981 cgttatgtgt tcctgaaatg gggaaagcaa gcgttcagtc ggtttagcgt cgtgccgcca
    26041 ggcacaggca tttgccatca ggttaacctc gaatatctcg gcaaagcagt gtggagcgaa
    26101 ttgcaggacg gtgaatggat tgcttatccg gatacactcg ttggtactga ctcgcacacc
    26161 actatgatca acggccttgg cgtgctgggg tggggcgtgg gtgggatcga agcagaagcc
    26221 gccatgttag gccagccggt ttccatgctt atcccggacg tagtgggctt taaacttacc
    26281 ggaaaattac gtgaaggtat taccgccaca gacctggttc tcactgttac ccaaatgctg
    26341 cgcaaacatg gcgtggtggg gaaattcgtc gaattttatg gtgatggtct ggattcacta
    26401 ccgttggcgg atcgcgccac cattgccaat atgtcgccag aatatggtgc cacctgtggc
    26461 ttcttcccaa tcgatgcagt aaccctcgat tacatgcgtt taagcgggcg cagcgaagat
    26521 caggtcgagt tggtcgaaaa atatgccaaa gcgcagggca tgtggcgtaa cccgggcgat
    26581 gaaccgattt ttaccagtat gttagaactg gatatgaatg acgttgaagc gagcctggca
    26641 gggccaaaac gcccacagga tcgcgttgca ctgcccgatg taccaaaagc atttgccgcc
    26701 agtaacgaac tggaagtgaa tgccacgcat aaagatcgcc tgccagtcga ttatgttatg
    26761 aacggacatc agtatcagtt acctgatggc gctgtggtca ttgctgcgat aacctcgtgc
    26821 accaacacct ctaacccaag tgtgctgatg gccgcaggct tgctggcgaa aaaagccgta
    26881 actcttggcc tcaagcggca accgtgggtc aaagcgtcgc tggcaccggg ttcgaaagtc
    26941 gtttctgatt atctggcaaa agcgaaactg acaccgtatc tcgacgaact ggggtttaac
    27001 cttgtgggat acggttgtac cacctgtatt ggtaactctg ggccgctgcc cgatcctatc
    27061 gaaacggcaa tcaaaaaagg cgatttaacc gtcggtgcgg tgctgtccgg caaccgtaac
    27121 tttgaaggcc gtatccatcc gctggttaaa actaactggc tggcctcgcc gccgctggtg
    27181 gttgcctatg cgctggcggg aaatatgaat atcaacctgg cttctgagcc tatcggccat
    27241 gatcgcaaag gcgatccggt ttatctgaaa gatatctggc catcggcaca agaaattgcc
    27301 cgtgcggtag aacaagtctc cacagaaatg ttccgcaaag agtacgcaga agtttttgaa
    27361 ggcacagcag agtggaagga aattaacgtc acacgatccg atacctacgg ttggcaggag
    27421 gactcaacct atattcgctt atcgcctttc tttgatgaaa tgcaggcaac accagcacca
    27481 gtggaagata ttcacggtgc gcggatcctc gcaatgctgg gggattcagt caccactgac
    27541 catatctctc cggcgggcag tattaagccc gacagcccag cgggtcgata tctacaaggt
    27601 cggggtgttg agcgaaaaga ctttaactcc tacggttcgc ggcgtggtaa ccatgaagtg
    27661 atgatgcgcg gcaccttcgc caatattcgc atccgtaatg aaatggtgcc tggcgttgaa
    27721 ggggggatga cgcggcattt acctgacagc gacgtagtct ctatttatga tgctgcgatg
    27781 cgctataagc aggagcaaac gccgctggcg gtgattgccg ggaaagagta tggatcaggc
    27841 tcaagccgtg actgggcggc aaaaggacct cgtctgcttg gaattcgtgt ggtgattgcc
    27901 gaatcgtttg aacgaattca ccgttcgaat ttaattggca tgggcatcct gccgctggaa
    27961 tttccgcaag gcgtgacgcg taaaacgtta gggctaaccg gggaagagaa gattgatatt
    28021 ggcgatctgc aaaatctaca acctggcgcg acggttccgg tgacgcttac gcgcgcggat
    28081 ggtagccagg aagtcgtacc ctgccgttgt cgtatcgaca ccgcgacgga gttgacctac
    28141 taccagaacg acggcatttt gcattatgtc atccgtaata tgttgaagta acaagtattt
    28201 gcttgccggt tattttgctt ccggcaagca aatgaattac acaatgcaag agggttattt
    28261 gttcagcaaa tggcccattt tctcggcttt ggtatcgaga taatgttcgt tattggggtt
    28321 acgacctaca atcaatggta cgcgttcaac aatattaatc cctgcttcgg tcagaatttc
    28381 gacttttttc gggttattgg ttaacaagcg gacttcattg acgccaagga gtttgaacat
    28441 atcagcgcaa agagtgaagt cgcgctcatc agcggcgaag cctaactggt ggttagcctc
    28501 tacggtatcg taaccttgat cctgcagtgc gtaagcgcgg attttattca gcagaccaat
    28561 gttacgacct tcctgacggt gatacagcaa aataccacgg ccttcctcgg caatttgcgt
    28621 caatgccgct tcgagctgga agccacaatc gcagcgcaag ctgaacaggg cgtcaccggt
    28681 cagacattcg gaatggacgc gcgcaagtac cggggtatgc ccggaaatat cggcatagac
    28741 tagcgcgaca tgatcgtgtc cggttgccag ttcttcaaat cccaccatca ggaaatcgcc
    28801 ccatggggtt ggcagtttgg cttctgccac acgtttaagc tgcatgaaat tctccagata
    28861 atgctggttc tgtattggct tattttgcca taacgagaag gggttcacct aatcacgacg
    28921 cgtcgatcgt tcacggaatg gcacaaatct gtcaattttt cttggaactg gcattttcag
    28981 ttatgattgt gggacttatc aaaaaggaga ggccatgcgt tcgattgccc gacgtaccgc
    29041 agtgggagct gcactattgt ttgtcatgcc agtagccgta tggatttctg gctggcgttg
    29101 gcaacctgga gaacaaagtt ggcgactaaa agcggctttt tgggttactg aaactgtcac
    29161 ccagccctgg ggcgtcatta cacatttgat tttgttcggc tggtttctct ggtgtctgcg
    29221 ttttcgcatt aaggctgcct ttgtattatt ttccattctg gcggcggcaa tccttgttgg
    29281 acaaggcgtt aaatcctgga tcaaagacaa agtacaggaa ccacgacctt ttgttatctg
    29341 gttggaaaaa acacatcata ttccggttga tgagttctac actttaaagc gagcagaacg
    29401 cggaaatcta gtaaaagaac agttggctga agagaaaaat attccacaat atttgcgttc
    29461 acactggcag aaagagacgg ggtttgcctt tccttccggt cacacgatgt ttgctgccag
    29521 ttgggcactg ctggccgttg gtttgctgtg gccgcgtcgg cgaacgttaa ccattgctat
    29581 cttgttggtc tgggcaacgg gagtcatggg aagccgcctg ctactcggga tgcattggcc
    29641 acgcgatctg gtggtagcta cgttgatttc gtggacgctg gtggcggtgg caacgtggct
    29701 tgcgcaacga atttgtgggc cattaacacc acctgcggaa gaaaatcgcg aaattgcaca
    29761 acgagaacaa gaaagttaac gctggttgat tttccgaatt taggccttaa atcatcaaca
    29821 atgcgcgtgg atgccatttc gcagacggcg cgaaaatggt actttaaagg gctattgcgg
    29881 taagttgacc ataatttatt cgctctaacc acataacggg aagtaatgtg aaatatttac
    29941 tcattttctt actggtgtta gcgatcttcg tgatttcggt cacgttgggt gcgcagaacg
    30001 atcaacaggt gacgtttaat tatctgttag cgcaagggga gtaccgtatt tccacattgc
    30061 tggcggtatt gtttgctgcg gggtttgcta tcggttggtt gatttgtggc ctgttctggc
    30121 tgcgagttcg tgtttccctg gcgcgcgctg aacgtaaaat aaagcgactg gaaaaccagc
    30181 tttcacccgc gactgacgtg gctgtagtgc cgcactcgtc agcggcgaag gaataacttt
    30241 ctatgctgga gttgttgttt ctgcttttgc ctgtagccgc tgcctatggc tggtatatgg
    30301 gccgcagaag tgcgcaacaa aacaagcaag atgaagccaa ccgcttgtcg cgtgattacg
    30361 tagcgggggt taacttcctg cttagtaatc aacaggataa agcggtagac ctgtttctcg
    30421 atatgcttaa agaggataca ggcaccgttg aagcccacct tacgctcgga aacctgttcc
    30481 gttcgcgtgg cgaagttgat cgcgctattc gcatccatca gaccctaatg gaaagcgcct
    30541 cgctgaccta tgaacagcgt ctgttggcga ttcaacaact ggggcgtgat tacatggccg
    30601 ccgggttata cgaccgcgcg gaagacatgt tcaatcagct gaccgatgaa actgacttcc
    30661 gcattggcgc gctgcaacag ttgctacaaa tctaccaggc taccagtgag tggcagaaag
    30721 caattgatgt tgccgaacgc ctggtgaagc tgggtaaaga taaacagcgc gtcgaaattg
    30781 cccatttcta ctgtgagtta gccctgcagc atatggccag cgacgatctc gatcgtgcca
    30841 tgaccttgct aaaaaaaggg gcggcggcag ataaaaacag cgcccgcgta tccatcatga
    30901 tgggacgcgt gtttatggcg aaaggagaat acgccaaagc cgtcgaaagt ttgcaacgcg
    30961 tcatatccca ggacagagaa ctggtcagcg aaacgctgga aatgttgcaa acctgctatc
    31021 agcagttggg taaaactgcc gaatgggcag aattcctgca acgcgcggtg gaagagaaca
    31081 ccggtgccga tgctgaattg atgcttgctg atatcatcga agcgcgcgac ggtagtgagg
    31141 ctgcacaggt ctatattaca cgccagcttc agcgtcatcc gaccatgcgt gtattccata
    31201 agctaatgga ttaccactta aatgaagcgg aagaagggcg cgccaaagag agtctgatgg
    31261 tgctgcgtga catggttggc gagaaggtgc gtagtaagcc tcgttatagc tgccagaaat
    31321 gtggttttac cgcatacacc ctctactggc attgtccgtc ttgtcgtgcc tggtcaacta
    31381 ttaaaccgat tcgcggtctt gatggcctgt aatttttaaa aaaatccgac tttagttaca
    31441 acatactagt tattgatgtc ccattgtgct ccggcaacga cggagcagaa aaagcctgct
    31501 aggggaacaa tcgcaactgt taatttttta tttccacggg tagaatgctc gccgtttacc
    31561 tgtttcgcgc cacttccggt gcccatcatc aagaaggtct ggtcatgacg ttaactgctt
    31621 catcttcttc ccgcgctgtt acgaattctc ctgtggttgt tgcccttgat tatcataatt
    31681 gtgatgacgc gctgtccttt gtcgacaaga tcgacccacg cgattgtcgt ctgaaggtcg
    31741 gtaaagagat gtttacattg tttgggccac agtttgtgcg cgaacttcaa cagcgtggtt
    31801 ttgatatctt tcttgacctg aaattccacg atatccccaa cactgcagcg cacgctgtcg
    31861 ctgctgcagc tgacttaggc gtgtggatgg tgaatgctca tgcctccggt ggggcgcgta
    31921 tgatgaccgc agcgcgtgag gcactggttc cgtttggcaa agatgcaccg cttttgattg
    31981 ctgtgacagt gttgaccagc atggaagcca gcgacctggc cgatcttggc gtgacactgt
    32041 cacctgcaga ttatgcagaa cgtctggcag cactgacgca aaaatgtggc cttgatggtg
    32101 tggtgtgttc tgctcaggaa gctgtgcgct ttaaacaggt attcggtcag gagttcaaac
    32161 tggttacacc gggcatccgt ccgcagggga gtgaagctgg tgaccagcgc cgcattatga
    32221 cgccagaaca ggcgttggcg gctggtgttg attatatggt gattggtcgc ccggtaacgc
    32281 aatcggtaga tccagcgcag acactgaaag cgatcaacgc ctctttacag cggagtgcat
    32341 gatgagtgat tccaacagcc gtctggtcta ctcaacggaa accgggcgta ttgatgagcc
    32401 caaagcggcc ccagtacgcc ctaaaggcga tggagcggtg cgtattcagc gtcagaccag
    32461 tggacgtaaa ggtaagggcg tttgcctgat taccggtatc gatctcgatg atgccgaact
    32521 gacaaaactt gcagcggaac tgaagaaaaa atgcggctgc ggcggagcag ttaaagatgg
    32581 agttattgaa atccagggcg ataagcgtga tttattaaaa tcactacttg aagcgaaagg
    32641 gatgaaggta aaactcgcag gcggttaacg taaaaagcca cggatatatc cgtggctttc
    32701 gaatatttta ctgtgtgtat tattcagtta tttcgtactg aacagaatca agcgtattat
    32761 aattgcgagt cgataatcga catttattta cgattattta ccgacctggt gactaataac
    32821 accaccgaca gctgcaccac ctaatgtacc caacgtactg ccatcggtca gtactgcacc
    32881 gcctaatgcc cctgcacccg cgccgattgc ggtgttgcgg tcccgtttag accagttaga
    32941 acaggcactc agagacattg ccaaagtaat tgccagaaca gccgcggtca tttttttgct
    33001 cgttacaaac ataatactct ctcctgaatt tatgattcac ggaagtaagc tctctataac
    33061 tataatagct aagaataagc ctggtgaaat tattccgtga aatctgctcg cgcgcagggt
    33121 tatatcacg
//
LOCUS       DAJTYF010000031        33005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTYF010000031 DAJTYF010000000
VERSION     DAJTYF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.031"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            17..4549
                     /locus_tag="OO590_001619"
     CDS             17..4549
                     /locus_tag="OO590_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCR6637023.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDVWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSS
                     SDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTP
                     DDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIA
                     DALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDKTANGGAGAYNAS
                     HDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEEN
                     GAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALG
                     SSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEA
                     HDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIG
                     LNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEF
                     SVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVT
                     NIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAE
                     EENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNIT
                     LGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQRMVEMDNKLSKTESKL
                     SGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGST
                     NSQGEYSAALGAGIQW"
     gene            4917..6572
                     /gene="lldP"
                     /locus_tag="OO590_001620"
     CDS             4917..6572
                     /gene="lldP"
                     /locus_tag="OO590_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6637024.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6572..7348
                     /gene="lldR"
                     /locus_tag="OO590_001621"
     CDS             6572..7348
                     /gene="lldR"
                     /locus_tag="OO590_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6637025.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7345..8535
                     /gene="lldD"
                     /locus_tag="OO590_001622"
     CDS             7345..8535
                     /gene="lldD"
                     /locus_tag="OO590_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6637026.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8731..9204
                     /gene="trmL"
                     /locus_tag="OO590_001623"
     CDS             8731..9204
                     /gene="trmL"
                     /locus_tag="OO590_001623"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6637027.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9257..10078)
                     /gene="cysE"
                     /locus_tag="OO590_001624"
     CDS             complement(9257..10078)
                     /gene="cysE"
                     /locus_tag="OO590_001624"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6637028.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10158..11177)
                     /gene="gpsA"
                     /locus_tag="OO590_001625"
     CDS             complement(10158..11177)
                     /gene="gpsA"
                     /locus_tag="OO590_001625"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6637029.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11177..11644)
                     /gene="secB"
                     /locus_tag="OO590_001626"
     CDS             complement(11177..11644)
                     /gene="secB"
                     /locus_tag="OO590_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6637030.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11707..11958)
                     /gene="grxC"
                     /locus_tag="OO590_001627"
     CDS             complement(11707..11958)
                     /gene="grxC"
                     /locus_tag="OO590_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6637031.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12100..12531)
                     /locus_tag="OO590_001628"
     CDS             complement(12100..12531)
                     /locus_tag="OO590_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6637032.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12776..14320
                     /gene="gpmM"
                     /locus_tag="OO590_001629"
                     /gene_synonym="pgmI"
     CDS             12776..14320
                     /gene="gpmM"
                     /locus_tag="OO590_001629"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6637033.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14330..15613
                     /gene="envC"
                     /locus_tag="OO590_001630"
     CDS             14330..15613
                     /gene="envC"
                     /locus_tag="OO590_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6637034.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15617..16576
                     /locus_tag="OO590_001631"
     CDS             15617..16576
                     /locus_tag="OO590_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6637035.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16563..17597)
                     /gene="waaH"
                     /locus_tag="OO590_001632"
     CDS             complement(16563..17597)
                     /gene="waaH"
                     /locus_tag="OO590_001632"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR6637036.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17836..18861)
                     /gene="tdh"
                     /locus_tag="OO590_001633"
     CDS             complement(17836..18861)
                     /gene="tdh"
                     /locus_tag="OO590_001633"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6637037.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18871..20067)
                     /gene="kbl"
                     /locus_tag="OO590_001634"
     CDS             complement(18871..20067)
                     /gene="kbl"
                     /locus_tag="OO590_001634"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6637038.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20342..21199)
                     /gene="yibB"
                     /locus_tag="OO590_001635"
     CDS             complement(20342..21199)
                     /gene="yibB"
                     /locus_tag="OO590_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR6637039.1"
                     /translation="MKSSTTIITAYFDIGRGDWTTNKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKSYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21503..22435
                     /gene="rfaD"
                     /locus_tag="OO590_001636"
     CDS             21503..22435
                     /gene="rfaD"
                     /locus_tag="OO590_001636"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6637040.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22445..23491
                     /gene="rfaF"
                     /locus_tag="OO590_001637"
     CDS             22445..23491
                     /gene="rfaF"
                     /locus_tag="OO590_001637"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6637041.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23495..24487
                     /gene="rfaC"
                     /locus_tag="OO590_001638"
     CDS             23495..24487
                     /gene="rfaC"
                     /locus_tag="OO590_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6637042.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24484..25692
                     /gene="rfaL"
                     /locus_tag="OO590_001639"
     CDS             24484..25692
                     /gene="rfaL"
                     /locus_tag="OO590_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR6637043.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25728..26870)
                     /locus_tag="OO590_001640"
     CDS             complement(25728..26870)
                     /locus_tag="OO590_001640"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR6637044.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26879..27892)
                     /locus_tag="OO590_001641"
     CDS             complement(26879..27892)
                     /locus_tag="OO590_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR6637045.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(27917..28624)
                     /gene="rfaY"
                     /locus_tag="OO590_001642"
     CDS             complement(27917..28624)
                     /gene="rfaY"
                     /locus_tag="OO590_001642"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6637046.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(28650..29657)
                     /gene="waaO"
                     /locus_tag="OO590_001643"
                     /gene_synonym="rfaI"
     CDS             complement(28650..29657)
                     /gene="waaO"
                     /locus_tag="OO590_001643"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6637047.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(29700..30497)
                     /gene="rfaP"
                     /locus_tag="OO590_001644"
     CDS             complement(29700..30497)
                     /gene="rfaP"
                     /locus_tag="OO590_001644"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6637048.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(30490..31614)
                     /locus_tag="OO590_001645"
     CDS             complement(30490..31614)
                     /locus_tag="OO590_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6637049.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(31611..32669)
                     /gene="rfaQ"
                     /locus_tag="OO590_001646"
     CDS             complement(31611..32669)
                     /gene="rfaQ"
                     /locus_tag="OO590_001646"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6637050.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         8731 a         8070 c         7788 g         8416 t
ORIGIN      
        1 atagcgagca tcattaatga acaaaatatt taaagttatc tggaaccctg cgacagggaa
       61 ttatactgtt accagcgaaa cggcaaaaag ccgtggcaag aaatctgggc gcagtaagct
      121 gttaatttct gcgctggttg cgggtggaat gttgtcgtcg tttggggcat tggcgaatgc
      181 cgggaatgac aacggtcagg gtgttgatta cggtagtgga tcagctggcg acgtctgggt
      241 tgctataggc aaaggggcga aagcaaatac ttttatgaac accagtggtt ccagtactgc
      301 tgtgggttat gacgctatag ctgaaggcca atatagctct gccatcgggt caaaaaccca
      361 tgcgattggt ggtgcatcaa tggcctttgg ggttagtgca atatcagaag gcgatagaag
      421 tatagcactg ggtgcctctt cgtattcatt gggccaatac tcaatggccc tggggcgtag
      481 cagtgtagct agcggtactg acagcctcgc atttggcaga caatcacttg ccagcgcagc
      541 gaacgctatt gcgataggtg ctgagaccga agccgctgaa aatgcaactg ctattggcaa
      601 taatgcgaag gcaaaaggga ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa
      661 agtcaatact atcgcattag gaaatggcag ccaggctctg gcagataatg caatcgccat
      721 aggccagggc aacaaagctg atggcgtgga tgccatcgct ctgggtaatg gtagccagtc
      781 gagaggctta aacaccattg ccttaggcac agccagtaat gcaactggtg ataagagtct
      841 tgcgcttggt agtaatagca gtgccaacgg tattaactct gtcgcgctgg gcgcagattc
      901 cattgcggat ttagacaata ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt
      961 taatgtgaaa aatggcgcga tcaagtctga cagttacgat gccattaatg gttcacagct
     1021 ttatgccatt agcgactcgg tagcaaaaag gcttggagga ggggctgcag tagatgttga
     1081 tgacggtact gttacagcac caacctacaa tttaaaaaat ggtagcaaaa ataacgtagg
     1141 ggctgcgctc gctgtacttg atgaaaacac cctgcaatgg gaccaaacca aaggcaaata
     1201 cagcgctgct catggtacta gtagcccaac tgccagcgta atcaccgatg ttgcggatgg
     1261 cacgatttca gcctccagta aggatgcggt taacggttcc caactgaaag ctaccaatga
     1321 cgatgtcgaa gccaacacca ccaatatcac caacctgacg gattccgttg gtgaccttca
     1381 ggctgatgcc ctgctctgga acgaaactaa aaaggcattc agtgcagctc acggccagga
     1441 taccaccagc aaaatcacca acgttaaaga tgccgacctg acggctgaca gcactgatgc
     1501 tgttaacggc tctcagctga aaaccaccaa cgatgctgtg gcgacgaata ccaccaatat
     1561 cgccaataac acttccaata ttgccactaa caccaccaac atctctaacc tgactgagac
     1621 ggtgactaat cttggtgagg atgcgctgaa atgggataag gacaatggtg tattcacggc
     1681 agctcatggc accgagacca ccagcaaaat aaccaacgtt aaagatggcg acctgacgac
     1741 tggcagcacc gatgccgtta acggctctca gctgaaaacc accaacgatg ccgtggcgac
     1801 gaataccacc aatatcgcca ctaacaccac caacatctct aatctgactg agacggtgac
     1861 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtcttca ctgcagctca
     1921 tggcaacaat accgccagca aaatcaccaa tatcctggac ggcacagtca ctgcaaccag
     1981 ttccgatgcc attaacggta gccagcttta tgacttaagc agcaatatcg ccacctactt
     2041 cggcggcaat gcttctgtga atactgacgg cgtgtttacc ggtccaacct acaaaatcgg
     2101 tgaaacaaat tattataacg tcggcgatgc tctgctttgg gatgccaccg caggtaaatt
     2161 cagtgccaaa cacggtacta atggtgacgc aagcgtgatc actgatgtcg cagatggtga
     2221 aatttcagac tccagttctg acgcagtaaa cggctcacaa ctccacggcg tgagcagtta
     2281 tgttgttgat gcgctggggg gtggtgccga agtcaatgca gacggcacca tcactgcgcc
     2341 gacgtacacc attgctaatg ctgattacga taatgtcggt gatgccctga atgctatcga
     2401 taccactcct gacgacgctc tgctctggga tgcggacgcc ggtgaaaatg gtgcatttag
     2461 cgccgctcac ggaaaagata aaactgccag tgtaatcact aacgtcgcta acggtgcaat
     2521 ctctgctgcc agcagcgacg cgattaacgg ctcacaactc tataccacca ataagtacat
     2581 cgctgatgcg ctgggtggtg acgcagaagt caacgctgac ggcaccatca ccgcaccgac
     2641 ttacaccatt gcgaacgccg agtacaacaa cgtcggtgac gccctggatg cgcttgatga
     2701 taacgccctg ctgtgggata agactgccaa tggcggtgct ggagcctaca atgccagcca
     2761 tgacggtaaa gccagcatca tcactaatgt cgctaatggc agtattagtg aggacagtac
     2821 cgatgcagtg aacggttctc agttgaatgc gacgaatatg atgattgagc agaacaccca
     2881 aattatcaat cagctcgctg gtaacaccga cgcaacctat atcgaagaaa atggcgctgg
     2941 tatcaactac gttcgtacta acgacaacga tttagccttt aacgatgcaa gcgcctctgg
     3001 tgtcggcgct acagctgtag gttataacgc tgtcgcgtct ggtgccagca gcgtagccat
     3061 tggtcagaac agcagcagca ccgttgatac cggtattgcg ctgggtagca gctccgtttc
     3121 cagccgtgtg attgccaaag gttctcgtga cactagcgta acggaaaatg gcttggtgat
     3181 tggttatgac accactgacg gcgaactgct aggtgcattg tcaattggtg atgacggtaa
     3241 ataccgccaa atcatcaacg tagctgatgg ttcagaagcc catgacgccg ttacggttcg
     3301 ccagttgcag aacgctattg gagcggtcgc cactacgcca accaagtact ttcacgccaa
     3361 ctcaacggca gaagactcac tggccgttgg tgaagactcg ctggcaatgg gtgcgaaaac
     3421 tgtcgttaat ggtaatgcag gtattggtat cggtttgaac actctggttc tggctgatgc
     3481 gatcaacggc attgctatcg gcagtaacgc acgcgcaaac catgcaaaca gtatcgcaat
     3541 gggtaacggt tctcagacca cccgtggtgc acagactggc tacaccgcct acaacatgga
     3601 cgcaccgcag aactctgtag gtgagttttc tgtcggtagt gaagacggtc aacgtcagat
     3661 cacaaacgtc gcagctggtt cagcggatac cgatgcggtt aacgtgggtc agttgaaagt
     3721 cactgatgag cgcgtagcgc aaaataccca gagcattact aacctgaaca atcaggtcac
     3781 taatctggat actcgcgtta ctaatatcga aaacggtatt ggcgacattg tcaccaccgg
     3841 tagcaccaag tacttcaaga ccaacaccga tggcgtagat gccaacgccc agggtaaaga
     3901 tagcgttgct attggttctg gttccattgc tgccgctgac aacagcgtcg cactgggtac
     3961 cggttccgtt gcagaggaag aaaatacaat ctctgtaggt tcttctacta accaacgccg
     4021 tatcaccaac gtagctgcca gtgttaatgc caccgatgcg gttaacgtgt cgcagctgaa
     4081 atcttctgaa gcaggcggag tgcgctacga caccaaagct gatggttcta tcgactatag
     4141 caatatcacc ctcggtggtg gcaacggcag tacgactcgt atcagcaacg tctccgctgg
     4201 cgtcaacaac aacgacgcgg tgaactacgc gcagttgaag caaagcgcgc aagaaacgaa
     4261 gcaatacacc gatcagcgga tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa
     4321 gttgagcggt ggtatcgctt ctgcaatggc aatgaccggt ctgccgcagg cttatacacc
     4381 gggtgccagc atggcttcta ttggtggcgg tacttacaac ggtgaatcgg cagttgcttt
     4441 aggtgtatcg atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa
     4501 tagccagggt gaatactccg ccgcactcgg tgccggtatt cagtggtaat gatccattaa
     4561 gttagtgtga ctaagggcaa acgccaaatt gcctgatgcg ttacgcgcat caggcaagat
     4621 aaggcgctcc gggtcaacaa tctaagggca attctctgat gaggattgcc cttttcttta
     4681 ccagacatct ccccccacaa gaattggccc taccaattct tcgcttatct gacctctggt
     4741 tcacaattcc ccaattaaaa ctcacatcaa tattgataat acataacatt tagttaacca
     4801 ttcattgtca ttatccctac acaacacaat tggcagtgcc acttttacac aacgtgtgac
     4861 aaggagatga gcaacagact cattacacga tgtgcgtgga ctctaggaga cctgcaatga
     4921 atctctggca acaaaactac gatcccgccg ggaatatctg gctttccagt ctgatagcat
     4981 cgcttcccat cctgtttttc ttctttgcgc tgattaagct caaactgaaa ggatacgtcg
     5041 ccgcctcatg gacggtagca atcgcccttg ccgtggcttt gctgttctat aaaatgccgg
     5101 tcgctaacgc gctggcctcg gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt
     5161 ggatcattat tgcagcagtg ttcgtctata agatctcggt gaaaaccggg cagtttgaca
     5221 tcattcgctc gtctattctt tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg
     5281 gtttctgttt cggcgctttc cttgaaggag ccgcaggctt tggcgcaccg gtagcaatta
     5341 ccgccgcatt gctggtcggc ctgggtttta aaccgctgta cgccgccgga ctgtgcctga
     5401 ttgttaacac cgcgccagtg gcatttggcg cgatgggcat tccaatcctg gttgccggac
     5461 aagtaacagg catcgacagc tttgagattg gccagatggt ggggcgtcag ctaccgttta
     5521 tgaccattat cgtgctgttc tggatcatgg cgattatgga cggctggcgc ggtatcaaag
     5581 agacgtggcc tgcggtcgtg gttgcaggcg gctcgtttgc catcgctcag taccttagct
     5641 ctaacttcat tgggccggag ctgccggaca ttatctcttc gctggtatca ctgctctgcc
     5701 tgacgctgtt cctcaaacgc tggcaaccag ttcgcgtatt ccgcttcggt gacttggggg
     5761 cgtcacaggt tgatataacg ctggcccaca ccggttacac cgcgggtcag gtgttacgtg
     5821 cctggacacc gttcctgttc ctgaccgcta ccgtaacact gtggagcatc ccgccgttta
     5881 aagccctgtt cgcatcgggt ggtgcgctgt atgagtgggt gatcaacatt ccggtgccgt
     5941 acctcaataa actggttgcc cgtatgccac cagtggtcag cgaggctaca gcctatgccg
     6001 ccgtgtttaa gtttgactgg ttctctgcca ccggcaccgc cattctgttt gctgcactgc
     6061 tctcgattgt ctggctgaag atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc
     6121 tgaaagaact agctctaccc atctactcca tcggcatggt gctggcgttc gcctttatct
     6181 cgaactattc cggactgtca tcaacactgg cactggcact ggcgcacacc ggtcatgcat
     6241 tcaccttctt ctcgccgttc ctcggctggc tgggggtatt cctgaccggg tcggatacct
     6301 catctaacgc cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg
     6361 atctgttgct ggttgccgcc aataccaccg gtggcgtcac cggtaagatg atctccccgc
     6421 aatctatcgc tatcgcctgt gcggcggtag gcctggtggg caaagagtct gatttgttcc
     6481 gctttactgt caaacacagc ctgatcttca cctgtatggt gggcgtgatc accacgcttc
     6541 aggcttatgt cttaacgtgg atgattcctt aatgattgtt ttacccagac gcctgtcaga
     6601 cgaggttgcc gatcgtgtgc gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa
     6661 gttgcccgct gagcgccaac tggcgatgca actcggcgta tcacgtaatt cactacgcga
     6721 ggcgctggca aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt
     6781 tattcgctgg cgtcatgaca catggtcgga gcaaaacatc gtccagccgc taaaaacgct
     6841 gatggccgat gatccggatt acagtttcga tattctggaa gcccgctacg ccattgaagc
     6901 cagcaccgca tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa agattcagct
     6961 ttgctttgaa gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt
     7021 tcatctggcg attgccgaag cctcacataa catcgtgctg ctgcaaacca tgcgcggttt
     7081 cttcgatgtc ctgcaatcct cagtgaagca tagccgtcag cggatgtatc tggtgccacc
     7141 ggttttttca caactgaccg aacaacatca ggctgtcatt gacgccattt ttgctggtga
     7201 tgccgacggg gcgcgtaaag caatgatggc gcaccttagt tttgttcaca ccaccatgaa
     7261 acgattcgat gaagatcagg ctcgccacgc acggattacc cgcctgcccg gtgagcataa
     7321 tgagcattcg agggagaaaa acgcatgatt atttccgcag ccagcgatta tcgcgccgca
     7381 gcgcaacgca ttctgccgcc gttcctgttc cactatatgg atggtggtgc atattctgaa
     7441 tacacgctgc gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca gcgtattctg
     7501 aaaaacatgt ccgacttaag cctggaaacg acgctgttta atgagaaatt gtcgatgccg
     7561 gtagcactgg caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca
     7621 gccaaagcgg cggacgcgca tggcattccg tttactctct cgacggtttc cgtttgcccg
     7681 attgaagaag tcgcgccagc catcaagcgc ccaatgtggt tccagcttta tgtactgcgc
     7741 gatcgcggct ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg
     7801 gttttcaccg tggatatgcc gacaccgggc gcacgctacc gtgatgcgca ttcaggtatg
     7861 agcggcccaa atgcggcaat gcgccgctac ttgcaggcgg tgacgcatcc gcaatgggcg
     7921 tgggatgtgg gcctgaacgg tcgtccacat gatttaggta atatctcagc ttatctcggc
     7981 aaaccgaccg gactggaaga ttacatcggc tggctgggga ataacttcga tccgtccatc
     8041 tcatggaaag accttgagtg gatccgcgat ttctgggatg gcccgatggt gatcaaaggg
     8101 atcctcgatc cggaagatgc gcgcgatgca gtacgttttg gtgctgatgg aattgtggtt
     8161 tctaaccacg gtggccgcca gctggacggt gtactctctt ccgctcgtgc gttgcccgct
     8221 attgcagatg cggtgaaagg tgatatcgcc attctggcgg atagcggaat tcgtaacggg
     8281 cttgatgtcg tgcgtatgat tgcgctcggt gccgacaccg tactgctggg tcgtgctttc
     8341 ctgtatgcgc tggcaacagc gggccaggcg ggtgtagcta acctgctaaa tctgatcgaa
     8401 aaagagatga aagtggcgat gacgctgact ggcgcgaaat cgatcagtga aattacgcaa
     8461 gattcgctgg tgcaggggct gggtaaagag ttgcctacgg cactggctcc gatggcgaaa
     8521 gggaatgcgg catagtcgtt tgcgccccct caccctaacc ctctccctca gggagagggg
     8581 accgttcggc gctgtatgta ctccctcact ctgaaacgac accgcactct ttttttctcc
     8641 ctcgcccctc cggggagggc cggggtgagg ggaaaaggcc gcacggttca ccaattttct
     8701 gctatcctgc ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa
     8761 attccgccaa atactggcaa catcatccgt ctttgcgcta ataccggctt tcgtctacat
     8821 atcatcgaac cgatgggatt tgcctgggac gacaagcgcc tgcgccgcgc ggggctggac
     8881 tatcacgagt ttaccgccgt tacgcgtcat catgactatc gcgcgttcct cgaagcagaa
     8941 aatccccagc gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaagc
     9001 tatcaggatg gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt
     9061 cttgatgccc tgcccgctga acaaaaaatt cgcattccga tggtgccgga cagccgcagc
     9121 atgaatctgt ccaatgcggt gtcggtagtg gtgtatgaag cctggcggca gctgggatat
     9181 ccgggagcgt tgttgagaga ttagttactg tatgccggat agatgattac atcgcatccg
     9241 gcacgatcac aggacattag atcccatccc catactcaaa tgtatggtta ataccgttga
     9301 aatgctggtc catatccatt gatggcttat cgctgtctgg tttaccgaca atacgagccg
     9361 gaacgccagc ggcggttgta tgcggcggca ccggttgcag caccacggaa cctgcgccaa
     9421 tcttcgcgcc gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg ccaatcatca
     9481 caccttcacg aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca
     9541 cagattgcag aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca acgatgcccg
     9601 tggcgtggtc gagcataatg ccacggccaa tttttgctgc cgggtgaata tcgacctgga
     9661 acgtcacaga aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc tgattccaca
     9721 accagtgacc gatgcgatag gcctgcaagg catgaaaacc cttcaggtat aacaacgggg
     9781 ttgagtattt atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca caggccgcag
     9841 aggcgatcat ttccgggtca gcggcgtagg cttcttcaac cacttcacgg atagcaatag
     9901 caggcataat tggcgatgac agcttgttcg ccagcatgta gctcagtgca ctgccaaggt
     9961 tttcgtgctt gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca
    10021 gcgttctggc ttcggcttta atattgttcc agacaatttc cagttcttca cacgacattg
    10081 cttactccac acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagcggta
    10141 acaaaggttc cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg acctaataat
    10201 gtcaatgctg cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt
    10261 attggcattt caacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag
    10321 ccttccacca cctgaccaat cttctcctgc gcgctttgta catccatgcc ctgaccgagc
    10381 atcatgccaa aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc
    10441 agccccgcca tgcccataaa ggtggcagga tcggcaccca gcgccgcacc aagacgcgac
    10501 atttcagcca gcccacgggt gatcagcgcc gtacgcgcat tcgcaccaaa accgataccg
    10561 tcagacatcc ccgcgccaat ggcaatgacg tttttcaccg cgccgccaag ctgcacgcca
    10621 atgaaatccg ggttgctgta aacgcggaaa cttttgccgc agtgcagcag ctgctggaga
    10681 tcatcggcaa aggtctgatc ggtcgaggcc agcgaaatag ctgtcggtaa acctgccgcc
    10741 agttctttcg caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc
    10801 tcacgcgcca cgtcctgtaa cagacgtccg gtttccgctt ccagcccttt ggtcgcccac
    10861 accagacgcg catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca
    10921 tggctgggta cgacgacgag aatattacga ctggctgcca gcgcagtggc gagatcgctt
    10981 tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg
    11041 cgttcaagcg ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt
    11101 cttgccaggg taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa
    11161 gcattacgtt ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct
    11221 gcaaatagtt catgaacagc gcatcgaagt taaccggcgc aaggttcagt tgcgggaatg
    11281 taccgcggga taccatgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc
    11341 agtatgcgcc caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac
    11401 cgccctgctg aacttcacac aggaacgcgg tttcttcgcc caaggaggcc gttacggtaa
    11461 cacgcagtac cacttcgtat acgtcatctg ccagttggga agatgccgta tccagatcaa
    11521 gtttaacttc tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag
    11581 agatatcctt ggtataaata cgttggatct ggaaagtcat ttcagtgttg ttttgttctg
    11641 acatgtgtag aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt
    11701 cacacgttat ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc
    11761 acagccgcca atgtgctgtg cgtcaataaa aatctgggga accgtggtgc gaccgctgcg
    11821 tttgatcatc tcttcacgct tggcggcatt gccatcgatc ggcaactcct ggaaactcac
    11881 gcccttgctg ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata
    11941 gatttcaaca ttggccataa tttagctcct gtttttttac cctgaaattt catgttgcag
    12001 ggcggctgca aaatggagtg tccccagaag cttataacaa taagtcgctg ggggcctaat
    12061 tgcagctaac ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccagagg
    12121 caagttttcg ccagcccagc cagcaacgcc ctctttcagc acgaatactt gcgcaaaacc
    12181 agctttcgtc agcgcgtttg caggctcctg gcactgcatg ccagaaccgt ctaccacgat
    12241 aaccgatttg tctttgtgct tctcaagctc accaacattg ttggctttga tttcgctcgg
    12301 caacagattg atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc
    12361 cacaaccaca gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt
    12421 cactttcgag gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca
    12481 ggcgatactc agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa
    12541 caactcccaa cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa
    12601 atacagccag cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa
    12661 atctggcatc ggcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg
    12721 attcagctgt agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc
    12781 ggtttctaaa aaacctatgg tactggtgat tctggatggc tatggctatc gtgaagaaca
    12841 gcaggataac gccattttta gtgctaaaac cccggtaatg gatgcactgt gggccaatcg
    12901 tccgcatacc ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc gtcagatggg
    12961 taactccgaa gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac
    13021 tcgtctggac gttgaaatca aagatcgcgc tttctttgct aatccagtgc tggctggcgc
    13081 ggtacataaa gcgaaaaacg caggtaaagc agtacacatt atgggtctgc tctccgcagg
    13141 cggcgtacac agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg
    13201 cgcagaaaaa atctacctgc acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc
    13261 tgaatcctcg ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc
    13321 gtccatcatt ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa
    13381 agcctatgac ctgctgactc tggcgcaggg tgagttccag gccgatactg ccgttgctgg
    13441 tttgcaggca gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg
    13501 tgcagaaggt cagccagatg cggcaatgga agacggcgat gcgctgattt tcatgaactt
    13561 ccgtgctgac cgcgcgcgtg aaatcactcg tgcattcgtg aacgctgatt tcgatggctt
    13621 cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg accgaatacg ccgctgacat
    13681 caaaactgcg gttgcttacc cacccgcttc cctggttaac accttcggcg agtggatggc
    13741 gaaaaacgac aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt
    13801 cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc
    13861 accgaaagtg gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa
    13921 actggttgcg gccatcaaga gcggcaaata cgacaccatt atctgtaact atccgaacgg
    13981 cgacatggta ggtcacaccg gggtgatgga agcggcggtt aaagcggttg aagcactgga
    14041 tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac
    14101 cgctgaccac ggtaacgctg aacagatgcg cgatccagca acgggtcagg cacacacggc
    14161 acacaccaac ctgccagttc cgctgattta cgttggtgat aagaacgtga aagcggttgc
    14221 aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc
    14281 gcaagagatg actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa
    14341 ggcgattaat accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat
    14401 ctacgccagc gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga
    14461 gcgtgaccaa ctcaaatcta ttcaggccga tatcgccgca aaagagcacg cggtacgcca
    14521 aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc
    14581 tgaagccacc cgtaagctgc gcgaaacgca aaacacgctc aatcaactca ataaacagat
    14641 tgatgagatg aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag
    14701 cctcgccgca caactggatg ccgcattccg tcagggcgag cataccggta ttcagctaat
    14761 tctcagcggt gaagaaagcc agcgtggaca gcgtttacag gcttatttcg gctatctcaa
    14821 ccaggcgcga caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag tcaccatgca
    14881 gcgtgctgaa ctggaagaga aacaaagcga gcaacaaacg ctgttatatg aacagcgcgc
    14941 ccaacaggcg aaactgactc aggttctgaa cgagcgtaaa aagacgctgg cggggctgga
    15001 atcttccatc cagcaaggtc agcagcagct aagtgaacta cgtgccaacg aatcccgtct
    15061 gcgtaacagc attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac gagaagcgcg
    15121 tgaggcccag gcggttcgcg agcgccagaa agaagcgacg cgcaaaggca ccacctacaa
    15181 accgaccgaa agcgaaaaat cgctgatgtc ccgaactggt ggcctgggtg cgccgcgtgg
    15241 tcaggcattc tggccggttc gcgggccgac gctgcatcgc tatggcgagc agctacaggg
    15301 tgaattacgc tggaaaggga tggttatcgg tgcctctgaa ggtactgaag ttaaagcaat
    15361 tgccgacggt cgagtgattc tggctgactg gctgcaaggt tacggtctgg tggtggtggt
    15421 tgagcacggt aaaggcgaca tgagtcttta cggctataat cagagcgcac tggtgagcgt
    15481 tggttcgcag gttcgcgcgg gccagccaat tgcactggtg ggcagcagtg gcggtcaggg
    15541 tcggccttca ctctatttcg aaattcgccg ccagggtcag gcggtcaatc cacagccgtg
    15601 gttgggaaga taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt
    15661 ggcgctctcc tcccccgtac ttgctggcaa acttgccatc gtcattgatg attttgggta
    15721 tcgcccgcac aacgaaaacc aggtgctggc gatgccttcc gctatctccg tcgctgtatt
    15781 acccgattca ccgcacgcca gagagatggc gaccaaagcg cataacagcg gtcacgaagt
    15841 gttgattcat ctcccgatgg caccgttgag taaacagccg ctggagaaaa atacgctacg
    15901 cccggagatg agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc
    15961 ctatgccgtg gggatcaaca accacatggg cagcaagatg acctctaacc tgtttggtat
    16021 gcagaaagtg atgcaggcgc tggagcgtta caatctttac ttcctcgaca gcgtaaccat
    16081 cggtaatacc caggcgatgc gcgccgcgca aggtactggc gtgaaggtga tcaaacggaa
    16141 ggtgttcctc gacgattcgc agaacgaagc ggacatccgt gtgcaattta atcgcgcaat
    16201 tgatctggcg cgtcgcaacg gttcaaccat tgccattggg catcctcacc cttcaacggt
    16261 acgcgtgcta caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag
    16321 cagcttgctg aatgaaccgc aggttgatac ttctacaccg ccgaaaaacg ctgtgcctga
    16381 tgcaccgcgt aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc
    16441 tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat
    16501 cgtttacttc cagcatcagt ggcaaggctg gggcaaacag cacgaagcgg cgaagtttaa
    16561 cgctagcgca aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc
    16621 cattgccata agcggaaaga ccacagtaac gcctggtaac cgaccttcac gctgcgcaca
    16681 ttggtaatca ggcgcttata cataccggaa gtaaatatct cggcaatcat ccgttggcgg
    16741 gtaagaatat ccggctcttt gcgcaccgca tggcaaacac gcaatgcttc gtaagttatt
    16801 tgctgatgaa attccggata aatcgtaatt ttgtcggcat aatttcgatt taatttctcc
    16861 agcaggcggg taatcttaat atagtgacgt tgataattaa ggtttttatt cccttgtcta
    16921 tgcaaccgac tcactgacgt attatgcaga taatatttat ataatgattg ctcggtatat
    16981 cgcgcacgca gcgcgttaaa catgaattct gttgtccaga caatatcctg atgatgtaat
    17041 ccggcaataa atttaatgtt atttttaaca ataacatcac ggcgataaac ccccatccag
    17101 acaacgtgag tccaacggcg cgaagaaagc cccatccgca gccagtccgg gccggttaat
    17161 acaccggttg agcgaaggcg atcgctgggg atggattgcc aggtttctcc cgtttcacga
    17221 aaagaccagt cagcgttgca ctgcgccacg tcgaggtcgt cctctaacgc catggtcatc
    17281 agcgtttcgt acatggtggg atagacctca tcgtcagcat cgacaaaagc gacatatttg
    17341 cccgtcgcca cctcgatccc acgattacgc gctaccgatg cgccagcatt cgcctgatgc
    17401 aacaaacgaa cgtgcggata gttttctgcg taatgctttg ctatttcaac agaattatcc
    17461 gttgaaccat cgttaataat aatgatttcc agagcagtcc aggtttgcgt aattaaagat
    17521 tccatacaag tgcggaaatc atcgcccgca ttatataacg gaataataac actaagttta
    17581 ttggtgctgt tcatcataaa ttaccgacga cctctgaagt tatcctgacg ttttacaggc
    17641 tttgtattaa gaaactatta agcctgtgag atattatttt tacaggtgag agagtcactt
    17701 cagggtagag agataatgag aacagctata taaaagatca gcgcaattac gaactcttta
    17761 ttacactgac aggattttac ataattaaca gattatcctc agataaaagg gctggaatac
    17821 cagcccttgt tcgtgttaat cccagctcag aataactttc ccggactggc ccgaacgcat
    17881 agcgtcaaag cccttctgga aatcatcgat agagaaacga tgggtaatga tcggcgagag
    17941 atcgaggcca gactgaatca gcgccgccat cttgtaccaa gtttcaaaca tctcacgacc
    18001 gtaaatgcct ttaatgaaca agcctttaaa gatcactttg gtccagtcga tagacatatc
    18061 agacggtgga atacccagca tcgcaatacg accgccgtgg ttcatggtat cgagcatggt
    18121 acgaaacgct ggcggcgcgc cggacatttc cagaccgaca tcaaagcctt cggtcatgcc
    18181 cagttcagcc atcacatcat taaggttttc tttcgcgacg ttaaccgcac gggtgatacc
    18241 cattttgcgc gccagctcaa ggcggtattc gttaacatcg gtgatcacca cattgcgtgc
    18301 accaacgtgt ttcgccaccg ccgcagccat aatacctatc ggacctgcac cagaaaccag
    18361 cacatcttcg cccaccagat cgaacgacag cgcggtatgc acggcgttac cgaagggatc
    18421 aaaaatggaa gccaggtcgt cggagatatt gtcggggatt ttgaaagcgt tgaacgccgg
    18481 aatcaccaga tattcggcga agcagcccgg acggttaaca ccgacaccaa tggtgttgcg
    18541 gcacagatgg gtacgaccgc cacgacagtt gcgacagtga ccgcaggtga tatggccttc
    18601 gccagaaacg cgatcgccga ttttgaagcc tttcacttcc tgaccaatac ctaccacttc
    18661 accgacatat tcatggccca cgaccatcgg caccggaatg gttttttgcg accactcatc
    18721 ccagttatag atgtgaacgt cagtcccgca gatggctgtt ttacggattt taatcagcag
    18781 atcgttatgc ccgagttccg gtacagggac gtcggtcatc cagatgccct cttccgcttt
    18841 cagtttggat aacgctttca tctcacatcc tcaggcgata acgcccagtt gtttaccaat
    18901 acgcgtgaat gcttctactg cacgcgtaat ttgctcaggg gtatgcgccg cagacatctg
    18961 ggtacgaata cgcgcctgac ctttcggaac gaccggatag aagaaaccgg tgacgtagat
    19021 gccctctttt tgcagctctc gggcaaattt ctgcgccact accgcatcac caagcatgac
    19081 cggaataatg gcgtgatcgg ctcccgccag ggtaaagccc gccgccgaca tttgctcacg
    19141 gaactgacgc gcgttcgccc acagacggtc acgcaactcg ctacccgctt caaccatctc
    19201 cagcactttg atggaagcgg cgacaatcgc tggtgccagc gagttggaga acaggtacgg
    19261 acgagaacgc tggcgcagcc actcaaccac ttctttgcgc gccgcggtat aaccaccaga
    19321 agccccgccc agcgctttac cgagcgtgcc ggtgatgatg tcgacacgcc ccatcacatc
    19381 gcagtattca tgggaaccac gaccattttc accgacaaaa ccgaccgcgt gggagtcgtc
    19441 taccatcacc agggcatcgt atttatctgc cagatcgcaa acgcccttca gattggcaat
    19501 cacgccgtcc atcgagaaca caccatcggt ggcaatcagc acatgacgcg caccggcttc
    19561 acgcgcttct ttcagacgag cttcaagctc ctgcatatcg ttgttggcat agcgatagcg
    19621 tttagcttta cacagacgca caccatcaat aatagacgcg tggttcagtg cgtcggagat
    19681 aatggcgtct tccgcgccca gcagcgtttc aaacaggcca ccgttagcat caaagcagga
    19741 agagtagaga atcgcatctt ccatccccag gaatgccgcc agtttttgtt caagctcttt
    19801 atggctgtcc tgagtgccgc aaataaaacg caccgaagcc atgccgaaac cgtgagaatc
    19861 cattcccgcc tttgctgccg caatcagatc cggatgattc gccagcccga gatagttgtt
    19921 ggcacaaaag ttaatgacgt ggcttccatc agccacggtg atatctgctt gctgcgcaga
    19981 cgtaataatg cgctcttctt taaacaaccc ttccgcccgt gcggtttcca gatcgttggt
    20041 taactgctga taaaattctc cacgcattgc gattctccag acagggcaaa ttccagcaca
    20101 tattacccaa acttataggt cgcgacgaga tgatgcgtta acacttctgc aaaattcagg
    20161 ataaataacg tgtatcttga tgtctctcaa cacctacaga tagtagaaaa actgtacagt
    20221 tttggctgag cataatgatg agtgatttta ctgcattgat ataaaaagaa aaaatgatgc
    20281 caaagcatca tttttcctat ctcagttggc atcaaaacgc tacccactaa tggagggaaa
    20341 cctattttct tgaaaggaaa attctcagcg cctgcatttt tgcccctaaa gtgttactcc
    20401 tgaagcagcg aaacaaatcg aaccattttc ctctccccag atagtttagg ttgaagagat
    20461 cggaacgttt gtaatagcac atgacaaaaa tgccctggtc atcgtccaca atgttattgt
    20521 ttagtgttat tttttgactt tccagaacta atttatagaa ttctttccat ttatgctgtg
    20581 aaccaacaat tgcgccccca ataatatata catgattgcc aatcatgaaa tcaaaagcct
    20641 gttgttgaga tgtaacagta agcccttttt ttatagtgaa aagatgcatc ttattttcat
    20701 caaatgggaa atcccaaatt ttcaatcctc gagttacatt tggcttacgg caatagccaa
    20761 aatctatcca cgcaacaaga ggcgttttca ctaaacccat gttgatagct ttatttacaa
    20821 aataggactt aaggttacat accaaaacat actctggtga ccagtactct gggtttttta
    20881 attgtcgagg ctcaagtcta tttgtaaacg actcatcttt ttgaattttt tcgatacgac
    20941 ttctgatata tctaaatttc ttttttatat cgataacgat aaccgttgtt ggttttccgt
    21001 tgcgaatcgc ttcaatcctt ggtttcaaat cgggagaagt gaaaataatc atctcatttt
    21061 caagtgcggc cagacgctca aagtaactaa aataaacatc aactgaacga gctagttttt
    21121 cacggaaccc tttattagta gtccaatccc ctctaccaat gtcaaaatat gcagtgataa
    21181 ttgtggtgga tgatttcatt actaataaga ccattaatat aaaaaaagaa ttatatcata
    21241 aatattaaca gaatgtatac tgtatattat ccattcaaat gattccgtta tttatcatac
    21301 atcggaatat tgatactaaa gcactattca catgcaaaac caacatccgc catgaaggac
    21361 tagctaaaac ccaaactagt ttgttgcaat tagcatcctt gcacctctat gtaaagggct
    21421 gaagggattc ggatgtgatg gtatgattac agacattcgt gtctgagatt gtctctgact
    21481 ccataattcg aaggttacag ttatgatcat cgttaccggc ggcgcgggct ttatcggcag
    21541 caacatcgtt aaggccctga atgataaagg catcaccgat attctggtgg tggacaacct
    21601 gaaagacggc accaagtttg tgaacctggt ggatctggat atcgcggact atatggataa
    21661 ggaagacttc ctgatccaga ttatggctgg cgaagagttc ggtgatgtcg aagcgatttt
    21721 ccacgaaggc gcatgctctt ccaccaccga gtgggatggc aagtatatga tggataacaa
    21781 ctatcaatac tccaaagagc tgctgcacta ctgtctggag cgcgaaatcc cgttcctgta
    21841 tgcctcttcc gcagccacct acggcggacg cacctccgac tttattgaat cccgcgagta
    21901 cgaaaaaccg ttgaatgtct acggttactc aaaattcctg tttgatgaat atgttcgtca
    21961 aatcctgcca gaagcgaact cgcagattgt tggcttccgc tatttcaacg tttatggacc
    22021 gcgtgaaggc cataaaggca gcatggcgag cgtcgctttc catctcaaca cccagcttaa
    22081 taacggtgaa tcaccgaagc tgttcgaagg tagcgagaac ttcaaacgtg acttcgtcta
    22141 tgtgggcgac gtggcagatg taaacctgtg gttcctggaa aatggcgttt ccggtatctt
    22201 caacctcggc accggtcgtg cagaatcctt ccaggcagta gcagatgcta cgctggctta
    22261 tcacaagaaa ggccagatcg aatacattcc gttcccggat aagctgaaag gccgctacca
    22321 ggcgttcact caggcagatc tgacaaatct gcgcgcggcg ggttacgaca aaccgttcaa
    22381 aaccgttgct gaaggtgtaa cggaatacat ggcctggctg aatcgcgacg cataagagct
    22441 ctgcatgaaa atactggtga tcggcccgtc ttgggttggc gacatgatga tgtcgcaaag
    22501 tctctatcgc acgctccagg cgcgctatcc ccaggcgata atcgatgtga tggcaccggc
    22561 atggtgccgt ccattattat cgcggatgcc ggaagttaac gaagctatcc ctatgcctct
    22621 cggtcacgga gcgctggaaa tcggcgaacg ccgcaaactg ggtcatagcc tgcgtgaaaa
    22681 gcgctacgac cgcgcctacg tcttaccaaa ctccttcaaa tctgcattag tgcctttctt
    22741 cgcgggtatt cctcatcgca ccggctggcg cggcgagatg cgctacggtt tactcaacga
    22801 tgtacgcgtg ctcgataaag aagcctggcc gctaatggtg gaacgctatg tcgcgctggc
    22861 ctatgacaaa ggcattatgc gtaccgcaca agatctgccg cagccattgt tatggccgca
    22921 gttgcaggtg agcgaaggtg aaaaatcata tacctgtaat caattttcgc tttcatcaga
    22981 acgtccgatg attggctttt gcccgggtgc ggagtttggt ccggcaaaac gctggccaca
    23041 ctaccactat gcggaactgg caaagcagct gattgatgaa ggttatcagg tggttctgtt
    23101 tggctctgcg aaagatcatg aagcgggcaa tgagattctt gccgctttga ataccgagca
    23161 gcaggcatgg tgtcggaacc tggcggggga aacacagctt gatcaagcgg ttatcctgat
    23221 tgcagcctgt aaagccattg tcactaacga ttctggcctg atgcacgttg cggcggcgct
    23281 caatcgtccg ctggttgccc tgtatggtcc aagtagcccg gacttcacac cgccgctatc
    23341 ccataaagcg cgcgtgatcc gtctgattac cggctatcac aaagtgcgta aaggtgacgc
    23401 tgcggagggt tatcaccaga gcttgatcga cattactccc cagcgcgtac tggaagaact
    23461 caacgcgcta ttgttacaag aggaagcctg acggatgcgg gttttgatcg ttaaaacatc
    23521 gtcgatgggc gatgttctcc atacgttgcc cgcactcact gatgcccagc aggcaatccc
    23581 agggattaag tttgactggg tggtggaaga agggttcgca cagattcctt cctggcacgc
    23641 tgccgttgag cgagttattc ctgtggcaat acgtcgctgg cgtaaagcct ggttctcggc
    23701 ccccataaaa gctgaacgca aagcgtttcg tgaagcgcta caagcagaga actatgacgc
    23761 agttatcgac gctcaggggc tggtaaaaag cgcggcactg gtgacacgtc tggcgcatgg
    23821 cgtaaagcat ggattggact ggcaaaccgc tcgcgaacct ttagccagcc tgttttacaa
    23881 ttgtaagcat catattgcaa aacagcagca cgccgtagaa cgcacccgcg aactgtttgc
    23941 caaaagtttg ggctatagca aaccgcaaac ccagggcgat tatgctatcg cacagcattt
    24001 tctgacgaac ctgcctacag atgctggcga atatgccgta tttcttcatg cgacgacccg
    24061 tgatgataaa cactggccgg aagaacactg gcgagaattg attggtttac tggctgattc
    24121 aggaatacgg attaaacttc cgtggggcgc gccgcatgag gaagaacggg cgaaacgact
    24181 ggcggaagga tttgcttatg ttgaagtatt gccgaagatg agtctggaag gcgttgcccg
    24241 cgtgctggcc ggggctaaat ttgtagtgtc ggtggatacg gggttaagcc atttaacggc
    24301 ggcactggat agacccaata tcacggttta tggaccaacc gatccgggat taattggtgg
    24361 gtatgggaag aatcaagtag agtgtcgttc tacaagtatg tctcttgcag atttgccagc
    24421 tcaaaccgtt tttcaaaatt taaatcttga aataataacc aataagttga catcggagat
    24481 aagatgacct caacattatt tttctctctc gagaaaaaaa actggatagc gtactggaac
    24541 agagctctcg tattcttatt cattaccacc tattttttgg gtgggattac aaggtataaa
    24601 catcttattg ttattcttat gacaataacg acaatcgtct atctctgcaa acggccaaaa
    24661 cactatctct cactatttaa aacatttctt tttggtagtg ttgccatatt aactattgct
    24721 gcattgctgt cacttcttca atcccctgat gcaggtgcta gcatgaagga aattttcaaa
    24781 gctattattg agaatacttt actatgcaca atagcaatac cggtcatatt gagagacgag
    24841 aaaagagaag atgtcgaaaa aatcgttttc ttctcattta ttagtgcgtt gggcttacgc
    24901 tgtttttctg aattgattac ctattataag gactatcaac aagggataat gccattcgca
    24961 gattatagac accgtagcat ttctgactcg atggtctttt tattccctgc attgttaaat
    25021 ctctggctta tcaaatcagc aaaataccgc atttcttttg tggttctaag cgttattttt
    25081 atttttctga tattaggaac tttatccaga ggggcctggc tttccgtgtt agtcattgga
    25141 ttaatatgga ttctgatgtt taaacaatgg aagttactat tagtaggagt aatggtttcc
    25201 atcattgcat tgtcggttat tttcacacat aaggagatga ccgcaaagct aacgtataaa
    25261 cttcaacaaa ctaatagttc ttatcgctat gcaaatggta ctcaaggcag cgcactcgat
    25321 ctaatattag aaaatcctgt tattggttat ggttacggta acgttgcata taaagatgtc
    25381 tataataaac gtgtcattga ttatccagaa tggaccttta gacaatcaat agggccacat
    25441 aattttgcgc tattcatctg gtttggcact ggtttattag ggctggtaag tcttatgatg
    25501 ctatactgtg caatattgaa agagtgtata aaaaatggcg tcaagaataa atatcgctca
    25561 ccatataatg catattatat aatcttacta tcttttatag gttattttgt tatccgtgga
    25621 aacgtagaac aaattgaacc aaatttatta ggcgtttacg ccggcttatt attagcgatg
    25681 aaaaacaagt aaaaaataaa aaaggctgca taatgcggcc tttttattta ttcaaaccaa
    25741 ttatgaataa cctcttcgaa tctttgagtt acgccatccc aactgtagtg atcaaacaca
    25801 aagtcctgac cttgctttgc aactgctgtt aattcaggat tcgctaacgt ttttaaaata
    25861 tcgctgctta tggagtctgc agtcataggt tcttttaaat ggaatcccgt agtattttct
    25921 ttgacaaatt ccgtcatccc cccacgcgta ctcactaaaa caggcttacc agcacccata
    25981 gcttcaattg ccaccatgca aaatggttcc tggaactggg aaggaatgac gactaaatct
    26041 gccagtgggt aataacaatg catcttttca ggcggtatgc ttcctaacat aatgcaacga
    26101 tccttcaaac gttttgcaat ttccctgaca ttgcgttgat atgcaccttt gtcactttta
    26161 ctcatttctg tataatcacc aatcacaact aattttaaat ttttgtgagc agcggccaat
    26221 ttttcgaatg attgcatcaa cagcaatatg cctttgtcag ggacgatacg cccagcataa
    26281 aaaataattt tctcttcagg ggagatatta agttcgcttc tagtgatcgg ctgaaaatta
    26341 gattgatagg tttctaaatc aattccattt ggtacaatct caatatctgc atttgctaaa
    26401 taagattggt aatacttttt caagtataaa ctagggacta tcattttaac attttgctca
    26461 agcaattttg gttcaaatgc attatgcata tgtataacca ctcttgcctg aggagctcgc
    26521 ttacgaattt gagtgtatag cttcatgcta ttatgaacta caataacact ttctttcgtt
    26581 ataggaaaat catgtgcaat attcaatatt ctctgtgaat atggaagagg atcaagtctt
    26641 gtccattttt gaaataaccg cttatatatg cgactgaatc caaccctatg aatactacaa
    26701 ttatcattaa caaaggtgta atttgagtat cctggatttt tgatacaaac aatacgattt
    26761 ggaaaattgg tacgctgagc aacctgatac atccacgttt caacagcagc cgctccgcga
    26821 ggaggaattg aaaaaatagg agtaaccgta aatattattt tatcaaccat ctcgtatatt
    26881 aacctttcat aacattatat ttaattgctg taaaaacacc atcgagaaat tttttctggt
    26941 aaagcaagtg tttatatttt tctttgtgct catgtttttt aacagctttt ttataaggta
    27001 tatttctcca tggtgatata ttataaatat tacgaaaata atctgcagag gcgtagttcg
    27061 cccaatcatg ccatggctta gttataccag tataatgtat gaagacagtg tcatcattaa
    27121 taaaccgggt gtaatattca ctattttttt cttcaaattc tgactttata gtataaatac
    27181 aattatattt tctcggtaat attttagcat gatgtagaaa cattaagttt aaaatatctt
    27241 gatccgggta ctttaatttt tttataattg actcatctga taatagatca aagaattttt
    27301 ccgttagtct ttgttttaac cattcccgta aattaatata cattacacca gagttaaaat
    27361 agctaccgtt aaaatcctca ttacacaaac gctcagcgct tttactttgc atagcatcta
    27421 catcaattac cactgcccca tattcatctt taaactctaa tgctattaac tcgttcaatg
    27481 aacccttaca gacgatatca gcatcaagat ataataatga atccaatcgc gcagaaaaat
    27541 aatcaaatga aagcaaacga taataaatag atactggcca gatatttgat tgaggtagcg
    27601 ccttcaatgg ctcagaatca atatgatata atttaattac tgtttgatac ttcgttgcta
    27661 attgtgataa taattcaata tactctttgt ctaagtaatc agcgattatg tgaaaggtaa
    27721 atttgttttg tttattcttt tcaagaattg aagcaattga aatcgcagcc ccaacctggt
    27781 agttctcatc aataccccat gagacattaa acgtttcccg ttcatcaagt ttaacggggc
    27841 gcttgtccag ttcaattata tctttaaatt gagtaagatg tttaaaatcc aatttaatta
    27901 cccttcatca taatcattat ttgctcttca atcctagttt gacttttaca tccctgatta
    27961 cttttttaat tttctttttg aatataagat atgtatagcc aaagtctttc aattcatttt
    28021 ttatattata gtgccgttca agatcaatgc gatcttttgc ttttaaaaca gcggttgttt
    28081 ttttacctga aaggtcaatt agtcttaaac ctttttcgga tactataaaa tttcctttat
    28141 gaggatcgcc agacaccatt ccatgctgat gcaactcttt tatcgattct gataattgat
    28201 ccttcagatc ttcagaaatt tccaaatact catttaaccc gacaccttca atatactcaa
    28261 tgagcataat ataataatga gcgaaattta atgttttacg ttcagctagc aaaaaatagt
    28321 cattaattga ttggatgcct tcaccacgta cccgatcggt ttgataaatt aaattttcat
    28381 aataatcttt ttttatgcaa gattttaaga aacgttcggt cattttatgc tttggcgcat
    28441 aaactttaag tacaagaggt ccacgagcag tgtcaataag aatgacttta gtatcatcaa
    28501 tagaacgaaa aacttttaat gttttaaccc tgcaagttaa aaagtcattt agaacttgct
    28561 cataaaaggg atcattatct tttatgaaaa ctttcaaccc atttatcgtt ttattatata
    28621 tcataatgaa ttattttaac cttaaatctt tagaagcatt tttctttata atactttaaa
    28681 taattaaaaa tgcctgccat ataatgcttt tgtttaaatt tatgttttgc acaataccga
    28741 tactgattgc tatttacagg tttaagtaaa tcttctttac tccacggaga agctgctttg
    28801 gcgatcaaaa aactacgtga aaccggataa tcagcccact catgccaggg ttttgtaggt
    28861 cccacatagt gtataaacac ggtgtcatca ttgactggat tatcaacttt gtcttttaat
    28921 tcatagttaa tactatatcg ggtattatat ttctccgaaa taaacttcac tttaccattc
    28981 aataatacat tcagtacatc ttgatcaagg tgggtgattt tacttaccca atctgggtca
    29041 cgcaacattt caatagcttt tgacgaaatg ttatttagat tccactcatc aatatttatc
    29101 agtaaaaaac cagcattgaa atatccagaa gctaactgtg gtgtagttaa taccgaggct
    29161 cgattttgcc accattcaat atctctttca gctacaactg cggcaatttc attagtagaa
    29221 aattgataat ctaagagttc tttaatacta cccttgcaag caatatctgc atcaagatat
    29281 agtatttttt catgtttatg atagaaataa tctgcaatta taaatcgaaa atatgttgcg
    29341 taagtccagt ttttcgtgct tggtaatgac ttcagcttat cacaattgat aacataaata
    29401 ttaattcttg aattatattg ttttgctaaa tcagaaaaat ataatttgtc tttatcactg
    29461 atataatctg tgaaaacatg aaattcacaa gagatttctc tattgtttaa tagaatagat
    29521 gcgatggcta caccacatcc aaaaagaaaa tttctgtcaa taccaaatgc tatatcaaga
    29581 gtatttttag atcttatatt ttgataatga aaattatact caaaaaggat gatgtcacta
    29641 tcattgagtt gagacatatt tattctgtat ccaaaatttt ggcaaaaata agttacgggt
    29701 tacaacgatt ttcgaatcgt tctttccctg atttttgtgg cttttgcttc tgcttgcgac
    29761 agcagtcctt gttcctgctt gagaatgtct ttaagcgggg cggcaaaata caccttcata
    29821 aagcgccaga tatcccgctg agtcaggcca atattcattg aagaaaaata aagccctata
    29881 agatctttat cgcgccaacg acctggaacg cgcgtgcgaa gctgcgcccg gtgtaggtca
    29941 attaccgaaa tttttaactc ctcttcctta ccggaaaaag gcaagtgcag caggaaatga
    30001 cagatataac agtcacgatg gttaatgccc gcagcatgca tatcgcgcac catcgtcgcg
    30061 acacgcttaa taagcatacg ctttacgcga acatctggtg ggttggtcgc ccagtcagca
    30121 cagtaatctt ccagactgat ggttggtgtc agatcttcgg taataataaa cgaggttctg
    30181 gtcagcggat tcatgccttt ttcgccgaat gccaccccat acatagtatc aacgccgaca
    30241 tcccgcagtc gatgaatcgc attccattcg cggtctgcgc ctaatactgg catccgcaat
    30301 gagagtaaat ttttgattat ctctttcagg gtcgtgccgc gatgccattt gagaaaatag
    30361 ctttttcccg ccatttcaaa gcgcagagta cggcgagttt ccagttcacg aaatacctca
    30421 ccctgcaagg ttttaacttc ctcaaaagga tctttgccgc gccataacgt ggcaaacggc
    30481 tctttaagtt caaccatcca gaccacccgt tatgatatcc gccgctttct ctggcagact
    30541 gtataaatct tgtgtatcag cataatgtcg cgcattttcc gcccaagcct ggcgcaatga
    30601 agattgcgtt aacgctttgc gtaaaatctc attcaatgtt tcctggcgga atggctcagc
    30661 aatagcctcg ccgcaattag cgtcgacaat ataatgcgca tagccacaaa cggcagttgt
    30721 tagtaccggt aatcctgcag taatcgcttc cagcagcaca attcccgccg cttcctggta
    30781 ggcaggatgc agtaataaat ccgccgccgc cattaattcc gagacatcgt tgcgccccga
    30841 gaagaagtga acattactgc gcacgccgcg tttttctgcc agtgcctcaa attttcgcgg
    30901 tttatcctgc ccaacaacat atagcaatgt gttgtggcgc agcgaatccg gtaacgaagc
    30961 aagtgcttca atggaacgat cgacaccttt acgcgtgaag tctgaaccga cctgcaacaa
    31021 taaatattgt tgttcggtta ttccattctt cttacggaag atttcacggc tattggctgg
    31081 ctgctggcta tatttacgat caggataaat ccccggtggc agaatatgaa aacgctccgc
    31141 ttcagtctga taatgtttct ggaaatcggc gatttgctta tctgtcagca tcagcagctg
    31201 tgtcggcttg ccctgttcga aggttgcccg ctcaaaggcg gcataatggc gataacgtga
    31261 cgtcaggcga tagaaaaagc ctttttcctg cgctactttc tcggcataac aaacatcagc
    31321 ggcataataa acgtccagcc ccggcatttt gttgaatcca acgactttat cgacgggatg
    31381 ttcgcgtaaa tgtttttgca cccaggcaaa atactccgca ttgcgcccgt gattggtatg
    31441 cgatttaacc ggcactttga tcagttcaaa tacatcaggg cattcgcctt cccacgactg
    31501 ggtataaacc cgaacatgat gacctcgggc ggcgactgtc tgagcaatac gcataaaatc
    31561 gcgctgcaaa ccgccaaagg gaaaatattt atataaacaa aaagcaacga tcataacgac
    31621 gtacctgtcg tggaggaggg cagtaattta tcgacagcag caatgacatc tgccgccgga
    31681 ataaccgaaa gatacatctc atttcggtca cgctgatcgc gcgttggcat ttcccggtaa
    31741 tctcccgccc agaattgaat catgttattt gaccagggac gccagaaaat atgatctgtt
    31801 gcgccaaaca gcgatatcag cggcgtatta actgcagcgg caatatgcgc cggtgcggaa
    31861 tcaacgccaa taaacagctg cgcatgatcg attaacgcac caagttccgg gaaggtcacc
    31921 tttccagcca gcgccgttac tggtggcgtc tggcatccct gcgcaatttc attgacgcag
    31981 gccagatcgt ctttatctgg gccggacgtc agaacgactt cataaccacg agcatgtaag
    32041 gcatcaatca cagcggaaaa cttggcgttg tcccagcatt tgaagatttg ccgcgccgta
    32101 ggttggataa ccacataatt ttgtccaaca ccagcgtgat caagttcgcg acgcatacgt
    32161 ttccagcttg caggcgggta actcatggtt gtctgcttca ccaacgaatc aagtcccaat
    32221 ggtgtcagca cggataagtt actttccacc acatttccac cctgcaacgg caccaaatgg
    32281 gtgaaacttt tacgccaaaa agcagactgc cgatgatgat aatcctggga aattttcaca
    32341 cgagcattta ataagcgaac cagtatagca accatccatt gatcggtaag attgacgata
    32401 aggtcatact tattggcacg taataccttg atgagatgaa aaaagttggc aattttttct
    32461 gaggcttttg cttttttatt ttttatgccg tagagcgcgt taatctctgg attttcagac
    32521 aggatcggga tggtgtcctg ataaagcagc acatcgattt ttgcgtcagg gtaatttttt
    32581 ttcagagaac taatgacggg agtagttaat aacatatccc catgaaaacg catcttaatg
    32641 agcaaaattc ttcgaaatgg cttatccaca agtaactctt tttgttaaga ttatccgatt
    32701 aaggtgaagc agaaaaagca cgctaccgcc ccaggctcaa cagctacctg aatactgata
    32761 ccgcactgat attgacgcgc tagtgtatca cttctttcgc ggcaatccga cccgaatgat
    32821 ttttaagcac attgttgatt ttcaataaag aaatcaatca aataaaatca ttagcaacca
    32881 tattctttca ctgttgctct gcgattcctt cctgcacgta cagcagaaat caatgaatcg
    32941 cataaaaata caaaaaaagt atagatgttc agcaagaatt ggcgggtaaa ttgctattta
    33001 agtca
//
LOCUS       DAJTYF010000032        32688 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTYF010000032 DAJTYF010000000
VERSION     DAJTYF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.032"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..937)
                     /locus_tag="OO590_001647"
     CDS             complement(<1..937)
                     /locus_tag="OO590_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR6637051.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFMINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVD
                     KIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNREGNYNIIDS
                     VDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKL"
     gene            complement(1037..1948)
                     /gene="gsiD"
                     /locus_tag="OO590_001648"
     CDS             complement(1037..1948)
                     /gene="gsiD"
                     /locus_tag="OO590_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6637052.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1951..2871)
                     /gene="gsiC"
                     /locus_tag="OO590_001649"
     CDS             complement(1951..2871)
                     /gene="gsiC"
                     /locus_tag="OO590_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6637053.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2889..4427)
                     /gene="gsiB"
                     /locus_tag="OO590_001650"
     CDS             complement(2889..4427)
                     /gene="gsiB"
                     /locus_tag="OO590_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6637054.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(4447..6318)
                     /gene="gsiA"
                     /locus_tag="OO590_001651"
     CDS             complement(4447..6318)
                     /gene="gsiA"
                     /locus_tag="OO590_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6637055.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6305..7270)
                     /gene="iaaA"
                     /locus_tag="OO590_001652"
     CDS             complement(6305..7270)
                     /gene="iaaA"
                     /locus_tag="OO590_001652"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6637056.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7474..8709
                     /gene="moeA"
                     /locus_tag="OO590_001653"
     CDS             7474..8709
                     /gene="moeA"
                     /locus_tag="OO590_001653"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6637057.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPISATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8709..9458
                     /gene="moeB"
                     /locus_tag="OO590_001654"
     CDS             8709..9458
                     /gene="moeB"
                     /locus_tag="OO590_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6637058.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9637..10299)
                     /gene="fsa"
                     /locus_tag="OO590_001655"
     CDS             complement(9637..10299)
                     /gene="fsa"
                     /locus_tag="OO590_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6637059.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10430..11329
                     /locus_tag="OO590_001656"
     CDS             10430..11329
                     /locus_tag="OO590_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6637060.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11335..13767
                     /locus_tag="OO590_001657"
     CDS             11335..13767
                     /locus_tag="OO590_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6637061.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13913..14728
                     /gene="ybiV"
                     /locus_tag="OO590_001658"
     CDS             13913..14728
                     /gene="ybiV"
                     /locus_tag="OO590_001658"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6637062.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14880..16145
                     /locus_tag="OO590_001659"
     CDS             14880..16145
                     /locus_tag="OO590_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6637063.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16386..17978)
                     /locus_tag="OO590_001660"
     CDS             complement(16386..17978)
                     /locus_tag="OO590_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6637064.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPECVIDFSGNYEDYLRSKGIE"
     gene            18197..19117
                     /gene="ldtB"
                     /locus_tag="OO590_001661"
     CDS             18197..19117
                     /gene="ldtB"
                     /locus_tag="OO590_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6637065.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(19175..20293)
                     /locus_tag="OO590_001662"
     CDS             complement(19175..20293)
                     /locus_tag="OO590_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6637066.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20290..20757)
                     /gene="mntR"
                     /locus_tag="OO590_001663"
     CDS             complement(20290..20757)
                     /gene="mntR"
                     /locus_tag="OO590_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6637067.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20943..21071
                     /gene="mntS"
                     /locus_tag="OO590_001664"
     CDS             20943..21071
                     /gene="mntS"
                     /locus_tag="OO590_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6637068.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21343..22926
                     /gene="opgE"
                     /locus_tag="OO590_001665"
     CDS             21343..22926
                     /gene="opgE"
                     /locus_tag="OO590_001665"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6637069.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22974..23489)
                     /gene="ompX"
                     /locus_tag="OO590_001666"
     CDS             complement(22974..23489)
                     /gene="ompX"
                     /locus_tag="OO590_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6637070.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23542..23607)
                     /locus_tag="OO590_001667"
     CDS             complement(23542..23607)
                     /locus_tag="OO590_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637071.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23842..24729
                     /gene="rhtA"
                     /locus_tag="OO590_001668"
     CDS             23842..24729
                     /gene="rhtA"
                     /locus_tag="OO590_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6637072.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            25028..25531
                     /gene="dps"
                     /locus_tag="OO590_001669"
                     /gene_synonym="pexB"
     CDS             25028..25531
                     /gene="dps"
                     /locus_tag="OO590_001669"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6637073.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25935..26681
                     /gene="glnH"
                     /locus_tag="OO590_001670"
     CDS             25935..26681
                     /gene="glnH"
                     /locus_tag="OO590_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6637074.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            26820..27479
                     /gene="glnP"
                     /locus_tag="OO590_001671"
     CDS             26820..27479
                     /gene="glnP"
                     /locus_tag="OO590_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6637075.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27476..28198
                     /gene="glnQ"
                     /locus_tag="OO590_001672"
     CDS             27476..28198
                     /gene="glnQ"
                     /locus_tag="OO590_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6637076.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            28459..30683
                     /gene="ybiO"
                     /locus_tag="OO590_001673"
                     /pseudo
     CDS             28459..30683
                     /gene="ybiO"
                     /locus_tag="OO590_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(30680..31606)
                     /gene="rlmF"
                     /locus_tag="OO590_001674"
     CDS             complement(30680..31606)
                     /gene="rlmF"
                     /locus_tag="OO590_001674"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6637077.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            31882..32142
                     /locus_tag="OO590_001675"
     CDS             31882..32142
                     /locus_tag="OO590_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6637078.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            32407..>32688
                     /locus_tag="OO590_001676"
     CDS             32407..>32688
                     /locus_tag="OO590_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637079.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDA"
BASE COUNT         8012 a         8727 c         8090 g         7859 t
ORIGIN      
        1 aaagtttatc tgatgcgttc aataaattac cgccaggata ataaaaatag atttcgaaca
       61 atgcattatt tggcactgct gatgtctttg caatatactc cgtatcgaca gaatcaataa
      121 tattataatt accttctcta ttataaataa tcagtttttc acgagcgagt tgattcttgg
      181 caatgatctc atgaccttgt ttcaaaaata gaacttcatc attagcgctg atttgatcaa
      241 gatactcgtt cagataccgc cagtttaaat cgacatgaat tttatctacc gctaacaaat
      301 cgtgatcttt gccgttgata cgtagcgtaa aagagataca ttttttaccc ggacgatcaa
      361 agcaattgta tgccggattc cagctgttgt tagagtcatc tgaagccacc gaccacggtc
      421 gtttcgatgg gttaaaatcg gcaaaaataa cccgccctga ctcgtcgaca agcggtccat
      481 ttacttgttc ctgatgataa acaacgattt tatcattcgc cctccgcgaa aagagataat
      541 atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt tccattttca
      601 gaatcacggc gcgttgaatc tcctctgtat tttttaataa atacagtgag tcctttaatg
      661 tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga ataagttcaa
      721 agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta atcatgaaaa
      781 aaagaccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt atctttatct
      841 tttcgtataa actcagcatg ttaatacatt taacaaaata actatctgaa cgctgtgccg
      901 ctgcaaaaat agtcccttgc tgcggctttt taaccaaatt acccgattct acaaaatcag
      961 cagaaagtta caacacttgc ttagggcaat aatttcctgt gaggtgatta ccctttcaag
     1021 caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt aaaccatcgc
     1081 ccaacaaatt gaacgccagt acggtcagaa aaatagccag ggccggaaaa acagcgacat
     1141 gcggcgcgat aaccatatcc gctcgtgcct cattgagcat tgctccccac tctggtgtcg
     1201 gcggctgcgc acctaggccg agaaatgaca ggctggcggc agagataatc gaggtgccaa
     1261 tgcgcatggt gaaaaacacc acgatagaag agacggtccc aggcaggata tgacgcaata
     1321 aaatggtcat atcgctggca ccaatactgc gtgctgactc aataaaggtt tgctgtttca
     1381 acaccagcgt gttgccgcgc accaggcggg caaacgcggg gatggaaaaa atggcgactg
     1441 cgataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc gccagtaaaa
     1501 tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc caccagcctt
     1561 cataatatcc agccaacaag cccagcaacg tccctatcgc cacaccgata aacacggcaa
     1621 acacgcccgc cgccagcgag atttgcgcac caaccaggac acggctgaaa atgtcgcgcc
     1681 ccagtgaatc gacgccaaac cagtgctgca aagaaggtcc gttattcaga ttgtcataat
     1741 caaaataatt ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc accacaatca
     1801 ataaaataac gaataatgcg gcggtcatcg ccatatgctg acggcgaaat cgtcgccaga
     1861 attcatgcca cggtgtacgt acctggtcag gtttgaccag tggcatggcg tttaacaccg
     1921 cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg ggttaatggc
     1981 ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga aaagcagaat
     2041 ttccgcctga atcaccgggt aatcgcgcat ttctacagag tcaacgagta agcgtccaag
     2101 tccgggccag ttgaaaactt tctccaccac gatagaacct ccgagcaaaa agccaaactg
     2161 taagcccatc atggtcacta ccgggatcat cgcgttgcgt agcccgtgtt tgaggacaac
     2221 ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc ttaaaacgtc
     2281 gacaaatgac gcacgggtaa agcgcgccat cacggcggcc actgccgcgc cgagcgtcag
     2341 ggagggtaaa atgtagtgct gccaactgtc tgctcccaca gtaggcagcc agcccagttc
     2401 aacggagaat acctgaatta aaaacatccc cagcgcaaat gccggaaacg agatccccga
     2461 caccgcaatg gtcatactca atcgatccgg ccaacgatta cgccagacgg cggcgataat
     2521 tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca gccacagcgt
     2581 tggcataaag cggctggcaa tctcatcggc aaccggacga cgcgacacca tcgacaggcc
     2641 aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgataca gcggctgatc
     2701 caaccccagc tgctgacgca ccagttctat aacctgcgca tcagcttcgg gcccggcaat
     2761 caatcgcgcc ggatcgccgg gcagcatgtg gacaaataaa aacaccagca ccgagacgat
     2821 aaacagcgtc ggaatcaacc ccagtaatcg tttgataacg taattaagca ttccactccc
     2881 tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc cgctgtctgg catgatccaa
     2941 aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag cgggatccac
     3001 ggcgattctt gccagatgat atcctgcgcc gccttatata agcgggtctt ttccgccgga
     3061 tcattcgttt tcagtgcctg agccaggaag tcatccacct gtttattgct gtaaaacgcg
     3121 gtattaaaca gcgtcggtgg ccagttttgc gaggcaaaca gcggcgatag tgcccagtcg
     3181 gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgccgct ctctttttgc
     3241 cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcggtcac ctgggcttta
     3301 atcccgacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc ggtgctgtgg
     3361 ttatgtgacg accacagcgt ggtactgaaa ccgttgggat atcccgcctc tttcagtaat
     3421 tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta ggcgatactt
     3481 ggcggtacca caccagtagc tggcgttgca tagcccgcaa aggcaacttt caccagcgcc
     3541 ggacggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa cggcttttgc
     3601 gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa ctcgatattt
     3661 ttgtttttct ccagcagtgt ggcctgctcg tagggaatgg ggaaagcaaa ctgcgcttca
     3721 ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca ggttatgctg
     3781 tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt cacaaaatcg
     3841 gtctgattcc aggtatccag ttcatacggt ccggttccca ccggatgaaa accaatctcc
     3901 ttgccatatt tttccagcgc tgtcggtgaa atcatcgcgg tcgccggatg ggcaagaata
     3961 ttaataaacg ctgagaacgg ctgtttgagg gtaatcttta ccgttgtcgg atcgatcgcc
     4021 tccgttttgg caatattctt atacaggtta tagcgtttaa gatgattcgc cggatcgctg
     4081 gcccggtcca gattcgcttt taccgccact gcgttgaaat cggtgccatc ctggaattta
     4141 attccttccc gcaatttcac ggtgtaagta atgccgtcat cggaaacggt ataactctcc
     4201 gccagcacgt ttttcagttt catctcttta tccagaccga acagcccctg gtaaaacgat
     4261 ttcgctacgg cctgagataa cgtgtcattc gcgtcatacg gatcgagcgt ggtgaaattc
     4321 gatcctaccg ccaccaccac atctttggca gcgaatgcac aagatgccat caacgctgtc
     4381 gcaatgccca gcgccactaa cccactacgg tgtacagctc ttgccatttt attctccgcc
     4441 tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt aatgccccgg
     4501 cccgacgcat tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa tattgctggg
     4561 aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag cgaccggaac
     4621 tgccgccagt aatttacgcg tataaggatg ctgcgggttt tcgaagaccg cgcaccgtga
     4681 accaatttca acaatttgcc cgagatacat caccgccaca cgatgactaa tccgctctac
     4741 cacagccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct ggagatcgag
     4801 caacaagttg ataatctgcc cgcgaataga aacatccagc gccgaaacgg cttcgtcggc
     4861 aatgatcact tttggattca atgccaacgc gcgagcaatg cagatgcgct ggcgctgacc
     4921 gccggaaaac tcatgcgggt aacgccaggc atgttcaggt aacaggccca cgcgctccag
     4981 caaccacgca acgcgtgcag ccgcgtcttt acctggcaat aaaccgtgta cacgcagcgg
     5041 ttcgataatc gaatcaccga tggtctgacg tgggtccagc gaagcgtaag ggtcctgaaa
     5101 aataaactga atatcccggc gcaatgcctg aagtttgcca ggtgacaagg tatcgattcg
     5161 ctgaccgtta aagataattt cgccgccctg cgattcgacc aggcgcagca acgcccgccc
     5221 ggtagtggat ttaccgctgc cagactcgcc caccagcgat agcgtttcgc caggccagag
     5281 atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca acaaaccgct
     5341 gcgcaaaggg aaacgggtga caagattacg cacccgtaaa acaggttcgc catccaccac
     5401 cgttttctgc tcgatggggg gttcctgttt cgctggatgt tcaagcgata tcaatgggaa
     5461 acgtcgggga taatctaacc ctttcatcgc accaagttgc ggaacagcag ctaacagcgc
     5521 acgggtgtaa ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg tttccaccgc
     5581 ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacgc ccatatcgtg
     5641 agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta attgcaggat
     5701 ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggcaatca gcaccgccgg
     5761 gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag agagttgatg
     5821 cgaataacgt gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca gcatccgctt
     5881 cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaattg attcggcaat
     5941 ctgttcaccc acagtaaata ctgggttcag cgatgtcatc ggctcctgaa atatcatcgc
     6001 catatccgca ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa gttcaatcac
     6061 atcacgactg cgccgccgca acagcatttt atcgcactgt actaaaccgc ccgcctgttc
     6121 caacagacgc atcaatgcca gcgcagtcac tgacttaccg gagccggatt cgcctacaat
     6181 tgccagcgtc tcaccgcgtt gcagactaaa agagagattg cggaccgcag ctattttctg
     6241 ctggtcctgc ataaaggcaa tattaagatt ttcaaccgcc agcacattac cggcatcaag
     6301 ttcatcactg tgtggcaacg gtgtcccctt tttcacggta gataccggtg gttggcgtat
     6361 cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc gcgacattcc
     6421 cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcagggagt ttttccatta
     6481 ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac gcggcgatgt
     6541 catatgccgc cagcgcgcgg atgaagactt cgcctgtgcc ggtacaagaa accgccacac
     6601 tggcgttatt ggcgtagcat ccggcaccca ctaagggact atcgccaact cgtccgggta
     6661 atttattggt cattccgcct gtggacgtgg ctgccgccag attgccgtct aaatccaacg
     6721 ccacggcccc cacggtgccc attttctgtt tttcatccag tggcgcaccg ctatggtcga
     6781 ggactgttgc cccttcctcg cgcgctgcca gtagttgttc ataacgcaaa ggcgtggaga
     6841 aaatctccgg tgagacgcgc tccatgccat gagcaaacgc aaaattttct gccccttcgc
     6901 caatcatcat cacatgcggg ctttgctcca tcaccagccg ggcggcaaga accggattac
     6961 gcagatgact aacgcccgcc accgcaccgg ctttcagggt gttaccatcc atcacacagg
     7021 cgtccagttc atgggtttca tcacgcgtaa agacagcgcc aattccggcg ttaaacagtg
     7081 gacactcttc cagcagacgc accgcttccg tcaccacatc cagcgcactt tcgccggctt
     7141 ccagcatttt ctgcccggtt tcaacaatgg cagacaacgc ctcgatgtag cgtaattcct
     7201 gttgcagact catctgcgcg cggctaattg cacctgcgcc accatgaatt gcaatgactg
     7261 ctttgcccat agtattcgtc cattaagggt ggctcgccac ttttgctata aatatcagaa
     7321 tttttatgaa ttactcattc aatttttgag tatagaaaga tggatatggc atgtaaaggc
     7381 aggagccaac gctacgcccg aggtggcata cagtctatgg cgtttttctg acataatagg
     7441 caaattcgat tttgcctccg caggagtgtt ttcatggaat ttaccaccgg attgatgtcg
     7501 ctcgacaccg cgcttaatga gatgctttct cgcgttaccc cactgaccac ccaggaaacg
     7561 ctgccactgg ttcagtgttt tggtcgtatt ctggcgagcg atgtcgtttc gccactggat
     7621 gtcccggggt ttgataactc cgctatggac ggctacgcgg tgcgtttagc cgatattgcc
     7681 tccgggcaac cgctgcccgt tgccggtaaa tcctttgccg gtcagccata ccatggtgaa
     7741 tggcctgcgg gtacctgcat tcgtattatg accggtgcgc cggtgccgga aggctgcgaa
     7801 gcggtggtga tgcaggagca gactgaacaa acggacaatg gcgtgcgttt tactgctgaa
     7861 gtgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg tgcggttgtt
     7921 ttcccggcgg gaactcgcct gactaccgca gagctgccag taattgcttc actggggatt
     7981 gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg tgatgaactc
     8041 cagttgcccg gtcagccgct gggcgacggc caaatctacg acaccaaccg tctcgccgta
     8101 cacctgatgt tagaacagct gggttgcgag gtaatcaact tagggattat ccgcgacgat
     8161 ccacatgccc tgcgcgccgc atttattgaa gccgacagcc aggcggatgt agtgatcagt
     8221 tccggcggtg tttcagtggg tgaagcggat tacaccaaaa cgattcttga agagctgggg
     8281 gagatcgcct tctggaagct ggcgattaaa ccaggtaaac cgttcgcatt cggtaaactc
     8341 agcaatagct ggttctgcgg cctgccgggc aacccgattt cagcgacgct gaccttctat
     8401 caactggtac agcctttgct ggcaaaacta agcggcaaca ccgccagcgg cctgcccgcg
     8461 cgccagcgcg tacgcacagc gtcccgcctg aaaaaaacgc caggacgtct tgatttccag
     8521 cgcggcgtgc tgcaacgcaa cgccgacggt gaacttgaag tgacgaccac cggacatcag
     8581 ggttcacata tatttagctc ctttagtctc ggcaactgct ttatcgtgct ggaacgcgat
     8641 cgcggcaatg tggacgtggg cgaatgggtg gaagtagaac cgtttaacgc gttgttcgga
     8701 ggcctgtaat ggcggaactc agcgatcagg agatgctgcg ctacaaccgg caaatcattc
     8761 tgcgcggttt tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg ctggtagtcg
     8821 gcctcggtgg actcggctgc gcagcttcgc agtatctggc aagcgccggt gtcggcaacc
     8881 tgacgctgct cgacttcgac acggtttcgc tctcgaatct gcaacgccag acactgcaca
     8941 gtgatgccac ggtcgggcaa ccgaaggtgg aatccgcccg tgacgccctg acgcggatca
     9001 acccacatat cgcgattacg ccagtcaatg cactgctaga tgacgcagaa cttgcagcat
     9061 tgattgctga acacgatctg gtgctcgact gtacggataa cgttgcggta cgtaatcaac
     9121 tgaacgcagg ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca attcgtatgg
     9181 aagggcaaat cacggtcttc acttatcagg acggcgaacc gtgctatcgc tgccttagcc
     9241 gtttgtttgg cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca ccgttgatcg
     9301 gcgtaattgg ttcgttgcag gcaatggaag cgatcaaact gctggcaggt tatggcaaac
     9361 cggccagcgg gaaaatcgtc atgtacgatg cgatgacctg tcagtttcgc gaaatgaaac
     9421 tgatgcgcaa tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca ggcctgataa
     9481 gacgcgacaa gcgtcgcatc aggcatttgc tccagacgcc agatgcggcg tgaacgcctt
     9541 atctggccta cggttcgggc acagatttcc aagcgcggca ggcatcggtg cactccgcca
     9601 cgtcatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc tgccaaacgc
     9661 tccctgccag tcctgctcaa acttcgccac agcggcttcc accgccggat agctaatcat
     9721 ctgttgtgcc acatccagtg gcagagtaat tgattcacat ccggccagta agcagtccag
     9781 cgcctgacgc ggggttttga aactcgctgc cagcactttc gcctgcggcg catgcatttt
     9841 caataactgg tgtaagtcgg tcacagtctg aatgccgcta ccgccctgag catcaatacg
     9901 attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagcagcc cttgtgccgc
     9961 gccatatacc gcggttccca gcgtcggaat cccttccgct tttaacatct taatcgctgc
    10021 cagcccctcg gctgtcaccg gaactttcac cacgatatcc gcaataatag aacgcagctt
    10081 aagcgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa acagacgccc
    10141 ctgaccgccc atcgcttcat gaagttgcgg aagcacaaca tccagcggtt ttttacccgc
    10201 ggcgataatg cttgggttag tggtcacacc cgccagcgga aaaatacgtg acagcgcctt
    10261 caccgcaaca acgtctgaag tatccagata cagttccata accatcctca aaatgtactt
    10321 tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca catcaatcta
    10381 actttcattc gaaagtattt taatgtttga acgaaacaga gaggcaacta tgattttcaa
    10441 tattcagcgc tactcgaccc atgatggccc cggtatccgc acggtcgtat ttcttaaagg
    10501 ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgta cgcaggatct
    10561 gctgtatgac gcacgactgt gtctggaagg ctgcgagctg tgcgctaagg ccgcgccgga
    10621 agtgattgag cgcgcgctga atggtttgct tattcatcgg gaaaagttaa ccccggagca
    10681 tctgacggcg ttaaccgact gctgtccgac acaggcgtta accgtgtgtg gtgaagtgaa
    10741 aagcgttgag gagatcatgg cgaccgttct gcgcgataaa ccgttttacg atcgcagcgg
    10801 cggcggctta acgctttcgg gtggtgaacc cttcatgcag ccggaaatgg cgacggcgct
    10861 gctgcaagcc tgccacgagg caggcattca tactgcggta gaaacctgtc tgcatgtgcc
    10921 gtggaaatat atcgcccctt ctctgcccta tatcgatctg tttcttgccg atttaaaaca
    10981 cgttgccgac gcgccgttta aacagtggac cgacggtaac gccgccaggg tgctggataa
    11041 cctgaaaaaa ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccgc tgattcaggg
    11101 ctttaatgcc gacgaaacct ctgtaaaagc cattaccgat tttgccgccg acgagctgca
    11161 cgttggcgaa attcattttc tgccctacca cacgctgggc atcaacaaat atcacttact
    11221 taatctgccc tatgacgccc cggaaaaacc gcttgatgcg ccagagctgc tcgattttgc
    11281 ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac aaccatgacc
    11341 acactgaaac tggacacgct cagcgaccgc attaaagcgc acaaaaatgc gctggtgcat
    11401 attgtgaaac cgccagtctg taccgagcgc gcgcagcact ataccgagat gtatcaacaa
    11461 catctcgata agccgatccc ggtacgtcgc gcgctggcac tggcgcatca cctggcgaat
    11521 cgcaccatct ggatcaaaca ctatgagttg atcattggca accaggcaag cgaagttcgc
    11581 gccgcgccga tcttcccgga atatactgtc tcgtggatcg aaaaagagat tgatgatctg
    11641 gcagatcgtc ccggtgctgg ctttgcggtg agcgaagaga acaaacgtgt tctgcatgaa
    11701 gtgtgcccgt ggtggcgcgg tcagacggta caggatcgct gctatggcat gtttaccgat
    11761 gagcaaaaag gtctgctggc gaccggcatc attaaagcgg aaggcaatat gacctccggc
    11821 gatgcgcacc tggcggtcaa tttcccgctg ctgctggaaa aagggcttga tggtctgcgt
    11881 gagaaagtag cggaacgtcg ctcgcgcatc aatctgacgg tgctggaaga tttgcacggc
    11941 gaacagttcc tgaaagcgat tgatatcgtg ctggtggcag tcagtgaaca cattgaacgt
    12001 ttcgctgccc tggcgcgtgc aatggccgcg accgaaaccc gcgaaagccg tcgcgatgaa
    12061 ctgctggcga tggcagaaaa ctgcgatctt atcgcccacc agccgccgca gactttctgg
    12121 caggcgctgc aactgtgtta cttcatccag ttgattttgc agattgaatc taacggtcac
    12181 tcggtgtcgt ttggtcgtat ggatcagtat ctctacccgt actatcgccg cgacgttgaa
    12241 ctgaaccaga cgctggatcg cgaacacgcc atcgaattgc tgcatagctg ctggttgaaa
    12301 ttgctggaag tgaacaaaat ccgctccggc tcccactcaa aagcctctgc gggaagtccg
    12361 ctgtatcaga acgtcaccat tggcggacaa aatctggttg atggtcaacc aatggacgcg
    12421 gtgaatccac tctcttacgc gatcctcgaa tcctgcggtc gcctacgttc cactcagcct
    12481 aacctcagcg tgcgttacca tgcaggaatg agtaacgatt tcctcgacgc ctgcgtacag
    12541 gtgatccgct gcggcttcgg gatgccggca ttcaacaacg acgaaatcgt gatcccggaa
    12601 tttattaaac tcggtattga accgcaggac gcttatgact acgcagcgat tggttgtata
    12661 gaaaccgccg tcggtggcaa atggggctat cgctgtaccg gcatgagctt tatcaacttc
    12721 gcccgcgtga tgctggcggc gctggaaggc gggcgtgatg ccaccagcgg caaagtgttc
    12781 ctgccacaag aaaaagcgtt gtcggcaggt aacttcaaca acttcgatga agtgatggcc
    12841 gcgtgggata cgcaaatccg ttactacacg cgtaaatcaa tcgaaatcga atatgtcgtc
    12901 gacaccatgc tggaagagaa cgtgcacgat attctctgct cggcgctggt ggatgactgt
    12961 attgagcgag cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt ttctggcctg
    13021 caggtcggta ttgccaacct cggcaacagc ctggcggcag tgaagaaact ggtgtttgaa
    13081 cagggcgcga ttggtcagca acagctggct gccgcactgg cggatgactt cgatggcctg
    13141 actcatgagc aactgcgtca gcggctgatt aacggtgcgc cgaagtacgg caacgacgat
    13201 gataccgtcg atacgctgct ggctcgcgct tatcagacct atatcgacga actgaaacag
    13261 taccataacc cacgctacgg tcgtggtccg gttggcggca actattacgc gggtacgtcg
    13321 tctatctccg ctaacgtacc gtttggcgcg caaactatgg caacgccgga cgggcgtaaa
    13381 gcccacaccc cgctggcaga aggcgctagt ccggcctctg gtactgacca tctcggccct
    13441 actgcggtca ttggctcagt gggtaaactg cctacggcag cgattctcgg cggcgtgttg
    13501 ctcaaccaga aactgaatcc ggcaacgctg gagaacgaat ctgacaagca gaaactgatg
    13561 atcctgctgc gtaccttctt tgaagtgcat aaaggctggc atattcagta caacatcgtt
    13621 tcccgcgaaa cgctgctgga ggcgaaaaaa catccggatc agtaccgcga tctggtagtg
    13681 cgtgtcgcgg gttattccgc cttcttcacc gcgctctctc cagacgctca ggacgatatc
    13741 atcgcccgta ctgaacatat gctgtaatct cttcggcccg gctgcggcgt cgccgggcta
    13801 aatcattcac atcatcaatt tcatccttac tttcattcga aatataattt gtgctctgcg
    13861 tcacattgtt attacactgt tacggtttca ttttcgagcc aggagcacac ctatgagcgt
    13921 aaaagttatc gtcacagata tggacggtac ttttcttaac gacgccaaaa cgtacaacca
    13981 accacgtttt atggcgcaat atcaggaact gaaaaagcgc ggcattaagt tcgttgtcgc
    14041 cagcggtaat cagtattacc agcttatttc gttctttcct gagctaaagg atgagatctc
    14101 ttttgtcgcg gaaaacggcg cactggttta cgaacatggc aagcaactgt tccacggcga
    14161 actgacccga catgaatcgc ggattgttat tggcgagttg ctaaaagata agcaactcaa
    14221 ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa aatgcccccg aagcatttgt
    14281 cgcactgatg gcaaaacact accatcgcct gaaacctgta aaagattatc aggagattga
    14341 cgacgtactg ttcaagtttt cgctcaacct gccggatgaa caaatcccgt tagtgatcga
    14401 caaactacac atagcgctgg atggcattat gaaacccgtc accagtggtt ttggctttat
    14461 cgacctgatt attcccggtc tacataaagc aaacggtatt tcgcggttac tgaaacgctg
    14521 ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg cggagatgct
    14581 gaaaatggcg cgttattcct ttgcgatggg caatgctgcg gaaaacatta aacaaatcgc
    14641 ccgttacgct accgatgata ataatcatga aggcgcgctg aatgtgattc aggctgtgct
    14701 ggataacaca tcccctttta acagctgacc tctctaccgg agccatgcgc tccggttttc
    14761 ccttcttttt acatcctgta catcacatca aatcattaaa cttgcattaa cttatttata
    14821 acttgtattg tcacatgcaa atcagcatag tttcgaatga aagttaatgc gaggtggtta
    14881 tggcttctac ttttaccagc gacacattgc ctgccgatca caaagcagct atccgtcaga
    14941 tgaagcacgc gctgcgggcg cagcttggcg atgtccagca gatctttaat cagctaagcg
    15001 atgacattgc cacgcgagtg gctgaaatca acgcactcaa agcacagggc gatgccgtct
    15061 ggccggtgct gtcttatgcc gatatcaaag caggtcatgt tactgcagag cagcgcgaac
    15121 agattaaacg tcgcggttgt gcggtgataa aaggccattt cccccgcgaa caagcgctag
    15181 gctgggatca gtcgatgctg gactatctga accgcaaccg ctttgacgag gtctacaaag
    15241 gccccggcga taatttcttc gggacgctca gcgcttcacg tcccgagatt taccccatct
    15301 actggtcgca ggcgcaaatg caggcccgcc agagtgaaga aatggcgaat gcgcagtcgt
    15361 ttctcaatcg tctgtggaca tttgaaagtg atggaaagca atggtttaac ccggatgtga
    15421 gcgtcatcta ccctgaccgt atccgccgcc ttccgcccgg aacgacctcc aaaggtcttg
    15481 gagcgcatac cgactccggg gcgctggaac gctggctgct tccagcgtat cagcacgttt
    15541 tcgccaacgt ctttaatggc aatctggcga aatacgatcc ctggcatgcg gcacatcgta
    15601 cggaagttga agagtacacg gtggacaaca ccaccaaatg ttccgtgttt cggacattcc
    15661 agggctggac tgcgctctcg gatatgctgc ctggtcaggg gttgctgcac gtcgtgccca
    15721 ttcctgaagc catggcgtac gtactgttac gtccgctgct tgatgatgtg ccggaggatg
    15781 aactgtgcgg cgtagcgccc ggaagagtgt tgccggtatc agagcaatgg catccactgt
    15841 taattgaggc gttaaccagc attccaaaac tcgaggccgg agactccgtc tggtggcact
    15901 gcgacgtcat ccattccgtt gcccccgttg aaaatcaaca gggttggggc aacgtgatgt
    15961 acattcctgc ggcaccgatg tgcgagaaaa atcttgccta cgcgcacaag gtgaaggccg
    16021 cactggaaaa aggcgcatcg ccgggcgact tcccgcgcga ggactatgaa acaaactggg
    16081 aaggacgctt tacgcttgcc gacctcaaca ttcacggtaa gcgagcgttg ggcatggatg
    16141 tttgagagtt aatggaagtg aattgatgtg ggattaagaa aatcgattcg acgttactca
    16201 gattgctgac aagtgcgctt tcttaatgcc tgatgcggcg ttaacgcctt atccggccta
    16261 caaagttctg cagattcaat atattacagg tactgcgtag gcctgataag cgtagcgcat
    16321 caggctattt tgcatttgtc atcagccgtc gtcaccccga cagtatcggg gtgacggata
    16381 atcacttact cgatcccttt actacgcagg taatcttcgt aattaccgct aaagtcgatc
    16441 acgcattccg gggtgatttc cagaatgcgg gtcgccaggg agcttacgaa ctcacggtcg
    16501 tgtgaaacaa agatcagcgt gccctgatac agttccagcg ccatgttcag tgactcgatg
    16561 gattccatat ccaggtggtt ggtcggttcg tccatgatca gaatgttcgg cttctgcatc
    16621 attaacttac caaacagcat ccgccctttt tccccaccgg aaagcacttt agctggcttt
    16681 ttgatgtcgt cctggctgaa cagcaaacga ccgagaatgc tgcgtaccgc ctgctcgtca
    16741 tcgccttcct gcttccactg actcatccat tcgaacacgg tcagatcatt ttcaaactca
    16801 tattcgtggt cctgggcgta gtaaccaatg cgcgcgtttt cagaccattt tacggtgccg
    16861 ctgtccggtt gcagatcgcc caccagcgtt ttcagcagcg ttgatttacc gacgccgttg
    16921 gtacccagta ccgcaagttt ttcaccgacc tccagcagca ggttgagatt tttaaacagt
    16981 ggaccgttat caaacccttt ggtcagacct tccacttcca gcgcgttacg gaacagtttc
    17041 ttatcctgtt caaaacggat gaacgggttc tgacggctgg aggctttcac ctcttccagt
    17101 ttgattttat caatctggcg cgcgcgcgaa gttgcctggc gagatttcga ggcgttggcg
    17161 ctaaagcggc taacgaaaga ttgcaactca gcaatctgcg ctttcttctt ggcgttatcg
    17221 gccagcagac gttcacgcgc ctgggtcgcc gccgtcatgt actcatcgta gttacccgga
    17281 taaacgcgca gctcgccgta atccagatcc gccatgtggg tacagaccat gttaaggaag
    17341 tgacggtcgt gcgagatgat gatcatggtg ctgtcacgct cgttcagcac ctgttccagc
    17401 cagcgaatgg tgtcgatgtc gaggttgttg gtcggttcgt cgagcaggag aatatccggc
    17461 tcagcaaaca gcgcctgcgc cagaagcaca cgcagcttcc agccaggagc aacttcactc
    17521 atcgggccgt agtgctgttc cactggaatt cccacgccaa gcaacagttc accggcgcga
    17581 gcttccgcag agtaaccgtc catttcgccg tatttcactt ccagatcggc cactttatag
    17641 ccgtcttctt cactcatttc cggcaaagca tagatgcggt cgcgctcctg cttcacttcc
    17701 cacaactctt tatgccccat gatcaccgta tccagcacag tgaacccttc aaaggcaaac
    17761 tgatcctgac gcagcttacc aatgcgctcg ttgggatcga gggaaacgtt acccagcgtc
    17821 ggctcaaggt cgccgccgag aatcttcata aaggtggatt taccactacc gttcgcgcca
    17881 atcaggccgt aacggttgcc gccgccgaat ttgacggaaa tgttttcaaa caacggctta
    17941 ctgccgaact gcatggtgac gttactggaa actaacacag gcgtatcctg aaaagagata
    18001 tgacaaaccg cgcattatgc cataagtctg aaagaagatc ctgctcctcg cttaaggtct
    18061 acactcaagc caaaggcaaa aaatgtgatt tcgtacacat ctgatttcac tgtgagctgg
    18121 aatgaactta taatgcgctt ccaatactct aatattctca acccaatggc ctgccaggca
    18181 caaaatctcg cttaacatga atatgaaatt gaaaacatta ttcgcagcgg ccttcgctgt
    18241 tgtcggcttt tgcagtaccg cctctgcggt aacttatcct ctgccaaccg acgggagtcg
    18301 cctggttggt cagaatcagg tgatcaccat tcctgaaggt aacactcagc cgctggagta
    18361 ttttgccgcg gagtaccaga tggggctttc caatatgatg gaagcgaacc cgggtgtgga
    18421 taccttcctg ccgaaaggcg gtactgtact gaacattccg cagcagctga tcctgccgga
    18481 taccgttcat gaaggcatcg tcattaacag tgcagagatg cgtctgtatt actatccgaa
    18541 agggaccaac accgttatcg tgctgccgat cggcattggt cagttaggca aagatacgcc
    18601 tatcaactgg accaccaaag ttgagcataa gaaagcaggc ccgacctgga cgccgaccgc
    18661 caaaatgcac gcagagtacc gcgctgcggg cgaaccgcta ccggctgtcg ttccggcagg
    18721 tccggataac ccgatgggtc tgtatgcact ctacatcggt cgcctgtatg ctatccatgg
    18781 caccaacgcc aacttcggta tcggcctgcg tgtaagtcat ggttgtgtgc gtctacgtaa
    18841 cgaagacatc aaattcctgt tcgagaaagt accggtcggt acccgcgtac agtttattga
    18901 tgagccggta aaagcgacca ccgagccaga cggcagccgt tatattgaag tccataaccc
    18961 gctgtctacc accgaagccc agtttgaagg tcaggaaatc gtgccaatta ccctgacgaa
    19021 gagcgtgcag acagtgaccg gtcagccaga tgttgaccag gttgttcttg atgaagcgat
    19081 caaaaatcgt tccgggatgc cggttcgcct gaattaatcg tcaaaacgta aaacaaaggc
    19141 ggactcataa tccgcctttt ttatttgcca gaccttacca ggccgggatc ataactaaca
    19201 aaacgtatcc caccagcgcc gcccacaaca gcatcggtat tgaatagaga tggaaacgcc
    19261 accagatgcg gcgatcgttc gccatacgta gcgcaatcaa atttgccagc gatcctggta
    19321 ataaaccaaa gccccccacg tttaccgccc atgccagtaa taaagacgga ggtacataat
    19381 tgagcagcaa aatggtactc ggcacgttac tgatcacctg cgataaaccg actgccgtta
    19441 accataaccc gggttcagat aaatgactca cgttacccaa cacgccttgc aacgctggaa
    19501 gctgggtcag taaatggacg tcgataaaca tcgccataaa caccagcagc aacgtccagt
    19561 ccacactcaa caccacgcga cgcgccagca gcacaaagcc cgccgccaca atcaccagtc
    19621 cccacagctc ttgtttgaac tccagcgccg tcagaaaaac gatatacagc cccagacaat
    19681 tccacaccag ccgcggtttc cactccggtg tttgcatccc cgtatggtat tgcagcgcct
    19741 ttccagggaa acaacaccag cacaacagca ggagtgtcag catcattgcg ccagccagcg
    19801 gtgccatttg ggcaataaat ccggcaaacg aaagaccaga acgtccccag ataagaatat
    19861 tttgcgggtt gccaattggc gtcagtagcg aaccagcgtt gactgccagc gcctcaaaaa
    19921 taatcagccg attaaccggg atctcacaca gtctttttag cgtgatagtc agcggaacaa
    19981 caataaacag cgcgacatcg ttggtcagaa aggtagaaag cagcgccgcc gccagcacca
    20041 taaacatcgc cagccgacgc tccgtagcaa agcggcgcac cattttgcgc cccagcacat
    20101 caaaataacc gcttaactcc acacctttgg tcagcagcat caggccgctt aaggtgatga
    20161 tggtgtgcca gtcgatagca gcaggccagg atttcggtgc aaagggcacg aaaaagctta
    20221 atccgatacc aacaagaatt aataactgaa aaaaacgatc gccttgcagc gtgcgtaaaa
    20281 aaggcaggct catttggcac cgtgtttctg ggtaaacaaa cgaaaagcgt cgagcgtctc
    20341 ttcactaaca tggtgctcca tgccttccgc gtcgcgacgg gcgatttccg gactgacgcc
    20401 caacaccaac aagaaatttt cgactatctg atgtcgctcg cggctttcct gcgccagctt
    20461 ctctccttct gccgttaaaa acacgccacg ccaggggatc atttcaatca gccccatggt
    20521 tgccagccgc ttaagcattt tagccaccgt cggttgcgaa actcccagac gagcagccat
    20581 gtccacctga cgagcttccc ccacttccct gatcaagtca gaaatcagct caacgtaatc
    20641 atcaataagc tcgcgccgat gcgcctctct gacctggcgg aacccttcaa cgtgctcttc
    20701 cacgttcact aactgcgtca ctttttttgc tgttggcgta cctgcgcgac gactcattgt
    20761 gcttcctcat tatggtgacg catacatagc gttctcattt cgtgcgcaca ttgtaaacca
    20821 gagttgcgaa ggtacaaaaa attaacgttt tagcaatagc tatataatat agcctgtgct
    20881 atatctgtat gtaatgcaat catccctcaa ggatcgacgg gattagcaag tcaggaggtc
    20941 ttatgaatga gttcaagagg tgtatgcgcg tgtttagtca ttctcccttt aaagtacggt
    21001 taatgctgct ctctatgttg tgcgatatgg tcaacaacaa accgcagcaa gataaacctt
    21061 cagataaata gcggcgtcgc ggtacgccgc ttcactcctg cttttatgca ggcataacgc
    21121 gttttggtct gaaaaaaacc actttttgtc ggatttgcaa tccccttcgc aaaagatttg
    21181 ttcgtcagta gttgacctga acggcggctc gctctatctt cttgcagccc tgcgtatatt
    21241 gcggctcgcg gatgcggacc cctttcccct cttcacgcac tcttgcaggt attgaccctt
    21301 gacgccaggg taagcacatg gcgtttgtta ccatagtggc atatgaattt aaccctcaaa
    21361 gaatcgcttg ttacccgtag ccgggtattt agcccgtgga ctgcgttcta ctttttacag
    21421 tcgctattaa ttaacctcgg cttaggttac cccttcagtt tgctctacac cgctgcgttt
    21481 acggctattt tgcttttgct atggcgaaca ttgcctcgcg tacaaaaagt tctggtcggt
    21541 gtcagttcgc tggtggcggc ttgttatttc ccttttgctc aggcctacgg cgcgcctaat
    21601 ttcaatacat tgctggcatt gcactccacc aatatggaag agtcgaccga aatcctgacg
    21661 atttttccgt ggtacagcta cctggtcggc ttatttattt ttgcgctcgg cgtaatagca
    21721 atcaggcgaa aaaaagagaa tgaaaaagcg cgctggaata ccttcgacag cctgtgtctg
    21781 gtattcagtg tggcgacatt ttttgttgct cccgtgcaaa acctggcctg gggtggcgta
    21841 tttaaactga aagatactgg ctatccggta tttcgttttg ctaaggatgt catcgtcaat
    21901 aataacgagg tgattgaaga gcaagaacgg atggcaaaac tttccggaat gaaagatacc
    21961 tggacggtca ctgccgttaa gccgaagtat cagacctatg tggtagtgat cggtgaaagc
    22021 gcgcgtcgcg atgccctcgg tgcctttggc ggtcactggg acaatacccc gtttgccagc
    22081 agcgttaacg gtttgatatt tgctgactac attgccgcca gtggctccac gcagaaatcg
    22141 cttggcttaa cgctcaatcg cgtagtcgat ggcaaaccac agtttcagga taactttgtc
    22201 accctggcaa atcgcgcggg cttccagacc tggtggtttt ccaaccaggg ccaaatcggc
    22261 gaatacgata ctgctatcgc cagcatcgcc aaacgtgcag atgaagtgta cttcctgaaa
    22321 gaaggtaatt ttgaagcaga taaaaacacg aaagacgaag cgttactgga tatgaccgct
    22381 caagtgctgg cgcaagagca ctcgcaaccg cagctgattg ttctgcatct gatgggctcg
    22441 catccgcagg cctgcgacag gacacaaggc aaatacgaaa cctttgtgca atcgaaagaa
    22501 acgtcgtgct atctctatac catgacgcaa acggacgatt tactgcgcaa gctgtacgat
    22561 cagttacgca acagcggcag cagcttctct ctggtttact tttctgacca cggtctggcc
    22621 tttaaagagc gcggcaaaga cgtgcaatac cttgcccatg atgataaata tcagcaaaat
    22681 ttccaggtgc cttttatggt catttccagc ggcgataaag cgcatcgggt gattaaagcc
    22741 cgccgctcag ccaatgactt cttaggcttt ttctcgcagt ggacgggaat taaagcgaag
    22801 gaaataaata tcaaataccc gtttatatct gagaagaaag ccgggccgat atacatcacc
    22861 aacttccagt tacagaaggt ggattacaac catctcggaa ccgatatttt cgacccgaaa
    22921 ccttaaaaac aaaaatccgc cccgagaggc ggatttttat atcaccaaag tgattagaag
    22981 cggtaaccaa caccggcaat ccaggtgcct acgtcaacgc tacgaatacg gctctgctcg
    23041 taagagaagt ccagagcaac gttttccatc gggttgaact gcagaccagc accgtaggag
    23101 aaaccgtagt cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg gaatttacca
    23161 taacccacac ccactacacc gtagatgctt gcccagtcgt taatgcggta agccggacca
    23221 gcagtgatgc cgtagtactg gtttttgttg tagtcaccag agcttgcagt acggcttttc
    23281 tcggtgtaag tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc atagcggtat
    23341 ttcaggttga aaccgcccat tttgttcatt tggccctgag cgtcgctctg tgcgtaaccg
    23401 ccagttacgg tagaagtcgc agctacggaa gtgcctgcgg tgaaagccag aactgcggcc
    23461 agtgctgaaa gacatgcaat ttttttcata accacctcaa atgtgattca aataagtcct
    23521 aagttttaaa tatatcaaaa attaatggga aactcttcgc gatttgtgat gtctaacggg
    23581 ccatttcatg taacagaacg tttccataca ccgctatcca tctaaattta aatcactttt
    23641 tcagagaact gcgtaagtat tacgcatgtt ttccctgtca ttcatccaga ttattcctaa
    23701 tcaccagact aatgattcca tcaatcctgg cgcattttag tcaaaacggg ggaaaatttt
    23761 ttcaacaaat gctcaaccag cattgggtat atccagtaca ctccacgctt tacttaagtc
    23821 tagatatttg tgggagaaag gatgcctggt tcattacgta aaatgccggt ctggttacca
    23881 atagtcatat tactcgttgc catggcgtct attcagggtg gagcctcgtt agctaagtca
    23941 ctttttcctc tggtgggcgc accgggtgtc actgcgctgc gtctggcatt aggaacgctg
    24001 atcctcatcg cgttctttaa gccatggcga ctgcgctttg ccaaagagca acggttaccg
    24061 ctgttgtttt acggcgtttc gctgggtggg atgaattatc ttttttatct ttctattcag
    24121 acagtaccgc tgggtattgc ggtggcgctg gagttcaccg gaccactggc ggtggcgctg
    24181 ttctcttctc gtcgcccggt agatttcgtc tgggttgtgc tggcggttct tggtctgtgg
    24241 ttcctgctac cgctggggca agacgtttcc catgtcgatt taaccggctg tgcgctggca
    24301 ctgggggccg gggcttgttg ggctatttac attttaagtg ggcaacgcgc aggagcggaa
    24361 catggccctg cgacggtggc aattggttcg ttgattgcag cgttaatttt cgtgccaatt
    24421 ggagcgcttc aggctggtga agcactctgg cactggtcgg ttattccatt gggtctggct
    24481 gtcgctattc tctcgaccgc tctgccttat tcgctggaaa tgattgccct cacccgtttg
    24541 ccaacacgga catttggtac gctgatgagc atggaaccgg cgctggctgc cgtttccggg
    24601 atgattttcc tcggagaaac actgacaccc atacagctgc tggcgctcgg cgctatcatc
    24661 gccgcttcaa tggggtctac gctgacagta cgcaaagaga gcaaaataaa agaattagac
    24721 attaattaaa tttacatttc tgtatggtta tgcataacca tgcagaattt ctcgctactt
    24781 ttcctctaca ccgtctttat atatcgaatt atgcaaaagc atatttattc cgaaaattcc
    24841 tggcgagcag ataaataaga attgttctta tcaatatatc taactcattg aatctttatt
    24901 agttttgttt ttcacgcttg ttaccactat tagtgtgata ggaacagcca gaatagcgga
    24961 atacatagcc ggtgctatac ttagtctcgt taattactgg gacataacat caagaggata
    25021 tgaaattatg agtaccgcta aattagttaa atcaaaagcg accaatctgc tttatacccg
    25081 caacgatgtc tccgacagcg agaaaaaagc aacagtagag ttgctgaatc gccaggttat
    25141 ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc gcggcgctaa
    25201 cttcattgcc gtacatgaaa tgctggatgg cttccgcacc gcactgatcg atcatctgga
    25261 taccatggca gaacgtgccg tgcagctggg cggtgtagct ctggggacca ctcaagttat
    25321 caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc aggatcacct
    25381 gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag cgattggcga
    25441 agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc tggataaatt
    25501 cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca gaactttagt
    25561 acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagccca cctcaacgca
    25621 cttatttagt gcatccatct gctatcccca gctgattaag taaatttttt gtatccacat
    25681 catcacacaa ccgttacata aagattgttt tttcatcagg ttttacgcta aataatcact
    25741 gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc agaatggtgc atcttcaggg
    25801 tattgctcta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca atgagttgca
    25861 aaactggcac gattttttca tatatgtgaa tgtcacgcag gggatcgtcc cgtggataga
    25921 aaaaaggaaa tgctatgaag tctgtattaa aagtttcact ggctgcactg accctggctt
    25981 ttgcggtttc ttctcatgcc gcggataaaa aattagttgt cgcgacggat accgccttcg
    26041 ttccgtttga atttaaacag ggcgataaat atgtgggctt tgacgttgat ctgtgggctg
    26101 ccatcgctaa agagctgaag ctggattacg aactgaagcc gatggatttc agtgggatca
    26161 ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc atcaccgacg
    26221 agcgtaaaaa agcgatcgat ttctctgacg gctactacaa aagcggcctg ttagtgatgg
    26281 tgaaagctaa caataacgat gtgaaaagcg tgaaagatct cgacgggaaa gtggttgctg
    26341 tgaagagcgg tactggctcc gttgattacg cgaaagcaaa catcaaaact aaagatctgc
    26401 gtcagttccc gaacatcgat aacgcctata tggaactggg caccaaccgc gcagacgccg
    26461 ttctgcacga tacgccaaac attctgtact tcatcaaaac cgccggtaac ggtcagttca
    26521 aagcggtagg tgactctctg gaagcgcagc aatacggtat tgcgttcccg aaaggtagcg
    26581 acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac ggaacttaca
    26641 acgaagtcta caaaaaatgg ttcagtactg aaccgaaata ataacgctac acctgtaaag
    26701 cgcactggca gttccctctc ccccctgggg agaggattag ggtgaggggc gcaaacccgc
    26761 tccggggcca ttaattaccc tgaatttgat tatttacacc acggtaacag gaacaacata
    26821 tgcagtttga ctggagtgcc atctggcctg ccattccgct tctgattgaa ggtgccaaaa
    26881 tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga ttgctggcag
    26941 gttttgcacg caccttcggt ggttggatag ccaaccacgt cgcgctggtc tttattgaag
    27001 tgatccgcgg cacacctatc gtcgtccagg tgatgtttat ctatttcgcc ctgccgatgg
    27061 cgtttaacga cttacgcatc gacccattta ctgcggcggt ggtcaccatc atgatcaact
    27121 ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct gtctatccac aaaggttttc
    27181 gtgaagcagg actggcgctc ggtctttcac gttgggaaac cattcgctac gtcattttac
    27241 cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc agcattaaag
    27301 acacctcact gtttattgtt atcggcgtgg cggaactgac ccgtcagggg caagaaatta
    27361 ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc tatctgatta
    27421 ttaccctggt gctgagcttt attctgcgtc gtctggaaag aaggatgaaa atcctgtgat
    27481 tgaatttaaa aacgtctcca agcactttgg cccaacccag gtgctgcaca atatcgattt
    27541 gaacattgcc cagggcgaag tcgtggtgat tatcgggccg tccggttccg gtaaatcgac
    27601 cctgctgcgc tgcatcaaca aactggaaga aatcacctcc ggcgatctga ttgtcgatgg
    27661 cctgaaggtt aacgatccga aagttgacga gcgcctgatt cgccaggaag caggtatggt
    27721 gttccagcag ttttacctct tcccgcatct gacggcgctg ggaaacgtca tgtttggtcc
    27781 gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa ctggcgcgtg agctgctggc
    27841 gaaagtcggt ctggcagaac gtgcacatca ctacccttcc gaactttctg gtggtcaaca
    27901 gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc tgtttgatga
    27961 accgacttcc gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta tgcaggatct
    28021 ggctgaagaa gggatgacga tggtgatcgt gacccacgaa atcggttttg ccgagaaagt
    28081 agcttcgcgg ctgatcttta tcgacaaagg ccggattgcg gaagatggca atccgcaggt
    28141 gttgatcaag aacccgccga gccagcgctt gcaggaattt ttgcagcacg tctcttaata
    28201 agacacattg cctgatgcgc tacgcttatc aggcctacag gatatctggc aacttattaa
    28261 aattgcatga acttgtagga cggataaggc gttcacgccg catccggcaa aaaagcccgc
    28321 acgttgtcag caacctgctt aatatccctt cctccctttc acccgaaagg gaggcacacc
    28381 agattcctct catttaaaat cgcccctccc ccagcatcta tacttatctt tttgctctgt
    28441 tttctcactg gaggagtcat gcggtggatc ctgttcatcc tcttctgcct gctgggcgca
    28501 cctgcccacg cggtatccat acccggcgtt acaaccacaa cgacaacgga ctcaacgact
    28561 gaaccggccc cggaaccgga tatcgaacaa aaaaagcggc ctatggcgca ctggcggatg
    28621 tgctggataa cgacacctcg cgtaaagagt tgatcgacca gttgcgtact gtcgccgcga
    28681 cgccccctgc ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa gagcaaaccg
    28741 tgctgcaaaa ggtcaccgaa gtcagccgcc attatggtga agccctttcc gcccgcttcg
    28801 ggcaacttta tcgcaatatc accggctccc cgcataagcc atttaatcca caaaccttca
    28861 gcaatgcgct gagccatttt tcaatgttag cggtattagt gtttggtttt tactggctga
    28921 ttcgcctgtg cgcactgccg ctatatcgca aaatgggtca gtgggcgagg caaaaaaatc
    28981 gtgagcgcag caactggttg cagcttccgg cgatgattat tggggcgttt attatcgacc
    29041 tgctgttact ggccctgaca ttatttgtcg gccaggtatt aagcgacaac ctgaatgcag
    29101 gtagtcgcac catcgctttc caacaaagtt tgtttctcaa cgcctttgcc ctgattgaat
    29161 ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt ggcggagctg cgcccgttca
    29221 cgattcagga cgagagcgcc cgttactgga gtcgccgcct gagctggtta agcagcctga
    29281 ttggctatgg cctgattgtg gccgtgccga ttatctctaa tcaggtgaat gtacagatag
    29341 gtgcgctggc gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg tacctgatct
    29401 tccgaaataa aaaagagatt acccagcatt tgctcaactt tgcggagcat tcgctggctt
    29461 ttttcagcct gtttatccgc gcctttgcgc tggtgtggca ctggctggca agcgcctatt
    29521 ttatcgtgct gtttttcttt tcgttgttcg atccgggcaa cagtctgaaa tttatgatgg
    29581 gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc ggtatgtttt
    29641 cccgctggct ggcgaaaacc atcaccctct cgccacatac tcagcgtaac tatccggagc
    29701 tacaaaaacg gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt attctgaccg
    29761 tctgcgtggc agtaatgttg ctgttgagcg catggggatt gttcgatttc tggaactggc
    29821 tgcaaaacgg cgcggggcag aaaaccgtag atatcctgat ccgcatcgca ctcattcttt
    29881 tcttctcggc ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg ctggcttcgg
    29941 atattcatgg ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg ctgttccgta
    30001 acgcgctggc ggtgattatc agtaccatca ccatcatgat tgtgttgtcg gaaatcggcg
    30061 tcaatatcgc gccattgctg gcaggtgccg gggcattagg tctggctatc tcgtttggtt
    30121 cgcaaacgct ggtaaaagat attatcaccg gggtatttat tcagtttgaa aacggcatga
    30181 acactggaga tttggtgact atcgggccgt tgaccggcac agtggaacgg atgtcgattc
    30241 gctccgtggg cgtgcgccag gataccgggg cgtatcacat cattccgtgg tcttcgataa
    30301 ccacctttgc taacttcgtc cgcggtattg gttcggtagt ggcaaactac gatgttgatc
    30361 gccatgaaga tgctgataaa gccaatcagg cactgaaaga tgcggtaacg gaattgatgg
    30421 aaaacgaaga aattcgcggg ctgattattg gtgaaccgaa ctttgccggg attgtcggct
    30481 taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc aaacagtgga
    30541 cggtacgctt tgccctcgac agccaggtga aaaaacattt cgacctggcg ggcgttcgcg
    30601 cgccagtgca gacttatcag gtgctgcctg ctccgggcgc gaccccggct gaaccgctgc
    30661 cgccggggga gccaacgctt taacgttggc gattaacaaa acggcggcgc tgctcgtcgt
    30721 tcataaaggt ccaggcaata aagcgactct gcttttgccc ctgggccatc tcttttttta
    30781 ccaccttcac cgcgcccacg tcggtcagag cacgatacaa cggcggtaag ttttcaccac
    30841 gagataccag cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc tcttcaatca
    30901 tctttttgat aaagttgact tcaccgcctt cacaccacaa ctcctgttgc tgaccaccaa
    30961 agttcagtgc atcgtctttg ttcagcccca ggttacggcg tttacgctca ctacctgccc
    31021 gcgccgcagc ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg tattgctcgt
    31081 ttttgtggat gatgccgtta aaaatcgccc cactctcttt ttgccgacgc agacgaatgg
    31141 cgcggttaag ccccggatta gcactgataa tcgcctgcgc actgcttaac gcctggctgc
    31201 tggtttcact accggtaaaa cgccagccat attcatgtac gccaatcagc ggataaatac
    31261 agttcgcacc aacgccgata tccagaatgc tggcattcgc tggaattgtt ccgcttgctt
    31321 cagccagtaa atcggcaagg tgatgaatat aatccgcccg gcccggtact ggtgggcaga
    31381 gaaaaccgtc ggggatatcc cagttcgcta cggcgtaaaa atgggccagc aacgccttat
    31441 tgagcgcctt caccgccagc ggattggcaa agtctacgct ttgctcccct gcgggtgtaa
    31501 gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag cgtggcgaga tcgtagcggc
    31561 tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc ggacatagca ttctccttta
    31621 agcagcggcg taagataccc gcataacgcg acagggtaaa tatctggctg cgtttatcat
    31681 tccccaaaac cttaacatgg ctatggttta tggcaattca tcataaaaat cagcattatg
    31741 catgtcattg atttgtatta ttttgtaaaa atatatcgtt caaaaaataa gcatatctct
    31801 gtcaattttt tgcgtcattt gcatcgcgca ggcgacctac acttatcagg cactacccgt
    31861 tgtatttatg aggatgtcat tatgaaaaag tgcctcacac tactgattgc caccgtcctg
    31921 agcggaatct ctctcacggc ttatgccgcg caaccgatga gtaacctgga cagcggtcag
    31981 ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga tctggaggat
    32041 aaactggcag aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa ttccgcaggc
    32101 ggaaatgacc agatgttcgg tactgcaacc atctacaaat aaccccacca acgtaactgc
    32161 attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa atatgaacgc
    32221 aatgtaaata aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc gtcaagtgat
    32281 aatgcttatc aaaattatta tcacttgcac aaacactatc acgggattaa cagtggcatc
    32341 gcatccgcag agatgctttc tcgtggcagt gaaaatttca acatataaga aaaagtcacc
    32401 tgcaaaatgg aaaacaatcg caatttccct gccagacaat ttcattcgct cacgttcttt
    32461 gccggtcttt gtattggtat tacgcctgtg gcacaggcac tcgccgccga agggcaagct
    32521 aacgcggatg acacgctggt tgtcgaagca tcaacgcctt cgctttatgc gccacaacaa
    32581 tctgccgatc cgaaattctc gcgtccggta gcggatacta cccgcacgat gacggtgatt
    32641 tctgaacaag tgattaaaga tcagggcgca accaacctta ccgacgcg
//
LOCUS       DAJTYF010000033        32459 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTYF010000033 DAJTYF010000000
VERSION     DAJTYF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.033"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            70..1347
                     /gene="waaA"
                     /locus_tag="OO590_001677"
     CDS             70..1347
                     /gene="waaA"
                     /locus_tag="OO590_001677"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6637080.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1355..1834
                     /gene="coaD"
                     /locus_tag="OO590_001678"
     CDS             1355..1834
                     /gene="coaD"
                     /locus_tag="OO590_001678"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6637081.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="OO590_001679"
     CDS             complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="OO590_001679"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6637082.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="OO590_001680"
     CDS             complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="OO590_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6637083.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="OO590_001681"
     CDS             complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="OO590_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6637084.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3421..4089)
                     /gene="radC"
                     /locus_tag="OO590_001682"
                     /pseudo
     CDS             complement(3421..4089)
                     /gene="radC"
                     /locus_tag="OO590_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            4261..5481
                     /gene="coaBC"
                     /locus_tag="OO590_001683"
     CDS             4261..5481
                     /gene="coaBC"
                     /locus_tag="OO590_001683"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6637085.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5459..5917
                     /gene="dut"
                     /locus_tag="OO590_001684"
     CDS             5459..5917
                     /gene="dut"
                     /locus_tag="OO590_001684"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6637086.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6024..6620
                     /gene="slmA"
                     /locus_tag="OO590_001685"
     CDS             6024..6620
                     /gene="slmA"
                     /locus_tag="OO590_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6637087.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="OO590_001686"
     CDS             complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="OO590_001686"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6637088.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7364..8080)
                     /gene="rph"
                     /locus_tag="OO590_001687"
     CDS             complement(7364..8080)
                     /gene="rph"
                     /locus_tag="OO590_001687"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6637089.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8207..9070
                     /locus_tag="OO590_001688"
     CDS             8207..9070
                     /locus_tag="OO590_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6637090.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9291..10115
                     /gene="dinD"
                     /locus_tag="OO590_001689"
     CDS             9291..10115
                     /gene="dinD"
                     /locus_tag="OO590_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6637091.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10406..11023
                     /locus_tag="OO590_001690"
     CDS             10406..11023
                     /locus_tag="OO590_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6637092.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11020..12537)
                     /gene="ligB"
                     /locus_tag="OO590_001691"
     CDS             complement(11020..12537)
                     /gene="ligB"
                     /locus_tag="OO590_001691"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6637093.1"
                     /translation="MEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTG
                     VRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQK
                     VSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTL
                     NSLAVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLP
                     FVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVA
                     SLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLGGQGIPRIDDVVWRGAERTK
                     PTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEH
                     IFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASD
                     ERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(12559..12816)
                     /locus_tag="OO590_001692"
     CDS             complement(12559..12816)
                     /locus_tag="OO590_001692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637094.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFPPATANKTVGR"
     gene            12956..13579
                     /gene="gmk"
                     /locus_tag="OO590_001693"
     CDS             12956..13579
                     /gene="gmk"
                     /locus_tag="OO590_001693"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6637095.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13634..13909
                     /gene="rpoZ"
                     /locus_tag="OO590_001694"
     CDS             13634..13909
                     /gene="rpoZ"
                     /locus_tag="OO590_001694"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6637096.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13928..16036
                     /gene="spoT"
                     /locus_tag="OO590_001695"
     CDS             13928..16036
                     /gene="spoT"
                     /locus_tag="OO590_001695"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6637097.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16043..16732
                     /gene="trmH"
                     /locus_tag="OO590_001696"
     CDS             16043..16732
                     /gene="trmH"
                     /locus_tag="OO590_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6637098.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16738..18819
                     /gene="recG"
                     /locus_tag="OO590_001697"
     CDS             16738..18819
                     /gene="recG"
                     /locus_tag="OO590_001697"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6637099.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18853..20058)
                     /gene="gltS"
                     /locus_tag="OO590_001698"
     CDS             complement(18853..20058)
                     /gene="gltS"
                     /locus_tag="OO590_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6637100.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20338..21729
                     /gene="xanP"
                     /locus_tag="OO590_001699"
     CDS             20338..21729
                     /gene="xanP"
                     /locus_tag="OO590_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6637101.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21850..23559
                     /locus_tag="OO590_001700"
     CDS             21850..23559
                     /locus_tag="OO590_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6637102.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23612..25930)
                     /gene="yicI"
                     /locus_tag="OO590_001701"
     CDS             complement(23612..25930)
                     /gene="yicI"
                     /locus_tag="OO590_001701"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6637103.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25940..27323)
                     /locus_tag="OO590_001702"
                     /pseudo
     CDS             complement(25940..27323)
                     /locus_tag="OO590_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27616..27710
                     /locus_tag="OO590_001703"
     tRNA            27616..27710
                     /locus_tag="OO590_001703"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27650..27652,aa:Sec,seq:tca)
     gene            28010..29194
                     /locus_tag="OO590_001704"
     CDS             28010..29194
                     /locus_tag="OO590_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6637104.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSALIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29606..30649
                     /locus_tag="OO590_001705"
     CDS             29606..30649
                     /locus_tag="OO590_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6637105.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSRIQYQ"
     gene            31164..31592
                     /locus_tag="OO590_001706"
                     /pseudo
     CDS             31164..31592
                     /locus_tag="OO590_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31622..32459)
                     /locus_tag="OO590_001707"
                     /pseudo
     CDS             complement(31622..32459)
                     /locus_tag="OO590_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
BASE COUNT         8120 a         8580 c         8051 g         7708 t
ORIGIN      
        1 gaaaagtaat ggtaaagcca cagctaaata catagaatcc ccagcacatc cataagtcag
       61 ctatttacta tgctcgaatt gctttacacc gcccttctct accttattca gccgctgatc
      121 tggatacggc tctgggtgcg cggacgtaag gctccggcct atcgaaaacg ctggggtgaa
      181 cgttacggtt tttaccgcca tccgctaaaa ccaggcggca ttatgctgca ctccgtctcc
      241 gtcggtgaaa ctctggcggc gatcccgttg gtgcgcgcgc tgcgtcatcg ttatcctgat
      301 ttaccgatta ccgttacaac catgacgcca accggttcgg agcgcgtaca atcggctttc
      361 gggaaggatg ttcagcacgt ttatctgccg tatgacctgc ccgatgcgct caaccgtttc
      421 ctgaataaag tcgaccccaa actggtgttg attatggaaa ccgaactatg gcctaacctg
      481 attgcggcgc tacataaacg taaaattccg ctggtgatcg ctaacgcgcg actctctgcc
      541 cgctcggccg caggttatgc caaactgggt aaattcgtcc gccgcttgct gcgtcgtatt
      601 acgctgattg ctgcgcaaaa tgaagaagat ggtgcacgtt ttgtggcgct gggcgcaaaa
      661 aataaccagg taaccgttac cggtagcctg aaattcgata tttctgtaac tccgcagttg
      721 gctgctaaag ccgtgacgct acgccgccaa tgggcaccac accgcccggt atggattgcc
      781 accagcactc acgaaggtga agagagcgtg gtcattgccg cacatcaggc attgttacag
      841 caattcccga atttattgct catcctggta ccccgtcatc cagaacgctt cccggatgcg
      901 attaaccttg tccgccaggc tggactaagc tatatcacac gctcttcagg ggaagtcccc
      961 tccaccagca cgcaggttgt ggttggcgat acgatgggcg agttgatgtt actgtacggc
     1021 attgccgatc tcgcctttgt tggcggttca ctggttgaac gtggtgggca taatccgctg
     1081 gaagctgccg cacacgctat tccggtattg atggggccgc atacttttaa ctttaaagac
     1141 atttgcgcgc ggctggagca ggcaagcggg cttattaccg ttaccgatgc cactacgctt
     1201 gcaaaagagg tttcctcttt actcaccgac gccgtttacc gtagtttcta tggccgtcat
     1261 gccgttgaag tactgtatca aaaccagggc gcgctacagc gtctgcttca actgctggaa
     1321 ccttacctgc caccgaaaac gcattgaggt tgttatgcaa aaacgggcga tttatccggg
     1381 tactttcgat cccattacca atggtcatat cgatatcgtg acgcgcgcca cgcagatgtt
     1441 cgatcacgtt attctggcga ttgccgccag ccccagtaaa aaaccgatgt ttaccctgga
     1501 agagcgtgtg gcactggcac agcaggcaac cgcgcatctg gggaacgtgg aagtggtcgg
     1561 gtttagtgat ttgatggcga acttcgcccg taatcaacac gctacggtgc tgattcgtgg
     1621 cctgcgtgcg gtggcggatt ttgaatatga aatgcagctg gcgcatatga atcgccactt
     1681 aatgccagaa ctggaaagtg tgtttctgat gccgtcgaaa gagtggtcgt ttatctcttc
     1741 atcgttggtg aaagaggtgg cgcgccatca gggcgatgtc acccatttcc tgccggagaa
     1801 tgtccatcag gcgctgatgg caaaattagc gtagcgttta tgccggatgg tatgccatcc
     1861 ggctcgcatg aattacttct ggcactgccg acaataaaac gttgcccgct gcgcatgttt
     1921 agtcgccaca atcggcgtac cgcacacccg acacggctca ccttttcgcc cgtaaacctg
     1981 caattcctgc gcgaaatagc ccggtttacc atcactttgc agaaaatctt tcagcgttgt
     2041 accaccctgc tcaatcgaac gcagcaacac cgctttaatc acccgagcta acaattcaca
     2101 ctctgccagc gacagtgatg acgccagccg atccggatgg atccccgccg caaacagtga
     2161 ttcgctggca tagatattcc ctacccctac caccagcttg ttatccatca gccacggttt
     2221 aatcgccgtt tttttcttcg cgcacttctg atgcagatac tcaccattga aatcgtcgct
     2281 aagcggctcc ggtccaagat gggccagcac attatgccct tccagctctt tggtccacag
     2341 ccaggcacca aagcggcgcg gatcggtgta gcgcagcact ttaccgttgc tcatcaccaa
     2401 atccacatgg tcatgctttt cagggggaag ttcttctgga aggatgcgca ggctgccaga
     2461 catccccaaa tgaatgataa tccagccctc aggcagctcc agcagcagat atttagcccg
     2521 ccgctgcacg ctaagcactg gttgatcgct taatcggtag atctcttctg aaaccggcca
     2581 gcgcaagcgt ccgttgcgca ccaccgcatg aagaatggtt gcaccaacga gattcggttc
     2641 tatgccgcga cggctggttt caacttcggg taattcaggc atagcatctc caggaatgaa
     2701 cagatgcaat caatatgggg gcaagcagat aacaaaaaac cccgccggag cgaggttttt
     2761 cgttacatca aagcgagaat tatttgattt tcgcttcttt gtagatcacg tgctgacgaa
     2821 caactggatc gaattttttc agttccagtt tttccggctt agtacgtttg ttcttcgtag
     2881 tggtatagaa gtgaccagta ccagcagaag aaaccagctt gattttctca cgaatacctt
     2941 tagccatgat ttatttcctc taagtactta gtacttttcg ccacgggcac gcagttcagc
     3001 cagaactgta tcgatgcctt ttttatcgat tacacgcata cctttagcag atacacgcag
     3061 ggtgacaaaa cgcttctcgc tctcaaccca gaaacggtga gagtgcaggt tcggcaggaa
     3121 acggcgttta gtcgcgttca gtgcgtggga acggttgtta ccggtcaccg gacgcttgcc
     3181 agtaacttgg cagactcggg acatgtctat tctccaaaaa tcaaattagc tcgagcttcg
     3241 tatggggtat tggcgcctcg tcaggcttta cagcccggtc atcgcagttc tatgtgaact
     3301 ctcgattgcc aggcccaaat gccaaacccg agattctcaa aggtggcgta gtatacgctg
     3361 actcagcgat gtgctcaagt cccgaacaga caaagatccc gaaggatcgc gcatagcggg
     3421 ttaaatccag ccgcgttcgg caaaagaaac atactctcca cgcccaatca cgatatggtc
     3481 gagcacgcgt aaatccatga actgacaact ctttattatc cgttcagtaa tgagtttatc
     3541 cgctttactg ggttcagcac aacccgaagg gtgattatgt gcaaggatca gcgccgaggc
     3601 gtttattttt atcgcttagc ggataatttc ccgaggatgg acttcaacat ggtttagcgt
     3661 gccggaaaaa agacggctat gcgttataac ccggtgttgt gagtcgagaa agatcaccat
     3721 aaagatctcc cgctcctcac ccgtgagctg gctttgtaaa aattcccgcg tcatctccgg
     3781 gctgagtaaa gggctttctt cacgcatccg cacgttgtag taacgtcgcg ccagttcagc
     3841 aatccccttt aactgggcaa atttcgctac gccaattcca tgaacaccac taaattgttc
     3901 atattcagag gttaacaagc cataaagaga gccgaaattc tccagcatct cttttgccag
     3961 ggttaataca tctttaccgc gcgttccggt acgcagaaat agcgccagca gctcgacatc
     4021 cgttaaggcg ctaataccaa acttcagcat tttttcgcgc ggcatcaaca gctgtgcatt
     4081 gtttttcacc ttcacctcct ttgtggtgct cgcatcctgt catagccgtt ccgttaaatc
     4141 gacggccagt tttcaatcct ggaaagagcc tcgcaaagtg aatctcaggt gaaggcacga
     4201 caacaaaagt attgtgataa aatcgccaac ttctggtgtc acccaacagg aaaaatcatc
     4261 atgagcctgg ccggtaaaaa aatcgttctc ggcgttagcg gcggtattgc tgcctataaa
     4321 acccctgaac tggtgcgtcg tttgcgcgat cgcggggccg acgtccgcgt agccatgacc
     4381 gaagcggcaa aagcctttat caccccactt agcttgcagg cggtttctgg ttatcccgtt
     4441 tccgacagtc tgcttgaccc ggcagccgaa gcagctatgg gccatattga gctgggtaaa
     4501 tgggctgatt tagtgattct cgcccctgcc acggcagatt tgattgcccg tgttgctgcc
     4561 ggaatggcga atgacctggt atcgacgatt tgtctggcta cacctgcgcc tgtagccgtg
     4621 ctccccgcca tgaaccagca gatgtaccgt gccgctgcca cgcagcataa tttagaggtg
     4681 cttgcttccc gtggtttgct catctggggg ccagacagtg gcagtcaggc ttgtggtgat
     4741 atcggtcctg gacgaatgct cgatccgtta accattgtgg atatggcggt ggcgcatttt
     4801 tcgcccgtca acgacctgaa acatctgaac attatgatta ccgccggtcc gacgcgtgaa
     4861 ccgctcgatc cggtgcgtta tatctctaat cacagctccg gcaagatggg ttttgctatc
     4921 gccgccgctg ctgcccgtcg tggcgcgaac gtcacgctgg tatcaggtcc agtttcacta
     4981 ccgacgccac cgtttgttaa acgtgttgat gtgatgaccg cgctggaaat ggaagccgcc
     5041 gtgaatgctt ctgtacagca gcaaaatatt tttatcggtt gcgccgccgt ggcggattat
     5101 cgcgcagcta ccgtggcccc agaaaaaatc aaaaagcagg ccacgcaggg tgatgaatta
     5161 acaataaaaa tggttaaaaa ccccgatatc gtcgcaggcg ttgccgcact aaaagaccat
     5221 cgaccctacg tcgttggatt tgccgccgaa acaaataatg tggaagaata cgcccggcaa
     5281 aaacgtatcc gtaaaaacct tgatctgatc tgcgcgaacg atgtttccca gccaactcaa
     5341 ggatttaaca gcgacaacaa cgcattacac cttttctggc aggacggaga taaagtctta
     5401 ccgcttgagc gcaaagagct ccttggccaa ttattactcg acgagatcgt gacccgttat
     5461 gatgaaaaaa atcgacgtta agattctgga cccgcgcgtt gggaaggaat ttccgctccc
     5521 gacttatgcc acctctggct ctgccggact tgacctgcgt gcctgtctcg acgacgccgt
     5581 agaactggct ccgggtgaca ctacgctggt tccgaccggg ctggcgattc atattgccga
     5641 tccttcactg gcggcaatga tgctgccgcg ctccggattg ggacataagc acggtatcgt
     5701 gcttggtaac ctggtaggat tgatcgattc cgactatcag ggccagttga tgatttccgt
     5761 gtggaaccgt ggtcaggaca gcttcaccat tcaacctggc gaacgcatcg cccagatgat
     5821 ttttgttccg gtagtacagg ctgaatttaa tctggtggaa gatttcgacg ccaccgaccg
     5881 cggtgaaggc ggctttggtc actctggtcg tcagtaacac atacgcatcc gaataacgtc
     5941 ataacatagc cgcaaacatt tcgtttgcgg tcatagcgtg ggtgccgcct ggcaagtgct
     6001 tattttcagg ggtattttgt aacatggcag aaaaacaaac tgcgaaaagg aaccgtcgcg
     6061 aggaaatact tcagtctctg gcgctgatgc tggaatccag cgatggaagc caacgtatca
     6121 cgacggcaaa actggccgcc tctgtcggcg tttccgaagc ggcactgtat cgccacttcc
     6181 ccagtaagac ccgcatgttc gatagcctga ttgagtttat cgaagatagc ctgattactc
     6241 gcatcaacct gattctgaaa gatgagaaag acaccacagc gcgcctgcgt ctgattgtgt
     6301 tgctgcttct cggttttggt gagcgtaatc ctggcctgac ccgcatcctc actggtcatg
     6361 cgctaatgtt tgaacaggat cgcctgcaag ggcgcatcaa ccagctgttc gagcgtattg
     6421 aagcgcagct gcgccaggta ttgcgtgaaa agagaatgcg tgagggtgaa ggttacacca
     6481 ccgatgaaac cctgctggca agccagatcc tggccttctg tgaaggtatg ctgtcacgtt
     6541 ttgtccgcag cgaatttaaa taccgcccga cggatgattt tgacgcccgc tggccgctaa
     6601 ttgccgcgca gttgcagtaa tatgacgccg gatgactttt catccggcga gtttctttaa
     6661 acgccaaact cttcgcgata ggccttaacc gccgccagat gttccgccat ttccggcttc
     6721 tcttccagat aggcaatcag atctttcagg gtgatgatag agatcacttt gcagttgtaa
     6781 tcacgctcaa cttcctgaat cgccgaaatc tcgccgcgcc cgcgttcctg acgatcgagc
     6841 gaaatcaaca cgccagcaag cgtcgcgcca ttggcctgaa taatctccat cgactcgcga
     6901 atcgccgttc cggcggtgat cacatcatct accagcatta cgcgtccttg taacgcgcta
     6961 ccaaccagat tgccgccttc accgtggtct tttgcttctt tgcggttaaa gcagtacggc
     7021 aggtccaggt cgtgatgctc cgccagtgcc acagcggttg tggtggcaat cgggatccct
     7081 ttgtaagcag ggccaaacag cagatcgaac tcaatgccgg aatccaccaa cgcttcagcg
     7141 taaaaacggc ctaacagtgc cagatcgcgc ccggtattaa acagcccggc gttgaagaaa
     7201 taggggcttt tgcgcccgga tttcagcgta aactcgccaa actttaacac ctgcttgcta
     7261 agcgcaaatt caataaactg gcgctgatat ggtttcatgc cttcgctcct catcttactt
     7321 ttctacagac aaaaaaaagg cgactcatca gtcgccttaa aaatcagttt gccagcgccg
     7381 ccttctgcgt cgctacaatg gattcgattc cccctcgggc cagcgccaac aaggtgagta
     7441 gctcttcatg ggtgaacggc tcgccttctg ccgtcccctg cacttcaatg atgcgcccgt
     7501 cttcggtcat cactacgttc atgtcggtct ctgcggctga gtcttcaacg tattccagat
     7561 cgcaaattgc ttcaccgttt acaataccga cagaaactgc ggctaccatc cctttcatcg
     7621 ggttggtttt cagcttgccg ttttccacca gcttctgtag cgcatctgcc agcgccacgc
     7681 aggcacccgt aatcgacgcg gtacgagtgc caccatcagc ctgaagcacg tcgcagtcca
     7741 gcgtaatggt gaactcaccc agcgctttca aatctactgc cgcgcgaaga gcacgggcga
     7801 tcagacgctg gatttccatt gtgcgtccac cctgcttacc tttcgccgct tcacgagcgt
     7861 tacgggtgtg ggtagaacgt ggcagcatgc cgtactctgc ggtgatccag ccctggccct
     7921 gacctttcag gaagcgcggc acgccttctt caatagaggc ggtacacaat actttggtat
     7981 cgccaaattc gaccagcacc gagccttctg catgttttgt atagttacga gtcagggtaa
     8041 cgggacgcac ctgattattg ctacggcctg ctggacgcat attgaaatct ccggcttgaa
     8101 acaaatgtgg ctgcgcatta tacggacttc cggcggttat tcctatcctg acaaggcatc
     8161 gatggctata atccttccac ctctcctttt ataaacagga acgtctatga tccgcagtat
     8221 gaccgcctac gcccggcgtg aaatcaaggg tgaatggggg agcgcaacct gggaaatgcg
     8281 ctcggtaaac cagcgttatc tggaaactta ctttcgtctg ccggagcagt tccgtagcct
     8341 tgaacctgtc gttcgcgagc gtattcgttc tcgcctgacg cgcggtaaag tggaatgtac
     8401 cctgcgctat gagccagatg ttagcgcaca aggtgagctg atcctcaatg aaaaactggc
     8461 taaacagctg gtaactgccg cgaactgggt aaaaatgcag agtgacgaag gggaaatcaa
     8521 cccggttgat attctacgct ggccgggcgt gatggcagcc caggagcagg atctcgacgc
     8581 gattgccgct gaaattctcg cggcgttgga tggtacgctg gacgacttta tcgtcgcgcg
     8641 cgaaaccgaa ggtcaggcac tgaaagcatt gatcgagcag cgtctggaag gcgtcaccgc
     8701 cgaagtggtc aaagtccgcg cccatatgcc ggaaattctg caatggcagc gtgagcgtct
     8761 ggtcgcgaag ctggaagatg ctcaggttca gttggaaaac aaccgtctgg agcaggagtt
     8821 ggttctgctg gcacaacgaa ttgacgttgc cgaagagctg gaccgcctcg aagcgcatgt
     8881 taaagagacg tacaacattc tgaagaaaaa agaagcggtt ggtcgtcgtc tggactttat
     8941 gatgcaggag ttcaaccgtg agtcgaacac tcttgcgtcg aaatctatca atgccgaagt
     9001 gacaaactcc gccatcgagc tgaaagtgtt gattgagcag atgcgcgagc agattcagaa
     9061 tattgagtaa ctcgtaacac caattctgac ttttcagcct tcacaaatgc cgcccactca
     9121 aacagagcgg catttttctt ccccgcaact tcaattctgt tttgcgtgcc tgctccagat
     9181 tttgcgatgt ttttttgccc agcacactga gaacgtgaga tactcacaac tgtatataaa
     9241 tacagttaca gatttacttt ctttgcaatc gatatcacat ggagtgggca atgaacgaac
     9301 atcatcaacc ttttgaagag ataaaactga ttaatgcaaa cggagcagaa caatggtcag
     9361 caagacaact tgggaaacta ctgggttatt cagagtatcg tcactttata cctgtattaa
     9421 cgcgcgccaa agaggcctgt gagaacagtg gtcacacaat tgatgaccat ttcgaggaga
     9481 tcctcgatat ggtcaaaatt ggctcaaatg ccaaaagagc attaaaagac atcgtactct
     9541 cccgctatgc ctgttacctg gtagtacaaa acggcgaccc cgcgaaaccg gtcattgcgg
     9601 cagggcagac ttattttact atccagaccc gacggcagga gcttgctgac gatgaagcat
     9661 ttaagcaact tcgtgaagac gaaaagcgtc tgtttctgag aaatgagttg aaagaacata
     9721 acaaacaatt ggttgaggca gcacagcaag ctggggtagc gacagctact gatttcgcca
     9781 tatttcagaa tcatggttac caggggctgt atggtggatt agatcagaaa gccatccacc
     9841 aactgaaggg gctgaaaaag agtcagaaga tcctggatca tatgggttca acagaactgg
     9901 cggctaatct ctttcgagct acccaaacag aagaaaaact caagcgggat agcgtgaatt
     9961 caaaacagca agctaatacc acacactttg acgtgggtag caaggtgagg caaaccattc
    10021 aggaacttgg cggaaccatg cctgaggagt tgccgacccc gcaggtcagc atcaagcagt
    10081 tggaaaacag tgtaaaaatt acagagaaga aatagcagaa acgaaaaact ctttttgttg
    10141 attgagacac ccgatgcgta agccaaggtc caggtgcagt gaacacaatg gctaaacatt
    10201 gcacctttct tttcccccct cagttttaac ctattttttc ttatgcattt tctcagacaa
    10261 gaagtcagaa gaatgcatct acgctacaga aaatagcgat ttcacataac taaaagttat
    10321 caatttcctc tcaccttaaa aaatatcaat cgtgacaatg cgcacaaatc gctaccctgc
    10381 cagacagatt tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcattac
    10441 tgccgaagcc atgacggggg cgctggcggc cgggcgacgg cgcatggata catttggcgt
    10501 aattattatt gcaaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg
    10561 ccactatccg ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc
    10621 agtactcacc actatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct
    10681 cgatgcgctc ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg
    10741 gcacggccca attattgccg ttgtcgcggc ggtaaccacc ggtgtatttg gcggcgtttt
    10801 gcgcgatatg ttctgtaaac gcattccgct ggtctttcaa aaagagttgt atgccggtgt
    10861 ctcatttgcc tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt
    10921 ggtgattatc tccaccctgg tattcggctt ttttgctcgt ttactggcgc tacgtcttaa
    10981 gctgggatta ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat
    11041 ctgctgggca gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat
    11101 aacctgtctg gcgcgcccag atccagtccc cggcagctgc tgccagaact gctccgtgct
    11161 aaataaaagt tgcgaccagg atcgttcatc actggcatta agcgccgccc gggttagcgg
    11221 tattcccatt gccatcaccc agcgagtaaa aggctggtta cgagccagat taaactgatg
    11281 ccataactgc gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt
    11341 taataaaagc caggaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc
    11401 ggcctcacca atgccatcca gcccgagaac ctgttttgac cccagccaga ctaagcgtga
    11461 aatgaactgt tcctgacaaa catcagaagc aaagtagcag gtcaacgagt taaagcggtt
    11521 ttctggtggt gtcggttttg tacgttctgc accgcgccag accacatcat caatgcgagg
    11581 aataccctga ccgccaaggc tgacgaggat ctgatcacca ggcgcaatat cccactcctg
    11641 ccagcgcctg acggaaccaa tattcacccg ctggactttt ttatcatcca gcatgacagg
    11701 tgcgagtgac gcaaccaccg atattttacc gctcttaccc accgcaaact gaatcgcctt
    11761 cacttcggca acctgagcta caggttgata tttccaggcc accagccact ccgcctggcc
    11821 cggtagccaa tgacgggatt cgggttcctt agcccctcgc acaaccacgc catcggtgac
    11881 gaagggtaat ttcgctttcc accactcatt gcgtacgcgc gcaacctcat cagcattttt
    11941 aaccgcacgg gtatacctct gcgttagagt aaaacctgcg gtagccagct cctttaaacg
    12001 atcggtcatt aactgcggtc catccggcca tgcccagaca aaaacagcca gtgagtttag
    12061 cgtgtcgcta ttcccctggc gcatcatcag gccagcaact tttgcgcggg catttattcc
    12121 ccccatttgt tgttggatat gcccctcgcg ctggagaaat atttccccct gaagcgtact
    12181 attggctaaa ggcccgctaa cggtttgcgg cacagcggaa attaagctaa ctttctgcgt
    12241 ccagtcctcg cctttcaggc cattaccgcg actgattgct ttgttcagtt tcccgtcccg
    12301 ataaaccagg gttaccgcaa cgccatcaac ttttggctgc acccaaagat cactacgttc
    12361 tcgcatccac agacttaatg cgtttttatc cgccattttg cgtacacccg tatgggcaac
    12421 cggatgcata accgcaccat ttaaaggcgg catcatcacg tcgcggggct cgctcacaaa
    12481 gcagcgttgc cactgcgtaa gacgggcgct taactgatcg taaacaccgt cttccacctc
    12541 actttttcct tccagtagtc atcgtcccac tgttttattt gctgttgcag gcgggaaatt
    12601 tcttcctgtg ctctggctgg cgaccaggcc ggacagaccg cccacacaga tgattgccag
    12661 cacaagatac ttattaatat cgccatccat actttcatct tcacctccgc tgtagtcagg
    12721 ctggcagata tacaacgtgg tgaaggcaaa gccgagtggc aaaaacggag tctgcgagga
    12781 cgcttcctga gaatcgtctt tattgcagta aatcacaggc aaatgcggaa acagctacgc
    12841 aaaacgcaac aactttgcgc aaaaagtgtg agcaagggct acgtcacatg gccgcgccgt
    12901 gtataataag ctcgtatgta ggctttattt cgctaatcac atacgaaaga tactcatggc
    12961 tcaaggcacg ctttatattg tttctgcccc cagtggcgcg ggtaaatcca gcctgattca
    13021 ggctttatta aaaacccaac cgttgtatga cacccaggtt tctgtttcac acaccacacg
    13081 ccaaccgcgt cctggtgaag tccacggtga acattatttc tttgttaatc atgatgaatt
    13141 taaagaaatg attagcagag atgcgttcct cgaacacgca gaagtttttg gtaattacta
    13201 tggcacttcg cgtgaggcca ttgagcaagt actggcgacc ggtgtcgatg tttttctcga
    13261 tatcgactgg cagggcgcgc agcaaattcg ccagaagatg ccgcacgcgc ggagtatctt
    13321 tattttaccg ccgtccaaaa ttgaactgga ccgccgtcta cgcggtcgcg gtcaggacag
    13381 cgaagaggtc attgcaaagc gtatggcgca agctgttgca gaaatgagcc attacgccga
    13441 atatgattat ctgattgtga atgatgactt cgataccgcg ttgaccgatt tgaagaccat
    13501 tattcgcgcc gaacgtctgc gcatgagccg ccaaaagcag cgtcatgacg ctttaatcag
    13561 caaattgttg gcagactgaa cctgatttca gtatcatgcc cagtcatttc ttcacctgtg
    13621 gagcttttta agtatggcac gcgtaactgt tcaggacgct gtagagaaaa ttggtaaccg
    13681 ttttgacctg gtactggtcg ccgcgcgtcg cgctcgtcag atgcaggtag gcggaaagga
    13741 tccgctggta ccggaagaaa acgataaaac cactgtaatc gcgctgcgcg aaatcgaaga
    13801 aggtctgatc aacaaccaga tcctcgacgt tcgcgaacgc caggaacagc aagagcagga
    13861 agccgctgaa ttacaagccg ttaccgctat tgctgaaggt cgtcgttaat cacaaagcgg
    13921 gtcgcccttg tatctgtttg aaagcctgaa tcaactgatt caaacctacc tgccggaaga
    13981 ccaaatcaag cgtctgcggc aggcgtatct cgttgcacgt gatgctcacg aggggcaaac
    14041 acgttcaagc ggtgaaccct atatcacgca cccggtagcg gttgcctgca ttctggccga
    14101 gatgaaactc gactatgaaa cgctgatggc ggcgctgctg catgacgtga ttgaagatac
    14161 tcccgccacc taccaggata tggaacagct ttttggtaaa agcgtcgccg agctggtaga
    14221 gggggtgtcg aaacttgata aactcaagtt ccgcgataag aaagaggcgc aggccgaaaa
    14281 ctttcgcaag atgattatgg cgatggtgca ggatatccgc gtcatcctca tcaaacttgc
    14341 cgaccgtacc cacaacatgc gcacgctggg ctcacttcgc ccggacaaac gtcgccgcat
    14401 cgcccgtgaa actctcgaaa tttacagccc gctggcgcac cgtttaggta tccaccacat
    14461 taaaaccgaa ctcgaagagc tgggttttga ggcgctgtat cccaaccgtt atcgcgtaat
    14521 caaagaagtg gtgaaagccg cgcgcggcaa ccgtaaagag atgatccaaa aaatcctctc
    14581 ggaaatcgaa gggcgtttgc aggaagcggg aattccctgc cgcgtcagtg gtcgcgagaa
    14641 gcatctttat tcgatttact gcaaaatggt gctcaaagag cagcgttttc actcaatcat
    14701 ggacatctac gctttccgcg tgatcgtcaa tgattccgac acctgttatc gcgtgctcgg
    14761 gcaaatgcat agcctgtaca agccgcgtcc gggtcgcgtg aaagactaca tcgccattcc
    14821 aaaagcgaac ggctatcagt ctttgcacac ctcgatgatc ggcccgcacg gtgtgccggt
    14881 tgaggtccag atccgtaccg aagatatgga tcagatggcg gagatgggtg ttgccgcgca
    14941 ctgggcttat aaagagcacg gcgaaaccag caccaccgcg caaatccgcg cccagcgctg
    15001 gatgcaaagc ctgctggagc tgcaacagag cgccggtagt tcgtttgaat ttatcgagag
    15061 cgttaaatcc gatctcttcc cggatgagat ttacgttttc acaccggaag ggcgaattgt
    15121 cgagctgcct gccggtgcaa cgcccgtcga cttcgcttat gcagtgcata ccgatatcgg
    15181 tcatgcctgc gtgggcgcac gcgttgaccg ccagccttac ccgctgtcgc agccgcttac
    15241 cagcggtcaa accgttgaaa tcattaccgc accgggcgct cgcccgaatg ccgcgtggct
    15301 gaactttgtc gttagctcaa aagcgcgcgc caaaattcgt cagttgctga aaaacctcaa
    15361 gcgtgatgat tctgtaagcc tgggccgtcg tctgctcaac catgctttgg gtggtagccg
    15421 taagctgaat gaaatcccgc aggaaaatat tcagcgcgag ctggatcgca tgaagctggc
    15481 aacgcttgac gatctgctgg cagaaatcgg acttggtaac gcaatgagcg tggtggtcgc
    15541 gaaaaatctg caacatgggg acgcctccat tccaccggca acccaaagcc acggacatct
    15601 gcccattaaa ggtgccgatg gcgtgctgat cacctttgcg aaatgctgcc gccctattcc
    15661 tggcgacccg attatcgccc acgtcagccc cggtaaaggt ctggtgatcc accatgaatc
    15721 ctgccgtaat atccgtggct accagaaaga gccagagaag tttatggctg tggaatggga
    15781 taaagagacg gcgcaggagt tcatcaccga aatcaaggtg gagatgttca atcatcaggg
    15841 cgcgctggca aacctgacgg cggcaattaa caccacgact tcgaatattc aaagtttgaa
    15901 tacggaagag aaagatggtc gcgtctacag cgcctttatt cgtctgaccg ctcgtgaccg
    15961 tgtgcatctg gcgaatatca tgcgcaaaat ccgcgtgatg ccagacgtga ttaaagtcac
    16021 ccgaaaccga aattaatgtt ttatgaaccc aacacgttat gcacgcatct gcgaaatgct
    16081 cgccaggcgg cagcctgatc tgaccgtctg catggagcag gtccacaaac ctcataacgt
    16141 ttctgcgatt attcgtaccg cagatgccgt tggcgtacat gaagttcacg ccgtctggcc
    16201 tggtagccgc atgcgcacca tggcttcggc agcggcgggt agtaacagct gggtacaggt
    16261 gaaaacacac cgcaccattg gcgatgccgt cgctcatctc aaaggccagg gcatgcagat
    16321 tctggcaacc catctttctg ataatgctgt cgatttccgc gaaattgatt acactcgccc
    16381 gacctgcatt ttgatgggac aggagaaaac gggcatcacg caggaagcat tggccctggc
    16441 ggatcaggac atcatcattc cgatgatcgg catggtgcag tcgctgaatg tttccgttgc
    16501 ctcagccctc attctttacg aagcccagcg tcagcggcaa aatgcaggca tgtacctgcg
    16561 tgaaaacagc atgttgccgg aagcagagca acaacgcctg ttgtttgaag gcggctatcc
    16621 ggtgctggcg aaagtcgcaa aacgcaaagg cctgccttat ccccacgtca atcagcaagg
    16681 cgagatcgaa gctgatgccg actggtgggc tactatgcag gctgcagggt aagtgccatg
    16741 aaaggtcgcc tgttagatgc tgtcccactc agttccctaa cgggcgttgg cgcagcactt
    16801 agtaacaaac tggcgaaaat caacctgcat accgtgcagg atctactctt acaccttccc
    16861 ctgcgctacg aagatcgcac ccatctctac cccatcggag aactactgcc gggcgtttat
    16921 gccacggtgg aaggcgaagt gctgaactgc aatatctcct tcggcggtcg gcggatgatg
    16981 acctgccaga tcagcgacgg ttccggcatc ctcaccatgc gctttttcaa tttcagcgcg
    17041 gcaatgaaaa atagcctggc gacgggccgc cgtgtactgg cttatggcga agcaaagcgc
    17101 ggtaaatatg gtgcggagat gatccacccg gaataccgcg tgcagggcga tctcagcacg
    17161 ccagaattac aggaaacgct cacgccggtt tatccaacaa cggaaggcgt aaagcaggcc
    17221 acgctgcgta aattaaccga ccaggcgctg gatctgctcg acacctgcgc cattgaagaa
    17281 ctcctgccgc cggaactgtc acaaggaatg atgacgctac cggaagcgtt gcgcactttg
    17341 caccgcccgc caccgacgct acagcttagc gatctggaaa ccgggcagca tccggcgcaa
    17401 cgtcgtctga ttctggaaga actgctggcg cacaacctca gcatgttagc cttacgtgcc
    17461 ggagcacagc gttttcatgc ccagccgctg agcgccaatg acacgctgaa aaataaactc
    17521 ctcgccgcct taccgttcaa gccaacgggc gcacaggcac gcgtagtggc ggagatcgag
    17581 catgatatgg cgctggatgt gccgatgatg cgcctggtac agggcgatgt aggttccggt
    17641 aaaacgctgg tcgccgccct cgccgcgctg cgtgcgattg cccacggcaa acaggtggca
    17701 ctgatggcac caaccgaatt actcgccgag cagcacgcca ataacttccg caactggttt
    17761 gcaccgctcg gtatcgaagt gggctggctc gccggtaagc agaaaggcaa agcgcgactg
    17821 tctcagcagg aagccatcgc cagcggtcag gtgcagatga ttgtcggtac acacgccatc
    17881 ttccaggaac aggtgcagtt taacggcctg gcgctggtga ttatcgacga acagcatcgt
    17941 tttggcgtgc atcagcgtct ggcattgtgg gagaaaggcc agcagcaggg cttccatccg
    18001 catcagttga tcatgaccgc cacgccgatc ccccgcacgc tggcaatgac ggcgtatgcc
    18061 gatctcgata cctcggtgat agatgagctg ccgccaggcc gcacgccagt gactacggtc
    18121 gctattcctg atacccgccg taccgacatc attgaccgcg tgcgccacgc ctgcataact
    18181 gaaggtcgtc aggcgtactg ggtttgtacg ttgattgaag agtcagagtt actggaagcg
    18241 caggcggcgg aagctacctg ggaagagttg aaactggcgc taccagaatt gaatgttggc
    18301 ctggtacacg ggcggatgaa accagccgag aaacaggcgg tgatggcgtc gtttaaacaa
    18361 ggtgagctac atctgctggt tgccactacc gttattgaag tcggtgttga tgtgcctaac
    18421 gccagcctga tgattatcga aaaccctgag cgtctgggtc tggcgcagtt acaccagctg
    18481 cgtgggcgcg taggtcgtgg cgcggtggct tctcactgcg tgctgctcta caaaacgccg
    18541 ctttctaaaa cggcgcaaat tcgcctgcaa gtgctgcgcg acagtaacga cggttttgtg
    18601 attgcgcaaa aagatctgga gattcgcggc cctggcgaat tgttaggcac gcgtcagacg
    18661 ggtaatgctg aatttaaagt ggcggattta ctgcgcgatc aggcgatgat cccggaagtt
    18721 cagcgcctgg cgcgacatat tcacgaacgt tacccacaac aggcaaaagc cttgatagaa
    18781 cgctggatgc cggagacaga acgttactcg aatgcgtaaa aggcggcggt gagaagaccg
    18841 ccgcttcatc ggttaaccgg caaaaatcgg caacattaaa tacagcttaa tcaccagcgc
    18901 attgacgata tcgataaaga acgcaccgac catcggcacc accaaaaacg ccatgtgcga
    18961 cgggccaaag cgttcagtga tcgcctgcat gttggcgatt gccgttggcg ttgcaccgag
    19021 gccaaaacca cagtgacccg cagccagcac tgccgcatcg tagtttttgc ccatcatgcg
    19081 ccaggtaacg aagatggcat acaacgccat gaagatggtc tgtaccacca gaatcgccag
    19141 catcggcagt gccagcgaag ccagctccca cagtttcagc cccatcaacg ccatcgccag
    19201 gaacaacgac aagcttacgt tacccagcac ggataccgca cgctcaaaga cgcggtaaaa
    19261 gcccattatt gacagaccgt tgctcagaat cacgccaaca aacagtacac agacgaaggt
    19321 cggcagttca aaagcagtgc cagccaaaag ttgcgcaaca attttcccca ccgtcaggca
    19381 gatagcaatc agcgcgatag tttcaatcag caccaacgag gtgatcatgc gtcccacatc
    19441 cggcttttca aacgccgtcg ggacttcctg gtcatccgga ataccgttcg gcgtggtgga
    19501 gtgtttcacc agatagcgcg ccaccggacc gccaatcaag ccgcccagca ccagaccgaa
    19561 cgttgcacag gccatagcca cttccgtcgc attggtgaag ccataacgtt caatgaacaa
    19621 tttactccac gcagcgcccg taccgtgacc gccggaaagc gtaatagaac cggccaacag
    19681 ccccatcagc ggatcaagcc ctaacaggct agccatacca atgccaatgg cattttgcat
    19741 caccaacaga ccaacaacca caatcaagaa gatgccaacc acacgcccac cggcacgcaa
    19801 actggcaatg ttggcgttca ggccaatggt ggcgaagaaa gccagcatta acggatcgcg
    19861 cagggacata tcaaagttga cttcccagcc catgcttttt ttcagtacta gtagcgccag
    19921 cgccaccaac aaaccacccg caacaggttc cggtatggtg tatttcttca aaaaggagac
    19981 ggaatggacc aacttacgcc cgagcagcaa cgtcagcgtt gcggcaacaa gcgttgctaa
    20041 agtatcgaga tgaaacatag ttactccttt gtatccgcat cactctttca tactcgtcat
    20101 acttcatcgc gcagtggcaa cttcatgaac tatgacgggt atcgagttat gctttcctgg
    20161 gtcaaattct cagctgttaa tgaacaaatg gcatggattt taagcagaaa aataccaaaa
    20221 gttatataaa aaagtgcatt tatctcattt aaatatttag atgaggatgt ggcaaacgtt
    20281 tgcttttctc tttcggtcag ctaaaatgcc cgctttgcta ccacgggatt gttttcaatg
    20341 tctgtttcca ccctcgagtc agaaaatgcg caaccggttg cgcagactca aaacagcgaa
    20401 ctgatttacc gtcttgaaga tcgtccgccg cttcctcaaa ccctgtttgc cgcctgtcag
    20461 catctgctgg cgatgttcgt tgcggtgatc acgccagcgc tattaatctg ccaggcgctg
    20521 ggtttaccgg cacaagacac gcaacacatt attagtatgt cgctgtttgc ctccggtgtg
    20581 gcatcgatta ttcaaattaa ggcctggggt ccggttggct ccgggctgtt gtctattcag
    20641 ggcaccagct tcaactttgt tgccccgctg attatgggcg gtaccgcgct gaaaaccggt
    20701 ggtgctgatg ttcctaccat gatggcggct ttgttcggca cgttgatgct ggcaagttgc
    20761 accgagatgg tgatctcccg cgttctgcat ctggcgcgcc gcattattac gccgctggtt
    20821 tctggcgttg tggtgatgat tatcggcctg tcgctaattc aggtggggct gacctccatt
    20881 ggcggcggtt acgcagccat gagcgataac accttcggcg caccgaaaaa tctgctgctg
    20941 gcaggcgtgg ttttagcctt aattatcctg cttaaccgtc aacgtaaccc ttacttacgc
    21001 gtggcctcac tggtaattgc gatggcggcc ggatatgcgc tggcgtggtt tatgggcatg
    21061 ttgccagaaa gcaacgaacc gatgacgcaa gaactgatta tggtgccaac gccgctctat
    21121 tacggtcttg gcattgaatg gagtctgctg ctgccgctga tgctggtctt tatgatcact
    21181 tcgctggaaa ccattggcga tatcacggcg acctctgacg tttccgaaca gccagtgtcc
    21241 ggtccgctgt acatgaaacg cctgaaaggc ggcgtgctgg caaacggcct gaactcgttt
    21301 gtttcggcgg tgtttaacac cttcccgaac tcctgcttcg ggcaaaacaa cggagtgatc
    21361 cagttgactg gtgttgccag ccgctatgtc ggttttgtcg tcgcgctgat gttgatcgtg
    21421 ctgggtctgt tcccggcagt gagcgatttt gtacaacaca ttccagaacc ggttctgggc
    21481 ggcgcaacgc ttgtaatgtt tggcaccatc gccgcctccg gtgtgcgtat cgtttctcgt
    21541 gagccgctga accgtcgggc gattctgatt atcgcgttgt cgctggcggt tggtctgggc
    21601 gtgtctcagc agccgctgat tttgcagttt gcccctgaat ggctgaaaaa cctgctctcc
    21661 tccgggatcg ccgcgggcgg tattactgcc atcgtgctga atctgatttt cccaccagaa
    21721 aaacagtaat tcactggcgg cggtaagata attaccgccg cagtctcacc tctttcacca
    21781 ttcccgcctt gaggattgcg cgtaaatcgt gcataactcc cttatgtgca tttcacggga
    21841 tggaagacca tgaaatttat tgggaagctg cttctctaca ttctcatcgc tctgttagtg
    21901 gtgatcgctg gcctctattt tcttctgcaa acccgctggg gagcagaaca tatcagcgca
    21961 tgggtttccg agaatagcga ctatcatctg gccttcgggg cgatggatca ccgtttttcc
    22021 gcgccatctc atatcgtgct ggagaatgtc acgtttggtc gtgatggtca gcccgcgccc
    22081 ctggtggcaa aaagtgtcga cattgcgcta agcagtcgcc aactgaccga accacgccat
    22141 gtcgatacca tcctgctgga aaacgggacg ctgaatctca ccgaccagac cgcgccgcta
    22201 ccgttcaaag ccgatcgtct gcaactgcgt gatatggcgt ttaatagccc gaatagcgaa
    22261 tggaaactga gcgcgcagcg ggtaaatggc ggcgtggttc cgtggtcacc agaagccggt
    22321 aaagtgctgg gtacgaaggc gcagattcag tttagtgccg gatcgctttc gctcaatgat
    22381 gttcctgcca ccaatgtact gattgaaggc agtattgata acgatcgcgt tacgctgact
    22441 aacctgggtg ccgacatcgc ccgcgggaca ttaaccggaa acgcgcagcg taacgccgac
    22501 ggcagctggc aagtggaaaa tctgcgcatg gcggatatcc gtctacaaag cgaaaaatcg
    22561 ctaaccgact tctttgcgcc attacgctct gtcccgtcgt tgcagattgg tcgcttggaa
    22621 gtcatcgatg ctcgtctgca aggtccggac tgggcggtga ccgacctcga tctcagcttg
    22681 cgcaacatga ccttcagtaa agatgactgg cagacacagg aaggcaaact gtcgatgaac
    22741 gccagcgagt tcatttatgg ttcgctgcat ttatttgacc cgattataaa cgcggaattt
    22801 tccccgcagg gcgtagcgct gcgccagttc accagccgct gggaaggggg tatggtcaga
    22861 acgtcaggga actggctgcg tgacggtaaa acgttgatcc ttgatgatgc ggcaattgcc
    22921 gggctggaat ataccttgcc gaaaaactgg caacagttgt ggatggaaac gacacccggt
    22981 tggttaaaca gcctgcaact gaagagattt agcgccagcc gcaatctgat cattgatatc
    23041 gaccctgact tcccgtggca gctcaccgcg ctcgatggtt acggtgccaa cctgacgctg
    23101 gttaccgatc ataaatgggg cgtctggagt ggctcggcga atctgaatgc cgccgccgcg
    23161 acattcaatc gtgttgatgt tcgtcgcccg tcgctggcgc tgaccgccaa cagcagcacg
    23221 gtgaatatca gcgaactgag tgcatttact gaaaaaggca ttctggaagc caccgcaagt
    23281 gtttcacaaa cgccacaacg tcagacccat atcagcctga atggacgcgg tgtgccggtg
    23341 aatattttgc aacaatgggg atggcctgaa ttacagttga ctggcgacgg caatattcag
    23401 cttaccgcca gtggagatat tcaggccaat gccccgctga aacctacggt tagcgggcaa
    23461 ttgcatgccg tgaacgccgc aaagcagcaa gtgactcaaa ccatgaatgc gggcgtcgtt
    23521 tccagcggtg aagttacatc gacggagccg gtgcggtaaa catcaatctc gccgggtacg
    23581 actcagacgt acccggcatt ccgtcaatag attacaacgt aattgtcagc gcattccctt
    23641 gaggcgtcac caccagcccc tgctcacttt cagcctgcga accgccttgc agaccattta
    23701 ctttcacaac attgcgcaag cacagcgtcc agttcttcgc ctcgcccgca ccagtcacag
    23761 taatcgtgtt gccagtacgt gctgctttta aagtaaagat caccgatccg tcagcagcag
    23821 gcacttcaca gacggcttca tgcccgtctt gcagattaaa gaggtggaat gccgtgcctt
    23881 cgtgccacac gtaatcggga cgttgatcgt tgttgcccag cgccagtaga gtgttatcac
    23941 gcacataaac gggcagactc aggaagccgt gctgctgttt atgccagcga ctaccgtcga
    24001 gttcatcgtt gtgccacagg tgtgtccagc gaccttccgg caggtagaac tgcacatcgc
    24061 ccgcttcagt gaacaccggc gcaaccatca cgttgtcgcc taacatgtat tgacggtcaa
    24121 ggtaatcaca agccggatcg tccgggaact ccatcatcat ggcccgcatc atcggcgtac
    24181 cccgcgcgtt cgcacgcgca gcttcacgat acagatacgg catcatgcgg catttcagtt
    24241 gcgtgaagaa gcgcaccaca tcacaggact catcatcgta ggcccacggc acacgataag
    24301 atttgctacc gtgtaaacgg ctatggctgg agagcaaacc aaacgcgcac cagcgtttgt
    24361 aaacgtgcgc cggagcggta ttttcaaagc cgccgatatc gtggctccag aagccaaaac
    24421 ctgaaaggcc aatagacaaa ccaccgcgca ggctttccgc cattgattcg tagttagcgt
    24481 aacaatcgcc accccagtgt accgggaatt tctgcgcacc gacggaggcc gagcgggcaa
    24541 acaagacagc ttcttcctca ccaacggtgt ccttgagcac gttccacacc agttcgttgt
    24601 agatgtacgc ataatggtta tgcattttct gcggatcgga accgtcaaac cactgaacat
    24661 cagttgggat acgttcgcca aagtcggtct taaagcaatc aacgcccatc gcgaccagac
    24721 ctttcagttt gtcggcgtac catttgcagg catccggatt ggtaaagtca taaatcgcca
    24781 gacctggctg ccatttatcc cactgccata gcgaaccgtc cgggcgtttg agtaaatagc
    24841 ctttctcttg taactcttta aagacggggg atttttgacc gatatagggg ttaatccaga
    24901 cgcagatttt cagccctttc gctttcaggc ggcggatcat cccttccggg tcagggaaag
    24961 tcagcgggtc ccactcaaaa tcgcaccact ggaaggcttt catccagaaa cagtcaaagt
    25021 ggaaaacatg cagcggcaga ttgcgttccg ccataccatc gataaagctg tttaccgtcg
    25081 cttcgtcgta gttggtggta aatgaagtgg ttagccacag gccgaaggac cacgggggcg
    25141 gcagcgccgg acgaccagta aagcgggtat aacgatcaag taccgctttc ggcgtcgggc
    25201 cgtcgataac aaagtattcg agatattcac tctcaacgct gaactgcact ttggagactt
    25261 tctccgatcc cacttcaaaa gagacacact ggggatgatt gaccagtacc ccataaccac
    25321 ggttagtcat gtagaacggg atatttttat acgcctgttc agtacttgtg ccgccgtccc
    25381 ggttccaggt ctctaccgtc tggccattgc gcaccagggc agtaaagcgc tctcccagac
    25441 cgtaaactgt ttcgccaacg ccaagatcaa gccgctcaaa catataattg cgttgattat
    25501 tcgtgtcctg cacgtagcca ttatttttca cctgactacc ggtaatacgt tcgccgttgc
    25561 gcagaaaatc cagtgaccag aactcacctt tgctgacacg cgcgcttaag ttgccacttt
    25621 taaactcagc ataacgttct gtgttttcga ttgtgacctt cacgtcctgc aaaatattga
    25681 gcggataatg aggaccgtta ttcagcgccc cctgaaaata ctcaatccgc acaccgacaa
    25741 taccttcctg tggggagaaa aagcgcagcg taaataaagg cgtatcaagc tgccaggtac
    25801 gttcacgcac atcacggggg gcagcataga ccaccatttc attatcctgc tgttcaacct
    25861 cgaatacctg aagcgggtga atcaaattga gcccaggttg aatcaaccag tttccatcgc
    25921 taattttcat tgccgttcct tagttctgca cttcttgaga ggtgaattgt tgctggcaac
    25981 ggcgtttacc ctgagccagc tgttccataa cggttttcag attgtgcgtc gtgagtgagt
    26041 agtagcgttt agcgataatc gcgctcagca aataacagat cgccggaaca atcgtgaata
    26101 gcgcaataat gatgctaatc gtggcgctgt tctgcgcttt ttctgccgca tcatatccgc
    26161 cataagccag catccagccg ataagagcgc cgccgaaggc caaccccagt ttgagcacaa
    26221 acagcgtgcc agcaaaactg atcccggtca ggcgtttacc attgcaccac tcgccgtagt
    26281 cgacggtatc gaacatcatt acccactgga taggtgtcac cagttgatgc aacacaccaa
    26341 tcacgaagat gaagacaaac atagtgatgc tggcctgcat gggaacaaag aacatcgcga
    26401 ggctaatcac tgccagcagg gcgttcgtcc accagaagat agtgacttta catttccagt
    26461 cggtcagagg ttttgccagt gcggaaccaa tcaggttacc cacgcaataa gtggtgagaa
    26521 aagcgacaaa cacttccggc gtgcccaaaa tccatgtgac gtaatacatc atcgccccac
    26581 cgcgtacgca caccgccagg atattgaaaa tggttagtaa accgacaatc cgccactggt
    26641 cgttttgcca gatatcacgt aaatcttccc gcatagacgt tgttgtaggt ggtgcttcaa
    26701 cgcgttcttt agtggtgaag aaacaaaatg ccagcatcat taatgccacc acggaaagga
    26761 ccacgatacc gccctggaaa ccgagtggtt tattatcacc gccaattaaa ttaaccagtg
    26821 gcatcatcag aacagtagaa agcatgcctc ccgcggtcgc cagcacaaaa cgccaggatt
    26881 gcagcgagat acgctgagtc gggtcattgg tgattacacc acccaatgcg cagtaaggga
    26941 tattgacgac ggtataaagt aaggtaagtt agggtgtaag taattgctgc atagatcatt
    27001 ttgccgttca tactgagatc tggcgtgcta taggccagta cacagacgat cccgaatggc
    27061 agtgcgccaa acagtaccca cggacgaaat ttaccccagc gagagcgcgt tcgatcggcc
    27121 aacaacccca tgcaaggatc ggaaatcgca tccagtgcac gagcgaccaa aaacatggtt
    27181 ccgacaaatc cggcaggaat gccaaaaata tcggtataaa agaacatcat atataacatt
    27241 acgttatcga aaataatgtg gctggcggcg tctcccatgc cataaccaat tttctcttta
    27301 acggacaaca cttcactctt catctgcttt tccttgatgc tataggggtg ctgagaccga
    27361 taacgcattt tgtaaaccat ccctgtgaga gcgaatattc cgatatctgg ttattaattt
    27421 atgattcttg ttttatgtga tcgtggtagc gttaattccg ctcatatatc attgtaaaat
    27481 atgggtttta tataaattat aatgctttcg tgataatacg ctgcgtttat taggcggaaa
    27541 aaactgatct gggggatgta gaaactcaag gaagtagcta taatgcgccc cgcctccatg
    27601 tagcaatcga ggcgcggaag atcgtcgtct ccggtgaggc ggctggactt caaatccagt
    27661 tggggccgcc agcggtcccg ggcaggttcg actcctgtga tcttccgcca gtttacctct
    27721 tctgaactct cccaatgtca ataaatctct tctaatccct tgtatatcag tatttatttg
    27781 tatcctgagg tcaacctact tcaccccgta tcaagtttct tttgggggta ctttaggggg
    27841 tgttatttcc tattgaaata caagcacccc cataatagga aaaatgtgat acataacaac
    27901 ggcttttaac aatatctttg ttatacaagg ggtgtattgg gggtatcagg ttagttactg
    27961 gcagaaatcc ccctcaggaa catgaattca tcaaaagccg gaggatgtca tggcattaac
    28021 cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg ctgatggcgg
    28081 cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga agtaccgctt
    28141 tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc tagccgatgc
    28201 tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc caggggaggc
    28261 aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg aggctattgc
    28321 tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg atgaaatcat
    28381 tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg ccgaaatcaa
    28441 accaatggag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc tggagaaaat
    28501 gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta ctggccgggc
    28561 agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa aagtacattt
    28621 tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg gttataccgg
    28681 aagtataatc actaagacag ctactcagat cattatgtta accggcgtac gaacacaaga
    28741 actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg agatcccggc
    28801 agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg tcattatgct
    28861 atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg gtagaaatga
    28921 tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac ttggatataa
    28981 gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc atgaacaagg
    29041 ctttaattcc gccttgattg aaatgcagtt agctcatgtg gataaaaatt ccatcagggg
    29101 tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat ggtacgctga
    29161 ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc cctgatgttt
    29221 tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg ttattatatt
    29281 atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt gacattttta
    29341 aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca aaggaaaagt
    29401 tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa tgagattcac
    29461 ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag caatattcat
    29521 tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc agactatatc
    29581 accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag tgcacgataa
    29641 caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg agtttgaaga
    29701 agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca acaaactccg
    29761 acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg catatgcatt
    29821 tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac tggcggcagg
    29881 taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc ccgaaaaccg
    29941 gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac agcgccatca
    30001 ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaagata atcagggggc
    30061 tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag ccttcagtgt
    30121 actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc tcctgcattt
    30181 tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg ccacaaacac
    30241 ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg tgtggcatga
    30301 cttctccggc tctctggata cggcagaaga ttactatgca acatactcac cattatgggg
    30361 acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct ccgtgctgca
    30421 tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg atttcgaaat
    30481 atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca agacactttc
    30541 tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc cctggatatt
    30601 gcccttttcc gcagcaggga tgaaacattc aagaatccag tatcagtaga tattgcatta
    30661 gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt tccttcccca
    30721 ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag cgttgtaaat
    30781 tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt tatcgtcaaa
    30841 cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa tcccctcctc
    30901 tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat aaacagtaaa
    30961 aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg tgcttgtgtt
    31021 ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat tgcatccaac
    31081 ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa atcaatggat
    31141 ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc tcatactggc
    31201 gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa gacgcccaaa
    31261 atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt gtcaataaat
    31321 tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc ccaaaagttt
    31381 atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat attgttacac
    31441 cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg ccagcgacct
    31501 ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt ggtgagattg
    31561 ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg tggacacggc
    31621 cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag gcactgtgac
    31681 gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc attatttccc
    31741 ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag aagctttcca
    31801 cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg ttgtgtcgct
    31861 tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta tgaacaatga
    31921 cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag gccagttgtg
    31981 ctgtcattcg cggtgacatc gaccagccaa tgacggcgcg tgaccacagg tcgatgacta
    32041 ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc gcccacttct
    32101 ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac gggcaggcca
    32161 tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag cccctgacga
    32221 cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc aaggcgggga
    32281 gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc gcaggtgagc
    32341 cgaaactgtt ggcgcaggct catctggtga cgacgcctga gccagacata ccagccgctg
    32401 cgggcaaccc gaagtacacg acacatcgct ttgatgctga actctgcccg atgattttc
//
LOCUS       DAJTYF010000034        32077 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTYF010000034 DAJTYF010000000
VERSION     DAJTYF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.034"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..374)
                     /gene="ssb1"
                     /locus_tag="OO590_001708"
     CDS             complement(<1..374)
                     /gene="ssb1"
                     /locus_tag="OO590_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6637106.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGG"
     gene            628..3450
                     /gene="uvrA"
                     /locus_tag="OO590_001709"
     CDS             628..3450
                     /gene="uvrA"
                     /locus_tag="OO590_001709"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6637107.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3485..3841)
                     /locus_tag="OO590_001710"
     CDS             complement(3485..3841)
                     /locus_tag="OO590_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6637108.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(3845..4261)
                     /locus_tag="OO590_001711"
     CDS             complement(3845..4261)
                     /locus_tag="OO590_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6637109.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4372..5085)
                     /gene="aphA"
                     /locus_tag="OO590_001712"
     CDS             complement(4372..5085)
                     /gene="aphA"
                     /locus_tag="OO590_001712"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6637110.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5487..5709
                     /locus_tag="OO590_001713"
                     /pseudo
     CDS             5487..5709
                     /locus_tag="OO590_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6213..7406)
                     /gene="tyrB"
                     /locus_tag="OO590_001714"
     CDS             complement(6213..7406)
                     /gene="tyrB"
                     /locus_tag="OO590_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6637111.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7659..8738)
                     /gene="alr"
                     /locus_tag="OO590_001715"
     CDS             complement(7659..8738)
                     /gene="alr"
                     /locus_tag="OO590_001715"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6637112.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(8791..10206)
                     /gene="dnaB"
                     /locus_tag="OO590_001716"
     CDS             complement(8791..10206)
                     /gene="dnaB"
                     /locus_tag="OO590_001716"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6637113.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10289..11272
                     /locus_tag="OO590_001717"
     CDS             10289..11272
                     /locus_tag="OO590_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6637114.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQPAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11438..11680)
                     /gene="pspG"
                     /locus_tag="OO590_001718"
     CDS             complement(11438..11680)
                     /gene="pspG"
                     /locus_tag="OO590_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6637115.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(11814..12851)
                     /gene="dusA"
                     /locus_tag="OO590_001719"
     CDS             complement(11814..12851)
                     /gene="dusA"
                     /locus_tag="OO590_001719"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6637116.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13214..14199)
                     /locus_tag="OO590_001720"
                     /pseudo
     CDS             complement(13214..14199)
                     /locus_tag="OO590_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14517..15032
                     /gene="zur"
                     /locus_tag="OO590_001721"
     CDS             14517..15032
                     /gene="zur"
                     /locus_tag="OO590_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6637117.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15074..15283)
                     /locus_tag="OO590_001722"
     CDS             complement(15074..15283)
                     /locus_tag="OO590_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6637118.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15399..16724)
                     /gene="dinF"
                     /locus_tag="OO590_001723"
     CDS             complement(15399..16724)
                     /gene="dinF"
                     /locus_tag="OO590_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6637119.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(16797..17405)
                     /gene="lexA"
                     /locus_tag="OO590_001724"
     CDS             complement(16797..17405)
                     /gene="lexA"
                     /locus_tag="OO590_001724"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6637120.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17515..17883)
                     /gene="dgkA"
                     /locus_tag="OO590_001725"
     CDS             complement(17515..17883)
                     /gene="dgkA"
                     /locus_tag="OO590_001725"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6637121.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18054..20477
                     /gene="plsB"
                     /locus_tag="OO590_001726"
     CDS             18054..20477
                     /gene="plsB"
                     /locus_tag="OO590_001726"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6637122.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20632..21504)
                     /gene="ubiA"
                     /locus_tag="OO590_001727"
     CDS             complement(20632..21504)
                     /gene="ubiA"
                     /locus_tag="OO590_001727"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6637123.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21517..22014)
                     /gene="ubiC"
                     /locus_tag="OO590_001728"
     CDS             complement(21517..22014)
                     /gene="ubiC"
                     /locus_tag="OO590_001728"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6637124.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22237..23817)
                     /locus_tag="OO590_001729"
                     /pseudo
     CDS             complement(22237..23817)
                     /locus_tag="OO590_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24046..24966)
                     /gene="malM"
                     /locus_tag="OO590_001730"
     CDS             complement(24046..24966)
                     /gene="malM"
                     /locus_tag="OO590_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6637125.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLSVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25118..26458)
                     /gene="lamB"
                     /locus_tag="OO590_001731"
     CDS             complement(25118..26458)
                     /gene="lamB"
                     /locus_tag="OO590_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6637126.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26530..27645)
                     /gene="malK"
                     /locus_tag="OO590_001732"
     CDS             complement(26530..27645)
                     /gene="malK"
                     /locus_tag="OO590_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6637127.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28010..29200
                     /gene="malE"
                     /locus_tag="OO590_001733"
     CDS             28010..29200
                     /gene="malE"
                     /locus_tag="OO590_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6637128.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29354..30898
                     /gene="malF"
                     /locus_tag="OO590_001734"
     CDS             29354..30898
                     /gene="malF"
                     /locus_tag="OO590_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6637129.1"
                     /translation="MDVIKKKHWWQSDALKWSVPGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITTILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            30913..31803
                     /gene="malG"
                     /locus_tag="OO590_001735"
     CDS             30913..31803
                     /gene="malG"
                     /locus_tag="OO590_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6637130.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8171 a         8217 c         7743 g         7946 t
ORIGIN      
        1 ccacctgccg gagcgccacc accctgacga ccacccagca tctgcatggt gccgccaacg
       61 ttcaccacga cttctgtggt gtagcgatcc tgaccggatt gatcggtcca tttacgggta
      121 cgcagctgac cttcgatata aacctgagaa cctttacgca gatattcgct cgccacttct
      181 gccagtttgc cgaacagcac aacgcggtgc cattcagtct gttctttcat ctcgccggtc
      241 gctttatcac gccaggattc ggaagtagcc agcgtaatgt tggcaactgc gccaccattt
      301 ggcatgtagc gtacttccgg gtcctgaccc agattaccaa cgagaataac cttgtttacg
      361 cctctgctgg ccatgttcgt gtctcctgaa aaaaatcgtt ctgaataagt gtaaacgcgc
      421 gattgtacca ttaccaatag cgcttttact atgttgtgac ctcggttccg ggaaacaaac
      481 ctggccagac attgttacac aacactccgg ataatgcatt ccaatactgt atattcattc
      541 aggtcaattt gtgtcataat taaccgtttg tgatcgccgg tagcaccatg ccaccgggca
      601 aaaaagcgtt taatccggga aaggtgaatg gataagatcg aagttcgggg cgcccgcacc
      661 cataatctca aaaacatcaa cctcgttatc ccccgcgaca agctcattgt cgtgaccggg
      721 ctttcgggtt ctggcaaatc ctcgctcgct ttcgacacct tatatgccga agggcagcgc
      781 cgttacgttg aatccctttc cgcctacgcg cggcagtttc tgtcactgat ggaaaagccg
      841 gacgtcgatc atattgaggg gctttctcct gccatctcaa ttgagcagaa atcgacgtct
      901 cataacccgc gttctacggt ggggacaatc accgaaatcc acgactattt gcgtttgttg
      961 ttcgcccgcg tcggcgaacc gcgctgtccg gaccacgacg tcccgctggc ggcgcaaacc
     1021 gtcagccaga tggtggataa cgtgctgtcg cagccggaag gcaagcgtct gatgctgctc
     1081 gcgccaatca ttaaagagcg caaaggcgaa cacaccaaaa cgctggagaa cctggcaagc
     1141 cagggttaca tccgtgctcg tattgatggc gaagtctgcg atctttccga tccgccgaaa
     1201 ctggaactgc aaaagaaaca taccattgaa gtggtggttg atcgcttcaa ggtgcgtgac
     1261 gatcttaccc aacgtcttgc ggagtcgttt gaaaccgcgc tggaactttc cggtggtaca
     1321 gcggtagtgg cggatatgga cgacccgaaa gcggaagagc tgctgttctc cgccaacttc
     1381 gcctgcccaa tttgcggcta cagtatgcgt gaactggagc cgcgactgtt ttcgtttaac
     1441 aacccggcag gtgcctgccc gacctgtgac ggccttggcg tacagcaata tttcgatcct
     1501 gaccgcgtga tccaaaaccc cgagctgtca ctggctggcg gtgcgatccg tggctgggat
     1561 cgccgcaact tctattactt ccagatgctg aaatcgctgg cagatcacta taagttcgac
     1621 gtcgaagcgc cgtggggcag cctgagcgcg aacgtgcata aagtggtgtt gtacggttct
     1681 ggcaaagaaa acattgaatt caaatacatg aacgatcgtg gcgatacctc catccgtcgt
     1741 catccgttcg aaggcgtgct gcacaatatg gagcgccgtt ataaagagac agaatccagt
     1801 gcggtacgtg aagaattagc caagtttatc agcaatcgcc catgcgccag ctgcgaaggg
     1861 acgcgtctgc gtcgggaagc gcgccacgtt tatgtcgaga atacgccgct gcctgctatc
     1921 tccgacatga gcatcggtca tgcgatggaa ttcttcaaca atctcaaact cgctggtcag
     1981 cgggcgaaga ttgcggaaaa aattcttaaa gagatcggcg atcgcctgaa attcctcgta
     2041 aacgtcggcc tgaattacct gacactttcc cgctcggcag aaacactttc cggcggtgaa
     2101 gcccagcgta tccgtctggc gagccagatt ggtgcgggcc tggttggcgt tatgtacgtg
     2161 ctggacgagc cgtctatcgg cctgcaccag cgcgataacg agcgcctgtt gggtacgctt
     2221 atccatctgc gcgatctcgg taataccgtg attgtggtgg agcatgacga agacgcaatt
     2281 cgcgccgctg accatgtgat cgatatcggt cctggtgcgg gtgttcacgg cggtgaagtg
     2341 gtcgcagaag gtccgctgga agcgattatg gcggtgcctg aatcgttgac cgggcagtac
     2401 atgagcggta aacgcaagat tgaagtgccg aagaaacgcg ttccggcgaa tccagaaaaa
     2461 gtgctgaagc tgacaggcgc acgcggcaac aacctgaagg acgtgacgct cacgctgcca
     2521 gtcggtctgt ttacctgcat cacaggggtt tcaggttccg gtaaatcgac gctgattaac
     2581 gacacactgt tcccgattgc ccaacgccag ttgaatggtg cgaccatcgc cgaaccggca
     2641 ccgtatcgcg atattcaggg actggagcat ttcgataaag tgatcgatat cgaccaaagc
     2701 ccaattggtc gtactccacg ttctaacccg gcgacctata ccggcgtgtt tacgcctgtg
     2761 cgcgaactgt ttgcgggcgt accggaatcc cgtgcgcgtg gttatacgcc aggacgtttc
     2821 agctttaacg tccgtggcgg gcgctgcgaa gcctgtcagg gcgacggtgt gatcaaagtg
     2881 gagatgcact tcctgccgga tatctacgtg ccgtgcgatc agtgcaaagg taaacgctat
     2941 aaccgtgaaa cgctggaaat taagtacaaa ggcaaaacca tccacgaagt gctggatatg
     3001 accatcgaag aggcgcgtga gttctttgat gcggtgccag ctctggcgcg taagctgcaa
     3061 acgttgatgg acgttggtct gacgtacatt cgcctggggc agtccgcaac cacactttct
     3121 ggtggtgaag cccagcgcgt gaagctggcg cgtgagctgt caaaacgcgg caccgggcag
     3181 acgctgtata ttctcgacga gccgaccacc ggtctgcact tcgccgatat tcagcaactc
     3241 ctcgacgtgc tgcataaact gcgcgatcag ggcaatacca ttgtggtgat tgagcacaat
     3301 ctcgacgtga tcaaaaccgc tgactggatt gtcgacctgg gaccagaagg cggcagtggc
     3361 ggcggcgaaa tcctcgtctc cggtacgcca gaaaccgtcg cggagtgcga agcttcgcat
     3421 acggcacgct tcctcaagcc gatgctgtaa tcgttaaggc cgctttctga gcggcctttt
     3481 cctttcagag ttgtaccagc aatttacgct tttcttccgg tagtaaattc actgcctgct
     3541 gataagacgc atccaccaga taatagattt gcgaatccgg cagcgaaccg tcgagataca
     3601 cggtgctcca gtgcgcttta ttcagatggc ggctaggacg cacatcgctg tgctgctgac
     3661 gtagcagctc cgccagctcc gggctggttt tcagcgaaac agctgggcga ttttctactt
     3721 ctttcaccat cgcaaacagt acatcttcca ctttgatctg cgtcgctttc cagtcgttat
     3781 gcacgctctg ttctgcgcct ggttttgcca tgcaatattg tagcaactcc gaaatggtca
     3841 ttttttactc cccttgtagt gtcgcgatga tgcgacgcga tccgccgtgg atgcgatgtt
     3901 cccccagcca gatgccttgc caagtgccgg tctgaatacg ccctttatgt accggcaata
     3961 caagcgatgt tcccagcatt gaggatttga tatgagaagg catatcgtct gctccctcat
     4021 agtcatgctc ataatttccg ttgtcgggaa cggtgcggag gaaaaaacac tccatgtcgt
     4081 ggcgtacggt gggatcgcag ttctcattaa gtgtcagaga ggcggaggta tgttgcagca
     4141 acagatgcag taagccgatg ttaacgcgcg gcatatcagc cagctgattc agaatttcat
     4201 ccgttaccag atgaaaccca cgagatttgg cgctaagcgt gagcgtcttt tgataccaca
     4261 tgtgctgctc cttgataaaa ctctcttaat cagtttgcag caagacagcg aaaggataaa
     4321 ggtgtgatta aaaaaacagc attcgggaga gcatcactct ctcccgctct gtcagtattc
     4381 tgaattgacg atcacctctt caccaaacgc acccgcttgt ggcaagggtt tgtaggtaga
     4441 gttggaggcg cgcagaatgc ggataccacg agcgccgaca tcgcgtgcgg cggtaatatc
     4501 gttatcggaa tcgccataaa aaattcggat atttttatcc tgcagccatt gcgattttgt
     4561 attttgccct ggtttatcgc ccgcaaagat taccggattc atgttggtgg caggaatatg
     4621 aaaattatcc gccagcgttt ttgaaaccgt ttctgttttc gtcgggctac gaccagtcac
     4681 aaagaagatc gcgtcaccgc ggcgtacatg catatcaatc agctggcgag cgacctcttt
     4741 tggaatgctg aattcatccc agccattatt cattttttcc cagaacacag gatttttcag
     4801 ataatcttcg ctttctggcg agaaggtttt tttgccgcgc cagaaccccg gactggaaaa
     4861 aagtaccgtg tcatcgatat caaaccccac cgccattggc ggacgccctg cgaggctatt
     4921 ttcaatttgt gcgaccgaaa cccaatgaat tggtgcctgt tcagcaagcc tggcaacgtt
     4981 agtcccaggg ttaagcggtg aaggagatga ggccagggca acagcggaac tgtttagagc
     5041 gaacaataag caaacggcac tgattgcctg tgtgatcttg cgcatatttt tccctaaata
     5101 gtcagtatgt tgaaactttt tattgtgaga tttgttgcaa aaaccatctg accataacgc
     5161 cagctgccgg ggaaaggaag gattatctgc ggtttttgtg atgaaaagca ggatgattat
     5221 atttaaccca gtaaatacgt tcctgtcttt ttttgtataa aaaatgttta ttactcgatg
     5281 taaggataca ttaattttac gtttattatg atgaatcctc ggcaaatgag cgaaatttga
     5341 tagaacattt atctgctgta cgatttaatg aacttaatga tacagcatta caaaaacaat
     5401 cgaagtttat aaagatgatt tctgattgac cagcccccta ccctacaatg gtgattaatt
     5461 ttattactcg cgatggagat gatgatatga atatatcata tgtgaatagc aacaaaacga
     5521 catctttacc tgttgagcta gatgcactaa ataataaaga tatctcttat gccaaagatt
     5581 tctcttatgc caaagatttc tttttataca tagaaacaca gttaaaaatt gcaaaagatt
     5641 tttgtagacc tggagaagaa gtatcaagtt ctattgcaag taaagttttt cacgcattta
     5701 ttgatttagt taacaaaatc aggggtaaga aagactttat gtatatttgc acgctttgct
     5761 gttttgccga ggaggttaaa ggtgattatt ctcattacag gaccttttta tttgatattg
     5821 gtaatcaata taaggttaaa cttatacaaa gcggaaaaaa agagttctct ttaacattag
     5881 aatttaacga tactataatt gaatctcaga aagtcacagg caataaggca aagcatattc
     5941 ttgaggatat agaaatattc tatcgtaaca aacccgatac ttattattaa atttaataat
     6001 ggttagatgt gcagaaatta ttactccaca cgaaaaaata gggaaaggaa ttccagataa
     6061 ttgggataaa cttaacaata aaagaactga caacgtttta ttatctctca cgtagaacaa
     6121 cggcatcaaa aaaatcatct acaaagaaat agtgaaagcg acgacagttt aatttcactg
     6181 caggctgggt aactccagcc tgctttcctg cattacatca ccgcagcaaa cgcctttgcc
     6241 acacgttgta catttgccgt atttaacccg gcgacacaca tgcgaccgct ggcgatgaga
     6301 tagacaccaa attcttcacg tagtcggtca acctgagcgg cacttaaacc ggtataactg
     6361 aacatgccgc gctgattaag cagataatcg aaattgcgtt ctggcatctc tgtgcttaat
     6421 accttcacca attcctgacg cattgccaga atgcgagtac gcatctcttc tacttccgcc
     6481 agccagctgg ctttcaatgc ctcgtcattc agcactgcag ccaccacctg cgcaccaaaa
     6541 ttcggcgggc tggagtagtt gcggcgaact gttgctttca attgccccag tacgcggcca
     6601 gcggcttcgg catcttcaca cagaacagaa agtccgccga cgcgctcgcc gtaaagggag
     6661 aaaattttcg agaacgaatt gctcaccaga gcgggtaatc cagcgctggc aatggcgcga
     6721 atagcgtagg catcctcttc cataccggca ctaaatcctt gataggcaat atcgaggaat
     6781 gggataagct cgcgggcttt gagaatttca atcaccgcat cccactgatc attagtgaga
     6841 tcggcacccg ttgggttgtg gcaacatgga tgcagcaaca caatactgcg ggcaggtaat
     6901 gttttcagcg tcgccaacag gtcattaaag cgcacgctgt tagtcacttc gtcataccag
     6961 gggtaagtac tcacttcgaa tccagccccg gcgaatattg ctacgtggtt ttcccaagta
     7021 ggatcgctga cccagacgcc tgattccggg aagtagcgtt tcaggaaatc cgcgcccact
     7081 ttcaatgccc ctgagccgcc aagggtttga atggttgcta cgcgctgttg ttgcagtacc
     7141 ggatggtccg caccaaacag cagcggcgca atggcatggc gatagctgtt aagcccttcc
     7201 atcggtaaat aaagcgaagc gccatggggc tgcgcattca ggcgcgcttc cgcctccgcc
     7261 acggctttca attgtggaat aattccgtct tcgttgtagt acagaccgat acttaaattc
     7321 actttgtcgc tgcgagggtc ttctttaaaa cgctccataa gcgtaagaat cgggtcgcca
     7381 gcgtaggcgt caactttttg aaacacgcga tggttctcca ggtttacggg caggtggtta
     7441 aaacacaata aaccggaaga aggcgaagat cgagtggatg ttcaggagcg aacggcaatt
     7501 agcaacagag tgagactaat gataaacgta catccgccag aggcacgacc tttataagag
     7561 catgaaagaa catcaactta ttgaattttt aggattttat tggccggata aggcattcac
     7621 gccgcatccg gcacagacaa tcaaatatta cagaacgatt aatccacgta tttcatcgcg
     7681 acccttgaag tcaggcgcgt aataagttcg taagcgctta ctttcgtcat ttcagcgata
     7741 cgttctacgg gcaaaccttc gccccataaa atgaccggat ccccggcttt gtcctgcgcc
     7801 tgtggaccta agtctacgca gatcatatcc atcgccacgc gcccgacaat cggtacttcg
     7861 cgaccgttca ccagcactgg cgtaccggac ggcgcggcgc gcggataacc atcgccatag
     7921 cccatcgcga ctacgccaag acgggtatca cgttcgctta cccaggttcc accataacca
     7981 acaggctctc cggctttatg ctcacgcacg gcaatcaggc tggaggttag tgacatcact
     8041 ggctgacagc caaaatcggc accggtggag cgatcttcca gcggcgagac gccataaaga
     8101 atgatgcccg ggcgcaccca gtcaaaatgc gactgtggcc acagcagaat gccacccgac
     8161 gcggcaatgg aacgttgacc aggtttgcct tcgcaaaagg tattaaagat agcgagttgt
     8221 ttctcggttg cgccacattt tggttcatcc gcgcgcgcaa aatggctgac gatattcacc
     8281 ggctgacgaa cgtttttgca ctgggtcagg cgatgataaa acgcctcagc ctgttccggc
     8341 cttacgccca gacggtgcat accggtatcg agtttcatcc agacggtaac cggctcgtcc
     8401 aagctagcct cttccagcgc agccagctgt tcttcgttat gcacggcggt atgaaaatgt
     8461 tgcgcagaaa tcgtcggcag atctctggca tcaaaaaagc cttcgagtaa cagtacaggt
     8521 ttggtgattc cccccgcacg cagtcgcaga gcttcttcga gacgggctac gccaaaggcg
     8581 tcagcatcgg ggagcgttcg cgcggtctca agaagaccgt gaccataagc gttcgctttc
     8641 accaccgcaa ccattttact ggcaggtgcc agttcacgaa gacgttgcag gttgtgtcgc
     8701 agagcgcggc ggttaatcac aacagttgcc gcttgcattt gtgttccttg ataattgttt
     8761 gctttaatta cctaattcat aaaataatta ttattcgtcg tcgtactgcg gccccgcata
     8821 gttgtcgaag cgcgaccatt gaccgttaaa ggtcaggcgt accgtcccga ttgggccgtt
     8881 acgttgttta ccgataataa tttccgcgat gccttttaaa tcactgtttt cgtgatacac
     8941 ctcatcacga tagataaaca tgatcaagtc cgcatcctgc tcgatagagc cagattcacg
     9001 caggtcggag ttgaccggac gtttgtcggc acgttgttcc agagaacggt tcaactggga
     9061 cagcgccacc accggcacgt tcagttcttt cgccagtgct ttcagcgagc gggagatttc
     9121 tgcaatttcc agcgtacggt tatcggaaag cgccggtacg cgcatcagtt gcaggtagtc
     9181 gatcatgata agcccgatgc cgccgtgttc acgggcaata cggcgtgcgc gggaacgcac
     9241 ttccgttggc gtcaggccgg aagagtcatc gatatagata ttgcgttttt cgagcaaaat
     9301 acccatggtg ccggaaatgc gcgcccagtc ttcatcatcg agctgcccgg tacggatttt
     9361 agtctggtca acgcgcgaca gcgacgccag agaacgcatc atgatctgtt ctgatggcat
     9421 ctccagcgag aagataagta ctggtttatc ctgcaacatt gccgcgtttt cgacgaggtt
     9481 catcgcaaat gttgttttac ccatcgacgg acgcgcggcg acgatgatca aatccgacgg
     9541 ctgcaagcca gcggtttttt tgttgagatc gtcataaccg gtgtttaccc cggtaacgcc
     9601 atcgtgtggc tgctgaaaca actgctcaat acgcgccacg gttgcgtcga gcacatcggc
     9661 gatgttcttc ggcccttcgt ctttgtttgc acgactttcg gcaattttaa agacgcggga
     9721 ttcagcaagg tccagcagat cttcgctggt acgcccctgc ggatcaaaac cggcttcagc
     9781 aatctcattc gcaaccgaga tcatctcacg gacaacggca cgttcacgca cgatgtccgc
     9841 ataagcactg atgttcgccg cacttggcgt attttttgac agctctgcca gataagcaaa
     9901 accaccgacg ctatcgagtt gcccctggcg ttccaacgat tccgcaaggg taatcagatc
     9961 gataggacta ccgctttcct gcaaacgcgc catttcagta aagatatgac ggtgtgggcg
    10021 ggtgtaaaaa tcgtctgcta ccacacgctc ggctacatca tcccagcgtt cgttatccag
    10081 cattaaaccg cccaacaccg actgctccgc ttcgatcgag tgcggaggca ctttcagccc
    10141 ggcaacttgt ggatcgcgtt cgcggggttc agcctgctgt ttgttgaagg gtttatttcc
    10201 tgccatagtg aatggagtta ccgaaataaa gaatgggtcg aaactttacc atatgaagca
    10261 gaccctgacg atacgttctg gaggacacat ggcaacacga attgaatttc acaagcacgg
    10321 tggcccggaa gtacttcaag ccgtagagtt cactcctgcc gatccggcgg agaatgaaat
    10381 ccaggtcgaa aataaagcca tcggcatcaa ttttatcgac acgtatatcc gcagcggcct
    10441 ttacccgccg ccatcgctac ccagcggatt aggtaccgaa gcggcaggca tcgtgagtaa
    10501 agtcggcagt ggtgtaaagc atattaaggc tggcgatcat gtagtctatg cgcagtcggc
    10561 gttaggcgct tacagctctg tgcataacat taatgcggat aaagcggcga ttctgcctgc
    10621 ggcaatttct tttgagcaac ctgcggcatc cttcctgaaa ggcttaacgg tttattatct
    10681 gctgcgcaaa acctatgaaa ttaaacccga tgaacagttc ctgttccacg cagcggctgg
    10741 cggcgttggc ttaattgcct gccagtgggc aaaagccctg ggcgcgaaac tcatcggcac
    10801 cgtaggaacc gcgcaaaaag cgcagagcgc gctaaaagcc ggcgcgtggc aggttattaa
    10861 ctatcgtgaa gagaatctgg tcgagcgatt gaaagagatc accggcggta agaaagtgcg
    10921 cgtagtgtac gattccgtgg gcagagacac ctgggaacgg tcgctggatt gcctgcaacg
    10981 ccgcggctta atggtcagtt ttggcaactc atcaggtgcg gttaccggtg tgaacttagg
    11041 cattctcaat caaaaaggct cgttgtatgt gacacgccct tccctgcaag gctatatcac
    11101 cacgcgggag gaattaaccg aggccagtaa tgaactgttc tctttgattg ccagcggtgt
    11161 gattaaggtc gatgtcgccg agcagcagaa atatccgctg aaggatgcgc agcgtgcgca
    11221 tgagattctg gaaagccggg cgacgcaagg ttccagcctg ctgattccat aaaagaaata
    11281 gggcttccac ctgggaagcc ctttcttttt atagttcggc tgtatgtagg gtacagcacg
    11341 atgaatctgt tagaggcgca atagtgacag atttgattat caattcctat tttgttctaa
    11401 ggataaaacc ttaggttgtg atcatccgca caatccctta gtaacgccag cggtcataac
    11461 gctgatattt cggcattttt ggtgctttaa tcgccttaat aacccacacc accgcaatcg
    11521 ccagtagtaa ccacggcagc aacttaatca tcaatgccag cataccgccg aggaacataa
    11581 tggccgtcgc cacaaccagc gcggcgataa tgcccagcaa cgaaacgccg gtgaccatca
    11641 gcatgacaaa aaagccaatc acaaaaagta gttccagcat gatgctctcc caaatatgaa
    11701 atctcttgct ggcattacaa gaatcatgcc aaaaataatc tattgattta acagcaaaac
    11761 gccccgcgac ggtgcgcagg gcgtggtgaa tttgactact ttttggtgaa aagttaacgc
    11821 ttatccgcca ccagtttgag cgcgtgttcc agcacgttaa tgtcagcacc cgctttatgg
    11881 gcattttcac ttaaataacg ccgccactgc cgcgcgccag gaataccctg gaacaaaccc
    11941 agcatatgcc ggataatatg gccgagatac gtcccctggc tgagttcatg ctcaatgtac
    12001 ggatacatgg cacgcactac tgccaccgga tcggcatcgg tatccgagga accaaagatc
    12061 tcccggtcta ccgccgccag aatacccgga ttctgatacg cctcgcgccc gaccatcacg
    12121 ccatccatat gttgcaaatg cgctttagct tcttccagcg acttgatacc accgttaatc
    12181 gacattgtca gatgcgggaa gtcacgcttc agttgataca cacgcggata atcgagcggc
    12241 gggatttcac ggttttcttt cgggcttaac cccgaaagcc aggctttacg tgcgtggatg
    12301 atgaacatct cacactcgcc tttgccggaa acggtattga tgaaatcgca gagaaattca
    12361 tagctgtcct ggtcgtcgat gccaatacgc gttttcaccg tcaccggaat cgacaccaca
    12421 tcgcgcatcg ctttcacgca gtcggcaacc agctgcgcat tacccatcag acacgcacca
    12481 aacatgccgt tctgcacccg gtcagacggg cagccgacat tcaggttgat ctcatcatat
    12541 ccgcgcgctt ctgccagctt tgcacactgc gccagcgccg ccggatcgct accgcccagt
    12601 tgcaacgcta ccggatgttc ttcttcactg tacgccagat aatcaccttt accgtgaata
    12661 atcgcccctg tggtcaccat ttcggtataa agcaacgtat tgcgggaaag cagacgcaag
    12721 aaatagcggc aatgtctgtc cgtccagtcg agcattggtg caacactaaa ccgaccactc
    12781 cagtgaacgt cagttttttc aggcattgcg ctggtttggt tgattttttg catttcagaa
    12841 ttaccgtgca ttttcaaatg tagagattat tttatcgata tatcatgggg ttatgttatt
    12901 cagcttcact gttcaggagg ctcaatagcg gggtactata ccataacaac aggaagcgcc
    12961 tgtctcattg caaaagaaaa ttgagatcct ctcaaggcat gaagctctca cgaagtgatg
    13021 gaaataatct tattagccgt taactttgtt aaggccaatg ataaacaatc caggttcgac
    13081 gataaataaa aaatcacaca ttaaactctg gtgatatatc tccctgctaa tagcattgat
    13141 agagaaaaaa gaacccaata aatattgggt ccttttatat aatgccttcc atactattga
    13201 agaacttcac atattatttc tccgatttaa ccccgaacaa gtcataaatt aatttagaag
    13261 tgtctgtaag tattttaatc tcttcctttg aggttgggtc aaacgacttc gcgaagctaa
    13321 ttaattgtgg tgcagcatct cgcattttgc ttaaaatatc aggatcgagc gttccttcat
    13381 tcaccagatg tgacatctta tccagatagc catcaaaatt catttctccc ccatctgcca
    13441 gacgcttcat ctctcccagg tacttcttca tatcacgttg atataatttt tcaaactgag
    13501 ctttcaggta gctctcatta ttttcattaa tatacactgt ttctgataaa tctgcgtagg
    13561 cactagaata tgaaagtgaa attaacagcg aagcaaataa ttttaagctg ttgtcatcat
    13621 gttcctggca ggcattaaca aacgttaaaa aaccagaacc tattgcttga aaatgtatat
    13681 tcgctaaagg ttcattatcc ttagattcct cataaaatag ctgaaccgtg gagggaaggc
    13741 cattcttagt ttctacatca aaagtacatt gttcaatggg cattgcatct ttattatccc
    13801 caggattaca tatactatta atcgcgaaat gaaaaatagc ccggtcaaca actgatgcaa
    13861 taaagtcacg atcgtttaaa tcctcatcag tgcaatcgtc agtatactta ttgacccaca
    13921 ttttgagatc atttatttgc tcaattaaaa acacattttt ttacgtgcat gccataaatt
    13981 atgcgtgatt cgttgaataa acaaatctat ttttgcaata attatatccc gatgacgcag
    14041 cgctgcataa tcaatagatt taccatatat attatttaat ttacctttga ttgaacctct
    14101 attatccaat ttatctttgg ttgcatcttt attttcgaat tgatttgaat tgagtgtttt
    14161 attaaagtat ccagttgcac cgggtattat cttcaacatg attcatctac cttttttgtc
    14221 gtaccgtgtc ctccttataa ttcttttgcc atctggtcac tatcaaaaat cgctcataat
    14281 ttctgaacta caggtatgcg tttaaaccca gttaaataaa tagcgagtta acttgaagca
    14341 atttatttaa aaatgaatta ttaattatta tcgtgttgat aatggtacga caaggcaaca
    14401 caacacctta cctacaaaac agaaaatcaa cattacggca acaataaggg ttctcggtgt
    14461 ttttcctgga acatggtaaa gtaaggacat tcttaacccc cactttgagg tgcccgatgg
    14521 aaaagaccac aacgcaggag ttattagcgc aggctgaaaa aatctgcgcg cagcgtaatg
    14581 tgcgcctgac cccacagcgc ctggaagtgt tgcgcctgat gagtctgcag gatggcgcta
    14641 tcagcgctta tgatctgctt gatttactgc gcgaagctga accgcaagcc aagccgccaa
    14701 cggtttatcg cgcgctggat tttctgcttg agcaaggttt tgtgcataag gtggaatcca
    14761 ccaacagtta tgtgctctgt catctgttcg accagcccac gcatacgtca gccatgttta
    14821 tttgcgatcg ctgcggcgcg gtgaaagaag agtgtgcaga aggcgtggaa gacattatgc
    14881 atacgctggc ggcaaaaatg gggtttgccc tgcggcataa tgtgattgaa gcacatgggc
    14941 tctgtgcggc atgcgtggaa gtggaagcgt gtcgtcatcc tgaacagtgc cagcatgatc
    15001 actctgtgca ggtgaaaaag aaaccgcgtt aagagggcgt acatccttgt acacgtcggg
    15061 caggagggat taattaccag cgatattcat tgcgggtttc ccaatccacg acctcttttt
    15121 ctgcctgatc tttctgataa ccataacgtt cctggatttt accgaccagt tgatcacgtt
    15181 taccttcaat gatcgtcata tcatcatcgg tcagtttgcc ccattgctct ttcactttac
    15241 ctttaaactg tttccagtta ccgccggctt catctttatt cataatcaag acctcatcgt
    15301 taggttgtga atgagagtac gttcactttt cttctgaacg tgagattaag tgtagtcagc
    15361 aatttggctt aggataattt attcagaatt tttaaccgtc acgttgcggc aaaccaggta
    15421 ccgttgcgcc agtgacgccg ccagatagcc gccagagaaa gcccgcgcaa cgccagaaag
    15481 acggttaatg ccagccacaa accatgatta cccagccacg gcagcgtaag gagcgtcagc
    15541 gcaaaacctg cggcggccac cgccatactg ttacgcattt cggcggcacg cgttgcgcct
    15601 ataaacatgc cgtccagcag ataacaccag acgccaacca acggcaaaat cacctgccag
    15661 ataagatagc ggtcagccag ctgctgaatc tgggttaacg acgtcagcaa cgcaatgatg
    15721 tgttcccctg ccagcaaata aaccaccgaa aacagtaacg ctacaatccc cgactggcgg
    15781 cacgctgccc gccatacatc cagcaactgg ctaccgtcgc gcgcaccgta tgcctgaccg
    15841 gagtgcgctt caaccgcgta ggcaaaacca tcaagcgcat aggcggtaaa ggtgagtagc
    15901 gtcatcagaa ccgcgttaac agcgataatg tcactcccca gtcgcgcacc aagtacggtg
    15961 atcgcgccga aacagagttg caacaacagc gaacgcagca tgatatcgcg gttaagcgcc
    16021 agcaagcgac ggaagtttcc tcgccaggca gttttcagca tttcgccgga aattccgcgt
    16081 agtttgagga ttttacgcac cattagcaga ccaatcagca atgttgcata ttccgcaata
    16141 accgtcgcca gcgccgcgcc ctgcacgttc atatgcagcc ccatcaccag ccagacatcc
    16201 agcacaatgt tgaggatatt gccgaccact aacaaaatta ctggcgcacg ggcatattgc
    16261 acgccgagta accaaccgag taataccaga ttcgccagcg acgccggagc gcttaaccag
    16321 cggatttcaa gaaagcgccg cgcctgttcc aggaccgctt cactaccgcc aacaatatgc
    16381 agcgccagat cgataatcgg cgtacgcaac agcgcaatta acgccccggc ccccaacgcc
    16441 aacagcaacg gttgcaccag cgcacgggct aatgcctgag gatttttggc accataagcc
    16501 tgcgcagtca gcccggtggt gctcatgcgt aaaaacagca acagcataaa gagaaagctg
    16561 gtcgccgttg caccaaccgc cacgccgccc aaataaaccg gactatcaag atgaccaatt
    16621 accgccgtat cgaccagtcc cagcaacgga acggtgatat tggagaaaat catgggtaag
    16681 gcgagatgcc agagtgcttt atcagatgaa gtgaggaatg ccatgcagac aagcctgatg
    16741 aagagagatg aaaaacaaac cgcgacgcca ggcggcatcg cggtctcaga gatatgttac
    16801 agccagtcgc cgttgcgaat aaccccaacc gccagccctt caatggtgaa gctctgctga
    16861 cgaaggtcaa cgacaattgg tttaaactcg ctattttctg gcaacagttc gactttattg
    16921 ccctgttttt tcaggcgctt aacggtaact tcgtcatcaa tacgtgcgac aacgacctga
    16981 ccgttacgta catcctgagt tttatgcact gccagcaagt cgccatccat aatgccgata
    17041 tctttcatcg acatcccgct gacgcgcagc aggaaatcag cattcggctt gaacaaggaa
    17101 ggatcgacct gataatgacc ttcaatatgc tgttgcgcca gaagcggttc accggcagcc
    17161 acacgaccta ccagcggcaa cccttcttcc tcttcctgca acagacgaat cccgcgtgat
    17221 gcgccggaaa caatttcaat aacgcctttg cgtgccagcg ccttcagatg ttcttcagcc
    17281 gcgtttgggg aacggaaccc caaacgctgc gcgatttccg cacgcgtcgg cggcatacct
    17341 gtctggctga tgtgatcacg gatgagatca aacacctctt gttgcctggc cgttaacgct
    17401 ttcattccgc cccctgggtg tatatacagt tatactgtga gtatatacag caaaaggcga
    17461 ttttggaacc ataaactgca caataaacca gagatttatc gaaatctgga agggttatcc
    17521 aaaatgcgac cataacagga tgcaccaggt aatcacggcg acgataatgg caatcagcac
    17581 cgcggcggat cccatatctt ttgcgcgtcc ggaaagctca tggtattcag agccaattcg
    17641 gtcaaccact gcttcgatgg cgctattgag gatttccaca atcatcacca gcatcacgga
    17701 gctgataagc agcacgcggg taattgcgtc cacatccagc cagcaggcga tgaccaccgc
    17761 caacaatacc gctacgcctt cctgacggaa tgccgcttcg ttgatccatg cagcgcgtaa
    17821 acctttccag gaatagccag cagctttgat aattcgggtg aatccagtgg tattattggc
    17881 cattgaaaga acctttttac attatgagcg tcaatatcag tgtaccgtta aatccgatga
    17941 ttcgggtttg cgtaacgccg cgcgaaacat gagcggatac cacagaattt cccatgactt
    18001 tctgctatcc ttgccgcgca tttgcattat taaccagagg ctttacatcg tttatgtccg
    18061 gctggccacg aatttactac aaattactga atttaccatt aagcatcctg gtaaaaagca
    18121 agtctattcc ggcagatcct gccccggaac tggggctgga tacctctcgt ccaattatgt
    18181 acgttttacc gtacaactcg aaagcagatt tgctgacgtt gcgcgcccag tgtctggcac
    18241 atgatttgcc tgacccgtta gagccgctgg aaatcgacgg cacgctactg ccgcgctatg
    18301 tgttcattca cggcgggccg cgtgtattca cctattacac gccgaaagaa gagtctatta
    18361 agcttttcca cgactatctc gatttgcacc gtagcaaccc aaatctggat gtgcagatgg
    18421 tgccagtatc ggtgatgttt ggtcgcgcgc cggggcgtga aaaaggcgaa gtgaacccgc
    18481 cgctgcgtat gcttaacggc gtacagaaat ttttcgctgt actgtggctc ggtcgcgaca
    18541 gttttgtgcg tttctcgccg tcagtgtcgc tgcgccgtat ggcggatgaa cacggcacgg
    18601 ataaaactat cgctcagaaa ctggcgcgcg tggcgcgtat gcactttgcc cgtcaacgtc
    18661 tggctgccgt aggcccacgt ctgcctgctc gtcaggatct gtttaataag ctgctcgcct
    18721 cccgcgccat tgccaaagcg gtagaagatg aagcgcgcag caaaaaaatc tcccatgaaa
    18781 aagcgcagca gaacgcgatt gcgctgatgg aagagattgc ggcgaatttc tcttacgaga
    18841 tgatccgcct gactgaccgc attctgggct tcacctggaa ccgattttat cagggcatca
    18901 acgtccataa cgccgagcgc gttcgccagc tggcccacga cggtcatgag ctggtgtatg
    18961 tgccttgcca ccgcagtcac atggactacc tgctactttc ttacgtgctg taccaccagg
    19021 ggctggtgcc gccgcatatc gccgccggga tcaacctgaa cttctggccg gccgggccga
    19081 ttttccgccg tctgggggcg ttctttattc gccgtacttt taaaggcaat aaactttatt
    19141 ccaccgtttt ccgtgagtat ctcggcgaac tgttcagccg tggttattcc gtcgagtact
    19201 tcgtggaagg cggtcgttcc cgcacggggc gtttgctgga tccgaaaacc ggtacgctgt
    19261 cgatgaccat tcaggcgatg ctgcgtggcg gcacgcgtcc gattacgctg attccgatct
    19321 atatcggtta tgagcacgtc atggaagtgg gtacttacgc caaagaactg cgcggcgcga
    19381 cgaaagagaa agagagcctg ccgcagatgc tgcgcggttt aagcaagctg cgtaatctcg
    19441 gtcagggtta cgtcaacttc ggtgaaccaa tgccgttgat gacctacctt aaccagcacg
    19501 taccagactg gcgtgaatct atcgatccca tcgaagcggt gcgtccggcc tggttaacgc
    19561 cgacggtcaa taatattgct gccgatctga tggtacgcat taacaacgca ggcgcggcaa
    19621 acgccatgaa cctgtgctgt actgcgctac tggcatcacg tcagcgctca ctcacccgcg
    19681 agcagttaac cgagcaactc aactgctacc tggatctgat gcgcaacgta ccttactcca
    19741 cggactctac cgttccttca gccagcgcca gcgagcttat cgatcacgcg ctgcaaatga
    19801 acaagtttga agtcgagaaa gacactatcg gcgacatcat cattctgcca cgcgagcaag
    19861 cggtgctgat gacctactat cgcaacaaca ttgcgcatat gttggtgctg ccttcgctga
    19921 tggcggcaat tgtcacccag catcgccaca tctcccgcga cgtattgatg gagcacgtca
    19981 atgtgcttta cccaatgctg aaagcggagc tgttcctgcg ctgggatcgc gacgagttgc
    20041 cggacgttat tgatgcgctg gcaaatgaga tgcaacgtca ggggctgatt accctgcaag
    20101 atgatgagtt gcatatcaac ccggcgcatt ctcgcaccct acagctgctg gccgcaggcg
    20161 cgcgcgaaac gctgcaacgt tatgccatca ccttctggtt gttgagtgcc aacccgtcga
    20221 tcaaccgcgg tacgctggag aaagagagcc gcaccgttgc gcaacgtctc tccgtgctgc
    20281 acggcatcaa cgcgccggag ttcttcgaca aggcggtgtt cagttctctg gtgctgacgc
    20341 tgcgtgatga agggtatatc agcgatagcg gcgatgccga accagcagaa acgatgaagg
    20401 tttatcagtt gctggcggag ttgattacat cagacgtgcg tttgacgatt gagagtgcga
    20461 cgcagggcga agggtaatca gagagaattg ccggatgcgg cgaaaacgcc ttatccggcc
    20521 taccatgacc tgcaaattca ataaattgcg attcaccggg taggcctgag aagcgcagcg
    20581 catcaggcaa cccagaagaa agccggatga tcatccggct tttttacatc atcagaaatg
    20641 ccagtaactc attgccagtc ctaaaaacag taccagacca acatagttat tattcataaa
    20701 tgctttaaag caggcttcac gctcgcggtt ggcaatcagt ttttgttgat aaacaaacag
    20761 cgcgccagcc accacaattg accagtaata tccccagcct aagccattta actcaccgat
    20821 gatcgccatc agtgccagta cgccaatctg caaaatacca ataatcaatt tatcgtattg
    20881 gccgaacagg attgccgtgg atttaatgcc aatcttcaca tcatcatcgc ggtcaaccat
    20941 cgcatactgc gtgtcgtaag ccaccgccca gagaatattg gcgaggaaca ttaaccagca
    21001 actcaatggc accgactcac tcacagcggc aaaagccatt ggaatcgacc agccaaacgc
    21061 cgcgcccagc accacttgcg ggagatgggt ataccgcttc ataaacgggt acacccacgc
    21121 caatgccaga gcggcaatcg acaacagaat ggtcatcgta ttcagcgtca gaaccagtaa
    21181 aaacgaaatc agtaccagca ctacaaacag cgcgcgcgcc tctttctctg ttaccgcgcc
    21241 gctgggaagt ggtcggttcg ccgtgcgctt aacatgacca tcaaacttgc gatcagcata
    21301 atcattcacc acgcaaccag cagcgcgcat cagccagaca cccgcgacaa acaccgccag
    21361 gatccagagc tgaggaacgc ccggtgtcgc cacccacaac gcccataatg ttggccagag
    21421 cagcaataac gcgccaattg gcttatccgt acgcattaag cgatgaaacg ccagcagctt
    21481 attctgcgtc agactccact ccatattttt ttcctcttag tacaacggtg acgccggtaa
    21541 aaacagttct gttagcaaca gcggtttacc gcttaatcgc aggcgggaac gtcgccccca
    21601 cagtccggca tcacggccta tctcgataaa atcccgggtt agtgtcgatg atgtgaacag
    21661 atagcgtcct aacggcgttt tacccaattt ttgtaacgct agttccggcc cgcttaatgt
    21721 tgatacagga acgacggtac gaccggcaag ccacggttca ccatcggcac ataacaaaat
    21781 ttcacgtaac cagtaacgag actctttcgg cagtagcggc agttcttcgg ggatttcatt
    21841 ctgctcgaca aacccctcgc ggatcatcgt cacactcacc gtttttccct gctgttcaaa
    21901 acgttttgtc atggaatcct ccagcaacag ccagtcgaac agttgcggat ccagggcagg
    21961 gatctcttta aaatagcgca gcgcacgcag ttgcgttaac gcggggtgtg acatgccgaa
    22021 ctctccgtta cataacgtaa aggcattgta tcgcagaaaa gtgtttacgg gagatgtaaa
    22081 gcaaattaag tgatctaatg cgcaacaaaa acgcaacaat cgcatgaagt ggtaaaacgc
    22141 tgttgaacct tacgctttgc cgatgcccgt gctggcgagg gcaaccatag gggcatcacc
    22201 gcccatgatg ctaggaacgg tgatgttttc ctttacttaa atacctttat tctttatacg
    22261 agtagctcgc caactcgcag taaaaattgg gaaaagcgca tatttaccat gagatttgga
    22321 gctgaaaagt gtttcctgag taagattaat aggcaacatc ttatgtctgg atttatagat
    22381 acacgtggaa ttccaattgg tatcttttgt aggattcaac atatcatgta agcgctggga
    22441 gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata tattatcgtt
    22501 tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta taaatatagg
    22561 tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg ctttgatcat
    22621 atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt tatttttatc
    22681 aaccagccct ttattcatat taaatctgtt aataaaatgg atcatctgtt gggtatcgaa
    22741 attcacctta ctgaatcgat gttgacgcat aaaattatcg aacttgtcat cttccccaga
    22801 atgtatataa ctacgtataa ttggatcttt tatataagga caatggctaa atgttttctt
    22861 catattctca ataaagtttt tattaacaat attgagaatt tttaattgca tcaccagttt
    22921 tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa tcatttttat
    22981 aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat aatcaatagt
    23041 taattccaat tgttctgtaa tgcaatctta aaaattccct gtcgacattc gcacatgatc
    23101 aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag acagaaactc
    23161 tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat ttctaatctg
    23221 acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg tatcaagaat
    23281 attttggcta aaatcaacac attcgccact cgttttgaga aaaatcacat tgtgtaaacg
    23341 tcgacattta aatatgacat tatgaactgt tgccttagta aaattgcaat tctcaatttc
    23401 tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct tagaaataac
    23461 gtcaccttta aaatgacaat ttgttaaatc aaaatcagac aggtcgatat acccctgcgc
    23521 atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt agaattttac
    23581 acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat gatttttttc
    23641 gttccgttgt tttatattta aaaatatatc actgatataa ataaagttat ctctgtttaa
    23701 acctgtcacc ccattgaaat tacgtagtaa atcaagatct actatattat ttccttgcac
    23761 cacatcatgt ttcatcaaag agttattgcc gccagaattt gtgatatgac ctatcataaa
    23821 acctctgtaa tacagaaata aaataacctg gaaaagtagc ctgagcgtcc agacactacc
    23881 ggcgaaagga aaagaatctc tgataatgca ttgaaataat ggacattctt agtgaagaag
    23941 aagtgaagta ctataaaaaa tcgctcatat cccaggaagg aagcataaaa aaaggtgcgc
    24001 caggagacgc accagttgtt gcaaaatcag cactgtgggg cgtaattacc ccttgccttt
    24061 tacactgctg ataaaggtgg aacgggcaga tgtcgatccc aggcgttcag cttcatcaag
    24121 cagttttaac gccttatcaa catcaccttt cgcgacagcg tttttgatcg cggtattaaa
    24181 ataactttcc gtgtcgttga gcatcggctc gcttttcttc accggcaccg gagcaggtgc
    24241 agccacagct ggtgccgccg tgttgcctac cgtaaccgga gctggagcgg aagaaccaaa
    24301 tagtggtcct accaacacgc tggagctgga gttcgttttg actttcagtt tcagtaagcc
    24361 atcgctggta tgacgagcaa ccggatcggg aatatccggg atcgagttac cgacgccttt
    24421 ggcataggct ttagccgggt cgagcagttg ggtcgtctgc tggagatctt tttccatggt
    24481 aaagaccaga acataaagtt tttgctgccc caacgccggt gtcaggcgca taacgccttc
    24541 cagccgatct gcactcatca cgcctagttc ctggtaggtg aaataactgc tggggaagaa
    24601 ggctgatggg gtcatgttct gatcaataat cagcacgttc ggcgcaaaaa cgctggtttg
    24661 tttgttcact tcgctggtca gcgtcagggt cagttcgcca atgtttgccg ggacgctgta
    24721 cgcagcaacc ggaccactga tgccgggaac gctcagttgt tggccgccgg tcgccagttg
    24781 ggtggtctgg gttttagatt gatcgaccgg tgtccaggtg agttgttgca gcgcggcaga
    24841 tggaatggct ggcgcgtcgc tggtgttttg cggtacgtag ttaacatcgg caaggctaat
    24901 tccaggcgcg cttgccagta accctgctga taaacagagg gcgatgagac ttttattcat
    24961 tttcattgtt ttcacctcaa aatctggagc tcagcggtag ccaggcaata gcgcgctaaa
    25021 cccgataatc agaggggctt tcgccccttt cagataatga caacctgttt ttatgccgga
    25081 tgcggcgtaa acgccttatc cggcccaggt tttgctatta ccaccagatt tccatctggg
    25141 caccgaaggt ccactcgtcg ctgtcgccac gaccgaagct gccgccgttg aaatcagcag
    25201 gaacggcttt gccgtagttc gggttaacct tgctatcgcc gttgtagtcg taaccccatt
    25261 tctcatccca cttggcgtag gttgcgaaga cacgaatagc cgggcgtgac cagatgctgt
    25321 cgccagcctg ccattgttgt gcgagggtaa ttttgtactg attgttcttg tcgccggtgc
    25381 gctgggattc gacgttgtcg tagccgattt ccatcacggt gctcatgatt ggcgtccact
    25441 tgtacatcgg gcgaatacca acggtccacc acttggtgcc gttgtcgtta tcccagttga
    25501 tatcctggta catacccacg tacatcatgt cccagttgtc gcccatggag atcgcaccgt
    25561 ggtcgaggat acgcagcatg tgaccgttgt tgttgatatt gtaggcaaat ttttcgttat
    25621 caaacgcgac gccagaaccc tgcgacagac ctttaccctg cgaggtcatc gagtcagtag
    25681 cgtactgaac aacaaacttg ttaaagccct tcaggacact ctgagtatgt tcagcagtga
    25741 ataaccagcc gtctttcgat gcgccatcaa ccagacgata gttatcacgc aggttggcac
    25801 gaccgtagtc gacacccagt tctaatgtgc cgcccgggtt gatttccatc tgcgctaaac
    25861 gcacatcgaa aacgtcgttc gcggtttcgt tggtatagtc ataaatattg ttgctggcaa
    25921 aagaggaaga accaccagct tcagaggagc gggttgctgc cagagagagt ttaccgaagc
    25981 caacatcgat gttttccaga ccggcaccag gaccagaaat atcccagtag tagaagtcga
    26041 tcatatgaac gtcatgacgt tggtagaagc gcttacctgc ccagatggtg gaacctggca
    26101 gccattcgat caggtttttt ccctgcacgt ttgcttcacg gaaggccgga tcggtagctt
    26161 cccagtcatt ctgttgcgcg acggaatagg ccacgttagt gtcgaaatag aagctcttat
    26221 cgccctcttt ccacacttcc tgacccaatt ttaattcagc ataagtttca cattcgttgc
    26281 caagacggta tttactttga gcaccggtag tctggaaaca ctgttgttca ccgccgctac
    26341 ctgtccagcc aataccggaa cgtgcatagc cgtggaaatc aacagccatt gcctgagcag
    26401 acattacgcc cgctgcgacg gcaaccgcca gaggaagttt gcgcagagta atcatcattc
    26461 tatctcctga gtcattgctt ttcttttttc acatcacctg tgacaggctt tgtgtgtttt
    26521 gtggggtgct taaacacccg gctccttatg cagtcgacga catgcagtgc catcctcacg
    26581 gaacagatgg caacgctctg gcggcaggcc gatagcgaat gtggcacctt cttctaccaa
    26641 caccacgtcg ttctggcggt acaccaggtt ttgacgaatg gaagggatct ggatatagat
    26701 ttgggtttcg ttgccgagtt gctcgacgac ctgaacttca ccctcaagga tgacgtcagc
    26761 gatatcactc ggcagtagat gttccgggcg aatacccagc gacatattgg ctccaacctg
    26821 gacatcacgg ctttcaacag gcagccagac ttgttgacga tttggcatcg gcagctccac
    26881 ctgcacttga tcgattgcgg tggcggtcac ttttaccggc aggaagttca tctttggcga
    26941 accgataaat ccggcgacaa aacggtctgc cggatagtgg tacagttcca gcggtttccc
    27001 aacctgtgcc acgcgaccgg cgtccagcac cacgattttg tcggccagcg tcatcgcttc
    27061 gacctgatcg tgggtgacgt aaatcattgt gcggcccagg cgtttatgca gacgggagat
    27121 ttcgatacgc atttgcacac gcagtgcagc atcgaggttg gagagcggtt catcgagcaa
    27181 aaatacgctt ggctcggcca ccagcgtacg gccaatcgcc acgcgctgac gctgaccacc
    27241 ggagagcgct ttcggtttgc gatccagcaa atgcgccagt tgcagcactt ccgccacctg
    27301 gttgacgcgt tggttaatca cctctttttt tgcgccagcc agtttcaggc caaatgacat
    27361 gttttctgct actgacaggt ggggatagag cgcgtaagac tgaaacacca taccaacgcc
    27421 gcgttctgct ggcggagtgt cattcatccg tttctcaccg atgaacaggt cgccgctggt
    27481 gatcgtctca agcccggcaa tcatgcgcag taaagtcgat ttaccgcagc cagacggtcc
    27541 gacaaacacc acgaattcac cttcatggat atcgagattg atatctttcg ataccacgac
    27601 ctcgccccag gctttcgtta cattttgcag ctgtacgctc gccatgccct tctccctttg
    27661 taacaacctg tcatcgacag caacattcat gatgggctga ctatgcgtca tcaggagatg
    27721 gcttaaatcc tccaccccct ggctttttta tgggggagga ggcgggagga tgagaacgcg
    27781 gcttctgtga actaaaccga ggtcatgtaa ggaatttcgt gatgttgctt gcaaaaatcg
    27841 tggcgatttt atgtgcgcat ccccacatta ccgccaattc tgtaacagag atcacacaaa
    27901 gcaacggtgg ggcgtagggg caaggaggat ggaaagaggt tgccgtataa agaaactaga
    27961 gtccgtttag gtgttttcac gagcacttca ccaacaagga ccatagatta tgaaaataaa
    28021 aacaggtgca cgcatcctcg cattatccgc attaacgacg atgatgtttt ccgcctcggc
    28081 tctcgccaaa atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa
    28141 cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga
    28201 gcatccggat aaactggaag agaaattccc tcaggttgcg gcaactggcg atggccctga
    28261 cattatcttc tgggcgcacg accgctttgg tggctacgct caatctggcc tgttggctga
    28321 aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacatggg atgccgtacg
    28381 ttacaacggc aagctgattg cttacccaat cgctgttgaa gcgttatcgc tgatttataa
    28441 caaagacctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga
    28501 actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg
    28561 gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat
    28621 taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct
    28681 gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa
    28741 taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag
    28801 caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ctaaaccgtt
    28861 cgttggcgtg ctgagcaccg gtattaacgc cgccagtccg aacaaagagc tggcgaaaga
    28921 gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc
    28981 gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc
    29041 cgccaccatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc
    29101 tttctggtat gccgtacgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga
    29161 tgaagccctg aaagacgcgc agactcgtat caccaagtaa tgctgtgaaa tgccggatgc
    29221 ggcgtgaacg ccttgtccgg cctacaaaac tgaaacgcat gtaggcctga taagacgcat
    29281 cagtgtcgca tcaggcagtt gttgtcggat aaggcgtgaa agccttatcc gtcctggaat
    29341 gaggaagaac cccatggatg tcattaaaaa gaaacattgg tggcaaagcg acgcgctgaa
    29401 atggtcagtg ccaggtctgc tcggcctgct ggtgggttac cttgttgttt taatgtacgc
    29461 acaaggggaa tacctgttcg ccattaccac gctgatattg agttcagcgg ggctgtatat
    29521 tttcgccaat cgtaaagcct acgcctggcg ctatgtttac ccgggaatgg ctggaatggg
    29581 attattcgtc ctcttccctc tggtctgcac catcgccatt gccttcacca actacagcag
    29641 cactaaccag ctgacttttg aacgtgcgca ggaagtgttg ttagatcgct cctggcaagc
    29701 aggcaaaacc tataactttg gtctttaccc ggcgggcgat gagtggcaac tggcgctcag
    29761 cgacggcgaa accggcaaaa attacctctc cgacgcgttt aaatttggcg gcgagcaaaa
    29821 actgcaactg aaagaaacga ccgcccagcc cgaaggcgaa cgcgcgaatc tgcgcgtgat
    29881 tacccagaat cgtcaggcgc tgagtgacat taccaccatt ctgccggatg gcaacaaagt
    29941 gatgatgagc tccctgcgcc agttttctgg cacgcagccg ctctacacac tcgacggtga
    30001 cggcacgttg acgaataatc agagtggcgt gaaatatcgt ccgaataacc aaattggctt
    30061 ttaccagtcc attaccgccg acggcaactg gggggatgaa aagctaagcc ccggttacac
    30121 cgtgaccacc ggctggaaaa actttacccg cgtctttacc gacgaaggca ttcagaaacc
    30181 gttcctcgcc attttcgtct ggaccgtggt gttctcgctg atcactgtct ttttaacggt
    30241 ggcggtcggc atggttctgg catgtctggt gcagtgggaa gcgttgcgcg gcaaagcggt
    30301 ctatcgcgtc ctgctgattc tgccctacgc ggtgccatcg ttcatttcaa tcttgatttt
    30361 caaaggattg tttaaccaga gcttcggtga aatcaacatg atgttgagcg cgctgtttgg
    30421 cgtgaagccc gcctggttca gcgatccgac caccgctcgc acgatgctga ttatcgtcaa
    30481 tacctggctg ggttatccgt acatgatgat cctctgcatg ggcttgctga aagcgattcc
    30541 ggacgatttg tatgaagcct cagcaatgga tggcgcaggt ccgttccaga acttctttaa
    30601 gattacgctg ccgctgctga ttaaaccgct gacgccgctg atgatcgcca gcttcgcctt
    30661 taactttaac aacttcgtgc tgattcagct gttaaccaac ggcggcccgg atcgtcttgg
    30721 cacgaccacg ccagccggtt ataccgacct gcttgttaac tacacctacc gtatcgcttt
    30781 tgaaggcggc gggggtcagg acttcggtct ggcggcagca attgccacgc tgatcttcct
    30841 gctggtgggt gcgctggcga tagtgaacct gaaagccacg cgaatgaagt ttgattaagg
    30901 gagataacaa aaatggcaat ggtccaaccg aaatcgcaaa aagcacgttt gtttattact
    30961 cacctgctac tgctactttt tatcgcggcg attatgttcc cgctgctgat ggtcgtcgct
    31021 atctcgctgc gtcagggaaa cttcgcgacc ggtagcctga tcccggagca aatctcctgg
    31081 gatcactgga aactggcgtt aggttttagc gttgaacagg ctgatggtcg cattacgcca
    31141 ccgccattcc cggtactgct gtggctgtgg aactcggtaa aggtcgctgg gatttccgcg
    31201 attggcattg tggcgctctc caccacctgc gcctacgctt tcgcccgtat gcgctttcca
    31261 ggcaaagcga cgctgctgaa aggaatgctg attttccaga tgttcccggc agtactttca
    31321 ctggtcgcgt tgtatgcgtt gtttgatcgt ctgggtgagt acattccatt cattggcctg
    31381 aatactcacg gcggcgtaat tttcgcgtat ctgggtggga ttgcgctgca tgtctggacc
    31441 atcaaaggct atttcgaaac catcgacagt tcgctggaag aagcggctgc gctggatggt
    31501 gcgacaccct ggcaggcctt ccgccttgtc ctgttgccgc tgtcagtacc gattctggcg
    31561 gtggtattca tcctgtcgtt tatcgctgcc attactgaag ttccggtcgc gtcgctgtta
    31621 ctgcgtgacg taaacagcta caccctggcc gtggggatgc agcaatacct caacccgcaa
    31681 aactacctgt ggggtgactt tgccgccgct gccgtgatgt ctgcattacc gatcaccatc
    31741 gtcttcttgc tggctcaacg ctggctggtc aacggcctga cggcaggtgg tgtgaaaggt
    31801 taaagatgtt gttctgccaa tgttatgccg ctgcaccctc aacttacgtt atcccaactt
    31861 gtgactatta ttcggcgctc cacggagcgc cttttttctt tcgtctgcaa tctgaatcgt
    31921 tcgccggtta atattcccat catagaactt attattttta cgttatttgt tttcccactt
    31981 acgataattc tctttcgtgc tctgagtcac ggcaatagta ttgttttcat caatttttga
    32041 taattatcac aattaagatc acagaaaaga cattacg
//
LOCUS       DAJTYF010000035        32003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTYF010000035 DAJTYF010000000
VERSION     DAJTYF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.035"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..695
                     /locus_tag="OO590_001736"
     CDS             <1..695
                     /locus_tag="OO590_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6637131.1"
                     /translation="GQHYYIYDTKFLTLYLEQTEMKNLPIGGVWKGKVKLHSNSPAQD
                     YFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDG
                     FNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQI
                     DVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKL
                     SVIFTPSLPTIN"
     gene            complement(783..2081)
                     /gene="exuT"
                     /locus_tag="OO590_001737"
     CDS             complement(783..2081)
                     /gene="exuT"
                     /locus_tag="OO590_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6637132.1"
                     /translation="MSKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            2564..3976
                     /gene="uxaC"
                     /locus_tag="OO590_001738"
     CDS             2564..3976
                     /gene="uxaC"
                     /locus_tag="OO590_001738"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6637133.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHFELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            3991..5478
                     /gene="uxaA"
                     /locus_tag="OO590_001739"
     CDS             3991..5478
                     /gene="uxaA"
                     /locus_tag="OO590_001739"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6637134.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            5561..6112
                     /locus_tag="OO590_001740"
     CDS             5561..6112
                     /locus_tag="OO590_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6637135.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(6117..7361)
                     /gene="sstT"
                     /locus_tag="OO590_001741"
     CDS             complement(6117..7361)
                     /gene="sstT"
                     /locus_tag="OO590_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6637136.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(7957..8922)
                     /gene="alx"
                     /locus_tag="OO590_001742"
     CDS             complement(7957..8922)
                     /gene="alx"
                     /locus_tag="OO590_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6637137.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(9193..9393)
                     /locus_tag="OO590_001743"
     CDS             complement(9193..9393)
                     /locus_tag="OO590_001743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6637138.1"
                     /translation="MQDLTQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLL
                     TEIRRQTGVIFPADSVKLYLPK"
     gene            complement(9424..10191)
                     /locus_tag="OO590_001744"
     CDS             complement(9424..10191)
                     /locus_tag="OO590_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6637139.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSEC"
     gene            complement(10270..10962)
                     /locus_tag="OO590_001745"
     CDS             complement(10270..10962)
                     /locus_tag="OO590_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6637140.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(11039..11542)
                     /locus_tag="OO590_001746"
     CDS             complement(11039..11542)
                     /locus_tag="OO590_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6637141.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            11627..12763
                     /gene="rlmG"
                     /locus_tag="OO590_001747"
     CDS             11627..12763
                     /gene="rlmG"
                     /locus_tag="OO590_001747"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6637142.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            13048..13362
                     /gene="higB"
                     /locus_tag="OO590_001748"
     CDS             13048..13362
                     /gene="higB"
                     /locus_tag="OO590_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6637143.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            13359..13775
                     /gene="higA"
                     /locus_tag="OO590_001749"
     CDS             13359..13775
                     /gene="higA"
                     /locus_tag="OO590_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6637144.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(13820..15838)
                     /gene="fadH"
                     /locus_tag="OO590_001750"
     CDS             complement(13820..15838)
                     /gene="fadH"
                     /locus_tag="OO590_001750"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6637145.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16058..18409)
                     /gene="ygjK"
                     /locus_tag="OO590_001751"
                     /pseudo
     CDS             complement(16058..18409)
                     /gene="ygjK"
                     /locus_tag="OO590_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(18426..18845)
                     /locus_tag="OO590_001752"
     CDS             complement(18426..18845)
                     /locus_tag="OO590_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637146.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(18823..19176)
                     /locus_tag="OO590_001753"
     CDS             complement(18823..19176)
                     /locus_tag="OO590_001753"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637147.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(19177..19497)
                     /locus_tag="OO590_001754"
     CDS             complement(19177..19497)
                     /locus_tag="OO590_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637148.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(19631..21064)
                     /locus_tag="OO590_001755"
     CDS             complement(19631..21064)
                     /locus_tag="OO590_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6637149.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(21127..21576)
                     /locus_tag="OO590_001756"
     CDS             complement(21127..21576)
                     /locus_tag="OO590_001756"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6637150.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATHALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(21573..24659)
                     /gene="ebgA"
                     /locus_tag="OO590_001757"
     CDS             complement(21573..24659)
                     /gene="ebgA"
                     /locus_tag="OO590_001757"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6637151.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(24842..25825)
                     /gene="ebgR"
                     /locus_tag="OO590_001758"
     CDS             complement(24842..25825)
                     /gene="ebgR"
                     /locus_tag="OO590_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6637152.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            26044..26375
                     /locus_tag="OO590_001759"
                     /pseudo
     CDS             26044..26375
                     /locus_tag="OO590_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(26417..27796)
                     /gene="ygjG"
                     /locus_tag="OO590_001760"
     CDS             complement(26417..27796)
                     /gene="ygjG"
                     /locus_tag="OO590_001760"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6637153.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            28213..29733
                     /gene="aer"
                     /locus_tag="OO590_001761"
     CDS             28213..29733
                     /gene="aer"
                     /locus_tag="OO590_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR6637154.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(29887..30510)
                     /gene="nfeR"
                     /locus_tag="OO590_001762"
                     /gene_synonym="yqjI"
     CDS             complement(29887..30510)
                     /gene="nfeR"
                     /locus_tag="OO590_001762"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6637155.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            30787..31533
                     /gene="nfeF"
                     /locus_tag="OO590_001763"
                     /pseudo
     CDS             30787..31533
                     /gene="nfeF"
                     /locus_tag="OO590_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(31587..31662)
                     /locus_tag="OO590_001764"
     tRNA            complement(31587..31662)
                     /locus_tag="OO590_001764"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31627..31629),aa:Ile,seq:cat)
     gene            31787..>32003
                     /gene="mug"
                     /locus_tag="OO590_001765"
     CDS             31787..>32003
                     /gene="mug"
                     /locus_tag="OO590_001765"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR6637156.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDR"
BASE COUNT         7731 a         8361 c         8405 g         7506 t
ORIGIN      
        1 atggtcaaca ttattatata tacgatacaa agtttcttac gctctacctt gagcagacag
       61 agatgaagaa tttgccgatt ggtggcgtct ggaaggggaa agttaaatta cattcgaaca
      121 gcccggccca ggactatttc gcaaatatta ccctgaatac gctcgacccc aaccatattg
      181 acgtgttctt cccggagttc gcccacgcca cgccaagggt gcagttagac ttgcatccaa
      241 caggaagcgt taacggcagc aactacgcgc aagatctgac catgttggac atgtgcctgt
      301 acgatggttt taacggtaat gccatcagtt atgaaatcat gctcaaagat gaagggcgac
      361 cagctgcagg gcgcagagac ggttacttct ctatctatcg tcagggaggg accaccaccg
      421 acgagggaga acgcattgat taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa
      481 ttgatgtgcg caataatgaa aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc
      541 cggtggtact gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg
      601 cagtcgataa attcagcgtg atggataaac aggccgggta ctacatgggc aaattgtcag
      661 taatctttac gccttccttg ccaaccatca attaaccctg caaaccggac aaacgctttt
      721 ttctatataa acgacgaagc cgcctgataa ggcggcttcg tcgttataac ttacgttaaa
      781 cgttaatgtt gcggtgcggg atcgttatgt gtttcctgtg ccacctcaat cgccggtttg
      841 ttctgcaata cggtccagat aaccagcgca cccagcaggt caaatactgc cagaactgcg
      901 aacagcgggc tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta
      961 cttgccagcc atgcggacat cccggttaaa ccgttcgccg tcgccacttc gttacgaccg
     1021 aacacgtcag aagagagcgt aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg
     1081 atacacagca gcataattgc gacatacggg ttggtgaaca gaccgatcat acccgggcca
     1141 atcatcagca cagcacccag cgttacgacc atcttacggg aaacgatcag gttcacacca
     1201 aaccaacgct ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac
     1261 agcatcggca tccaggcgaa catcgcgatc tctttcaggt taaagccgta aactttaaac
     1321 atgaacagcg ggatccacgc gttaaaagta ccccaggccg gttctgctag gaaacgcggc
     1381 agtgcgatac cccagaactg acggttacgc aggatctgac caacggacat tttcttcgcc
     1441 gtgctcacct ggtgctgggc ttcctgacca ttaataatat agtcgcgttc ttcatcggtc
     1501 agatgcttct ggtcgcgcgg atgtttatag aaaatcagcc atgccatcgc ccagataaag
     1561 ctcaatgcac cggagatgat aaatgccatc tgccagctgt gcatcacgat tgcccatacc
     1621 accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta gcctactgcg
     1681 atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc cgggatcatc
     1741 gctgcttccg cggcaccgac cgcaccacga gcaacagcca ggccacccca gctacctgcc
     1801 agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata accgattttc
     1861 gtacccagca catccagtac ataacctgct accggttgca tgaccgtata agcagcagaa
     1921 taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc catcagagtt
     1981 ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc gagcgtcacc
     2041 agtgcgatca tataccaacg taacccttta attttactca tgtaaaacct catcgttttg
     2101 ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt agttacgacc
     2161 gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc gtgctaattc
     2221 ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca atttgcattc
     2281 cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacctt aaaaggtgag
     2341 agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg aggccagtac
     2401 tggcgcggcc tgcagcgaga tttaccactt tgagagtaat ttttttaact acgtttattg
     2461 atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt tgtcagcatc
     2521 gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc cgtttatgac
     2581 tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga
     2641 ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg aagactatcg
     2701 ttttaaaaac ctgtatgaca tctggttgaa gggcgatcac tacaaatggc gcgctatgcg
     2761 taccaacggt gtggctgagc gtctgtgtac tggtgatgcg tctgaccgtg aaaaatttga
     2821 cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact ggacgcactt
     2881 cgaactgcgt cgtccgttcg gtatcactgg caaattgctt tctccgtcaa ctgccgatga
     2941 aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc gcggcatcat
     3001 gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt ctctggagca
     3061 tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga gctggcgtcc
     3121 ggacaaagcc ttcaatatcg aacaggcgac ctttaacgac tacatggcga agctgggcga
     3181 agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga ccaaacgtct
     3241 ggatcacttc gccgctcacg gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt
     3301 tgctgaagcg aacgaagcgg aactcgacag cattctggcg cgccgtctgg ctggcgaaac
     3361 cctgagcgag cacgaagtgg cacagttcaa aactgcggta ctggtgttcc ttggtgccga
     3421 atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta ataacaacct
     3481 gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg accgtccgat
     3541 ggcggaagag ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac
     3601 cattctctac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt
     3661 ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt ttaacgatca
     3721 gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtctgc tgagccgttt
     3781 tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg aatacttccg
     3841 ccgcattctg tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat
     3901 caacctgctg ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc
     3961 cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca tgcgctggat
     4021 aacgtcgcgg tcgctttagc agatttggct gaaggcacag aagtcagtgt cgataaccag
     4081 actgttacgc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac ggatatcgca
     4141 aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc ggatattgcg
     4201 gcgggggaac acgtccacgc ccacaatacg cgcacgaatc tgagcgatct ggatcagtat
     4261 cgctatcaac ctgattttca ggatctgcct gcgcaagcgg cagatcctga agtgcagatc
     4321 tatcgtcgcg ctaacggcga tgtcggggtg cgtaacgagc tgtggatcct gccaaccgtg
     4381 ggctgtgtca acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga gaccaacaac
     4441 gccgaaggta ccgacggcgt gttcctcttc agccacacct acggctgctc acaactgggc
     4501 gacgatcaca tcaacacccg caccatgctg caaaacatgg tgcgccaccc gaacgcgggc
     4561 gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg
     4621 ttgggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca ggatgatgag
     4681 atcgaagccg ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa cgataaacgc
     4741 gagccaggaa aactcagcga actgaagttt ggtctggagt gcggtggttc tgacggtctt
     4801 tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat tgctaacggc
     4861 ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt gctgatggac
     4921 cattgccgcg acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga cttcaaacag
     4981 tactttattg cccatgatca gccgatctac gaaaacccat caccggggaa caaagcgggc
     5041 ggtatcacca cgctggaaga caaatcactt ggctgtaccc agaaagcggg ttccagtgtc
     5101 gtggttgacg tgctgcgtta cggcgagcgt ctgaaaacgc cagggctgaa cttgttaagt
     5161 gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg ccatatggtg
     5221 ctgttcagta ctggtcgcgg cacgccatac ggtggatttg tgccgacggt gaaaatcgcc
     5281 accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc gggtcagctg
     5341 atccacggta aagcgatgcc gcagttgctg gaagaattta tcgacaccat cgttgagttt
     5401 gccaacggta agcaaacctg caacgagcgt aacgacttcc gcgaactggc gatcttcaaa
     5461 agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt
     5521 gaagcgccgt ttgcttttcc atacctgcta aggatttatc atgaccgcgt attggctggc
     5581 ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca atcgtgacga
     5641 acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac atttttttct
     5701 tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta cattgattac
     5761 cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg gcggaattgg
     5821 cctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggtacga ttgtcagtac
     5881 ctgggcgctg ttccgctgta aagggctcac catgcgctgc gtgatgtggt tttcaacgtg
     5941 ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacgatga ttgagggtag
     6001 ttttctgctt atgaatggcc tgaatatcat tcgtttctgg cggatgcaga aaaggggaat
     6061 tgatccgttt aaagtagaga aaacccctcc cgccatagac gaaaggggtt aaacaattaa
     6121 ttacgcaggg cgctatttgc cagacgatcg tcttccgcct ggcaagctgc cgcagtgaac
     6181 agcacgtcag ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac gccgataata
     6241 aagccgacgg caaccacctg catggcgata tcgttcgaaa taccgaacat attacaggcc
     6301 agtgggatca gcagcaaaga accccccgcc acgccggatg cgccacaggc acacagagaa
     6361 gccactacgc tcaacagcag cgctgtgggc agatcgaccg gaatacccag cgtattaacc
     6421 gcagccagcg tcaacacggt aatggtgatt gctgcgcccg ccatattgat ggtggctccc
     6481 agcggaatag aaactgaata ggtatcgcga tccagattca gcttttcaca cagcgccata
     6541 ttcaccggaa tgttcgctgc agagctgcgg gtgaagaagg catacacacc gctttcgcgc
     6601 aggcacagca gcaccagcgg gaacgggtta cgacgaattt tccaccacac cagcaatggg
     6661 ttaaccacca gcgccaccag taacatacag ccaaccagca cgaccagcag ttgcgcgtag
     6721 ccccacagtg tggagaaacc ggtggttgcc agggtagaag aaaccagccc aaaaataccg
     6781 atcggtgcga agcgaatgac cagtttcacc ataaaggtaa cggcattcga catatcattg
     6841 accaggtttt tggtggtctc gttaccgtga cgcagtgcga agccgaggcc gatcgcccac
     6901 accagaatcc cgatgtagtt acctttcagc agggcgtcga tggggttgga aaccatgctc
     6961 attaccagtc cgcgcatcac ttcgacaatg cctgacggcg gcgaaatatc acccgcgcta
     7021 ctggacaagt gcagggtaga agggaaggca aagctgaaga ctactgcggc cagagctgca
     7081 gagaaggtgc ccagcagata gaggaacaaa ataggacgga tattggtttt ctgcccgtgc
     7141 tggtggttag caatagatgc cattaccagc atcaacacca ggatgggggc aacagctttc
     7201 agtgcgccga cgaacaaagt acctaacaga ccaacagctt ccgccgcggg ttttgagatc
     7261 catgccagaa gaatccccag aacaaggccg accaggattt gttttaccag gctgccatga
     7321 gccagacgcc ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc ctttcattgt
     7381 gttgtcgcac atccctggtg cgttctgtcg acgcatcttt caggatgtaa caaagtgttt
     7441 gcaaggtcga gtataaggaa agttacagct tcgggaagcg aaaaatgctg gattttaaag
     7501 agaggtcatg ttttgcaact taatggttgc atttatttaa cataaacaga gggaaggact
     7561 ggctattaag gctgattaga taacatatgg gcaaaactat ttggaatatg agaacggttg
     7621 cgctccttta acgtgttatg ccatataaag tcactggcgc agggagcgcg cagggggcgg
     7681 ccaatcgccg ccgccccctg cccccccagg ctctggcgaa caaaatcgcc gctgcgcggt
     7741 tccctcagcc tatcccttgc ggctaccggg tcgggcgcaa ggtaacgtcc ctgtaagatg
     7801 cgccctcgcc ccggcctcca gggaacgctt cggcgatttt gacgccacca acacccgtgc
     7861 ggtctttttg taaagagcta taagccgatt acataattaa tagctcacct ttgtctggaa
     7921 tgagttatct ctaggaatgg cttaatatac agcgaatcat tctccccgct gcttatcatg
     7981 ccgataattc acccaggcgt tgataataaa cgtcatcacc agaatgccaa acaccacgcc
     8041 cagcgagacg gcgattggaa tatggtagaa gtcgacaatc agcatcttga taccgataaa
     8101 caccagaatc accgccaggc catatttgag catcgagaaa cgctctgcta cgcccgccag
     8161 caggaaatac atcgcacgca ggccgaggat tgcaaacagg tttgaggtca gcacaatgaa
     8221 cgggtcagtg gtcacggcga agatagccgg aatgctatcc acggcaaaaa tcacgtcgct
     8281 caattccacc agaatcagta ccagcatcag cggtgtggcg tacagcaatc cattcttacg
     8341 cacaaagaaa tgctcgttgt cgatggtgtc ggtcatgcgc aaatgaccgc gtagccagcg
     8401 caccagcggc ttgtcgccaa tgcctgattc atcttcatgg gcgagcgcca tcttcacgcc
     8461 ggtaaacagc aggaaggcac cgaagatata caggatccag tcgaactgcg aaatcagcca
     8521 gctgcctgtg aagatcatga tggtacgcag aacaatcgcc ccgagcacgc catacaccag
     8581 cacgcgacgt tgtaatgccg ccggaacgga gaaatagctg aacaacatca gccagacaaa
     8641 gacgttatcg accgccagcg atttctcaat cagataaccc gtgagaaagg ccagggcctg
     8701 tggatcggca acggcgcgac cttcggtttg caccagatac caccagaaag cggcgttaaa
     8761 cagtaacgac agcgtcaccc agaccagcga ccaggccgca gcctgtttca tggtcatggc
     8821 atgtgcccca cgacgcccct gcaacaacag gtcgatagcc agcataatgg cgacaacaac
     8881 agcaaatccg ccccatagca acggcgtgcc gacagtattc atagaagttc cttacacata
     8941 aaaaaacggc caacgtcgga agacgtcagc cgctgctttt tatgcataga cctcgccttc
     9001 cggcaaggtc tcacttacaa caaaaaagga atacgtctgc gtattccttt cgggttgccc
     9061 ggtgaccgga tgtggttttt cacacatcgt aatgacgatc gaccggcaat gaagttactc
     9121 ccctttgcag gtaacaaagt atgacagacc attcagtcag tcaatggtct gttacatacc
     9181 ttttacactg ttttactttg gcaagtatag ttttacgcta tctgccggaa aaatcacccc
     9241 agtctggcgg cggatctcgg tcagcagttt ggctgtgatg cgactgaccg ccagccctgg
     9301 atgatccacc agatgctcat ccaccagggt agcgaacagc tctgcttcgt agagcatggt
     9361 attaatatgc tgcggctggg tgagatcctg catctggctg ccacgcggca caaagcagac
     9421 tttctagcat tcagacagtt tttcaatcac cagcgatccc gcttcgccct gaatctcgct
     9481 tcccaggaca gaatcactga cctttgagtg ctgcaaggtg acgctgaaat caccgtaatc
     9541 catcaccacc acgccgtggg cgtcaacgcc gcttgccagc aaactcgccg ttgcctgcac
     9601 gcttttcggc tcaccaaata acgccaccgc cgacgccagg cagtaaaagc cgatatccat
     9661 aattgaaccg ttagagaatg ccggattaaa ggtgttggga ttctcaccgt ccaggtaacg
     9721 ttgataccgc gaggaatact ggcaatagtt gaaaaagact ttacgcagtt tgcctgcttt
     9781 cggcagcgcc tggcgcaaca aatgaaagtt cggcaggcag gcggttttaa atgcctcaaa
     9841 cagcaccacc tgattttcgc gcgcacaggc aatggcggca tccacttccg ccagattcga
     9901 cgccagcggt ttttcgcaaa tcacatgaat tttatggcta aggaaaagtt gtgtctggga
     9961 aaagtgcagg gaattcgggc tggcaatata caccgcgtca atggcatcgc tttccgccat
    10021 cgcttccagc gacgtaaaca gatgctcgac agaaaaatca ttggcgaagt gctgagcctg
    10081 ctcaaggctg cgggaatata cggcggttaa cttgtattta ccgctctcat gggcggcctc
    10141 gacgaactgg cgagtgatcc agttcgtacc aatcacagcg aaacgtatca taacgcgtgg
    10201 atactccata aagggattct gtcgtcaatg tagcacgcct ttttcatctt cgacgtgcgc
    10261 agatacgcat tattctttcg ccggacagcc atgctcacta aacggcccaa tcatgaccga
    10321 tggcccgaga tatttcggct ggcgatggag gatttttgcg tcaatcaccg cgctgacacg
    10381 agcgagtttc tcccgcagtt gagtataaaa accatgcttc atattgaggt ctggcgcgtt
    10441 aaacccgata gcgtcgatgc cgtactgttt tgccagccag atggcgcgtt ggttatgaaa
    10501 ttcttgcgag ataatggtga tatggttttc cccaaacacc tttttggcac gcactaccga
    10561 atccagcgtt gagaatccgg catagtcgca aaagatcact ttcgccggta cgcctttagc
    10621 gatcaaagcc tgctgcatac ctgatgcttc atcgtaattt ttacgtccgt tatcgccact
    10681 caccagtagc catttcactt ttccggcgtg gtacaacgct gctgcggtat caatacgtcg
    10741 ggtaaagtag cgattacccg gcctcgcccc cagcaataaa cccacgttac gcgccgggac
    10801 ggcgttgaca tcgccccagg tcagctgctt gctggcgttg accataacgc gatcggcgat
    10861 aaaaatggtc gctcctgcca caagtaacag taagcaggca atctttagag tccggcgaga
    10921 aaaacaaata cggagaagaa ggcgggcaaa tgcgcgcagc atggtcgtta tccttaacga
    10981 aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga catcattttc
    11041 agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg aactcaagct
    11101 ggtggtactg cggctccata tggcagcaca attgatagaa cgctttgtta tgctctttct
    11161 ccttcaggtg cgccagctcg tgcaccacga tcatgcgcaa aaacggttcc ggcgcgttgc
    11221 gaaacacggt agcgacgcgg atctctgctt ttgctttcag cttgccgccc tgcatacgag
    11281 aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg tcatacatca
    11341 ctttattgat cggcggggca ttacgcagaa actgattttt cagatcctga gtatattgcc
    11401 agagagcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc
    11461 ccagacgctg ttcgttaatc agtgttcgca cctgcgaaag cagctgctcg ggataaccct
    11521 gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt atactcacgc
    11581 acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga gccacttaga
    11641 caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg ttaacccgct
    11701 acaggcgtgg gaagcggcag atgaatattt gctgcaacag ttggacgaca cagaaatccc
    11761 cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca
    11821 taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg agaatttacg
    11881 cctaaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg actacccgca
    11941 acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg aacagcaact
    12001 gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg ccaaagcccg
    12061 tgacattcac acttccacgc tggaactgtt cgaaaaagtg ctcggcccga ccaccaccac
    12121 gctggcatgg aaaaaagcgc gcctgattaa ttgcacgttc aatgaaccgc cgctggccga
    12181 tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc ataaccatgc
    12241 gaatgtcttc tcccgcaccg ggcttgatat cggcgcgcgc ttctttatgc aacatctgcc
    12301 agagaatctc gaaggggaga ttgtcgatct cggttgcggt aatggcgtta ttggtctgac
    12361 gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt
    12421 tgcttccagc cgtttgaacg ttgaaaccaa catgccagag gcgttggatc gcagcgagtt
    12481 tatgatcaac aacgcgctct ccggcgtgga gcctttccgc tttaatgctg tgctctgcaa
    12541 ccctccgttt caccaacagc atgcgctgac cgataacgtc gcctgggaga tgttccacca
    12601 cgcccgccgc tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc gccatctgga
    12661 ttacttccat aaactgaaga aaattttcgg caactgcacc actattgcga cgaataataa
    12721 atttgtggtg ctgaaagcgg tgaagctggg acgtcgtcgg taagattggg agttacctgc
    12781 cggatgcggc gtaaacgcct tatccggcct acgttcagca cgttaccccc aggccggata
    12841 agacgcggca agcgtcgcat ccggcaatgt ctgctcagtt ctggaatcgc gcttccataa
    12901 aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttttt ctacgtttat
    12961 tcttccgtca cacagataaa ttcatccgtt gccaatctgt caacggatgt tatcatattg
    13021 ccaatttgtc aacggacgtg atgaagaatg cacctgataa ctcaaaaagc attgaaagat
    13081 gctgcggaaa aatacccgca acataaaacg gagttggtgg ctctggggaa cacgattgct
    13141 aagggatatt tcaaaaaacc tgagtcatta aaagcagtat tcccatctct ggataacttc
    13201 aaatatctgg ataagcatta tgttttcaat gttgggggca atgaattacg tgttgtagca
    13261 atggtctttt ttgaatcgca aaagtgctac atacgtgaag ttatgacgca taaagaatac
    13321 gatttcttta ccgctgttca tcgtactaag gggaaaaaat gattgcgatt gccgacatct
    13381 tgcaagcagg agaaaagcta actgctgtgg caccttttct ggcgggtatt cagaacgagg
    13441 aacaatacac ccaggcgctg gaactggtag atcatctgct gctcaacgat cctgaaaacc
    13501 ccttgctgga tctggtgtgt gccaaaataa ccgcgtggga agaatcagcg cccgaatttg
    13561 cggaatttaa tgccatggct caagccatgc ctggcggtat cgccgtgatt cgtaccctta
    13621 tggatcaata tggtttaacc ctttccgatc tgccggaaat tggcagtaaa tctatggtgt
    13681 cacgcgtttt gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa ttggcaacgc
    13741 gattcggcat ttctcccgcc ttgtttattg attaacgcga cgtgccggat gcgttgcatc
    13801 cggcgcttca aataacgatt taaatctcca gcgccagccg tgttccctgg gcaattgccc
    13861 gtcgtgcgtc cagctccata gccacatcgc agccgccaat taaatgcaca gttttcccgc
    13921 tatcaatcag cggttgcgcc agcgcgcggt ttggctcttg ccctgcgcag atcaccacat
    13981 tgtccactgc taatacctgc gtttcgccgt tgatcaccac atgcagcccg tcatcgtcaa
    14041 tcttctgata acttacgcct gggatcattt tcacaccccg cgagagcagg gtggtgcgat
    14101 ggatccagcc ggtggttttg cctaacccct gtcccggttt gctggctttg cgctggagca
    14161 tcacaatttg ccgtgggcta cgcgggatct gcattccctg cgggcttaag ccaccagcct
    14221 gttgtaggct gctgtcgatc ccccactcat tacagaacct ggcgatattc tggctggtgg
    14281 attcgcccgg ctgacttaaa tacatcgccg tatcaaaacc aatcccgcca caaccgatga
    14341 tggcaacctt gttgcctacc ggcgctttgt cgcgcagtac atcgagataa ctcaatacct
    14401 tcggatgatc gatcccgtcg ataggcggaa cacgcggcac gatcccactg gcgaggatcg
    14461 tttcatcgaa cgcctgtaac tgatccgccg tcacggtgtg attgagtttc agcgtcacgc
    14521 ccgtcacttc gatcatccgg cggtagtagc gcagcgtttc gtaaaactcc tctttgccgg
    14581 ggatctgttt ggcgatatta aactgcccgc cgatctcgct gtgcgcatca aacaatgtta
    14641 cctgatgccc acgcgccgcc gcgttaatgg caaacgccag cccagcaggt ccagcgccga
    14701 ccaccgccag attttttttc tgcacggcgg gaaggattgg cattttggtt tcgtggcagg
    14761 cgcgaggatt caccaggcac gaggtgactt tgccaacgaa gatctgatcg aggcaggcct
    14821 ggttgcagcc aatacaagtg ttgatctcat cggctcgccc tgattgcgct tttgacagca
    14881 gttccgcatc cgcgagaaac ggtcgcgcca tcgatactat atcggcatcg ccgcgcgaga
    14941 gaatatcgtc ggcaacctgc ggatcgttaa tccggttggt ggttaccagc ggcagcgaga
    15001 cgtggccttt cagtttgcgc gtgacccagc taaatgcgcc gcgcggcacg ggcgtggcga
    15061 tggtcggaat ccgcgcttcg tgccagccta ttccggtgtt gataatggtc gcgcccgccg
    15121 cttcaatggc ctgcgccagt tctaccgttt cggcaaaagt gccgccgcct tcgaccaggt
    15181 cgagcatcga caaccggtaa ataataatga agtcgttgcc gacgcgttcg cgcaccgtac
    15241 gcactacttc tacggcaaat cgcatccggt tgcggtaatc gccgccccac tggtcactac
    15301 gctgattggt gcgcagcgtc agaaattcgt tgatcaaata cccttcagag cccatcactt
    15361 cgacgccatc gtatccggcc tcccgcgcca gttgcgcgca gcgggcgaaa tcgtcgatca
    15421 gttgcaggat ctcttcatgg gtgagttcgt gaggaacgaa acggttgatg ggggcctgca
    15481 atgcggacgg tgcgaccaga tgcggttggt agctgtagcg cccggtatgc aaaatttgca
    15541 gggcgatttt gccgccttcc tgatgtacgg cttcggtaat ggtgcgatgg tgtgggatct
    15601 ggctggcgtc gttgagcatt gcgccacctt ccatgccaac gcctattaaa tctggtgcaa
    15661 tgccgccgct gacaatcagc gccacgccgt gacgggcgcg ttcggcataa aacgcagcca
    15721 gccgttcagc accgtccggg tattcctcca gcccggtgtg cattgaaccc atcaacacgc
    15781 ggttttttaa cgtggtaaaa cctaaatcca gcggggcgaa cagcgacggg tagctcataa
    15841 aattgtccag tatgtaaaat aattgttacg gatgagttct aatttagccg tcgatggaga
    15901 aaagggaaaa ggggagtgcg gggtttgtga tgggattcaa aaagaatacg cccggtggta
    15961 acgccggatg cgacgcttgc cgtgttttat ccggcttggg ggattgcaca tgtagggcgg
    16021 ataaggcgtt cacgccgcat ccgccagccg ttgcaaatta ctgcttacgg aaaaaatcgt
    16081 tatacaacat atacaaatga gcggcactcc aggagaaatt tggtgcgcct tgctgcgcgc
    16141 ctgtcagcgg gttgtaattc tcctgaattg ggccatcggc ggttaatcct ttggcgtgcc
    16201 tgaagaacgt atccgccagc ttcagggcat catcacgata accgtaacgc tccatccctt
    16261 tcagaccaaa ccagaactga tccacccata cgcgcccgcg ccagtagata tcagcgccaa
    16321 aagccggatt ggttaacgcc gccgttccca gcggaacaaa ggtgttgaac tctttaggat
    16381 cgagcatcac cttcaccacc gcgtcggcat tggcctgcgt tgccgcaccg ttaaacagcg
    16441 gcgaccagcc ttccggccct ttaccgcgct caacaatcgg tttgcccgcg cagccgttcg
    16501 ccagtggttt atcttcaata cgtacgtcat agtagaactg ggtagtcggg tcgaacatac
    16561 aggtgttgat gtagtccgcg agctgctgtg ccaactggcg atagcgtttg gcctcttctg
    16621 gcttaccgag gatggttgcc atctccgcca gataatggtt atcgctgtac atatagctgg
    16681 cctgatccac cgactcctgc aatagcgagt agcccagcaa cgttccgtcc tgactgcggt
    16741 tttcggcgaa tttcaccgtc tagtcgctac gtttgccgcc attagcgaca tatttatcca
    16801 actgttcttt gtcgataaaa ccaaagacgg cggcatcatc acgccccgat tcccacgacg
    16861 cagcgacctg tgccggaatt tccagactgt cgtactggcc tttctccacc acgcgggcgt
    16921 agttgttcag cccagactgc gtctcttctt tatcgccttt tttcaccgta aacagcatct
    16981 cgccgctctc ggtgttgtgg gctttgtcgc gggtcgcgcc atattccggc accccgttgc
    17041 cgttatgatc gcggttacgt aaccaccagt cgtgataggc caccagtttc gggtacatct
    17101 ctgccaacca ggctttatcc tgggtgacgt tgtatacttc catcaccgac caggcggcaa
    17161 ggctgggttt ggtattacgt tcgttccagt tgccgccatc gccaccatgc tcggggctaa
    17221 ggttccacgc tatcaggtcg gggacaaagc ccacatcctg cggacgcacg ctgtcgccag
    17281 gctggatctg ccaggagaag accgcgcgga tattctcttt ggcgatatcc ggattgaaat
    17341 gcgccatcgc aaacgcctgc ttccaggtat cccacggcca ggtctgattg ccggagaacc
    17401 agcgtccggt caccgacggc gtgacggtgt tataattcac cgcaccgccc ggtgagcgcc
    17461 agttaccgtt gagcgtttcg atcgctttca ccgcgacgcg cgtctgttcc ggcgtcgcat
    17521 ccggattggt taaccctttc ttcaggtatt cttcccagcg ttgctgcgag gcggtgagat
    17581 aaaacgccgg acgcgccaga atatcgcgga tctgcatttg ctctttgcta acttcctgag
    17641 cggtcagcag atgggaatag gtggtgtaga gcgtggtcga accgttgata tgcgccttac
    17701 tggtaaagcg attaccgttg atttcagtct gcaccggcag ggatttatgc acctgatatt
    17761 ccgattcgcc ggaggtcagc agatcccagg tggcgcgcac tttgccaaag gtgactttca
    17821 ggccatcacg ggtggcgctg attttgcgct gatagtcagg gtattcgcca gcaatggttt
    17881 tatcggaaag cggtttccct tctttcgctt ccagtttttc cagcagttcg ccatcccaca
    17941 ccagatccag cggtttattg ctgatgattt tagtttccag tagtgacgtg cgcggcgtgg
    18001 cgaagcgcag agtcatttcg acctgcacat cttttgctgt cagtttttgc accagcgcgc
    18061 cgggaatact gtatgcctcc agcgtgaagt tgactttctt gccgtcttgc cagacagtca
    18121 ggcggtcgaa attgctggcc ataaagttga tgtattcttc cgtcagcagc gcaacgcccg
    18181 gaaagccgcc catggtgttc gggccgtctg gcaacagatg accatgccag gctccgagat
    18241 cgaaaaacgg attaaaacgc tggtgatcgt cgtaatcgta atccttcatg tactgcggcg
    18301 caccagtacg gttaatcacg tttttataat tgtcggcgtt agcggcatga gcggaaaaac
    18361 ttatcagcag agcgcaggtt actggcgtta aaatagtttt gattttcatg aaattacagt
    18421 ccttattacc agtagaaata ttgcatcagg aagatttggt cgctgggatc gttagaggca
    18481 tttttcatgt agaagcggct atcaaaagcc gtggcgaaac gtccgccgcc atattcgtac
    18541 cagataccaa actgcatgaa agaggtggtt tcatcgtcag cgtggtcagg gcgatgtttg
    18601 gcgtaggtgt aagcggtgga gagataaacg ccctttaacg cttcatacat ggcgctcacc
    18661 atcatcccgc tttcacttcc cggtacgtcc agcccgtcgg cacgtccggt atggtgccag
    18721 gcgcgagcgg caaagttagg ggtaaacagg cccgtgagat agagttgtcc tgtgccgtcg
    18781 gtaatttcca gaccattcat ccaggtgatg ccttcggcaa ggtcatactg gatgtaaccg
    18841 ttcatccatt gccggatagg tgtatttgtc gtcgtagcga tcgtacttgc caaaatgtag
    18901 ccaggctttg ctttcgtcat gatgtccggc aggtgtggcg gtgacgctat aacgaaagtt
    18961 gccgcttaag ttctgtacct tcagcgcggt cataatgtcg cgggtattgg gaatgacgta
    19021 gcccaaatcg ggtgtgaagt cgccccacca ttgcaggtca tccagcgagg aatcctgacg
    19081 cagactgagc atgatttcgg tgccatcatt ggtacgataa ccgccataga aacggttaat
    19141 cccaccttcg aaaccacccc agctatggtc aggcacctag gcattaccgt cgtggtcggc
    19201 ctgcacggtc cagccttcgc cgtaaatcag gccgaaccag gagccgtact tcatctccag
    19261 gccaccttca atataggtgc cgtcgctgta tttatgttta ttctcaccct ctaaatccat
    19321 atatccacca agccctaatt cgccgtaaaa acgaaatgaa ggagaagatg tcgaggtaat
    19381 tgcgggtgtt tttgtgtcgt catgttctgc cggacgtgct tcttctcctg ccgcagaaaa
    19441 ggcgtagcaa aacggcagag caagtaatgc tctgcatggt gcagtgatta atttcattgt
    19501 gttaatccat gagctaaaga aaaagtacag ccttttatcg ttgtaattat aaaaatataa
    19561 aggcagaaac tattttccct ctcctcatcc aggaagaggg aaaatgcctg ctcatttttt
    19621 attagtctgc ttaatcaaca ttgctggctg gcgtcgcttc aatatggcgc tcttcagccg
    19681 ttaatccctt tatatattta ctgtatttcc accacgcaaa gccgaggaag ataacaatac
    19741 cgccgacgtt ataaaaaatg atggtcagaa tattcgcgcc agtcgggaat gtcgaagcga
    19801 caaaccctac ggcaaatatc gcaatcagca ttgaaacaac gataataccg gtgcggcggg
    19861 agcccatacg gaaatcgcgt ggcaggtgat ctaatttggc gcgtaaattc aggtaagcca
    19921 gcatgatgaa taacggcgga agcatggacg ctgcggcggt catattaata atggtattca
    19981 tcagatcctg cacggtattg gaaccgagca tcgggataat catcagcggg atgacgatca
    20041 gaaactggat ccatgctgcg cgcgccggaa cgccgttttc gttaagttcg acggttttct
    20101 taccaaagat gccttccggg atttcggaga agaaaatttt caccggcgtt gcggtccaca
    20161 tcagcaggga accgaacatt gcggtaaagg acaccagacc gacaaagcga ttcatcaacg
    20221 cttccggcag accaaaatac gccgccatgc cgtggaatac ctgcaccgat ccgccggtaa
    20281 atttcaactc tttgctgctg acgaagacgt taatcagcac cgaggagacg gaatacagta
    20341 cgccgataaa aatcccggcg aggatgatca ctttaacgaa cgatttcgaa ccgcctttga
    20401 cgtcgttaac gtacaccgcg acggactccg cgccacctgc ggccataaag atccaggtgg
    20461 taacgccgag gaacgcccag ttgaagttcg ggatcatcgc gtcaacggtg atcggatcgg
    20521 caggctgtac gtcgccaacc agtgccgtac ctgccagtaa aatgtaggag agcgttaaca
    20581 gcagcatcag cgttgaagtg acggaggtaa ttggccccag cattttcgcc ccgttggtgg
    20641 aaacccaggt ggagaaggcg aacagcacca tactgataac ggtggtggca accggcgtca
    20701 taatatattc gtagccgagg aaggcatacg aagcataagc aataacgcgc ggcaacagcg
    20761 aggtgaaaaa gaacagattt acgaaccagt aagtgtaggc agtaataaat gcccaacgtc
    20821 cgcccagcga actttttacc cacgcgtaga caccggcttc tgagttttta tttaacgaaa
    20881 caaattctgc gatgatcaga cagaagggaa taaaataaaa aatcgtcgcg aggaaaaaca
    20941 tcggtgccga ggccagtcca agctcaatat tattattgat aacgttgtta aagctgtaaa
    21001 cggcggcaaa agtcagcgag agcaagccga atttgccgat tgtattacgt ttggtatcag
    21061 acataacaca tctccgaaaa tgtgtagggt agcggtggcg gcaaactcct tttgccgccg
    21121 tagttgttat ttgttatgga aataaccatc ttcgatggtg actttgagaa ccactttttt
    21181 gaccgcgtta ttgcagataa accgatacgc ttcatggata tcgcaaataa cgatttgccc
    21241 ttcgtggacc tctacggttt ctccgcagcc ttttaaatat tcacggtcag tttcatcacg
    21301 ataatattcc actacctgta atgtctcttt cggcgcatat tcaatttttt gctgcccttg
    21361 caggtagtaa tgcacttcaa aatagcgtcg atgcccggta aatagcgcat gggtcgcgga
    21421 gtcattttcc acacggtagg tcaatgaatc accgatggag tgggcgacgc caggctgaat
    21481 gttgtcgata ttttcaatcg cttcaacgca gcgttgccac ttcttgccag aggcgtaaat
    21541 ctggcggaac tgttctaagt tatcgatgat cctcattgct tattctcgct gtgcaaattc
    21601 gtggaaaaga accctgcgcc gaactcatac gacgccaggc tttgcgcggt agcttctccg
    21661 ccagaaaccg gcagcaacgt aaagccgtag ctgaagtcac ggaaccagac gcgccaggag
    21721 tccagcacct cgctgcccca ggagttggag ccgaggccaa gtagctggtg gtcgaggttc
    21781 agggtgatgt catcactgcg ctgcagctcg ttacagtgct gggcagcgtg gatgttttcc
    21841 tgggtatagt gccaggcgct gaagttaatt gggcgctgcg gaaccaccag cagaccgttg
    21901 tcgtggcggt tagtcagtgc cgtccagcgg acatgctgac ggttgccgtt gttctgcggg
    21961 aagggatagt tctcgaacat ggcatcgacg gtgctgcgcc agatatcgat gatgttagcc
    22021 tgctggctgt cggcgtagtt ttcgcccggt ccacgaccgt aatacgccac ctgatcgtat
    22081 tcgccgttaa tccccatggt gaaaccgatg cacggaatga tgtgcggata gtcgccgtaa
    22141 cgctcgccgg aaagcgccac gttaacctgg ccatcggcag cgatgcgcca gatgtaggta
    22201 cagcgcatcc cgaagtcaaa caccggcggt gcaataactg tgcggctgat gatcaacact
    22261 tcaccatcgc tctgttctac ggcaaagtcg cgcagatgtt cctgcatgat ctgcaaatga
    22321 ttcggctgcc acaacccttc gtactcctgc ttgtggttgt cgatcatcgg cttgaagaag
    22381 ttgatctttg gctcgcgggt cagcagcgac tcgccattta cctgccagga tgtcggtttg
    22441 ccactcattt ttgagaaggt gatcgcgaag ttgtagccgc gaacggtgca gctcaaacga
    22501 tcgtcttcca gcgtcagcgg acgcgcatta tttggtgcga aaggcactgg ctgcgcggtg
    22561 ttttccttca gcgggaactg ataagtggcg atagaatgcc cggcttcgct gtagcgggtg
    22621 cgggaatctt tggtcaccgt aatgttgagg aacgcttcgc gggcgtccag ctgcggcagc
    22681 gtgatctgca agggggcttc gctgttcggc gcaacgtcgc gcagtttaat ctgctgcgtc
    22741 gcgagcgttt caccttcggc gcgcacctct gcgtgcaggg tgtagtcatc aagcgtggta
    22801 aaccacagtt tattttcgac tttcaactcg ccgcgagtca gcgcgtggat ttttaccggc
    22861 gcgataacct gtttgtactc tttcaggccc gatcccggcg tctgatcgga atagatcaaa
    22921 ccatcaagac agaagttata gttgttggga tagtcgccgt agtcgccgcc gaatttatac
    22981 cagacattgc cgtggtcgtc ctgtgcctgg atcccgtggt cgcaccactc ccagacataa
    23041 tgaccctgaa tgcaatcgtg cttatagaag acgttctggt actccgtcag cccgcccggt
    23101 ccgttcccca tcgcatgagc atattcacag atgatgcgcg gcttcggatg cgggtattca
    23161 ccaaactcat tcatcagcgg cacgcgggtg tacatggtgg aaataatatc gaccacttca
    23221 gcatcgcgat cttcttcgta atgcaccagt cgcgtgtcat ccagcgcttt cgccgcatgg
    23281 tacatcgcgc ggatgttaca gccatagccg gattcattgc ccagcgacca gatgatgatc
    23341 gacggatggt ttttctgtgc gtggatatgg cgaacaatgc gctcgacgta gactttttcc
    23401 cactgcggat cgtcggtaat acggctgata tcgccgacat tagcaaagcc gtgcgattcg
    23461 acgtcggttt ccgccatcac aaacaggccg tagatatcac acaattcgta aaaacgcgga
    23521 tcgttcgggt agtgagcggt acgcacggag ttgatattgt gctgcttcat caactggaga
    23581 tctttctcga cgcgatccat tccaacggcg cggcctttgc gatgatcgtt gtcgtgacgg
    23641 ttgacgccgt gcagcatcac ataacggtta ttgatccaga acagaccgtc gcgcactttg
    23701 atatcacgga agccaacgcg ttgtggcacc acttccagaa cgttgccgtt ggcgtctttc
    23761 agcgtcatga ccagatggta aagataaggg gattctgctg accattgctg cggctgttcg
    23821 acagtaaaag caaagctggc gctggtcagt ttttcaattg ccaaatgatc aatggcgctg
    23881 ctgtgcacca cgcgttcgcc atcaaacagg gtatattcca gcgtcgtgac gacaggggag
    23941 gcggcgagat tttccagcac cacttcgcag gaaagcgtgg catcgcaata ggcttcgtca
    24001 aagtcggtac gcacagtgaa atcgttaata tgcgttaggt gttttccgac cagataaaca
    24061 tcgcggaaga tccccgccga ccaccacata tcctggtctt ccacgtaggt agagtccgcc
    24121 cactgcatca cgcgcacaca caacaggttg tcgccggttt taaccatcgc gctgatgtca
    24181 aactctgcgg tcaggcgact gcccttgctg aaacccacat actgaccgtt aacgtagact
    24241 tcaaaatagg tttcgacgcc gtcaaattta atcagcgtct gtttaccctg ccagccgtcg
    24301 ctgagggtga aaatacgttg ataggcaccg gttgggttat cgctggggac aaacggcaca
    24361 tcgatgggga acggaaaacc ttcgtcggta tattgcagtt tgccgtgacc ttccatttgc
    24421 cacatggcgg ggacggtaat atgcccccag tcagccatta actcagaggt gaaggcttct
    24481 ggtacttgca gcggatggtc aaaaaagtgg aaattccact gaccgcttaa gggcagaaac
    24541 aggctgctgg tttcgcgggc aaaggtacgc gcttgcgcaa cagaatcata tgaaaaaaag
    24601 tacgcacgcg gcgcaagtcg gttttcgtgg gtgagctgaa tgttttccca gcgattcata
    24661 ggacctcccg gtagagagaa atcggaacca ttcatggtga tggtagtttg tgtaattgct
    24721 gggggggtta gtaaaatttt tactaaaagt agcaggtaaa tgcatttact ttaactggat
    24781 cacgaaaata aacggcagca ctgtccggca aaaatgattg ccggacagtg tgtaagggga
    24841 tttaacgggt cgtgccgcgc agttttaatt tgctgggaac gaagactagc agcggcagcg
    24901 cgcgaccatc gcgggctttt tcatacacca ggttaacgcc ctgacttccc atcatctcgg
    24961 aatggatgcg cacggtggag agcggcggaa aggtaaatcg cgcggtgggg atatcgttaa
    25021 cgctgataag cgaaatatct tgtgggatgt tcaggccacg ttcatgaatt gcccgcagta
    25081 cgccgatagc aatggaatcg gaagcaacaa acagtgcctt cggatagtct tcccgcgcca
    25141 gcatttgttt tgccagttca taacccgacg aactggaaaa accgccgcgc cagatatcct
    25201 cttcgcgtac cacttgtttc agtcggccat attccgcaaa ggcgacctca cgaatatccg
    25261 ccttgccagg ctcatcttca ccgccaataa aaccaatacg attaacgccc tggttgatat
    25321 agaagtcgat gatttcttta ctgatgcgtg ccagatcgat atccaccgca tcgtaaccgc
    25381 tgccgggttc gtgaaagtcg ataaaacaga tattgtcggt caacgcgctg gcagcggcgc
    25441 gcagggcggg cgtgggtttg ccgacaatta aaataccggt gacgttttta atgtctggta
    25501 atccgttgtg ttcataacag ttggtcagtt cgatcgccag cttttcgcac tgagtttcaa
    25561 tgccgtggcg gatcgccaga tagtaaggat cgttgatctc cagctcctgc tggtagctgt
    25621 agatagccag aatatggtgt tggttgactg cgcctgtctg tagtttacgg gcgctactgg
    25681 tcttgtactc cagcttttcg gcgatctcga gaatgcgatg tttcgtctct tctttcacat
    25741 tcaatgtcgg atcgtcattt aagaccctgg atactgtcgc cagggatacg ccagcttcga
    25801 ttgcgatgtc ttttaatgtt gccatgttta ccttttttac tttccctgat gtgcgccccc
    25861 attgtagagg aagtgcttat cgggtcacct gttttcagta aataaagcgc gtttctttga
    25921 cgctgtaacg gtaacacagc ggcttaggaa tgtgatcacg cctgcattct ccatttttag
    25981 cctacaggat tgagaaaaac ttttgctata gtttagtaaa attttacccg aagggaggaa
    26041 gcaatggaaa ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt cggaaagatt
    26101 gtggaagtga aacgccatga aaacgccgac aagctgtaca tcgtacaggt tgatgtgggg
    26161 aaaaaacact gcaaaccgtg accagcttgg tgccgtacta cagcgaagag gagctgatgg
    26221 ggaaaacggt ggtggtctta tgcaatctgc aaaaagcgaa aatgcgtggt gaaacgtcgg
    26281 aatgcatgct gttgtgtgcg gaaaccgatg acggcagtga aagcgtgttg ttaacgccgg
    26341 agcggatgat gcctgcgggc gtacgcgtcg tataaaaaga tcggatggcg acgtcgtatc
    26401 gccatccgat ttgatattac gcttcttcga cacttactcg catggccgcc agcgccttac
    26461 gcgccgcttt gatcaccagt tcacactgtt caatggtcag tgtcagtggc ggttcaatgc
    26521 ggatcgtttt ggcgttattg agcgttccgg ccaccagtac gcgctggcgg aacatctcgc
    26581 tggcaaagtt atagccgatt tcgttatcaa caaactcaat cgccatcaac atccctttac
    26641 cacgcgcttc ctgtaccaga tcgggatatt cccgcgccag ttgacggaaa ccgtccagca
    26701 acatatcgcc tttttgctca gcctgagccg gtaagttctg ctccagcaac acattgatgg
    26761 tcgccagcgc cgccgcacag gccagcgggt tgccgccaaa ggtggtggta tgcaggaatg
    26821 ggttgtcgaa cagaactgaa aacacctctt cagtggcgat ggtcgcgcca atcggcatca
    26881 cgccgccgcc gagggctttt gccagacaca gaatgtccgg ctgaacgttc tcatgttcgc
    26941 aggcaaacat cttgcccgtg cgccccatgc ccgtttgtac ttcatcgagg atcatcagtg
    27001 cgccgaactc atcgcacagc ttacgcacgg cggtgagata gcccggcggc ggcagaatta
    27061 cgccaccttc gccctgaatc ggttcgagga tcaccgcagc cacatcatca ccggtttttt
    27121 tgcactcatt aagagccgtg cgcatggctt cgatattgcc aaacggcaca tgacggaacc
    27181 ctggcagcag tggcataaac ggtttgcgga aggtcgattt cgccgtggcc gacagcgcgc
    27241 caagtgattt accgtggaac gcgccgctgg tggcaataaa agtaaatttg ccgcgcggcg
    27301 actgataagc cttcgccagc ttcagcgccg cttcaacgga ctcggtgccg ctattacaga
    27361 agaagctgta tttcagttta ccgggcgtta gcgcagcaag ggttttcgcc agcatagccc
    27421 gtagcggatc gagtagctcc tggctgtgca gtggttgttt cgcaagttga ttctgtacgg
    27481 cggaaaccac aactggatta cggtgcccca cgttgaaaat tccaaaacct cccaggcagt
    27541 cgataaactc ctgtccttgg gtgtcgacaa gcgtatttaa acctcccgct tgccactcta
    27601 cggctccgta atccccgccg gcggtaacag atttgcgata ctctaaaaac cccggattga
    27661 catgctcttt gaagtattca atcacctctc ggttaagtgc tttcatctcc tcatgatcca
    27721 gcgttcgctt ctcaatgaga ttcagggcgt gggcgctgca cgctaaagcc gatgcgctcg
    27781 aaggtaacct gttcaaaata tgctccggag gctcgcgtat cacatgatac cgatttaagt
    27841 attgcaggga ttgcgccact ccggctgcga tcgcgaaaat cgggataaac acaaatgcgg
    27901 ttatcatttg tgcaatattt aatgctatcc gttaacattt gttgttattt tgcacggttt
    27961 tgtcgggtat ggatttgatg attgctgcgc aaatgcacgt tttcagggca ataattgcct
    28021 gatttttggg caagataatt aacgttttcg tatagtaaca gagtgaaagt attttaaatc
    28081 aaagaattag atttgtgctt taatcgcaaa ttgcgatcta aatcaaatta atcggttaaa
    28141 gataaccgca gcggggccga cataaactct gacaagaagt taacaaccat ataacctgca
    28201 caggacgcga acatgtcttc tcatccgtat gtcacccagc aaaatacgcc gctggcggac
    28261 gataccactc tgatgtccac taccgatctg caaagctata tcactcatgc taatgacact
    28321 tttgtgcagg tgagcggctt taccttgcaa gagttacaag ggcagccgca caacatggtg
    28381 cgtcacccgg atatgccaaa agcggcgttt gcggatatgt ggttcaccct gaaaaaaggg
    28441 gagccctgga gcggcatcgt gaaaaatcgc cgcaaaaatg gcgaccatta ttgggtgcgg
    28501 gccaatgcgg taccgatggt gcgcgaggga aaaatcagtg gctatatgtc gattcgtacc
    28561 cgggcgacgg atgaagagat tgcggcggtg gagccgctgt acaaagcgtt gaactccgga
    28621 cgtaccagta agcgtattca taaaggcctg gtggtgcgta aaggctggct gggtaaactg
    28681 ccttcattac cgcttcgctg gcggacgcgt ggagtgatga ccctgatgtt tatcttgctg
    28741 gcggccatgc tttggtttgt tgctgccccg gtggtgacgt atttcctctg tgtgttagtg
    28801 gtattgttgg caagcgcttg ttttgaatgg cagattgtgc gcccgataga aaatgtcgcc
    28861 cgtcaggcac tgaaggtggc gaccggagag cgtaatagtg ttgagcacct gaatcgcagc
    28921 gatgagctgg ggctgacatt acgcgcggta gggcagcttg gcctgatgtg ccgttggcta
    28981 attaacgatg tctcaagcca ggtgtccagt gtcagaaatg gcagtgagac gctggcgaaa
    29041 ggcaccgatg aactgaacga acatacccag cagacagttg ataacgttca gcaaacggtg
    29101 gcgaccatga accaaatggc ggcgtcggtg aaacagaact ctgccacggc gtcggctgcc
    29161 gataaacttt ctatcaccgc cagtaatgcg gcagtgcagg gtggggaggc gatgaccacg
    29221 gtgatcaaga caatggacga tatcgccgac agtacccagc gcattggcac cattacttcg
    29281 ctgattaacg atattgcgtt tcagaccaat attctggccc tgaatgcggc ggtggaagcg
    29341 gcgcgtgccg gcgaacaggg caaaggtttt gcagtggtgg caggggaagt gcgtcattta
    29401 gccagccgca gcgctaatgc tgccaacgat attcgcaagc tgattgatgc cagtgctgat
    29461 aaggtgcaat ccggttcgca gcaggtacac gccgccggac ggacgatgga agatattgtg
    29521 gcacaggtga aaaacgtcac ccagttgatc gcccagatta gccattcaac gctggaacag
    29581 gccgatgggc tttccagcct gacccgtgca gtggatgagc ttaacctgat cacccagaaa
    29641 aatgccgagc tggtggaaga gagtgcgcag gtgtcggcga tggtgaaaca ccgcgccagc
    29701 cgactggaag acgcggtgac ggtgctgcat taatcggtat gccggatccg gcggttggag
    29761 cacaatgcct gatgcgatgc tgacgcatct tatcaggcct gcggggtgtg tagcgggtgt
    29821 aggccggata aggcgcgtca agcgtcgcat ccggcaattg caccgcgcca ctggcggatg
    29881 cggcgattaa tccagttgcg taatatcaaa agcggcgcgg tcgatcaccg caatgatctt
    29941 ttttatttgt gcatcactga tatcgctctg gttgacgcgt aaatccagca ccgctttaaa
    30001 attatccagc gcccgcttca tttgcgggtt ctggcgcagc gccgcgccaa cgcaacgcgc
    30061 tttgatgcgt tcttcaatca tctccacctg ttcgcggttt tcttccagcc actgcgcgcc
    30121 ttgttcggtc agcgcaatct gcttcttacc accttcctct tcgcggatgg taatcagcga
    30181 ctgctcctgc agaaaatcca gcgtcgggta gatgacgccc gggcttgggg tgtaattccc
    30241 ctgggttaga ttctcaatcg ctttaatcaa ttcgtaaccg tggctgtcat cgcgcgagag
    30301 aatatccaga atcaccagac gcaattcacc gtgaccaaag aaacgttgcc gacgaccgcc
    30361 gccgcgaccg tggcgaccac cgcaacattg accgtgttca tgctggtgac cgtgtccgca
    30421 gtgctcgtgt tctgacttct cacctttgca gcagccctca tggcgtggct ggccttcatg
    30481 tttacaacac ccttcgtgat gatggctcat atcagcctcc ttttgcttga ttagatatat
    30541 ctaatttata tctgatgtga gcattactgc aagtacaaaa atgattttta aacgtggaag
    30601 cacctcacat ctaggccgga taagacgttc acgccgcatc cggcagcatt gctatggcac
    30661 tgaatgcctt cacatacttc agacatatca gttctacaaa tcgcttgcat ttatcatgat
    30721 taacaatcat tatcatttgc gaattttatt tagatatatc tgattaccat cacgaaggcg
    30781 ataacaatga ataacacccc ccgctacccg cagcgcgttc gcaatgatct gcgcttccgt
    30841 gaactgactg tgctacgcgt tgagcgcatc agcgccggtt ttcagcgcat tgtccttggc
    30901 ggcgaggcgt tggacggttt tacatcgcgc ggctttgacg atcacagcaa actcttcttt
    30961 cctcaacctg acgctcactt tgtgccgcca acggtaacgg aagagggcat cgtctggccg
    31021 gaaggaccac gcccaccgtc gcgtgactat acgccactgt atgacgaact acgccatgaa
    31081 ctggcgatcg atttctttat tcacgacggt ggggtcgcca gcggctgggc gataaactta
    31141 cggtggcagg tccgcgcggt tcgctggtgg tgccggaaga ttacgcgtat cagctgtatg
    31201 tctgcgatga atccggaatg cccgcactgc gccgccgcct ggaaacgttg agcaaacttg
    31261 ccgttaaacc gcaagttagt gcgctggtta gcgtgcggga taacgcctgt caggattatc
    31321 tcgcgcacct tgatggtttt aatatcgaat ggctggcaca tgatgagcag gcggtagatg
    31381 cgcgtctggc gcagatgcaa atccctgcca gatgattact tcatctggat aaccggcgaa
    31441 ggtaaagtcg ttaagaattt aagccgccgc tttgaagcgg aacagtatga cccacagcgg
    31501 gtacgtgcag cggcttactg gcacgcaaag taactgatat ataaaaagaa aaaggctgac
    31561 gatttctcgt cagcctttgc tatatctggt ggcccctgct ggacttgaac cagcgaccaa
    31621 gcgattatga gtcgcctgct ctaaccactg agctaagggg ccatggtagc ggattataaa
    31681 gtaactccgt gtcgcaatcc agccattacc gcgcgcctgc tgtttttata aacaatgtat
    31741 tttcaatccg ttatactttt cctggtgatg ttagaaagga gtaaatatgg ttgaggatat
    31801 tttggctcca gggttacggg tcgtgttttg cggtatcaac cctgggcttt catccgccgg
    31861 gactggtttt ccctttgctc atccggcaaa tcgcttctgg aaggtgatat atcaggccgg
    31921 gtttaccgac cgtcagttga agccgcagga ggcacagcat ctgctggatt atcgttgtgg
    31981 cgtcaccaaa ctggtagacc gtc
//
LOCUS       DAJTYF010000036        31999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTYF010000036 DAJTYF010000000
VERSION     DAJTYF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.036"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..167)
                     /locus_tag="OO590_001766"
     CDS             complement(<1..167)
                     /locus_tag="OO590_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6637157.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERV"
     gene            320..883
                     /locus_tag="OO590_001767"
     CDS             320..883
                     /locus_tag="OO590_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637158.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(1159..1581)
                     /locus_tag="OO590_001768"
                     /pseudo
     CDS             complement(1159..1581)
                     /locus_tag="OO590_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(1581..2174)
                     /locus_tag="OO590_001769"
     CDS             complement(1581..2174)
                     /locus_tag="OO590_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR6637159.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            2321..2728
                     /locus_tag="OO590_001770"
     CDS             2321..2728
                     /locus_tag="OO590_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6637160.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(2848..3840)
                     /gene="rpoS"
                     /locus_tag="OO590_001771"
     CDS             complement(2848..3840)
                     /gene="rpoS"
                     /locus_tag="OO590_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6637161.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(3903..5042)
                     /gene="nlpD"
                     /locus_tag="OO590_001772"
     CDS             complement(3903..5042)
                     /gene="nlpD"
                     /locus_tag="OO590_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6637162.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(5182..5808)
                     /gene="pcm"
                     /locus_tag="OO590_001773"
     CDS             complement(5182..5808)
                     /gene="pcm"
                     /locus_tag="OO590_001773"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6637163.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(5802..6563)
                     /gene="surE"
                     /locus_tag="OO590_001774"
     CDS             complement(5802..6563)
                     /gene="surE"
                     /locus_tag="OO590_001774"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6637164.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(6544..7593)
                     /gene="truD"
                     /locus_tag="OO590_001775"
     CDS             complement(6544..7593)
                     /gene="truD"
                     /locus_tag="OO590_001775"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6637165.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICAKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(7590..8069)
                     /gene="ispF"
                     /locus_tag="OO590_001776"
     CDS             complement(7590..8069)
                     /gene="ispF"
                     /locus_tag="OO590_001776"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6637166.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(8069..8779)
                     /gene="ispD"
                     /locus_tag="OO590_001777"
     CDS             complement(8069..8779)
                     /gene="ispD"
                     /locus_tag="OO590_001777"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6637167.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(8798..9109)
                     /gene="ftsB"
                     /locus_tag="OO590_001778"
     CDS             complement(8798..9109)
                     /gene="ftsB"
                     /locus_tag="OO590_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6637168.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(9303..9626)
                     /locus_tag="OO590_001779"
     CDS             complement(9303..9626)
                     /locus_tag="OO590_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6637169.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(9676..10281)
                     /gene="cysC"
                     /locus_tag="OO590_001780"
     CDS             complement(9676..10281)
                     /gene="cysC"
                     /locus_tag="OO590_001780"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6637170.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(10281..11708)
                     /gene="cysN"
                     /locus_tag="OO590_001781"
     CDS             complement(10281..11708)
                     /gene="cysN"
                     /locus_tag="OO590_001781"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6637171.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(11710..12618)
                     /gene="cysD"
                     /locus_tag="OO590_001782"
     CDS             complement(11710..12618)
                     /gene="cysD"
                     /locus_tag="OO590_001782"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6637172.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            12870..13907
                     /gene="iap"
                     /locus_tag="OO590_001783"
     CDS             12870..13907
                     /gene="iap"
                     /locus_tag="OO590_001783"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6637173.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(14053..15144)
                     /locus_tag="OO590_001784"
                     /pseudo
     CDS             complement(14053..15144)
                     /locus_tag="OO590_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(15338..15490)
                     /locus_tag="OO590_001785"
     CDS             complement(15338..15490)
                     /locus_tag="OO590_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6637174.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(15754..16488)
                     /gene="cysH"
                     /locus_tag="OO590_001786"
     CDS             complement(15754..16488)
                     /gene="cysH"
                     /locus_tag="OO590_001786"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6637175.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMVEEETRFFGLKRECGLHEG"
     gene            complement(16562..18274)
                     /gene="cysI"
                     /locus_tag="OO590_001787"
     CDS             complement(16562..18274)
                     /gene="cysI"
                     /locus_tag="OO590_001787"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6637176.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(18274..20073)
                     /gene="cysJ"
                     /locus_tag="OO590_001788"
     CDS             complement(18274..20073)
                     /gene="cysJ"
                     /locus_tag="OO590_001788"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6637177.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            20392..20754
                     /gene="queD"
                     /locus_tag="OO590_001789"
     CDS             20392..20754
                     /gene="queD"
                     /locus_tag="OO590_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6637178.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            20832..22103
                     /locus_tag="OO590_001790"
     CDS             20832..22103
                     /locus_tag="OO590_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6637179.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            22094..22354
                     /locus_tag="OO590_001791"
     CDS             22094..22354
                     /locus_tag="OO590_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6637180.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            22371..22946
                     /locus_tag="OO590_001792"
     CDS             22371..22946
                     /locus_tag="OO590_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR6637181.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(23094..23954)
                     /locus_tag="OO590_001793"
     CDS             complement(23094..23954)
                     /locus_tag="OO590_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6637182.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIDVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(23951..24730)
                     /locus_tag="OO590_001794"
     CDS             complement(23951..24730)
                     /locus_tag="OO590_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6637183.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTLVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(24708..26117)
                     /locus_tag="OO590_001795"
     CDS             complement(24708..26117)
                     /locus_tag="OO590_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6637184.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMSGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SIFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(26139..27593)
                     /locus_tag="OO590_001796"
                     /pseudo
     CDS             complement(26139..27593)
                     /locus_tag="OO590_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(27663..28449)
                     /locus_tag="OO590_001797"
                     /pseudo
     CDS             complement(27663..28449)
                     /locus_tag="OO590_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            28768..30045
                     /locus_tag="OO590_001798"
     CDS             28768..30045
                     /locus_tag="OO590_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6637185.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            30072..31542
                     /locus_tag="OO590_001799"
                     /pseudo
     CDS             30072..31542
                     /locus_tag="OO590_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         7801 a         8428 c         7891 g         7879 t
ORIGIN      
        1 acgcgttcgt attttttgtt tggtgtgcga actaccgctt tcagtgaatc ctgagcataa
       61 ccagcatgga gtgaaagctc ccggaaggaa gaccacccgt tatctttgag cgccagctga
      121 atcttcagcg aaggccaatc gcggtcacta tcttctcttt gtctcatctt tcctttaacc
      181 tacaaaataa cccgcgcgga tatccgtatg gataaagatt aagataccaa agattcccta
      241 taaagggttc tttggtgctt ttatggttta ttttttaatt tcattattta tcaatgaatt
      301 agatagaaag gaaccaagaa tgagaaaagc accccaaggt tcttttcctg ccgatggaaa
      361 agaaccgatt atcgaaagaa tctttaggtt agtagaacga tacccttcaa gaaatgcggc
      421 tgctcgggca tgggggatca atgaaggcac tttaaaaaat tattacaaca ggcgggatta
      481 cgcaccgata ccacgcccgc atcagttaaa aaagatagca gaaagcgaaa atgttacctt
      541 ggaatggctc atgacgggag agggtgatgt tgaactaaaa gattccaaag aacccaaaaa
      601 agagaatgcg gataatgttg atgctcaaat tctaatgttc ttgtcatttc ttaaaccaca
      661 agaaaaacaa caagtagctg atgttcttgg gagaaaagga gcggagcaat tactcattct
      721 tctggatgag cacatccaag agttgcacgc tctagttgga gttcgtagag ccatagcgtt
      781 aagccttggt aatttgccag acgaaaaggt tagagagatt tacgcgttgt ccgaagctaa
      841 gggcgatcac ttaaatctac ctcagaaagc agccagcgca taacccagtg caagtgccta
      901 gctgtgctaa aattacattg gttttggcgc ttgcacccat cagcgctata ataaacacct
      961 gaatcgtttt tgaacggtct ttaaaggcta ttgtaagaac ctgtgagtta tcttaaatgt
     1021 tttattgata tcgaaccgtt taacattttt ggactcgctt tatcttgaaa accttgagtt
     1081 cctaacttgt tgattccaat ctattcccgt cttttttaat ctcttcccgg ttatcttgaa
     1141 taaattacct agtcatacga gatagaaacc gccatcgcca tcattcaggc gaaacagcgg
     1201 cagaatatcg aacaggttga tctcttcgcc atcgacggtg ttctgcgccc aggctggatt
     1261 ggcgcggcgc tccggggcaa tcgggaagtt atcccagcgg cggataaact catcaatctg
     1321 ctttttgacc ggggtgtttg gtggcaggcc aagggaaatc gcgtggttct gccaggaacc
     1381 gatggtattc atcgccacgc gggcatcggt aaagccgcga atattatcaa accacagcgc
     1441 gggcgcgccg tcgccgatac gcccggtggc gttagctgct gctgccagat ccggctcagc
     1501 gttcacttct tcgctgattt tcagtaactg gccgtggtca tcaagcgcct gtaaaaagct
     1561 gcgtaaatca tcaaatgcca ttattcattc tcctgagaaa aattccgggc ctgcggcaat
     1621 ccttgccagc gcctggcgtg gggatgttcg aggccaaatt gatccagcac gcgggctacc
     1681 acatggtgga caatgtcatc taccgtttcg ggatggttat aaaaggcagg catcggcggc
     1741 accatcgcca cgcccatgcg tgaaagtgcg agcatatttt cgagatggat ggtgctaagc
     1801 ggcatttcac gcggcaccag caccagtttg cggccttctt tgagcacgac gtccgccgcg
     1861 cgccctacca ggccatcggc gtaaccagcg cggataccgg cgagcgtttt catactgcac
     1921 ggaataacga tcatgccgtc agtacgaaaa gaaccggatg agatgatcgc cgcctgatcc
     1981 gccgggttat ggctgaagtc tgcgagggca gcaacatcgc gagcgctgta aggcgtttct
     2041 agttcaatga tggttttcgc ccacttcgac atcaccagat gagtctcgac attcggcatc
     2101 tcacgcagcg cttgcagtaa tgccacacca agaggcgcac cggtagcccc tgtcatcccg
     2161 acgatcagtt tcattgctta ttctcttaat tgttcgtata cgaacattat tattaacata
     2221 ctccttcatt tccacagcgg caaggattga aaagtaacaa catcttcttc gtcactaaaa
     2281 aaccgctatg atagcgacag agaattagcc cggagtttac atggcgttac gaaataaagc
     2341 gtttcatcag ttacgacagc tttttcagca gcacacggct cgctggcagc acgagttacc
     2401 tgacctgacc aaaccacagt acgcggtgat gcgcgccatt gctgataaac ctggtattga
     2461 acaggtggca ctgatagaag cagcagtcag caccaaagca acgctagcag aaatgttggc
     2521 aagaatggaa aatcgtggct tagtcagacg tgaacatgac gtcgctgaca agcggcgacg
     2581 ctttgtctgg ctgactgccg aaggagaaaa gctacttgct gcggcgatac ctattggtga
     2641 cagcgtggat gaggaatttt tggggcgttt gagtggagaa gagcaggagc tgtttatgca
     2701 gttggtgcgc aagatgatga gcacatagaa tgctgattgc cgggtaggac gctgacgtgt
     2761 cttatccagg cgacaaaaac acaagcttcc caaacaaaag aaaaaggcca gcctcgcttg
     2821 agactggcct ttctgacaga tgcttactta ctcgcggaac agcgcttcga tattcagccc
     2881 ctgcgtttgc aggatttcgc gcaaacggcg caggccttca acctgaatct ggcgaacacg
     2941 ttcacgggtg aggccaattt cacgacctac atcttccagt gttgccgctt cgtaccccag
     3001 caaaccgaat cgacgtgcca gtacttcacg ctgtttggcg ttcagctcga acagccattt
     3061 gacgatgctc tgcttcatat cgtcatcttg cgtggtatct tccggaccgt tctctttttc
     3121 atcggccagg atgtccagca acgctttttc ggaatcacca cccagcgggg tgtctaccga
     3181 ggtaatgcgc tcgttaagac gaagcatacg gctgacgtca tcaactggct tatccagttg
     3241 ctctgcgatc tcttccgcac ttggttcatg gtccagctta tgggacaact cacgtgcggt
     3301 tcgcaggtaa acgttcagct cctttacgat gtgaatcggc aaacgaatag tacgggtttg
     3361 gttcataatc gcccgttcaa tcgtctggcg aatccaccag gttgcgtatg ttgagaagcg
     3421 gaaaccacgt tccgggtcaa acttctctac cgcgcggatc agccccaagt tgccctcttc
     3481 gataaggtcc agcaacgcca gaccacgatt gccataacgg cgggcaattt ttaccaccag
     3541 acgcaagtta ctctcgatca tccggcggcg agaggcgaca tctccacgca gtgcgcgacg
     3601 cgcaaaataa acttcttctt cggccgttaa cagtggtgaa taaccaatct caccaaggta
     3661 aagctgagtc gcgtccaaca cacgctgtgt ggctccctgc gataacagtt cctcttcggc
     3721 caaatcgtta tcactgggtt cctcttctac taaggccttt tcgtcaaaaa cctcaactcc
     3781 gttctcatca aattccgcat cttcatttaa atcatgaact ttcagcgtat tctgactcat
     3841 aaggtggctc ctacccgtga tcccttgacg gaacattcaa gcaaaagcct ggttccgccg
     3901 atttatcgct gcggcaaata acgcagcggg tttacggatt tccccttgta acgaatttca
     3961 aaatgcaagc gtgttgaact ggttccggtg ctacccatgg ttgctatttt ttgccccgcc
     4021 ttcacttctt gttgttcccg gaccagcatt gtgtcgttat gggcgtaggc actcaggtaa
     4081 tcatcattat gtttgatgat aatcagatta ccgtagccgc gcagcgcgtt accagcataa
     4141 acaacgcggc catctgcggt cgcgataatt gcctgtcctt tgctgcctgc gatatcaatc
     4201 cccttgttgc ccccctcaga agcgccaaag gtttcgatca ctttgccctc agtcggccag
     4261 cgccaggtgg agataggcgt actggttgat gtactgctga cagtcggctc ggttgtgctt
     4321 gctgttggta ccgttacagg cgctgtgacc gtggtcgcag ttggcttgtt gttcggcaac
     4381 attttgttag cactctgttc acccgaagac tcagaatacg taattgtcgg ttgcgacgca
     4441 acagcaacgg tggaattttg tgcaggcttg atcacaactc cttgctctgc tgcgtcggcc
     4501 tgggtaatag catttccgcc agtgattggc gtaccggaag cattacccac ctgcaaggtc
     4561 tgaccaacgt tcagcgcgta tggtgcctga atattgttgc gctgagcaag gtcacggaaa
     4621 tcgttgccag taatccaggc gatatagaaa agtgtgtcgc cttttttcac ggtataggta
     4681 ctgccgctat aactgccttt cggaatgttc ccatactgac ggttatagac gatgcgtccg
     4741 ttttccatct gtaccggctg ctgagctact ggctgcactg gctggatttg cggttgttga
     4801 gtagcctgaa tttgtggctg ctgtaccggc tgaatttgcg gttgctgcgc tgtagacgtc
     4861 gtccctattt tcggcggcgg cgtaatcaac ataccagaat tagtatttgc aggcgcattg
     4921 ccattaacgg agctgaccgg tgccggtgga tttgaagtgt cagaacagcc tgccagccat
     4981 agcgaaacca gtgacaaagc cgcaatgcgg cgaacggtga attttgggct tcccgcgctc
     5041 atttatcccc caggaaaaat tggttaataa ccagtgacat aattaccgtg caaggcaccc
     5101 tactgaacac tggaaaagat gttcacgata cgctgaccta cggcaaaata accaggaaaa
     5161 atccaggtat ttcctcacgt tttaagccag ctcacccttc actaaaggga caaagcgcac
     5221 ggcctccacg gtatcgataa taaattcgcc tccccgacga cgcacccgtt tcaaatactg
     5281 gtgctcctcc cctacgggta agacgagaat cccgccttcg tccagctgcg tcattagcgc
     5341 agttggaatt tccggcggtg ccgccgtaac aatgatagcg tcaaacggcg cacgtgcctg
     5401 ccaaccttgc catccatcac catgacgggt tgaaacatta tgtaaatcaa gatttttcag
     5461 gcggcgacgt gcctgccact gcaagccttt aatccgttca accgagcaaa catgctggac
     5521 aagatgcgcc aggattgccg tttgatatcc cgaaccagtg ccaatttcca gcacccgcga
     5581 ctgcggcgtc agctcgagta attcggtcat tcgcgccacc atatacggct gcgaaattgt
     5641 ctgcccctga cctatcggca aggcgatatt gtcccaggct ttttgttcaa acgcttcatc
     5701 aacgaatttt tcacgcggca cggcggcaag tgcattcagc acctgctcat cctgaatacc
     5761 ttgcgcacgt aattgatcca gaagtgcttg tacgcgtctg cttaccattg cgtgccaact
     5821 cccacgctgt ttaaccagtc tgaaaccaca tcttgcgcgc tatgcgcagt taaatccaca
     5881 tgcagcggcg tgatggagac atagccctca tctaccgcag caaaatcggt ccccggaccg
     5941 gcatcacatt taccgcccgg cgggccaatc cagtacagcg tattgccgcg cggatcttgc
     6001 tgcgggatca cctgatctgc cggatgtcgt gtaccgcagc gcgtcacgcg aatacctttg
     6061 atttgatcca agggtaaatc cggaacgtta atattaagaa tacgtccggt gcgcagcggc
     6121 tctttacaca gtgcgcgcaa aattgaacag gttaccgccg cggcagtgtc gtaatgttta
     6181 tgcccgtcaa gcgagacggc cagcgccgga aaacctaaat gacggccttc catcgcggcg
     6241 gctaccgtac cggaataaat aacatcatcc cccagattcg gcccggcgtt aattccggac
     6301 acaacaatgt ccgggcgcgg acgcatcaga gcattcacgc caagatagac acaatcggtc
     6361 ggggttccca tttgcacagc aatatcacca ttttcaaagg taaacgtgcg cagggaggat
     6421 tccagtgtca gagaatttga agcgctgctg cggttacgat cgggggcgac cacctgaacg
     6481 tcagcaaact cacgcaaggc tttcgccagc gtttgtatac cgggtgcatg taccccgtca
     6541 tcattactca gcaatatgcg cataatcacc tgttgtgttg ataagttccc tgacaacgct
     6601 ggttgcaaaa ctacccgccg gaagccagaa acggatctct acggtgacgt catcccacca
     6661 gttccagctt aattgttgcg gatacagcag catcgctctg cgcgcggctt caactttttc
     6721 gcgcaccagt aaagcttgta attcagtttc tgcggcgaca gctgcttgtt cgaatgccag
     6781 cgcttcacgc tgagttcccc attcgccact gcccggcaat gcggcggtta tcatcagctc
     6841 tttatcgttg acgcgacgct gtaattccgc cagttcttcg gtggttgcta caaaccagct
     6901 accacgtccg gctaattgta gcgcatcgcc gtcaacaact tgattaacgt ctgctttttt
     6961 gaggcgctca gcaacaatct gattaaacaa cgcactgcgg gctgccgaca accaaaaact
     7021 ccgtttattg cgatcgcgca ccggagtatt ggtttgcgcc cagcgcagcg cgccctgcaa
     7081 gttgctaccg ccaatcccaa aacgttgggc accgaagtag ttcggtacac cttttgcgca
     7141 aatatcgatc agacgttgtt caacgtcatc gcgattgctc acttcgcgca gaaccagggt
     7201 aaaggcgtta cctttcagcg cccctaaacg cagcttgcgc ttgtgccgcg catactccag
     7261 cacctggcag ccttccagtt gaaaggcgct cagatcgggc atttccttgc ccggcacgcg
     7321 agcacataac cactgttccg ttacagcatg tttgtctttt tgcccagcga agctgacttc
     7381 acgggaatga attttcagga atttcgccag tgcatccgcc acaaaacggg tattgcagcc
     7441 gtttttgagg attctaacca gaatatgctc accttcacca tcaggctcaa agcccaaatc
     7501 ttccaccacc acaaagtctt ccggattggc tttcagcagc ccggtgcctt gcggtttacc
     7561 gtggaggtaa gtgagattat caaactcaat cattttgttg ccttaatgag tagcgccacc
     7621 gcttcacagg caatcccttc cccacgtccg gtaaatccaa gtttttccgt agtagtggct
     7681 ttcacgttaa catcatccat atggcagccg agatcttcgg caataaacac gcgcatttgt
     7741 ggaatgtgcg gcaacatctt cggtgcctga gcgatgatag tgacatcgac gttgccaagg
     7801 gtataaccct tcgcctgaat acgacgccag gcttcgcgta gcagctcgcg gctgtcggca
     7861 cctttaaatg ccggatcggt atccgggaac agtttgccga tatcccccag cgccgccgcg
     7921 ccaagcaatg catcggtcaa cgcatgaagc gccacgtcgc catcagaatg cgccagcaat
     7981 cctttttcgt aaggaatgcg tacgccacca atgataattg ggccttcacc gccaaaggcg
     8041 tgtacgtcaa aaccgtgtcc aattcgcatt atgtattctc ctgatggatg gttcgggtga
     8101 ggtaaaactc ggccagtgcc aaatcttccg ggcgcgtgac tttaatgtta tccgcgcggc
     8161 cttcgaccaa ctgcggatga aatccgcaat attccagcgc cgaggcttcg tcggtaatgg
     8221 tagcaccttc atttagagcg cgcgtcagac agtcatgtaa cagctcacga gggaaaaatt
     8281 gcggcgtcag cgcgtgccat aagccgttgc gatcaacggt atgagcaatg gcatttttgc
     8341 ccggttcggc acgtttcata gtatcgcgca ctggtgcggc gaggatccct cccgtgcggc
     8401 tggtttcgct caacgccaac aatcgcgcga ggtcatcctg atgcaaacaa ggacgagcgg
     8461 cgtcatgcac caatacccac tgcacatcgc cagcggcttt cagacctgcc agcacggaat
     8521 cggcacgctc atcaccgcca tctacaacgg tgatttgcgg atgattcgcc agaggaagtt
     8581 gtgcaaaacg gctatcgcca ggacttatgg caatgacgac acgtgtcacc cggggatgcg
     8641 ccagcagcgc atgcaccgag tgttcaagaa tggtttgatt accgattgag agatattgct
     8701 taggacattc cgtttgcatt cgacggccaa atccggccgc cggaaccacg gcgcaaacat
     8761 ccaaatgagt ggttgccatg ttaattcccg ggctgattta tcgattgttt tgccccgcag
     8821 actgtgcgcg cttcgacgcg tcaggcacca gacgataaaa agtttcgccc ggcctggtca
     8881 tgctgagttc attacgcgca cgctcttcga gcgcctcctg gccgccattg agatcgtcaa
     8941 tttcggcaaa aagttgatcg tttcgcgctt taagtttcgc gtttgtagct tgcagtgccg
     9001 ccacatcatc attgacgcgg gtatagtcat gtataccgtt cttaccgaac cacagcgaat
     9061 actgtagcca gaccagaata gccagcaaca gcagcgttag tttacccatc ctgccccctg
     9121 aaaaactgca tcatcatccc atgcatccga agacgactct acatcctctg ttggggatac
     9181 cgcgacaacg cgggcaaatg taccacattt gtccattgtt acgtataccc agggcgtgca
     9241 gaacataatc tcattattag ttacggtttg aattatgaac agaggagaca agaaagtaca
     9301 aattagccca gtagccacat aaacagtgcg ccaaacataa tgcctactgt caccagggtg
     9361 aaaacaatac tgtagcgtag ctttccgtcc atcaatgaat gcagcgcaat ccccaccact
     9421 accgcgacag gcatcagtgc cagaaagaaa ggccaggtgt agataaagaa gaacagcgtg
     9481 ttagagccat aaatcaacat cggcatcgcc agcgcaaata accaggagat aaaaccgacc
     9541 acggcaccag gcagtgacca tgtagtttct tcatcctcag taaggctgtc gttatttgtt
     9601 agtgtaatgt tatggctatt acgcatattt gatcctgtta ctttgacgaa ccgggcattg
     9661 aaacccggtg gtgtctcagg atctgataat atcgttctgt ctcaacagat ctaataattg
     9721 ctgtaccaaa tttgttacta attgttcacc attgagatga atttctgccg attcaggctc
     9781 ttcgtaaacg gaatctattc ccgtaaagtt gcgcagttca ccggcacgcg ctttcttata
     9841 taagcctttt ggatcgcggg cttcgcaaat cgccagcggc gtatcgacaa acacttcaat
     9901 aaagcgccct tctcctacgc gttcgcgaac catctggcgt tcggcgcggt gtggcgagat
     9961 aaatgcggtc agcaccacca gtccggctgc aaccatcaaa ttcgccactt caccgacgcg
    10021 acggatattc tctttacgat cggcatcgct aaaaccgaga tcgctgcata atccgtggcg
    10081 aacattgtcg ccatccagca gatacgtact gacgccgagt ttatgtaacg cctcctccag
    10141 cgccccggcg accgttgatt taccggaccc ggagaggccg gtaaaccaca gcactacacc
    10201 acgatgaccg tggtgtagtt cgcgttgttg cacagtgacc ggatgactat gccagacgac
    10261 gttttcgtca tgcagcgcca ttatttatcc cccagcaaat cgcgcgcgcc ccagtgcgga
    10321 aagtggcgac gaaccagggc attcaattcc agttcgaatg cactgaattc agatgacgca
    10381 gctgttgcct ggctaactgg ctcgtgcacc ataccggcac ccacggtcac attgctcagg
    10441 cgatcgataa aaatcagccc accggtaacc ggattttgct gatagcgatc taacaccagt
    10501 ggctcgtcaa aagtgagatc caccaggccg atgccgttca gcggcaggtt ttcaacttcg
    10561 cgttgggtaa ggttattaat atcaacctga taacgaatgc catcaacacg agcacgcgtc
    10621 ttcttaccgg caattttgat gtcataactc tggcccgggg aaagcggctg ttccgccatc
    10681 cataccacat ccaccgacgc actctgcacc gccggtaacg cttcgtctgc cgccagcagc
    10741 agatcaccac ggctgatatc gatctcatcc gtcagcacca gggtgatcgc ttctccggca
    10801 aagacttctt cgcgatcacc atcaaaagtc acgatccgcg cgacgtttga ttccacacca
    10861 gagggcagca cttttacacg ttgcccgact tccacgcgac cggatgccag cattccggcg
    10921 tagccacgaa aatcgagatt tgggcggtta acgtactgca ccgggaagcg cattggctgg
    10981 gcatccacca ctcgctggat ctccacggtt tccagcactt cgagcagtgt cagaccgctg
    11041 taccacggca tactttcact ttgcgaagcc acgttgtcac cttccagtgc ggagagcggc
    11101 acaaagcgga tatccagatt acccggcagc tgcccggcaa aggtcagata atcttcacga
    11161 atacgggtga acgtctcttc actgtaatcc accagatcca ttttgttgat cgccacgacc
    11221 agatgtttga tccccaacag tgtggagata aaactgtgac gacgggtttg atcgagcacg
    11281 cctttacggg catcaatcag taagatcgcc agttcacatg tcgatgcgcc agtcgccata
    11341 ttgcgagtgt actgctcgtg ccctggggtg tcggcgataa taaatttacg cttctcggta
    11401 gagaaatagc ggtaggccac atcaatagtg atgccctgtt cgcgctcagc ttgcaggccg
    11461 tccaccagca gagccagatc cagcttttcg ccctgggtgc cgtgacgctt actgtcgtta
    11521 tgcagcgatg agagctgatc ttcgtagatt tggcgggtat cgtgcagcag acgaccaatc
    11581 agggtacttt tgccgtcatc gacgctacca caggtcagaa aacgcagcag gcttttatgt
    11641 tgttgcgcaa tcatccaggc ttcgacgccg ccttcattgg cgatttgttg tgcaagtacg
    11701 gtgttcatct taaaaatacc cctgacgttt tttcagctcc atggatcccg cctggtcgcg
    11761 gtcaatcacg cgaccctgac gttcactggt ggtggaaacc agcatctctt cgatgatttc
    11821 cggcagtgtt tgtgcatttg actccaccgc accggtcagc ggccagcagc ccagcgtacg
    11881 gaaacgcacc atccgttttt tgatcacttc gcccggttgc aggtcgatac ggttgtcatc
    11941 aatcatcatc aacataccgt cgcgttccag aaccggacgt tccgcagcga gatacagcgg
    12001 cacaatgtcg atattttcca gccagatgta ttgccagata tcctgctcgg tccagttaga
    12061 gagcgggaag acgcggatgc tttcgccttt gttaatttgc ccgttgtagt tgtgccacag
    12121 ctccgggcgc tgatttttcg gatcccagcg atggaagcgg tcacggaaag agtagattcg
    12181 ctctttagcg cgggatttct cttcgtcacg gcgcgcacca ccgaaggcgg catcaaaacc
    12241 gtatttgttc agcgcctgtt tcaggccttc agttttcata atatcggtat gtttcgcgct
    12301 gccgtgcacg aatggattaa tccccatcgc cacgccttcc gggtttttat gcaccagcag
    12361 ctcgcagccg taggctttcg ccgtacgatc gcggaactca tacatctcgc ggaatttcca
    12421 gccggtatcg acatgcagca acgggaaagg cagcgtacct ggataaaacg ccttgcgcgc
    12481 cagatgcagc atgacgctgg aatctttacc gatagagtag agcatcaccg gatttgagaa
    12541 ttctgccgcc acctcgcgaa taatgtggat gctttccgcc tccagttgcc gcaggtgagt
    12601 aagtcgtttt tgatccataa ccgttccttt gcaataccgc tattttcttg ccatcagatg
    12661 tttcgactat agggagcgta agagaacgaa tgaaattacc aattagaatg agtagttcct
    12721 taacggaata acgatttggc aaagctaata tcaaaaagtg cttaaggcac cggatttcgg
    12781 gcgtttagga agatttgaaa ttgttttagc gcagcggcag tttcatacta tggcggtaaa
    12841 aaaatttgca tggtatttaa ggactcacta tgttttccgc attgcgccac cgtaccgctg
    12901 ccctggcgct cggcgtatgc tttattctcc ccgtacacgc ctcgtcacct aaacctggcg
    12961 attttgctaa tacccaggca cgacatattg ctactttctt tccgggacgc atgaccggaa
    13021 ctccagcaga aatgttatct gccgattata ttcgccaaca gtttcagcaa atgggttatc
    13081 gcagtgatat tcggacattt aatagtcggt atatttatac cgcccgcgat aatcgcaaga
    13141 actggcataa cgtgacagga agtacggtga ttgccgctca tgaaggcaaa gcgccgcagc
    13201 agatcatcat tatggcgcat ctggatactt acgccccgct gagcgatgct gacgcagatg
    13261 ccaatctcgg cggactgacg ttacaaggaa tagatgataa cgccgcaggt ttaggtgtca
    13321 tgctggaact ggcagaacgc ctgaaaaata cgcctaccga gtatggtatt cgatttgtgg
    13381 cgaccagcgg cgaagaggaa ggaaaattag gcgctgagaa tttactcaag cggatgagtg
    13441 acaccgaaaa gaaaaatacg ctgctggtga ttaatctcga taacttaatt gttggcgata
    13501 aattgtattt caacagcggt gtaaaaaccc ctgaagcagt aaggaaatta acgcgcgaca
    13561 gggcgctggc aattgcacgc agtcacggaa tagccgcaac gaccaatccg ggtttgaata
    13621 aaaattatcc gaaaggcact gggtgttgta atgacgcaga aatattcgac aaagcgggca
    13681 ttgctgtact ttcggtggaa gcgactaact ggaatcttgg aaataaggat ggttatcagc
    13741 aacgcgcaaa aacacctgcc ttcccggcgg gaaatagctg gcatgacgta agactggata
    13801 atcaacaaca tattgataag gcacttccag gaagaataga acgtcgctgc cgtgacgtta
    13861 tgcggataat gctaccgttg gtgaaggagc tggcgaaggc gtcttgatgg gtttgaaaat
    13921 gggagctggg agttctaccg caggggcggg gaattctaag tgatacccat catcgcatcc
    13981 agtgcgtccg gtttacttcc gggaaaaccg caccgtattg cgccggttta tctccgctgg
    14041 agcggggaac acctctggcg aatagaaagc aagccaggtt ttgaccagtc gatcgtacat
    14101 ggcattggcc gttgccattg ttggcaggcc aaaaaacagc ccctgagcgt tacccgcagc
    14161 catcaacctg tgcgccagga taagcgccgc ttctgtttta cctgcgccgg tcacatcttc
    14221 cagaataaac aactgtggtc ctggctgatt gatatccaga tccagcacct tttgctgtaa
    14281 cggtgtcgta ttctcaataa aaggaaacag cgtattaatt ccggtgaaag gcgcggtttc
    14341 tgcttgtggc ggaaagacgg ttaatgcgtt ttgagcctga actaaagcct tttgccagta
    14401 atcttcaata tctattgcct gtgcgacgcg tggaaaaaat cgcgttgacg accctgtcca
    14461 gtctgcaagt acgacggttg cagagatata ccaggaaagt tgttttataa gttcaacgcc
    14521 ctcgtcatca tcccagaatg tggggatctc tattcgcgga aacagtggct tgatttcaag
    14581 gagaaaatct cgtgctgcag ctttgtcttc aggcagaaaa ttatccaatt catccatacg
    14641 atcagggggc cgaccatgat gccctgtcgt cacgggcatc cacatttcta tcactcgcct
    14701 aagtttgcgc ggagagagcg aagatgaagg aagtaactcc tgacattcac ttaaataatg
    14761 attccacaac cagtagccca gcgttgaatg agagattttt tcgtaatttt ttctggcacc
    14821 ttcctgaatc ttgaattcag ggggcaggta aagttgttga aaagagcggg caaattttcc
    14881 aatatcgtgc cagcacagca accaggcgaa aaattgagct gcctgttcct tgtcagaaat
    14941 ccctaattga cgaaaataat cagccagccc gaagcaattt cttttaacca ttaaatagcc
    15001 cattgcggcc acatccagca aatgccagca aagaaggtga tagtcgtcgc taccctcttt
    15061 ctcgccacgt cggttttttc cccagaaatc aaagaaagtc acaatatttt tatccttcag
    15121 taaacttaaa ggatatttac gcatatttta aaaactatct gtgatatata tcagccaata
    15181 aaacatttac attatgaata aattaatgat tttcatttga aattcataat aataaataga
    15241 gactatatat tcacataaaa aatataaatt ttaaaaaagg gtgcaggaga ccaaaccatc
    15301 acaattcagt acgcggggtt ttacccccgc gtggtggcta cttcttcgat tcgtaagcga
    15361 gaactgcctt aaactccata ccacgttctc tgatactcaa ctcacataac gagtcaccta
    15421 ccatcaacgt gaatcccagt actgtacaac acagtacgat gattgccaca agggcatatt
    15481 ttgtcagcat gtcttgcctc cctgcaaaga agagactaac atccgaattg ctacgttcga
    15541 acgcgagcct caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg
    15601 aatgcttaac gccagacagc ctcaagcacc caacgccatt ctatacctgt taattttccc
    15661 tgcgatgttc acctttaaat gccggaatgc tgctcgcaaa ataaaaacgc ccccatcatt
    15721 tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgtaatc cacattccct
    15781 ttttaagcca aagaaacgcg tttcttcttc caccatgccg ggttcccatt tacgggttgt
    15841 atgggtatcg cccaccgata aatatccttc gtcccataat gggtgatatt tcaggccatg
    15901 tttttgcagg tactgataaa tagttcggtt atcccagtcg ataatcggca gcactttaaa
    15961 tacgccacgc tgaattgcca gcaccggtaa attggcacgg ctgccggatt gttcacgccg
    16021 caggccagca aaccacgttt gcgcattcag ttctttcagc gcccggttca tcggttcgac
    16081 tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgttcccaca gtttgccgta
    16141 gcgtgcttcc tgccaggcgg cgctttcggt agcgcggtac actttcaggt tgagcttgag
    16201 tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacagatagc cggtgtcggt
    16261 gaggatcacc ggaatatccg ggtgaatctg attcaccaga tgcaggctca ccgccgcctg
    16321 aatgccaaag ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggcaac
    16381 gcggccttca gcgtccagtt tttccagttc ggcgttagtt tctgccagcg ccagaatgcg
    16441 atctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact
    16501 gtttgccttg cctgatgcga cgctcacgcg tcttatcagg cctacaagac cgggctgatg
    16561 gttaatccca caaatcgcgc gccggatcga gcaccgggcg aatgatgccc gcacgcaccg
    16621 taaagtcgcc gaagccttca cccgcttcgc gctctttcgc ccagcgccct atcagttcat
    16681 caagcgacgc caggatttcc ggctcggtga tgttttcttt atacatccgt gggatacgtg
    16741 tcccaatgcg gttgccgcca agatgcaggt tgtagcgacc cggtgcttta cccaccaggc
    16801 ccacttccgc cagcatcgcg cgaccacaac cgttcgggca gcctgttaca cgcatcacga
    16861 tatgctcatc gctgacacca tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg
    16921 gcaggaaacg ctccgcttcc gccatcgcca gcgggcaagt cgggaatgac acgcaggcca
    16981 tcgagttttc acgctgcggc gtgacggcat tcattaaccc gctctctttg gcaattttct
    17041 cgatcttcgc tttctcgctt tccggcacac cggcgatgat cagattctgg ttcgccgtaa
    17101 tgcggaaatc acctttgtgg atcttcgcga tctccagcag gccagttttc agcggacgcc
    17161 ccggataatc aaggatgcga ccattttcga taaacagcgt caggtgccag ttatcatcaa
    17221 tgcccttaac ccagccaata cgatcgccgc gtcctgtaaa ttcatacggg cggatcggtt
    17281 caaatttgat ccccgcgcga cgctccactt ccgctttaaa cgtctcaacc cccacgcgct
    17341 ccagtgtgta ttttgttttg gcatttttac gatcggttcg gttaccccag tcacgctgag
    17401 ttgtcacgac ggcttccgcc accgctagcg tatgctctag cggcagatag ccaaactcac
    17461 tcgccgtgcg ggcgtaggtt ttcttgttac cgtgttcgat agaaagccca ccgcccacca
    17521 gcaggttgaa gcccaccagc ttgccgtttt ccgcgatcgc cacgaagttc atgtcgttgg
    17581 cgtgcagatc aatatcgttc tgcggcggga tcactaccgt ggttttgaac ttacgcggca
    17641 ggtaggtctg accaaggatc ggttcttcat cggtggtggc gactttttcc tgatcgagcc
    17701 agatctccgc atacgcgcgg gtacgcggca gcagatgttc agaaatcttc ttcgcccact
    17761 cgtacgcttc tgcgtgcagc tgcgactcgt aagggttcga ggtgcagagt acgttacggt
    17821 tcatgtcgtt agcagtcgcc agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg
    17881 gtttgacgtt ctttttcaga atgccgtgga actgaaacgt ctggcggttg gtcaggcgaa
    17941 tgctgccata gatggtgttt tcaccggcaa atttatcgat cgcctgccac tgtttggtgg
    18001 tgataactcc acccggcaga cggcagcgca acagcatcgc gtggcgcggc tccagcttct
    18061 gttcagcacg ttcggcgcgg atgtcgcggt catcctgctg atacatgccg tggaagcgga
    18121 tcagcaggaa gttatcgccc ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa
    18181 tggtgccgcg caggtagttg ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt
    18241 cgaccactaa aggccctgga tgtttttcgc tcattagtag acatctcgct gataacggcg
    18301 ctctacgcgc agctcactta aaaattcatc cgccgcttcg gtgtccatgc caccaaattc
    18361 ggcaatcact tccagaagtg cctgctcaac gtctttcgcc atgcgattag cgtcgccgca
    18421 gacataaatg tgggcaccgt cattgatcca gcgccacagc tccgcgccct gttcgcgcag
    18481 tttatcttgt acgtaaactt tttctttttg gtcgcgcgac caggcgagat cgatccgcgt
    18541 cagcacaccc tctttgacgt agcgctgcca ctccacctga tacaggaagt cttccgtaaa
    18601 atgcggattg ccaaagaaca gccagttttt acctggcgct tcgtcagcgg cgcgttgctg
    18661 cataaaggcg cggaacggcg caataccggt gcctgggcca atcataatca ccggggtttc
    18721 caggttagcg ggcaggcgga agttatcgtt atgttcgata aatacgcgga cttcgccctc
    18781 ttcttccacg cggtcagcga ggaagctgga ggcaccaccg gcacgggcgc ggccttccac
    18841 gtcgtaacgc accacaccaa cggtgacgtg tacttcgttc tcgacttccg cctgcgagga
    18901 ggcaatggaa tagagacgcg gggtcagcgg gcgcagcaga ttaattagcg cttcggcatc
    18961 aagctgtgcc ggggagaaac gcaccatgtc aacaatcagc gtcgtcgcgg cgtaatgctg
    19021 taacttcgct ttatcgccca ccagcggcag cagtgtttca ctgcgggtaa gcgtggcgta
    19081 attctcaaca atgttggcgg tgttgacggt cagttcgaag tgccactgta gcgcttcgtt
    19141 cagaggcaac gttttgccct cgacagtgac aggttcatcg cctgtcagcc acagcagttc
    19201 gacaagttct ttcaccagtg ccggatcgtt ctgataccag acgcccagcg cgtcacccgg
    19261 ctggtaacgc aggcccgagt cgcctaagtc aatttcgata tggcgaacgt ctttttcaga
    19321 gttacgccca gtaattttct ggttaacaga gaggctagcc accagcggcg cgtctttgct
    19381 gtacgggctg gtgtggattt catttaccgc gccagtagcg acggattgcg aaggtgccgc
    19441 gacaggcgca cgcgatttaa gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc
    19501 ctggtattca acatcagaat cgacacggtc gagcaggcgt tcaccaccca gttccgccag
    19561 cttgctgtca aaatctttcc cggactggca gaaaaattca taagagctat cgccgaggct
    19621 aaacacggca aacgcggtgt tttccagctt tggcgctttt ttggagaaca ggaacttatg
    19681 cagcgcgacg gcttcttccg gcggttcccc ttccccttgc gttgacgtca ctacgatgag
    19741 cagtttttcg ctggcgattt gtttgaattt atagtcgccc gcgttcacca gcttaacgtt
    19801 cagttttgct gctaataaat catcgcgtaa tgcttcagca acccggcgcg cattgccggt
    19861 ttgcgaagca gagataatcg ttatacccgg catttctgcg gcgggcgctg gcgtcactgc
    19921 aagcgcaaca ggctgctggt tgagcacgcc ccagaaatag ccagaaaccc aggcaagctg
    19981 ggtaggcgtt aaatcggtcg tggccgcctg aaggcgtgcc agttgctccg ggttcaacgg
    20041 aagcaacgcg gaaggtggga cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc
    20101 tgtttctgcg ccctgtcagc gcccataaaa cagaagagaa ggtaaggtta acggggtgaa
    20161 cggtgtggat taaagacggg atagcgataa ctaataacca aatcgactaa cctgttttag
    20221 caatagtctt taacaacaaa atagattaac caacctaatg aaaaacaaat gaatttagcc
    20281 aatcattaag ataaatcagc gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa
    20341 cagcctttcc ggtactctac ggcggtttta tcgtcctgta gagaaattat gatgtccacc
    20401 acgttattta aagatttcac cttcgaagcc gctcaccgct taccacatgt cccggaaggg
    20461 cataaatgtg gtcgcctgca cgggcattcc tttatggtgc gactggaaat taccggggaa
    20521 gtcgatccgc atacgggctg gattatcgat ttcgctgaac tgaaagcggc gtttaagcca
    20581 acttatgagc gcctagatca ccattatctc aatgatattc caggtctgga aaacccaacc
    20641 agcgaagttt tagcaaaatg gatttgggat caggttaaac ccgttgtgcc gctgttaagt
    20701 gcggtgatgg taaaagaaac ctgcaccgca gggtgtatct atcgcggcga atgataagag
    20761 tgtgtcggcg gtcaatttcc ctttagtaac gctatgttag ggtgttgtgt tctggatatc
    20821 tggggcatga catggaagac gactgcgaca ttattattat tggtgccggt attgcaggca
    20881 ccgcttgcgc gttacgctgc gcgcgagcgg atttatccgt tttgttactc gaacgcgctg
    20941 aaatccccgg cagcaaaaat ctttccggca ggcggttata tacccatgca ctcgcggaac
    21001 tcctcccgca atttcatctg accgcgcctc ttgaacgacg catcactcac gaaagccttt
    21061 ccctgttaac gccggatggc gcaacgacgt tttccagctt acagcccggc ggtgaatcct
    21121 ggagtgtatt acgtgcacgg ttcgatccgt ggctggttgc cgaagccgaa aaagaaggtg
    21181 tcgaatgcat ccccggagcg acggtggatg cactgtatga agaaaacggc agagtctgtg
    21241 gcgttatttg tggtgacgat attctccgcg cccgttatgt ggtgctggca gaaggtgcca
    21301 acagcgtcct ggctgaacgt cacgggttag tgactcgtcc tgctggcgaa gcgatggcgt
    21361 tggggatcaa agaagtgctg tcgctggaaa catccgctat tgaagaacgt tttcatctgg
    21421 agaataacga aggcgcagcg ttgctgttca gcggcggaat ctgtgatgac ttacccggcg
    21481 gcgcatttct ttatactaat caacaaacgc tctcgttagg gattgtttgc ccgctctctt
    21541 cccttacgca aagtcgtgtt ccggcaagcg agctgctgac tcgctttaaa gcgcatccgg
    21601 cagtgcgcac gcttatcaaa aacacggaat cactggagta tggtgcgcat ctggtgccag
    21661 aaggtggctt gcacagtatg ccggtgcaat acgccggtaa cggctggctg ctggtgggcg
    21721 atgcgttgcg cagttgcgtc aataccggaa tttccgtgcg cggcatggat atggcgctga
    21781 ctggcgcaca ggcggcggca caaacgctaa taagcgcctg ccagcaccgc gagccgcaaa
    21841 atctgtttgc gctttatcat cacaacgtag agcgcagcct gctatgggat gttctacaac
    21901 gttatcagca tgttccggcg cttttgcaac gccctggctg gtatcgggcg tggcctgcgt
    21961 taatgcagga tatttcccgc gatttatggg atcagggtga taaacctgtt ccaccgctgc
    22021 gccagttatt ctggcgtcat ttacgtcgtc atggcctgtg gcatctggcg ggcgatgtta
    22081 tcaggagtct gcgatgtctg tagcccgtaa tctctggcgc gttgctgatg cgccgcacat
    22141 tgttccggct gactccgttg agcgtcagat ggtacaacgg ttgattaacg cctgtccggt
    22201 aggtcttttt tctctcacac aggaaggtgg cttacgtatt gactatcgcg gctgcctgga
    22261 gtgtggcacc tgccgtttgc tgtgcgacga atccacacta caacagtggc gctatccgcc
    22321 ttccggattc ggcatcacct accgctttgg ataaataata aggataattt atgcccctct
    22381 tacacctgct ccgccagaat ccggtgattg ctgccgttaa agataatgcc agcctgcaac
    22441 tggccatcga ttctgaatgc cagtttattt ccgtgttgta cggcaatatc tgcaccatca
    22501 gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc ttttattcat gttgatttgc
    22561 tggaaggcgc gtcaaataaa gaagtggtga ttcagttttt gaaactggtg accgaggcgg
    22621 atggaattat cagcaccaaa gcatcgatgc taaaagcggc aagagcggaa gtttttttct
    22681 gtattcatcg cctgtttatt gttgattcga tttcgtttca caacattgat aagcaagttg
    22741 cgcaatcgaa tccggattgt attgagatcc tgccaggctg catgcccaaa gtgctgggct
    22801 gggtgacgga aaaaatccgc caaccgctga ttgctggcgg gctggtatgc gatgaagaag
    22861 atgcgcgtaa tgcgattaac gcgggtgtcg tggcgctttc caccacgaat accggagtct
    22921 ggacgttagc gaaaaaattg ctttgacggg ataatcgtaa ccaattgaat ttgttttgat
    22981 ttgttggccg cacgccacat ccgacattca gcgcctgatg cgacgcttaa cgcgtcttat
    23041 caggcctaca ggtccccagt ccagaaccgt aggcaggatc atccggcagc acgttattgg
    23101 tattctgcgt gaatgtttgt caccagtgcc tccagcacca ctttccagtc atcaaccacg
    23161 tcaatatctg cctgtgaaaa taccgctgcg ctggcgtcat gattaatcgc caccataaac
    23221 ttgctattgc gtacgcccgc cattaacgct gctgcgccgg aagcgccaac aacaatgcac
    23281 acctcaggtg ccagcagatg tccggaaata ccaatcactt tttcagcatc aacgccaccg
    23341 ttcatcactc gcgcccgact gtagcccact tcggttccca gcttttctgc cagcataacg
    23401 atttcctggt tatctgcctc tccgccctgc cccaccacca gcacccgtct ggcctcagct
    23461 aatgggtcgc ggctaacatt tttcagatcc tctgtgctaa ccagccagtc gggaagcgca
    23521 cctgggacaa tgttcagttg ctgcattcca gagggcaacg tggcgttttt cgctgctccc
    23581 gcctgacgcg ccagggaaag gcataacggg cgtttttctg tttgcagcgt cgccgttaac
    23641 gcatttcccc agtgggattt gcgcatgctt acggttggaa tatctaacga agtcacctga
    23701 cagatgcttg caccatgtaa acgccaggcc agccgggtcg acagttcatc gccaaacgtt
    23761 cccggcggaa agagaacaac gtccgcaggc gttcgctgcc actgttccac catcgcatcc
    23821 agcacctgtt ccgccactac gggctgaggt tctatttgcc agtgcgccag cgtacaaccg
    23881 ctgaaatcct gcgccgccag ccagctcgct attgcggcat tctcctgatt tattgtcaca
    23941 attgcgatat tcatggctgc atcctttggc gtaaatagtc ctgccatagc ttttgagctt
    24001 tttctgctac cgtttgccca tcaatcagcg tcgctccgca tcgttgctct gctcttgcca
    24061 gttgcagaca ctgcatcgct ggcatttcgg cagcaacagt tttacggata atttctgctt
    24121 tccccgcagc cattcgctgg cgcattcccg gtactggcaa ggccacttca ccgcactgac
    24181 gcacggcaat caccgcaggc aagcgaaccc ggcaacagcg caacccatgt tcagtacgct
    24241 gttcgagggt gataaacagc gcgtcgagag tgaaacgctc caccagggtg aagcagggcc
    24301 agcccagcat ttccgccagc aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc
    24361 agccggtaat tatcagatcc agcggattct gacgctgcca ttcggcaata tggcgagcga
    24421 caaattccgg cgcaaagcgc agatctgccg ccgtctccag caatacagct tcctcaaacc
    24481 caagagccat gagatagcgc agccagtgca acgcccgttc atcccccatg ctcaacgcgg
    24541 ttaaagacat cggcgtaccg ttttttctct gcgctaacag cagcgcggca gcggcctgtt
    24601 catcagcacc gagtaaactt cgcagtagcg aaacatccgg tccgctatta ccctgagccg
    24661 ccgcctgcca ctctttttcc gccagcattc cggcatccgg ttcggcttta aacgctaaca
    24721 gaatgttcat tcgcacctcc tacatttccc gccgccacca gcgggagggc tttagtttct
    24781 ggtgcccata gccaggtcac aacaaaacca accaacaaca ctgtcgccag gagcaataag
    24841 gtgacttgca ttccccactg cgccagcacc cacggcagca ggccagtact taccgccgcg
    24901 ccaagtcgac tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa
    24961 ctttccgcag gcaaaatgcc caccagatta ctgactgccg aaatggtggt gctgaagaga
    25021 acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac aggccattac taccagcgtt
    25081 gccgccagca gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga
    25141 actaatccca gcagcgcgcc cacaattaac aacgcattaa gcatcaggct ggcagtcagc
    25201 gcatcttcca gaccaatagt ctgggcgata gttggcagcc aggtataaat cacaaaccat
    25261 gggattacga ggcagacaaa gaagatgctg ttaaacgccg tgcgccgcca gtaacgcgaa
    25321 gagaacaagg ttttgatgtg tttatgggtc gccgttacca cttcatcgcc cagtaaaaca
    25381 tggggaccaa aatagcgatg cacgatagcg tgagcttctg caaaacgccc ctggcacagt
    25441 agccaacgtg gcgattctgg cgttccccag cgtaataacg taatcaacaa cgcgggcaga
    25501 gctgccgatg ccagcaacca gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg
    25561 gcaatacttg ccaacacata gcctacggtc cacaccacgc tgaatgcgcc cagcaaaata
    25621 ccgcgatggc ggcgcgggga aaattcagcc agcaaggtgt gacctactga gtaatcgcct
    25681 cccagaccaa tgccgatcaa aatgcgcagc ccaataagat gctctggcgt ggtggcaaaa
    25741 aattgcaaga acgaagcgag cgtaatcagc aaaaagctga aggtgaagat tttttgccga
    25801 ccaatatggt cggagatcca cccaagaacc aggctgccaa gaaacaaacc aaggagcgcc
    25861 gagccgccgc tcattcccgc cataaacggc gtcagttgca tggcgggcgt aagttgaata
    25921 atggcgtaac caatgacgcc gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc
    25981 gcagcaattc gacagtgaaa acggttaagc ggtaaatcat ccattcgcac cggtgaagtg
    26041 ttcatcttta ccccttcatt gtccggtaat aagaacggct tctcgcctga gaagccgaaa
    26101 aggcaacgcc ccgtcatcgc ggaacgctgc ctgatacatt atttttcaat cggatagata
    26161 gtacccgtat tcataatgcc gttaggatcg aactgctttt tcagcccttc cagcaacgcc
    26221 cacgcgctgc cgtgttccag cttgctccag tgaacgcgat gtttaccaat gccatggtgg
    26281 tgcaccatcg aaccgccgag gcgaatggtt tcttcgcaga tgattttgtt gagcggattg
    26341 tggtacttgt cgatttcctc ttccggctta cagtcaacaa cgttgtagtc atagacgaag
    26401 tacatgttgg tgccgttctg atagctatga gaggagtgac cgcccagcat ggtgatgtcg
    26461 tcggcgtgcg ggaactcagt acgaatacgg ttaataacgc tttcgtagat ttcgtggatg
    26521 cagctccagc agccggacac ttcggtggta aagcccatgt tgccggtttt gaggatctgc
    26581 acacgttcgg cagccacttt atccggtccc cagttcaggt tgttaaacca ggtttcaatc
    26641 agtttgctgt ccacgcgctg gcactgcggg tagcgggcaa cgatttccgc aatcccttcg
    26701 cccgtcgcct tcgcaatgcg agggttacct tcagccataa agatcagcac ccattttccg
    26761 tcggcaaaat gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg agcgatcgac
    26821 ggacgatacc cttccaccat gatttcacgc aggatgttga agccggtttt catgtcttcc
    26881 aggatatagc cgtagaagag gttgttttcc ggggtgtatt taaagatttt cactgttact
    26941 tcagtgatat agcacaatgc accttcgttg ccgatgatga tgtgacgaat gtccgggcca
    27001 gccgcgcggc gtggcacgtt tttaatgcgt gtgacggtgc catctgccaa cactgcctcc
    27061 agaccaacga ccatatcttc gattgcgccg tagagtgtgg agaactgccc gatactgcgg
    27121 gttgctacca ggccgcccat ctgcgccagc ggctttgact gcggagaatg ccccgtggtg
    27181 taaccttttt cacgcaacgc gttttccagc acttccagcg gaacaccaca ttgcgccgtc
    27241 gcctgcatat tctcaatatc aatattaatg atttgattca tggcggagcc gtcgagcacc
    27301 accgagtttt ctacaacagt ttccagccca ccttcggtgg cggaagcacc ggtacgcggc
    27361 acaccgttaa ttttgtgcgc attcataaaa ttcagcacac gggatactta ctctgtggaa
    27421 ccgagtttta cgaccgctgc cggaatcggc aaagtataaa tgccatgaat atccggaaat
    27481 ttacgaaaac ggtcaatact gtttttcttt aatactgttt catcggtaat tacgcgatct
    27541 gcaccaacaa tttcctttag ctggtcgaca atcgctgcgc gagataaaga catagtaatt
    27601 ccttcctgat aataaaaatg gaataatcat taataagaga aaacagaaac caccgttatg
    27661 gattagcgca ctaaataacc gccatcgacc actaataaat gcccgttgac ataattcgat
    27721 gcctgacttg cgagaaacac ggctgcgccc attaaatcct gagtatcgcc ccaacggttt
    27781 gccggaatat gatcaagaac gcgctgattg gtttctggat tactgcgtgt cgccagcata
    27841 atatgctgtt gcataatagc caggggcgat accatttacc tgaatattat attgacctag
    27901 ttcatcacaa taagctttgg tgaacccggc aagagcatgt ttagtggcag aatatgcagg
    27961 tgaccattgt ccacctaagt aagagaacaa tgaacagata ttaataattt tgccgctttt
    28021 ctgcgggatc ataatttttg ccgcttcata gcttaactcg aatgcggcgg tcaggttcac
    28081 atcaatcatc ggatcccagt cggcacgacc gaagtccagc accttattca gcttacaaat
    28141 accggcattg ttaaccagaa tatcgacagt accgaagcgc tcacagcagg cagcgataat
    28201 cttctgcggt gcgccttctg cggtaatatc cacctgcatg aagtccactt caacaccctg
    28261 tttttcaatc atttcctttg tttcgccgtt atctttgacg aagctaggaa taaaaatatt
    28321 tgcgccagct ttggccaacg ccattgcaaa tgcctgacct aaaccgctat tcccgccggt
    28381 aacaattgct gttttacctt tcagggagaa aaaatccatt gagaacgcat tgagagattc
    28441 gattgacata gttagctcca atttccgtaa ggcaaaaaaa aagaaaggta acttcccccg
    28501 cgagagggaa gttacctttc tcattatctc tggtaactgg agataatttt tagcataacg
    28561 gcataactgc taatgtgatc gtaatcacag tgtgatattc gtattgtcaa atagttttaa
    28621 aaagcagaag cgtgattact ctcacacaac acctctgctt tccatgttat ttcttattgc
    28681 aattactaga gacctgagaa aagtaatcca cctgaaaggg cggcttactt ttctcttttt
    28741 tttgtctatt acaaatggga atacgatatg caacacaact catatcgccg ttggataacc
    28801 ctcgcgataa ttagttttag cggcggcgtt agttttgacc tggcttattt acgttatatt
    28861 tatcaaattc ccatggcgaa atttatggga ttcagcaata ccgagatagg tttaataatg
    28921 agtacctttg gtattgcggc tattattctt tatgccccca gcggcgttat tgccgataaa
    28981 ttttcacacc gcaaaatgat tacttccgcg atgatcatta ccggattact gggtctgtta
    29041 atggcaacgt atccaccgct gtgggtaatg ctctgtattc aggtcgcctt tgcgataacg
    29101 acgattttaa tgctgtggtc ggtgtcgatt aaagccgcct cgttgcttgg cgatcatagc
    29161 gagcaaggga aaattatggg ctggatggaa gggctgcgcg gcgtcggtgt aatgtcgctg
    29221 gcggtgttta ccatgtgggt cttttctcgc tttgcaccgg atgacagcgc cagcctgaaa
    29281 acagtcatta tcatctacag tgtggtttac atcttgttgg ggattctgtg ctggtttttt
    29341 gttagcgata acaacaacct gcgcagtgcc aataacgaag aaaaacagtc attccagctt
    29401 agcgacatcc ttgccgtttt gcgtatcagc accacctggt attgcagcat ggtgattttt
    29461 ggtgtcttca caatctacgc cattctgagt tactccacca actatctgac cgaaatgtac
    29521 ggcatgtcgc tggtggcagc gagctacatg gggattgtga tcaacaaaat cttccgcgcg
    29581 ctgtgcggcc cacttggagg cattatcacc acctacagta aagtgaaatc ccctacccgc
    29641 gtgattcaaa tcctttccat catcggtttg ctggcgttga cagccctgct cgtcacgaac
    29701 tctaacccgc aatcggtcgc gatggggatt ggcctgattt tactgctggg attcacctgt
    29761 tacgcctcac gcgggctgta ctgggcctgc cccggcgaag cgagaacacc gtcttacatt
    29821 atgggcacca cggtaggtat ttgttcggtg attggattcc tgccggatgt cttcgtttac
    29881 ccgattatcg gccactggca agacaccctg cccgctgcag aagcctaccg caatatgtgg
    29941 ctgatgggca tggcggcgct tgccatggtg atagtcttta cctttttgct gttccaaaaa
    30001 attcgtactg ctgatagcgc ccccgcaatg gctagcagca agtaagccag gaaaattaca
    30061 ggagaaaaat catgtcgaaa aaatacatca tagggattga tggcggaagt cagagcacaa
    30121 aagtggtgat atacgatctg gaaggtaatg tggtttgcga agggaaaggc ttattgcagc
    30181 cgatgcacac gccagatgcc gatactgcag aacatcctga cgacgattta tgggcatcat
    30241 tatgttttgc cggtcacgat ttgatgagtc agtttgccgg gaataaagaa gatattgtcg
    30301 gtattggtct gggatccatc cgttgctgcc gtgcgttatt gaaagccgat ggcacggccg
    30361 ttgattagct ggcaggatgc acgcgtaacg cgtccctacg aacacactaa tcctgacgtg
    30421 gcatatgtca cctctttttc gggttatctg acgcatcgct taaccggcga gtttaaagac
    30481 aatatcgcca actattttgg tcagtggccg gtggattata agagctgggc atggagcgaa
    30541 gatgctgcgg taatggagaa gtttaatatc ccccgtcata tactgtttga tgtgcaaatg
    30601 ccaggcactg ttcttgggca tatcacacca caagccgcgc tggcgacaca tttcccagct
    30661 gggctgccgg ttgtttgtac caccagtgat aaaccggtag aagctctggg ggccggatta
    30721 ctggatgatg aaacggcggc gatttcactc ggtacttata tcgcgctgat gatgaacggc
    30781 aaagccttgc cgaaagatcc ggtggcgtac tggccgatta tgtcttctat tccgcaaaca
    30841 ttgctgtatg aaggttacgg tattcgcaaa ggtatgtgga cggtgagctg gctgcgcgac
    30901 atgttaggcg agtcgttaat tcaggatgcc agggcgcagg atctttcacc ggaagattta
    30961 ctcaacaaga aagcctctag tgtgccacct ggctgtaatg ggctgatgac ggtgctggat
    31021 tggctgacca atccgtggga accgtacaaa cgcgggatta tgatcggctt tgattccagc
    31081 atggattacg catggatata tcgttcgata ctggaaagcg tggcgctgac gctgaagaac
    31141 aattacgaca atatgtgtaa tgaaatgaat cactttgcga agcatgtgat cattactggc
    31201 ggcggttcga acagcgatct gtttatgcag atttttgccg acgtgttcaa ccttccggca
    31261 cgacgtaacg ccattaacgg ttgtgcaagc ctgggggcag cgattaatac agcggtaggt
    31321 ctggggctat acccggatta cgcaacggct gtcgacaaaa tggttcgcgt gaaagatatc
    31381 tttatgcctg ttgagagcaa tgccaaacgc tacgacgcga tgaataaagg tattttcaaa
    31441 gacctaacca aacatactga tgtgatcctg aaaaaatcgt atgaagtgat gcatggggaa
    31501 ttggggaatg cggattcgat ccagagctgg tcgaatgcgt aagtgaggaa gaccgggcgg
    31561 gaaactgccc ggcctgaata tacctgaatg gttatccccg ctgacgcggg gaactcgaat
    31621 aactgaccaa cgtctggctc tctaaatccg gtttatcccc gctggcgcgg ggaacactct
    31681 aaacataacc tattattaat taatgatttt ttaagccagt cacaatctac caactttaca
    31741 ggatcacaca aacaacacat ccattatgtt aaagagcact taatccattg attaaaaagg
    31801 taaatattta aaataactct atacaaacta aaatctacca aacttcgccg caataatttt
    31861 cactccagcg aaaaattaac gccgcagaat ttgtagagaa tgataatgat tagtccggga
    31921 tgggcaagat ggatgagttt tcaggggcga caaataatac aaatgagtgg aattttttac
    31981 aagttccacc catttataa
//
LOCUS       DAJTYF010000037        29925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTYF010000037 DAJTYF010000000
VERSION     DAJTYF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.037"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            230..1678
                     /gene="uacT"
                     /locus_tag="OO590_001800"
     CDS             230..1678
                     /gene="uacT"
                     /locus_tag="OO590_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6637186.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1680..1805
                     /locus_tag="OO590_001801"
     CDS             1680..1805
                     /locus_tag="OO590_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637187.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1802..1873)
                     /locus_tag="OO590_001802"
     CDS             complement(1802..1873)
                     /locus_tag="OO590_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637188.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            1928..2476
                     /gene="idi"
                     /locus_tag="OO590_001803"
     CDS             1928..2476
                     /gene="idi"
                     /locus_tag="OO590_001803"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6637189.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            complement(2519..4036)
                     /gene="lysS"
                     /locus_tag="OO590_001804"
     CDS             complement(2519..4036)
                     /gene="lysS"
                     /locus_tag="OO590_001804"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6637190.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4046..5144)
                     /gene="prfB"
                     /locus_tag="OO590_001805"
     CDS             complement(join(4046..5068,5070..5144))
                     /gene="prfB"
                     /locus_tag="OO590_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6637191.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5235..6968)
                     /gene="recJ"
                     /locus_tag="OO590_001806"
     CDS             complement(5235..6968)
                     /gene="recJ"
                     /locus_tag="OO590_001806"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6637192.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(6974..7684)
                     /gene="dsbC"
                     /locus_tag="OO590_001807"
     CDS             complement(6974..7684)
                     /gene="dsbC"
                     /locus_tag="OO590_001807"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6637193.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(7709..8605)
                     /gene="xerD"
                     /locus_tag="OO590_001808"
     CDS             complement(7709..8605)
                     /gene="xerD"
                     /locus_tag="OO590_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6637194.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8717..9238
                     /gene="fldB"
                     /locus_tag="OO590_001809"
     CDS             8717..9238
                     /gene="fldB"
                     /locus_tag="OO590_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6637195.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9278..9685)
                     /locus_tag="OO590_001810"
     CDS             complement(9278..9685)
                     /locus_tag="OO590_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR6637196.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9666..9932)
                     /gene="sdhE"
                     /locus_tag="OO590_001811"
     CDS             complement(9666..9932)
                     /gene="sdhE"
                     /locus_tag="OO590_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6637197.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            10175..11155
                     /gene="ygfZ"
                     /locus_tag="OO590_001812"
     CDS             10175..11155
                     /gene="ygfZ"
                     /locus_tag="OO590_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6637198.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11351..12010)
                     /locus_tag="OO590_001813"
     CDS             complement(11351..12010)
                     /locus_tag="OO590_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6637199.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12174..12485)
                     /locus_tag="OO590_001814"
     CDS             complement(12174..12485)
                     /locus_tag="OO590_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR6637200.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            12530..13962
                     /gene="bglA"
                     /locus_tag="OO590_001815"
                     /pseudo
     CDS             12530..13962
                     /gene="bglA"
                     /locus_tag="OO590_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
     gene            complement(14019..14762)
                     /locus_tag="OO590_001816"
     CDS             complement(14019..14762)
                     /locus_tag="OO590_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6637201.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15029..17902)
                     /gene="gcvP"
                     /locus_tag="OO590_001817"
     CDS             complement(15029..17902)
                     /gene="gcvP"
                     /locus_tag="OO590_001817"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6637202.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18021..18410)
                     /gene="gcvH"
                     /locus_tag="OO590_001818"
     CDS             complement(18021..18410)
                     /gene="gcvH"
                     /locus_tag="OO590_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6637203.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18434..19528)
                     /gene="gcvT"
                     /locus_tag="OO590_001819"
     CDS             complement(18434..19528)
                     /gene="gcvT"
                     /locus_tag="OO590_001819"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6637204.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLIVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            19819..19902
                     /locus_tag="OO590_001820"
     CDS             19819..19902
                     /locus_tag="OO590_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637205.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            complement(19976..21178)
                     /gene="ubiI"
                     /locus_tag="OO590_001821"
                     /gene_synonym="visC"
     CDS             complement(19976..21178)
                     /gene="ubiI"
                     /locus_tag="OO590_001821"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6637206.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(21201..22379)
                     /gene="ubiH"
                     /locus_tag="OO590_001822"
                     /gene_synonym="visB"
     CDS             complement(21201..22379)
                     /gene="ubiH"
                     /locus_tag="OO590_001822"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6637207.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22376..23701)
                     /gene="pepP"
                     /locus_tag="OO590_001823"
     CDS             complement(22376..23701)
                     /gene="pepP"
                     /locus_tag="OO590_001823"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6637208.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23727..24305)
                     /gene="ygfB"
                     /locus_tag="OO590_001824"
     CDS             complement(23727..24305)
                     /gene="ygfB"
                     /locus_tag="OO590_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6637209.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24473..24802
                     /gene="zapA"
                     /locus_tag="OO590_001825"
     CDS             24473..24802
                     /gene="zapA"
                     /locus_tag="OO590_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6637210.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24844..25027
                     /gene="ssrS"
                     /locus_tag="OO590_001826"
     ncRNA           24844..25027
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO590_001826"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25048..25650
                     /locus_tag="OO590_001827"
     CDS             25048..25650
                     /locus_tag="OO590_001827"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6637211.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25747..25806)
                     /locus_tag="OO590_001828"
     CDS             complement(25747..25806)
                     /locus_tag="OO590_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6637212.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26139..26198)
                     /gene="ibsC"
                     /locus_tag="OO590_001829"
     CDS             complement(26139..26198)
                     /gene="ibsC"
                     /locus_tag="OO590_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6637213.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26427..27659)
                     /gene="serA"
                     /locus_tag="OO590_001830"
     CDS             complement(26427..27659)
                     /gene="serA"
                     /locus_tag="OO590_001830"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6637214.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(27915..28574)
                     /gene="rpiA"
                     /locus_tag="OO590_001831"
     CDS             complement(27915..28574)
                     /gene="rpiA"
                     /locus_tag="OO590_001831"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6637215.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            28957..29850
                     /gene="argP"
                     /locus_tag="OO590_001832"
     CDS             28957..29850
                     /gene="argP"
                     /locus_tag="OO590_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6637216.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
BASE COUNT         7107 a         8063 c         7502 g         7253 t
ORIGIN      
        1 catgccagaa tgattaatga aatatcaata tgagaaaatt acagataatt ccattgtcag
       61 gttctcttgt cattttcgtg gtttaagcac gattatgtga ttgcctccgc aattcctgtc
      121 tctaactccc cttcctcgca aaaactggca ctccacgagc atgtgtttag acagtttcat
      181 taacgtaaac ggttgctttt tattctggcg ggcgaaagga gaaacactga tgagcgccat
      241 agattcccaa cttccctcat cttctgggca agaccgccca actgatgagg ttgaccgcat
      301 attatcacca ggaaagctga tcatactcgg tctgcaacac gtccttgtca tgtacgcagg
      361 tgcagtcgct gttcctctta tgattggtga ccgactgggc ctctcaaaag atgctattgc
      421 gatgctcatt agctcggatc tcttttgctg cgggatcgtc acattattgc aatgtatcgg
      481 tatcggccgc tttatgggga tccgcctgcc ggtgattatg tcggtgacct ttgctgctgt
      541 aacaccaatg atagccattg ggatgaaccc ggatatcggc ctgctgggga tatttggtgc
      601 cactatcgcc gcgggtttta tcaccacatt attagcgcca cttattggtc gcttgatgcc
      661 tttattcccg ccactggtta ccggtgtggt tattacttct attgggctta gcatcattca
      721 ggtgggtatt gactgggccg ctggaggtaa agggaatccg caatatggta atcccgttta
      781 tttaggtatc tcctttgccg tcttaatttt tatcttgctc attactcgct atgcgaaagg
      841 atttatgtcc aacgtcgccg tattactggg gattgtattt ggctttttac tttcgtggat
      901 gatgaatgaa gtcaatttat ccgggctaca tgatgcctcg tggtttgcga ttgtcacccc
      961 gatgtcgttt ggtatgccga ttttcgatcc cgtttccatt ctgaccatga ctgccgtgtt
     1021 aatcatcgtg tttatcgagt cgatggggat gttcctggca ctgggtgaaa tagtcggtcg
     1081 taaactctct tcacacgata ttattcgcgg gctgcgtgtc gatggcgtag ggacaatgat
     1141 aggcggcacg tttaacagct tcccccacac gtcattttct caaaacgttg gcctggttag
     1201 cgtgatgcgc gttcatagcc gctgggtgtg tatttcttcg ggaattatat taatcctgtt
     1261 tggcatggtg ccaaaaatgg cggtgctggt agcctccatt ccgcaatttg tgctgggcgg
     1321 cgctggtcta gtgatgttcg gcatggtact ggcgacaggg attcgaattc tgtcgcgctg
     1381 taactacacc accaaccgtt acaacctcta tattgtggcg atcagtctcg gcgttggcat
     1441 gactccgacg ctctctcacg atttcttttc taagttaccg gccgtactgc aaccgctgct
     1501 acatagcggc attatgctcg caacccttag cgccgttgtg ctgaacgtct tctttaatgg
     1561 ctatcagcat catgctgacc tggtgaagga atccgtctct gataaagatt taaaagtcag
     1621 gacagtacgt atgtggcttc tgatgcgcaa gctgaagaaa aatgagcatg gagaataata
     1681 tgaatttttt aatgcgcgct atattcagtc tgttgttgct ttttactctc tctattcctg
     1741 tcatttctga ctgtgttgca atggccattg aaagtcgctt caaatatatg atgctacttt
     1801 tttaaatggt ttttacctgt cggcatccgc tcaaaacggg cggttgtcga taaacgctct
     1861 cttggttaat catttcactc ttcaattatc tataatgatg agtgatcaga attacatgtg
     1921 agaaattatg caaacggaac acgtcatttt attgaatgca cagggagttc ccacgggtac
     1981 gctggaaaag tatgccgcac acacggcaga caccctctta catctcgcgt tctccagttg
     2041 gctgtttaat gccaaggggc aattattagt tacccgccgc gcactgagca aaaaagcgtg
     2101 gcctggcgtg tggactaact cggtttgtgg gcacccacaa ctgggagaaa gcagcgaaga
     2161 cgcggtgatc cgccgttgcc gttatgagct tggcgtggaa attacgcctc ctgaatctat
     2221 ctatcctgac tttcgctacc gcgccaccga tccgagtggc attgtggaaa atgaagtgtg
     2281 tccggtattt gccgcacgca ccaccagtgc gttacagatc aacgatgatg aagtgatgga
     2341 ttatcaatgg tgtgatttag ctgatgtatt acgcggtatt gatgccacgc cgtgggcgtt
     2401 cagtccgtgg atggtgatgc aggcgacaaa tcgcgaagcc agaatacgat tatctgcatt
     2461 tacccagctt aaataaaaaa ccccgacatt tgccggggtt gtgagcataa cgtaatgctt
     2521 atttcaccgg acgcatcgcc gggaacagaa taacgtcgcg gatggtatgg ctgttggtga
     2581 acagcattac catacggtcg atgccaatcc ccagacctgc tgtcggcggt aagccatgtt
     2641 ccagtgcggt aacgtaatct tcatcgtaga acatcgcttc gtcgtcacct gcgtctttcg
     2701 cggcaacctg atccaggaag cgttgcgcct gatcttccgc gtcattcagc tcgctaaagc
     2761 cgttaccgat ttcacgacca ccgatgaaga actcaaagcg gtctgtgatt tccgggttaa
     2821 tgtcgttacg acgcgccagc ggagaaactt ctgccggata ttcagtaatg aaggtcggct
     2881 gaatcagatg tgcttctgcc acttcttcga agatctcggt aacgatacgg cccagacccc
     2941 agctcttctc aacgtggata ccgatagatt cagcaatcgc tttcgcagag tcgaagttat
     3001 ccagatccgc catgtcggtt tccgggcgat atttcttgat cgcttcacgc atggtcagtt
     3061 tttcgaacgg tttaccgaag tccagcgtca cgtcgccgta ggtcacttcc gtcttaccga
     3121 gaatatcctg tgccagagta cggaacagcg attcggtcag ctcgatcaga tctttgtaat
     3181 ctgcgtaagc catgtagagt tccatcatgg tgaactctgg gttatgacgt acggaaatac
     3241 cttcgttacg gaagttacgg ttgatttcga atacacgctc gaagccaccg accaccagac
     3301 gcttgaggta cagttccggc gcgatacgca ggtacatgtc gagatccagc gcgttatggt
     3361 gggtgataaa cggacgcgca gcggcaccgc ctgggatcac ctgcatcatc ggcgtttcaa
     3421 cttccataaa gccacggttc accatgaact ggcgaatacc agagaggatc tgcgagcgta
     3481 ctttaaaggt gttacgggat tcatcgttag agatgagatc cagataacgc tgacgatagc
     3541 gcgcttcctg atcctgcaag ccgtggaatt tatccggcag cggacgcagt gctttggtca
     3601 gcagacgcag ctcggtacag tggatagaca gttcgccggt tttggttttg aacagtttac
     3661 ctttcgcgcc gaggatgtcg ccgaggtccc attttttgaa ctgctcgttg taaacgcctt
     3721 ccggcagatc gtcacgggca acgtacagct gaatgcggcc acccacatcc tgcagggtaa
     3781 cgaaagacgc tttacccata atacggcggg tcatcatgcg gccagcaacg gcgacttcga
     3841 tgttcagcgc ttccagttct tcgttctctt taccgtcgaa ttctgcgtgc aattggtcag
     3901 aggtatgatc gcgacggaaa tcgttcggga aggcaattcc ctgctcacgc aggttcgcca
     3961 gcttctcacg acgcgttttc agttcattgt taagatcgac taccgcgtca gcgccctgtg
     4021 cgtgttgttc agacatgttg gttcctcata accctgcttt caaacttgct tcgataaatt
     4081 gatccaggct gccgtccagc acggcctgcg tgttgcgagt ttctaccccg gtgcgcagat
     4141 ctttaatgcg ggagtcatca aggacataag aacgaatctg gctgccccag ccgatgtcag
     4201 atttgttatc ttccatcgcc tgtttctcgg catttttctt ctgcatctcc agttcataaa
     4261 gcttcgcttt catctgcttc atggcctgat ctttgttctt gtgctgggaa cggtcgttct
     4321 ggcactgggt cacgatcccg gtcgggatgt gggtaatacg caccgcagat tcggtacggt
     4381 taacgtgctg accgcccgcg ccggacgcgc gataaacgtc aatgcgcaga tccgccgggt
     4441 tgatttcgat atcaatatca tcatcaactt ccggataaac aaacgcagag ctgaacgacg
     4501 tgtggcgacg accgccggag tcaaacgggc ttttacgcac caggcggtga acgcccgttt
     4561 ctgtacgcag ccagccgtaa gcgtaatcgc cggagatttt gatcgtcacg gatttaatac
     4621 ccgccacttc accttccgac tcttcgatga tttcagtttt gaaaccacgc gattctgccc
     4681 agcgcagata catacgctca agcatgctcg cccagtcctg tgcttccgta ccgccagacc
     4741 ccgcctgaat atcgaggtaa cagtcggcgc tgtcatattc gccagagaac atacggcgga
     4801 actcaagctg cgccagtttt tcttccaggg cgtcgagttc agcaacggct tcgttaaagg
     4861 tttcttcgtc gtcagcttct acagccagtt ccagcagacc agaaacatct tccagcccct
     4921 gtttcatttg gtcgagggtg tcgacaacgg cttcgaggga ggaacgctct ttacccagcg
     4981 cctgtgcgcg ttcgggttcg ttccagacat ccggctgttc cagctcggcg tttacttctt
     5041 ccagacgctc tttcttggcg tcgtagtcaa agataccccc taagaacgtc ggagcgttcc
     5101 gtgaggtcct gaatgcgatt atttaccgga ttaatttcaa acatggtctg atttctttta
     5161 ttgagctagt caaaatgcgg tgataagagc gggattgtac ccaatccacg ctctttttta
     5221 tagagaagat gacgctaaat tggccagata ttgtcgatga taatttgcag gctgcggttg
     5281 ccgcgaaact cgttgatatc gagcttataa gccagttgca cttcgcggac gccgttatcc
     5341 ggccagagag cggtatcgac attaaaagca ataccatcca gcagtggacc gccgccgacc
     5401 ggttcgacca tcaccttcaa atgacgttcg cccaccagcc gctgttgcag cagacggaaa
     5461 tgaccgtcaa acagcggctc cgggaacatc tgcccccacg ggccagcatc gcgcagcagc
     5521 tgcgccactt ccatggtcat ttcggccggg cttaacggac cgtctgacac cacttcgcct
     5581 tgcaatagcg aaggggccag ccactcagta accagttcgc caaaccgttg ttgaaagagt
     5641 tcgaatttat cctcttccag cgacaaaccc gccgccatcg catgaccgcc aaacttgagc
     5701 atcatgccgg ggtagagtgt gtctaatcgc tccagcgcat cacgcatatg cagcccctga
     5761 atggagcgac cggaaccttt cagcgtaccg tcacctgctg gcgcaaaggc gataaccgga
     5821 cggtgaaaac gctctttgat acgcgaagcc agaataccga caacgccctg atgccattcg
     5881 gggtgataca ttgccagccc gccgggtagc gtgtcacggc tgcgctccag tttctcgcac
     5941 agggtcaggg cttcaacctg cattccttgt tcgatctctt ttcgcgtctg gtttagcgca
     6001 tcgagttcat ttgccagcac gcgcgcttcg ccgatgttgt cgcacagcaa cagcgccaca
     6061 ccaacggaca tatcatccag tcgcccggca gcattgagac gcggtcccag cgcaaaacct
     6121 aaatcgctgg cggctagttt ttgtgcatcg cgatttgcca cttccagcag cgctttaatc
     6181 cctggacggc actttccggc acggatgcga ctcatcccct gccaggtcag aatgcgatta
     6241 ttagcgtcca gcggcacgac gtccgctact gtccccagcg ctaccagatc cagcagttct
     6301 gccaggttag gaattgcgat gccgcgctca tcaaaccagc cctgatcgcg caaaaaggtg
     6361 cgcagcgcca gcatcagata aaacgccaca cccacgcctg ccagtgattt cgacgggaaa
     6421 ttacagtcgc gcaagttagg gttaatgatc gcttccgctg cgggtaatgt gtcgcccggc
     6481 aaatggtgat cggtaacaat aaccgggatg cccaacgagc gagcgtgctc aactcccgca
     6541 tgggaggaaa taccgttatc caccgtgaca attaactgcg cgccacgggc atgggcctga
     6601 tcgaccactt ccgggcttaa gccgtaaccg tcttcgaaac ggtttggtac caggtagtcg
     6661 atattgctgc aaccaagcga gcgcatcgcc agcacgctta gagccgtgct ggtcgcgccg
     6721 tcggcgtcga aatcaccgac cacaataatc cgcgttcctt cgcgaaaagc gttgtaaagg
     6781 atctcaacgg ccttttcgac accgcttagt tgctgccagg gcaacatacc tttaacactg
     6841 cgttccagtt cttgcgcact gcgtactccc cggctggcgt ataaacggcg cagcaaggga
     6901 ggcaattcag cgggcaagtc tgccgtttca tcgacttcac ggcgacgaag ttgtatctgt
     6961 tgtttcacgc gaattattta ccgctggtca ttttttggtg ttcgtcgagg aattctttca
     7021 tgtctttcgg cggctggtaa cccggaacaa gtgtgccatt gctcagcaca actgccggag
     7081 taccgctaac gccaagctgg acgccaagtg cgtaatggtc ggcaatatcc acgtcgcagc
     7141 tggctggtgc gacgctttta cctgccatca catcatcaaa cgctttgttt ttatctttcg
     7201 cacaccagat agctttcatt tctttctctg catcgctgtc cagcccctgg cgcgggaaag
     7261 caagataacg cacggtgatc cccagcgcgt tatagtctgc catttgctca tgcagtttgt
     7321 ggcagtaacc acaggtaata tcagtaaaca cggtgatgac gtgtttttcc tgcggcgctt
     7381 tataaacgat catctctttt tcaagcgcat tcaactgctt taacagcatc ttattggtga
     7441 cattgaccgg agccgtgcca ctaacgtcat acattggccc ctgaatgata tgtttaccat
     7501 catcggtgat gtacaacaca ccgctgttag tcagaactgt cttcatgcca gctacgggcg
     7561 cgggctgaat atcgctgctt ttgatgccca ttttggctaa cgtttgttga attgccgcgt
     7621 catcagcctg agcaaagcct gaaaacgccg ctaacaaagt aaacaacata aaacctttct
     7681 tcataaatct tcccgttctt ttcagacatc acgcccgcgg gtgatgctgt tgatgaagtt
     7741 gccgcagacg ctcggtagcg acatgcgtat aaatttgcgt ggtggaaaga tcgctgtggc
     7801 ccagtagcat ctgcaccacg cgtaaatccg caccatgatt taataaatga gtggcaaaag
     7861 cgtgacgcaa cacatgcggt gacagctttt cgctgtcgat acccgccagc acggcataat
     7921 gtttaatgcg gtgccagaag gtctgtcgcg tcatctgctg cgcacgctgg ctgggaaaca
     7981 acacgtcaat tgacacacca ttcagcagcc acggacgccc atgttccaga taggtttcca
     8041 gccagtaaac cgcctcttcc cctaacggca ccaggcgctc tttgttgcct ttaccaatga
     8101 cccgcactac gccctgacgc aggctgatat cactcattgt cagcccgacc agttcagaga
     8161 cacgcagccc ggtagcatac aacacttcaa gcatggcttt atcgcgtagc tccagtggct
     8221 gatcaattaa tggcgcctgt aataaacgtt cgacctgcgc ttcgcttaaa tcttttggca
     8281 aacgctgggg caatttcggt gaagcgagat gcgcactggg atcgtcttca cgaaactttt
     8341 cgcgataaag atactggaac aatcggcgca ctgcactcag caaacgcgct gagctggtgg
     8401 ctttataccc gccctccagc cgttctgcca gtaatgcctg caaatcgtca ctttgcgccg
     8461 tcgccagcgt caacccgcgg tgatgcaacc actccaccat cattgacaga tcgcgacggt
     8521 aagcgttcaa cgtattttca gccagatttt tttccagcca cagagcatca agaaactgct
     8581 cgatgcgtgc cagatcctgt ttcacttgcg ccccttatgg tcactcattt gatccattat
     8641 gccttattgt gccgtgacta aagcgattct gatacactag ctgcaaaagc cacagcagaa
     8701 tcgagaagct tacgttatga atatgggtct tttttacggt tccagcacct gttacaccga
     8761 aatggcggca gaaaaaatcc gcgatattat cggcccagaa ctggtgacct tacataacct
     8821 caaggacgac tccccgaaat taatggagca gtacgatgtg ctcattctgg gtatcccgac
     8881 ctgggatttt ggtgaaatcc aagaagactg ggaagccgtc tgggatcagc tcgacgacct
     8941 gaaccttgaa ggtaaaatta ttgcgctgta tgggcttggc gatcaactgg gatacggcga
     9001 gtggttcctc gatgcgctcg gtatgctgca tgacaaactc tcgaccaaag gcgtgaagtt
     9061 cgtcggctac tggccaacgg aaggatatga atttaccagc ccgaaaccgg tgattgctga
     9121 cgggcaactg ttcgtgggtc tggcgctgga tgaaactaac cagtatgacc ttagcgacga
     9181 gcgtattcag agctggtgcg agcaaatcct caacgaaatg gcagagcatt acgcctgatc
     9241 tcactgacgg cttagcgcat atgctttgcc gtcatcttta tctttgcgtc tcttgttgca
     9301 acaaaatccg ccgtaaatcc cgccattcgg cttcgtccat gctgtcggct gccagccata
     9361 aatgttgccg tttaccgcca tcagaacgta aacgcagcat catgccgctc ttaatcatcc
     9421 acggtgcttt gacgatgctc cactcctgcc cttgccaacg caaacgcccg tccatcaaca
     9481 agcgaatttc cccctggcga gcattaatac accgctggct gcgaacgcaa tcaaatacca
     9541 ccagcgaaag taacaccatc cataacgggg tgtaactgag cggccagggc atgagtaaaa
     9601 taacagcggc aaccagccca tgaatcagca aggaaagcca ctgtgcgcgc caggagacgc
     9661 gcaaatcaga ttgccacagg accacgttcc cggttccgtg tctggatgag tcggaccatt
     9721 atttccagtt ctgcatcggc cggtttaccg tgattcatca gccagttaaa caggtccgga
     9781 tcgtcacatt ccagcagacg aataaagatg cgtttttcgt catcgcttaa gctgtcgtac
     9841 tcatgttcga aaaacggcat gattgaaata tcgagttcgc gcataccacg gcggcatgcc
     9901 caatgaatgc gggctttgtt gttaatgtcc atcttcttcc tgtctcacga aaatccagta
     9961 cccggctatt gtaacgtgtt tttcgacttc ttttacggga atatcagtaa acaccatcgc
    10021 gatcgcgaaa ttaattcaca acaattcaag ggcttcattt ttttggcagt cgcctcgcag
    10081 aaggcacaga tcgcgtagtg aaagcacttg cattgcctca tagctctttt accattagtc
    10141 attaatacgc cgttaagcaa ctcaggactc tattatggct tttacacctt ttcctccacg
    10201 tcagcctacg gcttctgccc gtttaccgct gacgctgatg acgcttgatg actgggcgct
    10261 tgccaccatt actggcgcgg acagcgaaaa atatatgcag ggtcaggtga cagcagatgt
    10321 cagccagatg gcagaagatc agcacctgct cgccgcccat tgcgacgcca aaggtaaaat
    10381 gtggagcaat ttacgtctgt tccgcgacgg cgatggcttt gcatggattg aacggcgcag
    10441 cgtgcgtgaa ccgcagctga ctgaactgaa aaaatatgcg gtattctcta aagtgaccat
    10501 cgcgccagac gacgagcgtg tgctgcttgg tgttgccggt tttcaggcgc gcgccgcgct
    10561 ggcaaatctc tttagcgaac tgccttcgaa agaaaaacag gtagtcaaag aaggcgcgac
    10621 cactttgcta tggtttgaac acccggcaga acgtttcctg atcgtaaccg atgaagctac
    10681 tgctaatatg ctgaccgata aactgcgcgg tgaagcggaa ctgaacaata gccaacagtg
    10741 gcttgcatta aacattgaag ctggtttccc ggtgattgat gccgccaaca gcgggcagtt
    10801 tatcccacag gcgaccaatc tccaggcgct gggcggtatc agctttaaga aaggctgtta
    10861 taccggacaa gagatggtgg cgcgagcaaa attccgtggt gccaacaaac gcgcgctctg
    10921 gttgctggca ggtagtgcca gccgactgcc ggaagctggt gaagacttag agctgaaaat
    10981 gggcgagaac tggcgtcgta ccggtacggt gctggctgcg gtaaaactgg aagatggtca
    11041 ggtcgtggta caggtcgtca tgaataacga tatggaaccg gacagcatct tccgcgtacg
    11101 cgacgatgcg aatacactgc atatcgagcc gctgccgtat tcgctcgaag agtaaatctc
    11161 tgtatcgcat caggcattta tcgcctgatg cgacgctgac gcgtcttatc atgtctggga
    11221 tttgatgcct ttatcacaac agggacgagg cccgtagatc ggataaggcg ttcacgccgc
    11281 atccggcggt tgcgctccga tgcctgatgc gacgctgacg cgtcttatca ggcctacaaa
    11341 ggcataccca ttacgcctgc ccaatataca aatagatcgc cagaaagtgg cacacactac
    11401 cgccgagcac gaagccgtgc cagatggcat ggttgtatgg aatgcgtttg cagacgtaga
    11461 aaatcacccc gagcgaataa accacgccgc ctaccgccag taaggtaacg ctgcccgccg
    11521 cgagcttaac tgccatttca taaattacca ccagcgacag ccagcccatc gccagataag
    11581 tcaccagaga taaaattttg aatcggtgcg cgatggtcag tttgaacaga atccccagca
    11641 atgctaggct ccagataaca atcatcaacc cgcgcgctaa cggagaatcc agccccacca
    11701 gcaaaaacgg cgtgtaggtt ccggcaatca acaggtaaat agcgcaatgg tcaaatttct
    11761 tcagccacat ttttgcccgt tgatgaggaa tggcgtgata gagcgtcgaa gcgaggaaca
    11821 gcaggatcat actgccgcca tagaggctgt aactggttat cgctgtggcg ctggcattga
    11881 gatccaccgc ctgaaccagt agcaacacca gcccaacgat accaaacacc agcccaatgc
    11941 cgtgactgac gctgttggca atttcctctg ccagcgaata tccctgctta atgaggggct
    12001 tctgaaccat aacttactcc agagaaacgt acacgcacat gtatacctct ccagcgtaac
    12061 tgagaatagt tccagtgaac acctgttagc taaaataaat tttgatttga gactaatctc
    12121 ctaaaaatca tgaaattaaa tgcgaaattt caactaacag gcgtcagttc aatttaaaga
    12181 catttaaatt caatcatata aaattgtgtc tgaccgggat agatgtcagc aatgaccttt
    12241 ttcagttcag gcagggtcat attttcctgc tctgcatgtt tttccgtcag tgtatccagc
    12301 gttacggttg aggttgcggt gacttcaatc gtgcaaaaat aaccgtcatc ttcaaaacgt
    12361 ccgacacgaa gcacatcacc cgttttgaag tgcgattcag actcgtcgcg gatggtgatg
    12421 gttttacgcc cagccagaat gtcatcctgg aaacgttgaa aaaaagtgat gtcgtttggc
    12481 tgcatggtac tatttcctgt aagaattgac tgatctggag cctatgatag tgaaaaaact
    12541 caccttaccg aaagatttct tatggggtgg cgccgttgcc gctcatcagg tcgaaggcgg
    12601 ctggaacaaa ggcggcaaag gaccgagcat ttgtgacgtt ctgaccggtg gcgcacacgg
    12661 cgtgccgcgc gaaatcacca aagaagtctt gccaggaaaa tactatccaa accatgaagc
    12721 cgttgatttt tatggtcact acaaggaaga catcaagcta tttgccgaaa tgggcttcaa
    12781 atgttttcgg acttccatcg cctggacgcg tatttttcca aaaggtgatg aagctcagcc
    12841 aaacgaagaa gggctgaagt tttacgactc tctgttcgat gaactgctga aatacaacat
    12901 cgaaccggtg ataaccctct cccactttga aatgccgctg catctggtgc agcaatacgg
    12961 tagctggacc aaccgtaaat tcgttgattt ctttgtccgt ttcgcggaag tggtattcga
    13021 acgctacaag cataaagtca aatactggat gaccttcaac gaaataaaca accagcgtaa
    13081 ctggcgtgcg ccgctgtttg gttactgctg ctccggcgtg gtgtataccg agcatgacaa
    13141 cccggaagag acgatgtacc aggtgctgca tcaccagttt gtcgccagcg ccctggcagt
    13201 gaaagccgcg cgtcgcatta acccggagat gaaagtcggc tgtatgctgg cgatggtgcc
    13261 gctctatccc tactcatgta acccggacga tgtgatgttc gctcaggagt cgatgcgcga
    13321 acgctacgtc tttaccgatg tgcagctatg cggctattac ccgtcctatg tattgaacga
    13381 gtgggaacgt cgcggattta atatcaaaat ggaagacggc gatctcgatg tactgcgcga
    13441 aggcacctgc gattatcttg gtttcagcta ttacatgacc aacgcagtga aggccgaagg
    13501 cggcaccggc gatgcgatct ctggttttga aggcagcgta ccgaacccgt atgttaaagc
    13561 gtctgactgg ggctgcagat tgatccagta ggtctgcgct atgccctttg cgaactgtat
    13621 gagcgttatc agaggccgct gtttattgtc gaaaacggtt ttggcgctta cgacaaagtg
    13681 gaagaagatg gcagcatcaa cgacgactac cgcattgact acctgcgcgc ccatattgaa
    13741 gagatgaaaa aagcagtgac ttacgatggt gtggatctga tgggctacac accgtggggc
    13801 tgcatcgact gcgtgtcatt cactaccggg cagtacagca aacgctacgg ctttatctat
    13861 gtgaataaac atgacgacgg tactggcgat atgtcgcgtt cacgtaagaa gagctttaac
    13921 tggtacaaag aggtgattgc cagcaacggc gagaagcttt aagtcgatga agtaccggat
    13981 gcaatacttg ttgcatccgg tcagacaact tccctgtttt atttcccgcc cgccaaatcg
    14041 ataaaacttc ccgtgacgta agaggcttta tcacttagta gccagacaat ggcctgcgcg
    14101 acctcttctg cctgtccacc acgctgcatg gggatgttcg acttaacgcg atcgacgcgt
    14161 ccaggctcgc cgccgctggc gtgcatttcg gtataaataa accctggccg cacgcagtta
    14221 acgcggatcc cctgcgcggc gacttccagc gatcgtccgg tggttaacgt atcaatcgcc
    14281 cctttcgatg ccgcgtaatc aacatattcc cctggcgaac ccaaccgtga ggccaccgaa
    14341 gagacattga cgatagcgcc gccactgcca ccatttttaa gcgccatgcg ttttaccgcc
    14401 tcgcggcagc agagaaaata tcccgtcacg ttggtggaaa gtactcggtt gattcgctct
    14461 gcggtaaggt tttcaacggt gcactgggta aacaagatcc cggcgttatt gaccagcgct
    14521 gctagcggtt catcgtgctg atcgattgct gtaaacatcg caacgacctg gttttcgtcg
    14581 ctgatatccg cctggagcac gaatgctttg ccaccggctt gcattattaa gttcatcact
    14641 tcctgcgccg cgtggaggtt ttgctgataa ttaaccgcca ccgtataccc ttcttgcgcc
    14701 aacagtaatg cagttgcccg cccgatgccg cgactgccac cggtcacaag tgctatagcc
    14761 atttgttttt ccgagaaaaa ttaattcgtt gacgtatctt tgagattgct aacaaactgg
    14821 ctcaatcttc aatgttggca cgcttttccc cctcgccctt tcagggagat ggtcggggtg
    14881 agggtaaata ttcgcgccag tgccggcctg ttcccctcac cctaaccctc tccccaaagg
    14941 ggcgagggga ctgtctgagc acttttgtac tttgtcatct gactaaaaag gcgccgaagc
    15001 gcctttagaa aatactcgaa tcagtgaatt actggtattc gctaatcggt acgcaggagc
    15061 agaacaggtt acggtcgccg taaacatcat ccagacgttt caccgtcggc cagtatttgt
    15121 ctgccacgcc tgccgggaat accgcaactt cacggctgta cggatgcgcc cactcggcga
    15181 ccagttcgct ctgaatgtgc ggcgcgttca ccagcgggtt atcttccagc ggccagacac
    15241 cggctttcac ctggtcaatt tctgcgcgga tagccagcat cgcgtcgata aagcgatcca
    15301 gttccacttt gctttcagat tcagtcggtt caaccatcag cgtacccgcc accgggaacg
    15361 acatcgtcgg cgcgtggaaa ccgtagtcga tcaggcgctt ggcaatatcc agctcgctga
    15421 tgccgatttc ttctttcagc gggcgaatat cgagaataca ttcgtgcgcc acgcgaccgt
    15481 cgcgaccggt atacagcacc gggaaggcat cctgcaggcg gctggcaata tagttggcgt
    15541 tgaggattgc cacctggctt gcttttttca gcccttctgc gcccatcatg cggatgtaca
    15601 tccagctgat tggcaggata gaggcgctac cgaacggtgc cgcagaaact gcgccctgac
    15661 gggttaacat gccttcgatt tgcaccacgc tatgacccgg tacaaacggt gccagatgcg
    15721 ctttcacacc gatcggtccc atacccggac caccaccgcc gtgcggaatg cagaaagttt
    15781 tatgcaggtt gaggtgcgaa acgtccgcgc caataaagcc cggcgaggtg atgccaacct
    15841 gggcgttcat gttcgcgcca tcaaggtaaa cctgaccgcc gaactgatgc acgacttcac
    15901 acacttcacg gatcgtttct tcatacacgc cgtgggtaga aggataagtc accatgatac
    15961 aggagaggtt atcgcccgcc tgttccgctt tcgcgcgcag atcagtcaga tcgatgttgc
    16021 cgtttttatc acacgccaca accaccacct gcattcctgc catatgtgca gaagcggggt
    16081 tagttccgtg cgcagaagcc gggatcaggc agatatcgcg atgcccttcg ttgcggcttt
    16141 catgataatg acgaatcgcc agcaggcccg cgtattcgcc ctgtgcgccg gagttcggct
    16201 gcatacaaac ggcgtcgtaa ccggtcagtt tcaccagcca gtcagccagc tgcgcaatca
    16261 tctgctgata accttcagcc tgttccggcg ggcaaaacgg gtgcagttcg gcaaattccg
    16321 tccaagtgat tgggatcatc tcggcggcgg cgttcagttt catggtgcag gaacccagcg
    16381 ggatcatcgc ctgattcagc gccagatctt tacgctccag cgagtgcata tagcgcatca
    16441 tttcggtttc gctgtgatag cgattaaaca ccggatgggt gaggatttcg tcgtcgcgca
    16501 gcatcgcagg ctggatagag cggctgtcgt gagccacgtc tttgtccagc gtgtcgatct
    16561 ccagcccgtg gttatcgccc agcagcacac tgaaaagctg cattacgttt tcacgcgtgg
    16621 tggtttcatc aagggtgatc cccacggcgt tcaggatatc gctacgcagg ttgatttcag
    16681 ccgcttcggc acgcgtcagt acgcccgctt tgtcggctac ttccacacac aaggtgtcga
    16741 aatagtgcgc atggcgcagt ttcagacctt tttgttgcag gcccgccgcc agaatatcgg
    16801 tcagacggtg aatgcggtta gcgatacgtt tcaggccaac cgggccgtga taaacggcat
    16861 acaggctggc gatgtttgcc agcagtacct gggaagtaca aatgttggag ttcgctttct
    16921 cacgacgaat atgttgctcg cgagtctgca tcgccatgcg cagcgcggta ttgccagctg
    16981 catctttcga tacaccgata atacggcccg gcattgagcg tttgtattca tctttcgccg
    17041 caaagaatgc cgcgtgtggg ccaccgtagc ccatcggcac gccgaagcgt tgcgccgaac
    17101 caaaaacaat atccgcgccc tgtttacccg gcgcagttaa cagcaccagc gccataatat
    17161 cggcggcaac gctgaccaca attttgcgtg atttcagttc gctaataagc gcagtgtagt
    17221 cgtgaatttc accggtagtg cctacctgct gtaacagcac gccgaagacg tcctgatggt
    17281 cgagcacttt ttgcgcgtca tcgacaatca cttcaaaacc aaaggtttcg gcacgagtac
    17341 ggaccacatc cagtgtttgc ggatgcacat tggaagccac gaagaagcgg ttggcatttt
    17401 tcagtttgct gacgcgtttc gccatcgcca ttgcttcggc ggcagcggtg gcctcgtcca
    17461 gaagagaagc agaggccata tccagtccag tcaaatccag cgttacctgc tggaagttga
    17521 gcagtgcttc aaggcggccc tgggagactt caggttgata cggagtgtac gcggtatacc
    17581 agcccggatt ttccagcatg ttacgcagga taaccggcgg tagctgcacg gcggtgtaac
    17641 ccatgccgat gtaagacgtg aagcgtttat tgcgactggc aatagccttg agttctgcca
    17701 gtgcggcgta ttcggtcgcc ggtgcgccaa cctgcggtgg tgtcgcaagc tgaatatctt
    17761 tcggcacaat ctggccggtc agcgcgttta acgattgtgc accaacggca ttcagcattt
    17821 cttgctgttg cgcggcgtcc ggtccgatat ggcgttcaat aaaagcgccg ctgttttcaa
    17881 gctggcttaa cgtctgtgtc atgagcgatg gttcctgaaa cgtgcagtga attgtgaacc
    17941 tctctcctta cgaagagagt gagggtgagg cataaatttt cctcaccctg atcctctccc
    18001 gcagaagagg aataaagccg ttactcatct tctaacaatg cttcgtatgc ggtcgcatcc
    18061 agcagtgatt ccagttcgct ttcatcgcta gctttgattt taaagatcca gccgcctgca
    18121 tacggttcgc tgttcaccag ttccggggaa tcgctcagtg cgtcgtttac cgccacgatt
    18181 tcaccgctta ctggcgcata aatgtctgac gccgctttta ccgattcggc aaccgcgcag
    18241 tcatcgcccg cgctaaccgt tgcacccact tccggcaggt caacaaacac catatcgcct
    18301 aacagctcct gagcatgttc ggtaatacca acggtgtaag tgccgtcggc ttctttacgc
    18361 agccattcgt gttctttgct gtatttcagt tctgctggta cgttgctcat caatcaatct
    18421 ccaaaaaagt aaatcacgcg acggctttgc cgttacgcac aaaaacaggt tttgtcactt
    18481 taaccggcat ttcacggttg cgaatttgca caatcgccgt ttcgccaata ccttccggca
    18541 cgcgcgccag cgcaatgctg taacccagcg tcggggagaa agtaccgctg gtgataatgc
    18601 cttcatgctg gttgccctgc gcatcggtaa agcgtaccgg cagttcatta cgcagcacgc
    18661 ctttttcggt catcaccaga ccaaccagtt tttctgtacc atgctcacgc tgcacttcca
    18721 gggcttcacg accgataaag tcacgatctg tcggttccca ggcgatagtc cagcccatgt
    18781 tggcggctaa aggagaaata gtttcgtcca tctcctgacc ataaagattc atgcccgctt
    18841 ccagacgcag cgtgtcacgc gcccccagac cgcagggctt aacgcccgct tccaccagcg
    18901 cacgccagaa atcggccgct ttttcattgg gcagcgcaat ttcatagccc gcttcaccgg
    18961 tataaccagt ggtggcaata aacagatcgc ccacctgcac gccaaagaac ggtttcatcc
    19021 cttccaccgc ctgacgctga gcatcattaa acagtgtggc agcttttgcc tgagcattcg
    19081 gcccttgcac ggcaatcatg gaaaggtcat cacgaacggt aatttcgatg ccgaaaggtt
    19141 cagcgtgttg ggtaatccag gagaggtctt tttcgcgggt ggcggagtta acaatgaggc
    19201 ggaagaaatc ttcagtaaag tagtagacga tgagatcatc tatcacaccg ccagaggcat
    19261 tcaacatccc cgagtaaagg gctttgccgc ttttggtgag cttcgccaca tcgttcgcca
    19321 gcagataacg cagaaactcc cgggtgcggc tgccgcggag atcgacgatg gtcatatgcg
    19381 acacatcaaa cattccggca tcggtacgta ccgcatgatg ttcgtcgatt tgcgaaccgt
    19441 aatgcagcgg catcatccag ccgtggaaat ccaccatgcg agcgccgcaa agcgtgtgtt
    19501 gttcgtacaa aggagtctgt tgtgccatct tgtcctcatt gaataagcgg ggctgacaac
    19561 tttttcatgg tgaaattatc accacaaaac ccagcatcgg agccactccc ggtccccaac
    19621 gcaatcgttc tcttttgcct gaacttacca ccgaaacaga ctgttaacca taaggtaaaa
    19681 ttgatcatca cattagctta tggttaaaaa atgcaaaaat cgcgacagaa taaaaaacca
    19741 aaaaatacac caatttctat acaaagatga tgtgatgaga aagtcaattt gaataagaca
    19801 atattaagag ctaaaaaaat gtcaaaaaac actaaatcaa aaaatcatgg cattagaaaa
    19861 tataatgcga aaacggaggt gaaattagtt tatttcaaat gaggaaaatc tcccggcgaa
    19921 aaaaccggga gatgaaagtg tgatgggtat caaataaaca acagaggaga aatttttaac
    19981 gcagccattc agacaaatcg tttaatccca ttgcctggcg gataagttgc ggcttaacgc
    20041 caggaagcgt gtcggccagt ttcaaaccaa tatcacgcag cagttttttc gccggattag
    20101 cgccggaaaa gagatcgcga aaaccctgca taccagccag catcagcgcc gcgctgtgct
    20161 tgcggctacg ttcatagcgg cgcaggtaaa tgtactgccc gatgtcttta ccctgacgat
    20221 gcaaccgttt cagttcagca atcagctctg cggcgtccat aaatccgaga ttaacgccct
    20281 gccccgccag tgggtgaata gtatgcgcgg cgtcgcccac cagcgccaga cggtgtgcgg
    20341 caaactggcg cgcataacgc cccgtcagcg ggaacacctg acgcgcgctc tcaaccttgc
    20401 ataagccaag gcgattatca aaagcgatat ttaacgcgcg attaaattcg tcttcacttg
    20461 cctgctgcat ccgctgcgct tcctctggcg acagtgacca gacaatcgag caaagatgcg
    20521 gatcgctaag cggtaaaaag gccagaatgc cttcgccatg gaaaacctgc cgcgccaccg
    20581 catcatgcgg ttcttccgtg cgaatggtcg ctaccagcgc gtgatgctga taatcccaga
    20641 aagtcagcgg aatatctgct ttgttgcgca accaggaatt agcaccgtcc gcgccaatca
    20701 ccagacgcgc cgttaacatg ctgccatctt tcagcgtcag gaaggtttca ttttctcccc
    20761 aggcgacctg ctgtaattct gtgggggcca acagagtaat atctgacgac tgctgcgctt
    20821 tgttccacag cgcgtagtga atcactgagt tttcaacgat gtgcccaaga tggctatagc
    20881 ccatgctttg atcgtcaaac gagatgtgac caaaactgtc tttgtcccac acttccatgc
    20941 cgtgatagca actggcccta cgagcgagaa tgtcctgcca gacgccaaga cgggtgagta
    21001 atttttcgct ggcggcattg atagccgaaa cgcgcagttg tggtagtgca tctaccgcca
    21061 gaggttccgg tacgcgctgc tccagtacgg caacgcgtaa gccgctcccc tgtaagccgc
    21121 aggcaaccgc cagccccacc atgccgccgc caacaatggc tacatcaaca ctttgcattg
    21181 tttattcctt aaaaccgcct tcaacgcgcc acccaaccga gggtgcgctg cgccagcaca
    21241 tcgcgtgccg gggtgaataa ttccatcgtc atcagtccga tgttgcgccc gacaaccagc
    21301 ggtgcccaac ggttggcaaa aagatgtacc aggctgtccg tgacgccgat ggttgcttcg
    21361 cgatcgctct gtcgacgctg ctgatagcgg cacaatatgc cgtaatcacc gatgtcttct
    21421 ccgcgctcat gcgcctgagt cagggtttcc gcaaggctca tgacatctcg cataccgagg
    21481 ttaaaccctt gcccggcaat cgggtgcaga gtttgcgccg cattgcccac cagcacagtg
    21541 cgatgggtaa tcggtctggc ggcgcgggtt aatgccagcg gataagcact gcgtttaccg
    21601 gcgtgggtaa tttgccccag tcgccagcca aaggcagact ggagttcacg gcaaaacttc
    21661 tcgtcgctcc acgacagcac ctcttcgcgc cgttccagtg gatgacacca gaccagcgaa
    21721 cagcgtccgt cagacatcgg caacatcgcc agcgggccat gttgcgtgaa gcgttcaaaa
    21781 gcgcggcctt cgtgcgcaac agaagtagca acgttggcaa tcactgccag ttgttcgtaa
    21841 ggctcctgct gccaatcaac gccgcacgcg gtggctaacg ctgaatgggt gccatcagcg
    21901 gctatcagca cgcggcccgt cagcatctcg ccgctctcca gcgtcacttc aacgtgactc
    21961 tgagtacggg caacgttagc tacgcgatcg gggcaatgca gcattacgcc aggtgctttt
    22021 cgcagcaatg caaacagccg ttgcccaaca ttatgcaatt cgacaacctg ccccagcgcc
    22081 gccagttggt aatcttctgc ggcgagggtg acgaatccag catgaccacg atcgctgaca
    22141 tgcacggtag tgatggcggt tgcgcaatca gccagagatt gccagacgcc gatgcgcgcc
    22201 agttgctgac aggtacccgc cgccagcgct atcgctcgtc catcaaagcc cggatgagca
    22261 tgtgactctg gcgcagtcgc ttcaatcaaa tgtaccggca gcgccccgtg acttaaccgg
    22321 gaaatagcca gcgccagcgt cgcgcccgcc atgccgccac cgacgatgat tacgctcatt
    22381 gctttctcgc agcagccatc aacgcttcga tttcttccgg ctttttcacc acgctggcgg
    22441 tgaggttttc gttgccggtt tcggtaatca caatgtcgtc ttcaatacga atgccgatac
    22501 cgcgatattg ttctggcaca tccgcatccg gcgcgatata cagaccaggc tctacggtca
    22561 gtaccatccc cggttccaga atgcgcgagc gatcctgacc ataaacgcca acgtcatgga
    22621 catccagtcc taaccagtgg ctaaggccat gcataaagaa aggacgatgg gcgttctgag
    22681 cgatcagttc atcaacatta cctttcagga tgccgagttt taccaggccg ctaaccatga
    22741 tgcgcaccac ttcaccagtg acttccagaa tggaagttcc cggacgatac aggcgcaggc
    22801 tggtttcgag agactccagc acaatgtcgt agatttcacg ctgggcctgg gtgaatttgc
    22861 cgttgaccgg gaaggtgcgg gtaatatcgc ccgcgtaacc tttgtattca caacccgcgt
    22921 caatcaacac caggtcaccg tcgcgcagtt cactctcgtt ttcggtgtag tgcagaatgc
    22981 agccgttttc accgctgcct acaatggtgt tataggaagg atagcgcgca ccgtggcggt
    23041 taaattcgtg gtgaatttcg ccttccagat ggtactcgaa cattcccgga cggcattttt
    23101 ccatcgcccg tgtatgtgcc atggcggtga tttctcccgc gcggcggagt acggcaatct
    23161 cttctggcga tttgaacagg cgcatttcat gaacaacagg acgccagtcg atcatcgttg
    23221 ccggtgcggt gagattttgc cgcgaacctt tacgcagttt ttccagcgca ctgttcacga
    23281 tttcatcagc atatgcatat tcgccctggg catggtaaac cacatccagg ccgttaagta
    23341 gttgataaag ttgctgattg atttcgctga atgccagtgc gcggtcaacg cccagtttct
    23401 ctggcgcggc atcctggcct aagcgacggc caaaccagat ctccgccgtc aggtcgcgaa
    23461 cgcggttaaa cagaacgctg tggttatgag tgtcatcgct tttaatcagc accagcaccg
    23521 cttccggttc gttaaagccg gtgaagtacc agaagtcact gttctgacga taggggtatt
    23581 cgctgtcggc gctacgtgtt acttctggtg cagcaaaaat cagcgcggcg ctgccgggtt
    23641 gcatttgctc caccagggcc tgacggcgac gctgaaactc ttgccgggat atctcactca
    23701 taacactctc cttacgtttt ttgtttttag tgtagagtcg gtttttgtac ttctggcgcg
    23761 gtcggttgcg gatgagtaaa ggtgtcgtgg cataacagcg cggcaacacg aacgtattcg
    23821 atgatctctt caagcgacat ttcaagctct tcctgatctt cgtcttcgtc gtagcccagc
    23881 tgcgcgatgt tacgcagatc gtcgatggct tcaccggttt cgccggtcac tttgtccagc
    23941 ttcggttgcg taacgccaag accaagcagg aagtgattga cccaacctgc cagtgcatca
    24001 gcccgatcga aaacgctgac atcatcgcca tcaggcagat aaagctgaaa aaggaagccg
    24061 tcatcctgca gggcatcgct ggtggcagag tgcattttac gcagtgcctg tgccagctca
    24121 tgaccgaaag ccatgccttc gttcgtcagg tcgtgaagta gcggcagcca tgagctgtca
    24181 tcgttaccgc cacatatcat cccactgatt aaaccatgca tctcggcagg ggtcagaccc
    24241 gtcccttgtt ggttcagata ctggttcatt tcgttgtaac caggcatttc gttctgtata
    24301 gacataagca ttcgtcgtca aagggaggaa tattcatgat atgctaccac tttgggccct
    24361 ggtggaccag aaaagggctt gtctcttctc atcagggtag ctatagcgtc gccccttcgc
    24421 agaccatggg tctaaagacg aaggcagcgc agtcaatcag caggaaggtg gcatgtctgc
    24481 acaacccgtc gatatccaaa tttttggccg ttcactgcgt gtgaactgcc cgcctgacca
    24541 aagggatgcg ttgaatcagg cagcggacga tctgaaccaa cggttgcaag atctgaaaga
    24601 acgcactaga gtcacaaata ctgaacagtt ggtcttcatt gccgcattga atatcagcta
    24661 tgagttagcg caagaaaaag caaagactcg tgactacgcg gcaagtatgg aacagcgtat
    24721 tcggatgctg cagcagacca tagaacaagc gttacttgaa caaggtcgca tcaccgaaaa
    24781 aactaaccaa aactttgaat gacacttttc ggtttactgt ggtagagtaa ccgtgaagac
    24841 aaaatttctc tgagatgttc gcaagcgggc cagtcccctg agccgatatt tcataccaca
    24901 agaatgtggc gctccgcggt tggtgagcat gctcggttcg tccgagaagc cttaaaactg
    24961 cgacgacaca ttcaccttga accaagggtt caagggttac agcctgcggc ggcatctcgg
    25021 agattccctt cttatctggc accagccatg acgcaactac cagagctccc actgacatta
    25081 tcccgacaag aaatccgcaa aatgattcgg caacgtcgtc gtgcgttaac gccggaacaa
    25141 cagctggaaa tgggtcaaca agccgctacc cggatgatga cttatccccc ggtggtgatg
    25201 gcgcatacgg tcgctgtatt cctctctttt gatggtgaac tcgacaccca gccgctaata
    25261 gaacaactct ggcgcgccgg taagcgcgta tatcttccag ttttgcatcc ctttagtgcc
    25321 ggtaatttgc tgttcctgaa ttaccatccg caaagcgaac tggtgatgaa caggttgaag
    25381 atccatgagc caaaactgga tgtgcgtgac gtgctacccc tttcccgatt agacgtgctg
    25441 atcacaccgc tggtcgcctt tgatgagtac ggtcagcgcc tgggaatggg cggtggtttt
    25501 tatgatcgga ccttacaaaa ctggcagcac tataaaacgc aaccggtggg ttatgcgcat
    25561 gattgtcagt tggtggaaaa actccccgtt gaagagtggg atatccctct tcctgcggtg
    25621 gttacaccgt cgaaagtctg ggagtggtaa gggcgataca cctgcatcgc cctgattgac
    25681 atcgttgatt ctttaaccta atttagtgag taagggtaag ggaggattgc tcctcccctg
    25741 tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga tggcaagtcg
    25801 catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt tacagcccca
    25861 tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc tacctttaaa
    25921 tcttttcttc gagcgtgctc gaaaaacccc atgtaaaaca gagataattt ttgggaaaaa
    25981 ggatctatta cggcagtaaa cgtgggcgat gaagtggcta tcaaaaagtt cggttcaggg
    26041 cgatgcaccc cgcatcgccc tgattgacat cgttgattct ttgacctaat ttagtgagta
    26101 agggtaaggg aggatgactc ctcccttgag actgactgtt aataagctcc gaaacttatc
    26161 agtaacagta caatcagtat gatgacaagt cgcatcataa cccttctcct ttaagccctc
    26221 gcttcggtga gggctttacc gttacagccc catgctgtcc cgccatcgta aatccccatt
    26281 aaataaacac aacgcattga tctgactttg atttattttc tggagcagac tcgcaaagta
    26341 gaatgcgcaa cgcggcaacg gtgtggagaa gggataaaaa aacgggcaag tcagtgacct
    26401 gcccgttaat tttcagagaa ggggaattag tacagcagac gggcgcgaat ggtacccgga
    26461 atagctttca ttgcctgcag cgctttttcg gcaacgtctt cgtcagcttc aatatcaata
    26521 actacataac ccatctgggc ggaagtttgt agatattgcg cggcgatgtt gacgccctgc
    26581 tcggcgaaga ttttgttcag cgcagttagc acgcccggac ggttttcgtg gatgtgcatc
    26641 agacgacgac caccatgcag tggcagcgag acttccggga agttcaccgc agagagcgtt
    26701 gagccattgt cagaatactt gatcaattta cccgcaactt ccaagccgat attctcctgc
    26761 gcttcctgag tcgaaccgcc aatgtgtggc gtcagaagga cgttgtcgaa ttcacacagt
    26821 ggagaggtaa atggatcgct attggtcgcc ggttccgtcg ggaatacgtc gattgccgcc
    26881 cccgccagat gtttgctcgc cagcgcatca cacagcgccg gaatatccac cacagtaccg
    26941 cgcgaggcat taatcagcag cgagccgggc ttcattagcg aaatctcttt cgcgcccatc
    27001 atatttttgg tggacggatt ctctggtaca tgcagactca ccacatcgct catattcagc
    27061 aggtcagaaa gatgctgtac ctgagtggcg ttgcccagcg gcagtttgtt ttcaatatca
    27121 taaaagtaaa catacattcc cagcgattca gccagaatgc ccaattgcgt accaatatga
    27181 ccgtagccga tgatacccag ctttttgccg cgcgcttcaa aagaacccgc cgccagtttg
    27241 ttccacacgc cacggtgcgc tttagcattg gcttccggca cgccgcgcaa tagcagcagc
    27301 agttcgccaa tcaccagctc cgcaacagag cgcgtatttg agaacggtgc gttaaatacc
    27361 gggatcccgc gctttgccgc cgcatccaga tcaacctggt ttgttccgat acagaaacag
    27421 ccaatagcga ccagtttttc tgcggcgttg atcacgtctt cagtcagatg ggtacgggat
    27481 cgcaggccga tgaagtgggc atcgcggatg gattctttta attgttcatc atccagcgcg
    27541 cctttgtgaa attcgatgtt ggtgtaacca gctgcacgaa ggctttccag cgccttttgg
    27601 tgcacgcctt ctaccagcag aaacttaatc ttgtctttct ccagcgatac ctttgccatt
    27661 tacccaatcc tgtcttttga aatgttgtgt gcggatttgc atccgccttt caacatatca
    27721 aaaaataata ttgcggcaat atgaacgttt gcgtcgcgat gttgaggaaa tatcacgcaa
    27781 aggtaattct tggatgaata tgctggttta gaggatttat ttagagcaat cgacaattgc
    27841 ctggtaaaag cgtgacacat gtcaccaaat ttaatgaaga gaattttttt aacgggggag
    27901 gttcccccgt cagatcattt cacaatggtt ttgacaccgt caggtgtgcc aatcagcgca
    27961 acgtccgcgc cacggttagc aaacaagcca acagtcacca cgccaggaat cgcatttatg
    28021 gcgttttcca tcgctatcgg gtcaaggatt tccatgccgt ggacgtcgag gatcacgttg
    28081 ccattatcgg tcaccacgcc ctgacggtat tccggacgac cgcccagttt caccagctgg
    28141 cgcgccactg cactacgtgc catcgggata acttctactg gcagcgggaa tttacccaga
    28201 atatcaacct gcttggaggc gtcggcaata cagataaatt tttccgcaac cgaagcaatg
    28261 attttttcac gggtcagcgc cgcgccgccg cctttgatca tttgcatgtg gccgttgatt
    28321 tcatctgcgc catcaacgta gatgccaagg ctgtcgactt cgttgagatc aaaaacgtga
    28381 atgccgaggc ttttcagttt ttcagtggaa gcatctgaac tggaaacggc cccttcaatc
    28441 tggcctttca tcgtgccgag cgcgtcaata aagtgtgcgg cggtggaacc tgtacctaca
    28501 ccaacaatgg taccgggctg aacatactga agtgccgccc atcctactgc ttttttcaat
    28561 tcatcctgcg tcatgatcgt ttcgcctgtg gtatgaaatt tcacacgcat tatatacaaa
    28621 aaaagcgatt cagaccccgt tggcaagccg ggtggttaac tcatccataa aatatcgcgc
    28681 aatggcgggc atcccctttc gccccgcaaa taaagcatac aacggtctgg gtatgccgct
    28741 ccacggtgcg aacagaggca ataacgccac gccattcaac ataaccaggg cgcgaagtcg
    28801 ctaagcgaaa tgcaatagcg caaatccctg atcttttaag ctgcgcgttt aaccattgat
    28861 acaaatgtta cccgccgacg gcttcggtat atgcaacctg acacaaaatt gtgtcatagt
    28921 gcaggaaaaa gcatttacca ggagcagaca acagcaatga aacgcccgga ctacagaaca
    28981 ttacaggcac tggatgcggt gatacgtgaa cgaggatttg agcgcgcggc acaaaagctg
    29041 tgcattacac aatcagccgt ctcacagcgc attaagcaac tggaaaatat gttcgggcag
    29101 ccgctgttgg tgcgtaccgt accgccgcgc ccgacggaac aagggcaaaa actgctggca
    29161 ctgctgcgcc aggtggagtt gctggaagaa gagtggctgg gcgatgaaca aaccggttcg
    29221 actccgctgc tgctttcact ggcggtcaac gccgacagtc tggcgacgtg gttgcttccg
    29281 gcactggctc ctgtgttggc tgattcgcct atccgcctca acttgcaggt agaagatgaa
    29341 acccgtactc aggaacgtct gcgccgcggc gaagtggtcg gcgcggtgag tattcaacat
    29401 caggcgctgc cgagttgtct tgtcgataaa ctcggtgcgc tcgactatct gttcgtcagc
    29461 tcaaaaccgt ttgccgaaaa atattttcct aacggcgtaa cgcgttcggc attattgaaa
    29521 gcgccagtgg tcgcgtttga ccatcttgac gatatgcacc aggccttttt gcagcaaaac
    29581 ttcgatcttc ctcctggcag cgtaccctgc catatcgtta attcttcaga agcgttcgta
    29641 caacttgctc gccagggcac cacctgctgt atgatcccgc acctgcaaat cgagaaagag
    29701 ctggccagcg gtgaactgat tgacttaacg cctgggctat ttcaacgacg gatgctctac
    29761 tggcaccgct ttgctcctga aagccgcatg atgcgtaaag tcactgatgc gttactcgat
    29821 tatggtcaca aagtccttcg tcaggattaa tccatcaaat aatgcctgat agcacatatc
    29881 aggcgttgtc ctcacttctt tttgtattcc ttgaatcaca tcaca
//
LOCUS       DAJTYF010000038        28364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTYF010000038 DAJTYF010000000
VERSION     DAJTYF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.038"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..1192
                     /gene="pdeC"
                     /locus_tag="OO590_001833"
     CDS             <1..1192
                     /gene="pdeC"
                     /locus_tag="OO590_001833"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR6637217.1"
                     /translation="YYRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQWGV
                     YDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSYQR
                     LITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQPII
                     DIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGAYL
                     ATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMTPI
                     ILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEHII
                     ELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPAREL
                     TRGQ"
     gene            complement(1195..1518)
                     /gene="soxS"
                     /locus_tag="OO590_001834"
     CDS             complement(1195..1518)
                     /gene="soxS"
                     /locus_tag="OO590_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6637218.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1604..2068
                     /gene="soxR"
                     /locus_tag="OO590_001835"
     CDS             1604..2068
                     /gene="soxR"
                     /locus_tag="OO590_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6637219.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2772..4121
                     /gene="ghxP"
                     /locus_tag="OO590_001836"
                     /gene_synonym="yjcD"
     CDS             2772..4121
                     /gene="ghxP"
                     /locus_tag="OO590_001836"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6637220.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4272..5921
                     /locus_tag="OO590_001837"
     CDS             4272..5921
                     /locus_tag="OO590_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6637221.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6075..7367)
                     /locus_tag="OO590_001838"
     CDS             complement(6075..7367)
                     /locus_tag="OO590_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6637222.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKENDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7545..9194)
                     /gene="actP"
                     /locus_tag="OO590_001839"
     CDS             complement(7545..9194)
                     /gene="actP"
                     /locus_tag="OO590_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6637223.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9191..9505)
                     /locus_tag="OO590_001840"
     CDS             complement(9191..9505)
                     /locus_tag="OO590_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6637224.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9705..11663)
                     /gene="acs"
                     /locus_tag="OO590_001841"
     CDS             complement(9705..11663)
                     /gene="acs"
                     /locus_tag="OO590_001841"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR6637225.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12056..13492
                     /gene="nrfA"
                     /locus_tag="OO590_001842"
     CDS             12056..13492
                     /gene="nrfA"
                     /locus_tag="OO590_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6637226.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDKQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13537..14103
                     /gene="nrfB"
                     /locus_tag="OO590_001843"
     CDS             13537..14103
                     /gene="nrfB"
                     /locus_tag="OO590_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6637227.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14100..14771
                     /gene="nrfC"
                     /locus_tag="OO590_001844"
     CDS             14100..14771
                     /gene="nrfC"
                     /locus_tag="OO590_001844"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6637228.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14768..15724
                     /gene="nrfD"
                     /locus_tag="OO590_001845"
     CDS             14768..15724
                     /gene="nrfD"
                     /locus_tag="OO590_001845"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6637229.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            15744..17462
                     /locus_tag="OO590_001846"
     CDS             15744..17462
                     /locus_tag="OO590_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6637230.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSMLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17455..17838
                     /gene="nrfF"
                     /locus_tag="OO590_001847"
     CDS             17455..17838
                     /gene="nrfF"
                     /locus_tag="OO590_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6637231.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17835..18431
                     /gene="nrfG"
                     /locus_tag="OO590_001848"
     CDS             17835..18431
                     /gene="nrfG"
                     /locus_tag="OO590_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6637232.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18773..20086
                     /gene="gltP"
                     /locus_tag="OO590_001849"
     CDS             18773..20086
                     /gene="gltP"
                     /locus_tag="OO590_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6637233.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20164..20853)
                     /locus_tag="OO590_001850"
     CDS             complement(20164..20853)
                     /locus_tag="OO590_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6637234.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20947..23094)
                     /gene="fdhF"
                     /locus_tag="OO590_001851"
                     /pseudo
     CDS             complement(20947..23094)
                     /gene="fdhF"
                     /locus_tag="OO590_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23292..24758)
                     /gene="mdtP"
                     /locus_tag="OO590_001852"
     CDS             complement(23292..24758)
                     /gene="mdtP"
                     /locus_tag="OO590_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6637235.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24755..26806)
                     /gene="mdtO"
                     /locus_tag="OO590_001853"
                     /pseudo
     CDS             complement(24755..26806)
                     /gene="mdtO"
                     /locus_tag="OO590_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26806..27744)
                     /locus_tag="OO590_001854"
     CDS             complement(26806..27744)
                     /locus_tag="OO590_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6637236.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADIALTKLHLEMTTVRAPFDGRIISLKTSVGQFASAMRPIFTLIDTRHWYVIA
                     NFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSI
                     NWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ"
     gene            complement(27763..28038)
                     /locus_tag="OO590_001855"
     CDS             complement(27763..28038)
                     /locus_tag="OO590_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637237.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(28247..>28364)
                     /locus_tag="OO590_001856"
     CDS             complement(28247..>28364)
                     /locus_tag="OO590_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637238.1"
                     /translation="ADLVKAKRAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6470 a         7177 c         7615 g         7102 t
ORIGIN      
        1 ttattaccgc gatacgcctt ttttctctgg ctataaaatg acctatatgc agcggggaaa
       61 ttatgtggcg gttatcaacc ctctcttctg gagtgaagtg atgtctgatg acccgacatt
      121 gcaatggggt gtgtatgata cggtgacgaa aacctttttc tcgttaagca aagaggcctc
      181 ggcagcaacg ttttctccgc tgattcattt gaaggattta accgtacaaa gaaatggcta
      241 tttatatgcg acaatttatt cgacaaaacg cccaattgca gccattgttg cgacttcata
      301 tcaacgtctt ataacccatt tttataatca tcttattttt gcgttgcccg ccggtatttt
      361 ggggagtctt gttctgctat tactctggct acgtattcga caaaactatt tatctcccaa
      421 acgtaaattg caacgcgccc tcgaaaaaca tcaactttgt ctttattacc agccaataat
      481 cgatatcaaa acagaaaaat gtatcggcgc tgaagcgttg ttacgttggc ctggtgagca
      541 ggggcaaata atgaatccgg cagagtttat tccgctggca gaaaaggagg ggatgataga
      601 acagataact gattatgtta ttgataatgt cttccgcgat ctgggcgcgt acctggcaac
      661 acatgccgat cgctatgttt ctattaacct gtcggcctcc gattttcata cgtcacggtt
      721 gatagcgcga atcaatcaga aaacagagca atacgcggtg cgtccgcagc aaattaaatt
      781 tgaagtgact gagcatgcat ttcttgatgt cgaaaaaatg acgccgatta ttctggcttt
      841 ccgccaggct ggttacgaag tggcaattga tgattttggt attggctact ctaacttgca
      901 taaccttaaa tcattgaatg tcgatatttt gaaaatcgat aaatcgtttg ttgaaacgct
      961 gaccacccac aaaaccagtc atttgattgc ggaacacatc atcgagctgg cgcacagcct
     1021 ggggttaaaa acgatcgctg aaggcgtcga aactgaggag caggttaact ggctgcgcaa
     1081 acgcggcgtg cgctattgcc agggatggtt ttttgcgaag gcgatgccgc cgcaggtgtt
     1141 tatgcaatgg atggagcaat tacccgcgcg ggagttaacg cgcgggcaat aaaactacag
     1201 gcggtggcga taatcgctgg gagtgcgatc aaactgccga cggaaaacgc gggagaaggt
     1261 ctgctgcgag acataaccca ggtccattgc gatatcaaaa atcggacgct cggtggtgcg
     1321 caactcaacg gcggccagta acaggcggcg ttggcgaatg taatcgccaa gcgtctgatg
     1381 cgtcaccgtg cggaacatcc gttgcaagta ccactttgaa tagcctgatt tttttgcgac
     1441 gacatcaatg ttaagcggct ggtcaatatg cacgtcaatc catgcgataa gatcctgaat
     1501 aattttctga tgggacataa atctgcctct tttcagtgtt cagttcgtta attcatctgt
     1561 tggggagtat aattcctcaa gttaacttga ggtaaagcga tttatggaaa agaaattacc
     1621 ccgcattaaa gcgctgctaa cccctggcga agtggcgaaa cgcagcggtg tggcggtatc
     1681 ggcgctgcat ttctatgaaa gcaaagggtt gattactagt atccgtaaca gcggcaatca
     1741 gcggcgatat aaacgtgatg tgttgcgata tgtcgcaatt atcaaaatcg ctcagcgtat
     1801 tggcattccg ctggcgacca ttggtgaagc gtttggcgtg ttgccagaag ggcatacgtt
     1861 aagtgcgaaa gagtggaaac agctttcgtc ccaatggcga gaagagttgg atcggcgcat
     1921 tcatacctta gtggcgctgc gtgacgaact ggacggatgt attggttgtg gctgcctttc
     1981 gcgcagtgat tgcccgttgc gtaacccggg cgaccgctta ggagaagaag gtaccggcgc
     2041 acgcttgctg gaagatgaac aaaactaaag cgccacaagg acaagctgac tcatcgttaa
     2101 ctatgaggcc ggggtgaggg taaatatttg cactggtgct gcctgttccc ctcaccctaa
     2161 ccctctcccc gaaggggcga ggggactgat cgagctcttt tggaattatc cgcaaacaca
     2221 aaactaaagc gccacaaggg cgctttagtt tgttttccgg tctttgtctt tctctctatc
     2281 ccgctggaac acaggagggt ttcccccgac gtcaacacac ctcattcgag cacgtggtgg
     2341 aggttccggt tggtgttgat gctttaattg tatgtcaccg acgtttcttc gccagtgtaa
     2401 aagtatactt tttagccgca atatttttgt catctcagac gattttttat cgcaatccag
     2461 aacggtatac ggctcgataa cgctgcaatc ttgcacatcg acgataacgt ttgcgcatca
     2521 attgcctggt ttttcatcgt caagacaata aaagagaaaa aagcagcaaa cttcggttga
     2581 aaaagccgct atgatcgccg gataatcgtt tgctttttta ccacccgttt tgtatgcgcg
     2641 gagctaaacg tttgcttttt tgcgacgcac caaattgccg caaacctgga gcaggaagat
     2701 aacgtttcgc tggcagggga ttgtccgcca cgcatcttga cgaaaattaa actctcaggg
     2761 gatgttttct tatgtctacg ccatcagcgc gtaccggcgg ttcactcgac gcctggttta
     2821 aaatttcaca acgtggaagc actgtccgtc aggaagtggt tgccgggtta acaacgtttc
     2881 tggcgatggt ctactcggtc atcgtcgttc caggtatgtt gggtaaagca ggcttcccgc
     2941 ctgcggcagt tttcgttgca acctgtctgg ttgccggact cggttctatc gtgatgggtc
     3001 tgtgggctaa tctgccgttg gcgattggtt gcgccatctc cctgacggcg tttaccgcat
     3061 tcagcctggt gctggggcaa catattagcg tacctgtcgc gctgggtgcc gtgttcctga
     3121 tgggggtgct gtttacggta atttctgcca cgggtatccg tagctggatt ttgcgcaact
     3181 tgccgcacgg tgtggcgcac ggcacgggga tcggtatcgg tctgttcctg ctgctcattg
     3241 ccgctaatgg tgtcggtctg gtgattaaaa acccgcttga tggtctgccc gttgcgctgg
     3301 gtgatttcgc gaccttcccg gtgattatgt cactggtagg tctggcggtg atcatcggct
     3361 tggaaaaact gaaagtccct ggtggcattc tgctgaccat tatcggtatc tcaattgtcg
     3421 gtttgatctt cgatcctaac gtccatttct ccggcgtttt cgccatgcct tcattgagcg
     3481 atgaaaacgg caattcactg attggcagcc tggacattat gggcgcgctg aatcctgtag
     3541 tcctgccaag cgttctggcg ctggtgatga cggcagtatt tgatgccacc ggaactatcc
     3601 gcgctgtcgc cggccaggcg aacctgctgg ataaagatgg gcagatcatc gacggcggga
     3661 aagcactgac cactgactcc atgagcagcg ttttctctgg cctggtgggt gcagctccgg
     3721 cagcggtata catcgagtct gcggcgggta cggcggcggg cggtaaaacc gggttgacgg
     3781 ctatcaccgt tggcgtgctg ttcctgctga ttctgttcct ctctccgctt tcttacctcg
     3841 ttccggggta tgcaacggct ccggcgctga tgtacgttgg cctgctgatg ctgagcaacg
     3901 tggcgaaaat cgactttgct gattttgttg atgcgatggc gggtctggtt acggcggtat
     3961 tcatcgtgct gacctgtaac atcgtaacag gcatcatgat cggcttcgcg actctggtga
     4021 ttggtcgtct ggtttccggt gaatggcgca agttgaacat cggtacggtc gttatcgccg
     4081 tggcgctggt gaccttctat gcggggggct gggctatcta atctttcatc ggattctgaa
     4141 aacgggtggc aatggctgcc cgtttttatt ttctccgcac attgtgtgag cttttgcgat
     4201 attctgaaaa aatgagaatt caggcataac gtctttctca gatcataaaa aacatcgcaa
     4261 acagggaacg catggaaatc ttcttcacca tactgataat gaccctcgtg gtctcgctgt
     4321 ccggggtggt cactcgtgtc atgccctttc agatcccgct tccgcttatg caaatcgcca
     4381 tcggtgcgct actggcgtgg ccgacgtttg gtttgcatgt ggagtttgat cctgaactct
     4441 ttttagtctt gtttatcccg ccgttgctgt tcgctgatgg ctggaaaacg ccgacccgtg
     4501 aatttctcga acatggtcga gagattttcg gcctcgcgct ggcgctggtg gtggtcaccg
     4561 tggtcggcat tggcttcctt atttactggg tggtgccggg cattccgctg atccccgcct
     4621 ttgcgctggc ggcggtactt tctccgaccg atgctgtggc gctctccggg attgttggcg
     4681 aagggcgcat cccgaaaaaa atcatgggca ttttgcaggg cgaagcgttg atgaatgacg
     4741 cttccggcct ggtgtcgttg aagtttgccg tggcagtggc gatggggacg atgatcttca
     4801 ctgtcggcgg tgcgacggtc gaatttatga aagtagccat tggcggtatt cttgccggtt
     4861 ttgtggtgag ctggctgtat ggtcgctcgc tgcgattcct cagccgctgg ggcggtgatg
     4921 aacccgcgac gcagatcgtt ctgctgttct tgctgccatt cgcttcttat ctgattgccg
     4981 aacatattgg cgtttcgggc atcctcgctg cggttgccgc cgggatgacc atcacccgct
     5041 ccggtgtgat gcgccgtgcg ccactggcaa tgcgcctgcg cgcaaacagc acctgggcga
     5101 tgctggaatt tgtctttaac ggcatggtgt tcctgctgtt aggtctgcaa ctgccgggta
     5161 ttctggagac gtcgctgatg gcggcagaaa tcgaccctaa cgtcgaaatc tggatgctgt
     5221 ttaccgatat tattctgata tatgcggcgc tgatgctggt ccgtttcggc tggctgtgga
     5281 cgatgaaaaa gttcagcaac cgcttcctga agaagaagcc gatggagttt ggttcgtgga
     5341 ccacacgaga aatcctgatc gcgtctttcg ctggggtgcg tggggcgatc actctggccg
     5401 gtgtgctctc aatcccgctg ctcttgccgg atggtaacgt cttcccggcg cgctatgagc
     5461 tggtgttcct ggcggctggc gtcattctct tctcgctgtt tgtcggcgtg gtgatgttgc
     5521 ctattctgct acaacacatt gaagtcgcgg accattcgca acaattgaaa gaagaacgta
     5581 ttgcgcgagc ggcaacggca gaagtggcga ttgtggcgat ccagaaaatg gaggagcgtc
     5641 tggcggcgga taccgaagag aatatcgata accagttgct tacggaggtc agttctcgcg
     5701 tcattggtaa cctgcgtcgt cgcgccgatg gacgtaacga cgttgaaagt tccgtgcagg
     5761 aagagaacct tgagcgtcgc ttccgtctgg cggcattgcg ttctgaacgt gctgaactgt
     5821 accacctgcg cgccacgcgg gagatcagca acgaaacgct gcaaaaatta ctgcacgatc
     5881 tcgatttgct tgaagcgttg ctaattgagg aaaatcagta agccgaaagc gacgtaacca
     5941 ggcccgaggt aatctgaaaa aggtgcaatc ttttgcacct ttaacaggtt agctcgggcg
     6001 aacattcatg attctaaaac aaataagtta tctctcttgt aaagtcaatt cattggctgt
     6061 tcttcgactt aacctcaatc tgcgtgttgc ataatatctg cagtaatctt catagcgact
     6121 gcattaatat tatcctcagt ttgtgacatg ccagtatgag aggtacaatc ttcttcaaag
     6181 aatttctgta gtgttaccag ggtatcacta tcaatgggga tattttcctc cccacggaga
     6241 atggaaatgc ccattccctg gacgctagcc tcatcgaaat taacccgata gagacgggta
     6301 ttaaacatac aggcttttgt taaaatagaa cagttgaaat ggcagttgct taaattggca
     6361 ttattaaaat taacattagc catattagag ccggtacata taaccatttc gaggaatgta
     6421 tttgtcagcc acgtacaaaa gagtttgcta tcaattaagt ttatattctg gaggattgct
     6481 ttgttcagat tgacgcgaga cagatcagca tgttcaaagt taattaaatc gagtttagag
     6541 aaactaaggt cggctttaaa taaattaaca tatggcgcta ttactttata aagatttacc
     6601 tcagccatgt acgccatata aaaatttgaa tagtcaagga aggtattttt aatattggct
     6661 ctattcattg ctgcattgta gaattgaaca tgcatagcgg agcatgcatt tagagttccg
     6721 tcagccattt ttatctgagc gaaatttgag tcatcgagaa tacaattatc aaaaagtatt
     6781 ttttccatta tggcgtaata aaaattcgtt ttatacatat tacagtcctg gaaaatagcg
     6841 ttttgtagtc tgcaacctgc aaaattcgaa ttggataaat cttcatcttt aatcacgata
     6901 cccttcaggc taaggtgtga aaaatcgtaa ccaccattta ctatctgtgg aatttcgata
     6961 gtattattat ggacggcgac attatttata aaatatacca ccttaatgga tacatcattt
     7021 tctttactaa ggtaaactct tctcgtaatt ctatcgtcac atttattatt tgcatataac
     7081 gctatatcta taaaatattt atgtattttc ctgtccgttt ctttcgtgat ttttcctgat
     7141 ggtaaactat cataaattct tttcattttc tgaatcaagc ctatcacttt ttcatgatca
     7201 tctattttga aaagagagag ggctttattg tcaattaacg tgtttttatg atgtttgctg
     7261 taattatctg attttgtttt ctttgtatgt gatgaacttg tgatagagtg gttatcgtta
     7321 atctggcaaa gagggaaaaa catattattt aaaccattat agcgcataaa atatccttat
     7381 aattaacaag aaagaaaagg catattctct gcattatcat tttctacgac tgtcaaaaat
     7441 cgctcatttt ttaatgagtt tatttgttta atattatggg aaaaggtgat gcatgttgga
     7501 gaggaagagt attccccggt caggcgaccg gggaagggga gagattaatg tgcgcggcct
     7561 tgctcaacgc caaagccggt ctgggagcgg ataaactgcg cgcggaacag ttcacgctcg
     7621 cgcgcccctt ccgctgagtt atcggttgcc gagaagaacc agatgccgag gaatgccacg
     7681 ctgatagaga acagcgccgg gtattcatac gggaagatgg ctttttcgtg accgaggatc
     7741 tgtacccaaa tagtcgggcc gaggatcatc agcaccactg cggtaatcag ccccagccag
     7801 ccgcccagca tcgcgccacg ggtagtcagt ttcgaccagt acatggaaag cagaatgatc
     7861 gggaagttac agctcgccgc gatggcaaac gcaaggccta ccataaaggc gatgttctga
     7921 ttctcaaaca gcacgccgag gataatcgca atcacgccga ggatcagtac ggtgattttt
     7981 gatacccgca gctcttcacg ttcggtcgcg ccttttttga agacgttagc gtacaagtca
     8041 tgcgaaaccg ccgatgcgcc cgccagcgtc agacccgcaa ccaccgcgag gatagtggcg
     8101 aaagcaaccg ctgaaataaa accgaggaat aggttgccgc ccactgcatt cgccaggtga
     8161 acggccgcca tgttgttacc accaatcaga tggcccgccg cgtctttata ttccggattc
     8221 gcaccaacaa gcatgatcgc gccgaagccg ataataaagg tcagaatata gaagtagccc
     8281 ataaatccgg tggcgtagaa cacgctctta cgtgcttcgc gggcatcgct gactgtaaag
     8341 aagcgcatca gaatgtgcgg caagcccgcc gtaccaaaca tcagtcccag ccccagagag
     8401 agcgcggaga tcggatcttt caccagtccg cccggtttca tgatgtcgac acctttcggg
     8461 tgtaccgcca tcgcttcgct gaacagattg ttgaagctaa agccgacgtg tttcatcacc
     8521 ataaaggcca taaagctggc accgaacagc aacagcacag ctttgataat ttgtacccag
     8581 gtggtcgcca gcatgccgcc gaacaggacg tacatcatca tcagcacgcc gaccagcacc
     8641 accgcaatgt gatagttaag gccaaacagc agctcgatca gtttacctgc gcctaccatc
     8701 tgagcgataa ggtaaagcgc caccaccacc agagaaccac aggccgaaag aatacgaatc
     8761 ggcccttgtt tcagacgata agaggccaca tcggcagagg tgtagcgtcc caggttacgc
     8821 agacgttcgg taatcagaaa cagaatgatt ggccagccca ccaggaagcc caacgagtag
     8881 atcagcccgt catagccgga ggtaaacacc agcgcggaga tccccaagaa tgaggcggcg
     8941 gacatatagt ccccggcaat cgccagcccg ttctggaagc cagtgatatt gccgcctgcg
     9001 gtgtagtagt cgctacgaga acgtacgcgt tttgatgccc agtaggtaat gccgagcgta
     9061 aacacgacga aaatcaggaa cataataatc gcctgccagt tcgttggctg gcgctctacg
     9121 gccccgctaa tagcatccgc ggcgttagct gcgaaaggga gtgtggcggc aagcgccgtc
     9181 agaactcttt tcatgatgct tgtacctcat gcaggacttc attattaaga cggtcgaatt
     9241 cgccgttcgc ccgccagatg tagataccgg tgagaacaaa ggagatcaca atcactccaa
     9301 caccaattgg aataccgcgt gtgacgctgg tgttcggatt cagcggcgtg cccagccagc
     9361 cgggcgcgaa ggcgatcagt aaaataaagc cgatataaac tgccagcata ataatcgaca
     9421 ggatggtggc aaaccgttgc cgtttttcga ctaactccct gaaatgcgca ttgtcttcta
     9481 tccgctgata aatcgtacca ttcatcacag attctccaga ggtaatgtag ggattgtttt
     9541 aattcccgct cccttatggg agaaggttaa cgctcgggta acccttgccg aatgtaggcc
     9601 ggataaggcg tttacgccgc atccggcaat caatgcctga tgcgacgctg tcgcgtctta
     9661 tcaggcctac aaaccgttac cgattcgcat ccggcaattg tgggttacga tggcatcgcg
     9721 atagcctgct tctcttcaag cagcttctcg accacgccag gatcggcaag cgtcgaggta
     9781 tcgcccaggt tgctggtatc gcccgccgca attttgcgca gaatacggcg cataattttg
     9841 ccggagcggg ttttaggcag ggagtcggtc cagtgcagca cgtctggcgt cgccagcggg
     9901 ccaatctctt tacgcaccca gttgcggact tctgcgtaca gttctggtga cggttcctcc
     9961 ccgtgattaa gcgtgacata ggcgtagatc gcctgacctt taatattgtg cggaatacct
    10021 actacggcgg cttcggcaat cttcggatgc gccaccagcg ccgactctat ctctgccgtc
    10081 cccagacggt gaccggagac gttcagcacg tcgtccacac gcccggtgat ccagtaatag
    10141 ccatcttcat cgcgacgcgc gccgtcgccg ctgaaataca tatttttgaa ggtggaaaaa
    10201 taggtctgct caaaacgttc gtgatcgcca aacagcgtac gcgcctgacc aggccaggag
    10261 tcggtgatca ccaggctacc ttcggtagcc ccttccagcg ggttaccttc gttatcgacc
    10321 agcgccggtt gcacgccgaa gaacggacgt gttgccgaac cggctttcag ctcggtagcg
    10381 ccaggcagcg gggtgatcat gaaaccgccg gtttcggtct gccaccaggt atcgaccacc
    10441 ggacatttct cgttgccgat ttttttccag taccactccc acgcttccgg gttaattggc
    10501 tcgcccacgg aaccgagaat gcgtagcgac gaacggtcgg tgccttcaat cgctttatcg
    10561 ccttccgcca tcagcgcgcg gatcgccgtg ggtgcggtat agagaatatt gacctgatgc
    10621 ttgtccacca cctgcgccat acgggcaggc gtcggccagt tcggtacgcc ttcaaacatc
    10681 agcgtggtcg cgccgcaggc cagcgggccg tacagcaaat aactgtgtcc ggtaacccag
    10741 cccacatcgg cggtgcacca gtagatatca cccggatgat aatcaaagac atatttaaag
    10801 gtcagcgccg cgtacaccag ataaccgccg gtagtgtgca gtacgccttt tggttttccg
    10861 gtagaaccgg aggtatagag aataaacagc ggatcttcgg cgttcatctc ttccgcctgg
    10921 tgctgatcgc tggcttgctc aaccaggtcg tgccaccaca ggtcgcgccc ttcctgccag
    10981 tcgatgtttc cgccagtacg cttcagtacc accacatgct ctacgctggt gacgttcggg
    11041 ttttttagtg cgtcatcaac gtttttcttc agcggaatac tacgcccggc gcgcacgcct
    11101 tcgtcggaag tgatcaccag tcgtgagttg gaatcaataa tgcgcccggc aacggctttc
    11161 ggcgagaagc cgccgaaaat caccgaatgc accgcgccaa tgcgggcgca ggccagcatc
    11221 gcaaccgcgg cttccggcac catcggcata taaatcgcca ccacatcacc ttttttaatg
    11281 cccagctcga gcagggtatt ggcgaagcgg cagacgtcgc ggtgcagctc tttatagctg
    11341 atatgtttgc tctggctggc gtcgtcgcct tcccagatga tggcggtgcg atcgccgttt
    11401 tcttgcagat ggcggtcaag gcagtttgcc gccagattca gcgtgccgtc ctcgtaccat
    11461 ttaatggaca cattaccggg ggcaaaggag gtatttttca ccttctggta cggtttgatc
    11521 cagtcgagaa tttttccctg ttcgccccag aaggtatcag gtacgttaat agattgttga
    11581 tacatcgcct cgtactgctg agggtttatc aggcaacggt ctgcgatgtt ggcaggaatg
    11641 gtgtgtttgt gaatttggct catgcttttg ttctccttgt aggatgttaa taatatgtgg
    11701 cataagcgtt aaatgtaggg gtattggcag ttttgtttag tatttgggcg acagatcacg
    11761 caaaagtaga atggtgcaaa taaacggcag ggtaattttt gaaggtcacc aacaaaagtt
    11821 gattaattct ttgaggaaca tgcagttatg catgctgttg aaaaagagga agatactaac
    11881 taactctaaa gtggtatttt acatgcactt acaattgatt aaagacaaca ttcacagtgt
    11941 ggttatttgt tacacatagg ggtgagcaat gtcatgacag tgtaggtgcg gttactgtcg
    12001 tgaaaaacaa taaaaaccgc cattgcaaca atggcgcaat tcggatgaag cccctatgac
    12061 aaggataaaa ataaacgcac gccgtatctt cagcttattg attccttttt tctttttcac
    12121 ttctgttcac gctgaacaaa cggcagctcc cgcaaaacct gtaactgtgg aagcgaagaa
    12181 tgaaaccttt gccccgcagc atcacgatca atatctctcc tggaaagcca cctcggaaca
    12241 gtcagagcgt gttgacgccc tggaggaaga tccacggctg gtgatcctgt gggcggggta
    12301 tcccttctcg cgcgattaca acaagccgcg tggacatgcc tttgctgtga ccgatgtgcg
    12361 tgaaaccctg cgtaccggtg cgccgaaaaa cgctgaagat ggtccgctac cgatggcgtg
    12421 ctggagttgt aaaagcccgg atgtggcgcg tctgatccag aaagacggcg aagatggcta
    12481 cttccacggt aaatgggcgc gcggcggccc ggaaatcgtc aacaacttag gttgtgccga
    12541 ctgccataac accgcctcac cagagttcgc caaaggcaaa ccggagttaa ccctttcccg
    12601 tccgtatgcg gctcgcgcga tggaagccat tggtaaacct tttgagaaag ccggacgttt
    12661 cgacaagcaa tcgatggttt gcggtcagtg ccatgtggag tattacttcg acggcaaaaa
    12721 caaagcggtt aaattcccgt gggatgacgg catgaaagtc gaaaatatgg agcagtatta
    12781 cgacaaaatt gccttctctg actggactaa ctccctgtcg aaaacgccaa tgctgaaagc
    12841 gcagcacccg gaatatgaaa cctggacagc gggcattcac ggtaaaaaca acgtgacctg
    12901 tatcgactgc catatgccaa aagtgcagaa cgccgaaggc aaactctaca ccgaccataa
    12961 aattggtaat ccgtttgata acttcgccca gacttgtgcg aactgccata cccaggataa
    13021 agctgccttg caaaaagtgg tcgcggaacg taagcaatcg attaacgacc tgaaaatcaa
    13081 ggttgaagat caactggttc acgctcactt cgaagcgaaa gcggcgctgg atgcaggcgc
    13141 gacggaagcc gaaatgaagc cgattcagga cgatatccgt catgcccagt ggcgttggga
    13201 tctggcgatc gcttcccacg gtattcatat gcacgcaccg gaagaaggtc tacggatgct
    13261 cggtacggcg atggataaag cggcggatgc gcgcaccaaa ctggcgcgcc tgctggcgac
    13321 caaaggcatc acccatgaaa tccagatccc ggatatctcg accaaagaga aagcccagca
    13381 ggccattggc ctgaacatgg aacaaatcaa ggccgagaag caggacttca tcaaaacggt
    13441 gatcccgcag tgggaagagc aggcacgtaa aaacggtctg ttaagccaat aaccccgttc
    13501 cgcctcgcaa ggggcggaaa acacaatgga gtgaatatga gcgtattacg ttcgttatta
    13561 actgctgggg tgctggcgtc gggcctgttg tggagcctga acggaattac cgctacccct
    13621 gcggtgcagg catctggtga tcgctacgaa gttacccagc agcgtaaccc ggatgccgcc
    13681 tgtctggact gtcataaacc agataccgaa ggtatgcatg gcaaacatgc ttccgttatc
    13741 aacccgaata acaaattgcc ggtcacctgc accaactgtc acggccagcc atcaccgcaa
    13801 caccgcgaag gggtgaaaga tgtgatgcgc tttaacgagc cgatgtacaa ggtcggagag
    13861 cagaacagcg tctgtatgtc ctgtcatctg ccggaacagt tgcaaaaagc gttctggccg
    13921 cacgatgtcc acgtaaccaa agtggcgtgc gccagctgcc attccctgca tccacagcaa
    13981 gatacgatgc agacgttaag tgacaaagga cggattaaga tttgcgtcga ttgccacagc
    14041 gatcagcgca ccaatccgaa ctttaacccg gcgtctgttc cgttgcttaa ggagcagcca
    14101 tgacctggtc tcgtcgccag tttctcaccg gcgtcggcgt gctggcagcc gtcagcggca
    14161 ccgcagggcg cgtggtggcg aaaacgttga atatcaatgg ggtgcgttac ggcatggtgc
    14221 atgacgagtc gttatgcatc ggctgtaccg cctgtatgga tgcttgtcgg gaagtgaaca
    14281 aggtgccgga aggcgtctcg cgcctgacga ttattcgtag cgagccgcag ggcgaatttc
    14341 ctgatgtgaa atatcgcttc ttccgtaagt cttgccagca ctgcgagcat gcgccgtgcg
    14401 ttgacgtctg cccgaccggt gcgtcttttc gtgatgccgc tagcgggatt gtcgatgtta
    14461 atcctgatct ctgcgtcggt tgtcagtact gcatcgccgc ctgtccgtac cgcgtgcgct
    14521 ttatccatcc ggtcacgaag acggcggaca aatgcgattt ctgtcgtaag accaatttgc
    14581 aggccggtaa gcttcccgcg tgcgttgaag cttgcccgac gaaggcgctg acgtttggca
    14641 atctggacga tcctaacagt gagatttcgc aactgctgcg ccagaagcct acttaccgct
    14701 acaagctggc gctgggaacc aaaccgaagc tgtaccgcgt accgtttaaa tacggggagg
    14761 tgagccaatg acgcagactt ccgcatttca ttttgaatcg ctggtgtggg actggccgat
    14821 tgccatctac ctgtttttga ttggtatttc tgccggtctg gtgacgctgg ccgtgctgtt
    14881 acgtcgcttc tacccgcagg cgggcggtgc agacagtacg ttgctgcgca ccacgctgat
    14941 tgtcgggccg ggcgcggtga tcctcggtct gttgatcctc gtcttccacc tgacaagacc
    15001 gtggaccttc tggaagctga tgttccacta cagttttacc tcggtgatgt cgatgggggt
    15061 gatgctgttt cagctctaca tggtggtgct ggtgctgtgg ctggcgaaaa tctttgaata
    15121 tgatttgctt gccctgcaac aacgctggtt gccgaagctg gggatcgtgc aaaaggttct
    15181 gagccttctg acgcccgttc atcgcggact ggaaacgctg atgctggtgt tggcggtgct
    15241 gctgggggct tataccggct ttctgctgtc ggcgctgaaa tcgtatccgt tcctcaataa
    15301 cccgagcctg ccggtgctgc tcctcttctc cggcatctcg tccggtgcgg cggtggcgct
    15361 gatcgccatg gcgatacgcc aacgcagtaa cccgcattcc acggaagcgc actttgttca
    15421 ccggatggaa atccccgtgg tatggggtga aatcttcctg ctggtggcgt tttttgtcgg
    15481 tctggcgctg ggcgatgacg gtaaagttcg tgcgctggtg gcggcattag gtggcggttt
    15541 ctggacgtgg tggttctggc ttggtgtcgc cgggctgggg ctgattgtgc caatgttgct
    15601 caaaccgtgg gtcaatcgca gttccggcat tcctgccgtg ctggcggcgt gtggggccag
    15661 tctggtcggc gtgttgatgc tgcgcttttt cattctctac gccggacagt tgacggtggc
    15721 gtaagccaga aaagaggtgg tttctggacg tattccttcc tgaagtcggt tttctggcgt
    15781 tgttgttaag tctcggggtc aacgtgttga ccccgttgac ggcctttgcg ggagtgcggt
    15841 tgcgctggcc tgccatgatg cgactcactt gcatcggcat tctggcgcag ttcgcgctcc
    15901 tgctgctcgc ctttggcgta ctgacgtatt gttttctcat cagcgatttc tcggtcattt
    15961 atgtcgccca acatagctac agcatgctgt cgtgggaact caaactggcg gcggtgtggg
    16021 gcggtcatga aggttcgctg ctgctttggg tgctgctgct ttccgcctgg agcgcgctgt
    16081 ttgcctggca ttatcggcag caaagcgatc cgctatttcc gctgacgcta gccgttttat
    16141 ctctcatgct cgccgcactg ctactgtttg tggtgctgtg gtccgatccc ttcgtgcgga
    16201 tatttccacc agcaatcgaa ggccgcgatc tcaatccgat gctgcaacat cccggtctta
    16261 tctttcatcc accgctgctt taccttggct atggcggttt gatggtagcg gcgagcgtgg
    16321 cgctggcgag tctactgcgc ggcgagtttg atgcggccag cgcccgaatt tgctggcgct
    16381 gggcgttacc tggctggagt gcattaacgg cggggatcat cctcggttcc tggtgggcct
    16441 actgcgaact cggctggggc ggctggtggt tctgggatcc ggtggaaaac gcctctttat
    16501 taccctggct ttctgccact gcgctgctgc acagtttgtc cctgacacgc cagcggggga
    16561 ttttccgcca ctggtcgctg ttgctggcga tagttactct gatgctgtcg ctgctgggca
    16621 ccttaattgt ccgttctggc attctggttt cggttcatgc gttcgcactg gataacgtcc
    16681 gcgccgtgcc gttgttcagc ctgtttgcac tgattagcct tgcgtctttg gctctgtatg
    16741 gctggcgagc gcgggacggt ggcccggcgg tgcgtttttc ggggttatcg cgggaaatgt
    16801 taatcctcgc tacgctgttg ctgttttgcg cagtgctact gatcgtgctg gtgggaacgc
    16861 tttatccgat gatttacggc ctgctgggct ggggacgcct ctccgttggc gcgccgtatt
    16921 ttaaccgcgc gacgttaccg tttggtctgt tgatgctggt ggtgattgtg ctggcgacgt
    16981 ttgtctctgg caaacgcgtg cagcttccgg cgctggtagc tcatgctggc gtgctgttat
    17041 ttgccgcggg gatcgtggtt tccagtgtca gtcgtcagga gatcagcctg aatttacagc
    17101 cgggtcagca ggtgacgctg gcaggataca ccttccgttt tgagcgactc gatctgcaag
    17161 ccagaggcaa ttacaccagc gaaaaagcga tagtggcgct gtttgaccat cagcaacgca
    17221 ttggtgaatt aacgccggag cggcgttttt atgaagcacg ccgtcagcaa atgatggaac
    17281 cgtcaattcg ctggaacggc atccatgact ggtatgcggt catgggtgaa aaaaccagag
    17341 cggatcgtta cgcttttcgt ttgtatgtac aaagcggtgt gcgctggatc tgggggggag
    17401 gattgttgat gattgcgggc gcattgttaa gcggatggcg ggggaagaag cgcgatgaat
    17461 aaagggcttc tcacgctact gctgttattt acctgttttg cgcgcgctca ggttgtagac
    17521 acctggcaat tcgccaatcc gcaacaacag caacaggcgt taaatattgc cagccagtta
    17581 cgttgtccgc agtgccagaa tcaaaactta ctcgaatcca atgcgccggt ggcggtcagt
    17641 atgcgccatc aggtttatag catggtgtcg gaggggaaga acgaagtcga aatcattggc
    17701 tggatgaccg aacgctacgg agattttgtt cgctataacc caccgttaac gggtcagacg
    17761 ctggtgttgt gggcgctgcc agtggtgttg ttattgctga tggcactgat cctctggcga
    17821 gtgagggcta agcgatgaaa cagcctcaaa taccggtgaa aatgctgaca acactcacga
    17881 tattgatggt attcctctgt gtcggcagtt atctgttaag cccaaaatgg caggcggtac
    17941 gtgcggagta tcagcgtcag cgcgatccgc tacatcagtt tgccagccag caaaccccgg
    18001 aagcgcagct tcaggcattg caggataaaa tccgcgctaa tccacaaaac agcgaacaat
    18061 gggcgttact gggcgagtac tatctgtggc aaaacgatta cagcaattcg ctgctggcgt
    18121 atcgtcaggc gttgcaacta cgtggtgaga acgctgaact gtatgcggcg ctggcgacgg
    18181 tgctttatta ccaggccagc cagcatatga ccgcccagac tcgcgcaatg atcgacaaag
    18241 ccctcgcgct ggactctaac gaaatcaccg ctctgatgct gctggcttcc gatgcgttta
    18301 tgcaggcgaa ttacgcgcaa gccatcgaac tatggcaaaa agtgatggat ctcaactcac
    18361 cccggatcaa ccggacacag ctggtcgagt cgattaatat ggcaaaactg ttgcagcgga
    18421 gatcggatta atcatctgtt gatgaagatt atgtcattga gctgcataaa aaataatcga
    18481 atgaacatat gccaaaaata atcaccaatc agtattattg cagattaaac aaataaaaat
    18541 ctttccataa caaatggtta ttcattaatc ctgccataaa ctcgcgttat tatgcattaa
    18601 tgcagcgaaa agctctgttg ttaaagggtt gcgcaacata ccgcgcaaat gatactgatc
    18661 ataagcgtta aaaaaatcta caaaccaacg caacacaatt catgccctgg cagtatgtca
    18721 cgttctcgcg tttctgaacg gggaacggcg ctccattgag gaagttattc tgatgaaaaa
    18781 tataaaattc agcctggcct ggcagattct gtttgctatg gtgctgggca ttctcctggg
    18841 aagctacctg cactaccata gcgacagccg cgactggctg gtcgtcaatt tgctctctcc
    18901 ggcgggtgat atcttcatcc atctgatcaa aatgattgtt gtgccgattg tgatctccac
    18961 gctggtggtg ggtatcgcgg gtgttggtga tgccaaacag ctcgggcgta ttggcgcgaa
    19021 aaccattatt tacttcgagg tgatcaccac cgtcgccatc attttgggga tcactctggc
    19081 gaacgtcttc cagcccggtg ccggggtgga tatgtcgcag ctggcgaccg tcgatatctc
    19141 gaaatatcag agcactacgg aagcggtaca aagcagttcc cacggcatta tgggcacgat
    19201 tttgtcgctg gtgccgacga acattgtggc gtcgatggcg aaaggcgaaa tgctgccgat
    19261 catctttttc tcggtgctgt ttggtctggg gctttcttcc ctgcccgcga cgcatcgtga
    19321 accgctggtg accgtgttcc gctccatctc tgaaaccatg tttaaagtga ctcacatggt
    19381 gatgcgttat gcaccggtgg gtgtgtttgc gctgattgcg gtgacggtgg ctaactttgg
    19441 tttctcgtct ctgtggccac tggcgaaact ggtgctgctg gtgcatttcg ccattctgtt
    19501 cttcgcgctg gtagtgctgg gaattgtggc acgtctgtgc gggttaagcg tctggagcct
    19561 gattcgtatt ctgaaagatg agctgattct ggcgtactcc actgccagct ctgaaagcgt
    19621 actgccgcga attattgaga agatggaagc ctacggagca ccggcgtcga tcaccagctt
    19681 cgtggtgccg accggttact cttttaacct tgatggttcg acgctgtatc aaagtattgc
    19741 cgctattttc atcgctcagc tgtatggcat tgacctgtcc atctggcagg aaatcattct
    19801 ggtgctgacg ctgatggtga cctcgaaagg gattgctggc gtggctggcg tgtcgtttgt
    19861 ggtgttgctg gcaacgctgg gtagcgtagg tattccgctg gaaggtctgg cgtttattgc
    19921 tggtgttgac cgtatcctcg acatggcgcg tactgcgctg aacgtggtgg gtaatgcgct
    19981 ggcggtgctg gtgattgcca agtgggaaca caaatttgac cgcaaaaagg cgctggctta
    20041 tgagcgtgaa gtgctgggca aatttgataa aactgcggat caataattga agattgccgg
    20101 ggatatcccc cggcaatgtg tggatgcctg atgcgacgct tgccgcgtct tatcaggcct
    20161 accttaaccg ttggttaatt tttcaaactc ttcacacccg gtatcaaacc cttccataca
    20221 gctcaggttc aaccagtgca gcgccttttg tttgttcttc tcgataaaac cttcctcacc
    20281 gtttaagaac atcattcccg cccagtactc ggaataaccg gttcgggaaa ttgcagagct
    20341 gcgtttgaaa taccaggttg ccttgtcatc gtcggcctta atgcccacgc cgttggcgta
    20401 aatcaaacca agcagcattt gggcatcgac ggcggagtca ttctccagat cttccgaggc
    20461 gttttctaac agcgaaatcg cctttggata atccggttta cccgcttgag tatttaccag
    20521 aatatgcgcc agcgttactt caccttcttt actgcccgct tgcgccgctt tctctgcaag
    20581 aacttttgct tgtggatagt ccagactgac cggattggtg attttgatct gcgccaacag
    20641 cgcgcaggca tcagcatcac cgctgtcggc ggctttctgt gcccaatatt cggctttgct
    20701 caaatcgccg gagctaaacc agctgtcggc aagaaaatat tgtgcgcgtt ggtccccggc
    20761 ctctgctgcc tttaaatact ggctgccagg ctcggagtcg ttcgcgtggg caaagaatgt
    20821 caaaaacaac atcaatgcga taattttttt cattttgata atacgattgg gtagggagta
    20881 accagtataa agagttcgaa tggataaaaa aacagcctcc gaaaggaggc tgtagaaagg
    20941 actgtattac gccagtgccg cttcgcgcag gcgagttttc aacttgttgt actcgtcaat
    21001 caggtactgc tcggcggcgc gctgatcgga gatcggctcg acgcgcacgg cgcagtattt
    21061 gtattccggc gttttcgtaa tcgggcttaa gttttcggta accagctcgt tacaggcacc
    21121 aatccaccac tggtaggtca tgtaaatcgc ccctttgttc ggacgatcgc tgacctgcgc
    21181 acgggtgata attttgcctt tacgcgagtg cactcaaacc aatgcctcat cttcaatacc
    21241 cagacgtttg gcgtcttcgg tattgatttg tgcgtagcca ggttcatcag ccagcgccgc
    21301 cagtgccgca cagttaccgg tcatcgaacg gcaagagtag tgaccaactt cacgcaccgt
    21361 tgacagtacc atcgggtact cgtcggtgag tttgtcgatt ggcgctaccc agtcgcaggt
    21421 gaagaactgc gccagaccgt tcggggtatc aaacttctct ttaaacagat aagaagtccc
    21481 ctgatcggca tctgaagtat cgcggcaagg ccactgaatg aagcccagtt cgcccatttt
    21541 ctcgtaagtc gcaccgtaga aatccgggca cagatgacgc aactcatccc agatctcctg
    21601 ggtgttgttg tagtgcatcg gataacccat acgggtggcg atttcactga tgatttgcca
    21661 gtccgttttc agatcccatt tcggttcaac tgctttgaag aaacgctgga agccacggtc
    21721 agccgcagta aacacgcctt catgctcgcc ccacgacgtt gacggcaaaa tgacatccgc
    21781 cgccgacgcg gttttggtca taaagatgtc ctgaacgatg accagttcca gatcttcaaa
    21841 ggctttacgt actgccgaca gctccgcatc agtttgcagc ggatcttcgc ccataatgta
    21901 cgcggcacgc acttcgccat gcgctgcgcg gtgcggcagc tcgctgatgc gatagccggt
    21961 atgagcaggc aggctttcca cgccccaggc tttggcgaat ttctcgcggt tagccggatc
    22021 tttcacgtac tggtatcccg gatacgtatc cggcagcgcg cccatatcgc aggcaccctg
    22081 cacgttgttc tgaccacgaa ccgggtttac acccgcatgc ggcttaccga ggttaccggt
    22141 cagcatcgcg aggctggtca gagaacgcac ggtttccacg ccctggtaga actgggttac
    22201 acccatgccc cacaggatgg cggcgctttt cgcctgggca tacatccgtg ccgcctgacg
    22261 aatctcactg gcgctgacac cggtgatatc ttcaaccgac tccggcgtgt agccttcaac
    22321 gattttacga tactcttcaa agccttctgt acgggaagcg acgaacgctt tgtcgtacag
    22381 attttcttca ataatgacat ggcccatcgc attcaacagc gcgatgttcg agccgttttt
    22441 cagtgcaatg tgcatgtcag caatgcgcgc ggtttcaatt ttgcgcggat cgcagacgat
    22501 aattttcgcc ccgttacgtt tagcgttaat tacgtgattc gccacgattg ggtgggaatc
    22561 cgccgggttg tacccgaaaa cgaacactaa atcggtatta tcaatttcgt taatagcatt
    22621 gctcattgcg ccattaccga ccgattggtg cagacctgca accgatgggc cgtgtcagac
    22681 acgagcgcag cagtcaacgt tattggtacc aataacggcg cgcgcaaatt tttgcattac
    22741 atagttggtt tcgttacccg taccacgcga ggagccggtc gtctggatgg catccggacc
    22801 gtacttctct ttgatggcgc tcaggcgctc ggcaacgtaa ttcagtgcct catctcagga
    22861 aacaggttcg agtttgccgc cacgctggcg acggatcatg ggggttttca ggcgcggggt
    22921 caggatctgg gtatcgttaa tgaaatccca gccataataa cccttcagac acagggtacc
    22981 ctggttggtt ttcccctgcg ctgcctccgc ccggacgatt ttgccgttat cgacgaccag
    23041 gttgattttg caacctgatg cgcaataggg gcaaaccgtg acgacttttt tcatcggtct
    23101 cgctccagtt aatcaaatca cgcatacgcg ctctcgacta cagtatgcat cttttatgcc
    23161 acattttatt tggggtcatt ccctgatatt acgggcacta tttattcaaa actctgacga
    23221 aaaacaggct gtcgtcagtt ttgacgtgac gaaacgaaat accgcgtgac agccatcacg
    23281 cggcagacat tttatttttt ctcgacgacg ggagccgcct gatacccgcc gcccagcgat
    23341 ttcatcaact gaatgctttg gatcacccgg cggctgtcca gcatcagtaa tgacatctct
    23401 tcggcaagta ctggcaaccg ggcttcagtg gcctgtaagc ggctggttaa gccgcgctga
    23461 taggcggcct cggcagcgcg ctgggtgaag cgcgttgctt ccacgtgttc agcctgcatt
    23521 tctcgctcgt cgttgagcgt ttgcagacgc gtgccgttga cggcaacgtc acgcaccgcg
    23581 ttcagtactg actggttgta acgttcaatc atcatgttgc tggcggcgcg cgtgccttcg
    23641 agattggcat tcaaccgtcc accgtcaaac agcggcaatt tcagacccgg gatgaagttg
    23701 aactggcgac tggttttttt gaataaggta tctaggtgga tggcatcaag gccgaaaaac
    23761 gctttgatat caaagctcgg atagaacaac gcccgcgcgg aatccacctg atctaatgac
    23821 gcctgaacat accagcgcat ggcttgcaga tccgggcgtc tggcgagcaa ctcataagag
    23881 agtgtcgccg gaatgccggt ctggactcgt ggtaatgcca caggtttgat ctccggcata
    23941 tcgctggctc ccgcgccaat caatgcgcgc agagattctc tcgtttcggt gatttgccct
    24001 ttgacggcag caatttgttt atcgaccgcc agaatctgtg cccgcgcgcc gtggaaaggc
    24061 acttgtgctt ccagaccgtg cgccacttta ctttggtgcg ctttcaccgc gtaatcaatc
    24121 acatcgcgag tttgttctaa cagatcgagc atctgatagc tggcctgcat actgtaataa
    24181 agctgcgcta cgcccgtggt cagcgatagc tctactgctg cggtttctgc cagcgcggca
    24241 ttatgcgcgc caatggcggc ggcaaccgct gagcgatgca caccccacaa atcaagatcc
    24301 agtccggcaa acaaacctac tgtggcttcc gtatagtacg gcccgtccat ccccagtgcg
    24361 ggcgcatcca tcgcataagg gcttaaaaag ccgttcgccg agacacgttg gcggttaagc
    24421 atccctaacg ctgccacctg caactgtgaa ccggcatcta acaaatcggc ctgcgattgc
    24481 gctttttctt cccgcagttt cgcttcggcg agggtgtgtg aaccacttag cgtccgttgg
    24541 atcagggcat ccaactgcgg gtcattgagt tgtttccacc actgcgcctg cggccagccg
    24601 gagctggcaa gatgaatatc atcggccagt ttgatttgtt ccggtttgag ctgttgatga
    24661 ggcgcagaat ccttacgtac cagggcacag ccggaaatca gcgtcgtgct gccgagaatg
    24721 ctgcacaaca gcagacgtga aagttgacga ttgatcattg cgtggctcct tgtgcctgtt
    24781 ccgtggcggg cgttaatgcc ggggctgtcc agtctggcaa gcgggcgaaa agctggcaga
    24841 cgtgttgctc ctgttttaat aaggtgggca ggatggcctg cgaggcgggt gtctcttcaa
    24901 gcgttatttg aggagaacgg ctgagtgcgg ttgccagacc ggcagcgtat ttctccaggg
    24961 cgtcggcaaa ctgacctgct ctgtccggct gtagtgcgtt atccagcgcc tgcgccgaat
    25021 tccaggtggc atcccaggcg tgaaggatat cgcggccctg acgaataacc gtttgcgaac
    25081 gttcaatcag taaggcgcgt tcttcgctgt ccagttgacg ctccagcgcc acgcgttggc
    25141 acatctcttc acaggcatta aacgccgcat gcagaccgat acgaatttgc agataagtgc
    25201 gcagagccgt gacttcctgc tggcgtggga tccgcattac tttacttaac atacccagcg
    25261 cgccagccag tttttgcggc agcgtgcgcg cttcactttc aggccagaca aaggtgtaaa
    25321 tcatcgcgga caccacggta ccaatgatga tacccagggc gcgatcgcga atttccacca
    25381 ggtcgtacac tgggccaaaa acgttttcga gcgtggcgag cgcgaaggtg accaccatct
    25441 gtgtgccgat ataagaagag cgttcagagc tggtggcaat ctatgcgccc aacaggaaaa
    25501 tcggtgccag cacaaacagc aattcgacaa tattgtccag ccagggcatg accagtagcg
    25561 tgaataacag cgccagaatc gcgccgcaaa aggccccgcc aaaacgcagc accatcttct
    25621 ggtacgacga accgacattt ggattagcga cgatcacgca ggtcagcata caggtgtgaa
    25681 tgccttccca gtccacgccg ctgtaaaagg tgtagcagat caaacaggcg agcaatgttt
    25741 ttaccgcgta gcgtatatag tctggattgg taaaagcatc ggcgaccatt gatggcggtt
    25801 tggcggcggg cgttggcggc gtattcgggt tcatctggcc cagttgtaac aacgtctggc
    25861 agatattctc caggttacat tcccgtgccg ccaccgcttc actttcactg atccgccagt
    25921 cgctttgcca gcactgacct tcagcaacgg catgctgcag cttgttgatt tctgaggcta
    25981 atttttgccg aaattcaata attgcctgag aatcagcaaa agaggtggga tcgtagcgat
    26041 tcagcgtcga gtaaatgtag gttaccgttg ccacgcagct ttgccaccat gcgctttgag
    26101 ttcgccagtt ggcatcgtcc gcgagacaaa agacattcag tttttgcagc gccagcgcct
    26161 ctctttcaat ccgcgtttcg ggtagcggtg cgaggctgtc cgtcaggtgg ctaatggcat
    26221 catcaagcag atcattaagc gcttgatgca tttgcgtaat ggcacgattg ggaaaccaca
    26281 gcacgccgat taacgtcatc agcaaggttg gatagaggcc aacaacgata caccacagcg
    26341 ttaagcgcac gactacttcc ggatagtcga gcatggcggg gaaggtttgc ccgtaaatgg
    26401 cgacaatggc gacagcgaaa aagaccagtc ccagccgatg ggtgcgcatc aaaaacatgc
    26461 agcccatcag gatcggcccg gcgatgatca atcggatcaa cggttcgccg tatgaccatt
    26521 tgtagatcaa aaacaggctg ccgatctcca gcaccgtggc aaccacaaac aagatcgcca
    26581 caaatttggt gtaaaacgcg ttcgactgaa taccgtaaaa cagcactgcc agcgataacg
    26641 ccacaaaagg gatctcaaaa gtcatcgaga tcagaatcac cagcaggcag cctacccaga
    26701 gttgcacgat ctgcggcacg cggccgggtc gccgctcgct taactcttca tgaaagaacg
    26761 ccagcagcct gaccaccggt aatggcaggg agttgagcgc gctcattatt gcggctcaag
    26821 attagcgact gccgaagcgc cgatgcggaa catttccggg tcaggtttat cgaccatgat
    26881 tttgaccgga aaacgctggg caacgcggac ccagttaata gaacgagaca cttttggcag
    26941 gccgcccagc accaggccgc cgtcatccgg tagcacgccg tagccaatcg aatccacttt
    27001 accctcgaag gttttgccgc tgtcactcat caggcgaatc gttgcgggtg tacctgagcg
    27061 aatatttttc agatcggttt cgcggaagtt ggcgatcacg taccagtgac gagtgtcgat
    27121 gagggtaaaa atagggcgca tggcagaagc aaattgcccg acggaggttt tgagggaaat
    27181 gatccggcca tcaaatggcg cgcgaacggt cgtcatttcc agatgtagtt tggtcagggc
    27241 aatatccgct tctgcggcgc gctgcgccgt tcttgcacgg tcaacgtctt ctgctgagac
    27301 aaaaccctct ttcagtaatg gctcggtgcg gcgtaatgta tctgtggcct gtttcgcggc
    27361 ggcacgggct ttttctaccg tggcattaac cgagtcggca ccaaactgtt gggcgtcaac
    27421 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cggctttcgc
    27481 caggttggct tcgtacgggc gcgggtcgat gcggaacagc aaatcgccct gtttcaccgc
    27541 ctggttgtcg gtgaccgcca gttctacaat gcgaccgctg acttccggca ccacatcaat
    27601 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    27661 gaaaacaagg gcaaccagcg ccaacgcaac cactaacaga gcagggaatt tactgcgagg
    27721 tgcttttttc ggcgtacttt ccatgagcgc gtctcaaata gattagaaaa atgccagcca
    27781 gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa atgccagatt
    27841 gatatttgcc cgctgaataa ctcgcctcgt gatgagcgtc aaaataaggc tggcaatggc
    27901 gcagaaaaac cagctgggat aataagcgcc gatcaccggg attgccggag ataatgagca
    27961 tcccgaaacc atgaagacgg gaattatcca ggctgttttt ttgaggtgta tcgccatgcg
    28021 agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa
    28081 tatcaatatc cattgaaggc gtcagtatgg ctattttctc gatgaataaa aatagcttta
    28141 tccagccacc gcattcataa cttatatatt ttatttatta attggctgta tatattttta
    28201 aaataaatgc ttttgggggc ttcagcgccc ccaaaagcga gtgacattaa tttggggtta
    28261 cgatattcac ccacaaatcg aaattatcca gacaggcgat aatttcttcc agtttcgcgc
    28321 cattgccaat aattttggct ctcttcgctt tcaccagatc cgcc
//
LOCUS       DAJTYF010000039        28025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTYF010000039 DAJTYF010000000
VERSION     DAJTYF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.039"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(98..388)
                     /gene="ubiK"
                     /locus_tag="OO590_001857"
     CDS             complement(98..388)
                     /gene="ubiK"
                     /locus_tag="OO590_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6637239.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQSDPETLPPTL"
     regulatory      510..658
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            762..1415
                     /gene="ribB"
                     /locus_tag="OO590_001858"
     CDS             762..1415
                     /gene="ribB"
                     /locus_tag="OO590_001858"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6637240.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1676..1846
                     /locus_tag="OO590_001859"
     CDS             1676..1846
                     /locus_tag="OO590_001859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637241.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1904..2677)
                     /gene="zupT"
                     /locus_tag="OO590_001860"
     CDS             complement(1904..2677)
                     /gene="zupT"
                     /locus_tag="OO590_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6637242.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2820..3608
                     /gene="ygiD"
                     /locus_tag="OO590_001861"
     CDS             2820..3608
                     /gene="ygiD"
                     /locus_tag="OO590_001861"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6637243.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3646..4806)
                     /locus_tag="OO590_001862"
     CDS             complement(3646..4806)
                     /locus_tag="OO590_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6637244.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(4812..5483)
                     /locus_tag="OO590_001863"
     CDS             complement(4812..5483)
                     /locus_tag="OO590_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6637245.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5631..7112)
                     /gene="tolC"
                     /locus_tag="OO590_001864"
     CDS             complement(5631..7112)
                     /gene="tolC"
                     /locus_tag="OO590_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6637246.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQACLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7317..7946
                     /gene="nudF"
                     /locus_tag="OO590_001865"
     CDS             7317..7946
                     /gene="nudF"
                     /locus_tag="OO590_001865"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6637247.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7947..8369
                     /locus_tag="OO590_001866"
                     /pseudo
     CDS             7947..8369
                     /locus_tag="OO590_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
     gene            8394..9221
                     /gene="cpdA"
                     /locus_tag="OO590_001867"
     CDS             8394..9221
                     /gene="cpdA"
                     /locus_tag="OO590_001867"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6637248.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9221..9802
                     /gene="yqiA"
                     /locus_tag="OO590_001868"
     CDS             9221..9802
                     /gene="yqiA"
                     /locus_tag="OO590_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6637249.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9831..11723
                     /gene="parE"
                     /locus_tag="OO590_001869"
     CDS             9831..11723
                     /gene="parE"
                     /locus_tag="OO590_001869"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6637250.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11771..12085)
                     /locus_tag="OO590_001870"
     CDS             complement(11771..12085)
                     /locus_tag="OO590_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6637251.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12116..12697)
                     /gene="mdaB"
                     /locus_tag="OO590_001871"
     CDS             complement(12116..12697)
                     /gene="mdaB"
                     /locus_tag="OO590_001871"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6637252.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            13016..13348
                     /locus_tag="OO590_001872"
     CDS             13016..13348
                     /locus_tag="OO590_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR6637253.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(13394..14743)
                     /gene="qseC"
                     /locus_tag="OO590_001873"
     CDS             complement(13394..14743)
                     /gene="qseC"
                     /locus_tag="OO590_001873"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6637254.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14740..15399)
                     /gene="qseB"
                     /locus_tag="OO590_001874"
     CDS             complement(14740..15399)
                     /gene="qseB"
                     /locus_tag="OO590_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6637255.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15551..15943
                     /gene="ygiW"
                     /locus_tag="OO590_001875"
     CDS             15551..15943
                     /gene="ygiW"
                     /locus_tag="OO590_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6637256.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15996..16478
                     /locus_tag="OO590_001876"
     CDS             15996..16478
                     /locus_tag="OO590_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6637257.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            17024..19282
                     /gene="parC"
                     /locus_tag="OO590_001877"
     CDS             17024..19282
                     /gene="parC"
                     /locus_tag="OO590_001877"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6637258.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            19516..20253
                     /gene="plsC"
                     /locus_tag="OO590_001878"
                     /gene_synonym="parF"
     CDS             19516..20253
                     /gene="plsC"
                     /locus_tag="OO590_001878"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6637259.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            20328..21740
                     /gene="ftsP"
                     /locus_tag="OO590_001879"
     CDS             20328..21740
                     /gene="ftsP"
                     /locus_tag="OO590_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6637260.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21851..24068
                     /locus_tag="OO590_001880"
                     /pseudo
     CDS             21851..24068
                     /locus_tag="OO590_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
     gene            complement(24111..24368)
                     /gene="yqhH"
                     /locus_tag="OO590_001881"
     CDS             complement(24111..24368)
                     /gene="yqhH"
                     /locus_tag="OO590_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6637261.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24419..25345)
                     /locus_tag="OO590_001882"
     CDS             complement(24419..25345)
                     /locus_tag="OO590_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6637262.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNNNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            complement(25545..26372)
                     /gene="dkgA"
                     /locus_tag="OO590_001883"
     CDS             complement(25545..26372)
                     /gene="dkgA"
                     /locus_tag="OO590_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6637263.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            complement(26477..27640)
                     /gene="yqhD"
                     /locus_tag="OO590_001884"
     CDS             complement(26477..27640)
                     /gene="yqhD"
                     /locus_tag="OO590_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6637264.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKAAR"
     gene            27834..>28025
                     /locus_tag="OO590_001885"
     CDS             27834..>28025
                     /locus_tag="OO590_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6637265.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYIN"
BASE COUNT         6569 a         6946 c         7440 g         7070 t
ORIGIN      
        1 gtgatgatac tctgaatggg attaagcgat aatgtaatag aaaacactgt aaattagagg
       61 attaattcat aatttacagt gcattaaaga caaatgttta cagcgttggg gggagagtct
      121 ctggatctga ctgctttttg atctcagtac tgcgattttc cagttcgctg attctttgtt
      181 caagcagcgc cagtttctca cgagtccgta acaggacttg cgtttggacg tcgaactctt
      241 cacggcttac aaggtcgagg cgcgtcagct gagcttgtag ggtttggcgg atttttttct
      301 ccacatcttc cccgaactcc ctgattcctt taggcattga ttcgtgaacc tggcgagcga
      361 tttgctcaat ttttttcggg tcaatcattg tagtttccct gtactgatag gtgttgagta
      421 ccattgtagt gcgataaggg taagtcataa accagaatta tgtgaagcta tgcgttgctg
      481 ccgctaatca ttagagttat agtgaatccg cttattctca gggcggggcg aaattcccca
      541 ccggcggtaa atcaactcag ttgaaagccc gcgagcgctt tgggtgcgaa ctcaaaggac
      601 agcagatccg gtgtaattcc ggggccgacg gttagagtcc ggatgggaga gagtaacgat
      661 tctgtcgggc atggacccgc tcacgttatt ttggctatat gccgccactc ctaagactgc
      721 cctgattctg gtaaccataa ttttagtgag gtttttttac catgaatcag acgctacttt
      781 cctcttttgg tacgcctttc gaacgtgttg aaaatgcact ggctgcactg cgtgaaggac
      841 gcggtgtaat ggtgcttgat gatgaagacc gtgaaaacga aggcgatatg atcttcccgg
      901 cagaaaccat gaccgttgag cagatggcgc tgaccattcg ccacggtagc ggtattgttt
      961 gcctgtgcat cactgaagat cgccgtaaac aacttgatct gccaatgatg gtagaaaata
     1021 acaccagcgc ctatggcacc ggttttaccg tgaccattga agcggctgaa ggtgtgacta
     1081 ccggtgtttc tgccgctgat cgtattacga ccgttcgcgc agcgattgcc gatggcgcaa
     1141 aaccttctga tctgaatcgc ccgggccacg ttttcccact tcgcgcgcag gcaggtggtg
     1201 tactgacgcg tggcggtcat actgaagcga ctattgattt gatgacgctg gcaggcttta
     1261 aaccggctgg tgtgctgtgt gagctgacta atgacgatgg cacgatggcg cgtgcaccag
     1321 agtgcattga gtttgccaat aaacacaata tggcgctcgt gactattgaa gacctggtgg
     1381 cataccgtca ggcacatgag cgtaaagcca gctgaaaacc gctgcttaat tactgcctta
     1441 atcaagaaac cgaagttgta gctggcttcg gtttttattt tgccctgcta tgcaagatgt
     1501 taattccgct tctctttcta tgagaaaatt tcattaatat caggcattct ttttcattat
     1561 gatcaaaatc atataattgt tccgttatta tgcgaccata tgaatatatc cgaaaaagga
     1621 tatgtgaaat ttatgcgcta aatgtttagt attgttaaat attaagggta aaaggatgtt
     1681 tatcgcctgg tactggattg tattgattgc tctggttgtg gtgggttatt tcctgcattt
     1741 gaaacgttat tgtcgggcgt ttcgccagga cagagacgca ctgcttgaag cgcggaacaa
     1801 atacttaaac agtacgagag aagagacagc cgaaaaggta gaataaggct ttcgtgaagc
     1861 tggcagaagt tgtccatgtg ctggacaact tctgatgttg tttttaacca attcccgccg
     1921 tttgtagcag cactaaactg aatcccatca ctgacattcc acatagtacg ccatagctgg
     1981 ggttattatt agggtcaatc tctttggcga gcggcattaa ttcatcaacc gagagcgcca
     2041 ccataattcc tgcaaccgcc gccatgattg ccgccatgac caccggggaa atcatgctac
     2101 cgaggattaa ccacgccagc acaccaccaa gaatttctgc tagtccagaa atccccgccc
     2161 acagaatcgc ggtgcgttta gaccccgttg ccgcataaac cgggcctgcc actgccagac
     2221 cttcagggat attgtgcaac gcgacggcca gtgcgatgcc aaatcccagc tccaggttgc
     2281 tgctcgccgt gacaaaggtg gcaatccctt ccgggaagtt atgcagactg atgccgagag
     2341 tgagcagaat ggctgtgcgc ttgatcgatt ttggcaacag ctgcaccgat ttttgcatta
     2401 aatcctgcgg atgcgcatgc ggcaacatgc ggtccaggcc aaagtagcca agcagaccga
     2461 agataaacat tccataaccc aacaccggcg acattccttc agctgctagt gcggcaggaa
     2521 gcatttccat taatgagatg agcaacatga tccccgccgc aaaacctagc gaaaacgcca
     2581 gtaagcggtt cgagggtttt tgtccgagaa cgccgagaaa cgcgccaata aacgtggctg
     2641 cccccgccag tatggtcaga atgagaggta ctgacatcga caactcctta taaatcttca
     2701 ccttcaggca gatcgtcaaa ataactgatg atcatcatca ttgttatccg ggttcttact
     2761 ccgccgaaca agcgtattgt gaggatatca aaatgacacc tttagttaag gatatcatca
     2821 tgtcttcaac acgtatgcca gcattgtttt taggtcacgg tagtccgatg aacgtgctgg
     2881 aagataattt gtatacccgc agctggcaga agttggggat gacattgcca cgcccgcaag
     2941 cgattgtggt ggtttcggct cactggttta cccgtggaac aggagtgacc gcgatggaga
     3001 cgccgcccac gattcatgac tttggtggct tcccgcaggc gctgtacgat acgcattatc
     3061 ctgctccggg ttcgcctgcg ctggcacagc gtctggttga gctgttagcg ccgatcccgg
     3121 tgacgctgga taaagaagcc tggggctttg accacggctc gtggggcgtg ctgattaaga
     3181 tgtatcctga cgccgatatc cctatggtgc agttgagtat cgacagtagc aaacctgccg
     3241 cctggcattt cgaaatgggg cgcaaactcg cggcgttgcg tgatgaagga ataatgttgg
     3301 tcgccagcgg taacgtggtg cataacctgc gcacagtgaa gtggcacggt gatagttcac
     3361 cgtatccgtg ggcgacgtcg tttaatgagt atgtgaaagc gaatctgacg tggcaagggc
     3421 cggtagagca gcatcctctg gtgaattatc tcgaccatga aggtggcgcg ttatcgaacc
     3481 caacgccaga gcactatctg ccgttgttgt atgtgttagg tgtgtgggat gggcaggagc
     3541 caattaccat tccggtcgat ggtatagaaa tgggcagttt aagtatgctg tcggtgcaga
     3601 taggctaaca attaccggat ggcgagtgcc atccggtatc gtggcttatt caacaaaaat
     3661 atgtggataa aaccgagaca tatcctgggt gatcaacgca cggtcttcac gaatgccaat
     3721 tccggcaggt tgatcgttca ccagccagct accaatcagc atatagctgt cgccgaattt
     3781 cggtaacggg tggaattgct gaacaatcat cccttcttcg ccatacggac cttccgctgc
     3841 ttcaatggtt ttgccgttct caatgatcga cacgtttgcg ccttcacggg agaagatcgg
     3901 tttaaccaca tatttttcca tttgcggatg atcatcttcc gcaaaataag cgggcagcag
     3961 gttcgggtga ttcgggaaca tctcccacag tagcggtaga agtgccttgt tggagataat
     4021 actcttccac gccggttcca gccagcgtac gccagcatct tccagcttgg tggagaacat
     4081 ttcgcgcagc ataaattccc acggataaag cttgaacagg ttagaaatca cctgatcctg
     4141 taaatccgtg aactggcctt tttcgcctaa acctatctcg tcgatgtaga ggaactcggt
     4201 ggcgatttca gcttcggttg cgcagtcctg caaatactga atggttccgc gatcttccac
     4261 cgtgtcgcga cagcaggtga gatgcagcaa ctggaagcca cactgttcac gcagctcaac
     4321 gaagcgatcg atcagttttt cttgcagact gttaaactgg tcgctgccct ccggcaagtt
     4381 accggcgtta agctgatctt ccagccagat ccactgaaag aacgccgcct cgtatagtga
     4441 cgttggcgta tcggcgttat tttccagaag tttaggttcg ccagtgccat cccacgccag
     4501 atcaagacgc gaataaagcg atggctggtg agtcagccat gattgacgca caaaactcca
     4561 ggtgtgtttt ggtatgcgga atttggtcat cagctcatcg ctggcgatca ctttttccac
     4621 cactttcagg cacatctggt gcagttcggc ggtgacttct tccagctttt caacctgggc
     4681 gagggtcaac ttgtagtaag catcttcaca ccagtacggc tcgccgtaca tggtgtgaaa
     4741 attgaaaccg tattcgtggg ctttctcacg ccagtccggg cgctcggtaa tactgactct
     4801 ttccatcggt atcagccacc cattgaacga gaagaggtgc cggttgcgct acgctgcata
     4861 gtgctttgtt tggcaacaga ttcaccaaaa ccgccacggg taacggtagt ggtggtcgcc
     4921 ggttttggtg ccattgccgt cttcggtacg gtcatggtgc ggcctggctg ggctgcgcca
     4981 tagtttttac ccgtcgcgtc ggtatattta ccgtaagccg gactggccgg gtttttcgag
     5041 gagaacagcg gctgctgtgc aaatcccgcg ccgccgccca tcagacgccc catcatgtaa
     5101 ccggccatca gcggcatcca gaaactcccg ctggattgct gagcctgcgc ctggttttct
     5161 ggtgccatgc cagcctgggc tggtgcttgc tgacactgac cttcaccaaa ttcagcaaca
     5221 cagtcttcac gggtggcgta tttcggcgca gtacgttcgg cttctttcag cgcattgttg
     5281 tacgcggtgg tacattcggc gcttttgcct gggtttgcag ctgaacagtc gtcagcattt
     5341 tgatagagag acactgtttc atcactcttt tcacagccag ccagcataaa aacagtggca
     5401 accgcgagag cgactggtgt cagatggcgt gcgctccagt ttttgcggaa cgatgcgtgg
     5461 cgtatggatt ttgtccgttt catttttgtc ttccgggacc agtggtaaat acccatcaga
     5521 atagaggatg gctggtcgaa attgaagcga gaagggggaa gaatgcggca gataacccgt
     5581 atctttacgt tgccttacgt tcagacgggg ccgaagcccc gtcgtcgtca tcagttacgg
     5641 aaagggttat gaccgttact ggtggtagtg cgtgcggatg tttgctgaac gacgggtgcc
     5701 gggctatcag gcgcataacc atcagcaata gcattctgtt ccggcgtttg cggggcaacg
     5761 ttttccggat tagtggaaac cggtttgctc agcgcattgt tcagtgccag caaatcctgc
     5821 tcgttcaacg tacccagggc tgacttaata ttcagctgat taatcaggta gttataacgc
     5881 gcattcgcca gctcttgctt ggcgttgtac agcgtggtgg tcgcatccaa cacatcaaca
     5941 atggtacgcg taccgaccga gtagcccgct tccatcgcgt ctaatgagct ttgcgcggaa
     6001 actacggctt gtttgtaggc gttaatacta ctgatagaag cattaatgtt gttgaaggag
     6061 gaacgtacgg tttgcacgac gctacgatgt gcgctttcca gttgctcact ggcaccaaca
     6121 aagttgtact gtgcctgttt cacctgcgag ttaaccattc cgccctgata aatcggcagc
     6181 gagaagctca ggccaacttt gttctggccc atattgctat cgtcatactg ggtaccagcg
     6241 gcaccacggg ttttcgaacc gctataagag gtgtcagaaa tcccggtaga agccgttaaa
     6301 tccagagtcg gtaagtgacc atcctgcgcc tggcgaattt gctcgcgcgc caggtcctgg
     6361 ctcaagcatg cctgtaacag cgacaggttg cgtttttcgg cttctttcag cagcgcgtta
     6421 accggctgtg gtttgtcggt tttaaagttt tcgacattca gcgccgccag ttccggatag
     6481 tagttaccgg tgatctggcg cagctgctct accgcgttat caaggttatt acgtgcggtc
     6541 acttcgttcg ccagcacggt atcgtactgc gcgcgggcgt tctgcacgtc ggtgatcgct
     6601 accaggccca cgttaaaacg ttgggtggtt tgatctaatt gacggtagat cgcttctttt
     6661 tgtgcctgtg tataggaaag aacgtcaata gcattcaaca cgttgaaata agcggtcgcg
     6721 gtgttgagga tcaaggtttg ctgatcggtc tgatatgtga cgtcctgaat ccctgctgct
     6781 ttttcctgca gcgttaacgc acgccatttc gacatatcaa aaatggattg agttaactgc
     6841 agggacgcac tggtcgcgtt cgagttgatg ccgttcgcgt cgcggtagcc gttgctatag
     6901 gtgtaatctg cacctaaacc tagctgtggc agtaatggac tgcgcgcttc attaattttt
     6961 tcaaaggcag catcacgatc ggcggcagac ttacgcaatt ccgggttact aaggcgtgct
     7021 tgctgataaa cttgcatcag gttctcggcc tggctcaacg aactgaaccc agaaaggctc
     7081 aggccgataa gaatggggag caatttcttc atttgcattc cttgtggtga agcagtattt
     7141 agcgcgatca aactgtaaaa ttattgcgga ttctagcaga agccgctacc gcaaaaggtt
     7201 ggcgttacgt gccatagggc gttaatgtgc ctcaatttaa catatgactg ctcaaacggc
     7261 agtcaaacgt cgctgaaatt cacatttaat tcactattag tgccaggaca ttaacaatgc
     7321 ttaagccaga caacctgccc gttacatttg gcaaaaacga tgtagaaatt attgcacgag
     7381 aaacacttta tcgcggcttt ttttcattag atctttatag atttcgtcat cgtctattca
     7441 acgggcaaat gagtcatgag gtacggcggg aaatttttga gcgcggtcac gccgcagtct
     7501 tgctaccctt tgacccagtg cgtgatgaag ttgtgctgat tgagcagatt cggattgccg
     7561 cgtacgacac cagcgaaacc ccctggctac tggagatggt tgccgggatg attgaagagg
     7621 gtgaaagtgt ggaagatgtt gcccgtcgcg aagcgattga agaggcggga ctgatagtca
     7681 aacggaccaa accggtgtta agtttcctgg caagcccggg gggcaccagt gagcgttcgt
     7741 caattatggt gggcgaagtg gacgccacga ccgcaagcgg tattcatggt ctggctgatg
     7801 aaaacgaaga tattcgcgtt catgtggtaa gccgggaaca ggcataccag tgggtagaag
     7861 aggggaaaat cgacaacgca gcgtcggtca tcgctttgca atggctgcag ctgcatcatc
     7921 aagcgttaaa aaatgagtgg gcataaatga agcgttacac acctgacttt cctgaaatga
     7981 tgcgcctgtg cgagatgaac ttttcataat tgcgccgttt gttaccgcgc aattacgcac
     8041 ccggcgaaac tgtaagctat caggtggcaa acgcacaata tcggctgacg attgtggaat
     8101 cgacccgata cactaccctg gtgacaatag aacagactgc gcccgcgatc agttactgga
     8161 gccttccgtc aatgacggtg cgtctgtatc atgacgcgat ggtggctgaa gtgtgttcaa
     8221 gccagcagat ttttcgcttc aaagcgcggt atgattatcc taataaaaag ttgcatcaac
     8281 gcgacgaaaa gcatcaaatt aaccagtttt tagcggactg gttgcgatac tgtttagcac
     8341 atggagcgat ggcgattccg gtttactagc gtcgtgaaac ctaaggacac catttggaaa
     8401 gcctgttaac ccttcctctg gctggtgagg ccagaggaag gattttacaa attaccgata
     8461 ctcacctgtt tgcacaaaag cacgaagccc tgttaggggt aaacacctgg gaaagctacc
     8521 aggcggtgct ggaggcgatt cgtccacacc agcacgaatt cgacctgatt gtcgcgacag
     8581 gtgatttagc gcaggatcaa tcctctgcgg cctatcagca tttcgctgaa ggcatcgcaa
     8641 gttttcgtgc gccctgcgtc tggctgccgg gcaaccacga tttccagccc gcgatgtaca
     8701 gcgcgttaca ggatgcgggt atctccccgg cgaagcgggt gtttattggt gagcaatggc
     8761 aaatcctgtt gctggatagc caggtgtttg gcgtgccgca cggtgagctg agcgagtttc
     8821 agcttgagtg gctggaacgt aaactggccg atgcgccaga acgccatacg ttgctgctgc
     8881 tgcatcatca tccgctacct gcgggttgta gttggctcga tcaacacagt ctgcgtaacg
     8941 cgggcgaact ggataccgtg ctggcgaagt ttccgcacgt caaatacttg ctgtgcggtc
     9001 atattcatca ggagctggat ctcgactgga acggtcgccg cctgctggca acgccgtcga
     9061 cctgtgtgca gtttaagccg cactgttcca actttacgct ggatactatc gcgcccggct
     9121 ggcgtactct cgagttacat gctgatggca cgctgaccac cgaggtgcat cgcctagcgg
     9181 acacacgttt ccaacctgat accgcttcag aaggctactg atgtctacgc ttctttattt
     9241 acacggtttc aacagctcgc cgcgctctgc aaaagcgagc ttgttaaaaa actggctggc
     9301 ggaacatcac cctgacgttg agatgatcat tccgcagttg ccgccgtatc cttccgacgc
     9361 ggcagaactg ctggaatcca ttgtcctgga acatggcggc gattcgctgg gtattgtcgg
     9421 ttcgtcactg gggggatatt acgccacctg gttgtcgcaa tgttttatgc tgcccgcagt
     9481 ggtggtaaac ccggcggtac gcccgtttga attgctgacg gactatctcg gtcagaacga
     9541 gaacccctac accgggcagc aatatgtgct agagtcacgc catatttacg atctcaaagt
     9601 catgcagatt gacccgctgg aagcgccgga tttgatctgg ctgctgcaac agacgggaga
     9661 tgaagtgctg gattaccgcc aggcggtggc gtactacgct tcctgccgcc agactgtcat
     9721 agaaggcggc aaccacgcat tcacgggctt cgaagattat ttcaacccga tcgtcgattt
     9781 tcttggtctg caccatctct gacgatcaac tcgaattgct aacttaaacc atgacgcaaa
     9841 cttataacgc tgatgccatt gaggtactca ccgggcttga gccggttcgc cgccgtccgg
     9901 ggatgtatac cgataccact cgccctaacc atttggggca agaagttatt gataacagtg
     9961 tcgatgaagc actggcgggt cacgcaaaac gcgtggatgt aatcttacat gctgaccagt
    10021 cgttagaagt gattgacgat gggcgcggga tgccggtgga tattcacccg gaagaaggag
    10081 taccggcggt tgaactgatt ctttgccgtc tgcatgcagg cggtaaattc tctaacaaaa
    10141 attaccagtt ctctggcggc ctgcatggcg tggggatttc ggtagttaac gccttgtcga
    10201 agcgcgtaga agttaacgtg cgccgcgatg gtcaggttta taacatcgcc tttgaaaatg
    10261 gcgaaaaggt gcaggattta caggttgtcg gcacttgcgg taaacgcaat accggtacca
    10321 gcgtgcactt ctggccggat gaaaccttct ttgacagccc gcgtttttct gtttcacgcc
    10381 tgacgcatgt gctgaaggcc aaagcagtac tgtgccccgg cgttgagatt acctttaaag
    10441 atgagatcaa caacaccgaa cagcgctggt gctatcagga cggtctgaat gattacctgg
    10501 cggaagcggt aaacggttta ccgacgctgc cagaaaaacc gtttatcggt aatttcgctg
    10561 gcgatactga agctgtggac tgggcgctac tgtggctgcc ggaaggcggt gaactgctga
    10621 ccgaaagcta cgtcaacctt atcccaacga tgcagggcgg tacccatgtt aatggtctgc
    10681 gtcagggcct gttggacgcg atgcgtgagt tctgtgaata ccgcaacatt ctgccgcgcg
    10741 gcgtgaagct gtcggcggaa gatatctggg atcgctgcgc ctatgttctg tcggttaaaa
    10801 tgcaggatcc gcagtttgcc gggcagacca aagagcgtct ctcttcgcgt cagtgtgcgg
    10861 cattcgtttc gggcgtggtg aaagatgcct tcatcctgtg gctgaaccag aacgttcagg
    10921 cggcggagtt gctggcagag atggcgattt ccagcgccca gcgtcgtatg cgtgcggcta
    10981 aaaaagtggt gcgtaaaaag ctgaccagcg gcccggcgct gcctggcaaa ctggctgatt
    11041 gtaccgcgca ggaccttaac cgtaccgaac tgttcctcgt ggaaggtgac tccgcaggcg
    11101 gatctgccaa gcaggcgcgc gatcgtgaat atcaggcgat catgccgctg aaaggtaaga
    11161 tccttaacac ctgggaagtt tcttccgacg aagtgctggc ctcgcaggaa gtgcatgata
    11221 tttcggtagc gatcggtatc gatcctgaca gcgacgatct gagccagctt cgttatggca
    11281 aaatctgtat cctcgcggat gcggactctg atggtctgca cattgccacg ctgctctgcg
    11341 ctttgttcgt aaaacacttc cgcgcgttgg tgaaacacgg tcacgtttac gtcgcactgc
    11401 caccgctcta ccgtattgat ctcgggaaag aggtttatta cgcgctgacg gaagaagaga
    11461 aagagggcgt acttgagcaa ttaaaacgca agaaaggcaa gccgaacgtc cagcgtttta
    11521 aaggtctggg ggaaatgaac ccgatgcaat tgcgtgaaac cacgcttgat ccgaacactc
    11581 gccgtctggt gcagttgact atcgatgatg aagacgatca gcgtactgac gcgatgatgg
    11641 atatgctgct ggcgaagaaa cgctcggaag atcgccgcaa ctggttgcaa gagaaaggcg
    11701 acatggcgga gattgaggtt taaaggaaag aacattgccc ggtcaggatg gccgggcaaa
    11761 caaggcagga ttaaatccct ggctgcagaa tacggatatt catctccagc acgtcgcctt
    11821 ttacggcttt gctgtacgcc ttcatgtgcg gggtttgcag atgcgcttca aggtgcgcga
    11881 tgctttccca ctgctcaatc atcacgatag aatccggtgc catagactgg aaactcacgc
    11941 cagcagcgca atccaccatt ggcgcatagc cgtggcaacc ttcttctttc agtacggttg
    12001 gaacgatttt agcaaactga tccaataccg cctgacggtg atgttgacca ggacgagtac
    12061 ggatttctgc gattacggta agcatggtta actccttcta aagcctgagc tctagttaac
    12121 caaaaatttc cacaagatgc ttgcgatatt cttcagtata gcggggaaca tcaggcattt
    12181 ttatcacgtc attagcgata aatgtcggca gcggttccat accgaggaat tggtttgctt
    12241 tatggaacgg cagatacaca ccgtcaacgc caacgccgtg gaagaactga tctttttcgg
    12301 tgaaggcttc cattggtgcg ttccaggtta gagaaagcat atattttttg ccctgtacca
    12361 ggccgccgga accgtatttt ttcgacggat ctttgcgggt acgaccatcg ctggcataca
    12421 gagtcccgtg accttcggtg aatacatcat caatatattt tttcactgtc cacggcgcgc
    12481 ccatccacca gcctggcatc tgccagatca ccacatcagc ccagagaaag ttttgtactt
    12541 ccgctttgac attgtagtcg ctgtcggcgc gaacgatgcg gacatcatgc ccgaggtcgc
    12601 gcagtgtgcc atccgcgact tcggtcaggg tgtcgttcag ttgaccattg gagtgggcga
    12661 attttttcgc gccgttgata atcaggatgt tgctcatttt ttaacctcaa ggtgagacgt
    12721 ttgtcgacta tagtacgtca cagagcggta cggtgaaatt agcaaaatgt gcaaagtctt
    12781 ttgcgaattt agcaaaagtc tgatctagct ctgactcacc gatgcaggat aaatattttc
    12841 tgaaaatgac aatactgata gccattcctc ataattctta ctcgtaccgg aataatttac
    12901 acgtttggtt cggttttttt ggggattgaa aatatggctt tgaataagta aaagatgaaa
    12961 atatcaacgt gtcatgttga taattataaa tggattttta tgagcgtact gatatatgtt
    13021 aaagcagaaa attaaaacaa tatttgaagc attgctatat ataatgctta cttattggtt
    13081 aatagattct ttttttgctt tcaataagta tgactggatg cttgagtcgg gaggcaatat
    13141 ttgcagtata ccttccgttt caggcgaaga tcgtatattg caagcaatga ttgcagcatt
    13201 ttttcttctt acgccactta ttattcttat cttaagaaaa ctctttatgc gggaaatgtt
    13261 cgagttttgg ctatatgtat tctcgctcgt tatttgtctg gtttgtggtt ggtggctttt
    13321 ttggggacgg tttatatttt gctattagta aaatctatga gagtcgtttt aacggctctc
    13381 atagacagag aagttaccag cttaccttcg cctcaaatcc accttgttcc gcattcccaa
    13441 actcaacatt catgtcatgc agcttggcga ttcgctggac aatcgatagc ccaagtccac
    13501 tgccggtagc ggtttgtccg ggtgggcgat agaagcgttc gccaattcgc gccagtgcct
    13561 ctggtgtcac accggggccg ttatccctca cgatgaaatt atcagcattc agcgtgacgt
    13621 ctatcacgct gccctgtgga ctgtagcgca cggcgttatc cagcaaattt cgcaccaaca
    13681 aacttagcaa tagcggttgt ccggtgcgtt tgatgccgtg ggcattgagt gtcagtcgca
    13741 cgtcaatttt cgcctgctgc gccgtgtggt aaatatccat caccgacgat tgtaggagat
    13801 cttcaagcgg gatctccgcg acgtcctgaa ggttatccag tgagtccagc cgcgatagcg
    13861 tgagcagttg atcaaccaga cgagtagcgc gatcgatccc ggaatgtaat tggagcagtg
    13921 cttttttccg cgcctgcgga tcatcgtcag agagctgcgc aacttcggtt tgcactttca
    13981 gcgctgttaa cgggctacgt agttcgtgag ctgcatcgga ggtaaagcgt cgttctcgaa
    14041 ccatcatcgc atgtgtgcgg gcgaacagtt gatttagcga ctcaaccagt ggccgcactt
    14101 cgctgggtac gccagtcgcg tttagtggtt tttccgagtc agggtcacgc atacgtagtg
    14161 ccagcgccag tttgttcagc ggcgcgagtt cacgacccag tagtaccatc atgatgatta
    14221 acataatcgg cagtgcgacc agccacggga tcaattgccc ggcaacaatc gccagcgcca
    14281 tgtcttcacg gtattcccat tcctggccaa caacgatgcg atatttgcca tcaggtgagg
    14341 tcatccagac aaaacgccaa ggatcgtctt caccgaccag ttgcccgtca gcaaaacctt
    14401 cccgttgata gctataggga atatcttctc cgttatcgcc atcattaagg accattctgc
    14461 cgtcgtgggt aaagatggca aaggtcagcg catcgtcatc aacatgaccg tgttttaatt
    14521 tatttggcgt ctgtgccatg cgatccgccg cgttgatttc gttgagatcg agcgtactta
    14581 accgtttggc aaacagcatc agttgggtgt cgaacaattc atcgacgtta tccgttgttt
    14641 gtttccaggc gacaaagctg gaaagcagcc aggtcaccga ggccagaatt aaaaagatta
    14701 gcgtcagcct gacgcgcaga ctaagacgtt gggtaaattt catttctcac ctaatgtgta
    14761 gccaatacca tgcacggtac gaataaaatc actaccgagt ttgcgtcgca gatgatgcac
    14821 atgcacttca acggcattac tggtgacctc ttcgtcccag gtatacagtt tctcttcaat
    14881 cagtttgcgc ggcagtaccc gaccagcgtt acgcatcagt aattccagca gggcaaattc
    14941 ttttggtttc agtgttaagg gttcgccagc cagcgtggcg atacgtttgc cggggtcgag
    15001 catgacgtta ccgtggcgca gctcgttgct ggcctggccg ttggttcggc gcatcagagc
    15061 ctccagcctg gcggcgactt ctatcaacgc aaaaggttta cacagataat cgtcagctcc
    15121 cagacgcagc ccttctacac gttccgctaa cgcatcacgc gcggtcaggt tcagtaccgg
    15181 ctcacgctga cctttctctc gccattcgcg caaaatatcg cgaccatcca tgcctggtaa
    15241 ggttaaatcc aggatcaccg catcataagg tgcgctataa agcgcctctt ttccctgacg
    15301 accttgtgta aaccagtcga cactaaaacc cattttacta aggccagttt tgatgccgtc
    15361 accaatcagc atgtcatctt ctatcagtaa aattcgcatt ttttcatccc tgcgataacc
    15421 gtatttgctg ccagtaaacc gtgttgtaac gcgctctgta cagcattaaa ttttattttt
    15481 ttcccttaag aagttgttaa ggactatctt gttaaatgct cggaaacaga cattaaaggg
    15541 agtaataaac atgaaaaaat tcgcagcagt aatcgcagta atggccctgt gcagtgcacc
    15601 ggtgatggca gcagagcagg gcggtttttc tggcccatcg gcaacgcaaa gtcaggccgg
    15661 aggatttcag gggccgaacg gcagcgtaac gactgtagaa agcgtaaaat ccctgcgtga
    15721 cgacacctgg gtaaccctgc gcggcaatat cgttgaacgc atctctgacg atctctacgt
    15781 gtttaaagat gccagcggta ctatcaatgt tgatatcgat cacaaacgct ggaacggtgt
    15841 gacggtgacg ccgaaagata cggttgagat tcagggtgaa gtcgataaag actggaattc
    15901 tgttgaaatt gacgtcaaac agatccgcaa agtaaatccg taattgttac cgctcccggg
    15961 acgcgttccc gggaataatt tcgcagggag gcaaaatgac aaacctgaca ctggatgtaa
    16021 acattatcga tttcccatca atccctgtgg cgatgttgcc gcaccgctgt agccctgagt
    16081 tgctcaacta cagtgtggcg aaatttatca tgtggcgtaa agagacgggg ctttctcctg
    16141 ttaaccaaag ccagactttt ggcgtcgcct gggacgaccc tgccaccacc gcaccggaag
    16201 cgtttcgctt tgatatctgc ggcagcgtta gcgaaccgat tcccgataat cgttatggtg
    16261 tgagcaatgg tgaacttacc ggtggacgtt atgccgtggc ccgccacgtt ggcgagctgg
    16321 acgatatttc acacacgata tggggcatta ttcgccactg gctgcctgca agcggcgaga
    16381 aaatgcgtaa agcaccgatt ctgtttcact acaccaatct tgccgaaggg gtgacagagc
    16441 agcgactgga aacggatgtt tatgtgccgt tggcgtgatg gggaatcttt accggagtgt
    16501 gagccaaatc actcgcgata agtttgagcg aacaatgtca caggcgtatt gttgttgggc
    16561 ttataatttc tccggtaagg aataccatgt atacgcgaaa tttattatcc ggtgcgccgt
    16621 aagccgtctc gggggtatcc atgagcaagc cgcacttatg gcaggaggcg cggggcactg
    16681 gtggagacac agctaaactg ggctgaaccg tatacttaca gtcgactact ttgatgcttt
    16741 aggcttccct ggactcgcat aacctcaact tctcgaatcg cccggagcgg acccccatgc
    16801 cgagtggtgt ggcatgggcg caaccttgtg ctgccctcta tgccgatcaa gtctacagca
    16861 aagagttgta tatcaaggca cattgatgcc tttatcgccg tcggagcacg tccggcggcg
    16921 ctattcacgc tgatctcctg tgactcgacg cggcagataa tgtagtatct ccggcaatat
    16981 tgcccctttg aaggctggcg aataagttga ggaatcagaa ttaatgagcg atatggcaga
    17041 gcgccttgcg ctacatgaat ttacggaaaa cgcctactta aactactcca tgtacgtgat
    17101 catggaccgt gcgttgccgt ttattggtga tggtctgaaa cctgttcagc gccgcattgt
    17161 gtatgcgatg tctgaactgg gcctgaatgc cagagccaaa tttaaaaaat cggcccgtac
    17221 tgtcggtgac gtactgggta aataccatcc gcacggcgat atcgcctgtt atgaagcgat
    17281 ggtcctgatg gcgcagccgt tctcttaccg ttatccgctg gttgatggtc aggggaactg
    17341 gggcgcgccg gacgatccga aatcgttcgc ggcaatgcgt tacaccgaat cccggttgtc
    17401 gaaatattcc gagctgctat tgagcgagct ggggcagggg acggctgact gggtgccaaa
    17461 cttcgacggc actttgcagg agccgaaaat gctacctgcc cgtctgccaa acattttgct
    17521 taacggcacc accggtattg ccgtcggcat ggcgaccgat attccaccgc ataacctgcg
    17581 tgaagtagct caggcggcaa tcgcattaat cgaccagccg aaaaccacgc tcgatcagct
    17641 gctggatatc gtgcaggggc cggattatcc gactgaagcg gaaattatca cttcgcgcgc
    17701 cgagattcgt aaaatctacg agaacggacg tggttcagtg cgtatgcgcg cggtgtggaa
    17761 gaaagaagat ggcgcggtgg ttatcagcgc attaccgcat caggtttcag gtgcgcgcgt
    17821 actggagcaa attgctgcgc aaatgcgcaa caaaaagctg ccgatggttg acgatctgcg
    17881 cgatgaatct gaccacgaga acccgacccg tctggtgatt gtgccgcgtt ccaaccgcgt
    17941 ggatatggat caggtgatga accacctctt cgctaccacc gatctggaaa agagctatcg
    18001 cattaacctc aatatgatcg gtctggatgg tcgtccggcg gtgaaaaacc tgctggaaat
    18061 cctctccgaa tggctggtgt tccgtcgcga taccgtgcgc cgccgactga actatcgtct
    18121 ggagaaagtc ctcaagcgcc tgcatatcct ggaaggtttg ctggtggcgt ttctcaacat
    18181 cgacgaagtg attgagatca ttcgtaacga agatgaaccg aaaccggcgc tgatgtcgcg
    18241 gtttggcctt acggaaaccc aggcggaagc gatcctcgaa ctgaaactgc gtcatcttgc
    18301 caaactggaa gagatgaaga ttcgcggtga gcagagtgag ctggaaaaag agcgcgacca
    18361 gttgcagggc attttggctt ccgagcgtaa aatgaataac ctgctgaaga aagaactgca
    18421 ggcagacgcg caagcctacg gtgacgatcg tcgttcgccg ttgcaggaac gcgaagaagc
    18481 gaaagctatg agcgagcacg acatgctgcc gtctgaacct gtcaccattg tgctgtcgca
    18541 gatgggctgg gtacgcagcg ctaaaggcca tgatatcgac gcgccgggcc tgaattataa
    18601 agcgggtgat agcttcaaag cggcggtgaa aggtaagagt aaccaaccgg tagtgtttgt
    18661 tgattccacc ggtcgtagct atgccatcga cccgattacg ctgccgtcgg cgcgtggtca
    18721 gggcgaacca ctcaccggca aattaacgtt gccgcctggg gcgaccgttg accatatgct
    18781 gatggaaagc gacgatcaga aactgctgat ggcttccgat gcgggttacg gtttcgtctg
    18841 cacctttaac gatctggtgg cgcgtaaccg tgcgggtaag gctttgatca ccttaccgga
    18901 aaatgcccat gttatgccgc cggtggtgat tgaagatgct tccgatatgc tgctggcaat
    18961 cactcaggca ggccgtatgt tgatgttccc ggtaagcgat ctgccgcagc tgtcgaaggg
    19021 caaaggcaac aagattatca acattccatc ggcagaagcc gcacgtggcg aggatggtct
    19081 ggcgcaactg tacgttctgc cgccgcaaag cacgctgacc attcatgttg ggaaacgcaa
    19141 aattaaactg cgtccggaag agctacagaa agtcactggc gaacgtggac gccgcggtac
    19201 gttgatgcgc ggtttgcagc gtatcgatcg tgttgagatc gactctcctc gccgtgccag
    19261 cagcggtgat agcgaagagt aatatgccgg ggagggcgct gtttcctccc gctagcaagg
    19321 aacgccggat gaaacgttaa gtcgtcatcc ggcagcgtat tcaacccccg gaaacgataa
    19381 aaattccctt gaaaccgttg tttattcatg cgttgcgatt aacaatacgc ttttccagag
    19441 agcggctttt aacaatgccc taacctgatt tcaggtgacg tacaatgcca gtctctcaga
    19501 ccttcagagg gtgttatgct atatatcttt cgtcttatta ttaccgtgat ttacagcatc
    19561 ttagtctgtg tattcggctc catttactgc cttttcagcc cgcgtaaccc gaaacatgtg
    19621 gccacctttg ggcatatgtt tggccgtctt gcgccactgt tcggcctgaa agttgagtgc
    19681 cgcaaacctg cagatgctga aagctacggc aatgctatct atatcgctaa ccaccagaac
    19741 aactatgaca tggtgacagc atcgaacatt gtgcaaccgc cgacggtgac ggtaggtaaa
    19801 aagagcttgc tgtggatccc cttcttcggg cagttgtact ggttaaccgg caacttattg
    19861 atcgacagaa acaatcgcac taaagctcac ggcaccattg cggaagtcgt gaatcatttc
    19921 aaaaaacgcc gtatttccat ctggatgttc ccggaaggaa cccgcagccg tggtcgcggc
    19981 ctgctaccgt tcaagactgg agcatttcac gcggcaattg cggcgggcgt cccgattatt
    20041 cccgtgtgcg tctctacaac ttcgaataag attaatctta atcgactgca caacggtctg
    20101 gtgattgtcg aaatgctgcc gccaattgac gtcagtcagt atggcaaaga tcaggttcgt
    20161 gagctggctg cccattgtcg ttcgataatg gaacaaaaaa tcgccgagct cgataaagaa
    20221 gtcgcagaac gcgaagccgc cggaaaagtt taagtcggca atctgtattt ttgcggggaa
    20281 cactttcctg cacggtatta ctttagccag ttttacatgg agcaaatatg tcactcagtc
    20341 ggcgtcagtt cattcaggca tcggggattg cactttgtgc aggcgctgtt cccctgaagg
    20401 ccagcgcagc cgggcaacag caaccgctac ccgttccgcc gctacttgaa tctcgccgtg
    20461 ggcaaccgct gtttatgact gtacaacgtg cgcactggtc atttacgcca gggacacgcg
    20521 cgtcggtctg gggaatcaat ggtcgttacc tggggccgac tatccgcgtc tggaagggcg
    20581 acgatgttaa gcttatttac agcaaccgcc tgacagaaaa tgtctcaatg acggtggccg
    20641 ggctacaggt accaggcccg ctgatgggcg gtccggcacg gatgatgtcg ccaaacgctg
    20701 actgggcacc cgtactgcct attcgccaga acgcagctac tctgtggtat cacgccaata
    20761 ctcccaaccg cacggctcag caggtctata acggccttgc cggaatgtgg ctggtggaag
    20821 atgaagtcag caagtcgctg cctatcccca accattatgg tgtggatgat tttccggtca
    20881 ttatccagga taaacggctg gataactttg gtacgccaga atacaacgaa ccgggaagcg
    20941 gcggctttgt tggtgatacg ctgctggtta acggtgtaca aagcccgtac gttgaagtct
    21001 cgcgtggctg ggtgcgcttg cgactgctga acgcgtcgaa ctctcgtcgc tatcaactac
    21061 agatgagcga tggtcgcccg ttacatgtga tttctggcga tcagggattc ctgcctgctc
    21121 ctgtatcggt gaagcaactt tcgctggcac cgggcgagcg ccgcgagatt ctggtggata
    21181 tgagcaacgg cgatgaagtg tcgatcacct gtggcgaagc ggcgagcatt gttgatcgta
    21241 ttcgcggctt ctttgagcca tccagtattc tggtttctac cttggtgcta acgctgcgcc
    21301 caaccggcct tctgccgctg gtcacagaca gtcttccgat gcgcttgctg ccaactgaaa
    21361 tcatggctgg ttcgccaatt cgcagtcgcg atatcagtct gggtgatgac ccgggtatta
    21421 atggacagct gtgggacgtc aaccgtattg atgtcaccgc gcagcaagga acgtgggaac
    21481 gctggacggt acgcgcggac gagccgcaag cgttccatat tgaaggcgta atgttccaga
    21541 tccgtaacgt gaatggcgcg atgccgttcc cggaagacag aggctggaaa gataccgttt
    21601 gggttgacgg acaagtggag ctgcttgttt atttcggtca gccttcctgg gcgcacttcc
    21661 cgttctactt caacagtcag acgctggaaa tggcggaccg tggctcgatt gggcaactgt
    21721 tggtcaatcc ggtaccgtaa tcttcatact cgcccgagga gggcgagtat ggaatacctt
    21781 tcgatttctc tttcgttccg gcttataatc cccggccttt gattattttt ttatcatttc
    21841 tggaagcaaa atgagctcta tctccctgat ccaaccggat cgcgacctgt tctcctggcc
    21901 gcagtactgg gccgcctgtt ttggaccggc accgtttttg ccgatgtctc gtgaagagat
    21961 ggatcaactt ggctgggata gctgcgacat cattttggtt actggcgacg cgtatgtcga
    22021 tcacccaagc ttcgggatgg cgatttgcgg tcgtatgctg gaagcgcagg gctttcgcgt
    22081 cgggatcatc gcccagccgg actggagcag caaagacgac tttatgcgtc tgggtaaacc
    22141 gaatctgttt tttggtgtta ctgctggcaa catggattcg atgatcaacc gctataccgc
    22201 cgatcgccgt ttacgtcatg atgatgccta cacgccagat aatgtcgcgg gtaagcgacc
    22261 ggatcgcgcc acgctggttt atacccagcg ttgtaaagag gcgtggaaag atgtgccggt
    22321 gatcctcggc ggtattgagg ctagcctgcg ccgtaccgcg cattatgatt actggtccga
    22381 taccgtgcgc cgttccgttc tggtagattc gaaagccgac atgctgatgt ttggtaacgg
    22441 tgaacgtccg ctggtggaag tagcgcatcg tctggcgatg ggcgagccga ttagtgaaat
    22501 ccgcgatgtg cgtaataccg caattatcgt aaaagaggcg ctgcctggct ggagcggtgt
    22561 ggattccacc cgtcttgata ccccagggaa aatcgaccca atcccgcatc cgtatggcga
    22621 agatttgccg tgtgcggata acaaacctgt tgcgccgaaa aagcaggaag cgaaagctgt
    22681 aatcgtgcag ccaccgcgcc agaaaccgtg ggaaaaaacc tacgtattgc tgccttcttt
    22741 cgagaaagtg aagggcgata aagtgctgta cgcccatgct tcgcgcattc tgcaccacga
    22801 aaccaaccca ggctgcgccc gcgcattgat gcaaaaacac ggtgaccgct atgtgtggat
    22861 caacccgcct gctatcccgc tttctaccga agagatggac tgcgtttttg cgctgccgta
    22921 caagcgcgtg ccacatccgg cctatggcaa tgcccgtatt ccggcttacg aaatgatccg
    22981 tttttcggtc aacattatgc gcggctgctt tggcggctgt tctttctgtt ctattaccga
    23041 gcacgaaggg cgcattattc agagccgttc ggaagattcg atcattaatg agatcgaagc
    23101 gatccgcgac accgttccag gttttacggg cgtgatttcc gatctcggtg ggccaactgc
    23161 caacatgtat atgttgcgct gcaaatcgcc acgcgctgaa caaacttgtc gccgtttgtc
    23221 gtgcgtttat ccggatattt gtccgcacat ggacactaac cacgaaccga cgatcaacct
    23281 ctatcgccgt gcgcgtgatc tgaaaggcat taaaaaaatc cttatcgcct caggcgtacg
    23341 ttatgacatc gccgtggaag atccgcgcta tatcaaagag ttggcgaccc atcacgtcgg
    23401 cggttatctg aagattgctc cggaacatac cgaagaaggg ccgttgtcga agatgatgaa
    23461 gccgggcatg ggcagctatg accgctttaa agagctgttc gatacctact cgaaacaggc
    23521 aggtaaagag cagtatctga tcccgtattt catctctgcg caccccggta cgcgtgatga
    23581 agatatggtg aatttggcgc tgtggctgaa aaagcatcgt ttccgtctcg accaggtaca
    23641 gaacttctac ccatcgccgc tggcgaactc aaccaccatg tattacaccg ggaaaaaacc
    23701 gctggcgaag attggttata agagcgaaga cgtcttcgta ccgaagggcg acaaacagcg
    23761 tcgtttgcat aaagcgttgt tgcgttacca cgatccggca aactggccgt taatccgcca
    23821 ggcgctggaa gcgatgggca aaaagcatct gattggcagc ccgcgattgc ttagtgcctg
    23881 cgccaaccat tgaagagatg cgtgaagctc gtcgccagaa ccgcaatacc cgtccggcgt
    23941 tgactaaaca tacgccgatg gcgactcagc gtcagacgcc tgctacggca aaaaaagcgt
    24001 cgtctacgca atctcgtccg gtgaatgctg gtgcgaagaa acgccctaaa gcggcggttg
    24061 gacgttaaaa aaaatccccg gcaactgaca tgctgccggg gattttttta tcattctgcg
    24121 tacatacgca agcagcgcag gcaatcaaaa tagtcctgtg catcaagacg cgtattggct
    24181 ctgttagctt cggatttggc agcatagatt tgtgggcgta gtgctttcat atcctgctca
    24241 agccgggcga ttttggcatt taatgtttgt acattgctcg ccagttgatt gaccttttgc
    24301 tcattgacgc agccactgag caagcaggtt gccagaacaa cagctcccac ggtgaaaatc
    24361 gttttcatat agtgttccct tttatttatt gttccggcta attgttattt aattcagctc
    24421 attgctttaa tctggcggcg gtttttgcct caatcagctt aagtaggctg ccgctgttcg
    24481 ggcggataaa atcagcgatt tcccacttac cttcgcgccg ctgtaaaacc agctcttgct
    24541 cttcattgtt gccattggtc agcgtaaaac gaacatgaat gcgggcggaa tcggcggttt
    24601 tctccaggac tcgaatttca tcaacgcgtg aaaatagcga ctcttcgcca aaacagatcg
    24661 gattgtcgta aataaattgc cgttctattt gcatatcttc atcgggatta ttggccgtct
    24721 tgaaggtggt tttatagaag gcattaaccg cctggcggta agagtcggaa accacccacg
    24781 tccacggcca gctataatca gcaatatgtt caaagagttc agcaacaaag gattctggct
    24841 gttctttctg caacgaagct aaagcggcca gcgttttttc attctcgcta ttaactgctt
    24901 gtaagacgct gccatgattg ctgacaatat catcaatgac ccaacgacca ttttccgcca
    24961 ccattttcag tgtctgtgtg gtcttttctt tatcatcttt aaagatacga aagcgcacaa
    25021 cggcatcggc atgatcgacg tcagtttggg ttatcgctac gctttctaac accagatcgc
    25081 caaaatcctg acaatcacaa accggatcat aatccagcca gccaatattg cccggtagtg
    25141 taagattgtt gttcagggta agcgcctgtt gaatacgcgc tgaagttatc gcccgctctc
    25201 cggtttcacc gaaataaggc gtactggcat ctgatttata gttctgataa atctgccgga
    25261 ctgtttgttc tacggtctgt gaaggaggct gcgcgtgtac ggtaaaactt gccagggctg
    25321 ctaaaaacag aagtattatt ttcatctgtt tttcacctct ccgttgcgtt tatcccggat
    25381 atagatgagt tgttctttta agagcttccg gctctgcatg atgatgtcct tatatttggc
    25441 attcctgaac aaggaagagt aacaacgggc gggacgcgag gggaataaat gatttctgaa
    25501 aagtccggta gcggaacatt accgccaccg ggagaatttg cacgttagcc gccgaactgg
    25561 tcaggatcgg gaccgagacg cttgccctga tcgagttttg caattttgcc gagttcgtct
    25621 ttgtcgagac ggaaatccca gacatcaaag ttttcggcaa tacgtgaagg tgtgaccgat
    25681 ttcgggatca ccaccaggcc gctatccaga tgccagcgga taacaatctg cgccggggtt
    25741 ttgccgtatt tatctgccag atcgcgaatg actttctgat cgaaaacgcc ttttcctcct
    25801 tgcgctaatg ggctccagga ttcggtctgg attttgtgtg tcgcgttcca ggcgtgtagc
    25861 tggcgttgtt gcatcagcgg atgaagttcg atctggttta tcacaggcgt cacgccagtt
    25921 tcatcaatca ggcgttgcag gtgatggatc tggaagttgc acacgccgat gcttttgatt
    25981 aatccctctt tttgcaattc gatcatgcct ttccatgctt cgacataatg gtcgatagcg
    26041 ggaacgggcc agtgcattaa gtagaggtcg atataatcaa gctggagttt tttcaggctg
    26101 tcgagcaggg cttcgcgggg gcacttgtgg tcgtcgttcc acagcttagt ggtgatgaac
    26161 agttcttctc tgttgactga ggcatttttc agggctttgc cgacaccttc ttcgttcttg
    26221 taggccgcgg cggtatcaat cgagcgataa cccacttcta acgctttttg aatggcggtg
    26281 attacttcct cattacttgc ttgccagacg cccagtccca gctggggcat gacattgcca
    26341 tcctgtagct taataacggt tggattagcc atacgttcct cctttatatg aactcaccgg
    26401 agcaggctcc ggtgaggtgt tgtgaactta agtctggacg aaatgcccga aaacgaaagt
    26461 ttgaggcgta aaaagcttag cgggcggctt tgtatatacg gcggctgaca tccaacgtaa
    26521 tgtcatgatt ttcgcccagt tgggtcatgc cgtgctcttc cagttttttc agcaaagccg
    26581 ggatggagct gccgtccaga ccgtagtcgg agaggtgggt cggcacgcct aattgctcaa
    26641 agaaattgcg ggttgcggca atcgcggcgt caatacgctc atcgtcggaa ccttcagtga
    26701 tgttccagac gcgttcagca tattgcagca gcttagcgcg cttggtctcg cgtttttcat
    26761 tccacagtgc aggcaggacg atagccagtg tttgcgcgtg atccagaccg tgcatcgcag
    26821 tcagttcgtg gcccagcata tgcgttgccc agtcctgcgg tacgccagcg ccaatcaaac
    26881 cgttcagcgc ctgagtcgcc gcccacatga cgttggcgcg cacatcgtag ttttctggct
    26941 ctttcagggc tttcggacca tcttcgatta gcgtcagcaa aatgccttct gcgaaacggt
    27001 cctgaatttt ggcatcaacc ggtttggtaa catactgttc cacggtgtgt acaaaggcgt
    27061 ccactacgcc gttagccacc tgacgcggcg gcagggtgta ggtataaacc ggatcgagca
    27121 cggcaaatac cggctgaaca tgtgcagaat ggaacgcctg cttgtcgcct gtggttttac
    27181 gggagatcac cgcgcctgcg ttggattctg aaccggttgc tggcagcgtc agcacacagc
    27241 ccatcgggat ggcgctttta atctctttac cgcccgtttg cagaatgtgc cacggatcga
    27301 tattttccgg atagttagcc gctgcggcga taaatttggt gccgtccagt acagaaccgc
    27361 cgccaaccgc cagcaggaaa gtcactttct gttcgcgaac cagtttcacg gcgttcatca
    27421 gcgtttcata agccgggttt ggctcaatac cgccaaattc cagcacgtcc atgcctttca
    27481 gggcatccag aacttgatcg agaacgccgg tttttttcac gctgccgcca ccgtaggtaa
    27541 tcaatacgcg agcatcgtga ggaatttgtt cgcgtaaacc agcgattgcg cctttaccaa
    27601 acagaatgcg ggttggggtg tgcagattaa agttgttcat tacttgctcc ctttgctggg
    27661 ccaatatgag ggcagagaac gatctgcctg atgtttttca ttgtgatcgc cagcgcccta
    27721 gctctcaatg ctcatttctg ccaatgtctt gcctatttct ccagagtgct ggagaaatgc
    27781 tacaaaattg cgcacaatca aattgccgca ttattcctaa gaaattacgc gatatgaaac
    27841 gtgaagagat ttgtcgcttg ctggcggata aagttaataa actgaaaaat aaagaaaata
    27901 gtttgtcaga actgttgccc gatgtgcgtt tgttgtatgg cgagacgcct ttcgcacgta
    27961 caccggtgat gtacgagcct ggcatcataa ttctcttttc cgggcataaa atcggttata
    28021 tcaat
//
LOCUS       DAJTYF010000040        27536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTYF010000040 DAJTYF010000000
VERSION     DAJTYF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.040"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO590_001886"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO590_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6637266.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO590_001887"
     CDS             complement(1614..1937)
                     /locus_tag="OO590_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6637267.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO590_001888"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO590_001888"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6637268.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO590_001889"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO590_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6637269.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLHDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO590_001890"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO590_001890"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6637270.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO590_001891"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO590_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6637271.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO590_001892"
     CDS             7537..8574
                     /locus_tag="OO590_001892"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6637272.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO590_001893"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO590_001893"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6637273.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO590_001894"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO590_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6637274.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO590_001895"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO590_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6637275.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO590_001896"
     CDS             10745..11626
                     /locus_tag="OO590_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6637276.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO590_001897"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO590_001897"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6637277.1"
                     /translation="MTVTTFSELELDKSLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO590_001898"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO590_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6637278.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO590_001899"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO590_001899"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCR6637279.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO590_001900"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO590_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6637280.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO590_001901"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO590_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6637281.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO590_001902"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO590_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6637282.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO590_001903"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO590_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6637283.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTLTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO590_001904"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO590_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6637284.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO590_001905"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO590_001905"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6637285.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO590_001906"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO590_001906"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6637286.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO590_001907"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO590_001907"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6637287.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO590_001908"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO590_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6637288.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO590_001909"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO590_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6637289.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO590_001910"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO590_001910"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6637290.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO590_001911"
     CDS             24893..25273
                     /locus_tag="OO590_001911"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6637291.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO590_001912"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO590_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6637292.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO590_001913"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO590_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6637293.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..>27536
                     /locus_tag="OO590_001914"
     CDS             26442..>27536
                     /locus_tag="OO590_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR6637294.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAV"
BASE COUNT         6793 a         6856 c         7221 g         6666 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tatttaccga aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgtgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattaa aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggcttttg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggaaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatctact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatgc tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagttcc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgctaac ttggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgaggg cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtacgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcttg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatac aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac gactatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cattaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttcacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggagtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgctatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgacct caaagccaaa gtagcccggc agaagctgag cccggacagt ttcacaacct
    26881 acggggtgcg tatcgaccgg attaaagcaa cgttcggcga gcgtgaggtg ttcagcgacg
    26941 taacatacaa tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata
    27001 acgccctgga gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt
    27061 gcaacggccg caatgttgcc gccagtctgc cagatgatta tgtttccttc cgcgtacaga
    27121 aacctacccc cacacggctt tttaccgata ttgaatcaat cgcccgactc tggctcaatg
    27181 ttgctgcctg cacctctgta caccagaaga atgctgttcg cttcatgatc atcactggtg
    27241 ttcgtccgat taatgtccat aacctgcgct gggactacgt ttacgaggag gctggtgaaa
    27301 ttgtttatcc ggaaggggtt atcggcatgc gaggggctat gaaaacacaa aaggctttcc
    27361 gcctgccgat aacgcctgag atccggcgga ttatcgacga gcagaaagcc tggcgtgatt
    27421 cagttcctga gtgcaacagg gattatgtat ttttgcagcc acgtgatcca atgcagccat
    27481 tttcaaaacg atcactggat aagctggtga aaacatacag cccggacggg gctgta
//
LOCUS       DAJTYF010000041        27535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTYF010000041 DAJTYF010000000
VERSION     DAJTYF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.041"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..614)
                     /locus_tag="OO590_001915"
     CDS             complement(<1..614)
                     /locus_tag="OO590_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6637295.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAG"
     gene            complement(611..1873)
                     /locus_tag="OO590_001916"
                     /pseudo
     CDS             complement(611..1873)
                     /locus_tag="OO590_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            complement(1870..2778)
                     /locus_tag="OO590_001917"
     CDS             complement(1870..2778)
                     /locus_tag="OO590_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6637296.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2974..3741
                     /locus_tag="OO590_001918"
     CDS             2974..3741
                     /locus_tag="OO590_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6637297.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(3792..4444)
                     /gene="pphB"
                     /locus_tag="OO590_001919"
                     /pseudo
     CDS             complement(3792..4444)
                     /gene="pphB"
                     /locus_tag="OO590_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(4550..7111)
                     /gene="mutS"
                     /locus_tag="OO590_001920"
     CDS             complement(4550..7111)
                     /gene="mutS"
                     /locus_tag="OO590_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6637298.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLLRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            7187..7366
                     /locus_tag="OO590_001921"
     CDS             7187..7366
                     /locus_tag="OO590_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637299.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            7398..7751
                     /locus_tag="OO590_001922"
     CDS             7398..7751
                     /locus_tag="OO590_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6637300.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(7788..9866)
                     /gene="flhA"
                     /locus_tag="OO590_001923"
     CDS             complement(7788..9866)
                     /gene="flhA"
                     /locus_tag="OO590_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6637301.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(9940..10950)
                     /gene="hypE"
                     /locus_tag="OO590_001924"
     CDS             complement(9940..10950)
                     /gene="hypE"
                     /locus_tag="OO590_001924"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6637302.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(10947..12068)
                     /gene="hypD"
                     /locus_tag="OO590_001925"
     CDS             complement(10947..12068)
                     /gene="hypD"
                     /locus_tag="OO590_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6637303.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(12068..12340)
                     /gene="hypC"
                     /locus_tag="OO590_001926"
     CDS             complement(12068..12340)
                     /gene="hypC"
                     /locus_tag="OO590_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6637304.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(12331..13203)
                     /gene="hypB"
                     /locus_tag="OO590_001927"
     CDS             complement(12331..13203)
                     /gene="hypB"
                     /locus_tag="OO590_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6637305.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(13207..13557)
                     /gene="hypA"
                     /locus_tag="OO590_001928"
     CDS             complement(13207..13557)
                     /gene="hypA"
                     /locus_tag="OO590_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6637306.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            13769..14230
                     /gene="hycA"
                     /locus_tag="OO590_001929"
     CDS             13769..14230
                     /gene="hycA"
                     /locus_tag="OO590_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6637307.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            14355..14966
                     /gene="hycB"
                     /locus_tag="OO590_001930"
     CDS             14355..14966
                     /gene="hycB"
                     /locus_tag="OO590_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6637308.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPACVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            14963..16815
                     /gene="hycC"
                     /locus_tag="OO590_001931"
                     /pseudo
     CDS             14963..16815
                     /gene="hycC"
                     /locus_tag="OO590_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            16818..17714
                     /gene="hycD"
                     /locus_tag="OO590_001932"
     CDS             16818..17714
                     /gene="hycD"
                     /locus_tag="OO590_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6637309.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            17732..19417
                     /gene="hycE"
                     /locus_tag="OO590_001933"
     CDS             17732..19417
                     /gene="hycE"
                     /locus_tag="OO590_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6637310.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTAFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            19427..19969
                     /gene="hycF"
                     /locus_tag="OO590_001934"
     CDS             19427..19969
                     /gene="hycF"
                     /locus_tag="OO590_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR6637311.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KENFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            19969..20736
                     /gene="hycG"
                     /locus_tag="OO590_001935"
     CDS             19969..20736
                     /gene="hycG"
                     /locus_tag="OO590_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR6637312.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            20733..21143
                     /gene="hycH"
                     /locus_tag="OO590_001936"
     CDS             20733..21143
                     /gene="hycH"
                     /locus_tag="OO590_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR6637313.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            21136..21606
                     /gene="hycI"
                     /locus_tag="OO590_001937"
     CDS             21136..21606
                     /gene="hycI"
                     /locus_tag="OO590_001937"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR6637314.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            21685..22617
                     /locus_tag="OO590_001938"
     CDS             21685..22617
                     /locus_tag="OO590_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637315.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEGGTRTDFLQSGYEDFGGNYIAHKCLHIVNL
                     DLMSYSFRTHKDRTRRCGCLLTIRKLKLKNAHHF"
     gene            22601..22912
                     /locus_tag="OO590_001939"
     CDS             22601..22912
                     /locus_tag="OO590_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCR6637316.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            22909..23328
                     /locus_tag="OO590_001940"
     CDS             22909..23328
                     /locus_tag="OO590_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6637317.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(23443..24867)
                     /gene="ascB"
                     /locus_tag="OO590_001941"
     CDS             complement(23443..24867)
                     /gene="ascB"
                     /locus_tag="OO590_001941"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR6637318.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAIGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24876..26324)
                     /gene="ascF"
                     /locus_tag="OO590_001942"
     CDS             complement(24876..26324)
                     /gene="ascF"
                     /locus_tag="OO590_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR6637319.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQPTVAKEVSLN"
     gene            26584..>27535
                     /gene="ascG"
                     /locus_tag="OO590_001943"
     CDS             26584..>27535
                     /gene="ascG"
                     /locus_tag="OO590_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6637320.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSP"
BASE COUNT         6299 a         7287 c         7426 g         6523 t
ORIGIN      
        1 cctgcggtca gataacggat cgggcggtca ccgagaataa aaatcagctt tgccgtttcc
       61 tccagctctt ccatattgtt ggcggcttct tgcaggcttt caccgcaaac cactgggcca
      121 tgatttgcca gtaaaaaagc ctgattgtct gctgccagtt ccgccagatc ctgtgcgatg
      181 cgtttatcgc ccggtcggta atagggcacc agcgggacat ttcccatccg catcaccacg
      241 tatggtgtga acggacgaat aacgttgctg ctgtccagcc cttgcaggca ggaaagcgcc
      301 gtcgaccatg tgctgtgcaa atgcaccacc gctttacagc gcggattgtt gcgatacagc
      361 gccagatgaa agagcacctc tttcgagggt ttgtcaccac ttaaccattc gccatccgcg
      421 gcgactttgg aaagccgctg cggatcgaga ttgcccaggc atgaacctgt cggtgtcgcc
      481 agtaaattcc cgtcaggtaa aagcagcgac agattgccag ccgaaccggt tgcatagccg
      541 cgctgaaaga atgaactggc aatccgcgtc atctcctctc gcaaagactg ctctactttt
      601 gcgaaatcgc tcatgataaa aactctcttt gggctcgtga aaaaaaggct tcatcaccga
      661 agttgccaga tttaagggcg agtgagacag gcttatccag tgcgtttacc cacggcacgc
      721 cgggggaaat ggttgggcca atatgaaacc cttttatgcc caggctctgt gtgactacgc
      781 cggaggtctc accacctgcg acaataaagc gtgtcatgcc ttccgctgct aaccgcgccg
      841 ctagttgaga aaacagagtt tctactgcct gactggcttt ttgtgcaccg tattgctgtt
      901 gaattgctgc caatgcgtca gtgctggcgg tggcaaaaac cagtggagca agtacacttt
      961 cctggcccag aacccactct gccagttcgt gagcataagc ggccagagtt tcggttgaga
     1021 ggcagcgtgc cacatcaact tcacgggctg gtgcaatttg acggtaatgt gccacctagc
     1081 ggttggtcat ttgagagcat gaaccggaga gcactacgcc gcgcccagcg agcggacgcc
     1141 ctgcttcgcg agcctggtta ccgttttctt gcgcccactg ccgggccagg ccaatcgcca
     1201 gaccagaacc gcccgttacc agtggggcat cgcgcaaggc ttctccctga atttccagat
     1261 ggtgttcggt cagcgcatca agcaccgcgt agcggtagcc ctcttgctgt aagcgagcca
     1321 gctcttgacg aacggcatcc acaccttgtt cgaaaacatg tgccgaaacg acgccgcagc
     1381 gccctgtgga ttgcgcttca accagacggg gaagatagct gttggtcatg ggatttaccg
     1441 ggtgatggcg catcccggat tcggccagca gttgattcat tacgaacaaa tacccctgat
     1501 aaaccgtacg tccgttgacc ggcagggccg gagagaagac cgtaaacggc gtgtcgagag
     1561 catccattaa tgcatcggta accgggccaa tattaccttt cgccgtactg tcgaaagtag
     1621 agctgtattt gaaatagatc tgtttgcaac cttgctgttg caaccagctc agagccgcca
     1681 gcgattgctg tgtggcttca accaccggac aggagcgcgt tttcaggctg atcaccagtg
     1741 cgtcgattgc ttccggcatt ttacctgttg gaacaccgtt aatttgtacc gttggtagac
     1801 cgttttccac cagaaaactg gcgatatccg tcgcgccggt aaaatcatcg gcgataacgc
     1861 caatcttgat catgatttcg ctcccggtag agtgatgcca gagaaaatct tgataactgc
     1921 gctatcgtct tctttcccgt aacccgcgtt actggcgctg gtgaacatat tcaatgctgt
     1981 tgaggccaat ggcagcggga agtgcagggc tttggctgta tcggcaacca gaccaagatc
     2041 cttaacaaaa atatcgacgg ctgaatgcgg ggtgtaatcg ccatccacca catgacgcat
     2101 ccggttttcg aacatccagg aatttccggc ggcattggtc acgacgtcat acatcacatc
     2161 cagcgggatc cccgcacggg ctgcaagtgc catcgcttcg gctccggcag caatatgtac
     2221 gcccgctaac aactggtgaa taatttttac ggtcgaacct agtcccggtt ctgcacctat
     2281 gcgataaact tttccggcaa cggcttccag cacgggtgcc agtcgttcaa aggcaatatc
     2341 gctaccggag gccatgacag tcatttcacc gttagcggct tttactgcac caccagaaac
     2401 tggcgcatcc agcatttcca gatcgaatcc agccagagcg gtagcaattt cttgcgcatc
     2461 agcactagcg atagtggaag aaaccattac tgccgtaccg ggtttcagat gttgtgcaac
     2521 gcctgtttca ccaaacagca cctgtttaac ctgggccgca ttgaccacca gcaccagcag
     2581 agcgtccagt ttttcggcaa acgtcgcggc gttatcagaa accccgcaag cacctgcctc
     2641 tttcaacgta gcgcaggcat tgctgttcag gtctgcgccc caggtagaaa aacctgcgcg
     2701 gacacatgac agtgctgctc ccattcccat tgaccctaag ccaacgatac cgacatgaaa
     2761 ctcagatccc gttttcatct gctctccttg ttaatttaag tgatattttg tttgacattg
     2821 tgaatataag cgctgggaga taacgatatg gtgagctgat tcacataaat taacattttg
     2881 tgttatttta tgtgaactaa gcgttagttg cgccgcgcac gtttcgcagg caaatagcgt
     2941 agaatgtcag caggacaaag gaaggagcaa aagttgatac ccgtagagcg tcgccaaatc
     3001 atccttgaga tggtagctga aaaaggcatt gtcagtattg ctgaactaac ggacagaatt
     3061 aatgtgtcac atatgaccat tcgtcgggat ttacaaaaac tggagcagca gggagccgtt
     3121 gtgctggtgt ccggaggcgt ccagtctccg ggacgcgtgg cgcatgaacc ttctcatcag
     3181 gtaaaaactg cgctggcaat gacgcaaaaa gcggctattg gcaagctggc ggcaagtctt
     3241 gttcagccgg gaagttgtat ctatctggat gcgggaacga ccacgttagc gatagcacag
     3301 catctgattc acatggagtc actgactgtg gtcacaaacg atttcgttat tgcgaactac
     3361 ttgctcgaca acagtaattg cacaattatt cacactggcg gtgcagtgtg tcgggaaaac
     3421 cgttcctgtg tcggggaagc cgctgcgacc atgctgcgca gcctgatgat tgatcaggct
     3481 tttatttctg catcgtcatg gagtgtgcgg gggatttcta cgccagcgga agataaagtc
     3541 acggtgaaac gggcgattgc cagtgctagc cgccagcgag ttttggtctg tgatgcgacg
     3601 aaatatggtc aggtggcgac atggctggcg ttaccattaa gcgagtttga tcagattatt
     3661 acagacgacg gtttgccgga gagtgccagt cgcgcgctgg cgaagcagga tctctctttg
     3721 ctggtagcga aaaatgaata atggcctgca ataacatctg gttactcatg cttcacagaa
     3781 gaagcatgag actaccttat tttataaaat gacagccgcc cacttttcgg cgatccggta
     3841 tcaatataaa tctggttagc gaacgtctga atgttatcaa acatcatatg tccaaatata
     3901 aaataatcag cgccgtttat ttgttgtaac tcgccattaa gcgagttctg cacacgataa
     3961 acaggccaga gtaattcgct ctccgctatt tctttgtcaa agagatattc atcacccgga
     4021 taatctgcat gtgcgatgac atattttatt atgtcgttag tgatttcaat aatatgtgga
     4081 aggtgatgga atttcagcaa cagatctatt gcctcttgtt gcaatcattt aaatcgaaaa
     4141 accagtcacc accgctggca agccacatat tgccatcgcc ggtcgcgaac gcatccagcg
     4201 ccattgcttc gtggttgcct ttaacggaga taaaccaggg ctggtttagc aggcgcagga
     4261 cgtcaagact ctccggccca cgatcaatat tatcgccgac agaaataagt aagtcggttt
     4321 cggggaaaaa agagagttga tgtaagcggg attgtaataa ctgatattca ccatgaatat
     4381 caccaacgac ccatatatgg cgatagtgat gggcattgat tttttgatag cgtgtagatg
     4441 gcatggtttt accctgtaaa ataagctttc ctattataca gggtattttt atttgattcg
     4501 tcagttgtcg ttaatattcc cgatagcaaa agactatcgg gaattgttat tacaccagac
     4561 tcttcaagcg ataaatccac tccagcgcct gacgcggagt cagtgaatcc gggtcgagat
     4621 tttccagtgc ttcgaccgca ggcgaagttt cttctggcac tgacagcaaa gacatctgcg
     4681 taccatccac ttgcgtcgcg gcggcgttcg gcgaaatgct ttccagctca cgcagttttt
     4741 gccgtgcgcg cttaataacc tctttcggta cgcccgccag agcagcaacc gccagaccgt
     4801 agcttttgct cgccgcgccg tcctgcacgc tgtgcataaa ggcgatagtg tcgccgtgct
     4861 ccagcgcatc gagatgtacg ttggcgacgc cttccatttt ctccggtagc tgggtcagct
     4921 cgaaatagtg ggtggcaaac agcgtcaatg ccttaatctt attcgccaga ttttctgcgc
     4981 acgcccacgc cagcgacaga ccatcgtagg tggacgttcc acgcccgatc tcatccatta
     5041 acaccagact gtattcggtg gcgttatgta aaatattggc ggtttcagtc atctccacca
     5101 taaaggttga gcgcccggaa gccaggtcat ctgccgcgcc tacgcgggta aagatgcgat
     5161 cgataggtcc aatctcgact ttttgtgccg gtacatagct gccgatgtag gccatcagcg
     5221 caatcagtgc ggtctggcgc atataggtac ttttaccgcc catgttcggg ccggtaataa
     5281 tcaacatacg gcgctgcggc gacagattca gcgggttagc gataaatggc tcattcagta
     5341 cttgttcaac taccggatgg cgaccttcgg taatgcgaat gcccggttta tcaatgaagg
     5401 tcgggcaggt gtagttcagg gtataggccc gttccgccag gttaaccagc acgtcgagtt
     5461 ccgccagcgc gctcgcgctc tgttgcaacg cttccagatg cggcaacagc aggtcgaaca
     5521 gctcttcata aagctgtttt tccagtgcca gtgctttgcc ttttgaggtg agaactttat
     5581 cttcgtactc ttttagctct ggaatgatgt agcgctcggc gtttttcagc gtctggcgac
     5641 gcatgtagtt gatgggtgcc agatggcttt gcccacggct gatttgaatg tagtagccgt
     5701 gcaccgcatt aaagccaact tttagcgtgt ccaggccggt acgttcacgc tcgcggactt
     5761 ccagacgctc cagataatcg gtcgcgccgt cagccagcgc gcgccactca tccagctctt
     5821 cgttatagcc cgatgcgata acaccaccgt cgcgtaacag caccggcggt gtgtcgatga
     5881 ttgctcgctc cagcagatcg cgcagctcgg caaactctcc catcttctca cgtagcgcct
     5941 gtaccggtgc actatcgaca gtttctaact gcgcacgcag ctccggcagt tgctggaaag
     6001 cgtggcgcat acgggccaga tcgcgtgggc gagcagttcg taaagccaga cgtgccagaa
     6061 tacgttccag gtcgccgacc tgacgcagta ccggctgcaa ctcggcggtg aaatcctgca
     6121 atgcgccaat agtttgctgg cgctcaagca acacgcgagt atcgcgcact ggcatatgca
     6181 gccagcgttt cagcatacga ctacccatcg gcgtgacggt gcagtcgagc acagaagcca
     6241 gcgtattttc cgcaccgccc gccaggttct gagtaatttc caggttacga cgtgtcgcgg
     6301 catccataat gatgctgtcc tgctcacgtt ccatggtgat agaacgaata tgcggcaggg
     6361 tcgtgcgttg ggtatctttc gcatactgca acagacaacc ggcagcacaa agtccgcgcg
     6421 gcgcgttctc gacgccaaaa ccgaccagat cgcgggtgcc aaattgcaga ttcaactgct
     6481 ggcgcgcggt gtcgatttca aactcccaca gcgggcgacg gcgcaggccg cgacggcctt
     6541 caatcagcga catctcggcg aaatcttctg catacagcag ttccgccgga ttagtgcgtt
     6601 gcagctctgc cgccatcgtt tcgcggtcgg ccggttcgct caggcgaaaa cgcccggagc
     6661 tgatatccag cgtcgcgtag ccgaaacctt tgctgtcctg ccagatagcc gccagcaggt
     6721 tgtcctgacg ctcctgtaac agggcttcat cgctgatggt gcctggcgta acgatacgca
     6781 caactttgcg ctcaaccgga cctttgctgg tcgccggatc gccaatttgt tcgcagatgg
     6841 caacggactc tccctgattc accagtttgg cgagatagtt ttccaccgca tggtagggaa
     6901 tccccgccat cgggatcggc tctcccgccg aagcaccgcg tttggtcagt gaaatatcca
     6961 gcagttgcga cgcgcgtttt gcgtcgtcat aaaacagttc ataaaaatca cccatccggt
     7021 aaaacagcag gatctcggga tgctgggctt tcagcttgag atactgctgc atcatgggcg
     7081 tatgggcgtc gaaattttct attgcactca tggggttatg tccggttccc tgatattaaa
     7141 tggggtgtga tggtttttat tttcgttgta atcacataag gcgcacatga taccggagtt
     7201 aatcatttca ggggaagaag cgaacggcga gttcttgatg ctggttccgg gaaagatgct
     7261 gttttgcagc ttttgcgtga tgcttatgaa gcaagtacgc aaaattgtat cgattggcga
     7321 acccttcttc tttagagtca tagtgccctt taagattaag atgtaatata aatgcatctt
     7381 aatgaggtgg aggcagcatg tccggcaagc gtatctctcg tgaaaaactg acgattaaaa
     7441 aaatgatcga tctttatcaa gcgaaatgcc cgcaggcgtc agcggagccg gagcattacg
     7501 aggcgttgtt tgtttacgcg caaaagcggc tggataaatg tgtgttcggc gaagagaaac
     7561 cagcctgtaa gcagtgtccg gtgcactgtt accagcctgc aaagcgtgaa gagatgaaac
     7621 agattatgcg ttgggccggg ccgagaatgc tgtggcgtca tccgatctta accgtgcgtc
     7681 atctcatcga tgacaagcgt ccggtgccag aactgccgga aaaatatcgt ccgaaaaaac
     7741 ctcgtgaata gtatgccgga caggcagatc tgtccggcaa tttgcagtta aatcaatgcc
     7801 gatttatcaa ttcccagccg cttcatccgt gacagcaggg tcgtgcgttt cagccccaga
     7861 cgttgcgcag cgcctttagg cccggcaacc acgctgttgg tttctttcag cacgcgcaca
     7921 atcaactgat attcatcttc gccctcctgg gcgacaaccg ttgcggcagg cggcgtttca
     7981 ggctccggta aagcaatatc tggcaatgac agctgcagca cgttgccgcg tgttagcaat
     8041 actgcgcgct caatgacgtt ttccagttcg cgtacgttac ccggccactc catgttgctc
     8101 aaggtgcgca gcgtctcggc aggaatgctg tcgatattgc gccccagacg acgggcaatt
     8161 ttgaaggtaa aggctttcgc cagcagcgga atatcttccg gacgctcgcg tagtggcggc
     8221 aggtgaatcg ggaatacgtt caggcggtaa tagagatcgc tacggaactc tcggtcggcg
     8281 accatttttt tcagatcgcg gttagtcgcg gcaattaaac gtacgtccgt ctgaatgatt
     8341 ttgttgctgc cgagacgttc aaactcctgt tcctgcaata cacgcagcaa cttcggctgt
     8401 aactccagtg gcatatcgcc cacttcgtcg aggaacagag agcttttatc cgccagttca
     8461 aaacgaccaa tgcgctgggc gctggcaccg gtaaaagccc cacgctcatg accaaacaga
     8521 tcgctttcca gcaatccggc aggcatcgcc gcgcagttca ttttgaccat gcggcgatta
     8581 ttacgcccac tgagattatg gatcgcacgg gcaatcagct ctttacccgt gccagtttca
     8641 ccgaggatca gcacggtact gtcactttgc gccaccattt caacttgttt aagcacgctg
     8701 tacatggctt cgctgcggcc aataatctcg ccaaattcac tatcaacatt gttgagttgc
     8761 tcggtcaggg cgaggttttc atcaaccagc cgttctttca gacgatggat ttcctgatag
     8821 gcgagggcgt tatcgacagc gattgccaca cgttcggcaa tctggcgcag caaattcaga
     8881 ttggtagtgg taaacacttt ctcttcgcat tgcgccagtt tcagcacgcc cagcatggtg
     8941 tcgccagaca tcagcggtaa caggcacaag gtttgaatct ggttgcccca ggtgtcgaac
     9001 aacatgcgtt catagggggc taaatcgtcc cgctcgtaga gattgatcag cagcatctct
     9061 ttacttttga acacgcgttc ggtgagggtt ccggcttcat cgacttcgct ctgttcgtgg
     9121 gcgggatgtt gtttatcaag atagtgagtg gagtagatgt tgagtttgtt tttacggtgg
     9181 ctgcgtaaga caatgctgat atcgtcaatg tcgaaatagt aatggatttc tttggcgact
     9241 tcgctgacca gttcgtccat atccaggcgg gaaagcaccg cgttggtaat ggcaaccagg
     9301 atgcggaagt tatcgcgctc ccggcataac aactcatagt cgacattgtt gacgacgcgg
     9361 ctctggattt gttcggtgac gacagaaacg atctgcgtaa atgtttgcag acgattgaac
     9421 tctttttcgc tccagggacg atcgtcataa cgaataaatt cacagccacc aaaaatatgc
     9481 cctcccgccg ccagtggcat caggcaatag tgaccaaatt tggggtatag cccacctgcg
     9541 gccagctgcg gccaggtttc acaaaattct tcgtaactgc aatgcagcgt atcagggcgc
     9601 gacaaaatgc tgcgtaccgg accgtgtgcc agaacagttt cgtcttcata tttaatgggg
     9661 gtgtcttttt cacgcgacgc gtaataagac gcacgttgag tctgcgcttg ccacaacaca
     9721 atagccgcgt tgtcggcgag cgcagaacgc tttaccagtt gcgaaagagc ctcacacagc
     9781 gaggccagat cgggctgctg caatagtgtc cgagtgatgt cgaacaaccc ttgctgtccg
     9841 agatcactca tcggtgtata tgacattttg ttcttccagg aaagcaccgc aaagcggtgc
     9901 aaaaggtcat tgactaacta taggagattt agaattttat tagcatatac gcggaagcgg
     9961 ttcggcgtgt ggtaaatcga gggttcgttt cacgccatac agaccggcaa gacgaacacc
    10021 tttacgttcc accacttcac caatcagcgc cgcgtctttc cccagtggat gggaatgtaa
    10081 cgctgccagc acttgctctg ccgcgttgcg ttcaacggcg atcaccagtt taccttcgtt
    10141 ggcaaagttc agggcgtcca gtcccagcaa ttcgcaaacg ccgcgcacgg caggtttaac
    10201 cggcagcgct gattcagaaa tttcaatacc acaaccgcag gctgccgcga actcatgaac
    10261 caccgcgttt acaccaccac gggtggcatc acgcagcgct ttcacgccgg gaatgtcacg
    10321 cagcgtctga ataagcggcg tcagcaccgc gcagtcgctg accagttcgc catccagccc
    10381 cagctgctca cgcaggttaa ggatagtcgc cccgtggtcg ccgagtgtac cgctaaccag
    10441 caatacatcg cctgcggtta gcgtctgtgc gccccagtga atattcgccg gaattgcgcc
    10501 catgccagcg gtgttgataa acagtttatc tgccgcgccg cgctgcacca ctttagtatc
    10561 gccagtaacg atggcaatgc ctgccgtgcg ggcggtttct gccatgctgg tcactgcggc
    10621 tttcagtgtc tccatcggca atccttcttc gaggataaag ccacaggaga gatagcgcgg
    10681 aatagcgcca ctgaccgcaa cgtcattcgc ggtgccgcaa atcgccagct tgccgatatt
    10741 accgccagga aagaacagcg ggtcaatgac gtaactgtcg gtggagaacg ccagacggtc
    10801 gccttccgct accagctgcg ccagatcaag acgtgcctga tcttcctgct ctgccagcca
    10861 cgggttggca aaggcttcca taaacaggct gttgattaat tgctgcatcg cctggccgcc
    10921 gctgccgtgg gcgagttgga tattattcac gcttcactct cctgctgacg atactgatac
    10981 cacgcggcgc acgctccttc ggaggagacc atcagtgcac caaatgcggt ttgaggatta
    11041 caggtgttac caaacagcgg gcattgatgc ggcttacatt tgcccgttaa tacctcacca
    11101 caacgcgcgc gcgggtcatc gcagacctgc tgcggtgccg ggcggaaatg tgcttcggca
    11161 tcgaatcgtt gataatccgg cgtcaggtgc acgccagaag attcaatcac gcctaagccg
    11221 cgccattcgc tgtcgccgtt gacacagaac acatcggcaa tcgcctgttg cgccagcagg
    11281 ttaccggcat ccggtaccac ccgacgatac tgattctcta ccttgctgtg ggccgctatt
    11341 ttctgctcca ccagcatgac cacgccttgc agtagatcaa ggggttcgaa accagcgacc
    11401 accagcggac gatgaaaatc gctggcgata aaattatagg catcagtgcc gataaccata
    11461 ctaacgtggc ctggcgcgag gaacgcgtcg ataccgttat ccggctgttc cagcaaactg
    11521 cgcagcgtcg ggataagcgt aatgtgctgg cagaagaagt aaaaattctg cacatcacgc
    11581 gctttcgcct gttgcagagt gatggcggtg gtcggcatgg tggtttcaaa acctaagccg
    11641 aagaacacca ctttgcgggt tggattctcc tgcgccagtt tcaacgcatc catcggcgag
    11701 taaacgatgc gcacatcggc accgcgtgct tttgcctgca acagcgatcc ctgtttccct
    11761 ggtacgcgca tggcgtcgcc aaaggtacag aagatgactt ccggatggct ggcaatctcc
    11821 acgcaggtgt cgattctacc catcggcagt acgcacaccg ggcaacccgg accgtggata
    11881 aactcaacgt tttcaggcag taactggtcg aggccgaatt taaaaatggc gtgggtatga
    11941 ccgccacaca cttccataat ccgcagaggg cgttcggcgg tgtaagagag atgtgaagca
    12001 cgttcgcgca gatgctcaat taactgcatc acctgttccg gcgcgcgata ttcatcaaca
    12061 aaacgcatta tttttcctcg ccatacaaca gcgcaccgac atccggctca acgtcaaaca
    12121 tgttttgtaa ggcgtcgaga gtgtcgcgtg cttcggcttc attaattacg ctcatggcaa
    12181 agccaacgtg aaccagtacc cactggccca cgcgcggctg accgttttca tcgcagctgc
    12241 cgactaacgt taaatcgaca tcgcgctgaa tgccgcagac gtcgactttc gcctggttgc
    12301 cgtcaatggt gcggatctgg ccgggaacgc ctatgcacat cgctgtgtct ccagccagtt
    12361 cagccactgg tccatccctt cgccgctggt ggcggaaata aggatgattt caatttctgg
    12421 attgacttcg cgggcgcagg cgatgcactt ctcaacgtca aagttgagat acggcaacag
    12481 gtcaactttg ttgagcagca tcagcgaggc ggcggcaaac atatgcggat atttcagcgg
    12541 tttgtcttca ccttcggtaa cggaaagcac cgctactttg tgtttttcac cgagatcgaa
    12601 gctggccggg catacgaggt tgccaacgtt ttcgataaac agaataccgt tatcgtccag
    12661 tggcagacgc ggtgcggcgt cggcaatcat ctgtgcgtca agatggcagc ctttaccggt
    12721 gttcacctga atcgctggtg taccggtagc gcgaatgcgt gcggcatcgt tcacggtttg
    12781 ctggtcgcct tcaataaccg cgcacggaac gctgtctttc aggcgcatta aggtttccgt
    12841 cagcagggtg gttttaccgg aaccagggct ggaaaccagg ttgagcacca gttgcttgcg
    12901 ggcagcaaag cgcgcgcggt tgcgttcagc cagacggttg tttttgtcca gcacgtcaat
    12961 ttcgacttcc agcatccgac gctggctcat gcccggtgtg tgagtgcccg cttcgccatg
    13021 accgtaatgc aggtcgcctt cttcagtctg gctgggggta aattcaggcg ctttaatgcc
    13081 ggtgattttc atctgcgggc gtgccgccgg ggcaaatggc gcgctacgaa acgcggaatg
    13141 agggttatgt tcatcaccct cgatatacag gttgccttca ccgcaaccgc atgttgtaca
    13201 catcgctcac tcctggtcta tttctatccg ccgaatctgt aaaccgtcgt ctgccacaat
    13261 ctgtagcatg tcaccatgac actgtggaca gcggcggacg cgctgggtca gtagcgtcac
    13321 atactgttgg catgtttcac accagcattc ggcctcttgt tcttcgaggt gcagtttaca
    13381 accttccgcc acgctgccgc ggcaaaccag atcaaaacaa aaggcaagag agctggtttc
    13441 gacacaagaa aatgcgccaa ttttgagcca gaccccagtt acgcgttttg cgccgtgttt
    13501 tgcggcctgc tgttcgatca attccagtgc ccgttggcag agggttattt cgtgcatatc
    13561 gcctcccatt aactattgcc agctacaagc aataattgcg ccagtgttga ttatccctgc
    13621 ggtgaataat gtcgatgatg tcgaaatgac acgtcgacac ggcgacgaaa ttcatcttta
    13681 gcttaaaaat ctctttaata acaataaatt aaaagttggt acaaaaaatg cttaaagctg
    13741 gcatctctgt taaacgagta acctgacaat gactatttgg gaaataagcg agaaagccga
    13801 ttacatcgca cagcggcatc gtcgcctaca ggaccagtgg cacatctact gcaattcgct
    13861 ggttcagggg atcacgttat cgaaagcgcg cctgcatcac gccatgagct gcgcgccgga
    13921 caaagaactc tgtttcgtcc tttttgaaca ttttcgtatt tacgtcaccc tggcggatgg
    13981 ctttaacagc cacaccatcg agtattacgt cgaaacaaaa gatggcgaag acaaacagcg
    14041 gattgcgcag gcgcaactga gcattgacgg catgattgat ggcaaggtca acatccgcga
    14101 tcgcgaacag gttctggaac actatctcga aaaaatcgct ggcgtttacg acagcttata
    14161 caccgccatt gaaaacaatg tgccggtgaa tttaagccaa ctggtaaagg gacaaagccc
    14221 ggcagcatga gctgaggctt tgcccgtttt gcaggcgtta cgcctgtttg gggatgggcg
    14281 tgtcgatgag tgtcgaaaat gacatttcat cggcatgttt tcgtcaaaaa tgacaatcac
    14341 ctgaggaatg cctggtgaat cgttttgtaa ttgccgactc cacgctctgt atcggctgcc
    14401 acacctgtga ggccgcctgt tcagagacgc atcgccagca cggcctgcaa tcaatgccgc
    14461 gcctgagagt gatgctcaat gaaaaagaat ctgcgccgca gctctgtcac cactgtgaag
    14521 atgcaccctg cgcggtggtc tgcccggtta acgccatcac ccgcgttgat ggggccgtgc
    14581 agttgaatga aagtctgtgc gtaagctgca aactgtgcgg catcgcctgc ccgtttggcg
    14641 caattgaatt ttccggcagc cgtccgctgg atattccggc aaacgccaat accccgaaag
    14701 cgccaccggc accgcctgct ccggcgtgtg tcagcacatt gcttgactgg gtgccaggta
    14761 ttcgcgcgat cgccgtcaaa tgtgaccttt gtagctttga tgaacaaggt ccggcctgtg
    14821 tgcggatgtg cccgactaaa gccctgcatc tggtggataa caccgatatc gcccgcgtca
    14881 gcaaacgtaa gcgtgagctg acttttaaca cggactttgg cgatctcacc ttgtttcagc
    14941 aggctcaaag tggagaggct aaatgagcgc aatttccctg atcaatagcg gcgtggcatg
    15001 gtttgtcgcc gccgctgttc tggcatttct cttttctttt caaaaagcgc taagtggctg
    15061 gatagccgga attggcggcg cggttggtag tctgtatacg gcagccgcgg gcttcactgt
    15121 actgactggc tcggttggcg tgagcggtgc gctgtcgctg gtaagctacg atgtgcaaat
    15181 ctccccgctt aacgcgattt ggctgattac cctcggtctt tgcggtctgt ttgtcagcct
    15241 ctataacatt gactggcatc gccacgcgca ggtgaagtgc aacggcttgc agatcaatat
    15301 gttgatggct gccgccgtct gcgccgtcat cgccagcaac ctcggcatgt tcgtggtaat
    15361 ggccgaagtc atggccttgt gtgcggtgtt cctcaccagc aacagcaaag agggcaaact
    15421 gtggtttgcg ctggggcgtc ttggcactct gctgctggtg attgcttgct ggctgctgtg
    15481 gcagcgttat ggcacgctgg atctgcgcct gctggatatg cgtatgcaac agctgccgct
    15541 cggttccgat atctggctgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
    15601 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
    15661 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
    15721 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
    15781 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
    15841 cctgctggct taccacaccc tggaaaatat cggcattatc ctgctggggc tggggctggg
    15901 gctggcgtaa cgggtatcgc gctcgaacaa ccggcgctga ttgctcttgg cctggtcggt
    15961 ggtctgtacc atctgcttaa ccatagcctg ttcaaaagcg tactgttcct cggggcgggg
    16021 agcgtctggt tccgtaccgg tcatcgcgat atcgaaaaac tcggtggtat tggcaagaaa
    16081 atgccggtta tctccatcgc catgttagtc gggttgatgg caatggctgc gctgccgcca
    16141 ctgaacgggt ttgccgggga atgggttatc tatcaatcct tcttcaaact gagcaatagc
    16201 ggcgcgtttg ttgcccgtct gctggggccg ctgctcgctg tgggtctggc aattaccggt
    16261 gcgctggcgg tgatgtgtat ggcgaaagtc tatggcgtca cgttcctcgg cgcgccgcgt
    16321 accaaagaag ccgaaaacgc cacctgtgcg ccgctcctga tgagcgtaag cgtagtggca
    16381 ctggcgattt gctgcgtaat tggcggtgtt gctgcgccgt ggctgctgcc gatgctctct
    16441 gctgctgtac ctctgcctct ggagcctgct aacaccaccg tttctcaacc aatgatcaca
    16501 ttgctgctga ttgcctgccc gctgctgcca ttcatcatta tggcgatttg caaaggcgac
    16561 cgcctgccgt cacgttcccg cggtgcggcc tgggtgtgcg gctacgacca tgaaaaatca
    16621 atggtgatta ccgctcacgg ttttgccatg ccggttaaac aggcgtttgc gccagtgctg
    16681 aaactgcgca aatggctgaa tccggtgtcg ctggtgccgg gctggcagtg cgaaggtagt
    16741 gcgttgctgt tccgccggat ggcgctggtt gaactggcgg tactggtggt gattattgtt
    16801 tcacgaggag cctgagaatg agtgttttat atccgttaat tcaggcgctg gtgttatttg
    16861 ccgttgcgcc gctgctctcc ggtattaccc gcgtggcgcg cgcccgtctg cataaccgtc
    16921 gcgggccggg cgtgttgcag gagtatcgcg acattatcaa actgctgggc cgccagagcg
    16981 ttggcccgga tgcctctggc tggatgttcc gcctgacgcc gtatgtgatg gtgggcgtca
    17041 tgctgactat cggttctccg ctgccgcaac tgggcgattt gatcacctta ctgtatctct
    17101 tcgccatcgc gcgtttcttc tttgccattt ccggtctgga taccggtagc ccgtttaccg
    17161 ctatcggtgc gagccgtgaa gcgatgcttg gcgtgctggt cgaaccgatg ttgctgcttg
    17221 gtctgtgggt tgccgcacag gttgccggtt ccaccaacat cagcaacatt accgacaccg
    17281 tttatcactg gccgctgagc caaagcatcc cgctggtgct ggcgctctgc gcctgtgcgt
    17341 tcgccacttt tatcgaaatg ggcaaactgc cgttcgacct ggcggaagcc gagcaggagt
    17401 tgcaggaagg tccgctctcc gaatacagcg gtagcggctt tggcgtcatg aaatggggta
    17461 tcagcctgaa acagctggtg gtgttgcaga tgttcgtcgg ggtgtttatt ccgtggggac
    17521 aaatggaaac cttcaccgtc ggtggactgc tgctggcgct ggtgattgcc atcgtcaaac
    17581 tggtggtcgg cgtcctggtt atcgcgctgt tcgaaaacag tatggcccgt ctgcgtcttg
    17641 atattactcc gcgcattacc tgggctgggt ttggctttgc atttttagcg ttcgtctcct
    17701 tgctggcggc gtgattaaag agagtttgag catgtctgaa gaaaaattag gtcaacatta
    17761 tctcgccgcg ctgaatgagg catttccagg cgtcgtgctg gaccacgcct ggcagaccaa
    17821 agatcagctg actgtcaccg taaaggtgaa ctatctgccg gaagtggtgg agtttctcta
    17881 ctacaagcag ggtggctggc tgtcggtgct gtttggtaac gacgaacgca aactgaatgg
    17941 tcattacgcc gtttattacg tgctgtcgat ggagaagggc accaagtgtt gggtaacggt
    18001 tcgcgtcgaa gttgacgcca acaaaccgga gtatccgtcc gtgacgccgc gcgttccggc
    18061 ggcggtgtgg ggcgagcgcg aagtgcgcga tatgtacggt ttgattccgg ttggtctgcc
    18121 ggatgactgg ccggatgaac tttatccgct gcgtaaagac agcatggatt atcgtcagcg
    18181 tccggcaccg actaccgatg ctgaaaccta cgagttcatc aacgaactgg gcgacaagaa
    18241 aaacaacgtc gtgccgattg gtccgctgca cgtcacttct gacgaaccgg gtcacttccg
    18301 tctgttcgtc gatggcgaaa acattatcga cgccgactac cgtctgttct acgtccatcg
    18361 cggtatggaa aaactggcgg aaacccgcat gggttataac gaagtgacct tcctctctga
    18421 ccgtgtgtgc gggatctgcg gctttgccca cagcaccgcc tacaccacgt cggtggaaaa
    18481 cgcgatgggt attcaggtgc cagaacgcgc gcagatgatc cgcgccattc tgctggaggt
    18541 ggaacgcctg cactcgcatc tgctcaacct cggcctcgcc tgtcacttta ccgccttcga
    18601 ctccggcttt atgcagttct tccgcgtgcg tgaaacctcc atgaaaatgg cagagatcct
    18661 taccggtgcg cgtaaaacct acggcctgaa cctgatcggc gggattcgtc acgatctgct
    18721 gaaagatgac atgatccaga cccgccagct ggcgcaacaa atgcgtcgtg aagtgcagga
    18781 gctggtggat gtgctgctga gtacgccgaa catggaacaa cgcaccgtcg gcattggtcg
    18841 tctggacccg gaaatcgccc gcgatttcag taacgttggc ccgatggttc gcgccagcgg
    18901 tctcgcccgc gatactcgcg ccgatcaccc gtttgtcggt tatggcctgc tgccaatgga
    18961 agtccacagc gagcagggct gcgacgttat ttcccgtctg aaagtgcgaa tcaacgaggt
    19021 ctataccgcg ctgaatatga tcgactacgg tctggataac ctgccgggcg gtccgctgat
    19081 ggtggaaggc tttacctaca ttccgcaccg tttcgcgctg ggctttgccg aagcgccgcg
    19141 cggcgacgat atccactgga gcatgaccgg cgacaaccag aagctgtacc gctggcgctg
    19201 ccgtgccgcg acctacgcga actggccgac cctgcgctac atgctgcgtg gcaataccgt
    19261 ttccgatgcg ccgctgatta tcggtagcct ggacccttgc tactcctgta ccgaccgcat
    19321 gaccgtagtc gatgtgcgta agaagaagag caaagtggtg ccgtacaaag aactcgagcg
    19381 ttacagcatt gagcgtaaaa actcgccgct gaaataagga atcgccatgt ttacctttat
    19441 caaaaaagtc atcaaaaccg gcacggcgac ctcgtcttat ccgctggagc cgattgcggt
    19501 tgataaaaac ttccgtggta agccagagca gaacccgcag cagtgcatcg gctgcgcggc
    19561 ttgcgtcaat gcctgcccgt caaacgcctt aacggttgaa actgacctcg ccacaggaga
    19621 gctggcgtgg gagtttaatc tcgggcgctg catcttctgt ggacgctgcg aagaagtttg
    19681 cccgacggcg gcgatcaaac tgtcgcaaga gtacgaactg gcggtgtgga agaaagaaaa
    19741 cttcctgcaa cagtcccgct tcgcgctgtg caactgccgc gtctgcaatc gtcctttcgc
    19801 cgtacagaaa gagatcgact acgccattgc gctgcttaag cacaatggtg acagccgcgc
    19861 ggaaaaccac cgcgaaagct ttgagacttg cccggaatgt aagcgccaga aatgcctggt
    19921 gccgtccgac cgtattgaac tgactcgcca tatgaaagag gccatctgat gagcaattta
    19981 ttaggccccc gtgacgccaa cggcattccg gtcccgatga cggtggatga atccatcgcc
    20041 agcatgaagg cgtcgttact gaaaaaaatc aaacgttctg cctatgttta ccgcgtggac
    20101 tgcggcggct gcaacggctg cgaaatcgaa attttcgcca cgctttctcc gctgttcgat
    20161 gcagaacgct tcggcattaa agtcgttcct tcaccgcgtc atgcggatat tttactgttt
    20221 accggcgcgg tcacccgtgc aatgcgatcc cctgcgctgc gtgcgtggca gtccgcgccg
    20281 gacccgaaaa tctgtatctc atacggtgcc tgcggtaaca gcggcgggat cttacacgat
    20341 ctctactgcg tgtggggcgg tacggataaa attgtccctg tggatgttta tatccctggc
    20401 tgcccgccaa cgcctgccgc cacgctgtac ggctttgcaa tggcgctcgg cctgctggag
    20461 cagaaaattc acgcccgtgg gccgggtgaa ctggatgaac aaccggcgga gatcctgcat
    20521 ggcgatatgg tgcagccgct gcgcgtgaaa gtggatcgcg aagcacgtcg cctggcgggt
    20581 tatcgttacg gtcgtcagat tgccgatgat taccttacac agttagggca gggcgaagaa
    20641 caggttgcac gctggctgga agcggaaaac gatccgcgtc tgaacgagat tgtcagccat
    20701 ctgaatcatg ttgttgaaga ggcgcgtatc cgatgagtga aaaggtggtg ttcagtcaac
    20761 tgagccgtaa atttattgat gagaacgatg ccacgcccgc cgaggcgcag caggtggtct
    20821 attacagcct ggcgattggt caccaccttg gggttatcga ttgcctggaa gcggcgctca
    20881 cctgcccatg ggatgaatat ctggcatgga ttgccactct ggaggcgggc agcgaagccc
    20941 gccacaaaat ggaaggcgta ccgaaatatg gcgagatcgt catcgacatt aaccatgtgc
    21001 cgatgctggc caacgcattc gataaagccc gggcagcgca aacttcgcag cagcaggaat
    21061 ggagtacaat gctgttaagt atgctgcatg atattcatca ggaaaacgcc atctatttga
    21121 tggtgaggag actgcgtgac tgacgtttta ctctgtgttg gcaatagcat gatgggcgat
    21181 gatggcgctg gcccgctgct ggcggaaaag tgcgccgccg cgccgaaagg taactgggtg
    21241 gtgattgacg gcggtagtgc accggaaaac gacatcgtcg ccatccgtga actgcgcccg
    21301 acacgactgc tgattgtcga cgccacggat atggggctaa accccggcga aatccgcatc
    21361 atcgacccgg atgatatcgc cgagatgttt atgatgacta cccataacat gccgttgaat
    21421 taccttatcg accagttaaa agaagacatt ggtgaagtga ttttcctcgg cattcagccg
    21481 gatatcgtcg gcttttacta cccgatgacc cagccgatta aagatgcggt agaaaccgtt
    21541 tatcaacgac tggaaggctg ggaaggaaat ggtggatttg cgcagttagc ggtggaagaa
    21601 gaataattct ccatcaggga attcagggaa ttcagggaat tcagggaatt cagggaattc
    21661 agggaattga acatcagaac agggatgtgc gcttttttat tgagcttagt gctgcctgca
    21721 caggccacca gctttaccga atatctgcca atgagtgaca gcgaatacgc gcaaaagcgg
    21781 gcgctgaaac ctctcctgac catgccttat gatgccgagc aaacctggca ttttaggaaa
    21841 gtgggcgttg ctggcgtgac gcttgagaag atgccaaatg aagaagatta ctggcaatta
    21901 accgctaaag accgcgccgg taaatcctgg actgtgcctg tgggtatgct ggcaaacata
    21961 gcgggcaaag ggcaactcta tcgcgccgat cttgatcgta acggcattca ggatctggtt
    22021 atctggttac caagtaccgg taatggtctg gctccttatg cacatttaat tcttatgacg
    22081 ttcacgcgcg aaggccgtcc ctgtgtcttt gaacccaggg gattctacac cgcaagcaaa
    22141 actggcgtag atgatttgct ggatttgcaa ggcaatggtc acacgcaatt gctggatatg
    22201 cagtttaatt ctggttactg gataaccagt ctgtatcagg taaaagacgc caaatggcag
    22261 cgtgttcacg gctggtttga caagttaagc tatcccgcgc tcacgcgttt tacttatacc
    22321 cccaaccgta aactcgtcct caaacccatc gccgggcgcg acccgcaaac ggaagatctg
    22381 gcgcagacgc agcgttgctt gataaagggg gatgtgcttg aggggggaac cagaactgac
    22441 tttttgcaga gtggctatga ggattttggg ggtaattata tcgcgcacaa gtgtttacat
    22501 attgtgaact tggatttgat gagctatagt ttccgaacac ataaggatag aactcgcaga
    22561 tgtggctgtt tacttactat aaggaagtta aaacttaaaa atgcacatca tttctaaagc
    22621 gccatttgaa gagtctgcgc gtaaatatcc taacgacgct ctggctcttc aggcattgta
    22681 tcgggttatt aaggaaacgg atttttctac gccagaagag atgcgaacgg cgtttcccaa
    22741 cctcgacaat tttaaatatc gcaacaaatg gtacgttctg gatgtaggtg ggaacaacct
    22801 aggggtgatt gcctacataa actttgttaa taagcggttc tttgtgaagc acataactaa
    22861 tcacgccgag tatgacaaac tgacacgtta ctacagagag aacaaagaat gactgctaat
    22921 gctgcacacg ctgttaaagc aacaagagaa ctggtcaatg cagtcccctt tctgggaggt
    22981 agtgactcag aagatgatta tcgtgaagca ctggaactgg tcgaatattt gattgaagaa
    23041 gatgacacca atccacttat cgactttctg gcgagccgca ttgcagaata tgaaaacaac
    23101 aaccaagagt ttgcagagtt cgacaaagcc gttgcggcaa tgcctgttgg cgtggcgtta
    23161 cttcgcacgc tgattgatca acataatctg acctatgcgg atttgaagaa cgaaatcggc
    23221 tctaaatccc tggtcagtca gattttgtct ggacaacgat ccctgacgat atcccacatt
    23281 aaggcacttt cagcccgatt tggcgttaag ccggaatggt ttctttgatt tttgactaat
    23341 gccaaatgct aaattgcccg atgcgctgcg cttatcgggc ctacatgtcg cacgaactat
    23401 gtaggccgga taaggcgttc acgccgcatc cggcactgtt acttactcta aatcttcccc
    23461 attactggca atcacttttt tataccacca gaacgatttc ttacgtgtgc gcgtcagcgt
    23521 gccgttgcct gcgtcgtcac ggtctacgta gacaaaaccg tagcgtttgc tcatttcacc
    23581 cgtagaggcg gaaactaaat caatacagcc ccatgtggtg tagcccatca gcggaatgcc
    23641 atctgcaatc gcttcgccta ttgcgcggat atgttcgcgt aaatagctga tgcgatagtc
    23701 atcgttaatc tcgccattgg cagcaagttc atcttttgcg ccgaggccgt tttccaccag
    23761 aaacagcggc ttctgataac gatcgtacat catattcatg gtgatacgca gaccgagtgg
    23821 atcaattccc cagccccagt cgctcacttg cagatacggg ttacgcagcg atttcaccac
    23881 gttcgccgca ctgctgttgt tggcgttcat ctccgccgag gcgcagcgcg aggcgtaata
    23941 gctgaaagag acaaaatcga cggtgttttt caggatctca tcatcgccca gcgctttatc
    24001 tatggttacc cctttttcgc gaaatacgcg ggcagagtaa gccggatacg cgccgcgcgc
    24061 ctgcacatcg ataaaaaaca ggttttcccg atctttctcc agcgccgccc agacatcttc
    24121 cggtttgcag ctgtaagggt agaagttacc gcccgccagc atacagccca cctgattttg
    24181 cgggttaacc tcatgggcga ttttggtggc tagcgcactg gcaaccagct ggtgatgcgc
    24241 ggcctgatat ttcacctgat cctgattttc accttcttca aacaccagac ccgcgccgga
    24301 gaacgggcta tgcaacataa tgttgatttc attgaaggtt agccagtatt tcaccagacc
    24361 atcaaatgct tcaaagcagg ttctggcgta gcggctgaaa aactccacca gcttgcggtt
    24421 acgccaggag ccatattcgg tgaccagatg catcggcaca tcgaagtggc acaacgtgac
    24481 cagcggttcg ataccgtact ttttacactc ttcaaagaca ggacgataaa aagcaatgcc
    24541 ctgctgattg ggcgtgattt catcgtcctg cggaaagaga cggctccagg caattgaggt
    24601 acggaaaacc ttgaatccca tctctgccat cagggcgata tcttctttat aacgatgata
    24661 aaaatccgtc gcctcatggc tgggataaaa ctcgtcatcg cgcaactgaa aacgtttttc
    24721 cagccccagt ttcaccgcca ttcgatgctc gccgtgtggg atcatatcga cggtggtcag
    24781 cccttttccg ccttcacgga acgcaccttc agactggttg gcggcaagcg cgccgcccca
    24841 taaaaaacct tctggaaata ctgacatttt catcctcaat taagacttac ttctttggcg
    24901 acggtcggtt gatgctttcg cgcctgggca gccgcttcct caacaggaat atcctcaaag
    24961 ccgagcaaca gtgtgaggat aaacgacagc accaccgcca gcgccatgac cgcgaacacc
    25021 cagacgatgc tcattggatt cgccggatcg aagaattgca cgctggtaaa tagccccggc
    25081 gctgccattg agtggctggc aagccccgcc ataccggcaa ccgcgccgca aataaaaccg
    25141 ctgataagac tggcgataag cggacgtttc aggcggatcg ccacgccgta taacgccggt
    25201 tcggaaatcc ccgccataat ggctgatgcc gccgcagcca gcgccgtctg gcgcagttcc
    25261 gggtttttcg ttttccacgg caccgccagt gatgaaccgc ccagcgacag gttagcgccg
    25321 atctctgatg gcatgaccat tccttctttg ccggtttcgg caatggtctg aatgatggtt
    25381 ggcgtaaaga cgcggtgcat cccggtcatt accagcagag gccacagcgc gcccataatg
    25441 gcgactgaaa gccagcccag ataaccatga atggtgtaaa ccagcgccga aatggcgcta
    25501 ccgatccaga taccaatcgg gccaatcagc aggattgcca gcggtgcggc aatcaacaca
    25561 atcagcattg gcttgaggaa gtttttggtc accgccggtg taatgcgatc cacccagcgt
    25621 tcgatatatg acaggcacca ggtcatcacc agcgccggga tcaccgtgta ggtgtatttc
    25681 accgcggtga ccgggatcag ggcaaattcg acatgttcac cctgggccgc tttcgccatt
    25741 aattcaataa aactcggatg taccagcaca cccgcgatgg caatcgccag cgacatattg
    25801 gttttaaatt tgatggcggc agatgccgcg accattagcg gcaggaagaa gaaagcaccg
    25861 tcaccaatca cattcagaat ggttaaggtc ggcgatcctt ttgtcagcac gccgctcatc
    25921 tcgaggatca tcgccagcag tttgaccatc gatccgccga taatcgccgg gatcagcggt
    25981 gacatggtgc cgatcagcgc atcgaggatc cccgcaccaa tgcgacgcag cgtgagtttg
    26041 ggtttaccca cgggctgtgc gggctgcata tctcccggca gcaggctgac gacttcctga
    26101 aaggcctgcg aaaccgtatt gccgataatt acctgacact ggttgtcact acgtaccacg
    26161 ccgagcacgc cggggatggt ttttaacgtc gggctgtcga taagctggtc atctttgata
    26221 acaaagcgca aacgcgtcat acagtgcgtg actgccgaga tgttatcaac gccgcccagt
    26281 gccgctatca ccgagcgtgc cagcgccgca taatttttgg ccatcggatt ttatcctgtt
    26341 atcagtaggg tagatgtagt tgttatcagt cgacgtgacg gaaaaatacc gatgctgaaa
    26401 cggaattaag aaaccggttt cacaaaatca tgaatagttg tcactaaata agcaagagag
    26461 ttaattgttc attttttata tttgtgaaac cggtcacctg aatatttttc ccgccgattg
    26521 cgcaaggaca atcggcaaac ggtgcagtac actgcgttga ttgcgtgtaa agggatgaaa
    26581 atgatgacga cgatgctgga agtggcgaag cgcgccgggg tttcaaaagc gaccgtttcc
    26641 cgcgtgcttt caggtaatgg ctacgtcagc caggagacta aagatcgcgt gtttcaggcg
    26701 gtagaagaga gcagttaccg cccaaatttg ctggcccgca atctgtcggc gaagagtact
    26761 cagacgctgg ggctggtagt gaccaacacg ctttatcacg gcatttattt tagtgaatta
    26821 ctgtttcatg ccgcgcgaat ggcggaagag aaagggcgac agttgctatt ggcagatggt
    26881 aaacacagcg ccgaagaaga gcgccaggcg attcagtatc tgctggattt gcgctgcgac
    26941 gccattatga tttacccgcg ttttttaagc gtggatgaga ttgatgacat cattgacgcg
    27001 cacagtcagc cgataatggt gcttaatcgc cgcctgcgca ataatagcag ccatagcgtc
    27061 tggtgcgatc ataaacagac cagctttaac gccgtggcag agttgataaa cgccgggcat
    27121 caggagattg ctttccttac cggctcgatg gattccccca ccagcattga acgtcttgcc
    27181 gggtataaag acgcgctgtc gcagcatggt attgcgctca atgaaaaact tatcgctaac
    27241 ggtaaatgga cgcccgccag cggggccgaa ggggtagaaa cgttgctcga acgtggggct
    27301 aaatttagcg cgttagttgc cagtaacgac gatatggcga taggtgcgat caaagcgttg
    27361 cacgagcgcg gcgtagcggt gccagagcag gtgtcagtta tcggattcga tgatatcgca
    27421 attgccccct atatcgttcc ggcgctctcc agcgtaaaaa ttccggtgac tgagatgatt
    27481 caggaaatta ttggacggct gatttttatg ctcgatggcg gggatttctc accgc
//
LOCUS       DAJTYF010000042        26966 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTYF010000042 DAJTYF010000000
VERSION     DAJTYF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.042"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..277
                     /locus_tag="OO590_001944"
     CDS             <1..277
                     /locus_tag="OO590_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR6637321.1"
                     /translation="LKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVE
                     TLAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(339..959)
                     /gene="rhtB"
                     /locus_tag="OO590_001945"
     CDS             complement(339..959)
                     /gene="rhtB"
                     /locus_tag="OO590_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6637322.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1070..2092
                     /gene="pldB"
                     /locus_tag="OO590_001946"
     CDS             1070..2092
                     /gene="pldB"
                     /locus_tag="OO590_001946"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6637323.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2100..2900
                     /gene="yigL"
                     /locus_tag="OO590_001947"
     CDS             2100..2900
                     /gene="yigL"
                     /locus_tag="OO590_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6637324.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            2976..3875
                     /gene="bioP"
                     /locus_tag="OO590_001948"
     CDS             2976..3875
                     /gene="bioP"
                     /locus_tag="OO590_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6637325.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3763..4716)
                     /gene="metR"
                     /locus_tag="OO590_001949"
     CDS             complement(3763..4716)
                     /gene="metR"
                     /locus_tag="OO590_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6637326.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            4952..7213
                     /gene="metE"
                     /locus_tag="OO590_001950"
     CDS             4952..7213
                     /gene="metE"
                     /locus_tag="OO590_001950"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6637327.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7252..8067)
                     /locus_tag="OO590_001951"
     CDS             complement(7252..8067)
                     /locus_tag="OO590_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6637328.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8329..9090
                     /gene="udp"
                     /locus_tag="OO590_001952"
     CDS             8329..9090
                     /gene="udp"
                     /locus_tag="OO590_001952"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6637329.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9231..10658
                     /gene="rmuC"
                     /locus_tag="OO590_001953"
     CDS             9231..10658
                     /gene="rmuC"
                     /locus_tag="OO590_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6637330.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFCRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSC"
     gene            10753..11508
                     /gene="ubiE"
                     /locus_tag="OO590_001954"
     CDS             10753..11508
                     /gene="ubiE"
                     /locus_tag="OO590_001954"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6637331.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11522..12127
                     /gene="ubiJ"
                     /locus_tag="OO590_001955"
     CDS             11522..12127
                     /gene="ubiJ"
                     /locus_tag="OO590_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6637332.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGIRKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12124..13764
                     /gene="ubiB"
                     /locus_tag="OO590_001956"
     CDS             12124..13764
                     /gene="ubiB"
                     /locus_tag="OO590_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6637333.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            13843..14112
                     /gene="tatA"
                     /locus_tag="OO590_001957"
     CDS             13843..14112
                     /gene="tatA"
                     /locus_tag="OO590_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6637334.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTISDKQADTNQEQAKTEDAKRHDKEQV"
     gene            14116..14631
                     /gene="tatB"
                     /locus_tag="OO590_001958"
     CDS             14116..14631
                     /gene="tatB"
                     /locus_tag="OO590_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6637335.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14634..15410
                     /gene="tatC"
                     /locus_tag="OO590_001959"
     CDS             14634..15410
                     /gene="tatC"
                     /locus_tag="OO590_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6637336.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15452..16234
                     /gene="tatD"
                     /locus_tag="OO590_001960"
     CDS             15452..16234
                     /gene="tatD"
                     /locus_tag="OO590_001960"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6637337.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16231..16719)
                     /gene="rfaH"
                     /locus_tag="OO590_001961"
     CDS             complement(16231..16719)
                     /gene="rfaH"
                     /locus_tag="OO590_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6637338.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            16886..18379
                     /gene="ubiD"
                     /locus_tag="OO590_001962"
     CDS             16886..18379
                     /gene="ubiD"
                     /locus_tag="OO590_001962"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6637339.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18425..19126
                     /gene="fre"
                     /locus_tag="OO590_001963"
     CDS             18425..19126
                     /gene="fre"
                     /locus_tag="OO590_001963"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6637340.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19219..20382)
                     /gene="fadA"
                     /locus_tag="OO590_001964"
     CDS             complement(19219..20382)
                     /gene="fadA"
                     /locus_tag="OO590_001964"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6637341.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20392..22581)
                     /gene="fadB"
                     /locus_tag="OO590_001965"
     CDS             complement(20392..22581)
                     /gene="fadB"
                     /locus_tag="OO590_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6637342.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22771..24102
                     /gene="pepQ"
                     /locus_tag="OO590_001966"
     CDS             22771..24102
                     /gene="pepQ"
                     /locus_tag="OO590_001966"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6637343.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24102..24716
                     /locus_tag="OO590_001967"
     CDS             24102..24716
                     /locus_tag="OO590_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6637344.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24755..26206
                     /gene="trkH"
                     /locus_tag="OO590_001968"
     CDS             24755..26206
                     /gene="trkH"
                     /locus_tag="OO590_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6637345.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26218..26763
                     /gene="hemG"
                     /locus_tag="OO590_001969"
     CDS             26218..26763
                     /gene="hemG"
                     /locus_tag="OO590_001969"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6637346.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6194 a         6868 c         7417 g         6487 t
ORIGIN      
        1 cctgaaaggc ttactgacca atctcgccaa tccgaaagcg attatctatt ttggttcggt
       61 attctcattg tttgtcggcg ataacgttgg cactaccgag cgctggggca ttttcgcgtt
      121 gattattgtc gaaacactgg cgtggtttac cgtcgttgcc agtctgtttg ccctgccgca
      181 aatgcgccgt ggttatcaac gtctggcgaa gtggattgat ggttttgccg gggcgttatt
      241 tgccggattt ggcattcatt tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa
      301 gtcggataag gcgtttacgc cgcatccgac attatttttc acgcatgcct cgccgatgct
      361 aacagcgctc ccaccagcat aaacaacgag ccgaaaatct tattcagcgc cttcatctgc
      421 tttggtcctt taatccatag agcaatcctt tgagcaaggg tggcgtaacc gatcatcaca
      481 ataatatcga ccacaatagt ggtgacgccg agcacgatat actgcatcag ttgcggctgt
      541 tgcggcatga tgaattgcgg aaatagcgcc gccagaaaca caatactttt gggattggtg
      601 agattcacaa aaactgcgcg ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat
      661 ttaagatcaa ttgcgccagc ggcgcgccac tgctggattc ccagccaaat caagtaagcc
      721 gcgcctgccc actttaacac ttcaaacgca atcactgagc gggaaaatag cgtccccaac
      781 cccacgccaa ccagcacaat atgaatcgcc agtccggtct gaagcccagc aatagacgcc
      841 accgcgccgc gataaccgtg gttgagcgag gtggtcatag tgttgattgc accagagcct
      901 ggcgacaggc ttaaaatgat cgatgtcagc aggtaggcaa accaccattc taaggtcatg
      961 atgaactccc ggtgtgtcta tttttgtgcc acaatacgct actgtcgcag cgttgtgtca
     1021 ggcacgctta aaaaaacgat tttacgtggt ttaagaggca gattacccga tgtttcagca
     1081 gcaaaaagac tgggaaacaa gagaaaacgc gtttgctgct tttaccatgg gaccgctgac
     1141 tgatttctgg cgtcagcgtg atgaagcaga gtttactggt gtggatgaca ttccggtgcg
     1201 ctttgtccgt tttcgcgcac agcaccatga ccgggtggta gtcatctgcc cggggcgtat
     1261 tgagagctac gtaaaatatg cggaactggc ctacgacctg ttccatttgg ggtttgatgt
     1321 cttaatcatc gaccatcgcg ggcagggacg ttccggtcgc ctgttagccg atccgcatct
     1381 cgggcatgtt aatcgcttta atgattatgt tgatgatctg gcggcattct ggcagcagga
     1441 ggttcagccc ggtccgtggc gtaaacgcta tatactggca cattcgatgg gcggtgcgat
     1501 ctccacatta tttctgcaac gccatccagg tgtatgtgac gccattgcgc taactgcgcc
     1561 aatgtttggg atcgtgattc gtatgccgtc atttatggca cggcagatcc tcaactgggc
     1621 cgaagcgcat ccacgtttcc gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt
     1681 gccgtttgct atcaacgtac tgacccacag cagacagcga tatcgacgta acttacgctt
     1741 ctatgctgat gacccaacga ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag
     1801 tattctggct ggcgaacagg tgttagccgg tgcgggtgat gacgccacgc caacgcttct
     1861 cttacaagct gaagaggaac gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact
     1921 ccgcaccgcc gcgggccatc ctgtcgaagg aggacggccg ttggtaataa aaggtgctta
     1981 ccatgagatc ctttttgaaa aggacgcaat gcgctcagtc gcgctccacg ccatcgttga
     2041 ttttttcaac aggcataact cacccagcgg aaaccgctct acagaggttt aaatttctta
     2101 tgtaccaggt tgttgcgtct gatttagatg gcacgttact ttctcccgac catacgttat
     2161 ccccttacgc caaagaaact ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg
     2221 cgaccggtcg tcaccacgtt gatgtggggc aaattcgcga taatctggag attaagtctt
     2281 acatgattac ctccaatggt gcgcgcgttc acgatctgga tggcaatctg atttttgctc
     2341 ataacctgga tcgcgacatt gccagcgatc tgtttggcgt agtcaacgac aatccggaca
     2401 tcattactaa cgtttatcgc gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga
     2461 tgcgcttttt taaagaagcg gtgttcaaat atgcgctgta tgagccggga ttactggagc
     2521 cggaaggcgt cagcaaagtg tttttcacct gcgattccca tgaacaactg ctgccgctgg
     2581 agcaggcgat taacgctcgc tggggcgatc gcgtcaacgt cagtttctct accttaacct
     2641 gtctggaagt gatggcgggc ggcgtgtcga aaggccatgc gctggaagca gtggcgaaga
     2701 aactgggcta cagcctgaag gattgtattg cgtttggtga cgggatgaac gacgccgaaa
     2761 tgctgtcgat ggcggggaaa ggctgcatta tgggcagtgc gcaccagcgt ctgaaagatc
     2821 tgcatccgga gctggaagtt attggtacta atgccgacga cgcggtgccg cattatctgc
     2881 gtaaactcta tttatcgtaa tcgttcttta tttggtcagt tgtcaacctg atacttcgct
     2941 acaatggata ctcgttaatc aaagagtttt ccattgtggc gctacttatc atcaccacga
     3001 ttctgtgggc cttctccttt agcttttatg gcgagtacct tgcggggcac gtcgatagct
     3061 attttgcggt gctggtgcgc gttggcttgg cggcactcgt ttttctgccg tttctgcgta
     3121 cccgtagcaa tagcctgaaa acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg
     3181 gcgtgatgta tatgctgagt ttccgcgctt atctctacct gacggtctcc gaattgttgc
     3241 tgttcaccgt actgacgccg ctctacatca cgctgattta tgacatcatg agtaagcgcc
     3301 gtctgcgctg gggctatgct tttagcgcct tgctggcggt gattggtgcc gggattattc
     3361 gctatgatca ggtcaccgac catttctgga ctggcttgtt gctggtgcaa ctctccaata
     3421 tcacttttgc catcggcatg gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc
     3481 agcataacgc ctttgcgtgg ttctatcttg gcgcgttcct ggtggcggta attgcatggt
     3541 tcttgctggg aaatgcgcag aaaatgccgc aaaccacgtt gcaatggggc attctggtgt
     3601 ttcttggcgt ggtggcttcc gggattggct actttatgtg gaactacggc gcgacgcagg
     3661 tggacgccgg aacgctgggc attatgaata atatgcacgt tccggcaggg ctgctggtaa
     3721 acctggctat ctggcaccaa cagccgcact ggccaacgtt tattacaggc gcgctggtga
     3781 tcctggcctc actgtgggtg catcgtaagt gggtcgctcc gcgctcttca caaacggcag
     3841 atgatcgcag gcgtgattgc gcgctgagcg aataaacgct tccgtaactg gctgacgctg
     3901 ctcgccatcg cgcacggcgg cgtacagtcg gctccacaag ccttcgccca gggttttggt
     3961 caccaccaga ccctggcgct caaaactctc tactacccaa tgcggtagcg cggcaatacc
     4021 catccgcgcg gcaaccattt gaatcaataa taaggtgtta tcgacgcttt tcagtgacgg
     4081 gctgacgcct gccggctgaa gaaaatgccg ccagacatcc agtcgactac gctgcaccgg
     4141 ataaattaat agcgtctcgc tggcgagatc ttccggtgta atacgcgttt ttgccgccag
     4201 tggatggtca ggtgctaaca ccaggcgtac ttcataatcg aacatcggcg aataatgcag
     4261 gccactgcgc ggcaggatat cggaggtcat caccagatcc agctcgccct gttgcaacgc
     4321 gggctgcggg tcaaacgtca cgcccgattt aaaatccatc tctacctgcg gccagttctt
     4381 atggaaattt tctaaagcgg gtgtcagcca ctgaatacag ctatggcact caatggcaat
     4441 gcgcagacgc gtctgctgcg gttcattgca ggcttgcagt gcctggctga tttgcggcag
     4501 cacctggttt gccagttgca acaggatttc tccctgcggt gtaaagcgta gcggctggct
     4561 cttacgcaca aatagccgga agccaaggcg ttgttccaga tcgctaaact ggtgagacag
     4621 ggcggattgc gtctgatgca acgttgccgc agcggctgcg agcgagccgc agttccgcaa
     4681 cgcttgtagc gttttcaggt gttttacttc gatcatgaaa gtccttcact tcggcatgaa
     4741 taatttgcgc ttgaggaata tacagtaacc gccaattatg gatgtgtaaa catctggacg
     4801 gctaaaatcc ttcgtctttt aaatttatgg tgcgttggct gcgtttctcc accccggtca
     4861 cttacttcag taagctcccg gcgatgaata aacttgccgc cttccctaaa ttcaaaatcc
     4921 ataggattta catataatta gaggaagaaa aatgacaata ttgaatcaca ccctcggttt
     4981 ccctcgcgtt ggcctgcgtc gcgagctgaa aaaagtgcaa gaaagttatt gggcggggaa
     5041 ctccacgcgt gaagaactgc tgacagtagg gcgtgaactg cgtgctcgtc actgggatca
     5101 acaaaagcaa gcgggtatcg acctgctgcc ggtgggcgat tttgcctggt acgatcatgt
     5161 actgaccacc agtttgctgc tgggtaatgt tccgccacgt catcagaaca aagacggttc
     5221 ggtagatatc gacaccctgt tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc
     5281 ggcggcagcg gaaatgacca aatggtttaa caccaactat cactacatgg tgccggagtt
     5341 cgttaaaggc caacagttca aactgacctg gacgcagctg ctggaggaag tggacgaggc
     5401 gctggcgctg agccacaacg ttaagcctgt gctgctgggg ccggttactt acctgtggct
     5461 ggggaaagtg aaaggtgaac agtttgaccg ccttagcctg ctgaacgaca ttctgccggt
     5521 ttatcagcaa gtgctggcag aactggcgaa acgcggcatc gagtgggtac agattgatga
     5581 acccgcgctg gtactggaac taccacaggc gtggctggac gcatacaaac ccgcttacga
     5641 cgcgctccag ggacaggtga aactgctgct gaccacctat tttgaaggcg taacgccaaa
     5701 tctcgacacg attactgcgc tgcctgttca ggggctgcat gtggaccttg tacatggtaa
     5761 agatgacgtt gttgaactgc acaagcgcct gccttctgac tggctgctgt ctgcgggtct
     5821 aatcaatggt cgtaacgtct ggcgcgccga tcttactgag aaatatgcgc aaattaagga
     5881 cattgtcggc aaacgtgatt tgtgggtggc atcttcctgc tcgttgctgc acagccccat
     5941 cgacctgagc gtggaaacgc gtcttgatgc agaagtgaaa agctggtttg ccttcgccct
     6001 acaaaaatgc catgaactgg cactgctgcg cgatgcgctg aacagtggtg acacggcagc
     6061 tctggcagag tggagcgccc caattcaggc gcgtcgtcac tctactcgtg tacataatcc
     6121 ggcggtagaa aagcgtctgg cggcgatcac cgcccaggac agccagcgtg cgaatgtcta
     6181 tgaagtgcgt gctgaagccc agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac
     6241 gattggttcc ttcccgcaaa ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg
     6301 caatctcgac gccaacaact accgcacggg cattgcggaa catatcaagc aggccattgt
     6361 tgagcaggaa cgtttgggac tggatgtgct ggtacatggc gaggccgagc gtaatgacat
     6421 ggtggaatac tttggcgagc acctcgacgg atttgtcttt acgcaaaacg gttgggtaca
     6481 gagctacggt tcccgctgcg tgaagccacc gattgtcatt ggtgacatta gccgcccggc
     6541 accgattacc gtggagtggg cgaagtatgc gcaatcgctg accgacaaac cggtgaaagg
     6601 gatgctgacg gggccggtga ccatactctg ctggtcgttc ccgcgtgaag atgtcagccg
     6661 tgaaaccatc gccaaacaga ttgcgctggc gctgcgtgat gaagtggccg atctggaagc
     6721 cgctggaatt ggcatcatcc agattgacga accggcgctg cgcgaaggtt taccgctgcg
     6781 tcgtagcgac tgggatgcgt atctccagtg gggcgtagag gccttccgta tcaacgccgc
     6841 cgtggcgaaa gatgacacac aaatccacac tcacatgtgt tattgcgagt tcaacgacat
     6901 catggattcg attgcggcgc tggacgcaga cgtcatcacc atcgaaacct cgcgttccga
     6961 catggagttg ctggagtcgt ttgaagagtt tgattatcca aatgaaatcg gtcctggcgt
     7021 ctatgacatt cactcgccaa acgtaccgag cgtggaatgg attgaagcct tgctgaagaa
     7081 agcggcaaaa cgcattccgg cagagcgcct gtgggtcaac ccggactgtg gcctgaaaac
     7141 gcgcggctgg ccagaaaccc gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt
     7201 gcgtcggggg taaatccaaa ccgggtggta ataccacccg gtcttttctc attacagcga
     7261 cttcttccca ccatactgct taaaccattc cagcatacgc tgccagccat ctttcgcaga
     7321 ttcggcatga tagctcgggc gataatcagc gttgaatgca tgcccggcat ccgggtacac
     7381 gataatctct gctttcgcat tagcagcccg cagcgcctgg cgcatcgttt caacgctctc
     7441 ctgcggaatg ctgttatcct gaccaccata taagccgaga accggtgcgt taagatcggt
     7501 tgcgatatca acaggttgtt tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta
     7561 ccacgccact gcggctttta gctgtggatt atgcgcggca tacagccagg tgatacgtcc
     7621 accccagcag aatccggtga tcattaaacg atgaacatcg ccgccgttgc gggaagccca
     7681 actggcgaca tgatcgagat cggccagcac ctgcgagtca ggcacttttg ctaccagacc
     7741 gctaagcaac gtggggatat cggcaaaatc attcggatcg ccttcgcgga agtaaagttc
     7801 aggtgcgata gccagatacc cctccagcgc cagacggcga caaatgtcgc ggatatgttc
     7861 atgcacgcca aaaatttcct gcactacaat gaccactggc agtgggccat cgctttgctt
     7921 tggtctggca tggtaagcag gcatgttatc cccttgtgaa gggatagagg tgaagcccgc
     7981 cacaatcgcg tcgtccgggg tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc
     8041 aaatccagat tgttgtgttg ttgccatggt attctccgta cccttataaa aatgttgcgc
     8101 aatgttaact atagtcagca tgcaacaaat cacattgcct gaatcggctc atcttttatg
     8161 cagtcctgca gaatgaagag tgatttatgt gatatgaatc acttttggtg ggtaaattta
     8221 tgcaacgcat ttgcgtcatg gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa
     8281 gtgtcttttt gcttcttctg actaaaccga ttcacagagg agttgtatat gtccaagtct
     8341 gatgtttttc atctcggcct cactaaaaac gatttacaag gggctacgct tgccatcgtc
     8401 cctggcgacc cggatcgtgt ggaaaagatc gccgcgctga tggataagcc ggttaagctg
     8461 gcatctcacc gcgaattcac tacctggcgt gcagagctgg atggtaaacc tgttatcgtc
     8521 tgctctaccg gtatcggcgg cccgtctacc tctattgctg ttgaagagct ggcacagctg
     8581 ggcattcgca ccttcctgcg tatcggtaca acgggcgcta ttcagccgca tattaatgtg
     8641 ggtgatgtcc tggttaccac ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca
     8701 ccgctggaat tcccggctgt cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg
     8761 aaatctattg gcgcgacaac tcacgttggc gtgacagttt cttctgatac cttctaccca
     8821 ggtcaggaac gttacgatac ttactctggt cgcgtagttc gtcactttaa aggttctatg
     8881 gaagagtggc aggcgatggg cgtaatgaac tatgaaatgg aatctgcaac tctgctgacc
     8941 atgtgtgcaa gtcagggcct gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc
     9001 cagcaagaga tcccgaatgc tgagacgatg aaacaaactg aaagccatgc ggtgaaaatc
     9061 gtggtggaag cggcgcgtcg tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt
     9121 tcggcctttt gtatttttgc gtagcgcctc gcaggaaatg cctttccaac tggacgtttg
     9181 tacagcacaa ttctattttg tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct
     9241 caatcatggt ttacgcagtt attgcgttgg tgggtgtggc aattggctgg ctgtttgcca
     9301 gttatcaaca tgcgcagcaa aaagccgagc aattagctga acgtgaagag atggtcgcgg
     9361 agttaagcgc ggcaaaacaa caaattaccc aaagcgagca ctggcgtgca gagtgtgaat
     9421 tgctcaataa cgaagtgcgc agcctgcaaa gtattaacac ctctctggag gccgatctgc
     9481 gtgaagtaac cacgcggatg gaagccgcac agcaacatgc tgacgataaa attcgccaga
     9541 tgattaacag cgagcagcgc ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg
     9601 agcacagcaa tcgccgggtt gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc
     9661 cgctacgtga acaactggac ggtttctgcc gtcaggttca ggacagcttc ggtaaagaag
     9721 cacaagaacg ccataccctg acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa
     9781 tggcccagga agcgatcaac ctgacgcgcg cgctgaaagg cgacaataaa acccagggca
     9841 actggggcga ggtagtattg acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg
     9901 aatatgaaac ccaggtcagc atcgaaaatg acgcccgctc gcggatgcag ccggatgtca
     9961 tcgtgcggct gccgcaggga aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct
    10021 atgaacgcta ttttaacgcc gaagacgact acacccgcga aagcgcgcta caggaacata
    10081 tcgcgtcggt gcgtaaccat atccgtttgc tgggacgcaa agattatcaa cagctgccgg
    10141 ggctgcgaac tctggattac gtgctgatgt ttattcccgt tgaacccgct tttttactgg
    10201 cgcttgaccg ccagccggag ctgatcaccg aagcgttgaa aaacaatatc atgctggtta
    10261 gcccgactac gctgctggtg gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc
    10321 aaagccgtaa cgcccagcaa atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt
    10381 tgttcatcga tgacatgtcc gcgattggtc aaagtctcga caaagcgcag gataactacc
    10441 gccaggcaat gaaaaaactc tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt
    10501 ttcgcggttt aggagtagaa attaaacgcg agattaatcc ggatttggct gaacaggcgg
    10561 tgagccagga tgaagagtat cgacttcggt cggttccggg gcagccgaat gatgaagctt
    10621 atcaacgcga tgatgaatat aatcagcagt cgtgctagcc cattaggggt agttaagccg
    10681 ggtagaaatc tagggcatcg acgcccaatc tgttacactt ctggaacaat tttttgatga
    10741 gcaggcattg agatggtgga taagtcacaa gaaacgacgc actttggttt tcagaccgtc
    10801 gcgaaggaac aaaaagcgga tatggtcgcc cacgttttcc attccgtggc atcaaaatac
    10861 gatgtcatga atgatttgat gtcatttggt attcatcgtt tgtggaagcg attcacgatt
    10921 gattgcagcg gcgtacgccg tgggcagacc gtgctggatc tggctggtgg caccggcgac
    10981 ctgacagcga aattctcccg cctggtcgga gaaactggca aagtggtcct tgctgatatc
    11041 aatgaatcca tgctcaaaat gggacgcgag aagctgcgta atatcggtgt gattggcaac
    11101 gttgagtatg ttcaggcgaa cgctgaggcg ctgccgttcc cggataacac ctttgattgc
    11161 atcaccattt cgtttggtct gcgtaacgtc accgacaaag ataaagcact gcgttcaatg
    11221 tatcgcgtgc tgaaacccgg cggccgcctg ctggtgcttg agttctcgaa gccaattatc
    11281 gagccgctga gcaaagccta tgatgcatac tccttccatg tgctgccgcg tattggctca
    11341 ctggtcgcga acgacgccga cagctaccgt tatctggcag aatccatccg tatgcatccc
    11401 gatcaggata ccctgaaagc catgatgcag gatgccggat tcgaaagtgt cgactactac
    11461 aatctgacgg caggggttgt ggcgctgcat cgtggttata agttctgaca ggagaccgga
    11521 aatgcctttt aaacctttag tgacggcagg aattgaaagt ctgctcaaca ccttcctgta
    11581 tcgctcaccc gcgctgaaaa cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga
    11641 ggtaaaaggc ttttcgacgt cattgattct ggtgttcagc gaacgccagg ttgatgtact
    11701 gggcgaatgg gcaggcgatg ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa
    11761 acttcgcgat cgccagcagc ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg
    11821 cgatattcag gtggtgcaaa acttcgttgc gctggcagat ctggcagagt tcgaccctgc
    11881 ggaactgctg gccccttata ccggtgatat cgccgctgaa ggaatcagaa aagccatgcg
    11941 cggaggcgca aagttcctgc atcacggcat taagcgccag caacgttatg tggcggaagc
    12001 cattactgaa gagtggcgta tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga
    12061 gacggctgcc gtcgagcgtg ctgttgatgc cctgaccaaa cggctggaaa aactggaggc
    12121 taaatgacgc ctggtgaagt acggcgccta tatttcatca ttcgcacttt tttaagctac
    12181 ggacttgatg aactgatccc caaaatgcgt atcaccctgc cgctacggct atggcgatac
    12241 tcattattct ggatgccaaa tcggcataaa gacaaacttt taggtgagcg actacgactg
    12301 gccctgcaag aactggggcc ggtttggatc aagttcgggc aaatgttatc aacccgccgc
    12361 gatctttttc caccgcatat tgccgatcag ctggcgttat tgcaggacaa agttgctccg
    12421 tttgatggca agctggcgaa gcagcagatt gaagctgcaa tgggcggctt gccggtagaa
    12481 gcgtggtttg acgattttga aatcaagccg ctggcttctg cttctatcgc ccaggttcat
    12541 accgcgcgat tgaaatcgaa tggtaaagag gtggtgatta aagtcatccg cccggatatt
    12601 ttgccggtca ttaaagcgga tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt
    12661 ttgctgccgg atggtcgccg tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc
    12721 ttgattgatg aactgaattt gctgcgggaa tctgccaacg ccattcagct tcggcgcaat
    12781 tttgaagaca gcccgatgct ctacatcccg gaagtttacc ctgactattg tagtgaaggg
    12841 atgatggtga tggagcgcat ttacggcatt ccggtgtctg atgttgcggc gctggagaaa
    12901 aacggcacta acatgaaatt gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc
    12961 tttcgcgaca gctttttcca tgccgatatg caccctggca acatcttcgt aagctatgaa
    13021 cacccggaaa acccgaaata tatcggcatt gattgcggga ttgttggctc gctaaacaaa
    13081 gaagataaac gctatctggc agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa
    13141 gtggcagagc tacacgtcga ttctggctgg gtgccaccag ataccaacgt tgaagagttc
    13201 gaatttgcca ttcgtacggt ctgtgaacct atctttgaga aaccgctggc cgaaatttcg
    13261 tttggacatg tactgttaaa tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag
    13321 ccgcaactgg tgttactcca gaaaaccctg ctctatgtcg aaggggtagg acgccagctt
    13381 tatccgcagc tcgatttatg gaaaacagcg aagcctttcc tggagtcgtg gattaaagat
    13441 caggtcggta ttcctgccct ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa
    13501 aaaatgccag aactgcctga actggtttac gacagtttgc gccagggcaa gtatttacag
    13561 cacagtgttg ataagattgc ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg
    13621 cgttattttc tcggaattgg cgctacgtta gtattaagtg gcacattctt gttggtcagc
    13681 cgacctgagt gggggctgat gcccggctgg ttaatggcag gtggtctgat cgcctggttt
    13741 gtcggttggc gcaaaacacg ctgatttttt catcgctcaa ggcgggccgt gtaacgtata
    13801 atgcggcttt gtttaatcat catctaccac agaggaacat gtatgggtgg tatcagtatt
    13861 tggcagttat tgattattgc cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc
    13921 tccatcggtt ccgatcttgg tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat
    13981 gaaccaaagc aggataaaac cagtcaggat gctgatttta ctgcgaaaac tatctccgat
    14041 aagcaggcgg atacgaatca ggaacaggct aaaacagaag acgcgaagcg ccacgataaa
    14101 gagcaggtgt aatccgtgtt tgatatcggt tttagcgaac tgctattggt gttcatcatc
    14161 ggcctcgtcg ttctggggcc gcaacgactg cctgtggcgg taaaaacggt agcgggctgg
    14221 attcgcgcgt tgcgttcact ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa
    14281 ctccaggagt ttcaggacag tctgaaaaag gttgaaaagg cgagcctcac taacctgacg
    14341 cccgaactga aagcgtcgat ggatgaacta cgccaggccg cggagtcgat gaagcgttcc
    14401 tacgttgcaa acgatcctga aaaggcgagc gatgaagcgc acaccatcca taacccggtg
    14461 gtgaaagata atgaagctgc gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc
    14521 agttcgccgg aacagaagcc agaaaccacg ccagagccgg tggtaaaacc tgctgcggac
    14581 gctgaaccga aaaccgctgc accttcccct tcgtcgagtg ataaaccgta aacatgtctg
    14641 tagaagatac tcaaccgctt atcacgcatc tgattgagct gcgtaagcgt ctgctgaact
    14701 gcattatcgc ggtgatcgtg atattcctgt gtctggtcta tttcgccaat gacatctatc
    14761 acctggtatc cgcgccattg atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg
    14821 acgtggcctc gccgttcttt acgccgatca agctgacctt tatggtgtcg ctgattctgt
    14881 cagcgccggt gattctctat caggtgtggg catttatcgc cccagcgctg tataagcatg
    14941 aacgtcgcct ggtggtgccg ctgctggttt ccagctctct gctgttttat atcggcatgg
    15001 cattcgccta ctttgtggtc tttccgctgg catttggctt ccttgccaat accgcgccgg
    15061 aaggggtgca ggtatccacc gacatcgcca gctatttaag cttcgttatg gcgctgttta
    15121 tggcgtttgg tgtctccttt gaagtgccgg tggcaattgt gctgctgtgc tggatgggga
    15181 ttacctcgcc agaagactta cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg
    15241 tcgggatgtt gctgacgccg ccggatgtct tctcgcaaac gctattggcg atcccgatgt
    15301 actgtctgtt tgaaatcggt gtcttcttct cacgctttta cgttggtaaa gggcgaaatc
    15361 gggaagagga aaacgacgct gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc
    15421 ccgtcagggc ggttgtcata tggagtacag gatgtttgat atcggcgtta atttgaccag
    15481 ttcgcaattt gcgaaagacc gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa
    15541 tgggctactc atcaccggca ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg
    15601 tcagtattcg tcctgttggt caacggcggg cgttcatcct cacgacagca gccagtggca
    15661 agctgcgact gaagaagcga ttattgagct ggccgcgcag ccagaagtgg tggcgattgg
    15721 tgaatgtggt ctcgacttta accgcaactt ttcgacgccg gaagagcagg aacgcgcttt
    15781 tgttgctcag ctacgcattg ccgcagaatt aaacatgccg gtatttatgc actgtcgcga
    15841 tgcccacgag cggtttatga cattgctgga gccgtggctg gataaactgc ctggtgcggt
    15901 tcttcattgc tttaccggca cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta
    15961 tatcggcatt accggttggg tttgcgatga acgacgcggg ctggagctgc gggaattgtt
    16021 gccgttgatt ccggcggaga aattgctgat cgaaacagat gcgccgtatc tgctcccgcg
    16081 cgatctcacg ccaaagccat catcccggcg caacgagcca gcccatctgc cccatatttt
    16141 gcaacgtatt gcgcactggc gtggagaaga tgccgcatgg ctggctgcca ccacggatgc
    16201 caatgtcaaa acactgtttg ggattgcgtt ttagagtttg cggaactcgg tgttcttcac
    16261 actgtgctta atctctttat taataagatt aagcaatagc atggagcgag cctcaccatc
    16321 gggttcggtg aaaatggcct gaaagccttc gaacgcgcct tcggtaataa tcaccttatc
    16381 acccggataa gggggggccg gatcgacaat gtctttcggt ttatataccg atagctgatg
    16441 aatcaccgcc gatgggacta tcgctggcga cgcgccaaag cgcacaaagt ggctgacgcc
    16501 acgcgtagcg ctgatggtcg tggtatgaat cacttctgga tcaaattcca caaacaggta
    16561 gttagggaat aacggttcac tgactgcggt acgtttaccc cgtacgattt tttccagggt
    16621 gatcatcgga gcgaggcaat taaccgcctg tctctcaagg tgttcctggg cacgttgaag
    16681 ttgcccgcgc ttgcagtaca gtaaatacca ggattgcata atgactctta tccgtttaat
    16741 cggggcgcaa ggatagcaaa agctttacgc taagttaatt atattccccg gtttgcgtta
    16801 taccgtcaga gttcacgcta atttaacaaa tttacagcat tgcaaagatg aacgccgtat
    16861 aatgggcgca gattaagagg ctacaatgga cgccatgaaa tataacgatt tacgcgactt
    16921 cctgacgctg cttgaacagc agggtgagct aaaacgtatc acgctcccgg tggatccgca
    16981 tctggaaatc actgaaattg ctgaccgcac tctgcgtgct ggtgggcctg cgctgttgtt
    17041 cgaaaaccct aaaggctact caatgccggt gctgtgcaac ctgttcggta cgccaaagcg
    17101 cgtggcgatg ggcatggggc aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt
    17161 ggcgtttctg aaagagccgg agccgccaaa aggtttccgc gacctgtttg ataaactgcc
    17221 gcagtttaag caggtgttaa acatgccgac aaagcgactg cgtggtgcgc cctgccaaca
    17281 aaaaatcgtc tctggcgatg acgtcgatct caatcgcatt cccattatga cctgctggcc
    17341 ggaagatgcc gcgccgctga ttacctgggg gctgaccgta acgcgcggcc cgcataaaga
    17401 gcggcagaat ctgggcattt atcgccagca gctgattggt aaaaacaaac tgattatgcg
    17461 ctggctgtcg catcgcggcg gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg
    17521 cgaacgtttc ccggtttctg tggcgctggg tgccgatcct gccacgattc tcggtgcagt
    17581 cacccccgtt ccggatacgc tttcagagta tgcgtttgcc ggattgctac gcggcaccaa
    17641 aaccgaagta gtaaagtgta tttccaatga cctcgaagtg ccagccagtg ccgaaatcgt
    17701 cctggaaggg tatatcgatc ctggtgagat ggcgccggaa gggccgtatg gtgaccacac
    17761 aggttactat aatgaagtcg atagtttccc ggtgtttacc gtgacgcata ttacccagcg
    17821 tgaagatgcg atttaccatt ccacctatac cgggcgtccg ccagatgagc ccgcggtact
    17881 gggcgtggcg ttgaacgaag tgtttgtacc gattctgcaa aaacagttcc cggaaattgt
    17941 cgatttttac ctgccgccgg aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa
    18001 acagtacgcc ggacacgcga agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt
    18061 tatgtacact aaatttgtga tcgtttgcga tgatgacgtc aacgcacgcg actggaacga
    18121 tgtgatttgg gcgattacca cccgtatgga cccggcgcgg gatactgttc tggtagaaaa
    18181 tacgcctatt gattatctgg attttgcctc gcctgtctct gggctgggtt caaaaatggg
    18241 gctggatgcc acgaataaat ggccggggga aacccagcgt gaatggggac gtcccatcaa
    18301 aaaagatcca gatgttgtca cgtatattga cgccatctgg gatgaactgg ctatttttaa
    18361 caacggtaaa agcgcctgat gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa
    18421 gcgcatgaca accttaagct gtaaagtgac ctcggtagaa gctatcacgg ataccgtata
    18481 tcgtgtccgc atcgtgccag acgcggcctt ttcttttcgt gctggtcagt atttgatggt
    18541 agtgatggat gagcgcgaca aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg
    18601 gtttatcgag ctgcatattg gcgcttctga aatcaacctt tacgcgaaag cagtcatgga
    18661 ccgcatcctc aaagatcatc aaatcgtggt cgacattccc cacggagaag cgtggctgcg
    18721 cgatgatgaa gagcgtccga tgattttgat tgcgggcggc accgggttct cttatgcccg
    18781 ctcgattttg ctgacagcgt tggcgcgtaa cccaaaccgt gatatcacca tttactgggg
    18841 cgggcgtgaa gagcagcatc tgtatgatct ctgcgagctt gaggcgcttt cgttaaagca
    18901 ccctggtctg caagtggtgc cggtggttga acaaccggaa gcaggctggc gtgggcgtac
    18961 tggtaccgtg ttaacggcgg tattgcagga tcatggtacg ctggcagagc atgatatcta
    19021 tattgccgga cgttttgaga tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa
    19081 tgcgcgggaa gatcgtctgt ttggcgatgc gtttgcattt atctgagata taaaaaaacc
    19141 cgcccctgac aggcgggaag aacggcaact aaaactttat tcagtggcat ttagatctat
    19201 gtcgcatccg gcaagtggtt aaacccgctc aaacaccgtc gcaataccct gacccagacc
    19261 gatacacatc gtcgccagac caaactgaac gtctttgcgt tccatcagat tcagcagcgt
    19321 ggtgctgata cgcgcaccgg aacaacccag cggatgaccc agcgcgatcg caccaccatt
    19381 gaggttgatc ttctcgtcaa tctgctccat tagcccgaga tctttaatgc acggcaggat
    19441 ctgcgcggca aaggcttcgt tcatctcaaa cacgccgata tcgctggcag aaagccccgc
    19501 ttttttcagc gccagcttcg aggccggaac cgggccgtaa cccataatcg atgggtcaca
    19561 accaacgacc gccatcgaac gcacacgagc gcgcggctta agacctaatt catgggcgcg
    19621 gctttcactc atcaccagca tggcagctgc gccatcggaa agtgcagaag atgtgcccgc
    19681 cgttaccgta ccgtttactg gatcaaacgc cggacgcagc gtggcgaggg cttccacggt
    19741 ggtttccggg cgaatcactt cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg
    19801 accaccagtt gggatgattt catttttaaa tgcggccgac tgcgtggcgg cccaggcgcg
    19861 ggcgtgtgac cgcgcggcaa aggcatcctg catttcacgg ctgataccgt gtatacgcgc
    19921 cagcatttct gccgttaagc ccatcatgcc cgccgctttg gctacattgc ggctcaggcc
    19981 ggggtgaaaa tcgacgccgt gactcatcgg cacatggccc atatgctcca cgccgccaac
    20041 cagacatgcc tgcgcatcgc cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat
    20101 ggatgaacca cacaagcgat tgacggtaac cgccgggaca gagtgcggca cttctgccag
    20161 cagcgccgcg ttacgggcga tgttaaaacc ctgctccagc gtctgttgca cacaacccca
    20221 gtaaatatcg tcgagggccg ccgcttccag cgccgggtta cgcgccagca ggctacgcat
    20281 taaatgagcg gagagatctt ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc
    20341 catcggggtg cgaattgcat cgacaatgac aacctgttcc attgtgattc cttaagccgt
    20401 tttcaggtcg ccaaccggac gggctggctc aaccggagga tagtacggtt cgttatgacg
    20461 cgctttatta cgcagacctt ccggcacttc atacagcggg ccgaggtgct gatattgctg
    20521 tgccatatcg aggtattttg cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg
    20581 gaacggaggg aagcccaggc cgtagaccag cgccatatcc gcttccgccg gagtggcgat
    20641 aatgccctct tccagacagc gcaccacttc gttgaccatc gggatcatca tgcgggcgat
    20701 aatctcttct tcgctgaaat cgcgcttcgg ttggctcact tctgccagca ggtcttcaac
    20761 ggcggcgtct tcttctttct tcggcttacc tttgctgtct tctttataac gccagaaacc
    20821 gaggccgttc ttctgaccaa agcggttggc atcaaacagc gcgtcgatgg catcgcggta
    20881 atctttctgc atccgctgcg ggaagcctgc tgccatgaca gcctgagcgt gatgcgcggt
    20941 atcaatgccc acaacgtcca gcagatatgc cgggcccatc ggccagccaa actgtttttc
    21001 catcactttg tcgatcttgc ggaaatccgc gccgtcgcgc agcagctggc tgaaaccggc
    21061 gaaatacggg aacagcacgc ggttaacaaa gaagccgggg cagtcgttaa ccacaatcgg
    21121 cgtcttgccc atcttgctcg cccaggcgac aactttcgcg atggtttcgt cggagctttt
    21181 ctcgccgcga ataatttcta ccaacggcat tcggtggacc gggttaaaga agtgcatccc
    21241 gcagaagttt tccgggcgtt ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt
    21301 gttagacgcc agcacggtat cctggcgtac tttttgttcg gtttctgcca gtacggcttt
    21361 tttcactttc gggttttcaa caaccgcttc taccacaata tccacgcggt caaatccggc
    21421 gtagtcgagc gttgggtgga tcgtggagat cacgccagcc agtttcagac catcgatctt
    21481 gccgcgctca agctgcttgt tcagcagttt cgcggcttcg gtcatgccga gggttaacga
    21541 cttgtcgttg atatctttca tgacaaccgg cacgcctttc cacgcggact ggtaagcgat
    21601 accgccaccc atgatgcctg cgcccagcac agcagcctgt ttcggggttt caacgtcttt
    21661 ggtgagtttc ttcgctttgc cttttacata ttgatcgtta aggaaaatgc cgaccagtgc
    21721 gcgagcttcg ttggtatgcg ccagcgggac aaaacttttg ttttccagat ttaaggcttc
    21781 ttcacgacca aaacgggccg cagcttcaat ggtttttact gcggtgatgg gggccggata
    21841 atgtttcccc gccgtttgcg cgaccattcc tttagcgatg gtgaagctca tggtggcttc
    21901 aatcttgctc agttttagcg gttccagctt cggctgacgt ttcgctttcc agtcgaggtc
    21961 gccgttaatg gcctggcgta aaatcgccat cgccccttca atcagttttt ctgctttgac
    22021 tacgccatcc accagaccga ttttcagcgc ctgatccgcg ccgacatctt taccggcggc
    22081 agtgatttcc agtgcactgt cagcgcccag catacgtggc atacgtacag aaccgccaaa
    22141 gcccggcatg atgcccagtt tggtttccgg cagaccgatg cgcagatccg gcgtcgccag
    22201 acgataatcg gtcgccagca cgcattcgca gccaccaccc agcgcatagc cgttgacggc
    22261 agcaatggtc ggcaccggca gatcttccag gcgattaaac acgctattgg caaagtgcag
    22321 ccactgactt aactgttctt caggaacgag gaacagggac aaaaattcgg tgatatcagc
    22381 accgacgata aaggctgctt tgttcgaacg cagcagcagc ccttttagat ctgattgctg
    22441 ttccagcacg ccgatggcct cgccgaggct ggcgacggtc gcagtgtcga gtttattaac
    22501 tgaacctggg gcatcaaata ccagttcggc aatgccatct tccagccagt caaggtacag
    22561 ggtgtcgcct ttgtaaagca tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca
    22621 gatgcatcgg agtgtgtgtt ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc
    22681 gatctgatca cgctcggcag aaatcacgct ctggatgaac gatgtgctaa gatgcggaga
    22741 cttaaggtca aaaaaacaga agggtaaaaa atggaatcac tggcctcgct ctataaaaat
    22801 catatagcta ccttacaaga acggactcgc gatgcgctgg cgcgcttcaa gctggatgca
    22861 ttacttattc actccggcga gctgttcaac gtttttctcg acgatcatcc ctatccgttt
    22921 aaagtgaacc cgcaattcaa agcgtgggtg ccggtaactc aggtgccaaa ctgctggttg
    22981 ctggtggatg gcgtgaacaa gccgaaactg tggttctatc tgccggttga ttactggcac
    23041 aacgtcgaac cgctgccgac ctccttctgg actgaagatg tggaagtgat cacgctgccg
    23101 aaagccgatg gcattggtag cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt
    23161 ccggtgccgg aacgtgcgct gcaactgggt attgaggcca gcaacatcaa cccgaaaggg
    23221 gtgatcgact acctgcatta ttaccgctcc ttcaaaaccg agtacgaact ggcctgtatg
    23281 cgtgaagcgc agaaaatggc ggtcaatggt catcgtgcgg cagaagaagc gttccgttct
    23341 ggcatgagcg agttcgatat caatattgcc tatctgaccg cgaccggtca tcgtgatacc
    23401 gacgtacctt acagcaacat tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc
    23461 aaactggacc accaggcacc ggaagagatg cgcagcttcc tgctggatgc cggggccgaa
    23521 tataacggct atgcggctga cctgacccgt acctggtcgg caaaaagcga caacgactac
    23581 gcacagctgg tgaaagacgt aaatgatgaa caactggggc tgatcgccac catgaaagca
    23641 ggcgtcagct atgtggatta ccacatccag ttccatcagc gcatcgccaa actgctgcgt
    23701 aaacatcaaa tcatcaccga tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga
    23761 ccgtttatgc cgcacggtat tggccatccg ctgggcctgc aggtgcatga cgtcgccggt
    23821 tttatgcagg atgatagcgg tacacacctc gcggcaccgg caaaatatcc gtacctgcgc
    23881 tgcacccgta ttctccagcc gggcatggtg ttaaccatcg aaccgggtat ctacttcatt
    23941 gaatcgcttc tggcaccgtg gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa
    24001 attgaagcac tgaaaccgtt cggcggcatt cgtatcgaag acaacgtggt gatccacgaa
    24061 aacaacgtgg aaaacatgac ccgggatctg aaactggcgt gatggaaagc tggttaattc
    24121 ctgcggcacc ggtcacggtc gttgaagaga tcaaaaagag ccgcttcata acgatgttgg
    24181 cgcataccga tggcgttgag gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc
    24241 ccgatgcccg ccaccattgc gtggcgtggg tggcgggtgc gccggatgat tctcaacagc
    24301 tgggtttctc tgacgacggg gagccggcgg gaacggcagg taaaccgatg ctcgcccagc
    24361 taatgggcag cggcgtcggg gaaattaccg ctgtggtagt gcgctactac ggcggcatat
    24421 tgcttggcac cggtgggtta gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc
    24481 agctaacgac ccaacgcaag acgccattaa ccgaatatac tttgcaatgt gaatatagtc
    24541 agttaaccgg cattgaagcg ttgctggggc agtgtgacgg caaaattatc aacagtgatt
    24601 atcaggcatt cgttctgctg cgggtggcgc ttccggcggc gaaagtggct gaattttccg
    24661 caaagctggc ggattttagc cgtggttcat tgcaattgtt agcgattgaa gaataatccc
    24721 cacttcgttt tgcagaacta aggaagcggc agagatgcat tttcgcgcca ttacccgaat
    24781 cgttggacta ctggtcatct tattttcagg gaccatgatt atccctgggc tggtagcact
    24841 catctaccgg gatggagcgg gccgcgcttt tacccagacc ttttttgtcg ccctcgccat
    24901 tggctctatg ctgtggtggc cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg
    24961 gtttctgata gtggtgctgt tttggaccgt gctgggtagt gtcggtgcgc tcccttttat
    25021 cttctcggaa agcccgaacc tcacgattac cgatgcgttt tttgaatctt tctctggcct
    25081 gaccaccacc ggggccacta cgctggtggg gctggattcg ctccctcatg ccatcctctt
    25141 ttatcgccag atgctgcaat ggtttggcgg gatggggatc atcgtgttag cggttgcgat
    25201 actgcctatc ctcggcgtgg gtgggatgca gctctatcgc gcagaaatgc ccggcccgct
    25261 gaaagataac aaaatgcgcc cgcgaattgc ggaaacggcg aaaactctgt ggttgattta
    25321 tgtcttgctg accgtcgcct gtgcgctggc gttgtggttt gccggaatgg atgcctttga
    25381 tgccatcggc catagctttg cgactatcgc tattggcggc ttctcgacac atgatgccag
    25441 tatcggttat ttcgacagtc cggctattaa cactatcatt gctatcttcc tgctgatctc
    25501 cggctgtaac tacggtctgc acttttcact gttaagtggg cgtagtctga aggtttattg
    25561 gcgcgatccg gaatttcgca tgtttatcgg cgtacagttt acgctggtgg ttatttgtac
    25621 actcgtactg tggtttcata atgtctacag ttcggcgctg atgacaatta accaggcgtt
    25681 tttccaggtg gtatcgatgg cgacaaccgc cgggtttacg actgacagca ttgcccgctg
    25741 gccgctcttt ttgccggtac tgcttttatg ttcagctttt atcggcggtt gcgccgggtc
    25801 aacgggcggt ggcctgaaag tgatccgcat cctgctgctg tttaagcagg ggaaccgtga
    25861 gctgaaacga ctggtgcatc cgaacgccgt ctatagcatt aagctgggga atcgcgcact
    25921 gccggaacgt atcctcgaag ccgtttgggg atttttctcc gcctatgcat tggtgtttat
    25981 tgtcagtatg ctggcgatta tcgccacggg cgtggatgat ttttctgcct ttgcctcagt
    26041 tgttgcgaca ttgaataacc tggggccagg gcttggcgtg gttgctgata actttaccag
    26101 tatgaacccg gtggctaaat ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt
    26161 ctttacattg ctggtgctct ttaccccgac tttctggcgt gaatgatgga gtaatacgtg
    26221 aaaacattaa ttcttttctc aacaagggac ggacaaacgc gcgagattgc ctcctacctg
    26281 gcttcggaac tgaaagaact ggggatccag acggatgtcg ccaatgtgca ccgcattgaa
    26341 gaaccacaat gggaaaacta tgaccgtgtg gtcattggtg cttctattcg ctatggtcac
    26401 taccactctg cgttccagga atttgtcaaa aaacatgcga cgcgactgaa ttcgatgccg
    26461 agcgcctttt attccgtaaa cctggtggcg cgcaaaccgg agaagcgtac tccacagacc
    26521 aacagctacg cgcggaagtt tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc
    26581 attgccgggg cgctgcgtta cccacgttat cgctggtacg accgttttat gctcaagctg
    26641 attatgaaga tgtcaggcgg tgaaacggat acgcgcaaag aagttgtcta taccgattgg
    26701 gagcaggtgg cgaatttcgc ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa
    26761 taagcataaa gaataaaaaa tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg
    26821 ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa cacgccgccg
    26881 ggtcagcggg gttctcctga gaattccgac agagaaagca aaaataaatg cttgactctg
    26941 tagcgggaaa gcgtattatg cacacc
//
LOCUS       DAJTYF010000043        26126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTYF010000043 DAJTYF010000000
VERSION     DAJTYF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.043"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            164..1147
                     /locus_tag="OO590_001970"
     CDS             164..1147
                     /locus_tag="OO590_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6637347.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1170..2681
                     /locus_tag="OO590_001971"
     CDS             1170..2681
                     /locus_tag="OO590_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6637348.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2706..3704
                     /locus_tag="OO590_001972"
     CDS             2706..3704
                     /locus_tag="OO590_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6637349.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3737..4831
                     /locus_tag="OO590_001973"
     CDS             3737..4831
                     /locus_tag="OO590_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6637350.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGYRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4843..5715
                     /locus_tag="OO590_001974"
     CDS             4843..5715
                     /locus_tag="OO590_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6637351.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5763..6185)
                     /locus_tag="OO590_001975"
     CDS             complement(5763..6185)
                     /locus_tag="OO590_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6637352.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6282..7484)
                     /locus_tag="OO590_001976"
                     /pseudo
     CDS             complement(6282..7484)
                     /locus_tag="OO590_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7494..8306)
                     /gene="hcaB"
                     /locus_tag="OO590_001977"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(7494..8306)
                     /gene="hcaB"
                     /locus_tag="OO590_001977"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6637353.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8303..8623)
                     /locus_tag="OO590_001978"
     CDS             complement(8303..8623)
                     /locus_tag="OO590_001978"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6637354.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8623..9141)
                     /locus_tag="OO590_001979"
     CDS             complement(8623..9141)
                     /locus_tag="OO590_001979"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6637355.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9138..10499)
                     /locus_tag="OO590_001980"
     CDS             complement(9138..10499)
                     /locus_tag="OO590_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6637356.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10635..11525
                     /gene="hcaR"
                     /locus_tag="OO590_001981"
     CDS             10635..11525
                     /gene="hcaR"
                     /locus_tag="OO590_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6637357.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11685..12824
                     /locus_tag="OO590_001982"
     CDS             11685..12824
                     /locus_tag="OO590_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6637358.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12816..14096)
                     /gene="csiE"
                     /locus_tag="OO590_001983"
     CDS             complement(12816..14096)
                     /gene="csiE"
                     /locus_tag="OO590_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6637359.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRITRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTPQQEQIRKMLESA"
     gene            complement(14287..15141)
                     /locus_tag="OO590_001984"
     CDS             complement(14287..15141)
                     /locus_tag="OO590_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6637360.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMLPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15286..16089)
                     /gene="suhB"
                     /locus_tag="OO590_001985"
     CDS             complement(15286..16089)
                     /gene="suhB"
                     /locus_tag="OO590_001985"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6637361.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGDL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16208..16948
                     /gene="trmJ"
                     /locus_tag="OO590_001986"
     CDS             16208..16948
                     /gene="trmJ"
                     /locus_tag="OO590_001986"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6637362.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17218..17706
                     /gene="iscR"
                     /locus_tag="OO590_001987"
     CDS             17218..17706
                     /gene="iscR"
                     /locus_tag="OO590_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6637363.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17818..19032
                     /gene="iscS"
                     /locus_tag="OO590_001988"
     CDS             17818..19032
                     /gene="iscS"
                     /locus_tag="OO590_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6637364.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            19060..19446
                     /gene="iscU"
                     /locus_tag="OO590_001989"
     CDS             19060..19446
                     /gene="iscU"
                     /locus_tag="OO590_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6637365.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19463..19786
                     /gene="iscA"
                     /locus_tag="OO590_001990"
     CDS             19463..19786
                     /gene="iscA"
                     /locus_tag="OO590_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6637366.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19882..20397
                     /gene="hscB"
                     /locus_tag="OO590_001991"
     CDS             19882..20397
                     /gene="hscB"
                     /locus_tag="OO590_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6637367.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20414..22264
                     /gene="hscA"
                     /locus_tag="OO590_001992"
     CDS             20414..22264
                     /gene="hscA"
                     /locus_tag="OO590_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6637368.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22266..22601
                     /gene="fdx"
                     /locus_tag="OO590_001993"
     CDS             22266..22601
                     /gene="fdx"
                     /locus_tag="OO590_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6637369.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22613..22813
                     /gene="iscX"
                     /locus_tag="OO590_001994"
     CDS             22613..22813
                     /gene="iscX"
                     /locus_tag="OO590_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6637370.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22872..24155
                     /gene="pepB"
                     /locus_tag="OO590_001995"
     CDS             22872..24155
                     /gene="pepB"
                     /locus_tag="OO590_001995"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6637371.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKRVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24297..25073
                     /gene="sseB"
                     /locus_tag="OO590_001996"
     CDS             24297..25073
                     /gene="sseB"
                     /locus_tag="OO590_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6637372.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDCALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25568..26050)
                     /gene="sseA"
                     /locus_tag="OO590_001997"
                     /pseudo
     CDS             complement(25568..26050)
                     /gene="sseA"
                     /locus_tag="OO590_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT         6182 a         6544 c         7239 g         6161 t
ORIGIN      
        1 caagcacctt aaaatcacca tcatacacta aatcagtaag ttggcagcat taccctacgt
       61 tcactaaacc ctattaactg taatatttga gcggcacgcg ctgccgctca tcacaaaata
      121 attagaataa tccgttgtta ataaaaaccc tacaggaggc actatgccta aaaaaatgag
      181 aacaacccgt aatctattat taatggctac gctattaggt agcgcactat ttgccagggc
      241 tgcggataaa gaaatgacca ttggtgcaat ataccttgat acccagggat attacgctgg
      301 agtgcgtcag ggcgttcagg atgcggcaaa agattcttca gtacaggtac agttaattga
      361 aactaacgcc cagggggata tttcgaaaga aagtaccttt gttgataccc tcgtggcgcg
      421 taatgtcgat gccattattt tatcggcagt gtctgaaaat ggcagtagcc gtaccgttcg
      481 tcgcgccagt gaagcgggta ttccggtgat ttgctacaac acctgtatta atcaaaaggg
      541 tgtcgataaa tatgtctcgg cgtatctggt cggcgatcca ctggaatttg gtaaaaaact
      601 gggtaacgct gccgccgatt attttattgc caataaaatt gaccagccga aaattgccgt
      661 catcaattgc gaagcctttg aagtttgtgt gcagcgacgt aaaggatttg aagaagtatt
      721 aaaatcccgc gttcccggcg cgcaaattgt cgctaaccaa gaagggactg ttttagataa
      781 agcgatttcc gttggtgaaa aactgattat ctccacgccg gatctcaacg ccattatggg
      841 ggagtcgggc ggtgcgacac tcggcgcggt aaaagcggta cgtaatcaaa atcaggccgg
      901 aaaaattgct gttttcggtt cggatatgac aaccgaaatt gctcaggagc tggaaaacaa
      961 tcaggtgctg aaagcggtag tggatatttc cggtaagaaa atgggcaatg ctgttttcgc
     1021 gcaaacattg aaggttatca ataaacaagc cgacggtgaa aaagtgattc aggtgccaat
     1081 cgatctctat accaaaacgg aagatggtaa acagtggctg gcaacgcacg ttgatggtct
     1141 gccctaatcg ctgattcgca gaggtcccta tgttcacggc aacagaggca gtcccggtag
     1201 caaaagtggt ggcaggaaat aagcgttacc ccggcgtcgt tgcgctggat aacgttaact
     1261 tcacgctcaa taaaggcgaa gttcgtgcgc tgttaggtaa aaacggcgcg ggcaaatcga
     1321 ctctcattcg aatgcttacc ggcagcgaac gcccggatag cggtgatatc tggattggcg
     1381 agacgcgact ggaaggtgac gaagctacgt tgactcgccg tgccgctgaa ctgggggttc
     1441 gtgcggttta tcaggaatta agtctggtgg aagggctgac ggtggcagaa aatctctgcc
     1501 ttggtcaatg gcctcgccgc aacggcatga ttgattacct gcaaatggcg caggatgccc
     1561 aacgttgctt acaggcgctg ggcgttgacg ttagtcctga acaacttgtt tcaacgctaa
     1621 gcccggcgca aaagcaactg gtggaaattg cacgggtgat gaagggcgag ccgcgcgtgg
     1681 tcattcttga tgaacccacc agctcgcttg ccagtgcaga agttgaactg gtgatcagtg
     1741 cggtgaaaaa gatgtcggca ctgggcgtgg cggtgattta tgtcagccac cggatggaag
     1801 aaatccgccg cattgcctcc tgtgccaccg ttatgcgcga tggtcaggtg gcgggcgatg
     1861 tgatgctcga aaacacctcc acgcatcata ttgtgtcgct aatgctaggg cgtgatcacg
     1921 ttgatattgc gccggttgcc cctcaggaaa ttgtggatca ggccgtactg gaagtccgtg
     1981 cgttacgcca taagcccaag ctggaggata tcagctttac gctacgtcgt ggcgaagtgc
     2041 ttggcattgc tggcctgctg ggggcagggc gcagtgaatt gctgaaggcg attgttgggc
     2101 tggagacgta tgaacagggc gaaattgtta tcaacggcga gaaaatcatg cgccccgatt
     2161 acggcgacat gctgaaacgc ggcattggct atacgccaga aaaccgcaaa gaagcgggga
     2221 tcattccctg gctgggcgtt gacgaaaata cagtgctgac caatcggcaa aaaatcagca
     2281 ccaacggtgt gctgcaatgg tccaccatcc gccgcctgac cgaagaggtg atgcagcgga
     2341 tgacggtcaa ggctgccagt agcgaaacac ccatcggcac gctttctggt ggcaatcagc
     2401 aaaaagtggt gatcggtcgt tgggtctatg ccgccagcca gattttgttg ctcgacgagc
     2461 caacgcgcgg cgtcgatatc gaagccaaac agcagattta ccgtattgtc cgtgagctgg
     2521 ctgccgaagg aaaaagcgtg gtgtttatct ccagtgaagt ggaggaattg ccgctggtgt
     2581 gtgaccgcat cctgttatta cagcacggca cgttctcgca ggagtttcac tcgccggtca
     2641 atgtggatga gctgatgtcc gccattctgt ctgtgcactg atgaatttta ccgaggaacc
     2701 tgaagatgtc tgcttcgtca ttaccattgc cgcagggcaa gagcgtctcg ctcaaacaat
     2761 ttgtcagtcg ccatattaat gagatcggtt tgctggtggt gattgccatt ctttatctgg
     2821 tcttctccct gaacgcgcct ggctttatct cattgaataa ccagatgaac gtgctgcgcg
     2881 atgccgccac cattgggatt gccgcctggg cgatgacgct gattattatc tccggtgaaa
     2941 ttgatgtcag cgttgggccg atggtggctt ttgtctcggt gtgcctggca tttttgctgc
     3001 aatttgaagt tccgttggcg attgcttgtc tgctggtgtt gctgttaggc gcgctgatgg
     3061 gaacgctcgc cggggtgctg cgcggcgtgt ttaacgtacc aagtttcgtt gccacactgg
     3121 ggctgtggag cgctctgcgc ggaatggggc tgtttatgac caacgcacag ccagtgccga
     3181 ttgacgaaaa cgaggtgctg gactggctgg gcggacaatt tctcggtgtg ccagtatccg
     3241 cgctgatcat gatggtgttg tttgcgctgt ttgtgttcat tagccgaaaa accgccttcg
     3301 ggcgctcggt ttttgccgtt ggcggtaatg ccacggcggc gcagttgtgc ggtattaacg
     3361 ttcgtcgagt gcgcattctt atctttaccc tttcgggatt attagcggcg gtgaccggca
     3421 ttttgttggc ggcacgcctc ggttccggta acgcaggtgc cgcaaacggt ctggagtttg
     3481 acgtcatcgc cgcggtcgtg gtcggcggta cggcactttc cggcggtcgc ggttccctgt
     3541 tcggtacatt gcttggtgtg ctggtgatta cgctaatcgg taacggtctg gtgctgctcg
     3601 gtattaactc ctttttccag caggtggtac gcggcgtcat catcgtggtg gcggtgctgg
     3661 cgaatatctt gctgacccag cgaagcagta aagcgaaacg ctaaacctta acgcccctgg
     3721 cgcgggtgcg gcagggatga aaattaatcg tattgttaat gagggattta tgaaaacgat
     3781 gctggcagct tatttaccag gaaattcaac cgtcgatctg cgggaagttg cggtgccgac
     3841 gccggggatt aaccaggtac tgatcaaaat gaaatcctcc gggatttgcg gaagcgatgt
     3901 ccactatatc tatcatcaac accgtgccac ggcggcggca cccgataaac cgttatacca
     3961 gggatttatc aacggtcatg aaccgtgcgg gcagattgtg gcgatggggc aaggctaccg
     4021 ccattttaaa gagggcgacc gcgtgctggt gtatcacatt tctggctgtg gtttttgccc
     4081 gaactgccgc cgcggctttc ctatctcttg tactggcaaa ggaaaagcgg cttacggctg
     4141 gcagcgtgac ggcggtcatg ccgaatatct gctggcggaa gaaaaagatc tgatcctcct
     4201 gccggatgcg ctgagctacg aagatggtgc gtttatcagt tgcggcgttg gcacggccta
     4261 tgaagggatt ttgcgcggcg aagtttccgg cagcgataac gtgctggtgg tcggtctggg
     4321 ggcggtcggc atgatggcga tgatgctggc gaaaggtcgc ggtgcaaaac ggatcatcgg
     4381 cgttgatatg ctgccggaac gtctggcgat ggcaaaacag ttagggggga tggatcacgg
     4441 ctatttagca accaccgaag gtctgccgca gataattgct gaacttaccc acggtggcgc
     4501 ggatgttgcg ctcgattgtt ccggtaatgc cgcaggtcgc ttgctggcac tgcaatccac
     4561 tgctgactgg gggcgggtgg tttacattgg tgaaaccgga aaagtggaat tcgaggtcag
     4621 cgctgatctg atgcaccatc aacggcggat tatcggctcc tgggtgacca gtctgttcca
     4681 tatggaaaaa tgcgcccacg atttgactga ctggaagctg tggccgcgta acgccattac
     4741 ccatcgcttc tcgctagaac aggcaggtga tgcctatgtg ctgatggcga gcggcaaatg
     4801 cgggaaagtt gtgattaact tcccggatta aggagttagc ggatgaccat ctatacctta
     4861 tcccacgggt cgctaaagct ggacgtttct gaccagggcg gcgtaatcga aggcttctgg
     4921 cgcgatacga cgccgctctt acgtcctggt aaaaaaagcg gtgtggcaac cgatgcctca
     4981 tgttttccgc tggtgccatt tgcaaaccgg gtgagcggca accgttttgt ctggcagggg
     5041 cgtgagtatc aactgcaacc gaatgttgag tgggatgcgc actatctaca cggcgatggc
     5101 tggctgggcg agtggcaatg tgtctcgcac agcgatgata gtttgtgtct ggtgtatgaa
     5161 catcgcagcg gtgtctatca ctatcgggta agtcaggcgt ttcatttaac ggcggatacg
     5221 ctgacggtga cgctctctgt caccaatcaa ggggcagaga cgctgccatt tggtaccggc
     5281 tggcatcctt attttccgtt gtcaccacaa acgcggattc aggcgcaggc gagcggttac
     5341 tggctggaac gggagcagtg gctggcgggt gagttttgcg agcagctacc gcaggaactg
     5401 gattttaacc agctcgcgcc gttgccgcgt cagtgggtga acaatggttt tgccggatgg
     5461 aatggtcagg cccgcatcga gcagccgcag gaaggttatg ccatcatcat ggaaacgacg
     5521 ccacctgcac cgtgttattt catctttgtt tccgaccctg cgtttgataa aggatatgcg
     5581 tttgatttct tctgtctgga accgatgagc catgcgccgg atgatcatca tcgcccggaa
     5641 gggggtgatc tcattgcgct agcaccaggg gaatcaacaa cctcagagat gtcgttgcgg
     5701 gtagagtggc tgtaatttat aaaatgccgg ataaggcgtt cacgccgcat ccggcatctt
     5761 tcctaacgcc gatcgagaga aattgccccc ggtccggtaa ttgccaatag caagaacgcg
     5821 ccagcgatac tgacattctt ccagaaatta atcatatttg gcccaaccgc atcgccggtc
     5881 atatcccagt aatggtgacc aatcaccgcc gtacccagcg tgtagaaaat aaacagcacc
     5941 gccagcggac gggtgaaaaa gccaagcacg attaatatcg cggcgggcac ttccataact
     6001 accgcaataa tcgctgccag catcggcatt ggcgcgccca acgaggccat atattggacc
     6061 gtaccgtcaa agcccatcat tttgggaaaa ccaaaaataa tgaaaattaa gaccacggcg
     6121 atacgggcaa ttaataacaa aacggggcgg gcagctccaa aatcaaaata acgtagtgtg
     6181 ttcataacca atccctttgc aagataatgt gatttaaacg taatacacac tcttgcacct
     6241 cgccacgccc caggcattga gatattcgct aattatcctg gttacagtga tttaagcgcg
     6301 atgttctcat ctatcagcag tttcgcatca aacgttttgc cgctctggat ccatttgcga
     6361 attgggcgaa tctcacgccc ctgattcagc gtgaccgcac cgataagcac gccgttttgc
     6421 agattaaacc aaatcgcctt ctgagtttct gggttgccac gacaaagcta gtcatcgcca
     6481 cgcatatcgc caataaactg taagttatca ctgtactgat cgctccagaa ccacggcggc
     6541 ggcagtagcg gtagcggtag ccctaacatt gcggcagcgg caatttgcgc ctggttattg
     6601 gcgttttccc agctttcgca gcggtgtagt gcaccattat caagacgagt gattgccaca
     6661 tcgccaccgg caaagatcgc gggatcgcag gtgcggcaag cctcatcaat gacaatgcca
     6721 ttggcagtat caaggttggc ctcgcgagcc agttgctcgt tggcgctgat actaataccg
     6781 taaatcacca catcagcctg aagcgtttcc ccactttgca gcgtcagttc tactttttca
     6841 ccatcgacca catgttcaat ggcattattg agcagaatgc gcacaccagc ctgctgatgg
     6901 cgctgtaaaa gatagcgttg cacgggcggt ggtgcattac ggcccatgac ggttgccgcc
     6961 agttcaatca ctgtcacctt acatctgcgc tgcgtggcgc tggcagccag ttccagacca
     7021 atagttccgg caccgacaat cacgactgac cgttcgggct gcagaacttc tcgcagtctg
     7081 gcggcatcac cggcatggtg tagggtaaag cagcgttctc ccagtgcatc aagcaacggc
     7141 agcggtcgag ctgccgcgcc ggttgctata aaaagctgat cccagtgcca gctttcgccg
     7201 ttggttaaca ctaactctcg tgtgtcgcga cccagcgttt tgatggttac accggaatgc
     7261 agatgaacat tgttttcctg ccaccagtta gcgggtaaca cctgctgtaa ctgcggggaa
     7321 tcttccagca acatggattt cgagagtgga ggtcgttcat aaggaagatg tcgctcatcg
     7381 gaaaacagat gcagctcacc ggtgaaccct tgctggcgta gcgaggccgc agccattgcc
     7441 gccgcttgcc cgccaccgac aataatgatc gttttttctt tcatcgtgct tccttaaaga
     7501 tccagcccag ccgctacgtg gcgaatgccg cgaatcgcta aacccgcatc agcgttgatc
     7561 atcacaccgc ttaatgcgcg attattgcgc cgcgatgcca acatcacata cggccccgta
     7621 aaatccgccg gttgcgggaa aaattgcagc ggtaaaatgg cggcaatttt ctccggcgtc
     7681 agagactgca ttatcgaggt ttcactttgc ccgagcgcct gtgggccgcg caggtcgctg
     7741 gccataccac acgggccgac gccattcacc cgcactttcg gtgccagttc ataagccagt
     7801 tggcgaataa gtccggttgc ggcatgttta ctggtggtgt acagcgggcc accgccgcca
     7861 ggataccagg cggcatttga cagtgtgaaa atcatgctgc cttcactggc gattaacgcc
     7921 ggagcgcagg cttttgcgcc cagcaggtaa ccgagtacgt tgacgttaaa cagctcgtgg
     7981 aagccggttt cgagcgtctc tgcgggagta ttaaccagtg aggcattgtg atcccagatg
     8041 cctgcattgc cgataaaaca atccagcttg ccggaacgag tcaggatctg atcgaccgcg
     8101 cgttgataat cggcataaca ggtcacgtta ccttccaccg ccagaatatg ttcgccaaat
     8161 cgctgacgca gactggcgac ttttgccgcc gacagttcca gcgcggcaac ctgcgcgcct
     8221 tcttcgataa atcgctcgac cagcgccagc cctaatcccg atccgccgcc ggtaataaaa
     8281 atggactcgt tatgcagatc gctcatggct gcgcctccgg taagtcgatg aaaatgtcac
     8341 caccttcaac gtgtactgga taagtggtga gcggatcggt ggcgggcagg cataacgctt
     8401 tccccgtttt caggcaaaaa ctggcggcgt gtagcgggca ctccaccgtg gcgtcatctt
     8461 ccagataccc ttctgacatc gacgcattac catggctgca acgatcgtta atggcataaa
     8521 actcgccgcc cacgttgaac aaggcgatga cgggcgaggt atctatccgg agagcctcac
     8581 cctccggcac atccgcaacg ggacacgcat aaattcgatt catcagaaca gtacactcag
     8641 gttatgggaa gtgatcaccg cttgatccag gacgatatcc cgttccagca agcgccagtt
     8701 gtcatcttcc agacggcgaa ctttgtcgaa acgcgttcca acatagaatg tttcatcgcg
     8761 ctctttttgt gcccgataaa gtagataatt caccctcacg gcaaatacgt ttgggatgtc
     8821 ggtttcgctt acctggcagt tgctgattaa gtgacgagtg cgtgacggcg gctcttctgc
     8881 ccaggccatg cccgtttcca gacgggcgat tcgccgctcc agctggtctt tggtgtcatt
     8941 gaaaatccag gtcgtcggcg gctggacgcc tttgcgacgg tcgcgtgttt gcgcgttaac
     9001 cgtggtgcgc atggtgtaac gaatctcttc gtcgagctgt gccaaccagt cacgaaattt
     9061 ccagtcatcc agcagactgg cttcgtgaaa gagaaactgg ctaatgcggt gatgtaactc
     9121 cagtgaaact tgcgcactca tttcatcacc tcctgctggt aagcggcggt tttatcgagc
     9181 acttcctgcc agctttcgct actcagaaga tcggcccagc gttggtacat tccacgagcg
     9241 gcagtttctg aaaagatata gttagtaatg ccaggaatgc cgtcgtcgcg gcgcttttcc
     9301 tgaccaagcc ccatttccag acacagtttg ctgttgcggg cgcggtggcc tttaagcaat
     9361 ttctggattt cacaccagtt ctccgagtca tcctgctcga gaaaaccagc aggaccaaaa
     9421 gcacgagtgg cgctgttttc aaaagcggct ttaacttcat cggaggcggc tttgtcagta
     9481 atacagaacg cccacacttc aacttgatca gggccgcgcg gatgccagac gcggagtgtg
     9541 gcagtgccgt ttagccatga aagcgtgggg aatatattgt tatgacccgc caggcgcagg
     9601 gcgcgaactt cacctaaacg ttgttctgct tcggcatagg tttcgcggta atagcttgaa
     9661 actgcgccat cgacccagac attagcatcc ggtttttcag taaagaagaa accgctaccg
     9721 tgaccgtcct gaccaaattg cagcgcatct ttggcggttt cccacaccgg gcgggcggtt
     9781 tgtccatcac cgaggcgctt atcgctgcca tcatctttcg cccctaatac ctgaacggca
     9841 gaagcatggc tgaacagagc atgatactgg tcactggcga actgctctgc cgggaatttc
     9901 cagttacagt tgatcaccca cttttgtacg ccgccgacaa tttcggtgcc gccttcgcga
     9961 cgatccagca tgccatccag ataccaggca atgtcaccca ggtaatcatg caggcccggt
    10021 gcgctggtat cccagttgcc aaaaatcagt cctttataac tctccacaca aggaacttcg
    10081 tttagccccc agtgggattt acacaaccct tgtgggtagg cgcgaggttc cagcggtaca
    10141 tcgatcaact cgccgttaat gccataagac cagccgtgat acgggcaggt aaaggcgcga
    10201 gtgttgccac aatctgcata actcacacgc atggcccggt ggcggcattg gttaagaaac
    10261 gccttgatgc tgccgtcttt ctggcgcacc acgacaactg catcttctcc catgtaggtg
    10321 ttaaagaaat caccgggttt tgggatctgg ctttcgtggg cgagaaatag ccagcaacga
    10381 ccgaaaatac gctcaagctc cagttggtaa atgtccgggt cggtataaat acgcggagtg
    10441 acccgaccgt tttgggtgtc aatcagttgg taaatgttca aatctgaggg tgtggtcata
    10501 ttttttaatc ctgaaagata agatgtattg accttctatt tttgtgccat aaggatgttg
    10561 ctggttggtt gctaatatgt gaaatagtta atttctacca gctgagacgt tttttcgata
    10621 acaaggggaa ggtgatggaa ctacggcatt tacgctattt cgtcgcagtg gcgcaggcac
    10681 tgaactttac ccgtgcggcg gaaaaactgc atacctcaca gccttcgtta agcagccaga
    10741 tccgcgatct tgaaaactgt gtcggtgttc cgttactggt gagggataag cgcaaagtcg
    10801 cgttgacggc ggcgggagaa tgttttctcc aggacgcgct ggcaatcctc gaacaagcgg
    10861 aaaatgccaa attacgggcg cggaaaattg ttcaggaaga cagacaatta accattggct
    10921 ttgtgccatc ggcggaagtg aatttattgc caaaagtatt accgatgttt cgtctcagac
    10981 agccagacac cttgattgag ctggtgagtt taatcaccac gcaacaggag gaaaaaattc
    11041 gccgtggtga actcgacgtc ggcttaatgc gccatcctgt ttatagcccg gagattgatt
    11101 atctggagct ttttgacgaa ccattagtgg tggtgttacc ggttgatcac cctttagcac
    11161 atgaaaaaga gatcaccgcc gcgcaactgg atggcgtgaa tttcgtcagt accgatccgg
    11221 cgtattccgg ttcgcttgcg ccgatcgtta aagcgtggtt tgcgcaagaa aacagccagc
    11281 caaatatcgt ccaggtggca acgaatattc tggtgaccat gaatctggtg ggcatggggc
    11341 tgggcgtcac tttgataccc ggttatatga ataattttaa taccgggcag gttgtttttc
    11401 gtcccattgc cggaaatgtg ccttccattg ctttactgat ggcgtggaag aaaggggaga
    11461 tgaagccggc attacgcgat ttcatcgcca ttgtgcagga acgtttggca agcgtaacgg
    11521 cataattacg cattattttt gcaacatgcg ccgcggatcg cagtttttta gtgcttgtga
    11581 actggcgtta cagacatcgg gaagcaggct cgaatattct tatttttcaa cttgcaccaa
    11641 cattcgatta accccagact aacgcctcct gacgggaggg actcatggtt ttgcaatcca
    11701 cgcgctggtt ggcgctcggc tatttcacat acttttttag ttacggcatt tttttacctt
    11761 tctggagcgt ctggcttaaa gggattggtt taacgccaga aaccatcggc ctgttattgg
    11821 gggcaggtct ggttgcccgt tttctcggga gtttgctcat cgcgccccgc gtcagcgatc
    11881 cttcccgcct gatttccgcc ttgcgcgtgc tggcactgct gccacttctc tttgctgtcg
    11941 ccttctgggc gggggcgcac gtagcgtggc tgatgctggt gatgattggc tttaacctct
    12001 ttttctcacc gctggtaccg ttgaccgatg cactggcgaa tacgtggcaa aagcagttcc
    12061 cgcttgatta cggcaaagtg cgactgtggg gctcggtggc gtttgtcatt ggctcggcgc
    12121 tgacgggcaa actggtcact atgtttgatt atcgggtgat cctcgtgctg ttgacgttgg
    12181 gcgtggcatc catgctgctc ggctttctca tccgtccgac gattcagcca caaggggcaa
    12241 gccgccagca ggagagcacc ggttggtctg cgtggttggc gctggttcgc cagaactggc
    12301 gctttctggc ctgcgtttgt ttattgcagg gggcacatgc ggcctattac ggttttagcg
    12361 ccatttactg gcaggcagct ggctactcgg cctcggcggt ggggtatttg tggtcgctgg
    12421 gcgtggtggc ggaagtcatt atctttgcgc tgagtaataa acttttccgc cgttgtagtg
    12481 ctcgcgatat gctgttgatc tcggcgattt gcggcgtagt gcgctggggc attatgggag
    12541 caactacggc gttgccgtgg ttgatagtgg tgcaaattct gcattgcggc accttcacgg
    12601 tctgccacct ggccgccatg cgttatattg ctgctcgcca ggttagcgaa gtcatccgtt
    12661 tacaggcggt ttactctgcc gtcgcgatgg gcggcagtat cgctatcatg accgttttcg
    12721 ccggtttcct gtatcaatat ctgggccacg gtgtgttctg ggtaatggcg ctggtggcgc
    12781 ttccggcaat gtttttgcgc ccgaaagttg ttccctcatg ctgattcgag cattttgcgg
    12841 atctgctcct gttgaggtgt tgtcagcgtc aggtccgtat agatcagcgg tggtgaaaag
    12901 agcggtaacg gcatggtata aggcgcaata atcagtgccg cgccacgcgg agcgcctgtc
    12961 tgcaaaaatg cctttaccga catatgctta atgttgagcg gcagtaacgt tagttcgcgt
    13021 aactgctgct caatctgcgc ttctcgctcg ctatcattcc cggtcagtag aataatctgt
    13081 ttttcatgca gatcgttttc ctgcattagc caggcaccga aaatcaccgc gaccagacca
    13141 ctttcctcat cagaaagatg gacgccgtat tcactttcaa atccggccag cgccgcgcgg
    13201 gtgatgcgta ccaggcgtgg atacagtctg gcgaactctt ccggcagggt attatcaata
    13261 ccgatggcaa acaaactgcg atttaacgcc tgggcgaggt ggatataaag ctgatcacat
    13321 aacccctgtt catcataaaa acgaacattt cccagctcac gaaaatggtt taccagacgt
    13381 ttgatagact gacgcaattg tctgtcccgc tgatgcgcat cccgcagtgg atcgggaacc
    13441 cgcaacatgg aaaaaagtaa tgccataaac agtggctcat caggcggtac atgctggagc
    13501 gcccgacgct gccagtggcg tccaatttcc tgcgctacct gaaattcaag gcaggattcc
    13561 gcccagcgac gttggagcgg attaaactga ggcgtaattc cagcgtggtg ctgcagcaaa
    13621 caatattgca gatacagaca caggaaatga atatcgcgcg attcaaagcg tttttgcagc
    13681 cgtcgggagc agagattcac taacgcttgt agattggtat cgtcgtaaaa gttacgcgca
    13741 attccgcgcc gcttcagttc actcttcagg gcgggggtga attggctggt aataaatgac
    13801 ggacacagac ggaaaccacg tcgtagccag tgaaataaac acaaacgttg gttaagcact
    13861 gtaccttcaa cccggtagct accgtcataa ccagtcgtca gtgtgagttg atgatagcgc
    13921 aggatctccc gtcctgtttc gctgatatca agactggcaa tatcatcatc cacaccatta
    13981 agctcgctga aggttgccat ggtggcggtc aaccccggct gaaagagcgt cagcaagatc
    14041 tggcagcggc gctggggagc cgaaaggaca gatggtggag caagcgtagg catcatctgt
    14101 agatctccca taagcataat tgataagaat agctaaagtt tgctcaggga aatggcgaag
    14161 ggaatgctaa tcatcagaaa tgttggcaag cgtcacagaa tttttctcat tttaaactgt
    14221 tctgaccagt ggattagcat gtcttaaggt ggttttgtga atgattatcg tgctagcgat
    14281 catgggttag ttctgcggat tcaacgcgct aaggataaag tccaccatct gttcgcgata
    14341 aacattgccg tgacgatcgg aaaaagcatc aatgtgttcg ccatccggga ttaggatcaa
    14401 ccgttttggc tcttttgcca ggctatacaa cttttcgctg tgctgccatg ggataacgtg
    14461 atcggcttta ccgtgaatga gtaaaagcgg gatcgggctg acgctggcga tgtaattttc
    14521 gccgctgtaa ctctcatcaa gtaagtagcc actgccgggg agcatctggt tggcgatggt
    14581 tgcataagag gcaaatgtgg agtcgaggat caccgcacgt atgccttcac gatcaccctg
    14641 accaataaca gcaagaatat tcgccccgcc aatactctgc ccgaacagca ccagccgttg
    14701 tgggtttaca tcactgcgat ggcgcaccac attgatggcg ctttgcgtat cgtccagcaa
    14761 tccggcctgg gacggagtgc cttttgattt accaaacccg cgataatcaa acataaaaac
    14821 gttgaaatta cgctcaggta accaactgac cagcggccag tgggcggaca tatttccggc
    14881 attgccgtga gcatgaatga tggttgcgat ggcgttgtca gcagggcccg tcgaagaagg
    14941 aataaaccag ccttgcagac gagtaccgtc cttagccgta aattcgacgg attccgccga
    15001 ccaggggtcg ggaccgtaaa ttttatcgtc aggataatag aagaagttga tggcaacgcg
    15061 cagcactaaa taaacgcaga aggcaacaac aaagagaata aacagaattt tgggaacgcg
    15121 tttgttcact ggcagtgcca taacctttcc ttgcgacttc tttatcatcc attaatcggg
    15181 gaaagatggt gggagagtga cgaaagaaag taaagcggtg aggaagtatt tcggaatagc
    15241 gcctgaaagg cgagggcggg tgagtgatat cacccgcctg agtcattaac gcttcagagc
    15301 gtcgcttaac tcgtcacgca tgttcgccag tatggctttc acaacacgcg ggttaccagc
    15361 aacgatgtta ccggtcagca tatagttatg accaccggtg aagtcgctga cgatgccgcc
    15421 cgcttcacga accagcagat cgcctgccgc gaaatcccac ggacgcagac cgatttcaaa
    15481 gaaaccgtca acacgacccg cagcgacgta agccagatcc agcgccgcag aaccggtacg
    15541 acggaagtct gcacattcgt tgaacagctt gccgacgatg ttgatgtagg tagtggcgta
    15601 ctgttttgct ttgaacggga agccggtcgc cagaatggta ccgtcgagat cgcgagcggt
    15661 gctaccgcgc agacggtagc cgttcagctg tgcgccctga ccgcgagtgg cggtgaacag
    15721 ttcgttacgc ataggatcgt ataccacagc aacttcggtg cggcctttga tacgaacggc
    15781 gatagatacc gcgaagtgcg gcagacgttt gataaagttg gtagtaccat ccagtggatc
    15841 gataacccat tgaacatcct gatcagtacc ttcaagttca ccgctttctt cggtgatgat
    15901 ggtgtgctgt gggtaagatt tacgaatcgt gtcgataatc accgcttcgg cagctttatc
    15961 tacgttggtc acgaaatcgt tactgccttt ctggctcgct tctacagcgt ccggggtttc
    16021 atagtttttg gcaattaaat tacccgcctt gcgcgctgcg cgcacggcga tgttcagcat
    16081 cggatgcatc agtctctctc actggatgtt aaagaacggg aaaacggcgc agagtatagc
    16141 agcgagatcg gcatctgtct tcgttttatg ataagatgcg tgaataatct ttagatgaag
    16201 aaagacaatg ctgcaaaata ttcgaattgt gctggtggag acgtcacaca ccggcaatat
    16261 gggttctgtt gcccgtgcca tgaaaacaat gggattaacc aatctgtggc tggttaatcc
    16321 actggtgaaa cccgactccc aggcgattgc cctggctgca ggggccagcg atgtgattgg
    16381 taatgctcac atcgtcgata cgctcgacga agcgttagcc ggttgtagcc tggtggtggg
    16441 caccagcgca cgctcccgca cgctgccgtg gccgatgctc gacccgcgcg aatgcggtct
    16501 gaaaagcgtc gctgaagcgg caaatacccc ggtggcgctg gtgtttggtc gtgagcgcgt
    16561 cggcttgacc aatgaagagt tgcagaaatg ccattatcat gtcgcgattg cggcaaaccc
    16621 ggaatacagt tcgctgaacc tggcaatggc ggttcaggtt attgcctatg aagtgcgtat
    16681 ggcctggctg gcaactcagg aaaatggcga gcaggtcgag catgaagaga cgccgtatcc
    16741 gctggtcgat gatctggagc gtttttatgg tcatctggaa caaacgctgc tggcaaccgg
    16801 ttttatccgt gaaaaccatc cggggcaggt gatgaataaa ttgcgccgtc tgtttacccg
    16861 tgcgcgcccg gaaagccagg aattgaatat cctgcgcggg attctggctt ctattgagca
    16921 gcagaataaa ggtaacaagg ccgaataaca gtggttgcct gatgcgacgc ttaacgcgtc
    16981 ttatcaggcc tacaatgtac agaatcgtag gtcggataag gtgttcacac cgcatccgac
    17041 aacaggtaca aacgccacga taaaaaaatg gcactgaagg ttaaataccc gactaaatca
    17101 gtcaagtaaa tagttgacca atttactcgg gaatgtcaga cttgaccctg ctatgcaata
    17161 cccccacttt tacaataaaa aaccccgggc aggggcgagt ttgaggtgaa gtaagacatg
    17221 agactgacat ctaaagggcg ctatgccgtg accgcaatgc ttgacgttgc gctcaactct
    17281 gaagcgggcc cggtaccgtt ggctgatatt tccgaacgtc agggaatttc cctttcttat
    17341 ctggaacaac tgttttcccg tctgcgtaaa aatggtctgg tttccagcgt acgtggacca
    17401 ggcggtggtt atctgttagg caaagatgcc agcagcatcg ccgttggcga agtgattagc
    17461 gccgttgacg aatctgtaga tgccacccgt tgtcagggta aaggcggctg ccagggcggc
    17521 gataaatgcc tgacccacgc gctgtggcgt gatttgagcg accgtctcac cggttttctc
    17581 aacaacatta ctttaggcga actggttaat aaccaggaag tgctggatgt gtctggtcgt
    17641 cagcatactc acgacgcgcc acgcacccgc acacaagacg cgatcgacgt taagttacgc
    17701 gcttaataaa aagaattcag aatcaggccg gagtgctaaa tactccgtaa acacggtcgt
    17761 acatccagcc ggtagcctga ttccttgcat tgagtgatgt acggagttta tagagcaatg
    17821 aaattaccga tttatctcga ctactccgca accacgccgg tggacccgcg tgttgccgag
    17881 aaaatgatgc agttcatgac gatggacgga acctttggta acccggcctc tcgttctcac
    17941 cgtttcggct ggcaggctga agaagcggta gatatcgccc gtaatcagat tgccgatctg
    18001 gtcggcgctg atccgcgtga aatcgtcttt acctctggtg caaccgaatc tgacaacctg
    18061 gcgatcaaag gtgcagccaa cttttatcag aaaaaaggca agcacatcat caccagcaaa
    18121 accgaacaca aagcggtact ggatacttgc cgtcagctgg agcgcgaagg tttcgaagtc
    18181 acctacctgg caccgcagcg taacggtatt atcgacctga aagaactcga agcggcgatg
    18241 cgtgacgaca ccatccttgt ttccatcatg cacgtaaata acgaaatcgg cgtggtgcag
    18301 gatatcgcgg ctatcggcga aatgtgccgt gctcgtggca ttatctatca cgttgatgca
    18361 acccagagcg tgggcaaact gccaatcgac ctgagccagt tgaaagttga cctgatgtct
    18421 ttctccggtc acaaaatcta tggcccgaaa ggtatcggtg cgctgtatgt gcgtcgtaaa
    18481 ccgcgtgtac gcatcgaagc gcaaatgcac ggcggcggtc acgagcgcgg tatgcgttcc
    18541 ggcactctgc ctgttcacca gatcgtcgga atgggcgaag cctatcgcat cgcaaaagaa
    18601 gagatggcga ccgagatgga acgtctgcgc ggcctgcgta accgtctgtg gaacggcatc
    18661 aaagatatcg aagaagttta cctgaacggt gacctggaac acggtgcgcc gaacattctc
    18721 aacgtcagct tcaactacgt tgaaggtgag tcgctgatta tggcgctgaa agacctcgca
    18781 gtttcttcag gttccgcctg tacgtcagca agcctcgaac cgtcctacgt gctgcgcgcg
    18841 ctggggctga acgacgagct ggcacatagc tctatccgtt tctctttagg tcgttttact
    18901 actgaagaag agatcgacta caccatcgag ttagttcgta aatccatcgg tcgtctgcgt
    18961 gacctttctc cgctgtggga aatgtacaag cagggcgtgg atctgaacag catcgaatgg
    19021 gctcatcatt aatcggtatc ggaatcagga gaatttataa tggcttacag cgaaaaagtt
    19081 atcgaccatt acgagaatcc gcgtaacgtg ggttcctttg ataacaacga cgagaacgtc
    19141 ggcagcggca tggtgggtgc accggcctgt ggcgacgtaa tgaagttgca gattaaagtc
    19201 aacgatgaag gtatcattga agacgcgcgt tttaaaactt acggctgcgg ttccgctatc
    19261 gcttccagct ccctggtcac cgaatgggtg aaagggaagt ctctcgacga agcgcaggcg
    19321 atcaaaaaca ccgatattgc tgaagaactt gaactgccgc cggtgaaaat tcactgttct
    19381 attctggcag aagacgcgat caaagccgcc attgcggact ataaaagcaa acgtgaagca
    19441 aaataagagt tgaggtttgg ttatgtcgat tacactgagc gacagtgcag cagcgcgagt
    19501 aaataccttt ctggctaacc gcggcaaagg gtttggcctg cgtctgggcg tgagaacctc
    19561 cggatgttca ggtatggctt atgtactgga atttgttgac gaaccgacgc cggaagacat
    19621 cgtgtttgaa gacaaaggcg tgaaagtcgt ggtcgatggc aaaagcctgc aatttctgga
    19681 cggtacgcag ctggacttcg taaaagaagg cctgaatgaa gggtttaaat tcaccaaccc
    19741 gaacgttaaa gatgagtgtg gttgcggcga aagcttccac gtttgatgcg cataccgata
    19801 accccaccgt ggtcgcctgc gcgtggggtt tgttttacct gattcgccgt tatcgcggcg
    19861 gatcgcagcc ctgagaatgt tatggattac ttcaccctct ttggcttgcc tgcccgttat
    19921 caactcgata cccaggcact gagcctgcgt tttcaggatc tacaacgtca gtatcatcct
    19981 gataaattcg ccagcggaag cctggcggaa caactcgccg ccgtacagca atctgcaacc
    20041 attaaccagg cctggcaaac gctgcgtcat ccgttaatgc gcgcggaata tttgctttct
    20101 ttacacggtt ttgatctcgc cagcgagcag catactgtgc gcgacaccgc gttcctgatg
    20161 gaacagttgg agctgcgcga agagctggac gagatcgaac aggcgaaaga tgaagcgcgg
    20221 ctggaaagct ttatcaaacg tgtgaaaaag atgtttgata cccgccatca gttgatggtt
    20281 gaacagttag acaacgaggc gtgggacgcg gcggcggata cggtgcgtaa gctgcgtttt
    20341 ctcgataaac tgcggagcag tgccgaacaa ctcgaagaaa aactgctcga tttttaattt
    20401 ctggaagcta aacatggcct tattacaaat tagtgaacct ggtttgagtg ctgcgccgca
    20461 tcagcgtcgt ctggcggctg gtattgacct gggcacaacc aactcgctgg tggcgacagt
    20521 gcgcagcggt caggccgaaa cgttagccga ccatgaaggc cgtcacctgc tgccatctgt
    20581 tgttcactat caacagcaag ggcattcggt gggttatgac gcgcgtacta acgcagcgct
    20641 tgacaccgcc aacaccatta gttctgttaa acgcctgatg gggcgctcgc tggctgatat
    20701 ccagcaacgc tatccgcatc tgccttatca attccaggcc agcgaaaacg gcctgccgat
    20761 gattgaaacg gcggcggggc tactgaaccc ggtgcgcgtt tctgcggaca tcctcaaagc
    20821 actggcggcg cgggcaactg aagccctggc aggcgagctg gatggtgtag ttatcaccgt
    20881 tccggcgtac tttgacgatg cccagcgtca gggcaccaaa gacgcggcgc gtctggcggg
    20941 gttgcatgtt ctgcgcttac ttaacgaacc gaccgctgcg gctatcgcct acgggctgga
    21001 ttccggtcag gaaggcgtga tcgctgttta tgacctcggt ggcgggacgt ttgatatttc
    21061 cattctgcgc ttaagtcgcg gcgtgtttga agtgctggca accggcggtg attccgcgct
    21121 cggcggcgat gatttcgacc atctgctggc ggattacatt cgcgagcagg cggacattcc
    21181 tgatcgtagc gataaccgcg ttcagcgtga actgctggat gccgccattg cagccaaaat
    21241 cgcgctgagc gatgcggact ccgtgaccgt taacgttgcg ggctggcagg gcgaaatcag
    21301 ccgtgaacaa ttcaatgaac tgatcgcgcc actggtaaaa cgaaccttac tggcttgtcg
    21361 tcgcgcgctg aaagacgcgg gtgtagaagc tgacgaagtg ctggaagtgg tgatggtggg
    21421 cggttctact cgcgtgccgc tggtgcgtga acgggtaggc gaatttttcg gtcgtccacc
    21481 gctgacttcc atcgacccgg ataaagtcgt cgctattggc gcggcgattc aggcggatat
    21541 tctggtgggt aacaagccag acagcgaaat gctgctgctt gatgtgatcc cactgtcgct
    21601 gggcctcgaa acgatgggcg gcctggtgga gaaagtgatt ccgcgtaata ccactattcc
    21661 ggtggcccgc gctcaggatt tcaccacctt taaagatggt cagacggcga tgtctatcca
    21721 tgtaatgcag ggtgagcgcg aactggtgca ggactgccgc tcactggcgc gttttgcgct
    21781 gcgtggtatt ccggcgctac cggctggcgg tgcgcatatt cgcgtgacgt tccaggtcga
    21841 tgccgacggt cttttgagcg tgacggcgat ggagaaatcc accggcgttg aggcgtctat
    21901 tcaggtcaaa ccgtcttacg gtctgaccga tagcgaaatc gcttcgatga tcaaagactc
    21961 aatgagctat gccgagcagg acgtaaaagc ccgtatgctg gcagaacaaa aagtagaagc
    22021 ggcgcgtgtg ctggaaagtc tgcacggcgc gctggctgct gatgccgcgc tgttaagcgc
    22081 cgcagagcgt caggtcattg acaatgctgc cgctcacctg agtgaagtgg cgcagggcga
    22141 tgatgttgac gccatcgaac aagcgattaa aaacgtagac aaacaaaccc aggatttcgc
    22201 cgctcgccgc atggaccagt cggttcgtcg tgcgctgaaa ggccattccg tggacgaggt
    22261 ttaatatgcc aaagattgtt attttgcctc atcaggacct ctgccctgat ggcgctgttc
    22321 tggaagctaa tagtggtgaa accattctcg acgcagcgct gcgtaacggt atcgagattg
    22381 aacacgcctg tgaaaaatcc tgtgcttgca ccacctgcca ctgcatcgtt cgtgaaggtt
    22441 ttgactcact gccggaaagc tcagagcagg aagacgacat gctggacaaa gcctggggac
    22501 tggagccgga aagccgttta agctgccagg cgcgcgtcac cgacgaagat ttagtggtcg
    22561 aaatcccgcg ttacactatc aaccatgcgc gtgagcatta acagaggtta gtatgggact
    22621 taagtggacc gatagccgcg aaattggcga agcactgtac gatgcgtatc ccgatcttga
    22681 tccgaaaacg gttcgattca ccgatatgca tcagtggatt tgcgatctgg aagatttcga
    22741 cgacgacccg caggcatcca acgagaaaat cctcgaagcg attttgttag tctggctgga
    22801 cgaagccgag tgaatgaacg ggctgcctgg gggtagcccg ttttgctgtt aaacgaataa
    22861 ggataactaa aatgacagaa gcgatgaaga ttaccctctc tacccaacct gccgacgcgc
    22921 gctggggaga aaaagcaact tacagcatta ataatgacgg cattaccctg catttgaacg
    22981 gggcagacga tctggggctg atccagcgtg cggcccgcaa gattgacggt ctgggcatca
    23041 agcatgttca gttaagcggt gaaggctggg atgcggatcg ctgctgggca ttctggcaag
    23101 gttataaagg cccgaaaggc acgcgtaaag tggagtggcc ggatctggac gatgcccagc
    23161 gccaggaact ggataaccgc ctgatgatca tcgactgggt gcgtgacacc atcaacgcac
    23221 cggcagaaga attgggacca tcgcaactgg cacagcgtgc tgttgatctg atcagcaacg
    23281 tcgcgggcga tcgtgtgact tatcggatca ccaaaggcga agatctgcgt gagcaaggtt
    23341 atatggggct gcacactgtc gggcgcggtt cagaacgttc tccggtattg ctggcgctgg
    23401 attacaaccc aactggcgat aaagaagcac cggtgtacgc gtgcctggta ggtaaaggta
    23461 tcacttttga ctccggcggc tacagcatca aacagactgc gtttatggac tcgatgaagt
    23521 cggacatggg cggcgcggca acggttaccg gggcgctggc atttgccatt acgcgcggac
    23581 tgaacaagcg cgtgaagctg ttcctctgct gtgcggataa cctgattagc ggcaatgcgt
    23641 tcaagctggg cgatatcatc acttatcgca acggtaaaag agttgaagtg atgaacactg
    23701 atgccgaagg gcgtctggtg cttgccgatg gtctgattga tgccagtgcg cagaaaccgg
    23761 aaatgatcat tgatgcggcg accctcaccg gggcggcgaa aactgcgctg ggtaatgatt
    23821 atcacgcgct gttcagtttt gacgatgcgc ttgccggtcg tttactggcg agtgcctcac
    23881 aagagaacga accattctgg cgtctgccgc tggcggaatt ccaccgcagc cagctgccgt
    23941 ctaactttgc cgaactgaac aataccggaa gcgcggcgta tccggcaggc gcgagcacgg
    24001 cggcgggctt cctgtcgcac tttgttgaga actatcagca aggctggctg catatcgact
    24061 gctcggcgac ttaccgtaaa gcgccggttg aacagtggtc tgcgggcgct acgggacttg
    24121 gtgtgcgcac gatagctaat ctgttaacgg cgtaaactaa tatgccggat gcggcgtaaa
    24181 cgccttatcc ggcctacaaa ttctgcaaat tcaacaaatt gcgaatccct tgtaggcctg
    24241 ataagcgtag cgcatcaggc atttgcgccc cgaacgttga atgaattgtg gttcttatgt
    24301 ccgaaacaaa aaacgaactt gaagacctgc tggaaaaagc agcaaccgaa ccggcgcacc
    24361 gcccggcctt tttccgtact ctactggaat ccaccgtctg ggtgcctggt acggcggcgc
    24421 agggcgaggc tgtggttgaa gattgtgcgc ttgatttaca gcactgggaa aaagaagacg
    24481 gcaccagcgt cattcctttt ttcacctcgt tagaagcgct tcaacaggcg gttgaagacg
    24541 aacaggcatt tgtcgtaatg cccgttcgca cattgtttga gatgaccctt ggcgaaacgc
    24601 tcttccttaa tgccaaactg ccgaccggta aagaatttat gccgcgtgaa atcagtttgt
    24661 tgattggtga agagggaaat ccgctgagca gccaggaaat cctggaaggc ggtgaatcgc
    24721 tgatattatc ggaagtcgca gagccgccag cacaaatgat tgattcactc accaccttat
    24781 ttaaaaccat taagccggtg aagcgtgctt ttatttgttc aattaaagag aacgaagagg
    24841 cacagcctaa tttacttatt ggcattgaag ccgatggtga tatcgaagaa attattcagg
    24901 cgacgggaag tgtagcgacc gatacattac ctggcgatga accaatcgat atttgtcagg
    24961 tgaaaaaagg ggaaaaagga attagccact ttattaccga acatattgcg ccattctatg
    25021 aacgtcgctg gggtggtttt ttgcgtgact ttaaacaaaa ccggataatc taaaatatgc
    25081 cgccctaaag ggcggcattc gggcttactt gctgctctga aggttgttaa ccaccagtaa
    25141 attgtcgccg gagagactcc ggttattatt tacttgggaa aacagcgcac cagaaacaat
    25201 aagtacgata cagaagcacc gtatcttcat tgtgctgtcg tgatgcgaag accgcatcac
    25261 gacagcacca ctggcgatat tgccgcgata gcaagcgctg agacgcgctt gctctaacgg
    25321 tcgcggaaca tcctttcctt aagccagcgc ccgcttgcga gacggcggaa agggccgcag
    25381 tggataatac actaacagaa caataataac tctcttttgc ttgacaaaaa gagagttact
    25441 ggcgagtatt gtctccgctg agacgcgctt gcattactac ggtaatacaa cctgctggaa
    25501 aggccgcgaa ccagaccagc aataaaaaaa cgccaaattc ggcggttttt tattgcctgt
    25561 aaaatactta tttcactggc tcaaccggta aatctgcccg cgcgccccat tcactccatg
    25621 cgccgtcgta cagtttcacg tttggcacat ccagcgtcgc gagtgctaac aaaaccacgg
    25681 ctgccgttac accagagccg cagctgacga taatcggttt gtcatagctg acgccgcgac
    25741 caaaaaatat cgcatccagt tcatcggtcg tttttagttc gccttcgcgc accagttccg
    25801 tccacggaac gttcaatgct ccgggaatat gtccgcgacg taaacctggg cgaggttcat
    25861 caacttctgc gttaaaacgt gcagccgggc gggcatcaat aatttgcgcc gtattttcat
    25921 ggcttgccaa taacacatcg gttactttca ccactgcttc aggattaaac gcggcgttaa
    25981 actctccttc cggcagctct actgcacctt cttctaatag caggtcatcg cgctgccagc
    26041 ctgcaagtcc ccgttacttt attcgtaccc cttataatgg ggtgttagcc agccagaccc
    26101 ggcatgatta ctgcccccag tcgtcc
//
LOCUS       DAJTYF010000044        25890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTYF010000044 DAJTYF010000000
VERSION     DAJTYF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.044"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            348..641
                     /locus_tag="OO590_001998"
     CDS             348..641
                     /locus_tag="OO590_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6637373.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="OO590_001999"
     CDS             713..1372
                     /locus_tag="OO590_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6637374.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="OO590_002000"
     CDS             1464..1910
                     /locus_tag="OO590_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6637375.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO590_002001"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO590_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="OO590_002002"
     CDS             3390..3809
                     /locus_tag="OO590_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6637376.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQRL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5075
                     /gene="umuC"
                     /locus_tag="OO590_002003"
                     /pseudo
     CDS             3809..5075
                     /gene="umuC"
                     /locus_tag="OO590_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(5121..5651)
                     /gene="dsbB"
                     /locus_tag="OO590_002004"
     CDS             complement(5121..5651)
                     /gene="dsbB"
                     /locus_tag="OO590_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6637377.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5797..7338)
                     /gene="nhaB"
                     /locus_tag="OO590_002005"
     CDS             complement(5797..7338)
                     /gene="nhaB"
                     /locus_tag="OO590_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6637378.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7559..8278
                     /gene="fadR"
                     /locus_tag="OO590_002006"
     CDS             7559..8278
                     /gene="fadR"
                     /locus_tag="OO590_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6637379.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8330..9862)
                     /locus_tag="OO590_002007"
     CDS             complement(8330..9862)
                     /locus_tag="OO590_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6637380.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHTCYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10192..11490
                     /gene="dadA"
                     /locus_tag="OO590_002008"
     CDS             10192..11490
                     /gene="dadA"
                     /locus_tag="OO590_002008"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6637381.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11500..12570
                     /gene="dadX"
                     /locus_tag="OO590_002009"
     CDS             11500..12570
                     /gene="dadX"
                     /locus_tag="OO590_002009"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6637382.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12738..14164)
                     /locus_tag="OO590_002010"
                     /pseudo
     CDS             complement(12738..14164)
                     /locus_tag="OO590_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14774..16510)
                     /locus_tag="OO590_002011"
     CDS             complement(14774..16510)
                     /locus_tag="OO590_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6637383.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16605..17519)
                     /gene="ldcA"
                     /locus_tag="OO590_002012"
     CDS             complement(16605..17519)
                     /gene="ldcA"
                     /locus_tag="OO590_002012"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6637384.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17619..18230
                     /gene="emtA"
                     /locus_tag="OO590_002013"
     CDS             17619..18230
                     /gene="emtA"
                     /locus_tag="OO590_002013"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6637385.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18232..18966)
                     /locus_tag="OO590_002014"
     CDS             complement(18232..18966)
                     /locus_tag="OO590_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6637386.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19167..19421
                     /locus_tag="OO590_002015"
     CDS             19167..19421
                     /locus_tag="OO590_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6637387.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19471..20931)
                     /locus_tag="OO590_002016"
     CDS             complement(19471..20931)
                     /locus_tag="OO590_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6637388.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20868..21425)
                     /locus_tag="OO590_002017"
     CDS             complement(20868..21425)
                     /locus_tag="OO590_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR6637389.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARVLTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21451..22305)
                     /locus_tag="OO590_002018"
     CDS             complement(21451..22305)
                     /locus_tag="OO590_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR6637390.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22305..23114)
                     /locus_tag="OO590_002019"
     CDS             complement(22305..23114)
                     /locus_tag="OO590_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR6637391.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23124..23882)
                     /locus_tag="OO590_002020"
     CDS             complement(23124..23882)
                     /locus_tag="OO590_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6637392.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23879..24859)
                     /locus_tag="OO590_002021"
     CDS             complement(23879..24859)
                     /locus_tag="OO590_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6637393.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISP
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24859..25881)
                     /locus_tag="OO590_002022"
     CDS             complement(24859..25881)
                     /locus_tag="OO590_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR6637394.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6450 a         6372 c         6727 g         6341 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgtga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcctgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc ttcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac tactgtatat
     3361 aaaaacagta tagcttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaacggtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt accggtggtt gtgctatcga
     3901 ataatgacgg ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggc gttaaaatgg
     3961 gcgatccctg gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca
     4021 gcaattatga gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat
     4081 cgccccgcgt cgagatttac agtattgatg aagcattctg cgatctgaca ggtgtgcgta
     4141 attgtcgcga tctgactgat tttggcagag aaattcgcgc aacggtgcta caacgtaccc
     4201 atcttactgt tggtgtgggg atcgcccaga ccaaaacgtt ggctaagctt gccaatcatg
     4261 cggcaaaaaa atggcagcgg cagacgggtg gggtggtgga tttatcaaat ctggaacgcc
     4321 agcgtaaatt aatgtctgct ctccccgtgg atgacgtctg ggggattgga cggcggatca
     4381 gcaaaaaact ggacgcgatg gggatcaaaa ccgttctcga tttggcggat acagatatcc
     4441 ggtttatccg taaacatttt aatgtcatgc tcgaaagaac ggtgcgtgaa ctgcgcggcg
     4501 aaccctgttt gcaactggaa gagtttgcac cgacgaagca ggaaattatc tgttcccgct
     4561 cgtttggtga acgcatcacg gattatacgt cgatgcggca ggccatttgt agttacgctg
     4621 cccgggcggc ggaaaaactt cgcagtgagc atcaatattg tcggtttatc tccacattta
     4681 ttaagacgtc accatttgcg ctcaatgaac cttattacgg caatagcacg tcggtaaaac
     4741 tgctgacgcc cactcaggac agcagggata tcattaacgc tgctacgcga tctctggatg
     4801 ccatctggca agcgggccat cgctaccaaa aagcgggcgt gatgctgggg gatttcttca
     4861 gtcagggagt cgcgcagctc aatttattcg atgacaatgc accgcgcccc gggagtgagc
     4921 aattgatggc ggtaatggat actctgaatg ctaaagaggg cagaggaaca ctctattttg
     4981 ccgggcaggg gatccaacag caatggcaga tgaagcgagc tatgctttca ccacgttata
     5041 caacgcgaag ttctgattta ctgcgggtca aataaatata gcggcaggaa aaaagcgttc
     5101 ccgcaggagc gccgaatgga ttagcgaccg aacagatcac gttttttcgc tttaaacggc
     5161 tgggaaatca ccaccagcac cgcgacaatc aggtaagcga taaaaatacc gagcagccac
     5221 tgcggcattt ccatacctaa aaaatcccac tgacgctcgg cgcaatcgcc agaggcgaca
     5281 aacacttgcg gcacccattt atccagcggc agccattccg ggaaacgaac cataaaatca
     5341 caggtggcaa atggcgaagg atagagctga agcatggtgt gctcgtaagt taactgcaca
     5401 ccgcggaacg cactatacaa ccagataacc atcgctacat aacgcagcgg agttttcggg
     5461 gcgatcgcgc caatcagcgc agcacccaga acgccgaata acgcgcagcg ttcataaata
     5521 cagagcacgc aaggtttcag taacatcaca tgctggaacc acagcgccgt cagttccagt
     5581 gccagagcag taaacgccat caacagccac gcgccccggc cttgtgaaca ttggttcaaa
     5641 aatcgcaaca taatcatttc cctgcaatat gcatagagtg cgcagtttaa accaattcat
     5701 tcgctgcgcc accaggaggc agcaaaaata ggacgcaatt ggacaattat cgggcgaaaa
     5761 ggcaaaccgg gcatttatat gcccggtaag ttgttatcaa agcgttgcta tccagcccat
     5821 ttgcataaac cattcggtta caggggcaag cgtaaactcg acgcagagca agccgacgag
     5881 tgtcagtacg agggtgtaag gcagggccat ccacaccatg cggccataag agaggcgaat
     5941 caatggcgcg agtgcagagg tcagcaggaa caggaacgca gcctgaccgt tcggcgtagc
     6001 gacagagggc agattggtac cggtattaat ggcgaccgcc agcagctcgt attgcttcaa
     6061 cgtgatagcg ccactttcca ttgccgcttt cgcttcgttg atataaatcg tccccacgaa
     6121 gacgttatcc gaaatggatg acagcagacc gttgaaaata tagaacagcg acagctgagc
     6181 atgttcagat gcctgcaaca caaactgaat aatcggcgaa aacagttgtt ggtcgataat
     6241 caccgcgact accgagaaaa acaccgtcaa cagtgcggtg aatggcagag attcggtgaa
     6301 ggctttaccg atagcatgtt catcggtgac accggtcaat gatgttgcca gaataatgac
     6361 tgacaaacca atcaagccaa cttccgccag atgcaacgcc agcgcagtca ccagccagac
     6421 gccaataatc gcctggacaa tcagacggat tttatcctga cgggtacgct ggtggcggct
     6481 ttgatcgtca aactgttgca acacttcgcg gactttctcc ggcagcgttt caccgtagcc
     6541 aaaccaacgc agcttctcta ccagcaggca ggttaacagg ccacaaatca gaaccggaac
     6601 ggtcaccggc gacatgcgca ggaagaaatc gccaaaatgc cagccagccg ctttagcgat
     6661 gatcaggttc tgtggttcgc ccaccatggt cattacgccg cctaatgcgg tgccgacacc
     6721 ggcatgcatc atcaggctac gcagaaagcc acggaactgt tccagaacca cgttgtaatg
     6781 cttgtcgata tgactatcgt cttgcaggtc ggtgtcttcg gtacgggaag aggctacgcg
     6841 atgataaata ccataaaaac cgactgcaac gctgatcacc accgccacga cggttaaggc
     6901 atcgaggaac gcggagagga acgctgccgc cacgcaaaaa gagagcgaca gcagcatttt
     6961 ggagcgaatg cttaacagca aacgggtaaa tatgaacagc aacagctgtt tcataaaata
     7021 gatacccgcc accataaaca tcaataacag caagacttca agatttgccg ccaccccttc
     7081 acggacgtgt tccgcgctgg tcatgccgat gaataccgct tcgatagcca acagaccacc
     7141 ggggagtagc gggtagcatt tcagggccat cgccagagtg aaaataaatt ccgctaccag
     7201 caaccagccc gcgacgaaag ggctgatgag gaaaattaac gggtttacga ttaagaaaat
     7261 gatgagggcg agtttgtacc agtcggggga ctggcccaaa aaattgcgcc atagcgcgcg
     7321 gccccaggag atctccatga tggtttccct taccttacaa ataatcaatg atgtttttgt
     7381 gtttaaacgc aaagcttaac ggtcaggcag gagtgaggca agtcttgata gtcaagggga
     7441 aagagatgcg gaaaatgaag ccttgatccc tttttcttct ttttgtctgc tatcagcgta
     7501 gttagccctc tggtatgatg agtccaactt tgttttgctg tgttatggaa atctcactat
     7561 ggtcattaag gcgcaaagcc cggcgggttt cgcggaagag tacattattg aaagtatctg
     7621 gaataaccgc ttccctcccg ggactatttt gcccgcagaa cgtgaacttt cagaattaat
     7681 tggcgtaacg cgtactacgt tacgtgaagt gttacagcgt ctggcacgag atggctggtt
     7741 gaccattcaa catggcaagc cgacgaaggt gaataatttc tgggaaactt ccggtttaaa
     7801 tatccttgaa acactggcgc gactggatca cgaaagtgtg ccgcagctta ttgataattt
     7861 gctgtcggtg cgtaccaata tttccactat ttttattcgc accgcgtttc gtcagcatcc
     7921 cgataaagcg caggaagtgc tggctaccgc taatgaagtg gccgatcacg ccgatgcctt
     7981 tgccgagctg gattacaaca tattccgcgg cctggcgttt gcttccggca acccgattta
     8041 cggtctgatc cttaacggga tgaaagggct gtatacgcgc attggtcgtc actatttcgc
     8101 caatccggaa gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg cgttgtgcag
     8161 tgaaggcgcg cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg agagtggcga
     8221 gatttggcac cggatgcaga aaaatctgcc gggtgattta gccattcagg ggcgataatc
     8281 ccttccgttt aaagagcaaa cccctcaaac gaggggtttt ttgttgtttt tacagatttc
     8341 ccattcttgg cgggcaacgt tccagcaact cgatgctgcc gtcttcgttt tgctgttcga
     8401 gcatcacatc aaatccccac aggcgatgca catgcttcag gacttctttg cgcccccgat
     8461 ccagcggtgc gcgattatgt ggaatataac gcagcgtcag cgaacggtcg ccgcgcaaat
     8521 ccacgttcca gatctgaata ttcggctcca gattacttaa gttatattgc gacgataacc
     8581 ggttacggat ctcccgataa ccttcttcat tatgaatagc ggaaatctcc agataattat
     8641 gccgatcatc atccagcacg gtgaagaagc ggaaatcacg catcactttc ggtgacagaa
     8701 actggctgat aaaactctca tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc
     8761 agtcggaacc ggcgatatcc gggaaccagt atttgtcttc ttccgttggc gactgacaaa
     8821 tccgtttaat atcctggaac atggcgaacc cgagggcata cgggttgatg ccgctgtacc
     8881 acgggctgtt ataggggggc tggaagacca cattggtgtg gctgtgcaaa aactccagca
     8941 taaaacgttc cgttactttc ccttcatcat acagatggtt aaggatggta tagtgccaga
     9001 aggtcgcaca gccttcgttc ataacctgag tctgtttttg cggataaaaa tactggctca
     9061 ccttacgcac aatacgcagg atttcacgct gccatgattc cagcagtggc gcatttttct
     9121 ccataaaata gagcaggttt tcttgtggtt cggacggata gcggcgcgct tcagcaaccg
     9181 ttttctcttc ctcgcgcttc ggcagggtac gccagagcat attgacctga ctttgtagat
     9241 actcttcgcg acttttctgc cgggctttct cttcttgcag cgagattttt tgcgggcgtt
     9301 tgtaccggtc cacgccgtag ttcatcagcg cgtggcacga gtccagaagc cgttctactt
     9361 catcaacgcc ataacgttct tcgcactcgg taatatattt acgggcaaaa atcagataat
     9421 cgacaatcga actggcgtcg gtccaactgc ggaataagca attgttttta aagaaagagt
     9481 tatgcccata gcaggtgtgt gccatcacca gcgcctgcat agtaatggtg ttctcttcca
     9541 tcaagtaagc gatacacggg ttagagttaa tgacgatttc ataggccagc ccttgctgac
     9601 cgtgcttata cagccgttca gtctcgataa actttttacc gaatgaccag tgcggatagt
     9661 taattggcat gccgacgctg gaataggcat ccatcatctg ttctgaggtt atcacttcaa
     9721 tctggtgcgg gtaagtatcc agccggtaga gtttcgccac ccggtctatc tctgccagat
     9781 aaacatccag caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg gtgtccttat
     9841 taatagaatc gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct ctgtgtggag
     9901 cacctcattt caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt
     9961 tgcgatttct tattatcaga gtgccactaa tccgcttctg aacggaattt tatgctggat
    10021 aaaaagggcg ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg
    10081 tgagtcagct caccataaaa aagccgtatg ttgaataata ttttcaactg agttatcaag
    10141 atgtaattag attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt
    10201 gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga
    10261 catgaggtca ccgtcattga tcgggagccg ggggccgccc tggaaaccag tgccgcgaat
    10321 gccgggcaaa tctcccccgg atatgctgca ccgtgggcag caccaggtgt gcctttaaaa
    10381 gcgattaaat ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag
    10441 tttcagttga aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa
    10501 aacaaagggc ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc
    10561 gccgaaacca atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc
    10621 gaacaacagt atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg
    10681 tatcagctgc tggaatccag ccgcctggcg gaagtggatc ccgcgctggc agaagtagcg
    10741 cacaaactga cgggcggcct gcagttaccc aatgatgaaa ccggagactg ccagctgttt
    10801 acccagaatc tggcgcggat ggcagagcag gcgggggtta aattccgctt taatacgccc
    10861 gttgaccaac tgctttgcga cggcgagcaa atctacggcg tgaagtttgg cgatgaagtg
    10921 attaaggccg atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc
    10981 attgttgata ttccggtgta tccgctgaaa ggctactcgc tgactattcc aattgcgcag
    11041 gaagatggtg cgccagtatc caccattctt gatgaaacct acaaaatcgc cattacccgt
    11101 ttcgataacc gcattcgtgt tggcggaatg gcggaaattg ttggttttaa caccgagctg
    11161 ttgcaaccgc gtcgtgaaac gctggaaatg gtggttcgcg atctctatcc acgcggcggt
    11221 catgtcgagc aggcgacttt ctggacgggt ttgcgcccga tgacgccaga cggtacgccg
    11281 gttgtcgggc gtacacgctt taaaaatctg tggctgaata ccggtcacgg cacgctcggc
    11341 tggacgatgg cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca
    11401 gcgatcccat atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt
    11461 ccgggccatt tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc
    11521 agcctcgatc tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac
    11581 gcgcgcgtct ggtcggtggt aaaagcgaac gcttacgggc atggtattga gcgtatctgg
    11641 agcgcgctcg gggccaccga tggctttgca ttacttaacc tggaagaggc aataacgtta
    11701 cgtgagcgcg gctggaaggg gccgatcctg atgctggaag gatttttcca tgctcaggat
    11761 ctggagattt atgaccagca ccgcctgacc acctgcgtac acagcaactg gcagctcaaa
    11821 gcactgcaaa atgcgcggct aaaagcaccg ttggatattt atcttaaagt gaacagtggg
    11881 atgaatcggt tgggcttcca gcccgatcgc gtgcttaccg tctggcagca gttgcgggca
    11941 atggcgaatg ttggcgaaat gaccctgatg tcgcattttg ctgaagcgga acatcctgat
    12001 ggaatttccg gcgcgatggc gcgtattgag caggcggcgg aggggctgga gtgtcggcgt
    12061 tcgttgtcca attcggcggc gactctgtgg cacccggaag cgcattttga ctgggttcgg
    12121 cctggcatta ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat cgccaatacc
    12181 ggattacgtc cggtgatgac gctaagcagt gagattattg gtgtccagac gctaaaagcg
    12241 ggcgagcgtg tgggctacgg cggtcgctat actgcgcgcg atgaacagcg aatcggcatt
    12301 gtcgccgcag gatacgccga cggttatccg cgccatgcgc ctaccggtac tcctgtttta
    12361 gtggacggcg tgcgcaccat gacggtgggg accgtctcga tggatatgct ggcggttgat
    12421 ttaacgcctt gcccgcaggc ggggattggt acgccggttg agctgtgggg caaggagatc
    12481 aaaattgatg atgtcgccgc cgctgccgga acggtgggct atgagttgat gtgcgcgctg
    12541 gcgttacgcg tcccggttgt gacagtgtaa cttgttgtaa gccggatcgg aggcagcgtc
    12601 ttctggttgc gaaaaaatca tccatccggc tggtcagcaa ctgtagttgt taatgtgaca
    12661 gagccattgc ccatgatagt gtccattaaa agaatggaca ctatttcccc ggaacctgaa
    12721 ctcgccgcac aggcgttcta cataaaacgc ttacgcttca ttgttgactc ggagtcgact
    12781 tagataaatc acgcttcacc cttgatgcag attcgggttc atgttcagat tgttgattaa
    12841 caggctgcga gtcctgttgt ctttccagac tcaacaggcc caactgttga tccaggagct
    12901 ccaggtcctc ctgtaccttc acgcgctcca ggagttccgg gtcatccagg aactccagat
    12961 ccataaggtc ctccagctcc tcgagtcctt ccaggtcaac ctagccttcc ttgtgcaaaa
    13021 agtccaaaag gtccttcagg ccctgctgtt ttttcaggcc ctcgacggct ttgagcagcg
    13081 tcggtaaatt gtctttgtcc cacgggttac cattacagtc aaacaccttc aggctctggg
    13141 gctgcggcac ctgtaccgtt ttaaacttgt tttctccaca gtagtaaatt attagtttgg
    13201 gcggcagccg tggtgaagag atcaaatcgt tactagaaca gtcaaatact ttcaggcgag
    13261 tcaagttctc cagcatctca tcaggtattg agttaatatt gttgccggca caggatagtt
    13321 tatacaggta ctggggcatc gacggcggta aaaattgcaa gaagttttca gaacagttaa
    13381 tcactctcag gttagtcgac agctcaggca gccagataat acagttttca tagcacttca
    13441 gtaccatcag ttttgagggc tgcaccttcg gtaacaagat caagacgtta tctgtacagt
    13501 caagcaccag cagccacttt ggcatcacct tcggtaacgc ggtcaacacg tttttactgc
    13561 agtaaaattc aatcagatgt ggaggcaaat tctcagggag tgaagtcaat cccaggttgc
    13621 taagattgag catactccgt cttgtaatga gacattcctt cattctgcgc aatgcttctg
    13681 cccggttttc aatccggctt gctggcaggt tttcttccag gatattttcc ttgatttctt
    13741 ccagactttc tttcaggtct tcttggggaa tttctgactg tttgttttgc tggttttctt
    13801 ccaggatttc gtacaggttt ttttgcaggt tttcttgcaa accatcctgc tgtatagccc
    13861 attcgcacca tttggcgacc atcatgaact cgttatgttg ctgattcagg cgagtcccca
    13921 acgtgatgat ggtattgttg tccacctgta tgttcacttt gcccgcgata ctgaggtcaa
    13981 tgatctgcca gggggcatca ccccagctga atttttgcgg gctgagatac ttcatgttct
    14041 gcaaaatttg attcttgagt tccaccgaca ggaagagccg ccctggcggg cgtcgtacgt
    14101 ctgggttaac ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag
    14161 ccataatgct acgtttcctt gaaataaata tcctctggca gcgccggaga cgtgaatgtg
    14221 gtcaccgcaa aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt
    14281 tttccctggt cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc
    14341 tctgacgtat taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa
    14401 ggaataacta taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg
    14461 tgtgggctat gtcagtgcgg caggttgcgt ggggaatatg ttcaggcgat gtcaggcagg
    14521 ggcccctgat ggttgaccgg taatgcgggg ctggttttta ttacctgtta cagtcggaaa
    14581 aagcccttac gctgactttc ttgagccggt gttttgagtt cattagccag agcctgcaat
    14641 ggtttgtcat ccataattta accttcttct gatgttggga tgagcatatc aaaatcagga
    14701 aatgacaagt ttattacagg ctcaggctgc gttcagtaaa gcatcgcatc cggcgataga
    14761 tgctgcatta aatttaagat tcagcttcct cttcagccac ccgaacgcca atcttcaaca
    14821 cttcattatc ttctttctcg gccaccgtcc agatcatccc ggcaaactct acctggtcac
    14881 cgacaaccgg tgccgcgcct agcaactgct ggacaatttc acccagcgtt tgctgtttat
    14941 cacgatattc tcgcccgtct tccaggccat atatcagcgc aacatcagca tatttggcgc
    15001 tggcttcgag aatgaagtca ccaaagaagc gttgatccag cgcgaccggt ggcgactggc
    15061 tgaacagttt accgagcgcc gggagatcgc gttcccgacc aattacacac aatacatcgc
    15121 cttcgcgcag tcgggtgctg ccggtaggat gaagcaactg gttatcacga aacagggctg
    15181 caatacgcgt ctcttttggc atatgcaaat cgcgcagtgc cgcacctaca caccatttat
    15241 cagcactcaa ttgataaaca aactgctccc acggattttc cggatgaata tccaggccaa
    15301 cgcgtgacac cggacgtccg acgggcggaa ccaccacttt ggcttttttc gccgcccacg
    15361 agagtgatgt tccctgcaag agcagtgaaa ccagaaccac aaagaaggcg acattaaaga
    15421 acagtcgcgc attctccagc cccgccatca tcgggaacac ggccaggatg attggcaccg
    15481 cgccgcgtaa tcccacccag ctgataaaca cgcgctcacg cagattgaaa ccgcggaaag
    15541 ggagcaatcc agcaaatacc gaaaggggac gggcgaagaa tatcatccat gccgacaaaa
    15601 tgagcgccgg aatggcaata ggcagcagat cgcttgggtt aaccaatagc cccagcacca
    15661 ggaacatggc gatttgcgcc agccaggcga ggccgtcgaa attttgcagg atgccgtagc
    15721 ggttgcgaat cggccgatta cccagcagaa aaccgcacag ataaacagcc agaataccgc
    15781 tgccttccag cgccgtggtt aaagcaaaaa ttaaaatacc gccgcttaat gccagcaatg
    15841 gatataatcc ggcaggcagg gcgatgcgat taatcatttg cagcagtaaa taaccaccgc
    15901 caagaccgat gacaatcccg aggccaaatt gctgcagaat atcgaccacg aacatccaac
    15961 tgacactgct ttcatgctgc tggatcattg caatcagggt aatcgtcaga aagaccgcca
    16021 ttggatcatt actgccggat tcaatttcca gcgtcgagcc aacacgttcg ttaagcccct
    16081 taccacccag cagagaaaag accgctgcag catcggtgga gccgacgata gcgccgatta
    16141 ataagccttc aatcaaatca agattaaaca gccacgccgc catcatgccg gttaaaccag
    16201 aggtgataag tacgcccagc gtcgccagcg acagcgccgg tcctaacgcc acacgaaagg
    16261 aactggcctg agtgcgcatc ccgccgtcga gcaaaataat cgccagcgcc aggttactta
    16321 ccatgtaggc gaaggggtaa ttatcaaacg ggataccgcc gacgccatcg actcccgcca
    16381 gcatgccgat cgccagaaaa ataaccagaa taggaatgcc aagacgggaa gaaaatgaac
    16441 taagtaatat actgctggtg acaaggatgg atcctaagat gaaaaggcta attattgttg
    16501 tggcatccaa cggtcggtta ctcctgatta cgctgtctct tatataaacc ctaccatatt
    16561 agcggcagag acagcgtttt acttagccct gaagcgtgat ttttttacat tttaagaaca
    16621 ggatgaccgc taatcgtcaa ctgagtgcct tcccgggtgt tattcagaat ggcatgtgcg
    16681 cccaacggca gcgtaaccgt gcgttgttca tgaccaaaat cgaggccggt aatgagtgga
    16741 atcgacaggc gggaacgcaa aaacgcgtac actgactcca ggttgtaacc tgcgtcataa
    16801 tcattgggcg tgctgccgct aaagctaccg agaataatcg ccttctgacg tggcaaaatt
    16861 cccgcatgat agagctgcaa cagcatacgt tcgacgcgga aagggtgctc gttaatatct
    16921 tccagcacca gaataccgtt ctcaattttt ggcatccacg gtgtaccaat cagtgaaatc
    16981 agcatcgcaa gattgcctcc ccacaacgtg ccttcggtct gacatgtcgg gccttcccct
    17041 tgccattcaa tggtgaaggt ttcattgcgt aacgccagcc agaaatggtg ctcggtaaag
    17101 gcgttcagtt catccgcacc aaagtttgct accagcatgg ggccgctaaa ggtgatgaca
    17161 ttgccttgcg ccagaagacc gcactgaatg gcggtaaaat cgctatgtcc gcaaatgagt
    17221 aacgggtcat gttgttggcg agccaccagt gcctgccagt caatatctgc cagtaaacga
    17281 ctggcaccgt aaccgccgcg tacagccagc acgatggtgt tgggggtagt cagtctggca
    17341 agggaattga gatcttccag acgctccgtt tccgtacccg caaaacgctc acaacgacgg
    17401 gcaataacct cgacgttatt gacttgatgc cccgcgtcgg taaggcgctg gataccacgc
    17461 agcgcggcgt gctgtttaat gctgtaaccc gatggggcaa ttaagtgaaa cagagacatg
    17521 gcaattcctt gctgacaaca gaaacgaaat gtatatcatg ccgcttaggt gtgccgttgt
    17581 cacctcaacg gcgattccag gctataagga tagaagaagt gaaattgaga tggtttgcct
    17641 ttttgattgt gttattagcg ggttgttcat caaagcatga ctatacgaac ccgccgtgga
    17701 acgcgaaagt tccggtgcaa cgtgcgatgc agtggatgcc aataagccag aaagccggtg
    17761 cagcctgggg cgtcgatcca caattgatca cggcgattat cgctatcgaa tcgggtggta
    17821 atccgaacgc ggtgagtaaa tcgaatgcca ttggtttgat gcagttaaaa gcttcaacct
    17881 ccggacgtga tgtttatcgc cgtatgggct ggagtggtga gccgacgacc agcgagctga
    17941 aaaatccaga gcgtaatatt tcaatggggg cggcttacct gaatattctg gaaaccggcc
    18001 cgctggcagg cattgaagat ccgaaggtac tgcaatatgc gctggtggtg tcatacgcta
    18061 acggggcagg tgcgctgcta cggactttct cgtcagatcg gaaaaaggcg atcagcaaaa
    18121 tcaacgattt ggatgctgac gagttcctcg atcacgtagc gcgaaatcat cctgcgccgc
    18181 aggctccgcg ctatatctac aaacttgagc aggcactgga cgcgatgtaa atcagtcgcg
    18241 cactttgtcc gctttttccc gggcttctcg ctcgagagag aaaataatcc gctgtaattg
    18301 ccgctccacc gtcgggctga cgttaagaaa acggaagctc agacggggag tggtgatggt
    18361 ttcattcttg ccatcaatca ctttgcgctc gctggtggag attaactggg cgtcaaagtg
    18421 aaaaacaccc cattgcccca tgttgacttc aatttgagcg aagcgcatac cttcttgtaa
    18481 ttcggcaggc tttgctgttt ccagtaatgc gcccatgccg cctaacgaca aatcatacaa
    18541 gcggaaacgt aacgtactgt tatccgccag tttggtctgg caaaaataag gcggatggag
    18601 tggggcggag atgcggaaat atcggcgtcg ttgtacaaac cataaggtgg gaggcggtac
    18661 ggtaataaat gccggaagct gcaagtattc actctgctgt agttgttcaa cagtaaactc
    18721 gactttcgca ccctgagttt cggcggtaat ggtaatgtgc tgtgccttta gcacggcgat
    18781 gttgtcttcg gcttgactgc cgaaatccag caccagttta tccggggtta ttgccagtat
    18841 tttgctgatc agctgcccgc cattccaact gagacgcaaa ggaattgcgg ctttgtgcaa
    18901 atcgcgtaac acgcccagga cggctaacgg attttgtttc aggaactgct catggtaatg
    18961 actcacgcgg aaaaactcct gactgacaaa ctgtctttgg tttatcggtc atagttaaca
    19021 aaacttaata caaatgcgtg aatatttttt acatgttgtt cttaaatcag ccgacaaacg
    19081 cccagcatcg atcccgccct tgcctatact tagagcgttg acgtaagcat ttcttgcgac
    19141 cgattcatcg aaacgagggc ctgaacatgg gaattattgc ctggattatt tttggcctga
    19201 tagccggcat tatcgccaag ctaatcatgc cggggcgtga tggtggtgga tttttcctga
    19261 cctgtattct cgggatagtc ggtgcggtgg tcggcggctg gctggcgacc atgtttggca
    19321 ttggcggctc catcagcggt tttaatttgc acagcttcct agtggcggtg gtgggcgcta
    19381 tcctggtact gggcgtattc cgcctcctgc gaagagaata agattttcat aaggcggata
    19441 gcgatacaga tgccgctatc cgctttcaca tcagaacgta tactcgacac ctgcccagaa
    19501 attgcgacct tcttcaataa acccttcact gtaggcatat tgggtatcaa acaggttatt
    19561 gaccgacgca ttgacgctga agccatgacc taaggtgtaa tcggctcgaa tgtgggtcac
    19621 cgcaaaaccg gcggcttttt gtgaaccgtc actgttgctg tagctggagg aacgcgcctc
    19681 ttccgacagc gttacgctta acggctccca tggtttgagt gtcatccatg cggtcattgt
    19741 ctgcgttgga agatcggtta tcttgccgat gtctttgcgt ttggcgtcag cgtggatcag
    19801 agcgtagctc aatcctacat ccagtatatt gctgattttc cccttaatac cggcatccag
    19861 accgctgtaa tccaccgtgc cgctgttctg attttgaatg gtatcggcat tgatattgtg
    19921 cgagaggatg gcatcactca cccggttata gtaaacgctg acctcaaatc cccagtcgcg
    19981 cgtgaaggca ccattccagg ttaaatccac cccgcgagcg cgttccggct tgagctgcgg
    20041 gttaacaatc gctatctggt tatacgcagg tttggatgtg gtatagcgtt ctttcagcgt
    20101 cggaaagcgt gtgcggtcat agtacgaaag cgccagagtg tcttcattgg caaagtggta
    20161 tttccccatc acctgccagt taaaagctga ctgattgttg tcgtcataat gggtgatgct
    20221 gccatctttc tcgtgttttt tcgcttctac gctatcgcgc cagtcatagc tgattcccgc
    20281 cacgacatcg acattatcag cagcagccca ttgatattca ctggcgagcg accaggtacg
    20341 atcttcatag cgatcgtaag cggcgtgcgg cgcacctttt tcccggtgta cgtcatcttt
    20401 ccagttaacg gcaaacgaca gcagatcgtt ttcacgcaca tcggctgcca gttgtaatcc
    20461 ggcaccgtcg ctgtaatcgg aataatggct gtagctgcct tttttatttt tcaaatcagc
    20521 cagcgagttg tacatcatca gcgtattttc aaaggtgtcg cgatacagcc gacttttcag
    20581 ggtaaaacgg tcgtttagtt gggtcgctcc ctgataataa aaactttctt tgtcatactc
    20641 tggccactgc cagtagcgtg atttttgacc acaatttccg ctgtatggcg ggttatcttt
    20701 ttcaccatcc tgcttaatgt aagtcagcgt gtattcatcg ttttcgcgcg gtgtaaaacc
    20761 gagcttcaca atgccgcgtt tatcatcagc cgatgaatta atcatcttgc cgtgtttgcc
    20821 tgcaatatca ttatttacac catgcggcag gccgagaaaa tcctgcttta gctggctacc
    20881 gctgacttgc aaatacccaa gatcgctgct ggcggcaaat gaagcatgca tatcgtaggc
    20941 attgtcctga ctacggctcc atccctggcg atatcccaga cttgcttctt cagggtggtg
    21001 atattaatgg ctccgcccat ctgattaggc ccatgaagca gcgacgaata ccctttggaa
    21061 acttcgactg cccccagatt gttggtcaga acccgcgcca gatcgaggtt gccgtcatag
    21121 ggaacataaa tgggcacacc gtcgaaatag accggcacct gccgactatc aaagccacga
    21181 acttttacct gttcttcgtt gcggctgcct gacttttgca gcaccacgcc ggggacgaca
    21241 cttaatgcct gagcgacatt ctgtttatcc aaggctttca tggtgggttg atcgagaacg
    21301 gtggtcgtcg tcgatgatac cggactggac catacggtca gcgtgtcgct ctcctgagcc
    21361 gctacggcct gctgggtaaa ggcaagtgat aatgctgtgc agagataacg ttttttgagt
    21421 ctcatagaat aaattccgtt taacaaagaa ttaaatagag gctgtgggtt gcagactgac
    21481 tttaatgtca gctggtgccg cgtaataagg cgcggaggta ataaaaaggc gaatgccgca
    21541 gtcgaggtaa tttttgagtg tcgtcagatt aattccgccg gtcagagcca gcgtgcagtg
    21601 aggagccagc gacggcgcta tttgcgcaat ttcagttgcc tgttgcggac taaatttgtc
    21661 gagctgaagt acgtctggtt gtgcgcgtaa cgcggcgata gcctctttcg gcgtgtcggc
    21721 ttcaacaaca attttcttct cgggtgcgtg gcgacgtaac tgagcgattg cgcctgacca
    21781 gccctgattg tcatgaagaa aatggcggtg gttggcaaac aataatatgg tttccgcaca
    21841 tccggcacga tgaattagtc ctccggcagc cagaattgcc tgcgaagcca gtaagcgggt
    21901 gcccggaatt gcttttcggg tgcaggcgat attgccgtca ggataacgtt cacgaagcaa
    21961 tgccagcatt tgagcgagat aatcagaaac accgcaactc cactccagca cattctggac
    22021 tgccttccat ccctgatgaa gtgctgcggc attaccctgc gcacggatta gccgttgacc
    22081 cgcgttcgct tgtgaaccgt cgctgaccgc gtcatcaata gttaatccca gcgtagttaa
    22141 catcttacac gcgacagaaa cgccgctgac gcagccgccc tgacggtgaa aaaacgctat
    22201 atagccaggc tggtgtccaa tatttaatgc tcgcgtggtc aggtcacctc cctggatatc
    22261 ttccagcagt aacgcatcga tttgtgcctg agaaaggaaa atcatcacag tgcctcctgc
    22321 ggtacgctat cccaccagat gagcgcccag tcgcggctgg caggggcgag ggcacgagca
    22381 tcttgttgtt gataccagtg atataaccgc tcacgctcat cgtcgttaag cgcgccaaga
    22441 ctccagctga cgttctgctc aaaacgttcc caggtgctgt tatcctgttg gcaattttgt
    22501 ccacggataa aatcaacgtg ggcataaatt cccatctgat acaggacgtt gagcgcaaag
    22561 atatagttgg gtaattcgat cacttcccga ccagccgccc gctgaatggc tggagagacg
    22621 aacgaggtgc tgaccagatg agttgtatag acgcgaagcc gtgcctgatt attcagtttg
    22681 ctcattgcat ggcgcatatc agccaccaga gtggagcggg aagccacggc aatatcgcag
    22741 cgtggcaggt cactccagtc ctcttcccag gcgcgctgga tccagtggac attgtcagcc
    22801 ttcagtgcgg cggcgcggcg ggcggcaacg ttcagcatcc cctgactgta atcaacgcca
    22861 taaaccgtag tgagtttgtc agccagcgcc agcgatacgg tgcctggtcc gcagcccata
    22921 tcgaataagg tctgagcgcc ttgcaggtca attttagcga tcagttgttg caggtagctg
    22981 tccgttggcg aggcgcaatt ttccgccatt ttctccgccc gttgatccca gtgatcgggc
    23041 gttttttctg tgcggtgagc cagtttgagt tgctgcagat aaaggtctgc aaaatcaata
    23101 tcatcaatga gcatgattgt tccttagcgg ctaattgcag agaggtgatg atgaatttgg
    23161 tcggcagaaa cccgatacag ggccgccagt ttgttcgttg ttaactgttc ggtcggtaag
    23221 ccttgtgtta ctcgtccatc tggctctacc tggataatgc tgtcggcaat ggcgttggca
    23281 tggagcggat ggtgagtcga cattaacatt gtcatgccgt tatttttgag ctgcgccagc
    23341 gtgtccagaa gttggatctg atgaccgaaa tcgaggctgg aggcgggctc atccagtaac
    23401 aacaagcgcg gttgttggac gagtgcacgg gcgattaaag ccagctgacg ttcaccgccg
    23461 ctaagcgtat tccagcgtcg tgtcgccaga tgcaaaatgc ccagcttttc cagttgctcc
    23521 gtggctttca ggcgttcttg cttaccggga acggaaaaag cgcctatggt gggggccagg
    23581 cccatcaaca ccatatccag cacactgaaa gcaaaaatac cgtcgtgcgc ctgcggcacc
    23641 caggcaatag cctgtgcgcg ctggcgatgg ctgagtgtct gcacagggag gctatccagt
    23701 aggatctcgc cttttaataa cggtataacg ccaagaatgg agcgcatgag tgtggttttg
    23761 ccgcaaccat tcgcgccaag cagacaacaa attgttcctt gcgacagggt gaaggacacg
    23821 tcgcgtagca ctggatggcg ggagtaaccc agagacgcat gacgaacggc aagcaaagtc
    23881 actggcgacc tccgcgtaac aacaaaagca ggaaaaacgg tgcgccaaca aaagcggtca
    23941 gaatgccaag tgggatttct gttgttccaa tgctgcgcgc cagcgtatcg gtaagcagga
    24001 gcagaatagc gccagtacac attgccatag gcaacagctg ctgatggtta tgaccggtca
    24061 acaagcgggc aatgtgcgga acgaccagac cgatccaacc aattatgccc gctattgcta
    24121 ccgtgctggc ggtgattagt gtggcgcaga caatcaatcc aaagcgcaga cgcgtcacgt
    24181 tcagtcccag cgaacgggct tcgtcgtcgg aaagcgtgag taaattcatg cgccagcgga
    24241 gaaaaaacag gggcaaggag cctatgagaa tgatcggtgc agcatagcag agatcgcgta
    24301 aggtcacggt ggacagtccc cccagcaacc agaaggtaat tgacggtaac tgggtgtaag
    24361 gatcggccag cgttttaatt ggcgatatcc ccgctccgca caacgttcct aacgcaatgc
    24421 ctaccagcac cagggttaag atcggatcgt ggcgggtcac ccgacgcgta atcaaccaga
    24481 cgcccgcgac caccatcagc tcgccgcaaa atgcgtacag ctggatgtaa acaatgggta
    24541 atccccacag gattgccgcg caggcaccaa gtccggcacc ggcggagacg cctaaaatat
    24601 ccggtgaaac taagggatta cgcaacattc cctgataggt ggttccggct cctgccagcg
    24661 ctgcgcccag taacagcgca gcaagaatac gcggcacgcg gatttgccag aagacaattt
    24721 gttcctgcac agacacattt tcttgacgaa gaattagcgc cagcagttgc tgcatatcga
    24781 gatgataagc cccacttacg gcggcaaaag tgatactgat agctgccagc agcaaaatac
    24841 atccgtttac gattctcatt agctcgccac caatttttga tagtcggcat cactgagtga
    24901 acaatgccag aacagttggg cgtaatgctg catgtcactt ttaaactggc gattgatggc
    24961 gggatccagc caggcgtgaa gtctgcgtaa tcccagaaga cggttgattc ccggcggggc
    25021 atcgagccag ccaaagggca agccacttaa aaataagatg cgttgttccg caacggcttt
    25081 cacgccctgc cagagaggat cacgacgaat aaaatctgca gtaacggcct cctgaaccag
    25141 aataatatcc ggctgccagc gcaggagatt ttccatggaa acctgtgtca ggccgtggcg
    25201 atcggctatt tgcgcgacgt tgtgcaaacc taataattcc gccgcctcgg tatgcaacga
    25261 accctgtaag cccgtttcca gccctcgggg accgcgcgca gcataaaagc ggaggttagc
    25321 ggcgggtgag gtggcgaatg cctgagcttc accaacgaag tggctggcga gattggcttg
    25381 ttctgcggcg cggtgctctt cgcctaacgt tttgccaagc gttgttaact gttctgctga
    25441 ttgcgccagt tttccgttaa gcaataacca ggggatttgt gtctgttcgc taacctgccg
    25501 tgcctgggag atcaaggttt catccgtatt gccgcaatca acgaccaaat cgggatgtaa
    25561 cgccataagc ccttccagcg atagtgtgct ggcgcgcccg gcgagtcgtc ccagcctggg
    25621 taactggcga atgtgctctg gcaatggaat taatgcctga cgggcaaaat caaaagagga
    25681 aaaaccgacc attttttctg gcgcgacagc cagcaatagc aaatcggcgg gcgcgcctgc
    25741 gctgacgatg cgtgtaatgt tttgggcttc gggaagagat tgcgaagcaa aacgattaac
    25801 tgtttgtgaa aaggacggta gagactgcag gagcagggta gatgcaagtg cctgcgcaaa
    25861 cgtccgtctg gttatgggca tggtgaacat
//
LOCUS       DAJTYF010000045        25655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTYF010000045 DAJTYF010000000
VERSION     DAJTYF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.045"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..95)
                     /locus_tag="OO590_002023"
     CDS             complement(<1..95)
                     /locus_tag="OO590_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HCR6637395.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYA"
     gene            complement(165..1154)
                     /gene="araF"
                     /locus_tag="OO590_002024"
     CDS             complement(165..1154)
                     /gene="araF"
                     /locus_tag="OO590_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR6637396.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1951..2454
                     /locus_tag="OO590_002025"
     CDS             1951..2454
                     /locus_tag="OO590_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6637397.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2534..2785)
                     /locus_tag="OO590_002026"
     CDS             complement(2534..2785)
                     /locus_tag="OO590_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6637398.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2900..2986)
                     /gene="azuC"
                     /locus_tag="OO590_002027"
     CDS             complement(2900..2986)
                     /gene="azuC"
                     /locus_tag="OO590_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6637399.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3249..3572
                     /locus_tag="OO590_002028"
     CDS             3249..3572
                     /locus_tag="OO590_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6637400.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLH
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3743..4240
                     /gene="ftnA"
                     /locus_tag="OO590_002029"
     CDS             3743..4240
                     /gene="ftnA"
                     /locus_tag="OO590_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6637401.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4277..4516)
                     /locus_tag="OO590_002030"
     CDS             complement(4277..4516)
                     /locus_tag="OO590_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6637402.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4706..5917
                     /gene="tyrP"
                     /locus_tag="OO590_002031"
     CDS             4706..5917
                     /gene="tyrP"
                     /locus_tag="OO590_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6637403.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSMPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5979..6644)
                     /gene="yecA"
                     /locus_tag="OO590_002032"
     CDS             complement(5979..6644)
                     /gene="yecA"
                     /locus_tag="OO590_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6637404.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6840..6926)
                     /locus_tag="OO590_002033"
     tRNA            complement(6840..6926)
                     /locus_tag="OO590_002033"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6890..6892),aa:Leu,seq:taa)
     gene            complement(6939..7012)
                     /locus_tag="OO590_002034"
     tRNA            complement(6939..7012)
                     /locus_tag="OO590_002034"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6978..6980),aa:Cys,seq:gca)
     gene            complement(7294..7842)
                     /gene="pgsA"
                     /locus_tag="OO590_002035"
     CDS             complement(7294..7842)
                     /gene="pgsA"
                     /locus_tag="OO590_002035"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6637405.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7899..9731)
                     /gene="uvrC"
                     /locus_tag="OO590_002036"
     CDS             complement(7899..9731)
                     /gene="uvrC"
                     /locus_tag="OO590_002036"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6637406.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9728..10384)
                     /gene="uvrY"
                     /locus_tag="OO590_002037"
     CDS             complement(9728..10384)
                     /gene="uvrY"
                     /locus_tag="OO590_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6637407.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10680..10856
                     /locus_tag="OO590_002038"
     CDS             10680..10856
                     /locus_tag="OO590_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637408.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10843..11067
                     /locus_tag="OO590_002039"
     CDS             10843..11067
                     /locus_tag="OO590_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6637409.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11100..11720)
                     /gene="sdiA"
                     /locus_tag="OO590_002040"
                     /pseudo
     CDS             complement(11100..11720)
                     /gene="sdiA"
                     /locus_tag="OO590_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11950..12702)
                     /gene="yecC"
                     /locus_tag="OO590_002041"
     CDS             complement(11950..12702)
                     /gene="yecC"
                     /locus_tag="OO590_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6637410.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12699..13367)
                     /gene="tcyL"
                     /locus_tag="OO590_002042"
     CDS             complement(12699..13367)
                     /gene="tcyL"
                     /locus_tag="OO590_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6637411.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13382..14368)
                     /gene="dcyD"
                     /locus_tag="OO590_002043"
     CDS             complement(13382..14368)
                     /gene="dcyD"
                     /locus_tag="OO590_002043"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6637412.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14473..15273)
                     /gene="tcyJ"
                     /locus_tag="OO590_002044"
     CDS             complement(14473..15273)
                     /gene="tcyJ"
                     /locus_tag="OO590_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6637413.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15361..15912)
                     /gene="fliZ"
                     /locus_tag="OO590_002045"
     CDS             complement(15361..15912)
                     /gene="fliZ"
                     /locus_tag="OO590_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6637414.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15958..16677)
                     /gene="fliA"
                     /locus_tag="OO590_002046"
     CDS             complement(15958..16677)
                     /gene="fliA"
                     /locus_tag="OO590_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR6637415.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVCHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16842..18494)
                     /gene="fliC"
                     /locus_tag="OO590_002047"
     CDS             complement(16842..18494)
                     /gene="fliC"
                     /locus_tag="OO590_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6637416.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTVGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18760..20166
                     /gene="fliD"
                     /locus_tag="OO590_002048"
     CDS             18760..20166
                     /gene="fliD"
                     /locus_tag="OO590_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6637417.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20191..20601
                     /gene="fliS"
                     /locus_tag="OO590_002049"
     CDS             20191..20601
                     /gene="fliS"
                     /locus_tag="OO590_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6637418.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20601..20966
                     /gene="fliT"
                     /locus_tag="OO590_002050"
     CDS             20601..20966
                     /gene="fliT"
                     /locus_tag="OO590_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6637419.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21044..22531
                     /gene="amyA"
                     /locus_tag="OO590_002051"
     CDS             21044..22531
                     /gene="amyA"
                     /locus_tag="OO590_002051"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6637420.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDCIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22565..22978)
                     /locus_tag="OO590_002052"
     CDS             complement(22565..22978)
                     /locus_tag="OO590_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6637421.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23165..24370
                     /gene="yedE"
                     /locus_tag="OO590_002053"
     CDS             23165..24370
                     /gene="yedE"
                     /locus_tag="OO590_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6637422.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24367..24600
                     /gene="yedF"
                     /locus_tag="OO590_002054"
     CDS             24367..24600
                     /gene="yedF"
                     /locus_tag="OO590_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6637423.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24707..25377
                     /locus_tag="OO590_002055"
                     /pseudo
     CDS             24707..25377
                     /locus_tag="OO590_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25417..>25655)
                     /locus_tag="OO590_002056"
     CDS             complement(25417..>25655)
                     /locus_tag="OO590_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6637424.1"
                     /translation="GGPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGAR
                     NILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         6375 a         6556 c         6099 g         6625 t
ORIGIN      
        1 gcatagcagt caaaactaat atccgtcagc gccttaacgc cgggaaacgt tttaccgatg
       61 ccgcgaaatg agagatacgg ggtagactgt tgcataacgt ctccgtgaat cactggtcgt
      121 tcacaccccc tctgcatcat gcagagggga atttttccgg caaattactt accgcctaaa
      181 ccttttttct ccagttcttc tttaaagttg tcacgcgtga tcagtaccac gtctgtaact
      241 tcggtaaatt ttggcgtttc aacgtctttt gctacccagt tgtaaagcat ttcgctggat
      301 ttatagccat gtacgtccgg gcttggcagc agggaaccgt agaagccggt tgcctgtgct
      361 ttagacagtt cgcttactgc atccacaccg ttaataccaa taccgatgat atcggctgct
      421 ttaaagccct gaccttcggt tgcacgtacg ccgccaagta cggtgctgtc gttcataccg
      481 acgatcagcc aatgtttaac ttccggatgt tgaaccagca ttgagttggc agcgtcaaat
      541 gcccccggga tgtcgttaga tttggtaggt acctgataaa tttgtttttc cgggaatcct
      601 gcagctttca gtgcgtccat agaacccgtt gtacggcggc gggcggtatc cagttcgttg
      661 gcggtaatcg ccatcactgc gctttctttg acatcccagc cacgtttctg catctcttta
      721 tacagttcct ggccctgacg ttcgccaatt ttagtcgccg ccatcatcac cagaggaacg
      781 gtatccattg gcttaccttt ggcgttaaca aactggtcat ccactgcaat gactttcata
      841 tcgtagccac gcgctttcgc gacgatggca gagccgagtt tcgggtccgg agtacaaata
      901 acgaaacctt ttgcgccact ggctgccagg ctgtcgatcg cgttcaatgt tttttcgcca
      961 tccggcacgg caatcttaat aacctcaaac cctaaatcct tcccggcttt atcggcaaac
     1021 ttccattcgg tctggaacca cggctcttcc ggttgcttca ccagaaaacc gagcttcagg
     1081 ttctccgcca tagcggattg tgacataacg gctgccagac caatggctgc cagggcttta
     1141 gtaaatttgt gcatggttct ctccagcttt agtgtcgttt tgtgtagggc aaaaacgaat
     1201 gacattcgtt aaattaatcg gaaaacaaag cattaccttt tatctaaaag ataagtgacc
     1261 gtgttgacat agttttagcg agaaattaat tctccatagg agagcaatat cacatcgcag
     1321 aattacagtg agaacgtgca taaatttagc gggaaaagac ataaggaaaa gccaatttgt
     1381 cagacaaatt gttgaatgca cagcagatta atccataaga ttagcctgga aatccttgtt
     1441 gtcttcggta cccatgcggg atgtcttctt tttaaccagt caataggccg cattacctgg
     1501 cgttgagttt ttgaaatggt gtaataacca caactcaaag atgtggaaaa tgcgcgtcat
     1561 tcatttcgcc attaattatc actgtgctca ttaattaaca gaacacgtat aatgagagcc
     1621 atctcgcaaa aatgaaaaaa cgttttataa aatcatcact tcatcatgaa ttcaaattca
     1681 tcgattaata tcaacaagat acaaaaagca ctatcattaa aattcattgc atttacattg
     1741 atttcaccaa tgaaatgtaa aaatatataa acttgatgat ttaagcattt tcttataacc
     1801 gttcagacgt tattcttatt tcagatcatc gtcagaattg actccacgat cacatttcgg
     1861 accggcagaa aggaattatt ctgcaaacag taattatggt gttttgattt atcttgcacc
     1921 tctccacttc tggatataag gatattaggt atggcaaccg ctggaatgct tctcaaactc
     1981 aactctcaaa tgaaccgcga gttttacgca tccaatctct accttcacct gagtaactgg
     2041 tgttctgaac agagtctgaa cggcaccgcc actttccttc gcgcccaggc acagagtaat
     2101 gtgacccaaa tgatgcgcat gtttaacttt atgaagagtg tcggcgctac ccccatcgtt
     2161 aaagccattg atgttccagg tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg
     2221 atggaagaat acgagcaacg ttcaagcacg ctggcgcagt tagccgatga agcgaaagaa
     2281 ctgaatgatg attcaaccgt caatttcctg cgcgatctgg aaaaagaaca gcagcatgat
     2341 ggcctgttgc tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc
     2401 cctgtgcaga ccgaccaaca tgttctgaat gtcgtgtcac accagctgca ttgatcatac
     2461 atcggcgcta atacattgcg ccgatgaagt ttttgagaaa ccgctgcctc atctgtttga
     2521 agcagcggtt tttttaatgg gattcaccct gtggggtaaa cttgagttca atcagcgcga
     2581 tggctttttg gattgcccgc atggtgaccg ggtctgcggc agcgggatga tgagtaaagt
     2641 cgatattctt cagctgactg gacatttttt cacgaacttc aacgggcgcg attacatcga
     2701 gaacatccag aatttgtttg ataaccaact ggcaagctac cacatcagaa accagttcct
     2761 gatcggcatt cagcggctgg gacatcgtaa actcctgata gcattttgaa agccgttata
     2821 gtagcggctt cacatcttca gcgatagtca catccaccgt catcaggaca caaaaaaacc
     2881 tgccggagca ggttttttgt tatcggaaca tattgcctgg cggtacgtct ttgaacgtct
     2941 tgcaatagtt attgaacata cttttcagga ttttgcgcag tttcatcgcg gcactccgac
     3001 catttgttat acaggtgtta ttgtctttgc gcttataata tgacaaccat cacaaaaatc
     3061 aatctttatg tgatacaaat cacataaata cccctttaat gttataaaaa tgataatcaa
     3121 aaaacagccc ccctatttct gacacctaca gatggcaaga aatagcgcct gccaggcatc
     3181 ttttccggcc attgtcgcag cgctgtaacg cgtaaaatag tgctttctct tactcttctg
     3241 gctggaccat gagacttctg attctgactc tttcattaat aacgctggcg gggtgtacgg
     3301 ttactcgcca ggcccatgtg agcgaagttg acgcggcaac aggtattgta cggttggttt
     3361 atgaccaggc ttttttgcat catgcccata ccgatcgtta tgtcagtcgc gggatcgccg
     3421 atcgcgcatg ccagcaggaa ggctataccc acgcgatccc ctttggtcag ccagtaggca
     3481 actgtagcct ttttgcgggt tccttatgtt tgaatacgga attcactttg tcatatcagt
     3541 gccatcattc cgccttccct gtctttctct aaatatttta atcagcgagg ggatcttcgc
     3601 tgattaaaga aatagcggta atgcgtttta ttcccattcg tatttttaat aattgaagtt
     3661 tatattttac cttttgcaaa taataaaata acaaattata gtgacgccac agcaacaaat
     3721 ataacctttg tggagcacta tcatgctgaa accagaaatg attgaaaaac ttaatgagca
     3781 gatgaacctg gaactgtact cttcactgct ttatcagcaa atgagcgcct ggtgcagcta
     3841 tcataccttc gaaggtgctg ccgcgttcct gcgccgtcac gcccaggaag agatgacgca
     3901 tatgcagcgt ctgtttgatt acctgactga taccggcaat ttaccgcgta ttaataccgt
     3961 tgaatctccg tttgctgaat attcctcact tgatgaatta ttccaggaaa cctataaaca
     4021 cgaacaatta atcacccaga aaattaacga actggctcat gctgcaatga ccaatcagga
     4081 ctacccaaca tttaatttcc tgcagtggta tgtttctgag cagcatgaag aagagaaact
     4141 gttcaaatcg attattgata aattaagcct ggcaggcaaa agcggcgaag gtctgtattt
     4201 tatcgacaaa gaactctcta ccctcgacac acaaaactaa tgctctccgg cggcaggttt
     4261 ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg atcggtcgaa aaaccttctt
     4321 ctgaaggtat aaatttgcct tttgctgcca gaaacgctac cagctctccc gctgtcatcc
     4381 cttctgccga gcacgtgtga aaacgtgcct gttcaccaaa acgcgcttta atcgcagctt
     4441 ccagactggc atgcgtatat tgctcgcctg attcaatcat catatttaac acttcatgac
     4501 cgtgaataga gtccatcgtt cctcctcaaa aaaagcctag cgtagcgatt accgcttatg
     4561 aagactttgc gccagcgcag gactgaatgc ttttattgta catttatatt tacacaatat
     4621 gtaacgtcga tttgacgaag cagccgttat gccttaacct gcgccgcaga tatcactcat
     4681 aaagatcgtc aggacagaag aaagcgtgaa aaacagaacc ctgggaagtg tttttatcgt
     4741 ggcgggaacc acaattggcg caggcatgct ggcaatgccg ctggctgcgg ccggtgttgg
     4801 ttttagcgtt acgttaatct tgttgattgg gctttgggcg ttgatgtgct acacggcgct
     4861 attactgctg gaggtgtacc agcatgttcc ggcagatacc ggtctgggca cgctggcaaa
     4921 acgctatctg ggacgctacg gtcaatggct gacgggcttc agtatgatgt tcttaatgta
     4981 tgctctgact gcagcataca tcagcggtgc cggtgaattg ttggcctcca gcatcagcga
     5041 ctggacaggt atttcaatgt cggcaaccgc tggcgtgctg ttgttcactt ttgttgccgg
     5101 tggcgtggtt tgtgttggaa catcgctggt cgatttattt aaccgttttc tgttcagcgc
     5161 caaaattatt tttctggtgg tgatgctggt attgctgcta ccgcatattc acaaagtgaa
     5221 tcttttaacc ctgccgttgc aacaggggct ggcactgtct gcaatcccgg tgatttttac
     5281 gtcgtttggt tttcacggta gcatgccgag tattgtcagc tatatggatg gcaacattcg
     5341 taagctacgc tgggtgttta taatcggtag tgccatcccc ctggtggcat atattttctg
     5401 gcaggtggcg acgcttggca gcattgattc aacaaccttt atgggattgc tggctaatca
     5461 tgctggatta aacgggctgt tacaggcgtt acgcgaaatg gtgccctctc cgcatgttga
     5521 gctggcggtg catttatttg ctgatttagc cctcgccacg tcatttctcg gcgttgcgtt
     5581 aggcttattt gattatctgg ctgatttgtt tcagcgttca aataccgttg gtggacgatt
     5641 gcaaactggt gcaattacct ttctgccgcc gttggcgttt gcactgtttt atccacgagg
     5701 atttgtgatg gcgctgggtt acgccggtgt ggcgctggcg gtactggcat tgattatccc
     5761 ttcactattg acctggcaaa gcagaaagca caatcctcag gcgggttacc gggtcaaagg
     5821 tggtcgtccg gcgctggtgg tggtgtttct ctgtggtatt gctgtgattg gcgtgcaatt
     5881 tttgattgcg gcagggttgt taccagaagt ggggtgatca gatagcctca aattccttat
     5941 tgggtgccag aattaacgct gacacccaat gtgacctctt aatgcaggca gcactgctta
     6001 aatttcttac cactgccgca cggacaagga tcgttacgcc ccggtttctc ttctgctttg
     6061 atcggttgct gaacagcttt ttcctgcgga tgcgccatcc agtatgcatg taaatcaagc
     6121 gccgccagtc gaatggcatc tacgctctct tcaaacgctt ctggcgacat tttctctacc
     6181 cgctcgaagt tttcctcagt accgtgcagc gcaatcgcct ccagcgctgg ttttaaagaa
     6241 tcgggcaacg ttgaccagtc agaaagtgcc acgccccgca tatagccaaa gcaccactcc
     6301 tcaacaatcg tcagctcgct gccatcaact tctcgcaagc cgaataacgg ctcaaactgc
     6361 tccgggaatt cgttcagacg ctctgcggta tcggccatat gttgaaaagc caggttcata
     6421 aagcgcgtca tctctttctc tgacgcccaa cgcggcacat agtcagcccc accccacacg
     6481 gcaaccagcc actgttctgg ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa
     6541 ccgtccagct ccgccacatc aaggatggcg tggtcagtgt tgtatttggt cagaatatcg
     6601 tccagccatt ccaactcact ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg
     6661 atctcaactc gccagcacct atcttacatg ccggtccgta tcagagatac tttttgagtg
     6721 gctttgccgg tgattaaaaa ttaaggaggg tgtaacgaca agttgcaggc acaaaaaaac
     6781 cacccgaagg tggtttcacg acactgctta ttactttgat tttattctta tctttcccat
     6841 ggtacccgga gcgggacttg aacccgcaca gcgcgaacgc cgagggattt taaatccctt
     6901 gtgtctaccg attccaccat ccgggctcgg gaagaaagtg gaggcgcgtt ccggagtcga
     6961 accggactag acggatttgc aatccgctac ataaccgctt tgttaacgcg ccaaattctt
     7021 caggcctttc agccagacat ccgcttgacg ccgatgtctt ttaaactgga gcgggaaacg
     7081 agactcgaac tcgagacccc gaccttggca aggtcatgct ctaccaactg agctattccc
     7141 gtattcatca agcaatcagt taatcacttg attttattat cgtctggcaa tcagtgccgc
     7201 cgttcgatgc gttgcattct acttacctgg cgcgatgagt caacgatatt tttcaccact
     7261 tttgatcgtt tgctgaaaat tacgccgaaa cgatcactga tcaagcaaat ctgcacgcgc
     7321 agcgctcaaa tattgcaaca ttgaccacag agtcagtacc gcagccacaa agaaaagtgc
     7381 aataccggcg tactcaaccc aaatgttcgg acgccacagc agccaggcca acgccaccat
     7441 ctgggcagtg gttttcactt tcccaatcca ggagacggcc acgctactgc gtttacccaa
     7501 ctccgccatc cattcgcgta gcgcagaaat aataatttca cgggcgatca tcgttgccgc
     7561 cggtaatgtc acccaccagc tgtgataatg ctcggttacc agcaccatgg cgatagccac
     7621 gagaacttta tctgccacag ggtcaaggaa agcaccaaac cgggtactct ggttccagcg
     7681 gcgtgccaga aaaccatcga accagtcagt caccgccgcg acgcagaaaa tgagcgcggc
     7741 ggcaaacggc gaccaggtga caggcagata aaagaccaat acaaagaatg ggataaggat
     7801 gacacggaac agtgtaagca acgtagggat attaaattgc ataatgacgg gtaactatct
     7861 gttgtcagta agattacccc tatgttgcta cagagacatc aatgtttcaa cgaccagaag
     7921 atcttttctg ccagaccttg cgaaataccc ggcacttttg caatttcctc gacgctggcg
     7981 ttacgtaaac cttgcaaacc gcccatatat ttcaacaaca tttgccgacg ttttggcccg
     8041 acgccttcaa tggtttccag ggaactggta tttttgacct tcgcccgttt tttacggtgc
     8101 ccgccaatcg cgtgatcatg tgattcatcg cgaatatgct ggataacatg cagcgcgggt
     8161 gaatctggcg gcaaactaaa tccctcacct tccggctcaa agaacagcgt ttccagtcca
     8221 gccttacgat ctgctccttt ggcaacgcca agtagcagcg gatgattttt atcccatgag
     8281 acatccagtt cggcgaagac atttttcgcc tgcgcaagct ggcctttgcc gccgtcgata
     8341 aggatcacat ccgggatctt actgtcgtca atggctttac cataacgccg acgcagcacc
     8401 tgattcatcg ccgcataatc atcgcccggc gtgatgcctg taatgttata gcgccgatac
     8461 tccgcacgca gcgggccgtt agcatcaaac accacacagg aagcgacggt ttgttcgccc
     8521 atggtatggc tgatgtcaaa gcactccatc cgcttcactt ccggcaattt caacacgctg
     8581 gcaagcgcgg tcagtcgctg gtgaacggta gattgctgcg aaagtttgct ggttaaggcc
     8641 gtcgccgcat tggtgcgcgc gagtttcaga taacgcgccc tatcgccgcg aggtttggtt
     8701 tgaacattaa tcttgcgtcc cgccagttct gaaagggaat cggcgagcag cgttttatcg
     8761 ctaagattaa aatcgagcag gatctcaccc ggtaaggtgc gcatctggct gccttgtaaa
     8821 tagaactgac ctacgaaggt ttctaccacc tcgctcagtt ccgtaccgcc aggcactttc
     8881 gggaaatagc tgcggctgcc gagcactttg ccctgacgaa tgaacaatac gtggacacaa
     8941 gccatgcccg catcgaacgc cacaccaata acgtcaaggt cgtcgccggt attggaaaca
     9001 aactgttttt cggtgacgcg tcgcaccgcc tgaatttggt cgcgaataca tgccgcttct
     9061 tcaaactcca gattctggct ggcagtttcc atacggctaa tgagttgcgt aagcacctga
     9121 tcatctttgc cagacaaaaa caggcgcaca tactcgacct gctgagcgta ttcttcttca
     9181 ctcaccagtc cttcaacgca cggtcccaga cagcgcccta tctggtattg cagacacgga
     9241 cgcgagcgat tgcgataaac actattttcg cactggcgaa tggggaaaat cttttgcagt
     9301 agcgccagtg tttcacgtac ggcatagcca ttcgggaacg ggccgaaata ttcacctttg
     9361 gcatgctttg caccacgatg catcgccaga cgcgggtggg tatcgccact caggaagata
     9421 aaaggataag atttatcatc gcgtagcaaa acgttgtaac gcggctgata gagtttgatg
     9481 tagttgtgtt ccagcaacag cgcttcggtt tctgtatgcg taaccgttac atcaatttgc
     9541 tggatctggg cgaccagcgc ttcggttttg cgcgaagcga ggttgctgcg gaaatagctg
     9601 gaaagccgtt ttttcaggtc tttcgctttg ccgacataga taaccgtacc accagcatcg
     9661 tacatgcgat aaacgcctgg ctggctggtt acggttttta aaaacgcttt tgcgtcaaac
     9721 tgatcactca ctgacttgat aatgtctccg cattacacag accatggcga attgccaggt
     9781 gagtcagctc aacatcgcca tgaatgttta gtttactgaa catacgatag cggtagctgt
     9841 tcaccgtttt cggactgaga ttgagctgtt ctgagatctc attgaccttc tggcccttag
     9901 tgatcatcag cataatctgc aattcacgtt cagacaaact ggcaaatggg ctttctgttt
     9961 tttctggttc gatctggctt aacgccattt gttgagcgat atcagaagca atgtaacgct
    10021 gccctgaata gacagaacga atcgcactca cgacttcctg cggagccgcg cctttgctga
    10081 ggtagccagc agcacctgcc tgcatgactt tcgctggtaa agggttttct gtatggaccg
    10141 taagcatgat gattttaaca tcagctgtgg aacgcgcgat tttacgcgtc gcctcaagac
    10201 cgccaatgcc cggcatactc atgtccatta gcaccacgtc aacggcattt gtccggcacc
    10261 acttaacggc gtcttcaccg cacgatgcct ctccgacgac ttttataccc tttatatctt
    10321 ccagaatgcg tcgtatccct gcgcgcacca gttcgtggtc atcaacaagt agaacgttga
    10381 tcaaaggaat atctccagaa atagggataa cgctactgat agttagtcat tcgtatatta
    10441 gcggttttta ttgcaacttt gaaacgttaa aaatggtggg tattcgattt ttctctcgtt
    10501 tttgtcaatt cgattgtcca cagttatggt cacgcccgtc accaacggct taccggatcg
    10561 tttcacagta agccctacga tgaaatgtga caaaaattga ctttattcag caaaaatgaa
    10621 aatcagcccg ctgaatatgt aacattaatt aacccgaaca atacgggcaa aaaacattca
    10681 tgataaaaat atttattggt cattatatta acgtatttta tagcactgcc gatatcacgc
    10741 tcaaaaaaca accactgcta tttttagcaa agcttatggt atactccgcc gccttaacat
    10801 ttttcaccgc aaattttcat tgcaacatga cgaggaaaat aaatgagtac gcctgatttt
    10861 tctactgccg agaataatca agaactggca aatgaagtct cctgcctgaa agcgatgctg
    10921 acgctgatgc tgcaggcgat gggacaagct gacgcgggcc gcgtgatgtt aaagatggaa
    10981 aaacagcttg cgctgatcga agacgaaacc caggctgcag tattttccaa aacggttaag
    11041 caaattaaac aagcctaccg tcagtaatat aaaaccggct gagagcgtgc ctttcagccg
    11101 gttattgaca aaatcatcgc tatctctgct gatgtcttcc cttccgccgt ccatttcaga
    11161 atttcttttt cgcgcttgct gaaattcatc tctggcgtca tcactatttc atcatttaaa
    11221 cgcatcagag ccatcagact ttcgcgcacc agtaactgca tttttaattg cagttcatca
    11281 ctaagaatgg gtatttcgcg cgtgctgcaa cgggaaaagg acaaaaagcc cagcgcccga
    11341 tttggcagca ttaaatactg agtgacaccg cggcgtaaac catgtgcgtg cgcggcttcc
    11401 cataatggct gtgcttcgct gaataagtca tcattccaca ttaaatggtc ctgactaaag
    11461 ttttcagggt tcagcacagg atcaattgcg agaaaatttt ttgcctgata ataactaacc
    11521 cacgactcag ggtaattggt gtagaaagcc actttaggtc gcgtgaatag taccgggtgg
    11581 cgaacacata acgaatagta atcgtactcc agctgctgag cctgaagctc tatttcatgg
    11641 tagacctctt ctgcggcctc catcctctga aaacgcaaca gcatcgtgcg acgccagctg
    11701 aaaaaatccg tatcctgcat agtaaaccgc aacgcccctg agagtgagta tcatttataa
    11761 tgataattca aactatcaca taaataagat ttatatataa tttatattat tcaggcaatg
    11821 aattactttt gcaagccatc gcattctctt atgttattaa tgagttatgc tgatttgtta
    11881 agcagtttta tcacgcttga aatggcgtcc cgccccgaca ggtgaatcgt cggggctgat
    11941 tttttcttat tattgcagca gaagcttctc gaggaactgg cgggtgcgag gctgctgggg
    12001 atcggcaaat aacgcttttg ctgccccctg ctcgactatc cgcccctggt ccataaagat
    12061 cgcccggtcc gcaacatccc gggcaaagct catttcgtgt gtcacaatca ccatcgtgcg
    12121 cttttcctgc gccagctggc ggatggtgtt caggacttcg cccaccagct ctggatccag
    12181 cgccgacgtt ggctcgtcaa acaaaatcac ctcaggacgc attgccagtg cacgcgcaat
    12241 cgcaacacgc tgctgttgac cgccagacaa acgacgtgga tagctggttt ctttaccggc
    12301 cagcccaact ttcgccagca gctcgcgagc gcgcgccgtg gcctcttctt tcggttcgcc
    12361 tttgacgatc accggccctt caataatgtt ttccagcacc gtacgatgcg gaaacaaatt
    12421 aaagttctgg aagacaaacc cgacgtgctg acgcaactgg cgaatcagcg atttttgctg
    12481 acttaatgaa cgtgcagtat caatagtgat atcgccaacg gtgatcgttc ccgcttccgg
    12541 ttgttccagc agatttatgc tacgtagcaa tgtggttttg ccggaaccac tcggaccgat
    12601 aattgccacc acttcgccag gctttacctc gaggtcgata ccgtgcagca ccgtctgacc
    12661 gtggaatttt ttcaccaggt tcttaacttc aatggcactc attttggttc tctctcctgg
    12721 cgattaagtt gattctcaaa atggttctgc aacgtcgata acacagtcgc catgatccag
    12781 tagatgagcg aagccgccag gtacatggtg aacacctcca gcgtgcgcga ggtaatcaac
    12841 tgcgcctgac ggaacagctc cggcacctgg attgtcgcgg ccagcgacgt atcttttacc
    12901 agactgataa agctgttcga cagcggcggc aacgccaccc gcgccgcctg tggcaaaatc
    12961 gcgcgacgca tggtctgcca cggcgtcata ccaatactgg cagcggcttc ccactgacct
    13021 ttatcaatag aagaaatggc ggcgcgcagc gtttcggcgg cataggcggc agtgttcagc
    13081 gacagaccaa tcattgctga cggaatggga tctaattcga taccaaactg cggcaggccg
    13141 taatagatca taaacagttg tgcgatgagc ggcgtaccac gaaaaataga gatataaaaa
    13201 cgcgccagcc agcgcacggg ccagattggc gacaggcgca tcagcgcgag aataaacccg
    13261 agtagtaagc caaaaaacat gccgccgata ctgagttgca gggtataccc agcccctttc
    13321 aacaggaacg gcaaggaatc aataaccagt tgtatacttt cttgcatgag cgttcccgga
    13381 gttaaacgtg gggatgatag gcgaacagcg caggcgcgcc gccggtatga ataaacagaa
    13441 tcggcccttc atctttgaag cgtttctgac tgataccgtc aatcagcccc gccatcgctt
    13501 ttccggtata cacaggatca agcagaatac cttccagacg cgccagcaat ttcactgctt
    13561 ccatgccttc gtcgttcggc acgccgtagc caggtgcaaa atagtcatcc cagagtaaaa
    13621 tttccgctga tgcggtcagc tccagttctt tcgcaatcgc ctgttgtagg ttaaccactt
    13681 tcggcaattg atcggcaacg gaacgcgaca cggtcacgcc aatcagttcg ctttcaggca
    13741 tcaggtgttc cagcccaaca gccagtccgg cgtgagttcc ggcactgccc gatgcgacta
    13801 ccaccgacga aatattaacc gccccttcac actgttgcgc gatttccagc gcactctcca
    13861 cataacctag cgcgccaaga gcattagaac cgccaaccgg aatgacatac gggcgaaagc
    13921 cttgtgcttc gactcgcgtc gccagctctt ccagttgggc attgggatcg gtcagtgcgt
    13981 cgcacatttc aatctgggta ttgaacagat ccagcaacaa acgattgccg ttggttaaat
    14041 agttttctgc ggttgtgcca ataggatttt ccagcagcgc cacgcagtgc agaccgagtt
    14101 tcgccgcgac tgcggcagtc tggcgcacat ggttagactg gatcgcgccg gcagtaatca
    14161 gcgtatcggc accttcgcgc agggcatctg ccgcgagaaa ttccagctta cgtaatttat
    14221 tgccgcccat tgccatgggg gtgacctcat cccgtttgat gaaaatttcc cgtccaagat
    14281 aatcagaaaa gcgcggcaga tattcgagcg gcgttggcgc gccgataaac tccaaccgtg
    14341 gaaaacgggt taaattatgc agtggcataa cagcctccga tgcgtgttgt tgtgattttc
    14401 ttattatgca cgctgaaaac gcgtaaataa aaaaggcgct agtgaaagcg cccttttttg
    14461 tcattatgct gattatttgg tcacatcagc accaaaccat ttttcggaaa gagcttgcag
    14521 agtgccgtct ttttgcattt ccgcaattgc atcattcacc gctttcagca ggtcttcatt
    14581 tcctttacgc agcgccacgc cagactcctg acgggagaat gcttcaccgg ttactgccag
    14641 cgtatcgttg gttttcttca ccagatccag cgccgccaga cgatcaacga ggatcgcatc
    14701 gatacgccct acgcgcagat cctgatattt ggtcgggtca tcatcatagg tacgcacatc
    14761 gacaccctga acattctgcc gcagccactc ttcatagttg gtgcccagac cgacacccac
    14821 ttttttgcct ttcagatcag cagccgtttt aatggtgcct tcgttacctt ttttcaccag
    14881 cgcctgaata ccggaaatgg tgtaaggggt agagaaatcg tattttttct tgcgctcgtc
    14941 agaaatggtg acctgattaa tcaccacatc aatgcgttta gagtccagcg acgccagcat
    15001 accgtcccat ttggtcggtt ttagtgacgc ctcaacgcca agatgttttg ccagctgttg
    15061 agcaaattcc acttcaaaac cggttaattt gccgtcatcc ccctgaaaac tgaacggcgg
    15121 ataagtgcct tccagcccta ccagcagcgt gccgcgctct ttgactttat taagcagacc
    15181 ttcatctgca aaacttttaa cgctcatgcc cgcaaccagc gccacgccca tcacacccat
    15241 caatgcctga cgtcccagat gtgctaattt catattcacc ccgaatgttg ttatgtctgt
    15301 ttgcagtgta gagccattgt ttgtaaacac aaaaacaact ccgctacatc ttattcttat
    15361 ttaatatata tcagaagcag gcaggctgga ggatttctgc acaagtccgg tacaggtttg
    15421 gcgctgatag tgttgatact tccgtaatgc gatgcggtaa ttgttggtgc tggtggtggg
    15481 cagccacggg gcgaggattt catcaaggaa accgtcctgc agctgatcga gggaaatatt
    15541 ttgctcatgt agatgattac ccaaacggcg cagacgaacg acatattcac gcaccgtacc
    15601 tgggctcatt tcagtttgct caaaaagaaa tttcttaaag ccgataatat cgaaaaaatc
    15661 actctgcgtt ttgcaatgta aatcaccgca aaatcggcac aatgccgccc atgattgttg
    15721 ttccgtttgc cagccttttt catcaagcag cgtgtccagg cgggaaatct cgattttatt
    15781 cactattttg ccgtcgcgaa ctaaggtaat gcgatcgagt aatttacggc aatgcgcgca
    15841 atgggtctgg ctgtgtttaa agtctttaag atagcggctt aatggccgtc ttttcaggtg
    15901 ctgcaccatc attaagaact cctggtagtc aaagttaaag tgcggcattt actgacgtta
    15961 taacttaccc agtttagtgc gtaaccgttt aatggcctgg ctgtgtaact gactgacccg
    16021 cgattccccg acctccagca ccgcgccaat ctctttgaga ttcagctctt cctggtaata
    16081 gagggttaat accagttttt cgcgctccgg caacgtttcg atggcttcca tcacccgctg
    16141 gcgcagatta ctgtccagca gttgttgtag cgggttttct cgctgatgat catcagtaac
    16201 cagttcgatg ctatcgccgt gctcttcacg ccactcatcg taggagaaga gctggctgtt
    16261 attggtgtcg agcagcattt gacgataatc ggcaatatcg atccctaaac gttccgctac
    16321 ctcagtttcc gtggcgttgc ggccaagttc ctgctccagt tgccctattg cctgtgccac
    16381 ttcacgcgcg ttgcgtcgca cgctgcgcgg cacccagtca cggctgcgaa gttcatccag
    16441 catagcgcca cggatacgct gcactgcgta agttgtaaat gccgttcctt gtagggcgtc
    16501 atagcgttcg acagcattaa gtaacccaat gccgcccgcc tgtagcagat cgtcaagttc
    16561 cacgctcgcg ggcagtcgaa cctgcaggcg caatgcttcg tgacacacca gcgggacata
    16621 acgctgccac agcgagtgtt tatccattac accttcagcg gtatagagtg aattcacgat
    16681 aaacagccct gcgttatatg agttatcggc atgattatcc gtttctgcag ggtttttaat
    16741 cggacgatta gtgggtgaaa tgaggggtta tttgggggtt acgcaaaaag ccgaatgtaa
    16801 aaaaccccgt cggtggcggg gtttgagagg cattcagacg attaaccctg cagcagagac
    16861 agaacctgct gcggtacctg gttggctttt gccagcacgg agttaccggc ctgcaggata
    16921 atctgcgctt tcgacatgtt ggacacttcg gtcgcatagt cggcgtcctg aatacgggac
    16981 tgcgcttcag acaggttggt agtggtgttg ttcaggttgg tgactgcgga atccaaacgg
    17041 ttttgcaccg cacccaggga ggagcggaat ttgtcgatgg agctgatggc ttcatccaga
    17101 gctttcaggg gatcggctgt cgtagctgct tttgtttcag catctgtcgt caatttacca
    17161 tcagcatctt tataaactgc cttaccgctg ccgttagtca ctgaaccatc atcctgtagg
    17221 taaaaatcta cagtatcaga tgtggtcaac gcctcgttgt tatcaacata aaccgaatca
    17281 acaccttttg ttacagcgcc agttgtgccg ttgacgctat aacctacagt agtgaaatca
    17341 acattgtttc ccgaggttga tacagcttta tcgaaagctg cctgatcaac ttttgcacct
    17401 gttactgaat aagtaatagt gtccggcgta gtactattac cggtaaagga gatagaggta
    17461 ccgccaatgt caatggttgt cgctttggta ttattggctg caagtgtgga cagactagcc
    17521 tcagtcaaag aagcaccaga gccgtttttc gttaagcgcc cagttgtatc aatgtagagt
    17581 ttatctccat tgcttgccgt aattttacca tcactgctga tctgaacatc ctgtgcagaa
    17641 ccatcgatag taatagtgcc cttagcggta tcaccaactc ccggggtcaa atatttctga
    17701 acatcggcag ctgaagccgt tgtggtatca aaagagtatg acttacttgc actgtcatac
    17761 ttgtaattcg ttgcactagc cgccgttccg aagccgttat taatggttgc ctgaacagta
    17821 tcaccatcac taactccagc cagcaaatca gacgctttag cagcatcgta acccgcactc
    17881 acagtatatt tgttgccgac cactgttgca ttagcagcta ccaagtcagc tttagatgca
    17941 gcagtattag ccacagcccc gccaccattc acattaaacc cattcagccc cagcgtatca
    18001 gagtcaattt tcttcagatc aatagtgata gtctggccgt cattcgcacc aacctgaatt
    18061 ttcatcgaac cgtcttttgc cagtacgttc acgccgttga actgggtctg accggatacg
    18121 cggtcaattt cgtcgagacg ggatttgatt tcgtcctgaa tggagtccag atccgaatca
    18181 gagttagtcc cggtagaagc ctgaaccgtc agctcacgga tacgctgtaa gttgttgttg
    18241 atttcggaca gcgcgccttc agtggtctgt gcaacagaaa taccgtcgtt ggcgttacgt
    18301 gcagcctgag tcaggccttt aatgttagaa gtaaaacggt tagcaatcgc ctgacccgcg
    18361 gcgtcatcct tcgcgctgtt aatacgcaag ccagaagaca gacgctcgat agaactcgac
    18421 agcgcagact ggttcttgtt gatattattt tgagtgatca gcgagaggct gttggtatta
    18481 atgacttgtg ccatgattcg ttatcctata ttgcaagtcg ttgattacgt tttgggtttc
    18541 cacccgtcgg ctcaatcgcc gtcaaccctg ttatcgtctg tcgtaaaaca acctttagaa
    18601 tttttttcac aaacagccat tttttgttag tcgacgaaat actcttttct ctgcccctta
    18661 ttcccgctat taaaaaaaac aattaaacgt aaactttgcg caattcaggc cgataacccc
    18721 ggtattcgtt ttacgtgtcg aaagataaaa ggaaatcgca tggcaagtat ttcatcgctg
    18781 ggagtcgggt caggtctgga tttaagttca atccttgata gcctcaccgc cgcgcaaaaa
    18841 gcgacgctaa cccctatttc aaatcagcaa tcgtcgttta ccgctaaact tagcgcctac
    18901 ggtacgctga aaagcgcgct gacgactttc cagaccgcca atactgcatt gtctaaagcc
    18961 gatctttttt ccgccaccag caccaccagc agcaccaccg cgttcagtgc caccaccgcg
    19021 ggtaacgcca tcgccgggaa atacaccatc agcgtcaccc atctggcgca ggcgcaaacc
    19081 ctgaccacgc gcaccaccag agatgatacg aaaacggcga tcgccaccag cgacagcaaa
    19141 ctcaccattc aacaaggcgg cgacaaagat ccgattacca ttgatatcag cgcggctaac
    19201 tcatcgttaa gcgggatccg tgatgccatc aacaacgcaa aagcgggcgt aagtgcgagc
    19261 atcattaacg tgggtaacgg tgaatatcgt ctgtcagtca catcaaatga caccggcctt
    19321 gataatgcga tgacactctc ggtcagcggt gatgatgcgc tacaaagttt tatgggctat
    19381 gacgccagtg ccagcagcaa cggtatggag gtctcggtcg ccgcccagaa tgcgcagctg
    19441 acggtcaaca acgtcgccat cgagaacagc agcaacacta tcagcgacgc gctggaaaac
    19501 atcaccctga acctgaacga tgtcaccacg ggcaaccaga cgctaaccat cactcaggac
    19561 acctccaaag cgcaaacggc gattaaagac tgggtgaatg cctataactc gctaatagat
    19621 accttcagca gcctgaccaa atacaccgcc gtagatgcgg gagctgatag ccagagttct
    19681 agcaatggcg cactgctcgg cgactccacg ctgcggacga ttcagacgca gttgaagtcg
    19741 atgctgagta ataccgtcag ttcttccagc tataaaacgc tggcgcagat tggtatcacg
    19801 accgatccca gcgatggcaa actggaactg gatgccgaca aactcaccgc tgcactgaaa
    19861 aaagatgcca gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac cggcatcaca
    19921 accaccatcg gcagcaacct gaccagttgg ctttcgacaa cgggcattat taaagccgct
    19981 accgatggcg ttagtaaaac cctgaataag ttaactaaag actacaacgc cgccagcgat
    20041 cgcattgatg cgcaggtcgc gcgctacaaa gaacaattta cccaactgga cgttttaatg
    20101 acctcgttaa acagcaccag cagctactta acgcagcagt tcgaaaacaa cagtaattcc
    20161 aagtaatcat tattcatcgg gagacaggtc atgtacgcgg caaaaggcac ccaggcctat
    20221 gcgcaaattg gcgtcgaaag caccgtaatg agcgccagcc aacagcagtt ggtcaccatg
    20281 ctatttgatg gagtgctgag cgcactggtt agagcgcgac tgtttatgca ggacaacaat
    20341 cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa cggactgcgg
    20401 gtgagtcttg atgaagagag caaagacgaa ttaacccaaa acttaattgc tctttatagc
    20461 tatatggtca ggcgtttgct gcaagccaat ttacgcaacg atgtctccgc agtcgaagag
    20521 gtggaagcat taatgcgtaa tattgccgat gcctggaaag agtctttact ctccccttct
    20581 tcgattcagg acccagtctg atgaacaatg caccgcattt gtatttcgcc tggcaacaac
    20641 tcgtcgaaaa aagccagctc atgttacgcc tggcaacgga agaacaatgg gacgaactca
    20701 tcgctagcga aatggcgtat gtgaatgcgg tgcaggagat cgcacacctg actgaagagg
    20761 ttgccccgtc cactacgatg caggagcagc tccgcccgat gctgcacctg attctcgaca
    20821 acgaaagcaa ggtaaagcag ttattacaga ttcggatgga tgaactggcg aaactggtcg
    20881 gtcagtcatc ggtgcaaaaa tcggtgttaa gtgcctatgg cgatcagggc ggctttgtgc
    20941 tggctccgca ggataacctc ttttgaatct taatgagccg atggctcgcg aataatccga
    21001 ttacggctac gcttctaata ttccccttga atggagtcga agaatgcgta atcccacgct
    21061 gttacaatgt tttcactggt attacccgga aggcggtaag ctctggcctg aactggccga
    21121 gcgcgcagac ggttttaatg atattggtat caatatggtc tggttgccgc ccgcctataa
    21181 aggcgcatcg ggcggctatt cggtcggcta cgactcctat gatttatttg atctaggtga
    21241 gtttgatcag aaaggcagca tccctactaa atatggtgat aaagcacaac tgctggccgc
    21301 cattgacgcc ctgaaacgta atgacattgc ggtgctgctg gacgtggtag tcaaccacaa
    21361 aatgggcgcg gatgaaaaag aagctattcg cgtgcagcgt gtaaatgctg atgaccgtac
    21421 gcaaattgac gaagaaatca ttgagtgtga aggctggacg cgttacacct tccccgctcg
    21481 tgccgggcaa tactcgcagt ttatctggga tttcaaatgt tttagcggta tcgaccatat
    21541 cgaacaccct gatgaagatt gcatttttaa aattgttaac gactataccg gagaaggctg
    21601 gaacgatcag gttgatgatg aactcggtaa tttcgattat ctgatgggtg agaatatcga
    21661 ttttcgcaat catgccgtga cggaagagat taaatactgg gcgcgctggg tgatggaaca
    21721 aacgcaatgc gacggctttc gtcttgatgc ggtcaaacat attccggcat ggttttataa
    21781 agagtggatc gaacacgtac aggaagttgc gccaaagccg ctgtttattg tggctgaata
    21841 ctggtcgcat gaagtcgata aactgcaaac gtatattgat caggtggaag gcaaaaccat
    21901 gctgtttgat gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg gtcgcgacta
    21961 cgacatgacg cagatcttca ctgggacgct ggtggaagcc gatccttttc acgccgtaac
    22021 cctcgttgcc aaccacgaca cccaaccgtt gcaagccctc gaagcaccgg tcgaaccgtg
    22081 gtttaaaccg ctggcgtatg ccttaatttt gttgcgggaa aatggcgttc cttcggtgtt
    22141 ctatccggac ctctacggtg cacattacga agatgtcggt ggtgacgggc aaacctatcc
    22201 gatagatatg ccaataatcg aacagcttga tgagttaatt ctcgcccgtc agcgtttcgc
    22261 ccacggtgta cagacattat ttttcgacca tccgaactgc attgccttta gccgcagtgg
    22321 caccgacgaa tatcccggct gcgtggtggt catgtcgaac ggagatgatg gcgaaaaaac
    22381 cattcatctg ggagagaatt acggcaataa aacctggcgt gattttctgg ggaaccggca
    22441 agagagcgta gtgacagacg aaaacggcga agcgaccttc ttttgcaacg gcggcagcgt
    22501 cagcgtgtgg gttatcgaag aggtgattta aatccatccc cggcggcaag ccggggagat
    22561 ttcattacgg cagtggcgtc ggtaacggcg ctttatccag cgcggcggca cactctgccg
    22621 tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatggtgtta ccgtcacgct
    22681 caacctcgta cagctcacgt tttacggtaa cgttagtgag atcatcagac atcaatgtca
    22741 acttacccgg cacggcaatc ccccgctgcc actgacggca atccaaggta tcgccctctt
    22801 tagtgacaat caggctgcct atcgcttccg gactgaccag cgcacgttgc ggccctttgg
    22861 tctgccagta gcccgccagc cagtccggcg cgggggtttt cacgacattg ttatagtttt
    22921 cgacttcggc acagcccgcc agcaacatga gcgcgcctgc aattgcgagt tttttcatca
    22981 ttcgtcctgc atgagaagaa aagaggattg tggcattaaa gctctgatgt cgccagcctt
    23041 tccaggttca gagagaaatt tgatctaact attatttttg tgaattatta ccccgaaaat
    23101 aaatttgcat ggtgtatgat tcgcggctct tgtttcaaac cttctgagtt cagaggctac
    23161 attcatgtca tggcagcaat tcaaacacgc ctggttgatt aaattctggg cacccatccc
    23221 tgcggtcatc gcggcgggta ttctctccac ttactatttt ggcattactg gcaccttttg
    23281 ggctgtcacg ggtgaattta cccgttgggg cggtcagctc ctgcagctgt ttggcgtcca
    23341 tgctgaagag tggggttatt ttaaaattat ccatctggaa ggatcgccat taacccgcat
    23401 cgacggcatg atgatcctcg gtatgtttgg cggctgcttt gctgctgcgc tgtgggccaa
    23461 caatgtcaaa ctacgcatgc cacgcagccg tatccgcatt atgcaggcca tcattggcgg
    23521 cattatcgcc ggttttggcg cgcgtctggc aatgggctgt aacctggcgg cgttctttac
    23581 cggcattcct cagttctcgc tgcatgcctg gttctttgcc atcgccactg ccattggttc
    23641 atggtttggc gcgcgcttta ccctgctgcc catcttccgt attcccgtga aaatgcagaa
    23701 agtttctgcc gcctcaccgc tgacgcaaaa accggatcag gcgcggcgtc gttttcgtct
    23761 cgggatgctg gtctttttcg gcatgctggg ctgggcgctg ctcacggcga tgaaccaacc
    23821 aaaactgggg ctggcaatgc tgtttggcgt cggctttggt ttactgattg aacgtgcgca
    23881 aatctgcttt acttcggcgt tccgcgatat gtggatcacc ggacgtaccc atatggcgaa
    23941 agcaatcatt atcggtatgg cggtgagtgc catcgggatc ttcagttacg tacagttagg
    24001 cgttgaacct aaaatcatgt gggcgggacc aaatgcggta attggtggtt tactttttgg
    24061 ttttggcatc gtgctggcag gcggctgcga aaccggctgg atgtaccgcg cggtagaagg
    24121 ccaggtgcac tactggtggg tcggtctggg caacgtgatc ggctcaacga ttctggcgta
    24181 ttactgggat gatttcgctc cggcgctggc caccgactgg gacaaaatca acctgctgaa
    24241 aacttttggt ccgatgggag gcctgctggt gacatatttg ctgctgtttg ctgcgctgat
    24301 gttgattatc ggctgggaaa aacgcttctt ccgccgtgcg gcaccgcaga ctgctaagga
    24361 gatcgcatga aaaatatcgt tcctgattac cgccttgata tggtgggtga accctgccct
    24421 tatccggcag tcgcaaccct tgaggcgatg ccgcaattaa aaaaaggtga gatcctagaa
    24481 gtggtgagcg actgtccgca gtcgatcaat aatattccac tggatgcgcg taatcacggc
    24541 tacactgtgc tggatattca gcaagacggg ccgaccattc gttatttaat tcagaagtaa
    24601 tccctcattc ccgctgtggc ccacagcggg aacatcgtta cctctgacac atagatttta
    24661 cccgccccct ctcctattct tttcagtatg atctcaggag gttgctatgt gtggacgctg
    24721 tgcccaatcc caaacgcgtg aggattacct tgcgcttctc gcggaagata ttgaacgcga
    24781 tattccctac gatcccgaac ccattggcag atacaatgtc gcgccgggaa ccaaagttct
    24841 tctgctcagt gaacgtgatg aacaccttca tctggatccg gttttctggg gatatgctcc
    24901 cggatggtgg gataaaccgc cgctgattaa cgcccgcgta gaaactgcct caaccagtcg
    24961 catgtttaaa ccgctctggc aacatggtcg ggcaatctgt tttgccgatg gctggtttga
    25021 gtggaaaaaa gaaggcgaca aaaaaagcct tattttatct atcgcgctga cggacaacct
    25081 gtttttatgg ccgcgatagg tagcataccg tttgagcgtg gtgacgaagc cgaaggattt
    25141 ttgatcgtca ctgcggcggc agatcaaggt ctggtagata ttcatgaccg ccgcccgctg
    25201 gttctgtcgc cagaagctgc acgggaatgg atgcggcaag acattggtgg taaagaagcc
    25261 tcagaaatcg ctaccagaag ctgtgtgcca gcaaaccagt tcacctggca ccctgtgtcg
    25321 cgcacggtgg gtaatgtcaa aaaccagggc gcggagttaa ttcaacctgt ttgttgacat
    25381 cctccacgcc ctgaatgacg aggccccctg ctacgttcag gctgtcgcct gaatcatttc
    25441 ggtgggttcc tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc
    25501 gtgccccgcc gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca
    25561 tacctggcat ctgaatttac tttgtgacag gcgattttct ttcgctgtat gaccacagca
    25621 cgcgcaacgc tggcttgtgt acgctggccc gccac
//
LOCUS       DAJTYF010000046        25343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTYF010000046 DAJTYF010000000
VERSION     DAJTYF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.046"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            6..524
                     /locus_tag="OO590_002057"
     CDS             6..524
                     /locus_tag="OO590_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6637425.1"
                     /translation="MVAAITCISQPKKTTLRDKAMVNYAFDYLRSPGSLPLTTAATEL
                     SAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGL
                     GVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYK
                     NAERLLAGLPAH"
     gene            complement(534..1961)
                     /locus_tag="OO590_002058"
     CDS             complement(534..1961)
                     /locus_tag="OO590_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6637426.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1958..2665)
                     /locus_tag="OO590_002059"
     CDS             complement(1958..2665)
                     /locus_tag="OO590_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6637427.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2823..3806
                     /locus_tag="OO590_002060"
     CDS             2823..3806
                     /locus_tag="OO590_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6637428.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3876..4358)
                     /locus_tag="OO590_002061"
     CDS             complement(3876..4358)
                     /locus_tag="OO590_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6637429.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4593..7175
                     /gene="leuS"
                     /locus_tag="OO590_002062"
     CDS             4593..7175
                     /gene="leuS"
                     /locus_tag="OO590_002062"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6637430.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7190..7771
                     /gene="lptE"
                     /locus_tag="OO590_002063"
     CDS             7190..7771
                     /gene="lptE"
                     /locus_tag="OO590_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6637431.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7771..8802
                     /gene="holA"
                     /locus_tag="OO590_002064"
     CDS             7771..8802
                     /gene="holA"
                     /locus_tag="OO590_002064"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6637432.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRTLFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8804..9445
                     /gene="nadD"
                     /locus_tag="OO590_002065"
     CDS             8804..9445
                     /gene="nadD"
                     /locus_tag="OO590_002065"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6637433.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9469..10080
                     /locus_tag="OO590_002066"
     CDS             9469..10080
                     /locus_tag="OO590_002066"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6637434.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10340..10657
                     /gene="rsfS"
                     /locus_tag="OO590_002067"
                     /gene_synonym="rsfA"
     CDS             10340..10657
                     /gene="rsfS"
                     /locus_tag="OO590_002067"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6637435.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10661..11128
                     /gene="rlmH"
                     /locus_tag="OO590_002068"
     CDS             10661..11128
                     /gene="rlmH"
                     /locus_tag="OO590_002068"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6637436.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11159..13060
                     /gene="mrdA"
                     /locus_tag="OO590_002069"
     CDS             11159..13060
                     /gene="mrdA"
                     /locus_tag="OO590_002069"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6637437.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13063..14175
                     /gene="mrdB"
                     /locus_tag="OO590_002070"
                     /gene_synonym="rodA"
     CDS             13063..14175
                     /gene="mrdB"
                     /locus_tag="OO590_002070"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6637438.1"
                     /translation="MTANPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14186..15274
                     /gene="rlpA"
                     /locus_tag="OO590_002071"
     CDS             14186..15274
                     /gene="rlpA"
                     /locus_tag="OO590_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6637439.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15413..16624
                     /gene="dacA"
                     /locus_tag="OO590_002072"
     CDS             15413..16624
                     /gene="dacA"
                     /locus_tag="OO590_002072"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6637440.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16735..16998
                     /locus_tag="OO590_002073"
     CDS             16735..16998
                     /locus_tag="OO590_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6637441.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17099..17740
                     /gene="lipB"
                     /locus_tag="OO590_002074"
     CDS             17099..17740
                     /gene="lipB"
                     /locus_tag="OO590_002074"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6637442.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17999..18952
                     /locus_tag="OO590_002075"
     CDS             17999..18952
                     /locus_tag="OO590_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6637443.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19161..20126
                     /gene="lipA"
                     /locus_tag="OO590_002076"
     CDS             19161..20126
                     /gene="lipA"
                     /locus_tag="OO590_002076"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6637444.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20227..20430)
                     /gene="tatE"
                     /locus_tag="OO590_002077"
     CDS             complement(20227..20430)
                     /gene="tatE"
                     /locus_tag="OO590_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6637445.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20559..21347)
                     /locus_tag="OO590_002078"
     CDS             complement(20559..21347)
                     /locus_tag="OO590_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6637446.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21440..21823
                     /gene="crcB"
                     /locus_tag="OO590_002079"
     CDS             21440..21823
                     /gene="crcB"
                     /locus_tag="OO590_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6637447.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21877..22086)
                     /gene="cspE"
                     /locus_tag="OO590_002080"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21877..22086)
                     /gene="cspE"
                     /locus_tag="OO590_002080"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6637448.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22261..22821)
                     /gene="pagP"
                     /locus_tag="OO590_002081"
     CDS             complement(22261..22821)
                     /gene="pagP"
                     /locus_tag="OO590_002081"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6637449.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23410..24795
                     /gene="dcuC"
                     /locus_tag="OO590_002082"
     CDS             23410..24795
                     /gene="dcuC"
                     /locus_tag="OO590_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6637450.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24836..25177)
                     /locus_tag="OO590_002083"
                     /pseudo
     CDS             complement(24836..25177)
                     /locus_tag="OO590_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         6467 a         6296 c         6240 g         6340 t
ORIGIN      
        1 tttttatagt tgcagcaatt acctgtatta gccaacccaa gaaaacgacg ttgcgtgata
       61 aagccatggt gaattatgcc tttgattatt taagatcacc aggcagtcta ccgttaacca
      121 cggcagccac ggagctttcc gcgattcatg gtcactcaac atcgcaatat cgccttggcg
      181 aattttatct tcatggtagc gacggtaaac cactggatta tacacaggcg agatactggt
      241 atgagcaatc agcagaacag gaaaatccgc gcgcacaaag caaactgggg tggatctacc
      301 tgaaaggcct gggagtcaaa cccgataccc gtaaagcaat tctctggtat aaggaagcag
      361 ctgaacaagg gtatgctcat gctcaatata ctttgggttt gatctacaga aatggcactg
      421 gtattaatgt taaccattat gaatctcaaa aatggttaaa actggccgcc aaacaacatt
      481 acaaaaatgc ggaaagatta cttgccggac ttcccgcaca ttaacgtcga ctgtcacccg
      541 aagagatgga gtattttccc tatcaaaccg gcaaagataa aaatgtagaa aataattttg
      601 atgacgccca gacagcccag cccgccatcg tcaacgggca ttggctgcgg ttgcggctcc
      661 ggctcgacgg tttcctgacc ggtaaaattc gccgcctgaa ttaataatcg acgaataatt
      721 aatgcttcca gtggacaacg atcttctgca tcaaggcggc tcacaatcgg cattaatgat
      781 tcttctgtcg gttggctggc ctgcactaat tgtttggcaa ctaaatataa tggccataag
      841 ggatgttctg tatcacgcgt gagtgagatc cgcggcttac gtcctttgtg caaaaccatc
      901 tcgaccagtg aacgccccag cgcagagagt ttttcatcgc tgagtatttc gtataaatga
      961 aaacgcgtac gggcgctgtc gccggggaca taaagtagcg gttttccctg atcgttaacc
     1021 agatcacgcg cttcaatcat catcaccact aatgggtaat aatcgacccg atgctgacgg
     1081 caccattgca acattaaagc atccgcctgg gtggagggat actcgcgcca gtagtaacaa
     1141 agttctgcca gcaggctttc gccttctcca caatagacgc gttgtgcgta ttcataataa
     1201 cgcgcatctt cattatcagg ttcagcttcc cgccacgacc gggcgtaatc caggagttcg
     1261 gggattccca ggcggcttgc gctataccag cggagtaaat ttctgtgcag ttttgcatca
     1321 tcggggataa tcactgcgcg atgttgcgct aaatacaatg tcagccattc tggatgcgat
     1381 gaccaaatcc gagcgaacat ttggtaaaaa tcgatggtgt cattttgcac ggcaacgggc
     1441 agatgcagaa tggtctggaa gttaaaaaca ttccccttcg caatggcata taaaaaggat
     1501 tccagttctt cttcgtctat ttccccgacg ttttcctcct gcaaccaccg caatctttct
     1561 gctaataaca ccacgcactc gaaggataaa tgggcagtgt tcatggcgat tgtgcacagc
     1621 gaccagcgta attcatcaat ctcttccatc gagcaataat ttaattgctg aatgaatcgc
     1681 tgccaggcgg agggcagaaa acgttcgcta tcagaggcaa gtaacgcgcg aaaggcgagc
     1741 agaatttcat gttctgctac ctcatattct tctggttgcc aaacagattt agcaggcgac
     1801 tgcgcaatcc gtagcgcctc ctcatacgcc tgacgaagtt gtttaaaacc ctgcggatcg
     1861 gtttctggat gaaaggacgg taacagcgcc agataagcgc ggcggataat atcgacgtca
     1921 gtcgtttcct ctatatcgag gatcttccag caatttttca taagtcgctc atatccttaa
     1981 gcgtttccgg gcatgtgggg tagtcagcca gtggttgcca ggacaaggta gggtaatacg
     2041 catcatcact ggcaaataat tgataaaaca tatcattgcc ggaatcgtcg tattctttac
     2101 gcgctaagaa ttcgcgtaat aaaggcagat gatcatcttc gggagactgc caatattgcc
     2161 gaccaaaggt ataaccggca aagtattgcc gccagttact ataataacgt agagcccgta
     2221 gatgaatacg cgacaggatc catagacttt cttcttcact caaccagtta ttaagtacgc
     2281 ccattcgact taaaaaaccc atccggacat aatcccatgc tttaattcct ccctcaccac
     2341 agcattcggc tgtgctggcg acaaattgcg cataagcctg accttgctca tttaattcag
     2401 caagatagtc acgccattca catggagaat aacgaaacca gcggtgataa aaccctgcca
     2461 accgagcggc atgaccgttc tcgatcattc ggtgaattgt gcgcagcagg tcatcacggt
     2521 catcaattcc ccatgaggaa gaaaggtcaa aaaagcatgt acctaaatac attttggggg
     2581 atgaatgcga aggtaaacag cattcattgc gtatttccat cggagtggaa agtgcaaaca
     2641 atattttttg cgaccccatg tccatataat ttatccttaa acattcattt aatgaatgca
     2701 aactacattt cgagactcag ttatttatac ttttctgacg caacataata cgcataaaat
     2761 aaggattacg caaaaatgga acacataaaa atataatata ttttatctac aaccataagg
     2821 aaatgataat aatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg
     2881 aacgtgctga aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg
     2941 atagcgcaat tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgctg
     3001 agcaaggtca ttgtgaagca cagtattcct tagggcaaaa gtataccgag gataaaagcc
     3061 gtcataaaga taatgagcaa gccatcttct ggctgaaaaa agctgcccta caaggccata
     3121 ctttcgcttc caacgccctt ggctggatac tggatcgtgg agaagacccc aactataaag
     3181 aagcggttac ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc
     3241 ttggatggat gtacagaaat ggcaacggag tcgcacaaga ctatacgctg gcatttttct
     3301 ggtacaaaca agctgcatta caaggccata gttacgcaca agacaatctg gccgatcttt
     3361 ataaagacgg agaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa
     3421 gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg
     3481 aaggggtgga ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg
     3541 aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga
     3601 aagattatca ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg
     3661 cctggtataa cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac
     3721 aggctctcga cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa
     3781 ttcgtgagac tgaagcttta ctgtagaaat aaaaacaagg gccggggaat ttcattctgc
     3841 ccatattctc tttattgcta tgcgaaatca gactattacg gctcaaacgg gcgtctctgg
     3901 aactggtcat aaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga
     3961 tggaagtgac atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa
     4021 accccatgat gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg
     4081 atgtctgcca gctcctgcca caagctttct ttaatcaccc gcataaagac gctgtcagat
     4141 tcttctttca ggctctcttc atagctcatg gcgaactctt ccaggtcacg tctgacagct
     4201 cgcgtcagct catcgatctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc
     4261 gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gttcagtgac
     4321 gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta
     4381 actgactata ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg
     4441 taaattaatg tacgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt
     4501 tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg
     4561 tagccgtatt gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga
     4621 tagaatccaa agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg
     4681 agagcaaaga gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca
     4741 tgggccacgt acgtaactac accatcggtg acgtgatcgc ccgttaccag cgcatgctgg
     4801 gcaaaaacgt tctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg
     4861 cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa
     4921 accagctcaa aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc
     4981 cggaatacta ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat
     5041 ataaaaagac ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac
     5101 aagttatcga cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc
     5161 agtggtttat caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg
     5221 atcactggcc agacaccgtt aaaaccatgc agcgtaactg gatcggtcgt tccgaaggcg
     5281 tggagatcac cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc
     5341 cggacacctt tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga
     5401 aagcggcgga aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag
     5461 ttgccgaagc tgaaatggcg acgctggaga aaaaaggcgt cgatactggc tttaaagctg
     5521 ttcacccatt aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggagt
     5581 acggcacggg cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct
     5641 ctaaatacgg cctgaacatc aaaccggtta tcctggcggc tgacggctct gagccagatc
     5701 tttctcagca agccctgact gaaaaaggcg tgctgttcaa ctctggcgag ttcaacggtc
     5761 ttgaccatga agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg
     5821 agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc tcgtcagcgt tactggggcg
     5881 cgccgattcc gatggtgacg ctggaagacg gtaccgtaat gccaaccccg gacgaccagc
     5941 tgccggtgat cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag
     6001 atccggagtg ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt
     6061 tcgacacctt tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag
     6121 aaggtatgct ggattccgaa gcggctaact actggctgcc ggtggatatc tacattggtg
     6181 gtatcgaaca cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg
     6241 atgcaggcat ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc
     6301 tggcagatgc cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg
     6361 atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc
     6421 atgaactggt ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc
     6481 cgcaggtgat ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt
     6541 ctccggctga tatgactctc gaatggcagg aatccggtgt ggaaggggct aaccgcttcc
     6601 tgaaacgtgt ctggaaactg gtgtacgagc acacagcaaa aggtgatgtt gcggcactga
     6661 acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaactatcg
     6721 ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta
     6781 tggagctgat gaacaaactg gcgaaagcac cgaccgatgg cgagcaggat cgcgctctga
     6841 tgcaggaagc actgctggcc gttgtccgta tgctcaaccc gttcaccccg cacatctgct
     6901 tcacgctgtg gcaggaactc aaaggcgaag gcgatatcga caacgcgccg tggccggttg
     6961 ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag
     7021 tccgtgccaa aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg
     7081 gccaggaaca tctggtagca aaatatcttg atggcgttac tgtacgtaaa gtgatttacg
     7141 taccaggtaa actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct
     7201 ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg
     7261 tgacaccacg caggttcctt ccactatgaa ggtcatgatc ctagactcag gcgatccgaa
     7321 cgggccatta agccgtgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga
     7381 taaagaaacc acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa
     7441 agataccgca tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt
     7501 gaatgcgacc gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg
     7561 ttcattcttc gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt
     7621 aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc
     7681 tgcggatatt cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc
     7741 tgcacgcgtc tccaccatgc tgggtaactg atgattcggt tgtacccgga acaactccgc
     7801 gcgcagctca atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta
     7861 ttgcaggaaa gccaggacgc tgttcgccag gtagctgcgg cacaaggatt cgaagaacac
     7921 cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct
     7981 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat
     8041 gcggcgatca atgaacaact tctcacactc accggacttc tgcatgacga cctgctgttg
     8101 atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgctctt
     8161 gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg
     8221 gttgctgtgc gcgcaaaaca gctcaactta gaactggatg acgcggcgaa tcaggtgctc
     8281 tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg
     8341 ctctggccag acggcaaact gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg
     8401 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcgcgttg
     8461 catattcttc agcaactgcg tctggaaggc agcgagccgg ttattttgtt gcgcacatta
     8521 caacgtgaac tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt
     8581 acgttgtttg ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta
     8641 aatcgcttaa gtcagccgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc
     8701 accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg
     8761 ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc
     8821 tctgtttggc ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct
     8881 ggcgaatttg attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg
     8941 tccccagccg gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccga
     9001 caagccatta tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc
     9061 acaaacactg aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat
     9121 tggtcaggat tcattgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa
     9181 tgcacatttg atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata
     9241 ccagcaatgg ctggaagatc atttgacaca taacccggaa gatcttcacc ttcagcctgc
     9301 cggtaaaatt tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga
     9361 acgtttgcaa aacggtgaat cgtgtgagga tttattgccg gaaccggtat tgacttacat
     9421 taaccaacaa ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg
     9481 ttaattcgtc atggcgaaac acaagcgaat gtcgatggtc tgtacagcgg tcatgcgccc
     9541 acacccctga ccgcacgcgg tattgagcaa gcgcaaaatc tgcatacact gctagatgat
     9601 gtttcctttg acctggtttt atgcagtgaa ctggaacggg cacagcatac cgcgcgactg
     9661 gttctcagtg accgccagct ccccgttcat atcatccctg aactcaacga aatgtttttt
     9721 ggcgactggg agatgcgaca tcatcgcgac ctcatgcaag aagatgccga aaactatagc
     9781 gcgtggtgca atgactggca gcatgctatc cccacgaacg gtgaaggatt tcaggcattt
     9841 tcgcaacgtg tggaacgctt tatcgcaagg cttagtgaat atcagcacta tcagaatatt
     9901 ttaatcgtca gccatcaggg cgtactgagt ctgttaattg ctcgtttaat tggcatgcct
     9961 gccgagtcta tgtggcattt tcgcgttgac caggggtgtt ggagcgccat tgatatcaac
    10021 caaaaattcg cgacgctacg cgtcctcaat agccgtgcca tcggggtcga gaatgcatga
    10081 cttttctgtt tttttacggg taagccgcaa cgaccattga cagccccggg caggctgata
    10141 ttctccgcag ccagactttt tccgccagac acgactttgt agaaattgtt ttacaaaaat
    10201 ggcgatgcaa tctgcggcgc ggggtgggat gatagcccac tttcgaaagc cgattcggcg
    10261 acaattgtcc cgaaatcgcc tctggttcag gtatactgac agaccatttt tatctatttg
    10321 attcacccag ggggaaaact tgcagggtaa agcactccag gattttgtta tcgacaaaat
    10381 tgatgacctc aaaggtcagg acatcatcgc cttagacgtt caaggcaaat ccagcatcac
    10441 cgactgcatg atcatctgta cgggtacgtc cagccgtcat gttatgtcca ttgctgacca
    10501 cgttgtgcag gagtctcgcg cagcgggcct gttaccgctc ggcgtagaag gtgaaaacag
    10561 cgccgactgg attgtcgtgg atttgggcga tgtgattgtc catgtcatgc aggaagagag
    10621 ccgtcgcctg tatgaactgg aaaaactctg gagttaatgc gtgaagctgc aacttgtcgc
    10681 cgtgggaacg aaaatgccgg actgggtaca aaccggtttt accgagtacc tgcgtcgttt
    10741 tccgaaagat atgcccttcg agctgattga aattccggcc ggcaaacgcg gcaagaatgc
    10801 ggacatcaag cgcatactcg acaaagaggg tgagcagatg ttggcggccg caggcaaaaa
    10861 ccgcattgtc atcctcgata ttccaggcaa gccctgggat acgccgcagt tagccgctga
    10921 gctggaacgc tggaagctgg atggtcgcga cgtaagtcta ctgattggcg ggcctgaagg
    10981 gttgtcgcct gcctgtaaag cggcggctga gcagagctgg tcgctgtcgg cgcttaccct
    11041 cccccatccg ctggttcgcg tgctggtcgc agagagtctg taccgggcgt ggagcatcac
    11101 caccaaccat ccttatcacc gtgagtgata agggagcttt gagtagaaaa cgcagcggat
    11161 gaaactacag aactcttttc gcgactatac ggcagagtcc gcgctgtttg tgcgccgggc
    11221 gctggtcgcc tttttgggga ttttgctgct gaccggcgtg cttatcgcca acctgtataa
    11281 tctgcaaatt gttcgcttta ccgactacca gacccgctct aatgaaaacc gcattaagct
    11341 ggtgcctatc gcgcccagcc gcggcattat ctacgaccgt aacggtatcc ctctggccct
    11401 caaccgcact atctaccaga tagaaatgat gccggagaaa gtcgataacg tgcagcaaac
    11461 gctggacgct ttgcgcagcg tggtagatct gaccgatgac gatattgctg cattccgaaa
    11521 agagcgcgca cgttcacacc gtttcacctc tattccggtg aaaactaacc tgaccgaagt
    11581 acaagtagct cgctttgccg tcaatcagta ccgttttccg ggtgtcgaag ttaaaggcta
    11641 taaacgtcgt tactatcctt acggttcggc gttgacccac gtcatcggat atgtgtcgaa
    11701 aatcaacgat aaagacgtcg aacgcctgaa taatgacggc aaactggcca actatgcggc
    11761 aacgcatgat attggtaagc tgggcattga gcgttactat gaagacgtgc tgcacggtca
    11821 gaccggttat gaagaggttg aagttaacaa ccgtgggcgt gttatccgcc agttaaaaga
    11881 agtaccaccg caagccggac acgatattta cctgacgctg gatctcaaac tccagcaata
    11941 tattgaaacg ctgctggcgg gtagccgcgc agctgtggtt gttaccgatc cgcgtacagg
    12001 tggggtgctg gcgctggttt ccacgcctag ttatgaccca aacctgtttg ttgacggtat
    12061 ctccagcaaa gattattccg ccttgctgaa cgacccgaat acaccgctgg tgaaccgcgc
    12121 cacacagggg gtttatcctc ccgcgtctac agttaaaccc tatgtggcgg tttcggcatt
    12181 aagcgccggg gtgatcacgc gcaatacgac gctgtttgac ccaggctggt ggcaactacc
    12241 agggtcggaa aaacgttatc gtgactggaa aaaatggggc cacgggcgtc tgaatgtcac
    12301 aagatcgctg gaagaatctg cggatacctt cttctatcag gtggcctacg atatggggat
    12361 cgatcgcctc tccgaatgga tgggtaaatt cggttatggt cattacaccg gtatcgacct
    12421 ggcggaagaa cgttccggca acatgcctac ccgcgaatgg aaacagaaac gctttaaaaa
    12481 accgtggtat cagggtgaca ccattccggt tggtatcggc cagggttact ggacagcgac
    12541 cccaatccag atgagtaagg cactgatgat cctgattaat gacggtatcg tgaaggttcc
    12601 gcatttgctg atgagcaccg ccgaagacgg caaacaggtg ccatgggtac aaccgcatga
    12661 accgcccgtc ggcgatattc attccggtta ctgggagctg gcgaaagacg gtatgtacgg
    12721 tgttgctaac cgccctaatg gtacggcgca taaatacttt gccagcgcac cgtacaaaat
    12781 tgcggcgaaa tctggtaccg ctcaggtctt cggtctgaaa gcgaacgaaa cctataatgc
    12841 gcacaaaatt gccgagcgtt tacgtgacca caaactgatg accgcctttg cgccatacaa
    12901 caatccgcaa gtggctgtcg ccatgattct ggagaacggc ggcgcgggtc cggcggttgg
    12961 tacgctgatg cgccagatcc tcgaccacat tatgctgggt gataacaaca ccgatctgcc
    13021 tgcggaaaat ccagcggttg ccgcagcgga ggaccattaa tcatgacggc taatccgaat
    13081 aaaaaaacat tctgggataa agtccatctc gatcccacaa tgctgctgat tttactggca
    13141 ttgctggttt acagcgccct ggttatctgg agcgccagcg gtcaggatat tggcatgatg
    13201 gagcgtaaaa tcggccaaat cgcgatgggt ctggtcatca tggtggtgat ggcgcaaatt
    13261 cctccacgcg tttatgaagg ctgggccccc tatctctata tcatctgtat tattttgctg
    13321 gtggcggtag atgctttcgg agccatctct aaaggcgctc aacgctggct ggacctcggt
    13381 attgttcgtt ttcagccgtc ggaaattgcc aaaatagccg taccactgat ggttgcgcgc
    13441 tttatcaacc gcgacgtttg cccgccatcg ttgaagaaca ctggcatcgc gctggtgctg
    13501 atatttatgc ccacgctgct ggtggctgca cagcctgacc tgggaacatc aatcctcgtt
    13561 gcgctttccg gtctgtttgt actgttcctc tctggcctta gctggcgtct gattggcgtc
    13621 gcagtagtgc tggtagcggc gttcattccg attctatggt tcttcctgat gcatgattac
    13681 cagcgccagc gcgtaatgat gctcctagac ccggaatcag acccactcgg cgcgggctat
    13741 cacattattc agtctaaaat tgctattggc tccggcggat tacgcggcaa aggctggctg
    13801 cacggcactc agtcacagct tgaatttctc cccgaacgcc atactgactt tatcttcgcg
    13861 gtactggcgg aagagctggg attagtgggc attctgattc tgctcgctct ctacattctg
    13921 ctgatcatgc gcgggctgtg gatagccgcc agagcgcaaa ccacctttgg tcgcgtcatg
    13981 gctggtggct taatgctgat attattcgtt tatgtcttcg taaatattgg tatggtaagc
    14041 ggtattctgc cggttgtagg ggttccgctc ccactggtca gttatggagg atcggcgcta
    14101 attgtgctga tggctgggtt cgggattgta atgtcaatcc acacccacag gaaaatgttg
    14161 tcgaaaagcg tgtaagaggt gcgcaatgcg taagcagtgg ctcgggatct gcatcgcggc
    14221 aggaatgctc gcggcatgta caagcgatga tggtcagcaa cagacggtaa gtgtaccgca
    14281 gcctgcggta tgtaacggcc ccatagttga aattagcggg gcggacccgc gtttcgaacc
    14341 actgaacgcg acggcaaatc aggattacca gcgcgacggt aaaagctaca aaatcgtgca
    14401 ggatccgtct cgatttagcc aggcgggact ggcggcaatc tatgatgccg aaccaggcag
    14461 taacctgacg gcctctggcg aagctttcga tccgacaaag ctgacggcgg cccatccaac
    14521 gcttccgatc cccagctacg ccagaatcac taacctggct aacgggcgaa tgatcgtggt
    14581 gcgcattaat gatcgcggcc cttacggcaa cgaccgcgtt atttcgcttt ctcgcgcggc
    14641 agctgaccgt cttaacacgt caaacaacac caaagttcgt atcgatccga ttattgtcgc
    14701 ccaggatggt tcgctttctg gtcctggtat ggcttgtacc acagtcgcca aacagactta
    14761 tgccctgcct gcacctcccg atttaagcgg tggcgcggga acaagttcag tgtctggccc
    14821 gcagggtgac attcttccgg tcagtaattc gacgctaaaa agcgaagatc cgaccggcgc
    14881 gccggtaacc agtagcggtt tcctcggcgc tcaaacgacc ttagcgcctg gtgtactgga
    14941 aggcagcgaa ccgacgcctg cgccacagcc cgttgttaca gcttcgtcga caacgcctgc
    15001 aacctcgcct gcaatggtga caccgcaagc cgcctcgcaa agcgccagcg gcaactttat
    15061 ggtgcaagtc ggggccgtaa gcgatcaggc tcgtgcgcaa cagtaccaac agcaactggg
    15121 acagaagttc ggcgtccccg gtcgcgtaac tcaaaatggc gcggtctggc ggatccagct
    15181 tggcccattc gccagcaaag ccgaagccag taccttgcag caacgtttgc aaaccgaagc
    15241 ccaattacag tcatttatta ctaccgcgca gtagcgtaaa gcaggcatct gatgtgtcaa
    15301 tttctgtaaa tgacgttaac aaagtcacgc ggaaagtcag atgcctgccg gtagtgcatt
    15361 tgctatagta gggcactttt ttaattccat cacggatgtc gtagttcaga ccatgaatac
    15421 cattttttcc gctcgtatca tgaagcgcct ggcgctcacc acggctcttt gcacagcctt
    15481 tatctctgct gcacatgccg atgacctgaa tatcaaaact atgatcccgg gtgtaccgca
    15541 gatcgatgcg gagtcctaca tcctgattga ctataactcc ggcaaagtgc tcgccgaaca
    15601 gaacgcagat gtccgccgcg atcctgccag cctgaccaaa atgatgacca gttacgttat
    15661 cggccaggca atgaaagccg gtaaatttaa agaaactgat ttagtcacta tcggcaacga
    15721 cgcatgggcc accggtaacc cggtgtttaa aggttcttcg ctgatgttcc tcaaaccggg
    15781 catgcaggtt ccggtttctc agctgatccg cggtattaac ctgcaatcgg gtaacgatgc
    15841 ttgtgtcgcc atggccgatt ttgccgctgg tagccaggac gcttttgttg gcttgatgaa
    15901 cagctacgtt aacgcactgg gcctgaaaaa tacccacttc cagacggtac atggtctgga
    15961 tgctgatggt cagtacagct ccgcgcgaga tatggcgctg atcggccagg cattgatccg
    16021 tgacgtaccg aatgaatact cgatctataa agaaaaagaa tttacgttta acggtattcg
    16081 ccagctgaac cgtaacggcc tgttatggga taacagcctg aatgtcgacg gcatcaaaac
    16141 cggacacact gacaaagcag gttacaacct tgttgcttct gcgactgaag gccagatgcg
    16201 cttgatttct gcggtgatgg gtggacgtac ttttaaaggc cgtgaagccg aaagtaaaaa
    16261 actgctaacc tggggcttcc gtttcttcga aaccgttaac ccactgaaag taggtaaaga
    16321 gttcgcctca gaaccggttt ggtttagtga ttctgatcgc gcttcgttag gggttgataa
    16381 agacgtgtac ctgaccattc cgcgtggtcg catgaaagat ctgaaagcca gctatgtgct
    16441 gaacagcagt gaattgcatg cgccgctgca aaagaatcag gtcgtcggaa ctatcaactt
    16501 ccagcttgat ggcaaaacga tcgagcaacg cccgctggtt gtgttgcaag aaatcccgga
    16561 aggtaacttc ttcggcaaaa tcattgatta cattaaatta atgttccatc actggtttgg
    16621 ttaaaaattg aacacttgaa agtgtaattt ccgtccccat atactaagca tcagtaaaaa
    16681 aactcccacc ttctggcggg agttgctatt taattacgtt acgccggagc tgacatgaaa
    16741 accaaactta acgaactgct tgaattccct actcctttta cttacaaagt tatggggcag
    16801 gcgttacctg agctggttga tcaggtggtt gaagtggtac agcgccatgc gccaggtgac
    16861 tacaccccaa cggtaaaacc aagcagcaaa ggcaactacc actcggtatc tatcactatc
    16921 aacgccactc atatcgagca ggttgaaaca ctgtatgaag aactgggcaa aatcgatatt
    16981 gtccgcatgg ttctgtaact cgcttctccg ttacccggct cctggtcggg taactctcct
    17041 ctcctcgctg tgatatactt ttcccccact tttactcatt ctccacggag atgccgtttt
    17101 gtatcaggat aaaattcttg tccgccagct cggtcttcag ccttacgagc caatctcccg
    17161 ggctatgcat gaattcaccg atacccgcga taatagtacc cttgatgaaa tctggctggt
    17221 cgagcactat ccggtattca cccaaggtca ggcaggaaaa gcggagcaca ttttaatgcc
    17281 gggtgatatt ccggtgatcc agagcgatcg cggtgggcag gtgacttatc acgggccggg
    17341 acaacaggtg atgtatgtgt tgcttaacct gaaacgccgt aaactcggtg tgcgtgaact
    17401 ggtgaccttg cttgagcaaa cagtggtgaa taccctggct gaactgggta tagaagcgca
    17461 tcctcgggct gacgcgcctg gtgtctatgt cggggaaaag aaaatttgct cactgggttt
    17521 acgaattcga cgcggttgtt cattccacgg tctggcatta aacgtcaata tggatctttc
    17581 accattttta cgtattaatc cttgtgggta tgccggaatg gaaatggcta aaatatcaca
    17641 atggaaaccc gaagcgacga ctaataatat tgctccacgt ttactggaaa atattttagc
    17701 gctactaaac aatccggact tcgaatatat taccgcttaa ttccatacat caatggccca
    17761 atttacactg ggtcattact cataatacct gctaatgctt tacatctcct tcgcgtttgt
    17821 atattttctt tatcacttgc agaaatgcgg tgccacgcct tgctccgtaa ctcaccccat
    17881 tgattaacct attgaaatag ttgattatgg tgagcaaaag cgttccgttc caattagaca
    17941 taagattatt gtaatttttt aaatattatt tttccatgaa taaaattgga gtaagtgcgt
    18001 ggatagtaat aatcaaattg aaccctgttt aagcagaaag tcgtcagaag gtaaaccaca
    18061 aatatttaca accttacgaa atattgatct taaccttctg actatttttg aagctgtata
    18121 tgtacataaa gggatcgtta atgcagcgaa agtgcttaat ctgaccccct cggcaatcag
    18181 tcagtctatt cagaaactgc gcgttatatt ccctgaccca ttgtttattc gcaaaggcca
    18241 gggtgtcact cctaccgcat ttgcgatgca tctacatgag tatatcagtc agggccttga
    18301 gtccattctt ggcgcgctgg atatcgaagg aagctatgat aagcaacgaa cgataactat
    18361 tgcgaccact ccctcggtcg gagccctggt ccttcctgtc atctatcggg cgattaaaac
    18421 tcactatccg cagcttttac tgcgcaaccc gcccatcagc gacgcggaaa accaactcag
    18481 tcagtttcaa accgatctca tcatcgataa catgttttgc accaatcgta cggtgcaaca
    18541 tcatgttctg ttcaccgaca atatggtgtt aatttgccgc aagggaaatc cactactctc
    18601 tttagaagat gacagagaga ctatcgacaa cgctgcgcat gtacttctgt taccggaagg
    18661 gcaaaatttc agcggtctgc ggcagagagt tcaagagatg tttccggacc ggcaaatcaa
    18721 tttcaccagc tacaacattt tgacaatcgc tgcactggtt gccaacagtg acatgttagc
    18781 gattattccc agccgttttt ataacctgtt tagtcgttgc tggccgctgg aaaaactgcc
    18841 ttttccgtcc ttaaatgagg agcaaataga tttctctatc cactacaaca aattcagcct
    18901 gcgtgatccc atcctgcacg gagtaataga tgttatccgt aatgcatttt gaaaaagtat
    18961 tgttacagcc agatcacagt gcagaacgat aaaatggcac aatccaacaa caattttaca
    19021 ttttcgcgac cgctttggct gctttcaggt ccgtttcaat gatatactgc cagtcgttaa
    19081 ttcaaaaata gttgataatt acaacaatct attgaattga aacgctttcc ttcgtaattc
    19141 gcaactggaa cacgcacgct atgagtaaac ccattgtgat ggaacgcggt gttaaatacc
    19201 gcgatgccga taagatggcc cttatcccgg ttaaaaacgt ggcaacagag cgcgaagccc
    19261 tgctgcgcaa gccggaatgg atgaaaatca agcttccagc ggactctaca cgtatccagg
    19321 gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt ctgcgaggaa gcctcctgcc
    19381 ctaacctggc ggaatgcttc aaccacggca cagcaacgtt tatgatcctc ggcgctattt
    19441 gtacccgccg ttgtccgttc tgtgatgttg cccacggtcg cccggtagct cctgatgcca
    19501 atgaaccagt gaaactggcg cagaccattg ccgatatggc gctgcgttat gtggttatca
    19561 cctccgttga ccgtgatgac ctgcgcgatg gcggtgccca acactttgcg gattgtatta
    19621 ctgccattcg ggaaaaaagc ccgcaaatca aaattgaaac tctggtgccg gatttccgcg
    19681 gtcgaatgga tcgtgctctg gatattctga ctgcaacgcc cccagatgtg ttcaaccata
    19741 acctggaaaa cgtaccgcgt atttaccggc aggtacggcc tggcgcagat tacaactggt
    19801 cgctgaagct gctggaacgc tttaaagaag cgcatccgga aatcccgacc aagtctggtc
    19861 tgatggtggg tctgggtgaa accaatgaag aaattattga ggtaatgcgc gacctgcgcc
    19921 gtcatggtgt gacgatgtta acgctggggc aatatttgca gccaagccgc catcacctgc
    19981 cggttcaacg ttacgttagc ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga
    20041 tgggctttac ccatgctgca tgcggtccgt ttgtccgctc ttcttaccac gccgatttgc
    20101 aggcgaaagg gatggaagtt aagtaatcct gtaataatta cttacatctg tctgataaaa
    20161 aacccgcttt ttgaagcggg ttttttgtat cgaacaagat attgagggag cgtcctgctc
    20221 gccacgtcac tctttatgag agagcttttc agcctgaaga tcaacgtctg cgcctttttt
    20281 cgccgcagca tcgtcatcat tcatcgcctt cttgaaccct ttaatggccg ctccaaggtc
    20341 tccgcccagc gtacgtaact tcttagtccc aaacagcaga acgaccagcg ccgcaactac
    20401 cagcagtttg gtaatactaa tctcacccat agataccttc ttgacatata accggcaaca
    20461 gaacgcgcct taaaaaccag agaatattaa cggtcattcg acgcgcgcac acaatagcaa
    20521 tctgtaacaa ggtgaatcaa gcgccgagta aaaaaacatc ataataattg cggcggcgca
    20581 aagcgacggt tgtttaagat gggcagttgc gcgcgcacct gacgcacacg ttcgggcgtc
    20641 acttccgcca taatgagtgc aggcatttct gacgctgccg caatggtgac gccaaaggga
    20701 tctataatcc ggctttgccc gatatttttg ttcccgcact cccccgccgc caccatataa
    20761 caggtggtat ccagcgcacg agcggcaagc aacgttgacc agtgatgctc tttgagcggc
    20821 ccacgaaccc aggcggcagg aagtaccagg atttcagctc cctgtaaagc ctgtgccagc
    20881 gccagctctg gaaagcgtaa gtcataacag gtcatcagac cgaccttcat cccttccacc
    20941 tccagtaacg gagctatttc attaccagca tcaacatggc gtgattcctg aatggcaaat
    21001 gcatcataga gatgcagttt ggcataacgg gcgacgatgt ttcctgcctg aagtgccacc
    21061 agcatattcc atgcgcgccc cggcgttgaa ggaagatgaa tcgtcagaat tgtcgtcatc
    21121 atgttacgtt tactttctcg ccgtaaacgt ccgaggaatt cgccttccag cagctgtgct
    21181 gatttaaccg atagatctgc atcatgatca tcgcgcgcca gcaatgcttc cggcaggaca
    21241 aacagcgata cgtcgttttc cgccgcctgc gccatcaacg aggcacaaat ctcagcgttc
    21301 ttttcccaca cagatgtaac agcaaactgt cctgcagcaa ccagcattgc cacctcctgt
    21361 cagagtcgta aaattcgcca acgacgatta ttcggcgtta cacttatcac tcatacaaat
    21421 caaatagcag gattttgcag tgttacaact tcttttagca gtttttattg gcggtggtac
    21481 gggaagcgta gcgagatggc tgttaagtat gcgatttaac ccgctgcatc aggcgattcc
    21541 gttggggacg ctggcagcaa acctgattgg ggcattcatc ataggaatgg gattcgcctg
    21601 gttcagcagg atgacgaaca ttgatccagt gtggaaagta ttaatcacca ccggattttg
    21661 tggcggtcta acaaccttct caacattttc ggcagaagtg gtgtttttgt tacaagaggg
    21721 ccgctttggc tgggcattac tgaacgtttt cgtcaacctt ctggggtctt ttgccatgac
    21781 cgcactggca ttctggctgt tttcggcctc aaccgcacac taaagaccaa aaaaaacccg
    21841 ctgattaagc gggttttgaa ttcttgctga cgtatcttac agagcgatta cgtttgcagc
    21901 agaagggcct ttggcaccgt tagtgatttc gaactctacg cgctgacctt cagcaagagt
    21961 tttaaaacca ttagtctgga ttgcagagaa gtgtacgaac acgtctttgc tgccgtcttc
    22021 cggagtaatg aaaccgaatc ctttggactc attaaaccac ttaacgttac ctttaatctt
    22081 agacatcaaa attaccttta catgaaaaat agacacaaat gctgtgtcgg ttatcagtac
    22141 accaattgtg gtacgctttg tccagtgcaa ctttactaaa aagtgataaa agtcgcgtta
    22201 tttttgatgt tgcttatttt agttattgat tttaaagaag ttactaaaac ttcatttgcc
    22261 tcaaaactga aagcgcatcc aggcaaagta cacattgcca ttgttgtagg tacccggaat
    22321 gtaggtcatc tgaaaagtca ctgggccata acccacggag gccaatggca gtagaaccgg
    22381 gagagggatg taattccagt tatcgcgtgc cgttacgcca gcggtgaatc ccagacctaa
    22441 atgaaaattt tcatccgcca gcggtcgcca ggtactttcc catccgtatc cggcaatcgg
    22501 ttcccatttg ttccacgagt ccttaaatgc catggcatac aggccatgcc agtttccttt
    22561 ttcatcccaa cgcgacaggc caaaaccgcc accccacggt cgctcgttat agcgatcggt
    22621 tttttctttg tcgtaagcga aacgtgcatg ccaggtgatg gcaggaatat ataaatcata
    22681 atgttcaggc tgttgccagg tttgtgcaat attttctcta aacgttgtca tcctctcatc
    22741 tgcgttagca aaaactttac caacgctgat taactgaata aaaacaaagg aaaagatagc
    22801 gacatattta ctcacgttca tttgtgacca taaaacattt atcaaaaatc tactactagc
    22861 atagcaaagc tacaattaac ataaccttaa tagacccaac ataaagaata atctgaatag
    22921 ctgctctatt tatttccgct tgttcaagtt aaatatttcc gcgcatcgat taaagatgat
    22981 gcgtcgtttt ctgcgatggg aatagtcaaa aaagaaaaac caagtctttt ttgatgacaa
    23041 aatgcaatca aggaaaaatt attttatttt tttaacgaga tacaacaatc atcttaacga
    23101 agtatataat atatatcgtc aacccatgct catattagat tattttttga gcgcacagcc
    23161 gcagctaaca ccattgcaat taacaaattt gcatcaatcc accatcaatt tacacacatt
    23221 attatgtgat aattgccaac cgctaaatat gcgttttgtt atctatgtat aaaaacagca
    23281 acttcaatgt cttaatggca gtttttcttg attttaatca gcattcatcg ccaatttatt
    23341 gggcatattt tttccttaag ctttaggaag tttttattat ttactttggg cctggagaca
    23401 ggaaaaatta tgctgacatt cattgaactc cttattgggg ttgtggttat tgtgggtgta
    23461 gctcgctaca tcattaaagg gtattccgcc actggtgtgt tatttgtcgg tggcctgtta
    23521 ttgctgatta tcagtgccat tatggggcac aaagtgttac cgtccagcca ggcttcaaca
    23581 ggctacagcg ccacggatat cgttgaatac gttaaaatat tactaatgag ccgcggcggc
    23641 gacctcggca tgatgattat gatgctgtgt ggctttgccg cttacatgac ccatatcggc
    23701 gcgaatgata tggtggtcaa gctggcgtca aaaccattgc agtatattaa ctccccttac
    23761 ctgctgatga ttgccgccta ttttgttgct tgtctgatgt cactggccgt ctcttccgca
    23821 accggtctgg gtgttttgct gatggcaacc ctgtttccgg tgatggtaaa cgttggtatc
    23881 agtcgtggcg cagcagctgc catttgtgcc tccccggcgg cgattattct cgcaccgact
    23941 tcaggggatg tggtgctggc ggcgcaggct tccgaaatgt cgctgattga cttcgccttc
    24001 aaaacgacgc tgcctatctc aattgctgca attatcggca tggcgatcgc ccacttcttc
    24061 tggcaacgtt atctggataa aaaagagcac atctctcatg aaatgttaga tgtcagtgaa
    24121 atcaccacca ctgcccccgc gttttatgcc attttgccgt tcacgccgat catcggtgta
    24181 ctgatttttg acggtaaatg gggtccgcaa ttacacatca tcactattct ggtgatttgt
    24241 atgctgattg cctccattct ggagttcctc cgcagcttta atacccagaa agttttctct
    24301 ggtctggaag tggcttatcg cgggatggca gatgcgtttg ctaacgtggt gatgctgctg
    24361 gttgccgctg gggtattcgc tcaggggctt agcaccatcg gctttattca aagtctgatt
    24421 tctatcgcta cctcgtttgg ttcggcgagt atcatcctga tgctggtatt ggtgattctg
    24481 accatgctgg cggcagtcac gaccggttca ggcaatgcgc cgttttatgc gtttgttgag
    24541 atgatcccga aactggcgca ctcttccggc attaacccgg cgtatttgac tatcccgatg
    24601 ctgcaggcgt caaacctggg tcgtacccta tcacccgttt ctggcgtagt cgttgcggtt
    24661 gccgggatgg cgaaaatctc accatttgaa gtcgtaaaac gcacctcggt gccggtgctt
    24721 gttgggctag tgattgttat cgttgctaca gagctgatgg tgccaggaac ggcagcagcg
    24781 gtcacaggca agtaaatagt aatgccggag gcaagtttcc tccggcttta tcatgtcacc
    24841 cactgtggta tatgcgttgt ggtctgccaa ctttgccgtg aacaatttca gcaataatca
    24901 gatggcggct ggcgcaatat tcaagataac gcctggcagt ggtgcggctg atggtcagtg
    24961 cctgcgccac cgtttctgcc gtatgttgca caccaggctc tttaaacagt tttcgcaccg
    25021 cgtttagcgt caaggggtca atgccggtcg gcagttcgtc tttgggttca ccgcgagcat
    25081 aggcattaaa catctcatca atttgcttct ggctggcgct atcaatactt tccagcatat
    25141 gtttacactg gcggaaacgg gttagcgttt gccccagtgt attgattcac ttgaagtacg
    25201 aaaaaaccgg gagaacattg gattattcgg gatctgatgg gattagattt ggtggggctt
    25261 gcaagcctgt agtacaaatt ttaggcgtta accaatgaaa cgcgaaagat agtaaaaaat
    25321 tgcttttgtt tcattgaaaa aac
//
LOCUS       DAJTYF010000047        24990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTYF010000047 DAJTYF010000000
VERSION     DAJTYF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.047"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            27..2294
                     /gene="adiA"
                     /locus_tag="OO590_002084"
     CDS             27..2294
                     /gene="adiA"
                     /locus_tag="OO590_002084"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6637451.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2619..3380
                     /gene="adiY"
                     /locus_tag="OO590_002085"
     CDS             2619..3380
                     /gene="adiY"
                     /locus_tag="OO590_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6637452.1"
                     /translation="MRICSDQPCIVLLTEKDVRIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3517..4854
                     /gene="adiC"
                     /locus_tag="OO590_002086"
     CDS             3517..4854
                     /gene="adiC"
                     /locus_tag="OO590_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6637453.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLAPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4958..6601
                     /gene="eptA"
                     /locus_tag="OO590_002087"
     CDS             4958..6601
                     /gene="eptA"
                     /locus_tag="OO590_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6637454.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6598..7266
                     /gene="basR"
                     /locus_tag="OO590_002088"
     CDS             6598..7266
                     /gene="basR"
                     /locus_tag="OO590_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6637455.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7267..8367
                     /gene="pmrB"
                     /locus_tag="OO590_002089"
     CDS             7267..8367
                     /gene="pmrB"
                     /locus_tag="OO590_002089"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6637456.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="OO590_002090"
     CDS             complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="OO590_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6637457.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8544..10046)
                     /gene="proP"
                     /locus_tag="OO590_002091"
     CDS             complement(8544..10046)
                     /gene="proP"
                     /locus_tag="OO590_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6637458.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10310..11188)
                     /locus_tag="OO590_002092"
     CDS             complement(10310..11188)
                     /locus_tag="OO590_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6637459.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNSGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="OO590_002093"
     CDS             complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="OO590_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6637460.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13815..14150
                     /locus_tag="OO590_002094"
     CDS             13815..14150
                     /locus_tag="OO590_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6637461.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14308..14751
                     /gene="yjdN"
                     /locus_tag="OO590_002095"
     CDS             14308..14751
                     /gene="yjdN"
                     /locus_tag="OO590_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6637462.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14884..15672
                     /gene="phnC"
                     /locus_tag="OO590_002096"
     CDS             14884..15672
                     /gene="phnC"
                     /locus_tag="OO590_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6637463.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15697..16713
                     /gene="phnD"
                     /locus_tag="OO590_002097"
     CDS             15697..16713
                     /gene="phnD"
                     /locus_tag="OO590_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6637464.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSSGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16819..17598
                     /gene="phnE"
                     /locus_tag="OO590_002098"
     CDS             16819..17598
                     /gene="phnE"
                     /locus_tag="OO590_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR6637465.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17619..18344
                     /gene="phnF"
                     /locus_tag="OO590_002099"
     CDS             17619..18344
                     /gene="phnF"
                     /locus_tag="OO590_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR6637466.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18345..18797
                     /gene="phnG"
                     /locus_tag="OO590_002100"
     CDS             18345..18797
                     /gene="phnG"
                     /locus_tag="OO590_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR6637467.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMIRGDNA"
     gene            18794..19378
                     /gene="phnH"
                     /locus_tag="OO590_002101"
     CDS             18794..19378
                     /gene="phnH"
                     /locus_tag="OO590_002101"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR6637468.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19378..20442
                     /gene="phnI"
                     /locus_tag="OO590_002102"
     CDS             19378..20442
                     /gene="phnI"
                     /locus_tag="OO590_002102"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR6637469.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20435..21280
                     /gene="phnJ"
                     /locus_tag="OO590_002103"
     CDS             20435..21280
                     /gene="phnJ"
                     /locus_tag="OO590_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR6637470.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21277..22035
                     /gene="phnK"
                     /locus_tag="OO590_002104"
     CDS             21277..22035
                     /gene="phnK"
                     /locus_tag="OO590_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR6637471.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22146..22826
                     /gene="phnL"
                     /locus_tag="OO590_002105"
     CDS             22146..22826
                     /gene="phnL"
                     /locus_tag="OO590_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR6637472.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22823..23959
                     /gene="phnM"
                     /locus_tag="OO590_002106"
     CDS             22823..23959
                     /gene="phnM"
                     /locus_tag="OO590_002106"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR6637473.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPTSLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            23959..24516
                     /gene="phnN"
                     /locus_tag="OO590_002107"
     CDS             23959..24516
                     /gene="phnN"
                     /locus_tag="OO590_002107"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR6637474.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLLVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24503..24937
                     /gene="phnO"
                     /locus_tag="OO590_002108"
     CDS             24503..24937
                     /gene="phnO"
                     /locus_tag="OO590_002108"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR6637475.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
BASE COUNT         5936 a         7007 c         6533 g         5514 t
ORIGIN      
        1 attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc gagtttctcc
       61 atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta agccagcaaa
      121 atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc tcttcaaacg
      181 aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa catcaaaacg
      241 tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc ttcctgttgg
      301 gcgatcggga aaaagccctc gccgcaatgg accgggatct gctggagctt gtcgatgaat
      361 tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgccgtt gccgcgatga
      421 cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa tatagtgaca
      481 tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt accaaaacac
      541 ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc gacatgggca
      601 tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc gaaagcgaaa
      661 aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc ggtacttccg
      721 gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg gtcgttgacc
      781 gtaactgcca taaatccatc gaacaaggtt tgatgctgac aggcgcgaaa ccggtctata
      841 tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag gaaatgcaac
      901 ctgaaacctt gcaaaagaaa atcagtgaaa gcccgctgac caaagacaaa gccgggcaaa
      961 aaccgtctta ctgcgtggta accaactgca cctatgacgg cgtgtgttat aacgctaaag
     1021 aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa gcctggtacg
     1081 gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc gaacctggcg
     1141 atcacaacgg tcctaccgtt ttcgccaccc actccatcca caaactgctg aatgcgctgt
     1201 cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc tcccgcttca
     1261 accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc gcatccaacg
     1321 acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacacag gaagtgatta
     1381 acgaagcggt tgatttccgt caggcgatgg cgcggctata taaagagttc accgctgacg
     1441 gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa accggcaaaa
     1501 cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac tgctgggtaa
     1561 tacatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg agtatgctcg
     1621 acccaattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa ctggaagaaa
     1681 ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt gtgcctaccc
     1741 gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt gggaaatggg
     1801 ggactctggt taacaccctt tgctccttca aacgccacta tgacgccaac acaccgctgg
     1861 cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac atggggattc
     1921 acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc gcacggttga
     1981 acgaagccta ttccggcctg ccggtggcgg aaatcacccc gcgtgaagcg tacaacgcga
     2041 ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc atcgcggcaa
     2101 actcagttat cccgtatccg ccaggaatcc cgatgctgct gtctggtgaa aacttcggcg
     2161 ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac caccatttcc
     2221 ctggatttga acacgaaact gaagggactg aaattattga cggtatttac cacgttatgt
     2281 gcgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac attacgccgc
     2341 ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca aacaaaaatg
     2401 tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta tcctgtaaaa
     2461 aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact cgttattttt
     2521 atgcatgttt atattcatca tataattata taaccatttc ccggtatcgc tttgctttag
     2581 cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc agcgaccaac
     2641 cttgtattgt tttattgact gaaaaagatg tccggataag ggtgaatggg aaagaaccta
     2701 ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt atcgacgtct
     2761 cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa gactacctcc
     2821 ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct acgcccatac
     2881 tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac agcatgatgc
     2941 ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg gtgctatccc
     3001 gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt aattgtgtaa
     3061 gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat cttagtatgg
     3121 tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagttgaaa agcgaaaaca
     3181 ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat gaattaatga
     3241 tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt acgtcgtact
     3301 ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt agtcagcaca
     3361 gagaacgcac tgtcgcctga tttttaacct taacgaagag ctatattaat aacggcatca
     3421 gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata tgacgtaatt
     3481 tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc tgatgctcac
     3541 aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg gtcaggtgtt
     3601 tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg atggttggtg
     3661 acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt cctcgcccca
     3721 agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct cggttatcaa
     3781 accaacgtcc tctactggct ggcctgctgg atcggcaata tcgccatggt ggtcattggc
     3841 gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt aaccatcacc
     3901 tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa aatgatcacc
     3961 cgcgtgcagg ccgttgccac tgtgctggcg ctgatcccca tcgtcgggat tgccgtattt
     4021 ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag cggcctgggc
     4081 accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat cggtgtggaa
     4141 agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc tatcgccacc
     4201 attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc gattatgggg
     4261 atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc cgcacggatg
     4321 gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg ttgcttaggt
     4381 tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc cgatgacgga
     4441 ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc ggggctgatt
     4501 atcgtcggta ttttgatgac catcttccag ctcagcagca tttcaccaaa cgcgaccaaa
     4561 gagttcggtc tggtttcttc cgtctcggtc atctttacac tggtgccata tctttacacc
     4621 tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc ggcatatctg
     4681 gctgttacta ccattgcctt cctctactgc atttgggccg tggtggggtc cggggcgaaa
     4741 gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc gctgaattac
     4801 aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga ttaattcccc
     4861 ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa ttttgctttg
     4921 cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc tactaaaaag
     4981 accctctttg aatttactcg cctggctatt gttggccgct ttttatatct ctatctgcct
     5041 gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc tgcataacgt
     5101 actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg tcctgacact
     5161 aagctctttc ttatggctta atcgaacact ggcctgcctg tttattctgg ttggcgcggc
     5221 tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga ttgccaatat
     5281 tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt tattaacgct
     5341 gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca aacctgccac
     5401 ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg tactgctgat
     5461 tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca ataacaaaga
     5521 gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat ggtactccca
     5581 tcagcgactg gcaaatctgc cgctggtgcg aattggtgaa gacgcgcacc gcaacccgtt
     5641 aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa cctcgcgggc
     5701 ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg cgaaagataa
     5761 cgtggtctat ttccctaata ccgcatcttg cggtacggca acggcagttt cagtaccgtg
     5821 catgttctcg gatatgccgc gtgagcacta caaagaagag ctggcacagc accaggaagg
     5881 cgtgctggat atcattcagc gagcgggcat caacgtgctg tggaatgaca acgatggcgg
     5941 ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga atctgcctga
     6001 tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg aagagtacat
     6061 caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc acggtccgac
     6121 ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg acaccaatga
     6181 gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc tggtttacgt
     6241 cgactatatt gttgataaag cgattaatct gcttaaagaa catcaggata aatttaccac
     6301 cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca tctatctgca
     6361 cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccaatgc tgctgtggct
     6421 gtcggaggat tatcaaaagc ggtatcaggt tgaccagaac tgcctgcaaa aacaggcgca
     6481 aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa ctggcgttga
     6541 gacgaagtat taccaggccg cggatgatat tctgcaaact tgcaggagag tgagtaaatg
     6601 aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct ggcggcgcaa
     6661 accgaaggct acgcgtgcga tggcgtgaca accgcgcgga tggcggaaca aagccttgag
     6721 gacggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga tggactgcat
     6781 tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct caccgctcgc
     6841 gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta tctggtgaag
     6901 ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg ccataataat
     6961 cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg ccgtcaggta
     7021 tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct gtcacggtta
     7081 atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat ctataactgg
     7141 gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg cgacaaagtg
     7201 ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc gaatgaggaa
     7261 aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca acggctgata
     7321 ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg gctatggcat
     7381 gaaagtaccg agcagattca actgtttgag caggcgctac gcgacaatcg caacaacgat
     7441 cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc cggcgtcttt
     7501 atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac ccgcccgctg
     7561 gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc cattgccatt
     7621 cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct ggtcagtcgc
     7681 ctgaccagca cgctggataa cgaaaggttg tttaccgctg acgtcgcgca cgaactgcga
     7741 acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca tcacattgat
     7801 gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca gctgctgcaa
     7861 ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa actgctggaa
     7921 gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg acagcaaacc
     7981 ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac cctgctgcgg
     8041 atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccgcaagg cagcaacatt
     8101 atgattaagc tgcaagaaga tggcggagcg gtcatggcag ttgaagatga aggaccaggt
     8161 attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga cagccgttat
     8221 ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca tcacgggcag
     8281 tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg gctgaagaaa
     8341 gatcagaacg tggcaaacca gatataaaga aagctgctga ccaccagcac gctgaacacg
     8401 gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa cctggaggag
     8461 agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat aagacagcgt
     8521 cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg caccaggcgg
     8581 gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat tatcgatttt ctgctcgata
     8641 ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc cgccggtgtc
     8701 gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat taaaccaatc
     8761 accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct ttcgaccagc
     8821 caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa tgccgccgcc
     8881 agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc cataacgccc
     8941 ataaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag gccgatgacg
     9001 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagtgcaac actaccaagt
     9061 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
     9121 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
     9181 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
     9241 attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
     9301 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
     9361 tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
     9421 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
     9481 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
     9541 tccagccagc tgcccataaa gccacgttta cggtcggggg agtattccgc aacaaatatc
     9601 gacgccccgg tatattcacc accgaccgag aaaccttgtg ccatcttaca gatcaacagc
     9661 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
     9721 ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata tttatcgccc
     9781 aacataccaa agaagagtcc gccaagcggt cgaatcagaa agggaacgga gaaagtggca
     9841 agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt acctaatgcg
     9901 taagcaacaa aaccataaac accaaaatca aaccattcca ttgcattacc cagtgatgct
     9961 gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg aagggtaatc
    10021 ggttttactt ttttcctttt cagcatagct ttcctcgcag agttgaccca tttccgcaaa
    10081 cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg atgcaatctc
    10141 tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca atcaaaccag
    10201 ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta aatttgtgat
    10261 caacttcaca catggatttc gtttaggact cattgatgta actgattatt tacgccaaat
    10321 ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg
    10381 gcatccccag cgcgacagtt tgccagcgtt gggtcactga ccagcaaacg ggttgtttat
    10441 ctgccgtggt ccactttccc agccaactta aaaaatcagc atgatccccg cccgtcggaa
    10501 cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac
    10561 tctctgccag atgttgtgtc gctttctcgc gcaaatcacc cagcaacaac accaccgctt
    10621 gcgggcttcc cagacggcag gcatcgccaa atgcacccca gcgcaactcc tccagaacga
    10681 taaaacagcg cccaggcaac gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa
    10741 tttgctcgct ctgatcacct gcctgcacca gtaggcctcg ttgcgttaac gccttttcct
    10801 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
    10861 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
    10921 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
    10981 gcagactaag cggcgcattt aacatgccgc tgttcgtcca tgtctgaagc tgcacacgcg
    11041 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttga ggatggcggg
    11101 catcatcgcc ctgcgccaga tccactaaaa agcgcggatg aaccgactcc agcacgcgac
    11161 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
    11221 atcgcgtaac agctgagcgt cggtgtaaat ccagctgtga gtcatcacag tctgctgcaa
    11281 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca gactttcgcg
    11341 taaacgcgtc tgtaattctg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc
    11401 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaatagag
    11461 atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga
    11521 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
    11581 aacgcctaaa cgcgtgctct gctcgtccgt tgcttcctgg cgcgaaatga cttcactgaa
    11641 cagctggcga gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat gaaacccagg
    11701 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatggggc gaataacatc
    11761 ggttaacacc cggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact
    11821 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg gtacgaatct cgcttaacgg
    11881 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc accgcatctt gctgttgacg
    11941 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
    12001 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
    12061 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
    12121 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaatg aggcgttagc
    12181 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
    12241 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
    12301 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
    12361 gttgttggct aactcatagc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg
    12421 gaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
    12481 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
    12541 cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
    12601 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
    12661 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
    12721 ctgaccggga taactttcca gccccgccag atggacaaac tccacttcaa tcacgggaat
    12781 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
    12841 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
    12901 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
    12961 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
    13021 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
    13081 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
    13141 accaacaatg gcattaatgg tggttgattt ccctgctttc atggtaccga caattgccag
    13201 caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc
    13261 gatattaggc tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag
    13321 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
    13381 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
    13441 gttactttta tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca
    13501 agaaataatt ttatatttag tgtgttgttt tttatcagaa taaataacgt cttctgatgc
    13561 gtttaaaacg tcagaaagat aaaaatatca tgtaaataaa aaaaagaaca agtagagcat
    13621 taacattatc ttaaataata aatagaggca aaaagattat tttctttttg cgtttccttt
    13681 caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca gggattttgt
    13741 gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc ctgctgactt
    13801 ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa tacacttacg
    13861 aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac gcagaacctg
    13921 cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg gctgacggcg
    13981 acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg ctgaaaattg
    14041 gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat tgcaaaatcg
    14101 acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga ttgtattgtg
    14161 atcggtaagc cgaataaggc gctcgcgccg catccggcaa cgatgccgac tgcctgatgc
    14221 gacgcttgcg cgtcttatca ggcagcctct catctgtata aatttcgaac tacacttaac
    14281 tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc ttttgccggt
    14341 aactgttccg acgcgattgc ctattatcaa cgtacgttgg gcgcggaact gctctataaa
    14401 atcagcttcg gcgaaatgcc gaaatcagcg caggacagcg ccgagaactg cccttccgga
    14461 atgcaatttc ccgataccgc catcgctcat gccaacgtgc gcattgccgg aagcgacatc
    14521 atgatgagcg atgctatacc gtcaggaaaa gccagctact ccggctttac gctggtgctc
    14581 gattcgcaac aggtcgaaga aggaaaacgc tggtttgata accttgccgc taacggaaaa
    14641 atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt caccgataaa
    14701 tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata acccgccggg
    14761 aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt tagtcacttt
    14821 taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta gggaggctgc
    14881 atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca gcatcaagcg
    14941 ctgcatgcgg ttgatctgaa cattcatcac agtgaaatgg tggctctgct tgggccgtcg
    15001 ggttccggca aatccaccct tttacgtcat ttaagcggtt tgattaccgg cgataaatcc
    15061 gccggtagcc atatcgagct actgggccgc actgtccagc gcgaaggccg cctggcccgc
    15121 gatatccgca aaagccgcgc caacaccggc tacatctttc aacaattcaa cctggtgaac
    15181 cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc gttctggcgc
    15241 acctgtttta gctggttcac cggcgagcag aaacagcgcg cgttacaggc gctgacccgc
    15301 gttggcatgg tgcattttgc ccatcagcgc gtttccaccc tctccggcgg ccagcagcaa
    15361 cgtgtggcga ttgcccgtgc gctgatgcag caggcgaaag tgattctggc cgatgaaccc
    15421 atcgcctcgc tggacccgga atcagcgcgc atcgtgatgg acaccctgcg cgacatcaac
    15481 cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg tggattacgc cctgcgctac
    15541 tgcgaacgca tcgtcgccct gcgtcagggg cacgtcttct acgacggcag cagccaactg
    15601 tttgataacg aacgttttga ccatctctac cgcagcatta accgcatcga agagaacgcg
    15661 aaagctgcct gatatcccca tcattgagga aaacgaatga acgctaagat aattgcctcg
    15721 ctggccttca ccagcatgtt cagcctcagc accctgttaa ccccggcaca cgccgaagag
    15781 caggaaaagg cgctgaattt cggcattatt tcaacggaat cacagcaaaa cctgaaaccg
    15841 caatggacgc cgtttttaca ggatatggag aagaaactgg gcgtgaaggt caacgccttc
    15901 tttgccccgg actacgcggg cattatccag gggatgcgct tcaataaagt ggatattgcc
    15961 tggtacggca acctgtcggc gatggaagcg gtggatcgcg ccaatggcca ggtcttcgcc
    16021 cagacggtcg cggcggatgg atcgtcgggt tactggagcg tgttgatcgt caacaaagac
    16081 agcccgatca acaacctgaa cgatctgctg gcgaagcgga aagatctcac cttcggcaat
    16141 ggcgatccta actccacctc tggcttcctc gtccccggtt actacgtctt cgctaaaaac
    16201 aatatctccg ccagcgactt caagcgcacc gtcaacgccg ggcatgaaac caacgcgctg
    16261 gccgtcgcca acaagcaggt ggatgttgcc accaacaaca ccgaaaacct cgacaagctg
    16321 aaaacctccg cgccggagaa gcttaaagag ctgaaggtga tctggaaatc gccgctgatc
    16381 ctaggcgatc cgatcgtctg gcgcaagaat ctgtccgaaa ctaccaaaga caagatctac
    16441 gacttcttta tgaactacgg aaaaacgccg gaagagaaag cggtgctgga acgcctaggc
    16501 tgggcgccgt tccgcgcttc cagcgacctg caactggtgc cgattcgcca gctcgcactg
    16561 tttaaagaga tacaaagcgt gaaaggcaat aaagggctga atgagcagga caagctggcg
    16621 aaaaccaccg agattcaggc gcagctggat gacctggacc gcctgaacaa cgcgttaagc
    16681 gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag gtcggataag acgccccggc
    16741 gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg tgcaagcacc ttatccggcc
    16801 tacagaccgg agccaaacat gcaaaccatc accatcgccc cacccaagcg tagctggttc
    16861 tcgcttctga gctgggccgt tgttctcgcc gtgctggtcg tctcgtggca gggcgcggaa
    16921 atggccccgc ttacgctgat taaagacggc ggcaacatgg caaccttcgc tgccgacttc
    16981 ttcccacccg atttcagcca gtggcaggat tacctcaccg aaatggccgt cacgctgcaa
    17041 atcgccgtct ggggcaccgc gctggcagtg gttctctcca tccccttcgg tctgatgagc
    17101 gccgaaaact tggtgccgtg gtgggtttac cagcccgttc gccgcctgat ggacgcctgc
    17161 cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg cctcggcccg
    17221 ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa gctgctttcc
    17281 gaagcggagg aagcgatcga acccggcccg gtggaaggca ttcgcgccac cggcgccaac
    17341 aaactcgaag agatccttta cggcgtgctg ccgcaggtga tgccgctgct gatctcctac
    17401 tccctctatc gcttcgaatc caacgtccgc tcggcgaccg tcgtcggcat ggtcggcgcg
    17461 ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca gcaaacctgc
    17521 gccctgatgg tgcttatcat cgtcacggtc agcctgctgg atttcctctc tcaacggttg
    17581 cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct acacatccga
    17641 ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag cttcgtcaac
    17701 actaccgctg cggcgactat cttcccgccg agcagcaact ggcggcgcgc tttgaggtga
    17761 accgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg gtacagcgcc
    17821 gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc aacgcccagg
    17881 cgcgttttag ccagaacctg ctggaccagg gcagccatcc caccagcgaa aaactgctgt
    17941 cggtattgcg ccccgcgtcc ggtcacgttg ctgacgcgct ggggattacc gagggcgaga
    18001 acgttatcca tttgcgcacc ctgcgccggg tcaacggcgt cgcgctctgt ttaatcgacc
    18061 actactttgc ggacctcacc ctttggccga cgctgcaacg cttcgacagc ggttcgctgc
    18121 acgattttct gcgcgagcaa accggaattg cgctgcgccg cagccagacg cggatcagcg
    18181 cccgccgcgc ccaggccaaa gagtgccagc gccttgaaat cccgaatatg tcgccgctgc
    18241 tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag tactccgtca
    18301 gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac gcagataccg
    18361 cgacccgcca gcactggatg tccgtactgg cgcacagcca accggctgaa ctggcagccc
    18421 gcctgaacgc gctgaacatc accgccgact atgaagtgat ccgcaccgcc gaaaccggcc
    18481 tggtacagat tcaggcgcga atgggcggca ccggcgaacg cttttttgcc ggcgacgcca
    18541 cgctgacccg cgccgccgta cgcctgactg acggcaccct cggctacagc tgggtgctgg
    18601 ggcgtgataa acagcacacc gaacgctgcg cgctgattga tgcgctgatg cagcaatctc
    18661 gccactttca aaacttatca gaaaccctta ttgccccgct ggacgctgac cgtatggcac
    18721 gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt acgatgattc
    18781 gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg atgcccagca
    18841 cagttttcgt cgcctgttaa aggccataag cgagccgggc gtgattgtcg ccctgcatca
    18901 gctcaagcgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc tgacgctggc
    18961 tgataacgac acgccggtgt ggcttgccgc gccattgagt aacgatatcg tcaatcagag
    19021 cctgcgtttt cataccaacg cgccgctggt cagccagccg gaacaggcga ccttcgcggt
    19081 gacggatgac ggaatttcca gcaaacagct caacgccctt tccaccggca ccgccgttgc
    19141 gccggaagcg ggcgcgacgc tgattttaca ggtcgccagc ctgagcggcg ggcgcatgtt
    19201 gcgtctcacc ggcgcgggta ttgccgaaga acgaatgatc gctccgcagc tgccggagtg
    19261 cattctgcac gaactcaccg agcgcccgca cccgttcccg ctcggcatcg acctgatcct
    19321 gacctgcggc gaacgcctgc tggctattcc gcgaaccact catgtggagg tgtgctgatg
    19381 tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct gcaagagagc
    19441 cgacgccggg gcgataccga tttgcccgaa ctgagcgtcg cccaaattga acagcagctt
    19501 aatctcgcgg tggatcgcgt gatgaccgaa ggcggcgttg ccgaccgcga actggcggcg
    19561 ctggcgctga aacaggccag cggcgataac gttgaagcaa ttttcctgct gcgcgcctac
    19621 cgcaccacgc tggcgaaact ggcggtaagc gagccgctcg acaccaccga gatgcgtctc
    19681 gaacggcgta tttcggcggt ttataaagac attcccggcg gccagctgct tggcccaacc
    19741 tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggtga agcgccgacg
    19801 ctgaccaccg ccgacagcga acaacagccg tcgccgcacg ttttcagcct gctggcgcgt
    19861 caggggctgg cgaagtttga agaagatagc ggcgcacagc cggatgacat cacccgcacg
    19921 ccgccggttt acccctgctc acgctcctcc cgcctgcaac aattaatgcg cggggacgaa
    19981 ggctatttgc tggcgctggc ctactccacc cagcgtggtt acggacgcaa tcacccgttc
    20041 gcgggcgaga tccgcagtgg ttacatcgac gtgtcgattg tgccggaaga gctgggattt
    20101 gcggtaaacg tcggcgaact gctgatgacc gagtgtgaaa tggtcaacgg ttttatcgac
    20161 ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtattcgg catgagcgag
    20221 cgcaaagcga tggcaatggc gctggttgac cgtgcgttgc aagccccgga atacggcgag
    20281 cacgcgacag gcccggcgca ggatgaagag ttcgtgctgg cgcatgccga caacgtcgaa
    20341 gccgcaggct ttgtctcgca cctcaaactc ccccactacg tcgatttcca agccgaactg
    20401 gagctactca aacgtctgca acaggagcag aaccatggct aatctgagcg gctacaactt
    20461 tgcctacctc gacgagcaga ccaaacgcat gatccgccgc gccatcttaa aagcggtggc
    20521 tatccccggt tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccct acggctgggg
    20581 aaccggcggc attcagctca ccgccagcgt gattggcgaa agcgacgtgt tgaaggtgat
    20641 tgaccagggc gcagacgaca ccactaacgc cgtgtcgatt cgcaacttct tcaagcgcgt
    20701 gaccggggtg aacaccactg aacgtacgga cgatgcgacg cttatccaga cgcgccatcg
    20761 catccccgaa acgccgctga ccgaagatca gatagttatt ttccaggtgc cgatccccga
    20821 accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc actatgcacg cgctggagga
    20881 gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc atatcgccac
    20941 cacctatgct tatccggtga aggtgaacgg gcgctacgtg atggacccgt cgccgatccc
    21001 gaaattcgat aacccgaaaa tggacatgat gcccgccctg cagctgttcg gcgcagggcg
    21061 cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagtctcg atttcgacga
    21121 tcacccgttc accgttcagc agtgggatga gccatgcgcc atctgcggat cgacccacag
    21181 ctatctcgac gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct gctccgatac
    21241 cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt actttccgtc
    21301 aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc ttttgattta
    21361 tggccggggg aagtgctggg catcgtcggg gaatccggct ccgggaagac cacgctgctg
    21421 aaatcaatct ccgcgcgcct gacgccgcag cagggggaga ttcgctacga gaatcgttcg
    21481 ttgtatgcga tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg gggcgtggtg
    21541 catcagcatc cactcgacgg cctgcgtcgc caggtctcgg caggcggcaa tatcggcgag
    21601 cggctgatgg cgaccggggc ccgtcattac ggcgacatcc gcgccaccgc gcagaagtgg
    21661 ctggaagagg tggagattcc cgccaaccgg atcgacgacc tgccgaccac cttttccggc
    21721 ggcatgcagc aacgtttgca gattgcccgc aacctggtga cgcatccgaa gctggtgttt
    21781 atggacgagc caaccggtgg gctggatgtg tcggtgcagg cacgcctgct cgatctactg
    21841 cgcggcctgg tggtggagct gaacctggcg gtggtgattg tcacccatga tttaggcgtc
    21901 gcccgcctgc tagcggaccg tttgctggtg atgaagcagg ggcaagtagt ggagagtggg
    21961 ttaaccgacc gcgtgctcga cgacccgcac catccgtata cccagctgct ggtgtcatcg
    22021 gttttgcaga attgagccgg tgccggatgc ggcgtaaacg ccttatctgg cctacaaatg
    22081 cgctccccgt aggtcggata agacgcatca gcgtcgcatc cgacacccga accacgaggc
    22141 gaaaaatgat taacgtacaa aacgtcagta aaaccttcat cctgcaccag caaaacggcg
    22201 tgcgcctgcc cgtcctcaac cgcgcctcgc tcaccgtcaa cgcgggcgaa tgcgtggtgc
    22261 tccacggcca ctccggcagc ggcaaatcaa ccctgctgcg ctcgctgtac gccaactatc
    22321 tgcccgatga agggcaaatc cagatcaaac acggtgacga gtgggtagac ctggtcaccg
    22381 cgccagcgcg caaagtggtg gagatccgca aaaccaccgt cggctgggtg agccagtttc
    22441 tgcgcgtcat cccgcgtatt tcagcactgg aagtggtgat gcagccgctg ctcgataccg
    22501 gcgtaccgcg tgaagcctgc gccgctaaag ccgcgcgtct gctcacccgc ctgaacgtgc
    22561 cggaacgcct gtggcacctg gcgccatcaa ccttctccgg cggcgaacag cagcgcgtca
    22621 acatcgcccg cggctttatc gtcgactacc cgattctgct gctcgacgaa cctaccgcct
    22681 cgctggacgc caaaaacagc gccgcggtgg tggaactgat ccgcgaagcc aaagctcgtg
    22741 gcgcagccat tgtcggcatc ttccatgacg aagccgtacg taatgacgtt gccgaccgcc
    22801 tgcacccaat ggaagcctct tcatgattat caataacgtc aaattggtgc tggaaaacga
    22861 agtggtacac ggttcactgg aggtgcagga cggcgaaatc cgcgcctttg ccgaaagcca
    22921 gagccgcctg ccggaggcga tggacggcga aggcggctgg ctgctgccgg ggctgattga
    22981 gctgcatacc gataatctgg ataaattctt caccccgcgc ccgaaagtcg actggcccgc
    23041 ccattcggcg atgagcagcc acgacgcgct gatggtggca agcggcatca ccaccgtact
    23101 ggacgccgtg gcgattggtg atgtgcgcga cggcggcgat cggttggaga atctggagaa
    23161 gatgatcaac gccatcgaag agacgcagaa acgcggcgtc aaccgcgccg agcaccgtct
    23221 gctcctgcgc tgcgaactgc cgcatcacac cacgctgccg ctgtttgaaa aactggtgca
    23281 gcgcgagccg gtgacgctgg tgtcgctgat ggaccactcg ccgggccagc gccagttcgc
    23341 caaccgcgag aagtatcgcg aatattatca gggcaaatac tcgctcaccg atgcgcagat
    23401 gcagcagtac gaagaagagc aactggcgct tgccgcacgc tggtcgcagc cgaatcgcga
    23461 atccatcgcc gccctgtgcc gcgcgcgaca aattgcgctc gctagccatg atgacgccac
    23521 ccacgcccac gttgccgaat cccacctgct tggcagcgtg atcgccgaat ttcccaccac
    23581 gtttgaagcg gcggaagcct cgcgcaagca tggcatgaac gtactgatgg gcgcgccgaa
    23641 tatcgtgcgc agcggttcgc actccggcaa cgtggcggcc agtgaactgg cgcagcttgg
    23701 cctgctggat atcctctctt ccgactacta ccccaccagc ctgctggatg cggcatttcg
    23761 cgtcgccgat gaccagagca accgctttac gctgccgcag gcggtgaagt tagtgaccca
    23821 aaatccggcg caggcgctta atcttcagga tcgcggggtg attggcgagg gcaaacgcgc
    23881 ggacctggtg ctggcacatc gcaaggacaa tcatattcat atcgaccacg tctggcgtca
    23941 gggtaaaagg gtgttctgat gatgggaaaa ctgatttggt taatggggcc gtccggctcc
    24001 gggaaagaca gcctgctggc ggaaatccgc ctgcgggaac aaactcagtt actggtggcg
    24061 catcgctaca ttacgcgcga cgccagcgcc ggaagtgaaa accatatcgc cctgagcgag
    24121 caggagtttt ttacccgcgc ggggcaaaat ctgttggcct taagctggca cgctaacagt
    24181 ctgtattatg gcgtcggcgt tgagattgat ctctggctgc acgccggatt cgacgtgctg
    24241 gtcaacggct cacgcgccca tctgccgcag gcgctggcgc gctatcaatc ggcgctgctg
    24301 cccatttgct tactggtttc gccggagatc ctgcgccagc gcctcgaaaa ccgtggtcgt
    24361 gaaaatgcca gtgaaattaa cgcccgacta gcacgcgctg cccgctatac tcctcaggat
    24421 tgtcttacgc tcaataatga cggcagcctg cgccagtcgg tcgacaagct gctgacgctg
    24481 attcatcaga aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta cgcagtatga
    24541 caccgacgca gtttacgcgc tgatttgtga gctaaaacag gcggagtttg accaccatgc
    24601 gtttcgcgtg ggctttaacg ccaacctgcg cgacccaaac atgcgctacc atctggcgct
    24661 gctggatggc gaagttgtca gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt
    24721 caactggatc ggcgaaattc aggaactggt ggtgatgccg caggcgcgcg gtctgaacgt
    24781 cggcagtaaa ttactggcat gggcagaaga agaagcccgc caggccgggg ccgaaatgac
    24841 cgcgctttcg accaacgtga aacgccacga cgcgcaccgt ttctatctgc gagaaggcta
    24901 cgagcagagt cacttccgct tcaccaaggt gctgtaacat gagcctgacc ctcacgctca
    24961 ccggcactgg cggcgcacag ggcgttccgg
//
LOCUS       DAJTYF010000048        24816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTYF010000048 DAJTYF010000000
VERSION     DAJTYF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.048"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            72..353
                     /locus_tag="OO590_002109"
     CDS             72..353
                     /locus_tag="OO590_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR6637476.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            319..1396
                     /locus_tag="OO590_002110"
                     /pseudo
     CDS             319..1396
                     /locus_tag="OO590_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1789..2130)
                     /locus_tag="OO590_002111"
     CDS             complement(1789..2130)
                     /locus_tag="OO590_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6637477.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2143..3015)
                     /gene="copD"
                     /locus_tag="OO590_002112"
                     /pseudo
     CDS             complement(2143..3015)
                     /gene="copD"
                     /locus_tag="OO590_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="OO590_002113"
     CDS             complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="OO590_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6637478.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3532..3762
                     /gene="holE"
                     /locus_tag="OO590_002114"
     CDS             3532..3762
                     /gene="holE"
                     /locus_tag="OO590_002114"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6637479.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3864..4520
                     /locus_tag="OO590_002115"
     CDS             3864..4520
                     /locus_tag="OO590_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR6637480.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4544..5206
                     /gene="exoX"
                     /locus_tag="OO590_002116"
     CDS             4544..5206
                     /gene="exoX"
                     /locus_tag="OO590_002116"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6637481.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="OO590_002117"
     CDS             complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="OO590_002117"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6637482.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7472..8131)
                     /locus_tag="OO590_002118"
     CDS             complement(7472..8131)
                     /locus_tag="OO590_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6637483.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8458..8814)
                     /locus_tag="OO590_002119"
     CDS             complement(8458..8814)
                     /locus_tag="OO590_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6637484.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="OO590_002120"
                     /pseudo
     CDS             complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="OO590_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9305..10483
                     /gene="purT"
                     /locus_tag="OO590_002121"
     CDS             9305..10483
                     /gene="purT"
                     /locus_tag="OO590_002121"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6637485.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10538..11179)
                     /gene="kdgA"
                     /locus_tag="OO590_002122"
                     /gene_synonym="eda"
     CDS             complement(10538..11179)
                     /gene="kdgA"
                     /locus_tag="OO590_002122"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6637486.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11216..13027)
                     /gene="edd"
                     /locus_tag="OO590_002123"
     CDS             complement(11216..13027)
                     /gene="edd"
                     /locus_tag="OO590_002123"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6637487.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="OO590_002124"
     CDS             complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="OO590_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6637488.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15075..15944
                     /gene="hexR"
                     /locus_tag="OO590_002125"
                     /gene_synonym="yebK"
     CDS             15075..15944
                     /gene="hexR"
                     /locus_tag="OO590_002125"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6637489.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16072..17514
                     /gene="pyk"
                     /locus_tag="OO590_002126"
     CDS             16072..17514
                     /gene="pyk"
                     /locus_tag="OO590_002126"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6637490.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="OO590_002127"
                     /gene_synonym="msbB"
     CDS             complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="OO590_002127"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6637491.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="OO590_002128"
     CDS             complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="OO590_002128"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6637492.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLLFD"
     gene            complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="OO590_002129"
     CDS             complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="OO590_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6637493.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21085..21840
                     /gene="znuC"
                     /locus_tag="OO590_002130"
     CDS             21085..21840
                     /gene="znuC"
                     /locus_tag="OO590_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6637494.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21837..22622
                     /gene="znuB"
                     /locus_tag="OO590_002131"
     CDS             21837..22622
                     /gene="znuB"
                     /locus_tag="OO590_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6637495.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="OO590_002132"
     CDS             complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="OO590_002132"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6637496.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="OO590_002133"
     CDS             complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="OO590_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6637497.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(24538..24603)
                     /gene="yobI"
                     /locus_tag="OO590_002134"
     CDS             complement(24538..24603)
                     /gene="yobI"
                     /locus_tag="OO590_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6637498.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24674..>24816
                     /locus_tag="OO590_002135"
     CDS             24674..>24816
                     /locus_tag="OO590_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637499.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGE"
BASE COUNT         6161 a         6277 c         6096 g         6282 t
ORIGIN      
        1 ttattaaaaa tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtga
       61 gaggtatggc aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata
      121 acccaccatc aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac
      181 ctgaactaca cgggaggagt tacagggtgg ttccggaggc tttctatatc aacccaaata
      241 aggttgatac cgatataaca caccatcagc ctaatgggcg acaagggaga gacagtccgt
      301 taatggagaa gttaaaacat gcagcggaaa aaatacgatc ccaatttgcc tagaaactta
      361 acatatcgaa ggagagacaa agcatattac tggcgcaacc ctctgacgaa agaagaattt
      421 acactaggta aaatttcaag aagagatgca gtagcgcagg caattgaagc aaatcattat
      481 atatacaaaa actactctcc tgctgcccta attgaaaagc ttaaaggggt cgactcattt
      541 actatggcag actggattga acgttacaaa acgattctta taaggagaaa agcgtccaga
      601 aatacttata aaactcgggt aaatcaactg gtgacaataa aagaaaaatt aggaggggtt
      661 ttactgacag aaataaccac tcgccatatc gccgagtttc ttgatttgtg gattgaagga
      721 gggaaaaaca cgatggcagg atcaatgcgt tctgtgttat ctgatatgtt ccgcgaagcc
      781 attgttgaag gacgtatatc tcaaaatcca gtaacgccaa caagagcacc gaaaatagta
      841 gttacaagag aacggctgaa actaaagaca tacaactgca tcagggaggc agcagatcaa
      901 cttccggcat ggttcccatt agctatggac ttagcccttg taacaggaca acgtcgcgaa
      961 gacataacga atatgcggtt cagtgatatt tatgatgatc gtctccacat caggcaaatt
     1021 aagacaggaa tgatgattgc tatccccctg tcactcagcc ttcctgtcgc tggtttacga
     1081 cttggtgcag tagttgaaca gtgccgcatg gtaagtaggg ggggattatc taatcagtgc
     1141 cgggattaga aaaaacagcc ctgacggcag cattcacccg gacggcctga caaagaaatt
     1201 tgtcgcagcc agaaaattaa caggtatcct gttcagtgaa aacccaccaa cttttcacga
     1261 aatcagaagc ctggctggac gattgtacaa agaaatatgt ggagaagaat ttgctcagcg
     1321 tctacttggc cacacatcgg agaagacaac aaaaatgtat cttgatgaga gagaaaaaac
     1381 gtacttactg ctctgatttt aacgtaaatg gattgttaaa tgtattttgg ttgtgatata
     1441 accaaaaaag accggaatac agaaattcga gtaaatttcg gggaatttcg gggagacgtt
     1501 tgcaactaat tgattttaaa tacaattaaa aaaagaccga atacgattcc tgtattcggt
     1561 ccagggaaat ggctcttggg agagagccgt gcgctaaaag ttggcattaa tgcaggctta
     1621 gttgccttgc cctttaagaa tagatgacga cgccaggttt tccagtttgc gtgcaaaatg
     1681 gtcaataaaa agcgcggtgg tcatcagctt aaatgttaaa aaccgcccgt tctggtgaaa
     1741 gaactgaggc ggttttttta ttggaaatca aaaggctatt ttaggtaatt aacagagttt
     1801 ttcagctcgt tctataaacg gtgccagact cattttttcg ccgggattgt taggatcatc
     1861 aatctgaatc accgaaatgg gttgggcatt ggtcttccca ctggcaactt ccttttgtgc
     1921 gatatcgttt aaaggatact gcacgagggt acttgggttg atgacataca aagcattacc
     1981 cggtcggcaa gtcagcatta cctcttcgcg attaaacgcc catttgtctt tacccacttc
     2041 aaaacgactc acggttatca cctgtggagc cgccagcgcc gctgcagaac tggtgagtaa
     2101 cagaaacgcc agaatacttt ttttcatcat tttcgtaatc catcaaaaag gttcgagagt
     2161 cgcaagcagg ctgatgatca ccagcactac cgcacctatc gcccattcga gtttagtcat
     2221 ccacacaaac cagggtgccg cacgatcttc atcctgcctc atgcgcggta caagaacata
     2281 acgattagcc agcgcaatga ccaccataat catcaccagg atggctttga gcaataagag
     2341 ttggccctag taagtcgtaa gcgtgggggg aaatccggta atcaacaatg cattaagcac
     2401 gccgcttgcc agtacgccga tcaccgcaaa atgcccgcac caggaaaaac gcatcagcgc
     2461 ctgaatagcc tggtgtcgcc aacggccttt gatgagttgc atacaccaga gcaccggcag
     2521 taatccccca aaccacgccg ccgcacaaat caggtgaatc gcgtgattag tctgatggat
     2581 tttcgctgtt accccttcat tcagcgtcgc atgccccact cccgccagca gaataaattg
     2641 cgcggtagtg agcataaaca acaaacgtgg catattccgc ggttgcataa gggcgacgat
     2701 caacgttacg agggcgagaa caatttgcca taaccagacg ccaccaaact gcgtttgcaa
     2761 aacagcctgc caaatgtttg gcgaaaatac atctgtccat cccgttccca tcaacccgcc
     2821 ctgaacggcg agcattgcag tggcactgat caaactccag acggcagcgt gctgttgtaa
     2881 acgcagagaa cgcttcgtca gcaagcgacg aatcattaag ggggccagcc aggcaccata
     2941 cattgcaaag ccaaagacca gcatcaacga ggtgaaatgg ataaatcgca gcgcgatcca
     3001 ggtaaacgcc agcatgattt atttcacgct aaaggtgtag tgtcctttcg ttttatgccc
     3061 gtccaccgaa acaacatgcc agtcaacggt gtaggttccg ggtttcagcg aatcggccag
     3121 tggaacaatc agttgctttt ggtcctgctc attccgcttc gctggtaatg ttttaatatt
     3181 ttcgtttttt ggccccgtga tttttgcacc actgaatcct gtttcaacgc cttccgagaa
     3241 gtttaaagtg attgcctgcg gggcagctgt cacttgcgcg tttgccgcag gatactgatg
     3301 cgttaaatgc gcatgtgccc agacggaagg tgtcaccaac gaagtggtca ggattgccag
     3361 cgcgtagcga agggagcgtg cagttgaagc catattatct attccttttt gtaataactt
     3421 ttttacagag cataaccttg tctaatgtct gagtcgagga tcatcaattc cggcttgcca
     3481 tccaggctca ctcttagtaa cttttgcccg cgaatgatga ggagattaag aatgctgaag
     3541 aatctggcta aactggatca aacagaaatg gataaagtga atgtcgattt ggcggcggcc
     3601 ggggtggctt ttaaagaacg ctacaatatg ccggtgatcg ctgaagcggt tgaacgtgaa
     3661 cagccagaac atttgcgcag ctggtttcgc gagcggctta ttgcccaccg tttggcttcg
     3721 gtcaatctgt cacgtttacc ttacgagccc aaacttaaat aaaacttata cagagttaca
     3781 ctttcttaca taacgcctgc taaattatga gtattttcta aaccgccccc ataatttgca
     3841 gtcattttga aaaggaagtc attatgtcgt tctggaaagt tgcagcagcg caatatgagc
     3901 ctcgtaaaac ctcactcacc gaacaggtag ctcatcatct ggagtttgtg agggccgccg
     3961 ccaggcaaca atgtcagctg ttagtttttc catcgctttc tttactgggg tgtgattatt
     4021 cgcgacgtgc ccttcctgcc ccacccgatc tttcactgtt agacccgctt tgttatgccg
     4081 caacgacctg gcgaatgacc atcattgccg gccttcccgt tgaatataac gatcgcttta
     4141 ttcgtggaat tgcggtgttc gcaccgtggc gaaaaacgcc tgggaactac catcagagtc
     4201 atggtgcatg tctgggccgt cgttccagaa ccatcacggt ggtcgatgaa caaccgcaag
     4261 gtatggatat ggacccgacc tgttcactgt ttacgacagg gcaatgtctg ggagaacccg
     4321 acctgctggc gtcggcccgt cgcttacaat ttttttcaca tcagtactcg attgccgtgc
     4381 tgatggcaaa tgcccgtggt aacagtgcac tatgggatga atatggtcgt ctcatcgttc
     4441 gcgccgatcg tggttcatta ttgttagtcg gtcagcgttc atcacagggt tggcaaggcg
     4501 atatcattcc attacgctag gctttttcgg cctggagcat gccatgttgc gcattatcga
     4561 tacagaaacc tgcggtttgc agggagggat cgttgagatt gcctctgttg atgtcattga
     4621 cggaaaaatc gtcaacccca tgagcctcct ggtgcgcccc gatcgtccta ttagtccaca
     4681 agcgatggcg attcatcgca tcaccgaagc catggtcgcc gataaaccgt ggattgaaga
     4741 tgtgatccca cactattacg gtagtgaatg gtatgtcgcg cacaacgcca gctttgaccg
     4801 ccgcgtactg cctgagatgc ccggtgagtg gatttgcact atgaaactgg cccgtcgttt
     4861 gtggccaggg ctcaagtaca gcaatatggc gttatataaa acacgcaagc tcaatgtaca
     4921 gacaccgccg ggcctgcatc atcaccgcgc gttgtatgac tgttatatca ccgccgcgtt
     4981 gcttatcgat attatgaaca cctccggctg gacggcagaa cagatggccg atatcaccgg
     5041 acgtccgtcg ttgatgacga ccttcacctt tggcaaatac cgtggcaaag cggtttccga
     5101 cgttgccgaa cgcgatccgg gctatctgcg ctggttattt aataacctgg acagcatgag
     5161 cccggagctg cgtttaacac tgaaacatta tctggaaaat acttaatccg caggcgtagt
     5221 gggtaatgtt ccctgcgcca gcgcgaccag aaaagcatat tccatcgcta cgccttcgta
     5281 cgatttaaag cggccagatt taccgccatg gcctgagtcc atgtcggtac agagcaataa
     5341 aagatggtca tcggttttca gctcgcgcaa tttagcgacc cattttgccg gttcccaata
     5401 ttgcacctga gaatcgtgta aaccggtcgt taccagtaaa tgcggataag cctgtgcggt
     5461 gacgttgtca tacgggctgt agcttttcat gtactcgtaa tattgcggat cctgcggatt
     5521 tccccactct tcaaactcac cagtggtaag aggaattgat tcatcaagca tcgttgttac
     5581 aacatcaaca aacggtacct gggcgataac gccgtggaat aattccgggc gttgattaat
     5641 tgcaacgccc atcaacatgc cccccgcact tccgcccatc gcataacaga gcgaaggaga
     5701 gccatagccc agttttaaca atgcatcgca ggcatcaaga taatcattaa acgtattttt
     5761 cttcttcaga aatttaccgt cttcgtacca ttgttgcccc agctcaccac cgccgcgaac
     5821 atggacaatg gcgtagacaa agccacgatc taacaaactc aagcggctaa aactgaaatc
     5881 ggcatcaata cttgcgccgt aagaaccata gccatacacc agcaacgggt tgtgtccttt
     5941 gcgaaaatgt ttgcgatgat agaccagcga aaccggaact tcgacgccat cacgggcgac
     6001 tatccacagg tgttcactgc ggtaattcgc cgcatcaaaa ccgggcactt ctgtttgttt
     6061 taatacacga cgctcaccgg tatccatatc cagttcaaac aaagtgtctg gtgtggtcat
     6121 ggaagaataa ccataacgca atcgcgcggt ttcaggttct ggattgtagg caatccaggt
     6181 cacataggcc ggatcatcaa aggcaatgcc aatgacttcc cgggtcttgc ggttaatttg
     6241 gcgcaaactg gttaacccgc gctgacgctc ttcaaccacc agccagtcgg taaacagcgt
     6301 aaacccttcc agcatgatgt tttcacgtgg cggaattaac tcttcccact gttgctcatc
     6361 acgcatacgg gtacggtata agccaaagtt tttgccgtgg cgattggaac gcagataaaa
     6421 acgatgctgg tagtgatcaa ggctgtattc gtgatctttg cggcgcggca gaaaaacaaa
     6481 cggctcggca tcggccattt ccgcgtccag caggcgaact tcactggtgg tggcgctggc
     6541 cagatgaatg acgacatagt gcttcgacgt cgttttatgc aggctgacat aataggtatc
     6601 gtctttttct tcgtagatca gtgtatcttg cgatgctggc gtaccgatgg cgtgacgcca
     6661 gacctgataa ggcagcagcg tcaccggatg cttgcgaaca tagtagaaag tccaggagtc
     6721 atttgcccag acaaagctgg gttcaacgtt atccagcagt tccgggtacc agttacctgt
     6781 ttccagatta cgaaaacgaa tgccgtactg gcgtcgggaa agaaaatctt ctgccagcgc
     6841 cataatggtg ttatcgggcg taatcgccat tccgcccatc gaataaaact cactatgagc
     6901 tgcgcgcttg ttggcatcga gcaatgtttc ccactcatcc cactcttcgc tgaatgccga
     6961 ttgacgctgg tagatagcat attcacagcc tggttcataa atatgccgat agcggtagcc
     7021 attttttatg tagggcgcag aaacttctct ttgcggaatg cggtcgatga tttcctttaa
     7081 gatgcgatcc tgcaaggctt gttgtgaggc catcacccga tgaccgtaac tattttcttg
     7141 ttgcaggtag tccaggactt ctggctgaga acgcgtatcg tcccgcagcc agtagtaatt
     7201 atcgatgcgc gtatcgccat gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag
     7261 catgttattg ttctttctgg ttgaaacatc ttataagggt ggcaaaactc accggggatg
     7321 caagcgaaac agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg
     7381 ttatgcagac agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa
     7441 tagtcaactt caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt
     7501 cgagatcgcg ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg
     7561 cattcaggta actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat
     7621 agatttccag cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg
     7681 gttgttcgat tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca
     7741 attgctggtc gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa
     7801 agaccagcgc aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa
     7861 actgcggttc gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg
     7921 tacccgctac cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt
     7981 tacgtgctga tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctggcacta
     8041 acagtttgcc caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca
     8101 gggattgcag ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc
     8161 ctgtagcata gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata
     8221 agcggggcgc taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat
     8281 caggtgataa gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg
     8341 ctgattgccc gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg
     8401 gacggacaag gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta
     8461 acgccgctga tattccgcca ttcccgcttt gcagtccacg ctgacctggt aatgaatatc
     8521 ggcactttta ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc
     8581 ctgcaaactg acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca
     8641 gcgcgccacg cgattttgtt gataatcacg ttttacgctc gcggcaattc cggtagcatc
     8701 cagaccttca cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac
     8761 tgatgcgcag gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt
     8821 ctccatagca caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct
     8881 tcaggccgcg tgtgaggcat cttcctcttc ggattcagca ccgacgacca gtggggacgg
     8941 taatttgccg gttttcagaa tggtgctcag catatctttt tgttctgcca gccatagcga
     9001 aagggcttca cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag
     9061 cagatccgcc gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga
     9121 cattttctct tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct
     9181 ctcataataa ctgtgatttt atacagtata tttcttttcg gttgagaaat caacatcagc
     9241 aataaagaca cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt
     9301 cgttatgacg ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg
     9361 ctccggtgaa ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat
     9421 tgccgtcgat cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat
     9481 taatatgctt gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat
     9541 cgtgccggag atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact
     9601 gaatgttgtc ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg
     9661 cctggcggca gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag
     9721 ccttttccgc gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag
     9781 ctcttccggc aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga
     9841 atacgctcag caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa
     9901 gtttgacttc gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc
     9961 accagtaggt catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat
    10021 gagcccgctt gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg
    10081 cggttatggt ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga
    10141 ggtctcccct cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga
    10201 gtttgccctg catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg
    10261 tcctgcagct tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa
    10321 tgtgcagaat gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga
    10381 tggcagccgt cgtctggggg tggtactggc tacttcagag agtgttgttg acgccattga
    10441 acgcgcgaag cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaacgct
    10501 acaaaaatgc ccgatcctag atcgggcatt ttgactttta cagcttagcg ccttctacag
    10561 cttcacgcgc cagcttagta atgcggtcgt aatcgcccgc ttccagcgca tctgccggaa
    10621 ccagccagga accaccgatg cacagcacgc ttttcagtgc caggtagtca cggtagttag
    10681 ccggagaaat accacccgtc gggcagaaac ggacctggga gaacggaccc gcgatcgcct
    10741 gcagggcttt cacgccgccg ttagcttcag ccgggaagaa tttgaactct ttcaaaccgt
    10801 agtccatacc cagcatcagt tcggaaacag tgctgatccc cggaatcaga ggaatagtcc
    10861 cttcggtagc agctttcagc agcggctcgg tcagacccgg gctaattgcg aactgtgcac
    10921 ccgcttcagt gacttctgcc agctgctgtg gattcagcac cgtaccggca cccacaatcg
    10981 cttcaggcac ttctttggcg atagcacgga tagcgtcaac tgcacactcg gtacgcagag
    11041 tcacttccag aacgcgcacc ccaccagcaa ccaacgcttt tgccatcggc accgcgtgtt
    11101 ccagtttttt taccacgata accggtacaa ccgggccggt ggtcaggatt gattctgcac
    11161 ttgttttcca gtttttcatc agagttttct ctcgcctgat tacaaatttg tcgtcttaaa
    11221 aagtgataca ggttgcgccc tgttcggcac cggacagttt ttcacgcaag gcgctgaata
    11281 attcacgtcc tgttctcacg cgtgacgcgc tcaggtcagg aatgtgcggt tcgcgagcag
    11341 ccagttccgc ttcgtctacc agcagtgtca gttcgcctgt ctgtccattc acacgaatga
    11401 tgtccccgtc gcgcactttt gccagcagcc cgccatcgta ggcttctggt gttacgtgga
    11461 tagctgacgg cactttacct gaagcgccgg agagtcgtcc atcggtaact aacgcaattt
    11521 tgaaacaccg gtccaataat acaccaagtg gcggcatgag tttatgtaat tctggcattc
    11581 cgttcgcttt tggcccctga tgacggacaa caacgacaca atcgcggtcc agcaaacccg
    11641 cttcaaaggc cggcataacg tcatgctggc tttcaaaaac aaccgctggc gcttcaatca
    11701 cctggttctc aaccggcacg gcagaggttt tcataaccgc acggcccagg ttaccgctta
    11761 acacttttgt cccaccatga tgagagaaag gttgttcgaa ggaagcgatc acattgctgt
    11821 cgagtgattt ttccgcccct tcccgccagt ccagttcacc attattcagc catggttcaa
    11881 gggtataacg aaacagacca aagcctgcca ccgtattgac atcttcatgc agcaggcctg
    11941 ctttgagcag ttcacgcacc agaaccggta cgccacctgc cgcctggaag tggttaatat
    12001 cggccggacc gttcgggtag agacgtgcca tcagcggtac aacatcagaa aggtcagaga
    12061 agtcatccca gttaatctga ataccggccg cgcgcgccat cgccaccagg tgcatggtgt
    12121 ggttagtgga accaccggtc gccaacagtg caacgatacc gttcaccacc actttctcat
    12181 cgatcatctt accgatcggc atccattcat taccattacc ggtcatgcgt gtaacctgac
    12241 gcgcagcggc agcggtcaaa gcatcgcgca gcggagaatc cggatgaaca aaagaagagc
    12301 ctggcaactg catccccata aattccacca ccatctggtt ggtgttggca gtaccgtaga
    12361 aagtacatgt tcccggcgca tggtaagacg cggcttctga ctccagtaag gccatgcggt
    12421 ccactttacc ttcggcataa agctggcgaa tacgcacttt ttctttattt ggcaaaccgc
    12481 ttgccatcgg tccagacggc acaaacaccg caggcaaatg accaaacgac agggctgcca
    12541 tcgtcagacc cgggacaatc ttgtcgcaca caccgaggaa cagagcacca tcaaacatgt
    12601 tatgggacag ccccaccgcc gcagacatcg ctatcacttc gcggcttagc agcgacaatt
    12661 ccattccatc ctgcccctgg gtgacaccat cacacatcgc cggaacaccg cccgcaacct
    12721 gaccaaccgc attcgcttca tgcagggctt tacgaatgat ttctggatag tgttcataag
    12781 gctggtgcgc ggagagcatg tcgttatagg aggtgatgat ggcgatattg ttacgcaaca
    12841 tgcttttcaa agaggctttg tcttctggct ggcaggcagc gaaaccgtgt gccaggttac
    12901 cgcatgccaa ctgcgaacga tgaacggtcg acgttttcgc ttgttctatc cgggcgagat
    12961 aagcagagcg agtctcgcgc gaacgttcaa tgattcgatt tgttacgcgt aacaattgtg
    13021 gattcataaa ggctcctgaa attgagttgt cagagcagga tgattcacaa cgcgtttcat
    13081 tcagaggatt tatgagtgaa acgcctgtaa ccggagctca tagggcaaaa acgtttcagt
    13141 cagtgtaata aaaaaagcct cgtgggtgaa tccgcacgag gcctgaaagt gtaaaaattg
    13201 ttctacaatc tgtgcaagat tatgttaccg gtaaaataac cataaaggat aagcgctgat
    13261 attactcaaa ctcattccag gaacgaccat cacgggtaat catcgccacc gaggcaacgg
    13321 gtccccaggt tccggcctga tacggtttcg gcgcatcatt gtccatcgcc cacgcctcag
    13381 taatggagtc tacccatttc caggcttctt ccacttcgtc gcgacgtaca aacagtgcct
    13441 gaataccacg catggtttcc agcagcaaac gttcataggc atccgccaga tgcgtctgat
    13501 taaaggtttc tgaatagctc agatccagct tggtgatttg caggttatgt ttgtggtcaa
    13561 ggccaggaac tttattcagt acctggatat ccacgccttc atcaggttgc agacggatag
    13621 tcagtttatt ctgcggcaga tcctgccacg actctttaaa cagattcagt tcaggtgttt
    13681 tgaaatagac cacgacttca gaacatttgg tcggcagacg tttaccagta cgcaggtaga
    13741 atggcacacc ggcccagcgc cagttatcaa tgtcgacgcg gatcgccacg aaggtttctg
    13801 tattgctact tttgttcgcg ccctcttctt ccagatatcc cggcactttt ttgccctggg
    13861 cgaagcccgc agtatattgc ccgcgtacgg ttttttcgcg tacgttggag cggtcgatgc
    13921 ggcgcaggga cttcagtact ttcacttttt catcgcggat gctgtctgcg ctcaggtcag
    13981 atggcggaga catcgcaatc atgcagagaa tttgtagcag gtggttctga atcatgtcac
    14041 gcatctgacc ggctttatca aaatagcccc agcgcccttc gatccccact tcttctgcca
    14101 cggtaatctc aacatgatca atggtgcgat tgtcccagtt attcacaaac agggagttag
    14161 caaaacgcag cgccaacagg ttcagcaccg tttctttacc aagatagtgg tcgatacggt
    14221 aaacctggca ctcctcgaag tattcgccaa cctgatcatt gatttcctgc gaggtcgcca
    14281 gcgacgtccc cagcggtttc tccatgacta cgcgtgccgg tttagcattc agttttgcct
    14341 cgccaagccc tttgcaaatt gcgccaaaag tgctgggcgg catggcaaag tagttaatag
    14401 tgatacgatt tttttgatcc agcatcgcgc cgagacggtt gaatgcagca gtgtcattga
    14461 catcgagatt acaaaaatcc agacgtgcac tcagggtgtc ccataaacct tcatcaatgg
    14521 tttctttcat gaaagtttcg agcgcctcgc ggacaacttt ggtatatgcc gctttatccc
    14581 agtcagcacg ccctacgccg ataatccggg tgtccgggtt gagctgaccg gctttttcca
    14641 gttgatacag ggaaggcagc aatttacgac gcgcaaggtc gcctttcgcg ccgaaaatga
    14701 ccaggtcaca ggcctgggct gtttgcgtta ccgccatgtc attctcctta agttaactaa
    14761 cccggtactt aagccagggt atacttgtaa ttttcttacg gtgcactgta ctgcttttcc
    14821 gagcttgcga aaactgtaaa cgcttatcca cccgtgcgat tacgggaaaa gcgcgcaaag
    14881 tgcggcaaaa ctgataaaaa aatcatcgtt ttccattcgg taaaaatctg acactgatca
    14941 tgttatgaaa aaaaataaca acttttttat ctgcttttgt cattaacacg gcacacaggc
    15001 gtaatatctg acaaaactgc atttcgattt ctttcagtgc ggaaatcgtc attacccgtg
    15061 agtctcttta catcatgaat atgctggaaa aaatccagtc tcagctggaa catttgagca
    15121 aatcagagcg caaagttgcc gaggtcattc tggcttcgcc cgataacgcg atccattcga
    15181 gtattgctgc tctggcgctg gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc
    15241 gcagcatgga cacgcgcggt tttcctgatt ttaaacttca tctggcacaa agtctggcga
    15301 atggcactcc ctatgttaat cgcaacgtca atgaagatga cagcgttgaa tcatacacgg
    15361 ggaaaatatt tgagtccgca atggcaacgc tcgatcatgt ccgtcattca ctggataaat
    15421 ctgccatcaa ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat
    15481 taggctcttc tgccgccgtt gcccacgatg cgatgaataa gttctttcgt tttaatgttc
    15541 cggtggtgta ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg
    15601 gagacgtggt ggtgctgatt tctcacacag gaagaacaaa aaatctggtc gagctggcgc
    15661 agctggcacg cgaaaacgac gccatggtga ttgccctcac ctctgcgggt accccgctcg
    15721 cccgggaagc aacgctggca attaccctcg acgtaccgga agatactgac atttatatgc
    15781 ccatggtttc tcgacttgca cagctgaccg tgatagatgt gctggcgaca ggatttactt
    15841 tgcgacgcgg tgcaaaattc agagataact tgaagcgggt caaggaagcg ctgaaggaat
    15901 cgcgttttga taagcagtta cttaatttaa gtgacgatcg ctaaaagcga ctgtcactgt
    15961 cctaatctta tacgacatcc gaatgagatt aatttatcgc catcgcggcg ttatttcatt
    16021 cggatttcat gttcaagcaa cacctggttg tttcagtcaa cggagtatta catgtccaaa
    16081 aggcttcgca gaacaaaaat cgttaccacg ttaggcccag caacagatcg cgataataat
    16141 cttgaaaaag ttatcgcggc gggtgccaac gttgtacgta tgaacttttc tcacggctcg
    16201 cctgaagatc acaaaatgca cgcggataaa gttcgtgaga ttgccgcaaa actggggcgt
    16261 catgtggcta ttctgggtga cctccagggg cccaaaatcc gtgtatccac ctttaaagaa
    16321 ggcaaagttt tcctcaatat tggggataaa ttcctgctcg acgccaacct aggtaaaggt
    16381 gaaggcgaca aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga cgtcgtgcct
    16441 ggtgacatcc tgctgctgga cgatggtcgc gtccagttaa aagtactgga agttcagggc
    16501 atgaaagtgt tcaccgaagt caccgtcggt ggtcccctct ccaacaataa aggtatcaac
    16561 aaacttggcg gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc agacattaag
    16621 actgcggcgt tgattggcgt agattacctg gctgtctcct tcccacgctg tggcgaagat
    16681 ctgaactatg cccgtcgcct ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag
    16741 gttgaacgtg cggaagccgt ttgcagccag gatgcaatgg atgacatcat cctcgcctct
    16801 gacgtggtaa tggttgcacg tggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc
    16861 ggcattcaga aagcgttgat ccgtcgtgcg cgtcagctaa accgagcggt aatcacggcg
    16921 acccagatga tggagtcaat gattactaac ccgatgccga cgcgtgcaga agtcatggac
    16981 gtagcaaacg ccgttctgga tggtactgac gctgtgatgc tgtctgcaga aactgccgct
    17041 gggcagtatc cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa
    17101 atcccgagca tcaacgtttc taaacaccgt ctggacgttc agttcgacaa tgtggaagaa
    17161 gctattgcca tgtcggcaat gtacgcagct aaccacctga agggcgttac ggcgatcatt
    17221 accatgaccg aatcgggtcg taccgcgctg atgacctccc gtatcagctc tggtctacca
    17281 attttcgcca tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta tcgtggcgtt
    17341 acgccggtgc actttgatag cgctaatgac ggcgtagcag ctgccagcga agcggttaat
    17401 ctgctgcgcg ataaaggtta cttgatgtct ggtgacctag tgattgtcac ccagggcgac
    17461 gtgatgagta ccgtgggttc tactaatacc acgcgtattt taacggtaga gtaagtacgt
    17521 tgccggatgc ggcgaaaacg ccacatccgg cctatagttc aattatagtt caacagattt
    17581 cgaatattct gaagcaaact tgaacttatc atcaggcgaa ggcctctcct cgcgagaggc
    17641 ttttttattt gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc
    17701 gagttttcag caattttagt atccaggtgt attgttctgg tcgcggacca acaaaaatct
    17761 cgacttcttc attcatccgc cgctcaatcg tatgatcatc cgcctctaac agatcatcca
    17821 tcggtgggcg cacctgaatc gtcagacgat gcgtcttgcc atcataaatc ggaaacagcg
    17881 gtacaacgcg cgcacggcat actttcatca aacgaccaat cgcgggcaac gtcgctttat
    17941 aggtggcaaa gaaatccaca aattcgctgt gctctgggcc atgatcctga tcgggtaaat
    18001 aatatcccca gtacccctga cgtaccgact ggatgaatgg tttaataccg tcatttctcg
    18061 catgcagacg accgccaaag cgacgacgca ccgtgttcca gacataatca aaaaccgggt
    18121 tgccctgatt atggaacatc gctgccattt tctgcccttg cgaggccatc agcatggcag
    18181 gaatatcgac ggcccaaccg tgcggcacca gaaagataac tttctcgtta ttacgccgca
    18241 tctcttcgat gatctccagc ccttgccagc caacgcgcgg ctgaattttc tccggcccgc
    18301 gtattgccaa ctcagccatc attgccatcg cttgcggcgc ggtggcaaac atctcatcaa
    18361 caatcgcttc gcgttcagct tcactacgtt ctggaaagca gagcgacaga ttgattaacg
    18421 cacgacggcg tgagcttttt cccagtcgtc cggcaaaacg tcccagccgt gccagaatgg
    18481 gatcacggaa ctttggcggc gttaaagcga tacccgccat cgctgctaca cccagccatg
    18541 ctccccagta gcgcgggtgg cgaaaggatt tatcaaactc aggaatgtat tcgctattat
    18601 tttttttcgt ttccatgctt ttccagtttc ggataaggca aaaatcaatc tggtgatagt
    18661 gtagcggcgc aacttgcccc gcaccaaata aaaaaagccg gtactgactg cgtaccggct
    18721 gcgaatggat gttaattaat caaacagtag ctgcggcaca atctctttgg cctgcgccag
    18781 gaattcgcga cgatcggagc cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg
    18841 gtttacggcc tgctggttta tccatacttc atagtgcaga tgcggcccag ttgaacgtcc
    18901 ggtattaccg gaaagcgcga tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag
    18961 aatcttgcgc aagtgcatat aacgcgtggt gtagctgcga ccatgacgaa tagccacata
    19021 ataacctgct gcgccactgc gtttggcaac caccacttca ccgtcaccca ctgaaagcac
    19081 tggcgtacct tgtggcatgg cgaaatcaac acctctgtgt ggtgcaacgc gacctgtcac
    19141 cggattagtt cgacgcgggt taaagttaga cgagatacgg aactgtttcg ccgtcgggaa
    19201 tcgcaagaat cctttcgcca gaccagtacc gttacgatcg tagaatttgc catcctcagc
    19261 gcggattgcg taataatctt taccttctga acgcaaacgt acgcccagca gctggctttg
    19321 ctcacgttta ccatcaagca tttctcgaga cattaatacc gcaaattcat cgcctttttt
    19381 cagtttgcgg aaatccattt gccactgcat ggctttaatc actgcgctca cttcggcgct
    19441 ggttaaaccg gcgtttctgg cgctggcaac aaagctccct ccgacggtac ctttcagcag
    19501 attgttaacc cactctcctt gctgcatttc gctggtcatt ttaaaaccgt tagcggcagt
    19561 acggtcatag gttcgggttt cacgacgaga cacttcccag gtgaggcgct gcagttcgcc
    19621 gtccgcggtt aatgtccagg agagttgttg accgattttc aggttacgca attctttgtc
    19681 ggcagcagcg agttgggtga tatcacccat atcaatacca tactgattga gaatgctgct
    19741 tagcgtatcg ccagtggaaa caacatattc atgcacgccc gcttcaccgg cgattttgtc
    19801 atccagttcg tcctggggaa tggcttcatc ttcttgtgca gcttgatcaa tcggctcact
    19861 ggcttcaggt aagagcgaac gaatttcgtt ctgttccagc tcaatggttt tgacaattgg
    19921 cgtggcatca cggtgataaa catagggccg ccagacagcg acggccagag taagaacggt
    19981 gagcgacccc aacataacgc ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga
    20041 gcgggctatc tgttgcacgt aatcacttcc tcattaatct cctttcaggc agctcgcata
    20101 ctggttggct aattgactca ggaattctga atagcttgtt ttacccagtt tgatattcgt
    20161 ccccagggga tccaacgttc ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac
    20221 cgctggcctg aactgtggct cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt
    20281 tcttatttca tgtaaacgct gcgcgccagg ttgaatctca gggttaacgg taaaatgacc
    20341 aagcggtgtc agtccgaact gtttttcgaa atagccgtaa gcatcgtgaa aaacgaaata
    20401 acctttcccc ttgagcggcg cgagctcgtt accaacctgc gtttcggttg aggccaattg
    20461 tgcctcaaaa tccttcaggt tggcgtcaag tttggctcga ctttgcggca taagttccac
    20521 taattttcca tggattgcaa ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg
    20581 catgttgaaa tcgccgtgat ggtgatcttc gtcacttttt tccgcgtggt cgtgatcatc
    20641 atcatcgccg tgaatacttt tcatcagtag cggtttcaca tcttcaagct gcgcaatcgt
    20701 tacctgcttc gcttctggta atttgcttac cggtttttgc ataaacgctt ccatctccgg
    20761 gccaacccaa acgactaagt ccgcgttctg taagcgtttt acatccgatg gacgcagtga
    20821 ataatcatgt tctgaagcgc cgtcaggcag taaaacctcc gtttctgtta caccatcagc
    20881 aatggcagaa gcgatgaacc caacgggttt aagcgaagcg acaacggcgg catctgcggc
    20941 ctgtgttgca cctccccaga gagcggcgga taatgctgcg aaaagaagcg tttttttatg
    21001 taacataatg cgaccaatca tcgtaatgaa tatgagaagt gtgatattat aacatttcat
    21061 gactactgca agactaaaat taacatgaca agtctggttt ccctggaaaa tgtctcggtt
    21121 tcttttggcc aacgccgcgt cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt
    21181 ttgactttac ttgggccaaa tggcgcaggt aagtcgacac tggtacgggt agtgctcggg
    21241 ctggtaacac ccgatgaagg ggttatcaag cgcaacggaa aactgcgcat cggctatgta
    21301 ccgcagaagc tgtatctcga caccacgttg ccactgaccg taaaccgttt tttacgctta
    21361 cgccctggta cacataaaga agatattttg cctgcactga aacgtgtcca ggccgggcac
    21421 ctgattaacg caccgatgca aaagctctct ggtggcgaaa cgcagcgtgt actattagcg
    21481 cgagcattgt taaattgccc gcaattatta gtgctggatg aacccactca aggcgtggat
    21541 gtaaatggcc aagtggcgtt atatgacctt attgaccagt tgcgtcgtga actggattgt
    21601 ggcgttttaa tggtttctca cgatctgcat ctggtgatgg caaaaaccga tgaagtgctg
    21661 tgcctgaatc accacatttg ttgttccggc acaccggaag ttgtttccct gcatccggag
    21721 tttatttcaa tgtttggtcc tcgtggtgct gaacaactgg gtatctatcg ccatcatcat
    21781 aatcatcgtc acgatttaca gggacgaatt gttttgcgtc ggggaaatga tcgctcatga
    21841 ttgaattatt atttcccggt tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc
    21901 tgggttcgtt tgtagtctgg cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct
    21961 cattacttgg tgtcgcgttt ggtttgttgc tggacgtgaa tccattctat gcggtgattg
    22021 ccgttacgct gctgctggcg ggcggtctgg tatggctgga gaagcgtcca cagctggcga
    22081 tcgacacgtt attagggatt atggcgcaca gtgccctgtc gctgggccta gtggtcgtta
    22141 gtctgatgtc taacattcgt gttgatttga tggcttacct gttcggtgat ttactggcag
    22201 tgacgccaga agatctcatc tctattgcga ttggcgtggt catcgtggtg gctattttgt
    22261 tctggcaatg gcgcaatttg ctgtcgatga cgattagccc ggatctggcg tttgttgatg
    22321 gtgtgaaatt acagcaagtg aaattgttgt tgatgctggt gacggcattg acgattggtg
    22381 tagcgatgaa atttgtcggt gcgttgatta ttacttcgtt gctgattatt cctgcagcta
    22441 ctgcgcgtcg ctttgcccgt acgccggaac agatggctgg tgtcgctgtt ttggtgggga
    22501 tggtggcagt aactggcggt ttaacctttt ccgcgtttta cgatacgccg gcgggtccgt
    22561 cggtggtgct atgtgcggca ctgttattta ttatcagtat gatgaaaaag caggccagct
    22621 aatctatcgc tgaacacatt tgtcggatgc ggcgcgagcg ccttatccga cctacggttc
    22681 gctatctctg gctggcctga taagacgcga acagtgtcgc atcagacaca ctgccagtgt
    22741 cggatgcggc gcgagcgacc aatccgactt acggcatttc tggcggcgtt atgccaaagt
    22801 gattccacgc ccgcgtcgtc gccatacgcc cgcgcggtgt acgctgcaaa aagccttgct
    22861 gaatcaaata aggttccagt acatcttcaa tggtttcacg ttcttcgcca atggctgccg
    22921 ccaggttatc cagacctacc ggcccaccaa agaacttatc gattaccgcc agcaacaatt
    22981 tgcggtccat ataatcgaaa ccttcagcat cgacattcaa catatccagc gcctgagcag
    23041 cgatatctgc cgagatggtg ccatcgtgct tcacttcggc gaaatcacgc actcgacgca
    23101 gcagacggtt ggcaatacgt ggcgtaccgc gagcgcgacg agctacttcc tgcgcaccgt
    23161 catcactcat ctcaagcccc ataaagcgtg cactgcgact gacgatatat tgcagatccg
    23221 gcacctgata aaactccaga cgttgcacaa taccaaaacg gtcgcgcaac ggtgatgtca
    23281 gcgaacctgc gcgcgtggtt gcgccaatca gggtaaacgg cggcaaatca attttaatgg
    23341 agcgtgccgc cggaccttca ccaatcatga tatccagttg gtagtcttcc attgccgggt
    23401 acagcacttc ttcaacaact ggcgataaac ggtggatctc atcaataaac agcacgtcat
    23461 gcggttcaag gttagtgagc atcgcagcca aatcgcccgc cttttccagc accggaccag
    23521 aagtcgtgcg taaattaacg cccatttcat tggcgacaat gttggcaagc gtagttttac
    23581 ccaaccccgg aggaccaaaa atcaacaaat gatcgagggc atcgccgcgc agtttcgctg
    23641 ctttgatgaa aatctccatc tgtgaacgaa cctgcggctg accaacatac tcttccagta
    23701 atttagggcg aatggcgcga tctgctacat cttccggcaa agtggtaccg gcagaaatca
    23761 gacggtctgc ttcaatcatc ctttacctca taacgcggcg cgtagggctt cgcgaattaa
    23821 tgtttcactg ctggcgtcag ggcgagcgat tttgctcacc atgcggcttg cttcttgtgg
    23881 tttatagcct agcgccacca gcgcggcaac tgcttcttgt tcagcatcgt cggtcgccgg
    23941 gctggcagga gacgtgagta ccaggtcggc ggctggcgta aagagatcgc catgcaaacc
    24001 tttaaatcgg tctttcattt caacaatcaa gcgttcggcg gtttttttgc caatacccgg
    24061 cagtttcacc agtgccccca cttcttcacg ctcaacggca ttaacgaact gctgcgctga
    24121 cattccggag aggatcgcca gggccaactt cgggccgacg ccgttggttt tgatcaactc
    24181 tttgaacaac gtgcgttctt gtttattgtt aaaaccgtac agcagttgcg cgtcttcacg
    24241 caccacaaag tgggtgaaaa cgatcgcttc ctgacccgct tcagggagtt cataaaaaca
    24301 ggtcatcggc atatgcactt catagcctac gccgcccact tcaattaaca ccagcggggg
    24361 ttgtttttca atgatgatgc ctctgagtct gcctatcaca tgacgctcct gcgtaatgaa
    24421 tcaaagataa tgctgtatga taaaaaaatg ctggatagat atccagcgta ggatgaagaa
    24481 aacttgcgag gtgtcccgat gatctgaaaa atggcacagt ataatttatt caacaggtta
    24541 tattggaagc aaatatttga atattacata ttcagtgaag aaatgcgtaa taaaaatata
    24601 cattgcgact cctgaaaaaa taattttttt atgctattac gtatattcgt atctatttca
    24661 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    24721 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    24781 tcacgtcggg atcattatcg gtcataacgg tgaaga
//
LOCUS       DAJTYF010000049        24804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTYF010000049 DAJTYF010000000
VERSION     DAJTYF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.049"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(51..884)
                     /gene="fdhD"
                     /locus_tag="OO590_002136"
     CDS             complement(51..884)
                     /gene="fdhD"
                     /locus_tag="OO590_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6637500.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1078..4128
                     /gene="fdnG"
                     /locus_tag="OO590_002137"
     CDS             1078..4128
                     /gene="fdnG"
                     /locus_tag="OO590_002137"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1663..1665,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6637501.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMFSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGITLTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4141..5043
                     /gene="fdoH"
                     /locus_tag="OO590_002138"
     CDS             4141..5043
                     /gene="fdoH"
                     /locus_tag="OO590_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6637502.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5040..5675
                     /gene="fdoI"
                     /locus_tag="OO590_002139"
     CDS             5040..5675
                     /gene="fdoI"
                     /locus_tag="OO590_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6637503.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5672..6601
                     /gene="fdhE"
                     /locus_tag="OO590_002140"
     CDS             5672..6601
                     /gene="fdhE"
                     /locus_tag="OO590_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6637504.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6931..7173)
                     /locus_tag="OO590_002141"
     CDS             complement(6931..7173)
                     /locus_tag="OO590_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637505.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7391..7609)
                     /locus_tag="OO590_002142"
     CDS             complement(7391..7609)
                     /locus_tag="OO590_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6637506.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(8288..9421)
                     /locus_tag="OO590_002143"
     CDS             complement(8288..9421)
                     /locus_tag="OO590_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637507.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKP
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATVVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKNLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            complement(9591..9929)
                     /locus_tag="OO590_002144"
     CDS             complement(9591..9929)
                     /locus_tag="OO590_002144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637508.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(10519..11460)
                     /gene="fabY"
                     /locus_tag="OO590_002145"
                     /gene_synonym="yiiD"
     CDS             complement(10519..11460)
                     /gene="fabY"
                     /locus_tag="OO590_002145"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6637509.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(11505..11942)
                     /gene="dtd"
                     /locus_tag="OO590_002146"
     CDS             complement(11505..11942)
                     /gene="dtd"
                     /locus_tag="OO590_002146"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6637510.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11939..12811)
                     /locus_tag="OO590_002147"
     CDS             complement(11939..12811)
                     /locus_tag="OO590_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6637511.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12805..13404)
                     /gene="yihX"
                     /locus_tag="OO590_002148"
     CDS             complement(12805..13404)
                     /gene="yihX"
                     /locus_tag="OO590_002148"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6637512.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(13503..14288)
                     /locus_tag="OO590_002149"
     CDS             complement(13503..14288)
                     /locus_tag="OO590_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6637513.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(14322..15218)
                     /gene="yihV"
                     /locus_tag="OO590_002150"
     CDS             complement(14322..15218)
                     /gene="yihV"
                     /locus_tag="OO590_002150"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR6637514.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGVKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            16151..17041
                     /gene="yihT"
                     /locus_tag="OO590_002151"
     CDS             16151..17041
                     /gene="yihT"
                     /locus_tag="OO590_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR6637515.1"
                     /translation="MPLGAASREVYSQARASGGFAMLAVDQREAMRMMFAAAGAPAPV
                     ADSVLTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNG
                     IPVDSVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVS
                     IIEPVVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRL
                     NDHINMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDV
                     CAPKLQQLGDIVDEMMAKRR"
     gene            17058..18299
                     /gene="yihS"
                     /locus_tag="OO590_002152"
     CDS             17058..18299
                     /gene="yihS"
                     /locus_tag="OO590_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR6637516.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            18437..19339
                     /locus_tag="OO590_002153"
     CDS             18437..19339
                     /locus_tag="OO590_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR6637517.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            19538..21574
                     /gene="yihQ"
                     /locus_tag="OO590_002154"
                     /pseudo
     CDS             19538..21574
                     /gene="yihQ"
                     /locus_tag="OO590_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            21620..23005
                     /locus_tag="OO590_002155"
     CDS             21620..23005
                     /locus_tag="OO590_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6637518.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            23048..24451
                     /locus_tag="OO590_002156"
     CDS             23048..24451
                     /locus_tag="OO590_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6637519.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            24519..>24804
                     /gene="ompL"
                     /locus_tag="OO590_002157"
     CDS             24519..>24804
                     /gene="ompL"
                     /locus_tag="OO590_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR6637520.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLI"
BASE COUNT         6141 a         6380 c         6091 g         6192 t
ORIGIN      
        1 cgaaatgaat aagtcctgca atttaatata tatcgcagga cttattttaa ttaattgctc
       61 aaacgctgcg gatgggtata aaccgttgcc cgacccggtt tacaaaaacc taccagtgtc
      121 agattacagc gctcagccac ttctacagca agcgtggtcg cggcagacac cgcaaacaaa
      181 atctctacgc cgcacatcgc cgacttttgc accatttcat aactggcacg actggaaacc
      241 agtaccgcac cttgctgcca gctttcccct tcttgtgacc ggcggcctaa cagtttgtcc
      301 agcgccacat ggcgacccac gtcttcatgc ccgccgacca gttcgccaga tggcaacatc
      361 caggcagcgg cgtgagtaca accagtcagt tgccccactg gctgaaaatc gttgagatga
      421 cgtaatgcat catccagttt gttgagatca aacgtctggg tgaacggtag cggctgcacc
      481 ggttttccga tgtcattaag ttgctccacg ccgcatacgc cacagcccgt acgtcccgcc
      541 agcgcccggc ggcgctcctt caaccccata aagcggcggc tggaaagctc aatttgtact
      601 tcaagaccat tacaggaagg aacgacatcc atgccgaaga tatcgcgcgg actttctatt
      661 atcccctcgg aaagcgaaaa accgagcgca aagtactcaa ggtctttggg cgacgccatc
      721 atcaccacat gcgaaatgcc gttgtagacc agcgcaacgg gaacttcttc cgcgacctca
      781 tctaaccgtg ggtgttgtaa atcatcacgc cgccataact caatttgacg aacacctgtg
      841 atatttgtca cattttcgat ttcttttcgc tgtgtttttt tcactttctc ttaaccagat
      901 aagaacacac acaccaacat tatggtattc tgttacaaac ccttcctaga tggagggaaa
      961 ttgagccaat tctggacctt tgcggcccct tccgcaaaga aaaataactc ccactccctg
     1021 cacacgcagc aagcgaatgt aaatgggacg tgacaatgtc gaaacaagga gcaatccatg
     1081 caggtcagca gaaggcagtt ctttaagatc tgcgctggcg gtatggcagg caccacggca
     1141 gcggcactgg gttttgcacc cagcgtagca ctcgcggaaa cccggcagta taaactgctg
     1201 cgcacccgcg aaacccgtaa tacctgcacc tattgttccg taggctgtgg gctgttgatg
     1261 ttcagcctcg gtgacggagc aaaaaacgcc aaagcatcta tcttccatat cgaaggcgat
     1321 ccggatcacc cggtcaaccg cggtgcactt tgtccgaaag gcgctggcct ggtggatttc
     1381 atccactccg aaagccgtct gaagtttccg gaataccgtg cgccaggttc tgataaatgg
     1441 cagcaaatca gttgggaaga ggcgtttgat cgcatcgcca aactgatgaa agaagaccgc
     1501 gatgctaact acattgcgca aaacgccgaa ggcgtgactg ttaaccgctg gctctccacc
     1561 gggatgctgt gtgcttccgc gtcgagtaac gaaaccggct atttaacgca aaaattctcc
     1621 cgcgcgctgg gtatgctcgc ggtcgacaac caggcgcgtg tctgacacgg accaacggta
     1681 gcaagtcttg ctccaacatt tggtcgcggt gcgatgacca accactgggt cgacatcaag
     1741 aacgccaacc tcgtcgtggt gatgggcggt aacgccgctg aagctcaccc ggtcgggttc
     1801 cgctgggcga tggaagccaa aattcacaac ggcgcgaagc tgattgtgat cgatcctcgc
     1861 tttacgcgta cggcggcggt agctgactac tatgccccta ttcgttccgg tactgacatt
     1921 gctttcctgt caggcgtatt gctgtacctg ctgaacaatg aaaaattcaa ccacgaatac
     1981 accgaagcct ataccaacgc cagcctgatc gtgcgtgagg attacggctt tgaagatggc
     2041 ctgttcaccg gctacgacgc ggaaaaacgc aagtacgata aatcctcctg gacttatgaa
     2101 ctggacgaaa acggcttcgc caaacgcgat accacgctgc aacatccgcg ctgcgtgtgg
     2161 aacttgctga aacagcacgt ttcccgctac acgccagatg tggttgaaaa catctgtggt
     2221 acgccaaaag acgcgttcct gaaagtctgc gaatacatcg cagaaaccag tgctcacgat
     2281 aaaactgcct cgttcctgta tgccctcggc tggacgcaac actccgttgg tgcgcaaaac
     2341 attcgtacga tggcgatgat ccagctgctg ctcggcaata tggggatggc cggcggcggc
     2401 gttaacgccc tgcgcggtca ctccaatatt caggggctga cggacctggg gctactgtcg
     2461 cagagtctgc caggttacat gacgctgcca agcgagaagc agaccgatct gcaaacctac
     2521 cttaccgcca acacgccaaa accgctgctg gaaggccagg taaactactg gggcaactac
     2581 ccgaaattct tcgtctctat gatgaaggcc ttctttggtg ataaagcgac ggcggaaaat
     2641 agctggggct ttgactggtt gccgaagtgg gataaaggct acgacgtcct gcaatacttc
     2701 gagatgatga aagagggcaa ggtcaatggc tatatctgcc agggctttaa ccctgttgcc
     2761 tcattcccga acaaaaacaa agtgatcggt tgtctgtcga aactgaagtt cctcgtcacc
     2821 atcgacccac tgaacactga aacctctaac ttctggcaga accacggtga gctgaacgaa
     2881 gttgactcgt cgaagatcca gaccgaagtg ttccgtctgc catcgacctg cttcgcagaa
     2941 gagaacggtt caatcgttaa ctcaggtcgc tggttgcagt ggcactggaa aggtgcggac
     3001 gccccgggga ttacgctgac tgacggtgag atcctctccg gtatcttcct gcgcttgcgc
     3061 aagatgtatg ccgaacaggg tggcgcgaac ccggatcagg tgctgaacat gacctggaac
     3121 tacgccattc cgcatgagcc gaagtcggaa gaagtagcga tggagagcaa cggtaaggcg
     3181 ctggccgata ttaccgatcc ggcaaccggt gcggttatcg tcaagaaagg ccaacaactt
     3241 agctcgttcg cccaattgcg tgatgacggt acaacctcct gtggctgctg gattttcgcc
     3301 ggtagctgga cgccggaagg caaccagatg gcgcgtcgtg ataacgccga tccgtctggc
     3361 ctcggtaaca cgctgggctg ggcatgggca tggccgctta accgccgcat tctgtataac
     3421 cgcgcctccg cagatccgca gggtaacccg tgggatccga agcgtcagtt gctgaaatgg
     3481 gacggcacta agtggaccgg ctgggatatt ccggactaca gcgcagcgcc tccgggtagc
     3541 ggcgtcgggc cgtttatcat gcagcaggaa ggcatggggc gtctgtttgc cctcgataag
     3601 atggcggaag gtccgttccc ggaacactac gagccgtttg aaacgccgct gggaactaac
     3661 ccgctgcatc caaacgttat ctcgaacccg gctgcacgta tctttaaaga cgacgccgaa
     3721 gcattgggta aagccgataa gttcccgtat gtcggaacca cctatcgtct gaccgagcac
     3781 ttccactact ggaccaaaca cgcgctgttg aacgcgattt tgcagccaga gcagtttgtg
     3841 gaaatcgggg agtcgctggc gaataaactt ggcattgccc agggcgatac cgtgaaagtc
     3901 tcctccaacc gtggctatat caaagccaaa gcggtggtga ccaaacgtat tcgcacgctg
     3961 aaagcgaacg gcaaagatat cgataccatc ggtattccga ttcactgggg ctatgaaggc
     4021 gtagctaaaa aaggctttat tgccaatact ttaacgccat tcgttggtga tgcgaacacg
     4081 cagacgccgg agtttaagtc cttccttgtg aatgtggaaa aggtgtaacg gagacgacat
     4141 atggcttatc aatctcaaga tatcattcgt cgttccgcga ctaacggtct gacccccgcg
     4201 cctcaggcgc gggacttcca ggaagaagtg gcgaaactca tcgacgttac cacctgtatc
     4261 ggctgtaaag cctgtcaggt ggcgtgttca gagtggaacg acattcgcga taccgtcggc
     4321 aataacattg gggtgtacga caaccccaat gatttaagcg ccaaatcgtg gacggtaatg
     4381 cgcttctcgg aagtggagca gaacgacaaa ctggaatggc tgatccgcaa agacggctgt
     4441 atgcactgtt ccgatccagg ctgcctgaaa gcgtgcccgg cggaaggtgc aattattcag
     4501 tatgccaacg gtatcgtcga cttccagtcc gagcagtgca ttggctgcgg ttattgcatt
     4561 gcgggctgtc cgttcgacat tccgcgcctc aacccggaag acaaccgcgt ctacaaatgt
     4621 acgctgtgcg ttgaccgcgt ggtggttggg caagaaccag cctgcgtgaa gacctgccca
     4681 acgggcgcga ttcacttcgg tacgaaagag tcgatgaaaa cgctggcgag cgagcgcgtt
     4741 gctgagctga aaacccgcgg ttacgacaat gcgggtctgt acgatccggc aggcgtcggt
     4801 ggtacacacg tcatgtacgt gctgcaccat gctgacaagc caaatctgta tcatggcttg
     4861 ccggagaacc cggaaatcag cgaaaccgtg aaattctgga aaggcatctg gaaaccgctc
     4921 gcggctgttg gctttgcggc tacctttgcg gccagtatct tccactacgt gggtgtcggt
     4981 ccgaaccgtg cggatgagga agagaataat ctgcacgaag agaaagacga ggagcgcaaa
     5041 tgaaacgacg tgacaccatc gtgcgctaca ccgcgccgga acgtatcaac cactggatca
     5101 ccgccttctg cttcatcctg gcggcggtga gcgggctggg ctttttgttc ccgtccttca
     5161 actggttgat gcaaatcatg ggcacaccgc agctggcgcg aattctgcac ccgtttgtcg
     5221 gcgtggttat gtttgcctcg ttcatcatca tgtttttccg ttactggcat caaaacctaa
     5281 tcaatcggga tgatatcttt tgggcgaaga atattcgtaa gatcgtcgtc aacgaggaag
     5341 taggtgacac cgggcgttat aacttcggtc agaaatgcgt tttctgggcg gcgattattt
     5401 tcctggttct gctgctggtg agcggcgtga ttatctggcg tccttatttt gcgcctgctt
     5461 tctcaatccc ggtgatccga ttcgcgttaa tgctgcattc atttgccgca gtggcgttaa
     5521 ttgtggttat catggtgcat atctacgccg ccctttgggt gaaaggcacg attaccgcca
     5581 tggtggaagg atgggttacc agcgcctggg cgaagaaaca tcacccgcgc tggtaccgtg
     5641 aggtccgcaa gacaacggaa aagaaagctg aatgagtatt cgcataatcc cgcaagatga
     5701 gctgggttcg agcgagaaac gtacggcgaa tatgattccg ccgttattgt tccctcggct
     5761 caagaattta tacaaccgcc gcgccgagcg tctgcgcgag ctggcagaaa ataatccgct
     5821 gggtgattac ctgcgctttg ctgcgcttat cgcccacgcc caggaagtgg tgctgtacga
     5881 ccatccgctg gagatggatc tgaccgcgcg cattaaagaa gccagcgcac agggcaagcc
     5941 cccgctggat attcacgttc tgccgcgtga taagcactgg caaaagctgc tgatggcgct
     6001 aattgctgag ctgaaacctg aaatgagcgg cccggcgctg gcagtgattg agaatctgga
     6061 gaaggcatcg actcaggagc tggaagatat ggccagcgcg ctgtttgcct ctgatttctc
     6121 gtccgtcagc agcgataaag cgccgtttat ctgggctgcg ctgtcgctct actgggcgca
     6181 gatggccaat ctgatccccg gcaaagcccg cgctgaatac ggcgaacaac gtcaatattg
     6241 cccggtatgt ggctctatgc cggtgtccag catggtgcaa attggcacca ctcagggtct
     6301 gcgttacctg cactgcaacc tgtgtgaaac cgaatggcac gtagtgcgcg taaaatgcag
     6361 caactgcgag cagagcggca aactgcatta ctggtcgctg gatgacgaac aggccgcgat
     6421 taaagccgaa agctgcgatg actgcggcac ttacctgaag attctttatc aggaaaaaga
     6481 accgaaagtt gaagccgtgg cagatgacct cgcctctctg gtactggacg cgcgaatgga
     6541 gcaagaaggc tacgcccgta gttccattaa cccgttcctg tttccgggtg aaggagagta
     6601 atctctgacg ttttccgggt ggcgcaggtc gcccggatta cagctcatag ggtgtgacga
     6661 caccatctct cgtattccgc gttacccatt caagatcatg ccgcaattgt ctgtttgcct
     6721 cgttcttccc tccaacacat cgataaggtt atgggtatgt cactgatgac aagcgtatga
     6781 ttgcctgatg cgctgcgctt atcaggccta ccaataaact tcaatatatt gaatttgcat
     6841 gactttgtag gccggataag gcgttcacgc cgcatccggc atgaacaacg cgcacgttgt
     6901 cagcaatctg attgacatca ttttttccca ttaccattcc tcgcgcagct tacgctgata
     6961 ttcgacacca tcttcctcac acccttgcca gatgccagga acactggttc ttgctgtttg
     7021 cgattgattt atcaggtatt gatctaccgc ttcccttaat aggtccgcac gaggaagatt
     7081 acgctgcacc tcgagatcat caagttgctt aatcacctca ttcgataaat cgagtaaaat
     7141 tctgcccata tctatacctg ccagagagtt cataaatatc tcgccaatat catttttgaa
     7201 tctatggaga gaaaagtacc cttgccgaat ctttaaagaa agctcattta cgcatcattt
     7261 tttatttttg cgaggcgttt tccagatacg atgtaacagt tgcagttatt ataagttcaa
     7321 gtaaaacagc cttgtattaa taccagaata agggcatctg ttttttatat tcaagaatga
     7381 aaaatttttt tcattcctta tgctccttac actgatcatc aacaccaact acctccccct
     7441 tttgcctgcc tccctttgct ttacgcaccg gaaattgctg ccgctccaga tattgctcaa
     7501 ccgcttcgcg taaaattgca gatcgtgaaa ccttacgcca gccccgaagc ttgttcagcc
     7561 gtttaattgc ctcttctgat aaatgaagaa aaaccgtatt catcgccatt accaccacct
     7621 tacatatatt actcattaat gtatgtgcga aaattacgcc gaaaaagcat ttaaaaaaat
     7681 gcggattttt gcatgtcatt ttgtttttgc gaggcgtttt ccagaaataa taagagcgtt
     7741 gcagttgttt catttattac tcaaaaatgg tttagttatc gcccaggatg agttatgggt
     7801 gctatgaata tattatattc acggatattt atttgcataa tctgaatggt gtagccgaaa
     7861 aacgtcagca tcctaacagc accaggattt aaggtgaaat taatctttca tcacagaccg
     7921 gtcagtactg ccaccaaccg gtctttactt gctcatcgtt ataattttgt tggtaacagt
     7981 tcgggaaaca aggaattcct tccggaacac ccccggcatc atccgatagc attgacgtca
     8041 atcatcagcg cgacgatgag tttttttaat cgcatcatag ggtggcgacg ttcagcacga
     8101 ttacgggtca tagcaaaact cctgagcagg cggccactat gccgctttca ctggagttct
     8161 gaatactggt actttctgta tggcttcgcc atgcccctcg cggctaagta cctgcacaca
     8221 tctctctttg tatgtcattt ttgcaatgca ttgtccgaat tctgataatc ctaatgagtt
     8281 ttactcgtca gactcccatc cctacggtat acgagttata ttcatgaatg aaatcgacat
     8341 ttttaaaaaa cgaatcttgt aaaacatcag tgttgatatt accttgcaat ccaacattga
     8401 atttgttaga gacatgaaga actcgaatat caaaaaggtt ttttaacatc attaattctt
     8461 cgggtgttaa tgaatcgcaa ctaaaatatc cctgttgatc aaaaagattt tctatttctc
     8521 cactatcggc gaggcgtttt tcaaggagtg tttgcaacat cgagttgcca aggacaatgg
     8581 atatcgtgcg atctttcgca gatttacacc ccatcacaac ggcaacattc gtatccccct
     8641 caagagtttc atttatcgcc gtcgctaaaa tggtggtcaa acacgcaaac ggaactgtat
     8701 ttctttcgtg atttaattta tctaccagtt cattccattt atcaaaagcc gggctatatt
     8761 tgttgcgcac agaaatatcc gactggttaa acaattcagc catgcgattt tttatcatct
     8821 cgatatccgg cgaaccagaa agactttgcg cataatacgg tcctgatgat gttgcataca
     8881 ttaatgccaa gcccacaact gtcgccatga tgggcgcagc cacggttttg gcaagggcga
     8941 cgctgcgatg gctatgcgct tcacaggcta aaggaacata ttccactgac tgcggtaatt
     9001 ccttacatgc ctccctcatc attgcggtat gctttacaaa caacggtttt tcactgccca
     9061 aaaaaacttt ctgcttaatt gcactaagaa aacgcaaatc aaaaataatt gtttttggtt
     9121 tattattttt tggcacattt cctattatcc cgctaagaac cttccttgct gcatggagat
     9181 ttttttgcac agcacataac acaattgatg aagccttctc cactgcatta ttgattttaa
     9241 ttttaaaatc cgcatgatat acgttgacgg gcaatgcaag tatatctgga cctacatctt
     9301 ttgctgagta tttattggtg atcgacggac gtgaacccac gagaaaactt agctgcaaat
     9361 tatgctctat attatcagct gtttttgtgg tgtggttaaa catagagatg ccacctttca
     9421 ttctttcaca catatcctca actttttcac ttcgttcttg tggcaatttg gctttaacat
     9481 taatttgatg atttttaccc gtgcattcct taactacaag gggaatgtta tctaaagtgc
     9541 catacaggct aataacgtca ccaggatttt tatcatgtaa gtaagtttga ttaatacttg
     9601 aagcattcac aaagagggtc aatattataa ctgtttataa cttcacctac atcaagtgtg
     9661 tagtgcttac tctggagact gccatcctta tttaattgta tatcaatcaa tatatccccg
     9721 gactttgtca ccatatcttt gccataaacc atttgcatag ccatttcgag gagattttta
     9781 tctaactgat tgcgggcgtt gagtaagcga ttaccaaata atttatatac tgttcccgtc
     9841 attgccagcg ctgcgctttc tggtactttg cgtttccaca gtgaggtttc tctattttta
     9901 aacaggtcat attcattatt actcgacaaa ccatgcttca gttcgttaac acaatcgcac
     9961 agttgctcct caccttccat tttaaggcaa agtgaaaatt gatttaatgg cgtttacttc
    10021 ggtgatttat tactcattag ttgtttttct aaattaacaa cagaataact ttcttgttta
    10081 atgagattaa tattggcaac cattatagcc tccttcccac ggtttaagag caaacataca
    10141 acataatgat tcataacata cagagcagta aatgaatgat actgacaatg tatgccagcc
    10201 cattatcttt gcataatgtt tgtgagctat agattttaaa aatatttatg aaaaaagtat
    10261 ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac ctgaactaag atgatgagtt
    10321 aagcattatc tgcaataatt gccactcagc tctacaaata atatttaaat gctcctgaac
    10381 agtgatgtag caagacacgt atggtgccaa cgaagaataa aaatttataa agttcttatt
    10441 caatataacc ggaatagtca gggactgatg taaatgaccg gaatgaatga gagaggcagc
    10501 accgcccctc tccataagct actcttcttc gttcccgccc tcttcatacg ggccaaatgg
    10561 cttcgcgggc agaacgatat acgtgccttc aaacactgca cccggcgtct cgtcgccaaa
    10621 gatttcgacc tgcatctgca cccgtgcttt tcgtccgcgc gccagacggt cgagatcgcc
    10681 gcttaaggca ccaaggtcgg ctaccgcatg gggtttaccg ctaatcggtt tgctgtagcg
    10741 gatatgcgca tccgccagaa taatcgttcc gccgaggtgg cgttcacgca gcatcagcca
    10801 gataagcccc caaccggtga gcgtcgccag cgagaataaa ctcccggcaa acagcgtatg
    10861 gtgcggattc tgattgccgg tttccggcat ggtggtgata aatttttgcc cggtatattg
    10921 ttgaatacgc acgcccattt tttcactaag cgggatatgt tcgtaccacg cctgttgcag
    10981 ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga gtggcgacgg gcttaatcat
    11041 caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt tctccctgat taacaaaccc
    11101 cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta caggtcacgc gcttaacgcc
    11161 ttcctgacgc gccaccgact ccagggtcat cgccatcagc gttcctaacc ctttgtcctg
    11221 cacgtccggg tgaacggcca taaagcgaat ggacgcttca ttgtcggcat taatatacaa
    11281 tcggcctacc gccaccagat taccctgctc gtcgacgacc atctggtgat gcgccatcgc
    11341 atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg ggcttacgca acatttccca
    11401 gcgaaactga tagtaacgct ctaactcttc ttctgtttgt ggaacctgaa ggtgatacat
    11461 agcgatactc tctcttgttt cccgtgacaa ccctggaagc tggctcatac ctgcaaccag
    11521 aatgtcacgg ggccatcatt gaccagcgat acctgcatat cggcagcgaa gcgtcctgtt
    11581 tgcgtgttca tctcttgctg gcggcagcgt tcgacgaaat agtcatataa cgcctctgcg
    11641 cgatccggtg atgcgccttt ggagaaactt gggcgcatcc cccgttcggt atctgcggcg
    11701 agggtaaact gggaaaccac cagtacactg ccgcccgcct gttgcacgtt gagattcatc
    11761 ttgccttcgg catcgctaaa gatgcggtag ccgagcacac gctcgcacag acggtttgct
    11821 ttctgttcgt cgtcatcctt ttcgacaccc aataacacca aaagtcccgc gccaatttcg
    11881 cccgtcactt ctccctccac ggtgacgctg gcacgggtta cgcgttgaat taatgcaatc
    11941 atggttcgtc gtcttcttct tgttcagctg cttgttttag tttgcggtat tccccgagag
    12001 tgacagtaat ttccgcgcca agcaagacga tacaccatgt ccagtagacc caaacaaaga
    12061 gaatggggat caccgccagc acaccgtaaa tgagctgata tgacgggaac atggtgatat
    12121 aaagcgcgaa acctttcttt cctgcttcga acaggagtgc ggcgacaaac gcgccgacaa
    12181 tcgcgtcgcg gttaggtacg cggatggtag gaacaatgct gtacagcaac cagaaggaga
    12241 tccacgacaa cagcaacgga aaaatacgca gcacgttgtc gatgacagta ttgagatcgc
    12301 tcgcccagcg cagggagagc aaataggaac tgatcgccag actggcccct gccagcagcg
    12361 gccccagcgt taaaatcatc cagtacacgg cgaacgagta aatttttggt cgcgctcgtt
    12421 tactgcgcca gatggtattc aacgcgctat cgatggagta catcaacaat aacgcggtga
    12481 cgatcagccc gcacgcccca acggcggtca tcttgttgga attagcaaca aattgttcga
    12541 tataccgctg aataacatcg ccagtagcag gcagaaagtt ggcaaaaata aagtgacgta
    12601 actgaatgct gacgtcggaa aacatgggaa aagcggcgaa aagcgcaaaa acaacggcaa
    12661 ccagcggcac taatgagagc aacgacacat aggcaaggtt acctgccagg gttgtcatgt
    12721 tgtcctcatc aatacgttgc cagagtagtt ttagccaggc ccatagtgga cgggtacggt
    12781 gcctggcttt gtcctgaatg gtttttagca taacaccttc gcgaaatagt ccgggatggt
    12841 ggttttatct ttcaccagaa tactggtaat gcctagctga ttagctcctt ctatattatc
    12901 ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa ccttccgcct gcaaaacatg
    12961 ctggtaaatt cgtgcttcag gtttgcgcat ccccagatct tgcgacagat agatatggtc
    13021 agcagcatca cgaatttccg ggtattcttc cggccagaag gtggtatgca ggcggttggt
    13081 attggaaagc accaccacgc gatgcccctg ctcacgcagt ttatgcatga tggcgatcac
    13141 ttccgggcgc agcgcaacaa acaccgcctg ccagccgtga gagaactgct cgtagcttag
    13201 cggtagagcc atctcatgac acagcgcctc tgcgaacgct tcgtcgctaa tttccccacg
    13261 ctcatgctga tgaaacgcct cccccatatg aaaactcttc ttaagcgatg ccagcggaat
    13321 acgcgttaaa tcgctccagg ctcccagcac acggttaaag tcgatatcga caatcacatt
    13381 acctaaatca aagatataga gcatttttgc ctcctttcgc gccacaagaa attaactgta
    13441 gcgggaaagg atgggtttgg ctatgtggca aggtatgaag ccaggttaac ggtcacaaaa
    13501 atctacgcgt cttcctgggg aagaatgatt tcaatttggc tgtcttgtaa gcgtgatttt
    13561 agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga cctgtgccac gttaccaatt
    13621 tcaaccgccg ccgaagcatg atacttagtg tgatcggcga ccagcagaat atttctcgcg
    13681 tgcgccatca tcgttttcac cacgttagct tcgtttacat caaactccat caacgcgcca
    13741 tcgctctcaa tcgccccaac gcttgttacc agataatcag cacgaaaatc agccacaaag
    13801 gacgccgctg aagggccaat gatcccgcta ttatgagagc gcaacgtacc gccgggcacc
    13861 atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca cacgcaggct gttggtgatt
    13921 atccgcaaat gattatggtt aagtaacgcc cgggcaacat gctcaacagt cgtaccaatg
    13981 gtgataaata ttgttgaacc atcaggaata tagtctgcca ccgcttcggc aatcgctttt
    14041 tttttctcgg tttgcgaaac ctcacgctgc tcgaacgccg tattaacgac gctggaagcc
    14101 cgacccgcgc caccgtgatg gcgcgtaatc aggccttgct cgcttaattt acgaatatcc
    14161 cggcggaccg tctgcgtgga gacatccagc agatttgcca gctcatcaat attcatatac
    14221 ccacgctcgg cgatcagcat gaggagttgg tcgtgccgcg ggttaccggt tagttcggta
    14281 aggctcatga aaattcctcg aaaaaccatc accctggcat tttatacaaa aagtgacaaa
    14341 aaagatcggg tttgatcaca gtcagggatc ccggctcgtc caccgggacg tgtgcatttt
    14401 aacgccgcta caccgctggc gaagcggact gactccgcta aatccccact tgttgccagc
    14461 gccaccgcca aagcgccgtg aaaaacatca cccgcacctg tggtatctac cacatcaact
    14521 ttgaaggccg gttgatgctg acgcccacca ttttccagcc agtcgcagcc tgcgctaccc
    14581 tgggtcacat agacatgacc atttgtgagc gtttgtgcct gttttagcgc actggccatc
    14641 tctttcacgc ccgttaagcg cgccagaccc ggttctgaaa aggccgcgtg atcgcttaat
    14701 gccaccagct cactgatatc ctgcggcgta atgtccccgt ccagaacggt catcacaccc
    14761 gcctgacggg ccagggtgaa ggctttttta acgccgtcgt gccagcgtac atctgccagc
    14821 acaacatccc actgagagaa atcaatttcc tccaaccact ctgcgtcagg cagcagatcc
    14881 gggctggggt agttaatgat tatccgctcg cctttggtat ccaccatgat ggcggattgc
    14941 gaagatttcg cctggttata ccgtttggtg taacgggtgt taacgcccca ggattccagc
    15001 tctgctagca ggctgttgcc cgcgtcgtca tcacctacgc gaccaataaa atcgacctgc
    15061 gcccccagcc ttgccgccgc aaccgctgcc gtcgccgctg gcccgccacc aacttccgta
    15121 taatttctcg ccacgtattt accgctctcc gtcggtaacc cttccacgta atagatgcga
    15181 tccatcacgg ttatacctac acaagcaaca cgaatcatgg tcattcctta aacattttgg
    15241 gatgcgttca ttttcatttc accaattgtt taaaaagtga cggctgtcaa ttttttgacg
    15301 ataaattaca aatgcgatca aaaacagaca agcaaagcat gtcataaatg acaaaaagtg
    15361 acatcactgt attcaggaga gggttatggc agcaatcgcg tttatcggtt taggacaaat
    15421 gggttcgcca atggcgagca atttattgca gcaagggcat caacttcgcg tctttgatgt
    15481 gaacgccgaa gctgtgcggc atctggtaga caaaggtgcg actcccgccg ccaacccggt
    15541 gcaggccgct aaagatgccg aatttatcat taccatgttg ccgaatggcg atctggtgcg
    15601 caacgtgttg ttcggtgaaa acggcgtttg cgaaggctta tctaccgatg cgctggtcat
    15661 tgatatgtcc accatccatc cgctgcaaac cgataaattg attgccgata tgcaagccaa
    15721 aggcttcagc atgatggatg ttccggtagg ccgtacttct gcaaatgcca ttaccggtac
    15781 tctgttactg ctggctggcg gcaccgctga acaagttgaa cgtgccacac cgatcctgat
    15841 ggcgatgggc agtgagttga tcaacgctgg cggtccgggc atggggatcc gcgttaagct
    15901 catcaacaac tacatgagca tcgcgctcaa tgcgctttcg gcagaagccg ccgttttgtg
    15961 cgaagccctg aatcttccct tcgatgttgc cgtcaaagtg atgagcggta ccgccgccgg
    16021 taaaggtcac ttcaccactt cctggccaaa caaagtcctc agcggagatc tttctcccgc
    16081 cttcatgatc gatcttgccc ataaggatct tggcatcgcc cttgatgtcg ccaaccagct
    16141 gcatgtgcca atgccgctgg gggccgcctc acgggaggtt tatagccagg cgcgcgcatc
    16201 gggcggtttt gccatgctgg cggtcgatca gcgcgaagcc atgcgcatga tgtttgccgc
    16261 ggctggggca cccgctccgg tagccgatag cgttttaact gatttcaaag ttaacgctgc
    16321 aaaggccctc tcgccttatg cctcggcgat tctggtagat caacaattct gctaccgcca
    16381 ggtggttgag caaaacgcga ttgccaaaag ttgcgccatg attgtcgccg ccgatgagtt
    16441 cattcctggc aacggtattc cggtcgatag cgtggttatt gaccgcaaaa tcaatccgct
    16501 acagatcaaa caagacggtg gcaaagcctt aaaactgctg gtgctgtggc gtagtgatga
    16561 agatgcgcag caacgtctgg atatggtgaa agagttcaac ggactgtgcc actcacacgg
    16621 tctggtaagc atcattgagc cagtcgtccg cccaccgcgt cgtggcgata aattcgatcg
    16681 cgaacaagcg gtcatcgatg ccgccaaaga gctgggcgac agtggcgctg acctctacaa
    16741 agttgaaatg ccgctttatg gcaaaggtcc gcaacaagag cttctctgtg cttcacaacg
    16801 tctgaatgac catatcaata tgccatgggt gatcctctct tccagtgtcg acgaaaaact
    16861 gttcccgcgt gccgtacgcg tggcaatgac ggcgggcgca tcgggattcc tggcaggccg
    16921 tgcagtctgg gcatcggtcg tcggtttacc agacaacgag ctgatgctcc gtgacgtttg
    16981 cgcaccgaaa ttacaacaac ttggcgatat cgtcgacgaa atgatggcta aacgccgcta
    17041 agatgaggat aaaaggaatg aaatggttta acaccctaag ccacaaccgt tggctggaac
    17101 aggaaaccga ccgcatcttt gattttggta aaaattccgt agtgccgact ggttttggct
    17161 ggttaggcaa taaagggcaa atcaaagaag agatgggcac ccatctgtgg atcacagctc
    17221 gtatgttgca cgtttattcc gttgctgcgg cgatgggtcg acctggcgct tacgcgttgg
    17281 ttgatcacgg tattaaagcc atgaacggcg cactgcgtga taaaaaatat ggcggctggt
    17341 atgcctgcgt gaatgacgag ggcgtggtgg atgcctccaa acagggctat caacacttct
    17401 ttgctctgct gggtgccgcc agcgccgtca caacgggtca cccggaagcg cgcaagctgc
    17461 tcgattacac cattgagatt atcgagaaat atttctggag cgaagaagag cagatgtgcc
    17521 tggaatcctg ggacgaagcc ttcagcaaaa ccgaagagta ccgcggcggc aatgccaata
    17581 tgcacgcggt ggaagctttc ttgattgttt atgacgtcac tcacgacaaa aaatggctgg
    17641 atcgcgcgat tcgcgtggct tccgtgatta tccacgacgt cgccagaaat aatcattatc
    17701 gcgttaacga acacttcgat acccagtggg atccgctgcc ggattacaac aaagataacc
    17761 cggcgcatcg cttccgcgcg ttcggaggta caccaggcca ctggatcgaa tggggccgtt
    17821 taatgctgca catccatgct gccctggaag cccgttgcga acaaccacca gcatggctgc
    17881 tagaagatgc caaaggtctg tttaacgcca ccgtgcgcga tgcctgggca cccgatggcg
    17941 cggacgggat tgtttatacc gttgactggg aaggaaaacc ggtggtccgc gaacgtgtac
    18001 gttggcctat cgtcgaagca atgggtacgg cctacgcgct ctacaccgtc actggcgatc
    18061 gccagtatga aacctggtat caaacatggt gggagtactg cattaagtac ctgatggact
    18121 atgaaaatgg ttcctggtgg caggagctgg atgcggacaa taaggtcatc accaaagtct
    18181 gggacggcaa acaggatatt tatcacctgc tgcattgcct ggtgatcccg cgtatcccgt
    18241 tagcgcccgg catggctcca gcggttgcag cgggtctgct ggatattaat gcgaaataac
    18301 tgatctgaaa atagggtgcg ggttttcccc tcaccctaac cctctcccca aaggggcgag
    18361 gggaccgtcc ggtgcagttt ttgggtttca catcagcctc acgggaggct gtatgtcgtt
    18421 aattaaggtg ccttgtatgc aaatcaccaa tatgcattgc agtggacaga ccgtttcact
    18481 tgccgcgggg gattatcacg cgaccattgt gaccgtcggt gctggtctgg ctgaattaac
    18541 ctttcagggt tgtcatttag tcatcccgca caaacccgaa gagatgccgc tggcgcatct
    18601 gggtaaagtg cttattccgt ggccgaaccg gatcgccaac ggctgttacc gttatcaggg
    18661 gcaggaatat cagttaccca ttaatgaaca tagttcaaaa gccgctattc acgggctgct
    18721 tgcctggcga gactggcaaa ttagtgaatt aaccaccacc agcgttaccc tgacagcttt
    18781 tctgccgccc agttacggct atccgtttat gctggcttca caagttgttt attcgctcaa
    18841 cgcacacacg ggtctttccg ttgagatcgc aagccagaat atcggcactg tcgcagcgcc
    18901 ttatggcgtg ggtattcatc cttatttaac ctgcaactta acatctgttg atgaatatct
    18961 gttccagctt ccggcaaacc aggtttatgc cgtcgatgaa cacgtaaatc cgacaacgct
    19021 tcaccacgtt gatgagctgg atttaaattt cacgcaggcg aaaaaaatag ccgcgacaaa
    19081 aatcgaccac acctttaaaa ccgctaatga tttgtgggaa atgaccatta cccatccaca
    19141 gcaggcgctt tcggtaagcc tttgttccga tcaaccctgg gtgcaggttt atagcggtga
    19201 aaaattacag cgtcagggac tggcagttga acctatgagt tgcccgccta acgcctttaa
    19261 ttcaggtatc gacttattat tactggaacc aggaaaaccg caccgcctgt tttttaatat
    19321 ttacggacag agaaaataac ttgtattgat tagcagtcaa aatcattgaa gaacatttca
    19381 ctcgctaaag gcaacattat gtatcttcaa tttattaagt taaaaacagc tcagcttctg
    19441 taaagttttt atatatcagt tttatgagaa tcattttaca taagcctgta ataacaggaa
    19501 ggcaacaatc tcgttctcaa ctctatggag attagttatg gatacgccac gtccacagtt
    19561 attagatttt caatttcatc agaataacga cagttttacc ctacattttc aacaacgtct
    19621 tattttaacc catagcaaag ataatccttg tttatggatt ggctcaggta tagcggatat
    19681 cgatatgttc cgcggtaatt tcagcattaa agataaacta caggagaaaa ttgcgcttac
    19741 cgacgccatc gtcagccagt caccggatgg ttggttaatt catttcagcc gtggttctga
    19801 cattagcgcc acgctgaata tctctgccga cgatcagggc cgtttattgc tggaactaca
    19861 aaacgacaac cttaaccaca accgtatctg gctgcgcctt gccgctcaac cagaggacca
    19921 tatctacggc tgcggcgaac agttttccta cttcgatctg cgcggcaaac cgttcccgct
    19981 atggaccagt gaacaaggcg ttggtcgcaa caaacaaacc tatgtcacct ggcaggccga
    20041 ctgcaaagaa aatgcgggcg gcgactatta ctggactttc ttcccacagc ctacgtttgt
    20101 cagcacgcag aagtattact gccatgttga taacagttgc tacatgaact tcgactttag
    20161 cgccccggaa taccatgaac tggcgctgtg ggaagacaaa gctacgctac gttttgaatg
    20221 tgctgacaca tacatctccc tgctggaaaa attaactgcc ctgctgggac gccagccaga
    20281 actgcccgac tggatttatg acggggtaac gctcggcatt catggcggga cggaagtgtg
    20341 ccagaagaaa ctggacacca tgcgtaacgc gggcgtgaag gttaacggca tctgggcgca
    20401 ggactggtct gggatccgta taacctcttt tggcaagcgc gtgatgtgga actggaagtg
    20461 gaacagcgaa aactacccgc aactggattc acgcattaag cagtggaatc aggagggcgt
    20521 gcagttcctg gcctatatca acccgtatgt tgccagcgat aaagatctct gcgaagaagc
    20581 ggcacaacac ggctatctag caaaagatgc ctctggcggt gactatctgg tggagtttgg
    20641 cgagttttac ggcggcgttg tcgatctcac taatccagaa gcctacgcct ggttcaagga
    20701 agtgatcaaa aagaacatga ttgaactcgg ctgcggcggc tggatggctg acttcggcga
    20761 gtatctgccc accgacacgt acctgcataa cggcgtcagt gccgaaatta tgcataacgc
    20821 ctagcctgcg ctgtgggcga agtgtaacta cgaagccctt gaagaaacag gcaagctcgg
    20881 cgagatcctc ttctttatgc gcgccggttc taccggtagc cagaaatact ccaccatgat
    20941 gtgggcgggc gaccagaacg tggactggag tctcgacgat ggcctggcgt ccgtagtccc
    21001 ggcggcgctg tcgctggcaa tgaccggaca tggtctgcat cacagcgaca ttggcggtta
    21061 caccaccctg tttgagatga agcgcagcaa agagctgctg ctgcgctggt gcgatttcag
    21121 cgccttcacg ccgatgatgc gcacccacga aggtaaccgt cctggcgaca actggcagtt
    21181 tgacggcgat gcggaaacta tcgcccattt tgcccgtatg actaccgttt tcaccaccct
    21241 gaaaccttat ctgaaagagg ccgtcgcgct gaatgcgaag tccggtctgc cggtcatgcg
    21301 cccgctgttc ctgcattacg aagacgatgc acatacctat tccctgaaat atcagtacct
    21361 gttaggccgc gacattctgg tcgctccggt acacgaagaa ggtcgcagcg actggacgct
    21421 ctatctgccg gaggataact gggtacatgc ctggacgggt gaagcgttcc ggggcgggga
    21481 agttaccgtt aatgcgccca tcggcaagcc gccggtcttt tatcgcgccg atagcgaatg
    21541 ggcggcactg ttcgcgtcgt taaacagcat ctaatcacat ccgcccgtgg ggaaactcat
    21601 gggcaaaggg agaacatcga tgagtcacat cacaacggaa gatccagcaa ctttacgcct
    21661 gccctttaaa gagaaactct cttacggtat tggcgacctg gcctctaaca tcctgctgga
    21721 tatcggtacg ctttatcttt tgaagtttta taccgacgtt ctggggctgc caggcaccta
    21781 tggcggcatt atctttttga tttcaaaatt ctttactgcg tttaccgata tgggtaccgg
    21841 cattatgttg gattcccgac gcaagatcgg tccaaaaggt aagttccgtc cttttattct
    21901 gtatgcgtca ttcccggtca ccttactggc gatcgccaac tttgtcggca caccgtttga
    21961 tgtcaccggt aaaacggtga tggccactat tctgtttatg ctttacggac tgtttttcag
    22021 catgatgaac tgctcctacg gcgcaatggt cccggcaatc accaaaaacc ccaacgaacg
    22081 cgcctcactg gcggcatggc gtcagggcgg cgctacgctg ggcctgctgc tgtgcacggt
    22141 gggattcgtg ccagttatga atcttatcga aggtaatcag aaacttggct atatcttcgc
    22201 cgccacgctg ttttcactgt tcggcctgct gtttatgtgg atctgctact caggcgtgaa
    22261 agagcgttat gtcgaaaccc aaccaaccaa tccggcgcaa aagcctggcc tgttgcaatc
    22321 tttccgcgca attgccggta accgcccact gttcattctg tgtattgcca acctctgcac
    22381 cttaggggcg tttaacgtca agctcgccat tcaggtctat tacacccagt acgtacttaa
    22441 cgatcccatc ctgttgtcgt atatgggatt tttcagcatg ggctgtattt tcatcggcgt
    22501 gttcctgatg cctggcgcag tcagacgttt tggtaagaag aaggtctata tcggcggcct
    22561 gctgatttgg gtgctgggcg atctgctcaa ctatttcttc ggcggcggct cggtcagctt
    22621 tgtggcgttc tcctgcctgg cgttcttcgg ctcagcgttt gttaacagcc tgaactgggc
    22681 gctggtttcc gacaccgtcg agtacggcga gtggcgcacc ggcgtgcgtt cggaaggcac
    22741 ggtctacacc ggcttcacct tctttcgcaa agtgtctcag gcgctggctg gtttcttccc
    22801 cggctggatg ctgacgcaaa ttggctatgt gccgaacgtc gcacaggctg accacaccat
    22861 tgaagggtta cgccagttga tcttcatcta cccaagcgca ctggcggtag tcaccattgt
    22921 ggcgatgggt tgcttctaca gcctgaacga gaagatgtat gtccgcattg tggaagagat
    22981 agaagcccgt aaacgcacgg cgtaataata ataaacgacg ccctgcgggg cgttataagg
    23041 agtgattatg tctgaccata atccactgac attaaaactg aatctgcggg aaaaaatcgc
    23101 ctatggtatg ggcgacgtcg gttcgaattt aatgctctgc atcggtactc tgtatctcct
    23161 caaactttat accgatgaat tagggatgcc tgcttactat ggcgggatta tctttctggt
    23221 cgcgaagttt tttaccgcgt ttaccgatat gctcaccgga tttttactcg actcgcggaa
    23281 aaatattggg cctaaaggca aatttcgtcc ctttatttta tatgcagcag ttccggcggc
    23341 gttaattgca acgctccagt ttatcgccac caccttttgt ttgccggtta aaacgacgat
    23401 tgccaccgcg ctgtttatga tgtttggact ttcatacagt ctgatgaact gctcgtatgg
    23461 tgcgatgatc ccggcaatta ccaaaaaccc gaatgagcgc gcgcagctcg cggcttaccg
    23521 ccagggtggt gcaaccatag ggctattgat ttgtaccgtg gcgtttattc cgttgcagtc
    23581 gcttttttct gactcaaccg tcggttatgc ctgtgcggca cttatgttct ccattggcgg
    23641 ctttattttt atgatgctgt gctacagagg cgtcaaagag cattatgtgg acacagcgcc
    23701 aaccggacat aaagccagta ttctcaaatc tttttgcgcg atatttcgta atccaccatt
    23761 gctggtttta tgcattgcta acctgtgtac tctggcggca tttaatatca aactggcgat
    23821 tcaggtctat tacacccagt atgtgctgaa tgatattaat ttattgtcgt ggatgggatt
    23881 cttcagtatg ggatgcatcc tcgtcggcgt attactggtg ccagtaactg taaaatgttt
    23941 tggtaaaaaa caggtttatt tagctggcat ggtgctatgg gcggtgggtg atatactgaa
    24001 ttatttctgg ggaagtaact ctttcacttt cgtcatgttc tcttgtgtcg ccttttttgg
    24061 cacggccttt gtaaacagcc tgaactgggc actggtacca gataccgtag attacggtga
    24121 atggaaaacc ggtattcgtg ccgaaggttc tgtttatacc ggttatacct tctttcgtaa
    24181 aatttctgcc gcacttgctg gcttcttgcc aggcattatg ctgacgcaaa ttggttatgt
    24241 tcccaacatc gcgcaaagcg atgcgacatt acagggtttg cgtcagctca tttttatctg
    24301 gccttgtgca ctggcaatta ttgctgcatt gacgatggga ttcttttaca cactcaatga
    24361 aaaacggttt gcattgatta ttgaggaaat caaccaacga aaaaataaag agatggcgac
    24421 agaaaaaaaa acggcttccg taacgttata aatgtcaata gccgctattt ccatcctggt
    24481 gggtggcggc ctccctacgt ttaaaaaatg gacttattat gaaaaagatt aatgcaataa
    24541 ttttattatc ttctctgacc tctgcctcgg tatttgctgg cgcatacgtc gaaaacaggg
    24601 aggcatataa tcttgcctcc gatcagggcg aggttatgct acgtgtgggt tataacttcg
    24661 atatgggcgc gggtattatg ttaaccaata cctacacctt tcagcgagaa gatgaactaa
    24721 aacatggcta taatgaaatt gaaggttggt atcctttatt caaaccaacc gataaattaa
    24781 ccatccagcc cggtggctta atta
//
LOCUS       DAJTYF010000050        24451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTYF010000050 DAJTYF010000000
VERSION     DAJTYF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.050"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..487)
                     /locus_tag="OO590_002158"
     CDS             complement(<1..487)
                     /locus_tag="OO590_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6637521.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LL"
     gene            760..948
                     /locus_tag="OO590_002159"
     CDS             760..948
                     /locus_tag="OO590_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6637522.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            960..1670
                     /gene="bcsQ"
                     /locus_tag="OO590_002160"
     CDS             960..1670
                     /gene="bcsQ"
                     /locus_tag="OO590_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6637523.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1667..4280
                     /gene="bcsA"
                     /locus_tag="OO590_002161"
                     /pseudo
     CDS             1667..4280
                     /gene="bcsA"
                     /locus_tag="OO590_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4291..6630
                     /gene="bcsB"
                     /locus_tag="OO590_002162"
     CDS             4291..6630
                     /gene="bcsB"
                     /locus_tag="OO590_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR6637524.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6637..7743
                     /gene="bcsZ"
                     /locus_tag="OO590_002163"
     CDS             6637..7743
                     /gene="bcsZ"
                     /locus_tag="OO590_002163"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6637525.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7802..7978
                     /locus_tag="OO590_002164"
                     /pseudo
     CDS             7802..7978
                     /locus_tag="OO590_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8306..10054
                     /gene="bcsC"
                     /locus_tag="OO590_002165"
                     /pseudo
     CDS             8306..10054
                     /gene="bcsC"
                     /locus_tag="OO590_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10136..12124
                     /gene="hmsP"
                     /locus_tag="OO590_002166"
     CDS             10136..12124
                     /gene="hmsP"
                     /locus_tag="OO590_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6637526.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12308..13594
                     /gene="dctA"
                     /locus_tag="OO590_002167"
     CDS             12308..13594
                     /gene="dctA"
                     /locus_tag="OO590_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6637527.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFSSGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13815..15311
                     /locus_tag="OO590_002168"
     CDS             13815..15311
                     /locus_tag="OO590_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6637528.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15407..16336)
                     /gene="kdgK"
                     /locus_tag="OO590_002169"
     CDS             complement(15407..16336)
                     /gene="kdgK"
                     /locus_tag="OO590_002169"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6637529.1"
                     /translation="MSKKIAVIGECMIELSEKDADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16568..17335
                     /gene="pdeH"
                     /locus_tag="OO590_002170"
     CDS             16568..17335
                     /gene="pdeH"
                     /locus_tag="OO590_002170"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6637530.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17405..19465
                     /locus_tag="OO590_002171"
     CDS             17405..19465
                     /locus_tag="OO590_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6637531.1"
                     /translation="MSKAGKITAAISGVFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19647..20969)
                     /locus_tag="OO590_002172"
     CDS             complement(19647..20969)
                     /locus_tag="OO590_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6637532.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21380..22393)
                     /gene="yhjD"
                     /locus_tag="OO590_002173"
     CDS             complement(21380..22393)
                     /gene="yhjD"
                     /locus_tag="OO590_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6637533.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22442..23341)
                     /locus_tag="OO590_002174"
     CDS             complement(22442..23341)
                     /locus_tag="OO590_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6637534.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            23861..>24451
                     /locus_tag="OO590_002175"
     CDS             23861..>24451
                     /locus_tag="OO590_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6637535.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLN"
BASE COUNT         5786 a         6154 c         6733 g         5778 t
ORIGIN      
        1 caagcaacaa tgaaaattgt ttatcgttat tatttccggg attaattacc aaaagcgaac
       61 aatggtttaa cctgctccat ttattcattt tatccaacca tgagcgaagc cgctcggcag
      121 gaatgttttg ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta tggggatcaa
      181 tagaacactg caaatcacgg gccaaatagt atagaccttt ttcatgattt agcattgaaa
      241 ataattttat tttttccggc ccttgagaat catctaattg aaagattttc gccggatcgc
      301 tgtccatgct aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt gtttgattcg
      361 ccagactgat agcatcttca tggcgatcga cgttaaacca ccagacgccg cctgctggca
      421 tatgtcgcag ctcatcccat aatgatgaga taccgataga gaatacaggg tccacaatgt
      481 ccctcatgtc accattttgt ctgattatca gtttactagc gaaagctaag aaataaacct
      541 accattgaaa ttaaaactta tcagatttag catgtgaatc aatttagaag gtcacaagac
      601 gtgtgatttt agtcttttcg tttttaactt tttctgttgc gtaacggaaa gtcaaaaagt
      661 gagcaaattc ccgtctcgcc gtaaacaata aaccggcgaa atgctcacgg ttagaattgt
      721 atcatgaatg gtacggttat ttcataggga tcaagcaaaa tgaataacaa tgaaccagat
      781 actctgcctg atcccgcgat aggctatatc ttccagaatg atattgtggc gttaaagcag
      841 gcattttcac tgcctgatat tgattatgcc gatatttccc aacgcgaaca gttggccgcg
      901 gcattaaaac gctggccgtt gctggcagag tttgcgcaac aaaagtaggg gattggtgaa
      961 tggccgtact gggattgcag ggggtgcggg gaggcgtggg gacaacaacc atcaccgccg
     1021 cattagcctg gtcattacaa atgttgggag aaaatgtcct agtggtcgat gcctgcccgg
     1081 acaacttgtt gcgcctgtca tttaatgttg attttaccca ccgtcagggc tgggccagag
     1141 cgatgctgga tggccaggac tggcgtgacg ctgggttgcg ctacacctcg cagctcgatt
     1201 tgctgccttt tggtcagtta tccattgaag aacaagaaaa tccacagcac tggcaaaccc
     1261 ggctgagcga tatttgctcc ggcttacagc aactaaaagc cagcgggcgt taccagtgga
     1321 ttttaatcga cttaccgcgt gatgcctcgc agataaccca ccagctgctg agtttgtgcg
     1381 agcactcgct ggcaatcgtc aatgtggatg ccaactgcca tatccgactg catcagcaag
     1441 cgctgccgga tggcgcacat attttgatta atgacttccg tattggcagt caggttcagg
     1501 acgatattta ccagctttgg ttgcaaagcc agcgccgatt actgccgatg ctcattcatc
     1561 gtgatgaagc gatggctgaa tgcctggcgg ctgaagagat actgacgctg gcgaactggt
     1621 gcctgttgaa ctactccggg ctgaaaacgc cagtcgggag tgcatcatga gtatcctgac
     1681 ccggtggttg cttatcccgc cggtcaacgc gcggcttatc gggcgttatc gcgattatcg
     1741 tcgtcacggt gcgtcggctt tcagcgcgac gctcggctgt ttctggatga tcctggcctg
     1801 gatttttatt ccgctggagc acccgcgctg gcaacgtatt cgcgcagaac ataaaaacct
     1861 gtatacgcca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
     1921 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
     1981 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     2041 ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg
     2101 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     2161 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
     2221 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
     2281 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
     2341 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc
     2401 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
     2461 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
     2521 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
     2581 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     2641 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
     2701 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
     2761 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
     2821 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
     2881 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
     2941 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
     3001 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
     3061 gtggctatac ctccgtgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
     3121 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtacaa atcttccgtc
     3181 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
     3241 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     3301 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
     3361 tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact
     3421 ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg
     3481 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     3541 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc
     3601 tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca
     3661 ccgtggtcgt cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg
     3721 cggtatcggt agaaagcaaa caggtacgcc gatcgcaccg cgtggagatg acgatgcccg
     3781 cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg
     3841 gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc
     3901 tgttgcttaa acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg
     3961 gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc
     4021 agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata
     4081 agccgctgga aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg
     4141 cggagtttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct
     4201 gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc
     4261 aggctttggc tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca
     4321 gtggctatgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa
     4381 ccactgatca atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg
     4441 caagtgatgt caggcgagca gggagctgat gcccccatcg tggcgcagaa cggtccttcg
     4501 cgtgatgtga agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt
     4561 ggtattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag
     4621 gcgatgctca acctcgaata cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta
     4681 aaggtttatc tcaatgatga gctgatgggc gtgctgccag tgaccaaaga acagttgggt
     4741 aaaaaaacgc tggcgcaaat gcccattaat ccactgttta ttaccgactt caaccgtgtg
     4801 cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt
     4861 tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag
     4921 aatgacctgt cacacttccc ggtgccattc tttgacccgc gcgataaccg tactaacacc
     4981 ttgccgatgg tctttgcggg tgcgccggat gttgggctgc aacaagcatc tgccattgtc
     5041 gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac
     5101 caattgccgg atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc ggacttcctg
     5161 cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct
     5221 tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa
     5281 ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa
     5341 ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc
     5401 acatttggcg aactgaaaac ctatgaagaa cagttacaat ccagcggcct tgagccagca
     5461 gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt
     5521 gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat
     5581 atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac
     5641 cgtctgctgc tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac agatgtctct
     5701 attccggcgc tgaaactggg cgcgaccaac cagctgcgct tcgactttga gtatatgaac
     5761 ccgatgccag gcggttcggt agataactgt attaccttcc agccggtgca gaatcatgtg
     5821 gtgattggtg acgattccac catcgacttc tcgaagtatt accacttcat tccgatgccg
     5881 gatctacgcg cctttgctaa cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa
     5941 accatcaccg tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt gctgaatact
     6001 gttggtttta tcggcgcaca gacgggcttc ccggcgatta atctgacggt gaccgatgat
     6061 ggcagcacca ttcagggcaa agatgccgac atcatgatca tcggtggtat cccggacaaa
     6121 ctgaaagacg ataagcagat cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca
     6181 ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg agagcgatcg cgcggcagaa
     6241 acccagtcaa cgctgacctc ttccggtgcg atggcggcgg tgattggctt ccagtcgccg
     6301 tataacgacc agcgcagcgt gattgcgctg ttggcagata gcccacgcgg ttatgaaatg
     6361 cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg
     6421 atccgcgagt acggtatcaa cagcctacgt gttggcgacg tttattacgt aggtcatctg
     6481 ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg
     6541 gcggctatca gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg aattattagt
     6601 cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggaatc
     6661 gtgacgatgc tgttgttggc tgcctttagt gttcaggcag cctgtacctg gcctgcctgg
     6721 gagcagttta aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg
     6781 cgcaaaatca ccacctccga agggcaaagt tacggtatgt tctttgccct ggcggctaac
     6841 gaccgtgcag ctttcgataa tattctcgac tggacgcaga acaatctcgc tcagggttct
     6901 ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg
     6961 ctggacagca attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg
     7021 gggcgtttgt ggaaagagca gcgttatacc gacatcggca gcgcgttgct aaaacgtatc
     7081 gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg
     7141 ggttttgctg aggataacag ctggcgtttt aaccccagct acatgccgcc gacgctggcg
     7201 cagtatttca cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacattta
     7261 ttgctggaaa ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa
     7321 ggctggcagc taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac
     7381 atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt
     7441 aaaccgatgg cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct
     7501 acggggaaag cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcctttttta
     7561 caaaaccgcg atgcgcaggc agttcagcgc cagcgcgtgg ccgataactt tcccggcagc
     7621 gatacctatt acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc
     7681 cgcttctcga caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac
     7741 taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa
     7801 ccatcagcag accctgcgga tggacagcgg caacactaat gccgtgcgcg ggctggcgaa
     7861 tatttaccgc cagcagtcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag
     7921 ccagcggcgc agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
     7981 ggcagaggta ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc agcggcaacg
     8041 actggcgctg gaccccggca gcgtatggat tacttaccga ctttcgcagg atctctggca
     8101 ggccggacaa cgcagccagg ccgatacgtt aatgcgcaat ctggcgcagc agaagccgaa
     8161 caacccggag caggtttacg cttacgggct gtacctctca ggtcacaacc aggacagagc
     8221 ggcgctggcg catatcaaca gcctgccgcg cgcgcagtgg aacagcaata ttcaggagct
     8281 ggttaatcga ctgcaaagcg atcaggtgct ggaaaccgct aatcgcctgc gagaaagcgg
     8341 caaagaggct gaagcggaag cgatgctgcg tcagcaacca ccttccacgc gtattgacct
     8401 cacgctggct gactgggcgt aacaacgacg tgattacacc gccgcccgca ctgcatatca
     8461 gaatgtcctg acgcgggagc caactaacgc cgatgccatt cttggtctga cggaagtgga
     8521 tattgctgcc ggtgacaaag cggcggcacg tagccagctg gcgaaactgc ctgccaccga
     8581 taacacctcg ctgaacactc agcggcgcgt ggcgctggca caggcgcagc ttggagatac
     8641 cgcagcggcg cagcagacgt ttaataagtt gatcccgcag gcaaaatctc agccaccgtc
     8701 gatggaaagc gcgatggtgc tgcgcgacgg tgcgaagttt gaagcgcagg cgggcgatcc
     8761 aaagcaggcg ctggaaacct acaaagacgc catggtcgca tccggtgtga ctacgacgcg
     8821 tccgcaggat aacgacacct ttacccgact gacccgtaac gacgagaaag atgactggct
     8881 gaaacgcggc gtgcgcagcg atgcggcgga cctctatcgc cagcaggatc ttaacgtcac
     8941 ccttgagcac gattactggg gttcgagcgg caccggtggt tactccgatc tgaaagcgca
     9001 cactaccatg ttgcaggtgg atgcgccgta ttctgacggg cggatgttct ttcgcagtga
     9061 tttcgtcaat atgaacgtcg gcagtttctc cactaatgcc gatggcaaat gggatgacaa
     9121 ctggggcacc tgtacattac aggactgtag cggcaaccgc agccagtcgg attccggtgc
     9181 cagcgtggcg gtcggctggc gaaatgacgt ttggagctgg gatatcggta ccacgccgat
     9241 gggcttcaac gtggtggatg tggtcggcgg catcagttac agcgatgata tcgggccgct
     9301 gggttacacc gttaacgccc accgtcggcc catctccagt tctttgctgg cctttggtgg
     9361 gcaaaaagac tccccgagca ataccgggaa aaaatggggt ggcgtacgtg ccgacggtgt
     9421 ggggctaagt ctgagctacg ataaaggtga agtaaacggc gtctgggcat cgcttagtgg
     9481 cgaccagtta accggtaaaa atgtcgaaga taactggcgc gtgcgctgga tgacgggcta
     9541 ttactataag gtcattaacc agaacaatcg ccgcgtcaca atcggcctga acaacatgat
     9601 ctggcattac gacaaagatc tgagtggcta ctcactcggt cagggcggtt actacagtcc
     9661 gcaggaatac ctgtcgtttg ccataccggt gatgtggcgg gagcgcacgg aaaactggtc
     9721 gtgggagctg ggggcgtctg gctcgtggtc gcattcacgc accaaaacca tgccgcgtta
     9781 tccgctgatg aatctgatcc cgaccgactg gcaggaagaa gctgcgcggc aatccaacga
     9841 tggcggcagc agtcagggct tcggctacac ggcgcgggca ttacttgaac gacgtgttac
     9901 ttccaactgg tttgttggca cggcaattga tatccagcag gcgaaagatt acgcacccag
     9961 ccatttcctg ctctacgtac gttattccgc cgccggatgg cagggtgaca tggatttacc
    10021 gccgcagccg ctgatacctt acgccgactg gtaagttttc agatagcgcc tctcttaatg
    10081 ccgctgcgat cgggtatact cgggcggcaa tctgggattt ccggggggag acaatttgcg
    10141 cgtaagtcgc tcgttaacaa tcaagcagat ggcaatggtg gcagccgttg tcctggtgtt
    10201 cgtttttatt ttttgcaccg ttttgctgtt ccatctggtc cagcagaatc gctataacac
    10261 ggctacgcaa ctggaaagca ttgctcgctc tgtccgcgaa cccttatctt cagctatttt
    10321 gaaaggcgat attcccgaag cggaagctat tcttgccagc attaaaccgg caggcgtggt
    10381 cagccgtgcc gatgtagtgc tgcctaacca gttccaggcg ctgcgtaaaa gttttattcc
    10441 agagcgtccg gtgccggtaa tggttactcg cctgtttgag ctaccggttc aaatctcgct
    10501 gggcgtctac tcgctcgaac gtccggcaaa tccgcagcca atagcctatc tggtgctaca
    10561 ggcggattcc ttccgtatgt ataagttcgt gatgagcact ctctcaacgt tagtgaccat
    10621 ttacttactt ttgtcgctta tcctgaccgt cgccatcagc tggtgcatta accgcctgat
    10681 tttgcatccg ttacgcaata ttgctcgcga acttaacgcc atcccagccc aggagcttgt
    10741 tggtcaccaa ctggcattac cgcgtctgca tcaggacgat gaaatcggta tgttggtgcg
    10801 cagttacaac ctcaaccagc aattgctgca gcgccattat gaagaacaga acgaaaatgc
    10861 gatgcgcttc ccggtgtcgg atttgccgaa caaagccttg ctgatggaga tgctggagca
    10921 ggttgtcgcg cgtaaacaaa ccaccgcgct gatgatcatc acctgtgaaa ccctgcgtga
    10981 tactgcgggc gtgctgaaag aggcgcaacg agaaattctg ctgctgacgc tggtggaaaa
    11041 actcaaatcg gtactgtcgc cacgtatgat cctcgcgcag attagcggtt atgactttgc
    11101 tgtcattgcc aacggtgtac aggaaccgtg gcacgcaatc actttgggtc agcaagtact
    11161 cactatcatg agcgagcgcc tgccgattga acgtattcaa ctccgtccgc actgtagcat
    11221 tggcgtggcg atgttctacg gcgatctcac cgccgaacag ctttacagtc gcgctatttc
    11281 tgcggcattt accgctcgcc ataaaggcaa gaatcagatt cagttctttg atccgcagca
    11341 gatggaagca gcccagaagc ggttgacgga agagagcgat atccttaatg cactggaaaa
    11401 tcatcagttt gcaatttggt tacagccaca ggtcgagatg accagcggta aactggtcag
    11461 tgcggaagtg ttactgcgta tccagcaacc ggatggcagt tgggacctgc cggatggctt
    11521 aatcgatcgc attgagtgct gtgggctgat ggttaccgtc ggtcactggg tgctggaaga
    11581 gtcctgtcga ttgcttgcag cctggcaaga gcgcggcatt atgctgccct tgtcggtaaa
    11641 cctctctgcg ctgcaactga tgcacccgaa tatggtggcg gatatgctgg aactgttaac
    11701 ccgctatcgc attcagccgg gaacactgat tctggaagtg acagaaagcc gacgtattga
    11761 cgaccctcat gctgcggtgg caatcctccg tccgctgcgc aatgccggag ttcgggtggc
    11821 gctggatgat ttcggcatgg gctacgcagg gctgcgtcag ctgcagcata tgaaatcgtt
    11881 gccaatcgac gtactgaaaa tcgacaaaat gtttgttgaa ggcttgccgg aagatagcag
    11941 catgattgct gcaattatca tgctggcgca gagcctgaac ttacaaatga ttgccgaagg
    12001 cgtggagact gaagcacaac gagactggct ggcaaaagcg ggcgttggta ttgcccaggg
    12061 cttccttttt gctcgcccac tccctattga aatcttcgaa gagagttacc tggaagaaaa
    12121 gtagctaccc caaactgatt acaaaacttt aaaaagtgct ggtttgtgcg agccagctca
    12181 aactttttaa cctttttgtt tcaattatga tccaggtaca tttctgtgat gttgtctggg
    12241 tgttatttta aggccgcagg tacccccata accttacaag acctgtggtt ttactaaagg
    12301 acaccctatg aaaacctctc tgtttaaaag cctttacttt caggtcctga cagcgatagc
    12361 cattggtatt ctccttggcc atttctatcc tgaaataggc gagcaaatga aaccgcttgg
    12421 cgacggcttc gttaagctca ttaagatgat catcgctcct gtcatctttt gtaccgtcgt
    12481 aacgggcatt gcgggcatgg aaagcatgaa ggcggtcggt cgtaccggcg cagtcgcact
    12541 gctttacttt gaaattgtca gtaccatcgc gctgattatt ggtcttatca tcgttaacgt
    12601 cgtgcagcct ggtgccggaa tgaacgtcga tccggcaacg cttgatgcga aagcggtagc
    12661 ggtttacgcc gatcaggcga aagaccaggg cattgtcgcc ttcattatgg atgtcatccc
    12721 ggcgagcgtc attggcgcat tttccagcgg taacattctg caggtgctgc tgtttgccgt
    12781 actgtttggt tttgcgctcc accgtctggg cagcaaaggc caactgattt ttaacgtgat
    12841 cgaaagtttc tcgcaggtca tcttcggcat catcaatatg atcatgcgtc tggcacctat
    12901 tggtgcgttc ggggcaatgg cgtttaccat cggtaaatac ggcgtcggca cactggtgca
    12961 actggggcag ctgattatct gtttctacat tacctgtatc ctgtttgtgg tgctggtatt
    13021 gggttcaatc gctaaagcga ctggtttcag tatcttcaaa tttatccgct acatccgtga
    13081 agaactgctg attgtactgg ggacttcatc ttccgagtcg gcgctgccgc gtatgctcga
    13141 caagatggag aaactcggct gccgtaaatc ggtggtgggg ctggtcatcc cgacaggcta
    13201 ctcgtttaac cttgatggca catcgatata cctgacaatg gcggcggtgt ttatcgccca
    13261 ggccactaac agtcagatgg atatcgtcca ccaaatcacg ctgttaatcg tgttgctgct
    13321 ttcttctaaa ggggcggcag gggtaacggg tagtggcttt atcgtgctgg cggcgacgct
    13381 ttctgcggtg ggccatttgc cggtagcggg tctggcgctg atcctcggta tcgaccgctt
    13441 tatgtcagaa gctcgtgcgc taactaacct ggtcggtaac ggcgtagcga ccattgtcgt
    13501 tgctaagtgg gtgaaagaac tggaccacaa aaaactggac gatgtgctga ataatcgtgc
    13561 gccggatggc aaaacgcacg aattatcctc ttaatctcac cacttatgcc cgcactccct
    13621 tccccgagtg cgggtgtttg cgcataattg acccctgtgc ctgaacttac cacctgggtg
    13681 agttcaggtt tatttcactt cttccgtgac attttcatgt tcttgcggtc taacacgaag
    13741 tgtttttaac gtcatttaga ctgaccgaca acgggcagtt tttttattac caggatagtt
    13801 gatcaggggt tcacatgcag ggcataaaaa ttcgactttt agcgggcggt ttgctgatga
    13861 tggccactgc tggctatgtg caggcagatg cgctccagcc tgatccagca tggcaacagg
    13921 ggacgctttc caacggttta cagtggcaag tgctgaccac cccccagcgt cccagcgatc
    13981 gtgttgaaat tcgcctgctg gttaataccg gttcgctcgc cgaaagtaca caacagagcg
    14041 gttacagtca cgccatccct cgtattgcgc taacgcaaag cggtggcctt gacgcagcac
    14101 aggcgcgttc attgtggcag caggggatcg accctaaacg cccgatgccg ccggtaattg
    14161 tctcttatga caccacgctg tttaatctga gtttgcccaa taaccgtaac gatttgctga
    14221 aagaagcgct ctcttatctg gcaaatgcca ctggcaaatt gaccatcaca ccagaaacca
    14281 tcaaccacgc gctgcaaagt caggacatgg tggcaacctg gcctgccgat actaaagagg
    14341 gctggtggcg ctatcgtctg aaagggtcaa ccttgttagg tcacgatcct gccgatccgc
    14401 tgaaacaacc cgttgaagcg gaaaaaatta aagatttcta tcagaaatgg tacaccccgg
    14461 atgcaatgac gctactggtg gtgggaaacg tggatgcgcg ctcggttgtc gaccaaatca
    14521 acaaaacgtt tggcgaactg aaaggcaaac gtgaaacgcc agctccggtg ccgacgcttt
    14581 ctccgctgcg tgcggaagcg gtgagtatta tgactgacgc ggtgcgtcag gaccggttat
    14641 ctatcatgtg ggatacgccg tggcagccga ttcgtgaatc agccgcactg ctgcgctact
    14701 ggcgtgcgga cctggcccgc gaggcgctgt tctggcatgt tcagcaagcg ttaagcgcca
    14761 gtgacagcaa agacatcggt cttggatttg actgccgtgt gctgtatctg cgtgcgcagt
    14821 gtgccatcaa catcgaatca ccaaacgaca agctgaacag caaccttaat ctggtggcgc
    14881 gtgaactggc gaaggttcgc gataaaggtc tgccggaaga agagttcaat gcgttagtgg
    14941 cgcaaaagaa actggagctg cagaaactgt ttgccgccta tgcacgggct gataccgata
    15001 ttctgatggg ccagcggatg cgttcgttgc aaaatcaggt cgtcgatatc gcgccggagc
    15061 agtatcagaa actgcggcag gatttcctta acagcctgac tgtggagatg ttaaatcagg
    15121 atctgcgtca gcagttgtcg aatgatatgg cgttaatact gctgcagccg aaaggcgagc
    15181 cggaatttaa catgaaagcg ttgcaggcgg cctgggatca aatcatggcc ccatctaccg
    15241 ccgctgcgac cacctctgtc gccacggatg acgtacatcc tgaagtgacg gatattccac
    15301 ctgcacagta atgcctgatg gtgatgagtt agcacaacca tcaggtattt atggatgagc
    15361 tggatagagg ggtaacactt tatccagcct tttgcatatg ctgcgtttac gctggcatcg
    15421 cctcacgcgg gataatcgcg ccgcgatact gaataacggt acttgcggtc aggtgcccgc
    15481 gtttcgccgc gttttccgca ctgccgcccg tcaaacgtac cgccagataa ccggcgctga
    15541 aagagtcgcc agctgcggtg gtatcgatca ctttttcttt cggcagtttc accgccggaa
    15601 catcaactaa cgcttcacca gcaatggaca ccaggcaaga atccgccccg cgtttcacca
    15661 ccacttcttt cacgcccgcg ttatgggtgc gcgcaatgac gtcttccacc ggctgttgac
    15721 cccacagcgc gtcttcgtcg tccagcgtca ggaaggcgat atccgtgcat tccagcattt
    15781 gctggtacac ctgctgtgtc tcttctttgc tggcccacag gcgcggacga tagttattgt
    15841 cgaaaatcac ttttccgcct ttggcgcggc attcgcgcag cagggaaagc agcttttcgc
    15901 ggctggtcgg gcttaagatc gccaggctaa tcccgctcag gtagagataa tcgaaattcg
    15961 ccagctcttc gcaaatcgcc gcagactgct cgctcgccag ccagaatttg gcggcggctt
    16021 cgttacgcca gtagtagaac gtacgttcgc cggtgctgtc ggtttcaatg tagtaaaggc
    16081 ccggaagacg gttttccatc cgttgggtca gggaagtatc aacgttctcg ccgtgccagg
    16141 cgtccagcat ctgctggcta aaactgtccg ttcccagcgc cgttacgtaa tgaacggtta
    16201 atgccgcagg atcgacctga cgggcgatat agacggaagt gttcagggta tcgccgccga
    16261 aaccgcgctt aacgtccgcg tctttctcgg aaagctcaat catgcattcg ccaatcacgg
    16321 caatcttttt ggacatagtc gtgaacctga tctgtaaaaa attagcgtta gtttgcgctg
    16381 tggtggtttg ctggtcaacc atattaaaac agtgttccat tatttttttg agccagaacg
    16441 tattcctgaa agattccgtt gtggagtgag gaaatttacc ccggccaggc ggtaaagttc
    16501 tgcccttacg cgccgataat ctttgtcgag tccgggcagc atcactttta aacacaggac
    16561 atctttgatg ataaggcagg ttatccagcg aataagcaac cctgaagcaa gcatcgagag
    16621 cttgcaggaa cggcgttttt ggttgcagtg tgagcgtgct tacacctggc agccgatcta
    16681 tcaaacatgc gggcggttaa tggccgtgga gctattaacg gtggtcacgc atcccttgaa
    16741 cccttcgcaa cgcctgccgc cggatcgcta ttttactgaa atcaccgtca gccatcggat
    16801 ggaggttgtg aaagagcaga ttgatttgct ggcgcaaaaa gccgacttct ttgtagagca
    16861 cggcctgctg gcatcggtca atattgatgg ccctacgctc atcgccctgc gtcagcaacc
    16921 aaaaatcctg cgccagattg agcgtcttcc ctggctgcgt ttcgaactgg tggagcatat
    16981 ccgtctgccg aaagattcaa cctttgcctc gatgtgtgaa tttggcccgc tgtggctgga
    17041 tgattttggt accgggatgg caaatttctc tgcactaagt gaagtgcgtt atgactacat
    17101 caaaatcgcg cgagaactgt ttgtgatgct gcgtcagtcg ccggaaggcc gcacactctt
    17161 ttctcagctt ttacatctaa tgaatcgcta ttgtcgcggg gtgattgtcg agggcgtaga
    17221 aacaccggaa gagtggcgtg atgttcagaa ctcgcccgca ttcgccgcac aaggctggtt
    17281 tctttcacgc ccggcaccga tggaaacgct gaatacggcg gttctggcgc tataagctgc
    17341 ctcattttcc gccgggctgg actatcttta ggactggcac aggaagaata tgaggaagcg
    17401 aacgatgagc aaggcaggca aaataaccgc tgcgatttca ggggttttct tgttgttgat
    17461 tgtcgtggcg atcattttga ttgcaacttt tgactggaat cgactcaaac cgaccatcaa
    17521 ccagaaagtc tctgcggagt tgaatcgtcc gttcgctatc cgtggcgatc tgggcgtggt
    17581 gtgggagcgg caaaaacaag aaactggctg gcgcagctgg gtgccgtggc ctcatgtaca
    17641 tgcagaagac atcattcttg gtaatccacc ggatattccc gaagtcacga tggtgcattt
    17701 gccgcgagta gaggcaacgc tggcaccgct ggcgctgctg accaaaacgg tctggctgcc
    17761 gtggatcaag ctcgaaaagc ccgacgcgcg cctgattcgc ctctctgaaa agaacaataa
    17821 ctggacgttt aatcttgcca acgatgacaa caaagacgcg aatgcaaagc cgtcggcatg
    17881 gtcgtttcgg ctggataata ttcttttcga tcaagggcgg atcgccattg atgacaaagt
    17941 aagcaaagcg gatctggaaa tttttgttga tccgttaggc aagccgctgc cattcagcga
    18001 agttactgga tcgaaaggta aagcggataa agaaaaggtg ggcgattaca tttttggcct
    18061 gaaggcgcag ggacgttata acggtgaacc gctcacgggt acgggaaaaa taggcggtat
    18121 gctggcgctg cgtggcgaag ggacgccgtt tccggtacag gctgatttcc gttcaggtaa
    18181 tacccgtgtt gcttttgatg gcgtcgtgaa tgacccaatg aagatgggcg gtgtcgattt
    18241 acggcttaaa ttttctggcg attcactggg tgatctctat gaactgacgg gcgttctgct
    18301 gcccgatacc ccgccgtttg aaacggatgg tcggctggta gcgaaaatcg acactgaaaa
    18361 atcgtcggtc tttgattatc gcggttttaa tgggcgaatt ggcgatagcg atatccacgg
    18421 ttctctggtc tacaccaccg gaaagccacg accaaaactg gaaggtgatg tcgagtcgcg
    18481 gcaattgcgg ctggcggacc tgggaccgtt gattggcgtt gattccggga aaggggcaga
    18541 aaagtcgaaa cggtctgaac agaagaaggg cgaaaaaagc gttcagcctg cgggcaaagt
    18601 gctgccttat gaccgcttcg aaaccgataa atgggacgtt atggatgccg atgttcgctt
    18661 caaagggcgg cgcattgagc atggcagtag cctgccgatt agcgatcttt ctactcatat
    18721 catcctcaaa aatgctgacc tgcgcctgca accgctgaaa tttggcatgg cgggcggcag
    18781 cattgcggcg aatattcatc tggaaggcga taaaaagccg atgcaggggc gggcagatat
    18841 tcaggctcgt cgactgaaac tgaaagaact gatgcccgat gtggaactga tgcagaagac
    18901 gctgggggaa atgaacggtg acgcggaact acgcggtagc ggtaactcgg tggcggcgct
    18961 tttaggcaac agtaacggca acctgaaact gttgatgaat gacgggctgg tgagccgcaa
    19021 cctgatggag attgttgggc tgaatgtcgg caactacatt gtcggtgcga tatttggtga
    19081 cgatgaggtg cgggtgaact gcgcggcggc gaatctgcat attgccaacg gcgtggcgcg
    19141 cccgcagatt tttgctttcg atactgagaa cgcgttgatt aatgttaccg gcacggcaag
    19201 ttttgcttcg gaacagctgg atttgactat tgatccggag agtaaaggga ttcggattat
    19261 cacactgcgt tcgccgctgt atgtgcgtgg gacgtttaaa aatccgcagg ctggggtgaa
    19321 agccggaccg ctgattgccc gtggtgctgt tgctgcggca ctggcaacgc tggtaacacc
    19381 ggcggcggcg ttactggcac tgatctcacc ttccgaaggg gaggctaatc agtgtcggac
    19441 gatattgtcg cagatgaaga agtgatgata attgccggat gtctgacatc cggcaattaa
    19501 acagatttag atttttggca aagtgtgctt tgttcatgcc ggatgcggcg tgaacgcctt
    19561 atccggccta caaatccttg tgaattcaat atattgtatg gaaattcagt cctgataagc
    19621 gtagcgcatc aggcttttca cacttattac aacgactgat gtcgcgtctc atgggtcagc
    19681 agcagggcga ttaacgtcaa gccagccatc gccgccagat ataaccccac cgcacctaac
    19741 ccgtagttag cctgcaacca agctgcgata tacggcgcaa cggaagcccc gagaatcgac
    19801 gatacgttgt aagagaacga tgctccggtg taacgcactt ctgtcggaaa cagctctggt
    19861 aacagcgcac ccatcggccc gaaggtcagc cccatcagac ttaaccccag cagcaggaag
    19921 gcaaaaacca gaatcgggtt gccagaaccg agcagcgggt taaaggcgaa cagcgcgaac
    19981 aggatgatca gcgtggtgat gatcaccatg cttttacggc gaccaaaggc atcagccagt
    20041 aatccggcga ctggcaccat cacgccaaaa ccaataactg ccatcatcag catccacaac
    20101 acttcgttac gcggcaggcc aagcccaact ggcgcggcgg cggtactgaa ggtcatagag
    20161 tagaccgtca tgatgtaaaa cagcgtatag gttgccagca taatgaacgt acccagtacg
    20221 gttacgcgaa catgtttggt cagcagcgta cccagcggga tcttcacctg ttttttcgct
    20281 ttagcgactt tctcaaacac cggcgactca tgcagcgaca cgcgaacata caggccgata
    20341 atgaccagca ccgccgagaa gataaatggc acacgccagc cccagctcat aaactgctca
    20401 tcggtcagta gccaggaaag cagcaagaaa gtgccgttgg caaagaagaa gccaatcggt
    20461 gcgcctagct gtgggaagga gccatacagt gcacgtttgc gcggtggggc gttttcagtc
    20521 gccagcagcg ccgcgccgcc ccattcaccg cctaagccca gaccctgacc aaatcgagcc
    20581 agcgccagca gcagtggggc gaaaatacca atcgtggcat agcccggcag cagaccaatc
    20641 accacggtcg aaatccccat cgttagcaac gaggcgacca gcgtcgcttt acgcccaacg
    20701 cgatcgccaa aatgaccaaa aacggcagag ccaatggggc gcgcgacgaa ggcgatggcg
    20761 aaggtggcga gcgactgtag cgttgctgcc gttggatcgc cctgcgggaa gaagatatgc
    20821 ggaaacacaa taacggccgc agtggcgtaa atgtaaaagt cgaagaactc aatggccgtg
    20881 ccaatgagag aggcgacaag gactttatta cgcgagttga tcggcgtgta ttcttgttcg
    20941 tggtcgagtg ttgtggctgt tgcttgcata atcttttctt attttttggc taacgaatag
    21001 ccatattacg cacagcaaaa gtgatatttc aatgtgtaac aaatcgcgga aatggtcaaa
    21061 aaccaggcaa aaaggggata atgtttaggc cagtgatttt tcttctatga aatgcttcac
    21121 ataattttat aaatagtgga taaaactggt ttcaggttaa ataaaagtta ccaactggat
    21181 ttatatgctg gggttatgag ttggagtgaa attgtttgct cccaaataga tggctgaaga
    21241 ggctctgggg aagagtttca agaggttatg tgcgcattat agccggatgc gatgctggcg
    21301 catcttatcc ggcctacggg gagccaggcc gaataaaatc gtatgccgga taaggcgttt
    21361 acgccgcatc cggcctattt taaggctgcg ttttccctgg cattcgcggg tcgtctttat
    21421 attcggcggt ggcaatccac gccgcgcaaa acagcgtcaa acgggcgaag aagtagaaaa
    21481 atgccatcag ccccagcacg gaaccaaatg ctgcgccaga gggggatttc atcagcgatg
    21541 gcagggtgta ggtcatcacg attttaatca cttcaaaacc aatagcagcc agaaatgttc
    21601 cgcgaatcag ggcttttttg cgcggacggt ggcgcggcaa ccgccagaag atccagaaga
    21661 aaagcagata gttggcgaag atggaaatcg ccaggccaat caatcgccac gtcggtttca
    21721 gccactcaat gctgttcagg tgcagggcgc taataatcat ttgctgcgcc gaaccggcaa
    21781 ccgaggtgat cgaaagcgtc acaatcagcg caatcaacaa accaatcagt gaaataaaat
    21841 cacgcagata ttttacccag aacttctcct gatcttgcgg cgagcgttcc cagacatcgc
    21901 gcgactgggc acgaatcgct tcacgcaggt tacccatcca gttgatgccg gaataaagtg
    21961 ccaccgccag gccgacaagc cctacagtcg tacgctgctg aacggcggtg ttgatggtgt
    22021 ttttcaacgt ggcggctagt gtcggatcgc tgatgttttg cagaattttg tcgaagatat
    22081 cctgtagcaa catcggatgg gaggccagca caaagccccc tgcggcaaac gacaccatca
    22141 aaatcgggat catcgacaaa aatgagaaat aggtgatagc cgcaccaaac tggttaccca
    22201 gccgatcgtt aaagcgttct gtcgcgcgaa taaggtgcgc aataaccggt tggcgttgga
    22261 ctttttcggc agtggtggtg acggtttcca gcgcctggct aactttgccg cctttctcgc
    22321 tattatccaa ctgcttaatc ggctcgtgct ccagatcctg ggtgggacgt ttgatctcgt
    22381 tttcctgcgt catccgctct tttgtccttc tgtcgtggtc attcttaaaa agtatagtcg
    22441 gtcagtccag gtgctctttc attacgccag ccagccactg cataaacagg tttacacgcc
    22501 gggaaagctc ccgacgctgc gggtaaacca gcgaaaggga gagcggctcg gcacggtagc
    22561 ctggcaacac ttcaataagc cgcccggcac gcagggcttc gcgcacggca atgcgcggga
    22621 tctgaataat ccccagaccg gcaagaccgg cggcgagata gttttcgcta ctgtttaccg
    22681 tcaacatgcc gccagactta aaccactgga cgccattaac gctggcaacc tcaaaaccta
    22741 acggatgtac acccaggtgc ggtgtataac gcactattgc gtgtgaagtc agatcatcgg
    22801 ggctttgcgg ataaccaaag cgcgtcagat agtgcggact ggcacagttg accatggtta
    22861 gtttgccgag gggacgggcg ataacgccat cttccggtaa cgcgccagtg cgtatcacgc
    22921 aatcaaaacc atcatgaaga atatctaccg gacggttatg gctactcagt tccagctcaa
    22981 ttcccggatg ctgatagaga aattccgaca gacgcggcag taacaggttt ttcgcgattc
    23041 ctgacgggat gtcgatgcgt aatttaccgc tgatactggt ggcatcctgt tgaaacagac
    23101 cgtccagttc gctgagatta ctcaacacat cttttgcccg ttgataatag gtcatgcctt
    23161 ctggcgtgag tttgacccgt cgcgtagtgc gctggagaag ctgggtgcca agttgatgtt
    23221 ccagtgcctg tatctggcgc gaaacacttc cctttggcaa agcaaagaaa tccgccgcgc
    23281 gggaaaaact ttccagctcc gcgactttga tgaacaactg cattgcgtga attttatcca
    23341 tttctttgac cgattgttgt tcacagcgaa acagtaacgc gcatatcgca ttgtttattg
    23401 ttttttctaa catctaatgc cgggtttcac aatgttgtca tgacgaatgt ggcgatttct
    23461 cagcaggaaa agggcggaat agctttatga gaaaggtgaa tccccttaaa cagagcgtat
    23521 ttttcagtga ttccctttaa agtgaataga ggacgtattg gctatttgtg cttatttttg
    23581 tctcattgcc atttgttatt ttggcttgta gtcgcggaat ttcattttta attgcatgcg
    23641 tactaaccac caatataagg aaatcctcaa aattgtttta aatagtgccc tcgacatgta
    23701 atttcaggcg tttagctgta actatttata tcggtaacta ttttctacgc gtcatctctg
    23761 tagaatggcc accgccaaat ggcgaattct tattttttct cgcgggtaat acccttccac
    23821 tacttaagtc tttttaacgt attcaggtgg agattcagtt atgcaaatag tcatgtttga
    23881 caggcagtca atatttattc atggaatgaa aatcagttta cagcagcgta ttccaggagt
    23941 gagtattcag ggggtcagtc aggcagacga gttatggcaa aagctggaaa gttaccctga
    24001 agccttagtt atgctcgatg gcgatcagga tggtgagttt tgctactggt tgctgcaaaa
    24061 aaccgtggtg cagtttcctg aggttaaggt gttaatcacg gcgacggatt gcaataaacg
    24121 gtggttacag gaagttatcc attttaatgt gctggccatt gtgcctcgcg attcaaccgt
    24181 cgagacattt gcgttggcgg taaatagtgc ggcgatgggg atgatgtttc tgcctggaga
    24241 ctggcggacg acgccggaaa aggacattaa agatctcaaa tcgttaagcg cccgccagcg
    24301 ggagatttta accatgttag cagcgggcga atcaaataag gagatcggca gagcgctgaa
    24361 tatcagtacc ggaacggtaa aagcgcatct ggaatccctg tatcgccgtc tggaagtgaa
    24421 aaatcgcacc caggcggcaa tgatgttaaa t
//
LOCUS       DAJTYF010000051        24193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTYF010000051 DAJTYF010000000
VERSION     DAJTYF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.051"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(25..597)
                     /locus_tag="OO590_002176"
     CDS             complement(25..597)
                     /locus_tag="OO590_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6637536.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="OO590_002177"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="OO590_002177"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6637537.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OO590_002178"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OO590_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6637538.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OO590_002179"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OO590_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6637539.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="OO590_002180"
     CDS             complement(3559..5340)
                     /locus_tag="OO590_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6637540.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="OO590_002181"
     CDS             complement(5333..7105)
                     /locus_tag="OO590_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6637541.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OO590_002182"
                     /pseudo
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OO590_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OO590_002183"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OO590_002183"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6637542.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10364
                     /locus_tag="OO590_002184"
                     /pseudo
     CDS             8665..10364
                     /locus_tag="OO590_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10429..11124
                     /gene="queC"
                     /locus_tag="OO590_002185"
     CDS             10429..11124
                     /gene="queC"
                     /locus_tag="OO590_002185"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6637543.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11176..11574)
                     /gene="fadM"
                     /locus_tag="OO590_002186"
     CDS             complement(11176..11574)
                     /gene="fadM"
                     /locus_tag="OO590_002186"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6637544.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11668..12039)
                     /locus_tag="OO590_002187"
     CDS             complement(11668..12039)
                     /locus_tag="OO590_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6637545.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNLLVERNLAVLTL"
     gene            complement(12190..14061)
                     /gene="ppiD"
                     /locus_tag="OO590_002188"
     CDS             complement(12190..14061)
                     /gene="ppiD"
                     /locus_tag="OO590_002188"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6637546.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14253..14525)
                     /gene="hupB"
                     /locus_tag="OO590_002189"
     CDS             complement(14253..14525)
                     /gene="hupB"
                     /locus_tag="OO590_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6637547.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14734..17088)
                     /gene="lon"
                     /locus_tag="OO590_002190"
     CDS             complement(14734..17088)
                     /gene="lon"
                     /locus_tag="OO590_002190"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6637548.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17276..18550)
                     /gene="clpX"
                     /locus_tag="OO590_002191"
     CDS             complement(17276..18550)
                     /gene="clpX"
                     /locus_tag="OO590_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6637549.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18676..19299)
                     /gene="clpP"
                     /locus_tag="OO590_002192"
     CDS             complement(18676..19299)
                     /gene="clpP"
                     /locus_tag="OO590_002192"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6637550.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19546..20844)
                     /gene="tig"
                     /locus_tag="OO590_002193"
     CDS             complement(19546..20844)
                     /gene="tig"
                     /locus_tag="OO590_002193"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6637551.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21188..21505)
                     /gene="bolA"
                     /locus_tag="OO590_002194"
     CDS             complement(21188..21505)
                     /gene="bolA"
                     /locus_tag="OO590_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6637552.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21810..22388
                     /locus_tag="OO590_002195"
     CDS             21810..22388
                     /locus_tag="OO590_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6637553.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22432..23907
                     /gene="ampG"
                     /locus_tag="OO590_002196"
     CDS             22432..23907
                     /gene="ampG"
                     /locus_tag="OO590_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6637554.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5668 a         6663 c         5838 g         6024 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacgg taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta ttgcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 agatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccgctga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcccacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataatt gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tcagccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacgt tggcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg caagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgttgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggagatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actacgccta ctgccgcccg attaaacagc agcgactgag cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccag ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgagttc gctaccatcg ttcaccaccg gcgcttccgc cagcatcgcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggat cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctagacg cgcatcagat aatcgtattc accagccatg cgccagaacc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtcag
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc tgccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgg aaagccgcaa accgtcaccg tcagcgaact ggccgaacgc
     8761 tgtttttgca gcgaacgcca tgttcgtacg ctgttgcgtc aggcacagga ggcgggatgg
     8821 ctggagtggc aggcgcagtc aggacgcgga aagcgcggac aattacgctt tctggtcacg
     8881 ccggaatcgc tacgcaatgc gatgatggaa caggcactgg aaaccggaaa gcagcaagat
     8941 gtgctggagc tggcgcaact ggccccaggt gagctgcgca ctctgttaca gccgtttatg
     9001 ggcggacaat ggcaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac
     9061 cgctacaacc aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt
     9121 ccggcctgac ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact
     9181 gggaaacctc tactgacggg ttacgctggg acttttatct tcgttcaacc ctacactggc
     9241 ataacggcga tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac
     9301 tgccagcact ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt
     9361 gtctgacctt ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt
     9421 gcagccatct ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac
     9481 aattcacagc ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc
     9541 tgcttaaagc ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca
     9601 gttgtcggca tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc
     9661 aggtcagtag tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac
     9721 tctccctctg gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa
     9781 cgttagaagt cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta
     9841 ttccgcactg gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc
     9901 acctaccgat agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag
     9961 gctgtgaact cacaattatt tttcataacg caaaaaactg ggacgacacg accctactgg
    10021 cacacgcaga cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg
    10081 agcaatggct acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac
    10141 atctgcaatc gacgctggac gcggtgcaag taatgcctga tgaggaaaac cgatttaatg
    10201 ccctgaaagc ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact
    10261 atcactatcg cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct
    10321 ggtttgaatt taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt
    10381 aatcgcaggc gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct
    10441 gtcgttgtgt tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa
    10501 tatgatgaag tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac
    10561 gtggcacgcg aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg
    10621 ctgctcaacg agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat
    10681 gaacctgaag ccgatggcat cccgaatacg tttgtcccag ggcgtaatat tttgttcctg
    10741 acgctggcgg caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt
    10801 gaaacggatt tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat
    10861 gccgtcagtt tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat
    10921 aaagcggaaa cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa
    10981 acgttgacct gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat
    11041 ttacgcgcca acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg
    11101 aagcagaaaa ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc
    11161 ggcaccacaa aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag
    11221 agctaatgct ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac
    11281 cacctgccct tccggctcca gtgtaatgac ctggcttaag atgccgcttt taccgtttaa
    11341 ttgctgcaac tgactggtaa tagttaacag gtcgcttaat accgctgggc gacgatagtt
    11401 aatattgata ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc
    11461 gctattttcc aacccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt
    11521 gacgtgctgg taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca
    11581 ataaccttac gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg
    11641 gcaaaaaatt gccactatgc aaattaatta cagggttaat accgccagat tacgttccac
    11701 cagcaaattg cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta
    11761 ctcttcgcga taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg
    11821 ggctagctct tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc
    11881 tttcgccggt actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgc
    11941 cgtcggtttc gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag
    12001 ggacagggta atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt
    12061 aacagtggaa tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg
    12121 gaaaaggccg cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg
    12181 gcttcaggat tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag
    12241 gttactcatc agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat
    12301 cgcttttttc tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac
    12361 cacattacct tgcatatcag tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg
    12421 cagtgcaaac gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc
    12481 gccaaatttc agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac
    12541 cagcagtttc tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc
    12601 cttaacttgt tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgac
    12661 gcgcagcacg aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccgacgc
    12721 gccgttttca cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa
    12781 ctcttccggc aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc
    12841 ttgctctgca ccggccagag actcggtttc gttgcttgcc gcatcgctca ctttctgctg
    12901 cagcgcatag tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg
    12961 tacttcgtct aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa
    13021 accgaccgaa gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt
    13081 cagttcgtcg gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga
    13141 gataatatcg gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc
    13201 atcaagtacc gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg
    13261 ctgcggctgg gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct
    13321 aaccggttgc tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga attgttccgg
    13381 cgtcatgaaa ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg
    13441 ctgcttcgcc gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc
    13501 gaccagtgcc gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacgcc
    13561 gttaatcagc tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt
    13621 catccccatc tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg
    13681 gaaggctggg gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag
    13741 ctcacgagcg tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg
    13801 cagggttttc atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg
    13861 ctgcatgcga ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg
    13921 gtcattcact tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt
    13981 caatatgaac gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc
    14041 cgtgcgtaag ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca
    14101 cgcaacgtat cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc
    14161 cacaagacac agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt
    14221 gaacttcgtc acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta
    14281 cctgcacgga agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg
    14341 ttgcggccag tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct
    14401 acatcatccc cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg
    14461 ccagccgcag ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta
    14521 ttcactcttc tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg
    14581 cgcagcagtt atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg
    14641 atccgctatc taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc
    14701 cctgttttta ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc
    14761 agacggttca ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat
    14821 gtccagatcg gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg
    14881 aattagcact gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc
    14941 gatcggcagt acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac
    15001 cgggttaccg gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc
    15061 atctttcggc gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc
    15121 agggttgatc cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga
    15181 ctcctgcatc acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca
    15241 tgcggtttca atggtcagca agtcaccgcc cacttccgtc cacgccagac cggttacctg
    15301 accgacacgg ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc
    15361 atgcaggtta tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt
    15421 aaccgctttg cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg
    15481 ggtgtagtaa cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt
    15541 cagtgcatta cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc
    15601 atcttcggta taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat
    15661 gttcatggag ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag
    15721 gtagtggtcg ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc
    15781 cggatcgcct cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcgggtt
    15841 tttcacgccc actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt
    15901 acggcggtga ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata
    15961 tttacgcccg gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg
    16021 ccctaccagg cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta
    16081 ctcaaggatg cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg
    16141 cgcctgacgc aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca
    16201 gtcgatataa ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt
    16261 ctgcaactct gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt
    16321 gcgcttcagg gcttcgtttt cgtccggcgc gtcgtccatt tcaccgagtt ctttctgaat
    16381 agctttcatt tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac
    16441 gcggttgcga atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat
    16501 cagatattcc agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag
    16561 tttcagcggc atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt
    16621 cagcgacgtc agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg
    16681 gctgattgca gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag
    16741 atactccgcc ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg
    16801 ctgtaacccc tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat
    16861 agaggccacg gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc
    16921 tttctgcgcg accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat
    16981 agatttttcc cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg
    17041 cagcggcaat acggggattt caatgcgttc agaacgctca ggattcatag agctctctct
    17101 tagtttaatt tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca
    17161 tgtacgtcag tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt
    17221 aaaggggaga taaaatcccc cctttttgtt taactaattg tatgggaatg gttaattatt
    17281 caccagatgc ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat
    17341 caattaccga ctcgtcaata accacttttt cgacgtcttc catggacggc agatcgtaca
    17401 tggtatcgag cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg
    17461 ccatcgcttt cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt
    17521 ccagattaaa cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct
    17581 gaatcagagc ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga
    17641 actcagggat aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc
    17701 cttcgcttgc tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg
    17761 tttctacacg gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca
    17821 ggatcttaga ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg
    17881 gtggaacagc agctaccgta ccttcgatca gtttcaacag tgcctgctgt actccttcac
    17941 cggaaacgtc tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat
    18001 cgatgtagac aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct
    18061 tctgaatgat gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt
    18121 cggccatggt gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac
    18181 cggaaccggt cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg
    18241 tatcgccgtt gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg
    18301 cctgttcctg gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta
    18361 gcgcactgcg ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac
    18421 ataaatcaac acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt
    18481 catgctggct tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt
    18541 tatctgtcat gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg
    18601 ccattctcag gcgcaagccg cttacagcgt tgtgccgccc tagataagta tagcggcaca
    18661 gttgcgcctc tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac
    18721 cgcttcaggg gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga
    18781 ttgacccgta tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg
    18841 agcatgaatt tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat
    18901 cacgcgcgaa ttcggcaggc aaaaacgttt accttttgcc cctgcggtca acaagaaagc
    18961 gcccatcgag gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat
    19021 ggtgtcatag atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata
    19081 gatatctttt tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc
    19141 catgtggtct tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata
    19201 gatatcaaaa gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat
    19261 atggggtgca aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt
    19321 gaaaaaaacc tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt
    19381 tatgggtacg gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg
    19441 aaagaggctt ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc caccacctgg
    19501 cggtgacggg cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt
    19561 catcagctcg ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc
    19621 aacagcctgt tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct
    19681 gtagaactcg ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc
    19741 tttcacgcgc tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag
    19801 gccaacaact acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc
    19861 ttgtttttcg ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct
    19921 gtcgatcagc gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg
    19981 agacttaacg cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc
    20041 agcgcgcaga ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt
    20101 cagttccggc agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt
    20161 acctttcagg ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc
    20221 gccagctttg tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat
    20281 cgccagtacg aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt
    20341 gaagtcgatg gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc
    20401 ctgctgttta cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat
    20461 cggtttttca acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa
    20521 ctctacagag taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc
    20581 gccagccgga ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc
    20641 acccagaacg tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac
    20701 tttgcctttg cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt
    20761 aacagcggtc tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg
    20821 agtggtttca actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact
    20881 cttaccccgc aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca
    20941 cattccctgt taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc
    21001 atcactttta tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc
    21061 gggcaatttt cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc
    21121 cgcaggccgt ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa
    21181 tgcgttttta cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct
    21241 gcaacccttc ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag
    21301 agagttcctc cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg
    21361 taaaacgatc gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt
    21421 gacgatagct ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt
    21481 cttctatccg ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc
    21541 atccctttaa atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag
    21601 gtttcgtcct ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca
    21661 ggcctcgcat cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac
    21721 atgccttacc cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa
    21781 cacgcttaag agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg
    21841 ctctgtttat gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga
    21901 ttacactgcc acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg
    21961 atcagcgtac agatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga
    22021 ccgcctcccg cgatctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc
    22081 gtggttacat ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt
    22141 atgctgacgt gtcccagggc aacgtgcgct acaacatcgc taccaaagcg gatatcgcca
    22201 tcatcgctac cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg
    22261 ttgaaggtgc gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga
    22321 ccgataccat cgctgatatg tctcaggaca ccagcattca cgaattcatc aagcagaacg
    22381 cgcgttaatt tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt
    22441 caatatttac gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct
    22501 tccgggctac cgctcgccct gacatccggc accttacagg cctggatgac ggtcgaaaat
    22561 atcgatctca aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc
    22621 ctctggtcac cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg
    22681 ctgctcgcca cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca
    22741 ggcacccaac tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc
    22801 caggatattg tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg
    22861 ggcgcggcaa tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcctagcc
    22921 ctgtggctgg cagataaatg gttgggctgg cagggcatgt actggttgat ggcggcgctg
    22981 ttgatcccct gtattatcgc gacgttgctt gcaccagaac caaccgacac cattcctgtg
    23041 cccaaaacgc tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat
    23101 gcctggctta ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc
    23161 ctgacaacca cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta
    23221 aacaaaacgc ttggcttact ggcgaccatt gttggcgcat tgtacggtgg gattttgatg
    23281 cagcgcctgt cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac
    23341 gctggttact ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc
    23401 tttttcgaaa acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg
    23461 ctgtgtaata agtcattttc cgctactcaa tttgccctgc tctcagcgct ttccgctgta
    23521 gggcgagttt atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca
    23581 ttctatctat tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc
    23641 cagacgcttg aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca
    23701 ggttatgcct ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta
    23761 cttctgttga cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg
    23821 gaagtcgggg ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg
    23881 gcgctacgaa aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc
    23941 gccgtaatta ttacggctaa ataaacatca gcatcgctta ttaatatttg ttcttttgtg
    24001 cggcttagcg tttggaaatt attggcgcca tttataaatt ctatttttct tacacgattc
    24061 agctaatgag tctttatttt ctcaccaccc agttgtcact ctaatgataa ttatttgtta
    24121 aataattgtt ttatttcgca ttggttatac caattgcccg ccaagactcc ttctgcactc
    24181 cccttttgtt ata
//
LOCUS       DAJTYF010000052        24115 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTYF010000052 DAJTYF010000000
VERSION     DAJTYF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.052"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..1271
                     /gene="glpK"
                     /locus_tag="OO590_002197"
     CDS             <1..1271
                     /gene="glpK"
                     /locus_tag="OO590_002197"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6637555.1"
                     /translation="TNQRETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLEDY
                     ISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVT
                     DYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPI
                     SGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGE
                     VNYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLSAPY
                     WDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAV
                     ANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFR
                     PGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            1501..2511
                     /gene="glpX"
                     /locus_tag="OO590_002198"
     CDS             1501..2511
                     /gene="glpX"
                     /locus_tag="OO590_002198"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6637556.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLNRKDPEMQVH
                     IL"
     gene            2608..3354
                     /gene="fpr"
                     /locus_tag="OO590_002199"
     CDS             2608..3354
                     /gene="fpr"
                     /locus_tag="OO590_002199"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6637557.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(3359..3787)
                     /gene="uspD"
                     /locus_tag="OO590_002200"
     CDS             complement(3359..3787)
                     /gene="uspD"
                     /locus_tag="OO590_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6637558.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLENKSDNKLYKLTKNIQCPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(3814..4113)
                     /locus_tag="OO590_002201"
     CDS             complement(3814..4113)
                     /locus_tag="OO590_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6637559.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(4325..4765)
                     /locus_tag="OO590_002202"
                     /pseudo
     CDS             complement(4325..4765)
                     /locus_tag="OO590_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            4866..5465
                     /locus_tag="OO590_002203"
     CDS             4866..5465
                     /locus_tag="OO590_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6637560.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            5573..6340
                     /gene="tpiA"
                     /locus_tag="OO590_002204"
     CDS             5573..6340
                     /gene="tpiA"
                     /locus_tag="OO590_002204"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6637561.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(6395..7150)
                     /gene="cdh"
                     /locus_tag="OO590_002205"
     CDS             complement(6395..7150)
                     /gene="cdh"
                     /locus_tag="OO590_002205"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6637562.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKSVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(7257..8246)
                     /gene="sbp"
                     /locus_tag="OO590_002206"
     CDS             complement(7257..8246)
                     /gene="sbp"
                     /locus_tag="OO590_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6637563.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(8566..9528)
                     /gene="pfkA"
                     /locus_tag="OO590_002207"
     CDS             complement(8566..9528)
                     /gene="pfkA"
                     /locus_tag="OO590_002207"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6637564.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(9709..10611)
                     /gene="fieF"
                     /locus_tag="OO590_002208"
     CDS             complement(9709..10611)
                     /gene="fieF"
                     /locus_tag="OO590_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6637565.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(10759..11259)
                     /gene="cpxP"
                     /locus_tag="OO590_002209"
     CDS             complement(10759..11259)
                     /gene="cpxP"
                     /locus_tag="OO590_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6637566.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            11409..12107
                     /gene="cpxR"
                     /locus_tag="OO590_002210"
     CDS             11409..12107
                     /gene="cpxR"
                     /locus_tag="OO590_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6637567.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            12104..13477
                     /gene="cpxA"
                     /locus_tag="OO590_002211"
     CDS             12104..13477
                     /gene="cpxA"
                     /locus_tag="OO590_002211"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6637568.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(13583..14257)
                     /gene="yiiM"
                     /locus_tag="OO590_002212"
     CDS             complement(13583..14257)
                     /gene="yiiM"
                     /locus_tag="OO590_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6637569.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(14406..15389)
                     /gene="kdgT"
                     /locus_tag="OO590_002213"
     CDS             complement(14406..15389)
                     /gene="kdgT"
                     /locus_tag="OO590_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6637570.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(15649..16269)
                     /gene="sodA"
                     /locus_tag="OO590_002214"
     CDS             complement(15649..16269)
                     /gene="sodA"
                     /locus_tag="OO590_002214"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6637571.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            16554..17588
                     /gene="rhaT"
                     /locus_tag="OO590_002215"
                     /pseudo
     CDS             16554..17588
                     /gene="rhaT"
                     /locus_tag="OO590_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(17585..18451)
                     /gene="rhaR"
                     /locus_tag="OO590_002216"
     CDS             complement(17585..18451)
                     /gene="rhaR"
                     /locus_tag="OO590_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6637572.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(18507..19343)
                     /gene="rhaS"
                     /locus_tag="OO590_002217"
     CDS             complement(18507..19343)
                     /gene="rhaS"
                     /locus_tag="OO590_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6637573.1"
                     /translation="MTVLRSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            19631..21100
                     /gene="rhaB"
                     /locus_tag="OO590_002218"
     CDS             19631..21100
                     /gene="rhaB"
                     /locus_tag="OO590_002218"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6637574.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            21097..22356
                     /gene="rhaA"
                     /locus_tag="OO590_002219"
     CDS             21097..22356
                     /gene="rhaA"
                     /locus_tag="OO590_002219"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6637575.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            22598..23422
                     /gene="rhaD"
                     /locus_tag="OO590_002220"
     CDS             22598..23422
                     /gene="rhaD"
                     /locus_tag="OO590_002220"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR6637576.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            23432..23746
                     /gene="rhaM"
                     /locus_tag="OO590_002221"
     CDS             23432..23746
                     /gene="rhaM"
                     /locus_tag="OO590_002221"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6637577.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            24047..>24115
                     /locus_tag="OO590_002222"
     CDS             24047..>24115
                     /locus_tag="OO590_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6637578.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLK"
BASE COUNT         5985 a         6296 c         6133 g         5701 t
ORIGIN      
        1 ttacgaacca gcgtgaaacc actattgtct gggaaaaaga aaccggcaag cctatctata
       61 acgccattgt ctggcagtgc cgtcgtaccg cagaaatctg cgagcattta aaacgtgacg
      121 gtttagaaga ttatatcagc agcaataccg gtctggtgat tgacccgtac ttttctggca
      181 ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg cgagcgtgca cgtcgtggtg
      241 aattgctgtt tggtacggtt gatacgtggc ttatctggaa aatgactcag ggccgtgtcc
      301 atgtgaccga ttacaccaac gcctctcgta ccatgttgtt caacatccat accctggact
      361 gggacgacaa aatgctggaa gtgctggata ttccgcgcga gatgctgcca gaagtgcgtc
      421 gttcttccga agtatacggt cagactaaca ttggcggcaa aggcggcacg cgtattccaa
      481 tctccgggat cgccggtgac cagcaggccg cgctgtttgg tcagttgtgc gtgaaagaag
      541 ggatggcgaa gaacacctat ggcactggct gctttatgct gatgaacact ggcgagaaag
      601 cggtgaaatc agaaaacggc ctgctgacca ccatcgcctg cggcccgact ggcgaagtga
      661 actatgcgtt ggaaggtgcg gtgtttatgg caggcacatc cattcagtgg ctgcgcgatg
      721 agatgaagtt gattaacgac gcctacgatt ccgaatattt cgccaccaaa gtgcaaaaca
      781 ccaatggtgt gtatgtggtt ccggcattta ccgggctgag tgcgccgtac tgggacccgt
      841 atgcgcgcgg ggcgattttc ggtctgactc gtggggtgaa cgctaaccac attatacgcg
      901 cgacgctgga gtctattgct tatcagacgc gtgacgtgct ggaagcgatg caggccgact
      961 ctggtatccg tctgcacgcc ctgcgcgtgg atggtggcgc agtagcaaac aatttcctga
     1021 tgcagttcca gtccgatatt ctcggcaccc gcgttgagcg cccggaagtg cgcgaagtca
     1081 ccgcattggg tgcggcctat ctcgcaggcc tggcggttgg cttctggcag aacctcgacg
     1141 agctgcaaga gaaagcggtg attgagcgcg agttccgtcc aggcatcgaa accactgagc
     1201 gtaattaccg ttacgcaggc tggaaaaaag cggtcaaacg cgcgatggcg tgggaagaac
     1261 acgacgagta atgtcaggca cctgaatcga ttgcctgatg ctgatcgcct gatgcgacgc
     1321 ttacgcgtct tatcaggcct acgagccaat gccattttgt gtaggcggga taaggcgttc
     1381 acgccgcatc cggcatcacg cagactctga aaccgtgcgg gggcaacccc gcacacatca
     1441 ataatcccac ccttcccctg tgctacactt cgcgccattc cttactgctt agagtttgct
     1501 atgagacgag aacttgccat cgaattttcc cgcgtcaccg aatctgcggc gctggctggc
     1561 tacaaatggt taggacgcgg cgataaaaac accgcggacg gcgcggcggt aaacgccatg
     1621 cgtattatgc tcaaccaggt caacattgac ggcaccatcg tcattggtga aggtgaaatc
     1681 gacgaagcac cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta
     1741 gatattgctg ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg
     1801 ctggcggtgc tggcagtagg cgataaaggc tgcttcctca atgcgccgga tatgtatatg
     1861 gagaagctga ttgtcgggcc gggagccaaa ggcaccattg atctgaacct gccgctggcg
     1921 gataacctgc gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg
     1981 attctggcta aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc
     2041 gtatttgcta ttccggacgg cgacgttgcg gcctcaattc tcacctgtat gccagacagc
     2101 gaagttgacg tgctgtacgg tattggtggc gcgccggaag gcgtggtttc tgcggcggtg
     2161 atccgcgcat tagatggcga catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc
     2221 gacaacgaag agaatcgtcg cattggcgag caggagctgg cacgctgcaa agcgatgggc
     2281 atcgaagccg gtaaagtatt gcgtctgggc gatatggcgc gcagcgataa cgtcatcttc
     2341 tctgccaccg gtattaccaa aggcgatctg ctggaaggta ttagccgcaa aggcaatatc
     2401 gcgactaccg aaacgctgct gatccgcggc aagtcacgca ccattcgccg cattcagtcc
     2461 atccactatc tgaatcgcaa agacccggaa atgcaagtgc acattctctg agtgatttga
     2521 tcgattgagc cttccagtcc ttcgggactg gaattttttt gttcggagaa cgaagataag
     2581 gcaagtcaat caaaacagga gaaaaaaatg gctgattggg taacaggcaa agtcactaaa
     2641 gtgcagaact ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt gcttccgttt
     2701 accgccgggc aatttaccaa gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc
     2761 tactcctatg ttaactcgcc cgataatccc gatctggagt tttacctggt caccgtcccc
     2821 gatggcaaat taagcccacg attggcggca ctgaaaccgg gcgatgaagt gcaggtggtt
     2881 agcgaagcag ctggcttctt tgttctggat gaagtaccgg actgcgaaac gctatggatg
     2941 ctggcaaccg gtacagcgat tggcccttat ttatcgattc tgcaactagg caaagattta
     3001 gatcgcttca aaaatctggt cctggttcac gccgcacgtt atgccgccga cttaagctat
     3061 ttgccactga tgcaagaact ggaaaaacgc tacgaaggca aactgcgcat tcagacggtg
     3121 gtcagtcggg aaacggcagc ggggtcgctc accggacgga taccggcatt aattgaaagt
     3181 ggggaactgg aaagcgcgat tggcctgccg atgaataaag aaaccagcca tgtgatgctg
     3241 tgcggcaatc cacagatggt gcgcgataca caacagttgc tgaaagagac ccggcagatg
     3301 acgaaacatt tacgtcgccg accgggccat atgacagcgg agcattactg gtaagcggtt
     3361 acttatcgat aaacggcacg atgagcaaat ccgcactcat cttattgatc atcccgcgat
     3421 atgccggcat caaacggttg ataaatgagt gatgatgacc acagacaagg aggtcgcact
     3481 gctctttttg cataatttcc agcagtgttt ccggcatttc tccgcgttca atacgcagtt
     3541 ttgtcttcgg acattgaata tttttcgtca gtttatacag cttgttatcc gacttattct
     3601 ccaacaattg aagaatatct tctgttgcag ggaagtagat acccggatac aactcgctta
     3661 agccatcatc aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg agctccaggg
     3721 ctttattcac cagtaaggca tcttcttcat tcccggaaat tgccacgcca atgtgtttat
     3781 aagccatgtt taactccttc taaagcaact ccatcaagct aacgaacgca gtgataactc
     3841 aaaaataatc atctcggcct ggcatgagaa agtgaaggcc gcatcaagct caacttgccc
     3901 ctccagatcc ttaatcttat aggccacgct ggccccttca tctgcagagg atgcagcggc
     3961 ggcaagctct ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg
     4021 cgaaaactta ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc
     4081 tttagtactg catttatcta cgacatcttt catagggcac ctctgtatat tcgcctctct
     4141 gtcgattacg acttaagaat aatgcgccgt taagggaaga acaaacaagt aatgatctta
     4201 tggattacca ttttttttga tattgcttaa ggtgaagagt tcagaaggat tttccgggta
     4261 ttgtcatttg catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag agatatatta
     4321 aaggttaatt acttgattta ttgtcggctt tatacttcac atcctgagta tctttaccat
     4381 atttattttc gccttgtgtg ccaacaaacg caccaagatc gataagcatc atcaccagaa
     4441 tcaacgtcgg gacaaaacgc cccaccgccc attgccagac acccggtaaa atcgctcagt
     4501 tacccgccag cagcatccac gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc
     4561 gatcatgcaa gcgcttaaca gttactgccg ctgttggcca gagcaagcac acaaggcaaa
     4621 acgccgcggt ctgaatatcg agtagattct tacccgccag tgagaaaagc accagcatgc
     4681 ctgcgaacca caggcctatc caaatccaga aatcacggcg tccaatacgc cctttaaatg
     4741 agaataacca ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt ccaggattct
     4801 acccttttga caagggcgac agatatcgtt ttaatcggag ccagtcataa caaaaggtac
     4861 tgtcaatgaa gccagggtgt acgctgtttt ttctcttatg ttctgcatta accgttacaa
     4921 caacggcgca tgcacaaaca ccagatacgg caacgaccgc gccttatctg ctggctggag
     4981 cccctacttt cgatctctcc atcagccagt ttcgagaaga ctttaacagc cagaatccca
     5041 gcctgccact gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc aatctcactc
     5101 gtgctgcaag taaaattaac gagaacttgt atgcttctac agcgctggag cgcggtacct
     5161 taaaaatcaa aagcattcaa atgacctggc tacccatcca ggggccagag caaaaagcag
     5221 cgaaagcgaa agctcaggaa tacatggcag cagtgatccg cacactcacc ccattaatga
     5281 ccaaaacaca aagccagaaa aaactgcagt cgctactaac ggcggggaaa aacaaacgtt
     5341 attacaccga gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc
     5401 tgaccttcgc tgttgaaccg attaagctgg cgctatctga atcgcttgaa ggtttgaata
     5461 aatgacaaaa agcaaagcct ttgtgccgat gaatctctat actgtttcac agacctgctg
     5521 ccctgcgggg cggccatctt cctttattcg cttataagcg tggagaatta aaatgcgaca
     5581 tcctttagtg atgggtaact ggaaactgaa cggcagccgc cacatggttc acgagctggt
     5641 ttctaacctg cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc
     5701 ggaaatgtac atcgatatgg cgaagcgcga agctgaaggc agccacatca tgctgggtgc
     5761 gcaaaacgtg gacctgaacc tgtccggcgc attcaccggt gaaacttctg ccgctatgct
     5821 gaaagacatc ggcgctcagt acatcatcat cggccactct gaacgtcgta cttaccacaa
     5881 agagtctgac gaactgattg cgaaaaaatt cgcggtgctg aaagagcagg gtctgactcc
     5941 ggttctgtgc atcggtgaaa ccgaagctga aaacgaagcg ggaaaaactg aagaagtttg
     6001 cgcacgtcag atcgacgcgg tactgaaaac tcagggtgct gcggcattcg aaggtgcggt
     6061 tatcgcttat gaacctgtat gggcaatcgg tactggcaaa tctgcaactc cggctcaggc
     6121 acaggctgtt cacaaattca tccgtgacca catcgctaaa gttgacgcta acatcgctga
     6181 acaagtgatc attcagtacg gcggctctgt aaacgcgtct aacgctgcag aactgtttgc
     6241 tcagccagac atcgacggcg cgctggttgg cggtgcttct ctgaaagctg acgctttcgc
     6301 agtgatcgtt aaagctgcag aagcggctaa acaggcttaa gtctgacagg tgccggattt
     6361 catatccggc acttactttc cttaactcct cgccttaacg caaaatctca cactcatgat
     6421 cctggatttc ctcggctgaa gcacggttca gcgtcagtag atttcgttgt gtcgccagca
     6481 acacaaacga attatcactc tgccgtacca tcgccagccc gtagcttccc atatgttccc
     6541 gcgcctcagg cacttcttct gcaagcatca taaatgggct gcgttgtacc agttcgcttt
     6601 ccgttacccg acgcgccagg tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc
     6661 ggctgctgat gttcgccaga tttttatcca gttgttcgcg cacatcagga cgaatacaag
     6721 agatatgaat atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa
     6781 ccgcgcgatc gggaaccggc tggccgtatt ttttgctcat aaaatcacgc gcctgccagg
     6841 ccaaccaaaa gaagttcggc gttgaaggat cggtcaacaa aggactttca gtaccgttaa
     6901 tacgatacgt tgacatcaac agatattgca gtggaccatt aagatctttt aaaaccacgt
     6961 atccggcatt gggtttgact tccgcacatg gcgaaggatt ttgattttgc tgctgattgg
     7021 gcaaacattc ctcaaggaca ctcttacgta atgtatccga ctcttcaccg gttaatttcc
     7081 agtaaccaat accggcagcc acaacggcga taactatcat caccaaaaaa agaagacccg
     7141 cttttttcat ctttttttcc ctgtacctca aagagatgca agggtaacgc aaaatcgtga
     7201 caaataaaaa acccggtcgc gagaaaccac cgggtttgat aattatccta gggaaatcag
     7261 cgtttgctga tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc
     7321 cagccgccga actcttcatc aatggtgaat aacttcagct ttggaaacgc attttcgtac
     7381 tttttcgcta cttcagcgtc gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct
     7441 tctggcgagt agagatattt caggtaggct tccgccacct ctttggtgcc ttttttctcg
     7501 accactttat cgaccaccga caccgttggc tctgcgagga tagactcact cggcgtgacg
     7561 atttcgaatt tatctttccc cagttcattc gctgccagca gagcttcgtt ttcccaggcg
     7621 atcagtacat cgccaatccc gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa
     7681 tccagaactt cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg
     7741 ttgttgttgt gatgcagcgc gtagccccag gcagccaggt agttccagcg tgcgccacca
     7801 gagcttttcg gattaggcgt gatcaccgaa acacccggtt taatcagatc gttccagtca
     7861 tggatctgct tcggattgcc cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg
     7921 gagttatccg gcagacgttt gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg
     7981 tccacgtcat aggccagagc cagcgtgaca acatcagctt caataccgtt gattaccgac
     8041 gtcgcttgtt tacctgaacc accgtgtgac tgacgaatca ccacgttatc accagtttgc
     8101 tgtttccagt gggcgctgaa tgccttgttg tactgttcgt acaattcgcg cgttggatca
     8161 tatgaaacgt taagaagctg aatatccttt gccataacgc tggttgccgc cagcaaaaat
     8221 gttaacccta cgccccactt gttcatcgcc cgactctctt atgttgtgtt gtgatgagca
     8281 aagcgtgcca gaaggttaac caaacattaa agaataaaaa aagattggct ataacttgcg
     8341 ggtatatgtt gagggattaa aaaggcggaa gaaatccgcc tcatattgct gacaaagtgc
     8401 gctttgtcca tgccggatgc ggcgcgaacg ccttatccgg cctacaaaag tttgcaaatt
     8461 caataaattg cagaattcat gtaggcctga taagcgaagc gcatcaggca tttttgcttc
     8521 tgtcatcggt ttcaggctaa aggaatctgc ctttttccga aatcattaat acagtttttt
     8581 cgcgcagtcc agccagtcac ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat
     8641 gtcgtggtga accagctgtt cgttctggat acctacacaa cgaccgccgt aacctgccag
     8701 cagcagatcg atagcgtaag cgcccatacg ggaagccaga atacggtcgt aaggcaccgg
     8761 agaaccaccg cgctggatgt ggcccagcac agttgcgcgg gtttcacgac cggtttcttt
     8821 ctcgatgaaa tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc
     8881 gtgtttttta cctttcgcga tacccgcttt gatttcgttt accaggtctt cacggctgaa
     8941 ttcaacttcc ggaactacaa cgaattcaca gccaccggca atggccgcag ccagggtcag
     9001 atcgccacaa taacggccca tcacttccac cacggaaata cgctggtgag aagaagaggt
     9061 gtcacgcaga cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga aaccgatagt
     9121 gtagtcagtg cctttgatgt cgttgtcgat agtgcccggc agaccgatgc acgggaagcc
     9181 catttcggtc agacgcattg cacccatgta ggaaccgtca ccgccgataa ccaccagcgc
     9241 gtcgatacca cgttttttca ggttttcgat agccacggcg cggatgttct cgtcgcggaa
     9301 ttccgggaaa cgcgcagaac cgaggaacgt accgccacgg ttgatcatgt cagacacgct
     9361 gtaacggtct agctgtacca tacggtcttc atacagaccc agatagccgt cataaatacc
     9421 cattacttcc agaccttctg tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat
     9481 gcctggcgca tcaccgccgc ttgtcaacac acctattttc ttaatcatga ctacctctga
     9541 actttggaat gcaaaatgaa atctgttgcc ggaagtcttc ttgcacatcg aagtgatcca
     9601 acgaatgtgc aaatagtata acaatcactt cctgctgaat tgattcaggt caggccaaat
     9661 ggcggtattt tatacacaaa atgcgggtct ggctctcttt tatactgatt atgaaagcat
     9721 agaccgttta ccctccctgg gtacgacgga acaggggtcc tgatggataa tcacatccga
     9781 tcccggaaaa cgccgtaaaa tagcctgctc tacctgatcc gccaccatat gtgcctgaac
     9841 caaaggcaga gagtcttcca tttccaaatg gatctgaata aagcgggtcg gccctgactg
     9901 ccgcgtgcga agatcgtgag cgccgctaac acccggccag gaagtcacga tatcaataat
     9961 ttcttgccgt tcttcatcag gcaatgcgcg atccagtaat gactgtaccg cctcatatcc
    10021 catgcgtaac gcgctatata aaatatagat gccgattccc aatgcaaaca gagcatcggc
    10081 gcgatgccag ccgtaccaag acaaccccag cgccagcaga attgcgccgt tcatcataac
    10141 atcagactgg taatgtagca tatcagcccg caccgcctgg ctttgcgtcc ggcgcaccac
    10201 ccaccgctga aacgagacaa ggataatcgt acaaattagc gccacaattg tcacgataac
    10261 cccgacgcct ggatctgtca tcggtgttgg agataccaga tgttgaatac ccgtcaaaaa
    10321 caggaatagt gccgaaccgg agataaacat actttgcgcc agcgccgcga gggactcagc
    10381 tttaccgtga ccaaacgagt gattatcgtc ggcaggttgc agggaatatc gcaccaccag
    10441 taaattcgtc aacgacgcgc cgatatccac cagcgaatcc accagcgcgg cgagaatact
    10501 caccgacccg gtataccacc atgcaaaaat tttaatcagc aatagcaacg aagccatcgc
    10561 cgtcgcagca atcgccgccc gactgaccag ccgtccataa gattgattca taaatactcc
    10621 cgctatcaac tgacgctagt ataacggaag caaatcatct gcaatgcatt aagcagcagg
    10681 caaattgagg ataaaaaaaa ccccacatca tgtgggggaa gacagggatg gtgtctatgg
    10741 caaggaaaac agggtttact actgggaacg tgagttgcta ctactcaata gcttcaacga
    10801 tgaacttttt tgccattgcg tcacgtcacg caactgctcc attcgttgtt gatgtttctc
    10861 gtttaaaacc gcttgctgct ccggcgttaa caggcgatac atttggttgc ggacttttgc
    10921 catctcaacc tgacgagcaa tttgctcatt cgccattttt tctgcctgtg cgcgcacagc
    10981 gttttcatca aaattttctg cggtgacaag gcgatgcatt gtctccagtt cgctaacatt
    11041 aacaggaggc tgttcgtgcc gggcctgttg cataagatct cgcatctgct gacgctgatg
    11101 ttcggttaaa cttatgccgt cgaacatatg gctctgcgta ctgcgctgcg taagttcttc
    11161 acccggatgc cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg aactgactgc
    11221 cagcgttgag gccatgacgg cagcggtaac tatgcgcatc atttgctccc aaaatctttc
    11281 tgtcgcgatt caacgataga gagtttacga ttcaggctgc aaacatgcgt cagggggtgt
    11341 aaaacaacgt aaagtcatgg attagcgacg tctgatgacg taatttctgc ctcggaggta
    11401 tttaaacaat gaataaaatc ctgttagttg atgatgaccg agagctgact tccctattaa
    11461 aggagctgct cgagatggaa ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc
    11521 ttgatcttct ggacgacagc attgatttac ttttgcttga cgtaatgatg ccgaagaaaa
    11581 atggtatcga cacattaaaa gcacttcgcc agacacacca gacgcctgtc attatgttga
    11641 cggcgcgcgg cagtgaactt gatcgcgttc tcggccttga gctgggcgca gatgactatc
    11701 tcccgaaacc gtttaatgat cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt
    11761 cgcactggag cgagcaacag caaaacaacg acaacggttc accgacactg gaagttgatg
    11821 ccttagtgct gaatccaggc cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa
    11881 ccggtactga gtttaccctg ctctatttgc tggcacagca tctgggtcag gtggtttccc
    11941 gtgaacattt aagccaggaa gtgttgggca aacgcctgac gcctttcgac cgcgctattg
    12001 atatgcacat ttccaacctg cgtcgtaaac tgccggatcg taaagatggt cacccgtggt
    12061 ttaaaacctt gcgtggtcgc ggctatctga tggtttctgc ttcatgatag gcagcttaac
    12121 cgcgcgcatc ttcgccatct tctggctgac gctggcgctg gtgttgatgt tggttttgat
    12181 gttacccaag ctcgattcac gccagatgac caagcttctg gatagcgaac agcgtcaggg
    12241 gctgatgatt gagcagcatg tcgaagcgga gctggcgaac gatccgccca acgatttaat
    12301 gtggtggcgg cgtctgttcc gggcgattga taagtgggca ccgccaggac agcgtttgtt
    12361 attggtgacc accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg
    12421 taactttatt ggtcaggccg ataacgccga tcatccgcag aagaaaaagt atggccgcgt
    12481 ggaactggtc ggtccgttct ccgtgcgtga tggcgaagat aattaccaac tttatctgat
    12541 tcgtccggcc agcagttctc aatccgattt cattaactta ctgtttgacc gcccgctatt
    12601 actgctgatt gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg cctggagtct
    12661 ggcaaaaccg gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg
    12721 ccagcacccg gaactggaag cggggccaca ggaattcctt gccgcaggtg ccagttttaa
    12781 ccagatggtc accgcgctgg agcgcatgat gacctctcag cagcgtctgc tttctgatat
    12841 ctctcacgag ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg
    12901 tcgtagcggt gaaagcaagg aactcgagcg tattgaaacc gaagcgcaac gtctggacag
    12961 catgatcaac gatctgttgg tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga
    13021 aaccatcaaa gccaaccagt tgtggagtga agtgctggat aacgcggcgt tcgaagccga
    13081 gcaaatgggc aagtcgttga cagttaactt cccgcctggg ccgtggccgc tgtacggcaa
    13141 tccgaacgcc ctggaaagtg cgctggaaaa cattgttcgt aatgctctgc gttattccca
    13201 tacgaagatt gaagtgggct ttgcggtaga taaagacggt atcaccatta cggtggacga
    13261 cgatggtcct ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt tctatcggac
    13321 cgatgaagca cgcgatcgtg aatctggcgg tacaggattg gggctggcaa ttgttgaaac
    13381 cgccattcag cagcatcgtg gctgggtgaa ggcagaagac agcccgctgg gcggtttacg
    13441 gctggtgatt tggttgccgc tgtataagcg gagttaaact ccgcatttgt aggcaggata
    13501 aggcgtttac gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct
    13561 tatcaggcct acactccggg ggttattttc cccacaaccg gcgggaaaaa tcttcgatct
    13621 tgccgctcag tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg
    13681 agagtaaacg gtgatactga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct
    13741 cctgcacggt cacatcgctg acacgggaaa ccaactccag cggcgcgtcc gcagacactt
    13801 tccctggcgc tatcacgcta tacaaccacc ccaccttacc tgtgttttgc atcaactgcg
    13861 ctatatcgct gatatcaaaa tggtaattga gtttatagca aggcgagcgc ggctggctga
    13921 cctgaattaa tgcctctccc cagcggaaaa tatcgcccat atgaacatta ctttccgtga
    13981 gaccgtcggt tgagagattt tcgccaaacg caggcgcaac aaacaactcc gcctgttccg
    14041 gaaattcccg cacccagtag agatagtgct cacgaggata atgacacagc gctctgtctg
    14101 gcccgccgtg aacctttttc tccgcctgct cgtcaccttc cagccccagc tccgtcagca
    14161 tcaactcacc atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct
    14221 gaatcttgcc tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt
    14281 tccccattag accatattta ttttatgtca gccgtgctgt tttcgcggta agtatccagg
    14341 caggaactca atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg attaaggaag
    14401 caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt gactttacgt
    14461 gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc cgtcgctacc
    14521 agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcaca
    14581 ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca
    14641 ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat tattgccaca
    14701 cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat ggtattgccc
    14761 agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa ttctcgtaat
    14821 tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa
    14881 acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt catgagcggc
    14941 ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc gtactgctgc
    15001 atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag cgccagcgtt
    15061 gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg cgaggcaatt
    15121 gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga tttacgcagt
    15181 accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca caccgccaga
    15241 atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt ccccgccccc
    15301 ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat cccccccggg
    15361 attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat ttttaagggc
    15421 tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag acattcattt
    15481 aatcaataaa agtgatcaaa accacatcaa ttgaaacgct gttttatttt tataggatca
    15541 ttgtttttat tttggaattg ttcacaaaaa aaccaccgcc gttggcgatg gttcattttt
    15601 taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt attttttcgc
    15661 cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt tgatgtagtc
    15721 cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca ggcccagaat
    15781 cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct ggttagcagt
    15841 agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg aaccaaagcg
    15901 ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga agtcacgttc
    15961 gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt tccagaacag
    16021 gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag
    16081 ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg gcaggctttc
    16141 cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt ggatttccat
    16201 ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg atggcagggt
    16261 atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt
    16321 tggtttatta tagttaatta aatgatattg aaaatgatta tcaatgccgc acttttcgta
    16381 agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac ttaatgccgt
    16441 gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt
    16501 tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag aagatgagta
    16561 acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt
    16621 acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca gtcggtggga
    16681 ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg gatttctggg
    16741 cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt
    16801 gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg tcgatgggaa
    16861 ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg
    16921 gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc ggcgttctgg
    16981 tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag cgcaagatgg
    17041 gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg atgtgcggca
    17101 ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat gaagccgctg
    17161 ccgcacttgg cgtcgatcca ctgtatgtcg ctctgccaag ctatgttgtc atcatgggcg
    17221 gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg aaggatttgt
    17281 cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg ttactctcgg
    17341 cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc cacgcccgca
    17401 ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat gtattgtgcg
    17461 gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt ccggtaacgg
    17521 tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc atcggcatgg
    17581 cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc ccggtttccc
    17641 gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc gaaatatcac
    17701 tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg acctgccgca
    17761 gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa acgcgctcac
    17821 tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg ctagccgcca
    17881 gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac gaatcactgg
    17941 tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca gccatttcgt
    18001 tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc tgccgcgcct
    18061 gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg ctaaatcccg
    18121 gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa ataatattct
    18181 gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga atataaaaga
    18241 gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca ttaccgcgcc
    18301 agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct tgcggataac
    18361 ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg agaagtttta
    18421 actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc gcaatatggc
    18481 gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc ctggcgaata
    18541 tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt actgtcgctg
    18601 aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag cagatgtcgg
    18661 gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt ttgctgctta
    18721 agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc atcccaattc
    18781 acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg tgatgcgctg
    18841 ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg cataaacaag
    18901 atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc catctgtgca
    18961 accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga cggatactgc
    19021 ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa tcgatccggc
    19081 gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag atgccgatca
    19141 tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc attaaacaca
    19201 tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg atgttcagga
    19261 aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga cggaaaaaaa
    19321 tccacactac gtaatacggt catactggcc tcctgatgtc gtcaacacgg cgaaatagta
    19381 atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa aaacgtcgat
    19441 ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca ccacaattca
    19501 gcaaattgtg agcatcatca cgttcatctt tccctggttg ccaatggccc attttcctgt
    19561 cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg aaattcagca
    19621 ggatcacatt atgacctttc gcaattgtgt cgccgtagat ctcggcgcat ccagtgggcg
    19681 cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg aaatccatcg
    19741 ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg atagcctgga
    19801 aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta tcgatagcat
    19861 tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc agcgtgtggg
    19921 cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg cacaacagca
    19981 actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct tcaatacgct
    20041 ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca ttgctcacgc
    20101 tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact gggaatatac
    20161 caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg agtcgctact
    20221 ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg gtaatgtcat
    20281 tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg ccagccatga
    20341 taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt atctctcttc
    20401 tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg acacggcgct
    20461 ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc tgaaaaatat
    20521 tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg atctcccggc
    20581 gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc ccaatgacga
    20641 tcgctttatc aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc gggaaacggc
    20701 gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca gtctggcgct
    20761 gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt tctcgcaact
    20821 gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg ccgatgcctg
    20881 cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata tcggcatcga
    20941 gttaattacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg tcagcaccac
    21001 cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact atgtggcgca
    21061 gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact ggaacaggcc
    21121 tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga ggcgctgcgc
    21181 caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt ttccggtttt
    21241 gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta tccgggcaaa
    21301 gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct gattccgggg
    21361 ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt ctcgcgcgac
    21421 cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa tcagctcggt
    21481 ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt tacgctctcg
    21541 catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag ccgtcgcgtt
    21601 tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg gatcccggat
    21661 ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct ggcagcactg
    21721 gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt tgagagcaaa
    21781 ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta catggggtat
    21841 gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc gactgaagtg
    21901 atttccgaca agatttccgc cgccatgctg tatgtgccgc agttgctgct gcacgtcagc
    21961 cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac ccaggcaatt
    22021 gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct tgacttcttc
    22081 gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat gaaaaaagcc
    22141 ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc ggcgggcgat
    22201 tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgtggca ggcggtctgg
    22261 gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga gagcgtgcgg
    22321 gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc ggcacaagcg
    22381 ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc cggccctctc
    22441 cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga acaccgctcc
    22501 ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag ccgcaccatg
    22561 tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta cccagtcctg
    22621 gtttgtccag ggaatgatca aagccaccac cgatgcctgg ctgaaaggct gggacgagcg
    22681 caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt atcacgacaa
    22741 cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac tggcaaatac
    22801 gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg atcctgcagc
    22861 taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc tctgggggtt
    22921 aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc actgcgagcg
    22981 cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca ccaacctgat
    23041 cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac tgtgggaagg
    23101 cagcaccgaa tgtctggcag tattcccgga tggcgttggc attctgccgt ggatggtgcc
    23161 cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt cgctggtgtt
    23221 gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct ttggtttaat
    23281 cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg gcggcatgaa
    23341 acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg ttacaccact
    23401 agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg tcatgcaggt
    23461 aaaccccgac gcccacgaag agtatcagcg tcggcataat cccatctggc cggaactgga
    23521 agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca aagcgcgtaa
    23581 tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg tagccagtac
    23641 tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta acccagataa
    23701 cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc ccaatgtgcc
    23761 ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca tattacatct
    23821 cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa tgaataaccc
    23881 attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg caaatctgac
    23941 ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat cacttaatcg
    24001 ctccaccatt acaattgatt aaaatatatt ttatggagtc cgattaatgg cagctcttac
    24061 tgcaagctgt attgacctga atattcaggg caatggcgct tattccgttc tgaag
//
LOCUS       DAJTYF010000053        23913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTYF010000053 DAJTYF010000000
VERSION     DAJTYF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.053"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..323
                     /locus_tag="OO590_002223"
     CDS             <1..323
                     /locus_tag="OO590_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6637579.1"
                     /translation="PANNEKYLAYLQQAATLGSSNAQAEIGYLYLIGKELPQNLPDAG
                     VWFKKAAAQGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKALNELE
                     HQTK"
     gene            376..2106
                     /locus_tag="OO590_002224"
     CDS             376..2106
                     /locus_tag="OO590_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637580.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWMKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(2166..2657)
                     /gene="yajQ"
                     /locus_tag="OO590_002225"
     CDS             complement(2166..2657)
                     /gene="yajQ"
                     /locus_tag="OO590_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6637581.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2825..3736
                     /gene="panE"
                     /locus_tag="OO590_002226"
     CDS             2825..3736
                     /gene="panE"
                     /locus_tag="OO590_002226"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6637582.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3699..4289
                     /gene="yajL"
                     /locus_tag="OO590_002227"
     CDS             3699..4289
                     /gene="yajL"
                     /locus_tag="OO590_002227"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6637583.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(4343..5791)
                     /gene="thiI"
                     /locus_tag="OO590_002228"
     CDS             complement(4343..5791)
                     /gene="thiI"
                     /locus_tag="OO590_002228"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6637584.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5997..6239
                     /gene="xseB"
                     /locus_tag="OO590_002229"
     CDS             5997..6239
                     /gene="xseB"
                     /locus_tag="OO590_002229"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6637585.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            6239..7138
                     /gene="ispA"
                     /locus_tag="OO590_002230"
     CDS             6239..7138
                     /gene="ispA"
                     /locus_tag="OO590_002230"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6637586.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            7163..9025
                     /gene="dxs"
                     /locus_tag="OO590_002231"
     CDS             7163..9025
                     /gene="dxs"
                     /locus_tag="OO590_002231"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6637587.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            9080..10054
                     /gene="yajO"
                     /locus_tag="OO590_002232"
     CDS             9080..10054
                     /gene="yajO"
                     /locus_tag="OO590_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6637588.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEENSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(10108..10626)
                     /gene="pgpA"
                     /locus_tag="OO590_002233"
     CDS             complement(10108..10626)
                     /gene="pgpA"
                     /locus_tag="OO590_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6637589.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10604..11581)
                     /gene="thiL"
                     /locus_tag="OO590_002234"
     CDS             complement(10604..11581)
                     /gene="thiL"
                     /locus_tag="OO590_002234"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6637590.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDVLSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11659..12078)
                     /gene="nusB"
                     /locus_tag="OO590_002235"
     CDS             complement(11659..12078)
                     /gene="nusB"
                     /locus_tag="OO590_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6637591.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(12098..12568)
                     /gene="ribE"
                     /locus_tag="OO590_002236"
     CDS             complement(12098..12568)
                     /gene="ribE"
                     /locus_tag="OO590_002236"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6637592.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGSAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12657..13760)
                     /gene="ribD"
                     /locus_tag="OO590_002237"
     CDS             complement(12657..13760)
                     /gene="ribD"
                     /locus_tag="OO590_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6637593.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13764..14213)
                     /gene="nrdR"
                     /locus_tag="OO590_002238"
     CDS             complement(13764..14213)
                     /gene="nrdR"
                     /locus_tag="OO590_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6637594.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14364..14903
                     /locus_tag="OO590_002239"
     CDS             14364..14903
                     /locus_tag="OO590_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6637595.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            15202..16086
                     /gene="tsx"
                     /locus_tag="OO590_002240"
     CDS             15202..16086
                     /gene="tsx"
                     /locus_tag="OO590_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6637596.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16263..16610)
                     /locus_tag="OO590_002241"
     CDS             complement(16263..16610)
                     /locus_tag="OO590_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6637597.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16739..17710)
                     /gene="secF"
                     /locus_tag="OO590_002242"
     CDS             complement(16739..17710)
                     /gene="secF"
                     /locus_tag="OO590_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6637598.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17721..19568)
                     /gene="secD"
                     /locus_tag="OO590_002243"
     CDS             complement(17721..19568)
                     /gene="secD"
                     /locus_tag="OO590_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6637599.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19596..19928)
                     /gene="yajC"
                     /locus_tag="OO590_002244"
     CDS             complement(19596..19928)
                     /gene="yajC"
                     /locus_tag="OO590_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6637600.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19951..21078)
                     /gene="tgt"
                     /locus_tag="OO590_002245"
     CDS             complement(19951..21078)
                     /gene="tgt"
                     /locus_tag="OO590_002245"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6637601.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDMHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(21133..22203)
                     /gene="queA"
                     /locus_tag="OO590_002246"
     CDS             complement(21133..22203)
                     /gene="queA"
                     /locus_tag="OO590_002246"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6637602.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22296..22869
                     /gene="acpH"
                     /locus_tag="OO590_002247"
                     /pseudo
     CDS             22296..22869
                     /gene="acpH"
                     /locus_tag="OO590_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(22874..>23913)
                     /gene="malZ"
                     /locus_tag="OO590_002248"
     CDS             complement(22874..>23913)
                     /gene="malZ"
                     /locus_tag="OO590_002248"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6637603.1"
                     /translation="DRHNLGTGGACHNSESPWRDWYLFSDDGTALDWLGYASLPKLDY
                     QSESLVNEIYRGEDSIVRHWLKAPWNMDGWRLDVVHMLGEAGGARNNMQHVAGITEAA
                     KETQSEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWGFLANTDISYDPQQIDAQ
                     TCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDIARLPLAVVWLFTWPGVP
                     CIYYGDEVGLDGKNDPSCRKPFPWQVEKQDTALFALYRRMIVLRKKSQALRRGGCQVL
                     YAEDNVVVFVRVLNQQRVLVAINRGEASEVVLPASPFLNAVQWQCKEGHGQLTDGILA
                     LPAISATVWMN"
BASE COUNT         6077 a         6268 c         5875 g         5693 t
ORIGIN      
        1 tacctgcaaa caatgaaaaa tacctagcat atctgcaaca agctgcaaca ctgggcagta
       61 gtaatgccca ggcggaaatt ggctatttat atctcatcgg aaaagaacta ccacagaatc
      121 ttcctgatgc tggtgtctgg ttcaaaaaag cagctgcaca aggtaatttt gccgcacatt
      181 tctatctggg aaggatgtat cagaatggtg acggcgttga gagaaatatg gaaaaagcca
      241 ggtttcatct atctaatgcg gcagaaggtg gaatagaacc tgcgcttaaa gcattaaatg
      301 aacttgaaca tcaaacaaaa taaaacgctc agggaccacg taaggttcat caaacagaaa
      361 aggatatagt ttgcgatgaa agcgaaatct actttgttat taatgatttt atctgttttt
      421 tattctcatc aggttttatc cagcgacccc actcagccgg atgtttattc tgtcgtcgag
      481 aaaaaactaa actctgcttt acctctggaa gaaaatacca ccttcagatc gcaggctgaa
      541 tggtttaatt tgcaatatga attattagta tctggctacc ctgaacgtgc ttacaattta
      601 ctgagcaaac ttgagacaga atcgaaaaac gctaaatatt atctggattt aactgcctcc
      661 agagtcgctg aagaagaagc accagagaca gccttaactt ttttatcaaa aattgggtta
      721 aataaaccgg ctactcttac atcctttgag agctatatca atagctggat gaaaagcaaa
      781 caacccgaaa aggctatgca gcttctcagc ctcgatgaga atgccctgaa ttattattta
      841 ccgactgtgt tagtcgcata tcagatcgtt cctgataagg caataagtat ttataattct
      901 gtatatggag ataatgttgt tataccctat cagcaagtca ccatgttact tacagttgcg
      961 gagggttatc aaacaaaagg cgatataaaa aacgccattc attatgccga taaggcgctg
     1021 aatatgtttg ataaagctat tgctgtagat agcagtcgtg aatcccatta tagtgatgag
     1081 tatttgaaac tcatgaatat ctacgccgcc aatggcaaca aagaaaaagc ggtggtttta
     1141 tctcaacgtc tacacaaagc cattaccgaa tctcaatcga attacttaag tactctggaa
     1201 ggaattttat tgttttatag aaataatgat atgcagcaag actatcaaaa cagcctttca
     1261 aattacatag tatttatcga tgaaattttt gccttttccc catcaacaag aagtgaatta
     1321 agcctcatta atttattaaa ttcattaaat gaggtggaat tgatgagaaa acgtctggcg
     1381 tttctgatga cagcaccaga atacgcttgt tatgatagtc agtattgtta tgaagacaaa
     1441 gtcagcgcat taaaaatcct gtatcgtcat catgacaatg agctaatgga caaatatttc
     1501 acgactcttc ttaaagagat cagtaatcaa ccattagaag actgggatat aataataagt
     1561 aatactggtg aaaaatttag tgaagtgggt ctaaccaaat atgctcaaca tctcgcatct
     1621 ttcgctgaag aattttatat aaaagagcaa aaatcacatt ctgaaagtga aatgagagga
     1681 ctattttcat cacttgccga attatatagt tttggtaatg atactgccag cgcaaaccga
     1741 gtgttccatc agcatgtccc tgccttcaat acagatcata tgattcaata ttttataaat
     1801 gccaaagaat ggaacaatgc cagggaatta ttgataaaag aggaaatgtt gggtatgcat
     1861 aatctgatat tgttggagaa tatttgtatg caaaaaaatg cagaatgcat ggcacacatt
     1921 acctttacgc ttaataaatt aacaacacaa ccagcaataa caaaaataga tagcgtgggt
     1981 aatgagcaac tttatcagat tggcaaaatc taccataagt tggaaattaa acctgagcca
     2041 gagcaacagg ttttaattca gtcgctttat gaccgggcat caggaagcgc ctctgcaact
     2101 caataaatgc atggtcaaaa gggccaccac ggtggccctg acaatcagta gctatatcca
     2161 gaaagttaat cgcggaagtt tttgaactgg aacggctgac cgagatcgcc accgcgcacc
     2221 atcgccatga cagcctgcaa atcatcacga gatttgccag tcacgcggat ctcatcgccc
     2281 tgaatttgcg cctgcacttt cagtttgctg tctttgatca tcttgacgat ttttttctga
     2341 gtggcgctct caatgccctg tttcagtttc gcttccacaa accaggtttt accgctatga
     2401 acgatatttt ccggtacatc cagtgaactg ccttcaatgc cgcgcttcag cagcttggca
     2461 cgcagaatat ccagcaactg attgacctgg aagtcggact cgctcaacac tttgatggtt
     2521 ttgctggcgt cgttcagctc aaatgaggct tcaacgttac ggaagtcaaa acgggactcc
     2581 acttcgcgac tcgcgttatc gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca
     2641 atatcgaaag atggcatctt ttcctctccc ttcatttttg atgcgaagca taatacctgc
     2701 aaagttaaat aaccaacagt cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa
     2761 cagcatgcac aattaaggga tagccttatt gaacacccgg agtggttgtg ggtgaggagg
     2821 aacaatgaaa attaccgtat tgggatgcgg tgccttaggg caattatggc ttacagcact
     2881 ttgtaaacag ggtcatgaag ttcagggctg gctgcgcgta ccgcaacctt attgtagtgt
     2941 gaatctggtt gagacagatg gttcgatatt taacgaatcg ctgaccgcca acgatcccga
     3001 ttttctcgcc accagcgatc tgctcctggt gacgctgaaa gcatggcagg tttccgatgc
     3061 cgtcaaaagc ctcgcatcca cactgcctgt aactacgcca atactgttaa ttcacaacgg
     3121 catgggcacc atcgaagaat tgcaaaacat tcagcagcca ttactgatgg gcaccaccac
     3181 ccatgccgcc cgccgcgacg gcaatgtcat tattcatgtg gcaaacggaa tcacgcatat
     3241 cggtccggca cggcaacagg atggcgatta cagttatctg gcggatattt tgcaaaccgt
     3301 gttgcctgac gttgcctggc ataacaatat tcgcgccgag ctgtggcgca agctggccgt
     3361 caactgcgtg attaatccac tgactgccat ctggaattgc ccgaacggtg aattacgtca
     3421 tcatccgcaa gaaattatgc aaatatgcga agaagtcgcg gcagtgatcg aacgcgaagg
     3481 gcatcatact tcagcggaag atttgcgtga ttacgtgatg caggtgattg atgccacagc
     3541 ggaaaatatc tcgtcgatgt tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta
     3601 tatcaatggt tttctcttac gccgcgcccg cgcgcatggg attgccgtac cggaaaacac
     3661 ccgcctgttt gaaatggtaa aaagaaagga gagtgaatat gagcgcatcg gcactggttt
     3721 gcctcgcccc tggtagtgaa gagactgaag ccgtcaccac gatcgacctc ttggttcgcg
     3781 gcggtatcaa agtcaccact gccagcgtcg acagcgatgg caacctggca attacctgct
     3841 cgcgcggcgt gaagctgctg gcggatgcgc cgctggtcga agtggctgat ggcgaatatg
     3901 acgtgatcgt gctgcctggt ggcattaaag gcacggagtg ttttcgcgat agcactctgc
     3961 tggttgaaac cgttaaacag ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg
     4021 cgccagccac cgtgctggtg ccgcacgata tctttccgat tggcaatatg accggcttcc
     4081 cgacgctgaa agacaaaatt cccgctgaac aatggcagga caagcgcgtc gtctgggatg
     4141 cacgggtaaa attgctgacc agccaggggc cgggtacagc tatcgacttt ggtctgaaaa
     4201 ttatcgacct gctggttggg cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg
     4261 cggcagggat ttataattat tacgagtagt gtcggatgcg gcaaacgtcg catctgacca
     4321 gatgcgacgt aaaaactaca aattacgggc gatatacctt cacattgtta aagccttgct
     4381 cgcgcagata gagcgcctgc agacggctca tcaccccgcg ctcacaccac agcagccagg
     4441 ttttgttctg gtcgagatcg ccaaatttgg tgctcagttt atagaacggc agagaaacca
     4501 catcaatccc ttcgactttc agtggcttat cttcctgttc atcgacagaa cggatatcga
     4561 gaatcacgtc gttcgggccg aagccgttga cggtttccac ttccaccact tcctgctcgg
     4621 tctgctgggc gatttcgcgg atatcaacgt tattcgcttc ctcaaccact ttatcgagaa
     4681 tactgaagtc gaacttctct tcttccgctt caatcttcga tttaaccgct ttcaccgtcg
     4741 ggcttttgga gatcacgcca caatattccg gcatcgtgcg agcaaagtct tcggtgccaa
     4801 tctggcgagc caggttgatg atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca
     4861 gcgtgtcaga gacgttatca atcaggcgca ggttggtcag cgtctgactg gacacctggc
     4921 cgagcgcttc gccggtgacc agcgcctgta cgccgtaacg ttcagccact ttagatgcgg
     4981 cacgcaccat catacgtttg aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa
     5041 tttccccgac aaccggttcg aaattaatgg cgacaaaacg cacgcggtgg gagctgccaa
     5101 agcggttcca cagataatgc gccacctgac gcacgccaat ttcatgcgct gcgccgccga
     5161 ggttaaagaa gcagtaatgc acgcggcagc cgcgacgcat caacatataa ctggaaacac
     5221 cggagtcgaa accaccggaa atgagcgaca gcacatcttc ctgggtgcca atcgggaaac
     5281 caccaatacc ttcgtagcga cctttaatca gcaggaggcg atcgtcttcc acttccagat
     5341 gaaccgttac atccgggtta gtcagcttca cgcgcgcgga ttcaatatgc tgatttaaac
     5401 cgccgccgac gtaacgttcc acatcaatcg agctaaaatc atgtttgcca cggcgcttca
     5461 cgcgtacgca gaaggttttg ccttccagct gatcgcgata ctgaaccaac gctttctcaa
     5521 aaatatcgtg catgtcggta aacggcacgt cttcgacttc gagaatatgg tggatacccg
     5581 gaatacgggt cagggcgtcg cgaatagtca gacgctggtt ttcatctttc gcgcgaactt
     5641 cgatgttatc ccagtggcgg acgacagcga gcgtctcatc atagtgcttt aaaacgttac
     5701 gaatgttccc ggtaaggatt tttataaagc gcaagcgcac agattggctt ttgatggtga
     5761 tttccgggaa caatttaatg ataaacttca tggcggcaat ggttcgttgg caagccttaa
     5821 gcgacttgta tagggaaaaa tacagcagcc cacacctgcg gctgcatcca ggcgcggaag
     5881 tataccacta acatcgcttt gctgcgcaca tcaccttacc attgcgcgtt atttgctatt
     5941 tgccctgagt ccgttaccat gacggggcga aaaatattga gagtcagaca ttcattatgc
     6001 cgaagaaaaa tgaggcgccc gccagctttg aaaaggcgct gagcgagctg gaacagattg
     6061 taacccgtct ggaaagtggc gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg
     6121 gcgtgcagct ggcacgtcag gggcaggcca aattacaaca agccgaacag cgcgtacaaa
     6181 ttctgctgtc tgacaatgaa gacgcctctc taaccccttt tacaccggac aatgagtaat
     6241 ggactttccg cagcaattcg aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt
     6301 tatcgcccca ctgccctttc agaacactcc cgtggtcgaa accatgcagt atggcgcatt
     6361 attaggtggt aagcgcctgc gacctttcct ggtttatgcc accggtcata tgtttggcgt
     6421 tagcacaaac acgctggacg cacccgctgc tgccgtagag tgtatccacg cttactcatt
     6481 aattcatgat gatttaccgg cgatggatga tgacgatctg cgtcgcggtt tgccgacctg
     6541 ccatgtgaag tttggcgaag caaacgcgat tctcgctggc gacgctttac aaacgctggc
     6601 gttctcgatt ctaagcgatg ccaatatgcc ggaagtgtcg gatcgcgaca gaatttcgat
     6661 gatttctgaa ctggcgagcg ccagcggtat tgccggaatg tgcggtggtc aggcattaga
     6721 tttagacgcg gaaggcaaac acgtacctct ggacgcgctt gagcgtattc atcgtcataa
     6781 aaccggcgca ttgattcgcg ccgccgttcg ccttggtgca ttaagcgccg gagataaagg
     6841 gcgtcgtgct ctgccagtac tcgacaagta cgcagagagc atcggccttg ccttccaggt
     6901 tcaggatgac atcctggatg tggtaggaga tactgcaacg ttgggaaaac gccagggtgc
     6961 cgaccagcaa cttggtaaaa gtacctaccc tgcacttctg ggtcttgagc aagcccggaa
     7021 gaaagcccgg gatctgatcg acgatgcccg tcagtcgctg aaacaactgg ctgaacagtc
     7081 actcgatacc tcggcactgg aagcgctagc ggactacatc atccagcgta ataaataaac
     7141 aataagtatt aataggcccc tgatgagttt tgatattgcc aaatacccga ccctggcact
     7201 ggtcgactcc acccaggagt tacgactgtt gccgaaagag agtttaccga aactctgcga
     7261 cgaactgcgc cgctatttac tcgacagcgt gagccgttcc agcgggcact tcgcctccgg
     7321 gctgggcacg gtcgaactga ccgtggcgct gcactatgtc tacaacaccc catttgacca
     7381 attgatttgg gatgtgggac atcaggctta tccgcataaa attttgaccg gacgccgcga
     7441 caaaatcggc accatccgtc agaaaggcgg tctgcacccg ttcccgtggc gcggcgaaag
     7501 cgaatatgac gtattaagcg tcgggcattc atcaacctcc atcagtgccg gaattggtat
     7561 tgcggttgct gccgagaaag aaggcaaaaa tcgccgcacc gtctgtgtga ttggcgacgg
     7621 cgcgattacc gctggcatgg cgtttgaagc gatgaatcac gcgggcgata ttcgtcctga
     7681 tatgctggtg gtcctcaacg acaatgaaat gtcgatttcc gaaaatgttg gcgcgcttaa
     7741 caaccatctg gctcagctgc tttccggtaa gctttactct tcactgcgcg aaggcgggaa
     7801 aaaagttttc tctggcgtgc cgcccattaa agagctgctc aaacgtaccg aagaacatat
     7861 taaaggcatg gtggtgcctg gcacgttgtt tgaagagctg ggatttaact acatcggccc
     7921 ggtggacggt cacgatgtgc tggggcttat caccacgcta aagaacatgc gcgacctgaa
     7981 aggcccgcag ttcctgcata tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa
     8041 agacccgatc actttccacg ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa
     8101 aagtagcggc ggtttgccga gctattcaaa aatctttggc gactggttgt gcgaaacggc
     8161 agcgaaagac aacaagctga tggcgattac tccggcgatg cgtgaaggtt ccggcatggt
     8221 cgagttttca cgtaaattcc cggatcgtta cttcgacgtg gctattgccg agcaacacgc
     8281 agtgaccttt gccgccggtc tggcgattgg tggatacaaa cccattgtcg cgatctactc
     8341 caccttcctg caacgcgcct atgatcaggt gctgcatgac gtggcaattc aaaagctacc
     8401 ggtcctgttc gccattgacc gcgcgggcat tgttggtgct gacggtcaaa ctcaccaggg
     8461 cgcttttgat ctctcttacc tgcgctgcat accagaaatg gtcattatga ccccgagcga
     8521 tgaaaacgaa tgtcgccaga tgctctatac cggctatcac tataacgatg gcccgtccgc
     8581 ggtgcgctac ccgcgcggca acgcggttgg cgtggaactg acgccgctgg aaaaactacc
     8641 aattggcaaa ggcattgtga agcgtcgtgg cgagaaactg gtgatcctta actttggtac
     8701 gctgatgcca gaagcggcca aagtcgccga atcgctgaac gctacgctgg tcgatatgcg
     8761 ttttgtgaaa ccacttgatg aagcgttaat tctggaaatg gccgccagcc atgaagcgct
     8821 ggtcaccgta gaagaaaacg ccattatggg cggcgcaggt agcggagtga acgaagtgct
     8881 gatggcccat cgtaaaccag tacccgtgct gaacattggc ctgccggact tctttattcc
     8941 gcaaggaact caggaagaaa tgcgcgccga actcggcctc gatgccgccg gtatggaagc
     9001 caaaatcaag gcctggctgg cataatccct actccactcc tgctatgctt aagaaattat
     9061 tcatggcagg agtggcagca tgcaatacaa ccccttagga aaaaccgacc ttcgcgtttc
     9121 ccgactttgc ctcggctgta tgacctttgg cgagccagat cgcggtaatc acgcatggac
     9181 actgccggaa gagaacagcc gtcccataat taaacgcgcg ctggaaggtg gcataaattt
     9241 ctttgatacc gccaatagct attccgatgg tagcagcgaa gagatcgttg gtcgcgcact
     9301 gcgggatttc gcccgtcgtg aagacgtagt cgttgccacc aaagtgttcc atcgcgttgg
     9361 tgatttaccg gaaggattat cccgtgcaca aattttgcgc tctatcaacg acagcctgcg
     9421 acgtctcggc atggattatg tcgatatcct gcaaattcat cgcggggatt acaacacgcc
     9481 gatcgaagag acgctggaag ccctgaacga cgtggtaaaa gccgggaaag cgcgttatat
     9541 cggcgcgtca tccatgcacg cttcgcagtt tgctcaggcg ctggaactac aaaaacagca
     9601 ctgctgggcg cagtttgtca gtatgcagga tcactataat ctgatttatc gcgaagaaga
     9661 gcgcgagatg ctgcctctgt gttatcagga gggcgtggcg gtgattccgt ggagcccgat
     9721 ggcgcggggg cgactgacgc gtccatgggg agaaactacc gcacgactgg tgtctgatga
     9781 ggtggggaaa aatctctata aagaaagcga tgaaaatgac gcgcagatcg cagagcggtt
     9841 aacgggcgtc agtgaagaac tcggtgcaac acgagcacaa gttgcgctgg cctggttgtt
     9901 aagtaaaccg ggcattgccg caccgattat cggaacttcg cgggaagaac agcttgatga
     9961 gctgctgaac gcggtggata tcactttgaa gccagaacag attgccgagc tggaaacgcc
    10021 gtataaaccg catgcggtcg taggatttaa ataagagtcc aggcctgatg agacgtgaca
    10081 agcgtcacat caggcatcgg tgcacaacta cgacagaata cccagcggcc agtgatgacc
    10141 gataaagtac aggatgcctg cggaaatcac cccggcgaca atatcgtcga tcatgatccc
    10201 catgccgcca tgtacattgc gatcaaacca gcggatcggc cacggcttcc acatatccag
    10261 aatacggaaa atcacaaacc cggcggcaac ccactgccag tcattggtcg gcagcgccat
    10321 gagcgtgatc cacataccaa taaattcgtc ccagacaatg ctgccgtgat cgtgcacgcc
    10381 catatctttc gccgtttgat gacaaagata gacgccgata cagatcccca acatcaccac
    10441 cagcgagtag agctgccagg gtaaaaaggt catcagatac cagaaaggga tcgccgccag
    10501 cgatcccatc gtgccaggaa cgattgggct taatccactt ccgaatccga cagcaagcag
    10561 atgccacgga ttactcatct tcaggcgact tttcgcgaca tctttatggc gtggcaaaat
    10621 ggtcatatcc tttccagtca aacgtgacag gttcgccgtc acgaataaaa caaagccctt
    10681 cgatatcggc ggtcatttgc ccgatacagg taaacggtac gccaaggtgt ccgagagcaa
    10741 catccagcgc gccacggttc agttccggca ccgtgaaaca caactcgtaa tcttcaccgc
    10801 cagagagcgc ccagcgcagc gcctgttccg gttcaacatg gcgagaaagc acatcagaaa
    10861 acggcagcaa tgccaggtca atacgtgcgc cgcagtcgct ggctttcacg atatgcccga
    10921 gatcggaaat cagaccgtca gagagatcga tagctgaatt tgccagatcg cgcagtgcct
    10981 gcccctgtaa aatacgcggc gatggacgga gatgacgttt gatcaagtag tcagcatctt
    11041 tagcatcggc aacctgcaaa cggttttgca aaatcgccag cccggcggcg ctatcgcccg
    11101 gtgtaccggt cacatagatc cagtcacccg gtttcgcccc agagcgcgtt aaggctcgtc
    11161 ccatcggaac aaagccgtgg atacccaacg tcattgataa tggcccacgc gtggtatcgc
    11221 cgccaatgag ttgcatatcg taataattga gaagatcaaa caaactgtcg ctgaaggact
    11281 caagccacgc ttcgtctacg tccggtaagg ttaatgccag cgtcagccag gccggatcgg
    11341 cccccatcgc tgccagatcg cttaggttca ccgccagtgc tttataagcc agatcagcag
    11401 gatcgatatc agggaggaaa tggttgcccg ccaccagcgt atcagtgctg atcgccaggg
    11461 tctgtttctc ggggatattg agaagagcgc aatcgtcgcc gatgcccagt tcgacatcaa
    11521 gacgagaact tcttacacgg tcaaaataac gggcaatcag ggagaactcg ccacatgcca
    11581 tacgttatgc ctcagcaggt aaaaaagaaa aggccggcga tcatggaaca gtcttccgtg
    11641 aatctaccgg cctggatatt actttttgtt agggcgaatc acaggtgctg ctttatcgag
    11701 tacgccgttg acgaacttat ggctgtcttc tgcgccgaac gatttcgcca gttcgatcgc
    11761 ttcgttaatg gccactttgt atggcacatc actacgttta gacagttcgt acagcgcaat
    11821 gcgcagtact gctttttcta cctggcccag ttcttccagc aggcgggaca ggtagggctt
    11881 catcagtccg tcgaggtatg cggtgttagt cgccaccccg gccagcagct cacggaagta
    11941 cagaacgtca acgtctttta catcctgttc agccaggaac tggtattcaa catcagcgat
    12001 gtcgttctgg gacaactgcc aggagtagag cgcctggacg gcacactcac gagcgcggcg
    12061 acgagcagca ggtttcacgg atttcccctt actaatttca ggccttgatg gctttcaata
    12121 cattaatcat ttcaagcgcg gtcagtgcag cttctgcacc tttgttgcca gctttggtgc
    12181 cagcacgttc gatcgcttgt tcaatgcttt cagtggtcag aaccccaaaa gcaaccggaa
    12241 tttcgctgtc ctgggcaaca tgcgccaggc cgttgcttgc accaccagcg acatattcaa
    12301 agtgggcaga gccaccacga ataaccgtac ccagcgcaat caccgcgtcg tatttaccgg
    12361 ttttagccag tgcacccgcc gccagcggca gctcataggc accaggcacc caaacaacgg
    12421 taatgttttc atcttttacc tgaccgatac gtttcagtgc gtcaattgca ccttccagca
    12481 ggctgtcatt gataaagttg ttgaaacgcg cgatggtgat ggcgacgcga gcgtccgggg
    12541 tagcaacgtt agcttcaata atgttcattt tcttccttcg ggttcgagta tggccccgca
    12601 ggggggcgga ttttagcata atatttcgtg cgctgcttcc ctttcgagcc gggagatcat
    12661 gcccccacta aatgcaggca aacatccggg cctacatgac gtatctcttt gaatttaaat
    12721 tggggggcgt cggctaattt ctcaagccct ggcagcgagc ataatccacg ggcgtcgctg
    12781 cctaatagtt taggtgcgat atagacaatc agttcatcga ctaaacccgc ctgtagcaat
    12841 acgccagcga gcgttggccc cgcttccacc cagatgctgt taatctgctg tttacccagt
    12901 tgcatcatca gtacaaccag atccagatga cctttatgct ctggaatcag caaggtacgc
    12961 accgtttccg gccactcacg agaatcttcc tgcgtacgcg cgaaccaggt ttcgccgggc
    13021 tgctgcacaa tgcgatgttc cggcgtcacg cgattttggc tatcaatcac aatacgtact
    13081 ggctggcgga gattttgttg cgggtagagc gcctgagttt gttcatccag ttcagaccaa
    13141 cgcactgtta aggctggatc atccgccagc accgtggcgc tgctggttaa aatggcatga
    13201 ctttgtgcgc gctgtcgttg tacgtcgcgc cgcgcctgag gcgaagtgat ccactggctt
    13261 tcgccgctcg ccatcgccgt gcggccatca agcgatgcgc caagttttaa ctgaatataa
    13321 ggaaagccgg tgcgcatccg cttgagaaag cctttattca attgctcggc ttcactcatc
    13381 atcagcccgt ggctgacgtc aatgccagcc tgttgcagac ggtaaagtcc acgcccagcg
    13441 acctgcgggt taggatcttg catcgctgcg accacgcgcg ctacgccagc ggcgatgagt
    13501 gcgtcacagc acggtggcgt acggccatga tggctacagg gttcgagtgt cacatacgcc
    13561 gttgcgcctt tggctttttc acccgccata cgcaacgcgt gtacttcggc atgtggttca
    13621 cccgctcgtt ggtggtaacc ttcaccgaca atttcgccat ctttgacaat gacgcacccg
    13681 acattcgggt tggggtgggt ggtaaaacgt cctcgttgcg ccagctttag cgcccgcgcc
    13741 atgtaatact cgtcctgcac ggcttagtcc tccaggcgcg cgatctcttc gccaaattct
    13801 ttgatatctt cgaaactgcg gtagacagag gcaaaacgga tataggcgac tttatcgagc
    13861 tttttcaatt gctccatcac cagattgcca atcatcttgc tcggtacttc gcgctcaccg
    13921 gtggcgcgca gctgcgattt aatatgattg attgccattt caacgtaatc ggaactcacc
    13981 ggacgttttt ccagcgcccg cagcattccg ctacgcaatt tctcttcatt aaacggttca
    14041 cgcacgtcgt tgctttttac aacacgcggc ataaccagct ccgccacttc aaaggtggtg
    14101 aaacgttcat tacacaccag acactgccga cggcggcgta cggatgaacc ctcgcccacg
    14161 agacgagagt caattacctt agtgtccacg gcgaaacaga atgggcaatg catacggcgt
    14221 cctgaccagg tggttaacgg aaatctattt taccctgaac tgacgataca acaaaggcgc
    14281 aacgcttttg agataaagga tcgatgcttt cgctgctttt accctctacc attaggctaa
    14341 ttgcgacgag aaagggacga cctatgaata ctaacgtttt tcgactgctc ctgctgggaa
    14401 gcctgttcag ccttagcgcc tgtgtgcagc aaagtgaagt gcgacagatg aaacacagcg
    14461 tcagcacgct gaaccaggag atgacgcaac tcaatcaaga aacagtcaaa atcactcagc
    14521 aaaacaggct gaatgcaaaa tccagcagtg gggtttacct tctgcctgga gcgaaaacac
    14581 cggcaagact ggaaagccag atcggtactt tacgtatgtc gctggtgaat attacgcctg
    14641 atacagatgg caccaccctt acactacgta ttcagggtga gtcgaatgat cccttgcccg
    14701 cattcagcgg aactgttgaa tatgggcaaa ttcagggaac aatagacaac tttcaggaaa
    14761 tcaatgtgca gaatcaattg attaatgccc ctgccagcgt cctcgccccc agcgatgttg
    14821 atattccgtt acaattaaag gggatttctg tggatcagtt aggctttgta cgtatccacg
    14881 acattcaacc tgttatgcag taaacgtatt ttgggacgat tttgtgcgtc ccgcaacatc
    14941 tttccccgtc attttgttac tctgcttaca tcacttggat tgatagtaaa agtttgcaac
    15001 aagggcgaaa gtcagtacaa tccccgcccg aatgtgtgta aacgtgaacg caatcgatta
    15061 cgtaaatgat agaactgtga aacgaaacat atttttgtga gcaatgattt ttataatagg
    15121 ctcctctgta tacgaaatat ttagaaacgc aatttgcgcc tttttcactc ccgcaaggga
    15181 ttttcaaaca gtggcataca tatgaaaaaa acattactgg cagccggtgc ggtactggcg
    15241 ctctcttcgt cttttactgt caacgcagct gaaaacgaca aaccgcagta tctttccgac
    15301 tggtggcacc agagcgttaa cgttgtcgga agctatcaca cccgtttcgg accgcagatc
    15361 cgcaacgata cctaccttga gtacgaagca ttcgctaaaa aagactggtt cgacttctat
    15421 ggttatgcgg atgcgccggt attcttcggc ggtaactccg atgctaaagg tatctggaac
    15481 cacggttctc cgctgtttat ggaaatcgaa ccacgtttct ccatcgacaa gctgaccaat
    15541 actgacctta gcttcggtcc gttcaaagag tggtacttcg cgaacaacta catttacgac
    15601 atgggtcgta ataaagatgg tcgccagagc acctggtaca tgggtctggg taccgatatc
    15661 gacactggcc tgccgatgag cctgtccatg aacgtctatg cgaaatacca gtggcagaac
    15721 tatggcgcag cgaacgaaaa cgagtgggac ggttaccgtt tcaaaattaa atactttgtg
    15781 ccgattaccg atctgtgggg cggtcagctg agctacatcg gcttcaccaa cttcgactgg
    15841 ggttccgatt taggggatga cagcggtaac gcaatcaacg gtattaagac ccgtactaat
    15901 aactctatcg cttccagcca tattctggct ctgaactacg atcactggca ctactctgtc
    15961 gtagctcgtt actggcacga cggtggtcag tggaacgacg atgcagaact gaacttcggc
    16021 aacggcaact tcaacgttcg ctctaccggc tggggtggtt acctggtagt aggttacaac
    16081 ttctgattat gaaaatgccg ggatttattc ccggcatttc tgattgttaa caacatgtgc
    16141 tttgttcatg ccggatgcgg cgtaaacgct ttgtccggcc cacaaaagca cgtaaattca
    16201 atatattgct gaaataacgt aggcctgata agcgtagcgc atcaggcaat tttacgtttt
    16261 aatcacttct tcttgttcat catcgctttc agatcagcga atgggttgta cttcgcttca
    16321 ccgacatctt tctgcgcatc ttcccctgcg ataacggtcg taccatactg atccgcttcg
    16381 gtatatttcg aatgctcatg atcgtggcaa tagagacaca acaattccca gttactgcca
    16441 tcttccgggt tattggtatg gtcgtgatca atgtggtgaa cggtaagttc acgcaggtta
    16501 gaataaacaa actcgcggga acagcgaccg cagacccacg gatagatttt taatgctttt
    16561 tcgcgatagc cgctttctaa ccgtgcatag ttttttggga tgatagccat gaaaagttac
    16621 ctggatgaaa tgtagggtta cgcgcagttt cccaagatgc ggggattttc gcaatctcca
    16681 tacatcaaaa aaaatcccga tcttctgacc gggattttca acatcaacgg gaacttgatt
    16741 aaggcagaat tgacggctga tccgcccctt ctttttccac tttctgctgc aacatgtgtt
    16801 cgcgcttcat acccagtttc agagccaacg cagatgctac gtagatggaa gatgcagtgc
    16861 cgatggaaac accgataagc atggtcagcg agaagccttc cagtaccgga ccaccgaaga
    16921 gatacagcat caggataacc atcaaggtag taccggatgt gatcaaggta cggtgcagcg
    16981 tctgggtcaa ggacacgtta aagatttcgt aaggcgtacc gcgacggatc ttgcggaagt
    17041 tttcacgaat tcggtccgat accacgatac tgtcgttaag cgagtaaccg ataaccgaca
    17101 tcaacgatgc cacaatggtc aggtcaatct cgatatggaa taacgacaaa atacccagcg
    17161 taatgataac gtcgtgcgcc agcgcaataa ccacccctgc cgccagtcgc cactcaaagc
    17221 ggaaacctac gtacacgagg atagacagca gcgctgccat caacgccatc gcaccggttt
    17281 gcgcaaggtc tgcccccacg ctcggaccga cgaactcaat acgcttcact gctgcattct
    17341 gattggtgga ttcgttaatc accttcagaa cttggctgcc cagcacctga ccgccggttt
    17401 cgccttcagc aggcggcata cggaccatga tgtcgtggct gctaccaaag ttttgcagca
    17461 ttggctcttc aaaaccggct ttttgcaatg catcacgcat tacgtcaatt tcagccggtt
    17521 tttcgagcgt aatttcgata accgtaccac cggtgaaatc cagcccccag ttaaagccgc
    17581 gcacgcccat aataacgata gcagcgatta acagcagacc agagatgccg aaagcccagt
    17641 agtcccagcg cataaagtca tagactttac ggccgtggtt tagttgttca acagtatatt
    17701 cctgtgccac atcgcactcc tcagattgac agcttcttga cgcgtttgcc gccatatagc
    17761 aggtttacga tggcacgcgt accgacaatc gcggtaaaca tcgacgtcgc cacaccgata
    17821 ccggtagtaa tcgcgaaccc tttaattgcc ccggtaccca ctgcgtacag gatgatgact
    17881 ttaatcagcg tggtgatgtt cgcatcgaag atagaactga atgcgccacg ataaccttca
    17941 tcaattgcct gttgaacagt acgtccgttg ctcaactctt ctttaatacg ttcgttgatc
    18001 agtacgttcg catcgaccgc taccgcaagg gttaagacga tacccgcgat acctggcata
    18061 ctcagtgttg cgcctggcaa cagcgacata ataccgacga tcaagatcaa gttggcaatc
    18121 agagcactgg tcgcaatcag accaaacttc ttatagaaga tgatcatgaa cagaatagaa
    18181 accagcagac cggcaaggca agcctccagc ccctgctcaa tgttctgcat acccagggtc
    18241 gggccaattg tacgttcttc aacaatctga atcggcgcga tcaacgcacc ggcacgcagc
    18301 agcagtgaca gctgacgggc ttcgttcggg ttgttgatgc cggtgatacg gaagctgtta
    18361 cccagacgag actggatgtt ggcgatgtta atcacctctt cctgtttcac cagaaccgca
    18421 cgaccatttg catctttctt accgctgtct ttgtactcca caaacagggt tgccatcggt
    18481 ttgccgatgt tgtccttggt gaagttagac ataatgttgc caccagcgct atcgagcgag
    18541 atgttaacct gcggctggtt gtattcgtcc tggctggaag tggagtcggt gatatggtca
    18601 ccagtcagaa ttacgcgttt gtacagcaca accggctgac cttcgcgggt ctgtttcact
    18661 tcagagtcgc ccggtacgcg accggatgcc gcagcggcct gatcaacatt ggtgtttacc
    18721 agacggaatt ccagcgttgc cgtcgcgccc agaatctctt tcgcacgcgc agtgtcctga
    18781 ataccaggca gttcaacaac gatacggtca gcaccctgac gctgaaccac cggctccgcc
    18841 acgccaagtt ggtttacacg gttacgcagg atattaatgt tctgctgcac cgcatattca
    18901 cgcgcttcac tcagacgagc atcgctcatt accgcacgca gctggttgct gccctggctg
    18961 ctaatcacca ggtccggatg gcgcttgctc agatacgcaa tggcttcatc acgagcttta
    19021 gcatcgcgga aagtgatgct caggccgtag ttgttttctt tacgaacagt ggtatacggg
    19081 atgccctttt cgcgcaggtc actgcgtagg ctatcgatat tttgttcctg gagtttgcca
    19141 agcgcggtgt ccatatccac ttccatcagg aagtgaacgc cgccacgcag gtcaaggccg
    19201 agcttcatcg gctcagcatg aatagctgcc agccagcgcg gcgttgccgg ggcaaggtta
    19261 agcgccacga cgtatttgtc acccataacg cccattaatg cttcacgagc gcgcaactgg
    19321 gtgtcagtgg agtcgaagcg cgcaagaata gcgccctctt ccagtgccac agacttagca
    19381 gttatttttt cttcttgtaa cgttttctgg acctggatca gcgtttgctc actggcggcg
    19441 actccgcgcg caccagtgat ctgaacagcc ggatcctcac caaacaggtt gggaagcgca
    19501 tacagcagac cgatgacaat caccacgatc agcatgacgt acttccacaa aggataacgg
    19561 tttaacacgg caattccctt agggaaaaat tttaattaca gcgccttcat ggtgcctttc
    19621 ggcaggacgg cagctacgaa gtcacgttta ataactactt cagtggtgtc attcagcgcg
    19681 atagcaatgt agccgttttc cgctactttg gttacgcgac caaccaggcc accgttcgtc
    19741 agaacttcat cacctttggc aatggagtcc atcagctttt tgtgttcttt ggtgcgcttc
    19801 tgctgtggac gcaggatcat gaaatagaaa atcagaccga ataccaccag catcaaaatc
    19861 aaagacatcg ggctaccttg cgccggtgca cccgttgccg ctaccgcatc agaaataaaa
    19921 aagctcatta aatttccctc attattaata ttaatcaacg ttcaaaggtg gtacttctcg
    19981 cccctgacgc tggtaaaaat cagttacgaa gctctctaat ttaccctctt caatagcctt
    20041 gcgtaaaccc gccatcaaac gctggtagta acgaaggtta tgaatggtgt tgagtcgcgc
    20101 gcctaatatt tcgttgcaac ggtcgagatg atgcaagtaa gcgcgtgaat aattgcgaca
    20161 ggtgtagcaa tcacactcag gatcgagtgg gccagtatcg ctcttatatt tcgcattgcg
    20221 gattttcacc acgccatcgg tcacgaacaa atgaccattt cgggcgttgc gggttggcat
    20281 tacgcagtca aacatatcga taccgcgacg tacgccttca accaggtctt ccggtttacc
    20341 aacgcccatc aggtaacgcg gtttgtctgc cggaatttgc ggacacacat gctccagaat
    20401 acggtgcata tctgctttcg gctcacccac agccagaccg ccgacagcgt agccatcaaa
    20461 gccgatatct accagacctt taacagatat atcacgtaaa tcttcgtaaa tactgccctg
    20521 aattatacca aataacgcat ttttgttacc aagactgtca aaacgctcac ggctacgctt
    20581 cgcccaacgc agagacatct ccatagagcg ttttgcgtaa tcccagtcag caggatacgg
    20641 cgtacactca tcaaagatca tgacgatatc cgaaccaaga tcgtactgaa tctccatcga
    20701 cttttccggg tcgaggaaaa tcgggtcgcc gttgatcggg ttacggaagt gaacgccctg
    20761 ttcggtgatt ttacgaatat caccaaggct gaagacctgg aagccgccgg agtcggtaag
    20821 aatcggtcct ttccactgca taaaatcgtg catatcgccg tgcagtttca tgatttcctg
    20881 gcccgggcgc agccacaggt ggaaagtgtt gccgaggata atttgcgcgc cagtggcttc
    20941 aacttcttcc ggcgtcatcc cttttacggt gccgtaggtg ccaacaggca taaaacaagg
    21001 cgtttccact acgccacgat caaagaccag gcggccacgg cgtgcgcgac cgtcggtggt
    21061 gtccagttca aatttcattt tttctccact acgtcagaaa aacagtccaa cgtttaaacc
    21121 agcgccgcgg aattactccc cgacgcgctc attaattgcc tgcggattgt acgtgataaa
    21181 catcgcatca ccgtaactaa aaaagcgata tttctcttct accgccgctt tataggcatt
    21241 catggtgtgt tgataaccgg caaaggccga aaccagcata atcagcgtcg actctggcaa
    21301 gtggaagttc gtcaccagcg catcgaccac tttgtactgg aagcccggat agataaagat
    21361 ttgggtatcg tcaaagaacg gttcgataag atcgtttttt gccgcctgtg ccgcgctttc
    21421 cagcgaacgt actgaagtgg tgccaaccgc aatcacccgg ttaccgcgcg ctttcgccgc
    21481 cagtaccgcg tctaccacat cctgcggtac ttcagcgtat tccgagtgca tgatgtgatc
    21541 ttcaatggtg tcgacgcgca ccggctggaa ggtgcccgca ccaacgtgca acgtcacaaa
    21601 cgccatctcc acgcctttgg cgcgtaattt ttccagcaaa ggctcgtcaa aatgcagacc
    21661 agcggtcggg gctgcaaccg cgcccggttt ttcgctataa acggtttgat aaagttcgcg
    21721 gtcagcgtct tcgtccggac gatcgatata cggcggcagc ggcatatggc cgatgctgtt
    21781 gaggatatcc agtaccgagc gttcatcatt aaattcgact tcaaacagtg cgccgtggcg
    21841 cgcggtcatt gttgcgttga tactttcgtc atcgcccagc aacagttctg cgccaggttt
    21901 tggcgctttc gaggcgcgaa tatgcgcaag aatgcgtttg tcgtcgagca tccgctcaac
    21961 cagcacttca atcttaccgc cgctggcttt acgcccaaac agacgcgccg ggatcacgcg
    22021 ggtattatta aaaaccagaa gatcgccggg gttgagctta tcgagtaaat cggtgaaagt
    22081 accgtgcgtc agcgcacccg tcggcccgtc cagcgacagt aaacgacagc tactgcgttc
    22141 aggcatggga tagtgggcaa tcagggattc gggcaattca aaggagaaat cggtaacgcg
    22201 catgacactg actcagacta aaataagagt cgggtagtct agtgccgggg cgctctccct
    22261 gcaaccttta cccccctttg gatagaatat aatcgatgaa ttttttagct cacctgcatt
    22321 tagcccatct cgcggaaagc tcgctttctg gcaatttact ggctgatttc gtacgcggaa
    22381 atcctgaaga aagttttccg cccgacgtcg tggctggcat tcatatgcat cgacgtattg
    22441 actgacaatc tgccggaagt ccgcgaagca cgggagtggt ttcgtagtga aacgcgccgc
    22501 gttgcgccta ttacgctgga tgtcatgtgg gatcactttc tttcccgcca ctggtcgcag
    22561 ctgtcgccgg actttccact acaggaattt gtctgttatg cccgcgagca agtgatgacg
    22621 attttgccgg actcaccgcc acgttttatc aatctgaaca attacttgtg gtcagagcag
    22681 tggctggtgc gctatcgcga tatggatttc atccagaacg tgttaaacgg catggcaagc
    22741 cgccgcccac gtctggatgc cctgcgtgac tcctggtacg atttagacgc tcattatgac
    22801 gccctcgaaa cccgcttctg gcagttttat ccgcggatga tggcgcaggc gtcacacaag
    22861 gcgttataaa acgttagttc atccataccg tagccgaaat ggcaggcaaa gccagaatcc
    22921 cgtcagtcag ttgcccatgc ccttctttgc attgccattg cacggcattg agaaacggtg
    22981 acgcgggtag caccacttca ctggcctcgc cacggttgat tgccaccagt acacgttgct
    23041 gattcagcac gcggacaaat accactacgt tatcttccgc atacagcacc tgacagccgc
    23101 cacgacgcag cgcctgactt ttcttacgca gcacaatcat tcgccggtac agcgcgaata
    23161 acgccgtatc ctgcttttcc acctgccagg ggaacggttt acggcaagac ggatcgtttt
    23221 taccatccag tcccacttca tcaccgtaat aaatgcacgg tacaccaggc caggtgaaca
    23281 gccagaccac cgccagcggc aggcgcgcaa tatcccgacc gagcagcgtt ttaaatcgcg
    23341 cagtatcgtg gctatcgagc tgattaaaca tacgtaattg ttgttgatga gaaagccctg
    23401 cgcggtaatt atccatccag gccatacagg tttgggcgtc aatttgctgc ggatcataag
    23461 agatgtcggt attggcaaga aatccccaca acgggaatgt gaagccacga tagttcatgg
    23521 cagcatcttc cacatcggcc tgtaaccatt gccgtgcatc gccaaaatgt tcgccgacaa
    23581 tatacgcttc cgactgggtt tctttcgccg cttcggtgat cccggcaacg tgctgcatat
    23641 tattgcgcgc cccacccgcc tcccccagca tatgcaccac atccagccgc cagccgtcca
    23701 tattccacgg cgctttcagc cagtggcgga caatactgtc ttccccgcga taaatttcat
    23761 tcaccagact ttccgactga taatccagct tcggcaagct ggcatagcca agccagtcga
    23821 gcgccgtgcc atcatcacta aacaagtacc agtcgcgcca gggcgattca gagttgtgac
    23881 aagctccccc cgtgccaaga ttgtgcctgt caa
//
LOCUS       DAJTYF010000054        23696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTYF010000054 DAJTYF010000000
VERSION     DAJTYF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.054"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            580..2508
                     /gene="thrS"
                     /locus_tag="OO590_002249"
     CDS             580..2508
                     /gene="thrS"
                     /locus_tag="OO590_002249"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6637604.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2512..3054
                     /gene="infC"
                     /locus_tag="OO590_002250"
     CDS             2512..3054
                     /gene="infC"
                     /locus_tag="OO590_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6637605.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3151..3348
                     /gene="rpmI"
                     /locus_tag="OO590_002251"
     CDS             3151..3348
                     /gene="rpmI"
                     /locus_tag="OO590_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6637606.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3401..3757
                     /gene="rplT"
                     /locus_tag="OO590_002252"
     CDS             3401..3757
                     /gene="rplT"
                     /locus_tag="OO590_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6637607.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3874..3998
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3880..3924
                     /gene="pheM"
                     /locus_tag="OO590_002253"
     CDS             3880..3924
                     /gene="pheM"
                     /locus_tag="OO590_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6637608.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4207..5190
                     /gene="pheS"
                     /locus_tag="OO590_002254"
     CDS             4207..5190
                     /gene="pheS"
                     /locus_tag="OO590_002254"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6637609.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5205..7592
                     /gene="pheT"
                     /locus_tag="OO590_002255"
     CDS             5205..7592
                     /gene="pheT"
                     /locus_tag="OO590_002255"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6637610.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRIAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7597..7896
                     /gene="ihfA"
                     /locus_tag="OO590_002256"
     CDS             7597..7896
                     /gene="ihfA"
                     /locus_tag="OO590_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6637611.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7997..8977
                     /gene="btuC"
                     /locus_tag="OO590_002257"
     CDS             7997..8977
                     /gene="btuC"
                     /locus_tag="OO590_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6637612.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9040..9591
                     /gene="btuE"
                     /locus_tag="OO590_002258"
     CDS             9040..9591
                     /gene="btuE"
                     /locus_tag="OO590_002258"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6637613.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9591..10340
                     /gene="btuD"
                     /locus_tag="OO590_002259"
     CDS             9591..10340
                     /gene="btuD"
                     /locus_tag="OO590_002259"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6637614.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10418..10882
                     /locus_tag="OO590_002260"
     CDS             10418..10882
                     /locus_tag="OO590_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6637615.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11129..11831
                     /gene="ydiV"
                     /locus_tag="OO590_002261"
                     /pseudo
     CDS             11129..11831
                     /gene="ydiV"
                     /locus_tag="OO590_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11895..13298
                     /locus_tag="OO590_002262"
     CDS             11895..13298
                     /locus_tag="OO590_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6637616.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPD"
     gene            complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="OO590_002263"
     CDS             complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="OO590_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6637617.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="OO590_002264"
     CDS             complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="OO590_002264"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6637618.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="OO590_002265"
     CDS             complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="OO590_002265"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6637619.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16026..18404
                     /gene="ppsA"
                     /locus_tag="OO590_002266"
     CDS             16026..18404
                     /gene="ppsA"
                     /locus_tag="OO590_002266"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6637620.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="OO590_002267"
                     /pseudo
     CDS             complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="OO590_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20164..20457)
                     /locus_tag="OO590_002268"
     CDS             complement(20164..20457)
                     /locus_tag="OO590_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6637621.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20454..21743)
                     /locus_tag="OO590_002269"
     CDS             complement(20454..21743)
                     /locus_tag="OO590_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6637622.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFIYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21799..22737)
                     /locus_tag="OO590_002270"
     CDS             complement(21799..22737)
                     /locus_tag="OO590_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6637623.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIISLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTQGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22757..23521)
                     /locus_tag="OO590_002271"
     CDS             complement(22757..23521)
                     /locus_tag="OO590_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6637624.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5847 a         5780 c         6243 g         5826 t
ORIGIN      
        1 tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc
       61 tattcgcctt atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac
      121 gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca
      181 cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg
      241 ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga
      301 tgttatcagt ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc
      361 gaatcaatgt gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc
      421 gggtggaagg tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc
      481 atttgttggc tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg
      541 atctttcgtg tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct
      601 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
      661 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
      721 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
      781 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
      841 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
      901 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
      961 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1021 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1081 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1141 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1201 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1261 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1321 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1381 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1441 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1501 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     1561 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     1621 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     1681 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     1741 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     1801 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     1861 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     1921 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     1981 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2041 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2101 gtaatgattc accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa
     2161 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2221 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2281 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2341 cgtcgcgtcc catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2401 gttcgcaccc gccgtggtaa agacctggga agtatggacg taaatgaagt gatcgagaag
     2461 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     2521 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     2581 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     2641 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     2701 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     2761 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat
     2821 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     2881 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     2941 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg
     3001 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3061 caagtagcac tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt
     3121 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3181 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3241 cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3301 tccaaaggcg atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3361 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3421 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3481 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     3541 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     3601 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     3661 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     3721 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     3781 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     3841 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     3901 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     3961 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4021 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc
     4081 ggagcgtact taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4141 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4201 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4261 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4321 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4381 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4441 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt
     4501 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     4561 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     4621 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     4681 tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     4741 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac
     4801 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     4861 agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat
     4921 ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac
     4981 gtcatgggta aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac
     5041 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5101 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5161 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5221 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca
     5281 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5341 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5401 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
     5461 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     5521 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg
     5581 gaatttctga cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg
     5641 acatccgcga atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc
     5701 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     5761 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     5821 cagtcgaagc gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     5881 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     5941 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6001 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatagcaaaa gagggcgaaa
     6061 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6121 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6181 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6241 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6301 cggtactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6361 gtgaggctgg cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6421 tcactctacg tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg
     6481 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gaatggcagg
     6541 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
     6601 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     6661 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     6721 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     6781 caggcgttga agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     6841 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     6901 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     6961 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7021 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg
     7081 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7141 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc
     7201 atcctgaact ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt
     7261 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7321 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7381 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7441 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7501 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     7561 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     7621 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     7681 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     7741 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     7801 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     7861 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     7921 atgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     7981 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8041 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8101 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8161 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8221 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8281 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8341 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8401 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8461 ctgcagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     8521 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     8581 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     8641 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     8701 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     8761 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     8821 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg
     8881 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     8941 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9001 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9061 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9121 ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9181 aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9241 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9301 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
     9361 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9421 gcccgtatgg tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt
     9481 gaaaaattcc tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg
     9541 ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
     9601 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc
     9661 gggctgggga aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
     9721 cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg
     9781 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
     9841 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
     9901 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
     9961 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa
    10021 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10081 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac
    10141 tggcgattgt gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt
    10201 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10261 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10321 tgctgatttc gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa
    10381 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10441 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10501 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    10561 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    10621 gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat
    10681 tggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    10741 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    10801 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    10861 ctggcaggcg agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca
    10921 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    10981 tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga
    11041 aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga
    11101 aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc
    11161 tgattgctat ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat
    11221 tacccatttc tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca
    11281 acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg
    11341 ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtgac
    11401 tacattatta ttagacaggg ataattacgc aggcgaacta ttaaaatatc cgtttattga
    11461 gctgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt
    11521 gtccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat
    11581 gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc
    11641 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    11701 gcatcatcgc ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt
    11761 tccatgctct ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    11821 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    11881 aacagatagg atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa
    11941 cctatacagc actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg
    12001 aactcgctaa cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg
    12061 gcggcgaaac cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt
    12121 tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc
    12181 ttgctgatgg cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc
    12241 gaatgggtga tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg
    12301 cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc
    12361 cagtgtatcg ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc
    12421 tgcgctttgg tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt
    12481 tggctgactt tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc
    12541 gtctctggtt taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg
    12601 tcggctttgc acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg
    12661 attacgggcc gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg
    12721 atcatcaagg gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc
    12781 gtctggcgca gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca
    12841 gctatcagca ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca
    12901 tgacggagca aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag
    12961 agcgcagcga ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg
    13021 cgtcaccgct acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt
    13081 atcgggggcg tttgcaacaa gacgaggtta gcgatagtga gcgtcaacaa ctgatgcaaa
    13141 gcgttaaccc agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag
    13201 aaaagggtga tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg
    13261 acagagatga tgactatgtc agtcgtccac ctgactgagg taaacggctg gaagtcagct
    13321 gctcgagtta aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga
    13381 tattcttgcc cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg
    13441 gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg
    13501 agttttctgc tatccgtata acgcataaaa attaataata attgatataa caatgattcc
    13561 tattatcaat attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg
    13621 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa
    13681 aggcgagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
    13741 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
    13801 tttccgccat aattccagca atctccgtag agccattgcg gatttgctga caaatatcct
    13861 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
    13921 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
    13981 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
    14041 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
    14101 ctgcgcggat agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta
    14161 ccggacagga gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc
    14221 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
    14281 cagtcgcggt agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca
    14341 gaccgtgatt tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc
    14401 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
    14461 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
    14521 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa
    14581 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
    14641 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
    14701 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca
    14761 tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
    14821 ttagcatgat aacaaaaaaa caaaccaata cactagttct ctagtacatt cggcgactaa
    14881 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
    14941 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
    15001 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
    15061 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
    15121 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
    15181 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
    15241 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
    15301 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
    15361 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
    15421 tatcctggca aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg
    15481 agtagaagac cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
    15541 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
    15601 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
    15661 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata
    15721 attctatgaa agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata
    15781 aaatgaacct tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    15841 accgatggtt tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc
    15901 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat
    15961 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    16021 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    16081 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt
    16141 ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt
    16201 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    16261 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    16321 ccttccagcc tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg
    16381 acgaaaacgc ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt
    16441 cctttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    16501 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    16561 agggttacga tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg
    16621 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    16681 tgtttatcac ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg
    16741 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    16801 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    16861 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    16921 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata
    16981 ttgagtgggc gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa
    17041 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    17101 tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg
    17161 acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc
    17221 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    17281 cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag
    17341 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    17401 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    17461 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact
    17521 tcgcctgcct gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta
    17581 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    17641 acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc
    17701 tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc
    17761 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    17821 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    17881 gcgactgctt cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga
    17941 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    18001 aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt
    18061 gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct
    18121 caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg
    18181 tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc
    18241 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    18301 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca
    18361 ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg
    18421 cgttcagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    18481 catcctgcgt taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg
    18541 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    18601 cccgtttacg gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt
    18661 tctgcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    18721 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    18781 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct
    18841 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    18901 ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac
    18961 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    19021 cggcagcgta accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg
    19081 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc
    19141 attcgcgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg
    19201 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    19261 ctagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    19321 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    19381 ccgttgcgtg accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc
    19441 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    19501 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    19561 tcgccgtggt cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg
    19621 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    19681 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    19741 cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca
    19801 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag
    19861 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    19921 gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca
    19981 cggcaatttt gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat
    20041 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    20101 ctttcatttt ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca
    20161 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    20221 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa
    20281 atgaatgttt cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt
    20341 acggaattca ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac
    20401 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    20461 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt
    20521 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    20581 atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    20641 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    20701 tattgcgcca gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    20761 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    20821 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    20881 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    20941 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    21001 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    21061 gtatagataa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    21121 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    21181 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    21241 cggccaagca ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    21301 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    21361 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga
    21421 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg
    21481 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    21541 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    21601 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    21661 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    21721 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    21781 atgtggttga aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta
    21841 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    21901 gatggcgaca atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat
    21961 cccctgcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    22021 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    22081 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac
    22141 gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc
    22201 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    22261 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggaaatga tcgccagcgg
    22321 gctgttgatc ttttcctgag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat
    22381 gtgaccatcg acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac
    22441 acttaaccgt gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg
    22501 gtgtttatct ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc
    22561 gtcgttttgc tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg
    22621 gtcggtattt tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag
    22681 ttcagcataa cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt
    22741 ttcttctccg taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg
    22801 cctccggcga atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa
    22861 tgccgaccag ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact
    22921 gatttaccgg ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt
    22981 taatatcgga ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat
    23041 cttcaagcgt gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat
    23101 taatcaccgg aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt
    23161 cgccggaacc ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca
    23221 gcgcttttgc ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca
    23281 ggctgtgcgg cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc
    23341 cgccaacggt cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa
    23401 tggcattgag atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt
    23461 ctggagtgac aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca
    23521 tgatttctcc tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa
    23581 aatagtgtgc gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa
    23641 tcttgcaaaa aaaatgcgat tgctaaaaca aaagtgcaat ttgcggcaat atagtt
//
LOCUS       DAJTYF010000055        23359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTYF010000055 DAJTYF010000000
VERSION     DAJTYF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.055"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(259..876)
                     /gene="tdk"
                     /locus_tag="OO590_002272"
     CDS             complement(259..876)
                     /gene="tdk"
                     /locus_tag="OO590_002272"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6637625.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQT
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1481..1894
                     /gene="hns"
                     /locus_tag="OO590_002273"
     CDS             1481..1894
                     /gene="hns"
                     /locus_tag="OO590_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6637626.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2038..2946)
                     /gene="galU"
                     /locus_tag="OO590_002274"
     CDS             complement(2038..2946)
                     /gene="galU"
                     /locus_tag="OO590_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6637627.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3148..4161)
                     /gene="rssB"
                     /locus_tag="OO590_002275"
     CDS             complement(3148..4161)
                     /gene="rssB"
                     /locus_tag="OO590_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6637628.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4253..5158)
                     /gene="rssA"
                     /locus_tag="OO590_002276"
     CDS             complement(4253..5158)
                     /gene="rssA"
                     /locus_tag="OO590_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6637629.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5271..5729
                     /locus_tag="OO590_002277"
     CDS             5271..5729
                     /locus_tag="OO590_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6637630.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5779..6621
                     /gene="purU"
                     /locus_tag="OO590_002278"
     CDS             5779..6621
                     /gene="purU"
                     /locus_tag="OO590_002278"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6637631.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6781..6865
                     /locus_tag="OO590_002279"
     tRNA            6781..6865
                     /locus_tag="OO590_002279"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6815..6817,aa:Tyr,seq:gta)
     gene            6912..7043
                     /locus_tag="OO590_002280"
     ncRNA           6912..7043
                     /ncRNA_class="other"
                     /locus_tag="OO590_002280"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7227..7904)
                     /gene="narI"
                     /locus_tag="OO590_002281"
     CDS             complement(7227..7904)
                     /gene="narI"
                     /locus_tag="OO590_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6637632.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7904..8614)
                     /gene="narJ"
                     /locus_tag="OO590_002282"
     CDS             complement(7904..8614)
                     /gene="narJ"
                     /locus_tag="OO590_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6637633.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8611..10149)
                     /gene="narH"
                     /locus_tag="OO590_002283"
     CDS             complement(8611..10149)
                     /gene="narH"
                     /locus_tag="OO590_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6637634.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10167..13889)
                     /locus_tag="OO590_002284"
     CDS             complement(10167..13889)
                     /locus_tag="OO590_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6637635.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            complement(14282..15673)
                     /gene="narK"
                     /locus_tag="OO590_002285"
     CDS             complement(14282..15673)
                     /gene="narK"
                     /locus_tag="OO590_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6637636.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16012..17808
                     /gene="narX"
                     /locus_tag="OO590_002286"
     CDS             16012..17808
                     /gene="narX"
                     /locus_tag="OO590_002286"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6637637.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17801..18451
                     /gene="narL"
                     /locus_tag="OO590_002287"
     CDS             17801..18451
                     /gene="narL"
                     /locus_tag="OO590_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6637638.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVIFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18452..19846)
                     /gene="ychO"
                     /locus_tag="OO590_002288"
     CDS             complement(18452..19846)
                     /gene="ychO"
                     /locus_tag="OO590_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6637639.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20031..20384
                     /gene="ychN"
                     /locus_tag="OO590_002289"
     CDS             20031..20384
                     /gene="ychN"
                     /locus_tag="OO590_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6637640.1"
                     /translation="MKKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20428..21123)
                     /gene="chaC"
                     /locus_tag="OO590_002290"
     CDS             complement(20428..21123)
                     /gene="chaC"
                     /locus_tag="OO590_002290"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6637641.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21281..21511)
                     /gene="chaB"
                     /locus_tag="OO590_002291"
     CDS             complement(21281..21511)
                     /gene="chaB"
                     /locus_tag="OO590_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6637642.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21781..22881
                     /gene="chaA"
                     /locus_tag="OO590_002292"
     CDS             21781..22881
                     /gene="chaA"
                     /locus_tag="OO590_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6637643.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23286..>23359
                     /locus_tag="OO590_002293"
     CDS             23286..>23359
                     /locus_tag="OO590_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6637644.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITA"
BASE COUNT         5862 a         6095 c         5620 g         5782 t
ORIGIN      
        1 gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc ttcatcatat
       61 ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc acaagtcaac
      121 gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc gagaagggta
      181 tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct gctcaccctg
      241 tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg ttaatgagcc
      301 gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt cattaccacc
      361 aattaccacc tgctcacctt cgttataagg tctgcctgtt tgatcaagac gcagcaccat
      421 gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt cggaccatgc
      481 cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta aaccataaca
      541 aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt gttgtctggt
      601 taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat gttccgcacg
      661 aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg acaaacctat
      721 acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat atacgacagt
      781 gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag acttacccgc
      841 attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct caatgatatg
      901 aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac agtatgcaca
      961 gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga gctatcatta
     1021 caactgcctc gcttgttata agcgggtaaa tgactgctgg taactattca caatctttaa
     1081 cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat actaagtcca
     1141 tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt ctcgtaaaca
     1201 caactaatac agaagactga aaggtcgtca gcctacgata atctccccat aaaatgtgac
     1261 atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat agggctatat
     1321 gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca aaataaagaa
     1381 caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc tctattatta
     1441 cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag cacttaaaat
     1501 tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg aaacgctgga
     1561 agaaatgctg gaaaaattag aagttgttgt taatgaacgt cgcgaagaag aaagcgcggc
     1621 tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa tgctgatcgc
     1681 tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat ctggcaccaa
     1741 agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg gcgaaactaa
     1801 aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg atgagcaagg
     1861 taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca cttgcttaaa
     1921 atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata agaataagct
     1981 ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat acggatgtta
     2041 cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa gggtgttatg
     2101 acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc agtcatggct
     2161 cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat caattgcgtc
     2221 ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg gccaaatatc
     2281 cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat ccgctttcgg
     2341 tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa cgcctttgca
     2401 atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct ggctatgacc
     2461 cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat cggattcata
     2521 ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca ctaccgggtg
     2581 agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt gcataatagt
     2641 cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta cacgtttttc
     2701 cagcattgcc tccagttcaa aactggtatc aaagtggttt tcaatagagt ttttagatga
     2761 gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca cgacgtattg
     2821 aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg tcgccggcaa
     2881 catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg tattaatggc
     2941 agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt ctgtatcgca
     3001 tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag atagcataat
     3061 taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt agcaggtaat
     3121 gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc gcagtcgacc
     3181 accggttccc catatttggc attgccaagc atcgcatcgc tggctcaatt gattcaaata
     3241 agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt gttcgccggt
     3301 atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt cgcgatgata
     3361 atagccaact aatagcggaa actgccccgg cagattggcc tgacgaagta aatggtttac
     3421 ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa gctgttcctg
     3481 taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat tatgtccagc
     3541 tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg caatatcaag
     3601 caccaggccg ggtttgtccg cggcaaccaa ttgacggtaa ttaacccggc aatgggaaat
     3661 cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg cagggttatc
     3721 aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg aattaaacat
     3781 gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt taactggttt
     3841 cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca tattttcagt
     3901 ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct ccagcagttt
     3961 aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag tgaaacctcc
     4021 cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc ccaatgagga
     4081 aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga gaatctgttt
     4141 tccgaccaat ggctgcgtca tgttctctcc cctgaccggc tttactcaat agtggcatgc
     4201 tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct ggtcaaatgt
     4261 tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc tgtccggccg
     4321 caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa acaggttgaa
     4381 tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc tccaacacct
     4441 ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga cgtgtcgtta
     4501 tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca ttctcgctat
     4561 tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca tcgtgctgca
     4621 ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata ggaattgggt
     4681 taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc atgagtcctg
     4741 gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac cataattcac
     4801 gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt tcgatctctg
     4861 tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt aacccaccgc
     4921 gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag gttacccaat
     4981 cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa ccaattgaac
     5041 atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata acgccaatat
     5101 gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc tttctcatta
     5161 ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc ggttaacata
     5221 gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattg gtgtctcagc
     5281 tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat gtgtctggtg
     5341 aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct tttgtgatgc
     5401 aagacgctga ttatttaata aagacctggc atccctcttg tggagcagcg gcgttacgtg
     5461 ccgaattgat gaccggattt gcgcatactg aatggttggg attgaccgta tttgaacatt
     5521 gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt accgaaggtg
     5581 gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt caatggtact
     5641 atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt ggttcaggta
     5701 aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc aaacactctc
     5761 aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac tatttgtccg
     5821 gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga gttaaatatc
     5881 gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg cacggaactg
     5941 gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt gccagaaggc
     6001 tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt cactaaagaa
     6061 gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggtctgga tgtcgaaatc
     6121 gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt tgatattccg
     6181 tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa gatggcggac
     6241 gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg ggtattaacg
     6301 ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc attcctgcct
     6361 gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa gattattggc
     6421 gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat gcaggacgtt
     6481 attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg tgacgtcgag
     6541 aaaaacgtct taagtcgtgc gctatacaaa gtactggcgc aacgcgtctt tgtttacggt
     6601 aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta atccgttacg
     6661 gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc ggcgcgtcat
     6721 ttgatatgat gcgccccgct ccccgataag ggagcaggct agtaaaaagc attaccccgt
     6781 ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat cgacttcgaa
     6841 ggttcgaatc cttcccccac caccatcact ttcaaaagcc ccggaattct caaacgaatc
     6901 tgcaatcaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa ggttcgactt
     6961 gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc gcgcagcgct
     7021 gagtaatcct tcccccacca ccatcgcttc cttgaataat caaaacctgt tactccgagc
     7081 taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct gtaggtcgga
     7141 taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac cccgccgaag
     7201 cagggtctat gtgaactaaa attcgtttag tgacgagcgc gcaccagctg gtacttacgt
     7261 gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg gaacagcagg
     7321 aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc atcgaggtgt
     7381 tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag tttcatcatc
     7441 tcgctaccgt ccatatgctg agcggagaac ggaatggtca gcaggcccag cgcgcactgg
     7501 ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg cacgcgtggg
     7561 ctgaacatac gacgtttcag caaaagcacg ccgccaatca gacacagcac gccgctggca
     7621 ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc ttcatacatc
     7681 cagtgcggcg tcaacatacc gaagaagtga ccgacaaaaa tccccagaat accgatatgg
     7741 aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga cgccgcgcgc
     7801 caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac cgccccggct
     7861 atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc tgtcctccgg
     7921 tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga tgagcagtga
     7981 ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct tcccagacag
     8041 catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc gccactttgt
     8101 cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg gcataacggc
     8161 tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata tctttcaaac
     8221 cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc ggcagatggt
     8281 ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc agcaggtcca
     8341 ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc aacagtgacg
     8401 tggcgcggcc acggtcgaac agttcgctgt actggacctg ggcatcgagc ggatccatcg
     8461 ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac agatttttcg
     8521 acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca tccggatatt
     8581 caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt tgctggtcac
     8641 atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg agccgtggca
     8701 gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc gtgccagttc
     8761 acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca gataacggta
     8821 catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg tatcaacttt
     8881 accgtcaaca gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt tcagcgcacg
     8941 cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta ccggaatacg
     9001 caagctttcg acgtctggca gaattccgtt gctgcccagc tcgcccgcgt ctgctgcaga
     9061 ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat attctggatg
     9121 cagcggcagc gccagtttcc attccatcgc cattttataa accggcgact gctgtgcagc
     9181 ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt tcggatcgtt
     9241 cggatcgagg aacacgtcca gctgacgctg gtagagatct ttctcgttct cggtgctggc
     9301 tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga tacgaccgac
     9361 acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc agaagatgca
     9421 cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacggac atccggtgat
     9481 gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat cttcttcacg
     9541 cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc agtgttcgca
     9601 caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg ccttctggat
     9661 gttgtcgaag ttcttgtctt tcgccagttt atcaaactca ccgcccaggt catcttccca
     9721 gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac gcggacgggc
     9781 aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgataat caaaatcgaa
     9841 cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcaaagattt tacccagcag
     9901 catggctcgg ttacccatgc gcggttgcag tttgccattg attttacgga tccagccgcc
     9961 tttgtatttt tcctggtttt cccagtcagt cgggaagccc tggcccggct tggtttccac
    10021 gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt tacaggtaac
    10081 tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga cttgtgaacg
    10141 aattttcatt ttacgctctc ctgtacctag tcattgcctt cgccatctaa ccagtcaatg
    10201 ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt gccgtagtag
    10261 ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt cggcgtgata
    10321 cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc aggcaggtta
    10381 acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg ctggctgaca
    10441 accgcacggg cagtcagtgc accgttgctg ttgaagactt caatccagtc gttatcggcg
    10501 atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc gcgacctaaa
    10561 gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg gtgcggcgtc
    10621 aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac ttctttcacc
    10681 gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc acgcatccac
    10741 tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat cagctcgtga
    10801 acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga ccaggtcggg
    10861 ctggagataa ttttgcgcgg ctgtgcctga atatcgcgga agcggatctt ctcgtcttct
    10921 ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc agcccaggct
    10981 ttcacggcta cctgaccgtt ggtttccggt gccagcgtca ggatcatctc tgccgcatca
    11041 attgcggtgt tcagcatcgg ctggcctttt gccggacctt ccgctttggt gtagttgagc
    11101 ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc gccgttaccg
    11161 attttctcca tcagcgggcc gatagaggta aagcgttcgt aagttgccgg ataatcgcgc
    11221 tctacgacca taatgtgcgg tgcagttttg cctgggatca ggtcgcattc gccttttttc
    11281 cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcgtgctg gataggcagc
    11341 gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa tttcttcgcg
    11401 atggctttgt agatttccca gtcgctttta gcttcccagg ccggatcgac cgccgcagac
    11461 agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc gtaccaggtc
    11521 gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa gtccagcgta
    11581 accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc ttccggcttc
    11641 acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc cagcaggtac
    11701 ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg ccagatgaac
    11761 aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg gatggaaccc
    11821 tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc cgcttcgcca
    11881 gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc catgcgttcc
    11941 gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc catcggtgac
    12001 agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt atagaaataa
    12061 gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc cagcggctgc
    12121 cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc gcccccgctc
    12181 tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt catatcgagg
    12241 tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg cgttttatca
    12301 gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac gccggtaatc
    12361 tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc acagttaacg
    12421 tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac ggtggtcacc
    12481 agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg cagcagcacg
    12541 ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta cgggaagcct
    12601 acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt ttcttcgcca
    12661 gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg gaagccaata
    12721 gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt ccattccgga
    12781 ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg acctgcagcg
    12841 tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta gcgacgcaca
    12901 tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat tacgtggccc
    12961 atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag atcgcacagt
    13021 ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt gtaacgcact
    13081 tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc ccaggcgatg
    13141 atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc gccccaggtt
    13201 tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca agtaccgcca
    13261 atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc cggaattggc
    13321 gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt agatgcggcg
    13381 atcagttcgt tcacctcctg ccaagaagaa cgaacaaatc caccgcgtcc acgcgcctgc
    13441 ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc aaccggatcg
    13501 ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat catcgggtat
    13561 ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg gcagccgcga
    13621 ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg ggtttcccag
    13681 gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca gtttaccccg
    13741 tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc ctcccagtca
    13801 cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt ctgtttgaag
    13861 tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc tgtcggccct
    13921 ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac agcgtgggaa
    13981 ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag ataccccttt
    14041 aggataatta acagttacag cataacatgc tgtaaattaa gacgttttat acctgtagtt
    14101 atttaaacta ttaagatttt tatgcaaatg ggtattaagg agtattcccc atgggtaaca
    14161 tattaatggc tcttaatgaa atccggcgaa ataagccata taacactaat atgttgtaac
    14221 taaatgacgc ataaaaaaag cgcggcatca tgccgcgcaa aggataatca aatattaaga
    14281 tttatttttt agaatgccga ccatataccg cccaagtaat cactacgcag gcgatataga
    14341 aaatcaaaaa tactttcatt gcacctactg gcgaacccgt taatgccagc gagctaccaa
    14401 acgctttcgg gataaagaag ccaccaatcg cgccaatcgc agagatgaaa cccagcgccg
    14461 ctgccgtgcc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct tctgctttca
    14521 cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg gaaccactac
    14581 ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg aagcttccgc
    14641 cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc atcagaataa
    14701 agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca cctgcagaac
    14761 gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga acatccggga
    14821 actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gatgaaggag ccgaaggttg
    14881 ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc aactgctcct
    14941 tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca atggtgaaga
    15001 tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca gtcccatccg
    15061 gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc accagcggag
    15121 caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga cccagcgcgc
    15181 caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat gcgaagttcg
    15241 cgccagcaaa accgcatagc agagagatga tgatgaagac gctataaggc gtggaggtat
    15301 cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg ctgaacgccg
    15361 tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt aataacgcgc
    15421 cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa ttaaaaccga
    15481 ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac gccagcagca
    15541 gacagggaac ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt tgttgccaga
    15601 acgcaggatc ttccggtcgc caatctgtta tgacagctcc agtagccctt tcggaggcag
    15661 atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt agtgtctgga
    15721 gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca ttgatattta
    15781 tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc acggtgctgt
    15841 agaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag gagtaactct
    15901 ttccggctat gggtatactt cagccaatag ccgagaatac tgccattcca gaatatatcg
    15961 tcacattcat taaggttatt gctcatttaa agcctgaagg aaggggttta catgcttaaa
    16021 cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt gctttctact
    16081 gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt tcagggcagc
    16141 gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct gttggcggca
    16201 gtgccattaa gcgagaaaga caagccctta attaaagaga tggaacaaac ggcatttagc
    16261 gccgagttga ctcgagcagc agaacgagac ggacaactgg cgcaattaca gggtttacaa
    16321 gattactggc gtaatgaact gatccctgcg ctgatgcgtg cacaaaaccg agaaacggtg
    16381 tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg ttttgaccgc
    16441 accacggaaa tgcgtattga gacagtggta ctggtccatc gggtaatggc ggtatttatg
    16501 gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca accgtggcgg
    16561 caactgctgg caatggcgag tgccgtcagc catcgcgatt ttacccaacg cgcaaacatc
    16621 agcgggcgca acgaaatggc gatgcttgga actgcgttga acaatatgtc tgcagaactg
    16681 gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg gctggagcat
    16741 aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc ccgcgccccg
    16801 ctgtgtgaac gcctgtcacc ggtactcaac ggcttacaga atttaaccct gctacgtgat
    16861 atcgaactgc gggtgtatga cactgatgat gaagagaatc atcaggagtt tacctgccag
    16921 ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt attacccgtt
    16981 ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca gtacggtatt
    17041 ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca actggtggat
    17101 accctggttg aacaacttac cgccacgctg gcgctggatc gccatcagga acgtcagcaa
    17161 cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga ttctattgcc
    17221 caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg cgatgcgctg
    17281 ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc atcctgggcg
    17341 cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg attacgtccg
    17401 gcgctggaag cgagttgcga agagtacagc gccaaatttg gcttcccggt gaagctggat
    17461 tatcaattgc cgcctcgtct ggtgccttca catcaggcaa tccacttgtt gcaaattgcc
    17521 cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaggtcgt ggtgacggtg
    17581 gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg cgtgcctgaa
    17641 aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc gcaaagttta
    17701 cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt ggtcaccttt
    17761 attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc aggaaccggc
    17821 tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac agcttatcag
    17881 tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg gtattgaact
    17941 ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg gcatgaacgg
    18001 tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg tgatattcag
    18061 cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg atggctatct
    18121 gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag ctgctggcga
    18181 aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg ctaaccgtgc
    18241 cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc tcaagctgat
    18301 tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg aaagcacagt
    18361 aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg tggaagcagc
    18421 ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt tcgtcgtttg
    18481 acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag acacgctgcc
    18541 cctggttatc ttccaccacc acagacaatc gccagtgatt gcttgcccct tccccgttct
    18601 gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg cccggcgtca
    18661 aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa cgactgcgca
    18721 tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt gtcgccagaa
    18781 acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt cgctgcggat
    18841 tgtcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca gaaagttgtt
    18901 ttttgagcgg tacaccaaag cggtaattaa gattcagccc gaggttattt tgattttcgc
    18961 cactttcccc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg gtgtaattta
    19021 atcccagact caacgcgacg ggattgtgat aacccgtacc aaagttaaac aaatcaacac
    19081 gatcaccaaa atactgttct aggctgacac tggtattgag gtgttgatag aacggcatgc
    19141 gcatccgagc tgtcaggtcg tacccgcgcg ccatccgttg ttcctgcgtg gctgtctgtt
    19201 catgccatgc agcaaacggc tgataaaagt ttgccgataa tcgcaaatat tcgccccacg
    19261 cttcggcacc aaagcccgct cgctgaagat tttcgtcctg caagttgtca taaaaagtgt
    19321 tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca ttgctcacca
    19381 acccattatc ctgctgagta agaccaagct ggctccaggt gagataacga tcattatctt
    19441 gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta tccactttga
    19501 cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga ttaacctgtt
    19561 gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg ccagtatcca
    19621 gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca aagtgttttt
    19681 ccccatcatg attttcggga gccatgccta aatccggcag accatcattg ttattatcaa
    19741 agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg cctgctacaa
    19801 gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg tcggtaactt
    19861 ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac ttttgtcgta
    19921 ttttctcgcg ctttcaggaa aatccttaaa aatgtgaccg attggtcaga cgctgcagaa
    19981 gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata atgaaaaaaa
    20041 tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac agcttgcggc
    20101 tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc ctgatgtctg
    20161 atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac attcagcaaa
    20221 tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc tgtaccgacg
    20281 ggcgcgggat tagcacactt cctctgattg atggggtgga aatcggtact cttgtggaac
    20341 tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgttta agggggcgtc
    20401 tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccttaaca caccatccgg
    20461 ataattctcc gccagtagtt tttttaccga taccagcaaa tcattcagcc catcgtcctg
    20521 cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcat tggttcccag
    20581 cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt cagattcata
    20641 ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc catcatcaag
    20701 atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct tccacaacag
    20761 ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg tggtgcgtcc
    20821 gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc cggcggtcag
    20881 gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcatgatt cggtaaactc
    20941 cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc cctcatcagg
    21001 tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc ataagagtga
    21061 ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat cacgcgttat
    21121 caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt cttccagaca
    21181 tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg accacttgca
    21241 aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt ttatgccatt
    21301 tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact ttatgcgcgg
    21361 tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga tcccatgcgc
    21421 tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc tttacgcttt
    21481 ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa gttgaacatt
    21541 aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt cagactgttc
    21601 ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg ccccaatgag
    21661 taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag acactttctg
    21721 ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg gataaccata
    21781 atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct tattttcccg
    21841 gttctggcgc tggtagtgct gttcctgtgg ggaagtagcc agacactacc agtggtcatt
    21901 gccatcaatc ttcttgcgct tattggtttt ttaagtagcg cctttagtgt tgtccgtcat
    21961 gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct tagcctttca
    22021 gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga cgccgcgcca
    22081 acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg gctggttggc
    22141 ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct gtttggtatc
    22201 aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt tccaatggct
    22261 ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat ttctgcggca
    22321 atgtatggcg tatttttgct gatccaggcc aaaacgcatc aaagtttgtt tgtctacgag
    22381 cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc ccatagcagc
    22441 ctgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt caccaaaatg
    22501 aacgccagct cgctggagac attgctcgac agcatgaatg cccctgttgc ctttactggc
    22561 ttcctggtgg cactgttgat tctgtcgccg gaaggtttag gtgcgttaaa agcagtgttg
    22621 aacaaccagg ttcagcgcgc gatgaatctg ttctttggtt cagtgttagc aacaatttcg
    22681 cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt gcagtttgca
    22741 cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca tatctccttc
    22801 tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt tgccgcctat
    22861 ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc gggagagata
    22921 agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt aatcagaatt
    22981 gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa gccggaatgt
    23041 actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt agtatccaca
    23101 ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
    23161 gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac aaaacggggg
    23221 ctaatcttga aaccagacac ttcaagaata gccgcaaaat gttgtcatta caacaaggcg
    23281 gctatatgac gctcgcgcag tttgccatga ctttctggca cgacctggca gcaccgatcc
    23341 tggcggggat tattaccgc
//
LOCUS       DAJTYF010000056        23204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTYF010000056 DAJTYF010000000
VERSION     DAJTYF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.056"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..1103)
                     /locus_tag="OO590_002294"
     CDS             complement(<1..1103)
                     /locus_tag="OO590_002294"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6637645.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALHRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNA"
     gene            complement(1175..1564)
                     /locus_tag="OO590_002295"
     CDS             complement(1175..1564)
                     /locus_tag="OO590_002295"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6637646.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1578..3871)
                     /gene="tdcE"
                     /locus_tag="OO590_002296"
                     /pseudo
     CDS             complement(1578..3871)
                     /gene="tdcE"
                     /locus_tag="OO590_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            complement(3905..5113)
                     /gene="tdcD"
                     /locus_tag="OO590_002297"
     CDS             complement(3905..5113)
                     /gene="tdcD"
                     /locus_tag="OO590_002297"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6637647.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5139..6470)
                     /gene="tdcC"
                     /locus_tag="OO590_002298"
     CDS             complement(5139..6470)
                     /gene="tdcC"
                     /locus_tag="OO590_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6637648.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMAGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6492..7481)
                     /gene="tdcB"
                     /locus_tag="OO590_002299"
     CDS             complement(6492..7481)
                     /gene="tdcB"
                     /locus_tag="OO590_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6637649.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAILGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7580..8518)
                     /gene="tdcA"
                     /locus_tag="OO590_002300"
     CDS             complement(7580..8518)
                     /gene="tdcA"
                     /locus_tag="OO590_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6637650.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            8708..9052
                     /gene="tdcR"
                     /locus_tag="OO590_002301"
     CDS             8708..9052
                     /gene="tdcR"
                     /locus_tag="OO590_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6637651.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            9308..9847
                     /locus_tag="OO590_002302"
     CDS             9308..9847
                     /locus_tag="OO590_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637652.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            9869..11045
                     /locus_tag="OO590_002303"
                     /pseudo
     CDS             9869..11045
                     /locus_tag="OO590_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
     gene            complement(11655..12031)
                     /gene="rnpB"
                     /locus_tag="OO590_002304"
     ncRNA           complement(11655..12031)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO590_002304"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(12064..13215)
                     /gene="garK"
                     /locus_tag="OO590_002305"
     CDS             complement(12064..13215)
                     /gene="garK"
                     /locus_tag="OO590_002305"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6637653.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHTWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(13312..14202)
                     /gene="garR"
                     /locus_tag="OO590_002306"
     CDS             complement(13312..14202)
                     /gene="garR"
                     /locus_tag="OO590_002306"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6637654.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(14232..15002)
                     /gene="garL"
                     /locus_tag="OO590_002307"
     CDS             complement(14232..15002)
                     /gene="garL"
                     /locus_tag="OO590_002307"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR6637655.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(15018..16352)
                     /gene="garP"
                     /locus_tag="OO590_002308"
     CDS             complement(15018..16352)
                     /gene="garP"
                     /locus_tag="OO590_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6637656.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            16727..18298
                     /gene="garD"
                     /locus_tag="OO590_002309"
     CDS             16727..18298
                     /gene="garD"
                     /locus_tag="OO590_002309"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6637657.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(18350..19174)
                     /gene="agaR"
                     /locus_tag="OO590_002310"
     CDS             complement(18350..19174)
                     /gene="agaR"
                     /locus_tag="OO590_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR6637658.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            19423..20223
                     /locus_tag="OO590_002311"
                     /pseudo
     CDS             19423..20223
                     /locus_tag="OO590_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
     gene            20223..20687
                     /gene="agaV"
                     /locus_tag="OO590_002312"
     CDS             20223..20687
                     /gene="agaV"
                     /locus_tag="OO590_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6637659.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            20698..21477
                     /locus_tag="OO590_002313"
     CDS             20698..21477
                     /locus_tag="OO590_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR6637660.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            21467..22276
                     /locus_tag="OO590_002314"
     CDS             21467..22276
                     /locus_tag="OO590_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR6637661.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            22363..22797
                     /locus_tag="OO590_002315"
     CDS             22363..22797
                     /locus_tag="OO590_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6637662.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            22794..>23204
                     /locus_tag="OO590_002316"
     CDS             22794..>23204
                     /locus_tag="OO590_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6637663.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAH"
BASE COUNT         6063 a         5909 c         5471 g         5761 t
ORIGIN      
        1 gcattgcata cctgcgccgg actgccgccc agtagttcag ttaaccctgc cgctgccatt
       61 gaacaggcca cgccaatctc cccctgacag ccgacctccg cgccagagat ggaggcgttc
      121 attttataca gcgcgccaat agcccccgct gccagaaaat agcgggcaat tgaccgctcg
      181 tttaccggac gacggaactt atcgtaataa gccagcactg ccggaataat gccgcacgca
      241 ccgttagtcg gtgccgttac cacgcgcccg ccagctgcgt tttcttcact aaccgccagc
      301 gcgtacatgt tgatccagtc gatgacattc atcggatcgt tagagatgtt atcgctagaa
      361 accagctgac gatgcagcgc tacggcacga cgcggcacat tgagcggacc aggcagcacg
      421 ccttcagtgt tcatcccgcg ttcaataccg tcatgcatca cttgccagat acgggcaaac
      481 cctgcgtcaa tttccgcttt gctgcgcagc gccagctcgt tgtgcatcat caggccagat
      541 atagacaggc cgttgtaatc acacattttc agcagttcac ctgctgagtg gaaatcgtaa
      601 ggtacggacg tttcgacatc gtgcgacagg ccgaagtgtt cttcttcgac aataaacccg
      661 ccgccgacag agtaataggt tttacttaat agcgcttcct gtcccttcca ggcagtgatc
      721 cgcattccgt tctcatggcg aggcaacatt tcgggatgga agataatgtt ctttgctaca
      781 ggaaaatcaa caatatgcgc accagatgcc actggcagcc gcccgctgcg cgttactaac
      841 tctataaatg cagggatctc atcaatgaca acatcctgcg gactgtttcc tgccagtccc
      901 atgataatgg cgacatccgt ggcatggcct ttgcccgtca gtgacaacga cccgtacaga
      961 tcgaccacaa tatggctcgt cgcggttaat aagccgctac tttccagccg atcaataaaa
     1021 ctttttccgg cattcattgg ccccacggta tgcgaactgg agggaccaat cccaattttg
     1081 aaaatatcga atgcactaat catatccaca ccctcggatt gccgttcagt gaagtggagc
     1141 ggaacgacct tgcgaccgtc ccgctcacga ggctttacgc actacgtact gcgatagctt
     1201 caatttccag cttcacatct ttcggcaaac gcgcaacctg cacacagctc cgggtcgggt
     1261 aggtcgcctg atgctcatcg aagaactgct tatagacttc attgatggtg gcaaaatcat
     1321 tcagatcggt gatgaacacg gtcatcttga tgatatcgcc cacgctcagc ccggcagcaa
     1381 ccacgatcgc tttgacgttt tcgaggctta aacgcgcctg atcttgcaca tcagccggga
     1441 tctcaccggt ctgcgggcaa accggaattt gcccggaggt gaagaccatg ctgcctaaat
     1501 caacgccctg aacataaggg ccgattgcgc ctagggcacg ttgcgtttcg ataatctttt
     1561 tcatacatcc tccggcgtca gagcgcctgg gtaaaagtac gtgaaataac atcctgttgc
     1621 tgttcacggg tcagtgcgtt gaagcgcacg gcgtagccag agacacggat tgtcaggtta
     1681 ggatattttt ccgggtgctc gatggcatcc agcagcattt cccgattcat tacgttgacg
     1741 ttgaggtgtt gaccgccttc gacatccgct tcgtggtgga aatacccatc cagcaggccg
     1801 acaaggttgg ttttacgtac tggatcttct ttgcccagcg ccgcaggaac gattgagaag
     1861 gtgtacgaga tcccatcttt ggcgtaggtg aacggcagtt tcgccaccga cgtcaatgag
     1921 gccacggcac ctttgcggtc acgaccatgc atcgggttag cgcccggcgc gaacggtgtt
     1981 ccggcgcgac gaccgtccgg cgtattaccg gttttctggc cgtacaccac gttagaagtg
     2041 atagtcagaa tcgactgggt agggacggcg ttgcgatagg ttggcagcgc tttaattttc
     2101 ttcataaagc gttcaaccag gtcgcaggca atgctgtcta cgcgctcgtc gttgttgccg
     2161 tactgcggat attcaccgtc gatttcaaag tccaccgcca ggccgttttc gtcacggatt
     2221 ggtttcacgc gggcatattt gatggcagac agggagtccg tcgccaccga caggcccgcg
     2281 atgccgcatg ccatagtgcg atagacatca cgatcgtgca gcgccatcag cgaagcttcg
     2341 tagctgtact tgtcgtgcat gtagtgaatg atattcagcg cgctgatgta ctgcaccgcc
     2401 agccagtcca tgaagtgatc gaggctgtcc atcactttgt cgtaatccag cacgtcgtcc
     2461 atcagcggtg ctgttttcgg cccgacctga atcttcagct tctcgtccac cccgccgtta
     2521 attgcgtaga gcagcgtttt cgccaggtta gcgcgtgcac caaagaactg catttgctta
     2581 ccaatcacca ttgggctgac gcagcaggca atcgcgtaat cgtcgctgtt gaagtcagta
     2641 cgcatcagat cgtcattttc atactgcaag gaagaggtga cgatcgacac ctgcgcggca
     2701 tattttttga aggcaatcgg taattcttcc gaccaaagaa tggtcaggtt aggttccggt
     2761 gccggcccca tagtgtgcag gtgtgcaaat agcggaagga gtttttggtc accagcgtac
     2821 gaccgtccag ccccatcccg ccgatcactt ccgtcgccca gattgggtcg ccggagaaca
     2881 gcgaatcaaa ttccggtgta cgcaggaagc gtaccatacg gatcttcatg atgaagtgat
     2941 cgatcagttc ctgtgcctgc tgctcattga ggacgccagc tttaaagtcg cgctcaatgt
     3001 agatatccag gaacgatgcc gtgcgcccca gcgacatcgc gccgccattt tgcgatttca
     3061 ctgccgccag ataagcgaag tagagccact gcaccgcttc ctgtgcattc tgcgccgggc
     3121 gagagatatc aaagccatat ttcgccgcca tttcctgaat ctgcaacagc gcatgacgat
     3181 gctctgccag ctcctcacgc agacggatgg tggcttccag atcctcgcct ttttccagac
     3241 gagactggag atcggcaaat tgcagttcgc gttcacgcac cagataactg atgccataca
     3301 gcgctacgcg gcgatagtca ccgataatgc gcccacgacc atagccatct ggtaaaccgg
     3361 tcagcacgcc agatttacgg cagcgcagca tatccggtga gtaaacatca aatacgccct
     3421 ggttatgggt tttacgcaga tcggtaaaca gatattcaaa ttcactgtcc atttcacggc
     3481 cataggcgtg gaatgaactt ttaatcatat taatgccacc gaacgggtgt agcgcacgtt
     3541 tcaacggcgc atccgtttgc aggccaacaa ttttttccag cggctggtta atatatcccg
     3601 catcatgagc ggtaattgtg gtggcaatat tggtatcgaa atcaaccggc gcgtgggttg
     3661 cgttttcgat acggatgcct tccattactt tttctcacaa ttccgtggtg gcaggcgtcg
     3721 cttcggcgag aaaagattca tcgccttcat acggtgtata gttatgttga ataaaatcgc
     3781 ggacattaat ttcgcttttc cagtccgtac ctttaaagcc aagccatgcg tcagcgtaca
     3841 gcttatcgct ggtatcaata tctaccttca tgaaaaataa tctctctaca atacttcaac
     3901 taaattatgc aaattctgcg ggcgcgttaa ctttacctaa atgaatggca tccaaagcaa
     3961 tcattttttc ttcgttagtc ggaataacga cgcaaatgac atgcgcattt tcactggaaa
     4021 caattcgctc gccaaaagag ttagatcgat tattcatttc tgtatcaatc tctacgccta
     4081 atacagccaa atgttccatg accagacgac gaattaaact tgaattctct cctattccgc
     4141 cggtgaatat aattccatcc agccgacgta atgaagctgc atgtccggca atatgacggg
     4201 caattcggtg aacaaaggtt ttaattgcca gttgcgcgcg ttcgtgacct tcatgccagg
     4261 ctttttccag aacacgtaaa tccgaagaaa gaccggaaat acctaataat cccgactctt
     4321 tattcactac gcgttccagg tcacccaggc tctggttggt ttggctggcg acccaggaca
     4381 tcgcaccaaa gtcgacatcg ccactgcggg tacccatcat caagccttcc agcggcgtca
     4441 ttcccattga ggtgtcaaca ctctgaccgt tgcgaactgc gcagattgac gcgccattgc
     4501 caagatgcgc cacaaccagg ccggagtcat cttccgccag attcagcagc gaatgtgcgc
     4561 gctgggaaac atagcggtgc gacgtgccgt ggaaaccata acggcgtaca cctaactctt
     4621 cataatattt ccacggcagg ccgtataaat aagcttccgg agccatcgtc tggtggaaac
     4681 tggtatcaaa tacggccacc tgagttacgc ccggaaataa ttgctgcgcc gattcaatac
     4741 cacttaaatt tgcgtaatta tgcaggggtg ccagtggaga aacgcgacgg atattatcaa
     4801 tgacttcatc ggtaataatg gcggactcgg taaaaatact gccgccgtga gcgatgcggt
     4861 ggccaattaa ggccacgctg tcatttaaat tccgtttttc cagttcaaat gcaattgcct
     4921 tcaatgcacc ttcgtagctg tggagagcca gcggtgctgg ctctccccca tttacggata
     4981 agaatgcatt ttccgagtta ataccgtcgg caatacctga cattaatact tcacagtcgc
     5041 tggcatcgag tacggaaaac ttaatcgaag acgaaccaca gttaataacc aaaacaaccg
     5101 gaaattcatt catctctttt ctcatcctga gttacggatt aaaacagttt gtatacgatg
     5161 ttcaggatgg tcagcagacc aatcacggta acaaacacgt tatccagacg accacggtat
     5221 ttcgccagag acggcgcttt acggatagca tacatcggca acaggcacag cagggatgcg
     5281 ataatcggtg cgcccatggc ttcaatcagg tcgaggatgt tcgggttggc gtaggcaaca
     5341 acccaggtgg agcccatgat gaagatcatg ctgagagtat tcagtttacc cagcgacact
     5401 ttggttttgt cacctttata accgaacttc agaatcagac cattcaagcc ttccagcgtc
     5461 cccagatagt gaccgaagaa agatttgaag atagccacga gtgcgatgat ggaagccgca
     5521 tattccagtg taatcgcgaa cgttgttttc gtaccggcca tggacgcaaa gtggttagcc
     5581 agataagaaa gcactggaat attctgcact ttggcttccg ccatgttggc cggagacaga
     5641 gtaaacaggc agctaaaggc aaagaacatc accactgcaa ccatcagcat gctggcacga
     5701 gaaatgattt gggaacattt acgttcggtg aagtcgcgac cgaagtcttt ctcatactct
     5761 tcacgtttag aaaccacgaa ggaagagacg attggcgaga agttaaagga gaaaaccatg
     5821 atggaaatcc ccagccagac agtgatcagg ataccgtcat gaccggttaa cgacagcgaa
     5881 ccgaggtcaa cctggtcgat aactgcagag ttccagtaag ggatcagcga cagagaaatc
     5941 agcaccaggc tggcgataaa cggccatacc aggtagctca tcactttaac catcagatcc
     6001 ttaccaaacc agatgacgaa agccatcagc agcaacagga acagcgccac aaagccgcga
     6061 ttcagcggtg caaatccgag ctggttttcc cagaacgtca taaaggtatt ggtaatagta
     6121 acgccataaa tccacagcag tgggcaaatc gcgaagaagt acaggaacgt gataaccacg
     6181 ccgccagttt taccaaaatg ctcttccacc gtttccgtaa tgttgccgga agggttagag
     6241 ccagaaagac acagacgcgc cagcgcccgg tggcaataaa atgcgatggg gtatgccaat
     6301 accaacatca gaagaatcgg gatcagtccg ccaaaacctg cgcggatagg gaagaacagc
     6361 accccggcgc cgattgccgt accaaacaag cctaacgtcc atgtggtatc tgatttacgc
     6421 caggacgatt gttttgtctg gctggataca atgctatctg aagtactcat atcctatcct
     6481 caacgaatta attaagcgtc aacaaaaccg gtgatttgag agacgcgaga aagatcgata
     6541 ttgccgccgg aaataatact gacggttttt ctgttttgaa tatattggtc taatttaccg
     6601 cttaataatg cagcacatgc cagagcgcct gcgccttcgg tgacgacttt attgcgctga
     6661 attaaggcaa tcatactgtt tctgatttcg tcttcgctga ccagcacgat gtcatcgact
     6721 aattcacgaa cgatttcgta agttaaatta cccgggcggg agacatcaca accatccgcc
     6781 agggtgccgg tagttcggtg cgtggttatt tctccggagt ggaaagaagc cgccatgccg
     6841 tgaacgtttt ctgactgtac gccaataaca cgaatggtcg ggttaataga tttaattgcc
     6901 accgcaatac cagcaattaa accaccacca ccaattggca caatcacgtt atcgacatca
     6961 tagagatctt ccataatttc cagaccaatc gttccctggc cagcaatcac tttcggatca
     7021 tcgtaaggtg ggataaaaat acgaccttcc atttcgacaa tttcgctcac tttagcgata
     7081 gtgtcgttga agttatcacc atgcagaacg acttctgcgg agtagtcgca cgttgccgct
     7141 actttggatt ttggcgcacc ttttggcatc accactttac cgtcgatacc cagtatcgcg
     7201 caggagagcg aaaccccttg cgcatggttg cccgcagaac aggccaccac gcctttgcgt
     7261 ttttccgcat cggtcagtga acttaattta ttaaatgcgc cacgaatttt aaatgaaccc
     7321 gtacgctgca tattttcaaa cttcaggaat atttcacctt tgcaacgttc actaaaatag
     7381 ttggagcgag gcatgcctgt tttataaatt cgcccagcca gtcgttgttt cgcttcaata
     7441 atatcatcaa tagcaaccgg cagatcgtat gtaatatgca ttataaaacc tcttcgcctg
     7501 aattaaatat gtaggtaacc gtaaccgaca cctgcaaaac agacaggtgg attatttatg
     7561 tttaaataaa aacaaataac taaccaactt ctattaattg ccttcgtcta cacccattat
     7621 aagatgaata ctctttggct aattccacca aaaccgatgc tgctttttta atacgataat
     7681 ttttcgacca taccgcggca tattgtgcca caggtaatgt ttcttcaacc ggaatagtaa
     7741 taaattgatt agaaccaaaa ggtgacgtca tatcacaagg aattacagtt aagaaatcag
     7801 cattgagaac aagattataa attgtcacga ctgagtcggt tttaacgatg ttttcaatac
     7861 tgatgccatt tctttgtaac gtagtaagca gttcgctgta gtaccccata ttagtttgtg
     7921 gcaacaccca ctgttcgttc ttcaacgact ccagcgtggt ggtgccggtg catgttcggg
     7981 acttactggc taccagcaca aactcggact cgaacagcgg ctcaacatgt aaatcctgaa
     8041 gcttcatttc tgcacttaac gtaccaatcg caaaatccag tcgaccgtcg cggattgccg
     8101 gtaagaacga agacagttgc gcttcataca tagaaacctg cgctttcggg aacacctctt
     8161 tgaacttgtt gatcatccct gacataaaag taaaaccaat caatgaagga aaaccaaatg
     8221 agacttccac caccgcctca gaagacatac cgcttatctc attaaccata tttttcattt
     8281 cacgggtaat ggattcggaa cgggagagta acaattgacc ggcaggtgtt aatgttacac
     8341 cggtattttt ccgcaccact aattccacac caaaataatc ttcaatatcg ttaatgattt
     8401 tactgacggc cggttgagtt aaccctaatt cttttgcagc cgagccgata gagccacttc
     8461 taatgacttc ctgaaagact accaggtgct gcgttttcgg aagaagaata gtgctcataa
     8521 cgacctacgt taattacctc attgacggca tgaagtgtat caaaatgaaa taaacaggat
     8581 atgtgcgacc actcacaaat taactttcat tactttccaa ggtatcgtta ttacaattaa
     8641 atacctttaa atatcaacaa gttaaagcat aaaaatcagc ataaaccctg atttctatca
     8701 aaaaaaaatg acggggataa ccatatttta tggcgataac atcattcgtt atgtggttaa
     8761 tacaaaaaag gggctgagac catatttcaa gcaattaccg gataattacc aggcaaaatt
     8821 tgagttaaat ttaatgagca agttctcaaa ttttattata aataaaccat tttcagcgat
     8881 aaatactgcg gcacgtcaca ttttttcacg ctatttgttg gagaacaaac atttatttta
     8941 tcaatatttt aaaatttcga atacaggtat tgatcatctc gaacaattga ttaacgtcaa
     9001 ctttttctct tctgacagga cgtcattttg tgaatgcaat cgttttccat aaattcttct
     9061 cccctcatag gcgacgaata gcattttgtg ttgaggatca caaaacgaat aattgctggt
     9121 cgccgcgata aggtcagacg aagtcaacaa gggggatttt cactgtgctt tacatcagcg
     9181 taggacacag aatgttgctt cgtttactgt aacgccgggt aaatgagcgt tttttgatag
     9241 tgcgtaagtg tcgtgcgagg aaaataagca tctattattg tttgtcatga cgacaaggca
     9301 ggcatcaatg aaaggcttcc caattgcaca tatttttcac ccttcaatcc cgccaatgca
     9361 cgcagtggtt aacaatcaca atagaaatat tgattattgg acggtaaaaa gaaagtttgc
     9421 agaaattgtc tccaccaatg acgttaataa aatttacagt ataagtaatg aactgcggag
     9481 agtattatct gcaattactg cattgaattt ctatcaaggc gatgttcctt ctgtcatgat
     9541 ccgaatccaa ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga
     9601 tgattatatc atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac
     9661 ttgctcagcc gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg
     9721 cgcaaagttc acccaaaaag aagccatctt aacaccccct aggcacttta ataaaacctg
     9781 cattacttca gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt
     9841 ttattgatta tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat
     9901 tagtagtgat ttagtccgtc gaaaaatgaa tgaccttcct gaaagcccca taggaaataa
     9961 cctcgaagca ctagcacccg gtatagaaaa actaaaacag acctctattc aaatggtcac
    10021 tttacttaac gcgttacaac caagtggaaa atgcattatc actggtgatt ttcaaaaaga
    10081 attagcgtac ttacaaaatg taattcttta taatgactcg tctcttcgtc tggatttttt
    10141 aggttataac gccaaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga
    10201 accgttaaaa aaccaggaaa tatctacagg caatatcaat gttaattgcc cattaaaaga
    10261 tatttacaat gaaatcagga ggttgaacgt aatctttagt tgtgggactg gagatatagt
    10321 tgatctatcc tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa
    10381 acatatggct aatactattc taaatcattt taaattggat tcaacaaatt ttactaatgc
    10441 caacatgttt caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta
    10501 tttactaaaa ataacgcctg ttttaataag cattagcgat atgtattctg aagaaaaaat
    10561 caagtttgtc gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat
    10621 tatgagattt gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa
    10681 tgaattaaca aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat
    10741 taaattacca gagatgaaag aattctcatc aaaaaaatat atgattacat cgatacaatc
    10801 attgaagatt atgaaaatat caaagaaaat gcttatctgg tcgtccccca aattaattat
    10861 actatggatt taaacataga agactctagc tcagaagagt taataccctc gagaaagacg
    10921 aaaattctcc ggacaatggc tttgaggtcg gggaatataa cacatatgaa gcatataact
    10981 cagagaatca atattttacc agagaggact atacgtataa ttacgacctt ttaaatgcaa
    11041 tatagactta ataacttctt acaaagtcta ttattaatac cgtagatatt catcttaccc
    11101 tgtgcaccaa aagcaaaaac ttatcgacgc gggtttgaaa ctgatggcaa ccgcaaaaat
    11161 gcctgatgcg ctacgcttat caggcctacg ccatctctgc aatatattga atttgcgtgc
    11221 ttttgtaggc aggataaggc gttcacgcag catccggcat gaataaagcg cactcaacaa
    11281 tctgaaaccc gccggaacgg tttattacgt acatcaggtg aaactgaccg ataagccgct
    11341 ttcttttggg tatagtgtcg tggacagtca ttcatctttc tgcccctcca aaagcaaaaa
    11401 cccgccgaag cgggttttta cgtatatcag gtgaaactga ccgataagcc gctttctttt
    11461 gggtatagcg tcgtggacag tcattcatct ttctgcccct ccaaaagcaa aaacccgccg
    11521 aagcgggttt ttacgtacat caggtgaaac tgaccaataa gccgctttct tttgggtata
    11581 gcgtcgtgga cagtcattca tctttctgcc cctccaaaag caaaaacccg ccgaagcggg
    11641 tttttacgta aaacaggtga aactgaccga taagccgggt tctgtcgtgg acagtcattc
    11701 atctaggcca gcaatcgctc actggctcaa gcagcctacc cgggttcagt acgggccgta
    11761 ccttatgaac ccctatttgg ccttgctccg ggtggagttt accgtgccac ggactgttac
    11821 cagccgcgcg gtgcgctctt accgcaccct ttcaccctta cctgatcccg cttgcacggg
    11881 ccatcggcgg tttgctctct gttgcactgg tcgtgggttt cccccccagg cgttacctgg
    11941 caccctgccc tatggagccc ggactttcct cccctccgcc cgtctccccc gaagaggacg
    12001 acgacaaagc ggcgactgtc tggtcagctt cggcgcgcag tatagagggt ttgcgcgccc
    12061 ttgtcacccc gtgttgcgca ttccaatcgc cagtgtcgcg gcaatattac gtgaagcacg
    12121 gcagatattg tcataagccc cgcggaatgc ttcgtccaac gtacctatgc tggtcaatac
    12181 gctgaagacc gcatcaatgc catgctgatg tacaacgcca acatcatcgg tcaggctacc
    12241 cgcacccgca atgccaataa ccggtttatg gtacttcttc gccacgtttg cgacgccaat
    12301 cggtaccttg ccgtgaatac tctggctgtc aatacgccct tcaccggtga tcaccagcgt
    12361 acaatcgtga atatgttcct ccagattcag cgccgtagtg acgatttcaa taccactttt
    12421 cagttccgca ccaagaaacg ccatcagcgc cgcacccata ccgcccgcag ctcctgcgcc
    12481 ggggacatct tttacatcaa catgcagcgc ttttttaatg acatcggcat agtgagagag
    12541 gttattgtcc agctcaacaa tcatcgcttc actggctccc ttttgtgggc caaagatgcg
    12601 cgatgcgccg ttatcgccca ccagcggatt ggtgacatca caagcgacgc gaatgacgca
    12661 atcttttaag cgcggatcga ggccggaaat atcaatatca ttcagagtat taagactacc
    12721 gccgccaaaa ccaatttcat tgccgttggc gtcgcataat ttcgccccca gcgcctgtac
    12781 catgcctgcg ccgccatcat ttgtagcgct accgccaatg ccgataataa tgtttgtcgc
    12841 gccgctctcc agcgcctgca ggattaactc gcctgtgccg cgtgaagtgg tcacgagtgg
    12901 atcgcgtttt tccgcaggta ccagctccag cccactggcc gccgccattt caataaacgc
    12961 ggttttgcca tcgccggaga tcccccaact ggcattcact ttctcgccca gcggccctgt
    13021 aacccaggtg tgacgttcag aaccctgggt ggctgcaatc atcgcttcca ccgttccttc
    13081 gccaccgtcg gcaaccggaa tagaaacgta ctgtgcatca ggaaaaattt cccgaaatcc
    13141 tttttctatc gcctgcgcaa cctcgctggc agataaactt tctttataag agtctggggc
    13201 gattacgatt ttcataccta tgcctgttac cacatgacgc cggagggcgt ttctcttatt
    13261 cggcctggat tccaggcccg gattgcaata cgccatccgg gcacgacgtc attaacgagt
    13321 aacttcgact ttcgccagtt tttcgtagta gcacgccagg gcgctatgat ccgccgttcc
    13381 taaaccatct gctcgcagtg cctgcatcat ctccataacc gcagctgtga gcggcagttg
    13441 cgcgccgacg ccgtgggaag tatccagcgc atttgccaga tccttaatat gcagatcaat
    13501 acggaagccc ggcttgaagt tgcggtccat caccatcggc gctttggcat ccagcacggt
    13561 actgcccgcc agtccaccgc gaattgcctg atacaccagg tccgggttaa cgcccgcttt
    13621 agttgccagc gttaacgctt ctgacatcgc ggcaatattc agcgccacaa tgacctgatt
    13681 cgccagtttg gtgacgttac ctgcaccaat tgccccggta tgcaccacgg aacccgccat
    13741 tgctttcatc aaatcatagt atttgtcgaa aatagccttg tcgccgccca ccatcactga
    13801 cagcgtaccg tcgatggctt tcggttcacc gccgctcacc ggagcatcca gcatatcaat
    13861 gcctttcgct ttcagcgctt cgctgatttc acggcttgcc agcggtgcga tagaactcat
    13921 atcgatcaat accgtacctg gcttcgcgcc ttcaataatg ccattctcac ccagcgccac
    13981 ctctttcaca tgaggggagt ttggcagcat ggttatgatg acgtcgcact gttcagcgat
    14041 cgctttagcc gtagaggctg tttctgcacc tgcagcaatc acgtcagcaa tagcttctgg
    14101 gttacggtca gcaaccacca gcgagtaacc tgctttcaga aggtttttac tcattggttt
    14161 acccataatc cctaggccaa taaaaccaac tttcatagtc atatcaatca tctctcttgt
    14221 tgcggtggtg gttatttttt aaaggtatca gccagtttat gagtggcaga gcggaagacg
    14281 ccgagatcgc tgccgacagc cacaaacgtc gcgccccatt ccagataacg acgcgcatcg
    14341 gcttcgaccg gcgcgaggat accgctgggt ttgccgtgcg cgctggcacg gttaaaaatg
    14401 tgctgaattg ctttttgtac atccgggtgt gatgcattgc cgagatggcc taatgccgcg
    14461 gccagatcgc tggggccgac gaagatgccg tctacgcctt cggtagcggc aatggcatcg
    14521 acgttatcta cgccctgctg actttctatc tggaccagaa tagtgatgtt cttgttcgac
    14581 tgagcgaaat aatccgccac ggtgccaaac atattggcgc ggtgagaaac ggagacgccg
    14641 cgaatgcctt ccggtgggta acgggttgat gccaccgcct gctctgcttc ctcttttgtt
    14701 tctacaaaag gaatcaggaa gttatagaaa ccgatatcca gaagacgctt aataattacc
    14761 ggctcgttgg tcggcactcg cactactggc gcgctggcgc tgcctttcaa ggccattaac
    14821 tgcggaataa acgtggagat atcgtttggc gcatgttcgc catccagcac cagccagtca
    14881 aacccagcca aaccaagaac ttcagtgcta atcgggttag agagtgctga ccagcaacca
    14941 atttgtacct gtttcgcagc cagtgcggct ttgaatttat tcgggaaaac atcgttattc
    15001 atcgcttata cctttgctta tttctgcaat tccatacgtt taatgtcgcc aactacgaag
    15061 aggtagcaga ccatcgccat cagcgctgaa catcccacga aaaccagtgc tgcattgaag
    15121 gagtgcagtt cacttaccag gtagccaatc accagtggag tgacaatgga ggcaacattg
    15181 ccaaagacgt taaagacgcc gccacagagg ccaacaatct ctttcggcgc ggtgtcagaa
    15241 atcaccggcc agcccagagc accaaatcct ttgccaaaaa aagccagcgc catcagcatg
    15301 accaccagcg tggtgttgtt ggtgtagtta cataagatga tggtggaagc cagcaacatt
    15361 cccagcacaa tcggtagctt acgtgccagg gtcagggata aaccgcgttt gatcagataa
    15421 tccgagaaga cacctcccag cacgccgccc gcaaaaccac acagtgccgg aatcgaggcg
    15481 accagaccca ctttcagaat cgacatgcct ttttcctgca ccagataaat cgggaaccag
    15541 gtgaggaaga accaggtgat ggtgttgata aaatattgtc cgaaaaatac gcccaacatc
    15601 atgcggttag agagcaattg cttgatgtaa tgcagtttgg gaccgcttgc tgccgcgctg
    15661 cccggctttt tgtggtccat atcgaccacc gcgccatttt cagagataaa cttcagctct
    15721 tcctcagaca tacgtgggtg atctgtcggg ttatgaatca acttgatcca cagcgccgtc
    15781 agcacaaaac caatcacccc cataacggta aagacgtgct cccatcccca ggcgaaagtc
    15841 agccagccaa gcagaggcga aaagagcgcc agcgagaaat attgtgccga gttaaagatg
    15901 gcggaggcag taccacgttc tttcgtcggg aaccaggcgg cgacaatacg ggcgttcgcc
    15961 gggaatgatg gagcttccga gaatcccagc ataaagcgca taaagaacat ggagatccct
    16021 gcccaggcca gcgggaacat atcaacaaag ccttgcagga aggtgaacag cgaccagaaa
    16081 aagaggctgt aggtgtaaac ttttttcgag ccaaacttat caagcaacca gccgccgggg
    16141 atttgcatca gcaagtaggc ccagccaaaa gcggagaaga tgtaacccat cgaaaccgca
    16201 cttaactgca actcttttgc cacttcggta ccagcaatag acagcgttgc acgatcggcg
    16261 tagttaacgg cggtaacaat aaaaataatc agtaatatta aatagcgggt atgcacacct
    16321 ttcctttttt cgtcaacggt gtccagaatc attttattta cctcgggtac ttatgctgat
    16381 ttttattatt atggggaagg tgttatttat aagtttcatt tatgccgtaa cgacaatgaa
    16441 ctcgggaatt agtataagca gcgcgagaat aataatcatt gtgcaaatgc taatttaatt
    16501 aatacttttt aaatattatt ttgagcatat gcacataagg ttgcgcgcta aagcacagat
    16561 ttgcgcttta ccttgccggg cggcactgca atccctgaaa tgattgacat tgatcacatt
    16621 tctgcgttta aactcctgac attcttattt cacccaatga agtcatttat ttttaaatga
    16681 gaccaggtcc tcattttaat aacccctggc tggagaatat tgcacaatgg ccaacatcga
    16741 aatcagacaa gaaacgccaa ctgcgtttta tataaaagtt cacgacacag ataatgtggc
    16801 aattattgtt aatgataatg gcctgaaagc aggaacgcgt tttccggatg ggctggaatt
    16861 aattgaacat attccccagg ggcataaagt cgcattgctg gacattccgg ctaatggtga
    16921 aattattcgt tatggcgaag tgattggtta cgccgtgcgt gcaatcccac gcggaagctg
    16981 gatcgacgaa tcaatggttg tactaccgga agcgccgccg ttacacacgc tgccactggc
    17041 aaccaaagtc ccggaaccct taccgccgct ggaaggatac acctttgagg gctatcgcaa
    17101 tgccgatggc agcgtgggca ccaaaaacct gctcggcatc accaccagcg tccactgtgt
    17161 ggcaggcgtg gtggactatg tagtaaaaat cattgaacgc gatctgctac cgaaataccc
    17221 gaacgtcgat ggcgtggtgg ggctgaatca tttgtacggt tgtggcgtgg cgattaacgc
    17281 accggcggca gttgtaccta ttcgtaccat tcacaatatt tcgctgaacc ctaactttgg
    17341 cggcgaagta atggtgattg gcctgggttg tgaaaagttg cagcctgagc gcctgctgac
    17401 tggaacggat gatgtgcaag ctattccagt agaaagcgcc agcattgtca gtttgcagga
    17461 tgaaaagcat gtcggttttc agtccatggt cgaggatatt ttgcaggtcg ccgaacgcca
    17521 tctacaaaaa ctgaatcaac gtcagcgaga aacctgtccg gcttcagaac tggttgtcgg
    17581 catgcagtgc ggtggcagcg atgcgttttc tggcgtaacg gcaaacccgg cggttggcta
    17641 tgcgtctgat ctactggtgc gctgcggcgc aacggtgatg ttttcagaag tcacggaagt
    17701 gcgtgacgcg atccatctgc tgacaccacg cgcagtgaac gaggaggtcg gcaaacggct
    17761 gctggaggag atggagtggt acgataacta tctcaatatg ggaaaaaccg accgcagcgc
    17821 caacccttcg ccgggcaaca agaaaggcgg tctggcaaac gtggtagaga aagcgctcgg
    17881 ctccattgct aaatcgggca aaagtgcaat tgttgaagtg ctgtcgcccg gtcaacgccc
    17941 gactaaacgc ggattaattt acgccgcgac gccagccagc gattttgtct gtggcacgca
    18001 acaggtggct tcgggtatca ccgtgcaagt gtttacgacc ggtcgtggta cgccgtacgg
    18061 cctgatggcg gtacccgtca ttaaaatggc aacccgcacc gagctggcga accgctggtt
    18121 tgatttaatg gatattaatg cgggcaccat cgctaccggc gaagaaacta ttgaagaggt
    18181 gggctggaag ttgttccact ttattctcga cgtcgccagc gggaagaaga aaaccttctc
    18241 ggatcaatgg ggactgcata accagctggc ggtgtttaac ccagcaccgg tgacctgata
    18301 attcaattcg tattatgccc gatagcacac gtttatcggg catattgagt cacagcgaag
    18361 acgcgtgatc cccgaccaga atcacttcaa ccccagcctt tcgcagtcct tccagactat
    18421 ccgcaggaat gccttcatca acaatgatca tgtcgatacg ttgagtatca atgatcttat
    18481 gtaaactgga acgattgaat ttactggaat cggtgaccac gatgatccgt tccgcaactt
    18541 cgcacatccg acggtttaaa cgggcttcat cttcattatg tgtgctgacg ccgcgctcca
    18601 gatcgatcgc atctacacca agaaacagca tatcgaagtg gtaattttgc agcgattgct
    18661 cagcctgatc gccgtaaaaa gattgcgact gacggcgcaa atgcccgccg gtcatcagca
    18721 gctcaacgcc ttccgcttcc agcaacgcat tagccacgtt cataccgttg gtcatcgcaa
    18781 ttacgtcagt gtacttgcgc atcagacgag caatctcaaa agtggtggtc ccggaatcga
    18841 ggatcacccg atgacctggc tgaatcaact caacggcagc tttcgcaacg ctgcgtttca
    18901 tcgcggtgtt cagtgcgctt ttatcttcca ctgatggctc gactgacggc gtcgtgctat
    18961 cgcagatcaa cgcgccacca taggcacgca cagcgatccc ctgcttttcc agaaaagcca
    19021 gatcattgcg gatcgtcaca gtagatacgc catacaatgc cgacagatcg ttaacctgca
    19081 cactcccttg ctgtcgcaga cgctgaatga tctgttctcg tcgctcgctg gtgcctgtca
    19141 ctcgcttctc acctgaagcg tcggtattac tcatagtaag tcctttcgta aaactttcgt
    19201 ttcatttcgt tttgcctatt aacgcctttc tattaagcaa atgcaagccc atcttgccca
    19261 ttggcgcaag ctactctcgt ttcagtgact ttcattatgt ttcttttgtg aatcagatca
    19321 gaaaactatt atatttcgtt ttatttttat ctcaccatga tgcagtatta attgaaacaa
    19381 aacgaaagat taatatcgca gtaatctgaa ctggagagga aagtgaaaca tctgacagaa
    19441 atggtgagac agcacaaagc gggcaaaaca aatgcaattt atgccgtttg ttccgcacat
    19501 ccgctggtgc tggaggctgc aatccgctac gccagtgcaa accaaacgcc gttactgatt
    19561 gaagcaacct ccaatcaggt agaccagttc ggcggttata ccggaatgac gcccgccgat
    19621 tttcgcggct ttgtttgtca gctcgccgac tcgttgaatt tcccgcagga tgcgctgatt
    19681 ctgggtggtg accatctggg gccaaaccgc tggcaaaacc tgccagccgc tcaggcaatg
    19741 gccaatgccg atgatttgat taaaagctac gttgcggcag gattcaaaaa aatccacctt
    19801 gattgcagca tgtcctgtca ggacgatcca attcccttaa ctgatgacat cgtggctgaa
    19861 cgcgccgccc gtctggcgaa agtggcggaa gaaacctgtc gcgaacactt tggcgaagcc
    19921 gatctggagt atgtcattgg taccgaagtg ccggtacctg gcggcgcgca tgaaacctta
    19981 agcgagctgg cggtcaccac gccggatgcc gcccgcgcca cgctggaagc ccatcgtcac
    20041 gcttttgaaa agcaaggttt gaatgccatc tggccacgca tcattgccct ggtggttcaa
    20101 cccggcgtcg aattcgatca caccaacgtt attgattatc agcccgccaa agcgagcgcc
    20161 ttaagccaga tggtcgaaaa ctacgaaacg ctgattttcg aagcgcactc taccgattat
    20221 caattgtttt aagccggatt gatgaacgct tgattcacgg tcaggtcggc gttcagtggg
    20281 tcggatttgc gggggcaaat ctggtgctgg tagccaacga tgaggttgcc gaagatccgg
    20341 tacaacaaaa cctgatggaa atggtactgg cagaagggat cgccgtacgt ttctggacgc
    20401 tgcaaaaagt tatcgacaac attcatcgcg ccgccgatcg acagaaaatc ctgctggttt
    20461 gtaaaacacc cgccgatttc ctgacgctgg tgaaaggtgg cgttccggtg aatcgcatta
    20521 acgttggcaa tatgcactac gccaatggca aacaacaaat cgccaaaacg gtttctgtgg
    20581 atgcgggcga tatcgcagca tttaacgacc tgaaagccgc tggggtggaa tgcttcgttc
    20641 agggcgtccc gacagagcct gctgtggacc tctttaaatt actttgaggg attcatcatg
    20701 gaaatcagcc tgttgcaggc atttgcgttg ggcattatcg cctttatcgc tggcctagat
    20761 atgtttaacg gcttaaccca tatgcaccgc ccggtggtcc tcggcccgtt ggtcgggctg
    20821 gtacttggcg atctgcatac cggaatttta accggcggta cgctggaact agtgtggatg
    20881 gggctggccc cgctggcggg cgcacagccg cctaacgtga ttatcggtac tatcgtcggc
    20941 acggcgtttg ccattactac tggcgtgaaa cccgatgtcg cggtcggtgt cgccgtacct
    21001 ttcgctgtcg cagtacagat ggggattacc ttcctgttct cggtgatgtc tggcgtgatg
    21061 tctcgctgcg accggatggc ggagaacgcc gatactcgcg gcattgaacg cgtgaactat
    21121 ctggcactgc tggcactcgg cactttctat tttctctgcg ctttcctgcc tatctacttc
    21181 ggcacggaac atgcaaaaac catcattgat gtcctgccgc aacgattaat cgacggcctc
    21241 ggcgtcgcgg gcggcatcat gccagcaatc ggttttgccg tgctgctgaa aatcatgatg
    21301 aaaaacgtct acatacccta cttcatcctg ggctttgttg ccgcagcctg gctcaagtta
    21361 ccggtgctgg ctattgctgc cgccgcgctg gcgatggcgc tgatcgatct gctgcgtaaa
    21421 tctcctgaac cgactcaacc tgcggcacag aaagaggaat ttgaagatgg catctaatca
    21481 aaccaccctg ccgaacgtca gcgaaaatga agaaacactg ctgactggcg tcaatgaaaa
    21541 cgtgtatgaa gatcggagca ttgccgcgga gctgacgaaa aaagatatca accgcgtcgc
    21601 ctggcgttcc atgctgttac aagcgtcttt taactacgaa cgtatgcagg cttctggctg
    21661 gttgtacggt ctgctgcctg ctctgaaaaa gatccacact aataaacgcg acctggcgcg
    21721 cgccatgaag gggcatatgg gtttcttcaa tacccatccg tttctggtga cgtttgttat
    21781 cggcattatc cttgcgatgg agcgttctaa gcaggacgtt aacagtattc agagcaccaa
    21841 aattgccgtc ggtgcgccgc ttggcgggat tggcgatgcg atgttctggc taacgctact
    21901 gccgatttgt ggcgggatag gcgcaagcct cgcgctacaa ggctctattc ttggcgcagt
    21961 cgtctttatt gtgctgttca acgtggtaca cttggggctg cgttttggtc tggcgcatta
    22021 tgcttaccgc atgggcgtgg cggcgattcc ccttattaaa gcgaatacca aaaaagtcgg
    22081 ccatgctgcg tcaatcgttg gaatgacggt aatcggcgca ctggtggcaa cctatgttcg
    22141 tttaagcacc acgctggaaa tcacggcggg cgacgcagtg gttaagttac aggctgatgt
    22201 tatcgacaaa ctgatgcctg ccttcttacc gctggtctac accctgacca tgttctggct
    22261 ggtacgccgc ggctagagtc cgctgcgcct gattgcggtt accgtggttc tagggatcgt
    22321 cggtaaattc tgccatttcc tttaaataca atgaggtatt agatgttaag tattattttg
    22381 acagggcatg gcgggtttgc cagtggcatg gaaaaagcaa tgaagcaaat cctcggcgat
    22441 caatcgcagt ttattgccat cgattttccc gaaacctcaa gcgccgcgct gcttacatcg
    22501 cagcttgaag aggccatcgc ccaactggat tgtgaagacg gcattgtttt tctgacggat
    22561 ttgctgggcg gcacgccgtt tcgcgtagct tcaacgctgg cgatgcaaaa accgggctgt
    22621 gaagtgatta ccggcaccaa tttgcaactg ctactggaga tggtgctgga gcgcgaaggg
    22681 ttaagcggtg aagaattccg tgtacaggcg ctggagtgtg gacaccgagg gctgaccagt
    22741 ctggtggacg agttaggccg ctgccatgaa gaatatccgg tcgaggaagg aatatgacac
    22801 acgttctgcg cgccagaagg ctgctgactg aagagggatg gttcgatgac catcagttgc
    22861 gtgttgctga cggtgtcatc gcagcaatcg aaccgattcc agtgggcgtg actgaacgcg
    22921 atgcggaact gctctgcccc gcttacatcg acacccatgt acacggtggt gcgggcgttg
    22981 atgtaatgga tgacgcgccg gatgtactcg acaagctggc aatgcacaag gcacgcgaag
    23041 gtgtcggcag ttggttaccg accaccgtaa ccgcgccgct taataccatt catgcggcgc
    23101 tgaaacgtat tgctcaacgt tgccaacgcg gcggacctgg tgcgcaagtg ctggggagtt
    23161 atctcgaagg accgtacttc acgccgcaga ataaaggcgc gcat
//
LOCUS       DAJTYF010000057        22589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTYF010000057 DAJTYF010000000
VERSION     DAJTYF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.057"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..1071)
                     /locus_tag="OO590_002317"
     CDS             complement(<1..1071)
                     /locus_tag="OO590_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6637664.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLS"
     gene            1570..2124
                     /gene="pinE"
                     /locus_tag="OO590_002318"
                     /pseudo
     CDS             1570..2124
                     /gene="pinE"
                     /locus_tag="OO590_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2463..3311)
                     /locus_tag="OO590_002319"
     CDS             complement(2463..3311)
                     /locus_tag="OO590_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6637665.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3520..4155
                     /gene="yfhb"
                     /locus_tag="OO590_002320"
     CDS             3520..4155
                     /gene="yfhb"
                     /locus_tag="OO590_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6637666.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4180..4716
                     /gene="tadA"
                     /locus_tag="OO590_002321"
     CDS             4180..4716
                     /gene="tadA"
                     /locus_tag="OO590_002321"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6637667.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(4713..6269)
                     /gene="mltF"
                     /locus_tag="OO590_002322"
     CDS             complement(4713..6269)
                     /gene="mltF"
                     /locus_tag="OO590_002322"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6637668.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6527..10414
                     /gene="purL"
                     /locus_tag="OO590_002323"
                     /gene_synonym="purI"
     CDS             6527..10414
                     /gene="purL"
                     /locus_tag="OO590_002323"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6637669.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10972..12399
                     /gene="qseE"
                     /locus_tag="OO590_002324"
     CDS             10972..12399
                     /gene="qseE"
                     /locus_tag="OO590_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6637670.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12564..13277
                     /gene="qseG"
                     /locus_tag="OO590_002325"
     CDS             12564..13277
                     /gene="qseG"
                     /locus_tag="OO590_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6637671.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13267..14601
                     /gene="glrR"
                     /locus_tag="OO590_002326"
     CDS             13267..14601
                     /gene="glrR"
                     /locus_tag="OO590_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6637672.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14662..15000
                     /gene="glnB"
                     /locus_tag="OO590_002327"
     CDS             14662..15000
                     /gene="glnB"
                     /locus_tag="OO590_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6637673.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15045..16235)
                     /gene="hmpA"
                     /locus_tag="OO590_002328"
     CDS             complement(15045..16235)
                     /gene="hmpA"
                     /locus_tag="OO590_002328"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6637674.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16562..17815
                     /gene="glyA"
                     /locus_tag="OO590_002329"
     CDS             16562..17815
                     /gene="glyA"
                     /locus_tag="OO590_002329"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6637675.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(18013..19206)
                     /locus_tag="OO590_002330"
     CDS             complement(18013..19206)
                     /locus_tag="OO590_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6637676.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            19324..19497
                     /locus_tag="OO590_002331"
     CDS             19324..19497
                     /locus_tag="OO590_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637677.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            19957..22589
                     /locus_tag="OO590_002332"
                     /pseudo
     CDS             19957..22589
                     /locus_tag="OO590_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380005.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
BASE COUNT         5403 a         5616 c         6016 g         5554 t
ORIGIN      
        1 ggaaaggcgg tcaaggaacg cggagaatgc ccgggaattc tcctcggtgg aaaaagaagt
       61 ccattgtgta gaaacctccc gacgatctgc ttcaggaaac caggctgcca cagtttcggg
      121 aagagggggg atctgaaagt tctgcccccc ttcagtgatg actctgatat ggcgcgaatg
      181 taaattttgc ggcaacatct ggcgtaaaaa cagtctgttt tggtctgaca gggaaatacg
      241 aaactgaggc ccgttaaaat ttggaaccga agtttgctgt attaagaata gcagagtttg
      301 atctgataag gggttatttg caatctcaat agttatatga ggatctaaac gaaaaagatt
      361 tgtcgggatt gtagttattt ggttgttttc aaaataaaaa aatcttctca tgataatatt
      421 ttcttggatg tttggtaagt tgcttaatct attgctatat gcaaaaagaa gttgcagtct
      481 cctaggtaaa gggggcagtt cagtaagatg gttgtgcgca accttaagta tcttaagatt
      541 agaaggcaga gatggaagag tttgcagttc gttgttccta acctcaagtt tttttaaatt
      601 caagggtaat gaaggcagtg tggataacct attatactct gcgcttagtg atttgatggt
      661 gtctggtaat aacggcagat tctctaattg attttcggat acatccaaag ttattagata
      721 atgtggtaat gcagatatat gtgttaagaa attatctctt gcaataagtt ctttaatatt
      781 ttcaggtaaa tcagtaagga tggttaattt attacagctc acatcaagct ttttaatgca
      841 agcagggagt acaggtaacg aggaaagcgc caaggctcgc aggtccagtt tatgcccctt
      901 agaggtaaga catgcacgaa gtcttcttac cgcctcagta cgctgttctt ccggtaaccc
      961 ctgtttctcc caatcatccc attcggacaa ataatggttg ggggatgtaa ttgtgctgat
     1021 atgtggaaat aaacaggatg aaatatttct gagcatatta atttctcgca tatggatatg
     1081 gaattagatt gtgaataaat tgtatttaat gtgtctctcc taatgaggag ggtgtttcag
     1141 aatattcgcc ataagggaat gagtgttata tcatgctttc taccctggta agtatatttg
     1201 cttttttcgt atatttggtt catctttttt tgatgtttta catattcaca atgcagagga
     1261 tttataccag aagacattag tcttgagtaa gatactacaa catctccaac tattttagcc
     1321 agcctgacta cttgacttgg taatatttgt tgctaataat catgtataaa ataagcttat
     1381 atacatagta acttcagcaa aggatttagt aatgatccaa atcatatctc aatgtactgt
     1441 ttttttcttt aaaaaaacag tacattctat tttataatga ctgtgtattc ccatggaagg
     1501 agatcaccca attctccccc cccccccccc gacttttgac gcagtgtctt gttaacaggg
     1561 ggatttacca tgcttattgg ctatgtacgc gtatcaacaa atgaccagaa taccgatcta
     1621 caacgtaatg cgttgaactg tgcaggatgc gagctgattt ttgaagacaa gataagtggt
     1681 acaaagtccg aaagaccggg gctgaaaaaa ctgctcagga cattatcggc aggcgacaca
     1741 ctggttgtct ggaagctgga tcggctgggg cgcagtatgc ggcatcttgt cgtgctggtg
     1801 gaggagttgc gcgaacgagg catcaacttt cgtagtctga ctgattcaat tgataccagc
     1861 acaccaatgg gacgcttttt cttccatgtg atgggtgccc tggctgaaat ggaacgtgaa
     1921 ctgattgttg aacgaacaaa agcaggactg gaagctgctc gcgcacaggg acgaattggc
     1981 ggtcgccgtc ccaaactttc accagaataa tgggcgcagg ccgggcgact aattgcatca
     2041 ggagtttctc gccagaaggt ggcgatcatc tatgatgttg gcgaatcgac tttgtataag
     2101 aggtttccag ccagggttat ataaaaccgc agacacggcg tatacaagga cgtgctacgg
     2161 ttgactgaaa agttaatgtt gtatgtcaca ggccttttgc gccgtaaagc cccgtccttt
     2221 agggcgcgga ggacgttaaa gaaagtggtg ttctttgtgt tgctgaactt atgcaaaatt
     2281 tttttgcacc ataaatccaa caacacattc aataacacat tgctgtatca ctatgaaaat
     2341 aaatgtgttt ttatttgatt tcacacatat cacaattgat gcatttttta gtgattaaca
     2401 gcgccataag gaaactctca aaacaacaca aaccgggcgg gcattatacg cgcattcttt
     2461 actcagacca gttttttcac cagcgcttca ctatgacgaa tacgttctgg tgccagttcc
     2521 agatcctgct gaatcagcgc aatgaacagc aaatccgtta acattccctg agcgtgacaa
     2581 gcagagattg aagcactgtt tgtcgcttgt tcttcggcaa tggtatacag gcaatgagaa
     2641 gcacgctgtt gcagggcatt cggagtaaag ccggtaatcg ccagcacttt tccgcccact
     2701 cgcagcatct catctgccgc caggtttaac tcgcgtcgta caccggtgta ggaaatggct
     2761 aataacagat cgtcagggga cgacgcctgt actgttgcga gtagcgcatg catatcgcgc
     2821 agtgcggcag cattgaagcc aatcttcatc agcttccagg caaagttttg cgccaccaga
     2881 cccgaagcgc caataccggt cagaattatc cgccgcgcag agcgcaacat tgttacgcat
     2941 tcatgcagtt tctcttcact attaacgttt agcgttgcgt acatagcggc agtattttct
     3001 ttaatcagtt tttcgccgac cagccgtaac ggatcatcac cgcggatttg gttatgaatg
     3061 ggcacggagg gcgattccgg ctggcttgcc agcgcttcac tcaacgccaa cttaagcgcc
     3121 ggaaaacctt tatagccgag tttttgcgcg aacttcacga cgctggactg actgactccg
     3181 gcttcgttgg ccagttgctg agagcttaaa tggcgcgccg tatcaggttg tagcagcaga
     3241 taatccgcca gttttttatc actctgggca agcccctggt aacgctgacg gatacgaagt
     3301 aagccgttca tatctttcct cgcgatgaaa tagtggcgac aacaggcaat atgagacaca
     3361 cgcgagtgaa ttttatattc cattgccgct aattgccatg aattaaaaat tccagttagc
     3421 catgatgcgg tcgattgttg ccggaaaagt gatgatctat ctgttaggct atgtagtagc
     3481 gaaaattaat caggatgttt cagtccagag gagtatggtt tggcaactca cgagcgtcgt
     3541 gtggtgtttt ttgacttaga tggaacattg catcagcagg atatgttcgg cagttttctg
     3601 cgctatttac tgcgtcgcca accgctgaat gcgttacttg tcctgccgtt gttaccgatt
     3661 atagccattg cgttattgat aaaaggtcgt gcggcacgct ggccgatgag tctgcttctg
     3721 tgggggtgca cttttggtca cagcgaagca cgtttacaga cgttgcaggc cgatttcgtg
     3781 cgctggtttc gcgacaatgt taccgccttt ccgctggttc aggagcgatt aaccacctac
     3841 ctgttaagtt ccgatgctga tatctggttg attaccggct ctccgcagcc gctggttgaa
     3901 gcggtttatt tcgatacgcc ctggctgccg cgggttaatc ttatcgccag ccaaattcag
     3961 cgtggctatg gtggttgggt attgacgatg cgttgtctgg gacatgaaaa ggtcgcacaa
     4021 ctggagcgca aaatcggcac gccgctgcgg ctgtacagtg gctatagcga cagtaatcag
     4081 gacaatccgc tgctttattt ctgtcagcat cgttggcgag taaccccgcg cggtgaactc
     4141 cagcaactgg aatagagtaa agcatagcgt ccgtgtataa tgcgccgcgc ttttataacc
     4201 ggagttttct ttttgtctga agtcgaattt agccacgaat actggatgcg tcacgcgctg
     4261 acgctggcga aacgtgcctg ggatgagcgg gaagtgccgg tcggcgcggt attagtgcat
     4321 aacaatcggg taatcggcga aggctggaac cgcccgattg gtcgccatga tcccaccgca
     4381 catgcagaaa tcatggccct gcggcagggt ggtctggtga tgcaaaatta tcgtctgatc
     4441 gacgccacgt tgtatgtcac gcttgaacca tgtgtaatgt gtgccggagc gatgatccac
     4501 agtcgcattg gtcgcgtggt ctttggtgcg cgtgacgcga aaactggcgc tgcgggatct
     4561 ttaatggatg tgctgcatca tccgggtatg aatcaccgag tggaaattac ggaaggaata
     4621 ctggcggatg agtgcgcggc gttgctcagt gacttctttc gcatgcgccg ccaggaaatt
     4681 aaagcgcaga aaaaagcgca atcctcgacg gattaatttt gtttctcttc actccctttc
     4741 ctggaaaaca gcagagaggg agaatgggtc aaatagtttg atgacgactg ggaaagaaac
     4801 gagagaaaag gaaatttctc tttgcccaac tccgtaggcg ataccgccgg ataatcctgc
     4861 gccagttgca tcgccgcttc tgtagcctgc ttctctttct cttgcagata acccaccagg
     4921 ctaatctgat acttacgaat attttcgaca taagcgtagg cttcatgccc acgagcgtag
     4981 ccgtaagtca gcttgctgta atagggtttc tggctaagta aaggcagacg ctgttttacg
     5041 tcagcccaac tgtcaggatt ccctttggtt tttgccgtca gggcgcgggc atccagcata
     5101 tgcgcatagc ccatattgta cgcagcgagg gcaaaccaga tccgttcgtt ctccggcaca
     5161 ctttccggca ctttactcat catatcctgc aaataacgca cgccaccgct gatgctctgt
     5221 tcggcatcgg tacgatccgt aatgccgagg ctttgcgcgg tatttttggt taacatcatc
     5281 atgccgcgca cacccgtcgg tgaagtggcc tgtgcatccc agtgcgattc ctgataagca
     5341 atagcggcca gcaaacgcca gtcaatttct tcggcgtatt tctcaaacag gggctttaac
     5401 tgcggcagta ccgcatcgac ggcgcgtaaa aatgtgcgcg tatcgacgta atcaaaatca
     5461 tcgccatgcc ccaggtattt ctcttcaatg cgtgccagcg taccgtcttc attcatttcg
     5521 ttgaagaagt cgagcagggc ggcggaaagg gtattatcgc catctaacgg gctaaaccaa
     5581 gtcaccggtt gttcatcggt gatatcgagc gctacggcga gctccgggtg aacgcgctga
     5641 aacaggctga tggcgacaga atcagcaatg gtgtaatcga gttttccttc gatgacatct
     5701 tccattaatt ccgcagagcc ttttttgtcg tctaccttcc agcttaattc cgggaatttt
     5761 gtttctttca gggtctggag atcgttaacc accacatgac ccggtgcaac ggtgagttgc
     5821 tccgccgtca ggttgcccag cgtacgtggg cgatactgac ccactttata aaccagttgt
     5881 tgtgacacgg aataataggt agggccaggc tgataatttt ttacccgctc actgttatag
     5941 accagtcctg ccgccagcag gtcggcgtta ccattatcaa ggtcgtcaaa cagctggctg
     6001 atattctgcc gcacggtcac tttcagtttt acgccgaggt aatcggcaaa ctgtttcgcc
     6061 agttcgtaat ccaggccaaa aggtttcccg ttgatttcgt tataagtcag gggagtatga
     6121 atggtgctca cacgcaactc tccccgcgct tgaatggcgg cgatacggtt gtcggcttta
     6181 ccaaaccagg gaatggatgg ccagagagcg accgcgagca gcagtgccag aatgccgatg
     6241 aacagataat taatctttaa ttttttcaat tagttaattc tctgtgtcgt gcgcgtccca
     6301 gcttgaaaaa acgtaataat agtgaaaggt ttactcataa atgagcggca ttttgcgtaa
     6361 acctgcgcca gatggcaact tattacagcc attggcggca cgcgttgcta attcacgatg
     6421 gtgattttat ttccacgcaa acggtttcgt cagcgcatca gattctttat aatgacgccc
     6481 gtttcccccc cttgggtaca ccgaaagctt agaagacgag agacttatga tggaaattct
     6541 gcgtggttcg cctgcactgt cggcattccg aatcaacaaa ctgctggcac gttttcaggc
     6601 tgccaggctc ccggttcaca atatttacgc cgagtatgtc cattttgctg acctcaatgc
     6661 gccgttaaac gatgatgagc acgcacaact tgaacgcctg ctgaaatatg gtccggcact
     6721 cgccagccac gccccgcaag gcaaactcct gctggtgacc ccgcgtcctg gcaccatctc
     6781 tccctggtct tcgaaagcga ccgatattgc ccataactgc gggctacaac aggtaaaccg
     6841 ccttgagcgc ggcgttgctt actatataga agccggtacg ctgaccaatg aacaatggca
     6901 gcaggttacc gctgaactgc acgaccgcat gatggaaacg gtcttttttg atttagatga
     6961 tgcagagcag ttgtttgccc accatcaacc gactccggtc acaagcgttg atttgctggg
     7021 gcagggccgt caggcgctga tcgacgctaa cctgcgtctt ggcctggctc tggcggaaga
     7081 tgaaattgac tatctgcagg atgctttcac aaagcttggt cgtaacccga acgacatcga
     7141 gctgtatatg ttcgctcagg cgaactctga gcactgccgc cacaaaattt ttaacgccga
     7201 ctggattatc gacggtgaac aacagccgaa atcgctgttc aggatgatca aaaatacttt
     7261 cgaaaccacg ccagatcacg ttctctctgc ttataaagat aacgccgccg taatggaagg
     7321 ttctgaagtg ggccgctact ttgctgacca cgaaacgggc cgctacaatt tccatcagga
     7381 accggcgcac atcctgatga aagtcgaaac tcacaaccac ccgacggcga tttctccgtg
     7441 gccgggcgcg gcgaccggtt ccggcggtga aatccgcgat gaaggtgcca ccgggcgcgg
     7501 cgcgaagccg aaagctggtt tggttggttt ctccgtatcc aacctgcgaa ttcctggctt
     7561 cgaacagccg tgggaagaag atttcggtaa gcctgagcgc attgttaccg cgctggacat
     7621 catgaccgaa ggcccgctgg gcggtgcggc gtttaacaac gaatttggtc gtccggcact
     7681 gaacggctac ttccgtactt atgaagaaaa agtgaacagc cacaatggcg aagagctgcg
     7741 cggttatcac aaaccgatca tgctggcggg cgggatcggc aacattcgcg ccgatcacgt
     7801 acaaaaaggc gagatcaacg tcggtgcgaa gctggtcgtt ctcggcggcc cggcaatgaa
     7861 catcggtctt ggcggcggcg cggcgtcttc catggcgtct ggtcagtctg atgccgacct
     7921 cgactttgct tccgtacagc gcgacaaccc ggagatggag cgtcgttgcc aggaagtgat
     7981 cgaccgttgc tggcagcttg gtgatgctaa cccaattctg tttatccacg acgtcggtgc
     8041 gggcggtctt tctaacgcga tgccggagct ggtgagcgac ggcggacgcg gcggtaaatt
     8101 tgaactgcgc gatattctaa gcgacgaacc gggcatgagc ccgctggaaa tctggtgtaa
     8161 cgaatcccag gaacgctacg tgctggcggt tgctgccgat caattaccgc tgtttgacga
     8221 actgtgtaag cgtgagcgcg caccctacgc ggtgattggt gaagcgaccg aagaactgca
     8281 tctttctctg cacgatcgtc attttgataa tcagccgatc gatctgccgc tggacgtcct
     8341 gcttggtaaa acgccgaaga tgacccgcga tgtacaaacg ctgaaagcga agggcgatgc
     8401 gctggtccgt gaagggatca ccattgcaga cgcggtgaaa cgcgtactgc atctgccgac
     8461 cgtggcggag aaaactttcc tggtgaccat cggtgaccgc agcgtaaccg gcatggtggc
     8521 gcgcgatcag atggtggggc cgtggcaggt gccggtcgct aactgcgcgg tcactaccgc
     8581 cagcctcgac agctactacg gtgaagcgat ggcgattggc gagcgtgcgc cggttgcgct
     8641 gctggatttc gccgcctctg cccgtctggc ggtcggtgaa gcgttaacca atattgccgc
     8701 aacacagatt ggcgatatca aacgcattaa actttccgcc aactggatgg cggcggcagg
     8761 ccaccctggt gaagatgcgg gcctgtatga agccgttaaa gccgtgggtg aagaactttg
     8821 tccggcgctg ggcctgacga tcccggtggg taaagactct atgtcgatga aaacccgctg
     8881 gcaggaaggt aacgaagagc gcgaaatgac gtcgccgctg tcgctggtga tttctgcatt
     8941 tgcccgcgtg gaagatgtac gtcacaccat cacgccgcag ctttgtaccg aagataacgc
     9001 actgctgctg attgatttgg gcaaaggcaa taacgcgctg ggcgcaacgg cgctggcgca
     9061 ggtttatcgt cagcttggcg acaaaccggc agatgtacgc gatgttgtcc aactgaaagg
     9121 cttctatgac gcgattcagg cgctggttgc acagcgtaag ctgctggcgt atcacgaccg
     9181 ctctgatggc ggcctgctgg taacgctggc ggaaatggcc tttgcaggtc attgtggcat
     9241 tgacgcggat atcgcctctc tgggtgacga tcgcctggcg gcgttgttta acgaagaact
     9301 gggtgcggtg attcaggttc gtgccgctga ccgtgaagcg gtcgagtccg tactggcaca
     9361 gcatgggctt gcggattgcg tccattatgt tgggcaggcg gtttccggtg accgttttgt
     9421 gattaccgcc aacgggcaga ctgtattcag cgaaagccgc actacgttgc gtgtctggtg
     9481 ggcagaaact acctggcaga tgcagcgcct gcgtgacaac ccggagtgtg ccgatcagga
     9541 gcatcaggcg aaatctaacg acgccgatcc gggcctgaat gtaaaactgt cgttcgatat
     9601 caacgaagat gtggcagcac cgtatattgc cactggcgca cgtccgaaag ttgccgtact
     9661 gcgtgagcag ggcgtgaact cgcatgttga aatggcggca gctttccaac gtgggggctt
     9721 tgatgctatc gacgtgcata tgagcgacct gctgactgga cgtacgggac tggaggattt
     9781 tcacgccctg gtcgcgtgcg gaggtttctc ctacggtgat gtgctgggtg ccggtgaagg
     9841 ttgggcgaag tctatcctgt tcaatgaccg tgtacgcgat gagtttgcaa ccttcttcca
     9901 tcgtccgcaa acgctggccc tgggggtatg taacggttgc cagatgatgt ctaacctgcg
     9961 tgaactgatc ccggggagcg agttgtggcc acgttttgtg cgtaacacct ccgatcgctt
    10021 tgaagcgcgt ttcagcctgg ttgaagtaac ccaaagcccg tctctgctgt tgcaggggat
    10081 ggtgggctcg caaatgccga ttgctgtctc tcatggtgaa gggcgcgtgg aagtgcgtga
    10141 tgcggcgcat ctggcggcac tggaaagcaa agggctggtg gcactgcgct atgtcgataa
    10201 cttcggcaaa gtcactgaaa cctacccggc taacccgaac ggttcaccga acggtattac
    10261 ggcggttacg actgaaagtg gtcgagtcac cattatgatg ccgcacccgg aacgtgtttt
    10321 ccgtactgtc agcaactcct ggcatccgga aaactggggc gaggatggcc catggatgcg
    10381 cattttccgc aatgcgcgta agcagttggg gtaagtcatc agttcattgg ttttgcagcc
    10441 cggatgcggc attaagcacc aaccactatc ataagctccc aactctggga gcttttttgt
    10501 gtctgtaaat cacgacaatg ggtggtttac cgtgtcgctt tctggcgaca cttaaatgat
    10561 tgattttatt gtaatctaat aagtttatct taagttgtct ctttttagcg acacagtggc
    10621 tgatttacat tcaaatagcg aagaagccat aaatctctca aagcaacgta taatcagtac
    10681 gttaccaaac tattttcttt attggcacag ttactgcata atagtaagca gtggctcatt
    10741 caccgactta tgtcagcccc ttcgggacgt gctacataaa attcgaatga cgcacaacaa
    10801 ggtgcctgcc gtccaacttc tgatatcagc gtagctatat caaccatcgg gcgaaacgtc
    10861 gagttaggca ccgccttatt ccataacaaa gccgggtaat tcccggcttt gttgtatctg
    10921 aacttcccct cggttagcat caggctattc gcgtctgacg agagtaacac cttgaaacgc
    10981 tggcccgttt ttccccgctc attacgacaa ctggtaatgc tggcattttt gctaattcta
    11041 ctacccctgt tggtgctggc atggcaagcc tggcaaagcc tgaatgcgct tagcgatcag
    11101 gcggcgctgg ttaaccgcac tacgcttatt gatgcccggc gtagtgaagc gatgaccaac
    11161 gcggcgctgg agatggagcg tagctaccgt cagtattgcg tgctggacga ccctacgctt
    11221 gcgaaggttt atcaaagcca gcgcaagcgt tacagcgaaa tgctcgatgc ccacgcaggc
    11281 gtactgccag acgataaact ttaccaggca ttgcgtcagg atttgaacaa tctggcccaa
    11341 cttcagtgta acaacagtgg tcccgatgct gccgccgccg cgcgtcttga agcctttgcc
    11401 agtgccaata ccgaaatggt gcaggccacg cgcacagtgg tgttctctcg tgggcagcaa
    11461 cttcagcgtg aaatcgccga acgtgggcaa tattttggtt ggcaatcgct ggtgctattt
    11521 ctggtgagtc tggtgatggt gctacttttc acgcggatga ttatcgggcc ggtgaaaaat
    11581 atcgagcgca tgatcaaccg tctgggggaa gggcgttctc tgggcaatag cgtttcgttc
    11641 tgtggaccga gcgagttacg ctcggttggg caacgtattc tttggttaag tgagcgcctg
    11701 tcatggctgg aatcccaacg ccatcaattt ttaagacatt tatctcatga attaaaaacg
    11761 ccactggcga gtatgcgcga gggcactgaa ttactggctg atcaggttgt cgggccgctt
    11821 acgccagagc aaaaagaggt ggtgagcatt cttgatagca gcagctgcaa tttgcaaaaa
    11881 ctgatcgaac aactgcttga ttacaaccgt aaacaggcgg acagtgcggt ggaactggag
    11941 aatgttgaat tagcaccgct ggtggagaca gtggtttctg ctcatagcct gcccgcacgg
    12001 gctaaaatga tgcataccga cgtcgatctc aaagcaacag cttgcttggc ggagccaatg
    12061 ctgctgatga gcgtactgga taatctttac tccaatgcgg tgcactacgg ggctgaatcc
    12121 ggtaacattt gccttcgcag cagtttacat ggtgcgcggg tttatattga tgtcatcaat
    12181 acaggcacgc ccattccgca agaggaacgc gccatgatct tcgaaccctt ttttcaggga
    12241 agccaccagc gaaaaggggc ggtgaagggc agcggtctgg gattaagcat tgccagggat
    12301 tgtattcgcc gtatgcaagg ggaactgtat ctggtcgacg agagcgggca agacgtttgt
    12361 ttccgcattg aattaccgtc gtcgaaaaac acgaaataat gctaacagcc tatcctatca
    12421 ggctatttta tttgtcagtt cggccccggg tagtgctcga aatcttcacg tacgatatgt
    12481 acgccccggt ttctccgcgc tgtccatgtc cagactgtct gcaacaatta cgcccactgg
    12541 gataggcttt aagtctggtg aatatgcgac acatttttca acgattattg ccccgacggc
    12601 tgtggctggc agggctgcca tgtctggcat tgctgggttg cgtgcagaat cacaataagc
    12661 cagccattga tacgccagca gaagaaaaaa ttccggttta tcaactggct gactatcttt
    12721 ctactgaatg tagcgacatc tgggcgctgc aaggtaaatc aaccgaaacc aatccgcttt
    12781 actggctgcg ggcgatggat tgtgctgatc gtttaatgcc tgcgcagtcg cgtcagcaag
    12841 ctcgccaata tgacgacggc agctggcaaa atacctttaa acaagggatt ttgctcgccg
    12901 acgccaaaat tacgccgtat gagcgccgcc agctggtcgc gcgtattgaa gcgttaagta
    12961 ccgagatccc ggcacaggtt cgcccactgt atcaactctg gcgcgacggt caggcgctgc
    13021 aactgcaact ggcggaagag cgccaacgtt acagtaaact ccagcagtcc agcgacagcg
    13081 agctggatac attgcgccag caacatcacg ttctacagca gcaactggaa ctcaccaccc
    13141 gcaagctgga aaacctgacc gatattgaac gccagctctc gacccgcaaa ccggctggaa
    13201 atttctcgcc ggatacgccg cacgagagtg aaaagcccgc gccatccacc catgaggtca
    13261 ctcctgatga gccataaacc tgcgcattta ttattggtcg atgacgatcc gggattgctg
    13321 aaactgcttg gcctgcgcct gaccagcgaa ggctacagtg tggtcacggc ggaaagtggc
    13381 gctgaaggat tacgggtact gaatcgcgaa aaagtagatt tagttatcag cgacctgcgg
    13441 atggatgaaa tggacggtat gcagctattt gctgaaatcc agaaagtgca gccgggaatg
    13501 ccagtaatta ttcttactgc gcatggttct atccccgatg ccgttgctgc aacacagcag
    13561 ggcgttttta gtttcctcac caagcctgtc gataaagatg cgctatatca ggcaattgac
    13621 gatgcgctgg agcaatccgc gccagccacc gatgaacgct ggcgcgaggc aattgtcacc
    13681 cgcagcccgc tgatgctgcg tttgctggaa caggcgcggc tggtggcgca atcagacgtc
    13741 agcgttttga ttaacggtca gagcggcacc gggaaagaga ttttcgccca ggctatccac
    13801 aacgccagcc cgcgcaacag caaaccattt attgctatta actgtggcgc attgcccgag
    13861 caattgctgg agtcggagct gtttggtcat gcgcgtggcg cgtttactgg cgctgtcagc
    13921 aatcgcgaag gtttattcca ggcggcggaa ggcggaacgc tatttctcga tgaaattggc
    13981 gatatgcctg cgccgttaca ggtcaaactg ctgcgcgtgt tgcaggagcg taaagtgcgc
    14041 ccgctgggca gtaaccgcga tattgatatc aatgtgcgga ttatttctgc cactcaccgt
    14101 gatctgtcaa aagcgatggc gcgcggggaa ttccgcgaag atctctatta ccgcctcaac
    14161 gttgtcagcc tgaaaattcc ggcactggcg gagcgcacag aagacattcc gctactggca
    14221 aatcacctgt tgcgccaggc ggcagagcga cataaaccgt ttgtccgcgc gttctctacc
    14281 gatgcgatga aacgcctgat gaccgcgagc tggccgggta atgtgcgcca gttggtcaac
    14341 gtgattgaac agtgcgtggc gctgacctca tctccggtga ttagtgatgc gctggtggag
    14401 caggcgctgg agggtgaaaa tacggcgctg ccaacctttg ttgaggcacg taatcatttt
    14461 gaactcaact atttgcgtaa gctgctgcaa atcaccaaag gcaacgtcac ccacgcggcg
    14521 agaatggcgg ggcgcaaccg gacagaattt tataaactgc tttcccgaca cgagctggat
    14581 gcaaacgatt tcaaggaatg aattggcgtt atgtgttacg tttagcagat caaaagacag
    14641 acaacctttt caaggaatag catgaaaaag attgatgcga ttataaaacc cttcaagctg
    14701 gacgatgtcc gcgaagcact ggccgaagtc ggtattaccg gcatgacggt gaccgaagtg
    14761 aaaggctttg gtcgccagaa aggccatacc gagctgtacc gcggcgcgga gtatatggtg
    14821 gattttctgc cgaaagtgaa aattgagatt gtcgtaccgg acgacattgt cgatacctgt
    14881 gtcgatacca ttattcgcac ggcgcaaacc ggcaaaatcg gtgacggtaa aatcttcgtc
    14941 tttgacgtgg ctcgggtcat tcgcatccgt accggtgaag aggacgacgc ggcaatttaa
    15001 accgcgtcaa gggttgccgg atgtttccat ccggcaacat caaattacag caccttatgc
    15061 gggccaaagc attcgtaatg aatgttttcc tgcttcacgc ccagatccac taactgtttc
    15121 gcggtaaact gcatgaagcc aaccgggccg cagagataga actgcattgt cggatcgctg
    15181 aacgcacctt ccagtttgct caaatccatc agaccttcgc tatcaaactg acctttagcg
    15241 cgatcggctt cgctcggctg acgataccag gtgtgcgcgg taaagcgcgg cagtgactgc
    15301 cccagttcct taacttcatc agcaaaggcg tgaacttcgc cattttctgc cgcatggaac
    15361 cagttcactt gtgctgtgtg gcctgctttt gccagcgtgt cgagcattgc cagcattggc
    15421 gtttgaccaa caccggtaga gattaacgtc actggtgtgt catctgcgac agccataaag
    15481 aaatcacctg ctggagcgac cagtttcacg acatcgccaa cattggcgtg attgtgcaac
    15541 cagttggata cctgcccacc ctcttcgcgt ttcaccgcga tacgatagcc tttgccatcc
    15601 ggtttgcgag tcaaagagta ctgacgaatt tcctgatgcg ggaaaccttc cggcttcagc
    15661 cagacgccga gatattgccc cggacggtat tctgccactg cgccaccgtc gaccggctcc
    15721 agttcgaagc tggtgataag cgcgctgcgc ggtgttttag ccacaatgcg gaaatcgcga
    15781 gtaccttccc aaccaccggc tttgctggcg ttttcgttat agatttccgc ctcgcgattg
    15841 ataaatacat tagccagtac accataggct ttaccccacg cgtccagcac ttcctgcccc
    15901 gggctgaaca tttcgtccag cgttgccaac aggtgttcac cgacgatgtt gtactgttcc
    15961 ggtttgatct ggaagctggt gtgcttctgc gcgatttttt ctaccgctgg cagcagcgca
    16021 ggcaggtttt caatattact ggcgtaggcg gcaatagcgt taaacagggc ttcacgttga
    16081 tcgccattac gctggttact catgttaaaa atttctttga gttctgggtt atgagtaaac
    16141 atacggtcgt agaaatgggc ggttaacttt ggccccgttt ccaccagtaa agggatggtg
    16201 gcttttactg tagcgatggt ttgagcgtca agcatatggt cttccttttt tgcatcttaa
    16261 ttgatgtatc tcaaatgcat cttataaaaa atagccctgc aatgtaaatg gttctttggt
    16321 gtttttcaga aagaatgtga tgaagtgaaa aatttgcatc acaaacctga aaagaaatcc
    16381 gtttccggtt gcaagctctt tattctccaa agccttgcgt agccttaagg taatcgtttg
    16441 cgtaaattcc tttgtcaaga cctgttatcg cacaatgatt cggttatact gttcgccgtt
    16501 gtccaacagg accgcctata aaggccaaaa attttattgt tagctgagtc aggagatgcg
    16561 gatgttaaag cgtgaaatga acattgccga ttatgatgcc gaactgtggc aggctatgga
    16621 gcaggaaaaa gtacgtcagg aagagcacat cgaactgatc gcctccgaaa actacaccag
    16681 cccgcgcgta atgcaggcgc agggttctca gctgaccaac aaatatgctg aaggttatcc
    16741 gggcaaacgc tactacggcg gttgcgagta tgttgatatc gttgaacaac tggcgatcga
    16801 tcgtgcgaaa gaactgttcg gcgctgacta cgctaacgtc cagccgcact ccggctccca
    16861 ggctaacttt gcggtctaca ccgcgctgct ggaaccaggt gataccgttc tgggtatgaa
    16921 cctggcgcat ggcggtcacc tgactcacgg ttctccggtt aacttctccg gtaaactgta
    16981 caacatcgtt ccttacggta tcgatgctac cggtcatatc gattacgccg atctggaaaa
    17041 acaagccaaa gaacacaagc cgaaaatgat tatcggtggt ttctctgcat attccggcgt
    17101 ggttgactgg gcgaaaatgc gtgaaatcgc tgacagcatc ggtgcttatc tgttcgttga
    17161 tatggcgcac gttgcgggcc tggttgctgc tggcgtctac ccgaacccgg ttcctcatgc
    17221 tcacgttgtt actaccacca ctcacaaaac cctggcgggt ccgcgcggcg gcctgatcct
    17281 ggcgaaaggt ggtagcgaag agctgtacaa aaaactgaac tctgccgttt tccctggtgg
    17341 tcagggcggt ccgttgatgc acgtaatcgc cggtaaagcg gttgctctga aagaagcgat
    17401 ggagcctgag ttcaaaactt accagcagca ggtcgcgaaa aacgctaaag cgatggtaga
    17461 agtgttcctc gagcgcggct acaaagtggt ttccggcggc actgataacc acctgttcct
    17521 ggttgatctg gttgataaaa acctgaccgg taaagaagca gacgccgctc tgggccgtgc
    17581 taacatcacc gtcaacaaaa acagcgtacc gaacgatccg aagagcccgt ttgtgacctc
    17641 cggtattcgt gtaggtactc cggcgattac ccgtcgcggc tttaaagaag ctgaagcgaa
    17701 agaactggct ggctggatgt gtgacgtgct ggacagcatc aatgatgaag ccgttatcga
    17761 gcgcatcaaa ggtaaagttc tcgacatctg cgcacgttac ccggtttacg cataagcgaa
    17821 acggtgattt gctgacaatg tgctcgttgt tcatgttgga tgcggcatga acaccttctc
    17881 aggcctacaa aatcttgcaa attcaatata ttgcaatctc cgtgtaggcc tgataagcgt
    17941 agcgcatcag gcaatttttc gtttatgatc atcaaggctt ccttcgggaa gcctttctac
    18001 gttatcgcgc catcaaatct gtcgtaactg cgcctcaaca tacaaatagc caattcccag
    18061 cacctgttgt gcgcggctta attgcccgaa gccaatttgc gtcgctttca cgctcggtcc
    18121 ttcgtcgcgg tcgaacgggt taaatcctgt ctggcgaata atagtattaa gccagttctc
    18181 accaaaggca caactgcgac cgtacaacca aatctgatta atattgagga tgttgaggaa
    18241 gttatacaga ctcaaaccaa tggcattggc agagcgatca acccagctgg tgatccacgg
    18301 ttctccactc tgccaggcag cgattaactg cgcggttgtc agtttttcag gatcaagctg
    18361 agtattaacc ggttgtgatt ttagccatac ccgcgcctgt ttttttaatg cacttaacga
    18421 ggcgacggtt tccaggcagc cgtaacgtcc acagtcgcag acgacgccat ccggattaac
    18481 gatggtgtga ccaatctgtc cgctaccata caaactgccg cgataaattt gctcgttaat
    18541 cacgaacgac gagccaatgc cgtaatcaac gttgatcacg cagaaatccc gttcctgcga
    18601 attattttgc catttctccg cctgcgccag catcacgcag tcattatcga ccagcacccg
    18661 aatgcccagc ttctcttcca gcagatactt cacctcaact ggcgttgtcc acggcgcttg
    18721 cggcatggtt tgcgatacgc cggtcaccgg atcaacctgc ccgtggattg ccagcgccag
    18781 attgatggtg cggtccggcc acaatttacg gtggcgatgc cagcattttt cgatttcgga
    18841 cagcagcgcc tgcggcgtcg gtacatcaat ctgtaaatac tcaaattcgc ctttcggact
    18901 taaacaagca ttagcaacct gacactcaat actggtgggc gtcacgttca ggcacagcgt
    18961 ccagtcacct tccggcgcaa tcagccatgt accgctacta tgcccgcgcg tctggctttc
    19021 atcgtcaata ttcaccaccc gtttttcgct ttccagttcc tgcaaaatat tactgactgc
    19081 cggaatcgaa atttgcgcca gccgggccag cgttgattta ttggcgcgct tttgccggta
    19141 cagcagttcc agaatcacgc atttgttatg gtggcgaatc tgttgattgt taatgcaggc
    19201 tctcatcttc actaaacctt gtttagttaa ttgcgtgatt attccgcgaa aacattgcca
    19261 gattgttcaa tacactgcca caaatctttt aattcagtat gtcttgttaa tattgagggc
    19321 accatgactc cagtaaaagt gtggcaagag cgcgttgaga tcccgaccta tgaaaccggg
    19381 ccgcaggata tacatcccat gttcctggaa aatcgcgttt atcagggatc gtccggcgcg
    19441 gtttatccct acggcgtgac cgatacgctg agcgagcaga aaaccctgaa atcctagcag
    19501 gcggtgtggc tggaaaacga ctacatcaaa gtgatgatcc tgccggaact gggcggtcgg
    19561 gtgcatcgcg catgggataa agtgaaacag cgcgattttg tctatcacaa tgaagtcatt
    19621 aaacctgcgc tggtggggct gctgggaccg tggatctccg gcgggattga gtttaactgg
    19681 ccgcaacacc atcgcccgac cacctttatg cccgttgatt tcaccctcga agcccatgaa
    19741 gacggtgcac agacggtgtg ggtcggcgaa acggagccga tgcatggttt acaggtgatg
    19801 acaggtttca ccctgcgccc tgaccgggcg gcactggaaa tcgccagccg cgtctataac
    19861 ggcaacgcca cgccgcgtca tttcttgtgg tgggccaacc cggcagtgaa agggggtgaa
    19921 gggcatcaga gcgttttccc accggatgtg actgcggtgt ttgatcacgg caaacgggcc
    19981 gtctccgctt tccccatcgc caccggcact tactacaaag tggactactc cgccggagtg
    20041 gacatttctc gctataaaaa tgtgcctgtt ccaacctcat atatggccga aaaatcgcag
    20101 tacgattttg tcggcgcgtg gtgtcacgat gaagatggcg gtttgctgca cgttgccaac
    20161 caccatattg cgccaggtaa aaaacagtgg agttggggac acagtgaatt tggccaggcg
    20221 tgggataaga gtctgaccga caataacggc ccgtatatcg aactgatgac cggtattttt
    20281 gccgataacc agcctgattt tacctggctt gatgcttacg aagagaagcg tttcgagcag
    20341 tatttcctgc cttatcattc tctgtgcatg gtgcaaaatg cctcccgcga tgcggtgata
    20401 aaactccagc gtagtgagcg ggggattgag tgggggctgt atgccatctc tccgttgaac
    20461 ggataccgtc tggcgatccg cgaaatcggc aaatgcaacg cgttgctcga tgatgccgtg
    20521 gccctgacac cagcgaccgc catccagggc gtgttgcacg gtatcaatcc tgacagactg
    20581 accattgaac tctccgatgc cgacggcaat attgtactga gttatcagga acatcagccg
    20641 caggagttgc cgttgccgga cgtcgccaaa gcgccactgg cagcacaaga cattaccagt
    20701 acagatgaag cctggtttat cggtcagcat ctggagcaat atcatcacgc cagccgttca
    20761 ccgttcgatt actacctgcg cggcgtggcg ctggacccgc tggattatcg ctgtaacctg
    20821 gcgctggcga tgctggaata taaccgcgca gatttcccgc aagcggtggc gtatgccagt
    20881 caggacgcgc acatgcgctg aacaaaaacc cgcagtgtgg acaagcaagt ttgattcgcg
    20941 ccagtgctta cgaacgtcag ggacaatatc aacaagccga agaggatttc tggcgtgcgg
    21001 tctggagcgg caatagtaaa gcaggcggct attatggtct ggcacgactg gcggcgcgta
    21061 atgataactt cgacgcgggt ctggattttt gccaacaaag tcttcgtgcc tgcccaacca
    21121 atcaggaagt gctttgcttg cataatctgc tgctggtgtt aagtggtcgt caggacaacg
    21181 cgcgtttgca gcgcgagaaa ctgctgcgtg attatccgct gaacgccact ctgtggtggc
    21241 tgaactggtt cgatggtcgt agcgaatcag ccctcgcgca gtggcgcggt ctgtgtcagg
    21301 gacgcgacgt taacgctctg atgaccgccg ggcaactgat taactgggga atgcccactc
    21361 tggcggcaga gatgctgaac gcactggact gccagcgcac gctgccgctt tacctgcaag
    21421 ccagcttgct gccgaaagcc gaacgtggcg aactggtcgc aaaagccatt gatgtcttcc
    21481 cgcagtttgt ccgtttcccg aatacgctgg aagaagtggc ggcgctggag agtattgaag
    21541 agtgctggtt tgctcgccat ttactggcct gcttctacta caacaaacgt agctacaaca
    21601 aagccattgc cttatggcaa cgttgcgtag agatgtcgcc ggagtttgcc gacggctggc
    21661 gcgggttagc gatccatgcg tggaataaga aacacgatta tgagctggcc gcgcgttatc
    21721 ttgataatgc ttatcagctt gcgccgcagg atgcacgtct gcttttcgaa cgggatttgc
    21781 tggataagtt aagtggagcc acaccggaga aacgactggc gcgtctggaa aataatctgg
    21841 aaattgcgct gaaacgcgac gacatgaccg cagaactgct caatttgtgg catctcacgg
    21901 gtcaggcaga caaagcggcg gacattctcg ccacgcgtaa attccacccg tgggaaggcg
    21961 gggaagggaa ggtcaccagt cagtttatcc tcaaccagtt attacgcgcc tggcagcatc
    22021 ttgatgccag agagccgcag caggccagcg aactgcttca tgccgcgctg cattatccgg
    22081 agaatttaag cgaaggccgt ttaccggggc aaactgataa cgacatctgg ttctggcagg
    22141 cgatatgcgc caacgcgcag ggcgatgaaa ctgaagcgat gcgttgttta cgtctggcgg
    22201 cgaccggcga tcgcaccatt aacatccaca gttattacaa cgatcagccg gttgattatc
    22261 tcttctggca aggaatggcg ctgcgactgc tgggtgaaca gcaaaccgca cagcaactgt
    22321 ttagtgaaat gaaacagtgg gcgcaagaga tggcgaaaac cagtattgag gcggatttct
    22381 ttgctgtttc acaacctgac ctgttatcgc tgtatggcga tttacaacag cagcataaag
    22441 aaaaatgcct gatggtggcg atgctggcgt ccgcgggact cggggaggtt gcgcaatatg
    22501 agtctgctcg cgctgaattg acggcgatta atccggcctg gccgaaagcg gcattattca
    22561 ccactgtgat gccttttatt tttaactac
//
LOCUS       DAJTYF010000058        22466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTYF010000058 DAJTYF010000000
VERSION     DAJTYF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.058"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(214..2247)
                     /gene="metG"
                     /locus_tag="OO590_002333"
     CDS             complement(214..2247)
                     /gene="metG"
                     /locus_tag="OO590_002333"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6637678.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2379..3488
                     /gene="apbC"
                     /locus_tag="OO590_002334"
     CDS             2379..3488
                     /gene="apbC"
                     /locus_tag="OO590_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6637679.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3750..4031
                     /locus_tag="OO590_002335"
     CDS             3750..4031
                     /locus_tag="OO590_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6637680.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4324..4866
                     /locus_tag="OO590_002336"
     CDS             4324..4866
                     /locus_tag="OO590_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6637681.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            4947..5621
                     /locus_tag="OO590_002337"
     CDS             4947..5621
                     /locus_tag="OO590_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6637682.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5637..8117
                     /locus_tag="OO590_002338"
     CDS             5637..8117
                     /locus_tag="OO590_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6637683.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8133..9166
                     /locus_tag="OO590_002339"
                     /pseudo
     CDS             8133..9166
                     /locus_tag="OO590_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9248..9586)
                     /gene="rcnB"
                     /locus_tag="OO590_002340"
     CDS             complement(9248..9586)
                     /gene="rcnB"
                     /locus_tag="OO590_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6637684.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9805..10647)
                     /gene="rcnA"
                     /locus_tag="OO590_002341"
     CDS             complement(9805..10647)
                     /gene="rcnA"
                     /locus_tag="OO590_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR6637685.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHEHHQDHEHHHDQGHHHHHEHGEYQDAHA
                     RAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVS
                     FSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFM
                     GIMR"
     gene            10768..11040
                     /gene="rcnR"
                     /locus_tag="OO590_002342"
     CDS             10768..11040
                     /gene="rcnR"
                     /locus_tag="OO590_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR6637686.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11132..11228
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11263..12051
                     /gene="thiM"
                     /locus_tag="OO590_002343"
     CDS             11263..12051
                     /gene="thiM"
                     /locus_tag="OO590_002343"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6637687.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12048..12848
                     /gene="thiD"
                     /locus_tag="OO590_002344"
     CDS             12048..12848
                     /gene="thiD"
                     /locus_tag="OO590_002344"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6637688.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            12913..13731
                     /locus_tag="OO590_002345"
     CDS             12913..13731
                     /locus_tag="OO590_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6637689.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            13783..14529
                     /locus_tag="OO590_002346"
     CDS             13783..14529
                     /locus_tag="OO590_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6637690.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14503..15468)
                     /locus_tag="OO590_002347"
     CDS             complement(14503..15468)
                     /locus_tag="OO590_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6637691.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15465..16468)
                     /locus_tag="OO590_002348"
                     /pseudo
     CDS             complement(15465..16468)
                     /locus_tag="OO590_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16465..16941)
                     /locus_tag="OO590_002349"
                     /pseudo
     CDS             complement(16465..16941)
                     /locus_tag="OO590_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            17095..18147
                     /gene="fbaB"
                     /locus_tag="OO590_002350"
     CDS             17095..18147
                     /gene="fbaB"
                     /locus_tag="OO590_002350"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6637692.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18455..19309
                     /gene="gatY"
                     /locus_tag="OO590_002351"
     CDS             18455..19309
                     /gene="gatY"
                     /locus_tag="OO590_002351"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6637693.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            19338..20605
                     /gene="gatZ"
                     /locus_tag="OO590_002352"
                     /pseudo
     CDS             19338..20605
                     /gene="gatZ"
                     /locus_tag="OO590_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            20615..21067
                     /gene="gatA"
                     /locus_tag="OO590_002353"
     CDS             20615..21067
                     /gene="gatA"
                     /locus_tag="OO590_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6637694.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            21098..21382
                     /gene="gatB"
                     /locus_tag="OO590_002354"
     CDS             21098..21382
                     /gene="gatB"
                     /locus_tag="OO590_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6637695.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            21386..22466
                     /locus_tag="OO590_002355"
                     /pseudo
     CDS             21386..22466
                     /locus_tag="OO590_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
BASE COUNT         5841 a         5201 c         5687 g         5737 t
ORIGIN      
        1 gcaactcgtt tccctgttgc tccaccgtcc agaatttatg tgatttttcg tcctgataga
       61 taaagtgtct cattctgcat ccctgtaatt ttatccagtt gaatgcagat gctaccagta
      121 tttatacggg ttagagagag acaaaatcgc aagaaaacaa ctttaggaat ttataaagaa
      181 aaggcgctgt cgatgcagcg ccttgaaggg ggattatttc acctgatgac ccggtttagc
      241 accggcatcc gggcttagca ggaaaatatc tttcccgcca ggaccggcag ccatcaccat
      301 gccttcagag ataccgaagc gcattttacg tggtgccagg ttagccacca taatggtgtg
      361 acgaccaatc agtgcctgcg gatccgggta agcagaacga ataccggaga agacattgcg
      421 tttttcaccg ccgagatcca gcgtcaggcg cagcagtttg tcagaacctt caacaaactc
      481 tgcgttttcg atcagcgcca cgcgcaggtc aactttggcg aagtcgtcaa aggtgatggt
      541 ttcctgaatc ggatcatctg ccagtgggcc agtaactggc gcggtagtgg ctttcacttc
      601 ttctttagag gcttccacca gagcttcaac ctgtttcata tcgatgcggt tatacagcgc
      661 cttgaacgga ttcactttgt ggcccagcag cggttgctgg ataccatccc aggtcagttc
      721 cgtattgagg aaggcttctg cacgctcggt cagtttcggc agtaccggct tcaggtaagt
      781 catcagcacg cggaacaggt tgatgcccat tgagcaaatc gcctgcaggt cagcatcgcg
      841 accttcctgt tttgcgacta cccacggagc ctgctcgtcg acatagcggt tagccaggtc
      901 agccagtgcc atgatttcgc gcactgcttt accaaattca cggctttccc acgcttcacc
      961 aatcacttca gcggcatcgg taaaggtttt gtacagctca gggtcagcca gctcgcttgc
     1021 cagcacaccg tcaaaacgct tgttgataaa gcccgcatta cgggaggcca ggttaaccac
     1081 tttgttcacg atatcggcat tcacacgctg aacgaaatct tccaggttga gatcgatatc
     1141 atcaatgcgc gaagagagtt tcgcagtgta gtagtaacgc aggctgtcag cgtcaaaatg
     1201 attcagccag gtgctggctt taataaaggt gccgcgagac ttggacatct tcgcgccgtt
     1261 caccgtcaca tagccatgaa caaacaggtt ggtcggcttg cggaagttgc tgccttccag
     1321 catggcaggc cagaacaggc tgtggaagta aacgatatct ttaccgatga agtggtacag
     1381 ctcggcggtg gagtctttct tccagtattc atcgaagctt acgctgtcgc cgcgcttgtc
     1441 gcacagattc ttgaaagaac ccatgtagcc aatcggtgcg tccagccaga cgtagaaata
     1501 tttgcccggc gcgttcggaa tttcaaaacc aaagtatggc gcgtcgcggg agatatccca
     1561 ctgttgcagg ccggattcaa accactcctg cattttgttt gccacctgct cctgcaacgc
     1621 accgctgcgg gtccatgcct gcagcatttc gctgaaagag ggcagatcaa agaagaagtg
     1681 ttcagaatca cgcatcactg gtgtagcacc agaaaccacc gatttcggct cgatcaattc
     1741 agtcgggctg taggtcgcgc cgcagacttc gcagttatcg ccgtattgat ccggggattt
     1801 acatttcggg caggtgcctt tcacaaaacg gtccggcagg aacatgcctt tttccggatc
     1861 gtacagctga gagatggtgc ggtttttaat aaaaccgttt tctttcaggc gagagtagat
     1921 aagttctgac aactggcggt tctcttcgct gtgcgtcgag tgatagttgt catagctgat
     1981 gttaaagcct gcgaaatcag tctgatgctc ctgactcatt tcgccaatca tctgctccgg
     2041 ggtgataccg agctgctgag ctttcagcat gatcggtgta ccgtgggcat cgtcggcgca
     2101 gatgaagttg acctcgtggc cgcgcattcg ctggtaacgg acccagacat cagcctggat
     2161 gtgctccagc atatggccga ggtggattga gccgttagcg tacggcagtg cgcacgtcac
     2221 cagaattttc ttcgcgactt gagtcatagt aggcattact tcttaatagt gaaaaggggt
     2281 tagatagtac caaatgggaa aatgttaagt aagtacttct ctatattctt caggtaaact
     2341 atcagctgat ggtttatcta tacaaaaaag gagtcgggat gaacgaacaa tcccaggcca
     2401 aatcaccaga agccttgcgc gcaatggtcg ccgggacact ggccaatttt cagcacccaa
     2461 ccctgaagca taacctcacc acgctgaaag cgttgcacca tgtcgcctgg atggacgaca
     2521 cgttgcatgt ggaactggtt atgccgtttg tctggcatag cgcgttcgaa gagttaaaag
     2581 agcaatgtag cgccgaactg ctgcgcatca ccggggcgaa agctatcgac tggaaactgt
     2641 cgcataacat agccactctg aaacgcgtga agaatcagcc gggaattaac ggtgtgaaga
     2701 acattatcgc ggtgagttcc ggtaaaggcg gcgtgggtaa atcgtccacg gcggtaaacc
     2761 tggcgctggc actggcggcg gaaggggcga aagtcggcat tctggacgcc gatatctacg
     2821 gcccatcaat tcccaccatg ctgggcgcag aaaatcagcg tccaacttcg ccggacggta
     2881 cccacatggc accgattatg tctcatggcc tggcaaccaa ttcaattggt tatctggtca
     2941 ctgacgacaa tgcgatggtg tggcgtggac cgatggcgag caaggcgtta atgcagatgt
     3001 tgcaggaaac cttgtggccg gatctcgact atctggtgct tgatatgccg ccaggcaccg
     3061 gtgacattca attgacgctg gcgcaaaaca ttccagtaac tggtgcagtc gtggtcacca
     3121 ccccgcagga catcgcgttg atcgatgcga aaaaaggcat tgtgatgttc gagaaagtcg
     3181 aggttccggt gctcggtatt gtcgagaaca tgagtgtgca tatttgcagt aactgtggtc
     3241 accatgaacc gattttcggt accggtggtg cagagaaact ggctgaaaaa taccatacac
     3301 agctgctggg gcagatgccg ttacacatct ccttacgtga ggatctcgat aaaggcacgc
     3361 caacggttat cagccgtccg gagagcgaat ttaccgctat ctaccgccag ctggctgacc
     3421 gcgttgcagc tcagctctac tggcagggtg aagtcattcc tggcgagatt tccttccgcg
     3481 cggtctaacg cctattaacc attctggtta ttcttcatac atcgcagaga tgttatattg
     3541 atgcggtgta tttgtttttg ccgcattaat gaacacttat ttaatactgc gaatattctg
     3601 cggaccttcg tccgaaactc tacaaagtcg agtgatcgta caggcaatgc ttcattatca
     3661 tccttcgtgc tgtgtagtgg atgtacagct atagtttgtt atgcactcat tctatccagg
     3721 cataaccttt actacaggga taacgttcaa tgaataagta ttggctgtca ggaattattt
     3781 ttcttgccta tggattggct tctcccgcag tctcttcaga gactgccaca ttaacgatta
     3841 atggcaggat cagtccccca acatgcagta tggctatggt taatagtcaa ccacaacagc
     3901 attgtggtca gttaacgtat aatgttgaca cccgtcatca ggtttcttcg cccgtcaaag
     3961 gtgttacgac agaagtggtt gttgcaggta gcgatagcaa acgtcgaatc gtgttaaatc
     4021 gctacgatta atttggcaac ctgattttaa ttgcatttag aaagacgaaa tcatttatat
     4081 aaatgatttc gtctttttta ttgatatcaa caataccatt tacataaatc tacattaatt
     4141 aatagatgtg ataaatattt aatattctta agtatttgtt gtatttatat tgcattggtt
     4201 aatttgtttt aaatcaataa aacaatatgt ttacattgcg gcagcttttt tgatgtgaga
     4261 aatttgtttc caagagatac agactcttaa caaccaaaaa taaattttat taaggaacat
     4321 attatgaaac gttcaattat tgctgccgct gtcttttctt ctttttttat gagcgctgga
     4381 gtatttgctg cagacgttga taccggaaca ttaaccatta aaggaaatat tgcagaatct
     4441 ccgtgtaaat tcgaagcggg tggtgattca gtaagtatta atatgccgac tgtaccaacc
     4501 actgtctttg aaggtaaagc taaatattct acctatgatg atgcagtcgg tgtaaccagc
     4561 agcatgttaa aaattagctg cccgaaagaa gttgctggtg taaaactctc gttgatcaca
     4621 aacgacaaaa taaccggtaa cgataaggcg atagccagta gcaacgatac cgtgggttac
     4681 tatctctatt taggtgataa cagcgatgtc ctagatgttt ctgcaccttt taacattgag
     4741 agttataaaa cagcagaagg tcaatatgct attccgttta aagcaaaata cctgaaactg
     4801 acagataact cagtgcaatc aggtgatgtg ttatcttctc tggttatgcg tgtagcgcag
     4861 gattaataaa taaaattgca agtttctcca ggtaataaac atggccgcta tccccatggc
     4921 ggctttttaa tttaagaggc attaagatga aaggattatt atctttactc attttttcta
     4981 tggtccttcc tgcacatgcc ggaattgtta tctacgggac gcgcattatt tacccggcag
     5041 aaaataaaga agttatggtg cagttgatga accagggaaa tcgttcttcg ctgctgcagg
     5101 cgtggattga tgatggcgat acgtcgttac caccagaaaa aattcaggtt cctttcatgt
     5161 taacgccacc agtggcaaaa ataggggcaa attcagggca gcaagtaaaa atcaaaatta
     5221 tgccgaataa actgcccact aataaagaaa gcatttttta tctgaatgtt ctggatattc
     5281 caccaaatag tccagagcaa gaaggcaaga atgcactgaa gtttgcgatg caaaacagaa
     5341 ttaagttgtt ttaccggcca gcgggtattg ctccggtaaa taaagcgacc tttaaaaagt
     5401 tactggtaaa tcgcagtggc aatggattgg tgataaaaaa tgactcagct aattgggtga
     5461 cgatttcgga tgtcaaagct aataatgtta aagtcaatta tgaaactatt atgattgccc
     5521 ccttagaaag tcagagtgtt aatatcaaaa gtaataatgc aaataactgg tatctgacca
     5581 ttatcaatga ccacggcaac tatattagtg acaaaattta attgcaggag ctgcctatgt
     5641 tgagaatgac cccacttgca tcagcaatcg tagcgttatt gctcggcatt gaagcttatg
     5701 cagctgaaga aacctttgat acccatttta tgataggtgg aatgaaagac cagcaggttg
     5761 caaatattcg tcttgatgat aatcaaccct taccggggca gtatgacatc gatatttatg
     5821 tcaataagca atggcgcggg aaatatgaga ttattgttaa agacaacccg caagaaacat
     5881 gtttatcaag agaagttatc aagcggttag gcattaatag cgataacttc gccagcggta
     5941 agcaatgttt aacatttgag caacttgttc agggtgggag ctatagctgg gatatcgggg
     6001 tttttcgtct cgatttcagt gtcccgcagg cttgggtgga agaactggaa agtggctatg
     6061 ttccaccgga aaactgggag cggggtatta atgcgtttta tacttcttat tatgtgagtc
     6121 agtattacag cgactataaa gcgtcgggta acaacaagag tacatatgta cgttttaaca
     6181 gcgggttaaa tttactggag tggcaactgc attctgatgc cagtttcagt aaaacaaata
     6241 acaatccagg ggtgtggaaa agcaataccc tgtatctgga acgtggattt gcccaatttc
     6301 tcggcacgct tcgcgtgggt gatatgtaca catcaagcga tatttttgat tctgttcgct
     6361 tcagcggtgt gcggttgttt cgtgatatgc agatgttgcc taactcgaaa caaaatttta
     6421 cgccacgggt gcaggggatt gctcagagta acgcgttggt aactattgaa cagaatggtt
     6481 ttgtggttta tcagaaagag gttcctcctg gcccgtttgc gattacagat ttgcagttgg
     6541 ccggtggtgg agcagatctt gatgttagcg tgaaagaggc ggacggctcg gtaaccacct
     6601 atctggtgcc ttatgcagcg gtgccaaata tgctgcaacc cggcgtgtcg aaatatgatt
     6661 ttgcggcggg ccgtagccat attgaagggg cgagcaagca aagtgatttt gtccaggcag
     6721 gttatcagta tggttttaat aacttattga cgctgtatgg cggcacgatg gttgctaata
     6781 attactatgc gtttaccctc ggaacgggtt ggaacacgcg cattggcgca atttccgtcg
     6841 atgccacgaa gtcgcatagt aaacaagaca acggcgatgt gtttgacggg caaagttatc
     6901 aaattgccta caacaaattt gtgagccaaa cgtcgacgcg ttttggtctg gcggcctggc
     6961 gttattcgtc gcgtgattac cggacattta acgatcacgt ttgggcaaac aataaagata
     7021 attatcgccg tgatgaaaac gatatctatg acattgccga ttattaccag aacgattttg
     7081 gccgtaaaaa tagcttttct gccaatatga gccagtcatt gccagaaggc tggggttctg
     7141 tgtccttaag tacgttatgg cgagattact gggggcgtag cggcagcagt aaggattatc
     7201 agttgagtta ttccaataac tggcggcgga taagctatac cctcgcggca agccaggcgt
     7261 atggcgagaa tcatcatgaa gagaaacgtt ttaatatttt tatatcaatt ccctgtgatt
     7321 ggggtgatga cgttacgacg cctcgtcggc aaatatatat gtctaactca acgacgtttg
     7381 atgatcaggg ttttgcctca aataatacgg gattatcagg aacagtaggg agtcgggatc
     7441 agttcaatta tggtgtcaac ctgagtcatc aacatcaggg aaatgaaacg acagctgggg
     7501 cgaatttgac ctggaacgcg ccggttgcga cagtgaatgg cagttatagt cagtcgagta
     7561 cttatcgaca gactggagcc agtgtttcag ggggcattgt cgcctggtcg ggtggcgtta
     7621 atctggcgaa ccgtctttcc gaaacgtttg ctgtgatgaa tgcgccagga attaaagatg
     7681 cttatgtcaa tgggcaaaaa tatcgcacaa caaaccgtaa tggagtggtg gtatacgacg
     7741 gaatgacacc ttatcgggaa aatcacctga tgctggatgt gtcgcaaagc gatagcgaag
     7801 cagaattacg tggcaaccgg aaaattgccg ccccttatcg cggcgcggtt gtactggtta
     7861 attttgatac cgatcagcgc aagccctggt ttataaaagc gttaagagcg gatggtcaac
     7921 cattaacgtt tggttatgaa gtcaatgata tccatggtca taatattggc gttgtcggcc
     7981 agggaagcca gttatttatt cgcaccaatg aaataccgcc atcggttaat gtagcaattg
     8041 ataagcaaca aggactttca tgcacaatca ccttcggtaa agagattgat gaaagtagaa
     8101 attatatttg ccagtaatag tcaggtggtt ctatggaaat tcgcataatg ctatttatat
     8161 taatgatgat ggttatgcct gtgagctatg cggcatgtta tagtgagtta tctgttcagc
     8221 acaatttggt tgttcagggg aattttgcac ttactcaaac acaaatagcg acatatgagc
     8281 ataattttaa tgattcgtca tgcgtgagta caaatactat cacccctatg agcccatcgg
     8341 atattattgt tggactttat aacgatacca ttaaattaaa tttacatttt gaatggacca
     8401 ataaaaacaa catcacgttg tcaaataatc agagcagttt caccagtggt tattcggtaa
     8461 cggtgacacc tgcggccagt aatgcaaaag tgaatatttc tgcggggggc ggtggttcag
     8521 tgatgattaa tggtgttgcg acattatcca gtgcttcatc atcgacacgc gggagtgccg
     8581 cagtacaatt tctactgtgt ttattaggtg gcaagtcatg ggatgcatgt gtaaatagct
     8641 acagaaatgc attggcacaa aatgcaggtg tctattcctt caatctgaca ttgtcataca
     8701 acccgataac cacaacctgc aaacaggacg atttattaat tactttagac agtattcccg
     8761 tttcacaatt accagccaca ggtaacaaag caacaataaa tagtaaaaaa agggatatta
     8821 ttctgcgttg taaaaattta ttaggtcaac aaaatcaaac atcacggaaa atgcaggtgt
     8881 atttatcaag ttctgacttg ttaaccaaca gcgacacaat actgaaaggt gcggaagata
     8941 atgcgtagga tttattcttg aaagtaatgg ttcgccagtc acacttttaa atatcactaa
     9001 cagcagtaaa ggatacacaa atttaaaaga aattgcggca aagtcaaaac ttacagatac
     9061 aacggtttca attccgataa cagccagtta ctacgtctac gatacaaaca aagttaaatc
     9121 tggcgcactg gaggcaaccg cattaatcaa cgtgaaatac gactaataac aagcaagacg
     9181 agcaagtggc taataataaa aaataacaag gtgtctgcat ttccccctca tgatgagggg
     9241 gcttttttta gcgatgataa aaaatctcac cgtcgtaggc tttaatgatt ttaccgtcgg
     9301 tgtcgctgat caacacgtac gcgccaccca tataggtcca gtgcgtcccg gcatcaggcg
     9361 cgggcaggtt acgtaactgc cactgcttaa tgttgtactc atcggtgcgg tacatctctg
     9421 ggacggtatc acctggtttg aaatgcttaa agtcagcaat gaaactactt aactcatact
     9481 tagaaattcc cgaggtattg gtcgaacccg cggtggctgg tgcggcccag gcggcactgg
     9541 taaccagcaa aagcacaccc agcaacatct tatttttaat ggtcataaac tctccacttt
     9601 ggggatctgt aatatcagtc gcatatcttt cctgccatac aatataactg acaagcgata
     9661 ttttgttagt gatcccatga tttcgtaaaa agtgtaaatg aaaggatgcc ctaaaagcat
     9721 ccttagcgag cagagagtca gcggaaatag ctgatttaag aatcatcctg ggctgaagat
     9781 aacatgcaag cctaaaggat tgagttatcg cattatgccc atgaagccat gtacgcccat
     9841 atacacaccg actaagccaa tcaacagact ggaaaaatag ggggcgcgtt tagcgagagt
     9901 gttaaatccg ctccagcgtt ttgcgacctg ctgaacgctg attgctgcgc caacgcctac
     9961 ggtgacaagc gttaacgcca ggccaatgct gaaactgacg accagtgttg cgcccagtgt
    10021 cagggctttc aactgaatgc atatcaacag cacggtaatt gctgccgggc aggggataag
    10081 gccaccggtt aagccaaata acaaaatttg ccagttgatg acctctctac catcaaagcg
    10141 tcgtttaatg tcattggcat gggctcgtgc atgggcatcc tgatactcgc catgttcgtg
    10201 atggtgatga tgtccttggt cgtggtgatg ttcgtggtct tgatgatgtt cgtgatgatg
    10261 gtgatgatgc tcataatcat gcccgtgcat attctccagc cagttgcgtt cgccgcgcca
    10321 ggtacgccag aacatccaga acacggtgcc aataatgatc actgcggaaa tcagctggag
    10381 ccacggttct gctgattgag cagtaaagcg cttactgatc accatcccgc caaaggcaat
    10441 caaccagacc accgcggtat gcgaaatggt tgctgccagt ccgagcatca ccgcctgttt
    10501 aatggtgcct ttgatggcga tgataaacgc cgccatcatc gtttttgagt gccctggttc
    10561 caggccatga agcgcaccaa gtaagatggc gctggggatg aagaaccagg cgtttccttg
    10621 ctgaagaaga gttgtaaatt cggtcatgat aataattctt agtattaatt cggcaatctg
    10681 attctactcc cccccagtac ctgatactac cccccagtag attaatagtg ctatgatttt
    10741 tcatattctt gttaaccagg tgttgccatg tctcatacaa tccgtgataa acagaaactg
    10801 aaagcgcgtg ccagtaagat tcagggccag gtcgtggcgc tcaagaaaat gctcgacgag
    10861 ccgcacgaat gcgctgcagt tttacaacag attgctgcta tccgtggagc ggtaaacggt
    10921 ctgatgcggg aagtgattaa aggtcatctg acggaacaca tcgttcacca gggggatgag
    10981 ctaaaacgtg aagaagatct ggatgtcgtt ctgaaggtgc tggattcata tatcaaataa
    11041 tttattaacg cgattgtaaa actgccgttt ttcctcgttt acaacgggtg cgctggacat
    11101 taccatcctc ctctgcgatt tatcatcgca accaaacgac tcggggtgcc cttctgcgtg
    11161 aaggctgaga aatacccgta tcacctgatc tggataatgc cagcgtaggg aagtcacgga
    11221 ccaccaggtc attgcttctt cacgttatgg caggagcaaa ctatgcaagt cgacctgctg
    11281 agttcagcgc aatctgcgca cgcgttacac ctttttcacc aacattcccc tcttgtgcac
    11341 tgcatgacca atgatgtggt gcaaaccttt accgccaata ccttgctggc gctcggtgca
    11401 tcgccagcga tggttatcga aaccgaagag gccagtcagt ttgcggctat cgccagtgcc
    11461 ttgttgatta acgttggcac actgacgcag ccacgcgcac aggcgatgcg tgctgccgtt
    11521 gagcaagcaa aaagctctca aacacgctgg acgcttgatc cagtagcggt tggtgcgctc
    11581 gattatcgcc gccatttttg tcatgaactt ttatctttta aaccggcagc gatacgtggt
    11641 aatgcttcgg aaatcatggc attagctggc attgctaatg gcggacgggg agtggatacc
    11701 actgacgccg cagctaacgc gatacccgct gcacaaacac tggcacggga aactggcgca
    11761 atcgtcgtgg tcactggcga ggtggattat gttaccgatg gacatcgtat cattggtatt
    11821 cacggtggcg atccgttaat gaccaaagtg gtaggaactg gctgtgcatt atcggcggtt
    11881 gtcgctgcct gctgtgcgtt accaggcgat acgctggaaa atgtcgcatc tgcctgtcac
    11941 tggatgaaac aagccggaga acgcgcagtc gccagaagcg aggggccagg cagttttgtt
    12001 ccacatttcc ttgatgcgct ctggcaattg acgcaggagg tgcaggcatg aaacgaatta
    12061 acgctctgac gattgccggt actgatccga gtggtggtgc ggggattcag gccgatctta
    12121 aaaccttctc ggcacttggc gcttatggtt gctcagttat tactgcactg gtggcgcaaa
    12181 atacccgtgg cgtacagtcg gtgtatcgca ttgagcctga ttttgtcgcc gcccagctcg
    12241 attcggtgtt cagcgatgtg cgaatcgata ccactaaaat cggtatgttg gcggaaaccg
    12301 atattgttga agcggtggca gaacggttgc aacgttatca gatccaaaac gtggtactcg
    12361 acaccgttat gctggcaaaa agcggcgacc cgctgctttc accttcggcg gttgctacgc
    12421 tgcgcagtcg attattgcca caggtttcat taataacgcc aaacttgccc gaagctgccg
    12481 ccttgctcga cgcgccacac gcgcgcaccg aacaggaaat gctggaacaa gggcgatcgc
    12541 tgttggcgat gggctgtggc gcagtgctaa tgaaaggtgg tcatctggat gatgagcaaa
    12601 gcccggactg gctgtttacc cgcgagggtg aacaacggtt taccgcaccg cgcattatga
    12661 ccaaaaacac ccacggcact ggttgtacac tctctgcggc gttggctgca ctacgcccgc
    12721 gccatacaaa ctgggctgac accgtacagg aggcaaaaag ctggctttca tcggcgttag
    12781 cccaggccga cacgctggaa gttggtcacg gtattggtcc ggttcaccac ttccacgcct
    12841 ggtggtgagc accgtactat agctgctttg gttattgtca gcctttggca ctgacattca
    12901 gggatgaaac caatgcagct aagaattacc agtcgaaaaa aactcaccgc tctattatgc
    12961 gctcttgggc taatttccat cgttgcaatt tatccgcgtc agacggtgaa ttttttctat
    13021 tcgacagcag ttcagattac agactacatc cacttctacg gttatcgccc ggttaaatct
    13081 ttcgctattc gcattcctgc cagttacacc attcacggat tagatgtttc gcgctggcag
    13141 gagcgtatcg actggcagcg ggtggcaaaa atgcgcgaca acgatatccg cttacagttt
    13201 gcttttatta aggctacgga aggcgaaaag ctggtggacc cctatttttc gcgtaactgg
    13261 caattaagcc gcgaaaatgg cctgctgcgc ggggcgtatc attatttctc cccgtcggta
    13321 tccgcttcag ttcaggcgcg attatttctg caaacggtgg atttctcaca aggcgatttc
    13381 cctgccgtgc tggacgtaga agaacgggga aaattatcgg caaaagaatt acgcaagcgg
    13441 gtaagtcagt ggctaaaaat ggtcgaaaaa cgcacgggaa aaaagccgat tatttactca
    13501 ggagccgttt tttatcacac caatctggcg ggctatttca atgaatatcc gtggtgggtg
    13561 gctcactatt atcaacgtcg tccggacaat gacggcatgg cctggcgctt ctggcagcat
    13621 tccgaccgtg gacaggtaga tggcatcaat ggtccggtgg attttaatgt gtttaatggc
    13681 acggtggatg agttgcaggc attcgttgat gggattaaag aaacgcctta aataagaagc
    13741 agtgatatac tcccaccaga ctccctggca ggaaaaagac aaatggaaca agcgcatacc
    13801 cagctgattg cccaactgaa cgagcgtatt ttagcggcgg ataacacgcc gctttatatt
    13861 aagtttgccg aaacggtaaa aaatgccgtg cgcagcgggg tgctggagca tggcaatatt
    13921 ttgcccggtg agcgtgattt aagtcagtta accggcgttt cgcgcattac ggtgcgcaag
    13981 gcgatgcagg cgctggaaga agagggcgtg gtgacgcgtt cgcgcggtta cggcacgcag
    14041 atcaacaaca tcttcgaata ctcgttgaaa gaagcgagag gtttttctca gcaggtggta
    14101 ttgcgcggga aaaagcccga tacgctatgg gttaataagc gtgtcgtgaa atgtcccgaa
    14161 gaagttgcgc agcagctggc ggtcgaagcg ggaagtgatg tctttttgct taagcgtatt
    14221 cgctatgtcg atgaagaagc ggtatcgatt gaggaatcgt gggttccggc gcatttaatt
    14281 catgatgttg atgccatcgg aatttctctt tatgactatt tccgcagcca gcacatttac
    14341 ccacaacgta cgcgttcccg cgttagcgcc cggatgccgg atgccgagtt tcagtcacat
    14401 attcagttag atagcaaaat accggtgctg gtgatcaagc aagtggcgct tgaccaacag
    14461 caacggccta ttgagtacag cattagccac tgtcgcagcg atctatacgt ttttgtgtgc
    14521 gaggagtagt tcctcgcgcg ttggcgcaca atcaccgccc cgatgcccga caacccacga
    14581 cgccactgca ttgcccagta atacggcatc cgccagtggc agaccagagg ccagcccggc
    14641 aagtacgcca cctgcatgac tgtcgcccgc cccaatggtg tctacaactt gcgttggaaa
    14701 tgcccgaatg cagccagaag cgtcgttgct gaaataccat gcgccttctt tatcgagacg
    14761 aacgatcaac ggcgcggcaa atttctcctg ccattgcttg ccaagtgttg ttatctctgc
    14821 agataaagca aaacgttcgg cggcaatctc agcctcttga cgattgagtg acactaaagg
    14881 tcgacaggcc atgatccgcg ccagtaatgc gtccgggata tcgccaatac gtgggccaaa
    14941 atcgataaac ggcgtcacgt cttgcagctt ttccagccat tccactaaca attcgccgca
    15001 gggcgaggcc agttgataac cggaaaaata gagcaggttg ccaggcgcaa cggttaatcg
    15061 tgccaaccat tggcgattcc attgattttc cacaccgctg aatgacataa aagtgcgttc
    15121 gccatccggc tcaaccagtg ccagacacca gccgttatcg ctttcggcgt tatcgatcag
    15181 gctgattaac ccctctttcg ccatccggtt gcgaataatc tccgcccaca cgccctgacc
    15241 gagcggcaag gcattacccg cttcgatgcc gaggcgcttt aacgccacgg caatattcag
    15301 tgcgcagccg ccaacgttaa cgctctgctg tttcagttcg atatcgcacc cacgccaggg
    15361 gagggcataa gcgtcggcga tcacgtcaat gaccgccgcg ccgacgacca tcaccggctg
    15421 acgcgtggtt aattcaggca gcagtgtgtg taatcgagcg ccgctcatac cgcctcccgt
    15481 tgttgacgat atttctccag cgctgtggca tagcggttga aatcaagctg atttaccgca
    15541 tccagctccg cctttaacgc aggatcgaca gcgttaatgc catgcaacgc gccgcaaatc
    15601 gccgtcgcca tagcacctat ggtgtctgtg tcgccgccaa gattggcgca caggacggcg
    15661 cagcggtttg ggtcggtttg tgccagttca actagcgcaa tggcgcacgg aacggactca
    15721 atagtgctgg tgcctgcgcc aacgacctgg taaagctgtt cgctggcgga ttcggtgccg
    15781 tcggcattac gcacaatttt cagcgcaatc tccagacgtg ccgccagtga ggcgctgaac
    15841 gtggtggtgc gtttttgctg tgcatgtcgc gcaattgaag gcaatgaatc aacaatcgct
    15901 gaccagcgtt ctccgtcaat ggcacgagaa atcgcccatg cgatgactac cgcgcctgca
    15961 accgccagat cggatttatg ggtcgggctg gacgccagcg ccacatcatc aataaaggaa
    16021 tcaacatcac gcgctggaag caaacaacct aatggcgaga cgcgcatcgc tgcgccgttg
    16081 gttacgccgt tgttttccag ttcagcaacg ggtttaccgt cgcgaatggc gttaagcgca
    16141 atcttcgagg tcggacctag tacgtttttg ttaaaggcgt cgaaacgcaa cgcccagtcg
    16201 agaatattac gcccaatcag attcggatcg atcttgcctt cacattccag taacgcatcc
    16261 gccagacaca ggccatcgag gtatcgtcgg tgaattcggc gcggttaaaa taacaggccg
    16321 cgttattctc ctttggtcca ggcagaaaac ggtcaatcca gccaaagtgt gctttaacgc
    16381 ggctgcgtgg ccaaagctcg gagggcatcc ccatcgcatc ccctaacgcc tgcccataaa
    16441 gagcaccgag aatacgttct gttttcattt aacttcccct tgtgtcaacg caatatctcg
    16501 atcatcgacc ttgatagccg taatttcgtt gtcggattcg cgggaaaaaa tcatgaacag
    16561 cacggcgata atggcaatca tcaccgcgcc gaaagtccac atcccggacc agttgaaagt
    16621 cagtccgttt accggttcct gataagcgaa catcttttcc gtcatcacac cgccaagacg
    16681 atagccgagc aaactgccga agccctggca gcagagcgtg atcagcccct gcgcagcggt
    16741 acgcatatgc acgggggctt ttttatcgac atagatgtaa gcggtaacgt agtaaaaatc
    16801 gtaacttacg ccgtgaagca aaataccgag gaacagtaac gcgtaggtga aattgacagg
    16861 taatgtaagg tacgcgatga caataccaca taataatacc ttttgaatac ctttgcggta
    16921 aaaacttgac cgcgctaaca ttttttctga tgaatcgagc caacagaaaa cgctgaaaaa
    16981 acatccaaaa gatggaaaaa ctcgcccgta acgaccagga ttatgattgc ccgcaaaagg
    17041 cgggccagga caatccatag ccgatatcca atcggaattt acgggagcat agtaatgaca
    17101 gatattgcgc agttgcttgg caaagacgcc gacaaccttt tacagcaccg ttgtatgact
    17161 attccttctg accagcttta tctccccgga catgactacg tagaccgcgt gatgattgac
    17221 aataatcgcc cgccagcggt gttacgtaat atgcagacgt tgtacaacac cgggcgtctg
    17281 gctggcacag gatatctttc tattctgccg gttgaccagg gcgttgagca ctctgccgga
    17341 gcttcatttg ctgctaaccc gctctacttt gacccgaaaa acattgttga actggcgatc
    17401 gaagcgggct gtaactgtgt ggcatcaact tacggcgtgt tggtgtcggt atcgcggcgc
    17461 tatgcgcatc gcattccatt tctcgtcaaa cttaatcaca acgagacgct aagttacccg
    17521 aatacctacg atcaaacgct gtatgccagc gtggagcagg ccttcaacat gggcgcggtg
    17581 gcggttggtg cgactatcta ttttggctcg gaagagtcac gtcgccagat tgaagaaatt
    17641 tctgcggcct ttgaacgcgc gcacgagctg ggcatggtgg cagtgctgtg ggcctatttg
    17701 cgaaactccg cctttaagaa agatggcgtt gattaccatg tttccgccga cctgactggt
    17761 caggcgaacc atctggcggc aaccataggt gcagatatcg tcaaacaaaa aatggcggaa
    17821 aataacggcg gctataaagc gattaattac ggttataccg acgatcgcgt gtacagcaag
    17881 ttaaccagcg aaaacccgat tgatctggtg cgttatcagt tagctaactg ctatatgggc
    17941 cgggctgggt tgataaactc cggcggtgct gcaggcggtg aaactgacct cagcgatgca
    18001 gtgcgtactg cggttatcaa caaacgcgca ggcggaatgg ggctgattct tggacgtaag
    18061 gcgttcaaga aatcgatggc tgacggcgtg aaactgatta atgccgtgca ggacgtttat
    18121 ctcgatagca aaattactat tgcctgatac gcctcatacg aacagcacca ccccgtagga
    18181 cggataaggc gtttacactg catccggcaa cctatgcctg atgcgacgcg gaagcgtctt
    18241 atcatgccta catagcactg ccacgtatgt ttacaccgca tccggcataa aaacacgcgc
    18301 actttgctac ggcttcccta tcgggaggcc gtttttttgc ctttcactcc tcgaacaatt
    18361 ttcatattgt cgtttttgtg atcgttatct cgacatttaa aagcaaataa tttcattatt
    18421 ttttgaaatc gaaagcaaac aacaagatat gaaaatgtac gtggtatcga caaagcagat
    18481 gctgaacaac gcacagcgcg gcggttatgc ggttccggca ttcaatattc acaatctcga
    18541 aacgatgcaa gtggtggtgg aaaccgctgc caacctgcat gcgccggtca tcatcgccgg
    18601 aacgcctggc acatttactc atgctggtac agaaaatctg ttggcgctgg tcagcgcgat
    18661 ggcgaagcaa tatcaccatc cactggcaat tcatctcgac catcacacga aatttgacga
    18721 tatcgctcag aaggttcgtt ctggcgtgcg ctcagtcatg attgacgcct cgcatttgcc
    18781 ttttgcgcaa aatatttcac gggtcaaaga ggtggtggat ttttgccatc gctttgatgt
    18841 cagcgtcgag gcggagctgg ggcaacttgg cggccaggaa gatgatgtgc aagtcaatga
    18901 agccgatgcg ttgtacacca accccgcaca ggcgcgtgaa tttgccgagg caaccgggat
    18961 tgattccctg gcggtcgcca tcggcacggc tcacggtatg tatgccagcg caccggtgct
    19021 tgatttttct cgactggaga acattcgcca gtgggtgaac ttaccgctgg tgctgcatgg
    19081 cgcgtcagga ttatcgacta aggatattca gcaaaccatc aaactgggga tatgcaaaat
    19141 caacgttgca acggagctga aaaatgcctt ctcgcagtcg ttaaaaaatt acctgaccga
    19201 gcaccccgaa gcgaccgatc cccgggatta tttgcagtcg gctaaatccg caatgcgcga
    19261 tgtggtgagc aaagtgattg ccgattgtgg ctgcgagggc agggcataac gcacctgcca
    19321 tttaacaagg aaaaaacatg aaaacgttaa ttgcccggca taaagctggt gaacatatcg
    19381 gcatatgttc agtctgttct gcccatccgt tggttatcga agcggcgctg gcatttgatc
    19441 gcaacagcac gcgcaaagtg ctgattgaag caacgtcaaa ccaggtcaat caatttggcg
    19501 gttataccgg aatgacactg gcagactttc gcgaatttgt ttttacgatt gccgataaag
    19561 ttgggtttgc acgcgaacgc attattctcg gcggcgatca tctggggcca aactgctggc
    19621 agcaagaaaa tgcggatgcg gcgatggaaa aatccgtcga gctggtaaaa gcatatgttc
    19681 gtgctggctt cagtaaaatt catcttgatg cgtcaatgtc ctgcgcgggg gatcccatac
    19741 cgttagcacc agaaacggtt gcggaacgag ctgctgtgct ttgctttgct ttgctgcgga
    19801 aagtgtggcg acagattgcc agcgtgagca actgagctat gtcattggca ctgaagttcc
    19861 ggttccgggc ggtgaggcca gcgccattca gtcagtacac atcacccatg ttgaagatgc
    19921 cgccaatact ttacgtacgc atcaaaaggc ctttattgcc cgtgggctga cagaggcgtt
    19981 aacacgtgtg attgccatcg tggtgcagcc gggtgtggaa tttgatcaca gcaatattat
    20041 ccattatcag ccgcaggaag cgcaggcgct ggcgcaatgg atagaaaaca cccgaatggt
    20101 ttatgaagca cattctaccg attaccagac ccggacggct tattgggaat tagtccgcga
    20161 tcactttgca atattgaaag tcggtcctgc attaaccttt gctttacgcg aggcgatatt
    20221 tgcactggca caaattgagc aggaacttat cgcccccgaa aatcgcagcg gttgcctggc
    20281 ggtaattgaa gaagtgatgc tcgacgaacc gcaatactgg aaaaaatatt atcgcacggg
    20341 ttttaacgat tcattactgg atattcgtta cagcctgtcg gatcgtattc gttattactg
    20401 gccgcatagt cggattaaaa atagcgttga aacgatgatg gtgaatctgc aaggcgtgga
    20461 catcccactg ggcatgatta gtcagtatct tcccaaacaa tttgaacgca ttcagtccgg
    20521 ggaattatca gcaatgccgc atcagcttat tatgaataaa atttatgatg ttttgcgcgc
    20581 ctatcgctac ggttgtgcgg aataaggacg gtatatgact aacctgtttg ttcgtagcgg
    20641 aatttctttt gtcgatcgta gcgaagtttt aacctatatc ggtaatgaga tgctcgcgaa
    20701 aggtgtggtt catgatacct ggccacaggc attaattgcc agagaagcag aattccctac
    20761 cgggataatg cttgagcagc acgccattgc aataccgcat tgtgaggcga ttcatgctaa
    20821 gtcgtcagcc atttatctgt taaggccaac aaataaagtt cattttcagc aagcggatga
    20881 tgataacgac gtggcggtat cgttggttat tgcgttgatt gtggaaaatc cgcagcagca
    20941 attgaaactt ttacgctgtt tatttggcaa gttacaacag cccgatatcg tcgagacact
    21001 aattactctt cctgaaactc agttaaagga atacttcaca aagtatgttt tagattcaga
    21061 cgaataaatc cctctgtaac aataaaaagg actatttatg aaacgcaaga ttattgtcgc
    21121 ttgcggaggc gcggttgcga cctctacgat ggcggcggaa gaaattaaag agttgtgtca
    21181 gagtcataat attcctgttg aattaatcca gtgtcgggtt aatgaaatag aaacctatat
    21241 ggatggtgtg catttgatat gtaccactgc cagggtggat cgtagttttg gcgatattcc
    21301 gttagttcac ggcatgcctt ttgtttctgg tgtcggtatc gaagcattac aaaataaaat
    21361 tctgactatc ttacaggggt gacctatgtt ttcagaagtc atgcgttata ttctcgacct
    21421 cggccctaca gtgatgctgc cggttgtcat cattattttt tctaaaatat taggcatgaa
    21481 ggcaggcgat tgctttaaag cgggtctgca tatcgggatt ggctttgttg gcattggcct
    21541 tgtgattggc ttaatgctgg attccattgg tccggcggcg aaagcgatgg cggaaaattt
    21601 cgacctgaat ctgcatgtgg tcgatgtcgg ctggccgggc tcttcaccaa tgacctggac
    21661 gtcgcaaatt gcgctggtgg cgattccgat tgcgatattg gttaacgtgg cgatgttact
    21721 gacccgtatg acgcgggtgg taaatgttga tatctggaat atctggcata tgaccttcac
    21781 cggcagcgtt gctgcacctg gcaaccggtt catggatgat agggatggcg ggtgtggtaa
    21841 ttcacgcggc gtttgtttat aagctcggcg actggtttgc ccgcgatacc cgaaatttct
    21901 ttgagctgga aggtattgct attccgcacg gtacgtcggc gtatatgggg ccgattgcgg
    21961 tgctggtcga tgctatcatc gagaaaatcc caggcgttaa ccgaattaaa tttagcgccg
    22021 acgatattca gcgcaaattt ggcccttttg gcgaacctgt caccgtgggt tttgtgatgg
    22081 ggctgattat cggcatcctc gcgggttacg atgtcaaagg tgtattgcag ctggcggtaa
    22141 aaacggcggc agttatgctg ctgatgccac ggatgattaa acccatcatg gatggtttaa
    22201 cgcccatcgc taagcaggcc cgtagtcgtt tacaggcgaa gttcggcggt caggagttcc
    22261 tgattggcct ggatcccgca ttactgctgg ggcatacggc ggtggtatcg gcaagcctga
    22321 tttttattcc gctcaccatt ttaattgctg tttgtgttcc gggtaatcag gtactgccgt
    22381 ttggcgatct tgccactatc ggcttcttcg tggcgatggc ggtcgcggtg catcgtggaa
    22441 atctgttccg caccttaatc tcgggt
//
LOCUS       DAJTYF010000059        22342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTYF010000059 DAJTYF010000000
VERSION     DAJTYF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.059"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..464)
                     /locus_tag="OO590_002356"
     CDS             complement(<1..464)
                     /locus_tag="OO590_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6637696.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQC"
     gene            complement(554..727)
                     /locus_tag="OO590_002357"
     CDS             complement(554..727)
                     /locus_tag="OO590_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637697.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(875..2849)
                     /gene="pdeR"
                     /locus_tag="OO590_002358"
                     /pseudo
     CDS             complement(875..2849)
                     /gene="pdeR"
                     /locus_tag="OO590_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            2903..3005
                     /locus_tag="OO590_002359"
                     /pseudo
     CDS             2903..3005
                     /locus_tag="OO590_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3083..5017)
                     /locus_tag="OO590_002360"
     CDS             complement(3083..5017)
                     /locus_tag="OO590_002360"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6637698.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(5085..6212)
                     /locus_tag="OO590_002361"
     CDS             complement(5085..6212)
                     /locus_tag="OO590_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6637699.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRENNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(6358..7146)
                     /gene="fabI"
                     /locus_tag="OO590_002362"
     CDS             complement(6358..7146)
                     /gene="fabI"
                     /locus_tag="OO590_002362"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6637700.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(7514..7867)
                     /locus_tag="OO590_002363"
     CDS             complement(7514..7867)
                     /locus_tag="OO590_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6637701.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(7935..8741)
                     /gene="sapF"
                     /locus_tag="OO590_002364"
     CDS             complement(7935..8741)
                     /gene="sapF"
                     /locus_tag="OO590_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6637702.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(8743..9735)
                     /gene="sapD"
                     /locus_tag="OO590_002365"
     CDS             complement(8743..9735)
                     /gene="sapD"
                     /locus_tag="OO590_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6637703.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(9735..10625)
                     /gene="sapC"
                     /locus_tag="OO590_002366"
     CDS             complement(9735..10625)
                     /gene="sapC"
                     /locus_tag="OO590_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6637704.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(10612..11577)
                     /gene="sapB"
                     /locus_tag="OO590_002367"
     CDS             complement(10612..11577)
                     /gene="sapB"
                     /locus_tag="OO590_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6637705.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(11574..13217)
                     /gene="sapA"
                     /locus_tag="OO590_002368"
     CDS             complement(11574..13217)
                     /gene="sapA"
                     /locus_tag="OO590_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6637706.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVDVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(13530..13775)
                     /locus_tag="OO590_002369"
     CDS             complement(13530..13775)
                     /locus_tag="OO590_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6637707.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(13909..15294)
                     /gene="puuP"
                     /locus_tag="OO590_002370"
     CDS             complement(13909..15294)
                     /gene="puuP"
                     /locus_tag="OO590_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6637708.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(15284..15448)
                     /locus_tag="OO590_002371"
     CDS             complement(15284..15448)
                     /locus_tag="OO590_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637709.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(15597..17015)
                     /gene="puuA"
                     /locus_tag="OO590_002372"
     CDS             complement(15597..17015)
                     /gene="puuA"
                     /locus_tag="OO590_002372"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6637710.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            17227..17991
                     /gene="puuD"
                     /locus_tag="OO590_002373"
     CDS             17227..17991
                     /gene="puuD"
                     /locus_tag="OO590_002373"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6637711.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            18018..18575
                     /gene="puuR"
                     /locus_tag="OO590_002374"
     CDS             18018..18575
                     /gene="puuR"
                     /locus_tag="OO590_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6637712.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNVN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            18725..20213
                     /gene="puuC"
                     /locus_tag="OO590_002375"
                     /pseudo
     CDS             18725..20213
                     /gene="puuC"
                     /locus_tag="OO590_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            20215..21495
                     /gene="puuB"
                     /locus_tag="OO590_002376"
     CDS             20215..21495
                     /gene="puuB"
                     /locus_tag="OO590_002376"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6637713.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            21533..>22342
                     /locus_tag="OO590_002377"
     CDS             21533..>22342
                     /locus_tag="OO590_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6637714.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHSDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLA"
BASE COUNT         5575 a         5847 c         5828 g         5092 t
ORIGIN      
        1 atgcactggc gtgtcaaagg gccaaccata ctttcgcttt ttttctggta cacgccaccg
       61 agcaaaatga cttcacaagg cgcgtctttc agcaaatgcg cgatgtagct gctgaccgtg
      121 atgatagtga catttttctt ctgctccccc agagtccggg cgagcagggc attgctgctg
      181 ccattttcga taaagatggt ttcgcccggt tgaaccagtg acgcggcaaa ctcggcgagt
      241 tcacgcttca gcgtatagtt gctcatcata cgggtttcga cgtcatcact atcaagcgaa
      301 actgcaaagc catgtgcacg gcggaggtaa ctcagttttt cgagggtgtt gagatcctgg
      361 cgaatggtaa cttcagaaac tccagtggct tttgccagat cggttacgct aacctgaccc
      421 tggtcaatga ccatctgtag aatagtttgt tgtcgggaat tcatcgctgt aattcttata
      481 acgttataat actaaataaa aaaatactga cgcgccgggc atcctcaaat aggaaaacct
      541 gaaggcacga ttgttaaatc tcccacggtg tcttcgggat gtctggcgta gattcagcaa
      601 cctggcgcaa gagttctgct ttaagtatct cgagattatg gatcgcagag tggtaatcgc
      661 ctgccacaag gatatctaac acaatatcaa tacgttcggc aacgcgctgg gcatcaaatt
      721 cagacatcaa ggacatcctt atagcaaaag ggggaaaatg aataatgcaa aaattgccgc
      781 taaaagagaa gtgtttaaca gcaacggcta attatcatcc aggaatacga tatataatga
      841 cgggatatag cgctaagtat atatattcat ctacttatgc gcgcttcaga tagcgtttat
      901 accagcgttc gaaggcgacg gcgggcatcg gtttggcaaa caaaaatcct tgccgctcat
      961 tgatcccgtt cttggttaaa aaagcatctt cctttgcact ctctacacct tcggcgatca
     1021 cctgaagatt caatgcctgg gccacagcga cgatcgcccg gaccagtgac tgcgagacag
     1081 gttgtttgtg aatatctcga acaaaaacct ggtcaagttt gatggcatcg atcggaaagc
     1141 gcgccagttg cgaaagtgaa gagtagccgg taccaaaatc atccagatgc acttgcgcac
     1201 ctagttggct aaattgttga ataacagaca gtgccagttc atcattctca atcagacaac
     1261 tctctgtcag ttcaacatct atagggcagt attcaaaatt gagttcctgg agaacctgtt
     1321 tcagggcggt gaaaatggtt tgatcggcga gctgacgtgc agaaatattt accgccactc
     1381 gcaggtttat gcctttatcc cgccactttg ccacctggcg tacgacatcg agaatcaccc
     1441 aacggcctaa aggcacaatt agccctgact cttcggcgta ggaaatgaag tccaacggtg
     1501 gaatcaaccc acgttcaggt gactgccaac gtactagtgc ttccagactg cgcacttcgc
     1561 cacgctaggt gattttcgat aaccaactga tcgttttcca gtgctttacg caagttggta
     1621 tccagccaga gatattcaaa tacccgctga ttcatttctg gagtaaaaac gcaaaattgt
     1681 cctcgtccgc cttccttcgc tgtgtacatt gctgtgtcgg cgtgacgaat aatagtcgcg
     1741 ctgtccgaac catgttcggg agagagcgaa atacctactg aacagctggt ataaacttca
     1801 attaaaccaa tacgaaaggg gaggcgtaag cgggtcaaaa ttcgtgatgc cattgcttcc
     1861 agcgcgcttt gtgaggtatt ggatgccagt accagaaact catcgccacc tggacgcgcc
     1921 aacacctggt catgttcgag acagcttaaa atagccaatg acacgtcgcg taataactgg
     1981 tcaccaaaca aatgcccata ggcgtcgttg acctttttaa aattatccaa atcaagatac
     2041 acaaccccaa ctttattgtt atctgcctga ttaatagcgt gatcgattaa ctcctgcatt
     2101 gcgttacgat tcggcagtcc ggtgatactg tcggtatttg ccagaatacg cagtcgctcc
     2161 tgagtgcggc gctcttcggt aatgtcggta ccggaacaga ttaaaaaaat ctcgtttttg
     2221 ccactgccgc tgtggacaaa tttattgcga aacagaaaca gccgctggcc tttacgtgtt
     2281 ggtatccaca gttcgacttc atatgcattg ccgcttcgaa aaaatacacg gttattgcgc
     2341 ctggatgccg cagcttcacg acggctcata aacagtttaa acacgctttg cccaatgacg
     2401 tcgtgttctt tcaaccctgt gtaatcttca cataaccgat tgaagcgttg gatattcccc
     2461 cggctgtcga gaatcactat tgcggaatgt gcttcagaaa ctacctgctc agcaaaggaa
     2521 agcccgtgtg ataaatcacg agcaaccgcc ggtgtgtcat gccatgcaga cgcgttgcca
     2581 gcccattccc gtttattgat ttttcgtcct accagatgca cagaaagatc gctttcatcg
     2641 acggtcagac tcatcatcag gctggaggtg atgaccgtca tttccctgat gcgagcagcc
     2701 tgttcggcag ataactgcaa tgtacgatca ggttctgtgg ttttggtggt agaaaagcgc
     2761 aaagcatcgc tgctttccgt caaccgccag tatggattgt gcgatccgag aaagttgtac
     2821 aacgttgtgg actccctaac ggttttcatg atgcatatcc tccggttaac agcggagtcc
     2881 gcggttaaat aaaaggaaca acatgactta catgaaatta acggcggata aacgcattgt
     2941 tttttatata tttatttgta atccagtttt ggaaaaacgc cagttttcaa acgaaagtca
     3001 gttaaaaaat ctgcctggat ataacgaagg tagagcgggg aaataaacgg cccatccatg
     3061 aggaatgggc cgtgaaagga gattacgcga ccgggcgcgc aataatgctg cgggtttcca
     3121 tgcggacttc ggcaatggtg acgtcaataa cgtcagttac tttgtaaacc gtttcacctt
     3181 taatttgtac agtgccgttt tcctggctgc aaaccagttc atcgcgcaca gcgtgtaaga
     3241 aaggtgccgg aataaaggcg atagcgccgt tatcaaccaa acgcacacgc atgccgccac
     3301 ggctgatatc gacaatttcc gccgcgaaac gggtgccggt cccggcttta tctttcagga
     3361 agcgtgcgta taaccagtcg ccaacatcac gttctgccat ccggttgaga cggcgacgtt
     3421 cggccatttg gacagtgatt tcatcctgcg gacgcgtcgc agtttcgcct ttgataaccg
     3481 ctttcagcag acggtggttg atcatgtcgc catatttacg gatcggcgaa gtccaggtgg
     3541 cgtatgcttc cagaccgagg ccaaagtgag gaccgggttc agtgctaatt tcagcaaatg
     3601 actggaagcg acgaatgcgg ctgtcgagga aaccagttgg ttgcgcatcc agttcacgac
     3661 gcagtttgca gaaaccgtcc agcgtgagta cttcttcggc atcgacatgc agaccgtgcg
     3721 ttttcagcaa cgctgccagc gcgtcggcat tcgccggatc aaagcccata tgcacgttat
     3781 agatgccaaa accgagctta tcgcgcagta cgcgggccgc acaaatgtta gcggcaatca
     3841 tcgcttcttc gacgatacgg ttggcaatgc gacgaggctc ggcgacgata tccagcactt
     3901 cacctttttc accgagaata aagcggtaat ccgggcgatc tttaaacacc agtgcgtggt
     3961 tatgacgcca ctcgccgcgg cgttggcaaa tttgcgctag caaacggact tgttcggcaa
     4021 ttgcttcact ttcaggcttc cagtcaccgg tattttccag ccagtcagaa acctggtcat
     4081 acaccagctt cgctttggat tcgatggtgg cggcaaagaa ttcgatatta tcttcaatgg
     4141 tgccatcagc ggagagcgtc atgcggcatg ccagtaccgg gcggacttca ttggcgcgca
     4201 gtgagcagag atcgtcagaa agctcgcgag gcagcatagg gatgttgaag ccaggcagat
     4261 agttggtgaa tgcgcgaatt ttcgcggctt tgtccagctt gctgccttca gcaatccacg
     4321 cggttggatc ggcaatcgcc acaatcagct gaagtttgtc atccggcagc gccttagcga
     4381 aaagggcgtc atccatatct tcagtgctgg cactgtcgat ggtgacaaaa tccagcgagg
     4441 tcagatcttc acgaaccaga ccttcatcga gcatttcggt agcgacgccg tctggtgctt
     4501 ctttttccag attatggcgc gcaagggtaa cccaccacgg tacaaagtgg tcgtcgccaa
     4561 aagtgatgta ttgtgtcagt tcagcataga aagaacgatc gcctttcagc ggatgacggc
     4621 gcatttcggc aaccgcccag tcgccttctt taaactcgtg gttcaggcca cgggctgcgc
     4681 ggcaaggaat ggcgtctttt aaaagtggat gatcaggaac gatggtcaga cggtcatttt
     4741 tgccctgaac tttacccacg aaacgagtca ggaacggttc aaccagttct tctggctctg
     4801 cggattcacg ttctttttca ctgtggatca ccgcgataat tcggtcgcca tgcatgactt
     4861 ttttcatctg cggcggcgga atgaaataac ttttttgtgc gtcgacttcc aggaagccaa
     4921 agcctttttc tgtggctttt accacccctt cagcgcgtgg cgtctgggaa tgcagttgct
     4981 gtttaagctg cgctagcagc gggttgtcct gaaacataat tgtctatttt ggtggccatt
     5041 agagcggctg acagttttac gcgaatctgt ctgacgcggc aaggttaata tgtctcaccc
     5101 aacgcgattt ttaagcgatt tatccagcca cacagcccgc tccataccag cagattaata
     5161 atctgcgttg atgatttttc gtgttcgagt aaagggctga actgggcggc gctaaagcga
     5221 tcgggtgctc gggtcagcca ttgtatcgcc tgaacggtag cgcgctcaac agaatgacgg
     5281 tcagcccatc ggttaatttc attttcgccc ttgcgcagaa gggtgctgat ttttttatat
     5341 tcatttgatg aatccataca ggtggaaaaa caagcaggac tgccatttat acgcgcgcat
     5401 aacaacgccg caagctgatt atccggctgt gtattgaccc gtgtgctgtt taacaacttt
     5461 tcgagaaggg aaagtgaaat agggtggaga gagtgtgttt cggccagttg gcaaagttct
     5521 gcagttgaac tttttgtgtg ctcttcaggt agtttttcca cctcatagct gcttcgccag
     5581 cgtattgatt catcagcaaa cagtgacgcg tcggcgtagt tttgtatcgc cagcccgggt
     5641 aaccagcgta ccggatgccc gagataagcc tgaaatgtcg caattgtccg cgcctgaaag
     5701 ccaataaaac caatgatttg gttaatgatc acacagtccc agacagtaag accgacgtca
     5761 ttcagatgct ggcgcgatcg gttgtcgata atagcaggcg agctagccag ttggcgggca
     5821 tattgcgtga tttgtgccaa ccgatgatta ttttccctgg aggaatcagg tccggggagc
     5881 ggcgtaagtc gcgccgaata gtagttacat aaccgctgga cgccgcaaac ctgagcaacc
     5941 gtcagggccg tcgacaagcg ttcataagcg gtgaaggttt gtgaacgggt cacggtaagc
     6001 tggtcaggga acaactccac tgcaagaacg cgcgcaggga ccagccatga aaggaagggg
     6061 gccagcgtct cttcatcaag gatcacgtcg agcagaaagg gatcgctgac ctttgcggct
     6121 tccgggacca gaggcagaac gtcatactcc gtagtactgg attgcgtttc atgataccag
     6181 tggcttttgc cggtgatgtg gcgttgttcc atgggcgctc cttggtcgta aaggaaatcg
     6241 ttatcctgac gcaaggcggg aaggggagaa agacggatcg gcgataacaa atatcagaaa
     6301 ggtataacag agataacgcg ggcggcagaa cgccgcccat ctttaccaac agaacgatta
     6361 tttcagttcg agttcgttca ttgcagcgat gctgaaaccg ccgtcaacgt ggactacttc
     6421 accggagata ccggcagaga gatcggagca caggaatgcc gcagagttac ccacatcttc
     6481 aatagtaacg gtacggcgaa tcggggtaac ggcttcgcaa tgagccagca ttttgcggaa
     6541 gtctttgata ccggaggccg ccagagtacg gatcggacca gcagagatgg cgttaacacg
     6601 cacaccttcc ggacccatcg cgttcgccat atagcgcacg ttcgcttcca gagacgcttt
     6661 tgccagaccc ataacgttgt agttcgggat agcgcgctca gcgccaaggt aggaaagggt
     6721 cagcagggca gaacccggat tcagcatgga gcggcaagct tttgccattg caacgaagct
     6781 gtaggagctg atgtcgtggg caattttgaa gccttcacgg gtaacggcgt taacatagtc
     6841 accatccagc tgatcgccag gtgcaaaacc aatagagtgt acgaaaccgt caaatttcgg
     6901 ccaaactttc cccagttcag cgaacatggt gtcgatgctg gcatcttctg caacatcgca
     6961 ctgcagaacg atgtcagaac ccaattgagc ggcaaattct tctacgcggc ctttcagttt
     7021 gtcgttctgg taggtgaatg ccagttcagc tccttcgcgg tgcatcgcct gagcgatacc
     7081 gtaggcgatg gacagtttgc tggcaacacc ggttaccaga atgcgcttac cggaaagaaa
     7141 acccatagct ttaatcctta ttgttgatgc ttgttgtgcc tgaaaatcag gcgattcgtc
     7201 gttttagtaa acagtacgaa cagataaacg gttattataa tcaacctggc tgtgagtagc
     7261 tatagttgcc aggtccgacc ggagcaggct gcggcagggg gggcgctttt ccccttaccc
     7321 taaccctctc cccagagggg cgaggggacc atattgtgca aatattgtta ccccagcaac
     7381 aaacaggctc atacagcccc taaccctttt atggcgatgg ctgggatggt tcagaccttg
     7441 ccgaatattc tccagcactg tctccatgtt ttcaccacaa cagctattcg gctcggtcgg
     7501 ccccctcgcc ctttcagggt gagggtgacc ggcggttcag ttcctgcaga ataatctcca
     7561 gcaccgcctc ctcattacaa tcaatctcgt tattccagaa acgcagcacg gtccagccct
     7621 gcgactcaag ccagctagtg cgcctggtat cataggcaac tgccaaatca tgctgcccac
     7681 catccagctc aacgactacg cgcgccgagc agcaagcaaa atcgagaatg tagctcccca
     7741 ccggatgttg acggcgaaat ttgaaatcac caaaacgtcg gctgcgaaga tatcgccaga
     7801 gctttcgttc ctgcaaagtg agattgcgtc ttaaatcacg cgcatttgat tttattttat
     7861 ccatcacctc attctgacaa gatttaatct cttgtcagaa tgaggtgaat aatctggaag
     7921 gaggattcag aaaattagcg atctttacgc cacgcatccg ctgtcaatgc ttcgccaaag
     7981 tgcccggcaa tcagccgttt ggtgagttca tgcagcggcg atgccagcac atccgcggtg
     8041 ctgcctcgct cgacaacctc gccctgatgc atcaccagca cctggtcgct aatgtgcttc
     8101 atcattccga tatgctgggt aacataaata tacgaaatgc cctgtttttc ctgtaactcc
     8161 agcatcagat taatcaactg cgaacgcatc gacatatcca gtgaagcgag ggcttcatcg
     8221 gcaataatga cttttgggcg caatatcagc gcgcgcgcca gacccagacg ctgtttttgt
     8281 ccgggtgcca acatatgcgg atagtaactg acgtgatccg gtagcagccc aaccatacgc
     8341 atcgtttcaa taatctgttt gcgacgctgt tccggttcca ggtcggtgtt caggcgcagt
     8401 ggaaaatcca gaatttgtga gatacgttga cggggattca acgaggtcga aggatcctga
     8461 aaaatcatgc gaatacgctg actacggaag gaataatcgc caaaatgcag cggatgatcg
     8521 tcaatcaata actcgccgct ggtaggttct atcattcccg ccagcatttt tgccagtgtg
     8581 gatttacccg aaccattctc gccaataatc gccagtgtct ggccttcacg tagcgtaaag
     8641 ctcaagggtt ttaccgcttc tacggtctga cgacgaaacc aaccggtccg gtagcggaac
     8701 gttttactta aattacgcac ttcaagcagc gtttcgatca tctcactctt tctccatgtt
     8761 cagcgggaaa tgacaggcat agagatgatt tttcgccccc gtcaaacgtg gcgtcacaat
     8821 gcattctcgt tgtgcatacg ggcaacgtgg ccccagacga caaccaatcg gtaactgttc
     8881 cagcaatggg atagcgccgg gtagagtatt gaggcgactt ttatgcggca tcgcgctgcc
     8941 gaagtctggt atcgcgcgga tcagcgcctg ggtataagga tgatgtggca tcgtcaccaa
     9001 ctccttactc ggcgcggttt ccactgtttg accgcagtaa agcacgttaa ttttatccgc
     9061 ccattggcta agcatttgta agtcatggct gataagcaaa atagtggtat tgctgttttg
     9121 gttgagacgc gtcagcaggc gaaagatttg cgcctgggtt gttggctcca ttgagtttgt
     9181 cggttcgtca gcaatcagca gacgcggttg attcgccagt gcaatggcta tcatcacttt
     9241 ctgacattca ccttcggtca actcataggg aaaactgcgc atcgcatctt tgtgatcttt
     9301 aatccccacg cggtgcagca gttcaatggc acggcgtttg cgccagccaa aacgctgcca
     9361 ccaacggcct ttataggtcc aggctgggat gttttgcatc aactggcgac ccacacgttc
     9421 tgaagggtca agacacgact gcggttcctg gaaaatcatc gacacgttat ggccaaccag
     9481 tttgcgccgt tcgcgtgcgg agagacgcag caaatcgata tcatcaaaac gcatacggtc
     9541 agcagtaaca cgccagttat ctttattcac cccacaaatc gctttcgcaa tcaaactctt
     9601 gccggaaccg gattcaccaa caagaccgcg gatttcacct tcggttaagg tcatacttac
     9661 ccggtcgacg gctttaaccc actcatcacc ggttttaaat tcaatggtca ggttacgaat
     9721 atcgagtaat ggcattattc cacccccgca ataatcgcac gacgaacgcc atcgccaagg
     9781 aggttaacca acaacacgct aatcataatt gccgcacctg gcagcatgac agtccacggg
     9841 gcgacataaa tcagttccag cgcatcaccg agcatcgctc cccattcagg cgaggggagt
     9901 tgtgcgccga gatcgagaaa gcccagcgcg gcgatatcga gaattgccat cgacagtgcg
     9961 cgggtgatct cggtgaccag cccggcggtg atgtttggca tcacagcaaa ccagagaata
    10021 ttcagcgttg atgcgccatc cagacgggcg gcgataacgt actctttttc cagttcgtca
    10081 tgcaccatgc tgtaaatcga acgtaccata cgcggcagca atgccagcca gacagcaaac
    10141 atagcgtggg acaaactcgg tccggcaaac gctacaacga taattgccaa cagcagcgaa
    10201 gggatcgcca gtaaggtatc cagaatatgg ttaagcaccg ctgagcggag gccgtgtgtc
    10261 gccccggcga aggtgcccag taccaggcca cagatcgtcg cggcaagcgt caccacaaat
    10321 gcgccaccca cggttggcgc agctccgctc agtaaacggc ttaaaacatc acgtccgagg
    10381 tcgtcagtcc ccaggaagaa agaaacttcg ccatagcgcg accatgatgg cggcaataat
    10441 tgataaccga gaaattgctg gtcgatgccg taaggcgcaa accagccgcc aaaaatacac
    10501 agtacagcca gtcccgcgca gccgtacagg ccgaccatcg cagaggcgtc actataaaat
    10561 ttgcgccagg cggtacgcag cgtgcccggc gggcgctttt cgctgtatac gctatcgtaa
    10621 ggcataccat tccttatgtt tcagagggtt agccatggca cccaaaatat cagaaatcac
    10681 gttaacaata atgaccagtg agccacacac catcactccg gcggaaatgg ctgcgtaatc
    10741 ctgctggcgg atggcgttaa ttaaccagcg ccctaaaccc ggccagctaa aaaccatttc
    10801 ggtgatcatc gccagcgtca gcatggtgga aaactgtaag cccaggcgag gaataaccgg
    10861 cggtaacgcg ttatgcagaa cgtggcgacg caaaatagta aagcgcgaca aaccgcgggt
    10921 cgccgccgct tttacatagt tctggtcata cacttcaata gtgctgatac gcatcaggcg
    10981 gatcacttca gttgttggcg caaccgacag ggtgatcacg ggcaatatca tatggcggat
    11041 tgcgctcatg atcatttcat cccgccatgg tgagtccgaa agccaggcat caatcaacgc
    11101 aaaaccggta atcggtttca cttcgtagag cagatcgaaa cgccctgaaa cgggcaacca
    11161 acctagcgtg agtgaacaaa acagtgtcaa cagaagcgcc agccagaaca ccgggattga
    11221 aaagcccagt aaggcgatgg cgttgatcaa gttatcctgc cacttatggc gcgtaatccc
    11281 ggcaatcatc cccacgggga tgccaacaat cagcgcaaaa ccgaaggcga ggatgcacag
    11341 ttctatcgtc gccgggaaga cctcttttaa ctgttcggca attggttgac cgttaatgct
    11401 ggaaacacca aaatcccagt ggatcaggcc attaaaccag aacacccagg cattccacag
    11461 tgacgcgcct tgtaacggcg cgtgtggagt gaaatagctc aagctgaagc caacgaaggt
    11521 cagcaggaac aaggtgacaa tcaataacaa aatgcggcgt aaggtgaaga taatcatggt
    11581 tttttcacct catcctgttt ctcgcgatac accccagcaa aggaggcgtt accaaacggg
    11641 ctaagtacca gacctttgat atcgtaccga taggcctgca aacgcaatga cgacgccagc
    11701 ggcagaatag gtaattcctg cgccagaata ctctgcgctt cgtcataggc ttcaatacgc
    11761 gctgccagct gctgcgagga gagcgcctta cgcaacacgc tgtcgaattt cggatcgcac
    11821 cagtgggcga ggttggtctg tgaatgaatt gccgcgcagc tcagtaacgg acggaagaaa
    11881 ctgtccgggt cgttactgtc cgtcgcccaa ccggataacg tcagatcatg gctcatatcc
    11941 atcaaccgcg cctcctgaaa gcgaccttct accggcacaa tcaccacttt tacgtcaacc
    12001 tgcgccatat ccgcctgaat cagttcggca gttttcagtg gactggggtt ccacgcctgc
    12061 gaacgtgtgg gcacccacag tttcagcgtt aagttttcca gccccaacgc cttcaactgt
    12121 tcgcgcgatt tcgccggatt gtattcagta attttagcct cgttgtcata ggcccacgag
    12181 gcgcgcggta aaatagaggc cgccgtttca gccgtaccat aatagatgga ttgcatcagg
    12241 cgctggttat taatcgccag tgccagtgca tggcggacag cgggattatt tagcggcggt
    12301 ttggcggtgt taaatgccag ataggcgacg ttcatcccag gacgcagcgt taaacgcaag
    12361 cgcgggtcgt cacgcaaaat ggatagctgg ctggcagcag gccaggccag aacgtcgcat
    12421 tccccggtca ggagtttcga cagacgtccg gtgccgccgg agcctaaatc caccactacc
    12481 tgcggcatta acggtttacc gcgccagaag tcatcatgac gttgtaggcg aataaattgc
    12541 ccggcgcggt attccgacaa ctgatacgga ccggtgccga ccggttgacg gtcgagttgc
    12601 tcctggcgat cttctttctc taacttccgg gcatattctg ccgacatgac cgaagcataa
    12661 tgggttgcga ggtgccacaa aaaagaagca tccggctggg cgagtcggaa ctcaacggta
    12721 tgattatcca gtttgcggac gcttttcacg ttatcggcaa attgcaggct gtcgaagtag
    12781 gggaagttgc tgccgttgac gttatgccac gggttgttgc ggtcaaaaat tcgctggaag
    12841 gtaaacacca catcgtcggc attcatttta cgagtgggag taaaccagtc ggttttttga
    12901 aacggaacat cgcgacgcag gtggaagcga taggtcgcgc cgttgtcgag tacttcccag
    12961 ctttcggcaa gttccggcat caggcgatag gtatagggat cgacatccag cagtcgatca
    13021 taaaactggg cggcaagggt atcgacaatt aacccactgc tcgctttgga tgggttaaag
    13081 gtgttgactt gcccgctgac gcaatagaca aaaccgctgt cgcggatatc agcatgcggg
    13141 ggggattcag gcgcggcgat tgcctgacca ctcacaagtc cagcaatcac caaaagagac
    13201 gataatacct ggcgcataat attaagggat tttatgtaaa gaggctatct tactaatatt
    13261 taatgacatt tgccattacc gtttgtgttc aggggtcgta atgagggccg tgtggttggt
    13321 cgttgaacag gtggcgtcgc catctgttca acattcgtac ccgtgatttc tctataacta
    13381 taactcacag aacaacttag cgaggagcag agcggtacga tcgtgagaaa cccattaaag
    13441 cacgctgcag tgcgtgtcat tgttagccgg atgcggcgtg aacgctttat ccggacaacg
    13501 atactgaccg atcgtctgca attaaataat tactcattac cccattgatt caaaaactct
    13561 gcgatctcat caatgcgtac gggattaatc cccgcttcag cagccatttc atgttgggct
    13621 tcttcgctga tctcttcatt gttcatcaaa cgggtgagca gtaactggaa gtagtgggcc
    13681 agcggtgtgc gttcagcctc tgcgacaggc tctgcgcatt cggtcgcgta ctcatcggca
    13741 atatcaaaat attttagcgg gatatcgtgg ttcattattt gcccctgggg ttaatgcgct
    13801 aagcataagg gcggatgata gcactcgtga tccgggtcat acatcttttt atagttaatg
    13861 gcgcggcgcg ttaccctcag gcaggataat gcgccgcgca tccggctatt acgtttcgct
    13921 caccggcgtt ctgtcacctt cgtacagcgg cactttgcga tagcggcgga tcaggtacca
    13981 caaatatgcg ccgcctaacg aagcccatac cagaccgagt gtcagtgacg ttgactctag
    14041 gttaacccac agcacaccca ccgtcagcgc gccaaccagc ggcatcagca aatagtggaa
    14101 gtgatccttc cagcttttat tcattccttt acgccgccag aaatgattaa acaccgacag
    14161 attcacgaag gtaaacgcca ccagagcacc gaagttaatc agtgctgtcg cggtgactaa
    14221 atcaaagaac agcgccgaca gcgcgacaat cccgaccata atgacgttga gtgccggagt
    14281 ccgccatttt gggtgcacat agccaaacac gcgctctgga aacacattgt cacgccccat
    14341 cacatacagc agacgcgaca cgctggcatg tgaagccagg ccagacgcta atgtgttcac
    14401 aaacgtggtg cagaggaaaa tcgactgaaa gagcttgccg ccgacgtaca gcgcaatttc
    14461 aggcagtgcg gcgtccgggt ctttaaagcg gctgatatcg ggaaagaaca gctgcataaa
    14521 gaacgacgcc gcgataaaga taacgccgcc gtataccgcc gtcaggaaga tggctttcgg
    14581 gatcacgcgt gcggcgtccg gcgtctcttc cgaaagcgtg gtcaccgcat cgaaaccgag
    14641 gaacgagaaa cagacaatcg tcgcaccggt aataatcggg atcaggtgcg cgttctcgct
    14701 gataaacggc tgaagtgacc agacggtgcc aacgccttct cctttatgca gtccctgaac
    14761 caccaggaag ataaacacca ccatgatgga gatttgcacc agtacaaaca gggtattgaa
    14821 gttagcgacc aggttgacgc tcttcagatt cgcggcggtt aaaatggcga cgaaggttac
    14881 cacccacacc cacggcggca cttccgggaa gagggcggag agatagattt tcgccaacaa
    14941 aacgttgatc attggcaaaa agagataatc cagcagtgat gaccagccga ccataaatcc
    15001 gacgtgcggg ttaatcgact tttgcgcgta ggtataggcc gaaccggcct ccggaaactg
    15061 gcgaaccagt ttgccgtagc tgatagcggt aaacagcacg cccgccagcg ccagcaaata
    15121 ggacgccgga acgtgaccgt cgctaatgcc ggagacaata ccaaaagtat caaatacggt
    15181 catcggcgtg agataggcca gacccatcat taccacctgc cacaatttca gtgattttcg
    15241 cagacgggtt ttgccgggtt gcgcagcaat attcagtggt gaattaatag ccataattgc
    15301 tgcctcccct gcgtaataag gtttgcggtc gtgtcgacct gaaatcaaac ctgtttagca
    15361 agggaagcaa agggggcgga gtccagtcag tagcaggagg ggcgcatacg cagcctccct
    15421 cgccatgcgg ggatttaatc atcggcatca tctcatttcc ttgtcacagt cttaatcgtt
    15481 aacggaagcc cgttgccgcc tgcgctccat aacgcgacat ccgggaaatg tctggattac
    15541 cagcaaatgt caggcctggc tccgctcagg ccgatgaaac aaccccgcaa ggggaattac
    15601 gcgtttttca acatccactc aatttctgtt tctgtgatga ggcgctcaaa ctgcaacagc
    15661 tcatcatttt tacaggcgtg atacacatgg caaaaccgtt cgcctaaata gcggcgcagg
    15721 tgatcattct cgataaactc acccagggca tcgctctggc gaatcgggaa gggtaagcct
    15781 tcctgttcca gcccgttgcc ttcgacttct tcctgcaacg gcagctcgtt atcaaggcca
    15841 tgcaaaatac cggcaaaaat cgctgccatc accagatatg ggttggcatc ggcacccgcc
    15901 acgtgatatt ccacgcggtg attatgacgg tcaccgcacg gaatacgcag ggcgacggtg
    15961 cggttgttat ggccccacga cgcctgcgtc ggcacataca ttcccggctg gaagcggcga
    16021 tacgagttca cgtttggtgc cagcaacgcc atcgacgacg gcatcaggtc aatcatcccg
    16081 gcgagcatct ttttcagcag cggcgaatct tcgccttctg tgtcagaaag cacattctcg
    16141 ccacgattat tttgcatact gatatggata tgcatcccgc tgcccgcgtg ctcttcatac
    16201 ggcttcgcca taaaagtggc gtgcatctta tgcttttctg ccatcagacg caccaggcgt
    16261 tttagtgcca gcgcatcatc gcaggcttcc agcacgttat cagtatggta aaggttgatt
    16321 tcaaactgac ccggcgaggc ctcagcgacc gcgccatctg ccggaatcag ctgcaactgc
    16381 gccagttcat caatatcatt gagcacgtcg gcgaagtggt tgaggttatc aacggagtaa
    16441 acctggcttt gtgtattgcg gtcatcggtg ccaggcgcgc agggcggttg cagatcccct
    16501 tcagcgtcgc gctggcgatc cagtaaatag aactccagct ctaccgctac aaccgggaac
    16561 aatccgcgct ggcgcagctg ctgccacagg cggttaagaa cgttccgcgg ctcaacgtca
    16621 aagggagcgc catcttcatc gaccatggtc aggagcatct gaccgataaa ctctggatcg
    16681 gcggcagaag gagttaagga accgagaaca ggaacacagg tacgatccgg ctcgcccatt
    16741 tcctgaccca gacctgcttc ttctactacg ttgcccagaa tatccattgc aaataccgag
    16801 gccgggaaat aacacccttt ctcgagcttc ttcaggcttg aaacagggat acgttttcca
    16861 cggaagcaac cattcaaatc agtaagtaaa acatcaacat attgcgtatt cgggtagcgc
    16921 tccaggtagc gtttcacttc ctgcgtaaag gcgctacccc gcctctcttc tgactgctga
    16981 acaaagttct ctacttcaac gatattggtt tccatgattc ttcgcctttg gtttgttttc
    17041 cgctcgttat caaagcgtaa aatataatga ccatcattcg aatctgtatg caaaccaaat
    17101 gtttgtcaaa tgttaaattt ggtttgcaaa aatgaaaatc cactgctaga ttgaaaaaat
    17161 attgaacata aaggtcattt aaagcgcagt aatggcgata atttagtcca ctttgtgaga
    17221 ttgagcatgg aaaatataat gaacaatccg gttatcggtg tcgtaatgtg caggaacagg
    17281 cttaagggtc atgcgaccca gactctgcaa gaaaagtacc tgaatgccat catccacgca
    17341 ggcggcttgc caattgcgct accacatgcg ctggcggaac cgtcgttact tgaacaactt
    17401 ttgccgaaac tcgatggcat ttatcttcct agtagtccca gcaatgtgca gccgcaccta
    17461 tatggtgaaa acggcgatga gcctgacgcc gatcccgggc gtgatcttct gagcatggcg
    17521 ataattaatg ccgcactcga aaggcgcatc cccattttcg ccatctgccg gggtttacaa
    17581 gaactggtgg tggcaaccgg tggttcgttg catcgcaagc tgtgcgaaca gcctgaattg
    17641 ctggagcatc gggaagatcc cgaactgccg gtggaacagc aatatgcacc gtcgcatgaa
    17701 gttcaggttg aagagggggg attactgtct gcgttgttac ctgaatgtag caacttttgg
    17761 gtaaactctc tacatggaca aggggcgaag gtcgttagcc cacggttgcg tgttgaagct
    17821 cgctcgccgg atggcttagt tgaggcggtt agcgtcatca atcatccttt tgcgctgggc
    17881 gtacagtggc acccggaatg gaacagtagc gagtacgcgc tttcgcgtat attgttcgag
    17941 ggctttatca ccgcttggca gcaccatatc gctgaaaaac agcgactctg accactacag
    18001 tttaaggaaa tgcaaatatg agtgatgagg gactggcgcc aggaaaacgc ttgtcggaaa
    18061 tccgccagca gcaggggctt tcacaacgtc gtgccgccga actctccggg ctgactcaca
    18121 gtgctatcag tacgatagaa caagataaag tcagccctgc catcagtacg ctgcaaaagc
    18181 tgctgaaggt gtatggtctg tcactctcgg aattcttttc cgagccggaa aaacctgatg
    18241 agccgcaggt cgtcattaat caggacgact taattgagat gggcagtcag ggggtgtcaa
    18301 tgaagctggt tcataacgtt aacccgaatc gcacgctggc gatgatcttt gaaacgtacc
    18361 agccgggcac aaccactggg gaaagaatta agcatcaggg tgaggaaata ggcacagtac
    18421 tggaaggtga aattgttctg acgattaatg gtcaggatta ccacctcgtc gcggggcaaa
    18481 gttatgccat taataccggc atcccgcaca gtttcagtaa tacgtcggca ggtatttgcc
    18541 gaattatcag cgcccatacg cccaccacgt tttaatcttt ttgttctgta agccgggtaa
    18601 gcgcaatgcg cccggcaatc cataaagaat gtcgccagaa ataaacgggc atgcagcccg
    18661 gggatctctg cgccctgacg ttcacaaact gcatatatct gatagacgtg aaacaggagt
    18721 cataatgaat tttcatcatc tggcttactg gcaggataaa gcgttaagcc tcgccattga
    18781 aaaccgctta tttattaacg gtgaatatac tgctgcggcg gaaaatgaaa cctttgaaac
    18841 cgttgatccg gtcacccagg caccgctggc gaaaattgcc cgcggcaaga gcgtcgatat
    18901 cgaccgtgcg gtgagcgcag ctcgcggcgt atttgaacgc ggcgactggt cactctcttc
    18961 tccggctaaa cgtaaagcgg tactgaataa actcgccgat ttaatggaag cccacgccga
    19021 agagctggca ctgctggaaa ctctcgacac cggcaaaccg gattcgtcac agtctgcgtg
    19081 atgatattcc cggcgcggcg cgcgccattc gctggtacgc cgaagcgatc gacaaagtgt
    19141 atggcgaagt ggcgaccacc agtagccatg agctggcgat gatcgtgcgt gaaccggtcg
    19201 gcgtgattgc cgccatcgtg ccgtggaact tcccgctgtt gctgacttgc tggaaactcg
    19261 gcccggcgct ggcggcggga aacagcgtga ttctaaaacc gtctgaaaaa tcaccgctca
    19321 gtgcgattcg tctcgcgggg ctggcgaaag aagcaggctt gccggatggt gtgttgaacg
    19381 tggtgacggg ttttggtcat gaagcggggc aggcgctttc gcgtcataac gatatcgatg
    19441 ccatcgcctt taccggctcc acgcgtacgg gtaaacaact gctgaaagac gcgggtgata
    19501 gcaacatgaa acgtgtctgg ctggaagcgg gcggcaaaag cgccaacatc gttttcgccg
    19561 attgtccgga tctgcaaaaa gcggcaagcg ccaccgccgc aggcatcttc tacaaccagg
    19621 gccaggtgtg catcgccgga acgcgtttgt tactggaaga gagcatcgcc gatgaattct
    19681 tagccctgtt aaaacagcag gcgcaaaact ggcagccggg ccatccactt gatcccgcaa
    19741 ccaccatggg caccttaatc gacagcgctc acgctgactc ggtacacacc tttattcgtg
    19801 aaggcgaagg caaggggcaa ctgctgctgg atggtcgcaa cgccgggctg gcagcggcca
    19861 tcggcccgac catcttcgtg gatgtggacc cgaatgcgtc cttaagccgc gaagagattt
    19921 tcggtccggt gctggtggtc acccgtttca catcagaaga tcaggcgctg cagcttgcca
    19981 acgacagcca gtacggcctt ggcgcggcgg tatggacgcg cgacctctcc cgcgcgcacc
    20041 gcatgagccg acgcctgaaa gccggttccg tcttcgtcaa taactacaac gacggcgata
    20101 tgaccgtgcc gtttggcggc tataagcaga gcggcaacgg tcgcgacaaa tccctgcatg
    20161 cccttgaaaa attcactgaa ctgaaaacca tctggataag cctggaggcc tgaaatgacc
    20221 gaacatacca gcagttacta cgccgccagt gcgaataaat atgcaccatt cgatacactg
    20281 aatgagtcaa tcacctgcga cgtttgcgtg gttggcggcg gctataccgg gctctcctcc
    20341 gcgctgcatc tggcggaagc gggctttgac gtagtggttc tcgaagcctc acgcatcggc
    20401 tttggcgcaa gcgggcgcaa tggcgggcag cttgtgaact cctacagccg cgacatcgac
    20461 gtgatcgaaa aaagctacgg catggacacc gcccgtatgc tcggcagcat gatgttcgag
    20521 ggtggcgaga tcatccgcga acgtatcaaa cgttatcaga ttgactgcga ctaccgcccc
    20581 ggcggtctgt ttgtggcgat gaatgataaa caactcgcca cactcgaaga gcagaaagag
    20641 aactgggaac gctacggcaa taaacagctg gagctgctgg acgccaacgc tattcgccgc
    20701 gaggtcgcca gcgatcgcta cactggtgcg ctgctggatc acagcggtgg gcatattcac
    20761 ccgctaaacc tcgccattgg tgaagccgat gccatccgcc tgaacggcgg gcgcgtgtat
    20821 gagctttccg ccgtgacgca gatccagcac accacgccag ccgtggttcg gactgccaat
    20881 ggtcaggtga cggcgaagta tgtgattgtc gccgggaatg cgtatctggg cgataaagta
    20941 gagccggaac tagcgaaacg cagcatgccg tgcggcacgc aggtgatcac caccgaacgg
    21001 ctgtcggaag atttagcccg ttcgctgatc ccgaaaaact actgtgtgga agactgtaac
    21061 tatctgcttg attactaccg tcttaccgcc gacaaccgcc tgctgtacgg cgggggtgta
    21121 gtgtatggtg cacgcaaccc ggatgacgtt gagcgtcttg tggtaccgaa actactaaaa
    21181 accttccctc agttgaaggg cgtgaaaatt gattaccgct ggacgggcaa cttcctgctg
    21241 accttgtcgc gcatgccgca gttcggccgc cttgatacca acatctatta catgcagggt
    21301 tacagcggcc acggcgtcac ctgtacccat cttgccgggc gtttgattgc tgaactgctg
    21361 cgcggtgacg ccgagcgttt cgatgccttc gccaatctgc cgcattaccc gttccccggc
    21421 gggcgcacgc tgcgcgtgcc atttaccgcg atgggcgcgg cttattacag cctgcgcgat
    21481 cgtctgggcg tttaatttcc gattaacagt gaagagtcaa aaggtgtgaa acatgagcaa
    21541 caatgaattc catcagcgtc gtctttctgc taccccacgc ggggttggcg tgatgtgtaa
    21601 cttcttcgcc cagtcggcag aaaacgctac gctgaaggac gtagagggca acgaatacat
    21661 cgatttcgcc gcaggcattg cggtgctgaa taccgggcat cgccactctg atctggtcgc
    21721 ggcggtggag cagcaattgc aacagtttac ccacacggcg tatcagattg tgccgtacga
    21781 aagctacgtc acgctggcgg agaaaattaa cgcccttgcc ccagtgagcg ggcaggctaa
    21841 aaccgcattc ttcactaccg gcgcggaagc ggtggaaaac gcggtgaaaa ttgctcgcgc
    21901 ccataccgga cgacctggcg tgattgcgtt tagcggtggc ttccacggtc gtacatatat
    21961 gaccatggcg ctgaccggaa aagttgcgcc gtacaaaatc ggcttcggcc cgttccctgg
    22021 ttcggtgtat cacgtacctt atccgtcaga tttacacggc atttcaacac aggactccct
    22081 cgacgccatc gaacgcttgt ttaaatcaga catcgaagcg aagcaggtcg cggccattat
    22141 tttcgaaccg gtgcagggcg agggcggttt caacgttgcg ccaaaagagc tggttgccgc
    22201 cattcgccgc ctgtgcgacg agcacggtat tgtgatgatt gccgatgaag tgcaaagcgg
    22261 ctttgcgcgt accggtaaac tgtttgccat ggatcattac gccgataagc cggacttaat
    22321 gacgatggcg aaaagcctcg cg
//
LOCUS       DAJTYF010000060        22319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTYF010000060 DAJTYF010000000
VERSION     DAJTYF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.060"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="OO590_002378"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="OO590_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6637715.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            831..971
                     /locus_tag="OO590_002379"
     CDS             831..971
                     /locus_tag="OO590_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637716.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            985..1857
                     /locus_tag="OO590_002380"
     CDS             985..1857
                     /locus_tag="OO590_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR6637717.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKNETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1917..3215)
                     /gene="eno"
                     /locus_tag="OO590_002381"
     CDS             complement(1917..3215)
                     /gene="eno"
                     /locus_tag="OO590_002381"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6637718.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="OO590_002382"
     CDS             complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="OO590_002382"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6637719.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="OO590_002383"
     CDS             complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="OO590_002383"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6637720.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6055..8289)
                     /gene="relA"
                     /locus_tag="OO590_002384"
     CDS             complement(6055..8289)
                     /gene="relA"
                     /locus_tag="OO590_002384"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HCR6637721.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="OO590_002385"
     CDS             complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="OO590_002385"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6637722.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9695..12451
                     /gene="barA"
                     /locus_tag="OO590_002386"
     CDS             9695..12451
                     /gene="barA"
                     /locus_tag="OO590_002386"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6637723.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="OO590_002387"
     CDS             complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="OO590_002387"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6637724.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14044..15384)
                     /locus_tag="OO590_002388"
     CDS             complement(14044..15384)
                     /locus_tag="OO590_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6637725.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15386..16738)
                     /gene="gudP"
                     /locus_tag="OO590_002389"
     CDS             complement(15386..16738)
                     /gene="gudP"
                     /locus_tag="OO590_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6637726.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17172..17621)
                     /locus_tag="OO590_002390"
     CDS             complement(17172..17621)
                     /locus_tag="OO590_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6637727.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17639..18421)
                     /gene="truC"
                     /locus_tag="OO590_002391"
     CDS             complement(17639..18421)
                     /gene="truC"
                     /locus_tag="OO590_002391"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6637728.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTVHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18421..18750)
                     /locus_tag="OO590_002392"
     CDS             complement(18421..18750)
                     /locus_tag="OO590_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6637729.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19372..19917)
                     /gene="syd"
                     /locus_tag="OO590_002393"
     CDS             complement(19372..19917)
                     /gene="syd"
                     /locus_tag="OO590_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6637730.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRMHLASNLAEFLNQLKPLL"
     gene            19985..20833
                     /gene="queF"
                     /locus_tag="OO590_002394"
     CDS             19985..20833
                     /gene="queF"
                     /locus_tag="OO590_002394"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6637731.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20945..22309
                     /gene="ppnN"
                     /locus_tag="OO590_002395"
                     /gene_synonym="ygdH"
     CDS             20945..22309
                     /gene="ppnN"
                     /locus_tag="OO590_002395"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6637732.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5512 a         5916 c         5292 g         5599 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat atcgttaata attaaaggga gtaacgtatt atgtcagaag
      841 aaaataaaga aaatggattt aatcatgtca aaatattcac caaaattata tttatttttt
      901 ctgtattagt ttttaatgat aacgaatcta aaattaccga tgccgccgtc aatttattta
      961 tccagattta atgagaaatt tataatgcgc tatttcatac taatgttcac tttcgtatgt
     1021 tcctttgttg cagcccaacc aactattgtc ccacaattac agcaacaagt tacgggtctt
     1081 acgagtagct taaactcaca agaaaagaaa gaactgacgc acaagttaga atctattttc
     1141 aataataccc aagtgcaaat tgctgtatta attgtttcca caaccaaaaa tgagacaatt
     1201 gaacaatatg ctacaagagt ttttgacaat tggcgtttag gagatgccaa acgtaatgat
     1261 gggatactga tcgttgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg
     1321 ctggaagaaa aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc
     1381 gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac
     1441 caactcactt cacaacacca atatccggct aatccttcag aaagtgaatc aacgtcttcc
     1501 actgatcatt attactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg
     1561 ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca
     1621 atccatcttt tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc
     1681 ttctttttta ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga
     1741 gcatctggta gaagttatca ttcagacaac agcggttccg ctggaggctc aagtggcggc
     1801 ggatttagcg gcgggggagg ttcttctggc ggtggcggcg catccggccg ctggtaactc
     1861 caccataaaa aatgccagcc cgaaggctgg cattatcaaa tcagataaag tcagacttat
     1921 gcctggcctt tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg
     1981 atacgaatca gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg
     2041 atctggcctg cagcagtacc aacagccaga tcagcgatgg tagcatcttc agtttcacca
     2101 gaacggtgag agataactgc agtgtagcca gcatctttcg ccatcttgat tgcagccaga
     2161 gtttcggtca gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt
     2221 tcgatacctt ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg
     2281 attttgtcgc ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga
     2341 ccgtcttcga tagaaacgat cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat
     2401 tcttcagagg tgaacgcttt gttgccttcg ccagccagaa cgtatttacc atctttgtag
     2461 aattcagaag ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca
     2521 gctttaacag cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca
     2581 tagccacctt cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccagg
     2641 tgatggaaaa cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc
     2701 tgaatcatga attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg
     2761 ttcatcatcg gaaccggcat ggagtatttg cccggagtgc cgttcagttc agcgatgtgc
     2821 tcgtacagcg gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc
     2881 aggattgcgt tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc
     2941 ttgtcaatgc cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg
     3001 tttaccgcag caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg
     3061 cgcagttcca gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg
     3121 acgaaaccac cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg
     3181 atttcacgac cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta
     3241 aactgaaact ccagacaaac aacgcgtacc caaggtacgc gttgccgttc taactttttt
     3301 acttacttcg cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc
     3361 gggtgaccat cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac
     3421 gggtgattcg gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccacaacg
     3481 cgcagacctg catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga
     3541 tgacgctcaa caattgtcgg cgcattgtat agctggcgaa ccaggctatc gtcaaccaac
     3601 tggcactgct gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact
     3661 tcaacgttgc cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag
     3721 tctggcacaa attctgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca
     3781 attaacgcca cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca
     3841 aaacgcgcgg tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg
     3901 attgcgtcca gacctttaag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc
     3961 agtttgatgt tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcact
     4021 gatttataag catccggcag ttcaatgtac ttgccgacca taccgatggt gacttcactt
     4081 accggattcg cttcttcgaa gataacctgt tcccattcgg acagatttgc ttccgggcag
     4141 tttaagctga atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga
     4201 attttataga tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag
     4261 aacaatgcaa tcttcgcacg ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg
     4321 atgtcaggct ggataccgat ggagagcagc tcttttacag agtgctgagt cggtttggtt
     4381 ttgacttcac cagacgctgc catgtacggc accagcgtca ggtgcataaa cagagtgtgc
     4441 tcacggccaa tttcaacagc catctggcga atagcttcga ggaacggcaa ggattcgata
     4501 tcacctactg taccgccgat ttctaccagt actacgtcat gaccttcgcc accttccagc
     4561 acgcgctctt tgattgcgtt agtgatgtgc ggaataacct gcacggttgc gccgaggtag
     4621 tcaccgcggc gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg
     4681 cggcggctca ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca
     4741 gcgccgtctt cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg
     4801 acgttgatgt aaggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg
     4861 gctgcgaggg aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca
     4921 aaaatatagt tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa
     4981 ccaagacggg aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc
     5041 ctcttcgcca gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt
     5101 ttttgacgca aatcaagcgc ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt
     5161 aattccctta gagatcaatt tcctgccgtt ttacctgttg ccagacttct tccattgttt
     5221 cgaggtcaac acctgtcatt tccagtccac gcgcggcaac aatacgctcc acttcgcgaa
     5281 aacgacgctc gaatttttcg ttcacttttt gcaatgcgat ttctgctttc gtccctaaat
     5341 ggcgagccag attaaccgtg gcaaacagca ggtcccccat ttcctcctcc agtttagcct
     5401 ggtcgacaac agcctgccgc gcctcgtaca tcacctcgtc gatctcttcg tagactttat
     5461 caaccaccgg gccaagcgtc gtccagtcga agccaacgtt ggcgcaacgt ttctggattt
     5521 tttgcgcccg cattaaagcc ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg
     5581 ctttctgcgc gcgctcttcg gttttgattt gctcccaacg ggcaagcact tcactactgt
     5641 tttcggcaga actatcagca aaaacatgcg gatggcgacg ctctaattta tcgctaatag
     5701 cagcgcaaat atcattaaag tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca
     5761 ccacctggaa tagcagatcg cccagttcac cgcgaagatc gtcaaaatct tcacgggcga
     5821 tggcgtccag cacttcgtag gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct
     5881 gctctttatc ccacgggcag ccgttttccg gatcgcgcag gcgctgcata atagtgagca
     5941 aacggtcgat ttgattcatt gaattgtcct gaaaattgcg ggtctgtcag gtgtaatacc
     6001 ttcgagtaaa agatatcgct ggatacggct gacgccatat ccagctatgt aagactaact
     6061 cccgtacaac cgacgcgcgt cgataacatc cggcacctgg ttgagtttac ccagcacgcg
     6121 ccccagcact tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt
     6181 ggtgtcgcta cggctggcaa cgccaagcac gttcaccttc tcgttggcga gaatggtcgt
     6241 gatatcacgt aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc
     6301 ggcggagtag ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag
     6361 ttccgccagt tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa
     6421 gccgacaatc tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt
     6481 accaacacct tcaaccacca cacgaccgtt atctttactg cggttttgcg gtgtgtagct
     6541 tttttgctga agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa
     6601 ttgcgattgc aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc
     6661 cagcaactcg tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt
     6721 caggctgatc cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt
     6781 tttgtcacgg tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt
     6841 gacgtaaccg aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat
     6901 aatttcaatc tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat
     6961 ttttgccccg atgcagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg
     7021 cgttgatccc gcaggcaaat caacgacatc acctttcggc gtaaagacat acacccggtc
     7081 gtcaaacacc tgactacgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg
     7141 ccacgcaatc agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc
     7201 agctgccgcg ccctctttat atttccagtg cgcagcaaca cccaactccg catcttcatg
     7261 catctgtttg gtgcggattt ggatctcaac agtttttcca cccggcccca gaaccacagt
     7321 atgaatagac tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg
     7381 caggtggcga tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc
     7441 ggcgacaata cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg
     7501 cattttgcgc cagatgctgt agatgtgttt cggacgacca tacacttcag ctttaacgcc
     7561 ttcagctttc atctcagcgc gcagatggcc gacaaactct tcgatatagt gttcgcggtc
     7621 gagacggcgt tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg
     7681 gaagcagtaa tcttccagct cccatttcag ttgcccaatc ccaagacggt tcgccaacgg
     7741 cgcgtagata ttggtgcact cttttgccgc cagtacacgt tcatcttccg gcgcatcttt
     7801 tacttcgcgc agatgagcaa tacgctccgc cagtttgatg actacgcagc gaaaatcatc
     7861 gaccatcgcc aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt
     7921 gtgcgtcgct ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac
     7981 aaccgactta ccgacgctct cacgcagcac atcttcgctg actacgttgg catcagccag
     8041 agggaacagc agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc
     8101 caccatctca acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg
     8161 cagacaatac gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag
     8221 acttgcgatc catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac
     8281 cgcaaccatc gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgtaactat
     8341 ttaacgcgcg agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc
     8401 atcgccagtc gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc
     8461 gttgcagggt tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc
     8521 ataacacctg cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc
     8581 gcccacggct gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt
     8641 aagccattaa gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca
     8701 ccgaccacac tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat accgcagaac
     8761 agatccagta cgcgatcttc aggttgcaca tccagccatt ccaacgcacg cgctaccatt
     8821 ttttggttca cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac
     8881 ccgtttgagt cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg
     8941 gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc
     9001 gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt
     9061 tcaacatgac caagatggcg catagcttgt aagctgccca gacatgccct gactttgggc
     9121 agcaatgctt caagttgggg cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg
     9181 gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt
     9241 aaacgcgcgc ggcggcgata gccccaggga acatcggcga tcacttcaga gacatcgtgt
     9301 ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatccac gctggcgtgt
     9361 tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg
     9421 ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc ttcagtaaca
     9481 gcaacttccg cgttttcctg cggcaataat ccggggataa ataacgtttt gccgttatgt
     9541 cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc
     9601 gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa
     9661 cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg
     9721 catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt
     9781 cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgccggtg ctagcattat
     9841 tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg
     9901 tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta
     9961 tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca
    10021 gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat
    10081 gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga
    10141 tgccaaaaat agtcaaaata tgttgggata tattgcgctt gagctggatc ttaaatcggt
    10201 tcgcttgcag caatataaag agatctttat ttcctgcgtg atgatgctgt tttgtatcgg
    10261 tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat
    10321 ggtgaatacc gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat
    10381 gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc
    10441 ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct
    10501 ggagcagatg gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc
    10561 ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa
    10621 tggtgttatt ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga
    10681 tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct
    10741 cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat tcccactacg
    10801 cagcacgctg gatgaagtcg ttactctgct ggcacattct tctcacgata aagggttgga
    10861 actgacgctt aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt
    10921 acagcaaatc atcactaacc tagtggggaa tgcaattaaa ttcaccgaga atggcaacat
    10981 tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat
    11041 tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg
    11101 acaggctgat gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac
    11161 acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg
    11221 ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc
    11281 atccatccag tgcctcgcag gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc
    11341 gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt
    11401 ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg
    11461 cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct
    11521 gatgctggca cttccttgcc atgcacaagt caatgctgaa aaactcaagc aagatggtat
    11581 cggcgcgtgt ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt
    11641 ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac
    11701 agtcatggcg gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga
    11761 tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcagttgaac gggcgaaaca
    11821 gatgccgttc gatttgatct taatggatat tcaaatgccg gacatggatg gcattcgggc
    11881 ctgcgaactc atccaccaac tcccgcatca gcgacaaacg ccggttatcg cggtaacggc
    11941 gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc
    12001 gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg
    12061 tatttcgtct cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac
    12121 cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat
    12181 gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt
    12241 tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta
    12301 tagcggcgta ccacgtatga agaatctctg ccagctgatc gaacaacagc tacgtagcgg
    12361 tactaaagaa gaagatttgg agccggagct gctggaactg ttggacgaga tggataatgt
    12421 cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt
    12481 tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt
    12541 aacgccttat ccggcctaca aaaccaatta aattcaatga attaaaaaat gatgtagacc
    12601 tgatacatag cacaacaggc tattttgcgt ttagcatcag tctcaaaccg gctccagata
    12661 gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc
    12721 agccaggaat cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct
    12781 gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt
    12841 tttctggcac ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat
    12901 taccttcctg ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg
    12961 caacatgggt aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt gaaccccagg
    13021 tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcata gtccagaaat
    13081 gcggatccgc cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc
    13141 agtcggtggc gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca
    13201 tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg
    13261 aacctttcag gtatttacca attttaattg cttcgttcag cgaccaggca ccgttaggat
    13321 cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcttctt
    13381 cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg
    13441 ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat
    13501 accagtcgca tgagtcatcc ggctggcttt gatacggcag aggtgtggct ttgcgattac
    13561 cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca
    13621 gcgatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc
    13681 cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccactagcat
    13741 cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt
    13801 tacctaccac cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa
    13861 tttcccctac gccagtgtga ccggaattat ctttgataat cacaatatta cgcgtgaaga
    13921 acggtgcgtg tgcaccactc agattcatca gcatactgtc atgacccgcc accgggatga
    13981 cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca
    14041 gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta
    14101 ctgcatcgga cctgcgtcgt tacgcgcacc gcaaggcaga cgtttatagg cctcatgtgc
    14161 cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat
    14221 ttttccgttt ttaatctcca gcggattttt ggtcaggcga caatcgccct cctgccaaat
    14281 ccagtgggta tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaatat
    14341 cgccagagag atatcgaaat ggttgttgga atggcagccc caggtcagcc cccagtcgtc
    14401 gcaaagctgc gccacgcgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg
    14461 aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat
    14521 gttagtcgcg acgggcaagc cggtcgcccg tcgaaactcc gccatcactt cacgtccgga
    14581 gaagccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt
    14641 gcataaagaa atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc
    14701 atccgggaag cttttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac
    14761 cccgccctta agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg
    14821 cacaacggct tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc
    14881 cggcgtattt tccagataag gaagatcggt tttggtccga tcacctatat aaaacagata
    14941 accgaggacg gtaatagtct cgcgttgctt gcctggcccc aacagttcgc agaccggaac
    15001 attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac
    15061 gcgcaattca aaagtccagg cacctttgcc gaaggtctca aaatcggctg cctgatttcc
    15121 tttatgtacc tgctgaacca ctttattcag gcgcgcaact tcctggccca gaaccatcgg
    15181 aatagcatcg accagtgttt gataaatcac ctctccgccc ggcgcttcac caatgccggt
    15241 atgcccggcg ttatcggtga gtaccacgat attgcgggtg aagtatgcgt tatgcgcacc
    15301 accaatattg agcaacatgc tgtcatgtcc ggcgaccgga atgactttca tatcagtaat
    15361 aacaggactg gattgtgtcg ccatcttatt gccccgcgac aggtttcagc tcgatacgct
    15421 tgatatcgcc caccagcacc aggtagctca gtaccgcgat tagggcatga acaccaacat
    15481 aaatcagcgc cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg
    15541 tgacgatgcc agaaatgtta ccgaacatgt tgaactggcc accggaaaga ccgctgatct
    15601 cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga
    15661 aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca
    15721 tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc
    15781 ccgtgcggcg catcagccag tcggaaataa ttccacccag cacaccgccg ataaaaccgc
    15841 aaaccgccgg aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca
    15901 ccagataaac cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac
    15961 cgatataaac gccgatcatc atccgtgacc cgagcagctg tttgatctgc ccccacttca
    16021 cgctgaacgg cactttaact ttggtgcttt gctgatccat attgatcagc gcgccacccg
    16081 cggcgatata ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga
    16141 ctttcaacca gataaagctg atgacaatgc ccagaccgcc cataaagaag aagacgtgtg
    16201 accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt
    16261 attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg
    16321 caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca
    16381 gagtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca
    16441 aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat
    16501 ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca
    16561 catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag
    16621 aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc
    16681 agtaacgagc attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg
    16741 ttgcactcct gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa
    16801 tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg
    16861 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    16921 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    16981 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga
    17041 gtatgaaatg gatcgcttga ctctaggcaa acgccagtaa aaatccgcgc tatgaagcag
    17101 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc
    17161 tcgctgacat atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt
    17221 tccggttccg ggttctcact ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc
    17281 tgttcctgca acaaggcatc gaactgtttg ccgccattgc aaaaatttac ataactgctg
    17341 tcgccgagcg caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt
    17401 ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc
    17461 accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc
    17521 gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa
    17581 tttccgtaca tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt
    17641 aggaacttat ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag
    17701 gaagcagacc gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg
    17761 cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta
    17821 gcattaaccg ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatctccat
    17881 gtttgctatc gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt
    17941 tgcgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg
    18001 tcgctaccgg catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgggc
    18061 ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat
    18121 ccaacaccgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt
    18181 ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca
    18241 acacaccaga agtgggtcgg tccagacgat gaacagtaaa aacatgctgt cctatctggt
    18301 cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagcta cggtgaacca
    18361 gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca
    18421 ttagctcgca tcatccgcaa aaagagcatc cagtttttcc aactcagcca gaataagcgc
    18481 gcgttgcgga tggtccgtcg ctagcgccat ttcataatag ggtgcaacag caaaagcgcc
    18541 cggtaacggc tggttgttat ttaacaaatc gtgcattcgc gggatcagcc cccactgcaa
    18601 ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg
    18661 ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa
    18721 ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg
    18781 aaggatagca ggaataaaaa aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc
    18841 agcattccag ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg
    18901 cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc
    18961 atcctgaagt gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc
    19021 catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct
    19081 ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc
    19141 gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg
    19201 cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt
    19261 ttgattttaa atgaaaataa cgattcttat ctttttaagt attcgcttat ttcctggcga
    19321 tctcctacag agtctgtaag agatctctca caaccagatg gggtatttgg attacagaag
    19381 aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcatcc gtttacgtgt
    19441 tcccagcgtc tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc
    19501 ttcttccagc gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag
    19561 atgaccaatc agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa
    19621 tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa
    19681 ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg
    19741 ttccccggta aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca
    19801 cggtgaagga acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca
    19861 cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa
    19921 tttctctctt cacttaaacc agatacactt gtcgcttagt ttatctggtt tatgacggtg
    19981 aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac
    20041 cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg
    20101 cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct
    20161 gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga
    20221 acttgattac accagcgtaa atctgattga gtcgaagagt tttaagctct atctcaacag
    20281 ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt
    20341 aagcacttgc gctcagggta agattagcgt ggcgttatat cgtcttgatg aactggaagg
    20401 ccagccgata ggtcatttca atggcacttg cattgatgac caggatatca ctatcgataa
    20461 ctatgaattc actactgact atctggagaa tgccaccagc ggtgaaaaag tagtggaaga
    20521 gacgctcgtc agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg
    20581 ttcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct
    20641 ggtctcattc cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga
    20701 cctgttacgc ttctgccagc cagagaaatt gagcgtttac gcacgttata cccgtcgtgg
    20761 cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact
    20821 ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa
    20881 ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt
    20941 tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt
    21001 ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact
    21061 tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga
    21121 aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc
    21181 cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc
    21241 ggttctgcga gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa
    21301 cctgaatctc gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc
    21361 tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat
    21421 taacgaaaac gagtatttgt atgcccgtcg cgtcgggaac cagctgggcc tgcgtgagct
    21481 gaatatctgc accggctgtg gtccaggagc gatggaagcg ccgatgaaag gtgctgcggt
    21541 cggacacgcg cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat
    21601 tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga
    21661 aaaacgtctg gaagcgtttg tccgtatcgc tcacggcatc attatcttcc ctggcggtgt
    21721 gggtacggca gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga
    21781 tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact
    21841 ggacgagttt gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat
    21901 tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa
    21961 tcgccgtgat acaggcgatg cctacagctt taactggtca atgcgcattg cgccagattt
    22021 gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca
    22081 acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa
    22141 cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttacaaaa tcaacggcga
    22201 taaagagatt atgcgtcgta tggacgacct gctacagggt tttgttgccc agcatcgtat
    22261 gaagttgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctcccctcc
//
LOCUS       DAJTYF010000061        21834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTYF010000061 DAJTYF010000000
VERSION     DAJTYF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.061"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..161
                     /locus_tag="OO590_002396"
     CDS             <1..161
                     /locus_tag="OO590_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6637733.1"
                     /translation="TLSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNS
                     STAELLLK"
     gene            231..1037
                     /locus_tag="OO590_002397"
     CDS             231..1037
                     /locus_tag="OO590_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6637734.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1047..1454
                     /locus_tag="OO590_002398"
     CDS             1047..1454
                     /locus_tag="OO590_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6637735.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1454..1948
                     /locus_tag="OO590_002399"
     CDS             1454..1948
                     /locus_tag="OO590_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6637736.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2014..2811
                     /locus_tag="OO590_002400"
     CDS             2014..2811
                     /locus_tag="OO590_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6637737.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2822..3610
                     /locus_tag="OO590_002401"
     CDS             2822..3610
                     /locus_tag="OO590_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6637738.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3662..4891
                     /gene="sorE"
                     /locus_tag="OO590_002402"
     CDS             3662..4891
                     /gene="sorE"
                     /locus_tag="OO590_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6637739.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMELSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5273..5962
                     /gene="pepE"
                     /locus_tag="OO590_002403"
     CDS             5273..5962
                     /gene="pepE"
                     /locus_tag="OO590_002403"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6637740.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6053..7684)
                     /locus_tag="OO590_002404"
     CDS             complement(6053..7684)
                     /locus_tag="OO590_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6637741.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7904..11587)
                     /gene="metH"
                     /locus_tag="OO590_002405"
     CDS             complement(7904..11587)
                     /gene="metH"
                     /locus_tag="OO590_002405"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6637742.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISSDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTMPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11787..12611
                     /gene="iclR"
                     /locus_tag="OO590_002406"
     CDS             11787..12611
                     /gene="iclR"
                     /locus_tag="OO590_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6637743.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12928..15112
                     /gene="espL4"
                     /locus_tag="OO590_002407"
                     /pseudo
     CDS             12928..15112
                     /gene="espL4"
                     /locus_tag="OO590_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(15081..16817)
                     /gene="aceK"
                     /locus_tag="OO590_002408"
     CDS             complement(15081..16817)
                     /gene="aceK"
                     /locus_tag="OO590_002408"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR6637744.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(17001..18305)
                     /gene="aceA"
                     /locus_tag="OO590_002409"
     CDS             complement(17001..18305)
                     /gene="aceA"
                     /locus_tag="OO590_002409"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR6637745.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGESMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18335..19936)
                     /gene="aceB"
                     /locus_tag="OO590_002410"
     CDS             complement(18335..19936)
                     /gene="aceB"
                     /locus_tag="OO590_002410"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR6637746.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(20205..21134)
                     /gene="metA"
                     /locus_tag="OO590_002411"
     CDS             complement(20205..21134)
                     /gene="metA"
                     /locus_tag="OO590_002411"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6637747.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21291..21731
                     /locus_tag="OO590_002412"
                     /pseudo
     CDS             21291..21731
                     /locus_tag="OO590_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         5383 a         5591 c         5526 g         5334 t
ORIGIN      
        1 aaacgctctc tatcgaaatg aataaattaa agcaggcgcg atattccatt ggcattgcca
       61 tgagtgaaga aaaatacagc ggaattattg gtgcactgcg tggaaaatat attaattgtc
      121 tggtaacgaa tagcagcaca gctgaactat tactgaaata aaaatcttcc cgatgtttac
      181 cgtaataaaa attacggcgg gtccctatta tctaaaatca ggagtctgtt atgcaaacgt
      241 ggttaaattt gcaggataaa atcattattg tcaccggcgg cgcatccggt attggtctgg
      301 cgattgtaga ggaattatta gcacaaggcg cgaatgtaca gatggtcgat attcacggtg
      361 gcgatggtca atatgaaggt cataaaggct atcagttttg gccgaccgat atttctagca
      421 caaaagaggt aaatcatacg gtagcggaaa ttatccagcg ttttggtcgc atcgacggtc
      481 tggtcaataa cgccggggtc aatttcccgc gtctgctggt cgatgagaaa gcgcctgccg
      541 ggcagtatga actcaacgaa gctgcattcg aaaaaatggt caatatcaac cagaaaggtg
      601 tttttctgat gtcgcaggcg gtggcgcgac agatggtcaa acaacatgat ggcgtgattg
      661 tgaatgtttc ctcagaaagt gggctggaag gctcagaagg acaaagctgt tacgccgcta
      721 ccaaagccgc gctcaatagc ttcacgcgct cctggtcgaa agagctgggt aaacacggta
      781 tccgtgtggt cggtatcgcg cccgggattc tggaaaaaac cgggctacgc acgccggaat
      841 atgaagaggc gctggcgtgg acgcgcaata tcaccgtcga gcagctgcgt gaaggctata
      901 ccaaaaacgc cattcctatt gggcgcgccg gaagattagc agaagtagct gattttgttt
      961 gttatctgtt gtctgaacgc gccagctaca tcaccggagt aaccactaac attgcgggcg
     1021 gcaaaacgcg cggctaagga ggctttatgg tcaacgccat tttttgtgcc cacggcaaat
     1081 tagcttgcgc catgctggaa tcggttcaga tggtttacgg caacgccaat gttgaagccg
     1141 ttgaatttgt gcccggtgaa aacgccggag acattgcggt aaagctggaa aagttagcaa
     1201 gcattcacaa tcaagatgag tggctgattg ccgtggattt acagtgcggc agcccgtgga
     1261 atgcggcagc aatgcttgcg atgcgcaatc ctcgtttgcg ggtaatcagc ggcctttctc
     1321 tgccgctggc gctcgaactg gttgataacc aggacagcat gaatgttgat gaactgtgtg
     1381 aacacctgac gcaaatcgca aagcaaacct gcgttgtctg gaaacaactg gcaacgacag
     1441 aggaggattt ctgatgaaca ttacgctcgc ccgcattgat gaccgtctga tccacggcca
     1501 ggtcactacc gtctggtcga aagtggctaa cgcccagcgc attattatct gcaatgacga
     1561 agtttataac gatgaagtgc gccggacatt attgcgtcag gctgcgccac cgggaatgaa
     1621 ggttaacgtg gtcaatattg aaaaagccgt cgcggtttat cacaacccgc aatatcagga
     1681 cgaaaccgtt ttttatcttt tcactcgccc acaggatgcc ttagcgatgg ttcgccaggg
     1741 ggtaaaaata ggcaccctga atattggcgg catggcctgg cggcccggta aaaaacagtt
     1801 aaccaaagcg gtttcattag atgatgatga tattaacgca tttcatgagt taaataatct
     1861 tggtgtcata ctggatttac gcgtcgtagc gtcagatccc tcaatcaata ttatcgacaa
     1921 aataaacgaa cagttaatcg caaattaaat aatagcgcct gtgtacatat gctttaacag
     1981 gcagggatga cgacacttaa aaggtgacaa atcatggaaa taagtaccct acaaatcatt
     2041 gctatatttc ttttttcctg tattgccgga atgggcagcg tgctggatga atttcagact
     2101 catcgcccgt taattgcctg tacggtgatt ggtttaattc tcggtgattt aaaaaccgga
     2161 ataatactcg gcggtacgct ggaattgata gctctcggct ggatgaacgt cggcgcggcg
     2221 caatctccgg attctgcact cgccagcata atctccgcca ttctggttat cgttggtcag
     2281 caaagcatcg ccaccggaat cgccatcgcg ttgcctgtgg ctgcggcagg ccaggtgctg
     2341 acggtgtttg cccgtaccat caccgtagtg ttccagcacg cggcggataa agcagcagaa
     2401 gaggcgcggt ttcgcactct cgatattctg catgtctccg cgcttggcgt gcaggcgctg
     2461 cgcgtcgcca ttccggcact gattgtctca ctgttcgtca gcgccgatat ggtcagcaat
     2521 atgttaaacg ccattcccga atttgtgacc cgaggactac agattgctgg cggttttatc
     2581 gtggtggtcg gttacgccat ggtgctacgt atgatgggcg tgaaatattt gatgcctttc
     2641 tttttcctcg gtttcctcgc aggtggctac ctcgatctca gcctgctggc gttcggtggc
     2701 gtcggcgtga tcatggccct ggtctacatc cagctaaatc cacagtggcg taaagctgaa
     2761 cctcaactcc aggccaccgc ctccaccgcc cttgaccaac ttgacgatta acggagccat
     2821 catggaacag agaaaaatta cacgcagcga tctggtgagc atgtttctgc gctccaacct
     2881 gcaacaggcg tcctttaact ttgaacgtat tcacgggctg ggcttttgct acgacatgat
     2941 ccccgccatc aagcgacttt acccattaaa agaggatcag gttgcggcgc tcaggcgaca
     3001 cctggtgttc ttcaatacca cgccagccgt atgtggcccg gtcatcggcg cggaaattga
     3061 tgacggtacc atcaacggca tcaaagtcgg tctgatggga ccattggcag gagttggcga
     3121 tccactggtc tggggaacgc tgcgcccgat taccgccgcg ctcggcgcat ctctggcact
     3181 ttcgggcaac attctcggcc cgctgctgtt cttctttatt ttcaacgcgg tgcgtctggc
     3241 gatgaagtgg tatggcctac agctcggctt tcgcaaaggg gtgaatatcg tcagcgatat
     3301 gggcgggaat gtgctgcaaa aactcaccga aggcgcgtcg attctcggac tgtttgtgat
     3361 gggcgtgctg gtgaccaaat ggacgtcaat caacgtaccg ttggtggttt cacaaacgca
     3421 tgccgccgat ggctccaccg tcaccatgac cgtgcagaac attctcgacc aactttgccc
     3481 tggtttgctg gcgctcggtc tgacgctact aatggttcgt ctgctcaaca aaaaaattaa
     3541 cccggtatgg ctgattttcg ccctgtttgg cttagggatt atcggcaatg ctctgggctt
     3601 cctgtcctga ttcttcgccc cggcacgact gccgaggcca tcgctcaaca tgaggtggtt
     3661 tatgaaaaca acagctctgc gtctttatgg taaacgtgat ttacgcctgg aaacctttga
     3721 ccttcctgaa atgcaggagg atgaaatcct cgcaacggtg gtcactgaca gcctgtgcct
     3781 ctcttcctgg aaagaggcca atctgggtga aaaccataaa aaagtacccg acgatgtggc
     3841 gaccaaccca atcatcatcg gccacgaatt ttgcggcgat attctggccg tgggtaaaaa
     3901 gtggcagcac aaattccagc cgggtcagcg ttatgtgatt caggccaacc tgcaactccc
     3961 cgaccgcccg gattgccccg gctactcctt cccgtgggta ggcggcgaag cgacgcatgt
     4021 ggttattccc aacgaggtca tggaacaaga ttgcctgctg gcatacgacg gcgaaaccta
     4081 ttttgaaggc tcactggttg aaccgctttc ctgcgtgatt ggcgcgttca acgccaacta
     4141 tcatcttcag gaaggtagtt ataaccacac gatggggatt cgcccgcaag ggcgcacgct
     4201 gatcctcggc ggcaccggac caatgggact gttggcgatt gattatgcgc tgcatggacc
     4261 agttaacccg tcgctgctcg tcattaccga taccgacaac gacaaattga gttatgcgcg
     4321 caagcactat ccatcagaac cgcaaacact gattcattat ctcaatgcca ccgatgcagc
     4381 atttgatacg ttaatggagc tgagtggcgg tcacggcttc gatgatattt tcgtctttgt
     4441 gcctaatgaa ggactggtga ctctcgcctc ttccttgctg gcgacagatg gttgcctgaa
     4501 tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca attaatttct acgatgtgca
     4561 ttatgcattt acccactacg tgggcacgtc aggcggcaat accgacgaca tgcgcgcggc
     4621 ggtcaaactg atcgaagaga aaaaagtgca ggccgcaaaa gtggtaacac atattcttgg
     4681 gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc gtcggcggcg gcaaaaagct
     4741 ggtgtatacc gggaaatacc tgccgctgac gtcactcacg cagattcagg atcaagcact
     4801 ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt gaggcggagc aatacctgct
     4861 cgcccatgca gaggcaattt cccatgatta atcgcgctac ttaactctgc atgtcgctgg
     4921 ctggtcgccc cagcaacttc gcgaccggat gtgcggctga ttgctgaagc agcacaattt
     4981 gcacgagaag gataaaaaat atcagcgcct taaggggcgc tcaaattggt ggcaaagtac
     5041 acattgttta tgccggatgc ggaatgaagc cttattcggt ctacataatt ttgaaaatcc
     5101 aatatattgc agttatctgg taggccaggt aagcgtagcg catcaggcaa tgctgtgttt
     5161 gtcatcagtc tcagcgcccg gtgcaatacg tgacatcgtg agccatgtca gtaagtggtt
     5221 aaattcgcag tggtagcatg ccttccgtga tttcttacta accggagaag taatggaact
     5281 gcttttattg agtaactcga cgctgccggg taaagcctgg ctggaacatg cactgccgct
     5341 aattgctgaa cagttgcagg gtcgccgctc agcggtgttt atccctttcg ctggcgtaac
     5401 gcagacctgg gatgattaca cagagaaaac ggctgcggtt ctcgctccgc tgggtgtttc
     5461 tgtcaccggt attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg cggaaattgt
     5521 gattgtcggc ggcggtaata ctttccagtt gctgaaacag tgccgcgagc gcgggctgct
     5581 ggctccaatt actgacgtgg ttaaacgtgg cgcgctgtat attggctgga gcgcaggcgc
     5641 taaccttgct tgcccaacca ttcgtaccac caacgatatg ccgattgtcg atccgcaagg
     5701 tttcgatgcg ctaaatctgt tcccgttgca aatcaacccg cacttcacca acgcgctgcc
     5761 ggaaggccat aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc
     5821 agaactgacg attattggtc tgccggaagg taactggatc acagtgagta aaggtcacgc
     5881 tacgctgggt ggcccgaaca ccacttatgt gtttaaggct ggtgaagaag cggttccgct
     5941 ggaagctggt caccgttttt aagtgaattg ccggatgcat gatccggcat cttgttcata
     6001 gtgcctgatg cgacgcttga cgcgtcttat caggcctaca ggattcacaa tattaatact
     6061 cgtcccgtcc ttcatcttca tccggctgtt ccagcacact gtaagccacc gaacaaaaca
     6121 gcgagttcag gcgctgcata tctcccagta agcctaaatg tagcgaactg gtttcaatgc
     6181 tctgcacgtt ttgctgatgc aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa
     6241 tgcgaaaacg atgtttgctg cgacgcaaac gacgagcgct ggtgacatcg ccagagaaga
     6301 acaccgacat tgccagcttt aaattactga gcaattgctc atagagcgca tccagttcct
     6361 tcaacccatc aagcgaaaat gcccgccgtg ctgccagcga tttatcggca atttcgctgc
     6421 ccatgcgctc gacgatatcg gaggcctgtt caaggttgag cgacatttcg atgatctccg
     6481 cccagcggcg cgactcttcc tccgccaact cttcttttgg catccgcgcc agatacagct
     6541 taatggcggt gtagagaacg ttgatatcat ctgccagctt acgcagctct ttctcctgcc
     6601 gtggctcgcc gtgcatcact ttattcagcc cttccatcat ctgttccatg gcgtcgccaa
     6661 tgcgcagggt ttcgcgcgcg gcgttcgcca gagcaagcgt gggcgtgtcc agcgcgctga
     6721 catccagatg tttaggccgt agctgggtat ccagttccgg ttcatcgcga ataatcgttt
     6781 tgcaaaaccg tgccatcggg tcaacaaatg gcagcatgac caggcaacgt acaaggttgt
     6841 agaagacgtg gaaatagatc accagttccg cttttggcaa tgacagcttc cccattgtct
     6901 ctgccagcaa atggacaaac ggcaggataa tcaggctacc caccagctta aacagcagac
     6961 tacccagcgc gacacggcgg gctgcggcat tggcggcact gttgttgagc atcgccagca
     7021 ggccggaacc gaggttagct ccaatcacca gacagagcgc cacggggaag gagataatgc
     7081 ctgcagcggt cagagtcgcg gtcagcagta cagcagcaag gctggagtag ctgataatgg
     7141 cgaacatcgc gccaatcagc gcatccagca gaatatcgcc ggtcagcgag gcaaagatca
     7201 cctgaacgcc gtttgcctgg gtgatcggcg ttacggcctg cacaatcaac tccagcgcca
     7261 gcaaaatcag cccaagacca ataccgacgc ggcccagttg cccggcgcgt gactgtttgc
     7321 gtccgaggaa aaaaatcacg ccgataaaaa taagtaacgg tgacagccag gataagtcga
     7381 aggtgagaat acgcgccatt agcgccgtcc cgacatctgc ccccagcaca atgaccagag
     7441 ccggtgcgag ggctaccaga tcctgagcga cgaacgaggt caccagcatg gtggtggcat
     7501 tactgctctg taccagtgcg gtaacgccga tccccgcgca aaaggcgagc ggcttttttt
     7561 cgacgctccg gctaaggaca gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc
     7621 gaacaatatg agtcccccag accagcaggg cgacggcaga aagcaggtga agcagcgtta
     7681 acacgagttc aggttctcct tatcgttata tgttcctgaa gcaattgaat gaccaaagga
     7741 ccggaaacac gctgactggc atctttactg tgccttttga cattcgtttt tccggctaag
     7801 attattccag cctgtttcca gtataagtgt ttcatattaa taaagagaca gggcactcat
     7861 tgagcgccct gtttgttgta aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc
     7921 ccagattcgg tgccagccag cgctcaacat cggagacgct cataccttta cggcgggcat
     7981 agtcttcaac ctgatcgcgc tgaatttgcg ccaccgcgta gtacttgctg tccggatgac
     8041 tgaaatacca acccgaaacc gatgcaccag gccacatagc gaaagattct gtgagtttca
     8101 tgccagtatg tttttccact tccagcagct cccagatggt ggctttttcc gtatgttccg
     8161 ggcaggccgg atagcccggt gccggacgga tgccctggta gttttcgcgg atcagttctt
     8221 cgttgctgag gttctcgttc ggcgcatagc cccagtagac tttacgcaca cgctcatgga
     8281 gatactccgc aaaggcttcg gctaaacggt cggcaagcgc tttcaccatg attttgttgt
     8341 aatcatcgtg ctgcgcttca aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca
     8401 cggcaaatgc gccgatgtaa tctgctttac cagaaagctt cggcgcaacg aagtcagcga
     8461 gacagtagtt agcgaatccc gttttttcgg tctgttgacg cagatggtgg ctgacgttga
     8521 tcacatgggt gcgcgtttcg tcacggtaga tttcaatgtc atcgcccaca cggtttgccg
     8581 ggaacaggcc caccacgcca cgcggattca gcgttttctc ggcgcttaat ttatccagca
     8641 tgtcgttggc gtctttaaac agccgctgcg cctcaacgcc caccacttca tcttccagaa
     8701 tgcgcggata cttcccggcc agcgaccagg tcataaagaa cggtgtccag tcgatgtaat
     8761 tacgcagcgt ttcgatgctg gcttccactt cctgcacgcc gagacggtgc gccaccggcg
     8821 gcgtgtaagc ctgccagtca aatgcgaaat cgttatcgcg cgccgcttcc agcgtgaccg
     8881 gtggtgtgcg tggtttcttg cgcccgtgct gaatacgcac ggtttcgtac tccttgcagg
     8941 tacgagcgac aaaatcatca cgctgagtat cggaaagcag cgccgctacc acaccaacgg
     9001 tgcgcgaggc attctgcaca tacaccgtag ggccgctgta gttctgctcg attttcaccg
     9061 ccgtgtgcgc ttttgaggtg gtcgcgccgc caatcagtaa cggaatggtg aagccctgac
     9121 gctccatctc tttcgccacg ttaaccatct cgtccagcga cggcgtgata agccccgaaa
     9181 ggccaatcaa atcagcattc acttctttgg cgatacggag aattttttcc gcaggcacca
     9241 taacgccgag atcgacaatt tcgtagttgt tacattgcag caccacacca acgatatttt
     9301 taccgatgtc gtggacatcg cctttcacgg tggcgatcac catcttgccg ttggttttac
     9361 cctgctcttt gctggcttca ataaacggtt cgagatacgc cactgcctgt ttcatgacgc
     9421 gcgccgattt gaccacctgt ggcaggaaca ttttgccttc gccaaacaga tcgccgacca
     9481 cgttcatgcc gtccatcaac gggccttcaa tcacttcaat cgggcgcgta gcctgctggc
     9541 gggcttcttc agtatcctgc tcgataaact cggttatgcc tttgaccagc gagtattcca
     9601 gacgtttatt cacgtcccac gagcgccact ccgcttgctg ggcgttagca gtgtcgtccg
     9661 ctttactgcc gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca tcgtcacgac
     9721 gattaagaat cacatcttcc accgcgtcgc gcagttcagc gggtaggtcg tcgtaaatcg
     9781 ccagttgccc ggcgttgacg atccccatat ccatgccatt gcgaatagcg tagtagagga
     9841 acactgcgtg aatggcttcg cgcaccggat cgttgccacg gaatgagaaa gaaacgttag
     9901 atacgccgcc ggaaatcagc gcgtgcggca gttcgcgttt gatgtcttca cacgcgccga
     9961 taaagtcctg cgcgtagttg ttgtgctctt caataccggt ggcgaccgcg aagatgttcg
    10021 ggtcgaagat gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc
    10081 gacggcaaat ctcgattttc cgtgcgcgag tatcggcctg tccctgttcg tcaaaggcca
    10141 tcaccaccac tgccgcaccg tagcgacgca acaatttcgc gtgatggata aaggcgtcga
    10201 cgccctcttt catcgagata gagttaacaa tgcctttgcc ctggatacac ttcagacctt
    10261 tttcaatgac atcccatttt gaggagtcga tcataatcgg cacgcgagcg atatccggtt
    10321 caccggcaat cagattgaga aaacgcacca tcgccgcttc ggcatcgagc atcccttcat
    10381 ccatgttgat atcgataatc tgcgcgccgt tttccacctg ctgacgcgcc acatccagcg
    10441 cctcgctgta tttctcttct ttgatcaggc gcttgaactt agcagagccg gtgacgttgg
    10501 tgcgttcacc cacgttcaca aacaggctat catcgccaat gttcagcggc tccaggccag
    10561 acaaacggca ggctacggga atttccggca gtttgcgcgg cgctaatcct tctactgcac
    10621 gactcatcgc tgcaatatgt tgcggcatgg tcccgcagca accaccgacg atattgagaa
    10681 agcccgcttg cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtatt
    10741 caccaaaggc gttaggcagc ccggcgttcg ggtgggcggt gacgtagcat tccgcaatcc
    10801 gtgatagttc ctgcacgtac tggcgcagtt catcgggccc cagcgcacag ttcaggccaa
    10861 aggtcagcgc ttcggcgtgg cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga
    10921 gcgtgcgccc ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc tcaacgccca
    10981 gcgcttcaaa ctccgttttc accgcaaaga ccgccgcttt ggcgttaagg gtgtcgaaaa
    11041 cggtttcaat caggatcaga tccgcaccac cttccaccag cgctttggtg gattcgcgat
    11101 aggccgccac cagctggtca aaagtgatgt tgcggaatgc cggatcgttg acgtccgaag
    11161 aaatagacgc cgtgcggttg gttgggccga gaacaccggc aacgtagcgc ggtttctccg
    11221 gcgtgcgcgc ggtccactcg tcagcacggg cgcgcgccag tttcgccgcc gcaaagttga
    11281 tttccgccga cagggattcc atctggtaat ccaccatcgc aatggtcgtg gagttgaagg
    11341 tgttggtttc gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca
    11401 cttccggttt actgagtacc agcaggtcgt tgttgccttt gaggtcgcat ggccagtcgg
    11461 caaagcgttc accacgaaaa tcggcttcgt tcagtcgata gctctggatc atggtgccca
    11521 taccgccgtc caacaccaga atacgttcat ttaactgcgc acgcagttgt tccactttgc
    11581 tgctcacact tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac
    11641 tggcataaac gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt
    11701 aactcatcgg atcagttcag caactattgc attagctaac aataaaaatg aaaataattt
    11761 ccatgataca gaaaaaggag gctgtcatgg tcgcccccat tcccgcgaaa cgcggcagaa
    11821 aacccgccgt tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga
    11881 aattactgga gtggattgcc gaatccaatg gcagcgtggc actcacagag ctggcgcaac
    11941 aagccgggtt acccaattcc acgacccacc gcctgctaac cacgatgcaa cagcagggtt
    12001 tcgtgcgtca ggtcggcgaa ctgggacact gggcaatcgg cgcacatgcc tttatggtcg
    12061 gcagcagctt tctccagagc cgtaatttgt tagcaattgt tcaccctatc ctgcgcaatc
    12121 taatggaaga gtctggcgaa acggtcaata tggcggtgct tgatcaaagc gatcacgaag
    12181 cgattattat cgaccaggta cagtgtaccc acctgatgcg aatgtccgcg cctatcggcg
    12241 gtaaattgcc gatgcacgct tccggtgcgg gtaaagcctt tttagcccaa ctgagcgaag
    12301 aacaggtgac taagctgctg caccgcaaag ggttacatgc ctatacccac gcaactctgg
    12361 tgtctcctgt gcatttaaaa gaagatctcg cccaaacgcg caaacggggt tattcatttg
    12421 acgatgagga acatgcgctg gggctacgtt gccttgcagc gtgtattttc gatgagcacc
    12481 gcgaaccgtt tgccgcaatt tctatttccg gaccgatttc acgtattacc gatgatcgcg
    12541 tgaccgagtt tggcgcgatg gtgattaaag cggcgaagga agtgacgctg gcgtacggtg
    12601 gaatgcgctg actttttctg gcgggcagag gcaatattct gcccatcata cctgagtggc
    12661 aatagaataa gggtgtctgt taatcgcatt gacgccaaaa taacttaatg tcatacactt
    12721 cacacacttt tattaactca gcattatttt taaacatcaa accacttaat tattaacaaa
    12781 atcgcaagat ctcattccac cgaaactttt acggctgcat tatgagattg agcgatttat
    12841 gatatttttt tagttgtaat catattaaat tttttcactg ttctgatagt taaaattcaa
    12901 gacatcaata aacaatgaga tatttaaatg attactcgta ttcctcgtag ttctttctct
    12961 gcaaatatta ataatacagc ccagacaaat gaacaccaaa ccctgagtga attgttttat
    13021 aaagaactcg aggataaatt ttctggcaag gagctggcga cgcctctatt aaaaagcttc
    13081 tcagagaact gtagacaaaa tgttcgtcat atctttagca acaaggattt tgtcattaaa
    13141 ttttccacgt ccgtcttaca agctgataag aaagaaatta cgataattaa taaaaacgaa
    13201 aacacgacac tcactcaaac cattgctaca atatttgaag aatacctaat tgaaatttta
    13261 cctcaacgct cagacactct tgataaacaa gaattaaacc taaaatcaga tagaaaagaa
    13321 aaagaattcc caagaattaa acttaatggt caatgttatt ttccggggcg ccccaaaacc
    13381 gtatagtata ccgacacatt gctgcacaat atattaatga tatttatcag aatgttgatt
    13441 acaaacccca tcaagatgat tactcttcag ctgaaaaatt tctcactcac ttcaacaaaa
    13501 aatgcaaaaa ccagactttg gcgttgattt ccagccgtcc tgaggggcgt tgcgttgctg
    13561 cctgcggtga tttcgggcta gttatgaaag catattttga caagatggaa tcaaatggca
    13621 tcagtgttat ggcagccata ttactggtgg ataaccatgc tttgacggtc cggctaagaa
    13681 taaagaacac aactgaagga tgtacccatt acgtgatttc ggtttatgat cctaatgtaa
    13741 ctaacgataa aataagaatt atgagcgaaa gcaaagagga tattaaacac tattctctga
    13801 tggattttat gaatgtagat tatagcctcc tgaaatggtc aaatgatcat gttattaacc
    13861 aatctgttgc aataattcca gcacttccga aagaacagct attgatgtta aaaggaactg
    13921 tggatgaaat aacccctcca ttatcaccag caacgatgaa tttgctaatg gcaattggtc
    13981 agaatcacca acttacgcaa ctgatgattc agctccagaa aatgccagaa ctacatagaa
    14041 cagaaatgtt gactgcctat aatagtatta acctccccgg tttatatttg gctataaatt
    14101 atggcaatgc ggatatcgtt gagactattt tcaattcatt gtcagaaaca ggatatgaag
    14161 ggttactctc gaaaaaaaat ctcatgcata ttctggaggc aaaagataaa aatggttttt
    14221 ctggattatt tttagcgata tcacgtaagg ataaaaatgt tgtaacctcg attctgaacg
    14281 tcttacctaa actggccgca acacatcatt tagataatga acaggtgtat aaattcctga
    14341 gtgccaaaaa tagaacgtcc agccatgttt tataccatgt tatggcgaat ggtgatgccg
    14401 acatgctgaa aattgttttg gtcgcgttac ctttgttaat tcgcacatgt catttgacta
    14461 aagaacaggt actcgatctc ctgaaggcaa aggattttta tggttgccca agactatacc
    14521 tggcgatgca aaatggacat agctatatcg tgaaagttat tctcgaagca ttgccctgtc
    14581 tggcccagga aattaacatt tcagcttccg atattgtaga tcttctgacc gctaaaagtc
    14641 ttgcgcgcga cacgggtttg tttatggcca tgcagcgcgg acatatgaac gttattaata
    14701 ctatttttaa cgcattaccc actcttttta atacgtttaa attcgataaa aaaatatgaa
    14761 gcccctcctc ctggcaaata attctaatga atatcccggt ttgttttcag cgatacagca
    14821 taaacaacaa aacgttgtag agacggttta tcttgcttta tctgaccatg cacgcctgtt
    14881 tggatttacc gctgaagata ttatggattt ttggcaacac aaagcgccac aaaaatactc
    14941 tgcctttgag ttggcttttg aatttggtca ccgggttatt gctgaattaa tccttaatac
    15001 attaaataag atggctgaaa gctttggctt tacggataac cctcgataca ttgcggagaa
    15061 aaattatatg gaagctttac tcaaaaaagc atctccccat accgtacgct aaatctttgc
    15121 ctgcgccgat acgcataaac atcttccaca tgcccttcac gtatgcggtt ttgtagtgcg
    15181 cgccagtaat cagcgcggaa caggtcggcg tgcatctctt caaacagcgg accaatacgc
    15241 gggtcggcgc atagccagtg gcgaaactct tccgggaaaa catcgcccgg cgagacgctg
    15301 taccacggtt cgctggcaag ttcgtcttcc ggatagcgcg gcggcgggat gtcgcggaaa
    15361 ttcacttccg tcatgtagca aatttcatcg taatcataaa aaaccacacg cccgtgacgg
    15421 gtgacaccga agtttttaaa cagcatgtcg ccagggaaaa tgttagcagc ggcaagctgg
    15481 cgaatagcgt taccgtattc ttcaatggcg tcgcgcaact gctgaccttc cacttgttcc
    15541 agccagatat tgagcggcac catccgccgc tcgatataga gatggcgaat tacaatctgc
    15601 tcaccaagat cggtgatttt ttccgctgct tcctggagca gtaattccat taataccggg
    15661 gaaatatgcc gcttctccag cacaaagttt tcaaactcct gggtgtccgc cattcggccc
    15721 acacggtcgt gctctttcac cagttgatag caggcacgaa cgtgagcggc agacatctct
    15781 ttctgcggcg cgaacttgtc tttgatgact ttgaataccc gatcaaagcc cggcagcgta
    15841 aacaccaaca tcaccattcc acgaataccc ggcgcttcaa tgaactgctc attacagccc
    15901 tgtagataaa cgagatattc gcggtagctt tcggttttgg cgtgcttctg gcagccgata
    15961 gccatataca attcagcagt ggttttacct ggcagaattt cccgtagcca ctcgaccagc
    16021 gctgcgggca gcggtgcata aaccataaaa taagaacgcg caaagccaaa aacaatgctc
    16081 gcttcggcgg tcgtcgtcag gcaggtatca ataaataact cgccgtcgtc cgtctggtgg
    16141 atcggcagta agaagggcaa cgtgccggaa ggcgtgatca gtttgcccac cagccaggcg
    16201 gctttattgc ggtaaaacag ttcgttcgcc acctgtaaat gactttccgc gaggttgtct
    16261 gtccccagcg tttccgtcag atggcgaatg atgtaatgaa tgtcacggct tttattctgc
    16321 cagcgcaggc gcagcggcag gtcgctgata acgcgcatca gtagagattc ccagccgtga
    16381 tcggggtgaa agtctttcgc cagcgggcgg ggaatggtac gaaagcggcg ctctggctga
    16441 gagctaaaga taaaaagccg ctcgggagta agcgagcggt ggtcaaataa ccgacagtac
    16501 acggagttaa aaaagctctc cgcaatctcg aagcgcgggt aatccggcaa cagccgcgtg
    16561 taatgctctt taacgcgcag taaaaatgcc gcgtccgtac tttggccgtt agtaatgcag
    16621 cgcagttgct ccacgaccag accaacgtga tgatcgtaaa gatggatacg gtttttcatc
    16681 gcctgctgga cagcatgcca gtcggcctgt tcgaaacgct gctgcgcccc ggaagtcact
    16741 tcgaggaatc gaccatactg agcgtcgaag ccttgcaaaa tggtttgagc aatcagtaat
    16801 tccaggccac gcggcattta gcgcactcat caggagcaga gaattgccgg atgcggcgta
    16861 aacaccttat ccagcctacg ttcggcaacg gttgtaggca tgataagacg cgcaagcgtc
    16921 gcatcaggca tcgtgcaccg attgccggat gcggcgtaaa tgccttatcc ggcctacagt
    16981 cagcaacgtt tgtaggttgc ttagaactgc gattcttcag tggagccagt cagcgcggtg
    17041 actgaagacg tgccgccctg aataatagtc gtcactttat cgaagtaacc tgtacccact
    17101 tcctgctggt gagatacgaa ggtatagcca tctttcgcgg cggcaaattc cggctgctgc
    17161 actttctcaa catagtgctt catactttcg ccctgcgcat aagcgtttgc caggtcgaac
    17221 atgttaaacc acatgctgtg gatacctgcc agggtgatga actggaactt gtagcccata
    17281 tccgacaact gctgctggaa gctggcaata gttttgtcgt cgaggttttt ctgccagttg
    17341 aacgacggcg agcagttata agccagcagt ttgcccggat atttcgcgtg gatagcttgt
    17401 gcaaagcgac gcgccagttc cagatccggc gtggaggttt cacaccagac caggtcagca
    17461 tatggcgcat acgccaggcc acggctgatc gcttgctcaa tgcccgcatg agtacggaag
    17521 aagccttcac tggtacgctc gccggtaata aattcgctgt catacgggtc gcaatctgag
    17581 gtgatcagat ccgccgcatc agcatcggta cgtgcaacca gcagggttgg aacgcccgtc
    17641 acgtcagctg ccagacgcgc cgcgaccagt ttctgaatag cttcctgagt tggcactaaa
    17701 actttgccgc ccatgtgacc gcatttcttc actgacgcca gctgatcttc gaagtgaact
    17761 gccgcagcac cggcttcaat catcgctttc atcagttcaa aggcattcag gacaccgcca
    17821 aaaccggctt ccgcatcggc aacgatcggc aggaagtaat cgacatagcg cggatcgccc
    17881 ggctcaatgc ccacggacca ttggatctga tcggcacgac ggaaggtgtt gttgatccgc
    17941 tccaccacag ctggcaccga gtttgccgga tagagcgact gatccggata catgctggcc
    18001 gccaggttag cgtccgccgc tacctgccat cccgacagat agactgcttc aatacccgct
    18061 ttcgcctgtt gcagcgcctg accgccagtc agtgcaccga ggctgttgat gtagcctttt
    18121 ttcgactcac cgtgcagcag acgccacatt ttcgctgcgc ccagttgcgc cagcgtgcat
    18181 tcaggattga ctgaaccgcg taatttcacc acatcttccg cactgtatgg gcgagtaatg
    18241 ccttcccaac gcggttgagt ccactctttc tgtaattctt caatttgttg tgtacgggtt
    18301 ttcatgtgca gatgctccat agttatgtgg tggtttacgc taacaggcgg tagcctggca
    18361 gggtcaggaa atcaattaac tcatcggaag tggtgatctg ttccatcaag cgtgcggcat
    18421 cgtcaaaacg gccctgggag aaacgttctt cgcccagttc gctggcaatg actttcatct
    18481 cttcgcccag catctggcgg aacaaggctt tggtcaccgg tttgccattg ctcaacgttt
    18541 tttgatgatg gatccactgc cagatcgagg tacgggaaat ttcagccgtc gccgcatctt
    18601 ccatcaggcc ataaatcggc acgcagccgt tgccggagat ccacgcttcg atgtactgca
    18661 cagccacgcg aatgttggcg cgcatacctt cttcggtgcg ttcaccatca caaggtgcca
    18721 gcagctgatc ggcagtaatc ggcgcgtctt gttcgcgcaa tacttcaagc tgatttttac
    18781 gggagccgag aatatcgttg aataccgcca ttgccgtgtc cgcaaggcct gggtgagcga
    18841 tccatgtgcc atcgtgaccg ttattggctt ccagcgcttt atccgctttt actttgttga
    18901 gcacctggtt attacgctct tcatctttgc tcggaataaa cgctgccatg ccgcccatcg
    18961 caaaagcacc gcgtttatgg caggttttaa tcagcaggcg tgagtaagca ttcaggaatg
    19021 gtttatccat cgtcacggcc tgtctgtctg gcaggacacg atcgggatag tttttcaacg
    19081 ttttgatata gctgaagatg taatcccagc gaccgcagtt cagaccaaca atatggtcgc
    19141 gcagcgcgtg aaggatttcg tccatctgga acaccgcggg cagcgtttca atcagcaacg
    19201 tcgccttgat ggtgccgcgc ggcagattaa agcgatcttc tgcatagctg aagacttcgc
    19261 tccaccaggc cgcttcctgc caggactggg ttttcggcag atagaaatag ggaccgcagc
    19321 cctttgccaa cagtgcctga tagttgtgga agaaatagag cgcaaaatca aacaggctac
    19381 cggggattgc ctcaccacgc caggtgacat gtttttccgg caagtgcaga ccgcgtaccc
    19441 gacaaatcaa aaccgctgga ttgggcttga gctgataaat tttgcctgct tcattggtat
    19501 agctgatggt accgttaacc gcatcgcgca ggttaatttg cccgtcgatc actttgttcc
    19561 agtctggtgc cagtgaatct tcgaaatcgg ccataaagac tttcacattg gcgttcagtg
    19621 cgttgatcac catcttgcgc tctaccgggc cagttatctc tacgcggcgg tcttctaagt
    19681 ccgcaggaat cccgcgaatt ttccaatcag catcgcgaat ggaagccgtt tccgaaataa
    19741 aatcaggcaa cgttccgtta tcaatatctt gctgctgctg aatgcgcgct gccagaagtt
    19801 tattgcgttg tggcgtaaaa tgcgtcacca gctcagtcag aaattctacc gcttcggcag
    19861 taagaatttg cttctcctgc tcgccatacg gccttgtgaa agccagttca tcggttgttg
    19921 ttgcctgttc agtcatcgtg cagctcctcg tcatggatcc gaaaacttcc cctctgtgaa
    19981 cgaaggaatg ttgtgcgctt ttcgttcagc acaactaaga ctactcattt aaaatgcaaa
    20041 aacaaaaaca atttccattt taattaaaaa tacttgataa cttattgata acaatatgaa
    20101 taaaatttat tcactttctg aggtgcgttt cggaaataaa aaaggcaccc gaaggtgcct
    20161 gaggtaaggt gctgaatcgc ttaacgatcg actatcacag aagattaatc cagcgttgga
    20221 ttcatgtgcc gtagatcgta tggcgtgatc tgatagacgt aatagttgag ccagttggta
    20281 aacagcaaat taccgtgact acgccagctc gctcgcggtt tgttttgcgg atcgttgtgc
    20341 gggaaatagt tatacggtac atccgggtct agtccggctt ccacatcgcg gaaatattcc
    20401 tgcgccagcg tttgcgcatc atattcggga tggcccgtca caaaggcaat gcgcttatct
    20461 ttgctggcaa acagatatgc atccccttct tccgtctctg ccagaatttc cagatcggtg
    20521 taatcacgaa tcaacgctgc cggaaagtca gcatagcgcg aatgcggtgc caggaatgaa
    20581 tcatcaaagc cacgcgtcag aagcgcatga ggatggagaa tatgatgctc gtaaacgcca
    20641 gagagtttgt cggtgcgagt ttgcttagga atgccgtaga ggatattgag cgcggcctgt
    20701 accgcccagc agacaaacag cgtcgaggtg acgtgatctt tcgaccactc cagcacctgt
    20761 ttgatctgcg gccagtaagc gacatcatta aactccacca ggcccagcgg cgcaccggtt
    20821 acaatcagac cgtcaaagtt ctgttcctga atatcttcaa agttacagta gaagttgttc
    20881 agatgctctg cgggcgtgtt gcgcgattca cgggaatcga tgcgcaacag ctgaatatcg
    20941 acctgcaaag gtgagtttga aagcaggcgc agaaactgat tttcagtttc aatcttcttc
    21001 ggcatcaggt taaggatcag aaccttaagt ggacgaattt cctgaccaga cgcacgagaa
    21061 gttgtcatca caaacacgtt ttcttcacgc aagaaattga cggcgggtag ctcgtccggc
    21121 acacgaatcg gcataacctg attacctcac tacatacgct tatacgttta gacatccaga
    21181 tagctgaaga taaccagaaa atttgccaat gtcgagcctg catgttgaag gtgagaaagt
    21241 ttcaaggaaa aggctacgtt agaatataag aatgacgaaa aggagagaaa atggttatta
    21301 gtattcgccg ctcacggcat gaggaagggg aggaactcgt tgcgatttgg tgtcgatctg
    21361 tcgatgccac ccacgatttt ttatcagcag agtatcgggc cgagctggaa gagctggtgc
    21421 gttcctttct gccggaagca ccgttgtggg tcgcggttaa tgaacgggag cagccggttg
    21481 ggtctatgtt gttaagtggg cagcatatgg atgcgctgtt tatcgatcct gatgtgtgcg
    21541 gctgcggcgt gggtcggatg ctggtggagc atgcgctctc gatggcaccg gaactgacaa
    21601 ccaacgttaa tgagcaaaat gagcaggcgg ttgggttcta taagaaggtg ggttttaagg
    21661 ttacgggacg ctctgaggtg gacgatttgg ggaaaccgta tccgttgctg aatctggcgt
    21721 atgtggggga taagccgccc ctgccgtgcc tgcccaagct ggagaagacg cgccagcgtc
    21781 gcacccggca tttttttcac caacgcaaaa aggccatcct gacggatagc cttt
//
LOCUS       DAJTYF010000062        21722 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTYF010000062 DAJTYF010000000
VERSION     DAJTYF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.062"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..2005
                     /gene="speF"
                     /locus_tag="OO590_002413"
     CDS             <1..2005
                     /gene="speF"
                     /locus_tag="OO590_002413"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR6637748.1"
                     /translation="NEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASHYETQLRP
                     PFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLCNADVAMG
                     DLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHK
                     SNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQRAKEARPF
                     RLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLN
                     ENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFY
                     PLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQS
                     YETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPYKLLLTTPGIDARNGEYEAFGV
                     PATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLA
                     EVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHVSMNPQEA
                     NYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGCAVLRYFSALEEGIN
                     LLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2002..3321
                     /gene="potE"
                     /locus_tag="OO590_002414"
     CDS             2002..3321
                     /gene="potE"
                     /locus_tag="OO590_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6637749.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSITMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="OO590_002415"
     CDS             complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="OO590_002415"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6637750.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="OO590_002416"
     CDS             complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="OO590_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6637751.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5773..6537
                     /gene="ybfF"
                     /locus_tag="OO590_002417"
     CDS             5773..6537
                     /gene="ybfF"
                     /locus_tag="OO590_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6637752.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6677..6970
                     /gene="ybfE"
                     /locus_tag="OO590_002418"
     CDS             6677..6970
                     /gene="ybfE"
                     /locus_tag="OO590_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6637753.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7110..7640
                     /gene="fldA"
                     /locus_tag="OO590_002419"
     CDS             7110..7640
                     /gene="fldA"
                     /locus_tag="OO590_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6637754.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7850..7936
                     /gene="uof"
                     /locus_tag="OO590_002420"
     CDS             7850..7936
                     /gene="uof"
                     /locus_tag="OO590_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6637755.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7929..8375
                     /gene="fur"
                     /locus_tag="OO590_002421"
     CDS             7929..8375
                     /gene="fur"
                     /locus_tag="OO590_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6637756.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8459..8785)
                     /locus_tag="OO590_002422"
     CDS             complement(8459..8785)
                     /locus_tag="OO590_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637757.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="OO590_002423"
     CDS             complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="OO590_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6637758.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLFHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10807..12471)
                     /gene="glnS"
                     /locus_tag="OO590_002424"
     CDS             complement(10807..12471)
                     /gene="glnS"
                     /locus_tag="OO590_002424"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6637759.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12674..14620)
                     /gene="nagE"
                     /locus_tag="OO590_002425"
     CDS             complement(12674..14620)
                     /gene="nagE"
                     /locus_tag="OO590_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6637760.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14953..15753
                     /gene="nagB"
                     /locus_tag="OO590_002426"
     CDS             14953..15753
                     /gene="nagB"
                     /locus_tag="OO590_002426"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6637761.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15813..16961
                     /gene="nagA"
                     /locus_tag="OO590_002427"
     CDS             15813..16961
                     /gene="nagA"
                     /locus_tag="OO590_002427"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6637762.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            16970..18190
                     /gene="nagC"
                     /locus_tag="OO590_002428"
                     /gene_synonym="nagR"
     CDS             16970..18190
                     /gene="nagC"
                     /locus_tag="OO590_002428"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6637763.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18238..18980
                     /gene="nagD"
                     /locus_tag="OO590_002429"
                     /pseudo
     CDS             18238..18980
                     /gene="nagD"
                     /locus_tag="OO590_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19377..21041
                     /gene="asnB"
                     /locus_tag="OO590_002430"
     CDS             19377..21041
                     /gene="asnB"
                     /locus_tag="OO590_002430"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6637764.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21421..21497
                     /locus_tag="OO590_002431"
     tRNA            21421..21497
                     /locus_tag="OO590_002431"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21455..21457,aa:Met,seq:cat)
     gene            21506..21590
                     /locus_tag="OO590_002432"
     tRNA            21506..21590
                     /locus_tag="OO590_002432"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21540..21542,aa:Leu,seq:tag)
     gene            21614..21688
                     /locus_tag="OO590_002433"
     tRNA            21614..21688
                     /locus_tag="OO590_002433"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21646..21648,aa:Gln,seq:ttg)
BASE COUNT         5329 a         5509 c         5486 g         5398 t
ORIGIN      
        1 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
       61 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      121 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      181 ttttgactgc ccgggtcatc agggaggtga gtttttccgt cgtcatccgg cagggaatca
      241 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
      301 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
      361 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
      421 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
      481 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
      541 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
      601 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
      661 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
      721 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
      781 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
      841 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
      901 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
      961 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1021 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1081 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1141 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1201 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1261 agagaatcaa tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc
     1321 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     1381 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
     1441 ggcagaagat atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact
     1501 gcttgagtcc gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga
     1561 gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg
     1621 ccacaacgtg aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag
     1681 catgaatccg caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct
     1741 gccggatgcc gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct
     1801 gtgtgttgtt ccgggtgaaa tctggggttg tgctgttttg cgttacttca gcgctctgga
     1861 agaagggatc aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga
     1921 acatgatggt cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac
     1981 cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag
     2041 ttaaccatac tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag
     2101 cttgccgaag ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg
     2161 gcactggcat gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc
     2221 ggttatgccg agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc
     2281 gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa
     2341 ctgctcggcg cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg
     2401 atttgtaccg tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc
     2461 gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc
     2521 ccgacgcttt acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt
     2581 tcttccatca ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact
     2641 gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt
     2701 gcggcggtga tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag
     2761 ctggcaaatt caacggcacc atttggcctg gccttcgcac agatgttcac accagaagtg
     2821 ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag
     2881 ttcaccattg cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc
     2941 tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag
     3001 agcgggttgc cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt
     3061 aacctggccg tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt
     3121 attcagaagg tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt
     3181 gttggcgcga tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac
     3241 ggttccatcg tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa
     3301 ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt
     3361 tttttattaa atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt
     3421 tctcaatctg cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt
     3481 cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc
     3541 caatagaagc accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca
     3601 gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag
     3661 tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt
     3721 agtgttcctg cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa
     3781 tgatgccgtc cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg
     3841 cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga
     3901 aacctaccgg tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg
     3961 cagaagaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga
     4021 acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga
     4081 cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca
     4141 gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt
     4201 tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga
     4261 ggttgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa
     4321 cgcccagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt
     4381 ccacgaacgg ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga
     4441 gggagatacg cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca
     4501 ccactttggt gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac
     4561 catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt
     4621 ttttattgtg aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt
     4681 cctgcacaat gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag
     4741 gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg
     4801 cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc
     4861 gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg
     4921 cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct
     4981 gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg
     5041 ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg
     5101 atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc
     5161 gtgcctggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct
     5221 gccgcaaagt acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg
     5281 gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc
     5341 tctgcgtatt cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt
     5401 ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga
     5461 gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc
     5521 tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc
     5581 gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa
     5641 taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta
     5701 tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa
     5761 aagaactttg ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat
     5821 tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct
     5881 cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca
     5941 ccaagagagc cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat
     6001 gcactgcaga tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg
     6061 gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg
     6121 gtcgactatc acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa
     6181 tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa
     6241 ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg
     6301 gtattgtggg atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac
     6361 cctgccctgt ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat
     6421 ttactggcac aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc
     6481 catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc
     6541 aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgagtcat
     6601 cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc
     6661 cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc
     6721 gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg
     6781 tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact
     6841 gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag
     6901 ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg
     6961 catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc
     7021 cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt
     7081 tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg
     7141 acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg
     7201 ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc
     7261 tgctgggcat cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc
     7321 cgactctcga agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc
     7381 aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac
     7441 cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa
     7501 aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg
     7561 aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg
     7621 acgaaattct taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag
     7681 ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat
     7741 gtcccagtgt tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac
     7801 aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat
     7861 tatctcaaga gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca
     7921 gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc
     7981 ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag
     8041 atttatacaa acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg
     8101 tactgaacca gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat
     8161 ccgtatttga gctgacacag caacatcacc acgatcacct gatttgcctc gactgcggca
     8221 aggttatcga atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac
     8281 atggcattcg cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt
     8341 gccgcgaaga tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac
     8401 ctgcgggttg gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt
     8461 atttgttatt actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat
     8521 catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct
     8581 ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctggcagg
     8641 cttcaatttt ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca
     8701 gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca
     8761 tggcgatgag aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc
     8821 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
     8881 tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt
     8941 gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc
     9001 tgtaggcaga ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc
     9061 aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa
     9121 gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt
     9181 tgaagtagga acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg
     9241 gggtcatacg ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga
     9301 ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg
     9361 tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact
     9421 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
     9481 attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga
     9541 agtcgtattt cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat
     9601 aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga
     9661 aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag
     9721 tacccggcat aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac
     9781 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac
     9841 cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
     9901 cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat
     9961 caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg
    10021 agtgaaaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac
    10081 gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag
    10141 cctccggcat tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg
    10201 tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc
    10261 accaaaggtg aacagcgaaa aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg
    10321 ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag
    10381 aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt
    10441 gcggttgaaa ggcttgcaga agaataaaaa cgtgaaattc cagcaagatg aaagcggtta
    10501 cattttattt aattatgttt tgtgatttta aatttatttt atggataaat tgcgaagcgg
    10561 aattaattcg cgaagaaaag cagaaaaaac gccgcagagc gcggcgtttg ggattgtttg
    10621 ctatttcttg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca cgcaaattca
    10681 atatattgca gagatcatgt aggcctaata agcgaagcgc atcaggcaat ttagcgtttg
    10741 aaatttattg tctatttatt gccggatgcg acgtaaatgc ctcacccggc ttggtagaac
    10801 ttaaaattac tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac
    10861 cggtttttcg gccgtggaat ggcggctgtc gaggcagaag taaccttcac gctcaaactg
    10921 gaatgcttta cccgctaccg catctttcag cgacggttca gcaaagccct gtttgatcac
    10981 cagcgattcc gggttaatca ccgacaggaa atcatccgca gcacctgggt taggcacgct
    11041 gaacagacga tcatacaaac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg
    11101 aataacacct ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata
    11161 agtacagaag atggtggtga tattaccttc ggcatctttc tccacgcgtt cagccttaat
    11221 gacgtaagca ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt
    11281 agcttcttcg cggaaatcgg cgcgatcgat ccaaatttca ccgctaaacg gcacctgacg
    11341 gctccccatt tccggtttgt tcggatggtt cggcatggtg accatttcgc cttcgccctg
    11401 atagttttcg ataaccagtt tcaccggatc gataactgcc attgcgcgcg gcgcattttc
    11461 gttgagatct tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgctt
    11521 ggtcacgccg atgcgtttgc agaactcacg aatagaagcc gcagtgtaac cacgacgacg
    11581 cagaccggaa atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac
    11641 cagcaggttc agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc
    11701 gtactggcgc ggatgaacag gaatggtgat gttatccagt acccagtcgt acagacgacg
    11761 gttgtcctgg aactcaagcg tacacagaga gtgcgtaata ccttccagcg catcgctgat
    11821 gcaatgggtg aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc
    11881 agcaaattta atgcggtaca gcaccggatc gcgcattacg ataaatggcg acgccatgtc
    11941 gattttcgca cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa
    12001 cagcgccaag ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggctgcgt
    12061 cagggtaccg cggtattcgc ggatctgttc cggtgtcagt tcatcaacgt acgccaggcc
    12121 tttattgatc agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg
    12181 gacgttacca gaccagtgaa aacctaacca ctctacgtcg tttttgatcg aatcgacata
    12241 ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata
    12301 gtcctgggcg atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc
    12361 attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc
    12421 atcgatgatc tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc
    12481 ctcaaagcgt aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact
    12541 gttagttttt taaaattgat aatctaacaa ggattatggg gatgattgat gaaaaaaaaa
    12601 gcggcagaga agatctccac cgcttgtcat tgttggatgc gacgctgccg tgtcgcatca
    12661 ggcataaagc agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc
    12721 cctgagcttt aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa
    12781 tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg
    12841 cgcttacctg cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg
    12901 tgtcgatacc catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt
    12961 ggttggtgtt gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg
    13021 tcggtttcac cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct
    13081 gatccagtgc cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt
    13141 ttggtacagc ctgcggtttt gctacaggcg cggcagttgc cggagttgct tcagctgacg
    13201 cagcggctac cggaccacgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt
    13261 tcgcgccaac aatcacctga atagtctgtt tgttcagttt cactacacca gaagcaccca
    13321 gacgtttaca catcgtatcg ttgacgcggg cagagtcagc cacggtcagg cgcagacggg
    13381 taatacaggc atcaatcgct ttcaggttgt cagtaccgcc aaccgcagca atatagttgg
    13441 ttgccagttg agtcagacct tcttcagtat tgctgttggc ttcttcagta acgatctcgt
    13501 cttctttatc ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca
    13561 ccacgaagta gatagcgaag aagataacgc ccatcaccag cagcatccag acgttctggc
    13621 tggcggccgg caggttatac atcaacgcgt agtcgatagc acccgcagag aaagagaagc
    13681 cggcatggat acccagcagc gttgccacaa acaggctgat accggtcagc agtgcgtgca
    13741 ggaggtacag cagcggcgca aggaacatga acaggaattc cagcggctca gtcacaccgg
    13801 tcaggaacgc agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt
    13861 tcggtgctgc gaagtacatc gccagcgccg cacccggcag accgaacatc atgatcggga
    13921 agaagccgga catgaacatc cccgcggtgc cgtcaccagc gtagaaacgg ttgatgtcgc
    13981 cgtggaaaac cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt
    14041 tcagcacctg atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg
    14101 aacccagcgc gcccgcagaa acgatccact cgccgcctgc atggatagcg tgctgtaccg
    14161 gcggccagac gtaaccaaaa atggccgcca gcaccagaca gaagaagccg gtggcgatcg
    14221 gcacaaagcg tttgccgccg aagaagctca ggaagtccgg cagtttaata tcggaccaac
    14281 ggttataggc tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttaa
    14341 tttctgggtt gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca
    14401 gcgccgccgc acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta
    14461 atgcgaggtt atcaaaaatt gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat
    14521 ctggctgacc gaatcgcagc aacagcgccg ccaccggcag caccgcgata gggagctgta
    14581 acgccctacc gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac
    14641 cctatttggc tcgtttaaag ccgtattttt attttgctgc aaattttact gccgatgttc
    14701 tgtaatcaga ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa
    14761 attaaacgcg tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta
    14821 ctggctaaac cagaaaactt attttatcat tcaaaaaatc aagtcggatt gacgcctgtc
    14881 tgcgcaaatc caggttacgc ttaaagatgt ctaatccgcc aacggcttac attttactta
    14941 ttgaggtgaa taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct
    15001 gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccaa ctgccgatcg tccgtttgta
    15061 ctgggcctgc cgactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat
    15121 aaagcaggcc aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt
    15181 ctgccgaaag agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac
    15241 gttgatattc cagcagaaaa catcaacctt ctcaacggca atgccccgga tatcgatgcc
    15301 gagtgccgcc agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc
    15361 ggtgtaggta acgacggtca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt
    15421 actcgtatca aaaccctgac tcatgacact cgcgtcgcga actctcgttt ctttgataac
    15481 gatgtcaatc aggtgccaaa atatgccctg actgtcggtg ttggtacact gctggatgcc
    15541 gaagaagtga tgattctggt gctgggtagc cagaaagcac tggcgctgca ggccgccgtt
    15601 gaaggttgcg tgaaccatat gtggaccatc agctgtctgc aactgcatcc gaaagcgatc
    15661 atggtgtgcg atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat
    15721 gaattagaag cagaaaatat caaaggtctg taattgttat ccctgccctg gttccttgct
    15781 cagggcaata ttttttaaaa tcgggggtca gaatgtatgc attaacccag ggccggatct
    15841 ttaccggcca cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa
    15901 gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca
    15961 ttctctcccc cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg
    16021 acaccgctga agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat
    16081 caggctgtac taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg
    16141 gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc
    16201 tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta
    16261 agcctgatgc cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga
    16321 ccctggcacc ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg
    16381 tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg
    16441 ggattacctt tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg
    16501 gcctggcggg cgcgatcctc gacgaagctg acatttattg cggtattatc gctgatggcc
    16561 tgcatgttga ttacgccaac attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc
    16621 tggttaccga cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta
    16681 aaacaatata ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat
    16741 ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg
    16801 aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat tgacgttgaa aaacgtctcg
    16861 gcacgctcgc cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat tttaaaatca
    16921 ccaagaccat cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg
    16981 cggacaagct cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta
    17041 tcgcctgatt gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct
    17101 tgcccccgcc agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga
    17161 agttgatcag caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg
    17221 caatttccac gcaatcggcg tacggcttgg tcgtcatgac gccaccatca ctctgtttga
    17281 tctcagcagc aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcagac
    17341 gctggaacat gccctgctga atgccattgc tcagtttatt gatagctacc agcgcaagct
    17401 gcgcgagcta atcgccattt cggtgatcct gccagggctt gttgacccgg acagcggcaa
    17461 aattcattac atgccgcata ttcaggtaga aaactggggg ctggtagaag cactgaaaga
    17521 acgttttaaa gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca
    17581 ctacttcggt gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac
    17641 cggggccggg attatctcta acgggcgcat ttttattggc cgcaacggta acgtcggtga
    17701 aattggccat attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg
    17761 cctggaaact atcgccgcca acgctgccat tgaacaacgg gtgttgaatc tgttaaagca
    17821 gggctaccag agccgtgtgc cactggacga ctgcaccatc aaaactatct gcaaagccgc
    17881 gaacaaaggc gatagcctgg cctcggaagt gattgagtat gtcggtcgtc atctgggtaa
    17941 aaccatcgcc attgctatca acttatttaa tccgcaaaaa attgttattg ctggtgaaat
    18001 caccgaagcc gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaagcgct
    18061 gaaggcgttt cgtactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat
    18121 cggcgctttt gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct
    18181 ggaaaattaa tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg
    18241 accattaaaa atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta
    18301 ccgggtgcag cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg
    18361 accaactatc cttcgcagac tgggcaagat ctggcgaacc gctttgccac tgccggtgtc
    18421 gatgtgccgg atagcgtgtt ttatacctct gcgatggcga ccgccgattt cctgcgtcgc
    18481 caggaaggca agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa
    18541 gccggtttca ctattaccga tgtgaaccct gatttcgtga ttgttggcga aacacgttcc
    18601 tacaactggg acatgatgca taaagcagcc tatttcgtcg ctaacggtgc acgctttatc
    18661 gccaccaacc cggacacggt ttttatcccg cttgtggcgc gttgtgtgca gggattgaga
    18721 aaatctccgg gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc atccgcgcgg
    18781 cattaaacaa aatgcaggcg cattcggaag aaacagtgat tgtcggcgat aacctgcgta
    18841 ccgatattct ggcgggcttc caggccggtc tggagacgat tctggtgctt tctggtgttt
    18901 catcgctcga cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcagtcg
    18961 ctgaaatcga cgttatctga ataccaccca cgtctgcgga cgtggttggc tctgatgaca
    19021 aacgccaaac tgcctgatgc gctacgctta tcaggcctac gcagctcctg caatatattg
    19081 aattttcatg cttttgtagg ccgtataagg cgttcacgcc gcatccggca tgaacaaatc
    19141 gcactttatc agcaatttgg cccccgtaaa atataaccat tcaataaaac ccgctaaaaa
    19201 ttatcgattc atcaataaat acatcctcac accagaccct ttttcaccaa tcgacaatca
    19261 ccattacgtt ttttattttt gcactgccaa atagcttgcg cacagtgctg ctttgcggca
    19321 tttttttaaa caagcaaaca caacaagcaa caaataccag gttaacggag aaggttatgt
    19381 gttcaatttt tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac
    19441 tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg
    19501 ataacgccat tctcgctcac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac
    19561 ctctctacaa ccaacaaaaa acccacgtac tggcggtaaa cggtgaaatc tacaaccacc
    19621 aggcattgcg cgctgaatat ggcgatcgtt atcagttcca gacagggtct gactgtgaag
    19681 tgatcctcgc gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt
    19741 ttgcctttgc cctgtacgac agcgaaaaag atgcctacct gattggtcgc gaccatctgg
    19801 ggatcatccc actgtatatg gggtatgacg aacacggtca gctgtatgtg gcctcagaaa
    19861 tgaaatcgct ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg agctatttgt
    19921 ggagtcagga cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tttgatgcag
    19981 tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa
    20041 gccatctgat gtctgatgtg ccttacggtg tgctactttc tggtggtctg gattcctcaa
    20101 ttatttccgc tatcaccaag aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg
    20161 aagcctggtg gccgcagtta cactcctttg ctgtaggtct gccgggttca ccggatctta
    20221 aagcagccca ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg
    20281 tacaggaagg tctggatgcc atccgcgacg tgatttacca catcgaaact tatgatgtga
    20341 ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcgatgggca
    20401 ttaaaatggt gctgtccggt gaaggttctg atgaagtgtt tggcggttat ctttacttcc
    20461 ataaagcgcc caacgctaaa gaactgcatg aagagacggt gcgtaaactg ctggccctgc
    20521 atatgtatga ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg
    20581 ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg caggataaaa
    20641 tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag tcatacctgc
    20701 ccgcaagcgt ggcctggcgg cagaaagagc agttctccga tggcgtcggt tacagttgga
    20761 tcgacaccct gaaagaagtg gcggcgcagc aggtttctga tcagcaactg gaaactgccc
    20821 gcttccgctt cccgtacaac acgccgacct caaaagaagc gtatctgtac cgcgagatct
    20881 ttgaagaact gttcccactt ccgagtgccg ctgagtgcgt gccgggcggt ccttccgtcg
    20941 cgtgttcttc agctaaagcg atcgaatggg atgaagcgtt caaaaaaatg gacgatccgt
    21001 ctggtcgtgc ggttggcgtt caccagtcgg cgtataagta agaaatgcgg tactgccccg
    21061 gagaaatccg gggctgtttt agttattgcg ctgcttgttg attctgatat gcagtaagga
    21121 aatgttccgg gtttgccaca tcactgtttg acaactcatc actgtaggcc agtatttcat
    21181 agattaattt cccaccacta ttgttgaatt ccgaatttca tcctgtacga caaaagaatg
    21241 gctgtagggt ggggaaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg
    21301 ttcgcaacct aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct
    21361 gcaaggctct atacgcataa tgcgccccgc aacgcagata aggtatcgcg aaaaaaagat
    21421 ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca caggttcgaa
    21481 tcccgtcgta gccaccatct tttttgcggg agtggcgaaa ttggtagacg caccagattt
    21541 aggttctggc gccgcaaggt gtgcgagttc aagtctcgcc tcccgcacca ttcaccagaa
    21601 agcgttgtac ggatggggta tcgccaagcg gtaaggcacc ggtttttgat accggcattc
    21661 cctggttcga atccaggtac cccagccatc ttcttcgagt aagcggttca ccgcccggtt
    21721 at
//
LOCUS       DAJTYF010000063        21496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTYF010000063 DAJTYF010000000
VERSION     DAJTYF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.063"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(9..1007)
                     /gene="fbp"
                     /locus_tag="OO590_002434"
     CDS             complement(9..1007)
                     /gene="fbp"
                     /locus_tag="OO590_002434"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6637765.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1183..2556
                     /gene="mpl"
                     /locus_tag="OO590_002435"
     CDS             1183..2556
                     /gene="mpl"
                     /locus_tag="OO590_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6637766.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLHD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2639..3190)
                     /locus_tag="OO590_002436"
     CDS             complement(2639..3190)
                     /locus_tag="OO590_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6637767.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3284..4636
                     /gene="pmbA"
                     /locus_tag="OO590_002437"
     CDS             3284..4636
                     /gene="pmbA"
                     /locus_tag="OO590_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6637768.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4819..5205
                     /gene="cybC"
                     /locus_tag="OO590_002438"
     CDS             4819..5205
                     /gene="cybC"
                     /locus_tag="OO590_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR6637769.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5250..5714)
                     /gene="nrdG"
                     /locus_tag="OO590_002439"
     CDS             complement(5250..5714)
                     /gene="nrdG"
                     /locus_tag="OO590_002439"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6637770.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5872..8010)
                     /gene="nrdD"
                     /locus_tag="OO590_002440"
     CDS             complement(5872..8010)
                     /gene="nrdD"
                     /locus_tag="OO590_002440"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6637771.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8404..10059)
                     /gene="treC"
                     /locus_tag="OO590_002441"
     CDS             complement(8404..10059)
                     /gene="treC"
                     /locus_tag="OO590_002441"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6637772.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10109..11530)
                     /gene="treB"
                     /locus_tag="OO590_002442"
                     /pseudo
     CDS             complement(10109..11530)
                     /gene="treB"
                     /locus_tag="OO590_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11649..12596)
                     /gene="treR"
                     /locus_tag="OO590_002443"
     CDS             complement(11649..12596)
                     /gene="treR"
                     /locus_tag="OO590_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6637773.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12781..12834
                     /gene="mgtL"
                     /locus_tag="OO590_002444"
     CDS             12781..12834
                     /gene="mgtL"
                     /locus_tag="OO590_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6637774.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12975..15671
                     /gene="mgtA"
                     /locus_tag="OO590_002445"
     CDS             12975..15671
                     /gene="mgtA"
                     /locus_tag="OO590_002445"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6637775.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15877..16263)
                     /gene="ridA"
                     /locus_tag="OO590_002446"
     CDS             complement(15877..16263)
                     /gene="ridA"
                     /locus_tag="OO590_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6637776.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16336..16797)
                     /gene="pyrI"
                     /locus_tag="OO590_002447"
     CDS             complement(16336..16797)
                     /gene="pyrI"
                     /locus_tag="OO590_002447"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6637777.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16810..17745)
                     /gene="pyrB"
                     /locus_tag="OO590_002448"
     CDS             complement(16810..17745)
                     /gene="pyrB"
                     /locus_tag="OO590_002448"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6637778.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSGNTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17749..17883)
                     /gene="pyrL"
                     /locus_tag="OO590_002449"
     CDS             complement(17749..17883)
                     /gene="pyrL"
                     /locus_tag="OO590_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6637779.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18164..18559)
                     /locus_tag="OO590_002450"
     CDS             complement(18164..18559)
                     /locus_tag="OO590_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6637780.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18691..19404)
                     /gene="bdcA"
                     /locus_tag="OO590_002451"
     CDS             complement(18691..19404)
                     /gene="bdcA"
                     /locus_tag="OO590_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6637781.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19475..20068
                     /locus_tag="OO590_002452"
     CDS             19475..20068
                     /locus_tag="OO590_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6637782.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20213..20665
                     /gene="tabA"
                     /locus_tag="OO590_002453"
     CDS             20213..20665
                     /gene="tabA"
                     /locus_tag="OO590_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6637783.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20788..>21496
                     /locus_tag="OO590_002454"
     CDS             20788..>21496
                     /locus_tag="OO590_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6637784.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLE"
BASE COUNT         5195 a         5328 c         5654 g         5319 t
ORIGIN      
        1 gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc aaccatatgg
       61 tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat atccagaata
      121 cgctctttgc catcgctcgc tttaccaccc gcttgttccg ccaggaatgc catcgggttg
      181 cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg gtagagataa
      241 ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat ataacgcgag
      301 gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta cttcttcacc
      361 ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc tttctccggg
      421 aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta agtaaaggcg
      481 tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac cacgtaacct
      541 gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg cgtgccaaca
      601 ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac atcgatgttg
      661 gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca gccttcaaag
      721 acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg tgctttcagt
      781 gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc gccctgcacg
      841 ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat atcgcgatgg
      901 ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc ggtagcatga
      961 gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa actttccctg
     1021 caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa tgttgcgctc
     1081 aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac ttgcccgaca
     1141 tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat tcatatttta
     1201 ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt aggccatgaa
     1261 gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga gaagcaaggc
     1321 attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga tctggtgatt
     1381 attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga aaaaaacatc
     1441 ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcacgaccg ctgggtgcta
     1501 gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg gattctggaa
     1561 cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt tgaggtttcg
     1621 gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga ctgcgccttc
     1681 ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct caacaacctt
     1741 gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca gttccaccat
     1801 ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga catcaacctg
     1861 aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga gcagggtcac
     1921 tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct ggatggcgaa
     1981 aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa tggcctgatg
     2041 gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa cgcgctgggt
     2101 tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg cgtcacggta
     2161 tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc gctgcgtggc
     2221 aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa taccatgaaa
     2281 atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga agtcttcctg
     2341 ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg cgttcagcct
     2401 gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac cgctcagcct
     2461 ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca gaaactgctg
     2521 gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct gatatagcat
     2581 tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac aacgaaaatt
     2641 acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg gatttcggcg
     2701 gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt tgctgacgat
     2761 cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga tcgatcagac
     2821 gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga cggtttttca
     2881 gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc ttaccgatga
     2941 gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca atagccgggc
     3001 gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca acaatttccg
     3061 cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg acccagataa
     3121 tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag tcttcgggct
     3181 gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa gatgcgcact
     3241 gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac ttgcaatgaa
     3301 agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt ccactgcgct
     3361 ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga ccaccggcat
     3421 tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg atggcgcgct
     3481 ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg atttaagccc
     3541 gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata cctcgccaga
     3601 tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga atctcgactt
     3661 gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc gcgcagaaca
     3721 ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct ttaacagcca
     3781 ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact gctcaacgcg
     3841 tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc gtgattacgc
     3901 ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg gggccgactg
     3961 tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga aagcgccggt
     4021 catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg cgatagcggg
     4081 tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac aaattctgcc
     4141 ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt cgacgccatt
     4201 cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca tcctgactca
     4261 gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg gacatgcggg
     4321 cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa tgctcaaaga
     4381 gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg ccattaccgg
     4441 tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc agtatcccgt
     4501 tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg tcaccgtcgg
     4561 taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc cggagatgaa
     4621 aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa aaattacaca
     4681 aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg tactcgcagt
     4741 acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat gaagtgtaaa
     4801 taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt ctcctcgttg
     4861 gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac cctcaacgac
     4921 aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc gttaacgaag
     4981 atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga agataaatca
     5041 ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt cggtcagatt
     5101 gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc tgctgcagag
     5161 caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct catttccctg
     5221 ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac ctgattgctg
     5281 ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc gtcgaccagc
     5341 acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag tttatagcct
     5401 gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac cagtttcaga
     5461 atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat cccctggcgt
     5521 ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt ggtaaatggc
     5581 tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc atgaacacac
     5641 ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac aggatagtac
     5701 tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc atccggtaat
     5761 caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc aaatgtaggc
     5821 cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag attaacctat
     5881 ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac cagcgttaaa
     5941 cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg acacacggga
     6001 ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact cgaactcacc
     6061 ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat acggtacatg
     6121 ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa tgtttggata
     6181 ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt caaagtcgat
     6241 cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg tgtagtaacc
     6301 tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc agaagcggtc
     6361 gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt cttctttcca
     6421 ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc gaagcttctc
     6481 gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga tgccgatgta
     6541 acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat cgtcagcatt
     6601 cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg ctttcacgcc
     6661 ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac gttcatccag
     6721 cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg gcaggttcag
     6781 gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt cattttccca
     6841 cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga ctttcactac
     6901 ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca gcgccagctg
     6961 tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa tcgcaaacac
     7021 cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac ggttacgcag
     7081 gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac caaaggttac
     7141 aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca gcgactggaa
     7201 ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt ccgggatgtt
     7261 ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg gcgccagcac
     7321 ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct gagcaataat
     7381 ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct cggcgttccc
     7441 cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt tgaacatcgg
     7501 gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat gtgcctgcac
     7561 cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc cggccagcag
     7621 gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga gtaacgaggc
     7681 gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt tttcacgttc
     7741 aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt atggacccga
     7801 catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt tgcgcccctg
     7861 catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat cgacttccgc
     7921 tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg gcactttgca
     7981 gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag aacaggttat
     8041 ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg tgctttccgc
     8101 aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt agtagcaatt
     8161 atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata tatagtgctt
     8221 tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca gagcgtacgg
     8281 atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg aaaaatcaac
     8341 aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg gcttagggaa
     8401 gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg cacagggttg
     8461 tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca tttgccctgg
     8521 ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt gccccttcca
     8581 ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt aattgcccca
     8641 tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt aaaacacaga
     8701 ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc ctaggccaat
     8761 ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca tgggcgtgcg
     8821 gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat cacgaccatc
     8881 gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag taatgcgcgt
     8941 gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg ttccctgcat
     9001 gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac cttcatcacc
     9061 aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat tccatgtcac
     9121 gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa agtcaggttt
     9181 agccaacgtc catttttcac caccgggata atcgaccttc aggtgatgaa aattaaaggt
     9241 catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa ggctggtgga
     9301 ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt tcatctcgtg
     9361 caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc catccaggtc
     9421 ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca acccgtcgac
     9481 cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg ctggattctc
     9541 ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt cgctttccgt
     9601 atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg gcgtttctgg
     9661 ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca gcgcctcgcg
     9721 aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga tacgaatccc
     9781 gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt aggtgggatc
     9841 aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga cataaaaggg
     9901 ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt ggataacgcc
     9961 acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat aaatctggta
    10021 gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc tgcaaatgcg
    10081 agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc aggcggtatt
    10141 tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata gccattgcca
    10201 gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg ccccctacgc
    10261 cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga ccagaaccaa
    10321 tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt gcaggctcag
    10381 tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca ttgtgcttgc
    10441 ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac agcgcaatca
    10501 gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata gcaagggtgg
    10561 tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat gccgcaccaa
    10621 tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca tcgccaatca
    10681 tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc agcgaacaga
    10741 cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga gtttcaataa
    10801 cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag cccactttgg
    10861 cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc agataagcgt
    10921 tcatcagctg ggaggaaacc agtgtcacgc caagcacgat accaaggatc ggcgtgccgc
    10981 ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa atcgcttcac
    11041 cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc gccagcgtct
    11101 gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc aaaccgccgc
    11161 taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc aactgctcat
    11221 gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc tgcgcctgtc
    11281 cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc accacctgaa
    11341 attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt tctttcggtc
    11401 tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg ctcaccgtcg
    11461 caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt tggtttattt
    11521 tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa tatattgcgc
    11581 ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat ttatcgttca
    11641 ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct gcgcccggtt
    11701 acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc aggatctacg
    11761 gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct cgccagttgc
    11821 aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag cgtgtcggtt
    11881 gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc atagccttgc
    11941 ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt gcagaacgcc
    12001 aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac gccgagataa
    12061 ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat tgccccttcg
    12121 tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac cagcgatgac
    12181 tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac tacgccgtcg
    12241 atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga aaactgattt
    12301 tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt ttgaacggcg
    12361 agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt atcgctttgc
    12421 ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat cactgcttca
    12481 acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg ggaaaccgta
    12541 gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt ttgcatcctg
    12601 ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca ttcagcaatg
    12661 ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc cataaatgct
    12721 acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac cggaggttat
    12781 atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg gtaagcccgt
    12841 cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct gctcagcttt
    12901 attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt tcagacagtg
    12961 cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat ttaccttccc
    13021 gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg gcggtcccgc
    13081 cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa ttgtggaaaa
    13141 cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc cgcgaacaac
    13201 atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat ttatgggtct
    13261 gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct tacgccacgg
    13321 aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg ttgctgaact
    13381 ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg gtcagtaata
    13441 ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg gagatcccga
    13501 tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg atcccggcag
    13561 atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta accggtgagt
    13621 ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat ccgctggagt
    13681 gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa gcaatggtga
    13741 ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt gagcaggaaa
    13801 gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg attcgcttta
    13861 tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc gactggtggg
    13921 aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg ttgccgatga
    13981 ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa gtgatcgtca
    14041 aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact gataaaaccg
    14101 gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc ggtaaaacca
    14161 gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga cttaaaaatc
    14221 tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg ctggccagcc
    14281 gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg tcggtggtag
    14341 tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag gaaatcctca
    14401 atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac accatgctgc
    14461 gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg gttgcggtgg
    14521 cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa tccgacctga
    14581 tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct ccggcactga
    14641 aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt gagttagttg
    14701 cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt ggtagtgata
    14761 ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg ctgtttgccc
    14821 gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa gggcatgtgg
    14881 ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg gatatcggca
    14941 tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc ctgctggaaa
    15001 aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc gccaacatgc
    15061 tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc gtgctggtgg
    15121 cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt cagaacctgc
    15181 tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag caaattcaaa
    15241 agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc ggaccgatca
    15301 gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat gccaacacgc
    15361 cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg tcgcaaacgc
    15421 tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt gcatcgtggc
    15481 cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg ttttcaccgc
    15541 tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg ctggttgcga
    15601 ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc cgtcgttacg
    15661 gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag acaaagtgag
    15721 ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc aataaattgc
    15781 aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt gtcatcagac
    15841 ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag cgattgcttc
    15901 gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac gtgccgggaa
    15961 agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg cgaagtcgtt
    16021 cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc cagcggcttc
    16081 gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt ctgccggtac
    16141 ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat tgcccagatc
    16201 aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga tagttttgct
    16261 catgatttct cctttattac agcggtaaaa agtctggctc attataggga gccagacttt
    16321 tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt tttcacagta
    16381 tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg atgaaaccgg
    16441 ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga tgcgctccgg
    16501 cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa ccgtggcttg
    16561 cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat tttcgatttt
    16621 gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg tgatgcgctg
    16681 atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg cggggatatg
    16741 gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt gtgtcatctc
    16801 tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg cctggcgagc
    16861 gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat ccacatccgt
    16921 cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt tggcgttgtg
    16981 gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg acgggtccag
    17041 acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca cttcttcaat
    17101 agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa tgtattgcgg
    17161 cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact tcgctagcgc
    17221 ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg cgacgtggag
    17281 attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg tttgcgtcgg
    17341 atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa attcggtggc
    17401 caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt aagtgctgat
    17461 aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat tgccgctgtc
    17521 ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag agaggcgggt
    17581 acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca gctctggttg
    17641 cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat cgcgactaag
    17701 gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt atctcctgac
    17761 gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca acgggaagaa
    17821 aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa cacactgaac
    17881 catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg gtgcaagcat
    17941 ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa aatatatgca
    18001 tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat tgcagacatt
    18061 tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc cattcatccg
    18121 acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc tcagggatac
    18181 gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc accgccgtcc
    18241 agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg tcttcaaatt
    18301 gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa gtgcatcccg
    18361 cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc tgaaaatcgg
    18421 gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc aaatcgccgg
    18481 aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca gcagggaaaa
    18541 cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca gaagatttat
    18601 atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag acatcatgga
    18661 taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat caatggtatg
    18721 catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca taccagcgac
    18781 ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat cgcgcattgg
    18841 cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt tgatggtgat
    18901 ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg cagatttact
    18961 ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg agccgatgat
    19021 taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat gataaggagc
    19081 atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca gggcatcgcc
    19141 aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct tacgaacgac
    19201 atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc cagtctcttg
    19261 tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat tggccccatc
    19321 ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga ggatgagaac
    19381 tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac atgcattaca
    19441 aaactgtttt aactttctgt caacaggttt tgtaatggtt actaaaaaac aatctcgcgt
    19501 tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa aagtgctttt
    19561 tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc ttggtattaa
    19621 ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc gtgtactcaa
    19681 tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg atcgtccggt
    19741 aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc aaaacggcgg
    19801 ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag cgcgtgatat
    19861 tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg ccaggcggca
    19921 tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag ggctttctgc
    19981 gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc tggcggggga
    20041 agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc gggaacgcgc
    20101 ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga attgatcatc
    20161 ttttcgtata acgatagaaa tgaaacattg ttttaattaa ggagtggaaa agatgatcat
    20221 cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg cgattgagca
    20281 tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg aaggcaatcg
    20341 tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc gtgcggagta
    20401 ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca tgaccttcag
    20461 cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca tcgcattttt
    20521 gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg ttgtgtttta
    20581 tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg ttcgcaaagc
    20641 agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca ggtttacggc
    20701 gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt aatgcttaga
    20761 cgttcttatt aactcaagga gttcgtcatg agcaaaatat cagatttgaa ttattctcaa
    20821 cacattacat tagccgacaa ttttaaacaa aaaaatgaag ccttagatac ctggtatgtg
    20881 ggaatgaatg attttgcccg aattgccggg gggcagaata gcagaagcaa tattctttct
    20941 cctagagcat ttttggagtt tttggcaaag atatttaccc taggttatgt ggattttagc
    21001 aaacgctcca acgaagcggg tagaaatatg atggctcata ttgagttctc atcttattct
    21061 aaagatactg atggcaatga aaaaatgaag ttttacatga ataatcctga aggggagcga
    21121 gcggatttat ctaaggtgaa gatagaaatt acacttgcct cagcttctac taaagggatt
    21181 cgtgaaggac atacagtcat tatatttaaa caatctgatg gttcgactaa tcgttatgaa
    21241 gggaagtctt ttgaaagaaa agatgatagt tcattacacc tgattactaa caaggttctg
    21301 gcgtgttacc aacgcgaagc taacaagaaa atagcgcggc tattaaataa tcatcagaag
    21361 ttaaataata tacaggagtt aaatgattcg caggagttaa ataattcaca gaagttaaat
    21421 aattcgcagg agttaaataa ctcgcaggtg agttgtaaag gttcagttga ttctacgatt
    21481 acggatttat tagaaa
//
LOCUS       DAJTYF010000064        21239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTYF010000064 DAJTYF010000000
VERSION     DAJTYF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.064"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(32..1012)
                     /locus_tag="OO590_002455"
     CDS             complement(32..1012)
                     /locus_tag="OO590_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6637785.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1020..1730)
                     /locus_tag="OO590_002456"
     CDS             complement(1020..1730)
                     /locus_tag="OO590_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR6637786.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1946..3769)
                     /gene="typA"
                     /locus_tag="OO590_002457"
     CDS             complement(1946..3769)
                     /gene="typA"
                     /locus_tag="OO590_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6637787.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4142..5551
                     /gene="glnA"
                     /locus_tag="OO590_002458"
     CDS             4142..5551
                     /gene="glnA"
                     /locus_tag="OO590_002458"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6637788.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5724..6773
                     /gene="glnL"
                     /locus_tag="OO590_002459"
     CDS             5724..6773
                     /gene="glnL"
                     /locus_tag="OO590_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6637789.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6785..8194
                     /gene="glnG"
                     /locus_tag="OO590_002460"
     CDS             6785..8194
                     /gene="glnG"
                     /locus_tag="OO590_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6637790.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8306..8416
                     /locus_tag="OO590_002461"
     CDS             8306..8416
                     /locus_tag="OO590_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6637791.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8645..10018)
                     /gene="hemN"
                     /locus_tag="OO590_002462"
     CDS             complement(8645..10018)
                     /gene="hemN"
                     /locus_tag="OO590_002462"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6637792.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10207..10716)
                     /gene="yihI"
                     /locus_tag="OO590_002463"
     CDS             complement(10207..10716)
                     /gene="yihI"
                     /locus_tag="OO590_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6637793.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11298..11930
                     /locus_tag="OO590_002464"
     CDS             11298..11930
                     /locus_tag="OO590_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6637794.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12312..15098)
                     /gene="polA"
                     /locus_tag="OO590_002465"
     CDS             complement(12312..15098)
                     /gene="polA"
                     /locus_tag="OO590_002465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6637795.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYTEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15462..16394
                     /locus_tag="OO590_002466"
     CDS             15462..16394
                     /locus_tag="OO590_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6637796.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16435..17865)
                     /locus_tag="OO590_002467"
                     /pseudo
     CDS             complement(16435..17865)
                     /locus_tag="OO590_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
     gene            complement(18020..18646)
                     /gene="dsbA"
                     /locus_tag="OO590_002468"
     CDS             complement(18020..18646)
                     /gene="dsbA"
                     /locus_tag="OO590_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6637797.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18663..19649)
                     /gene="srkA"
                     /locus_tag="OO590_002469"
     CDS             complement(18663..19649)
                     /gene="srkA"
                     /locus_tag="OO590_002469"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6637798.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRQTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19726..19995)
                     /locus_tag="OO590_002470"
     CDS             complement(19726..19995)
                     /locus_tag="OO590_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6637799.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20065..20649
                     /gene="mobA"
                     /locus_tag="OO590_002471"
     CDS             20065..20649
                     /gene="mobA"
                     /locus_tag="OO590_002471"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6637800.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20631..21158
                     /gene="mobB"
                     /locus_tag="OO590_002472"
     CDS             20631..21158
                     /gene="mobB"
                     /locus_tag="OO590_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6637801.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5178 a         5346 c         5120 g         5595 t
ORIGIN      
        1 gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt
       61 gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt
      121 ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc
      181 gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat
      241 caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa
      301 ggtgcgttaa taacgctttg aatggcatat caccctgacc tgaaatacag gctttatgac
      361 ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg
      421 gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttagcaaaat
      481 caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc agctcatgac
      541 tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc gctgtcctga tacgtttccc
      601 gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac
      661 gtggataaaa acgtacggac gttgccccag ttttcaacgc gatggcgacg gcttcgtcaa
      721 tagatgcctt atctgaagcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt
      781 tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat
      841 ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag
      901 caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag
      961 gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc
     1021 tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca
     1081 atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa
     1141 ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt
     1201 actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt
     1261 tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta
     1321 tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg cgtcactgca
     1381 atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt aaacccccaa
     1441 gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag ctccggactg
     1501 taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat
     1561 tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct
     1621 cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac
     1681 cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc
     1741 ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg
     1801 tgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa
     1861 gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaacctgcc atcgctggca
     1921 ggttttttat gactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg gcgcggcgac
     1981 gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc acttctacca
     2041 gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata ggcggaacca
     2101 gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta ccggtcaggc
     2161 agtttacagt caggtcgtta gagcggctat gaataccgat aatctgacct tcgtaaactt
     2221 ctgcaccgtg accgaggaac agcttaccgc gatcctgcag accgaacagc gcgaacgcga
     2281 cagctttacc ctgaccgtta gagatcagta caccgttctg acgctgaccc acttcgcccg
     2341 gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg gaagtcatgg
     2401 tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag tcgagacgta
     2461 cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg cccagcgcct
     2521 gcatcacaga accctgatgc tgttcttcaa cgtccagcgt cacgttttca tacggctctt
     2581 gtttacgacc gtcgatttca cggaagatta ctttcggacg ggataccgcc agttcgaaac
     2641 cttcacgacg catgttttcg ataagaacag agaggtgcag ttcaccacga ccagaaacgc
     2701 ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt accagttctt
     2761 tgttcagacg atcgaggatc tgacgagacg ttacgaactt accttcttta ccgcagaacg
     2821 gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag agtgccggca
     2881 gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg cccaggcccg
     2941 tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc agaccgaggt
     3001 ggcccagcac tttaccgact ttcgcgttac gggttttgcc ttcgctatcg atgatagtaa
     3061 cctgctggtt cggcttcact ttaccacgct tgatgcggcc aatgccgata acgccaacat
     3121 agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg tcaacgtccg
     3181 gcgcaggaac gtggtcaaca atcgcctgat acagcggggt catgtcttcc gccatatctt
     3241 cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg aagtccagct
     3301 gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca acccaatcag
     3361 gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg ccgtaagcaa
     3421 acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg tcaaccacca
     3481 gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag tcggcgtgcc
     3541 ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg tttttcgcga
     3601 ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc tcttgggttt
     3661 cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccaag gtggttttac
     3721 catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca actttgcctc
     3781 aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa caggatcaca
     3841 aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac ggagcataaa
     3901 aagggttatc caaaggtcat tgcaccaata tggtgcttaa tgtttccatt gaagcactat
     3961 attggtgcaa cattcacatc atggtgcagc ccttttgcac gatgttaagc atgataacgc
     4021 cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta cggcgacacg
     4081 gccaaaataa ttgcagattt cgttaccacg acgacaatga ccaatccagg agagttaaag
     4141 tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt ttgttgattt
     4201 gcgcttcacc gataccaaag gtaaagaaca acacgtcact atccctgctc atcaggtgaa
     4261 tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg gctggaaagg
     4321 cattaacgaa tccgacatgg tgctgatgcc agacgcatcc accgcagtga ttgacccgtt
     4381 cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca ccctgcaagg
     4441 ctatgaccgt gacccgcgct ctattgcgaa gcgcgccgaa gattacctgc gttccactgg
     4501 cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg atgacatccg
     4561 tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag gcgcatggaa
     4621 ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga aaggcggtta
     4681 cttcccggtt ccgccggtag actctgctca ggatattcgt tctgaaatgt gtctggtgat
     4741 ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg ctggtcagaa
     4801 cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc agatctacaa
     4861 atatgtcgtg cataacgtag cgcaccgctt cggtaaaacc gcgaccttta tgccaaaacc
     4921 gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta aaaacggcgt
     4981 taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt actacattgg
     5041 cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca ccaactctta
     5101 taagcgtctg gtcccgggct acgaagcacc ggtaatgctg gcttactctg cgcgtaaccg
     5161 ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta tcgaagtacg
     5221 tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga tggccggtct
     5281 tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc tgtatgacct
     5341 gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag aagcactgaa
     5401 cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcacgg atgaagcgat
     5461 cgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc gtgcgtatga ctccgcatcc
     5521 ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc cggcgcacaa
     5581 cgccgggatt tagttgccgt ggaaactttc agcccatctc tgcatgggct tttttctccg
     5641 tcaattctct gatgcttcgc gctttttatc cgtaaaaagc tataatgcac taaaatggtg
     5701 caacctgttc aggagactgc tttatggcaa caggcacgca gcccgatgct gggcagatcc
     5761 tcaactcgct gattaacagt attttgttaa ttgatgacaa tctggcgatc cattacgcca
     5821 accccgccgc gcaacaactg ctcgcccaaa gctcccgcaa attgtttggt acgccgttac
     5881 cggaactgtt gagctacttc tcattaaata tcgagctgat gcaagaaagt ctggaggcgg
     5941 ggcaaggctt taccgataac gaagtgacgc tggtcatcga tggtcgctcg catatccttt
     6001 ctgtgacggc ccagcgtatg ccggacggca tgatcctgct ggagatggca ccgatggata
     6061 accagcgccg cttaagccag gaacaactac aacatgctca acaagtagct gcccgtgatt
     6121 tagtgcgcgg cctggcacat gagattaaaa atccgcttgg cggtttacgt ggcgcggcgc
     6181 agttactcag caaagcgtta cctgacccat cactactcga atataccaaa gtgattatcg
     6241 aacaggcgga ccggctgcga aatttggtcg accgtctgtt ggggccgcag ctgcccggta
     6301 cgcgcgttac cgaaagtatt cacaaagtgg ctgaacgcgt ggtaacgctg gtgtcgatgg
     6361 aactgccgga caacgtgcgg ttgattcgtg attacgatcc cagcctgccg gaactggcgc
     6421 acgacccgga tcaaattgaa caggtcttgc tgaatattgt gcgcaatgcg ctacaggcgc
     6481 tggggccgga aggcggtgaa atcattctgc gtacccgcac cgcgtttcaa ctgaccttac
     6541 acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga agataacggg ccgggcattc
     6601 cgcctcattt gcaggatacg ctgttttacc cgatggtcag cggccgcgaa ggtggcaccg
     6661 ggcttggctt atccatcgcc cgtaatttga ttgatcagca ttcaggcaaa attgaattta
     6721 ccagttggcc aggtcatacc gagttctcgg tttacctgcc tatcaggaaa taaaggtgac
     6781 gtttatgcaa cgagggatag tctgggtagt cgatgacgat agttccatcc gttgggtgct
     6841 tgaacgtgcg ctcgctggag cgggtttaac ctgtacgaca tttgagaacg gcgcggaagt
     6901 actggaggcg ctggcgagca aaacgccgga tgtgctgctt tcagatatcc gtatgccggg
     6961 aatggacggg ctggcgctgc tcaagcagat taaacagcgc catccaatgc ttccggtcat
     7021 cattatgacc gcacattccg atctggatgc tgccgtcagc gcctatcaac aaggggcgtt
     7081 tgattatctg cccaaaccgt ttgatatcga cgaagcagtg gcgctggttg agcgcgctat
     7141 cagtcattac caggaacagc agcagccgcg taatattcag cttaacggcc caacgaccga
     7201 tatcatcggc gaagcgcaag ccatgcagga cgtgttccgt attatcggtc ggctttcgcg
     7261 ttcttctatt agcgtgctga ttaacggcga atccggcacc ggtaaagaac tggtcgctca
     7321 tgccctgcat cgccacagtc cgcgcaccaa agcgccgttt atcgcgctga atatggcagc
     7381 tatcccaaaa gatttgatcg aatcagaact gtttggccac gagaaaggcg cgtttactgg
     7441 cgcgaatacc attcgtcagg ggcgttttga acaggccgat ggcggcacgt tatttctcga
     7501 cgaaattgga gatatgccgc tggatgtgca gacgcgtttg ctgcgcgtgc tggcagacgg
     7561 tcagttttat cgcgttggcg gctatgcgcc ggtgaaagtg gatgtgcgga ttatcgctgc
     7621 cactcaccag aatcttgaac agcgggtgca ggaaggtaag ttccgtgagg atctgttcca
     7681 ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc gaacgtcggg aagatattcc
     7741 ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa ctgggcgtag aagcgaagtt
     7801 gctgcatccg gaaaccgaag ctgctctgac gcgtctggcg tggccaggca acgtgcgcca
     7861 gctggaaaac acctgccgct ggctaacggt gatggccgcc gggcaggaag tgttgattca
     7921 ggatttgccc ggcgaactgt ttgaatcaac ggttgcggag agtacttcgc aaatgcaacc
     7981 ggacagttgg gcgacacttt tagcgcagtg ggcagacaga gcgctgcgtt ccggtcatca
     8041 aaatctgctt tccgaagcgc agccagagct ggagcggacg ttactgacga ccgcgttgcg
     8101 acatacgcag gggcataaac aggaagcggc gcggctactc ggctggggcc gcaacactct
     8161 gacgcgtaag ttaaaagagc tggggatgga gtgattcacg gcttgtgtgt aaagattgat
     8221 tattgagcgc aaattgctgg tattttgcgc tttactgttc cgataagttc agtatgatct
     8281 tgcccggaaa acggggagag tcattatgct ggaatcaata attaatctgg tatcgagtgg
     8341 cgcagttgac agccacacac cgcaaactgc cgttgctgcc gtgctgtgtg ccgcgatgat
     8401 tgggctgttt agctgaagct cgttgtttat gccggatgcg gagaaagtcg taggcctgat
     8461 aagacgcgac cagcgtcgca tcaggcatca tgctcagatg cctgatgcgg cgtaaacgcc
     8521 ttatccggcc tacaaaagca ataatttcaa caggttttgt tgcccagcgc atcaggcaat
     8581 cttgttctca gcacattgat ggcgctttcg ttttctactt tgtaaacgaa gcgccattca
     8641 ctctttaaat cacccgtgag aactgctgca tccgcgcttt ctggcgcaga taggtatcaa
     8701 agcacatgca aatgttgcgg atcagcaagc gacctttcgc cgtcacctgt attcccttct
     8761 catccacatc caccagccca tcttttgcta acggggcgag cagcttgaga tcttccgcaa
     8821 agtaatcagc gaagtgcaaa tcccactgtt tctcaagagg ggcgtaatcc agacggaagt
     8881 tgcagatgag cgacttaatc acatcgcggc gaatacagtc atcacgcgtt agcgcaatac
     8941 cacgccacag cgcattgcct tgttcatcca cttgctgata gtactgcttc aactctttct
     9001 ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga aacgcccatc cccagcagat
     9061 cggtatcgcc ctgagtggtg tagccctgga agttacgatg cagcacgcct tcacgctggg
     9121 ccaccgccag ctcgtcatca ggacgggcaa agtgatccat accgataaac tgatagcccg
     9181 attgcgtcag gaaggcgatg gtttcctgca ggatatcgag tttttgctgc ggactcggca
     9241 ggtcagcatc tttgattttg cgctgagcag caaaaatggt cggcagatgc gcgtagttaa
     9301 aaacgctcag acgatcgggg ttcagctccg ccacacgttt cagggtaaag gcgaaactct
     9361 ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt ggtggaggta aaaccaatct
     9421 cacgcgcatg gttaagcagt gcaaagatga actcttcatc ctgctcgcgg ttaaccagac
     9481 gttgcacttc tttgttgaag tcctgcacgc ccatgctcag gcgattaaag ccttcggcgc
     9541 gtaaatgatc gagtacatcc agttcgattt cccgcggatc gacttcgatc gaaatctccg
     9601 catcggtatt gaaatggaag ttttcgcgca gcagcttcat cagacggctg atttgcgctt
     9661 tattcagata cgtcggcgtt ccgccgcccc agtgcaattg gctgacgtga cgcccggcga
     9721 acagcggtgc acgatggacg atttcttgct ccagcgcgtc cagatactga tcggccttgt
     9781 gctgctggcg agtaacaatc ttattgcaac cgcagaagta acaaagctta tggcagaacg
     9841 ggatatgtac gtagagagat aatggacgct caggatagcg cgccacggct tgtaaaaacg
     9901 cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg gtacgaggtg tatcgtggcc
     9961 cggaatagtt atatttctgg atcagggcca gatcccagtc gatttgctgt acagacatgc
    10021 tcactccttc cgatggcgtc tctggcggct acggcgtacc ggcggatgcc cgtttcgggt
    10081 catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac cgctgtagtt taacgaataa
    10141 ccacaccaga taacatataa ccggaagggt tatcagcaga acgggaaggc ccacggcggc
    10201 aatccgttag ttgccccgca gcagacgcat catgtcttct tgcttctcgt cttcttcctc
    10261 ttcttcgtca tcgtcataag agaggccgag tttctgcatc aactcatcaa tacgatccag
    10321 tttagcatcc acccaggatt gctcttcggc gctcagggtt tcgcctgctt ccagacgttc
    10381 cagcagcgca tccagacgct catccgtttc cagtaattcc aactccgcct gcggtgaaag
    10441 cataggtttc tcactcttcg gtttgtgctg tttggtgact ttttcagtca ctcccaatgg
    10501 aataggggtt ttactgccaa tacgtggatc ttttggtgcg ttctggcctt tgctgcctga
    10561 cgtggtgttt ccgcccgctg cgcggctgcc cggcgcatga ccacgacgtt ttttctgacg
    10621 cttacggtca cgagcttcct gatccagctc ctcgcgtgtt tttcgacgcg cttttgcatg
    10681 acctttgctg cgtgagtttg aagatgatgg tttcataatg gttcgtcttt tagccgtttt
    10741 attcagtata gatttgcggc ggaatctaac agaaagcaag caaagaaaaa aggcgacaga
    10801 ttactctgtc gccttttttc ctgactcata acccttaacg ggtcttacaa tccttgcagg
    10861 ccgccaacac accctgtttt tccattagcc ggaacaatcc gtgttgattc catttccgtt
    10921 taattacgtc tccggacgtt tgtcttcctg acaatcctct gtcttcgcct cctggcgctc
    10981 ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc gctctatggg tgctacttta
    11041 cgcctttgct ttaaacaaac aatcaaccgc catcagtatt tgcacatttt cagagtttta
    11101 tataaaagca attcactgat ttatatagct tatttgacat gcatcgcaga attttctctg
    11161 ctatttcgca taaagactat cggcaatgtc tcacaaagcg tatggctttt acttacaacg
    11221 ctcacgccga aaaatccgcc ttttattatg gttcaggtat aatcgccaca aagctttgac
    11281 tgacggagac gaccgctttg actaatttga attatcaaca gacgcatttt gtgatgagtg
    11341 cgcctgatat tcgccaccta ccttccgata ccggaattga agtggctttt gcaggccgtt
    11401 ccaacgcagg taaatccagc gcgctgaaca cgctgactaa ccagaaaagc ctggctcgta
    11461 cctcaaaaac cccagggcgc acccagctta tcaacctgtt tgaagtggct gacggcaagc
    11521 gtctggttga cttgcctggg tacggttatg cggaagtccc ggaagagatg aagcgcaaat
    11581 ggcagcgtgc gctcggcgaa tacctcgaaa aacgtcagag cctgcaaggt ctggtggtgc
    11641 taatggatat tcgccatccg ctgaaagatt tggatcagca gatgattgag tgggcggtag
    11701 acagcaatat cgccgttctg gtgctgctga ccaaagcgga caaactggca agcggcgcac
    11761 gtaaagcgct attgaatatg gtgcgtgaag ctgtactggc gtttaacggt gatgtgcagg
    11821 ttgaaacgtt ttcttcgttg aagaaacaag gcgtagacaa gctgcggcag aaactggata
    11881 cctggtttag cgagatgcag cctgtagaag aaacgcagga cggcgaataa ttttcttgcc
    11941 ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat tcaatagatt acaattctac
    12001 cgttggcctg ataaacatag cgcatcaggc attacggatc ttttctttcg cccaataaaa
    12061 aacgccccag tcattactga ctggggcggc taaaatattc agccaaatcc gattacgtga
    12121 agtaaaaggt ctgaaagata gaacatctta cctctgtacc ctacgcgact aactttactc
    12181 ttttttgttc agttcagaaa gcactttttg taattttttt tcagttgttg cataggaatt
    12241 tctaatagcc atcacaaaac gtgacagctt atgttgctta cttacgaaaa aagggcatgt
    12301 tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac tccccacttc caccagcaac
    12361 ggcacatcca gacgggtaca gttttccatc agttgatgaa tctgcttcgc gacggcatca
    12421 acatcatctt tatgaacttc aaataccagt tcatcgtgta cctgcatgat catacgtaca
    12481 cgcggttgct cagcctgtaa ccacgcatca acggcaatca tcgcccgttt gataatgtcg
    12541 gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt cagccgctgc acgacgagca
    12601 ccattgctgg atttgatatc cggcagatac agacggcgtc cgtccagcgt ttcaacgtag
    12661 cccagctctt tcgcctgagc acgggtgcgt tccatatact ccagcacgcc agggtagcgt
    12721 tcgaagtaaa ggtccatgta cttctgcgct tctttacgtg gaatgttcaa ttgccgcgcc
    12781 agaccgaaag cactcatgcc ataaatcaga ccaaagttga tcgctttcgc gctacggcgt
    12841 tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt ctgccgccgt tgcccggtgg
    12901 atatcttttc cttccgcgaa tgcggtcagc aagcctttat cacgcgagag atgcgccata
    12961 atgcgcagtt caatctgcga gtagtccgct gagacaatca cataatcctc tggcgcaata
    13021 aacgcctggc ggatacgacg accttcttcg ttacgcaccg gaatgttttg caggttagga
    13081 tcggttgacg ataaacgtcc cgttgcagtt actgcctggt gataagaggt atgcacacgc
    13141 ccggttttcg ggttgatcat cagcggcagc ttgtcggtgt aagtcgactt cagcttcgcc
    13201 agaccacgat actccagaat cacttttggc aacggatagt ccagcgccag ttcttccagt
    13261 acctcttccg acgttgacgg cgcgccaccc ggcgttttct tcagcggttt aataccctgt
    13321 ttttcaaaca gaatggtttg caactgcttg gtggaagaaa ggttaaattc ttcacctgca
    13381 atttcatgcg ctttcttttc cagctcagcc agacgcaagg tgagctcttc agaatgattg
    13441 tgcagcactt tcggatcgat cttcacaccg ttacgttcaa tgcgtgaaag caccggcacc
    13501 agcggcattt cgatattctc gaagacgttc aacggccctt tgtgtttttg cagatccggc
    13561 cacattttca gatgcaactg caaggtgaca tctgcatctt cggcggcgta acgtccggcc
    13621 tcttcgaggg caatctggtt aaaggtcagt tgatttttgc ctttacccgc aatctcttca
    13681 aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc tgtccatatc gtgacgcccg
    13741 gcaacgctat tgagaatgta ggactccagc atggtatcaa acgcaatccc acgcagttca
    13801 atgccgtaat tcgccagaat accgcgatcg tatttcaggt tttgcccgac cttcagcgcc
    13861 ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac gctcgtgaga gatttgatcg
    13921 ggcgcatcaa gataatcatg agcaaccgga atatatgccg ctacgcctgg ctcgatagca
    13981 aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc tgtcggtttc ggtatcaaat
    14041 gcaaataccg gcgctttttc cagcttcgca atccacgctt tcagtgtttc ttcatcaagg
    14101 atagtgacgt agttgtcata agaaatcacc gttgccgtca cttctggcgc ttcgtctgca
    14161 acactggttt cctgcggctt cgcggctggt tttgcccctt tggcctgtaa ccatttgccc
    14221 gcttcgacat cagcagtcca gcgtttgaac tcatactttt tgaacagccc caacaactct
    14281 tccgctgccg gttgctgcac ttccagttgt tcacaggtca gctccagttc aacatcggtt
    14341 ttaatcgtcg ccaactggta tgagagataa gcaacttctt tgttttgctc gagcttcgct
    14401 gccattgttt tcgcgccacg gaagctcaac ccagcaattt tttctggctc ggtatacagc
    14461 gtatccagcc cgccaagacc ttgcagcaat gcctgcgcgg ttttttcacc gacgcccggt
    14521 acgccaggaa tgttatcaga ggagtcaccc atcagcgcca ggaagtcgat gatcaattct
    14581 ggcggcacgc cgtacttatt caccacctct tccggtccga ggatggtatt cgtcatggta
    14641 ttgataagcg taatatttgg cgtcaccagc tgcgccatat ctttatcgcc agtgctaatc
    14701 agcaccggac gcccggcttt ttcggcttcg cgcgccagag taccgataac gtcgtccgct
    14761 tctacgccag aaaccgccag cagcggcagt cccatcgctt taaccatcgc gtgcagaggt
    14821 tcgatttgtg ctcgcagatc gtccggcatt ggcgggcgat gtgatttgta atgttcaaac
    14881 agttcatcac gaaaggtttt tcccttggcg tcaaagacca ccgctgcatg cgtcggttta
    14941 tattgcatga tcagactgcg cagcatgttg aggacaccat acatcgcacc ggtcggctcg
    15001 cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc gataaagata agatgaacca
    15061 tctacaagga taagtggatt ttgggggatc tgaaccataa tgtccgtgcc tgtttatcag
    15121 attatgggta aaggatgcca cagacaggat gaaaacatga gtttttcacc gcttttcgtg
    15181 aaaatctttt gtagatctgc tggatcgctc gcaaatcgtc atgtggataa ctttgtgcat
    15241 aaattattta catcgctttt aatttattat catgcttttt acagcatgat gtttgtcttt
    15301 atttttcatg cagttaaaaa taaatccatc cctaataatt gagataatgg gattttttct
    15361 aatgtggata gatatgaatt atttttctcc ttaaggatca tccgttattt gggtcatttt
    15421 ctgctaaaat ctgcgccttg cgcttcttta gtgcactgtc tatggctaac cttttgaata
    15481 aattcattat gacgagaata ctcgctgcga taaccctttt gctgagtatc gtattgacca
    15541 ttttggtcac tattttttgt tccgtcccga tcatcattgc cgggattgta aaactattgc
    15601 tacctgtgcc agtcatttgg cgaaaggttt cgcgtttttg tgattttatg atgtattgct
    15661 ggtgtgaagg tctggcggta ttactgcatc tgaacccaca cctgcaatgg gaagttcacg
    15721 ggctggaagg gttaagtaag aagaactggt atctgcttat ttgtaaccac cgtagttggg
    15781 cagatattgt cgtactgtgc gtgctgtttc gtaagcacat tccgatgaat aaatatttcc
    15841 tcaagcaaca gctggcctgg gtgccatttc ttggcctggc gtgctgggcg ctggatatgc
    15901 cgtttatgaa gcgttattcc cgcgcttatt tattacgtca tccggagcga cgtggtcaag
    15961 atgttgaaac cactcggcgt tcttgtgaaa agtttcgcct gcatcccacc accattgtca
    16021 atttcgttga aggctcccgt ttcacgcaag aaaaacatca gcaaacccac tccacttttc
    16081 aaaacttgtt gccaccaaag gcggcaggca ttgcgatggc gctaaatgta ctgggtaaac
    16141 aattcgataa actgcttaat gttacgctgt gttatccgga caataaccgt cagccgttct
    16201 tcgatatgtt aagcggtaaa ctgacgcgga ttgtcgtaca tgtggattta cagcctattg
    16261 ccgatgagtt acatggcgac tacatcaatg acaaaagctt taagcgccat tttcagcaat
    16321 ggctgaactc attgtggcag gaaaaagacc ggcttctgac gtctctcata tcatcacagc
    16381 gtcagaataa gtaagtttta ctcgcctctg aatgagcaga ggcgagtgag tattttaatg
    16441 aacaaaacgt ccggcacgag acataaattc ttcttcgctc atctcctgtc cgttaaaaac
    16501 aactttaccc ggtgtataac gcagctgcag cgaggcggta ttgttgtcaa tcgtgattaa
    16561 ctgaaacatc tgacctaatg tcatcatccc gctgatttgt ttatcagcct gcttttgcgc
    16621 tttttccgca tccattcctt cagataaatt aagctgtttt gcggtttatg tgacgacatt
    16681 aagcggtaac ttcacatcaa aattgagcga tttgatatct ttgtttgtgg atgatgaaga
    16741 tttggctggg tcggcaatac tgatatccag attggcattt agttcgccga gtgcgttctt
    16801 ccatcttacc ggttgtttaa tggtcggctc acttttttgt aataacggta aatactcttt
    16861 gaacaatgca gcattcactt cttgcagagc aacttcatcg tttgctaact cagggtgtgc
    16921 agcaagctgc ttctgcatcg caatgttata ctggatgata aattggcgca ctgctgaggg
    16981 atcaatagat tcagcaatca atgaccactc accactaccg agttgctgat tcccttttgt
    17041 taacttatca agttcgtagg tcagattgac attgatataa tctttgtctt taacgcgatc
    17101 cagcctggtt tttgcaacga aagcatcgat ttgggcaacg tcttccccgt gattgataat
    17161 gttaatctga ttaaatttcg actcgctttc accaaccgga aatttctttt cttctaatcg
    17221 cgccagagaa tcaatggtaa aactttttgc cgttactttg ttcattggtg ataattgcag
    17281 aacaatatct tcgactttgc cttcaatatc caggttagac aatgaactac catcaccatt
    17341 aaaagtaaac tggccctcac caaaagaaaa ctttccatac tctgcagggg caacatctaa
    17401 tgtcgtagag aatgagccac caaacgcgaa tcgcaatgtc gcttccacgg gcgatttatt
    17461 tttcgcggcg ataaatagcg gttgtgtcag ttcattattc actacgttca cttttgccgc
    17521 tgccaatgct gggatgacat taccatgcat taacattgtg atgggcaacg gaccgtgctc
    17581 aacatccaca tcaaatacaa ctttctggcc tggcttgata ttgagatcgg gtgcaccgtt
    17641 atcgaatgtc atttgcattt gaatacggga attaaaaaag cctttgtcaa agtttttata
    17701 acttaacgtc atatcgtagg catgttcacc tttctttttc gcatcgttaa aatctttaat
    17761 aaacttttcg ataccaggct gaatttgcgt acctgtgtac catgtgccac caccccagat
    17821 tacggctaac gcaacaataa cacctgtagc tgacttacgt atcatataaa tattccctga
    17881 taaaacagca aaactttgaa tatccactta tgctgaaata atgtccagcg gcaggatgca
    17941 ttatcagaat gtatttatat ttattaaata ataaaaaaag cccgtgaata ttcacgggct
    18001 ttatgtaatt tacattgaat tattttttct cggacagata tttcaccgta tcagcatact
    18061 gctgaacaaa aacatccata ttgctggtat ccataccctg cggattcagc tgatatttac
    18121 cgttaacaaa catcgccgga acaccacgca attgcacatc agctgcggct ttttcctgct
    18181 gagcgaccag agatttcacc acgaagctgt tccacgccgc gtcgtactct tcacctttaa
    18241 tacctgcatt gataaataca tcgcggatat cagatgctga acgaatggtc tgggttttct
    18301 gtacgccttc aaacagcgga acagtcactt tgtcttccac gcccagcgcc atcgccacag
    18361 cccatgcctg agtcagatct ttgcccaggt caccacccat gaagttgacg tggtatttag
    18421 tcatcttcac gccttccggc agttttttct tcacattatc agaaatatgc agaacttctt
    18481 caaactgata gcagtgcggg cagaagaaag agaaaaactc cagcacttgc ggcgcgccag
    18541 ctaccggttt ttccagggta gtgtactgtt taccatcttc atactgcgcc gccgatgcgc
    18601 taaacgctaa aactaaacca gccagcgcca gccaaatctt tttcatgatc tactctctcc
    18661 gattaataca taggtgttaa ttgcaaaggg ggttcttgta gaacttttgc ctgttctata
    18721 aaagtcgccg tctgtcgtag ccagtaatct tccccggtta accacgggaa atttttcggg
    18781 aacgcgggat cagcccaatg ccgcattagc caggcaagat aataaaccaa acgcatggcg
    18841 cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact cgctaaattc ttcataagct
    18901 tcaataatag tttccagttg catccgctgc tgggctttat cgccattgag caacatccac
    18961 aaatcctgaa tagctggacc attacgtgca tcatccagat caacaaacat tggaccatcg
    19021 cgccagagaa tattcccggc gtggcagtct ccatgtagcc gcagaacggt gaaatcttcc
    19081 cgccagtgtg ctgtaacggc ggcgatcagc tcatctgtcg ctttcagaaa tgccgctttc
    19141 aacccggaag gtatcagtgt agcgtcctca aacagcttgc gaggctcaat gagatattca
    19201 ttcagaccga tggtctggcg atgaataaaa agctgtttgc gccccgtctg gtgcatacgc
    19261 cctaaataac gcccaaccgc ttccatctga tcgatattat cagcttcgaa ctggcgacca
    19321 ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat tcaataaagt ctgaccgtta
    19381 aaggccacag gtgctgcgac cggaacttca tcatttacca gctgcaacgc aaattgatgt
    19441 tcttcgagaa tttgatcggc tgtccaacgt tcagggcgat aaaatttgac gacaaaacgt
    19501 cgacgctctt cgtcctgaaa ttgatagaca cggttttcat agctgttaag cggggtaaga
    19561 ccggaatcca cccggatccc ttgctcaaac agagcgtcca tgatggtatc cgggtgtagt
    19621 gtctggaaag taaaagcgct gttgttcatc ccatcatccg gaaaacatta cgaatgatga
    19681 aggatatcat ttcacgatga ttttggtggc gccgcttaca agcttttact ctttaattac
    19741 gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca taatctttct gcaaacccgg
    19801 gatcaccgca tctttcgcgg aatcacgcat tttcaggtga tagatcagaa tgtcatccgt
    19861 caaatccgcc agttcgccgt caaaacctga ctctttcgcc agtttctgca aaaattgcaa
    19921 caagttaaag tctggctctt tttgccaggc tggctggagg agttcaataa cttcattcag
    19981 acgtttacat ttcatagtaa tgctccttac tcttgagaca gacacgttag cagggtcaat
    20041 cccacaataa aagaggcgat atcggtgaat ctgatgacga cgataacagg cgttgtgctg
    20101 gcaggcggta aagccagacg aatgggcggc gtagataaag gattgctgga cttaaatggc
    20161 aaaccgctgt ggcaacatgt cgctgacgcg cttaagacgc agctctctca cgtcgtggtt
    20221 aatgctaatc gtcatcagga aatctatcag gcaggcggtc tgaaagtgat tgaagattca
    20281 ctggcggatt acccaggccc tctggcagga atgctttcag taatgcagca ggaagctggt
    20341 gagtggtttt tgttttgtcc gtgcgatacg ccttacattc cccatgattt agccgcccgg
    20401 cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc atgacggtga acgcgatcac
    20461 ccgactattg ctctggtaaa ccgcgctatt gagcctttat tactggaata tctgcaagca
    20521 ggagaacgcc gggtaatggc atttatgcgt ctggctggcg gtcatgcggt tgatttcagc
    20581 gatcataaag atgcatttgt taacgtgaat acgccagagg agcttgcccg atggcaggaa
    20641 aaacgatgat accgttactc gcctttgccg cgtggagtgg taccggaaaa actacgcttt
    20701 tgaaaaaact gatcccggca ttatgcgcca gagggatccg tccggggctg attaagcata
    20761 cgcaccatga tatggatgtt gataagccag gcaaagatag ctatgagctg cgcaaggctg
    20821 gcgcggcaca aaccatcgtt gccagccagc aacgatgggc cttgatgacg gaaacaccag
    20881 acgaagaaga gctggatcta cattttctcg caagtcgaat ggatacctca aagctggatt
    20941 tgattctggt cgaagggttt aagcatgaag agatcgcaaa gattgtgctg tttcgcgatg
    21001 cagccggaca tcgacctgaa gaattagtga tagacaggca tgttattgct gtagccagtg
    21061 atgtgccgct taatcttgat gtcgcattac tggatattaa tgatgttgag gggctggctg
    21121 attttgtggt ggagtggatg caaaagcaga acggataagc aagagtaggc tggatcagac
    21181 gcagccagtg tcgcatccga cctttttctg caccagcgca aaaaggcccg actgggcct
//
LOCUS       DAJTYF010000065        21218 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTYF010000065 DAJTYF010000000
VERSION     DAJTYF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.065"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..76
                     /locus_tag="OO590_002473"
     CDS             <1..76
                     /locus_tag="OO590_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6637802.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            92..943
                     /locus_tag="OO590_002474"
                     /pseudo
     CDS             92..943
                     /locus_tag="OO590_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin"
     gene            1182..1241
                     /locus_tag="OO590_002475"
     CDS             1182..1241
                     /locus_tag="OO590_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6637803.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1558..1617
                     /gene="ibsE"
                     /locus_tag="OO590_002476"
     CDS             1558..1617
                     /gene="ibsE"
                     /locus_tag="OO590_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR6637804.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1737..3170)
                     /gene="hldE"
                     /locus_tag="OO590_002477"
     CDS             complement(1737..3170)
                     /gene="hldE"
                     /locus_tag="OO590_002477"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6637805.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3218..6058)
                     /gene="glnE"
                     /locus_tag="OO590_002478"
     CDS             complement(3218..6058)
                     /gene="glnE"
                     /locus_tag="OO590_002478"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6637806.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6081..7382)
                     /gene="ygiF"
                     /locus_tag="OO590_002479"
     CDS             complement(6081..7382)
                     /gene="ygiF"
                     /locus_tag="OO590_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6637807.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIVSAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7624..8244
                     /locus_tag="OO590_002480"
     CDS             7624..8244
                     /locus_tag="OO590_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6637808.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8308..9546
                     /gene="cca"
                     /locus_tag="OO590_002481"
     CDS             8308..9546
                     /gene="cca"
                     /locus_tag="OO590_002481"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6637809.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9709..10530)
                     /gene="bacA"
                     /locus_tag="OO590_002482"
     CDS             complement(9709..10530)
                     /gene="bacA"
                     /locus_tag="OO590_002482"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6637810.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10621..10989)
                     /gene="folB"
                     /locus_tag="OO590_002483"
     CDS             complement(10621..10989)
                     /gene="folB"
                     /locus_tag="OO590_002483"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6637811.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11094..11711
                     /gene="plsY"
                     /locus_tag="OO590_002484"
     CDS             11094..11711
                     /gene="plsY"
                     /locus_tag="OO590_002484"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6637812.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11724..12655)
                     /gene="ttdR"
                     /locus_tag="OO590_002485"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11724..12655)
                     /gene="ttdR"
                     /locus_tag="OO590_002485"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12862..13705
                     /gene="ttdA"
                     /locus_tag="OO590_002486"
                     /pseudo
     CDS             12862..13705
                     /gene="ttdA"
                     /locus_tag="OO590_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13702..14307
                     /gene="ttdB"
                     /locus_tag="OO590_002487"
     CDS             13702..14307
                     /gene="ttdB"
                     /locus_tag="OO590_002487"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6637813.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14355..15752
                     /gene="ttdT"
                     /locus_tag="OO590_002488"
     CDS             14355..15752
                     /gene="ttdT"
                     /locus_tag="OO590_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6637814.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALMLLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNN
                     TGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRYFFASATAYTSALAPMMIAAALA
                     MPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLV
                     LLVITGLLWMPVVLL"
     gene            complement(15795..16808)
                     /gene="tsaD"
                     /locus_tag="OO590_002489"
     CDS             complement(15795..16808)
                     /gene="tsaD"
                     /locus_tag="OO590_002489"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6637815.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17046..17261
                     /gene="rpsU"
                     /locus_tag="OO590_002490"
     CDS             17046..17261
                     /gene="rpsU"
                     /locus_tag="OO590_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6637816.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17372..19117
                     /gene="dnaG"
                     /locus_tag="OO590_002491"
     CDS             17372..19117
                     /gene="dnaG"
                     /locus_tag="OO590_002491"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6637817.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19312..21153
                     /gene="rpoD"
                     /locus_tag="OO590_002492"
     CDS             19312..21153
                     /gene="rpoD"
                     /locus_tag="OO590_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6637818.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5119 a         5865 c         5442 g         4792 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac cgaacaacaa gaagcgttta tgacgcttga
      121 gcaggagcag caggttaaaa cccgtactgc cgaacagaat gcacgtattg cggcttttga
      181 agctgaacgt cgtcgtgaag cagagcagac gcgaattctg gctgaacgac aaattcagga
      241 aacggagatc gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg aacgtgaagt
      301 tcgtattaaa gagatcgaac agcagcaggt caccgaaatc gccaaccaga cgaaatcgat
      361 cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa gccgatcgtg ctaaacaagt
      421 tgccctaatc gctgccgcgc aggatgcaga aaccaaagcg gttgaactga ccgtgcgggc
      481 gaaagcagaa aaagaagccg cagagatgca ggcggcggct atcgttgagt tagccgaagc
      541 tacacgtaaa aagggtctgg cggaagcaga agcacaacgt gcgctgaacg atgctatcaa
      601 cgtactttct gatgaacaaa ccagccttaa attcaaactg gccttgttgc aggcgctacc
      661 tgcggtaata gaaaaatccg ttgagccgat gaaatctatt gacggtatca agattattca
      721 ggtcgatggt ctgaatcgtg gcagcgctgc gggtgatgca aacacgggta atgtgggggg
      781 cggaaaccta gcggaacaag cattatcagc cgctctctct taccgcacac aggctccgct
      841 gattgactcc ttgctcaatg aaattggcgt ttcaggcggt tcactggcgg cattgacttc
      901 acccttaacc tcaacaactc ccgtcgccga aaacgtagaa taaattttca gctatgaacg
      961 tccctggcac aaaaagccgg ggatttttta tatctgcgtt ccgctaaaaa ctgcaaatga
     1021 tcaggtcgtt gcaacgtttt gcgtgaccgc tcggggaagg caaaactgcc tctgggaaag
     1081 cgttgcgcga ggtccggcgc tcatcaacaa tcggggagtc agcaaagggc tgaaacggga
     1141 aagcccctcc cgaggaaggg gccttacaga aggaaagggt tatgatgaag ctcgtcatca
     1201 tactgattgt gttgttactc gtaagtttcg cagcttatta acagccaatc agagggggga
     1261 gaaatcctcc ctcacccttg ttcctttact ttaggttgaa aaaaaatcag cgtcaagaag
     1321 gtttcattaa cacatatccc tggcccaaaa agccggggat tttttatatc tgcgttccgc
     1381 taaaaggtgc aaatgctcag gacgttgcag cgttttgcgt gaccgctcgg ggaaggcaaa
     1441 actgcttcag ggaaagcgtt gcgcggggtc cggcgctcat caacaatcgg ggagtcagca
     1501 aggggctgaa acgggaaagc ccctcccgag gaaggggcct tatagaagga aagggttatg
     1561 atgaagttcg tcatcatact ggttgtgttg ttactgttaa gtttcccgac ttactaacaa
     1621 ctgatcagag gggggagaaa tcctccctta cccttgttcc tttactttag gttgaaaaaa
     1681 caacagcgtc aataggcctg cctgctacga agcgagatct gtgaaccgct ttccggttag
     1741 ccttttttat cctgttggat cttcttgatg atgttagtgg tcgagcaacc gtcttcgaag
     1801 ttgagcacca gcacttcgcc gccgttggcc cagacttctt tactcccggc gatctcttct
     1861 ggtttatagt cgccgccctt caccagtaaa tctggcagga tcccggcgat caagcgctgc
     1921 ggcgtgtctt cttcaaacga caccacccag tcgaccgctt ccagtgcgcc cagcacaatc
     1981 atacgctgtt cgagcgggtt aacggggcgg gaatcccctt tcagccgttt ggtggaggca
     2041 tcgctgttca cagcgacaat caagcggtca cccagtttgc gggcatttgc cagatagaag
     2101 acgtgtccgg cgtgcaggat gtcaaagacg ccattggtca tcaccacttt ttcaccgcgt
     2161 ttacgcgctg ccgctacggc cagcttcagt tcctcttcgg tcatcacgcc aaagccggta
     2221 tctgcgcgcc cacgcactgc attttccagc tcgatcggcg aaaccgtgga ggttcccagt
     2281 ttgccgacca ccacgccagc cgccgcattg gcaaagaagc aggcttcttc cagcgaatta
     2341 cccgctgcca gcgttgccgc caggacgcca atcaccgtgt cgcccgcacc ggtaacgtca
     2401 tacacttcct gcgcctgggt tggcatatgc agcggcgctt tacccggttg cagcagcgac
     2461 ataccctgtt cggaacgggt cactaacaga gccgagagtt cgtaatcggc aatcagtttc
     2521 atgccgcgct caacaatctc ttcttcggtc ttacatttac cgacaacagc ttcaaattcc
     2581 gagagattcg gtgtcagcag cgtagcgccg cggtagcgct caaaatcggt accttttgga
     2641 tcaatcagca ccggaacgcc cgctttacgc gccagttgga tcatctgctg tacgcttgcc
     2701 agcgcacctt tggcgtagtc agaaagcacc agcgcgccaa tcgaactcag cgcctgatta
     2761 atccgctcgt gcagcggctg cggatcaaca ccttcgaaac cttcttcaaa atccagacgg
     2821 atcagctgtt ggttgcggga aagtacccgt aatttggtga tggtcggatg cgtcggtaca
     2881 gaaacgaagt cgcatttgac gttgacgtcg gccagagatt tactcagcgc gcgcgctgca
     2941 tcgtcaatgc ccgtcaaccc gaccaggcgt gcattagcac cgagagaagc gatattcatc
     3001 gccacgttag ccgcgccgcc cggacgttct tcgatggtat tcactttaac cacgggcacc
     3061 ggcgcttccg gcgagatacg actggtgggg ccgtaccagt aacgatccag catcacatca
     3121 ccaaccacca tcactcctgc acgttcaaac tctggcagcg ttactttcat tcctgtctcc
     3181 tgagagattc aaaatttgcg cgcgataata ccatacttca ttcttccacc agccacttct
     3241 gccagcttgc ccgcaccaga tcacgctctg cggtgaagca atcctccgac acatggcccg
     3301 gcaattcctg taatgccaga tgatgaagtt catcgcgcag cgtcgtgtaa gcacgggtca
     3361 gcgccatcgc ttcctgctct tccataatgt cgttttgcgc cagcagctcc agaatacgca
     3421 cgttgtctga ccagcgcgtt aacttcggtt tttcatgagc gtagcgcaac accagatatt
     3481 gggtaataaa ttcgatatcg gtaattccgc cttcatcggc tttgatatca aagcgattac
     3541 gatgtttatt gccgagatga gcgcgcattt tctcgcgcat ttcccgcact tccgtttgca
     3601 gagttttacc ttctcgcggc agtgtcataa tctcacgacg caccgcgtca aagtgcgcag
     3661 tgagctgcgg atcgccgtac actacacgcg cacgcaccag cgcctgatgt tcccacgtcc
     3721 aggcctcgtt tttctgataa tcggcaaatg ctgctgcgga tgtcaccagc attcccgccg
     3781 ccccggacgg acgcagtcga gcatccactt catacaaaat gccggaagag gtacgcgtac
     3841 tgaacagatg cataatgcgt tgcgccagac gcagataaaa ctgccgcccg tcgatttccc
     3901 gctcgccgtc agtcatcgca tccattgggc aatcatggag gaagataagg tcaagatcgg
     3961 aactgtagcc taactcccag ccgcccagct tgccgtagcc gaccaccgca aaaccgcgcc
     4021 cttcacgttc gttgaggtga tttggcttac cgtagcgggc caccatttga ccccacgcct
     4081 gctgaacgac ggcatctatc atagcttctg ccagccaggt taagtgatcg ctcactttca
     4141 ttaccggtag cgtaccggcg atatccgctg ccgcaatgcg taacagctgc gcctgtttga
     4201 actgacgcag cgcctcaagc tgttgttctt cgtcatcttc cggcacgcgc agcaaatact
     4261 ggcgcagttc atcacggtag gcatcggtcg ccgtcggctg gtaaagggta tttggatcga
     4321 gcaattcatc cagcaataat ggataacgcg ctagctggct ggcaatcatc ggcgatgcgg
     4381 cacacagaga aatcaaatgt ttaagcgccg cagggaattc actgagcaat tctaaatagg
     4441 ttgtgcgggt aacaatcccc accagcaagg cggtaatgcg cgacagcgtg acagcagcgt
     4501 cttcacgcgc acagacatca cttagcagat gtggcatcag atggtcgagc acctgacgtc
     4561 ctcgcggccc gatagtacgc ttatccagct ctttgcggaa atcagcaatc agcgttagga
     4621 cctgtttgcg atcatcctcg ctaagatgcg ccagcactgg cgtagtgtca tcttcctgca
     4681 acgcatcctg ccacagctca cgccactgtt ccgacagcga ctcttcctga gtttcacttt
     4741 cgtcgtcgcc aatcaattca ttaaacaccc ggcgcacatt ggtcatatgt gcggtcagcg
     4801 ccccggtcag ttgcggccag tcagcaaagt ccatcgccca cgccagccgc gcacggttaa
     4861 gctcatcaga aggaagcgtt tgggtttgtt cgtcgttaat gctttgcagc aggttttcca
     4921 gacgccgcag gaacagatat gccactcgca attgttcagc atcgttttca gaaagcagat
     4981 gcagcgcggc aatggcgctg agcgttggca gtaaagagcg cgactgcagc aacggttcgc
     5041 gcccaccgcg aatgagctgg aacacctgaa cgataaattc aatttcgcga atgccgcctg
     5101 cgccgagttt gatattgtcg gtcaaaccac gtcgacgcac ttcacgggca atcatccctt
     5161 tcatgttgcg cagcgactga atcacgctga aatcgatgta acgacggaaa acaaacgggc
     5221 gcagcatcgc acgcaactcg ttagcataga cgccttcgct atcgcccata atccgcgctt
     5281 tgaccatcgc gtaacgctcc cagtcgcgtc cctgctcctg gtaataatcc tccaacgcgg
     5341 caaagctcag caccagcggg ccactttcgc caaacggacg cagtcgcata tccacgcgat
     5401 agacgaagcc atcctgcgtt ggttgatcca gcactttaat cagccgctgc cccatgcggg
     5461 taaaaaactg cgcgttatcc agttcccggc gtccgccctg cgtacaacca tgttccggcc
     5521 aggcaaaaat cagatcgata tcagaggaga aattcagttc cccgccgccc agcttaccca
     5581 tgcctaagat cagcagcggt tgcgcttcgc cctgcgcatt gcacggcgtt ccccactcgc
     5641 ggcagcaggc gtcatacagc cagtcacgcg ccgcaacaat cagcgtctcc gccagataac
     5701 tgagctgctg caatatgctc tcttcagtaa ccagtgccag cgtttgcgcc catgcgatgc
     5761 gcaccataat gcgccgccgg aatagccgca gctcgcgcat taacccggct tcgtcgctca
     5821 cattactgag cgcctcctgc aaccacacgc tgtaatgttg ccattcgtca gcctgtggcg
     5881 gttggctttc cagttccgtc agccactctg gatgcgcaat cacgctgtcc tgcacaaaat
     5941 cactgaaagt aagtactgac ttcgcctgtg cactaagtga ttcctcggct aaaagctctg
     6001 gcagccgctc aacaacggtc tgccagtact gctgtaacgg tgaagagagc ggcttcataa
     6061 gcgattttat ccttgcctga ttaacgtttt ccgctgtgca accagaacgg ttcctgattg
     6121 tttgcctcat tacggaaatg ttcaatttca atgcgttgcc cggtcgcaat agcgtgacgc
     6181 agcccctgcc agttctccag ccaggcttgc gcgacgacag gatcatagta acccgccagc
     6241 aacagtattg agtcaatatc acgcatcagg cgtggcaact ggtcacggta gcgatcgcct
     6301 aacggctggc agaaaacgct tttcagttca gcggcatggc gggaaagatg gatatcggca
     6361 aagcgtttga aggagtcgct aattttgccc tgcgctttgg catctaaaaa cggctgccat
     6421 gctttgctta ccagccattc ggtcaacgcc agcttcgcca ttgccgtttc ggtagagtag
     6481 accgccgtca cggcagaaac cgcagaaacg atggtcgctt cgcattgagt cagcaaatca
     6541 cgtaagtgag tgctcgcttt acgcggcaca atgccaccga acagcatcag tgtatgacgg
     6601 actaggctaa tggctgccag cacctgctct ttcgccgcat cgttgccgcg tacccacagt
     6661 tcttcatgat actgccattg cgctaacacc agctcgagcg ccgcttccag cccctgttcc
     6721 acatcggctt ttgccgcaac atgcaaaatg gtggtcggtt tgatttcacg cgccggattg
     6781 ccctgcgcca gatgatagcc acgcgccgct ttgctcaggc tgccctggcg taatccggtt
     6841 tgcgatacca gttggttcgc cagtttcagc accgcgcgcg tgtcgccgct aagcagctcc
     6901 agttccagct cgcagatagg ctcagcaaat tcacccgctt tcacttcccc ctggtcgaga
     6961 gcgatttcaa ttcggctgcc atcgatctct actagccatt tttcgcgata aaaatcggtg
     7021 ctaaacagcg gctgcacgcg ggaagcgaga tcggcaggca attcgccgtt cggccagatt
     7081 tccgtcggta actgcgccag gtcgagcgtc ggttcgctca acgccacgtt atattccggg
     7141 cgctggtgta agccgcctgt cactcttcct gcaactttca tggtcatctc atagcgaccg
     7201 ttttcgccac gaatacgtaa gcccatatcg tgcctacgca gccagttatc cggcgtttcg
     7261 tagtaaatat tcagcaactg cacggggtca tggtgctcgc cgcccagcgt attgagatgg
     7321 tcacgcaacg cctcaacggc actgtgatta acaataaact ttaattcgat ttcctgagcc
     7381 atggccttta cttatcggtt atgtcacata agtgacgatg aacggcgaac ttaatgcgat
     7441 cttttttgtc agtagatagt attttgcgcc aaattgccat gcaacgggca atttgacggg
     7501 cgtaaaagtt tgaagcagtg gcaagacctg acacagagga ttccgattga tgtcgaatgc
     7561 tttgcgtagc gacactgatg ccactactat cgttccactt tccatgacaa taacgacagc
     7621 ctgatgccaa aattacgcct gatcggatta actttactcg cacttagcgc gactgccgtc
     7681 tcacacgctg aagaaacgcg ctatgtttct gacgaactga atacctgggt ccgtagcggt
     7741 ccgggagatc attatcgcct cgtgggcacg gttaacgccg gcgaggaagt gaccttatta
     7801 caaactgacg ccaacaccaa ttatgcccag gtgaaagaca gctctggccg taccgcctgg
     7861 atcccgttga aacaacttag cactgagcca agcctgcgct cccgtgtgcc agatctggaa
     7921 aatcaggtca aaaccctgac cgataaactc accaatatcg ataacacctg gaatcagcgc
     7981 accgcagaaa tgcagcaaaa agtggcgcag agcgacagcg tgatcaacgg gttaaaagaa
     8041 gaaaatcaga aactgaaaaa cgagctgatt gtcgcgcaga aaaaggtcga tgccgccagc
     8101 gtacagctgg atgacaaaca gcgcaccatc atcatgcaat ggtttatgta tggcggtggc
     8161 gtgctggggc ttggcttgct gctcggtctg gtactgccgc acctgatccc aagccgcaaa
     8221 cgcaaagatc gctggatgaa ctaaatcgcc ttcttcgttg cactgtctta ttattaggaa
     8281 ttgtgtattt gacgaaggga tgatggcgtg aagatttatc tggtcggtgg tgctgttcgg
     8341 gatgcattgt tagggctatc ggtcaaagac agagattggg tggtggtcgg cagtacgcca
     8401 caggagatgc tcgacgcggg ctaccagcag gtaggccgcg attttcctgt gtttctgcat
     8461 ccgcaaacgc atgaagagta tgcgctggca cgtaccgaac ggaaatccgg ttccggttac
     8521 accggtttta cttgctatgc cgcaccggat gtcacgctgg aagatgatct taagcgtcgc
     8581 gatctgacca ttaatgcgct ggcccaggac gataacggcg agattatcga cccgtacaac
     8641 ggtctgggcg atctgcaaaa tcgtctgttg cgccatgttt cccccgcttt tggcgaagat
     8701 ccgttacgcg tactgcgcgt ggcgcgtttt gctgcgcgtt atgcccacct cggttttcgt
     8761 attgccgatg aaactctgac gctgatgcgc gagatgaccc atgcgggtga actggaacac
     8821 ctgacgcctg aacgggtatg gaaagagacg gaaagcgccc tcaccacccg caatccccag
     8881 gtgttcttcc aggtactgcg cgattgcggc gcacttcgcg ttttattccc ggaaattgac
     8941 gcactgtttg gcgttccggc acctgccaag tggcatccgg aaatcgacac gggtattcat
     9001 accttaatga cgctctcaat ggcggcgatg ctgagtccgc aggttgatgt ccgtttcgca
     9061 actttatgcc acgatctcgg taaaggactg acgccgccag aactttggcc gcgtcatcat
     9121 gggcatggcc cggcgggtgt taagttagtg gaacaactat gccagcgtct gcgcgtgcca
     9181 aatgaaattc gcgatttagc cagactggtg gctgagtttc acgatctcat ccacaccttc
     9241 ccaatgctga acccaaaaac catcgtcaaa ttatttgatt ccatcgatgc ctggcgtaaa
     9301 ccgcagcgtg tcgagcaact ggcgctgacc agcgaggctg acgtgcgcgg cagaaccggt
     9361 tttgaatcag cggactaccc gcaaggccgt tggttgcgcg aaggctggga agtggcgcag
     9421 tcagtgccga caaaagcggt cgttgaagcg ggatttaaag gcgtggagat tcgcgaggag
     9481 ctgacccgac ggcggattgc ggcggtagcc agctggaagg aacaacgttg cccaaagcct
     9541 gactgagtat aaaactaaac gttagcacga accgtcccct cgccccttcg gggagagggt
     9601 tagggtgagg ggaacagacc agcaccggtg cgaacaatca cccccacccc ggccctctcc
     9661 ctacagagcg agggagaaaa cccggcctgc tcccgaacct tttctcaatc aaaagaacac
     9721 gacatacacc gcagccgcca caataaagcg ataaatggcg aacgggataa acgaaatgcg
     9781 cttaatcaat tgcaggaagg ttttaatcgc tatcagcgcc accacaaaag cggtgataaa
     9841 cccaacggca aacatcggga tatcgccggt tgtcaggaag ccccagcttt tgtagagatc
     9901 gagcgccgtt gcgcccatca tcatcggtac cgccagcagg aacgagaact cggaagcagc
     9961 gtaacggctc acgcccatca gcatcccacc tgaaatggtc gccccggaac gggagaaacc
    10021 cggccacagc gccagacact ggaaacagcc aatcataaac gcctgacgat aggtcatatc
    10081 atcaagaccc ggcgcacgcg gctctttcgg cttcaggcat tcggcggcaa tcagcaacaa
    10141 accgccaacg accagcgcat acatcacatt tatcgggtta aacaatgact taatcgtgtc
    10201 gtggaataat agccccagta ccaccgccgg aatcatcccc agcaaaatgt ggatcagcgt
    10261 taaacgacct ttgctttcac cttcgtgctg caacgggcgg ccaaagtgga tgccaatcag
    10321 gccaaacaga cgccgccaga acatcactac taccgccaga attgatccta actggatcac
    10381 aacttcaaag gttttcgccg tgtcgccctc aaaccccaac aagtgaccga caataatcat
    10441 atggcccgtg ctggataccg gcagaaattc tgtcaatcct tcgaccacac ccaatattgc
    10501 cgctatcagc agcgagtgca tatcgctcat caataaaccc ctaaattatt aaaatgtacc
    10561 gcttgtccga actactgcag tatgaccagg ttataaccgt ttggtttaac agctgtaaaa
    10621 ttaattattt tctttcagat tattgccacg ctcaatgatt acgccaacat tcgccgcccg
    10681 cgccactgcg cctggcttgc tgagtttgat acgcacccac ggcgagttga agcgtgctaa
    10741 cagcagctcc gccacctctt cagccacgcg ttccaccagc gcaaaacgcg ccccctcgac
    10801 gtggctgacc accgtttctg caatgtcagc gtaactgagg caatccgcca catcatcact
    10861 tttcgccgct ttacggttat cccatgccat ttcgatatcg aacactaact tctgttcgat
    10921 ggtctgttcc cagtcgtaaa caccaatagt ggtgattacc gaaagttgct ctataaatac
    10981 aatatccatc acgtcctgcc tgctttttgg ctaaccggat accacttccg gcgaaatgtg
    11041 cgtattatcc acagattcat cgttgaacac gaattttcaa aacggaacag cttatgagtg
    11101 caatcgcgcc tggaatgatc ctcatcgcgt acctctgcgg ctccatttcc agtgccattc
    11161 tggtttgccg cttgtgtggg ctgcccgatc cgcgaaccag cggctccggc aatccaggcg
    11221 caaccaatgt gttacgtatc ggtggcaagg gagcagccgt agcagtactg attttcgacg
    11281 ttctgaaagg aatgttgcct gtctggggcg cgtatgaatt aggtgtcagc cccttctggc
    11341 taggcttaat tgccatcgcc gcctgtcttg gacacatctg gcccgttttc ttcggtttta
    11401 aaggaggaaa aggcgttgct accgcttttg gagcaatcgc accaattggc tgggatctca
    11461 ccggagtaat ggcgggaacc tggttactga ccgtgctatt gagcggatac tcgtcgctgg
    11521 gagcgattgt cagtgcactg attgctccgt tttatgtctg gtggtttaag ccacaattca
    11581 ccttcccggt ttcgatgctc tcttgcctga tcctgctgcg tcatcatgac aacatccagc
    11641 gtctgtggcg tcgtcaggag acaaaaatct ggacgaaatt caaaagaaag cgcgaaaagg
    11701 atcccgagtg atttctggtg gatctacatg acctgatagc cttcatcggg cttgcccagc
    11761 cgttgctggc accatgccgc cagaaattcc acgcagacac gaatttcatg ctgcgataga
    11821 gcggctcccg gtaaacagcc cagatattgg cgctttgtgc atactccggc aatacttgca
    11881 ccagtttgcc actctccaga aacggcaaca catcccactc ggaacgcagc ataatccctt
    11941 tgccctccag cgcccattgc aacactatct cgccgctatt ggaggaaaga tgcccgctaa
    12001 ccttcaccga ttttttctcc tgaccgttcc ccaactccca tattccgtgg gtcatatcgc
    12061 gctctttggt caccagacag tcatgacgac ttaattcctg taatgattgt ggctgcggat
    12121 atttttgcag atactcaggt gctgcgcata atattctttt atttttcgtt aacagatgcg
    12181 caatataata atcaggaatt tcgtcattaa tacgaatatc cagatcaata ttatcctgca
    12241 ctaaatcaat ttgccgatcg aacaattcaa aatgcacctg taactcagga taattgcgca
    12301 taagttcggt aatggctggc gcaatatggc tgcgcccaaa accaaaactg caaccaatac
    12361 gaatcatccc ctccgggcgc gttttgattt gcgtgacgtc atcgaccagt cgctgatatt
    12421 gggtgaggat ttccagcgca tgttcgtaac aacgttggcc actttcagtc agcgccacgc
    12481 cgcgagcgga gcggttcagc agcgttgttg ccagcgtatt ttcaaggatt tggatccgct
    12541 tggtgacaaa tgcaggcgtt tgcccaagcg tcgccgctgc agcactaaaa ctcccgctgt
    12601 ggacgatttc taccagtacc tgcaagtctt tggctaaagg ccagctattc agcatcgtta
    12661 attatccgca gttgtgataa gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg
    12721 gcgcaatatt cattcttatt ttcgcgttga ttcacgaact gttaattaca tattcacggt
    12781 ggcaaaaaat ataaaaccac atttttagtg gtagtttgtg gcggtgaatt tttccagaca
    12841 aatacaaaaa ctggagttgc catgatgagc gaaagtaata agcaacaggc agtgaataag
    12901 ttgacagaga ttgtcgctaa ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg
    12961 gtggataaac taaaacagct aaaggatgcc gaaacgtcgt cgatggggaa aattatctac
    13021 catacgatgt tcgacaacat gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac
    13081 accggggaga ttatgttctt cgttaaagtc ggttcccgct tcccactgct tggcgagctg
    13141 caaagcatac tcaaacaagc cgtggaagag gcaaccgtca aagcgccact acgtcacaat
    13201 gcggtagaaa tttttgacga agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg
    13261 gtcacctggg acatcatccc cgacaatgac gatgcggaaa tcgaagttta catggcaggc
    13321 ggcggctgca cgctacctgg ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc
    13381 gtggtgaaat tcgtcttcga aaatatctcc accctcgccg taaacgcctg tccaccggta
    13441 ctggtgggcg tgggcatcgc cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc
    13501 attctgcgcc cgattggcag ccgccatccc aatccaaaag cggcagaact ggagctacgc
    13561 ctggaagaag gactcaaccg tctggggatt ggtccacaag ggctgaccgg caacagttca
    13621 gtgatgggcg catcgtcgcg gcacgctgct ggttcatgcc gatctcacct ttgaaaatct
    13681 gtctcacacc cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat
    13741 ctgcaagata ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgc
    13801 gacgtttgtc accgccgttt gatcgaactg aaacgtccga tcccttacga tctcaacggc
    13861 aaagcgattt tccacgctgg ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtc
    13921 tccgtcggcc cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tattgagcag
    13981 accggcgtaa aactggtggt tggcaaaggt ggtatggggc cgctgaccga agaaggctgc
    14041 cagaaattca aggcgttaca tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc
    14101 caggtagaag agattgaaga agtgcactgg acagaactcg ggatgccgga gtcactgtgg
    14161 gtctgccggg tcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaac
    14221 ctgatagccg aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc
    14281 tgcgagcatg tccattacat caaataaccc tcccggagag gctcacccct ctcctttttc
    14341 gcaggcataa cacgatgaaa ccttccactg aatggtggcg atatcttgca ccgctggcgg
    14401 tcatcgccat tattgctcta cttccggttc ccgcagggct ggagaaccat acctggctct
    14461 actttgccgt ttttactggc gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg
    14521 tggtggcgat ggtgggtatc tccatcatcg ccatactctc tccctggctg ctgttcagcc
    14581 ccgaacagct cgcccagcca ggctttaagt tcaccgccaa atccctctcc tgggcagttt
    14641 ctggtttttc taattcggtt atctggctca ttttcgccgc ctttatgttt ggcacaggct
    14701 atgaaaaaac cgggcttgga cgccgcattg cgctgattct ggtgaaaaag atggggcatc
    14761 gcacgctgtt tctcggctat gcggtgatgt tctccgagct gatcctggca cctgtaacac
    14821 cgtccaactc ggcgcgtggt gcgggtatta tctatcccat catccgtaac ctgccaccgc
    14881 tctatcaatc acaaccaaac gacagcagtt cgcgcagcat tggctcgtac atcatgtgga
    14941 tggggattgt tgccgactgc gtgaccagcg ccattttcct gacggcgatg gcacctaact
    15001 tgctgttaat tggactgatg aaaagcgcat ctcacgccac gctgagttgg ggcgactggt
    15061 tcctcgggat gttgccgctc agcattttac tgcttctgct ggttccctgg ctggcttacg
    15121 tgctataccc gccggtactg aagtctggtg atcaggtgcc gcgctgggca gagacggaac
    15181 tgcaggcaat gggcccgctc tgttcgcgtg aaaaacggat gctggggctg atggtaggcg
    15241 cgctgatgct gcttctgcgc attatcagtt gggacgacat tgtcagtaat aaagcggcat
    15301 ggaacgtttt cttctggctg gcctcgctta tcaccctcgc gaccggactc aacaataccg
    15361 gttttattag ctggtttggc aaactgttag caggcagctt aagtggttat tcgccgacga
    15421 tggtgatggt gacgttgatt gtggtgtttt atctactgcg ctactttttc gccagcgcca
    15481 cggcgtatac ctccgccctc gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa
    15541 tcccgctgcc ggtgttctgc ctgatggttg gcgcggcaat tggtctgggg agcattctta
    15601 caccatacgc caccggaccc agtccgattt actacggtag tggttatctg ccaacggcgg
    15661 attactggcg actgggggcg atttttgggc tgatattcct cgtgttgctg gtgattaccg
    15721 gcttactgtg gatgcccgtg gtgttgcttt aagtgaggac ggtgccggaa attgctctcc
    15781 ggcacccaat agttttacgc agccggtaac tccgccagcg gccagcgcgg acgcacgcta
    15841 acgccgagat ccgccgtcgc gcctgcttta aaccgcacca ttccggcata ggcgatcatc
    15901 gcgccgttat cagtacaaaa ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc
    15961 atcatttcag ccagcttcgc ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc
    16021 agtcgcttaa agcccgtctg atccaacgct cgcttacact taatcatcaa cgtatcgacc
    16081 accgcatctt caaaggcgcg ggcgatatca gcacgcgtct ggtcgtcggt gctgttgtca
    16141 cgaatcgtgt tcgccgcaaa ggttttcaga ccagaaaagc tgaaatccag ccccggacgg
    16201 tcggtcatcg gacgcgggaa aacaaagcgc ccggcagtac cctgagccgc cattttcgac
    16261 agtaacggtc cgccaggata atccagcccc agcagcttcg cggttttatc aaacgcttca
    16321 ccggcggcat catcgataga ctcgccgagc agctcgtact gaccaatgcc agtcacgctg
    16381 attaactgcg tatgaccgcc ggacaccagc agcgcgacaa acggaaattc cggcgggtta
    16441 tcttccagca tcggcgctaa cagatgccct tccatatggt gtaccgggat tgccggaacg
    16501 ttccaggcaa acgccagaga gcgccccacg gttgcgccaa ccagcagtgc gccgactaat
    16561 ccagggcctg cggtataggc cacagcatca atgtcttttg ccgttaaacc agactccttt
    16621 agcgccgcct ggatcaacgg tacggtttta cgcacatgat cgcgggaggc cagttcaggc
    16681 acgacgccgc cgtagtcagc gtgcaatttc acctgactat acaattggtt ggctaacaaa
    16741 cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc aggaagtttc aatacccagt
    16801 acacgcatga cttgttttac ctcgctttat taccgcgcag tgtaggacca atgcgggttg
    16861 atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat actcctcacc cttataaaag
    16921 tccctttcaa aaaaggccgc ggtgctttac aaagcagcag caattgcagt aaaattccgc
    16981 accattttga aataagctgg cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga
    17041 ggcacatgcc ggtaattaaa gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct
    17101 tcaagcgttc ctgcgaaaaa gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg
    17161 aaaaaccgac taccgaacgt aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga
    17221 aactggctcg cgaaaacgca cgccgcactc gtctgtacta attccccgag agcgttgctc
    17281 tccgatcaga ccgagttgta gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt
    17341 ttcgtttatg aattgctaaa aatcggggcc tatggctgga cgaatcccac gcgtattcat
    17401 taatgatctg ctggcacgca ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa
    17461 aaagcagggc aagaatttcc acgcgtgttg tccattccac aacgagaaaa ccccgtcatt
    17521 caccgttaac ggtgagaaac agttttacca ctgctttgga tgtggcgcgc acggcaacgc
    17581 gatcgacttc ctgatgaact acgacaagct cgagttcgtc gaaacggtcg aagagctggc
    17641 agcaatgcac aatcttgaag tgccatttga agcaggcagc ggccccagcc agatcgagcg
    17701 ccatcagagg caaacgcttt atcagttgat ggacggtctg aatacgtttt accaacaatc
    17761 tttacaacaa cctgttgcca cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca
    17821 cgaggttatc gctcgctttg cgattggttt tgcgcccccc ggctgggaca acgtcctgaa
    17881 gcggtttggc ggcaatccag aaaatcgcca gtcattgatt gatgcgggga tgttggtcac
    17941 taacgatcag ggacgcagtt acgatcgttt ccgcgaacgg gtgatattcc ccattcgcga
    18001 taaacgcggt cgggtgattg gttttggcgg gcgcgtgctg ggcaacgata cccccaaata
    18061 cctgaactcg ccggaaacag acattttcca taaaggccgc cagctttacg gtctttatga
    18121 agcgcagcag gataacgctg aacccaatcg tctgcttgtg gtcgaaggct atatggacgt
    18181 ggtggcgctg gcgcaatacg gcattaatta cgccgttgcg tcgttaggta cgtcaaccac
    18241 cgccgatcac atacaactgt tgttccgcgc gaccaacaat gtcatttgct gttatgacgg
    18301 cgaccgtgca ggccgcgatg ccgcatggcg agcgctggaa acggcgctgc cttacatgac
    18361 agacggccgt cagctacgct ttatgttttt gccggatggc gaagaccctg acacgctggt
    18421 acgaaaagaa ggtaaagaag cgtttgaagc gcggatggag caggcgatgc cactctccgc
    18481 atttctgttt aacagtctga tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg
    18541 tttgagtacg ctggcactgc cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata
    18601 tcttcgtcag gaattaggca acaaattagg catacttgat gacagccagc ttgaacgatt
    18661 aatgccaaaa gcggcagaga gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac
    18721 catgcgtata cttatagggt tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc
    18781 gcttgagaat ctggatgaaa ataagctccc tggacttggc ttattcagag aactggtcaa
    18841 cacttgtctc tcccagccag gtctgacctc cgggcaactt ttagagcact atcgtggtac
    18901 aaataatgct gccacccttg aaaaactgtc gatgtgggac gatatagcag ataagaatat
    18961 tgctgagcaa accttcaccg actcactcaa ccatatgttt gattcgctgc ttgaactgcg
    19021 ccaggaagag ttaatcgctc gtgagcgcac gcatggttta agcaacgaag aacgcctgga
    19081 gctctggaca ttaaaccagg agctggcgaa aaagtgattt aacggcttaa gtgccgaaga
    19141 gcgatcggga agcccccgac agccgcactg agaggcagcg gcaaatatat aagtacgccc
    19201 tcgtaattat cgttggcggt aaacaaccgt tggatttcag cgttaacggc tgaaggacat
    19261 cgggtcaatc gcccaacacc aacctcatga aataagtgtg gataccgtct tatggagcaa
    19321 aacccgcagt cacagctgaa acttcttgtc acccgtggta aggagcaagg ctatctgacc
    19381 tatgccgagg tcaatgacca tctgccggaa gatatcgtcg attcagatca gatcgaagac
    19441 atcatccaaa tgatcaacga catgggcatt caggtgatgg aagaagcacc ggatgccgat
    19501 gatctgatgc tggctgaaaa caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg
    19561 ctttccagcg tggaatctga aatcgggcgc acgactgacc cggtacgcat gtacatgcgt
    19621 gaaatgggca ccgttgaact gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt
    19681 gaagacggga tcaaccaagt tcaatgctcc gttgctgaat atccggaagc gatcacctat
    19741 ctgctggaac agtacgatcg tgttgaagca gaagaagcgc gtctgtccga tctgatcacc
    19801 ggctttgttg acccgaacgc agaagaagat ctggcaccta ccgccactca cgtcggttct
    19861 gagctttccc aggaagatct ggacgatgac gaagatgaag acgaagaaga tggcgatgac
    19921 gacagcgccg atgatgacaa cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa
    19981 ctacgcgctc agtacgttgt aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct
    20041 gccgctcagg aagagatcct gaaactgtct gaagtattca aacagttccg cctggtgccg
    20101 aagcagtttg actacctggt caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa
    20161 gaacgtctga tcatgaagct ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt
    20221 accctgttta ccggcaacga aaccagcgat acctggttca acgcggcaat tgcgatgaac
    20281 aagccgtggt cggaaaaact gcacgatgtc tctgaagaag tgcatcgcgc cctgcaaaaa
    20341 ctgcagcaga ttgaagaaga aaccggcctg accatcgagc aggttaaaga tatcaaccgt
    20401 cgtatgtcca tcggtgaagc gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac
    20461 ttacgtctgg ttatttctat cgccaagaaa tacaccaacc gtggcttgca gttccttgac
    20521 ctgattcagg aaggtaacat cggcctgatg aaagcggttg ataaattcga ataccgccgt
    20581 ggttacaagt tctccaccta cgcaacctgg tggatccgtc aggcgatcac ccgttctatc
    20641 gcggatcagg cgcgcaccat ccgtattccg gtgcatatga ttgagactat caacaaactc
    20701 aaccgtattt ctcgccagat gctgcaagag atgggccgtg aaccgacgcc ggaagaactg
    20761 gctgaacgta tgctgatgcc ggaagacaag atccgcaaag tgctgaagat cgccaaagag
    20821 ccaatctcca tggaaacgcc gatcggtgat gatgaagatt cgcatctggg ggatttcatc
    20881 gaggatacca ccctcgagct gccgctggat tctgcgacca ccgaaagcct gcgtgcggca
    20941 acgcacgacg tgctggctgg cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc
    21001 ggtatcgata tgaacaccga ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc
    21061 cgcgaacgta tccgtcagat cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt
    21121 tctgaagtgt tgcgtagctt cctggacgat taatcggtag gccggatcag gcgttacgcc
    21181 gcacccggca ctagtctctc tgcacaaacg ccaccttt
//
LOCUS       DAJTYF010000066        21001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTYF010000066 DAJTYF010000000
VERSION     DAJTYF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.066"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..1601)
                     /gene="glyS"
                     /locus_tag="OO590_002493"
     CDS             complement(<1..1601)
                     /gene="glyS"
                     /locus_tag="OO590_002493"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6637819.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRA"
     gene            complement(1611..2522)
                     /gene="glyQ"
                     /locus_tag="OO590_002494"
     CDS             complement(1611..2522)
                     /gene="glyQ"
                     /locus_tag="OO590_002494"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6637820.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(2617..2916)
                     /locus_tag="OO590_002495"
     CDS             complement(2617..2916)
                     /locus_tag="OO590_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6637821.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3091..4086
                     /gene="wecH"
                     /locus_tag="OO590_002496"
     CDS             3091..4086
                     /gene="wecH"
                     /locus_tag="OO590_002496"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6637822.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4128..4565)
                     /locus_tag="OO590_002497"
     CDS             complement(4128..4565)
                     /locus_tag="OO590_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6637823.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(4611..4952)
                     /locus_tag="OO590_002498"
     CDS             complement(4611..4952)
                     /locus_tag="OO590_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637824.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5222..6574)
                     /gene="xylB"
                     /locus_tag="OO590_002499"
     CDS             complement(5222..6574)
                     /gene="xylB"
                     /locus_tag="OO590_002499"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6637825.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(6646..7968)
                     /gene="xylA"
                     /locus_tag="OO590_002500"
                     /pseudo
     CDS             complement(6646..7968)
                     /gene="xylA"
                     /locus_tag="OO590_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8334..9326
                     /gene="xylF"
                     /locus_tag="OO590_002501"
     CDS             8334..9326
                     /gene="xylF"
                     /locus_tag="OO590_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6637826.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEREL"
     gene            9404..10945
                     /gene="xylG"
                     /locus_tag="OO590_002502"
     CDS             9404..10945
                     /gene="xylG"
                     /locus_tag="OO590_002502"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6637827.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            10923..12104
                     /gene="xylH"
                     /locus_tag="OO590_002503"
     CDS             10923..12104
                     /gene="xylH"
                     /locus_tag="OO590_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR6637828.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12182..12712
                     /locus_tag="OO590_002504"
     CDS             12182..12712
                     /locus_tag="OO590_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6637829.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            12824..13360
                     /locus_tag="OO590_002505"
     CDS             12824..13360
                     /locus_tag="OO590_002505"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6637830.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGTRQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13468..14292)
                     /locus_tag="OO590_002506"
     CDS             complement(13468..14292)
                     /locus_tag="OO590_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6637831.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            14612..16642
                     /gene="malS"
                     /locus_tag="OO590_002507"
                     /pseudo
     CDS             14612..16642
                     /gene="malS"
                     /locus_tag="OO590_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            16820..18073
                     /gene="avtA"
                     /locus_tag="OO590_002508"
     CDS             16820..18073
                     /gene="avtA"
                     /locus_tag="OO590_002508"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6637832.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGETELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18224..18697)
                     /locus_tag="OO590_002509"
     CDS             complement(18224..18697)
                     /locus_tag="OO590_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6637833.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(18799..19647)
                     /gene="yiaJ"
                     /locus_tag="OO590_002510"
     CDS             complement(18799..19647)
                     /gene="yiaJ"
                     /locus_tag="OO590_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6637834.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            19848..20846
                     /gene="yiaK"
                     /locus_tag="OO590_002511"
     CDS             19848..20846
                     /gene="yiaK"
                     /locus_tag="OO590_002511"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6637835.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            20858..>21001
                     /locus_tag="OO590_002512"
     CDS             20858..>21001
                     /locus_tag="OO590_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6637836.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5289 a         5338 c         5158 g         5216 t
ORIGIN      
        1 gcgcggaagc gaccgagcat aaagtcgata acatcatcaa ctacgttggc attagtcagc
       61 ttatcgccat acagacgcac cgcttcttcg gtcagcgttt gcagatcaag gttgaggttc
      121 ttctcaacga taattcgcag cacgccaagt gcggcacgac gcagcgcaaa cgggtctttg
      181 tcgcctttcg gatgctgacc gataccgaag atacccgcca gagtatccat cttatcagca
      241 atcgccagcg cacaagctac cgggttggac ggcaggtcat caccggcaaa gcgcggctga
      301 tactgctcat tcagcgcgac ggctacgtct tccgcttcgc catcgtgacg cgcgtagtgc
      361 atccccataa cgccttgggt gtcggtgaac tcgaagacca tgttggtcat caggtcacac
      421 ttagacagca ggcccgcacg ggttgcgtgg ttaacgtcag cgccaatctg ttcggcgatc
      481 cagccagcca gcgcctggat gcggtcagtc ttgtcacgca gcgtacccag ctgttgctgg
      541 aacagcacgg tttgcaggcg cggcaggtta tcttccagac gttttttgcg gtcggtgttg
      601 aagaagaact cggcatccgc cagacgtgga cgaacgactt tctcgttacc agagataatt
      661 tgctgcggat ctttcgattc gatgttggca acgaagataa agttcggcag cagtttgccg
      721 tcgttcgcat acaccgggaa gtacttctgg tcacctttca tggtgtaaac cagggcttca
      781 gccggcaccg cgaggaattt ctcttcgaat tttgcggtca gaacgaccgg ccactccacc
      841 agcgaagcca cttcttccag caggctttcg cttaagtcag cgttaccgcc aatcttacgc
      901 gctgcttctt cggcatcggc tttaatcttc gctttacgtt cttcgtaatc ggcgatgact
      961 ttcccgcgct cacgcagaat ttccggatac tgatcggcgt tgtcgatggt gaattccggt
     1021 tcgcccataa agcggtggcc gcgaatcacg cgatcggact gaatgcccag aatggttgcc
     1081 ggaatgactt tgtcgcccag cagcagggtc acggtgtgca ccggacgcac gaagtgcacg
     1141 tcgcttgcac cccagcgcat cagtttcggg atcggcagtt tcgccagcga agtcgcaacc
     1201 atattcggca gcagtgcttc ggtgctttcg cccttcacat gggcgcgata cagcagccat
     1261 tcgcctttat cggtagtcag acgctcagcc tggtcaacgg taataccaca accacgcgcc
     1321 caaccttctg ccgctttgct cggttttcct tcagcgtcga acgcctgggc aatcgccggg
     1381 ccgcgttttt cgatttcacg atctggttgt gcttccgcca ggttagctac tttcagcgcc
     1441 agacgacgcg gagcagcaaa ccattgaacg gtgccgtgtg cgaggcctgc gttatccagc
     1501 tccgcagtaa agttcgcagc aaaggactct gccaggctgc gcagtgcttt tggtggcagc
     1561 tcttcagtgc cgatttccac cagaaaagtt ttctcagaca tagccgcctc ttacttatct
     1621 ttgttgcaca tcgggaagcc gagggcttca cgggaagcgt aatatgcttc tgccactgct
     1681 ttggtcaggg tgcgaatgcg cagaatgtag cgctgacgct cggtgacgga gatggcttta
     1741 cgcgcatcca gcaggttgaa gctgtgggcg gctttcagaa tacgctcgta ggctggcagc
     1801 ggcagcggat tttccagcgc cagcagctgc tgcgcttctt tctcgtactg ctcaaagcag
     1861 gtgaacagga agtccacatc cgcgtattcg aagttgtaag tggactgctc cacttcgttc
     1921 tgatggaaca cgtcgccgta ggtggtttta cccagcgggc cgtcgctcca gactaggtcg
     1981 taaacgctgt ctacgccctg aatgtacatg gccagacgtt ccagaccgta ggtgatctcg
     2041 ccggtaaccg gtttacactc cagaccacca acctgctgga agtaagtgaa ctgcgtcact
     2101 tccatgccgt ttagccacac ttcccagccc agtccccagg cccccagagt cgggttttcc
     2161 cagttatctt ccacgaaacg gatgtcgtga atagtcgggt ccatgcccag ctctttcaga
     2221 gaaccgaggt acagctcctg aatattgtcc ggcgatggct taatgaccac ctggaactga
     2281 tagtagtgct gtaaacggtt ggggttttcg ccgtagcgac catcggtcgg gcgacgagaa
     2341 ggctgaacat aagcagccgc catcggttct ggccccagcg cgcgcagaca ggtcattggg
     2401 tgagaggttc ccgcgccgac ttccatgtcc aatggttgaa caatggtgca gccctggcga
     2461 gcccagtaat cctgtaaggt caggatcaag ccctggaagg tcctggtatc aaacttttgc
     2521 atattatttc gtgctggata cgtgtggatt taaaggaagg gatcagtata cccgctggat
     2581 ggaagatata cagtacgaaa cgggaaaaag cagggcttaa cgcatggaaa gatgcaaaaa
     2641 atggccatct gcgtcaaaag aacagacaaa ctgctcttta cggaaggtat aaccgcgcat
     2701 ttcatagctt ccctggaact gttcgaaggc ggtgacgtcg attttttgct cgccggtgtt
     2761 ataacgtttt gccgcctgat ccttgcataa cgcttccata ttcaacgaac gcagaggatc
     2821 aacctttacc cgttgtgctt tctgcacggg tgacggtgta gaacaaccca ctagcaccat
     2881 aagcgccatt gccggaaaga aagcgttcat catcattaaa ttaccgtctg gtttgcaggt
     2941 gagtcttatt atttatatcg gtatagtggc tgatatctta cgaatttcct taagcctcta
     3001 taggctgtag tgataaaact cagattattc cagtgcgcca accaaatgat ttatcggtca
     3061 gacttttttt aaccagatac agagacacag atgcagccca aaatttactg gattgataac
     3121 ctgcgaggga tagcgtgttt aatggtggtg atgattcaca ccactacctg gtatgtgacc
     3181 aatgctcata gtgttagccc cgtcacatgg gatatcgcca atgttctgaa ttctgcctct
     3241 cgtgtcagcg tgccgctatt tttcatgatt tccggctatc tcttttttgg cgaacgcagc
     3301 gcccagccgc gccatttctt gcgtatcggc ttatgtctga ttttttatag cgcaatcgca
     3361 ctgctctaca ttgcgctgtt tacctccatc aatatggagt tagcgctgaa aaacctgctg
     3421 caaaagccag tgttttacca cttgtggttt ttcttcgcga ttgcggtgat ttatctggtt
     3481 tcaccgctga ttcaggtgaa gaacgtcggc ggaaaaatgt tgctggtgct aatggtggtg
     3541 attggcatta tcgctaaccc aaacacagtg ccgcagaaaa ttgacggttt tgaatggctg
     3601 ccaattaact tatatatcaa tggcgatact ttttactaca ttctgtatgg catgttgggc
     3661 cgcgctatag ggatgatgga cacacagcat aaagcactgt cgtgggtgag cgccgcgctg
     3721 tttgcgacgg gggtttttat tatctctcgc gggacattat atgaattgca gtggcgcgga
     3781 aattttgccg atacctggta tctttactgt gggccgatgg tttttatctg cgcaatcgcg
     3841 ctattgactc tggttaaaaa cacgctggat acgcgtacca ttcgcggact tggcttaatc
     3901 tcccgccatt cattgggtat atacggattc cacgccttga ttatccatgc gctgcgcacc
     3961 cggggaattg agcttaaaaa ttggccaata ctggatatta tttggatctt ttgcgcgacg
     4021 ttggcagcga gtttgttact ttctatgctg gtacaacgaa tcgacagaaa cagattagtg
     4081 agttaagtaa aagcccgttc acattggact gaccgggctt acgtgaatta ttcgctgtat
     4141 tcttcctggg taacctgtgt ttcttttggc gggtttattc cggcatcacg aatattcttc
     4201 tgcaccgcta ctgcaccaaa caagcttaag aagaaagcca gtccataaaa acctttttca
     4261 ctgagtaata atgtcgcatt ccacagacct accatcagta gagcaacaga gataataaag
     4321 acagtcaggc aggtcatata ataaatggaa gtggtcggta tgccttcata cttgtcccga
     4381 acggtctttt gataagacgc agcagaaaac agtcctaaca ccagtacggc aaaataatac
     4441 cctttttcat ttaactgcat ctctgcattc catagcccta acaggtaggt aacgatacca
     4501 ccaacgagag ctatccatga cacaatacta aaggccggtg aataggttga tattttgttg
     4561 tccatcacag tattcctttc attcctgaat gtgtaagagc tttatgttgc tcataccgag
     4621 cgaaacaaac gcatttgacc aaacaagcag acaaaaaagg cgatggggta gatcaccatt
     4681 tcataagcat tgataggtgc atttaaaaca ccgataaaca agagaccgat agtgattaac
     4741 gcaactaact ggcagatatg ggtaaaaaaa ttatccagtt gcccgagttt ttctgcgcga
     4801 agatatgcat agttaccaaa agttgctgtc attaacacgc caagaaaata gccttttcca
     4861 cttagcaatg ggcatgagat ccatagccca accagataaa ccagcgcccc gacaacaaat
     4921 aatagttttg ccacagtttt tgaggttttc ataacgatct ccatatctac cagcgataca
     4981 ttacgagtaa ccaacgaaag acaaaactga aaaatgccat taacaaatga ttttcagaat
     5041 aaattcatac taaatattaa ttaattagtg agatatatag atgtgaatta tcccccaccc
     5101 ggtcaggcag gggataacgt ttacgccatt aatggcagaa gttgctgata gaggcgacgg
     5161 aacgtttcac gtcgtggctg ataagcggca taacgctgcg catctggtag atgcgactgt
     5221 tctaacggta gctgcggcaa caactcaatg agcgatttct ctggattcgc cgcaatctgc
     5281 gccagccttg ctgcgcccag tgctggcccc acatcccccc cgtacggtaa tcgagctgct
     5341 gaccgctgat atccgccagc atctgacgcc agtactcact acgcgccccg ccaccaatca
     5401 acgtaacact ttgcggttta atgccgcagg catgcacgac atccatgcca tctgccagcg
     5461 cataacccac gccttccagc actgctcgcg ccagttcatt ggggccatgt tgatgagtca
     5521 aaccaaagaa aacccccttc gcctggggat tattgtgtgg tgtacgctcg ccggaaagat
     5581 aaggcagaaa ccaaactggc tcggcacttt catcagcctg ttgagctgca gcgattaaag
     5641 ctgggacatt gcacaggccg gttaatttcg cggcccaatc cagacacgat gctgcactca
     5701 gcattacaga cattaaatgc caacgctgcg gtagcgcatg gcaaaagctg tgtacagcgc
     5761 tttctggctt gcttaagaac ccttcgctga cagcaaaata gacccccgac gtccccagcg
     5821 ataacattgc ctgattagca tcaaccattc ccacaccaac tgcaccagct gcattgtcgc
     5881 caccgcctgc gacaactggc accgtcgcca taccccacgc tttcgcaact tcaggtaaca
     5941 aagcaccagt aatttcgctg ccttcgtata atgcgggcat ctggtcacga gataagtcgc
     6001 aagcctgcag catgacgtca ctccagtcac gctttgcgac atccagccac atggtgccag
     6061 ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg cagacgcaag taatctttcg
     6121 gtaataatac tttgtcgatt tgacggaata tctccggctc atgccgctga acccatagca
     6181 atttaggcgc agtaaatccg ggcatcatca ggttgccggt aatcactcgt gattgcggaa
     6241 ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc gtcgttccac aaaatggcag
     6301 ggcgcaatac ccgttgttga gcatccagta aggttgctcc atgcatctgg ccggcaatac
     6361 ccaatgcttt aacgtcctgc agagaatgct gatcgcccag agctttcatt gcgcgatcag
     6421 ttgcctgcca ccactgttcc gggtcttgtt ccgaccaaag tggatgcggg cgcgaaacgg
     6481 tcagcttttc cgtttgcgaa gcaaccacct caccctgctc gttgagcaaa ataactttta
     6541 cgcccgaggt gccaagatct atcccgatat acatatcgat cgttccttaa aaaaatgccc
     6601 ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt agccgttatt tgtcgaacag
     6661 ataatgattt accagatttt ccagttgctc ctggcgacca ctctgatgca ccggagacaa
     6721 attatgttcc tgagcatatt tggctaaatc tgccagtgac atttggcctt tcaggatttg
     6781 ctggcccaat tcgctattcc agccggaata acgctgcgcg atgcgtttat ccagctcgcc
     6841 atcttcaatc atgcgcgctg caattttcag cgccagtgcc atcgtatcca tcgcgccgat
     6901 atgaccgtaa aacagatcat atttatcagt actttgacga cgtactttgg catcgaagtt
     6961 cagaccaccg gtggtgaaac cgcctgcttt gagaatttca tacatcacca gcgcattctc
     7021 ttccacactg ttcgggaact ggtcggtgtc ccagcccagt tgcgcatcgc cacggttggc
     7081 gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct atttcatgat ggaaagagtg
     7141 acctgccagc gtcgcgtggt tagcttcaat gttcagttta atctcttttt ccagaccaaa
     7201 ctgtttcagg aagccataga ccgtcgcggc atcgtaatca tattgatgtt tagtcggttc
     7261 ttgcggtttc ggttcgataa gcaacgtgcc ctagaagccg attttatgtt tatgctcaac
     7321 caccatctgc ataaagcggc ccagttgttc acgctcctga cgcaagtcgg tatttaacag
     7381 cgtttcgtaa ccttcacgac caccccacag gacatagttt tcaccgccca atttatgggt
     7441 tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg aagacttcag gatctgggtt
     7501 cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag ttagcggttc cccacagcag
     7561 cttcacgccg ctctcttctt gcttggctgc caggacatca accatttgcg caaaattatt
     7621 gatgtactct tttaacgacg cgccctcagg ggaaacatcc acatcgtgga agcaataaaa
     7681 tggcacatgt aacttgtgga aaaactcaaa tgcgacatct gctttacgct tcgccaacgc
     7741 cagtgcctca ccaggctgct gccacggacg attaaacgcc cccacaccaa acatgtccgc
     7801 cccgttccag cagaaggtgt gccagtagca ggcggcaaaa cgcaagtgct cttccatacg
     7861 cttacccaac accagttcgt cgggattgta gtgacggaat gctaacgggt ttgaggattt
     7921 tgagccttca taacgaacgc gatcgagctg gtcaaaatag gcttgcatat tgaactccat
     7981 aatcaggtaa tgccgcgggt gatggatgat gtcgtaatat tgggcactcc ctttcaattg
     8041 ctcaattatg ttatttcaca ctgctattga gataattcac aagtgtgcgc tcgctcgcaa
     8101 aataaaatgg gatgatgaaa ctgggtaatt cctcgaagat aaaaatgcaa taagtacaat
     8161 tgcgcaacaa aagtaagatc tcggtcataa atcaagaaat aaaccaaaaa tcgtaatcga
     8221 aagataaaaa tctgtaattg ttttcccctg tttagttgct aaaaattggt tacgtttatc
     8281 gcggtgattg ttacttatta aagctgtcct ctaactacag aaggccctac accatgaaaa
     8341 taaagaacat tctactcacc ctttgcacct cacttctgct taccaacgtt gcggcacacg
     8401 ccaaagaagt caaaataggt atggcgattg atgatctccg tcttgaacgc tggcaaaaag
     8461 atcgagatat ttttgtgaaa aaggcagaat ctctcggtgc gaaagtattt gtacagtctg
     8521 caaatggcaa tgaagaaaca caaatgtcgc agattgaaaa catgattaac cggggtgtcg
     8581 atgttcttgt cattattccg tataacggtc aggtattaag taacgttgta aaagaagcca
     8641 aacaagaagg tattaaagta ttagcttacg accgtatgat taacgatgcg gatatcgatt
     8701 tttatatttc tttcgataac gaaaaagtcg gtgaactgca ggcaaaagcc ttggtcgata
     8761 ttgttccgca aggtaattac ttcctgatgg gcggctcgcc ggtagataac aacgccaagc
     8821 tgttccgcgc cggacaaatg aaagtgttaa aaccttacgt tgattctgga aaaattaaag
     8881 tcgttggtga ccaatgggtt gatggctggt taccggaaaa cgcattgaaa attatggaaa
     8941 acgcgctaac cgccaataat aacaaaattg atgctgtagt tgcctcaaac gatgccaccg
     9001 caggtggggc aattcaggca ttaagcgcgc aaggtttatc agggaaagta gcaatttccg
     9061 gccaggatgc ggatctcgca ggtattaaac gtattgctgc cggtacgcaa actatgacgg
     9121 tgtataaacc tattacattg ctggcaaata ctgccgcaga aattgccgtt gaattgggca
     9181 atggtcagga gccaaaagca gatacctcac tgaataatgg cctgaaagat gtcccctccc
     9241 gcctcctgac accgatcgat gtgaataaaa acaacatcaa agatacggta attaaagacg
     9301 gattccacaa agagagagag ctgtaagcgt tacgccccgg cgcggagcgg gggcgtgatt
     9361 tctctccatg ccgcgtgaat gaattggctt aggtggagtc gttatgcctt atctacttga
     9421 aatgaagaac attaccaaaa ccttcggcag tgtgaaggcg attgataacg tcagcttgcg
     9481 gttgaatgct ggcgaaatcg tctcactctg tggggaaaat gggtctggta aatcaacgct
     9541 gatgaaagtg ctgtgtggta tttatcccca tggctcctac gaaggcgaaa ttatttttgc
     9601 gggagaagag attcaggcga gtcacatccg cgataccgaa cgcaaaggta tcgccatcat
     9661 tcaccaggaa ttggccctgg tgaaagaatt gaccgtgctg gaaaatatct tcctgggtaa
     9721 cgaaataacc cacaatggca ttatggatta tgacctgatg acgctacgct atcagaagct
     9781 gctcgcacag gtcagtttat ccatttcacc tgatacccgc gttggcgatt tagggcttgg
     9841 gcaacaacaa ctggttgaaa ttgccaaggc acttaataaa caggtgcgct tgttaattct
     9901 cgatgaaccg acagcctcat taactgagca ggaaacgtcg gttttactgg atattattcg
     9961 cgatctacaa cagcacggta tcgcctgtat ttatatttcg cacaaactca acgaagtcaa
    10021 agcgatttcc gatacgattt gcgttattcg cgacggacag cacattggta cgcgtgatgc
    10081 tgccggaatg agtgaagacg atattatcac catgatggtc gggcgagagt taaccgcgct
    10141 ttaccctaat gaaccacata ccaccggaga tgaaatatta cgtattgaac atctgacggc
    10201 atggcatccg gtcaatcgtc atattaaacg agttaatgat gtctcgtttt ccctgaaacg
    10261 tggcgaaata ctgggtattg ccggactcgt tggtgccgga cgtaccgaga ccattcagtg
    10321 cctgtttggc gtgtggcccg gacaatggga aggaaaaatt tatattgatg gcaaacaggt
    10381 agatattcgt aactgtcagc aagccatcgc ccaggggatt gcgatggtac ccgaagacag
    10441 aaagcgcgac ggcatcgttc cggtaatggc ggttggtaaa aatattaccc tcgccgcact
    10501 caataaattt accggtggca ttagccagct tgatgacgcg gcagagcaaa aatgtattct
    10561 ggaatcaatt cagcaactca aagttaaaac gtcgtccccc gaccttgcta ttggacgttt
    10621 gagcggcggc aatcagcaaa aagcgatcct cgcccgctgt ctgttactta acccgcgcat
    10681 cctcattctt gatgaaccta ccaggggtat tgatattggc gcgaaatatg agatctacaa
    10741 attaattaac caactcgtcc agcagggtat tgccgttatt gtcatctctt ccgaattacc
    10801 tgaagtgctc ggccttagcg atcgtgtact ggtgatgcat gaagggaaac taaaagccaa
    10861 cctgataaat cataacctga ctcaggagca ggtgatggaa gccgcattga ggagcgaaca
    10921 tcatgtcgaa aagcaatccg tctgaagtga aattggccgt accgacatcc ggtggctttt
    10981 ctgggctgaa atcactgaat ttgcaggtct tcgtgatgat tgcagctatc atcgcaatca
    11041 tgctgttctt tacctggacc accgatggtg cctacttaag cgcccgtaac gtctccaacc
    11101 tgttacgcca gaccgcgatt accggcatcc tcgcggtagg aatggtgttc gtcataattt
    11161 ctgctgaaat cgacctttcc gtcggctcaa tgatggggct attaggtggc gtcgcggcga
    11221 tttgtgacgt ctggttaggc tggcctttgc cacttaccat cattgtgacg ttggttctgg
    11281 gactgcttct cggtgcctgg aacggatggt gggtcgcgta ccgcaaagtc ccttcattta
    11341 ttgtcaccct cgcgggcatg ttggcatttc gcggcatact cattggcatc accaacggca
    11401 cgactgtatc ccccaccagc gccgcgatgt cacaaattgg gcaaagctat ctccccgcca
    11461 gtaccggctt catcattggc gcgcttggct taatggcttt tgttggttgg caatggcgcg
    11521 gaagaatgcg ccgtcaggct ttgggtttac agtctccggc ctctaccgca gtagtcggtc
    11581 gccaagcttt aaccgctatc atcgtattag gcgcaatctg gctgttgaat gattaccgtg
    11641 gcgttcccac tcctgttctg ctgctgacgt tgctgttact cggcggaatg tttatggcaa
    11701 cgcggacggc atttggacga cgcatttatg ccatcggcgg caatctggaa gcagcacgtc
    11761 tctccgggat taacgttgaa cgcaccaaac ttgccgtgtt cgcgattaac ggattaatgg
    11821 tagccatcgc cggattaatc cttagttcta gacttggcgc tggttcacct tctgcgggaa
    11881 atatcgccga actggacgca attgcagcat gcgtgattgg cggcaccagc ctggctggcg
    11941 gtgtgggaag cgttgccgga gcagtaatgg gggcatttat catggcttca ctggataacg
    12001 gcatgagtat gatggatgta ccgaccttct ggcagtatat cgttaaaggt gcgattctgt
    12061 tgctggcagt atggatggac tccgcaacca aacgccgttc ttgattttga taaaaatttt
    12121 ctcaaagccg gttacgtatt accggttttg agtttttgca tgattcagca ggaaaagaac
    12181 catgtttact aaacgtcacc gcatcacatt actgttcaat gccaataaag cctatgaccg
    12241 gcaggtagta gaaggcgtag gggaatattt acaggcatca caatcagaat gggatatttt
    12301 cattgaagaa gatttccgcg cccgcattga taaaatcaag gactggttag gagatgtcgt
    12361 cattgccgac ttcgacgaca aacagatcga gcaagcgctg gctgatgtcg acgtccccat
    12421 tgttggggtt ggcggctcgt atcaccttgc agaaagttac ccacccgttc attacattgc
    12481 caccgataac tatgcgctgg ttgaaagcgc atttttgcat ttaaaagaga aaggcgttaa
    12541 ccgctttgct ttttatggtc ttccggaatc aagcggcaaa cgttgggcca ctgaacgcga
    12601 atatgcattt cgtcagcttg tcgccgaaga aaagtatcgc ggagtggttt atcaggggtt
    12661 agaaaccgcg ccagagaact ggcaacacgc gcaaaatcgg ctggcagact gactacaaac
    12721 gctgccaccg caaaccggga ttattgccgt tactgacgcc cgagcgcggc atattctgca
    12781 agtatgtgaa catctacata ttcccgtacc ggaaaaatta tgcgtgattg gcatcgataa
    12841 cgaagaactg acccgctatc tgtcgcgtgt cgccctttct tcggtcgctc agggcacgcg
    12901 gcaaatgggc tatcaggcgg caaaactgtt gcatcgatta ttagataaag aagaaatgcc
    12961 gctacagcga attttggtcc caccagttcg cgtcattgaa cggcgctcaa cagattatcg
    13021 ctcgctgacc gatcccgccg ttattcaggc catgcattac attcgtaatc acgcctgtaa
    13081 agggattaaa gtggatcagg tactggatgc ggtcgggatc tcgcgctcca atcttgagaa
    13141 gcgttttaaa gaagaggtgg gtgaaaccat ccatgcaatg attcatgccg agaagctgga
    13201 gaaagcgcgc agtctgctga tttcaaccac cttgtcgatc aatgagatat cgcaaatgtg
    13261 cggttatcca tcgctgcaat atttctactc tgtttttaaa aaagcatatg acacaacgcc
    13321 aaaagagtat cgcgatgtaa atagcgaggt catgttgtag cgatgatgag aattgtcggc
    13381 gtcacatcag ggaactcagc acagccacct gatgcgagca aaagccttat ccgaccgaca
    13441 aatcagataa ggctcgagga aatgaaatta catatgcgcc gcgattaacc gttggttatc
    13501 ctggtacatt gcgaacaggt agttgttata actcttccct ttggtcgagt agcccttcag
    13561 cttgtgaatc atcgctgtgg cagtcacttc ctgatccgct ttacgcagct gtgcacgcga
    13621 tttacggaac gaagagtaag ccgggtgcgt attcaggtta gtgacatagg cgctcaccga
    13681 ttctttgaca gaactaaact gtgagtaccc tttcacttta cccggcgcat tggtacaacg
    13741 tcctttcatg catttcatgc cgaacagatt gttattgttg cgcgccagct tcgacgttcc
    13801 ccaaccgctt tctgctgcag ccatcgtcgc caccatactg gtagggataa tgtctacgcg
    13861 ttcaagcaag gtattccacg ggatttttcg cgtattacca gaccacttca ccttgtagcg
    13921 tttggcgatg tctttcagac gcgcacgctc agcaggtgac cattggccct gatactgttt
    13981 tgaaatcagc cagttacgtt ctgcagtaat ggctgcattt tggctggtaa tgtaaggcat
    14041 tacggtccgg agaaacgcct ttttccttgg tgttccggaa gggtattttc gcaaatcagg
    14101 aagtgaacta ctctttgcac tattgcgaga atactcttgt ttactgctta cctgtttatt
    14161 actagcttta gttaagtggg acttttgact cgctgttgtt gtgtgcgtct tcgctaacac
    14221 ctcactcgaa aacaccagag tgagtaacat aagaatcatc gccccatatc gtcgtatggg
    14281 agtcaaaatc atcaggtctc ctggccggat ttaatcattc caacacctta tatttttcac
    14341 aaatttgaga gttgaatctc aaatcatatc aaaaatagct gtcaagagca ccccaaggaa
    14401 tagtccaaat ctgaaactat gtcacgagtt aacgattcag attggcgcta aatcgcagaa
    14461 aatgtggggg ttatcgcaaa attcagccgt tttttgcgcg cgatcgctca cccttgcttc
    14521 tcatcctgtg gacttaccgc tcagggatga gttttgtctg gcttatcgct ggcaaactgt
    14581 ctgaaatcgc agcaataagg actcatccac catgaaactc gccgcctgtt ttctgacact
    14641 ccttcctggc ttcgccgttg ccgccagctg gacttctccg gggttccctg cctttagcga
    14701 acagggaacg ggaacatttg tcagccacgc gcagttgccc aaaggtacgc gtccactcac
    14761 gctaaatttt gaccaacagt gctggcagcc tgcagatgcg ataaaactca atcagatgct
    14821 ttccctgcaa ccttgtagca acacgccgcc tcaatggcga ttgttcaggg acggcaaata
    14881 tacgctgcaa atagacaccc gctccggtac gccaacattg atgatttcca tccagaacgc
    14941 cgccgaaccg gtagcaaacc tggtccgtga atgcccgaaa tgggatggat taccgctcac
    15001 gctggatgtc agcgccactt tcccggaagg agccgccgta cgggattatt acagccaaca
    15061 aattgcgata gtgaagaacg gtcaaataac gttacaaccc gctgctacca gcaacggttt
    15121 actcctgctg gaacgggcag aaactgacac atccgcccct ttcgactggc ataacgccac
    15181 ggtttacttt gtgctgacag atcgtttcga aaacggcgat cccagtaatg accagagtta
    15241 cggacgtcat aaagacggta tggcggaaat tggcactttt cacggcggcg atttacgcgg
    15301 cctgaccaac aaactggatt acctccagca gttgggcgtt aatgctttat ggataagcgc
    15361 cccatttgag caaattcacg gctgggtcgg cggcggtaca aaaggcgatt tcccgcatta
    15421 tgcctaccac ggttattaca cacaggactg gacgaatctt gatgccaata tgggcaacga
    15481 agccgatcta cggacgctgg ttgatagcgc acatcagcgc ggtattcgta ttctctttga
    15541 tgtcgtgatg aaccacacca gctatgccac gctggcggat atgcaggagt atcagtttgg
    15601 cgcgttatat ctttctggtg acgaagtgaa aaaaacgctg ggtgaacgct ggagcgactg
    15661 gaaacctgcc gccgggcaaa cctggcatag ctttaacgat tacattaatt tcagcgacaa
    15721 aacaggctgg gataaatggt ggggaaaaaa ctggatcaga acggatatcg gcgattacga
    15781 caatcctgga ttcgacgatc tcactatgtc gctggccttt ttgccggata tcaaaaccga
    15841 atcaactacc gcttctggtc tgccggtgtt ctataaaaac aaaacggata ctcacgctaa
    15901 agtcatcgaa ggctttacac ctcgcgatta cttaacccac tggttaagtc agtgggtccg
    15961 cgactatggg attgatggtt ttcgggtcga taccgccaaa catgttgagt tgcccgcctg
    16021 gcagcaactg aaaaccgaag ccagctccgc gcttcgcgaa tggaaaaaaa ctaaccccga
    16081 caaagcatta gatgacaaac ctttctggat gaccggtgaa gcctagggcc acggcgtgat
    16141 gcaaagtgac tactatcgcc acggcttcga tgcgatgatc aatttcgatt atcaggagca
    16201 ggcggcgaaa gcagtcgatt gtctggcgca gatggatacg acctggcagc aaatggcgga
    16261 gaaattgcag ggtttcaacg tgttgagcta cctctcgtcg catgataccc gtctgttccg
    16321 tgaagggggc gacaaagcag cagagttatt actattagcg ccaggcgcgg tacaaatctt
    16381 ttatggcgat gaatcctcgc gtccgttcgg tcctacaggt tctgatccgc tgcaaggtac
    16441 acgttcggat atgaactggc aggatgttag cggtaaatct gccgccaacg tcgcgcactg
    16501 gcagaaaatc agccagttcc gcgcccgcca tcccgcaatt ggcgcgggca aacaaacgac
    16561 actttcgctg aagcagggct acggctttgt tcgtgagcat ggcgacgata aagtgctggt
    16621 catctgggct gggcaacagt gacttttccg gcttcccgtt cgtcagtacc tcgggaagcc
    16681 gccaaccagg ataaaatgtc agccctaatc agcgttgcgg gataaagcac cgctcactct
    16741 tcaacagacc gatttgcacc ccagcaaatg tagcgttatt gttaccttcc ttgctacaga
    16801 gttcgacaga tatcccgcta tgacattctc cctttttggt gacaaattta cccgccactc
    16861 cggcattacg ctgctgatgg aagatctgaa cgacggttta cgcacgcctg gcgcgattat
    16921 gctcggcggc ggtaatccgg cgcagatccc ggaaatgcag gactacttcc agacgctact
    16981 gaccgacatg ctggaaagtg gcaaagcgac tgatgcactg tgtaactacg acggtccaca
    17041 gggggaaacg gagctactca cactgcttgc cggaatgctg cgcgagaagt tgggttggga
    17101 tatcgaacca cagaatattg cactaacaaa cggcagccag agcgcgtttt tctacttatt
    17161 taacctgttt gccggacgcc gtgccgatgg tcgggtcaaa aaagtgctgt tcccgctggc
    17221 accggaatac attggctacg ctgacgccgg actggaagaa gatctgtttg tctctgcgcg
    17281 tccgaatatt gaactgctgc cggaaggcca gtttaaatac cacgtcgatt ttgagcatct
    17341 gcatattggc gaagaaaccg ggatgatttg cgtctcccgg ccgacgaatc caacaggcaa
    17401 tgtgattact gacgaagagt tgctgaagct tgacgcgctg gcgaatcaac acggcattcc
    17461 gctggtgatt gataacgctt atggcgtccc gttcccgggt atcatcttca gtgaagcgcg
    17521 cccgctatgg aatccgaata tcgtactgtg catgagtctt tccaagctgg ggctgcctgg
    17581 ctcccgctgc ggcattatca tcgccaatga aaaaatcatc actgccatca ccaatatgaa
    17641 cggcattatc agcctggcac ctggcggtat tggtccggcg atgatgtgtg aaatgattaa
    17701 gcgtaacgat ctgctgcgcc tgtctgaaac agtcatcaaa ccgttttact accagcgtgt
    17761 tcaggaaact atcgccatca ttcgccgcta tttaccggaa aatcgctgcc tgattcataa
    17821 accggaagga gccattttcc tctggctatg gtttaaggat ttgcccatta cgaccgagca
    17881 gctctatcag cgcctgaaag cacgcggcgt gctgatggtg ccggggcaca acttcttccc
    17941 gggactggat aaaccgtggc cgcatacgca tcaatgtatg cgcatgaact acgtaccaga
    18001 gccggagaaa attgaggcgg gggtgaagat tctggcggaa gagatagaaa gagcttgggc
    18061 agaaagtcac taaaaattgc ctgatgcgct acgcttatca ggcctacatg atttctgcaa
    18121 tatattgaat ttggaagaat ttgtaggccg gataaggcgt ttacgccgca tctggcataa
    18181 acaaagcgca ctattccggc gttgagaaac gccggaaaac gtttcaaaca ggctgctgct
    18241 gtagccgatg ccgctgcact ttctcgacat ccatgcactg caacgccttt gtcgggcagg
    18301 cttcaacaca ggccggcccc gtctcccgat gccagcacag atcgcactta atcgccctcg
    18361 cctttttgcg cgaagaaacg acctccatcg caccaaacgg gcaagccagc atacagcttt
    18421 tacagccaat gcaacgtgtt tgttcaacga aaatatgccc atgttcgcgg cttatcgcgt
    18481 caacagggca gacattcgcg cacggtgcat cttcacactg atgacaggct actggcgtgg
    18541 tccagctgtg gtctttaatg acacgaatac gggaaataaa ctcgtctggt gacaacgcag
    18601 cgcaatcctg attctcctga tgcgacactg cgcaagccac ttcacaggta cggcaaccga
    18661 tacatttcgt cgcatccgca ataataaacc ggttcatgat cattcctttg cactaaaaca
    18721 gcagagtgcc agagagcgcg tccctactgc tttgatccaa cgaaggaaat gttaaagttt
    18781 tggcacttgt ccaaagcgtt atgtgagcgc gcccaggtca tccctgacag taaatcccag
    18841 ttcattagaa atagcctgcg cggtttcacg cagtggtttc aggagatttt tctctcccac
    18901 ctgtttcaga cgtgatgtcg aaagcgaaat cgacacggcg tacggcaccc gcccatgaat
    18961 atcaaacacc ggaacagcaa tacaggagac gccgagttcg ttttcttctc tgtccatcgc
    19021 cgctccgctt tcacgaatgt gcgccagttc gtcgaacatc gcgggcagct cggtaatggt
    19081 attgtgggtt aacggctgga tctcatgctg atggttttcc cagtatgact tcacgtagtc
    19141 cgggtgacca aacgccatgt agatcttgcc cattgcggaa cagtagagcg gcatatgctg
    19201 gccaatatag gcacgggttc gcagcatccc ggttgtgggt tccagcttat aaatcaaaat
    19261 agcgtgatcg tcttcgcggc tggagaagtt aatggtttca ccagtggcga tgttcagtgc
    19321 ctcaagatgc ggagcggcga tatgaatgat attcagcgaa gacagcgcct tctgcccgac
    19381 ggcaataaat ttggtagtca ggcggtaact ccctgcggcg ggcgcggtgg tcacatagcc
    19441 gcaggactgt aatccctgca ataagcgatg gacggtactc ttatttaaac cagccagctc
    19501 cgaaagatgc gccaacggac aaccgtttgg atagttgctc aaaatctcaa tcagcatcaa
    19561 cccgcgaaac agactctggc ttccggctgg acgctctttt tcctgcgcca tctcgttctc
    19621 tttttttccc atcacttctt tccccatttt gtcgcgtcct gatggtagcg caaagtgtgc
    19681 cgtagttcac gatctcgaca gataatttat aaccacttga tttttatgtc ttttgaaatt
    19741 catcaatcag attgctttgt taaaaagtga tcgatatatt tgaaatctag tttcgcatat
    19801 tgaaatttta agacaaaaaa gcgatcaaaa aaacaaggaa gcctaggatg aaagtgacat
    19861 ttgagcagtt aaaagcagcc tttaatcggg tcttaatttc acgcggcgtt gacagcgaaa
    19921 cggctgacgc ctgtgcagag atgttcgccc gcaccaccga atccggcgtt tattctcacg
    19981 gcgttaatcg tttccctcgt ttcattcaac aactggaaaa cggcgatatc attcctgatg
    20041 cccaacccaa acgtataacc agcctcggcg caattgaaca gtgggatgcc cagcgttcga
    20101 tcggcaacct gacggcgaaa aagatgatgg atcgcgccat tgaactggct gccgatcacg
    20161 gtattggtct ggtggcactt cgtaatgcca accactggat gcgcggcggc agctacggct
    20221 ggcaggcggc ggaaaaaggc tatattggca tttgctggac caactccatc gccgtaatgc
    20281 cgccgtgggg cgcaaaagag tgtcgcatcg gcactaaccc gctgatcgtc gccattcctt
    20341 ccacgccgat caccatggtc gatatgtcga tgtcgatgtt ctcttacggc atgttagaag
    20401 ttaaccgcct ggcaggccgt cagctcccgg tcgatggtgg ctttgatgat gagggcaatt
    20461 tgaccaaaga acctggcgtt atcgagaaga atcgccgcat tttgccgatg ggctactgga
    20521 aaggttctgg catgtcgatt gtgctggata tgatcgctac tctcctttcc gacggcgcat
    20581 cggttgccga agtcacccag gacaacagcg acgaatacgg catttcacaa atttttattg
    20641 ccattgaagt ggacaagctt atcgacggtc ccacccgcga tgccaagctg caacgtatca
    20701 tggattacgt tactaccgct gaacgcgctg acgaaaacca ggccatccgc ttacccggcc
    20761 acgaatttac taccctgctg gccgaaaacc gccgtaacgg catcactgtt gatgacagcg
    20821 tgtgggccaa aatccaggcg ttatgaggag ataagtcatg atttttggac atatcgcgca
    20881 gcccaatccg tgccgtttgc ccgcagccat tgaaaaggcg ctcgattttc tgcgcgctac
    20941 caacttcaac gtcctggagc cgggcgttgt cgaaatcgac ggcaagaata tttatgcgca
    21001 a
//
LOCUS       DAJTYF010000067        20700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTYF010000067 DAJTYF010000000
VERSION     DAJTYF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.067"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(85..4545)
                     /gene="mukB"
                     /locus_tag="OO590_002513"
     CDS             complement(85..4545)
                     /gene="mukB"
                     /locus_tag="OO590_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6637837.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4545..5249)
                     /gene="mukE"
                     /locus_tag="OO590_002514"
     CDS             complement(4545..5249)
                     /gene="mukE"
                     /locus_tag="OO590_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6637838.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5230..6552)
                     /gene="mukF"
                     /locus_tag="OO590_002515"
     CDS             complement(5230..6552)
                     /gene="mukF"
                     /locus_tag="OO590_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6637839.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6549..7334)
                     /gene="cmoM"
                     /locus_tag="OO590_002516"
     CDS             complement(6549..7334)
                     /gene="cmoM"
                     /locus_tag="OO590_002516"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6637840.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7470..8249
                     /gene="elyC"
                     /locus_tag="OO590_002517"
     CDS             7470..8249
                     /gene="elyC"
                     /locus_tag="OO590_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6637841.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8226..9119)
                     /locus_tag="OO590_002518"
     CDS             complement(8226..9119)
                     /locus_tag="OO590_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6637842.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9273..10019)
                     /gene="kdsB"
                     /locus_tag="OO590_002519"
     CDS             complement(9273..10019)
                     /gene="kdsB"
                     /locus_tag="OO590_002519"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6637843.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10016..10198)
                     /gene="ycaR"
                     /locus_tag="OO590_002520"
     CDS             complement(10016..10198)
                     /gene="ycaR"
                     /locus_tag="OO590_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6637844.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10250..11482)
                     /locus_tag="OO590_002521"
     CDS             complement(10250..11482)
                     /locus_tag="OO590_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6637845.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11519..12505)
                     /gene="lpxK"
                     /locus_tag="OO590_002522"
     CDS             complement(11519..12505)
                     /gene="lpxK"
                     /locus_tag="OO590_002522"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6637846.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12502..14250)
                     /gene="msbA"
                     /locus_tag="OO590_002523"
     CDS             complement(12502..14250)
                     /gene="msbA"
                     /locus_tag="OO590_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6637847.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14287..16506)
                     /locus_tag="OO590_002524"
                     /pseudo
     CDS             complement(14287..16506)
                     /locus_tag="OO590_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16713..16997)
                     /gene="ihfB"
                     /locus_tag="OO590_002525"
     CDS             complement(16713..16997)
                     /gene="ihfB"
                     /locus_tag="OO590_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6637848.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17157..18830)
                     /gene="rpsA"
                     /locus_tag="OO590_002526"
     CDS             complement(17157..18830)
                     /gene="rpsA"
                     /locus_tag="OO590_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6637849.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18941..19624)
                     /gene="cmk"
                     /locus_tag="OO590_002527"
     CDS             complement(18941..19624)
                     /gene="cmk"
                     /locus_tag="OO590_002527"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6637850.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19797..20561)
                     /gene="ycaL"
                     /locus_tag="OO590_002528"
     CDS             complement(19797..20561)
                     /gene="ycaL"
                     /locus_tag="OO590_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6637851.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4972 a         5721 c         5028 g         4979 t
ORIGIN      
        1 gcattacgcc gttatgtgcc atttttttag tgcagaaacg gagttttcgg aaaaagaaaa
       61 ggcggcattg ctgccgcctt aattttaact cgcctgggaa ggcgcttcgt ctcttcctgg
      121 aagcgtttca gggagttgcg gcgcaaatcc tcgcaggccg acgacgtgaa catgttcggt
      181 attctggaag actttacgta ccagtttata ggtggtgcct ttttccggac tgatattttc
      241 cggcgctgcg atgatgagct gcatttgcaa acgttcacac aattcaaaca gcgtggcgat
      301 agaacgagca tccagtcgcg ctgcttcatc gaggaacagc aggcggcaag gagagatatc
      361 tttaccgcgc aggcggcgag attcatcttc ccagctttgt accaccatca ccagaatcga
      421 cataccggta ccaatcgcct caccggtcga caatgcacca gactctgcgc gcaaccaacc
      481 atcggaacca cggttaacct caacttccat ttccagatag ttgcggtaat ccagcagttc
      541 ttcgccaatg gtctgcggcg tgcgctgccc catatcaatc tgcggattaa gacgctgata
      601 caatttcgcc aacgcttccg agaaggtcag acggttgctg ttaaacagat cctgatgctg
      661 ctcgtgctgt tcagagagca catccagcag catggcgtgc gtttcacgca cgttcacgtt
      721 gagacgcacg ctgttcacct gaccaaacga tacgttctgc aacccctggt tgagcatacg
      781 gatacggttc tgctcgcgct gaatggtttt gcgaatgatg ttcgccacgc tgcgggaact
      841 gatcgccagt ttctgttcac gggaggttaa ttcttcggtc agacggctaa gttcaatctc
      901 catctgttcg atagcttcca ccggatcatc ggtacgaata atatcctgac gaatacgttc
      961 acgcagatgc tgataaaccg ccacgaagaa ctgaatttta cgctccggac gtttcggatc
     1021 ttccgacatg cgcagcacgt cgcgcagatg ttcgttatcc gccaccgcca gacgcagcgc
     1081 acctaacgcc ttatccgaca tggaacgcaa atcatcagcg gagagataag ccagctcacg
     1141 acggtgtaag cggcgctcaa cgccgttatc tttcaccatg cgcatcaccg cacaccagcc
     1201 cgctttggcg gtcactacct gctcgcgcat ctcaaagtaa tcccgctcca gcttgcgcag
     1261 tttgcgggtc aggttgtcca tctccgcttc gcagaaggta agcgcttttt ccagttgatt
     1321 gcggcgtgaa cggttattgc tcagttgcgc atgcagctcg tcacggcgaa tacgcgcccg
     1381 ctcttctgcc ccgctatcag cacgcacgcc gatatcctgc aattcacgtt gcagatcgtt
     1441 gagtagctct tttttggtgt cgtaagaact tttcagcgaa gccagcacct ggttgtactg
     1501 actcaactgc gctgcgtgac cgcgcaacgc ttcgcgagcg cgggtacgct ccgcttccga
     1561 ctgttccaga cgttcacgca gtttttcgtt gagatcgctg ttaccgctaa gcatttctgc
     1621 cgagtcagaa tagctaaagt gcgcacgacg ctgcaccact tccgtcaggg caaacgcttg
     1681 ctgacgggca tcgcgctgca tctgctgaga gtacgcgtaa tcttctttta actgttcgaa
     1741 ctgttccggg tcgctctgca ataccgaaac gatcggttcc agtttcgcca gttgattgcc
     1801 aaactgctga acaaaacgcg cggcttcctg ggcttcatcc agacgttcgc ggatttcatc
     1861 gacgcgatcc gccaggctgt catcagccaa caggttgaga cgcggcagaa tgcggttcag
     1921 cgccgtaacg ccttctttcg cctgctcaaa ctgaatacgc tgctgctggt tatcattttc
     1981 atgattactc aacgcccgct ccagttcgac tcgacggctg ttcagttgac ggatttctgc
     2041 ttccgggtca gactcaaacg ccaccgccag atgactgccg ataaagcggc tgaacgcctg
     2101 atgcagacgc tgggttttct gtacatcaaa ggagagcgtg gcgaagcgtt cggaaagcac
     2161 ttcacgctcg gcatggaggc tttcaatacg gctttcacgc gcagcacgac caaacagcgg
     2221 cacttccggg aaacgtgaat aacgccactg tcgatcggcg attttcacca ccaccgcttt
     2281 ttccagctca tcaacgctga acacgctatc atcgaatgac tgcggatccc cttcgatcaa
     2341 atagagatct tccgggcaat cggtcaagcc ttccaggtgt tcggttacct gtgacagatc
     2401 tggcaccacg atggcgtggc gtgacgggcc atacagagcc gagaagtacg gcgcatcttc
     2461 caggctaacg tcgtcataaa tttctgacag cagcacacca ccaaaacgct ccgccagcgc
     2521 gttcagacgc tgatcttcag agccgccagg ctggcttaaa cgttcgatct cttcatcgac
     2581 ggcgtttttg cgcgcgccta cttcatcgcg ttcaacaatc gcctcgcgct cacgctccag
     2641 caactgttgc agatattcag tgacgtcctg gctggaggag aactcttcgc cgcactgttc
     2701 actcaactgg ttgagactgt tttgcgctgc cagccaaacc ggtgcacgca gcatcaaact
     2761 ctgaatgcga gactgcagct gttcctgttc ctgccgcagt gccatccgct cttcacgggc
     2821 gttagatacg ctatcggaaa gagaggcaat gcgtgcttcc agctcctgat gcagagcttc
     2881 cagttcatca atatcaaaat tcttgccctg acgtttgcag aaatctgcca gcagacgctc
     2941 agcttcttgc tgctcacgca gacgctgttc cagttcgctt aagcgcatcc gcaacggctg
     3001 aacctgctct gccaggtgac gctgatcaac cccttcgcgc aatagttcgc gagcgacatc
     3061 ccacgcctcg ttacgcgcca gcgggccgtt gattgccacc accagctgat aagcctgctc
     3121 aaactggctg tgcgcggttt gcgccatgct cattttctgc tcaagagaga gcattttttc
     3181 agtcgcttcc agctctttcg cctggaaggt ttccagccat tcggcggcgc tgtcggcggt
     3241 taagtccggc agatggcaca gttctttggc acgattaagc gcagcaatcg cctggttata
     3301 ctggatcgcg cgcgtttgct ggacgtccag cgcctgctgg tagtcagcaa gctggctttt
     3361 cagctcgtcc acttccagtt cggcagcttc cgcgcgagcc tcattctctt cctggcgttc
     3421 gatggcttct gccaccactt cattttgctc ttccagacgg atctgcagct catcgagatc
     3481 cacttcgtag cgttcgatct tctcctgctg acgcaatgcg gtttgcacca ggttcaggtg
     3541 atcgctggcc gcctgataat ccgcttccag atcgccttcg gcaccgttgt gctctgccag
     3601 ctcacgcgcc atatcgacgt gtttgtactg ctcagccgcc agttgctgac gcgaagtatg
     3661 tagctcgcga cgaaactcca gggctttgtc gagatggaca cggcgctcgt tggcgtgacg
     3721 catgtagtcc gccgccacgt agttggtagc ttcgctgatc agatgcttaa acaggtcacg
     3781 gtccgactgg gtgacacgaa tcgcttccag cgtcatgcgg ttttcacgca gcgccgcttc
     3841 catgtcctgg aacgctttac gcacgccgct gttttccggc aacaggtagt cgcgcagaga
     3901 acgggtaatg gcactggaga tcccgccata cagcgaagct tcgatcagac gatagaattt
     3961 gctacggtca gatgcggagc gcagacgacg cgcgatgatg cccagatcga acatcagcga
     4021 gtggtaatcg gtaatggagt tgaactgttt aaactgcacg ccttccatcg cctcgagctt
     4081 gtctttcagc tcgttaagcg gcagcacgcg cgcctggcgt tcgttcaggg tttcggtcac
     4141 cagctgtgtc ggctgcaccg acatcggcag tccctgaatg gcaaatggtt tgatatccac
     4201 tttacgatcg cgtccggcaa cctgttgcag acgcacaccg accaccacgc gctggtggcg
     4261 cgagttaatg gtgtcgagca tcgaataaca gacacccgct ttcagcttac cgtgcagacc
     4321 tttatcgcgc gaaccgctgg tggccccggc ttccgtagtg ttacggaaat gcagcagggt
     4381 caggtcgggg atcagcgccg taacgaacgc cgccatcgtg gtggatttac ccgccccgtt
     4441 accgccggaa agcgtcgtga ccagctcgtc aaggtcaaaa gttcgggcaa aaaagccgtt
     4501 ccagttaatc agcgtcagtg agcgaaattt accgcgttca atcattattc ttcctctccg
     4561 ctatctggct gactctcttc ggtttcatcg ttgagttgca gatgattttc aatcggcatt
     4621 gcttcgccat cacgaatcag gcgacgctgt gcttcacggg gatcgtcgcc agcacgcaca
     4681 tcggctccga agcggaacac cgattcggta atgcggaact tgctgctgtc gtggcccata
     4741 aaccacacca tgcctaaacg acgcagacgg ttgagcgaag aacgtacttt ctcctgcaac
     4801 ttctgacggt caacgtctga accggttgaa cggttgttca ccagtttcag cagtttcgct
     4861 tcatcggcca gggtgagcag ttcgtcgtac agttcctgct gggtgaaaat cccctcattc
     4921 gccagccgtt ccgggctgag atagagataa cagaggattt tcccgaccat catatccagt
     4981 tccgacaaga cggaacgcgg gataagagtg gtggaacgtg ggcgtaaata gaagaaccct
     5041 tccggtgcgc gaataagctc aacgttataa cgcgcgtaaa actcttccag atattcctga
     5101 aaatccatca ggaatgcatg attatccagt tcgtcgaggc caatatggcg gcctgaacgt
     5161 aaggcgctgt ccagcgccgg aaataacgga ttcgccagcg cctgcgccag cttaaccggc
     5221 atcacttgtt caatatttgt cgatgacatg cgcctgtacc ttggctccgt aatcattaat
     5281 cggctgccat ttcgctggca gtccggtgaa atctgcttgc gctacgccaa gacgtaccgc
     5341 ctgatcaata acaatacgcg caacgtcaaa gtgacgtgca cgcggatact gtgacagata
     5401 ttcgcgtacc accagaccaa gatccagcgg cacttgtctg gttttgtaca cggcaagttg
     5461 ttcttcgatg atcgccgcca gctgttcgcg gatctcgtta aactcttcgt attccagatc
     5521 ctcaggaagt tccccagtca cttcttcatc gcgcagtgcc atctcttcgt cacgcatatc
     5581 cagcagacga tcggcattgg cataagttag cgcccacggc tcatcaaaat aggtttgtac
     5641 cgactgacgt aaccgctgag caaagacgcg gtttttatcc atatcgatcg cggtacgaat
     5701 aaatttgtgt acgtggcggt cgtagccaat ccacaagtcg atggattgct ggccccaact
     5761 gataatacga tcgagtttgc tctgcagatc gaacaccaga cgatcgacga aatgcagatc
     5821 gtcatgggtc atcgtcgcat cctgaatgcg caacagatta gcctgcaatt tgtcgcctgc
     5881 cgcttccagc gtatcctgca attcacgcag cgttccggaa gtttccgaaa gcaacaattc
     5941 acagctggaa atcgccgccc gccagtcttt gttcagcaac tgggcgatat cgtccttcac
     6001 ctgctgctgc tgttcgtcca tcagacgttg cgtcaggtcg atactgtcga aaatttctgc
     6061 taccgaatat ttcagtggcg catagacatt acggtgccag tgaaattcat caccgccctc
     6121 ttcggcggca tctgctgcgc gtttgagctc acccgccaca atcgacaact gcatagaaag
     6181 acgcagcgta gaaaactcgc gctgacggat gtagtagtca gtaatgccga tgccgagcgg
     6241 cgtcagacgg taaattgcgt tcccttccgc ctgctcgctg gtaaagcggt tcagcagacg
     6301 ttgacgcacc atgtcgttga tcgcgttatt ggcgcgcacg ccgatggttt cgctggtttg
     6361 ctcaaacgca tcactcacat ggcggaatgc atccaccagc tcgccttcac tcatctcacc
     6421 atccagacgc tcgccgttca gcgtggcaac cgccagcaga aaagagagtc ggtctaccgg
     6481 cagcgagatg gagaagtcat tttttctggc ccaggcaacc agttcgggga ctgtctggga
     6541 aaattcactc atactttatc cttgctctgc ggtttgcgcg cggtgacatg aatataacgc
     6601 cctagggtaa tatacggttc ctgacggcaa taacgcgttt ctaattcaag taatgcttca
     6661 tagcagtcgc gctgctggtg cttctcgcgc agataatcat gaaacacgcg aacgcctgtc
     6721 ttacccataa tttgccaacc agcttcttcc agccacagat aaacctgcgc cgggtcgcgt
     6781 ggataatctg gcgaaagcgt ccgttttttc tttttcggca ttcccgcctg cacgtaatca
     6841 aaattcccgg cgaccatgtt atgcatcaac aaaccatgcg cattgtagaa cattaacgac
     6901 aacactctgc ctggacgcaa cactgaccac agggtctgca atacactgcg gggatcggcc
     6961 acccactcga gcaccgcatg gaacaatatc agatcaacgg gcgtttccaa atgcgaagca
     7021 acatcctgag cggcgcaatg tataaattgc atgttgtcgc tcacaccttt tgcttctgcc
     7081 gcctgttttg cgcggtcgat catctgcgca gaaagatcgc ataaaataac ctgatgcccg
     7141 cgctcggcca ttttgattgc ggtctgccct tctccgccgc cagcatccag cacacgcagt
     7201 ttttgcgggc ccatttccgc cagcacgcga tcgagatcct gccacagaat agcctgtcga
     7261 agctgccctt tggtggtgcc gtaaatgtta cgggaaaact tttccgcaat atcatcaaaa
     7321 ttgcgatcct gcattgcttt ctccaccggt tggctaagac gttttctgat aaagacgcta
     7381 ttttgtcaca gccacggcga gaatgaaccc gtctggtgta atatcgctgg cgatcccttg
     7441 ctatatggtt aaaaaaggaa ccagaaagga tgctttttac actgaaaaaa gtgattggca
     7501 atatgttgct gccacttccg ctgatgttgt taattattgg cgctggcctg gcgctgctgt
     7561 ggtttagccg ttttcaaaaa accgggaaga tttttattag cattgggtgg ctggcactct
     7621 tgttattgag tttgcaaccg gtagccgacc gcctgctgcg tcccatcgaa agcacctatc
     7681 cgacatggaa taattcgcaa aaagtggact atatcgtggt gctcggaggt ggttatacct
     7741 ggaacccgca gtgggcaccg agctctaatt taatcaataa cagtctgcca cgcctgaatg
     7801 agggtatccg tttatggcgc gaaaatccgg gttcaaaact gatcttcacg ggaggcgtag
     7861 caaaaaccaa tacggtgagt acagcggaag taggtaccag agttgcgcaa tcgctgggcg
     7921 taccgcgcga gcaaattatc accctggatt tgccaaaaga taccgaagaa gaagctgcgg
     7981 cagtgaaaca ggcgattggt gacgcccctt tcctgctggt gacctccgca tcacatctgc
     8041 cgcgggcgat gatctttttt cagcaggaag ggttaaatcc gctaccagcc ccggcgaatc
     8101 agttggccat cgactcgccg cttaatccgt gggaacgggc aatcccttct ccggtctggc
     8161 tgatgcatag cgatcgtgtt ggctatgaaa cgcttggccg catctggcag tggctgaaag
     8221 gtccgtcagg cgagccacgg caagagtgat tttgatgcca gatcgaagtt gcgccgacta
     8281 aatcgtccgg tattaaccag ttgcgcaact tcatcccata acacatacaa ccaacgccgc
     8341 cagatgaacg actccgccac tggcgcgcgt tgcaggtaac tccagagcaa atcttccgcc
     8401 agagaattat ccattagtcg gaacagttcg tattctcgcg gtgcccaaag cattagtccc
     8461 ggcccgacca tcgccagtaa ctgatcgctg cgcgaatctt tcagcatgct gcgtaaacag
     8521 aagttaccgt gaatcaacac acaattgtcg ttaaagcctt caaataatgc cgggagacat
     8581 tcgcgagtgc gaaacaggat ccgcttatcc tgcatcgtca gaccggtgtt attgaactga
     8641 ttgagcgtgg tccatagcac ttcaacatgt tgccggtacc atgagggcca gaaattttcc
     8701 tgagtattgt cgaccgcgcc gacgcaaccg cgactgtcct gacggtgcca ggccagtaag
     8761 gcttcaacga tttggtcttt gagctgttcc cagcgttctg gtgttcgggc tggtgcctcc
     8821 accgaaacac cacgcatccg ctccagtagc aggacatcag ggccggggtg ttcttcatgc
     8881 gtcatcacac cataaatagt cggcatacgg acagtcccac ttcttgccag catggtggtt
     8941 ttccatgcca gttgtcgggc ttttccgggc gtactaaagc ttcttgccat taacggcatt
     9001 gggtttccct ggctgtcata caaagcccac aacgccgtat ccgctttttc attgacgcac
     9061 tcaatacgac tgagtttttc gcccagtaaa tggcttaatt cggcacgcag ctgttccatt
     9121 cagattaccc tcataaaagc tactgctttc ataatgagcg ccaatataaa agatgtcacc
     9181 tgaatgagat caattgttga aaagaaaaat tgaggaaaat cgtaacggta cgacactcct
     9241 cccaaaattg gctgaagtgt cgtgaagtga aattagcgca tttcagcgcg aacgcgctca
     9301 agatcttcag gggtatccac acctgtgcca ggaacttcct gagcaacagc aacatggatt
     9361 ttttcgccgt accacagaac acgaagctgc tctaacattt cgatgtgttc taacggactt
     9421 gcctgccagt tgacgtaacg acggataaag cctgcacggt agccataaat accaagatga
     9481 cgcaggaagt tatcgccaac ggtttcaagg ccttctgcaa aacgatcacg atcccaagga
     9541 atggtggcac gtgagaagta cagtgcatac ccttcagcgt cgagaaccac tttcaccgca
     9601 ttcgggttaa acgcttcttc cgcattgtgg attggcaccg ccagagtcgc catacccacc
     9661 tgacgctgag cgaggttatc agcaacctga cgaatgattg tcgcagggat catcggttca
     9721 tcaccctgca cattaacgat caccgtgtcg tcgctgaatg cgcatttttc gacaacttcc
     9781 accagacgtt ctgttcctga ctgatgatcg gcgcgcgtca tacatacttc accgccagcg
     9841 gcttcaacgg cgcgggcaac atcctcatga tcggttgcca cgatgatgcg ctcggcacct
     9901 gattcacgcg cgcgttcaag aacatgaaca atcatgggtt tgccgttaat atcaaccaat
     9961 ggtttaccgg gcagacgcgt ggacgcgtag cgcgcgggaa taatgaccac aaaactcatg
    10021 atttactctc atcagcagtc agcacgcggg cttccgtttc cagcaacact ggaatgccat
    10081 cgcggagcgg gaaagccagg ttatccagtt tgcaaataag ctcttgtttt tcctggttat
    10141 accaaagttt tccgttgcaa accgggcagg caatgatttc aagcaaacga tgatccatag
    10201 ttcctccaga tggatcgggt tatgaatgca taaatcttat cataatcatt tatgcgacgg
    10261 ggtctatttc ccagccagtg cggcaatcag taaagagacc ttgcgggcag cgtcctaatg
    10321 tcacccgcgt tgcctgctgc cagctcgcga aatcagtaat cgcctgacgt aacccttttt
    10381 gcagcattgt cgttggttta atgccttcct gtaaccacag agagataact tcaaggatgc
    10441 ctgtctggcg atgcattttg gcatccattc gcccaactaa ttgcccacga tgtaataatg
    10501 gcagaacaaa atagccatac tggcgtttcg gcgctggggt atagcactcc agccggtagc
    10561 taaaatcaaa aagctgctct gcgcgtttgc gatcccagac aacaggatca aaaggcgaaa
    10621 gtaccgcgct gtgcgttgca gtgagctttc cggccagcgc acgctcgagt agcggcagca
    10681 aatcagcatg cagccaaaga ttgcccaatt tttcaacatg cacagcaatg atttgctgct
    10741 gttcagccct cgcttcgcgc cacgccgcca gtgccggacg tttcagccga tagtaatctg
    10801 ccagccactg ttcgcggaat attcccaggc tacgcgcact gttatccagc atgatgattt
    10861 ctgcttctgt ttgcgaaacg agatcgcgct catcatccca gtcaggcatg actcggtggg
    10921 ttaaatcata aacgcgctgg aagttgcgcc gttcaatcac catcaccttt ccggcagtaa
    10981 ataaaccttc cagatgccgt ttatgcggct tccattccca ccagccgctt gcacctttac
    11041 gaggatgctc aaaatcggct gaacgtaccg gccctttatc atgaatatgc tgaattaact
    11101 gtgcaatttc cgcctcatgt tcctgcatcc aggcgtcttt gtatttccag cccatttttt
    11161 caggtgccag catgcggtgg cgaataagac gaaagtcgct acgcggcata aagcaggctt
    11221 catgcgccca gtattccatt aattcgccac gcgccagaga ctcatccagc cactgggcag
    11281 gataatttcc cagacgactg aaaagcacca gatatggact acgggcaaca atattgatgg
    11341 tatcgatttg cagcaaggac atgcgggaga tcgttgccgg aatatcctcc aacgacgctc
    11401 gacggcgggg tttgtttaac aggccttgtg cggcaaggtg aagattacgc gcatcagcaa
    11461 gggagaggtg cggcagcgac attcatgact ccatcaatcg aacgctgccg cggcgtaact
    11521 agttgccaga agccagcgag gttagttgcg taagcagttt cgctggttca tcacctgaaa
    11581 gctgtgcgtc tacaggcaaa taccaccaat tttcttctgc aaaggcccgg catttcaccg
    11641 catctttttc agtcattacc agcgtttgcc cggcgcttac caacgcactg acatccgcat
    11701 ggttcaaaga ctgatgatcg gccagcggta cacatttttc cggttgtacg ccacacatct
    11761 tcagcgtggc aaaaaagcgc ggcggatgcc caatccccgc catcgccact acatgttcaa
    11821 gctgagcaac gtcacaccgc gtaccggtac gtaaattcac cgcctgaccc ggcagcagat
    11881 gcatggggat ttcaccgctg cgagggacac cgccgttgac gattaccgca tcaaccgact
    11941 ttaagcgccc cgctcgctca cgcattggcc ccgccggcaa ccaccagcca ttgccaaagc
    12001 gacgcacacc atcaataacg acaatttcca catcacgcgc cagacggtaa tgctgtaaac
    12061 cgtcgtcggt tacgatgatc tgcacatcag ggtgttgcgc cagaatggct tttaccgcat
    12121 cagaacgaac gggagaaacc gcaacaggcg catcagtgcg ttgataaatc aacacaggtt
    12181 catcacccgc ctgtgctgtg gtggtatctg ccgacaataa cagcggataa gattcagcct
    12241 taccaccata tccccgcgat acgaccccca cgcgaatacc gcgctgttgc aactgttcca
    12301 ccagccagac aacgaccggg gtttttccgt tgccgcctgc ggtgagatta ccaaccacga
    12361 caaccggtac gggggcacgc caggcgcgct tcagttttag tttatagcaa agacggatcg
    12421 cgccactcac cagaccatac aaccaggaga gtggcagcaa tagccgccac aaaggggatt
    12481 caccagacca gattttttcg atcattggcc aaactgcatt ttgtgaagtt gcgcgtaaac
    12541 gccgcggtgc tcaagcaaat cgttatgcgt accgcgttcc acaatgacac catcctcgac
    12601 gaccacgatt tcgtctgcct tttcaatggt agacaagcgg tgggcaatca ccagagaggt
    12661 acggtttttc tgcaactcat ccagtgccgc ctgaatcgca cgttcggatt cggtatccag
    12721 agccgaggta gcttcatcca gaatcagaat cgggctatca cgcagcaagg ctcgagcgat
    12781 agcaatacgc tgacgctgac cgccagagag cagcacgccg ttttcaccaa tcactgtatc
    12841 gagaccgtta tccatcttat tgatgaagtc catggcgtag gccatacgcg ccgcttcttc
    12901 aatttgctca cggctgtact gttcagtccg tgcgtaagca atgttgttag caaccgtatc
    12961 gttaaacaga tggacattct gcgacaccag agcaacctgg ttacgtaacg acgccagggt
    13021 atactcgcgc agatcgtgac catccatcag gatttcacct tcatcaatat cgtaaaaacg
    13081 cgtgatcagg ctggcaatgg ttgatttacc tgaaccagag cgtccaacca gagcaaccgt
    13141 cttccctgcc ggaattttca ggttgatgtt acgcaatgcg ggtacgtcac gtcccggata
    13201 agtaaaggtg acattgcgga attccacgtc gccagtcgca cgctcgatca cgcgcttacc
    13261 ttcgtctttc tcctgctcac tgtccagaat ggtaaacagc gtctgacaag ccgccatacc
    13321 gcgctggaac tgggcgttaa cgttggtcag cgatttcagc ggacgcatca gtgcaatcat
    13381 tgaagagaaa acaacggtaa tcgtaccggc agtcaggtta tccataacac ttgggaagct
    13441 cgccgcatac agaacaaacg ccagcgccaa agaggcgatc agctgaatga tcggatcaga
    13501 gatggaagag gctgaaacca ttttcatccc ctgaagacgc attcggttac tgactttatc
    13561 gaagcgtttc gtttccactt cctgaccacc gaaaatcaat acttctttgt ggcccttcag
    13621 catttgttct gcactggtgg tcacttgccc catggtgttc tgcatgtttt tactgatgtt
    13681 gcgaaaacgc ttcgatacta cgcgaatcgc aatcgaaaca atcggtgcca gcacaatcaa
    13741 aatgatcgac agttgccaac tgtaatagaa catcatgatg aacaggccga tgatcgacgc
    13801 accttcacgc acaacagtaa tcagtgcgcc ggaagaagaa gaagcaacct gttcggaatc
    13861 gtaggtgata cgtgacaaca gcgtacccgt tgactgtttg tcaaagaatg aaactggcat
    13921 tcccatcatg tgaccaaaca ggcggcgacg catggtcatt accacctttc ctgataccca
    13981 ggagatacag tagctggaga catagctggt gataccacgt aaaatcatca gtccgatcac
    14041 caccagcggc atccacacca gcacggagcg atctgtttta ccaaagccat catcaagaag
    14101 tggcttaagg agcgataaca tgaaggtatc gctggctgcg ttgaggatta acgctacgcc
    14161 cgccacgatc agacccgctt tgaaaggcgc aatggttggc cacagtcggc ggaatgtctg
    14221 ccacgtagag agatctttgt cgttatgcat tcaaaaaacc agcatttgtt gaaatagccg
    14281 catattctac ccgttatcca ctggcacgcc aaaccactga taataccaac gaggtaaaat
    14341 ttgctctctg aggctgctaa tccgccagcc ttgtgctgaa aaattgaccg tcacttgacc
    14401 ctgatgtgga gtatcaagcc attgatatcc tttctgttga tagcgatgct taactttgtt
    14461 agagggcaat cgccatgcgt tatagcgcga tgccgatgcg agtgccactt ttccattcac
    14521 tcgctgaatt aatggcaacg atgatgaggt attactgcca tggtgaggta cctgaagcaa
    14581 tgttgcctgc acttgctgcc agtaacggct tagcatcttt tgttcagctg gggcttcaat
    14641 atcaccggtt agaagaatgc tattcgtccc gtcatcaacc ttaaccacac aggaatggtt
    14701 atttccctta tcgttgctac cttgtaaagg ccagtgcgcg ctgaaacgca atccttgcca
    14761 ctgccacgct tcgccacgca cacagggctg atgatgttcc cagtttaacg gacttctgat
    14821 ccataacatc ggctatatat gcaatattga atccagccct ccccggtgat ccagatgttc
    14881 atggctcaga ataacgcctt ccggttcaag attatgccag tggagccagg ggataatcag
    14941 ttgttgccca ctatcccctt caggccaggc cagtcctgtg tcatagagaa tcgctttgcc
    15001 gtttctggct atcaccattg ccagcccttg cccgacatca agcatgtaca gctgccactc
    15061 gtcaggtcga ggtttttgcc acagcggcca gcacatcagc aagcctccag ccacacacat
    15121 tactggcaac gttcgccagg cgtttaatcg ccataccacc agtaagaacc atggggaaaa
    15181 tgatagccat tgccaacatt caacaatgtt gatccaccct tccggcaaac tctttaaccc
    15241 ctgttccagg attaacggcc cgctaaaatg cacaaccatc gcggcgagga tcaacggaac
    15301 cgtgataaat gtcaccaagg gaattgcaaa tagatttgca ataaacgagg tcagactaat
    15361 gccatgaaat atgacgattt gcatgggcat aagcaggagt gtgattccca gttgcagatg
    15421 gatgagggaa acaactgcac gcaataccgg cggcagttgc cactcaggac agggaaacca
    15481 ctgataccaa aaaatcagtg ccgcgaccgc agcggcagag agccataaac tttgcgagag
    15541 aatggcaaca ggatccatca gcaaaattgc cgccagacaa catatccata catcccagcc
    15601 actccactgt cgcccactta acttaagcat tccccacgta gcaagcgcca ccacggtacg
    15661 caatgcagga ggttgcattc ccgtcaacta ggcataaaaa gcagcacagc agattccgcc
    15721 aattaatggt atttgccagt ggatccagcg cccaggcaga aaaatttgtc cactgcgaat
    15781 gagtccggca gccagcaacg ccgcaaaagc gatgtgcaat cccgatatcg ccattaaatg
    15841 cgccgttcca gtatcgcgca tgatattttt gatttctttg gggacggata accgttcccc
    15901 catacctaaa ccaagaataa ccgcattcca cggatagggt tgcagcgttg tttgtagtga
    15961 cgccagatac tgtgcacgca ggctacaatt cggttcaatg acacttgcct gcagaaaacg
    16021 gccggtgagc ggctgatgct gggcaatggc ataacgctgg ctatcaaagc cgccatcatt
    16081 aagttggcca tgaactgcac gaactttgag tttcattgac cattgttgtc cagcacaaac
    16141 cgcttgcgga agttattcgc catacagcac aaggcctggc gcagggaata tacgtcgacc
    16201 ttgtagataa gtaatttgac cgtaatgagt ggtcatgccg tcagttgcag tgatctcaac
    16261 aattgcatcc tgcgtcgcgc cagttaaggt ttctcctgcc cacaaaattt gctttgctga
    16321 taatatgccc caaacgaaaa acagcaacgt cagcgcgata taacggacgg ttttaacagg
    16381 gataaatgcc agtacgcagg cgaagagagt cagaaacgca agggttaatg tcctaggcaa
    16441 ttggggcaaa atcagcaacg gaaaaattcc gcaaattatg catacgccga ctgtcgttat
    16501 tttcatgccc ccctccctgt ttatggaaag tgtgcaactt tgttgagcgg ttgtctggtg
    16561 ggaattttgc gttccccgag gcacattcag aagaatttaa cgaaagggtg aaaactgctc
    16621 aaaactctgc gaggcagctt ccagttcctg attagagaat tgaaaaaagc acccgacagg
    16681 tgcttttctc gcgttcaagt ttgagtaaaa acttaaccgt aaatattggc gcgatcgcgc
    16741 agttctttac caggtttaaa gtgaggaacg tattttcctt ccagttctac tttatcgcca
    16801 gtcttcggat tacgtccggt acgtggtgcg cggtagtgca aagagaaact gccgaaaccg
    16861 cggatttcaa tacgctcgcc ctgcgcaaga gtcgaggcca tatgctccag catctctttt
    16921 actgcatctt caaccgtctt ggcgggaatg tgcgattgct gggtggcaag tctttctatc
    16981 aattctgact tggtcatgat tcctccggtt ccttaaaggc aaattaagcg gctgctgttg
    17041 ctgcattgat taagggcggc cgtagccgcc cttagtgctt gattacagga cgaaacctgc
    17101 aatctgtcaa gtaaactcaa caaacttcgg aataaaaatc ccgaagagtc agagaattac
    17161 tcgcctttag ctgctttgaa agcttcagcc attgcgttgt tggagaagtt tgcatcttcc
    17221 tgtttgttaa cagttgcgat tgcatctttc tcgtcagctt cgtctttcgc acgaacagac
    17281 aggctgattg cgcggttttt acgatcaacg ccggtgaatt tagcttcaac ttcgtcgcca
    17341 acgctcagaa ccagggtagc gtcttcaacg cggtcacggg atgcttcaga agcacgcagg
    17401 taaccttcaa cgccgtcagc cagttctacg gttgcgcctt tagcgtcaac tgcagttact
    17461 ttaccggtta cgatagcgcc tttcttgttc agagcaaccc agttgttgaa cggatcttct
    17521 gcgagctgtt taacgcccag ggagatacgt tcacgttctg cgtcaacctg cagaacaact
    17581 gcagcgattt cgtcgccttt tttgtattca cgaactgctt cttcgcctgc aacgttccag
    17641 gagatgtcag acaggtgaac caggccgtcg atgccgccgt ccaggccgat gaagataccg
    17701 aagtcagtga tagacttgat tttaccttca acacggtcgc ccttgttgtg ggtttccgcg
    17761 aactgctgcc acgggttagc tttgcactgt ttcagcccta gggagatacg acgacgttct
    17821 tcgtcgatat ccagaaccat aacttccact acatcgccaa cgttaacaac tttggacggg
    17881 tggatgtttt tgttggtcca atccatttcg gaaacgtgta ccagaccttc aacgccttct
    17941 tcgatttcaa cgaagcagcc gtagtcagtc aggttggtca cgcgaccagt cagtttggta
    18001 ccttccggat aacgtttagc gatagctacc cacggatctt cgcccagctg tttcaagccc
    18061 agggatacac gggtacgttc gcggtcgaac ttcagcactt taacagtgat ttcgtcgccc
    18121 acgttgacga tttcgctcgg atgcttaacg cgtttccagg ccatgtcagt gatgtgcagc
    18181 aggccgtcaa cgccgcccag atcaacgaat gcaccgtagt cagtgaggtt cttaacgata
    18241 cctttaactt ccatgccttc ctgcaggttt tccagcagct gatcgcgctc tgcgctgttt
    18301 tcggattcga taacggcacg acgagaaaca acaacgttgt tgcgcttctg atccagcttg
    18361 attactttaa attcaagctc tttgccttcc aggtgcagag tgtcacgcac cggacgaacg
    18421 tctaccagag aaccaggcag gaacgcacga ataccgttca gctcaacagt gaagccgccc
    18481 ttaactttgc cgttgataac accggtaaca gtttcagcat cttcgtaagc tttttccagc
    18541 gtgatccagg cttcgtgacg tttagctttc tcacgggaca gcagagtttc accgaagccg
    18601 tcttctactg cgtccagagc aacgtcaact tcgtcaccta cctggatttc cagctcgccc
    18661 tgggcgtttt tgaactgctc agccgggatg gcggactcag atttcagacc agcgtcaacc
    18721 agtactacgt ctttgtcgat agcaacaaca acgccacgaa cgatagaacc cgggcgggtt
    18781 tcgatttctt ttaaggactc ttcaaagagt tgagcaaaag attcagtcat gtttaatctt
    18841 caggtttata tttaacgtcc acctggctcc atgccggatg gggttgttta acatacccgc
    18901 tgtcattcca ttgcaacggg ggtactgcaa attcggtcgc ttatgcgaga gccaatttct
    18961 ggcgcgcgta ttgtagcgct ttttcaatca cttgctcaat gcttaaggtg gtggaatcca
    19021 acactaaagc atcggctgcc ggaaccagtg gcgctaccgc tcggttacga tcgcggtcgt
    19081 cgcgttcttt gatctcggcc aaaaggcgct cgaagttaac actaaagccc ttctcctgca
    19141 actgtagcat gcggcgatgc gcacgttctt ccgaggaggc gtcaaggaaa attttcactg
    19201 gtgcatcagg gaataccacc gttcccatgt cgcggccatc ggcaatcaga cctggtaatt
    19261 cgcgaaacgc gcgttggcga cgcaataatg cttcacgaac gcgtgggaat gccgcgactt
    19321 gtgaagccgc attcgccact tcctgagtac gaatttcgcc gctgacatct tccccttcga
    19381 ggatcacttc cagattgcca ttggtcgaca caaaacgtac atccagatgg gatgccagcg
    19441 gtaccagcgc atcttccgac gcaacatcaa catggtgatg taatgccgcc aatgccagta
    19501 cgcgataaat tgcacccgag tccagcagat gccattgcaa cgcttccgcc atagccttac
    19561 acaaggtgcc tttccctgca ccgcttgggc catcaatggt aataaccggg gcaattgccg
    19621 tcatctttat ctccttaaaa caggcgtacc gttaacataa ccgcgcgcat tatacgcgcc
    19681 gacgccacaa atcgttaacg ttgtgtgcaa aaaccagttt tgtctgtatt cagatgggga
    19741 tttattgcgc aattatagcg ggctggcaga agaccagccc gaaagatgac aatgatttac
    19801 ttaccagagg cgatacgatc acggatgtgt tgcgcacgct ctgttgatgg tgggtgagag
    19861 tcaaacatgg actgggtgcg accgccatcc aggctagcca gtttttcaaa gctgccaacc
    19921 agcccctggg tgctgatgcc acgtttcttc aacagatcaa aagagaaatc atctgcttcg
    19981 gactctttat cacgggagta tttagcatta atagcgcctt ctgcgatatc acccaattgt
    20041 gagcgggaaa gctgggaagc cacgccgctg gtagctgaaa tggcatcgcg tgcggcaacg
    20101 atcgcataag aagctttcat ttcagccagc gagtgaccca acgcgacgtg gcccagttca
    20161 tggcccagaa caccttcaat ttcgttgtca ttcatcatgt ccatcaggcc actgtagaca
    20221 cgaacacagc cgttcgccat cgcccatgcg ttgacgtcgc tggtcatata aaccttatag
    20281 ttgaccggcg tgccattaat attgttaccc aatgctttgg cgatttttgc cagacgttta
    20341 ccgtacttgc tggatttact tgcgacttga ttgccgctgt ccatttcggc acagccctga
    20401 ttggcaatcg cttttgcatc ggcatcgctt aatgttgctg ctttataggc atttaaaccg
    20461 gagctgatag ccatattggt atcaataccg tgggtatttt gacaccctgt cagtaatgct
    20521 gcagaggtcg caatcgccag cagtaattta gtattcttca tgtgaatcat ccgttgatta
    20581 atcatatata aatattatgt atcaacaact tacattgaca agcgacgcta ccattaatca
    20641 aaatgactca caaggtccga aaaaaaacgc cgacaatgtg ccgtaatgcc agtcagttaa
//
LOCUS       DAJTYF010000068        20607 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTYF010000068 DAJTYF010000000
VERSION     DAJTYF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.068"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            145..297
                     /gene="hokA"
                     /locus_tag="OO590_002529"
     CDS             145..297
                     /gene="hokA"
                     /locus_tag="OO590_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6637852.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(484..696)
                     /gene="cspA"
                     /locus_tag="OO590_002530"
     CDS             complement(484..696)
                     /gene="cspA"
                     /locus_tag="OO590_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6637853.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(977..1267)
                     /locus_tag="OO590_002531"
     CDS             complement(977..1267)
                     /locus_tag="OO590_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR6637854.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1701..2411
                     /locus_tag="OO590_002532"
     CDS             1701..2411
                     /locus_tag="OO590_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6637855.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="OO590_002533"
     CDS             complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="OO590_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6637856.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3539..4198)
                     /locus_tag="OO590_002534"
     CDS             complement(3539..4198)
                     /locus_tag="OO590_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6637857.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGVLSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4351..6684
                     /gene="bisC"
                     /locus_tag="OO590_002535"
     CDS             4351..6684
                     /gene="bisC"
                     /locus_tag="OO590_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6637858.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6653..7093)
                     /locus_tag="OO590_002536"
     CDS             complement(6653..7093)
                     /locus_tag="OO590_002536"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6637859.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7090..7653)
                     /gene="tag"
                     /locus_tag="OO590_002537"
     CDS             complement(7090..7653)
                     /gene="tag"
                     /locus_tag="OO590_002537"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6637860.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7811..8509
                     /locus_tag="OO590_002538"
     CDS             7811..8509
                     /locus_tag="OO590_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6637861.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8738..9946
                     /locus_tag="OO590_002539"
     CDS             8738..9946
                     /locus_tag="OO590_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6637862.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10270..11961
                     /gene="eptB"
                     /locus_tag="OO590_002540"
     CDS             10270..11961
                     /gene="eptB"
                     /locus_tag="OO590_002540"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6637863.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASAMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12053..12129
                     /locus_tag="OO590_002541"
     tRNA            12053..12129
                     /locus_tag="OO590_002541"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12087..12089,aa:Pro,seq:cgg)
     gene            12873..14480
                     /gene="dppA"
                     /locus_tag="OO590_002542"
     CDS             12873..14480
                     /gene="dppA"
                     /locus_tag="OO590_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6637864.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FESVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14788..15807
                     /gene="dppB"
                     /locus_tag="OO590_002543"
     CDS             14788..15807
                     /gene="dppB"
                     /locus_tag="OO590_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6637865.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15817..16719
                     /gene="dppC"
                     /locus_tag="OO590_002544"
     CDS             15817..16719
                     /gene="dppC"
                     /locus_tag="OO590_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6637866.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16730..17713
                     /gene="dppD"
                     /locus_tag="OO590_002545"
     CDS             16730..17713
                     /gene="dppD"
                     /locus_tag="OO590_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6637867.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17710..18714
                     /gene="dppF"
                     /locus_tag="OO590_002546"
     CDS             17710..18714
                     /gene="dppF"
                     /locus_tag="OO590_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6637868.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18744..20015)
                     /locus_tag="OO590_002547"
     CDS             complement(18744..20015)
                     /locus_tag="OO590_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6637869.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20491..20598
                     /locus_tag="OO590_002548"
     CDS             20491..20598
                     /locus_tag="OO590_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6637870.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4931 a         5100 c         5422 g         5154 t
ORIGIN      
        1 tccaattgaa tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg
       61 gtcagtacaa agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt
      121 ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg
      181 atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat
      241 attaaacagg agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag
      301 tcttcggcgg gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac
      361 atttattctt cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga
      421 aaaaaaataa aaaaatcccc gcaaaatggc agggatctta gattctgtgc ttttaagcag
      481 agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa
      541 ggacactttc tgaccttcgt ccagagattt gtaaccatag ttctggatag cagagaagtg
      601 tacgaacaca tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt
      661 gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt aataattaag
      721 tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact
      781 acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc
      841 tgtacgtcaa accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt
      901 tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa
      961 taaagtggat aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca
     1021 aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta
     1081 ccatagcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt
     1141 ttcgtaaccg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg
     1201 tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat
     1261 attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta
     1321 agaatagtcg ggaaaagcag acacagcggg gttaagtcag ggcatgctct ggctatgatt
     1381 cgattcagcc tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata
     1441 tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta
     1501 aatctctggc tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt
     1561 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1621 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1681 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1741 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1801 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1861 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1921 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     1981 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2041 tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2101 ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca
     2161 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt
     2221 tcacgcaaca actggtgatg gttggagact acattgctca gcaaggtact caggtgagct
     2281 ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga
     2341 ttgcgccatt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2401 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2461 ttagtccgcg acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2521 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2581 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2641 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2701 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat
     2761 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2821 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2881 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     2941 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3001 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3061 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3121 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3181 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3241 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3301 ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg
     3361 aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3421 aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata
     3481 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3541 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3601 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3661 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3721 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3781 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc
     3841 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3901 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     3961 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4021 cgcgatcttt cttcgaagaa gagagcacac caatacccgc gcccacgaga gagcccagac
     4081 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4141 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4201 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4261 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4321 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4381 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4441 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4501 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4561 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4621 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4681 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4741 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4801 gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc
     4861 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4921 aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca
     4981 ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat
     5041 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5101 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcat
     5161 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg
     5221 aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg
     5281 gcggctattt tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa
     5341 cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc
     5401 ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg
     5461 cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg
     5521 gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5581 cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc
     5641 aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg
     5701 gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct
     5761 gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     5821 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5881 tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa
     5941 agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     6001 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     6061 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg
     6121 catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac
     6181 ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6241 gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgtct gcacagccag
     6301 ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt
     6361 catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac
     6421 tcccgtgggc agatccttgc tggagcggtc attagcgagg gaattaaacc tggcgtgatt
     6481 tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac
     6541 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6601 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6661 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6721 aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat
     6781 aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa
     6841 taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     6901 tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa
     6961 gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat
     7021 gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7081 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7141 cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccaca
     7201 ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa
     7261 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa
     7321 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7381 tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7441 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7501 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7561 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7621 cgggccctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7681 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7741 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7801 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7861 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7921 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     7981 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8041 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8101 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac
     8161 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8221 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8281 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8341 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8401 tatgttgctc aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac
     8461 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8521 caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa
     8581 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8641 tgatggcatt tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc
     8701 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8761 tacccgctgg ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta
     8821 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     8881 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     8941 attgcaggaa cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt
     9001 aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     9061 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     9121 gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag
     9181 cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt
     9241 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     9301 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     9361 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat
     9421 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9481 tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct
     9541 ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt
     9601 gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt
     9661 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact
     9721 ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
     9781 tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt
     9841 gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg
     9901 tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag
     9961 ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact
    10021 cgtaaaattt gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct
    10081 tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc
    10141 gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac
    10201 ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg
    10261 tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca
    10321 atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg
    10381 cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg
    10441 gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg
    10501 gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg
    10561 gtcattggtt atggcatcat cgcttccgcc atgaccaccg atatcgacct gtcaaaagaa
    10621 gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt
    10681 atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt
    10741 attcgcagcc tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg
    10801 ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc
    10861 ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc
    10921 tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt
    10981 acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc
    11041 acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc
    11101 caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca
    11161 ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa
    11221 gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg
    11281 cagagcgaaa tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag
    11341 attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa
    11401 atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa
    11461 ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa
    11521 tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg
    11581 gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg
    11641 attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc
    11701 acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca
    11761 gataaatatc tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc
    11821 gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc
    11881 tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca
    11941 aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
    12001 ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgccccgca ttcggtgatt
    12061 ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat
    12121 caccgaccaa attcgaaaag cctgctcagc gagcaggttt ttttgcattt gtagctcatg
    12181 aggatgagaa cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc
    12241 agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc
    12301 gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac
    12361 ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact
    12421 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
    12481 atatccacct tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatttt
    12541 gctaaatatt aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa
    12601 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
    12661 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
    12721 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
    12781 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
    12841 aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga
    12901 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
    12961 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
    13021 gcaccaccta tgacgcctct tccgtcccgc tttataaccg cctggttgaa tttaaaatcg
    13081 gcaccaccga agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
    13141 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
    13201 cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
    13261 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg
    13321 agctgatcag cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac
    13381 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
    13441 atgctgacgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa
    13501 ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg
    13561 atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg
    13621 cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc
    13681 cggcagacat cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc
    13741 tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc
    13801 gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
    13861 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
    13921 aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggcctggaaa
    13981 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
    14041 gccgcatggc ggagatgatt caggcagact gggcaaaagt gggcgtgcag gccaaaatcg
    14101 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa
    14161 tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct
    14221 gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc
    14281 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
    14341 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
    14401 aatcggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg
    14461 aaaacgtctc tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc
    14521 tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt
    14581 tgtaggccgg ataaggcgtt aacgccgcat ccggcataaa caaagcgcac tttgtcaaca
    14641 atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca
    14701 ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc
    14761 tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc
    14821 gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg
    14881 ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag
    14941 ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg
    15001 ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag
    15061 ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg
    15121 gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac
    15181 actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg
    15241 ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat
    15301 atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc
    15361 tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg
    15421 ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg
    15481 ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt
    15541 atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg
    15601 ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg
    15661 cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg
    15721 ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg
    15781 aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa
    15841 gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc
    15901 aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt
    15961 gccaactgga ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg
    16021 ccagcctggc aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt
    16081 gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg
    16141 gtggtgttgt cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc
    16201 ctggttgata acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg
    16261 ttggcactgg tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg
    16321 accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg
    16381 aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt
    16441 attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct
    16501 aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg
    16561 cctgagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc
    16621 gtgaccttcc ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac
    16681 ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt
    16741 aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga
    16801 ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc
    16861 cggtaagtcg gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat
    16921 ggcagaaaaa ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg
    16981 caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc
    17041 atgctacacc gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa
    17101 aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc
    17161 ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat
    17221 cgccatggcg attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct
    17281 ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa
    17341 catggcgctg gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat
    17401 cattgtgatg tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc
    17461 gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa
    17521 agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg
    17581 cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct
    17641 gaatatgctc gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag
    17701 gcccacacta tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac
    17761 ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg
    17821 ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct
    17881 ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc
    17941 gagctgtatt atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt
    18001 cggcagaaaa tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa
    18061 gttgggcaaa ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt
    18121 cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc
    18181 tatccgcaca tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg
    18241 ctcgacccgg atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt
    18301 gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt
    18361 atctcccacg acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta
    18421 ggccgctgcg tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac
    18481 acccaggcgc tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc
    18541 aaactcaccg gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc
    18601 cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga
    18661 ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca
    18721 aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc
    18781 aaaatgccgt aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg
    18841 actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc
    18901 aactgaaaga acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg
    18961 ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt
    19021 cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca
    19081 aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt
    19141 ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg
    19201 gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg
    19261 agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct
    19321 gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa
    19381 aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag
    19441 gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc
    19501 cctactttga ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac
    19561 gcaatcgcta tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg
    19621 gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag
    19681 tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag
    19741 ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa
    19801 agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg
    19861 gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca
    19921 ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat
    19981 ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg
    20041 cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc
    20101 agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac
    20161 aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca
    20221 aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt
    20281 aaaaagcaat cgtaaaatca gcggcaagcc ggaagggttc cggtgaggcg caatgttgcg
    20341 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    20401 atgtacctga caacataacg ggggctaatc ttgaacctag accactcaag aatagccgcg
    20461 aaacgttgtc attacaacac aggcggctat atgacgctcg cagagctggg catggccttc
    20521 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
    20581 ctgaacaagc ggaagtagcg tgtcatg
//
LOCUS       DAJTYF010000069        20516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTYF010000069 DAJTYF010000000
VERSION     DAJTYF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.069"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO590_002549"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO590_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6637871.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO590_002550"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO590_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR6637872.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OO590_002551"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OO590_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6637873.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO590_002552"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO590_002552"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6637874.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFCKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO590_002553"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO590_002553"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6637875.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4448..4813
                     /locus_tag="OO590_002554"
     CDS             4448..4813
                     /locus_tag="OO590_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6637876.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4963..5481
                     /locus_tag="OO590_002555"
     CDS             4963..5481
                     /locus_tag="OO590_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6637877.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5471..6696
                     /gene="dgcN"
                     /locus_tag="OO590_002556"
                     /pseudo
     CDS             5471..6696
                     /gene="dgcN"
                     /locus_tag="OO590_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6712..7194
                     /locus_tag="OO590_002557"
     CDS             6712..7194
                     /locus_tag="OO590_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6637878.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQLQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="OO590_002558"
     CDS             complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="OO590_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6637879.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="OO590_002559"
     CDS             complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="OO590_002559"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6637880.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="OO590_002560"
     CDS             complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="OO590_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6637881.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="OO590_002561"
     CDS             complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="OO590_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6637882.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="OO590_002562"
     CDS             complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="OO590_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6637883.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10938..11729
                     /locus_tag="OO590_002563"
     CDS             10938..11729
                     /locus_tag="OO590_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6637884.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11750..13036
                     /locus_tag="OO590_002564"
     CDS             11750..13036
                     /locus_tag="OO590_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6637885.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="OO590_002565"
     CDS             complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="OO590_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6637886.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13807..14685
                     /gene="nadK"
                     /locus_tag="OO590_002566"
     CDS             13807..14685
                     /gene="nadK"
                     /locus_tag="OO590_002566"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6637887.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14771..16432
                     /gene="recN"
                     /locus_tag="OO590_002567"
     CDS             14771..16432
                     /gene="recN"
                     /locus_tag="OO590_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6637888.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16581..16922
                     /gene="bamE"
                     /locus_tag="OO590_002568"
     CDS             16581..16922
                     /gene="bamE"
                     /locus_tag="OO590_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6637889.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16984..17274)
                     /locus_tag="OO590_002569"
     CDS             complement(16984..17274)
                     /locus_tag="OO590_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6637890.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17264..17701)
                     /gene="ratA"
                     /locus_tag="OO590_002570"
     CDS             complement(17264..17701)
                     /gene="ratA"
                     /locus_tag="OO590_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6637891.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17872..18354
                     /gene="smpB"
                     /locus_tag="OO590_002571"
     CDS             17872..18354
                     /gene="smpB"
                     /locus_tag="OO590_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6637892.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18569..18931
                     /gene="ssrA"
                     /locus_tag="OO590_002572"
     tmRNA           18569..18931
                     /gene="ssrA"
                     /locus_tag="OO590_002572"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19136..20377
                     /locus_tag="OO590_002573"
     CDS             19136..20377
                     /locus_tag="OO590_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6637893.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDDDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5297 a         5362 c         4815 g         5042 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac catcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcgct ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcag aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccaa gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc agtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcattaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat atataaattt acaccccttt gaaaacaatc cgctatgctt tgaaaaagga
     4441 gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct ttactggtta gcggatgtca
     4501 gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat
     4561 ggaagatgcg atatcggaca gcgccataaa agatgacgat gcatttagcg attcacaggc
     4621 ggatcgcggt ctatacctta aaggatatgc cgaagggcaa aagaaaactt gccagaccga
     4681 ttttacttat gcccggggac tttccggtaa aagctttcct gccagctgca acaatgttga
     4741 aaatgccagc caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat
     4801 acgattaaat taattaaaac cccacaaata aattaaacat aagattttac tcattgtgtt
     4861 ttatttctca cattgatgac ggtcgcatga aaaaatgata ttttaatgag catcctgaaa
     4921 aaatatcgat gcatttcgag cgaagatggt ggggatccct gaatgcgttt ttctcaccga
     4981 ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca agagccttct
     5041 gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac ccgctggcct
     5101 gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag taccgcccta
     5161 caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac tcaacaagag
     5221 gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac attccagatg
     5281 gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca aaataccgag
     5341 tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag atttgccgta
     5401 aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt aatgctcgca
     5461 cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt ttaaaagagc
     5521 attacgcaac atcagtatga ccagcatatt tatcactatg atgttgatct ggttgctgct
     5581 ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc
     5641 agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc ctgcagcaac
     5701 tgaaacactg gcagcgctgg gccagcaagg gcattttcaa ctgcagaagt acgtgataag
     5761 cagcaaaata ttctggcatc ctggcattac ccccgtaagg atccaggcga tactttcagc
     5821 aatttcataa gccactggct cttccctgcc cccatcattc agccgattcg tcacaatggt
     5881 gaaaccattg gcgaagtacg cttaaccgct cgcgacagtt caattagcca ttttatctgg
     5941 ttttcactcg ccgtactgac cggttgtatt ctgctggcat caggcatcgc aattaccctc
     6001 acccgccatt tgcacaatgg cctggtggaa gcactgaaaa atatcaccga tgtcgtacat
     6061 gatgtgcgtt ccaaccgcaa tttttcccga cgagtttcgg aagaacgtat cgctgagttt
     6121 caccgcttcg ctctcgactt caacagtctg ctggatgaaa tggaagagtg gcagcttcgt
     6181 ttacaggcca aaaatgcgca gcttctgcgt accgcgctac atgacccatt aaccgggctg
     6241 gctaaccgcg cagcgtttcg tagcggcatc aatacgttga tgaacaattc cgatgcccga
     6301 aaaacgtcgg cgttactatt tcttgatggc gataatttca aatatatcaa tgatacctgg
     6361 ggtcatgcga cgggcgatag ggtcttgatt gaaatcgcaa aacggttagc tgaatttggc
     6421 gggctgcgac ataaagcata ccgcctgggc ggcgatgaat tcgctatggt gctctatgat
     6481 gtacagtcgg aatctgaagt gcagcagata tgctcagcac tgacacaaat ctttaatctc
     6541 ccgtttgatc ttcataatgg ccatcagacc accatgacat taagcattgg ttacgcgatg
     6601 accattgagc acgcctctgc ggaaaaatta caagagcttg ccgatcacaa tatgtatcag
     6661 gccaaacacc agcgtgccga aaagctggtg agataacaag gatatatcga tatgataaag
     6721 cacctggtag cacccctgat tttcacctca ctaatactga ctggctgcca gtcccctcag
     6781 ggaaagttta ctcctgagca agtcgccgct atgcaatctt atggatttac tgaatccgcc
     6841 ggcgactggt cgctgggctt atcagatgcc attctgttcg caaaaaatga ctacaaattg
     6901 ctcccggaaa gccagcaaca gctccaaacc atggcagcca aattggcctc gacagggcta
     6961 acacatgccc gtatggatgg acacaccgat aactatggtg aagacagtta caacgaaggc
     7021 ttatcattga aacgggcgaa tgtcgtggcc gatgcatggg ctattggtgg acaaattcca
     7081 cgcagcaatc tcaccacaca gggtttagga aaaaaatatc ccatagccag taacaagacc
     7141 gcccagggcc gcgccgagaa ccgccgcgtc gcagtggtga ttacaacccc ttaaaacacg
     7201 agcaaacaga caaaaaaagg gccagccaat tgcccagccc ttcttaacag gatgtcgctt
     7261 aagcgaaatc ttagttaaga cgctctttga tacgagcagc cttaccagta cgctcacgca
     7321 ggtagtacag tttagcttta cgaacagcac cacgacgttt gacagaaatg ctgtcaacta
     7381 ccggagagtg agtctggaag acacgctcaa cgccttcgcc gttggaaatt ttacgaacag
     7441 tgaatgcaga gtgcagaccg cggttacgaa tagcgataac cacgccctcg aatgcctgca
     7501 gacgtttttt ggaaccttca acaacccata ctttcacttc cacggtatca cccggacgga
     7561 aggaaggtac gtcctgcttc atctgctctt gttcaagttg cttaataatg ttgctcataa
     7621 tttaatctct tatcctgggt aaactgatct ctcgggggct tacgccatcc catcatgttt
     7681 atgttgctgt tgtgcgtgtt ccgttttgaa ctccgccagc aaccttgctt gctcttcagt
     7741 cagagccagg ttttccagaa gttcaggtct tctaagccag gtacggccca gcgactgttt
     7801 caaacgccag cgacgtatct cggcatggtt gcccgacagt aacactggcg gaacttccat
     7861 cccttctaac acctcaggcc gcgtatagtg cgggcaatcc agcaatcctt cagcaaagga
     7921 atcttccgtt gccgaggctt catgtcccag tactcccgga ataaaccggg aaacggagtc
     7981 aatcagcgtc attgctggta actcaccacc actgagaacg taatcgccga ttgaccattc
     8041 ttcgtcaatt tcggtttgga tcacgcgctc atctatacct tcgtagcgac cgcacaccag
     8101 aatcaatttt tgattcgttg ccagttcgct gacgcccgct tgatcaagct tgcgtccctg
     8161 tggtgacaga taaatcacct ttgcgccttc acccgccgcg gcttttgctg catgaatggc
     8221 gtcccgcaag ggttgcacca tcattaacat ccccggtccg ccgccgtaag gacgatcgtc
     8281 cacggtacgg tgccggtcat gcgtgaagtc gcgaggactc caactctgga tgctcagcag
     8341 gccattttta actgcccggc cagttacccc gtaatcggta attgcgcgga acatttcagg
     8401 aaacaggcta attatgccaa tccacatagc gccgtctttt accgtttatc cggtggttta
     8461 aaaaccagga tcccaatcta cttcgattga acgagtagtg agatcgactt tcttgataac
     8521 ctgcccatcg aggaacggta cgagacgttc cttgatacca aacgcatctt tcaggtttgc
     8581 cttaatgacg agaacgtcat tagatccggt ttccatcata tcgacgactt taccgagatc
     8641 gtagccttca gtggttacta cctggcagcc catcaggtct ttccagtagt agtccccctc
     8701 ttcaagctga ggcagctgcg atgaatccac gacaatttca caattcgtca gcaggttcgc
     8761 cgcatcacga tcgtcaacgc ctttcagctt gatgatcatg tcctgattgt ggtgcttcca
     8821 gctttccagc tggacttgct gccactgacc cgccttctgg ataaaccagg gctgatagtc
     8881 aaaaatgctt tcggcgtctt cggtggaaga aaacactctg agccacccac gaataccgta
     8941 agacgaaccc atttttccca aaacgatggg atcaacaggt gcttgcgcgg tgagttgttt
     9001 gctcatcatg accaccgtga cagattaagc tgctttgttt acttctttga tcagcgcagc
     9061 aacgcgatca gaaatagttg cgccctggcc aacccagtga gcgatgcgat ccagatccag
     9121 gcgagtgcct tcttcttttt cgctagcgat tgggttgaag aaaccaacgc gctcgatgaa
     9181 gcgaccgttg cgtgcattac ggctgtcagc gacaacaacc tggtagaacg gacgcttttt
     9241 agcgccgtga cgtgctaaac gaatagttac cataacatcc tcttgtgtga ataaaacaac
     9301 cggaccccat cgaggaacgg agtccggtgt catattaaaa gcccgaaaat tttactgatt
     9361 tctggagaaa atgcaatcag cagttaataa ctctgtcgca taaggtcatt tagcgaccag
     9421 gaaatcccgg cggcatcatt cccttcatgc ttctcatcat cttcgccatt ccgcctttct
     9481 tcattttctt catcatgcgc tgcatgtcgt cgaactgctt cagaaggcgg ttaacgtcct
     9541 gtacctgcat cccgcaaccg gcggcgatac gacgcttacg cgaacctttg atgatttctg
     9601 gcttagcgcg ctctttcatc gtcattgagt tgatgatggc ttccatacgc accagcactt
     9661 tatcgtccat ctgcgacttg acgttatccg ggatctgccc catgcccggc agtttgccca
     9721 tcaagctggc catgccgccc atatttttca tctggcgcag ttgctcaaga aagtcgttga
     9781 gatcgaagcc gtcgcctttt tttagcttgc tggctaattt ctctgcctgc gcgcggtcaa
     9841 ctttgctttc gatatcttcg atcagcgaca gtacgtcgcc catgccgaga atacgcgacg
     9901 cgatgcggtc cggatggaac ggctccagcg cctcagtttt ctcgccaaca ccgaggaact
     9961 tgatcggttt gccagtgatg tgacgaatag agagcgccgc accgccgcgg gcatcgccgt
    10021 ccactttggt caacactacg ccggtaagcg gtaacgcttc attgaatgct tttgccgtat
    10081 tggccgcatc ctgaccggtc atggcgtcaa ccacaaacag ggtttcaacc gggttaatcg
    10141 acgcatggac ttgtttgatc tcgtccatca tcgcttcgtc aacgtgcaga cgaccagcgg
    10201 tatccaccag cagcacgtcg tagaatttca gtttggcttc tttcagcgcc gcgttaacaa
    10261 tatcaaccgg cttctgacca acatcagaag ggaagaaatc aacgcccacc tgctctgcca
    10321 gcgtctcaag ctgtttgatt gccgccgggc gatagacgtc agcagaaacc accagcactt
    10381 tcttcttgtg cttctcgcgc aggaaattac cgagtttacc aacgctggtt gttttaccgg
    10441 caccttgcag gcctgccatc agcacgaccg caggcggttg cgcagccaga ttcagggtct
    10501 ggttctcttc gcccatcgcc gcaaccagtt cgttacggac gattttgacg aactcctgcc
    10561 ccggcgtcag gctcttatta acttcatgac caaccgcttt ctcttttacg cgattgataa
    10621 actcacgcac taccggcaga gcgacgtccg cctccagcag cgccatgcgc acttcgcgca
    10681 gcgtatcttt tacgttgtct tcagtgaggc gtccacggcc actgatattg cgcagcgtgc
    10741 gcgacaaacg atcggttaaa ttatcaaaca ttgtctctcg cctggggtgg aaacggttgg
    10801 ccgcaatcgc gacacatcat cagtattttg ccgcagtata acatgaaggc gtctttgttg
    10861 ttatgcaacg gttggagcag cgttcacctg acgctatact gcttctcttt cttattgctc
    10921 aaactgtcga cattactatg cccgtttttg ctctgctcgc gcttgtcgcc tactccgtca
    10981 gtcttgcgct gattgttccc ggtctgctgc aaaaaaacgg cggctggcgg cgcatggcta
    11041 ttatttctgc ggtcattgcg ctggtctgcc acgcaatcgc tctggaagcc cgcatcctgc
    11101 ccgacggtga tagcggacaa aacctcagcc tgctgaacgt tggttcattg gtcagtttgg
    11161 tgatctgtac ggtaatgacc attgtggctt ctcgcaatcg tggctggttg ctgctaccca
    11221 ttgtctatgc ctttgcgctt atcaacctgg cgctggcgac cttcatgccc aatgaataca
    11281 tcacccatct ggaagcaacg cctgggatgc tggtgcacat tggcttatcg ctcttttcct
    11341 atgccacgct aattatcgcc gccctgtacg cgctgcaact ggcgtggatt gattaccaac
    11401 tgaagaacaa gaagctggca tttaaccagg aaatgccgcc attgatgagt atcgagcgta
    11461 aaatgttcca catcacgcag attggcgtgg tgctgctaac gctcacgctt tgcactggcc
    11521 tgttctacat gcacaaccta tttagcatgg aaaatatcga caaggctgtg ctctctatcg
    11581 tggcgtggtt tgtctatatt gtgctgctgt ggggacatta ccatgaagga tggcgtggac
    11641 gccgcgtcgt ctggtttaac gttgcgggcg cggtaattct gacactggcc tacttcggca
    11701 gccgaattgt ccagcagtta atcagctaaa cccagaaaag gagtttcccc tggaacacat
    11761 ttctactact acgttgatca ttattctgat catcatggtg gtcatttcag cctatttttc
    11821 cgggtccgaa accggaatga tgaccctcaa ccgctatcgt cttcgacata tggcgaaaca
    11881 gggtaatcgc tcggccaaac gcgtcgaaaa attgctgcgt aagccagacc gcctgataag
    11941 cctggtgtta atcggcaata acctggtcaa tattcttgcc tccgcgctcg gcactattgt
    12001 cgggatgcgt ttgtacggcg atgcgggcgt ggcaattgcg actggtgtgc tgacttttgt
    12061 cgtactggtt tttgctgagg tattgccgaa aaccattgcc gcgctgtacc cggaaaaagt
    12121 cgcttatccg agtagttttc tgctggctcc gctgcaaatt ttgatgatgc cgctggtctg
    12181 gttgctgaat gctatcaccc gtatgctgat gcgcatgatg ggtatcaaaa ccgatatcgt
    12241 ggttagcggc tctttgagca aagaagagtt gcgcactatc gtgcatgaat cgcgctcaca
    12301 aatttcccgt cgcaatcagg atatgctgct gtcggtgctc gatctggaaa aaatgaccgt
    12361 tgatgacatc atggtgccgc gcagtgaaat tatcggtatt gatatcaacg atgactggaa
    12421 atctattctg cgccaactct cccactcacc tcacgggcgc atcgtgcttt accgtgattc
    12481 gctggacgac gccatcagta tgctgcgagt acgtgaagcc tggcggttga tgtcggagaa
    12541 aaaagagttc accaaagaaa ccatgctgcg cgccgcggac gagatctatt ttgtgcctga
    12601 aggtacgccg ctcagcacgc agttggtaaa gtttcagcgc aacaaaaaga aagtcggcct
    12661 ggtcgtcaac gagtatggag acattcaggg gctggtgacg gttgaagata ttctggaaga
    12721 gattgtcggc gatttcacta cgtcgatgtc gccaacactt gccgaagagg tcacgccgca
    12781 aaacgacggt tcggtgatta tcgatggcac cgccaacgtg cgggaaatca acaaagcctt
    12841 taactggcat ctaccggaag atgatgcccg cacggttaat ggcgtcattc ttgaggcact
    12901 ggaagagatc cctgtcgcag gcacccgcgt gcgtattggc gagtacgata tcgatattct
    12961 cgacgtacag gacaatatga ttaagcaggt aaaagttttt cctgtgaaac cgctgcgcga
    13021 gagtgtggcg gagtaacgaa aacggccctg cattcgcatg cagggccgtg aattattacg
    13081 aaaacagaaa ttacgctttt gctttcgcta cagttaccat cgccgcacga atcgtacgac
    13141 cattcagcgt ataacccttc tgcataatgc ccagtacgtt acctggcgca acgtcatcag
    13201 attccaccat tgcgatagcc tgatgcacat tcgggtccag cggcacgtta gtttcggcga
    13261 tcacttcaac accaaactta cgcacaacat ccagcatcga cttcagcgtc agctcaatgc
    13321 cttcaaccat cgcagacata tccgggttag ctttatcagc cacttccagc gcacgatcca
    13381 ggctatcaat caccggcagc aattcgttga tgaatttctc cagcgcgaat ttgtgggctt
    13441 tttcaatatc cagttcagta cgacgacgca ggttttccat ttcggctttt acgcgcaaaa
    13501 taccgtcacg ttcacgggtc tgggcttcag ccagctgagc ttcgagattc gcaatttttt
    13561 catcgcgcgg atccacctgc tcagcagaag cttctggctc aactgcctca atctcttcgt
    13621 gctgatccat gataatttct tccggggctt gcccctcagg cgttttctgt tctttactac
    13681 tcatgaattt ctccgcgttt ttttcgcatt catctcgcta acttcgctta ttatggggat
    13741 cagtttcagg gtttcaaggg aagcactcac attgtcatca atcttcgcaa caaggacctc
    13801 ggaaaaatga ataatcattt caagtgcatt ggcattgtgg gacacccacg gcaccctact
    13861 gcactgacaa cacatgaaat gctctaccgc tggctgtgca caaaaggtta cgaggtcatc
    13921 gttgagcaac aaatcgctca cgaactgcaa ctgaagaatg tgaaaactgg cacgctcgcg
    13981 gagattgggc aactggctga tctcgcggta gtcgttggtg gcgacggtaa tatgctgggc
    14041 gcggcgcgca cgctcgcccg ttacgatatt aaagttattg gaatcaaccg tggcaacctg
    14101 ggtttcctga ctgaccttga ccccgataac gcccagcaac aattagccga tgtgctggaa
    14161 ggtcactaca tcagcgagaa acgttttttg ctggaagcgc aagtctgtca gcaagattgc
    14221 caaaaacgca tcagcaccgc gattaacgaa gtggtacttc atcctggcaa agtggcgcat
    14281 atgatagagt tcgaagtgta tatcgacgag atctttgcgt tttctcagcg atctgatggg
    14341 ctaattattt cgacgccaac aggctccacc gcctattccc tctctgcggg cggtcctatt
    14401 ctgaccccct ctctggatgc gattaccctg gtgcccatgt tcccgcatac gttgtcagca
    14461 cgaccactgg tcataaacag cagcagcacg atccgtctgc gtttttcgca tcgccgtaac
    14521 gacctggaaa tcagttgcga cagccagata gcactgccga ttcaggaagg tgaagatgtc
    14581 ctgattcgtc gctgtgatta ccatctgaat ctgattcatc cgaaagatta cagttatttc
    14641 aatacattaa gcaccaagct cggctggtca aaaaaattat tctaatttta cgccagcctc
    14701 tttactgtat ataaaaccag tttatactgt acacaataac agtaatggtt tttcatacag
    14761 gaaaacgact atgttggcac aactgaccat cagcaacttt gctatcgttc gtgagcttga
    14821 gattgatttt catagcggca tgaccgtaat aactggcgag accggtgcgg gtaaatctat
    14881 tgcaatagat gccctcggtc tttgtctcgg tggtcgcgct gaagccgaca tggtgcgtac
    14941 cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg aaagatacgc cagcggccct
    15001 gcgctggctg gaagaaaacc agcttgaaga cgggcatgaa tgtttgcttc gtcgcgtgat
    15061 cagcagtgat ggtcgctccc gtggtttcat caacggtaca gctgttcctc tgtcacaact
    15121 gcgcgaactg ggtcagttgc tgattcagat ccatggtcag cacgctcatc aattactcac
    15181 caaacctgag caccaaaaat tcctgcttga tggctatgcc aatgaaacct ctctacttca
    15241 ggaaatgacc gcacgttatc agttgtggca tcaaagctgc cgtgacctcg cgcatcatca
    15301 acagttaagt caggaacgcg ccgcccgtgc ggaactgctg caataccaat taaaagaact
    15361 taacgaattt aatccgcagc ccggagagtt tgagcaaatc gacgaagagt acaaacgtct
    15421 ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca ttggcattaa tggccgacgg
    15481 tgaagacgca aacctgcaaa gtcagcttta cacggctaaa caactggtga gcgaattgat
    15541 tggcatggac agcaaactgt ccggcgtact tgatatgctg gaagaagcta ccatccagat
    15601 tgctgaagcc agcgacgaac tgcgccacta ctgcgatcgt ctggatctcg atcccaaccg
    15661 actgtttgaa cttgaacagc gcatctcaaa acagatttcg ctggcacgta aacatcacgt
    15721 cagccctgag gcattaccac agtattacca gtcgctactg gaagaacagc agcaactgga
    15781 cgatcaggcc gactcacaag aaacgctcgc gctggcggta acgaaacatc atcagcaggc
    15841 actggaaacc gcgcgcgcat tacaccaaca acgccagcaa tatgcagaag aacttgcaca
    15901 gctgatcacc gacagtatgc atgcgctctc aatgccgcat gggcagttta cgattgatgt
    15961 caaatatgac gagcatcacc tgggcgctga cggtgccgat cgtattgagt ttcgggtaac
    16021 caccaaccca ggtcagccaa tgcagcctat tgccaaagtc gcatccggtg gtgaattgtc
    16081 ccgcatcgca ctggcaatcc aggtcatcac ggcgcgtaaa atggaaaccc cggcactgat
    16141 ttttgatgaa gtggatgtag ggattagcgg tccaacagcg gcagttgtcg gcaaactgct
    16201 gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc cacctgccac aagtcgcggg
    16261 atgtggtcat caacactatt ttgtcagcaa agaaaccgat ggtgcgatga cagaaacaca
    16321 tatgcaatct ctggataaaa aagcgcggtt acaagagctg gcgcgcctgc ttggtggcag
    16381 tgaagtcaca cgtaacacac tggcgaatgc gaaagaactg cttgcagcgt aaactttttt
    16441 cctgcttcac ggtcagagta aacagcaaaa cgccgtaaga ccggaaagca aaaggtttta
    16501 aagtgatgaa aggtctatta tcatcggcat attacagatg agccacgtac tgctcgggcc
    16561 cgaaaaggaa tcaaatcact atgcgctgta aaacgctgac tgctgcagca gcagtactat
    16621 tgatgttgac cgcaggctgt tccactctgg agcgagtggt ttaccgtcct gacatcaacc
    16681 aggggaacta tctgaccgct aacgacgtat ccaaaatacg tgttggcatg acgcaacaac
    16741 aagttgcgta cgcattgggt acaccgctga tgtccgatcc atttggtacg aatacctggt
    16801 tctatgtctt ccgccagcaa ccaggtcatg aaggtgtaac gcagcaaacg ctgacgctga
    16861 cctttaacag tagcggtgtg ttgaccaata ttgataacaa acctgcgctg agtggtaact
    16921 aataataaag ttgctctcaa agacgttaaa aaaggtgctc aatgagcacc ttttttctgt
    16981 ctgttattta ttcgctgatt tttctgctcg ttgcctgcga agctctttcg gatcggcaat
    17041 gagagggcga taaatctcca cccgatcgcc atcatgcaca ctatcgctta gttttgccgg
    17101 acggctgtaa atgccgactt tgtttttagt taaatcgata tcggtacgca attccagcaa
    17161 gccactggcg cgaatagctt cttcaaccgt cgcgccctcc tgcagcgtca ctcgctgcag
    17221 gtactgcttc tcaggtagcg cataagccac ctcaacggca attttaccta gcactgtaaa
    17281 cctctttcgc acgaaccgta aaagcctgga ccatattagc cgccagctct ttaaacacgc
    17341 gaccaaaggc gagttcaatc aacttattgg taaactcaaa gtcgagatga aactcgatac
    17401 gacacgcctc ctggctcagc ggcgtaaact tccatccacc aatcaatttc ttgaacggcc
    17461 catccaccag attcataaga atactttggt tactggtcaa ctggttgcgg gtagtaaacg
    17521 ttttgctgat cccagcctta gagacatcta ccgccgcagt catctgccca ggagtggact
    17581 ccagaatccg acttccggta caacctggca aaaactgagg ataagactga acgtcattca
    17641 ctaactgata catttgctcc gcgctgtagg gtaccagtgc ggtccggcta atctgaggca
    17701 taacaatttc catcaacaaa aatccaacaa ataatatcat ttatcctgct gtaaaaaaaa
    17761 cgctatcccg gcgctgggta acatcgggtt catgctaaga tagagccttg tcccccgcag
    17821 gattgatatg gggtgttttc gatttcagat taccgatgat tcacgacgct tatgacgaag
    17881 aaaaaagcac ataaacctgg ttcagcgacc atcgcgctta acaagcgcgc ccgtcacgaa
    17941 tactttatcg aagaagagtt cgaagcggga cttgccctgc aaggctggga agttaaatcc
    18001 ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc ttctgcgtga cggagaggca
    18061 tttctgtttg gcgctaacat cacgccaatg gccgtggcct ccacgcatgt ggtgtgcgat
    18121 cctacccgta cccgcaagtt acttctcaac cagcgcgaac tggactcatt gtacggtcgc
    18181 gtcaatcgag aaggctatac cgtagtggcg ctctccctgt actggaaaaa tgcctggtgc
    18241 aaagtgaaaa tcggcgtcgc caaaggtaaa aaacagcacg ataaacgttc agatatcaaa
    18301 gaacgcgaat ggcaggtgga taaagcacgc atcatgaaaa acgcccaccg ttaaacctgc
    18361 actccaatta ttgaccagtt cctcaccgcg cctccctctc cggcggcgcg aatgaacatc
    18421 ttattggcta tcacatccga cacaaatgtt gccatcccat tgcttaatcg aataaaaatc
    18481 aggctacatg ggtgctaaat ctttaacgat aacgccattg aggctggtca tggcgctcat
    18541 aaatctggta tacttacctt tacacattgg ggctgattct ggattcgacg ggatttgcga
    18601 aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa agccgcaaaa aatagtcgca
    18661 aacgacgaaa actacgcttt agcagcttaa taacctgctt agagccctct ctccctagcc
    18721 tccgctctta ggacggggat caagagaggt caaacccaaa agagatcgcg tggaagccct
    18781 gcctggggtt gaagcgttaa aacttaatca ggctagtttg ttagtggcgt gtccgtccgc
    18841 agctggcaag cgaatgtaaa gactgactaa gcatgtagta ccgaggatgt aggaatttcg
    18901 gacgcgggtt caactcccgc cagctccacc aaaattctcc atcggtgatt accagagtca
    18961 tccgatgaag tcctaagagc ccgcacggcg caagccctgc gggctttttt gtgccccgaa
    19021 tttgtcccgc gaagtctgat gccaactaat taaatccgat ccttttaggc accttgttag
    19081 gcacttcata aagctatatt gtctttgagg tgcctaaaac tatggaaacc cggcaatggc
    19141 aagacaaacc aaacctctat ccgttaagga aatcgaatct gctaaaccca aggaagcgga
    19201 ctacgttctc tatgatgacg atggccttga gctactcatt aaatccagcg ggagtaaaat
    19261 ctggcagttt cgctacattc gccctgtcac caagacacga gcaaagaaaa gcataggccc
    19321 ttacccgtca gttacccttg ccgatgccag aaactaccgt gcagagtccc gctcactcct
    19381 agctaaacag attgagccgc aggaacatca gcaagaacaa ttgcgtagtt cgctggaagc
    19441 caaaaccaat actttccagc tcgtagctga acgttggtgg aatgtgaaga aagccagtgt
    19501 gacagaggac tatgcggaag atatctggcg ctctcttgaa agagatgtct ttcctgcgat
    19561 tggcgacgtt agcgttacag atattaaagc tcatacactg gttcaggccg tacaaccggt
    19621 tcaggccaga ggagcactgg aaacggttcg tcgccagtgt caacgtatta atgaggtcat
    19681 gatctatgcc cagaacacag ggctgattga tgctgttccc agcgttaata tcggtaaagc
    19741 cttcgaaaag cctcagaaaa agaacatgcc cagcattcga ccggatcagc tatctcaact
    19801 gatgcagaca atgcgaacag ccagcattag cctttccaca cgctgcctgt tcatgtggca
    19861 acttcttact attacccgcc ctgccgaagc ggctgaagct cgctgggaag aagtagacat
    19921 agaagcgcaa gagtggaaga ttcccgcagc acgcatgaaa atgaaccgcg accatactgt
    19981 tccattgtca gatgaagcaa tagctgtgct ggagatgatg aaaccgctaa gcggcaaccg
    20041 agaatttatc tttcctagcc gtatcaagcc caaccagcct atgaacagtc agactgttaa
    20101 cgcctcacta aaacgcgcag gttttggtgg tgtgcttgtt tcgcatggtc tgagatcaat
    20161 tgccagtacg gctcttaatg aacaaggctt tccgcctgat gccattgagg cagcattggc
    20221 ccatgtggat aagaatgagg ttcgtcgtgc ttataaccgc agcgattacc tggaacagcg
    20281 tcgcccgatg atgcaatggt gggcaaattt tgtgatggca gcggatcgtg gaagcatgat
    20341 tgaaggtggg ataaaaggaa tgaagctagt tgggtgacta aatagctgcg cggcatagtg
    20401 tcccactttc agccagccct tgcaggttaa gccaccagac agtaaacggc aggtgtcgcg
    20461 ttattaggcg cagctattta gtagtaatat taggattggt gattctgcac tccaga
//
LOCUS       DAJTYF010000070        19902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTYF010000070 DAJTYF010000000
VERSION     DAJTYF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.070"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..374
                     /locus_tag="OO590_002574"
     CDS             <1..374
                     /locus_tag="OO590_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HCR6637894.1"
                     /translation="AMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSE
                     RPIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIGF
                     FGILAAIAITLVWQRHTRFFH"
     gene            complement(410..1366)
                     /locus_tag="OO590_002575"
     CDS             complement(410..1366)
                     /locus_tag="OO590_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR6637895.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1366..2607)
                     /gene="clsB"
                     /locus_tag="OO590_002576"
     CDS             complement(1366..2607)
                     /gene="clsB"
                     /locus_tag="OO590_002576"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6637896.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2604..3365)
                     /locus_tag="OO590_002577"
     CDS             complement(2604..3365)
                     /locus_tag="OO590_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6637897.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3498..3908
                     /locus_tag="OO590_002578"
     CDS             3498..3908
                     /locus_tag="OO590_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6637898.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3870..4976)
                     /locus_tag="OO590_002579"
     CDS             complement(3870..4976)
                     /locus_tag="OO590_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6637899.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4987..6120)
                     /locus_tag="OO590_002580"
     CDS             complement(4987..6120)
                     /locus_tag="OO590_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6637900.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6113..7849)
                     /locus_tag="OO590_002581"
     CDS             complement(6113..7849)
                     /locus_tag="OO590_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6637901.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7842..8837)
                     /gene="hlyD"
                     /locus_tag="OO590_002582"
     CDS             complement(7842..8837)
                     /gene="hlyD"
                     /locus_tag="OO590_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6637902.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8840..9511)
                     /gene="cecR"
                     /locus_tag="OO590_002583"
     CDS             complement(8840..9511)
                     /gene="cecR"
                     /locus_tag="OO590_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6637903.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9740..10927
                     /gene="rhlE"
                     /locus_tag="OO590_002584"
                     /pseudo
     CDS             9740..10927
                     /gene="rhlE"
                     /locus_tag="OO590_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(11134..11616)
                     /gene="ybiA"
                     /locus_tag="OO590_002585"
     CDS             complement(11134..11616)
                     /gene="ybiA"
                     /locus_tag="OO590_002585"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6637904.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            11736..13886
                     /gene="dinG"
                     /locus_tag="OO590_002586"
     CDS             11736..13886
                     /gene="dinG"
                     /locus_tag="OO590_002586"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6637905.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13914..14876
                     /gene="ybiB"
                     /locus_tag="OO590_002587"
     CDS             13914..14876
                     /gene="ybiB"
                     /locus_tag="OO590_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6637906.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15017..16102
                     /gene="hcxB"
                     /locus_tag="OO590_002588"
     CDS             15017..16102
                     /gene="hcxB"
                     /locus_tag="OO590_002588"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6637907.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHKETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(16331..16591)
                     /locus_tag="OO590_002589"
     CDS             complement(16331..16591)
                     /locus_tag="OO590_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6637908.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16856..17122)
                     /gene="ybiI"
                     /locus_tag="OO590_002590"
     CDS             complement(16856..17122)
                     /gene="ybiI"
                     /locus_tag="OO590_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6637909.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17196..17873)
                     /gene="ybiX"
                     /locus_tag="OO590_002591"
     CDS             complement(17196..17873)
                     /gene="ybiX"
                     /locus_tag="OO590_002591"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6637910.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(17915..19902)
                     /locus_tag="OO590_002592"
                     /pseudo
     CDS             complement(17915..19902)
                     /locus_tag="OO590_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4613 a         5231 c         5375 g         4683 t
ORIGIN      
        1 gcgcgatgtt tttaatcagt atgctggccg gtttattatt taatgttgat cctggttctc
       61 accgttttat cattatgatg acgttgacag ggttggccct ggtaatcatc gtgaatgcgg
      121 cattaatgag tgaacggccc atttgggtaa taagttgctt aatgattgtg ttatggccag
      181 gcattatctc gcatggacga aataagctcc ttgaactggc aggaaaatgc catagtgaag
      241 agttgtggag tccgattcgt tgcgctttta caggtgcatt aacactctat tactatttta
      301 tcggcttctt tgggatactt gccgcgatag ctataacgct tgtctggcaa aggcatacgc
      361 gtttttttca ttagtcgtaa atgcagatac taaagaactt aactaaactt cacatcgccg
      421 cttcattttt cgcccgtagc ttcttcgctt ggctttccag tagcaggtaa cagatcagcg
      481 ccagcagcag cgggataaag taatacagga cgcgataagc gagcagggcg gcgataatgc
      541 tccctttcga ggtatgctcc ccagccagta gtgcgataaa caccgcttcc agcacaccga
      601 tccccgccgg aatatgcacg atgacgccag caatactact aaccagtaac acgcccagta
      661 caaagaaata gttcacgctt tgaccaagta acagccagat aatcgccccc attaccatcc
      721 agttaacgct ggaaatcagc atctgagcga gggcgaattt ccatgaaggc agcaccagct
      781 tttgcccttt gatggtcata tggcggtgct tcgcgaaagc gcaaaaccac aaataaaccg
      841 cgataatcat cagtaagcca atgccgagaa tacgcagcgt agtttgatcg acataccagt
      901 gatccggcaa ctccaccacg cctgcggtaa agataatccc tgccagtaaa atatagccca
      961 gccagttggt ggtaatactg agcgaggaaa tccgcgtaat agtgctgccc ggtaacccta
     1021 gtcgggagta caaacgatag cgcataccaa tgccgccgac ccaggtactg agcgtcaggt
     1081 tgaaggcgta gcagataaac gacaccagca tcacctggcg cttcgccagt ttgtgaccgc
     1141 agtaaaagcg ggcgagcagg tcatagcagc cgtaaatcag atagctgacg accaccagcc
     1201 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
     1261 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
     1321 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
     1381 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg ggggatcaac
     1441 ctgcgtcaga cgtggtgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
     1501 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
     1561 ttcatccacc tgctgacaat ctgcggcaat aatgccgttc agattatcgc gcagcgtctg
     1621 gttaaaatga cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
     1681 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcca ctttgccgtg
     1741 gagcgggcgg cggcggtact caaaaacctg aacgccgcct ttaaccagat agttatacag
     1801 caagcgcgca ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
     1861 ccgcaccccg cgccgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
     1921 gtaggcgttg gcgataatca cttcccgccg cgcctgagtg agcattttca gataatggcg
     1981 ttcaatatca tcgcgatgtt cttcgttatc gcgccagacc agcaatacct gcgcttctcc
     2041 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
     2101 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
     2161 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
     2221 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
     2281 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
     2341 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
     2401 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
     2461 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
     2521 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
     2581 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
     2641 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
     2701 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
     2761 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
     2821 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
     2881 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
     2941 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
     3001 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
     3061 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
     3121 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
     3181 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
     3241 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
     3301 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
     3361 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
     3421 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
     3481 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
     3541 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
     3601 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
     3661 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
     3721 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
     3781 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
     3841 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
     3901 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
     3961 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
     4021 ttaatccacg tcagattttg cagccacact ggcatgtttt ccaccggcga aacgtaaccg
     4081 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
     4141 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
     4201 gtaaagtaga acagcgctag cgatccggcg aaggggattt gatacgccca gataccaatc
     4261 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
     4321 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
     4381 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
     4441 agcgacggca ccacaaacca tttgtagtcg agattcgggt tataccagtt gcgtaccacc
     4501 agctcgctgt tgttgggttt cggttttcct tccagcagct cctgctgata atttttgacg
     4561 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
     4621 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
     4681 accagtagta acgccttttg tgtgtcgatg gtcgggcgga tctcctgtgg gcttttcagc
     4741 agcagcacat gtgtaaaggc gctggcgcgg gcaaaacgtt gagtaagttc caccgaatgc
     4801 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
     4861 aacgggaaca ggatcacctg aattagcacg ggtaaaatca gaatcgcgcg ggtttgtggt
     4921 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
     4981 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
     5041 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
     5101 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
     5161 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcagaaa
     5221 agcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
     5281 cagccccatt cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaatcagcag
     5341 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcaacatcgc
     5401 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
     5461 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
     5521 ggtgagaatc gcgccgatga ccgtcatgat aatggtcacc gcacccggga taataaagtg
     5581 ctggcttatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
     5641 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
     5701 cccctgcaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
     5761 ggtggcgttg gcgcgctcca tctgttcggc gaaatccacg ggaataacca ccagaccgcg
     5821 aattttcccc gactgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
     5881 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
     5941 cagcaaaatc ccgacccgca gcttgctgga gtcgaggtta atgccgtaac caaaaataaa
     6001 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
     6061 ccgcgtctct ttgacgcaca gcgcccgcac gcgatgccag gaaaggatcg ggttactcat
     6121 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
     6181 cgggttgctc atcgttagct gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
     6241 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
     6301 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatgtgcagc caaaattcac
     6361 ggcgggtgag ggggtcaacg ccggaagtcg gttcgtcgag aaagagaata tccggttcat
     6421 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
     6481 cgtgtgaggc aatacttttc aggccaaatg cctcgctcat acgggagatt ttttcgttct
     6541 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
     6601 ggttgccgta gagcgaaaat ttttgcgcca tatagccgag atgctggcgc gctttaccgg
     6661 aactctcttt cagatccatc cccagcacca gcgcctggcc ggaagtcggc accagcaaac
     6721 cgcacatcat cttaaaggtg gtcgatttac ccgcgccgtt tggccccagc aaaccaaaaa
     6781 tctccccacg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
     6841 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgcaata
     6901 ttgcacccag cggcgattct gaggttccgg caccgcccag caaatcaata aacgcatctt
     6961 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
     7021 cgtctggtgt ggcctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
     7081 cgctgacctg cggcagtttc aaggcgcgtt gcaacagttt gcggttgccc tcgtgtggac
     7141 tggtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tctccctgat
     7201 acagcaactc gccttcgttc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
     7261 acgaggtact ccagaggatt aacatccctt cgcccgccag ctcatgcacc atctgccaca
     7321 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
     7381 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
     7441 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
     7501 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
     7561 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
     7621 cgccgtcgtt tttgatcgga tcaaagccaa tcaccgtggc actgccgctg tcgggtttca
     7681 gtaatcccgc caacatccgc atcagcgtgg ttttacctgc accgtccggc cccaccaacc
     7741 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
     7801 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
     7861 tcaccgaatt gtaccgtcac tggcattccc tggcgtaacg catcatcggc gtcggtcacc
     7921 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
     7981 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
     8041 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagatt ccgttcatca
     8101 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
     8161 ttgagaaccg tgcctggctc taccgcgcgc gttaacagcg tgccatcaga cggtgctatc
     8221 aacgttgagt cctgtaaatt cagctccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
     8281 ctggctttcg cctgagcgat atcctgttca cggttaccgg aacggtactg acgcaattta
     8341 tcctgtgctg atttcagcgt tgcctgtgcc tggtcgcgcg aggagcgggc attttccagg
     8401 tcatttgccg aaatggtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
     8461 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
     8521 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
     8581 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
     8641 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
     8701 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ttggcggctt
     8761 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagtccgatc
     8821 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
     8881 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
     8941 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
     9001 catgggaatg aaggatcatg cgggtgtcat tagcgtcgca gccggtccag gcggcaatca
     9061 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
     9121 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
     9181 tgagcagctt aatcatgttc ctgcaggcgc gaaggatcag ttcacggatg gcagcccgat
     9241 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
     9301 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
     9361 agtaggtgat ggcagcgata ttctgcccgg cctgggcggc tatctcgcgg gtggtggcgt
     9421 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
     9481 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
     9541 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
     9601 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct ccctcatgac
     9661 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
     9721 ggggcggttc ggagtagtta tgtctttcga ttctttgggt ttaagccctg atatcctgcg
     9781 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
     9841 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
     9901 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
     9961 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcacaga ttggcgaaaa
    10021 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gcggtgtcag
    10081 cattaaccca caaatgatga agttgcgcgg cggcgttgat gtgctggtgg caacgccggg
    10141 acgtttgctg gacctggaac atcaaaatgc agtgaagctg gatcaggttg aaatcctcgt
    10201 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    10261 aacaaaactg ccagcgaaac gccagaacct gctgttctct gcgaccttct ctgacgatat
    10321 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    10381 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    10441 attgctgtcg cacatgattg gtaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    10501 caaacacggc gctaaccatc tggctgagca gctcaataaa gatggcatcc gtagtgtggc
    10561 gatccacggg aataaatcgc aaggtgcgcg tacccgtgcg ctggctgatt ttaaatcggg
    10621 tgacattcgc gtactggtgg cgaccgatat cgctgcgcgc ggtctggata tcgaagagtt
    10681 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc accgtatcgg
    10741 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    10801 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    10861 gggctatgag ccggatccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    10921 tggcggcatc aggctgatga acaaacgcaa aactgcctga tgcgctacgc ttatcaggcc
    10981 tacgtggact ctgcaatata ttgaatttgc atgcttttgt aggccggata aggcgtttac
    11041 gccgcatccg gcatttcaca acaagcactt gtcagcaatt tgagaacacg ggaaaaaatt
    11101 tatctgtttt accgcccata agccaccgag acgttacttc tctatagcca attgttcgcg
    11161 caactccatt aaaaggtagc ccagtcgatt cttgccctta ccattaccac cgtctcccca
    11221 gtaagcatcg ttttccgtat gctcaaccag ttttgcgggc gcggttgcca gcaagagcac
    11281 acgcagttct gcatgctgtt cgaatttagc gcgaagtgct ttacgcatca cctgttcttt
    11341 aaccgactcc cagtttttac gaagtggctt ggaacgatta cgccccatgc gagcggcgac
    11401 catgggggaa gaaacccgac ggatctcttc gcggtatttt tcatcaagga atttttgtgc
    11461 ctgaaaatag tgttcggagg tgggccaggt attcccgtca accttgatgg gcctggcggc
    11521 aaagttagaa aaatcaccgt agtcatcgct ggtgctgtag aaatttatga tggtgtcttg
    11581 catgacgtgt tggatccttt gtgctcgaac gggcattaaa ccgcattatg ttggtggtta
    11641 ttgcgagccg ctttccagaa acagaaaaac cattacccct gaaaaccgaa aaatgccaca
    11701 atattggctg tttatacagt atttcaggtt ttctcatggc attaaccgcc gcgcttaaag
    11761 cgcaaattgc cgcctggtat aaggcgcttc aggaacagat ccccgacttt attccccgtg
    11821 cgccgcagcg gcagatgatt gcggacgtcg ccaaaacgct ggccggagaa gaagggcggc
    11881 atctggcgat tgaagccccc accggcgttg ggaaaacgct ctcctatttg atccccggca
    11941 tcgccattgc ccgcgaagag caaaaaacgc tggtggtgag taccgccaac gtggcgttgc
    12001 aggatcagat ttacagcaaa gatttaccgc tgctgaaaaa gatcattccc gatcttaaat
    12061 tcactgccgc tttcgggcgt gggcgctacg tttgtccgcg taatctgacg gcgctcgcca
    12121 gcactgaacc cacgcaacag gatctgctgg cgtttcttga cgacgaactg acgccaaata
    12181 atcaggaaga gcaaaaacgt tgtgcgaagc tgaagggcga tctcgacact tataaatggg
    12241 atggtctgcg cgatcatacg gatatcgcta tagatgatga tctctggcgt cgtttgagta
    12301 ccgacaaagc cagctgcctc aaccgtaact gttactacta tcgtgaatgc ccgttttttg
    12361 ttactcgtcg ggagattcag gaagcggaag tggtggtggc aaaccatgcg ctggtgatgg
    12421 cggcgatgga aagcgaagcc gtactgcctg acccgaaaaa tctgctgctg gtgctggacg
    12481 aaggtcatca cctgccagat gtggcccggg atgcgctgga gatgagcgct gaaatcaccg
    12541 cgccctggta tcgactacag ctggacttgt tcacgaaact ggtcgctacc tgcatggagc
    12601 agtttcgccc gaagaccatc ccaccgctgg cgatccctga acgtttgaat gcgcattgtg
    12661 aagagttgta tgagcttatc gcctcgttaa acaacattct caatctctac atgcctgccg
    12721 ggcaggaggc agagcaccgt tttgcgatgg gcgaactgcc tgatgaattg ctggagatct
    12781 gccagcggct ggcaaaactc accgagatgc tgcgtggtct ggcggagtta tttcttaacg
    12841 atttaagtga gaaaaccggc agccacgata ttgtgcgtct gcatcggttg attttacaga
    12901 tgaaccgcgc gctggggatg tttgaggtgc aaagcaaact ctggcggctg gcgtctctgg
    12961 cgcaatcttc cggtgcgccg gtgaccaaat gggcgacgcg ggaagagcgc gaagggcagc
    13021 tacacctctg gtttcactgc gtgggaatac gtgtcagcga tcagctggaa aggctgctgt
    13081 ggcgcagtat tccgcacatt attgtcacct ccgcaacctt gcgttcgctg aatagttttt
    13141 cgcgtttgca ggagatgagt ggtctgaaag agaaagcggg cgaccgtttt gtggcgctgg
    13201 attccccctt taaccactgc gaacagggca aaattgttat tccccggatg cgctttgagc
    13261 cttccatcga caacgaagag caacacattg ccgaaatggc ggcctttttc cgtgaacagg
    13321 tggagagcaa aaaacatctc ggtatgttgg tactgtttgc cagcggacgg gcgatgcagc
    13381 gctttctcga ctatgtgacg gatttacgcc tgatgttgct ggtgcagggc gatcagccgc
    13441 gttatcgctt agttgaactg caccgcaaac gcgttgccaa cggtgagcgt agcgtgctgg
    13501 tgggcttaca gtcatttgcc gaagggcttg atttgaaagg tgatctgctc agccaggtgc
    13561 atatccacaa aatcgctttt ccgcccatcg acagcccggt ggtgatcacc gaaggggaat
    13621 ggctgaaaag cctcaaccgc tatccgtttg aggtgcaaag cctgccgagc gcctcgttta
    13681 acctgattca gcaggttggg cgactgattc gaagccacgg ttgctggggc gaagtggtta
    13741 tctacgacaa acgcttgctg acaaaaaatt atggcaagcg actactggat gcattaccgg
    13801 tatttccgat agagcaaccg gaagtccctg aaggtatagt taaaaagaaa gaaaaaacga
    13861 aatccccacg ccgtcggcgg cgttaatgat gtgagtcagg taaggagtcg taaatggact
    13921 atcgcaaaat aattaaagag atcgggcgcg ggaaaaacca cgcgcgcgat ttagaccggg
    13981 atactgcgcg cggtctgtat gctcatatgc tcaatggtga agtccctgac cttgagttgg
    14041 gcggcgtatt gattgcgctg cgtatcaaag gggaagggga agcagagatg ctcggctttt
    14101 acgaagccat gcaaaatcac accatcaagc tgacgccgcc tgcaggcaag ccgatgccga
    14161 ttgtcatccc cagttacaac ggcgcgcgta aacaggctaa cctgacgcca ttattggcga
    14221 ttctgctgca taaactcggt tttcctgtgg tggttcacgg agttagcgaa gatccaactc
    14281 gcgtgctgac agaaaccatt ttcgaactga tgggtattac accaacgttg cacggcgggc
    14341 aggcgcaggc gaagctcgac gaacatcaac cggtgtttat gcctgtcggc gcgttttgcc
    14401 cgccgctgga aaaacaactg gcgatgcgct ggcggatggg cgtgcgtaac agtgcacata
    14461 ccctggcgaa actggcgacg ccatttgccg aaggtgaggc actgtgtctt tccagcgttt
    14521 cgcatccgga atacattgga cgcgtcgcga agttctttag cgatatcggc gggtgggcgt
    14581 tactaatgca tggtactgaa ggcgaagtgt atgctaatcc gcagcgctgc ccgcagatca
    14641 atctcattga ccgtgaaggg atgcgggtgc tgtatgaaaa acaggaccct gctggtagcg
    14701 agttactgcc acaagcaaaa gatccggaaa ccacggcgca gtggactgag cgttgcctgg
    14761 ctggcagcga agcgattccc gaatcgctga aaatccagat ggcttgctgc ctggtggcta
    14821 cgggtgaagc ggcaactatc agcgatggcc tggcgcgcgt taatcaggca ttttaatttt
    14881 tatctcagcc ggatgagcca cgtcatccgg cttttacccc accgcaatat gaaattcctg
    14941 catctttatt gaccttcccg cgcccggcgt gcagcataaa aaaacaacaa acacataaca
    15001 taaacaggag ttaaccatgg aaagtggtca tcgctttgat gctcagacgc tgcacagttt
    15061 tattcaggct gtatttcgtc agatgggtag cgaggaacaa gaagcgaaat tagttgccga
    15121 tcatttaatc gcggcaaacc tggcagggca tgattctcat ggtattggca tgatcccaag
    15181 ctatgtgcgt tcctggagtc aggggcatct gcaaattaac catcatgcca aaatcgttaa
    15241 agaggcgggg gcggcagtca cgctcgatgg cgatcgcgca ttcggtcagg tcgcggcaca
    15301 cgaagcgatg gcgctgggga ttgagaaagc gcatcagcac ggtattgctg ccgtggcgct
    15361 acataactcg catcatatcg gccgtatcgg ttactgggcg gagcagtgtt cagcggcggg
    15421 gtttgtctct atccactttg ttagcgtggt cggtattcca atggtcgcgc cgttccacgg
    15481 tcgcgacagc cgctttggca ccaatccctt ctgtgtggtt ttccctcgta aagataattt
    15541 cccgctgttg cttgattacg ccaccagcgc cattgcattt ggcaaaaccc gcgtcgcctg
    15601 gcataaaggc gtccccgtgc cgccaggttg cctgattgac gttaacggcg tgccgacgac
    15661 caatccggcg gtaatgcagg agtcgccgtt ggggtcgctg ttgacctttg ccgaacataa
    15721 aggctacgcc cttgcagcga tgtgtgaaat tcttggcggg gcgctttctg gcggtaaaac
    15781 gacgcataag gaaacgttgc aaaccagccc cgatgccatt cttaactgca tgaccactat
    15841 catcatcaac ccggaactgt tcggcgcgcc ggattgtaac gcgcagaccg aagcctttgc
    15901 cgagtgggtg aaagcctcgc cgcatgatga cgataagccg attttgctac cgggcgagtg
    15961 ggaagtgaac acgcgtcgcg aacggcagga gcaggggatt ccattggatg cgggaagctg
    16021 gcaggccatt tgtgatgcgg cgcggcagat tggtatgtcg gaagagacgt tgcaggcttt
    16081 ctgtcagcag ttagccagct aaaaaaaagc ccgtccagtg gcggacgggc aaacaagggt
    16141 aacataggat caatgagggt tagagcatat gcgtctgtcg gcaaacagac agggaaatac
    16201 ttgtgctgga cgtagcgtaa acgcctgatc cgtccaacgg ttcggatttt gtaggcctga
    16261 taagacgcgg tagcgtcgca tcaggcatct gttgccggat gcggcataaa cgccttatcc
    16321 gtcctacgaa ttacttataa attaccgcag taccgcttaa tttattgttg ttggtggcgg
    16381 aagtgatgct gtatccactg gcaccggctg ccgccgcttt ctcagccagt ttcgcttcca
    16441 gggcatcgag ggtggatgcg ccatcggcag aaaccacgcc gattttattc atgttctgtg
    16501 cctgggatgc cgttaccggt tccgccgcga acacgccaaa tgacagagtt gaaagagcca
    16561 tagcagcaac aacagtattg atagttttca taattaatct ctcgcaggtg atttttgtat
    16621 aagtgacgtt gtttcgtcga tgtgatgagt atcacgtttt tttacgagag ataaaatcga
    16681 agagaattga ccgccttgtt caaataaatt gattgatatc taacatactg ataaaccatt
    16741 tatttgttgt acttatggac ttgttttatg ccgcgtggca gtcacagcag cgttaaaggc
    16801 acggaatgcc acgcaaaata caaattatac tcgctatgca tctcgccagt tgtagttaac
    16861 gtaactggct gtctttcgaa cctctgcgat tatatcctgt atacgctggt ttttgtaaat
    16921 ctttctcctg ctgacaatga atacataagc gcacgccagg aatggcttcc cgacgggctt
    16981 gcgggatggg ggcaccgcac tcttcacatt catacaggct ttcgccacgc ggaatttcac
    17041 cccgagcgcg ggcaatcgca tcttcaattg tactgttgat ctgttcgttg acggcgtcat
    17101 cgttagccca agcggatgcc atatcgtcct ccccatatca atgcttgtac agttaagtgt
    17161 agctaatata gggacgaacc tgggcagttc aagcgtcaga tctccgacca ttcccgcagc
    17221 agattatgat aaagattgag cagcgacagg atctcttcat tttcaccata gcggcttttc
    17281 agcgactgaa tatttttgtc cagttcaaac agcatggcgc gctttttatc atcgcggatc
    17341 atcgactgga tccacataaa tgatgccact cgtacgccgc gggttacggg tgtcacgcaa
    17401 tgcaggctgc tggagggata caacaccaga tcacctgccg ggagttttac ccgatgttgt
    17461 ccaaaggtgt cattaacgac cagttcgccg ccgtcgtagc tttgtggatc gcttaaaaac
    17521 agcgtggcag aaaggtcagt gcgcatccag ccgttttgtg gatggctgcg tactgcgcca
    17581 tccacatgga aaccataggt ttcattgttc tgatagcgat taaacagcgg cgtggaaagg
    17641 gtacgcggca aggcagcggc aaagaacaat gcatgttggt taaccgcgtt cagcacctca
    17701 ttttgcagtg cggcgtataa cgcgctgcgg gtgtctactt gctgattgtt cttaacttgt
    17761 gcaccttgtg ctccggtggt gacgcgtcca tccacccatt cggcttgttc cagttgttcg
    17821 cgaaaatggg cgacgtcctg tggcgataac acgccgggaa tgtggtacat catacaagtt
    17881 ctccaaaaag tggggcctgc gccccacatc tgaatcagaa atgcatattg gctgtgagca
    17941 agaaggttct tggctcgccc gggtgataac ggtagccgct cttgttgatt gaggcgacgt
    18001 aatcggtatc aaacaggttg taaacgttta gctggaagtc gagattgcga ttaacccgat
    18061 accccagttt ggcatctgcg acccagtaac cttctgtaaa tgctggcgtt cccaccgcgc
    18121 cgtctgaacc tttatgtata ctgccgatat agcgtgcgcc cgcgccaaca gagatatcat
    18181 cggttgcctg atattggctc cataaggtga aggcgtgctc cggggtatac ggcagcgatg
    18241 aggaaccatc ctgggcaaca tctttgccgt ttttgatggt tgcttttttc tgggtatagc
    18301 cgccaatcac ctgccacgcg ggagtgatat tccctgccac ggatatctca tagccttcga
    18361 cgcgtttctt accgtattgc gagtaagtcc cgtcatcatt ttgctcaact tcattttcga
    18421 tatcagtacg gaacagcgcg gcggtgagca acaggcgttt atccagaacc tgccatttgg
    18481 tgccaatctc gctggtgttg gctttttgcg gtttaaaatc ggtgcggttg gcactgttac
    18541 cactgccaga ctgcgcaagg gcgaagttgt tgccgcccgg aggctgctgg gaaacggcat
    18601 agttaatata gacattgccg ttttccgtca ggtgatacag cgccccggct ttccagttca
    18661 ccagattgcc cgacttggcg gtgtcgacgg tggtgactgg agaacctttt gccacaccag
    18721 ctgggcaggt gatggcaccg cgtccgctgc cgccgcaggc ggtggcactg tcatattcag
    18781 tatgataatt atccagacgg atcccgccgt tcagctcaaa atcacgggtg atttgcagcg
    18841 tatcgaaggc gtaaattgcg aaggtatccg tctgaccatt ggcgtttgcg ccgttgcgcg
    18901 tcaggccgcc gggatgaatt ctgctgtcag gatgataaat atttaccgcc ggtaacgtca
    18961 ccggattaac gccatagtta gtctgcgtct cacgggtaaa ttccacgccg gtactgacat
    19021 catgaccgat agaaccggta tagaacgtcg aggtcaggtt ggtctggttg gtgagaattt
    19081 tattactcac atctttggta ttcgccgtgc gtgactaggt ccagctattc acatcgctgg
    19141 tgggctgagt aatattcgac gccccgccca taatcgccgt catcaggtaa tcctgcttta
    19201 cgcgcgacca acgggtagta ttgcgaatgg tggtgttatc gttgatgtcg tgctcaaaac
    19261 gcatggtggc ggtgtcggtg gtcgaatcgt cgtaatcgga atccgtgccg taaaagttat
    19321 gagtatcaac ttttccggaa tgattcaggg ccgccgctcc cgcagatggg gcagaatagc
    19381 ccggcaaacc gatggtcgga atgccgccgt ctggcgtgtt gtgctgagtg acatgcagat
    19441 aattaagata caaacgattc gctgtaccaa ggccaaaaga gacagaaggg gcgacgccgt
    19501 aacgctcatt tttgactttg tcgcgtccgg catcgtgggt tttttcgccc atcacattca
    19561 ggcgcaccgc ggtagtatca ccaatgacct gattgacgtc cagcgtgccg cggcggaacc
    19621 aggcgctgcc aatactggcg gaggcgtcaa tgccggaatc attgcgcggc tgcttgctga
    19681 tcatattgat cgagcctgtc ggtgcgctgc gcccgtagtc ggtgccggac ggccctttaa
    19741 tcacttcgac ctgctcggta ttgaaggtgt cgcgcgagac gctgccgata tcgcgaatac
    19801 catcaatata aatactgtta gaggtatcgg caccacgcat ataaatggcg tcgccagtgg
    19861 tggagttacc gttctcaccc gcaaaaaacg cacccacgcc gg
//
LOCUS       DAJTYF010000071        19744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTYF010000071 DAJTYF010000000
VERSION     DAJTYF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.071"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            10..276
                     /locus_tag="OO590_002593"
     CDS             10..276
                     /locus_tag="OO590_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6637911.1"
                     /translation="MNNPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVV
                     TLIDDNIREEILRPEWIAGETGMSVRSLYRMFADIRFGSRAIYS"
     gene            251..442
                     /locus_tag="OO590_002594"
     CDS             251..442
                     /locus_tag="OO590_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6637912.1"
                     /translation="MVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFS
                     TVFKQRFGMTPGEYRRKFR"
     gene            complement(614..943)
                     /locus_tag="OO590_002595"
     CDS             complement(614..943)
                     /locus_tag="OO590_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6637913.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(948..1133)
                     /locus_tag="OO590_002596"
     CDS             complement(948..1133)
                     /locus_tag="OO590_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6637914.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1130..3769)
                     /locus_tag="OO590_002597"
     CDS             complement(1130..3769)
                     /locus_tag="OO590_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6637915.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3977..4966
                     /gene="ldhA"
                     /locus_tag="OO590_002598"
     CDS             3977..4966
                     /gene="ldhA"
                     /locus_tag="OO590_002598"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6637916.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5077..5424
                     /gene="hslJ"
                     /locus_tag="OO590_002599"
     CDS             5077..5424
                     /gene="hslJ"
                     /locus_tag="OO590_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6637917.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEISFGEKMMISGSMCNR
                     FSGEGKLSNGELTAKGLAMTRMMCANPQLNELDNTISEMLKEGAQVDLTANQLTLATA
                     KQTLTYKLADLMN"
     gene            complement(5421..5687)
                     /locus_tag="OO590_002600"
     CDS             complement(5421..5687)
                     /locus_tag="OO590_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6637918.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5961..9485
                     /gene="nifJ"
                     /locus_tag="OO590_002601"
     CDS             5961..9485
                     /gene="nifJ"
                     /locus_tag="OO590_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6637919.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9852..10981
                     /gene="ompN"
                     /locus_tag="OO590_002602"
                     /pseudo
     CDS             9852..10981
                     /gene="ompN"
                     /locus_tag="OO590_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            11122..11556
                     /gene="uspF"
                     /locus_tag="OO590_002603"
     CDS             11122..11556
                     /gene="uspF"
                     /locus_tag="OO590_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6637920.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11732..12667
                     /gene="ttcA"
                     /locus_tag="OO590_002604"
     CDS             11732..12667
                     /gene="ttcA"
                     /locus_tag="OO590_002604"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6637921.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12796..14163)
                     /gene="dbpA"
                     /locus_tag="OO590_002605"
     CDS             complement(12796..14163)
                     /gene="dbpA"
                     /locus_tag="OO590_002605"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6637922.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTLALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14641..15624)
                     /gene="zntB"
                     /locus_tag="OO590_002606"
     CDS             complement(14641..15624)
                     /gene="zntB"
                     /locus_tag="OO590_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6637923.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15879..17111
                     /gene="dgcM"
                     /locus_tag="OO590_002607"
                     /pseudo
     CDS             15879..17111
                     /gene="dgcM"
                     /locus_tag="OO590_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(17132..17695)
                     /gene="smrA"
                     /locus_tag="OO590_002608"
     CDS             complement(17132..17695)
                     /gene="smrA"
                     /locus_tag="OO590_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR6637924.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17949..19403)
                     /locus_tag="OO590_002609"
     CDS             complement(17949..19403)
                     /locus_tag="OO590_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR6637925.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19400..>19744)
                     /locus_tag="OO590_002610"
     CDS             complement(19400..>19744)
                     /locus_tag="OO590_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6637926.1"
                     /translation="GSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRAD
                     ILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLR
                     PGLSVTVSVDER"
BASE COUNT         5195 a         5000 c         4651 g         4898 t
ORIGIN      
        1 caggagagca tgaataatcc ggcactttct gaaacagaaa gtgaagctgc gctacaggcg
       61 atggtgtgtc tgcttcgccc agtacttcat cagcgggaat ctgtccaacc tcgccgtgaa
      121 cgtcagtttc aaaaagtggt tacgttgata gacgataata ttcgcgaaga gatattacgc
      181 ccggagtgga tagccggaga gacaggtatg tcagtacgta gtttgtaccg aatgtttgcc
      241 gatataaggt ttggtagtcg cgcaatatat tcgtaaccgt cgtctcgatt tttgtgcaga
      301 tgcgattcgc catgccgcag atgatgaaaa actggcaggc atcggctttc attggggatt
      361 ttctgaccag agtcatttct caacggtatt taagcaacgc tttgggatga cgccaggcga
      421 gtatcgccgt aaattccgct aactaattgg cgttgcatgt actgcaacgc caattagtta
      481 aaacagatat ttgactatgc atttttcagg gcgaaaggca atttgacagg agtttttgaa
      541 tattcacctt gtttcaggaa aatagatcaa tcgctttctc ctgtagcgtt atcccgatat
      601 tgcggttttg ctattaaaac tttcgcaccc atttaccgtt tatcccttgt acatattccc
      661 ccggtttggc ccgagctacg agcttttgcc cggcgagttt cgcgatatca tctacagaca
      721 cattattttg ctttgcccgt tgttggtaac tcgctttacg ttcggcatta atttcggtta
      781 ctaatttctc tgtttcagca tccgttttca gcgcaaccag ataaccataa aatgtttcac
      841 ctacccgccc ctgagttctg gcttcatcca gagtcaatgc cattacattg ctgcttacca
      901 gcccaacaag aaacgcacaa agaagtaatg ttttcttcat catcgcctca gaaaagatcg
      961 ctacgagttt caagcagctc ttcgacatct ttgtctgcct tgatgatgat ctcatgctca
     1021 attttaacgt tcatattgat agtgatcggt tccttaggtg cagcgacttc aatgcgaggc
     1081 gtacagccaa ccagcataaa agatgacgtc aacgcagcca gtaaaatttt cattgttttt
     1141 cctcacactc ttttccttgc ggacagtcat ttcccggcag acgtgcgttc tgctccaacc
     1201 atgcctggag attatcgcca aagcgtaaac tgcgccacag cgtaaaaata ttctcttcat
     1261 gatggtaatt aaggtttacc gtaccgcttt taccatcaac gcgactggta cctgtaatgt
     1321 tggcctgcat ggttaataaa ccgagattat ctaagttaat ttttgtcgat gaccggctaa
     1381 tttccatata gcgcaaccag ttaattgctg aacccgcagt catattgtct ttcaccaccg
     1441 catctgcggt atctttatcc agccgcaagg tcattggccc gctatttgcc agccaaccat
     1501 ctttcactat ccatttttcg ttgtttagcc ataacggcaa tgcaccacta aatgccccgg
     1561 acatggcgaa ttgtttcgga ttgacggcgc taaccagttc gctggatgag agattattca
     1621 gccgcaacag cgccgggtca tgttgcggca tacgtaattg tttcatcagc acgttaccgc
     1681 ctaacacatc gacgctaaca tcagtcaaca gcaagggttc ttcttcagtc cacggataac
     1741 gcccttgcaa atcagccgta atatttttcg ctgtcaccag attaatcact tcggcaattc
     1801 gcaacgtaac ggggccgcga gtccccagat gccaggctcc atcggcaaaa cggaaaggca
     1861 ggacaaaatc gacaccgtta acctgattat ctggcatcca ggcactaccg cctttcaaca
     1921 cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc agaaaatgca acctgagcat
     1981 acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg caccagcggc tggaatacag
     2041 tcagtgattg ttttggccac caggcgttac cgcgcagacg aataccgtcc cagcgaccat
     2101 ttacccgaac cgggccaatc tcaccaacat gtaaatcgcc tttaaagagg aaataggtcg
     2161 gatcgcgccc atcgacgcta aattttaagg ttgatggcgg taacacactg ccgccagtga
     2221 acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa ggagggatgc tgtgcgtcac
     2281 gtacccagac gacgggcttg tcgagaatta atcgcggctt ttctaccgtc atcgtaccgt
     2341 attgtaactg gtcgaaaccg gtggagagat cggtcagcgt aatcgtgctg tcatgccact
     2401 caccttttcc tgcgacatcc caggtggcat tcatcggtgt aaaactccct tttccccagt
     2461 agcgccactg ccagcggcca gcgtcaggga gaaaatcatt cgccagccca tccatatgca
     2521 gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat ggcctgcaaa cgtccgtcaa
     2581 caccacgttg ggtgactttt acacccgcta aaggccagcg gatttcatcg atgtccagcg
     2641 aatcgatgac tcttcccttt gaacgaagta acgcgcctgg ctcaaaggtc agcgttgggt
     2701 cagacagact tccacttagc tgcgcaggta aacgggcata taaaatgagg tccgcctgtt
     2761 tcgcttcacc ggtcagctgc agaggcagtt gactgttatc catgcttaat tttcctgggc
     2821 caaaattaag caccgcgttg cccttacccg cttgcccctg ggtcagcaca ctcagtcgtc
     2881 cgctgaccag agcgttctca agccctgcct gccagttgtc aactttgaca cccagtcggc
     2941 cactcaaagg aaaacctgca tacagccagc tccagcgacc atcgcttacg gtcaattgtt
     3001 gacgagtaat ttgccacggc aaatcgagca acggatcgcc gttatcccgt gccagcacga
     3061 tcaattgccc gctattttcc tgccagtcca gctcggcatc caccagtgac ggttcctgcg
     3121 gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag tccatccggc acgagcggca
     3181 tagtaaattc ccccaccagt ttaaccggcg gctgattttc aaacgcgacg acatccagtt
     3241 cgctgactgt aagttgttgc cctttcagct ggccttgaaa tttaactttt tcgccctgat
     3301 aacgcagttg ctggatatca gaggttaatg cgagagagag ttttccctgc cattcctgcc
     3361 agggagaaaa aatcagttta tcgatattga tccaggtgtt aggcagcatg gactgccact
     3421 gcgcgagggt ttttggagca gctggcgatt gctccgtctg cggcaatttc gccagacaag
     3481 cagaatcaag ttctaccgtg ccgacgttca ataaccagcg gctgggatgt gaaaggctgg
     3541 cgttggtgat atgcgcaagc tgacaatctc ccaccagata acggagatcg ggaatgatta
     3601 aacctttacg cgtaatgcgt gggctttcat ctaatgcaat acgtgtcccg agcggtagcc
     3661 agatgtccgc cagcgtggga acccacagcc cgagcgtcat cagcagcgtc aacggcacaa
     3721 gaataatcag taataacagc gcgagaacgg ctttatattt acccagcatg ggtagttaat
     3781 atcctgattt cgcgaaaaat taagcattca atacgggtat tgtggcatgt ttaaccgttc
     3841 agttgaaggt tgcgcctaca ctaagcatag ttgttgatga atttttcaat atcgccatgg
     3901 ctttcaatta tatttgaaat tttgtaaaat atttttagca gcttaaatgt gattcaacat
     3961 cactggagaa agtcttatga aactcgccgt ttatagcaca aaacagtacg acaagaagta
     4021 cctgcaacag gtgaacgagt cctttggctt tgagctggaa ttttttgact ttctgctgac
     4081 ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta tgtattttcg taaacgatga
     4141 cggcagccgc ccggtgctgg aagagctgaa aaagcacggc gttaaatata tcgccctgcg
     4201 ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa gaactggggc tgaaagtagt
     4261 ccgtgttcca gcctatgatc cagaggccgt tgctgaacac gccatcggta tgatgatgac
     4321 gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt gacgctaact tctctctgga
     4381 aggtctgacc ggctttacta tgtatggcaa aacggcaggc gttatcggta ccggtaaaat
     4441 cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg cgtctgctgg cgttcgatcc
     4501 gtatccaagt gcagcggcgc tggaactcgg tgtggagtat gtcgatctgc caaccctgtt
     4561 ctctgaatca gacgttatct ctctgcactg cccgctgaca ccggaaaact accatctgtt
     4621 gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg atcgtcaata ccagtcgcgg
     4681 tgcattgatt gattctcagg cggcaattga agcgctgaaa aatcagaaaa ttggttcgtt
     4741 gggtatggac gtctatgaga acgaacgcga tctgttcttt gaagataaat ccaacgacgt
     4801 gatccaggat gacgtattcc gtcgcctgtc tgcctgccac aacgtgctgt ttaccgggca
     4861 ccaggcattc ctgacagcag aagctctgac cagtatttct cagactacgc tgcaaaactt
     4921 aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg gtttaatctt gccgctcccc
     4981 tgcaatccag gggagctgat tcagataatc cccaatgacc tttcattctc tattcttaaa
     5041 atagtcctga gtcagaaact gtaattgaga accacaatga agaaagtagc cgcgtttgtt
     5101 gcgctaagcc tgctgatggc gggatgtgta agtaatgaca aaattgctgt tacgccagaa
     5161 atcagctttg gtgaaaaaat gatgatttcc ggcagcatgt gtaaccgctt tagcggtgaa
     5221 ggcaaactgt ctaatggtga actgacagcc aaagggctgg caatgacccg tatgatgtgc
     5281 gctaacccgc agcttaatga actcgataac accattagcg aaatgctgaa agaaggtgca
     5341 caagtggatc tgaccgcgaa ccagttaacg ctggcgaccg cgaaacagac attaacttat
     5401 aagctggcgg atttaatgaa ttaatagctg ccacagctcc cggcggcaag tgactgttcg
     5461 ctacagcgtt tgccgttggg taatgcacac atcccaatcg ccgtaccatc cagttgacgg
     5521 gcaacagaaa gcgaaccgcc gatcattgca caatttgctt ctccactact ggacatcgac
     5581 gcttttaaac ttggcgctac gtgcgccgca gtcgcctgct gaacaggctc actactacac
     5641 gccgacaaca ataaagcggc acaccctacc caaaacgctg ctcgcatctc ttcttcctct
     5701 gatcttcaag ccaaacgaca ccgccataaa taataggcag cacagagggc ggcgtcgaga
     5761 gctgtcctgc gcgttgcccc gcatttttac ttttttatgg ctattttttt gccctctgtt
     5821 tgatcaaaac attcattact ctgatgtggg ggacacaaaa gcgaaaatgc agaagaaagc
     5881 catttgctaa aattgaagga ttactactgg gcgcgcagca atttcgtgcg cccctcattt
     5941 gcgcaatgta agggtgtcat atgattacta ttgacggtaa tggcgcggtt gcttcggtgg
     6001 cgtttcgcac cagtgaagtt atcgccatct accctattac ccccagttcc acgatggcag
     6061 aacaggctga tgcctgggcc ggaaacggct tgaagaacgt ttggggagac acaccacgcg
     6121 tggttgaaat gcagtcggaa gcgggtgcta tcgctaccgt gcatggcgct ttgcagacgg
     6181 gtgccctttc aacatcgttt acgtcatcgc agggtttgct gctgatgatc ccgacgctgt
     6241 acaaactggc aggcgaactg acgccatttg tcctgcatgt agcggcacgt accgttgcca
     6301 cacatgcact ttctattttt ggcgatcatt ccgacgttat ggcggtgcgc cagacgggtt
     6361 gcgcgatgtt gtgtgcggct aacgtccagg aagcgcaaga ctttgctctc atttcacaca
     6421 tcgcaacgct gaagagccgc gtgccattta ttcatttctt tgatggtttt cgcacgtccc
     6481 acgaaatcaa taaaattgtc ccgctggccg atgacactat tcttgatctc atgccgcagg
     6541 ccgaaattga tgctcatcgc gcccgggcgc tcaacccgga acatccggtg atccgcggta
     6601 cgtccgccaa tcctgacact tatttccagt ctcgcgaagc caccaacccc tggtacaacg
     6661 cggtctatga ccatgttgaa caggcgatga atgatttcgc tgccgcgaca ggtcgtcagt
     6721 atcagccgtt tgagtattac gggcatccgc aagcggaacg ggtgattatc ctgatgggct
     6781 ctgccattgg cacctgtgaa gaagtggttg atgaattgct aacccgtggc gaaaaagttg
     6841 gcgtgctgaa agttcgtctg taccgtccct tctccgccaa acacttgctg caagctctgc
     6901 cgggatccgt acgcagcgtg gcggtactgg acagaaccaa agaacccggt gcccaggcag
     6961 aaccgctcta tctggatgtg atgaccgcac tggcaggagc ctttaataat ggtgagcgcg
     7021 aaaccctgcc ccgtgtcatt ggtgggcgct atggtctttc atccaaagaa tttggcccgg
     7081 actgtgtact ggcggtattt gccgagctca acgcggctaa acctaaagca cgctttacgg
     7141 ttggtattta cgatgatgtg accaatctgt cactgccgtt gccggaaaac actctgccaa
     7201 actcggcgaa acttgaagcc ttgttttatg gccttggtag tgatggcagc gtttccgcaa
     7261 ccaaaaacaa tatcaagatt atcggtaatt ccacgccgtg gtacgcacag ggctattttg
     7321 tttacgactc caaaaaagcg ggcggcctga cggtttctca ccttcgcgtg agcgaacagc
     7381 cgattcgttc cgcttatctc atttcccagg ctgattttgt tggctgccac cagttgcagt
     7441 ttatcgataa atatcagatg gctgagcgtt taaaacctag cggcattttc ctgcttaata
     7501 cgccgtacag cgcagatgaa gtgtggtcgc gcttgccgca ggaagttcag gccgtgttaa
     7561 accagaaaaa agcgcgcttc tatgtgatta acgcggcgaa aatcgcccgc gaatgtggcc
     7621 tggcggcccg tattaatacc gtcatgcaga tggctttttt ccatctgacg caaattctgc
     7681 ctggcgatag cgccctcgca gaattgcagg gtgcgattgc caaaagttac agtagcaaag
     7741 gtcaggatct ggtggagcgc aactggcagg ctctggcgct ggcgcgtgaa tccgtagaag
     7801 aagttccgtt gcaaccggta aatccgcaca gcgccaatcg accgccagtg gtttccgatg
     7861 ccgcccctga tttcgtgaaa accgtaaccg cggcgatgct cgccgggctt ggtgatgccc
     7921 tccccgtttc ggcgctgccg ccagacggca cctggccgat gggcaccact cgctgggaaa
     7981 aacgcaatat cgccgaagag atccccatct ggaaagagga actctgtacc caatgtaacc
     8041 actgcgtcgc cgcttgccca cactcagcta ttcgcgcaaa agtggtaccg cctgaagcga
     8101 tggaaaacgc ccctgccagc ctgcattcgc tagatgtgaa atcgcgtgat atgcgcgggc
     8161 agaaatatgt cttgcaggtg gcaccggaag attgcaccgg ttgtaacctg tgcgtcgaag
     8221 tttgcccggc gaaagaccgt cagaatccag agattaaagc catcaatatg atgtctcgcc
     8281 aggaacatgt cgaagaagag aaaatcaatt acgatttctt cctcaacctg ccagaaatcg
     8341 accgtagcaa actggaacgt attgatattc gtacatcgca gctgattaca ccgctgtttg
     8401 aatattcagg tgcttgcccc ggttgtggcg agacgccgta tattaaattg ctgactcagc
     8461 tctatggtga ccggatgttg atcgctaacg ccaccggctg ttcttcaatt tatggcggta
     8521 acctgccctc tacaccgtat accaccgatg ccaacggtcg tgggccggca tgggcgaact
     8581 ctctatttga agataatgcc gaatttggcc ttggtttccg cctgacggtc gatcaacacc
     8641 gtgtccgcgt gctgcgtctg ctggatcaat ttgccgataa aatcccggcg gaattactga
     8701 cggcgttgaa atcagacgct acgccagagg ttcgtcgaga acaggtcgca gctttacgcc
     8761 agcaactcaa cgatgttgcc gaagcacatg aactgctgcg tgacgcagat gcactggtgg
     8821 aaaaatcaat ctggctgatt ggtggcgacg gctgggctta cgatatcggc tttggcggtc
     8881 tggatcatgt attgagtttg acggaaaacg tcaacattct ggtgctggat acgcaatgct
     8941 attccaacac cggtggtcag gcgtcgaaag cgacaccgct gggtgcagta actaagttcg
     9001 gcgagcacgg caaacgtaaa gcgcgtaaag atcttggcgt cagtatgatg atgtacggtc
     9061 atgtttatgt ggcgcagatt tctctcggcg cgcagctgaa ccagacggtg aaagcgattc
     9121 aggaagcgga agcgtatccg ggaccatcgc tgatcattgc ttatagcccg tgtgaagagc
     9181 atggttacga tctggctctc agccacgacc agatgcgcca actcactgct accggcttct
     9241 ggccgctata tcgctttgat ccgcgtcgtg ccgatgaagg caaactgccg ctggcgctgg
     9301 attcacgccc gccgtcagaa gcactggaag aaacgttact tcacgagcag cgtttccgtc
     9361 gtctgaattc gcagcagcca gaagtggcag aacagttatg gaaagatgct gcagctgatt
     9421 tgcaaaaacg ctatgacttc ctagcacaaa tggccggaaa agcggaaaaa agcaacaccg
     9481 attaaatgct ctggataagg attatccaat tctaaaaaaa aagcccggac gactgttcgg
     9541 gcttgtcttt ttatatgttg gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa
     9601 tctgtgacaa taaaggcata taacccgcgc agaataacgt atagaaaatt aaaagtataa
     9661 tttttatttt gtatgtatta tcttcgatac attcaccaga cttattatca tttcagagga
     9721 attatttgat taaggtttta cttaaggcgt aacaaatgat atttatcggc aaactgaact
     9781 tctcctttag gatgttttca ctccccgaaa tggggataaa aggcaaataa aataactaaa
     9841 ggatttattc aatgaaaaga aaagtactgg cacttttaat ccctgccctg ctcgccgcag
     9901 gtgctgcaca tgcagccgaa gtttataata aagacggcaa caaattagat ctgtatggca
     9961 aagttgatgg cctgcattat ttttctgata attcagcgaa agatggcgac cagagctatg
    10021 cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca acttactggc tacggtcaat
    10081 gggaatacaa tattcaggca aacaacactg aatcttcaaa aaaccagtca tggacccgtc
    10141 tggcatttgc cggactgaaa tttgcagatt acggttcttt cgattacgga cgtaattatg
    10201 gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc taaatttggc ggtgactctt
    10261 ataccaatgc agacaacttt atgactggtc gagccaatgg cgtcgcgact tatcgtaata
    10321 ctgatttctt cggtctggta aatggtctga acttcgcggt gcagtatcaa ggtaacaacg
    10381 aaggagccag taatggtcag gaaggcacca ataacgaacg cgatgttcgt catgaaaacg
    10441 gtgacggctg gggtctttcc acaacatatg atttaggcat gggctttagc gctggtgcgg
    10501 catacacctc ttctgaccgc accaatgacc aggttaacca tactgcggcg ggtggtgata
    10561 aagcagacgc gtggactgct gggctaaaat acgatgctaa caatatttac ctagcaacca
    10621 tgtattcaga aacgcgtaat atgaccccgt ttggcgacag cgattatgca gttgcgaaca
    10681 aaacccagaa ctttgaagtt actagtacca gtttgatttt ggtctgcgtc cggcagtctc
    10741 tttcctgatg tctaaaggcc gtgaccttca cgctgctggt ggtgcagaca acccggcagg
    10801 tgttgatgat aaagatctgg ttaaatacgc cgatgttggc gcgacttact atttcaataa
    10861 aaacatgtcc acctatgttg actataaaat caacctgttg gatgaagatg acagtttcta
    10921 cgctgccaac ggcatctcta ccgatgatat tgtcgcttta ggtctggttt atcagttcta
    10981 aatcctcctg cccgctgtta tggcgggctt tttctgctta ttcttcctcc tttgatctaa
    11041 attaaaaatg tgaactccgt cattacacaa aaagtgtcat ctggcgttac actttatgcg
    11101 gatactaaaa caggaggttt tatgaacaga acgattcttg tccctatcga tatttcagat
    11161 tcagaattaa ctcaacgcgt gattagccac gttgaggcag aggcaaagat tgatgatgca
    11221 gaggttcatt tcctgacggt aataccttca ctgccctact atgcctctct gggtttagcg
    11281 tattccgcag aattaccggc aatggatgac ctgaaagcgg aagccaaatc gcaactggaa
    11341 gagatcatta aaaaatttaa actgccaacc gacagagtgc atgtccatgt tgaggaaggc
    11401 tcgcccaaag accgcattct ggaattggcg aagaagatcc ccgctcatat gatcatcatt
    11461 gcttcccatc gaccggatat caccacttat ctgctcggtt ccaacgccgc agctgtagtg
    11521 cgtcacgcag agtgctccgt gctggttgtg cgctgacact aacgcccgca cattgctgcg
    11581 ggctttttga ttcatttcgc aaatgtgctg acattttccc ctctaatccg taccatacac
    11641 gccacagttt ttatatcaga tttcttatgc tgagcgttcc ggcatgacgc atactcttct
    11701 gatgccatat aacgaattga gtcgctttta aatgtcgcaa aatcaagaaa ttagtaagaa
    11761 agaacaatac aacctgaaca aattacaaaa acgtctgcgt cgtaacgtgg gcgaagccat
    11821 tgctgacttc aatatgattg aagaaggcga tcgcatcatg gtttgcctct ccgggggtaa
    11881 agacagctat accatgctgg agattctgcg caatttgcag caaagcgcgc caatcaattt
    11941 ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc ttcccggaac acgttctgcc
    12001 cgagtatctt gaaaagctgg gcgttgagta caagattgtt gaagagaaca cttacggtat
    12061 cgtgaaagag aagattccag agggcaaaac cacttgctca ctgtgttctc ggctacgtcg
    12121 cggtatcctt tatcgcaccg caacggaact ggggacgacg aagatcgcgt tgggtcacca
    12181 tcgtgacgat atcctgcaaa cgttgttctt aaatatgttc tacggcggca agatgaaagg
    12241 tatgcctccg aaactgatga gcgatgatgg caaacatatc gttattcgtc cactggccta
    12301 ctgccgcgag aaagatattc agcgatttgc cgatgcaaaa gcgttcccga ttattccgtg
    12361 caacctgtgc ggttcacagc ctaacctgca acgtcaggtg attgctgaca tgttgcgtga
    12421 ctgggataaa cgttatccag ggcgtatcga gacgatgttc agcgcgatgc agaatgtggt
    12481 gccgtcgcat ctgtgcgata ccaacctgtt cgatttcaaa ggcattaccc acggttctga
    12541 agtggttaac gggggtgatc tggcgtttga tcgcgaagag atcccactac aaccggcggg
    12601 ctggcagcca gaagaagatg aaaatcagtt ggatgagtta cggctgaatg tggttgaagt
    12661 gaaataacca ggatagcgcc cgatgcgcaa gcgtatcggg ctactcttat ggaggccgga
    12721 taagacgcgg ccagcgtcgc atccggcaat cccgaataag atgtttactc ttgcacccgg
    12781 caattcaaca tttcattatt ttaataaccg cacccggcac gtttttcctt taatcttccc
    12841 gccctgtaac tgtttccatg ctttatgagc aacagcctga cggaccgcga catagacatg
    12901 cgccggatgc acggcgattt tgccaatatc tgcgccatca agcccgatat ctcctgtcag
    12961 tgcacctaat acatcacccg ggcgcatttt ggcttttttc ccgccatcga tacacaacgt
    13021 tgccatttct gcttccagcg tcacaatgga actattagct ggcggcgttt gccagttaag
    13081 ttttatctgc aacatgtcag aaatgatatt ggcccgctgt gcttcttccg gagcacagaa
    13141 actgatcgcc agaccgctat ttcctgcacg agctgtacga ccgatgcgat gtacatgaac
    13201 ttcagggtcc cacgccagct caaagttcac caccagctca agcgatttaa tatccagacc
    13261 acgcgcagca acatcagtcg caaccagtac acgggcgcta ccgttagcaa aacgtaccag
    13321 ggtctgatcg cgttgctcca gatcgccgtg taacgacaat gcactttgct ctacttcatt
    13381 cagcgcgtcg cagacagcct ggcaatcttt tttggtattg caaaacacca cacaagagga
    13441 tggctgatgc aagcttaata accgttgcaa cagaggaatt ttgcctttac tggatgtctc
    13501 ataaaattgt tgttcaatgg gtggcaaagc atctgttgag tcaatttcaa tcgccaaagg
    13561 atcgcgttgc actcgtccgc tgattgcggc gatggcttcc ggccaggttg ccgaaaacag
    13621 aagcgtctgt cgagatgcag gcgcaaaacg gatgacatca tcaatggcat cgctaaatcc
    13681 catatccagc atgcggtcgg cctcatccat caccaaagta ttcagcgcat ccagtgatac
    13741 cgtgcctttt tgcaagtgat ccagcaaacg acccggcgtt gccacgataa tatgcggcgc
    13801 atgttgcaac gaatcacgct gcataccgaa cggttgacca ccgcacaacg tcaaaatttt
    13861 ggtatttggc agaaaacgcg ccagccgacg caattcacct gccacctgat ccgccagttc
    13921 acgcgtagga cacagcacta aagccagggt ttgaaatagc gacgcatcaa tttgctgtaa
    13981 caagccgagg ccaaaagccg ccgttttgcc gctgccggtt ttcgcctgca cgcgaacatc
    14041 ttttcctgca aggatcgccg gaagcgcggc ggcctgcacc ggcgtcatgg ttaaataacc
    14101 caactcatta aggttcgtga gttgggcggg aggcaaaaca ttcagggtag aaaaagcggt
    14161 cacaatctat tctcgtggtc atcgacgcaa agttagcagg cgcgtatcct cgcagatcta
    14221 cgctcacgat gcgacaattt aatcggttct tcatcgggtg gtgggtcagg catgggttgc
    14281 gggcgaggga tcggatcggg cactggaaca ggatcgccag gaatcggttc agggacagga
    14341 atttgcaaat aaataagtgt cgtcatattt ccctccggtc attgggtgga ctcttaaagg
    14401 gtagacgctg ataaataaca ggcaaaaaaa agccgactca tcaaagtcgg cgtcgtacga
    14461 atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga caatatggag cgcaacgccc
    14521 atcgcttgac gttgcattca cctgcaaggg agatattgcc ctgaatgggt agagagttta
    14581 ttgacttcgc tcaaactttg cggcgttttt gtatacagac agccgaaaaa attgcttttg
    14641 ttacaaccat ttactacggt gcaaccataa agcaacacca ccaataagaa caactaacag
    14701 aatacaaaaa attgaaaatc cgaattgcca cccgccgcca gggatcccac caaggttgac
    14761 gccaaataac ccggtcagaa aggtactggg taaaaagacc attgccatca acgacattgt
    14821 ataggtacga cgagctaaat tttcctgcat cacctgagcg atttcatccg ccatcacgcc
    14881 agtccgtgct atacaggcgt cgatttcgtc aaggccgcgc ccaaggcgat cggcaatatc
    14941 ctgcatccga cggcgttggt catcgctcat ccacggcaaa cgttcactgg caagacgagc
    15001 ataaacatca cgttgcggtg ccatatagcg acgcatcaca attaattgtt tgcgcagcag
    15061 agccaggaat ccacgcggtg gaatttgctg atcaaggaga ttatcttcaa ggtcgataat
    15121 tttatcgtgc agctgctcga taaattcact ggaatgatcg gtcaacgcat cgcacacatc
    15181 caccagccat cccccgcaat cggtcggacc cgtgccctct tccagatcgc tcaccacatc
    15241 gtccagcgcc agcactttgc gttgtcgggt cgaaacaatt aaccgcccgt ccatatatac
    15301 acgcatggcg accagttgat cggggcgttc atcggtgctg ccgtttatac agcgcaatgt
    15361 aatcagcgtg ccttcaccga aacggctgac tcggggccgt gtgctctcgc ccgccagcgc
    15421 atcacgtacg ttattgggaa gcagcggtgt tgtcgccagc cattgggcgc tatcatggtg
    15481 tacataatta aggtggagcc agcagggatg cgcttcatca atcacatctg tattttccag
    15541 cggtttaacg ccgcctctac catccagcat ccaggcaaat actgcatccg ggacattaac
    15601 gtccgatccc ttaatcgctt ccacagtgcc tccatcatca acgcattatt ttgtagtcta
    15661 gccttctggc cctgttacgc aacatctcat caccccatta ccctgaaatg attaataaaa
    15721 ttctgtctaa attgaataca aaaagcaaaa tgcttttccg tatacaaacc gtgtgaagtg
    15781 ttaaatagcg tctatcatta tcagaattat ctgatcatat gacgtggctt ttttgcgatc
    15841 ggatagcaac aaaaattgat aaaaataacg ggatctcaat gattacgcac aacttcaata
    15901 ccctggactt actcaccagt cctgtctgga ttgtttcgcc ctttgaggaa cagttaattt
    15961 atgccaatag cgcggcgcga ctgttgatgc aagacctcac gtttagtcag ctacgaaccg
    16021 gaccctattc cgtctcctca caaaaagaac tgccgaaata cctctccgat ctgcaaaacc
    16081 aacacgatat tatcgaaatc ctcactgttc agcgtaaaga agaggaaaca gcattaagct
    16141 gtcggcttgt tttgcgagag ctgacagaaa cagaaccggt gattattttc gaaggtatcg
    16201 aagcgccggc aacgctgggt ttaaaagcca gtcgctcggc aaattatcag cgcaaaaaac
    16261 aaggttttta tgcgcgcttt tttctgacta actctgcacc aatgttgttg attgacccgt
    16321 cacgagatgg acaaatcgtc gatgctaacc tcgccgcgct caatttctat ggttataacc
    16381 atgaaacgat gtgccagaaa catacctagg aaataaatat gcttgggcgt cgcgtcatgc
    16441 ctatcatgca tgaaatttcg catttacccg gcggtcataa ccctttgaat tttgttcata
    16501 aactggcgga tggttctact cgtcatgtgc agacctatgc cggaccgatt gaaatttatg
    16561 gcgacaagct catgttatgt attgtgcatg atattactga gcaaaaacgg ctggaggagc
    16621 agctggaaca tgctgctcac catgacgcga tgaccggatt actgaatcgg cgacagtttt
    16681 atcacattac ggaaccaggc caaatgcagc atctcgccat cgctcaggat tacagcttgt
    16741 tgctcatcga caccgatcgt tttaaacaca ttaacgatct ctatgggcat tctaaaggtg
    16801 atgaggtgtt atgcgccctc gcccgccccc tcgaaagttg cgctcgcaaa ggcgatttgg
    16861 tgtttcgttg gggaggcgaa gagtttgtct tattgctacc aagaacccca ctggataccg
    16921 cgctttcgct ggctgaaact atccgcgtaa gcgtggcaaa agtgagtatt tcgggcttac
    16981 cacgctttac cgtcagcatt ggtgtggcgc atcacgaagg aaatgaaagc atcgatgaac
    17041 tgtttaaacg cgttgatgat gctttgtatc gggcgaaaaa tgatggacgc aaccgcgtgc
    17101 tggcggcata agccgcggat gcgtctcgag atcaacgact gcgcttagcg tggcgctccc
    17161 agttttcttg cttcgcctgc gccgttttac gtagtgcgac gtaacacgcc ccgctgccgc
    17221 catgatgcgg tagcgcggtg caatatgcct gaacatcatc aaattcggtc agccagcgcg
    17281 ccacatagct gcggacaata ttggcatgtg atttatcttc ccgcccttta ccatgaataa
    17341 tcagcacatt acgcaaacca tccgccaggg cttgttgaat gaaactgaac accattttgc
    17401 ggcactcttc caccggctgg cgcaaaagat tcaggctcgc ctgttgcgga tatttaccac
    17461 tgcgcagctt atccagcacc ccatgctgca acccttcctg ccgaaactcc agcggctgac
    17521 ttagcgggat gatgtcgaga aatccggtag tgaggaaatt atcaagctgc agcgtgtcga
    17581 tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg gacatctgta gcacgtttca
    17641 gcggctggac atcttccatg gcgtcaagaa acagcgattt gtcgtcaagg ttcatgtaac
    17701 ctcctatgca ccttcacacg tcgtgattca gacgggttaa ttgttcatac ttactgcatc
    17761 gttcccttca cagattgaaa caatactcta cttcatacct gtgagtatct gcgcaggcgg
    17821 ggcattttat actagcctgt tagcagtatc gaacagttta gcccgagttt aattcagcac
    17881 aggctccgat caggatgatt tattcatagc aggaaccaac aaaaatgcga taaataacag
    17941 accactgatt agcagaaagg taccgaaaat gccatagttt gcgcctccct cagcatcaat
    18001 tgactcaata atttggttga agccagtatt tccgatgtct ggtcacttct actgcgatta
    18061 aaaaataaag cacttctgga aatgaagtca ttcaccgtat ggggaacaga aaccattttt
    18121 tcacccattc tcgaaaaatg aaaattagtt cgattattga gaatagagcc acacagggca
    18181 ataccaatcg cgcccccaag attacgtgtt aaattaaata caccagaggc taacttcagc
    18241 cgttcttttg gaattccccc cagcgttaat gttacgatgg gggccatcgc aaactgctgc
    18301 aaaatccctc taattgcctg tggaaacagt aattcctgcc atccccattc gtgggttata
    18361 ggggtaaaaa gatacattga aaacacaaat ccgcccagcc ccgccatcaa cagccatcgc
    18421 agatttattt ttttacttaa ccagaagtaa aagggaacag aaaataactg gaaaatacct
    18481 gttgtgcaaa cggcaaaacc tatctcttcg gcatttaatc cgcgaatctg ccccaaaaac
    18541 acaggtatta aatatacagt agagaatatc ccaacccctc cagaaaaaga aaaaaaacat
    18601 cccagagtga aatttttatc cttaaaagca tgaagatcca ttatcggatt ggatattgta
    18661 agtgtacgcg cagcaaataa aataaaactc accaatgcca gaactgatgt cagcaaaata
    18721 gtattatcat ccagccatcc ccagcgcgct ccttcttcaa gggtatattc cagacatccc
    18781 agagtcatgg ccaacaggat aatactcgga taatcagcaa ctttcagtaa agagagatca
    18841 ggtttatcaa aattaaccag aaaaggaata ctcaaaacta aatatatgcc aggtaatata
    18901 ttgatataaa atagccatcg ccagtcgagg ttatcagtga gccagccccc cagagttggc
    18961 cctagcgtcg gtgacagtga ggccagcgca ctgactaccg cagcagccaa ccccaactct
    19021 ttaccctggt aataaataaa cgccgtcgtg aaaactaaag ggatcatgga tgcgccagca
    19081 gctccctgta aagcacgaaa caaaatcatg atctgaatat tccatgcaag tccacacgcg
    19141 atactcatta aagtaaatat tcccgcggat agagtaaaaa gccaacgagt ggaaaatacc
    19201 cgtgacagcc acccagagag cggaataaca ataatctcag caattaaata gctggtctga
    19261 agccaggcca tttcgtcttt acctgcggac agaccaccac ctatttcatt tagggatgac
    19321 gatacaattt gaatatcaag ctgagcaata aataatccca tacacatact ggcgaaagca
    19381 atatgtttac gcatagacat catctctcat caacactgac ggtgacggac agtcccgggc
    19441 gaagaagttg ctttaattcc tttgcgtcgt caaatgcaat ccgtaccggg acacgctgaa
    19501 caattttggt gaaattgcca gtggcatttt ctatcggaat caagctgaaa ctggctcctg
    19561 tcgctgggga aaggctttca atgtgaccgt gaaacacttc ccctttgaga atatcagcac
    19621 ggatctcagc cttcattcct ggcttcattc ccgctatttg gttttcttta taatttgcat
    19681 caacccacaa ctcactgact ggtactagtg aaactaacga tgtgccgcct tccacccaac
    19741 tgcc
//
LOCUS       DAJTYF010000072        19293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTYF010000072 DAJTYF010000000
VERSION     DAJTYF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.072"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(56..1885)
                     /gene="glmS"
                     /locus_tag="OO590_002611"
     CDS             complement(56..1885)
                     /gene="glmS"
                     /locus_tag="OO590_002611"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6637927.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2047..3417)
                     /gene="glmU"
                     /locus_tag="OO590_002612"
     CDS             complement(2047..3417)
                     /gene="glmU"
                     /locus_tag="OO590_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6637928.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGAGMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3769..4188)
                     /gene="atpC"
                     /locus_tag="OO590_002613"
     CDS             complement(3769..4188)
                     /gene="atpC"
                     /locus_tag="OO590_002613"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6637929.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4209..5591)
                     /gene="atpD"
                     /locus_tag="OO590_002614"
     CDS             complement(4209..5591)
                     /gene="atpD"
                     /locus_tag="OO590_002614"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6637930.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5618..6481)
                     /gene="atpG"
                     /locus_tag="OO590_002615"
     CDS             complement(5618..6481)
                     /gene="atpG"
                     /locus_tag="OO590_002615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6637931.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6532..8073)
                     /gene="atpA"
                     /locus_tag="OO590_002616"
     CDS             complement(6532..8073)
                     /gene="atpA"
                     /locus_tag="OO590_002616"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6637932.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8086..8619)
                     /gene="atpH"
                     /locus_tag="OO590_002617"
     CDS             complement(8086..8619)
                     /gene="atpH"
                     /locus_tag="OO590_002617"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6637933.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8634..9104)
                     /gene="atpF"
                     /locus_tag="OO590_002618"
     CDS             complement(8634..9104)
                     /gene="atpF"
                     /locus_tag="OO590_002618"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6637934.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9166..9405)
                     /gene="atpE"
                     /locus_tag="OO590_002619"
     CDS             complement(9166..9405)
                     /gene="atpE"
                     /locus_tag="OO590_002619"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6637935.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9452..10267)
                     /gene="atpB"
                     /locus_tag="OO590_002620"
     CDS             complement(9452..10267)
                     /gene="atpB"
                     /locus_tag="OO590_002620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6637936.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10276..10656)
                     /gene="atpI"
                     /locus_tag="OO590_002621"
     CDS             complement(10276..10656)
                     /gene="atpI"
                     /locus_tag="OO590_002621"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6637937.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11273..11896)
                     /gene="rsmG"
                     /locus_tag="OO590_002622"
     CDS             complement(11273..11896)
                     /gene="rsmG"
                     /locus_tag="OO590_002622"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6637938.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11960..13849)
                     /gene="mnmG"
                     /locus_tag="OO590_002623"
     CDS             complement(11960..13849)
                     /gene="mnmG"
                     /locus_tag="OO590_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6637939.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14228..14671)
                     /gene="mioC"
                     /locus_tag="OO590_002624"
     CDS             complement(14228..14671)
                     /gene="mioC"
                     /locus_tag="OO590_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6637940.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14761..15219)
                     /gene="asnC"
                     /locus_tag="OO590_002625"
     CDS             complement(14761..15219)
                     /gene="asnC"
                     /locus_tag="OO590_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6637941.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15371..16363
                     /gene="asnA"
                     /locus_tag="OO590_002626"
     CDS             15371..16363
                     /gene="asnA"
                     /locus_tag="OO590_002626"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6637942.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16368..17819)
                     /gene="viaA"
                     /locus_tag="OO590_002627"
                     /pseudo
     CDS             complement(16368..17819)
                     /gene="viaA"
                     /locus_tag="OO590_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17813..19279)
                     /gene="ravA"
                     /locus_tag="OO590_002628"
     CDS             complement(17813..19279)
                     /gene="ravA"
                     /locus_tag="OO590_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6637943.1"
                     /translation="MSRLSSSLEKGLYERSHAIRLCLLAALSGESVFLLGPPGIAKSL
                     IARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTSGYLPEAEIVF
                     LDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEADSSLEALYDR
                     MLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKEIGEITLPDHV
                     FELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVDLILLKDCLWY
                     DAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKTALTLIRLGGI
                     FSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLSKGGEIRGKLN
                     GIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQLEELESDWRKQ
                     HALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4605 a         5113 c         4835 g         4740 t
ORIGIN      
        1 ggtcaactga tcggcattac accggtcggc agcccttttt tatctatgat agtgattact
       61 caaccgtaac cgattttgcc aggttacgcg gctggtcaac gtcggtgcct ttgatcagcg
      121 cgacatggta agccagcagc tgcagcggaa cggtgtagaa gatcggtgca atcacctctt
      181 ccacatgcgg catctcgatg atgtgcatgt tatcgctact tacaaaaccc gcatcctgat
      241 cggcgaagac atacaattga ccgccacgcg cgcgaacttc ttcaatgttg gatttcagtt
      301 tttccaacag ttcgttgttc ggtgcaacga cgataaccgg catatccgcg tcaatcagcg
      361 ccagcgggcc gtgtttcagc tcgcctgcag cgtaggcttc agcgtgaatg taagagatct
      421 ctttcagctt caatgcgcct tccagcgcga ttgggtactg atcgccacgg cccaggaaca
      481 gcgcgtgatg tttgtcagag aaatcttctg ccagagcttc aatgcgttta tcctgagaca
      541 gcatctgctc aatacggctc ggcagcgcct gcagaccatg cacaatgtca tgttcaatgg
      601 aggcatccag acctttcagg cgagacagct tcgccaccag catcaacagc acagttaact
      661 gagtggtgaa tgctttagtg gacgccacgc cgatttccgt acccgcgttg gtcatcagcg
      721 ccagatcgga ttcgcgcacc agagaagaac ccggaacgtt acagattgcc agcgaaccaa
      781 ggtaacccag ctctttcgac agacgcaggc ccgccagggt atccgcggtt tcgccagact
      841 gtgacaaggt gatcatcagg ctgttacgac gcacggcaga tttacgatag cggaattcag
      901 aggcgatttc gacgtcgcac ggaatacctg ccagcgattc aaaccagtag cgggaaacca
      961 taccggagtt ataagaagta ccacaggcga ggatctgaat atgctcaacc ttcgacagca
     1021 gttcgtcggc gttcggtccc agctcgctta aatcaacctg accgtggctg atgcgcccgg
     1081 taagggtgtt ttttatcgcg ttcggctgtt cgtagatctc tttctgcatg tagtgacggt
     1141 aaataccttt atcgcccgcg tcatattgca gattggattc gatatcctga cgttttactt
     1201 ccgcgccagt tttatcgaag atgtttaccg aacggcgagt gatttccgca atatcgccct
     1261 cttcaaggaa gataaagcga cgggtcaccg gcaacagcgc cagctggtca gaagcgataa
     1321 agttttcgcc catacccagg ccaatcacca gcggactacc agaacgtgcc gccagcaggg
     1381 tatccgggtg acgggagtcc atgatcactg taccgtacgc accgcgcagc tgcgggatag
     1441 cacgcagaac agcctcacgc agagtcccgc cttgtttcag ctcccagttc accagatggg
     1501 caatcacttc ggtgtcggtt tcagaaacga aggtatagcc acgcgctttt aactcttcac
     1561 gcagcggttc atggttttcg atgatgccat tatgcaccac cacaatgtgt tcagaaacat
     1621 gcggatgcgc gttagcttct gaaggttcac cgtgggtcgc ccagcgagtg tgagcaatac
     1681 cggtgccgcc atgcagagga tgttcttccg ctgcctgagc cagcatctgg actttaccga
     1741 ggcgacgcag gcgagtcata tgaccttccg catcaacaac ggccagaccg gcagagtcat
     1801 atccgcggta ttccagacga cgtaaacctt caagaaggat ttctgctaca tcacgttgcg
     1861 cgatcgcgcc aacaattcca cacatagttt ttgattccga tttatatcgt tgtcggtcaa
     1921 cctgtatgcc cgttttttcg ggcgccccga gccttgtaga gagtggggtt atttttatag
     1981 ttactgcttg tgggagggag attatgttat ctcctcatcc cagatgaccg gattacccgg
     2041 ccagaattac tttttcttta ccggacgacg ccagccttct ttctgagtct gcggcacacg
     2101 gctgatagct aatgcatttt cgccgacatt acgcgtcaca gttgtacccg cagcaatggt
     2161 cgcgcctttg cctactgtta ccggggccac cagctgagtg tcggaaccga caaacacatc
     2221 gtcgccgata atggtcttaa atttattcgc accatcgtag ttgcaggtaa tggttcccgc
     2281 gccgatgtta acgttatcgc caatttccgc atcgcctagg taagtcagat gcccggcttt
     2341 cgtgccttta cccagacgcg cttttttcat ctcaacgaag ttaccaacgt gtgcaccttc
     2401 cagcaactca gcaccaggac gcagacgggc aaacgggcca atagtacagg ccgctgccag
     2461 attcgcgtct tccacaacgg tatacggact gatttcgcaa tcatcgccaa tcacgctgtt
     2521 tttaatcacg caaccggtgc cgattttcac gcgatgaccg agagtcacgt tgccctcgat
     2581 gataacgtta gtatcaattt caacatcgcg cccgtgagta agcgtaccgc gcagatcaaa
     2641 acgcgccgga tcgcgcagca taacgcctgc taacagcagt ttttcagcct gttcggactg
     2701 gtaaacgcgc tccagacggg agagttgcag gcggttattc acaccttcta cttcgcttaa
     2761 acgttgcgga tgaacggcga cgatttcacg cccttcctga tacgccagcg caataatgtc
     2821 ggtgatgtag tattcgccct gagcattatt gttggtcagc ttcgccagcc agcgtttcat
     2881 acctgcgccg ttggcaatca gaatgccggt gttgatctcc tgaatctgac gctgctcgtc
     2941 ggtggcatct ttgtgctcaa caatgccggt aactttgcca ttttcacggg tgatacgtcc
     3001 ataaccggtc ggatcatcca gtttcaccgt cagcagacca atgccaccct ggggtttagc
     3061 atcacgcagg cgctggagtg tttcgacaga gatcagcggc acgtcgccgt agagcattaa
     3121 aatgtcttca tcatcggcaa agaaaggtgc ggcctgctgc attgcatgac ccgtacccag
     3181 ctgctctgcc tgaagcaccc agttaaggtt gtcgtctttc agcgcctgtt taagcagatc
     3241 gccgccgtga ccgtacacca ggtgaacgtg cgctgcgcct aattcattcg cagcatcaat
     3301 gacatgctga accatcgctt tcccggcaag ggtatgcagc actttcggaa gatcggaata
     3361 catgcgcgtg cctttgcctg cggcaaggat cactacgctc atagcattat tcaacatacg
     3421 cgtcctgact gtaatttgag aacgaattta aaccgcttca ccttgaaaaa actacatttt
     3481 tttcatcgtg aaatggacag aggataaatt gttcaataac ggattatccc cgctgacgaa
     3541 agtgccattt tcgaccatcg tcacgccttt tgtctgcaga aaattaaggc agtaccttca
     3601 tttaagcgtc aggtggatgt ttttgctctt atttcgatca atgaataaac gggaaaacag
     3661 gggggtttta tatcagcagg atctatgtga acgctcttca ggacgggtca acgcgtaaaa
     3721 aaaagccagc ctgtttccag actggctttt gtgcttttca agccggtgtt acatcgcttt
     3781 tttggtcaac tcgataacgc gcagctgcgc gatcgctttg gccagttccg cagacgcctg
     3841 agcgtaatct acgtcgccgt gagagctgct aatgtgctct tcagccttac gtttcgcttc
     3901 catggctcgc gcttcgtcga gatcctggcc gcgaattgca gtgtcggcca gaacggtcac
     3961 gttgccaggc tgcacttcaa gaatgccgcc agacagatag ataaactctt cgtgaccgtg
     4021 ctgtttcacg atgcgaatca taccaggctt aatggcggtg agcagcggtg cgtggccagg
     4081 gtagatcccc agttcacctt cgctacccgt tacctggatt ttctcgacca gaccagagaa
     4141 catttgttgc tctgcgctga cgacgtccag gtggtaagtc attgccatat caccctccga
     4201 ttaaggcgtt aaagtttttt ggctttttcc acagcttctt cgatggaacc gaccatgtag
     4261 aacgcctgct ccggcaggtg atcgtattcg ccttccatga tgcctttaaa gccacggatg
     4321 gtgtctttca gggagacgta tttacccgga gaaccggtga atacttctgc cacgaagaac
     4381 ggctgggaca ggaagcgctg gatcttacga gcacgcgcta ccaccagttt gtcttcttca
     4441 gacagttcat ccatacccag gatggcgatg atgtctttca gttcctgata acgttgcagg
     4501 atggactgaa cgccacgcgc ggtgtcgtag tgttcctgac caaccaccag cgggtccagc
     4561 tgacggctgg tggagtccag cgggtcaacg gccgggtaga tacccagaga cgcgatctga
     4621 cggctcagta ccacggttgc gtcaaggtgc gcaaaggtgg ttgccggaga cgggtcagtc
     4681 aagtcatccg caggtacgta taccgcctgt acggaggtga tagaaccagt tttggtggag
     4741 gtgatacgtt cctgcagaac gcccatctct tccgccaggg tcggctgata acctaccgct
     4801 gaaggcatac ggcccagcag tgcggatact tccgtaccgg ccagggtgta acgatagatg
     4861 ttgtcaacga acagcagaac gtcacgacct tcgtcacgga atttctcagc catggtcaga
     4921 ccggtcagag caacgcgcag acggtttccc ggcggctcgt tcatctggcc atacaccagg
     4981 gatactttgt cgataacgtt ggagtcggtc atttcgtggt agaagtcgtt accctcacga
     5041 gtacgttcac ctacgcccgc aaacacagag taaccggagt gctcgatcgc gatgttacga
     5101 atgagctcca tcatgtttac ggttttacct acacccgcac caccgaacag accaacttta
     5161 ccgcccttag cgaacggaca catcaggtcg ataactttga taccggtttc cagcagttcc
     5221 tgagagtttg acagctcttc gtaggaaggt gctgcgcggt gaatcgccca acgctcttct
     5281 tcaccgatct cgcctttcat gtcgaccggt tcacccagta cgttcatgat acggcccaga
     5341 gtcgctttac ctaccgggac ttcgatcggg tgttcgaggt cttttacatc cagaccgcga
     5401 cgcagaccgt cggaggaacc cattgcgatg gtacgcacga taccgccgcc gagctgctgc
     5461 tgaacttcca gcaccagacg ctcattacca ttttgcacct caagagcatc gtacacgcgc
     5521 ggtacggcat cctgagggaa ttcgacgtca actacggcgc cgattacctg gacaatcttt
     5581 ccagtagcca tcttaaatcc tctacgaaat aacctgttta aaccgcggcg gcccccgaga
     5641 cgatctcggt gagttcctga gtaatgctgg cctgacgagc tttgttgtat accaactgca
     5701 gctctttaat caggctgccg ccattgtcgg tcgcggcttt catcgccacc atacgggcgg
     5761 cctgctcgct ggccaggttt tcaaccacgc cctgataaac ctgagattcg acataacgac
     5821 gcagcagggt atccagcaac gccttcggat cgggttcgta caggtaatcc caggatttat
     5881 gtttcagatc atcatcatct gatgccggta acggcagcag ctggctgatg gtcggaacct
     5941 gagacatggt gttaataaat ttgttgctga caatgtaaag cttgtccaga cggccttcgt
     6001 cgtaggcctg caacatcact tttaccggac cgatcagttc ggacagggaa gggttatccc
     6061 ccatgccggt gacctgggca acaacattgc cgcccacgga gttgaagaac gacacgcctt
     6121 tcgagccgat cattgcgagg tcgcattgaa cgcctttgtc ggtccaggtc ttcatttccg
     6181 ccagcagttt tttgaacagg ttaatgttca aaccaccgca caaaccacgg tcggtcgaca
     6241 ccaccaggta gcccacgcgt ttaacgtcgc ggtcttccag gtaagggtgc ttatattcca
     6301 gattaccgtg tgcaaggtga ccaatcactt tgcgcatggt ttctgcataa ggacggctgg
     6361 ccgccatgcg atcctgcgat ttacgcattt tggaagcggc gaccatctcc atcgctttag
     6421 tgatcttttg cgtgttctgg acgcttgcga tcttactacg tatctctttt gcgccggcca
     6481 tgagcttctc ctcaatgcct tgcggcctgc cctaaggcaa gccgccagac gttaccagga
     6541 ttgggttgct ttgaaggaat cgaggatgcc tttcagcttg ccttcgattt cgtcgttgta
     6601 gccaccggtc tggttgatct cttgcatcaa cggagcgtga tcacggtcga cgtaagccag
     6661 cagagcggct tcgaagctgc caattttcga cagttcaaca tccgccaggt aaccacgttc
     6721 tgctgcgaac agaaccagag actgctgcgc aacggacatc ggcgcatact gtttctgttt
     6781 cagcagttcg gtcactttct gaccgtggtc aagctgctta cgtgttgcat cgtcaaggtc
     6841 ggatgcaaac tgagagaacg ctgccagttc acgatactgt gccagagcgg tacggatacc
     6901 accggacagt tttttcatga tcttggtctg tgctgcacca ccaacacggg atacggaaat
     6961 acccgggtta accgcaggac gaataccggc gttgaacagg ttggtttcca ggaagatctg
     7021 accatcggta atggagatta cgttggtcgg aacgaacgca gaaacgtcac ccgcctgagt
     7081 ttcgataatc ggcagtgcgg tcagagaacc ggttttccct ttcacttcac ctttggtgaa
     7141 ggcttcaacg tattcggcgt taacacgtgc agcacgctcc agcagacgag agtggaggta
     7201 gaaaacgtcg cccgggaatg cttcacgtcc tggcggacga cggagcagca gggagatctg
     7261 acggtaagca acagcctgtt tagacaggtc atcgtaaatg atcagcgcat cttcaccgcg
     7321 gtcacggaag tattcgccca ttgcgcaacc ggcatacggt gccaggtatt gcagtgcagc
     7381 ggattcagac gcggttgcta ccacaacgat ggtgttagcc agtgcgccgt gctcttccag
     7441 tttacgtacc acgttagaaa tggtggacgc tttctggccg atagcgacat agatacattt
     7501 gataccggaa tcgcgctggt tgatgatggc atcgatagcc agtgcggttt tacctgtctg
     7561 acggtcaccg atgatcaatt cacgctgacc acgaccgatt gggatcatgg agtcaacggc
     7621 tttataaccg gtctgtaccg gctgatctac ggactgacgt tcgataacgc ccggagcgat
     7681 tgcttctaca gcagagaagc cgtcgtgatc cagcggacct ttaccgtcga ttggtgcacc
     7741 cagagtgtta accacacggc ccagcaggcc acggccaacc ggaacttcca ggatacggcc
     7801 agtacactta actttcatgc cttcggcaag gtcagcgtac ggacccataa caaccgcacc
     7861 tacagagtcg cgctcaaggt tcagtgcgat agcgtaacgg ttacccggca gggagatcat
     7921 ttcaccctgc atacaatcgg ccaggccgtg aatgcggata acaccgtcac ttacagaaac
     7981 aatagtacct tcgttgtgag cttcactcac aacattgaac tgagcaatgc gctgcttgat
     8041 cagttcgctg atttcggtgg aattcagttg catgctccag tccccttaag actgcaagac
     8101 gtctgcaagg cgctcaagac gaccgcgtac gctgccatca atgaccatat cacccgctcg
     8161 gatgataacg cctgccatta cagacttatc gattttgcaa ttcagcttaa ctttgcgtga
     8221 cagacgtttt tccatcgcag cagaaatttt cgcgagctgt tgttcactca gtgcggcagc
     8281 ggaaatgacg tctacctcag cggtagcctc actcacggca cgaaggtgaa taaactgctc
     8341 cagaacatcc gggagcgcgt taagacgacc attttcagcc attacccgaa tcaggttctg
     8401 accgttttcg tccagttgct caccacaaac tgcgataaac gactcggcga gcgtttctgg
     8461 cgcaagcgcg ccagagagaa gctctgccat ttgttcgttt ttggttacct cggcggcaaa
     8521 cgccagcatg tcctgccagc gttctacact ttggtgttcg acggcaaagt caaaagctgc
     8581 tttggcgtag gggcgagcta ccgtaataaa ttcagacatc agcccctccc tccttacagt
     8641 tcagcgacaa gtttatccac gatgtcgctg ttagcagctt catccacgga acgttcgatg
     8701 atcttctcgg cgccagcaac agccaggata gcaacttgct tacgcagctc ttcacgggca
     8761 cgtttacgct cggcttcaat ttcagcctgc gcctgggcca cgattttagt acgttcctgt
     8821 tctgcctcag ctttcgcttc gtccagaatc tgcgagcgga gtttattcgc ctgctcgatg
     8881 attacctggg cttccgcttt cgcttttttc agctggtcgg tcgcgctggc ctttgcaagg
     8941 tcaaggtcct tatgtgctcg ttctgcggaa gcaaggccgt cagcaatttc tttttgacgt
     9001 ttttcgatgg ctgccattaa tggcggccat acgtacttca tgcagaacag aacgaacagg
     9061 acaaacgcga tggcctggcc gaggattgtt gcgttaagat tcacagcaca atgcctctat
     9121 ttagttaacg ttctgatatt gctctttaaa taaaagcaac gcttactacg cgacagcgaa
     9181 catcacgtac agacccagac ctacagcgat catcgggata gcatccacca gacccataac
     9241 gataaagaac tgagtacgca gcagaggaat cagatcaggt tgacgcgctg cgccttccag
     9301 gaatttaccc ccgaggatgc cgataccgat cgcagcaccg attgccgcca gacccatcat
     9361 cacagcggca gccatgtaca gcagatccat attcaggttt tccatgacag tctccagttt
     9421 gtttcagtta aaacgtagta gtgttggtaa attaatgttc ttcagacgcc atcgacagat
     9481 agacgatcgt cagaaccatg aagatgaagg cttgcagcgt aatgatcagg atgtggaaaa
     9541 tggcccacgg cacattcagg atccactgtg accaccacgg caacagacca gcaatcagaa
     9601 tgaaaatcag ctcaccggca tacatgttac cgaacagtcg caaaccgagt gaaactggtt
     9661 tggacagcag gcttacccct tcaaggatta agttgacagg aatgaacgcc cagtgattga
     9721 acggctgcag cgtcaactct ttcgtgaagc cgccgatgcc tttcattttg atgttgtaga
     9781 acagaatcag gataaatacg cccagtgcca tagacagcgt tacgttcacg tccgcagacg
     9841 gaaccacacg cagtgcaggc agacccagta catgttcagc aatgtacggc agcaagtcga
     9901 taggcagtaa atccatcaga ttcatcagga atacccagac gaagatcgtc agggccagcg
     9961 gagcaatcag cttgcttttg ccatggtaca tgtctttcac gctaccatta acaaagccga
    10021 tcaccagctc aatcgcggtc tgaaacttac ctggcacacc gctggtcgcc tttttggcta
    10081 cgctacggaa taaaaccaag aacaacagac ccagcaccac cgagaagaac atggagtcaa
    10141 tattgattgt ccagaaggtg gctggggggt tttgtggatc caccagcgag aatgtacgca
    10201 ggtccagctg aaggttattc aggtggtgtc ctatgtaatc ctgcggcgtc atattttctg
    10261 aagccatgat gccttttacc ctttgttgtt aattacagcc ggtgccagta tctgaaccac
    10321 cagcaccaaa acccacgtaa cgatcagcgg caagaatacc gcctttaaaa ccgccaacgc
    10381 caccaccagt aacaccaaca tcgccagaac tttgaaagct tcgccaaatg cgaatgtcca
    10441 agccacccgg cctttcgctg gtgtatgcgc ctggtgacgc caggcaaata tcataaacaa
    10501 aacgttaggc agaaagactg ccaggccccc gcttattgca gagacgcccc agaaggggtc
    10561 tttgaggctg aacagcaatc cacttgctat caccaccagt aactgaacga gcagaagctt
    10621 ccgagcaacg tttcgactca cgagcgacac agacatcacg tttttcactc ctgctccctt
    10681 cgaggtatgc cgcgtgtcgt ataaaacttt ctttaaggct tagagtcaag catcaaaaag
    10741 cggtcaaatt atacggtgcg cccccgtgat ttcaaacaat aagtagccaa aaggtgaata
    10801 aatgtttaaa tatttttcca gtgcatacaa ttgcgacttt tccgctaccc ctgttcgatc
    10861 atgaaaaact gtaaataacg cgtaaacact ggtgataaag cgtgcttcag atcacatatt
    10921 gcgcatgttc gcgcacagca tatttattta cttggcaaat gatgcctttg caagtttatg
    10981 atatttcagt ctaaaaacag atactgtttt aataaatgac atttacacaa caaaaaccac
    11041 ccattgacat ttttaataat gttttaacag ccaatgatgg ttcttagcgc cgatttttag
    11101 cagactgata ttttcactaa tgacttattt tctgcttacc aaaaaaagcc acgttatctt
    11161 gttgatgcaa aagagtgaac gtggcgttaa atgtaaccag ttatatcagt agaaaacctg
    11221 gttgttgtta acagtctaac cggtcaattt tttatgattt ttttgataaa aattaaattt
    11281 tatttgcttt aatcaccacc agatgacgtt cgccatccag ggctggaacc tgaagtttaa
    11341 ccactgattc gacctgatat tcttcgggca acaaagcgat ttcatcttcc ggcatttgcc
    11401 ctttcagcgc gtagaaacgg ccttgctcac caggaagatg gtggcaccag ctcaccatat
    11461 cgttcagaga ggcaaaagcg cggctaatta cgccatcaaa tggcggctct gaaggaaact
    11521 cttctaccct gctctgtact ggttcaatat tctccagttt aagctcatgt tgcacctgac
    11581 gaaggaaacg cacgcgttta ccaaggctat ccaacagagt gaaatgggct tcaggacgca
    11641 cgatagagag tggaatgcct ggcagtcctg gtccggtgcc gacatcgata aaccgttcac
    11701 cttgcagata cggtgccacc acaatgctat cgagaatatg gcgtaccagc atctcattag
    11761 gatcgcggac cgaagtcagg ttgtacgctt tgttccattt atgcagcata ttcacgtagg
    11821 caataagctg gtttttctgg tgatcagtaa gcgaaatacc tgcgtctttc agcagtaagg
    11881 agagtttgtt gagcacggtg attacctgtt cttgatgcgt tgcttggtaa gcgggtgctt
    11941 accaggcatt tttaatgcgt tatgcgctac gacgcagcat accctgtttt ttcagccaca
    12001 ccagcagaat ggagatggcc gcaggcgtga cgccagaaat acgcgaagcc tggccgatag
    12061 aggccggttt gtgatcgtta agtttggcga tcacttcgtt agaaagaccg gatacctggc
    12121 ggtaatccag tgtcgcgggt agcagggtgt tctcgttacg cagctgcttt tcgatctcat
    12181 cttgctggcg cgcgatataa ccttcgtatt taacctgaat ctcaacctgt tccgccgcct
    12241 gttcgtctgt caacgcaggg gcaaacggcg tcagcgtggt taatttttca taagtcattt
    12301 ccggacgacg cagcagatct tcaccactgg cttcacggga aagcggcgca gtcaggtgag
    12361 cattcacttc ggctgcagct tccgccgacg gggttaccca ggtcgatttc agacgctgac
    12421 gctcacgctc gatattctca agtttctcgt taaagcgcgc ccaacgttcg tcatccacca
    12481 ggcccagttc acgaccgatt tcagtcaaac gcagatccgc attatcttcg cgcagcatca
    12541 gacgatattc tgcgcgcgaa gtaaacatac gatacggttc tttggttcct aaagtgcaca
    12601 ggtcatcaac cagtacgccg agatacgcct gagaacgtgc cggagcccaa ccttctttgt
    12661 cagcagacag acgggcagcg ttaagaccgg ccagcaaacc ttgcgcagcg gcttcttcgt
    12721 aaccggtagt gccgttaatc tgaccagcaa agaacagccc ctggataaac ttgctctcca
    12781 gcgtcggttt caggtcgcga ggatcgaaga agtcatactc aatggcataa cccggacgca
    12841 cgatcttcgc gttttccatc ccctgcatag agcggacgat ttgcatctgc acatcgaacg
    12901 gcaggctggt ggagataccg ttcggataaa tttcattaga ggtcagtcct tccggttcaa
    12961 ggaagatctg atgctgattt ctgtctgcga agcgcatgac tttgtcttcg atcgacgggc
    13021 agtagcgtgg gccgacacct tcgatcaccc ctgcatacat tgggctacga tcgaggttac
    13081 tgcggatcac atcatgggtt ttctcgttgg tatgagtgat ataacacggc acctgctggg
    13141 gatgcaggga cgcattgccc ataaacgaga ataccggcat tgggttatcg ccatgttgtt
    13201 gcgccagcac gctaaagtcg atggttcgag catcaatacg cggcggagta ccggttttca
    13261 gacgaccaac gcgcagcggc agttcacgca aacggcgaga gagcggaatg gatggcggat
    13321 cgccagcacg gccaccgctg taattatcca gaccgatatg aattttgccg tcgaggaacg
    13381 tcccaacggt gagcaccaca gctttggcac ggaacttcag tcccatttgg gtaacagcac
    13441 cgaccacgcg atcgttttcg acaataagat cttcaaccgc ctgctggaag atcatcaggt
    13501 tcggttggtt ctccagcgcc gtacgtaccg cctgacggta gcgcacacga tccgcctgag
    13561 ctcgggtagc gcgaaccgcc ggtcctttgc ttgcgtttag tatcctaaac tggatacccg
    13621 cttgatcgat cgctttcgcc atcagaccgc cgagtgcatc cacttctttt accagatgtc
    13681 ccttcccaat accgccgatc gccgggttgc agctcatctg ccccagagtg tcgatattgt
    13741 gtgtcaaaag cagagtctgt tgacccatac gcgccgcagc catcgcggcc tcggtgcctg
    13801 catgaccccc gccaatgatg atgacgtcaa aaggatccgg ataaaacatg gtgattgcct
    13861 cgcataacgc ggtatgaaaa tggattgaag cccgggccgt ggattctact caactttgtc
    13921 ggcttgagaa agacctggga tcctgggtat taaaaagaag atctatttat ttagagatct
    13981 gttctattgt gatctcttat taggatcgca ctgccctgtg gataacaagg atccgacttt
    14041 taagatcaac aacctggaaa ggatcattaa ctgtgaatga tcggtgatcc tggaccgtat
    14101 aagctgggat cagaatgagg ggttatacac aactcaaaaa ctgaacaacg gttgttcttt
    14161 ggataactac cggttgatcc aagcttcctg acagagttat ccacagtaga tcgcacgatc
    14221 tgtatactta tttgagtaaa ttaatccacg atcccagcca ttcttctgcc ggatcttccg
    14281 gaatgtcgtg atcaagaatg ttgatcttca gtgtttcgcc tgtctgtttt gcaccggaat
    14341 ttttgagttc tgcctcgagt ttatcgatag ccccacaaaa ggtgtcatat tcacgactgc
    14401 caataccgat tgcgccaaag cggaccgcag aaagatcggg cttctgttcc tgcaatgctt
    14461 catagaaagg agaaaggttg tccggaatat ctccggcacc gtgggtggag ctgataacca
    14521 gccagatccc cgaggcagat aaatcttcca acagcggacc gtgcagcgtt tcggtggtaa
    14581 aacccgcctc ttccagcttt tcagccaggt gttctgctac atattcggca ccgccgaggg
    14641 tgctgccgct gataagagtg atatctgcca taaaccgcca cctttattaa gagtggcgta
    14701 ttgtacgctg tgaacgcgtt gggatctacc tgtggaaaag tatgggatta aaaaagccga
    14761 tcagggcttg atggtacgca tgatcgggtt ttgcaggacg atcaatgtct cggtggactg
    14821 aatttcatca attgtttgga tcttgttgat aagtacatgc tggagagcgt cgatcgaacg
    14881 gcacataact tttataaaga tgctgtagtg gccggttgtg taataggctt cagtgacttc
    14941 atcaaggctt tccagctttg ccagcgcgga agggtagtct ttggcgctct ttaatataat
    15001 gccgataaag cagcctacgt cataaccgag ctgcttcggg ctgacatcaa tacgcgcccc
    15061 ggtaatgatc cccgcctgct tcattttctc tactcgaacg tgaatcgtcc ccggactgac
    15121 gccaaattgt ttcgccagtt cggcgtaagc ggtgcgcgca ttgcccatta atgcttccag
    15181 gatgccacgg tccagattgt cgatcagata attttccata ggattttctt atgcagattg
    15241 atgattcatt ctattttagc cttctttttt aatgaatcaa aagtgtgtta ggctttttat
    15301 tgaatgatta ttgcatgtgt gtcggttttt gttgcttaat cataagcaac aggacgcagg
    15361 agtataaaaa atgaaaaccg cttacattgc caaacaacgc caaattagct tcgtgaaatc
    15421 tcacttttct cgccaactgg aagaacgtct ggggctgatc gaagtccagg cgccgattct
    15481 tagccgtgtg ggggatggca cgcaggataa cttgtcgggc tgtgaaaaag cggtgcaggt
    15541 aaaagtgaaa gctctgcctg atgcccagtt cgaagtggtt cattcactgg cgaagtggaa
    15601 acgtcagacc ttagggcaac acgacttcag cgcgggcgaa gggctgtaca cgcacatgaa
    15661 agcccttcgc cccgatgaag accgtctttc tccgttgcac tcggtctatg ttgaccagtg
    15721 ggactgggaa cgcgtaatgg gcgacggtga gcgtcaattc tcgactctga aaagcacggt
    15781 agaggcgatc tgggcgggaa ttaaagcaac cgaagctgcg gttaacgaag agtttggcct
    15841 ggcaccgttc ctgccggatc agattcactt cgtacacagc caggagttac tgtctcgtta
    15901 tccggatctc gatgccaaag ggcgtgagcg ggcgattgcg aaagatcttg gcgcggtatt
    15961 cctcgtcggg attggcggca agttgagtga tggtcatcgt cacgacgtgc gcgcaccgga
    16021 ttatgatgac tggagcaccc cgtcagagct gggccatgcg ggcctgaacg gcgatattct
    16081 ggtgtggaac ccggtactgg aagatgcgtt tgagctttct tccatgggga tccgcgtgga
    16141 tgccgacacg ctgaagcatc agctggcgct gaccggtgat gaagatcgtc tgcagctgga
    16201 atggcatcag gcgctgctgc gcggtgaaat gccgcagacc atcggcggcg gtatcggcca
    16261 gtctcgtttg actatgctgc tgctgcaact gccgcatatc ggccaggttc agtgtggagt
    16321 atggccagtt gctgttcgcg agagcgtccc ttctctgctg taataattta tcgccgccag
    16381 cgtctgagca ggcggcttcg catcccggta tcaaagcgcc agatatgatc gaaaatgcgc
    16441 atgatgccgg gtttgccgtg tgccgacatc gccacggcat gaaagcgatg ctgatgtacc
    16501 cgctgcagct ctttcacttt actcgtcacg tcgtcaggca accgctgagc gataaaatca
    16561 gaaatcacca ccgcatcggc atcaaaccat tccctgcttt gcaagcgttc cataatggcg
    16621 cgaaaacaac tggcaagatc ggtgccgcca cgaaactgct ggcttaaaaa acggattgct
    16681 tgttcgatgc cttgtgggcc tgaaagctca taacggacga tctcggtgga aaatagcata
    16741 atataacagc gccggttttc agcgagagca atgcgcatca aagccaggca gaacgctttc
    16801 gcacactgtt cattaaagcc gcccattgag ccggaagtat ccacacagac aataaacggc
    16861 ccgcgcggct gttcatcgta atctttatgt accaccggac gttcgagcac tttttcacgc
    16921 cacgactcac cgtgcaggcg ataggtgagc aactgttttt ccaccagccg acggtaaaac
    16981 tcatactcca gttccgttat ccctagtgtc gccagttctg gcggcaggag acgtaaaata
    17041 tcatcgcttt gttgcagacc atcaacctgc tcaggaaccg tcgccggttc gcgcaccatg
    17101 gtgcggaagg tttccatcta cgcatcgttg cgcggtattg atttggcttc ccgagaacgc
    17161 cccggctgct ctgccaggcg tttcagttcc ggctgttcgt taagaaattc accgtatttc
    17221 acaataaact gatagtcgcc acgtttaagc tgaccggcgc tcatatccca cagacgacca
    17281 gctgcggtat tgttatctgc gagaatcggt tcaagttgtc cgctcagcgt catgcgttcc
    17341 tgaacttcac ttaacagttg ttcgcgttct tcttctaata gctgttgatt taacgtcgtt
    17401 gcttgcacga tcagacttaa acgccaacgc tggagaaaaa gcgtgtgtaa cgctgaagtt
    17461 attgtgctgt tagcatcaac caactgccgg gcttgttctg cccatgggga attcagacga
    17521 tgcagtaagt ctaggatctg tggtagctgc acaataaact gcggcgtgga gaggagctgg
    17581 ctttgctgat agcacatcac ctcttcggtg agttccggcg ggactcgggc atctttcagc
    17641 cgactgcgca gcgcctcacg ccagcgggga acatcatcag tgattgccgc cttcagtcgt
    17701 gggaattttt caaagaagac cgccagctgc ggtgaggcca gcagtgcgat gatcatctct
    17761 tcgatcaatc cctcttcgct gacggccagc atcacattaa gcgtatccag cgttagcatt
    17821 gttgtgcctg gcgaatctgt gcaccgacat cctgtaggct ggcttcaatg cgacctaacc
    17881 agtcgccagg gataaacaga catttttgct gttcgctgaa taaagcgtgt tgcttacgcc
    17941 agtcgctttc aagctcttcc agttgttgtt ttatttcacc tggcagacct tcagccgatg
    18001 aaccgggaag tgccagcgta ctgccttgta aacttacatc gcgtacaaca agatgttggg
    18061 tgctatcaac ttccagattc agtttctggg caaagccgat accgttcagt ttgccgcgaa
    18121 tttcaccacc tttactcagc cactgttcca gcgcgctacg ctcaaaggag atatgaacca
    18181 cttccatatc atgcagtttt aacggttttt gcagcagcag agtcagagtg gaagcagtaa
    18241 cgttaacagg aagttgatac tgctgacgac ggctgaaaat gccgcccaga cgaattaacg
    18301 ttaaggctgt tttatcgctt tgttgctgct ggagttgcag gtgacgttgc acaatcgcgc
    18361 ccaggcgggt caacatccct tgctgttgcc aggcatgacc ggtcatcaat acatcaattt
    18421 gttgttgtat caaattcagg ctttgcgcgt cataccacag gcaatctttc agcaaaatga
    18481 gatcaaccgg ggcaacagca ctgcgaccgc taaaaaaggc gctggcctgc aataagcgga
    18541 tcgctttttt ccagcgacga tccgagacat aaggcgcatc cggtaattta tccagttgct
    18601 ggcgcagcat aaaaatcagc tcaaatacat gatcgggcag cgtaatttca ccaatctctt
    18661 tctgccagcg ttcatattct tcatctgtga cctgcaaggc atcaggaacc ggattgtcgt
    18721 tttcatcctg ttgactggtc agcatggagc ggaaattcgc tttatcttgc actttatcta
    18781 accatagacg aatcagcatg cggtcatata acgcttccag actgctgtct gcttccggca
    18841 gctcgttgga ggccgccacc agtagacgca tcgggatttt ttctacgtgt gcaccgttgc
    18901 ggaactggcg ctcgttaatg gcggtgagca aggtattaag aattgccggg cccgctttcc
    18961 agatctcatc cagaaagacg atttctgctt ccggcaggta accgctggtt aaacgttcat
    19021 agcgcccttc atcttttagc gcctgaatag aaaggggacc aaaaacttct tccggcgtgg
    19081 agaagcgggt catcagatat tcaaacgcgc gggcattctg aaaggcgaat tttaagcgcc
    19141 gggcgatcaa acttttggca atacctggcg ggccaaggag gaacacactt tcaccactta
    19201 atgccgctaa taaacacaag cggatggcgt ggctacgttc ataaagcccc ttttccaacg
    19261 aactgctcag gcgggaaatt ctttccgcta ata
//
LOCUS       DAJTYF010000073        19241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTYF010000073 DAJTYF010000000
VERSION     DAJTYF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.073"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..1865
                     /gene="scpA"
                     /locus_tag="OO590_002629"
     CDS             <1..1865
                     /gene="scpA"
                     /locus_tag="OO590_002629"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6637944.1"
                     /translation="LAAGQKGLSVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDM
                     KVLFDQIPLGKMSVSMTMNGAVLPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCR
                     NTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIE
                     YIKAAISAGLKMDDFAPRLSLFFGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSL
                     ALRTHCQTSGWSLTEQDPYNNVIRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSAR
                     IARNTQIIIQEESELCHTVDPLAGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEA
                     GLPKRMTEEASAREQSLIDQGKRVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLE
                     RIRAARDDAAVTAALNALTHAAQHNENLLAAAVNAARVRATLGEISDALEVAFDRYLV
                     PSQCVTGVIAQSYHQSEKSASEFDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAK
                     VIASAYSDLGFDVDLSPMFSTPEEIARLAVENDVHVAGASSLAAGHKTLIPELVEALK
                     KWGREDICVVAGGVIPPQDYAFLQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            1858..2853
                     /gene="meaB"
                     /locus_tag="OO590_002630"
     CDS             1858..2853
                     /gene="meaB"
                     /locus_tag="OO590_002630"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6637945.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            2864..3649
                     /gene="scpB"
                     /locus_tag="OO590_002631"
     CDS             2864..3649
                     /gene="scpB"
                     /locus_tag="OO590_002631"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6637946.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            3673..5151
                     /gene="scpC"
                     /locus_tag="OO590_002632"
     CDS             3673..5151
                     /gene="scpC"
                     /locus_tag="OO590_002632"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR6637947.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(5093..6043)
                     /locus_tag="OO590_002633"
                     /pseudo
     CDS             complement(5093..6043)
                     /locus_tag="OO590_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6210..6950)
                     /locus_tag="OO590_002634"
     CDS             complement(6210..6950)
                     /locus_tag="OO590_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6637948.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(7043..7678)
                     /gene="argO"
                     /locus_tag="OO590_002635"
     CDS             complement(7043..7678)
                     /gene="argO"
                     /locus_tag="OO590_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6637949.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(7817..8677)
                     /gene="mscS"
                     /locus_tag="OO590_002636"
     CDS             complement(7817..8677)
                     /gene="mscS"
                     /locus_tag="OO590_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6637950.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(8868..9947)
                     /gene="fbaA"
                     /locus_tag="OO590_002637"
     CDS             complement(8868..9947)
                     /gene="fbaA"
                     /locus_tag="OO590_002637"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6637951.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(10162..11325)
                     /gene="pgk"
                     /locus_tag="OO590_002638"
     CDS             complement(10162..11325)
                     /gene="pgk"
                     /locus_tag="OO590_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6637952.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(11375..12394)
                     /gene="epd"
                     /locus_tag="OO590_002639"
     CDS             complement(11375..12394)
                     /gene="epd"
                     /locus_tag="OO590_002639"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6637953.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHSEAHIAAGAKKVLFSHPGSNDLGATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            12766..13197
                     /locus_tag="OO590_002640"
     CDS             12766..13197
                     /locus_tag="OO590_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6637954.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELEFSRQPCALSGYDELHISPVK"
     gene            13220..13798
                     /locus_tag="OO590_002641"
     CDS             13220..13798
                     /locus_tag="OO590_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6637955.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            13799..14107
                     /locus_tag="OO590_002642"
     CDS             13799..14107
                     /locus_tag="OO590_002642"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6637956.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            14123..14506
                     /locus_tag="OO590_002643"
     CDS             14123..14506
                     /locus_tag="OO590_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6637957.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNELAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            14494..15171
                     /locus_tag="OO590_002644"
     CDS             14494..15171
                     /locus_tag="OO590_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6637958.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTCSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            15165..15842
                     /locus_tag="OO590_002645"
     CDS             15165..15842
                     /locus_tag="OO590_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6637959.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(15814..16527)
                     /locus_tag="OO590_002646"
     CDS             complement(15814..16527)
                     /locus_tag="OO590_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6637960.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(16524..17033)
                     /gene="fumE"
                     /locus_tag="OO590_002647"
     CDS             complement(16524..17033)
                     /gene="fumE"
                     /locus_tag="OO590_002647"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6637961.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(17055..18020)
                     /gene="yggF"
                     /locus_tag="OO590_002648"
     CDS             complement(17055..18020)
                     /gene="yggF"
                     /locus_tag="OO590_002648"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6637962.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(18017..>19241)
                     /locus_tag="OO590_002649"
     CDS             complement(18017..>19241)
                     /locus_tag="OO590_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6637963.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
BASE COUNT         4629 a         5172 c         4814 g         4626 t
ORIGIN      
        1 acctggccgc cgggcaaaaa ggtctttccg ttgcgtttga ccttgccacc caccgtggct
       61 acgactccga taacccacgc gtggcgggtg acgtcggcaa agcgggcgtc gctatcgaca
      121 ccgtggaaga tatgaaagtc ctgttcgacc agatcccgct gggtaaaatg tcggtttcga
      181 tgaccatgaa tggcgcagtg ctaccagtac tggcgtttta tatcgtcgcc gcagaagagc
      241 aaggtgttac acctgataaa ctgaccggca ccattcaaaa cgatattctc aaagagtacc
      301 tctgccgcaa cacctatatt tacccaccaa aaccgtcaat gcgcattatc gccgacatca
      361 tcgcctggtg ttccggcaac atgccgcgat ttaataccat cagtatcagc ggttaccaca
      421 tgggggaagc gggtgccaac tgcgtgcagc aggtagcatt tacgctcgcc gatgggattg
      481 agtacatcaa agcagcaatc tctgccggac tgaaaatgga tgacttcgct cctcgcctgt
      541 cgttattctt cggtatcggc atggatctgt ttatgaacgt cgccatgttg cgtgcggcac
      601 gttatttatg gagcgaagcg gtcagtggat ttggcgcaca ggatccgaaa tcactggcgc
      661 tgcgtaccca ctgccagacc tcaggctgga gcctgactga acaggatccg tataacaacg
      721 ttatccgcac caccattgaa gcactggctg cgacgctggg cggtactcag tcactgcata
      781 ccaacgcctt tgacgaagcg cttggtttgc ctaccgattt ctcagcacgc attgcccgca
      841 acacccagat catcatccag gaagaatcag aactctgcca caccgtcgat ccactggccg
      901 gatcctatta cattgagtcg ctgaccgatc aaatcgtcaa acaagccaga gctattatcc
      961 aacagatcga cgaagccggt ggcatggcga aagcgatcga agcaggtctg ccaaaacgaa
     1021 tgaccgaaga ggcctcagcg cgcgaacagt cgctgatcga ccagggcaag cgtgtcatcg
     1081 ttggtgtcaa caagtacaaa ctggatcacg aagacgaaac cgatgtactt gagatcgaca
     1141 acgtgatggt gcgtaacgag caaattgctt cgctggaacg cattcgcgcc gcccgtgatg
     1201 atgccgccgt aaccgccgcg ttgaacgccc tgactcacgc cgcacagcat aacgaaaacc
     1261 tgctggctgc cgctgttaat gccgctcgcg ttcgcgccac cctgggtgaa atttccgatg
     1321 cgctggaagt cgctttcgac cgttatctgg tgccaagcca gtgtgttacc ggcgtgattg
     1381 cgcaaagcta tcatcagtct gagaaatcgg cctccgagtt cgatgccatt gttgcgcaaa
     1441 cggagcagtt ccttgccgac aatggtcgtc gcccgcgcat tctgatcgct aagatgggcc
     1501 aggatggaca cgatcgcggc gcgaaagtga tcgccagcgc ctattccgat ctcggtttcg
     1561 acgtagattt aagcccgatg ttctctacac ctgaagagat cgcccgcctg gccgtagaaa
     1621 acgacgttca cgtagcgggc gcatcctcac tggctgccgg tcataaaacg ctgatcccgg
     1681 aactggtcga agcgctgaaa aaatggggac gcgaagatat ctgcgtggtc gcgggtggcg
     1741 tcattccgcc gcaggattac gccttcctgc aagagcgcgg cgtggcggcg atttatggtc
     1801 caggtacacc tatgctcgac agtgtgcgcg acgtactgaa tctgataagc cagcatcatg
     1861 attaatgaag ccacgctggc agaaagtatt cgccgcttac gtcagggtga gcgtgccacg
     1921 ctcgcccaag ccatgacgct ggtggaaagc cgtcacccac gtcatcaggc gctaagtaca
     1981 cagctgcttg acgctattat gccgttctgt ggtaacgccc tgcgactggg cgttaccggc
     2041 acacccggcg cggggaaaag tacctttctt gaagcctttg gcatgttgtt gattcgtgag
     2101 ggattaaagg ttgctgttat tgcggtcgat cccagcagcc cggtcactgg cggtagcatt
     2161 ctcggggata aaacccgcat gaatgacctg gcgcgtgccg aggcggcgtt tattcgcccg
     2221 gtaccctcct ccggtcatct gggcggtgcc agtcagcgag cacgggaatt aatgctgtta
     2281 tgcgaagcgg cgggttatga cgtagtgatt gtcgaaacgg ttggcgtcgc gcagtcggaa
     2341 acggaagtcg cccgcatggt ggactgtttt atctcgttgc aaattgccgg tggcggcgat
     2401 gatctgcagg gcattaaaaa agggctgatg gaagtggctg atctgctcgt tatcaataaa
     2461 gacgatggcg ataaccatac caatgtcgcc attgcccggc atatgtacga gagtgccctg
     2521 catattctgc gacgtaaata cgacgaatgg cagccacggg gtctgacttg tagcgcactg
     2581 gaaaaacgtg gaatcgatga gatctggcac gccatcatcg acttcaaaac cgcgctaact
     2641 gccagtggtc gtttacaaca agtgcggcaa caacaatcgg tggaatggct gcgtaagcag
     2701 accgaagaag aagtactgaa tcacctgttc gcgaatgaag atttcgatcg ctattaccgc
     2761 cagacgcttt tagcggtcaa aaacaatacg ctctcaccgc gcaccggcct gcggcagctc
     2821 agtgaattta tccagacgca atattttgat taaaggaatt tttatgtctt atcagtatgt
     2881 gaacgttgcc actatcaaca aagtggcggt cattgagttt aactatggac gaaaacttaa
     2941 tgccttaagt aaagtcttta ttgatgatct tatgcaggcg ttaagcgatc tcaaccggcc
     3001 ggaaattcgc tgtatcattt tgcgggcgcc aagtggatcc aaagtcttct ccgcaggtca
     3061 cgatattcac gaactgccgt ctggcggtcg cgatccgctc tcctatgatg atccattgcg
     3121 tcaaatcacc cgcatgatcc aaaaattccc gaaaccgatc atttcgatgg tggaaggtag
     3181 tgtttggggt ggcgcatttg aaatgatcat gagttccgat ctgatcatcg ccgccagtac
     3241 ctcaaccttc tcaatgacgc ctgtaaacct cggcgtcccg tataacctgg tcggcattca
     3301 caacctgacc cgcgacgcgg gcttccacat tgtcaaagag ctgattttta ccgcttcgcc
     3361 aatcaccgcc cagcgcgcgc tggctgtcgg catcctcaac catgttgtgg aagtggaaga
     3421 actggaagat ttcaccttac aaatggcgca ccacatctct gagaaagcgc cgttagccat
     3481 tgccgttatc aaagaagagc tgcgtgtact gggcgaagca cacaccatga actccgatga
     3541 atttgaacgt attcagggga tgcgccgcgc ggtttatgac agcgaagatt accaggaagg
     3601 gatgaacgct ttcctcgaaa aacgtaaacc taatttcgtt ggtcattaat ccctgcgaac
     3661 gaaggagtaa aaatggaaac tcagtggaca aggatgaccg ccgatgaagc ggcagaaatt
     3721 atccagcata acgacatggt ggcatttagc ggctttaccc cagcgggttc gccgaaagcc
     3781 ctacccgccg cgattgcccg cagagctaac gaacagcatg aggccaaaaa gccgtatcaa
     3841 attcgcctgc tgacgggggc gtcaatcagc gccgccgctg acgatgtact ttctgacgcc
     3901 gatgctgttt cctggcgtgc gccatatcaa acatcgtcca gtttacgtaa aaagatcaat
     3961 cagggcgcgg tgagtttcgt tgacctacat ttgagcgaag tggcgcaaat ggtcaattac
     4021 ggtttcttcg gcgacattga tgttgccgtc attgaagcat cggcactggc accggatggt
     4081 cgagtctggt taaccagcgg gatcggtaat gcgccgacct ggctgctgcg agcgaagaaa
     4141 gtgatcattg aacttaatca ctatcacgat ccgcgcgttg cagaactggc agatattgtg
     4201 attcctggcg cgccaccgcg gcgcaatagc gtgtcgatct tccatgcaat ggatcgcgtc
     4261 ggtacccgct atgtgcaaat cgatccgaaa aagattgtcg ccgtcgtgga aaccaacttg
     4321 cccgacgccg gtaatatgct ggataagcaa aatcccatgt gccagcagat tgccgataac
     4381 gtggtcacgt tcttattgca ggaaatggcg catgggcgta ttccgccgga atttctgccg
     4441 ctgcaaagtg gcgtgggcaa tatcaataat gcggtaatgg cgcgtctggg ggaaaacccg
     4501 gaaattcctc cgttcatgat gtattcggaa gtgctacagg aatcggtggt gcatttactg
     4561 gaaaccggca aaatcagcgg ggccagcgcc tccagcctga caatctcggc cgattccctg
     4621 cgcaagattt acgacaatat ggattacttt gccagccgca ttgtgttgcg tccgcaggag
     4681 atttccaata acccggaaat catccgtcgt ctgggcgtca tcgctctgaa cgtcggcctg
     4741 gagtttgata tttacgggca tgcaaattca acgcacgtag ccggggtcga tctgatgaac
     4801 ggcatcggcg gcagcggtga ttttgaacgc aacgcgtatc tgtcgatctt tatggctccg
     4861 tcgattgcta aagaaggcaa gatctcaacc gtcgtgccaa tgtgcagcca tgttgatcac
     4921 agcgaacaca gcgtcaaagt gatcatcacc gaacaaggga tcgccgatct gcgcggtctt
     4981 tccccgcttc aacgcgcccg cactatcatt gataattgtg cacatcctat gtatcgggat
     5041 tatctgcatc gctatctgga aaatgcgccc ggcggacata ttcaccacga tcttagccac
     5101 gtcttcgact tacaccgtaa tttaattgca accggctcga tgctgggtta atggtttgtc
     5161 ccgtcacgat cttcagcaat atgctgaaga tcgtggcggt ataacgatga tttctgattg
     5221 aataaagata ttcctgcata tacatcggcc tgtgcggcgc atcaaaatat ttcagcgttg
     5281 ccggattcac cagtcaccag gcaaaatgcg ggatcaccgt cagaaaatgt ccgcgctcca
     5341 cggcgctgat tttggccata aaactgtagg gacgataaat aatggtggga ttgatcccac
     5401 aaggtcgcat attcgcatca atcattgctt cgaagttagc ccggttttga aagcgcattt
     5461 gtagccaggg gagctcctga agcagctctt gctcttgacg ctctttataa cggcgagaaa
     5521 cgagaaagcc caatcacagc ggtggtaatt cactgatagt caggttttcc agttcctgaa
     5581 ctcgtgcaga tacatgctgt ggggagataa taaaatctaa ctgacggtca aaaagattat
     5641 cgataacgcc gttttcgctg aactcaactg gctgggcggt cacgccttca tacttatctc
     5701 cgagactaat caactgatcg aaaataattg tcggataagt attatctatg ccaatcacaa
     5761 tttctcgtga acgacgccct gagttatgga tttcattatc aatcgcagaa agtcgttgat
     5821 aaaccgggaa cagtttttga tacagttcct gaccggcttt attgaggctg atattattat
     5881 ctttgcgggt aaatagcgta tagccaattt gttcttccag cgcggcaatg cttttaccaa
     5941 atggcgaagc cgtcatatgg attttttctg ccgcccgggc aatattattg gtttgcgcta
     6001 ataaaatgaa attgcgcatc tttttcgaaa taaaaatgtc catcccccct ccccgcatcc
     6061 ttgtccgctt aaccgtttca gtcagcctat ccttgatgaa accgcgagca aagataggtg
     6121 attacgtcat ggttttacag aaaattacag aaaaaggagg caatatcggg taaaggcatt
     6181 agcccgacga atacgtcggg ctacaagtat tattgtgctg caggtgtttt agcgggttgt
     6241 tgatccacag gttctaactg gaagaccaca tcgacctgat catcaaactg aatagcggcc
     6301 tgctcgtaag tttcctgggc agacaccggc gcggcatcgg ctttcatcat ccgcaccatt
     6361 gggctgggct gatagttgga aacatggtag cgcacgctat ataccggccc cagtttacga
     6421 tgaaagccgt tcgccagttc ctgcgcctga tgaatcgcgt tatcaatcgc tgccttacgc
     6481 gctttgtctt tataggcatc cggctgcgcc acgcccagcg acacagaacg aatttcgtta
     6541 agacccgcct tcagcgcgcc atccagcaag gaattcagtt tgtctaactg acggagcgtg
     6601 acttccaccg ttctcacagc tcggtagcct ttcaggatac ttttaccatc ctgatagtca
     6661 taatctggct gggtgcgtaa attcgctgag ctgatatctt ttttcgcgat ctgattgagt
     6721 tcaaggaagg aaatgtattg tgcgacgcgc tcatctgcct gtttcttggc tgtagcggca
     6781 tccttcgcgg ccacgttaac ttcaatcgca agagtggcaa tgtctggcac cgcatccaca
     6841 cttgccgtac cggaggtgac aatatgcggt ccatccggca attcgttagc ctgcgctgcc
     6901 atcccgctaa tacccattaa tgccgccagg gcgataactt tgaacttcac gttagtctct
     6961 ccgtgttaat tgacttgccc aaaattcagg acctctggag gcaagcttag cgcctctgtt
     7021 ttatttttcc atcagatagc gcctaactga acaaggcttg tgcatgagca ataccgtctc
     7081 tcgccagctg caaggctata aaccacatga cacatcccac taccagattg ataatgtgct
     7141 gtgattttgc cgtgcgcagg cgcggtgcca gccaggctgc gagaatagcc agaccaaaga
     7201 accacaggaa agaggcgcta attgtcccga gcgcaaacca acgttttggt tcgacgtcaa
     7261 gctgcccgcc aaggctgccc agtacaacaa aagtatccag gtaaacatgc ggattcagcc
     7321 aggtcactgc caacatggtg gcgataattt tccatctgcc ttgcttcagg acttcggcac
     7381 tagctaactc aatattacta ctcatcgctg ttttaaaagc gccaaaacca taccagagca
     7441 agaagactac gccgccccaa gtgaccagcg ctagcaacca cggcgactgc atcaataacg
     7501 cgctaccacc aaaaatcccc gcgcaaatca ggaccaaatc gctgatagcg caaagtaagg
     7561 caatcataat gtggtactga cgacgaatgc cctgattcat cacaaaagca ttttgtgggc
     7621 cgagcggcag aatcatagct gccccaagag caagaccttg aaagtaataa gaaaacacgt
     7681 tggatattcc gaattaagta tctgatgcag agactatatc gcgggattgt tattagcgga
     7741 aattgataat attaattggc aatcagaata actaataaga gagaggccag agagtattat
     7801 ctggcctcag cgttgattac gcagctttgt cttctttcac ccgcttaaag ttcacatcca
     7861 tttgcgggta cgggaagctg ataccggcgg catcaaattc acgtttaata cgctccagca
     7921 catcccagta cacgttttgc agatcgccgc tgttgctcca gacgcggacc acgaaattaa
     7981 tcgacgatgc accaagttcg ttcaggcgca cagtcatttc gcgatctttc aaaatgcgat
     8041 cttcagactg gataatattg gtcaggatct gcttaacctg atcgatatcg gaatcatacg
     8101 ccacgccaat aataaattcg ttacggcgaa ctggctcgcg ggagaagtta ataatatttc
     8161 cggcaataat tttaccgttc ggaataacga taattttacc gtctgcagta cgcatggtgg
     8221 tggagaaaat ctgcacactc agcacagtac cggctacgcc gccgaggtca acatattctc
     8281 cggcacggaa cgggcggaac atgacaagta acacgccagc ggccaggtta gaaagtgacc
     8341 cctgcaaagc cagaccaaca gctaagcctg cggcaccgag tacagcaatg actgacgctg
     8401 tttgtacacc cacgcgtccc agggcagcga ttagcgtaaa ggcgataata ccgtaacgga
     8461 ctaatgcaga aagaaaatca gcaacagtgg catcgatttt acgggagatc atcaggcgat
     8521 tcaccgcgtt ggaaatcatc cgcgcgataa tcaaaccaac gatgatgatc gcgagtgccg
     8581 ccacgatgtt tactgcataa cttagcagca gcgcctggtt agctaccagc cagcttcccg
     8641 cgccgtttat gctatcgaca acattcaaat cttccattca atattccttt tcaactgact
     8701 caaaatggag aaatacactc cgccgcctta tgacgggcag tctgacaagg gtaaacaata
     8761 aagcataatt ttgccaaata gatcacagat ttctcatctg aatcaccttg cttttggcga
     8821 aaaaaagacc cgcagagcgg gccttgagat aagcagaaag gaatgactta cagaacgtcg
     8881 atcgcgttca gttcctggaa tgctttttcc agacgagcga tcatcgaagt ctgaccggca
     8941 cgcagccata cgcgcggatc gtagtatttc ttgttcggct gatcttcgcc tttcgggtta
     9001 cccagctgac cctgcagata agcttcgttc gctttgtagt agttcagaac gccttcccag
     9061 gttgcccatt gggtatcggt atcgatgttc attttcacta cgccgtagct tacggagtct
     9121 ttgatttcct gagcagtaga accggaacca ccgtggaata cgaagttcag gctgttgtgc
     9181 ggcaggttgt gtttcttaga aacatattcc tgagaatcac gcaggatggt cggagtcaga
     9241 accacgttac ccggcttgta aacaccgtgt acgttaccga aggacgcagc gatggtgaaa
     9301 cgcgggctga ttttgctcag ctcggtgtat gcgtaatcaa cgtcttccgg ctgggtgtac
     9361 agtgcagaag cgtccatgtg gctgttgtcc acgccgtctt cttcaccacc ggtgcaaccc
     9421 agttcgattt ccagagtcat gccgattttg gacatacgct ccaggtattt agagcagatt
     9481 tcgatgttct cttgcagaga ttcttcagac aggtcgatca tgtgagaaga gaacagcggc
     9541 ttaccggtag ctgcgaagtg tttttcaccc gcgtccagca gaccgtcgat ccacggcagc
     9601 agtttcttcg cgcagtggtc agtgtgcagg ataaccggaa caccataatg ttcagccatc
     9661 tggtgaacgt gatgcgcacc agagatcgcg cccaggatag cagcaccctg cggaacgtca
     9721 gatttcacgc ctttaccagc gataaaggaa gcaccaccgt tggagaactg aacgataacc
     9781 ggcgctttaa ctttagcagc agtttccagt acggcgttga tggagtcagt accgacgcag
     9841 tttactgctg gcagtgcgaa gttgttttct tttgctacct ggaaaacttt ctgtacgtca
     9901 tcaccagtga ttacgccagg ttttacgaaa tcaaaaatct tagacatgtc tcttgtcctg
     9961 tatcgtcggg ccttatactc ataatacttc aagttgcatg tgctgcgtct gcgttcgctc
    10021 accccagtca cttacttatg taagctcctg gggattcact ctcttgtcgc cttcctgcaa
    10081 ctcgaattat ttagagtatg aaaaaatgcg tgctctaaaa gcgcgctgaa acaagggcag
    10141 gtttccctgc cctgtgattt tttacttctt agcgcgctct tcgagcatcg ctactgcagg
    10201 cagtacttta ccttccacga attcgaggaa tgcgccgccg ccagtggaga tgtaggagat
    10261 tttgtcagca atgccgaaca ggtcgattgc tgccagagtg tcgccgccgc cagcgatgga
    10321 gaacgcttcg ctgtctgcga tagcgttagc cacgatttca gtacctttgc ggaagttcgg
    10381 gaattcgaac acgccaaccg gaccgttcca cagaatggtt ttcgcattct tcaggatttc
    10441 agccagttcc tgagcggaag catcaccgat atccaggatc tgctcgtcag ctttcacatc
    10501 gttaacagat ttcagggtag ccggtgcagt ttcagagaac tcggttgcta cgcgaacatc
    10561 agacggaacc gggatgttgc aggtggtcag cagacgttta gcttcgtcaa ccaggtcagc
    10621 ttcgtacagg gatttaccca catcgtggcc ttgtgccgcg ataaaggtgt tagcgatacc
    10681 accaccaaca atcagctggt cagcgatttt agacagggag tccagaacgg tcagtttggt
    10741 agatacttta gaaccaccaa cgatagccac catcgggcga gcaggttctt tcagtgcttt
    10801 acccagcgcg tccagttcag ctgccagcag cgggcctgcg cacgcaacgt cagcgaattt
    10861 accgataccg tgagtagaag cctgcgcgcg gtgagcagta ccgaatgcgt ccattacgaa
    10921 cacgtcacac agtgcagcgt attttttgga cagggtttcg tcgtctttct tctcgccttt
    10981 gttgaagcga acgttttcca gaacaaccag ttcaccttca gcaacgtcaa cgccgtcgag
    11041 gtaatcttta accagacgaa ccgggttaga cagtttgtct ttcaggtagt taacaaccgg
    11101 cagcagagag aattcttcgt tgtactcgcc ttcggtagga cgacccaggt gggaagttac
    11161 catcactttt gcgccttgtt tcagggccag ttcgatggtc ggcagagaag cacggatacg
    11221 cgcgtcgctg gttactttcc cgtcttttac tggtacgttc agatccgcac ggataaatac
    11281 acgtttccca gcaagatcca gatcggtcat cttaattaca gacatggtga atcctctcgt
    11341 tgattctaaa agttttgcag acgctgcttg cgtcttacct gaaagcaaca gtagccatag
    11401 ctaacgtcgt gtcgagcatt cggttagcaa agccccattc gttatcgcac cagaccaacg
    11461 ttttgatcag gtgtgcgcca ctgacccggg tttgggtgcc atcgacaatg gcactgtgcg
    11521 gatcgtggtt aaaatctaca gagaccaacg gcaattccgt atagtcaact ataccatgaa
    11581 atgcaccttg tgctgctttt tgcagcaaca ggttgacttc attggctttt acaggtttct
    11641 tcaccgtcac gcttaaatcg attgccgtca catttatggt tggcacacgt accgcaatcg
    11701 cttcaaagcg atcgttaaat tgcggaaaaa atcgtgtgat accagcggcc agtttagtat
    11761 cgaccggaat gatcgactgg ctggctgccc gggtgcgacg caggtcagga tggtatgcat
    11821 caataacctg ttgatcgtgc atggcggagt gaattgtggt cacagtgccg gactcaatac
    11881 cgtacgcatc atctaacaat ttgatgacgg gaattatgca attcgtggta caggaagcgt
    11941 tagaaacgat gcggtgttcc gcacgaagtt gatcctgatt gacgccgtaa acaacggtcg
    12001 cgccgagatc gttactgcca ggatgtgaaa agagcacttt tttggccccg gcggcaatat
    12061 gcgcttcgct atgctcgcgg gagccatata cgccggtgca gtcgaggact acatcaacgc
    12121 caagttcacg ccaggggagc gattgcagtg aacgttcatg caatacgcgg atggcgtcat
    12181 caccaacaaa aagttgatcg cgttcctgtc gtacttccca tgcaaaacgg ccatggctgg
    12241 tgtcatattt caacaaatgc gccatgcccg cagcatccgc cagttcgttg attgccacca
    12301 cggtaatttc cgcccggcgt ccggattcat acaaagcacg aaccacatta cgcccgatgc
    12361 gaccgaagcc atttatcgct acgcgtacgg tcatagatct cctgcaaggt tttccctgag
    12421 caaatttgcc agacagagta atctagcaaa tcgtccgggg aaaccttacc tgtcgcaaac
    12481 tgcgactgat tggttaattg tcgaacattt aatcgactga aacgcttcag ctaggataag
    12541 cgaaacgtgg aataaaagga atgattgtcc agtcaaagaa agcaattatc tgacttacgt
    12601 cacgttttca gccccgcccc tgtggaattt attccacttt gcagaatcac gaataagcgt
    12661 agcaaaatct tttcgttgtg ccgtcacaaa ccctgactta tatcagaata tggctgatgc
    12721 agcgtcgata ttatccgcaa tacgtccgag tttcggagta ctccaatgat gacattgccc
    12781 tacccatatt cttcctccgc acgcttttac gcactgcgcc tcctccccgg gcaggaagtt
    12841 ttttcccagc tacacgcttt tgcacagcaa catcaacttc atgccgcctg gattgcaggc
    12901 tgtaccggta gcctgactga cgtcgccttg cgctatgccg ggcaagaagg caccacgctc
    12961 ctgaacggta cattcgaagt gatctcacta aacggcacgc tggaacagag tggtgagcat
    13021 cttcatctct gcgtttccga tccacacggc acgatgcttg gcggtcatat gatgcccggc
    13081 tgtaccgtgc ggaccacgct tgaactggtg atcggtagcc ttgaggaact ggagtttagc
    13141 cgccaacctt gcgcgctttc tggttacgac gagttgcata tttcccccgt taaataacta
    13201 ctttcaaaga ggttttctta tggcgcgtag ccacttttcc agtcaggcac tggtgttgat
    13261 cgttatatct atcgccatca atatgatcgg cgggcaactg gccagcatgg tcaaattacc
    13321 tatatttctc gactccatcg gcacacttat tagcgcggta ctgcttggtc cggtgatcgg
    13381 aatgctgacc ggcttactga ccaatttact ctggggattg ctgaccgacc cgatcgccgc
    13441 cgcatttgca cccgtcgcga tggtcatcgg cctggtctcc ggttggctgg cgcgagcggg
    13501 ttggtttcgc accctgccaa aagtcgttgt cagcggcgtg attattacgc ttgcggtaac
    13561 ggtcgtcgct gtaccgctgc gtactgcgct gttcggcggt gtcaccggta gcggagccga
    13621 tttgttcgtt gcctggatgc actcgatggg gcaaaacctg gtggagtcgg tggcgattac
    13681 ggtgataggc gcgaacctgg tcgataaaat tcttacagcg gtgattgtct ggcttttact
    13741 gcgccagctg ccgattcgca ccacgcgcca ttttccggca atggctgccg tgcgctaaat
    13801 gcatccattt acctcattaa cactgtgggc attagcggcc tgcaccacgc ttatcctgcc
    13861 cgcgcagacc attttgccga tctatagcgc ggcgaccttt ttctgcctta ttgcgctaaa
    13921 ggccacgcgg cggcgggcaa aatatgtcgt ctggctgatg ttctcgctgg gagccgggct
    13981 gtggctggta cacggtggct ggctaccaga atggctaagc ggcacgccac gctcgccaga
    14041 acggtggtcg catgctatta cgctctggtt gctgattctg gcgattgttt caacctcaca
    14101 gttgtgaatg cagtacgtcc ccgtgcaatg ttttattcgc gctctgtttg cttctcgcct
    14161 gccgccaggc gtcgcttatc tgtttgccgg gccgttattg gtcgttgagc agttaaaacg
    14221 gcaattagcg attattcatg aagcgcagcg tgcccgcggc gtgccgctgg atgagggctg
    14281 gtatcagcgt ttacgtgcta tgcccgcgtt aattattccg ctaacacaca atgcgcttaa
    14341 cgagctggct gtccgtggtg cggcgctgga tatgcgggca tttcggatcc acaaccgacg
    14401 aacaacgttg tgggcaccag ccgatagcac cctacaacgc gtagcgcgtt acaccatgat
    14461 cttgctgatg ctgacagaat tcggagcgtg gatatggtta cgttagaaca gttccgttat
    14521 tgcccgacac actcaacgca tcctccgttc tgttatgact ttcactatgt caaaccgggg
    14581 atggtcgcca tctttggtga caacggcagt ggcaaaagta cgctggcaca gctgatggcg
    14641 ggctggtatc cggactttct gccaagagag ataacaggca cggggacgct cctcggtacg
    14701 cccattgggc atctcccgct taatgaacaa tccgccacca tccagctcgt ccagcaatcg
    14761 ccctatttgc agctctccgg ctgtacctgt agcgtggaag aagaagttgc ctttgggcca
    14821 gaaaatctgt gtcttgctga agcagagatt atggcgcgga ttgatgccgc cctgactctg
    14881 accgagtgcc agccgttgcg ccatcgtcac ccggcaacac tctccggtgg agagacacag
    14941 cgcgtggtca tcgcctgcgc catcgcgatg cagccaaagc tcttaattct tgatgaagcc
    15001 tttagccgcc tgacgccgca ggccagcgaa atgctgttgc agcgcttaca acactgggcg
    15061 tttgaacgtg gttcgctgat tattcttttt gaacgtcatc gcacaccttt cctgaactac
    15121 tgccagcaag cgtggcaatt gcaaaacgga gcgctccagc cactatgtta acgttaaata
    15181 agatatctta tcgctggcct ggcgcggcaa cggattgtct ttgcgacatt tctctgcaac
    15241 tcaaacaagg tgaatggttg gcgctgaccg gtgacaatgg cgcagggaaa tcgacgctgt
    15301 tacgagtaat ggcaggtctt cttacgccca cagccggtac ggttatgctg caacaacagg
    15361 cgatgaaaaa cctgaaaaac cgccagcgcg cggcaaaggt tggcgtactg tttcaggaag
    15421 cggaaaacca actttttcac agcaccgtcg ctgacgagat cgcatttggt ttgaaactgc
    15481 aaaaatgccc ggcggatgaa atcactcaac gtacccatgc tgccctgcaa tgttgtcagt
    15541 tagcagacac tgccaactcg cacccgttgg atctgcatag tgcgcagcga cggatggtcg
    15601 ccgtcgcctg cctggaagca ctctctccgc cactattact gctggacgag ccgagtcggg
    15661 attttgacga aaattggttg agcgtgtttg aaagctggct ggagaagtgc cgccagcgag
    15721 gcacgagcgt ggtagcaatc agtcatgacg ccgcatttac ccggcgtcat ttctctcgtg
    15781 tggtgcgact ggaagatggt gtaatcagga acgttaatcc accagacgat attcacccgt
    15841 agcggtcatc attaaggtca aatttgcagg cagactctct tccagcaccc ggcgaacatt
    15901 cggcccgtcg gtacggtcat aaaaggcttc tgcatctgcc agcgataccc ctcccgccag
    15961 cttgcgcccc accagacgct cccgcaacgc agcagctggc gcgttgataa agatcgagaa
    16021 atcacaaaat tgcgcaagct ggcaccactt ctcgtcgtcc agcaacaacc agtttccttc
    16081 gacgatgacg agtggtgcgg taacgtgcaa cgcatcttca acaggatcat gcttttgtcg
    16141 atcgtactgc ggccacgtac aatccccttc caccacctgg cgcagatttt ccgccagttt
    16201 cgcaacgtca aatgtctctg gtgcgccttt gaaggggcgc aattgatgcg cgtccagcca
    16261 gctattgtaa tggtgaaaac catccatcgg gagcgtctga atagcgggca gctccgggtc
    16321 ttgttgcgcg agatattccc agaaggtggt tagggtagat ttacccgtcc ccggcggagc
    16381 gcaaagaaaa accaccgtac gccgctgcgg atttaccgtc tgtaaggccg ccagcatacg
    16441 cagcaacggt ttatgcacgc gttctatctc ttcatcatgg tactgcgctt gcacgttcag
    16501 cccattcacc gttaattcaa ttttcacggc gttaattcct ttaaggtggt cgttactgcc
    16561 agcgtcagcg ccgttttgac cattccgcta aaacgcgcct ctgaccagac ggcgaaatcg
    16621 gcgaacttca tcgcttttaa cgcatcgtag agcgtcgcat tgcgagttat actgttgaca
    16681 ttgctgataa aatcccaggt tatatcatcg gcaaatccgg gcgtttcatc ctgttcgttc
    16741 aggtaatgcc agtactggga aaagtgcgtg atatcggcat acagccattt atccattttc
    16801 cccagcgcat aaatcaaacg cagcgcaaca tcaatatcgt caagcgggcc gctttgggcg
    16861 agtaacggct tcactgcata ttcgacgatc tcttcatcgt tatcgacaaa cagcgacggc
    16921 agaaactggc gtatcccctg gagcaaaata gaatgcgcag ttttcataaa tgaaaataaa
    16981 ttgtcctgtg catccagttg ctcaagcaca tcatcttctg tcagagtcgc cattggtctt
    17041 ccgatagatc accatcaatg cagggagtct attatattac acgtttggtc cgcgccgccg
    17101 atcagtaatg tctgcgtccg caccccattc gccgtctgtt ggataccgtt caccaactcg
    17161 ccgcccgtca cgcccgtagc gctaaagagg atatcgttcc ccctcaccag ttcatcgagc
    17221 gagtacacac ggttaacgtc aacgcccatt gccttacaac gcttgcgctc ctgctctgca
    17281 atctgccgat tttccgtgta atccccttta gcctgacaaa agtcaatcag ctcggcctgc
    17341 atatccccgc ctaacgcttt aacggcacag gcagaaatca cgccttctgg cgcaccgccg
    17401 atggtgtaca tcacatcgta aggattatcc tgccagcagg tcagcacgct ggcggcgaca
    17461 tcgccatccg gcagggcaaa aaccttcacg cccagctgtg ttgcttcttc aatcgcggca
    17521 ctcagacgcg gtttatccag cgtaaccatg cgcagcttgt ccagcggttt acctaacgcc
    17581 cgcgccacgt tacgcaagtt gtccgccaga ggcaaagaca gatcaatcgc cccggcagcc
    17641 agtcgattaa caaccagctt tttcatatac atatcgggcg catgtaacag gctatctcga
    17701 ggagcgaaag ccatgaccgc cagcgcattg ctctgcccca tcgccaccat ccgcgtgcct
    17761 tcaatgggat caaccgcgat atcgacttct ggcccatcgc ctttgcccac ctcttcgcca
    17821 atccacagca tcggcgcatg gtcaatttct ccttcgcaga taaccactcg tccacgaaaa
    17881 gcgacgtcgt ttaatgcctg gcgcatcgcg gtgaccgcca ggccatcaat tttatttttg
    17941 tcgccacatc cggtttgcgg ccaggcagcc aacgcggcct gttccgtaac gcgaaataat
    18001 ggccacgcca gggacatcat tgcgcggcct ccccgatatc aacgccaaat tgtgccagca
    18061 gatatttctc ggcctgttca ttccagatgc cgtgcgtttc ctcaaccagc cgcgccaact
    18121 ctttaaacag cggatcggtt ttgccttttt cggcaaaatc ggcaatggca gtgagcggca
    18181 tggtcacgcc gttataaatg agttttttac cgccagggat atccggcaga ttgagcacgg
    18241 tttctggcac cgcatccagg ccaccaatat gggtcaccat aaacgacggc tgtaactgtc
    18301 ctgtggcgct aagggcaatc gcctctttca tgtcgtccgt tgaaccgccg gatgtaccga
    18361 ctacgtgcgt gctgttgtaa tggacgttgt agaaattaaa cggcactttg aagtttttat
    18421 ccgtcagccc ggcaaagaag ttcagacagc catcttccgc cagtaattcg tcagccatct
    18481 caacgacagc aggcaccgcc gcataaacaa aaatgtcatc gaacccgaca tctcctgtca
    18541 gcgcccgcag catctggata ggatcgctca tccctttggt attcacatac accagctcaa
    18601 tgcctttact ggccgccaga tccaccggca gcagcttctg cacctgcgcc aggcgtttgt
    18661 cgtcgatatc gaccaccacc acccgtgacg gttgtatgcc accgttaatg gcgtaatcga
    18721 tagcgccaat gcccatcgga cccgcacacg ccagcagtgc aatattgccg ccaggtttga
    18781 cgcccatgcg atgctcataa acatattgcg tggtgtgata attggcatga taagcaccaa
    18841 taatgcagca cataggctct gccagcgacg ccgcagcaaa ataagagccg tgatacggta
    18901 atacgcagcc caaattaatg gcgatttcgg gaataatcat ataagtggca ttgccgccaa
    18961 aatattcgta gctgtagccc gctgaatatc cgcttggtaa ccccatcgcc ggttgcaata
    19021 caaaacgctg gcctttttta tatttgccag tgagattttt acccacttcg acaataatcc
    19081 cggcacattc atgcccggta atgaccggat gattttctaa atcgtcgggt acgcgtttat
    19141 gttcactacc gagtaacgcc gctttccagg tcgataaaca gacgctgtca gaaattacac
    19201 tcactaacag ttcattatcg gtaatttctg gcagttcaaa t
//
LOCUS       DAJTYF010000074        17901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTYF010000074 DAJTYF010000000
VERSION     DAJTYF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.074"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO590_002650"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO590_002650"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6637964.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO590_002651"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO590_002651"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6637965.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..2999)
                     /locus_tag="OO590_002652"
     CDS             complement(1728..2999)
                     /locus_tag="OO590_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6637966.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3182..4386)
                     /locus_tag="OO590_002653"
                     /pseudo
     CDS             complement(3182..4386)
                     /locus_tag="OO590_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4486..4842)
                     /locus_tag="OO590_002654"
                     /pseudo
     CDS             complement(4486..4842)
                     /locus_tag="OO590_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4978..5085)
                     /gene="rprA"
                     /locus_tag="OO590_002655"
     ncRNA           complement(4978..5085)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO590_002655"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="OO590_002656"
     CDS             complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="OO590_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6637967.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAILAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6772..9828
                     /locus_tag="OO590_002657"
     CDS             6772..9828
                     /locus_tag="OO590_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6637968.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVVLIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9825..10235
                     /gene="menI"
                     /locus_tag="OO590_002658"
     CDS             9825..10235
                     /gene="menI"
                     /locus_tag="OO590_002658"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6637969.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10335..10523
                     /locus_tag="OO590_002659"
     CDS             10335..10523
                     /locus_tag="OO590_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6637970.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11071..11439
                     /gene="sufA"
                     /locus_tag="OO590_002660"
     CDS             11071..11439
                     /gene="sufA"
                     /locus_tag="OO590_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6637971.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11448..12935
                     /gene="sufB"
                     /locus_tag="OO590_002661"
     CDS             11448..12935
                     /gene="sufB"
                     /locus_tag="OO590_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6637972.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12945..13691
                     /gene="sufC"
                     /locus_tag="OO590_002662"
     CDS             12945..13691
                     /gene="sufC"
                     /locus_tag="OO590_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6637973.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13666..14937
                     /gene="sufD"
                     /locus_tag="OO590_002663"
     CDS             13666..14937
                     /gene="sufD"
                     /locus_tag="OO590_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6637974.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14934..16154
                     /gene="sufS"
                     /locus_tag="OO590_002664"
     CDS             14934..16154
                     /gene="sufS"
                     /locus_tag="OO590_002664"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6637975.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQAQGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16167..16583
                     /gene="sufE"
                     /locus_tag="OO590_002665"
     CDS             16167..16583
                     /gene="sufE"
                     /locus_tag="OO590_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6637976.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16732..17736
                     /gene="ldtE"
                     /locus_tag="OO590_002666"
     CDS             16732..17736
                     /gene="ldtE"
                     /locus_tag="OO590_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6637977.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17800..>17901)
                     /gene="lpp"
                     /locus_tag="OO590_002667"
     CDS             complement(17800..>17901)
                     /gene="lpp"
                     /locus_tag="OO590_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6637978.1"
                     /translation="NDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4699 a         4339 c         4612 g         4251 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccagccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcttgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtaaccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaacggt ttgctgttca tttgcggtaa ttcattagct
     2401 acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt
     2461 acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata
     2521 tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg
     2581 accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc
     2641 agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg
     2701 caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc
     2761 cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa
     2821 ccgataccgg aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgccaga
     2881 gatgtcatat tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa
     2941 atacaaagaa cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt
     3001 gttctccgac ggtttacata attactatac tgaagatgat tttccatggg tacatggcat
     3061 aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt
     3121 agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc
     3181 attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc
     3241 gtaagccgat aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg
     3301 caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc
     3361 ccgcactgta atagatccct gtagctttac ctttagcgta agggaaacgt tcagctatta
     3421 acgtcaggcc aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata
     3481 ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa
     3541 aggtgtacag catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa
     3601 taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt
     3661 atgacatgcc tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg
     3721 ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat
     3781 gttccgtaga gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga
     3841 acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg
     3901 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
     3961 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
     4021 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa
     4081 agccaaaaac ataagcgata atgatgttat tggtctgcag gatgccaaaa aagaaggcca
     4141 tatagcagca cattccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
     4201 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
     4261 ccgcggcatt agtctgacaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
     4321 cgcccatacc atgcaccagg taataaaata caacccaagt gcggtagggt aatagggatt
     4381 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4441 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4501 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4561 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4621 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttactgacg
     4681 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4741 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4801 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4861 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4921 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4981 aaaaaaagcc catcgtggga gatgggcaaa gactacacac agcaattcgt tgtttcactc
     5041 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     5101 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     5161 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     5221 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     5281 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggcggcggg
     5341 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5401 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5461 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5521 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5581 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5641 attaacaccg ttagcagcgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5701 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgag gatagcctgt
     5761 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5821 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5881 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca agtggtggtg
     5941 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     6001 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     6061 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     6121 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     6181 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     6241 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     6301 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6361 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6421 cgcgacgtgg acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6481 aggccgattc tgtccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6541 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6601 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6661 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6721 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6781 cagatttccc aggcacccgg cgtcgttcaa ctggtgctta attttttgca agagctggag
     6841 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6901 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6961 gtggtgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     7021 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     7081 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     7141 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     7201 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     7261 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     7321 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7381 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7441 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7501 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7561 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7621 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7681 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7741 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     7801 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7861 cgagttaatg cactctgtgt gcggcttgat gagctgattg ctagccaaca ggcaggtgtg
     7921 attggctggc aggtgtgccg cgagctggcg ggggttgaac gtatctatgc gatgcgcaaa
     7981 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     8041 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     8101 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     8161 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     8221 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     8281 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     8341 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8401 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8461 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8521 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8581 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8641 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8701 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8761 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8821 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8881 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8941 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     9001 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     9061 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     9121 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     9181 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     9241 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     9301 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9361 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9421 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9481 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9541 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9601 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9661 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9721 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9781 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9841 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9901 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9961 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
    10021 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
    10081 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
    10141 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    10201 gctgttcgtc acgattgacg accgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    10261 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    10321 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10381 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10441 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    10501 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10561 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10621 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10681 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    10741 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10801 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10861 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10921 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag catgttttac
    10981 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    11041 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    11101 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    11161 ctggtggcaa agcagccagg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    11221 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    11281 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    11341 gaaatcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11401 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11461 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    11521 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11581 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11641 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11701 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11761 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11821 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11881 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11941 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    12001 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    12061 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    12121 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    12181 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    12241 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    12301 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12361 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12421 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12481 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12541 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12601 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12661 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12721 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12781 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12841 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12901 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12961 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    13021 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtact ttatcggcaa
    13081 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    13141 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    13201 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    13261 tgcgcagcta tcgcggccag gaaacgcacg accgctttga ttttcaggat ttgatggaag
    13321 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13381 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13441 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13501 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13561 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13621 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13681 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13741 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13801 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13861 gcgggagata tatccccaca gcagcgtgat gccttagcgt taacgttaga cgccgtgcgg
    13921 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13981 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    14041 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    14101 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    14161 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    14221 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    14281 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    14341 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14401 ttcctgctgg gtggcgcagt gttacgacac aacaccagta cgcaactcaa tggcgaaaac
    14461 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14521 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14581 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14641 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14701 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14761 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14821 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14881 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14941 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    15001 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    15061 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    15121 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    15181 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    15241 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    15301 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15361 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15421 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15481 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15541 aggcggtgat gcatcatccg gtggatgttc aggcgcaggg ttgcgacttt tacgtgttct
    15601 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15661 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15721 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15781 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15841 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15901 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15961 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    16021 actgcgctat gccattaatg gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    16081 ccatgtataa cacccatgaa gaagtggatc gtctggtgac cggcctgcaa cgtattcacc
    16141 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    16201 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    16261 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    16321 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16381 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16441 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16501 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16561 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16621 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    16681 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16741 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16801 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16861 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcggcaatg
    16921 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16981 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    17041 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    17101 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    17161 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    17221 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
    17281 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    17341 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17401 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17461 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17521 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17581 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17641 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17701 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaaat
    17761 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    17821 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    17881 acgcattgcg ttcacgtcgt t
//
LOCUS       DAJTYF010000075        17572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTYF010000075 DAJTYF010000000
VERSION     DAJTYF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.075"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(267..1295)
                     /gene="epmB"
                     /locus_tag="OO590_002668"
     CDS             complement(267..1295)
                     /gene="epmB"
                     /locus_tag="OO590_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6637979.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1337..1903
                     /gene="efp"
                     /locus_tag="OO590_002669"
     CDS             1337..1903
                     /gene="efp"
                     /locus_tag="OO590_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6637980.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1955..2080
                     /gene="ecnA"
                     /locus_tag="OO590_002670"
     CDS             1955..2080
                     /gene="ecnA"
                     /locus_tag="OO590_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6637981.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            2191..2337
                     /gene="ecnB"
                     /locus_tag="OO590_002671"
     CDS             2191..2337
                     /gene="ecnB"
                     /locus_tag="OO590_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6637982.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2513..2742
                     /locus_tag="OO590_002672"
                     /pseudo
     CDS             2513..2742
                     /locus_tag="OO590_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2739..3272)
                     /gene="blc"
                     /locus_tag="OO590_002673"
     CDS             complement(2739..3272)
                     /gene="blc"
                     /locus_tag="OO590_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6637983.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3361..4494)
                     /locus_tag="OO590_002674"
     CDS             complement(3361..4494)
                     /locus_tag="OO590_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6637984.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="OO590_002675"
     CDS             complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="OO590_002675"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6637985.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="OO590_002676"
     CDS             complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="OO590_002676"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6637986.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5333..6067)
                     /gene="frdB"
                     /locus_tag="OO590_002677"
     CDS             complement(5333..6067)
                     /gene="frdB"
                     /locus_tag="OO590_002677"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6637987.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="OO590_002678"
     CDS             complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="OO590_002678"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6637988.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            8193..9170
                     /gene="epmA"
                     /locus_tag="OO590_002679"
     CDS             8193..9170
                     /gene="epmA"
                     /locus_tag="OO590_002679"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6637989.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9389..10891
                     /gene="yjeM"
                     /locus_tag="OO590_002680"
     CDS             9389..10891
                     /gene="yjeM"
                     /locus_tag="OO590_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR6637990.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="OO590_002681"
     CDS             complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="OO590_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6637991.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSIAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(14375..15343)
                     /gene="psd"
                     /locus_tag="OO590_002682"
     CDS             complement(14375..15343)
                     /gene="psd"
                     /locus_tag="OO590_002682"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6637992.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="OO590_002683"
     CDS             complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="OO590_002683"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6637993.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNIKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDG"
     gene            16587..17132
                     /gene="orn"
                     /locus_tag="OO590_002684"
     CDS             16587..17132
                     /gene="orn"
                     /locus_tag="OO590_002684"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6637994.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17343..17418
                     /locus_tag="OO590_002685"
     tRNA            17343..17418
                     /locus_tag="OO590_002685"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17376..17378,aa:Gly,seq:gcc)
BASE COUNT         4070 a         4520 c         4612 g         4370 t
ORIGIN      
        1 aaaggttaat tattggtgtt agctatataa aaagtaacaa cagcaatgca tatgaataat
       61 taaaggttat gcaacgtgca aagattaaat ttctcagttt aatgacaggt tgctgtactt
      121 gcgtttgttg ctcttgccgg atgcggcgta aacgccttat ccggcctaca gttcattgca
      181 aatccattca atttcaatgc acgatgtagg cctgataagc gtagcgcatc aggcaatttt
      241 aatgtttaac ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt
      301 ttgctgggtt cgccgccaat ttctcgcgcc agtttcggca ccagatatcc cgacaccaat
      361 gtcagcaact cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg
      421 ccctgtactt tatctagcac atgcaggtaa tacggcatca cgccggcatc gaacaacgca
      481 ttactcaggt ttgccaacgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg
      541 ttaagcaggg tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct
      601 acctcattgg catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag
      661 cgttcaacca gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc
      721 cgcagacgtt tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga
      781 tctttcgcca tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca
      841 gcaacatact caagtgcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg
      901 aagtggcgac ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc
      961 gcccggttgt ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagtggg
     1021 tcggtggaga atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa
     1081 agaggatcgt ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg
     1141 gcaaacagct ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa
     1201 agacgcagaa gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct
     1261 ctggatgggg tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca
     1321 aatttcagag ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat
     1381 catgttagac ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg
     1441 ccaggcattt gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac
     1501 cttcaaatct actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct
     1561 gtacaacgac ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc
     1621 tgatgcaaaa gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt
     1681 aactctgtgg aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat
     1741 cgttgatacc gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac
     1801 cctgtctact ggcgctgtgg ttaaagttcc gctgttcgta caaatcggcg aagtcatcaa
     1861 agtggatacc cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc
     1921 ctgcaggctg caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt
     1981 tttgcttgcc agcacgctgc tcacgggctg taacaccgct tgcggtttcg gcgaagacat
     2041 caaacatctc ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa
     2101 atcatcagat ttccatcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac
     2161 ataaataaca ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt
     2221 tctgttctgg tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa
     2281 gacatttctg atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaataagca
     2341 ataacggtac gacagcagtg tcgtgccgtt tgttttttct gcgatcgtca taaaggtaat
     2401 agttgaaatt cccctgccac ctggcaaaat atccgttcaa ccatcagctt tgcaggacga
     2461 cctgcaaacg cctcttttca ccggggacgg ccccaaattt ccggagcctg atatgtcctg
     2521 gattatctta gttattgctg gtctgctgga agtggtatgg gccgttggcc tgaaatatac
     2581 ccacggcttt agccgtttga tgccgtgtgg acgggtattg gcgcagtcgg cgcggccatc
     2641 accggcattg tgctgctcgg tgagtccgct aacccgatgc gtctggcgag tctggcgtta
     2701 atcgtattgg ggattattgg tctgaaactc agcactcact aactaccggg ctgctgtacc
     2761 caaataaact tactgacatc aaacccttcc cgggtcgcga ctgccagcat ctcctgtttc
     2821 acttcgtcag aaatggttgg cgtgcgggag agtatccaca ggtagtcgcg gtccgggccg
     2881 caaaccagcg catggcggta ttcccgatcg agtgcaataa cgttataacc gccatagaaa
     2941 ggaccaaaga atgacacttt cagcgcagcg cgagttggtg cgccggtaaa gtacgctttc
     3001 ccttcactct gctgccacat tcctctgtca gggttatagc ctttattaat gacattcagg
     3061 ccgccgtcat cacgcaggct gtatgttgcg gtgacttttt ccagtccacg ttcaaaacgg
     3121 tgatcaaaac gggcaatctc ataccaggta ccgagataac gtttggcgtc gaaattattc
     3181 actacggtca cgccacgcgg cggcgtagga gaactacagg caacgaccag aaatgcagct
     3241 gtcgctgcgg caacgagagg gagcaggcgc ataaatgttt ccttactggt ttttttctaa
     3301 gtgtagatga cagcaaggaa aagcgcagaa aaggtccgaa aattcggacc cgatgaaatt
     3361 ttactgtaga gcgttgagaa tctgacaggc ggcggcgact ctctctggat tgggatagtt
     3421 tttgttagcc agcatcacga tacccagctc tttttctgga ataaacgcga catagctacc
     3481 aaatccgccg gtcgcccccg ttttatgtac ccatgatgcg cgtactgcag gagttggggg
     3541 cttaatcgct tttaccgggc gtgctgccag tgcaatttta ttgtcactgc cgttaatgat
     3601 gctgtcagga ttgaccggcc agtccagcat ttcccagccc aggccctgat acatatcgcc
     3661 ggtttgccag tagcgagatt gtgccagttg tatcccttgt tgaagcgttt tctcattgat
     3721 atcaaggggg ggtaaattgc tttgcaccca gcgggccata tcttcaatgg tcgacttcac
     3781 accataagct tcagcatcta acgcccctgg cgaaacatgc actgccttac cttcgcgata
     3841 tccccaggcg taattctttt cttctggggg aggtacatta atccacgtat ggttgagttt
     3901 gagtggctgg aagacacgag tttgcatcgc ctgctcaaaa ctcaaaccag acggcttcac
     3961 agccagtgcg ccgaacaaac cgatactgga gttggcatac agacgttgtg ttcctggggc
     4021 ccatgcaggc tgccagtttt gatagaagcg cagcaagtcg cttgaggatt tcacttcatc
     4081 cggcacctgc aatggcaggc cgccagcggt gtaggtcgca agatgtaata gtgtgatccc
     4141 attccactgt ttagcggtaa gttcaggcca gtattttgtt gcgggatcgc ttaacttgat
     4201 ttcccctcga gcaatagcgt cgccaccaag cacgcccgta aatgttttgc tgaccgaacc
     4261 taactcaaac aacgtttgct gtgtgacggg ctgctttttg gcgatgtccg catagcccca
     4321 agtaaagtaa taaggtttac cctgataaat taccgccacc gccataccgg ggatcttttg
     4381 ttgctctata agcggggtaa ttgtgcgatg cacaatatcg ttgatttgtt gaggggcagc
     4441 aaatgtggag caagaggcgg taattaataa ggcgcagagc gtcgttttga acataaggtc
     4501 tggtttccat acaaaacggc ccgccatagg cgggccggat ttacattggc gatgcgttag
     4561 attgtaacga caccaatcag cgtgacaact gtcaggatag cagccagacc gtagaaaacc
     4621 catttgcccg caggcacgtg gattttcaga tcgtgcatcg cgtggtgcat acggtgtaaa
     4681 ccacaccaca gcggcagaac gatcatcagg aacaggaata cgcgaccaat gaagctctgc
     4741 gcgaacgcca gaacgcgctc gtagctcagc gcatcacccg gaaacagccc cagtggcagc
     4801 agaataccca ccagcaggat catcaccggc gcaataattg cgctccacat accaccggcc
     4861 ccgaagaggc cccagaatac cggttcgtca gaacgctttg gatttggatt aatcatctca
     4921 ggctccttac cagtacaggg caacaaacag gattacgatg gtggcaacca cagttaccgc
     4981 ccagagactt ttgataattg gctctggtcc cattttttcg tcttttacaa tgatattggc
     5041 cgctttcggt gccagttcaa accaggtttt ggtgtgcagc agagctgccg tcagagtgat
     5101 caggttaatg atcacgataa ccgggttttg taaaaagtcg acgaatcccg cccaggcttc
     5161 cgggccattt ttcagggcaa acagcccgaa aatcagttca atgctgaacc acacagccgg
     5221 aaccgccgtg ccttcgcgca gcatgtaaaa gcgataaaac ggcaattttt tccaccaggt
     5281 ggacgtcatt ggccgtacat acggtttacg tttagtcgtc atgttgcact ccttagcgtg
     5341 gtttcagggt cgcgataaga aagtctttcg aactttctac tttgccctgc tgaatggccg
     5401 cagccggatc gacgtgtttc gggcagactt cggagcaata gcccacgaaa gtacagctcc
     5461 atacaccgtt ctggctgttc aactgcgcca tacgctcctt cttaccgtgg tcgcggctat
     5521 cttcgttata acgatgcgcc agcgtaatgg cagccggacc gatgaactct gggttcaggc
     5581 caaactgcgg gcacgcggcg tagcacagac cacagttgat gcaaccggag aactggtgat
     5641 actttgccat ctgcgccggg gtctggatgt tagtaccctg atccgcggtg cgggagttgc
     5701 cgatgatgta cggtttgatc gcttccagac tttcgatgaa gtgggtcata tcgaccacca
     5761 gatcgcgttc aatcgggaag ttagctaacg cttcaacctt cataccgtcg gtgtaatcac
     5821 gcaggaaggt tttacatgcc agttttggca cgttgttaac catcatgccg caggaaccac
     5881 aaatcgccat acggcaggac cagcggtaac tcaggtccgg tgccaggttg tctttgatgt
     5941 agcccagcgc atccagtaat gaggtagttg cgtcataagg cacttcatag aatgcgctat
     6001 gcggtgcggt atcgacttcc gggttatagc gcaccacctc aattttcagg tttttcatct
     6061 cagccattcg ccttctcctt attattggct gcttccgcct tatcggctgc atccgcttcg
     6121 ccaccgtaaa cgcgtttagc tggcggcagc gtagtaatct tcacgtcgct gtactccagg
     6181 cgagtcgtgc catcagcatc gcggaaggcg agggtgtgtt tgagaaagtt gacgtcgtca
     6241 cgctcggtgc aaccttcgtc cagacgctgg tgtgcgccgc gggactcttt acgtgccatt
     6301 gcggagtgcg ccatacattc agcaacgttc agaccgtggc tcagttcaat ggtgtagagc
     6361 aggtcggtgt tgaacacgct ggaggtgtcg gtgatgcgca cgcgcttgaa gcgttcctgc
     6421 agctctgcca gcttgtcgat ggttttctgc atcagttccg gcgtacggta gataccgcaa
     6481 ccttcttcca ttgccaggcc catttcgtcg cggatcttcg cccagttttc gccgccatcc
     6541 tggttaacca gatctttcag acgttgttca acgccagctg cctgcgcttc aattgccgct
     6601 tcgttgccat taccggcagt tgctgcacgc tctgtcgctt gttcaccggc cagacggccg
     6661 aagaccacca gttccgccag ggagttggag cccagacggt ttgcaccgtg cagaccaaca
     6721 gaggaacatt cacccacggc gaacagacct ttaatgcggg tttcacagtt ctgatcggtt
     6781 tcgataccgc ccatggtgta gtgtgcagtc ggacgtaccg gaatcggttc tttaaccgga
     6841 tcgacgccaa cgtacgcttt cgccagttcg cagatgaacg gcagacgttc atgcagtttt
     6901 ttctcgccga ggtgacgcag gtcgagataa accacatcac cacgcggcgt ggagatggtg
     6961 ttgcctttac gccattcgtg ccagaaggcc tgagaaactt tgtcgcgtgg acccagttcc
     7021 atatatttgt ttttcggctc gcccagcgga gtttccgggc ccatgccgta atcttgcaga
     7081 taacggtagc catttttgtt gaccagaata ccgccttcac cacggcagcc ttcggtcatc
     7141 aggataccgg aacctggcag accggttggg tgatactgaa cgaattccat gtcacgcagc
     7201 ggaacgccgt ggcttagcgc catacccata ccgtcaccgg taacgatgcc gccgttggtg
     7261 ttgtaacgat aaacgcgacc cgcaccgccg gtagccataa cgaccgcgtt agcacggatc
     7321 tgcaccaacg tgccttccat catgttcatt gctaccaggc cgcgaacatg accatcatca
     7381 accagaatat ccagcacgaa atgttcgtca aaacgctgga tctgcgggaa ttgcagagag
     7441 gtctggaaca gcgtgtgcag catatggaag ccggtcttat cggcggcgaa ccaggtgcgc
     7501 tcgattttca tgccgccgaa gcgacgtacg ttgacgctac catccgggcg acggctccat
     7561 gggcatcccc acagttccag ttgggtcatt tcggttgggc agtggtggac gaaataatcc
     7621 acgacatcct gctcacacaa ccagtcgcca cccgctactg tatcgtgaaa gtgatattcg
     7681 aagctgtcat gatcctgcgc gacagcggcg gagccccctt ctgcagcaac ggtatggcta
     7741 cgcatcgggt atacttttga gattagtgcg atttttgcat ttggatttgc ctgcgcggca
     7801 gcaattgcag cacgtaatcc cgcgccaccg gcgcctacaa tggcaagatc ggcttgaaag
     7861 gtttgcacga cattcctcca gattgttttt atcccacagc cccgtacttc agggtaagtg
     7921 cctgaaagtt acggtctgcg aacgctattc cactgctcct ttataggtac aacagtatag
     7981 tctgatggat aagtctgaaa tttgacgaga tcgatttttt tagtgcgcaa gagggctaaa
     8041 ttatcactga agatgattaa tttaattact aaaccatcag atcgtgcttt ttttagtcac
     8101 tgcccaccgc tgtttgattc ctgcgtaaaa tttgtctcgc cgtcgcgttg cgagtagact
     8161 tcgtgccctt gtcaaaaact ggagatttaa ctatgagcga aacggcatcc tggcagccga
     8221 gcgcatccat tcctaactta ttaaaacgcg cggcgattat ggcggagatc cgtcgtttct
     8281 ttgccgatcg tggagtgctg gaggtggaga cgccttgtat gagccaggcg acggtaaccg
     8341 atattcattt ggtcccgttt gagacacgtt tcgttggccc cgggcattcg caggggatga
     8401 atctctggtt aatgaccagc ccggaatacc atatgaaacg cctgctggtt gctggttgtg
     8461 ggccggtatt ccagctgtgc cgcagcttcc gtaatgaaga gatggggcgt tatcacaacc
     8521 ctgagttcac tatgctggag tggtatcgac cgcactatga tatgtaccgg ttgatgaacg
     8581 aggtggacga tctcttacaa caggtgctgg actgcccggc agcagaaagc ctttcttatc
     8641 aacaagcttt cttgcgttat ctggaaattg acccgctctc tgccgacaaa acgcaactgc
     8701 gggaagtcgc agcgaaactg gatttgagca atgttgctga taccgaagaa gaccgcgaca
     8761 cgttgctaca attgctgttt acctttggcg tagagccaaa tattggtaaa gaaaaaccga
     8821 cctttgtgta ccactttcca gccagccagg catcactggc gcaaatcagt accgaagatc
     8881 atcgggtcgc tgaacgcttt gaggtttatt ataaaggtat tgagctggcg aatggtttcc
     8941 atgaattaac ggatgcccgt gagcagcaac aacgctttga acaagataac cgtaagcgcg
     9001 cggcgcgcgg tttgccgcag caccccattg accagaatct gattgaagcc ttgaaagtcg
     9061 gtatgcctga ctgctccggc gtggcattag gtgttgatcg tctggtgatg ttggcgctgg
     9121 gcgcggagac actggctgaa gtcatcgcct ttagcgttga ccgggcataa ttctgaaaaa
     9181 ttactgaatt aacagcgaag aatggcgtga taagcgccat tctgtagcat atgttttcat
     9241 ttttccgtta aaggccacaa tttaagactg gatatttgat atcatcaagg tatcaattct
     9301 gtattggttt ttacgctgcc tgctcaatgt tgcgcagtgc agttcagtgg gcacgtgtta
     9361 tacacgcgct gaaatgaagg atggtttcat gcctcacacg ataaaaaaga tgagtctgat
     9421 aggactcata ttgatgatct ttacttccgt atttggattt gccaatagcc catcggctta
     9481 ttacttaatg ggttatagtg cgattccctt ttatatattt tctgcattgt tattctttat
     9541 tccattcgcc ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat
     9601 ctattcctgg atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg
     9661 gttttcctct tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc
     9721 aacattcctc tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac
     9781 gcaggtggtt ggtctgctgg cagtagcatg gatgattctg gtcaccgtcg ttgcttcaaa
     9841 ggggattaat aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa
     9901 tttagtattg ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca
     9961 ggatattaat ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc
    10021 gtttgtggta tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga
    10081 taaaacggaa aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat
    10141 ttcaatcggt tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt
    10201 attaagtaat ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg
    10261 ggtgacgctg ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg
    10321 gtttgcgcgt attaccggac tttcgatgtt cctcgcttat accggtgcgt tctttacgct
    10381 ttgctattca ccgctgaaag ccatcatcca ggggacgccg aaagcgttgt ggccggaacc
    10441 gatgacgcgc ctgaatgcga tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt
    10501 ggttactgtc ttcattctgc tggtttcgtt tggtggcggt accgcatcgg cgttctttaa
    10561 caagctgacg ctgatggcga acgtgtctat gacgcttcct tacctgttcc tcgcgctggc
    10621 tttcccgttt tttaaagcac gtcaggatct cgacagacca tttgtgattt tcaaaacgcg
    10681 tatgtcggca atgattgcga cggtggttgt cgttctggtg gtgacatttg cgaacgtctt
    10741 caccattatt cagcctgtgg ttgaagccgg agactgggac agcacattgt ggatgattgg
    10801 cggccctgtc ttcttctcgc tgttagcgat ggcgatttac cagaactatt gcagtcgcat
    10861 ggcgaataaa cctgagttag ctctcgactg atcatatgaa atcctgcaac tcatcgtctc
    10921 tccggcgatg agttgtcagg aagtaatgcg tatggatgat actctccagg gccggtgata
    10981 actgaagatg atgttaagct gctaaatgag ccacgttgac atcatttggc agcttcatgc
    11041 attacaaact tcccgcctga cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt
    11101 taccgttgag gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg
    11161 cgtggaagcc tgccagaatc agctggtgga tctcatggcg tagcggcata cggtgaccca
    11221 tctcagcggc gtaaatacgc agctcgaaaa tctgaatccc ctgttgcaga tccaccagga
    11281 agacttccgg tgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga
    11341 tttccgtcac ttcttcactg ttggcatcgg caggggccgg aatcgtcaac actacgcgcg
    11401 tgaccgagtc agagagcgac cagttgataa actgctcggt aataaacgcc ttgttcggca
    11461 cgattatctc tttacggtcc cagtcgctga tggtggtggc gcgggtgtta atttttgtca
    11521 cgctaccagt aagatcgcgg atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca
    11581 ggatgatcag gccagagata aagttggcga aaatttcctg caaaccaaaa ccgagaccaa
    11641 caccgagcgc ggcaaccagc cactgcaatt tcgaccactc aataccaatc attgagaagc
    11701 cgaccagccc gccaatcagc atcagcagat attttgtgat ggtggtgatg gcgtaacccg
    11761 tacccggcgt taaatccagg tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc
    11821 gcaccagctg cgtggtgatg ataaatacca gaatggcaat cagcaccgca ccgagggtaa
    11881 ttggctccag actttctacg ccctgtaccg tggaggtgac atcccacagc gaaatatttt
    11941 cgaggaagcc gaaagcggaa tggatttctg accacagcac aatgaccgaa agcagggcga
    12001 tcaacatcaa aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga
    12061 cttcgctttc atcgacttca attgctcctt ccgggctact gtgatgatgc gcttcctctt
    12121 cgccacgcgc acgttgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca
    12181 gcctgcgacg ctggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga
    12241 accagatggc aatcgaggtt tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac
    12301 ccaccgccga cgccagaatg gcaaccagtg gtgcgccaat catcatgttc cacagcatat
    12361 ggttggtaat gttgtcgccg ctgccttctt tgttgagata caacggaata ccggcttttt
    12421 tcaggcttag ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccgtccca
    12481 gcgaaccgga aaattcgcgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc
    12541 cgatgctcat caggtagtag cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat
    12601 gagcgataaa caagccgttc gggcgggcaa aggtggcgca aatcatcact acccacagca
    12661 gcggcacggt ggccgttaca ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct
    12721 cgcgcaagcc gtaacccagc gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg
    12781 accagaaaag agtgcgcaac gtcagccaga agtgatcctg agtcactttg ccgactttcg
    12841 ccgccgaacg ttcaagaaaa cgggtgaaat agcggcgcga gtaaatactg caaccgacca
    12901 gaatcaacgc gccaaacagc ggcaaaattg tctctttgct ggtcagcatc atcacactgg
    12961 ctttgcccaa ctgactgaag gtgtccagcg aaatgagacg acgcagatcc tgggcgattt
    13021 ccagcggcca ggcgatggtc atcgggcgca cgtcagaggt ccagaacaga tagcggtgcg
    13081 ttgcttcgtt cacctctttc agcgcatcct ccagttgccc gttggagact ttcagcttgg
    13141 tcagttccaa tagtagcgtg tcgccaccct gcaacaatga gttcagcaac tcacgctgag
    13201 tgcgcagttg tgcttccaga atacggtttt gctcggcggt cagcggctga ccgtcggcct
    13261 gatggatttg ccgtagcagc ggctgtttat taagtaaatc ctcataacgt aaccgttgca
    13321 cacgcaactg cgccatttcg gtatcaagct gttgtggttt tggcatttct ggtagccgtg
    13381 ccacctgcgc ccgcagcgct tcgccgagca ggttggacga tcccagccat tgcgactgtt
    13441 cacgcagcgt attcaacgcc tgccggacct gtaacgtctg gctggcagcc tgacgctgtt
    13501 gcgaggcaac gagctccatc cgctgcgcct gttgattcag cgctgccgat agttcgcggt
    13561 taattttgaa ttgcgcgacg atatctttcg gcaaatcggc actgttttct gccagtaatt
    13621 cggtactttc cagcgcccgc tccgcctcaa gctgacgttg gctattcaac tgattacgta
    13681 agccctgcaa atacgcatcc agttgctggc tctctttttc cgccagctct gagcgtaagc
    13741 gcgctaattc ctggcggtta ttggcagaca gctgcgccag ctccagttca tcaacgagcg
    13801 ccttaagacg tgcagagtca gactgcaacg cgaaattttg tgcctgattg agcggagtat
    13861 tgccggtaag cgttcccagg cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt
    13921 gctgttgcgg cagttgattc agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct
    13981 gggcctgacg gcttttatcc agcaactggc tgctgacctg gagaatttcc tgattcagcg
    14041 cgtcggtaga cattcccggc gacacgctgc gcggctcgtc acgcatgttg tttaattgtg
    14101 cgcgcagagt agcggagagt ttcggataat tatcgataac ttgctgatat tgtttgatgc
    14161 gctcaaggga accttttcgt tcctcaagcg catttaaggc agactggagc gcctctacga
    14221 cttccggctg tgcgggtttc gccgcttttg cctgctccag ttcctgagtg atttgtttgc
    14281 tatcgggggc cgtcgcggcg tacgcccccc aactgaggca ccaggccatc agaaaagtga
    14341 taatcaggcg cacgtcagcg tttcctttga tggattagac ctggtctttt ttatcgtcaa
    14401 ccaatggcct ggcgtcgtgt tctgcttcga tctcttcagc aggaagtggg gcgggttcag
    14461 cgtctggcgt aacaaaggtt tcggtagata ctgccagcgg ctggccaatt ttcgtgacag
    14521 acaggctttc cagttgctca accagattca ctttacccgg tgcaaacagg ttgataacgg
    14581 tggaaccgag tttaaagcga cccatttcct ggcctttcag cagtgccaca gaaccgtcgt
    14641 tttccccggc aggccaggtc cagcgcttga tgataccttc gcgcggcggc gtaatggtgc
    14701 ccgcccagac cgtctcaatg ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg
    14761 ggccaaattc ggtatcgaaa aggcaaatca cgcgttcgtt acgggcaaac agattcggca
    14821 cgttctgagc ggtgagatgg ttaacggaaa agagatcgcc cggcacgtag atcatctcac
    14881 gcagaatacc gttgcacggc atgtgtacgc gatggtagtc acgcggggag aggtaagtgg
    14941 tcacaaacgt accgttgcgg aacaggtccg ccatcagata gttgcctgcc agcagggctt
    15001 cgaggctgta gttgtggcct ttggcttgca ggattttatc ttcttcgatt ttacccaact
    15061 ggctgataac gccatcggca ggcatgacca gtacattcgg atcggtatcg attgggcgta
    15121 cttcgtcacg cagcggacgg acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg
    15181 gcttttgcgc ctctttcatg tcgaccttgt agtatttaac gaacagatcg ataaccagtt
    15241 ttgtcagcca tcctgcccgc ttgcttgcgc cccaacccgc caggcgagta agccatagtt
    15301 tcggcagaat gtactgtagc gaaagtttaa atgaatttaa caaggtagcc tccaggccat
    15361 tgttttgtcg ttcctgatcc ggccaacatg ccggatcctg aaaaaaaggg gacgattcta
    15421 acgacggtta gcttaattgt cagccatccg tatcagaaaa gtttttacgc gtttttacct
    15481 gcgccatgct ttccagaata cggtgatagt tttcgaaacg ggtttccgcg attttccctt
    15541 cctcaaccgc ttcccggata gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt
    15601 tacacagacc taaatagtca tggaattcga caaagccctg agtgatttgt tccggctcca
    15661 ggtgccagag gccgaactca cgcacaccag gggaatcaat cacatcaccg ccgtgcggga
    15721 agtgatacag ccgagcggcg gtcgtggtgt gctgaccgag ccccgagttg tcagagacat
    15781 cgttggtcag gatctctttt tgcagcccca gcaacgcgtt cagcaggctg gatttaccga
    15841 cgccagactg cccggcaaaa atgctgatgc gcccggtcaa cgcctcttcc agcggtttca
    15901 gcccatcctg agtatggctg gaaaccatca atacgcgata accgatgttg cggtagatat
    15961 ccatctgctc gttgacgaac gccatgcctt cgtcgtccag caggtctatc ttgttgagca
    16021 cgataatcgg ctcaatctgc aaggtttcgc aggccaccag gtaacggtcg ataatattga
    16081 gcgacagctc cggcaaaatg gcggagacaa tgacaatctg gtcgatgttg gcggctatgg
    16141 gtttcacgcc gtcgtagaaa tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt
    16201 ccacgatccc tttgatattt acgccttccg ccgccggttt acccggacgc cagactacgc
    16261 ggtcgccggt taccagcgaa cggatggtac ggcgaatatt gcagcggtga acgtcgccat
    16321 cggcggattc cacatcagcg tgcataccaa agcggctgat gacgatacct tcatcaggct
    16381 cgccaaacag attgtcgtcg tagtcgggct tctccttaga cgttttaaga cgacgctggt
    16441 gattggcgtt cacgcggcgc tgctggcctt tggagagttt atttttactc aatcgtactg
    16501 actcctggtc gcccctgatg ggcaaaacat ctatgataca cgcaattgtg gatcaatata
    16561 gtcactgtga atgggtggaa aatagcatga gtgccaatga aaacaacctg atttggatcg
    16621 atcttgagat gaccggtctg gatcccgagc gcgatcgcat tattgagatt gccacgctgg
    16681 tgaccgatgc caatctgaat attctggcag aagggccgac cattgcagta catcagtccg
    16741 atgaacagct ggcgctgatg gatgactgga acgtgcgcac ccataccgcc agcgggctgg
    16801 tagagcgcgt gaaagcgagc acgatgggcg atcgggaagc tgaactggca acgctcgaat
    16861 tcttaaaaca gtgggtgcct gcgggaaaat cgccgatttg cggtaacagc atcggacagg
    16921 accgtcgttt cctgtttaaa tacatgccgg agctggaagt ctacttccac taccgttatc
    16981 tcgatgtcag caccctgaaa gagctggcgc gccgctggaa gccggaaatt ctggatggtt
    17041 ttaccaagca ggggacgcat caggcgatgg atgatatccg tgaatcggtg gcggagctgg
    17101 cttactaccg cgagcatttt atcaagctgt aaaatttttc ctggtcacgt aagcgccgga
    17161 atccacttgc ggcggcgtga ttttgacgct aaatcgaacg tcttgctgat aatttcagca
    17221 cttgagataa aaacgcaaaa aaaacttttt tggggggttg cagagggaaa gatttctcgt
    17281 ataatgcgcc tcccgtaacg acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt
    17341 atgcgggaat agctcagttg gtagagcacg accttgccaa ggtcggggtc gcgagttcga
    17401 gtctcgtttc ccgctccaaa atttgaaaag tgctgcaaag cacagaccac ccaatcgcag
    17461 tatttcgaat tgcgacaagg cggcaactga acgaatcctt gggaacttac aaaagtatgt
    17521 gactaaggtg agtgaaggca gccaaggcag tagcggttcg aaagacgaag at
//
LOCUS       DAJTYF010000076        17397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTYF010000076 DAJTYF010000000
VERSION     DAJTYF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.076"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="OO590_002686"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="OO590_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6637995.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3172)
                     /gene="hemX"
                     /locus_tag="OO590_002687"
     CDS             complement(1967..3172)
                     /gene="hemX"
                     /locus_tag="OO590_002687"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6637996.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3194..3934)
                     /gene="hemD"
                     /locus_tag="OO590_002688"
     CDS             complement(3194..3934)
                     /gene="hemD"
                     /locus_tag="OO590_002688"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6637997.1"
                     /translation="MSILVTRPSPAGEELVRRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3931..4872)
                     /gene="hemC"
                     /locus_tag="OO590_002689"
     CDS             complement(3931..4872)
                     /gene="hemC"
                     /locus_tag="OO590_002689"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6637998.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5259..7805
                     /gene="cyaA"
                     /locus_tag="OO590_002690"
     CDS             5259..7805
                     /gene="cyaA"
                     /locus_tag="OO590_002690"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6637999.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7845..8165)
                     /gene="cyaY"
                     /locus_tag="OO590_002691"
     CDS             complement(7845..8165)
                     /gene="cyaY"
                     /locus_tag="OO590_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6638000.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8213..9526)
                     /locus_tag="OO590_002692"
     CDS             complement(8213..9526)
                     /locus_tag="OO590_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6638001.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9523..11001)
                     /locus_tag="OO590_002693"
                     /pseudo
     CDS             complement(9523..11001)
                     /locus_tag="OO590_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            11190..11393
                     /locus_tag="OO590_002694"
     CDS             11190..11393
                     /locus_tag="OO590_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6638002.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11430..12254
                     /gene="dapF"
                     /locus_tag="OO590_002695"
     CDS             11430..12254
                     /gene="dapF"
                     /locus_tag="OO590_002695"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6638003.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12251..12958
                     /locus_tag="OO590_002696"
     CDS             12251..12958
                     /locus_tag="OO590_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6638004.1"
                     /translation="MKQLGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12955..13851
                     /gene="xerC"
                     /locus_tag="OO590_002697"
     CDS             12955..13851
                     /gene="xerC"
                     /locus_tag="OO590_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6638005.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13851..14567
                     /gene="yigB"
                     /locus_tag="OO590_002698"
     CDS             13851..14567
                     /gene="yigB"
                     /locus_tag="OO590_002698"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6638006.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14651..16813
                     /gene="uvrD"
                     /locus_tag="OO590_002699"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14651..16813
                     /gene="uvrD"
                     /locus_tag="OO590_002699"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6638007.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16960..17352)
                     /locus_tag="OO590_002700"
     CDS             complement(16960..17352)
                     /locus_tag="OO590_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6638008.1"
                     /translation="MRLGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGAMLMENGVI
                     NPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGT
                     ISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         4058 a         4481 c         4604 g         4254 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatctacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
     2041 caggtttagc ttccgttgtc cccgctgccg gttgtgccag caggttacgc acgcgagtct
     2101 gcatcagttt ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat
     2161 tttgctggct taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat
     2221 cgtagtaagc acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct
     2281 gatgacgcgg tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat
     2341 agatatcctg atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa
     2401 tgaagttgtc cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg
     2461 aactggaaag ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca ttatcggcca
     2521 gacgcaggtt atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat
     2581 ccacctgcga tactgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa
     2641 tcagactcgg gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag
     2701 aggtcgtgac gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg
     2761 cctgagccag cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc ttttgttgga
     2821 cttcatccaa ctgttttgcc agcgtttctt gctgacgatt cgcctgctta agctgcgcag
     2881 cttgttgctt aataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcaatg
     2941 ccgtcagttg gttagccagg gcatcgctgg tggcggtctg attgacggcc tgttgtttac
     3001 cccagccata caaaccgaca cccgccgcca gagcaatagc gatagccacc gcgctgagaa
     3061 tcaatgcagt attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca
     3121 cggcctccct ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt
     3181 cctgttatga gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga
     3241 ccttaatgtc ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca
     3301 atagtcgaca gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt
     3361 gctgcaacat ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat tgccagcgca
     3421 tcgcttcttc tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga
     3481 cctcagcacc gcgcaccgtc agggtatccc caattagctc acgaccgcca ttgccacgta
     3541 atatcagcgc acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc
     3601 tgatttcccg atcctgtggg tagagaatct tctgtccgct tacggtatgt agcgccagtg
     3661 cggtggtgcg tccaatggtg aaataagtag gtagttgggg ccatttacga tcttgctgat
     3721 gcagctgtga ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct
     3781 cccccagcgc tgccagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa
     3841 tcagcggaaa atgccaggcc acctgcccca gtgtgcgcag acgtctcact aactcttctc
     3901 cagcgggaga cgggcgggtg accaggatac tcatgccggg gcctctccgt tatagacttc
     3961 agcgaggatc tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg
     4021 ttcggcatct tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc
     4081 gccgaccagc gcacatagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg
     4141 cacctgacat ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc
     4201 agtttcgtgg tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca
     4261 ttcaataccg actgcacctt gtcctaccgc cggaagagaa atctcgggtg gtaacgcggc
     4321 gcgaatgcgg gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc
     4381 gtattcgccg ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat
     4441 aatcagatcc gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac
     4501 gatactgcct gctggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg
     4561 aggatcttca cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac
     4621 atctttcatt gaatgtacgg cgatatcagc gcgattctcc aggagcgcga cttccagctc
     4681 ttttacaaat aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc
     4741 gcgcgtcacc atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc
     4801 tttgacatag tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac
     4861 attgtctaac atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca
     4921 gagtgatgtc agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg
     4981 gcagtggatc ctgacaggcg tttcacgccg tcgtaataag gaatttacag agaataaacg
     5041 gtgctacact tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga
     5101 tcacgtttta gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa
     5161 cggttacctt tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga
     5221 atcacagtca tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc
     5281 tgaaacagag actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg
     5341 ggcctgcatt ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc
     5401 taatgccggg ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg
     5461 aaactcaacg ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc
     5521 caccgaaagg tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag
     5581 ggcaaagttg ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg
     5641 aagagcgcca gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg
     5701 gtgtggaagt cagcttcttc ctgattgatg aaaaccgctt ccgtcataat gaaagcggca
     5761 gcctgggggg cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc
     5821 gtactgccgt gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag
     5881 aagagcatta cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg
     5941 aatggctgga tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc
     6001 tttggcagct ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc
     6061 tggaagccta ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc
     6121 gtttgcacga cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc
     6181 gtgttactga atacctgacg gcgattgaag atttcacccg tctggattta gtacgtcgct
     6241 gcttctattt aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta ggctggcgtc
     6301 gcgcagtgtt gagccagtta gtgagcgagt ggggttggga cgaagctcgt ctggcaatgc
     6361 tcgataaccg tgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg
     6421 acgcgatgat gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg
     6481 tctccgccag tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc gcgtttgaag
     6541 cattgccagg taaagtgacg ctggtaaacc cgcagatttc acccgatctc tcggaaccga
     6601 atctgacctt tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata
     6661 accgcgcgcc aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc
     6721 tgaataaact ggtggcgtgg gcatggttta acggcctgct gacctcgcgc acccgtttgt
     6781 atattaaagg taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt
     6841 cgcaccattt cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg
     6901 agattcgcca tctggcgatt atcgttaacc tggaatatga cccgacagct gcgttccgca
     6961 atcaggtggt gcattttgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa
     7021 attgcctggt aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc
     7081 tgcacttcaa cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc
     7141 atcaggacgc cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg
     7201 gcttaatccg tactcgcgtg cagcaactgg tttctgagtg tattgaactg cgcctttcca
     7261 gcacccgcca ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct
     7321 tgttcttcga acgcctgaat gtatcggtac agaagctgga gaacgccatc gagttttatg
     7381 gcgctatttc gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc aatcacgtca
     7441 aattaccggc ggtggtggac ggctttgcca gcgaagggat catccagttc tttttcgaag
     7501 aaacgcaaga cgagaatggc tttaatatct acattctcga cgaaagcaac cgggttgagg
     7561 tgtatcacca ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt cgcttctact
     7621 cgtcatcgca cgaccgcttc acctacggct caagcttcat caacttcaac ctgccgcagt
     7681 tctatcagat tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga acaaaatcga
     7741 ttggcaacct gccgcccgcc aatcaggatc acgatacgcc gctattacag cagtattttt
     7801 cgtgatgagc gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact
     7861 gtttcacccg cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg
     7921 cgatcgcaaa tccattcatc gcctttcagg tcaaaatggt agccgccctg tttggttgcc
     7981 agccacacct ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc gttttcaaag
     8041 gtaatggtca gtacgccgcc gttgatttcg cagtcgatat cgctgtcgcc atccctgtcg
     8101 tccagacgct cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg
     8161 ttcattgttg taccctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct
     8221 tcaaagtttg cacctctgct gctgcgttcc ggcatgattc atccgtcacc ggaataatga
     8281 tgtctctgtt ttgaacttca cgtacgttct cctctctgac acgagttttc gccgggcagg
     8341 gggagggcgt aatccttacc tctgtatcgg tccatgtata aacattgcga tcgtcataag
     8401 cgaagcgatc gtttttagtt agcggaccgt taactggaat aatttgcaag ctctttgacc
     8461 gtaattggcg gataaagtaa gcatgctgtt tgccgctatt aaatgagcga tcaatgacca
     8521 agtctggcgt atccagcggg attatctcaa gctggtcttc accaaatgtc gataattttg
     8581 ttacatacag ccgatcttta tattgcgcaa tattgccagt aatcagatgg aattgttcag
     8641 gttcaatacc tggtaggtct atccacttgc tccactgacg gtcgggcgtg agctgtttac
     8701 gccagcgaac gcgactttcc cctacttcaa aagagacgtc atcatctacg ggtatcgctt
     8761 tgcctgataa ttgaccaaag ggataacgat gcagaccctt attgtcgcga aaaaccagca
     8821 gtgaatgagg tatccagcga attatctcaa atgagtctgc attcgccgta aggtgaacgc
     8881 catttaaata aatattctgc ccactgcgga taagcagatc attggaatat gaatttctgt
     8941 ttacactcat aagacgtgga ttaatacccc accatctttt ttctaataca gtaacatcac
     9001 tgctgctccc ctgacgtcga ccggacaagt agagattttt actatccacc agcgtggtag
     9061 agttaccttc gatagctttt agtgtcgcca gatcaacacg tgacgcgccg ctattacttt
     9121 cggtgcgctg tccgtcaaaa tagaggctat atttatcgac cgcgaagcgg cccatagcct
     9181 gaaagctggc aatatcgaca gtgggtgttc ccggtggatt gctaacgatt ttgccacgcc
     9241 agagaataac gcggccgtct gtcacccagt ggtattcctc attttctatg cttatttcat
     9301 cggaaggtat ataacccgtt ttcccagaga tatttttcac aaggcgcaaa ccggccctgc
     9361 tcatctccgg aaactgggca atgattttct tatcccccca ataaccttta ccgtaagaca
     9421 taacaccgtg atcattgtaa tagtcgaacc ctatggcctg tacagcaggc gtcagaaatg
     9481 tgtgtcccaa caggatgagt gcaactgtga ttatttgacc cttcattttg tcacctcatt
     9541 gcgcggggca ggaataaatt cgccgtcatc agtaattttt ccgtccagat atcccgtttt
     9601 gtaatttacg ccatagacat atcccgaatg ttgttccttc cagattaagc gaacttgttt
     9661 cggatcagcg gtgcggtatc gccagagctg cgcgccatca aagctgtaaa tgtagcggtt
     9721 atcgcgggca taccatctgc gcaaaaaggg atggtcatta agatgggctt cgcgttcatc
     9781 cggagaacgg ttatccagta agatcagcgg tccgtcagat tcaaacacgt gtaaaccgga
     9841 atggtatggc tacccttcgg cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa
     9901 acgtttgttg ataataagat tgggatcatc aagtttcacg atctccagct tacggtcacc
     9961 agactctgtt tcgatgatgg tataaagcga gtcctgatac tgggcgacag catcactgat
    10021 gggttgaaac tgctccggat cgagtccggg taattcttcc tgtttacaag caggtccttt
    10081 acgccagaac actttctttt ccagcagaga aaaatccaga cagtgctttg ttaaatcttc
    10141 atcccacgcc agattttcct tgtcgaggac tttacgctgc agcccgtttt tatcacgcca
    10201 ggtcaagagt gagccaggca tccagcgcac gacggaaaag gtgtccgggt cggcatcaag
    10261 ctgttcgccg ttgagcagga ttttggtccg tgtacgagcc agggtatccc aggggccaaa
    10321 tggtacggca atgcaggggt taaacgtggt ggaaaacttt cctcgctgat cccaggactt
    10381 ctgcgccagc acacggaaac tctcggggtc agcgaggcga tgcccattga tatacaagaa
    10441 attagcgtca cgtaatatca gccccagtaa gtcaggatcc cagggaggcc ggaattccac
    10501 ctgatgcagc gttttgatat ccaagctatt tccaccacca ttgtcatcgg tgcgcttgcc
    10561 ttcaaaatag agactgtgtt tatctgcggc aaaatcaccc catgccttga acgaggcgac
    10621 atcgacggga ggtgtgccgg gagggttttg cacaacttca cccgcgtaga ggatattgcg
    10681 accatcgcta tgccatgaat aatgaggata ctaggcctgt gcgccctcat agaggtagtc
    10741 ggttatcaaa tcagtcagag gcggttcttt atcccattcg tctagtgttg tcgggtctat
    10801 taataaattg ggtaaccgac gcaaactctt gtaattcacg tcaggtagag aaagatttgt
    10861 ttctttataa gggcgcaggt acaccacgcc ttttccctgt ttctggtaaa tattatgttc
    10921 aggtgcggaa agccgacatc cccatgccga cgataaaaac aatgaacaag ccaacaaaca
    10981 aaccacattg cgatagtgca taaagccatc ctggcgcgag gtgttgatca cgaaactgcc
    11041 agcagaacat aaatcctcac gagtaagcgt tatactcgca gcatttcctc acttttcaga
    11101 cttcataaag agtcgctaaa cgcttgcttt tacgccttct cctgcgatga tagaaagcag
    11161 aaagcgatga actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat
    11221 tacttactct cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg
    11281 cagataaaaa cgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc
    11341 cggataaaaa cgatcgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag
    11401 tttctgtacc cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca
    11461 acgattttat ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc
    11521 gtcgcctggc ggatcggcac ctgggggtag ggtttgacca gctgctggtg gttgagccgc
    11581 cgtatgatcc tgaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtgg
    11641 cgcagtgcgg taacggtgcg cgctgctttg cccgttttgt gcgtctgaaa ggactgacca
    11701 ataagcgtga tatccgcgtc agcaccgcca acgggcggat ggttctgacc gtcaccgatg
    11761 atgatctggt ccgcgtaaat atgggcgaac ccaacttcga accttccgcc gtgccgtttc
    11821 gcgctaacaa agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg
    11881 gcgtggtgtc gatgggaaat ccgcattgcg tgattcaggt cgatgatgtc gataccgcgg
    11941 cggtagaaac gcttggtcct gttctggaaa gtcacgagcg ttttccggag cgcgccaata
    12001 tcggttttat gcaagtggtt aagcgcgagc atattcgttt acgcgtttat gagcgtgggg
    12061 caggagaaac ccaggcctgc ggcagcggcg cgtgtgcggc ggtggcggta gggattcagc
    12121 aaggtttgct ggacgaagaa gtacgcgtgg aactccccgg cggtcgtctt gatatcgcct
    12181 ggaaaggtcc gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat
    12241 ttattcatct atgaagcaac taggggaaga actgcaggaa acactcacgg agcttgatga
    12301 ccgggcggtt gtcgattatc tgattaaaaa tcctgagttt tttatccgca atgcgcgcgc
    12361 agtagaagcg atacgtgtgc cgcatccggt acgcggcacc gtttcgttgg tcgagtggca
    12421 catggcccgc gcacgtaatc acattcatgt tctggaagag aacatggcgt tgttgatgga
    12481 gcaggctatc gccaacgaag ggctgtttta tcgcctactc tacctgcagc gcagtctcac
    12541 cgccgccagc agtctcgacg atatgctgat gcgctttcac cgctgggcgc gcgatctcgg
    12601 cctggctggt gcgagtctgc gcctgtttcc agatcgctgg cgcttaggtg cgccgtcgaa
    12661 ccacactcat ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtttggg
    12721 gcaggaacag cactatcttg ggccgcttaa cggaccggag ctgctggtgg tgctaccgga
    12781 agcgaaagcg gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttgggtgt
    12841 tgtgctgttt accagtcgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact
    12901 tcatgaaatt gcgctgatgt tgccggagct tctggagcgt tggattgaac gcgtatgacc
    12961 gatttacaca ccgatgtaga acgctaccta cgttatctga gcgtggagcg tcagcttagc
    13021 ccgataaccc tacttaacta ccagcgtcag cttgaggcga tcatcaattt tgccagcgaa
    13081 aacggcctgc aaagctggca acaatgcgat gcagcgatgg tacgcaattt tgctgtacgc
    13141 agtcgccgta aagggctggg agcagcaagt ctggcgttac ggctttctgc gctacgtagc
    13201 ttttttgact ggctggtcag ccagaacgaa ctcaaagcta acccggcgaa aggagtttcg
    13261 gcaccgaaag cgccgcgtca tctgccgaaa aatatcgacg tcgacgatat gaatcggctg
    13321 ctggatattg atatcaacga tcccctcgct gtacgcgacc gtgcaatgct ggaagtgatg
    13381 tacggcgcgg gtctgcgtct ttccgagctg gtggggctgg atatcaaaca cctcgacctg
    13441 gagtccggcg aagtgtgggt gatggggaaa ggcagcaaag agcgccgcct gccgattggt
    13501 cgcaacgctg tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa
    13561 gacgacgcgc tttttctgtc gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa
    13621 cgctttgccg aatggggcat aaaacaaggg ctgaataatc acgttcaccc acataaatta
    13681 cgtcactcgt tcgctacgca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag
    13741 ctactgggtc atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac
    13801 cttgcctcgg tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt
    13861 accggccttt ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata
    13921 accgtccggt gattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc
    13981 cggcgctgcg cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag
    14041 cggaacccga gatttatcac gacgtgacgc gttggcgttt tcgttcgatt gaacaagcga
    14101 tgctcgacgc cgggctgagt gccgaagaag ccagtgcagg cgcacacgca gcaatgatta
    14161 actttgccaa atggcgcagc cgaatcgacg tcccgcagca aactcacgac accttaaaac
    14221 agctggcgaa gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt
    14281 ttggtttggg ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac
    14341 cgttcagcga tatgtacttt ttggctgcgg aaaaactcaa cgtgccgatc ggcgagatct
    14401 tacatgttgg ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg
    14461 cttgttggat cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc
    14521 cacatctgga aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt
    14581 atatataccc agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc
    14641 ggcggtgcca atggacgttt cttacctgct cgacagcctt aatgacaaac aacgcgaagc
    14701 ggtggccgcg ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg
    14761 cgtactggtg catcgtatcg cctggctgat gagcgtggaa aactgctcgc catactcgat
    14821 tatggctgtg acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcggacaact
    14881 gatgggcacc agccagggcg gcatgtgggt aggcaccttc cacgggctgg cgcaccgcct
    14941 gctgcgtgcg caccatatgg acgccaatct gccgcaggat ttccagatcc tcgatagcga
    15001 agaccagcta cgcctgctta agcgtctgat caaagccatg aacctcgacg agaagcagtg
    15061 gccgccgcgc caggcaatgt ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca
    15121 tcatattcaa agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta
    15181 tcaggaagcg tgcgatcgcg cgggcctggt ggacttcgcc gagctgctgc tgcgcgctca
    15241 cgaactgtgg cttaacaagc cgcatatcct acaacactac cgcgaacgtt ttaccaatat
    15301 cctggtggac gaattccagg ataccaacaa cattcagtac gcgtggatcc gcctgctggc
    15361 gggcgacacc ggcaaagtga tgatcgtcgg tgatgacgac cagtcaatct acggctggcg
    15421 cggggcgcag gtggagaata ttcagcgttt ccttaatgat ttccccggtg ccgaaattat
    15481 tcgtctggag caaaactacc gctctaccag caatattctg agcgccgcta acgccctgat
    15541 tgaaaacaat aacgggcgtc tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc
    15601 tatttccctc tattgcgctt ttaacgaact cgatgaagcg cgttttgtgg ttaaccgcat
    15661 caaaacctgg caggacaacg gcggggcgct tgccgagtgc gccattctct accgcagtaa
    15721 cgcccagtcg cgtgtgctgg aagaagcact gttgcaggcg agtatgccgt accgtattta
    15781 cggcgggatg cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct
    15841 gattgccaac cgtaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg
    15901 tattggtgac cggacgctgg acgtggtacg tcagacatcg cgcgatcgcc agttaacact
    15961 ctggcaggca tgtagtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc
    16021 cttacagcgg tttatggaac tgatcgacgc cttagcgcag gaaactgccg atatgccgct
    16081 gcatgtacag actgaccggg taattaaaga ctccggcctg cgtaccatgt acgagcagga
    16141 gaagggcgaa aaaggtcaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg
    16201 ccagttcagc tacaacgaag aagacgaaga tttaatgccg ctgcaggcgt tcctatccca
    16261 tgcggcgctg gaagcaggcg aagggcaggc ggatacctgg caggatgcgg tacaactgat
    16321 gaccatgcac tcggcgaaag gtctggagtt cccgcaggtg tttatcgtcg gtatggaaga
    16381 gggcatgttc ccaagccaga tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg
    16441 tctggcctac gttggcgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac
    16501 tcgccgtctg tatggcaaag aggtttacca tcgcccgtcg cgctttattg gcgagttgcc
    16561 ggaagagtgt gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca
    16621 gcggatgggt acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgtgtacg
    16681 ccacgctaag tttggtgaag gcaccatcgt caatatggaa ggcagcggtg aacatagccg
    16741 tttgcaggtg gcattccagg gccagggaat caaatggctg gtggcagctt acgcccggct
    16801 ggagtcggtg taacgttgcc ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat
    16861 tcagattcaa taaattgcaa cgtcatgtag gcaggataag gcgtttacgc cgcatccggc
    16921 atcagcagca tatttagtat ctgacacaaa gctatcattc taacctttcc gctcgacgga
    16981 aatcatggtg acaaaaggat agcgttgcca cgggattgcc ccgcctttca tatacatatg
    17041 tgaaatcgtg ccaccaagat aaagcagctg ctcaacgttc agtttcgctt tggcataaca
    17101 ggcaaaatca taaaaatttg tcgcctgctg gctcaacaaa aacacggcat tcccatgttt
    17161 attaatccca acaccgttac gaattttacg tgaggcgacg ttgggatgaa tacgcggatt
    17221 aattacaccg ttttccatca acattgctcc tgactgcacc gcaaactgaa tatctttact
    17281 ggctttaaaa gcatccagac gaacgatgcc gactttatct cccgcgacat aaaacacgcc
    17341 gccaagacgg ataaagaaat tcccttcacc tgaagcgaga tttaacgcta ccttctg
//
LOCUS       DAJTYF010000077        17332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTYF010000077 DAJTYF010000000
VERSION     DAJTYF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.077"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..1139)
                     /locus_tag="OO590_002701"
     CDS             complement(<1..1139)
                     /locus_tag="OO590_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6638009.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADDIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPSCVVPKLNSSWEPVYQVTFPDGSADVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGG"
     gene            complement(1139..1321)
                     /locus_tag="OO590_002702"
     CDS             complement(1139..1321)
                     /locus_tag="OO590_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638010.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            complement(1333..2829)
                     /locus_tag="OO590_002703"
                     /pseudo
     CDS             complement(1333..2829)
                     /locus_tag="OO590_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3063..3557)
                     /locus_tag="OO590_002704"
     CDS             complement(3063..3557)
                     /locus_tag="OO590_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6638011.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEELPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3683..4033)
                     /locus_tag="OO590_002705"
     CDS             complement(3683..4033)
                     /locus_tag="OO590_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6638012.1"
                     /translation="MPPRIPKACRVRGCRSTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDVIRVRVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKEKTARERLK"
     gene            complement(4084..4416)
                     /locus_tag="OO590_002706"
     CDS             complement(4084..4416)
                     /locus_tag="OO590_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638013.1"
                     /translation="MPVNIDPEKLNDEREQVIAKWLFKDVDLISQQIELGEENVKRFD
                     ELLSIFDCCQSSWFATEHLFDNTELEKVWHEFESNFNKYINGGESKDLLMKMLDKLIS
                     SRFVFESR"
     gene            complement(4879..5271)
                     /locus_tag="OO590_002707"
     CDS             complement(4879..5271)
                     /locus_tag="OO590_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCR6638014.1"
                     /translation="MKLSYKLLIVGFFVTVIGSFIWSANHYYSKYQYEKKRADEAVRN
                     AESATAITNNVLQSLQIVNTVLEANQHAKQQIALESQRAREDIKVAVADDDCASRPVP
                     DVAADRLRKYADSIRAGSNDAVTDEPAR"
     gene            complement(5255..5731)
                     /locus_tag="OO590_002708"
     CDS             complement(5255..5731)
                     /locus_tag="OO590_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR6638015.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLALKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKEAGGVVNEAEL"
     gene            complement(5735..6061)
                     /locus_tag="OO590_002709"
     CDS             complement(5735..6061)
                     /locus_tag="OO590_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR6638016.1"
                     /translation="MPHNPNTWLELVQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            6358..7689
                     /locus_tag="OO590_002710"
     CDS             6358..7689
                     /locus_tag="OO590_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HCR6638017.1"
                     /translation="MLDTLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTG
                     KTEFSIKLKNLIIEQETESKVVYIDAFKGDHAESPLLLITSAIASILPEEEKQHFIKR
                     SLPAIRFGLKTVLKAGAGWFLRQEASEVSEEFQDAMKKASNAAIDGTIENLLEDHMES
                     EKNINSLKSCIESISESQKIVIIIDELDRCKPSFSTNVIETIKHIFDINNVFFILVTN
                     TEQLKASINHIYGYSINSQKYLDKFIKYTITLPDTCLINGHVVCKTSVIYWNYLVDKT
                     KSLNEINRLSGRFIRDLIQRTNLSLRETQTFSRNLNIFQLLNDDEYKSSDPLINKIFV
                     VAVFIHCFGDKEKLKQEITAESISHLADLLNIKEIPYSYERRSQIPEISIVFFGIIKD
                     SITLNERFAPKSDEELKKFTTVYTDYEHLNFWSTTPRELLIKYINQMSFIQ"
     gene            complement(7718..8086)
                     /locus_tag="OO590_002711"
     CDS             complement(7718..8086)
                     /locus_tag="OO590_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HCR6638018.1"
                     /translation="MMRDIQMVLERWGAWAASDSSGVDYSPIAAGFKGLLPYTSKKRL
                     ACSDSDALIIEGCLARLKQKRPYEHSLLVAHYLYRISKRKIAKARGKDEKLIRIEIQL
                     AEGFIDGCLSMLDLTLDLDV"
     gene            complement(8101..9090)
                     /locus_tag="OO590_002712"
     CDS             complement(8101..9090)
                     /locus_tag="OO590_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6638019.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(9098..9895)
                     /locus_tag="OO590_002713"
     CDS             complement(9098..9895)
                     /locus_tag="OO590_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6638020.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSQDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVSRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(9915..10304)
                     /locus_tag="OO590_002714"
     CDS             complement(9915..10304)
                     /locus_tag="OO590_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6638021.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITINKYEKTAGAAA"
     gene            complement(10301..10627)
                     /locus_tag="OO590_002715"
     CDS             complement(10301..10627)
                     /locus_tag="OO590_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR6638022.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERDLKV"
     gene            complement(10624..11277)
                     /locus_tag="OO590_002716"
     CDS             complement(10624..11277)
                     /locus_tag="OO590_002716"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCR6638023.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVLTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(11277..11771)
                     /locus_tag="OO590_002717"
     CDS             complement(11277..11771)
                     /locus_tag="OO590_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6638024.1"
                     /translation="MSMSLLNDVQKFIEAHRGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(11768..12709)
                     /locus_tag="OO590_002718"
     CDS             complement(11768..12709)
                     /locus_tag="OO590_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6638025.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(12699..12878)
                     /locus_tag="OO590_002719"
     CDS             complement(12699..12878)
                     /locus_tag="OO590_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6638026.1"
                     /translation="MRQVNRWFKEHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(13054..13605)
                     /locus_tag="OO590_002720"
     CDS             complement(13054..13605)
                     /locus_tag="OO590_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638027.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDEQYLSISKLQEHAALVYKIFCVSE
                     SSDARECAAPGAVACRDCGETNA"
     gene            complement(13649..13849)
                     /locus_tag="OO590_002721"
     CDS             complement(13649..13849)
                     /locus_tag="OO590_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6638028.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            13940..14614
                     /locus_tag="OO590_002722"
     CDS             13940..14614
                     /locus_tag="OO590_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HCR6638029.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            14787..15083
                     /locus_tag="OO590_002723"
     CDS             14787..15083
                     /locus_tag="OO590_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638030.1"
                     /translation="MLKQVYEMKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIM
                     AGVVFSLRRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQPTVS"
     gene            15359..15669
                     /locus_tag="OO590_002724"
                     /pseudo
     CDS             15359..15669
                     /locus_tag="OO590_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15666..16046
                     /locus_tag="OO590_002725"
     CDS             15666..16046
                     /locus_tag="OO590_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638031.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGH"
     gene            16112..16936
                     /locus_tag="OO590_002726"
     CDS             16112..16936
                     /locus_tag="OO590_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HCR6638032.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCIPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            17064..>17332
                     /gene="yfdR"
                     /locus_tag="OO590_002727"
     CDS             17064..>17332
                     /gene="yfdR"
                     /locus_tag="OO590_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6638033.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPD"
BASE COUNT         4435 a         4554 c         3955 g         4388 t
ORIGIN      
        1 ccaccgggac gcggattcat atcttccagg tcgcggcagt cattgggaga gtaaattccc
       61 cagttaatcc cggtggcgta ggcttcaaaa cgggacttca tatccccgcg cagtaacgcc
      121 ccggcgttaa atttggcgta ataaacgccc tgcttacttt ttcgtaccag tccggtgttg
      181 atccgctgtt cgatgcgggt cagatacggc accagtgaat agttgataaa tcccagcccc
      241 agctcttcaa tattgttgaa agtggcgcga tcggtgttct gcaccatgtg caacggcacc
      301 cggaacagac gacagatttc ttcaagctga aacttgcggg tttccaggaa ctggctgtcc
      361 tcggcgttca gcgccatcga cttccagtcc agccccatct caaggatcat cgggcggtga
      421 gcattgccaa gcccggtgtg acgctcctca aaatctttct tcaggcgctc gtaagcctga
      481 tctgacagcg tctgctctgt acgcaacaca cccgacgtca ccgcgccatt gctgaacagt
      541 ctggccccgt gctcttcggt cgctgccgcc agcgatattg cctcgcgggc ataggcgatg
      601 ggattcagcc ccaccagtcc gtccagcgtc agcgtgcgca catgccagat atcctcctgg
      661 ctcagtacat ccgcggagcc atccgggaat gtgacctgat agaccggctc ccagctactg
      721 ttaagcttcg gtaccacaca gctgggatcg acgggcagca gttcagccac ttcgccaaat
      781 gctttcactt tgtaggcgta aaagtttccc cgcaggcaca gacaggtgac caccagctcc
      841 cagaactcct gcggcgtcat atagccattg ggatgcgtgg agatcagctt atgcagacgt
      901 tcgccagtgg ctctctgctt caggctgccg ttcaggtgat acaggttgca gggcaacatc
      961 ccgaccgact ccgccagcac cctgacacag gaaaaaaccg ccgtcagtcg catggcccgc
     1021 tggctgctga tctgctttcc ggtataggtg tcgtaggaca acccgatatc atccgccagc
     1081 tctgctggcg tggtcaccgg tgcgtcactt tttcgttgaa ataatcccga aaagaacact
     1141 atttacctcc gccgacagac gactgtgtac ggtcgagata tcgcgccacc agccacgacc
     1201 agaacaggca caacgccccg gcaacaacaa accccgccgg gggataaatc agccaggcac
     1261 catacgccag caaaagcgcc cccagcacgc ccaccagagg cgcgagaatc agcatgatca
     1321 taattacctc agttaaagcg agcggatccc ataggactca atgtggtcag acagcgtgtc
     1381 ttctttctcg tacagcatgg ctctgccaac cgccataatc agcgcaactg caccgtcaat
     1441 tttgttttcc gcctgctctt tgacgggctt caccacatca tcgttacccg gaatggtttt
     1501 gccgaccacg ttgccgatac actaggtcat gatgggattg ccatcatgat gaaagcgccc
     1561 cgattcaatt gccgcttcca gctctttcat cgggtcggac atgttggtgt agttctgaat
     1621 gatagtgatg gggttcaggt cttcatcagc aaggtcatgt gacaacccgg tcgccccgaa
     1681 ggggtcgatg ggtgactcac tgaccggact gattttgttc gccgctttgg cctcctcgag
     1741 gatgtagcga taatccacct ccgcaccatc ggtaacggtc agaacgccca tttccaccca
     1801 tttctgaaag cgttcggctg tccgtcgatc ttcatttttc tcgacgctgt acaccgtgtc
     1861 atacggtacc cagaaacgcg gggccacact gtagtaatgc gttttaccgt caatctcgcg
     1921 ggtataaagt cgcgccatgc tgttcatatc cagcttacgc gccaggtcaa aggccagaat
     1981 gcacggctgc ccctcgaact gctcaagggt cagtgattta tcctcgcagc tctgccagct
     2041 caccaggttg aaatacgccg aacgcgccga cacccagata ttgaggtgtt ttgttttaaa
     2101 gacgtttgcc agacgggcgt tatttttcgc acgctgctgc tgacttaaca aaaattcgcg
     2161 ataaaccgac acgccaatat ttggattggc tttttccagc acctgcgggt cggtccagtc
     2221 gtcaccttca tcaacggtat agataatccc gaacagttca tcgttgggta ccgaaccgtt
     2281 gagcatctcg atgacttccc gccgtttgtc gtagcacggc ccctcaatgt tgtacccggc
     2341 ggtagtgata gcccacatca gtggctgacg tcgcgccccc atcccggtaa gcatcgtggt
     2401 gtaaagcgca tctgtggcgt gctcgtgata ttcatccacc accgcacagt ggggtgatga
     2461 accatcaccg gggttaccaa tcagcggttc aaaccgcgcc ccatcctccg gacggttcat
     2521 gtttgaggcg ttaacctcaa tcccgaacgc ttccgtcagc atgggtgtgc gtttacacat
     2581 cagtcgcgcc gggcgaaaga cttcccacgt ctgtttctct gtcgtggcac cggaatacac
     2641 ttccgcgcca aactcgttat cacaggcaaa acaatacagg gcaacaccgg cagagattgc
     2701 cgatttgccg ttcttacggg ggatttcggt atacacctcc cggaagcggc gcagccggga
     2761 ccctttattg acccagccaa acgcacagca gatcacaaag agctgccacg gctccagcgt
     2821 gatgggcatc ctcttgaatg cccactcccc cttggtgtgt ggcaacagct gaataaattt
     2881 cgcggcccgt tcagccagct ccttgtcgaa gcggtaacga aacgacttac ttttttccgc
     2941 catcaggtca tcaagatggc gctggcaggc ctgaatcaca aactggcagg ccacaatctt
     3001 tccgcgcacg acatcacggg catactgatt ggcagcattt acgttggggt aagatttccg
     3061 gctcatgact cgatgatttt cagaaacggg ttagtggctt tcttctgccc cgccaggcca
     3121 atcagacgct ggcggctact ggggtcgagt ccgagcattg cccccgtgct gctcatctcg
     3181 gactcctgtt cttttttggc ggtcagctcc ggatttttga ccataccgcc cattgcaccg
     3241 gtgatggtgt tgccctgtct ggcaatattt ttcacggcac gccgccagaa ctcataggcc
     3301 acacaccacc gctcaagcac cgcaaggtca gtcacgcaca gcaggccctg accgcagagt
     3361 tctttggttg tcagttgcca catgatcgtg gcgagaggaa gctcttcttc agcgaaccac
     3421 tccggtggct caacaccttt gatgggcgta aaaacaggtt catctttgtt cagggctcgc
     3481 ttgccggggt ttccggccag cgccttgcgc gccgttggct tagggcgacg cccggaacgc
     3541 cccgccgttc cagccatatg cggcactcct ggttaaattt catttttcgc gggtataaaa
     3601 aaacgatggg gcgggcagtc cggaagacgt caggtcacag agatttgacc cgcccctccc
     3661 ctcagacagt tgagaattat gatcacttta gccgttcacg ggccgtcttc tccttatggc
     3721 acggccagca caggctctgc agattgctgt cggcatctgt gccgccatgc gctttaggga
     3781 tgatgtggtc aacagttttc gcctcacgca ccacaccagc acgcagacat agctgacaca
     3841 ggcctttgtc acgcttcagg acacgcacgc ggataacatc ccacttcgaa ccataaccgc
     3901 gctgatgacg agattgtcct ggcttgtatt gcttccagcc ttcgctttta tggctttcgc
     3961 agtagcctga cgggtccgtg gttgtagagc ggcagccgcg aacacggcag gcttttggga
     4021 ttctaggggg catataaaaa atttataata agtaaaataa taaagaaaaa tactcaaagg
     4081 caattatctt gactcaaata caaatctaga agagatgagc ttatcaagca ttttcataag
     4141 taaatcctta ctctcaccac catttatata tttattaaag ttagactcga actcatgcca
     4201 aactttttct aactcagtat tatcaaataa gtgctctgtc gcaaaccatg aagactggca
     4261 acaatcgaaa atactcaaaa gttcatcaaa tcttttaaca ttctcttctc ccaactcaat
     4321 ttgctgacta ataagatcta catctttaaa tagccatttc gcaataactt gctctcgttc
     4381 atcattgagt ttctcggggt caatattgac aggcataaat aatagagctg tctttaattt
     4441 ctttaatgca ttcctaaaat ctatttttac ttttgttttc tcttgttccc gccacgtaaa
     4501 caatgccttg aacgccaaaa ccaaagtaac aatagtagct ccagcactca cccaagatgc
     4561 aatcattgcc caacaagccc actcagcagc agcacggttt gctacaagtg tctcatatgc
     4621 aatataattt tcgttcattt ttacctcact gttaatagtg agagtattgt aactaaatgc
     4681 ataataacta gaacagtcaa ataataatat ttcacctaat gaatattatt attcaaatgc
     4741 agattgtact tttgctctcc taacctacga agatcagact tatcacggtt acatagcccc
     4801 agtgctgata gcagacttac attcaaacta agactatccc cataagtcag aggattgggt
     4861 ataactggct gtggagtttc agcgagcagg ttcgtcggta acggcatcgt tggaacctgc
     4921 acgtatactg tccgcgtact tccgcaaccg gtcagcagcg acatcaggca cagggcgtga
     4981 agcacaatca tcatccgcaa cagccacttt gatatcttcc cgggctctct gtgactccag
     5041 tgcgatctgc tgttttgcat gctggttagc ctccagaact gtattgacga tttgtagtga
     5101 ttgcaggacg ttattggtaa tggcagttgc tgattcagca tttcgtacag cctcatcagc
     5161 acgtttcttt tcgtactgat atttgctgta gtagtggttg gccgaccaga tgaaagaacc
     5221 aatgacagta acgaagaaac caacgataag cagcttatag ctcagcttca tttaccaccc
     5281 caccagcctc tttaaaccgg gcaatcaaat caccgatttt atgttcatac tgaccataac
     5341 ctgcaccggg taacgacgcc cagatattgc tgcaacggtc gatagcctga cgaatatcac
     5401 cgcgatcaat catcggtaaa gcgccacgct ctttaatctg ttgcagtgcc acagcatcct
     5461 ggcttttggg ggagaagtct ttcagagcaa gctgcttacg gtaggcatcc caccagcgtg
     5521 aaagaagctg ataacgtccg gcggctgttg atttgagttt ggggtttagc gtgacaagtt
     5581 tgcgagggtg atcagagtaa tcagtaaaca gttcgccgcc aacaataaca tcataaccgt
     5641 gattacgtgt cggttgtcgc ccgttatccg ttccttctga ccatgccacc atatcgagga
     5701 aagctttacg ctgggaattt agtacctgca taaattactc cttagagcca ccaaacttat
     5761 taccgattac tctcattgca gccccacgaa tagcatcgac cccgattagc ccaacgccgc
     5821 caccaatggc aacagaaaga gatttaggcc atccgacata ctcaagagcg gatgcaaaag
     5881 tcagcgtcag agcaccacag agcaaaatct cgagcgtttt tcgtttccag cccccaccac
     5941 cgccaaaata tgcaatgcgc aagccagcca taacaatcga cataatcact gcgcccagcg
     6001 gtgtgtctcc acgccaccag ctctggacca actccaacca ggtatttggg ttatgaggca
     6061 tttgtagtta tctctcacct cgctgataca gcaggtgcaa attgagggaa catcatgtac
     6121 cgcaaatcag aagcggaaac gtcaaagagc cgaaccaatg gataactgcg ggataggcca
     6181 ggcccaacga atccccagca acaaaaacga caaaacccgc tcgacggcgg gtttaagctg
     6241 tgtggcgaag taaccactct taacagatta caagaatttt tgcggaccgc gttaatgatt
     6301 tttaatccca aagtcgtatt attcgttttt tactgtagga gttaataggt ttatattatg
     6361 ttagatacac tatctttcac tgaacgtgac gagttccaac gcagaaacat cgctgaaaat
     6421 atcatcaagt tgctaaaacc agaggcagac atttcaccac tggtaataga cggcgcatgg
     6481 ggaacaggga aaacagaatt tagtatcaaa ctgaaaaatc tcattattga gcaagaaact
     6541 gaatctaaag ttgtttatat tgatgccttt aaaggggatc atgcagaatc tccattactt
     6601 ctcataacct ctgcaattgc cagcatttta cctgaggaag aaaaacaaca cttcattaag
     6661 agatctcttc ctgcaattcg ctttggttta aaaacggtac taaaagctgg tgcaggttgg
     6721 tttttacgac aggaggctag tgaagtttcc gaagaattcc aagatgcgat gaagaaagca
     6781 agcaatgcag caatagatgg gactattgaa aatttgcttg aagaccatat ggaatcagag
     6841 aaaaacataa attcacttaa atcttgcatc gaaagcattt cagaaagtca aaagatagta
     6901 attataattg atgaattaga ccgatgtaag ccgagttttt caacaaatgt cattgaaaca
     6961 ataaaacaca tttttgacat caataatgtc ttttttattt tagttacaaa cacagaacaa
     7021 ttaaaagcat cgataaatca tatttatggt tatagtatta actcacaaaa gtatcttgac
     7081 aagtttataa aatataccat cacgcttcca gacacatgtt taataaatgg tcacgtcgta
     7141 tgtaaaacat ctgttatata ttggaattac cttgtagaca agacaaaatc attaaatgaa
     7201 attaataggt tatctggtag atttatccgt gatttaattc aacgaaccaa cttatcctta
     7261 cgtgaaacac aaactttttc acgcaatctt aacatttttc aactattaaa cgacgatgag
     7321 tacaaaagca gtgatccttt aataaataag atctttgtcg tagctgtctt catacattgc
     7381 tttggtgaca aggaaaaact aaaacaagaa attaccgcag aatctatatc tcatttagca
     7441 gatctgctta acataaaaga aataccttat tcttatgaga gaagatcgca aattcctgaa
     7501 atatctattg tattcttcgg aataattaaa gacagtatta ctcttaatga gcgatttgcc
     7561 cctaaaagtg atgaagaact taaaaaattc accactgttt atactgatta tgaacaccta
     7621 aatttttgga gtactacacc cagagagtta ttgataaaat atattaatca aatgtcattc
     7681 atccagtaaa taatacgccc ctgcatgggg cgtattatta aacgtccaaa tctaatgtta
     7741 gatctagcat ggaaaggcag ccatcaataa atccttcggc tagctgtatt tctatacgta
     7801 tcaatttctc atcctttcca cgcgcctttg caatcttacg cttggatatt ctgtataaat
     7861 aatgtgccac aagaagcgaa tgctcataag gtcttttttg ctttagacga gcaagacaac
     7921 cttcaataat taatgcatca ctatccgaac aagccaagcg tttcttgctg gtatagggaa
     7981 gaagtccctt aaacccagca gctataggtg aatagtcaac gcctgaacta tcactcgccg
     8041 cccatgctcc ccaacgctca agaaccatct gaatatcacg catcaacttt ctccaccaaa
     8101 tcaggccagc acgccaattg ccagcgcacg atcgataaaa cgaaatatca gctccagctg
     8161 ggagccatac ttctcttcaa atgccacggt atccgcatgc agctcgtcgt gatgctttct
     8221 gcacaaaggc aacacaaaga ggtcatgcgc ttttgtaccc attcccccct gaccgtggcc
     8281 tatcaggtgg tggggatcat cagcgggctt tccacaacat gcacacggct gtgtcttaac
     8341 ccagcgcgtg tacttctcat taacccagcg gcgacgtttt gggcgtaaca taaaagactc
     8401 cggcgactcc ggatccactt tcagcgccag cacctttttt gccttatctt ggatgatgct
     8461 ggtggcagga accgaaggca caaggtcact ttcccgggtg acagacggca caacaggctt
     8521 tggtaatctc agtgccttac gggctgcact ttccggtaag gcatccgcca ggtcattacg
     8581 aatcagccac cagcacagtt ccggcattgt cacaacgtga ctatcatcaa aaccgagatc
     8641 ccgacgcaca acagacaaca cccagcgggc acagttatcc gttgccattg attccagccg
     8701 ttccgtgaac tgatcgcgca gctggttatc acagtgccag cacagacgga ttgcgcccgg
     8761 agcgtgtcgc attgtggtca tgttctcgct gtgccagtcg gaatgaggcc actggcagcc
     8821 tttttcacga agtagccagc tttcaagaca ttccacgcca ccagcacgac ggatcactgc
     8881 ctcattgcgg aacacggccc gaacagcagg atcatccgcc agcggttgtg atgccgcggg
     8941 aacggcacca ctggcgaaag atgaataacg ttccggctca ggctccagca gtacacgccc
     9001 ctgcataaac aggggcatca gctctgaacc tggtctgaac aatacgatcc ccatacgcgg
     9061 ggcaatttca ggggtcagta gtgctctcac ggtcacctca atgaacggta tcgagcagct
     9121 ttaacagctc agggaatcgg gattcgaaga aatgcggctg cgtctcgcgc ggatttgcag
     9181 gactggtaat gttcttgccg aacatgcagc ctttcgccgt cagcgaccag aattttttga
     9241 tgttgttaat cgcggtacgg ctgtatcgtt cgcgttgttc aacgatcccc agcttcgcca
     9301 tctggtgata tgcctgatta gctgtcaggc ggataccata ctgtttcagc agtgcactca
     9361 gcgacagcgt agggcgactt gagccatcag gcgcgtcagc aggagcatca atggcatagc
     9421 gtggtgccag attcggtaag ccaacagcct cctggagttt ctgacaggcc ccaagcacag
     9481 atgagttaga caggtttaac tcccgccgca taaagtccag cagaatcacg ccagcctgca
     9541 tcttgtcagc agcctgcccg gataattttt ccggtgcgct ggttaccata tcgaaagtac
     9601 ggatcacctt cagatggaat gacgggctga tccacattgc ataggcatac accagttctt
     9661 tgcagacata cgtcccctgg ttatttccgc cacgactaac gttaactggc tctatattga
     9721 ccgagttgca aatctgcaac tcgcttatta aacgttcggt ttgctcattg cggagccaga
     9781 atgcaggctt atgcttatcc tgagaaccgg cagccctgtg cagatcgttc aggctgtaac
     9841 gcccataagc atcacgacga acttcaatac catcaatgac catcagatta ttcatacttc
     9901 gtttctcctc ttaatcaggc ggctgcaccc gccgttttct cgtacttatt gatagtgatc
     9961 tcgaccttcc cttccgggat aaccggcccc cactccacca gcattctttt cacctgactg
    10021 tcgtcttccc acacacccgc gtgggtcagg gcgtcaaaca gcgccttgtt atagttgtcc
    10081 agatcgcgga tccggttatc cggaggaaac aacacgatct ccactgaagc aggtgccgac
    10141 gttggtttcg gcagacgacg taactgctca actattgctg cgcacgccgc gctctggaat
    10201 tttcgccccg ccgcgcttat caggctctta ccagcaaacg cccctttgtt ggggtgtcgc
    10261 cagtacgtgt tcacgctggg cggaaaaggc aggatcagct tcatactttc aggtccctct
    10321 catgtaacca gtgggttgca cgcagccttg cgttttcctc accggcaagc agtgcgcgga
    10381 taatcccgac cgcctcgctg tcgtcgtcct tcaccgcggt atgaagcgtt atcccccggg
    10441 ccacgccacg ctttatcgtg atgacgcctt ttttctccag tgcgcgaaga tgctccaccg
    10501 ctgcattcac cgaacggtat cccagcatgg ttgccacctc ctgattggtt ggcgggaagc
    10561 cacgttcttt ctgataagaa atcagcatat ccagcacctg ctgctggcat tgagttaacg
    10621 tcgtcatgcc gccatctccc tgaccagttt ttccgcctgc tggcgaacct gcgccagaaa
    10681 cgcctcacca catgcctcaa gttcatcgcg cccgatgtag ctgattgccg gtcccttcca
    10741 ggtcttgtca aaaacagcaa tagcaccagc gaaaaaagct cctgtcagta cctgcttctc
    10801 gtctttcggg ataaaccaga caggcagttc aaaaccaata cgcccgcgaa taaaagcaat
    10861 atgatctgca tcttccggcc accacacttc gctggtggca gctttgatca ggaaaacata
    10921 gcgcccgccc ttatcacgca tggcactggc atgtttcatg atgtaacgca tgccggtgat
    10981 gtattgcccc tcatgctgac tggcgcggct gtatggggga ttaccaaagg cagcaccttt
    11041 aagctccgca agacgttctg accagtcatg cgccagcgcg ttgtcttccg ccgtgtaata
    11101 cgcggcacat ttggcgttat caccgtcagt gaacagatcc agaacaaacg ggccaaacag
    11161 ggtgttaatt ccccagaaaa tgttgtccgg cgtgcgccac tgatcgccca cttccttcag
    11221 ttcatgggct ggtttgttcc gcagttccac cagcgcctgg caatatttat tactcattaa
    11281 gcccccacgt aattccctga cagataccac tcttcacccg atgcagcgcg cttgctgctt
    11341 ttccgtaagc accgctcacg acgcgccaga aaattgtttc gttctggctg ggagtggctt
    11401 tcacggaatg ccgccatcca cacggttgca gcacgacggt ataagcccct ggactccagt
    11461 tcttccgcct ggcgggtcag gcacaaaatc acacggggat cgttagtgcc gacatagaaa
    11521 ttgcgcacag gtctggtttc acgaactggt tgtggttccg gttcctgcgc tctctcagtc
    11581 aggcgcggga aatgtctgcg tgtatctcct tcacaacggt gagccacacg cccactctga
    11641 cgtaacttgc ttgctgactg cagaacgcgc tgccgtgagt aaccggcaaa agcatccgca
    11701 atatctccgg aagtacagcc ccgatgggct tcaatgaatt tctgaacgtc attcaaaaga
    11761 ctcatgctca ccccctgaat cctgccggga tttggctgta gtccacgttg tcgtaactag
    11821 ctttgaagta cgggtcttcg cgtttttcgg tgtacgtgct gacggacggc gataagcgca
    11881 gggaaagctc atcccatttt tcccgcagct tcgacgggct gagcacgtta cggcaccaga
    11941 acggatcgcg actgacgcgg ctgtacatct cgcagatttg tttatgagta cgaccatcct
    12001 gcacacacat caggcgaatt tcgtttgccc aggctgtcca gttcggttct ttgggacgaa
    12061 ccacctcgcc gtcacattcg gcggcctgct cgtacagggc gatgattttt ttccagagcc
    12121 actgtgcgca ggtcaaatca tcctgcgttc cccactggcg ctttttaggg ctgaatacaa
    12181 ccgcatcagg atggcgagtt aaaaactcct gttcagccgt ctgcgtgtcc ggttgcgaag
    12241 cgtccggacg agaagttttt ttatctgacg gatcatgttt tgattttact gacggatccc
    12301 cgccagattc tgacgggtga aaacccgctt ttttgccaga tttcgacgca tcaaattttg
    12361 acgggtcaga ttttgatgcg tcagattttg acgggtcaga atctgacagt tgagaaaatg
    12421 ccgctgcctg aagcttcgca acgttaagct gataaacatt cgacgcattg cggttaccct
    12481 ggcgacgcgc cttacgcgtt aaccagcctt ctgcttccag ccgtgcgata gccgttctga
    12541 cggtactcat tcccgcgcca atctggcggg caatggtttc aattgatggc cagcacacac
    12601 cttcgtcatt actgaaatca gccaggcggg ccataattgc cacgctggat aatttcatgc
    12661 ctgatgcagc gcaaccatcc catacatagc cggttaattt agtgctcatg accgacctct
    12721 atttccctga atttacgacg aaactgttcg agcgggctga agcactcatg ctcatagcct
    12781 tcgcggaggt agataacacg ttgtgtttcc ggctcccaac gaatgactct gacgggcact
    12841 ccgtagtgct ctttgaacca gcggttaact tgtcgcaaag gactgtctcc ttctgccggt
    12901 tgaaatcacc cacagcccac tctgcaaagc tgtgggttac aatttccctg tcacctggta
    12961 catttactgc atagcaatac tccaccttcg cttttccacc cggtacagga agcgcaatca
    13021 gttgcgagcg acggtagtgt gttgttaaac tgttcatgcg ttagtttctc cacagtcacg
    13081 acatgccacg gcgcccggag ctgcacactc gcgggcgtca ctactttctg aaacgcaaaa
    13141 gattttgtag accagtgctg catgctcctg cagcttcgaa attgagaggt actgctcatc
    13201 gttaattgct gtcttctcat gcggttccac tacaccgtct tcaattgctg aacgaatctg
    13261 ttttgaataa ctgccgatct gttcaatgac ttccagcagg cgttggttga tatcggcgtt
    13321 gtccacatcc tcgacgtcag gaagagacac aaagacgcca tttgcagact gcgccacagc
    13381 atcagcaatg aagtgagtgc caccagcacg ctgtaaaacc attgcccatc ccagcgggaa
    13441 aatctgatcg ccatctgcac gaaggcggtt gaataaagcg ttttctgtta catcgagcca
    13501 gtcagccgct tcagcgtaac cacccggcaa cgccgcgata gtttttctga cagctttcac
    13561 gtaccactca ggctgttttt ctattttcca gtgatgctta cccacggtta gcctcatcgt
    13621 tctgtggtta aaaattgaaa gtgttctgct aatctttcgg atagatatcc ggtcttaagt
    13681 cagatttcgt aattgcacct gacgtgcatt gctcaagttt tttagccagc acaaaactgg
    13741 cttttttata gccattgaaa accagccgta agtagccagg tgttgagcca acttttccgg
    13801 ccaactcgcc ctgctgttct ttggttaaag agtcccaata cgctttcata caatatgtac
    13861 ctccggtgta catgttacat gattgaaatg aacctccaag acacttgtac cttaacggta
    13921 caagggtttt aatttcgtta tgaaaacaat ccatgacatc cggcggtcta acgccagaaa
    13981 actgagagat ggtgttggcg ggaattcttc ctttgccact atgattgatc gcgagccaac
    14041 ccagaccagc aggtttatgg gagatggtgc tactaaaaat atcggtgaca gcatggcacg
    14101 acacatcgaa aaatgtttcg acctgcctgt cggatggctc gatcaagaac accagacaac
    14161 gaacatcaca aaaaaacctg atgtttcaat cactaataaa caaatcacat tagtccctgt
    14221 catatcatgg gtacaggccg gagcatggaa agaagttgga tattctgagg ttgatttgag
    14281 cacagcagaa acgtatccct gccctgtacc ctgtggggaa atgacttata tcttgcgggt
    14341 gataggtgat tcaatgattg atgagtaccg cccgggagac atgatttttg tcgatcctga
    14401 agtacctgcc tgccacggtg acgacgttat tgcattgatg cacgatacag gcgaaaccac
    14461 cttcaaaagg ttgatagaag atgggacaca gcgttatctc aaagcgttaa acccaaactg
    14521 gcctgagcct tacattaaga tcaacggtaa ttgctctata attggtacag tgattttctc
    14581 aggaaaacca agaagataca aaatcaaagc ctaatcaatg tttatgaacc tgcttcggca
    14641 ggttttttta tacttgacaa tgtacccttg ggatacataa tgtacccaag cgaaacaacg
    14701 aacaggcagg acgcccacga agtagccgcc tggggcatat gaagtccagg atgattcgtt
    14761 gagtcatgtt gtgccactag gcactcatgt taaagcaggt gtatgaaatg aaagtccaga
    14821 ttttaaacaa taactgtgaa gtcgtttggt catacgacat agccgcccct gtagatcaga
    14881 gcggcgatag ctggaccaat gggaaacatc agattatggc tggagttgtg ttctctttac
    14941 gccgtgcttt ggaacaggct gaagtatttc catcagaccc tgaatggaaa tggccttttt
    15001 ctatttgtcc aaattcggag agcacatttc agaaaattgg tcagaaagtc gcactcgaag
    15061 agcatcagcc aactgtttcc tgattttttc aggtaactcg tcggcatcgc agaaacaaca
    15121 acgctcgatc atgttgaaag ccgattcgta gaactgtttc tgctgagtgt cgctgagaca
    15181 ggaaaagagc gacgttacga tgattttatt aattgcatta tcaagttctt tttcatcaaa
    15241 agtcatttga ttttcctttt atgtatacgg gcttaaaagg ataccaccga gcctgaagtg
    15301 gtgaaaagac aggcacataa cagctaagta ttttcaacca aagagaatcc ttagagttgt
    15361 ggtgaatgcg gctcagcgca cgcgggttaa ggttgaggct gacagtcgac cttctgtgga
    15421 tacccacccg tctggtgtgc aaccttcgcc aggcaccggg aggcacccgg caccacaact
    15481 ttatgctgtg tgtagtcctg gcggtaccag tttgtaccct tgcttccggc tggtaccgtc
    15541 ctttttacaa aacagagaag agcatcaccg gacgacgggc tcataaccca atccatccgg
    15601 gcggctgcta ccgcaggtgt tcttctctgt tttgtggaga aactaatcgg ccttgcaggg
    15661 tcgatatgat gaggagcagc aaaatggcta gcgaacgcag tactgatgtg caggcattta
    15721 tcggggagct ggacggcggc gtatttgaaa ccaaaatcgg cgcagttctc agtgaagtcg
    15781 cttccggtgt gatgaacacg aaaaccaaag gtaaggtctc actcaacctg gaaatcgaac
    15841 catttgatga gaaccgtgtg aaaatcaaac acaaactctc atatgttcgc ccgactaacc
    15901 gcgggaaaat ttccgaagaa gacaccaccg aaacgccgat gtatgtcaat cgcggtggtc
    15961 gcctgactat tctgcaggaa gaccagggac aattactgac tcttgccggt gaacctgacg
    16021 gaaaactccg cgcagcaggt cattaatatc gttcttaatt aactgattat ttatctcatc
    16081 actgaatatc tttatatagt taggacttat tatgtctcag aacttagacg caaccgcaat
    16141 taatcaaatc catgccctta tttctgctca gggtgttaat gaaattatca gtaagattgg
    16201 tgccgatgct gtggcattgc ctgagaattt ccgcattcat gatctggaaa aatttaattt
    16261 aaatcgcttc cgtttccgtg gtgcgctttc cactgccagc atcgatgact ttacccgtta
    16321 ttctaaagat cttgcagatg aaggcacccg ctgctttatc gatgctgata atatgcgtgc
    16381 cgtcagtgtg cttaacctgg gtactattga tgaaccaggt cacgcagata acaccgccac
    16441 tctcaaactg aaaaagacag caccgttctc tgctctgttg tctgttaatg gcgagcgtaa
    16501 ctcccagaaa tcactggcag aatggattga agactgggcc gactaccttg tgggctttga
    16561 tgctaatggt gacaccattc aggcaacaaa agcggctgcg gcggtccgta aaatcacgat
    16621 tgaagcaaac cagaccgctg attttgaaga taatgacttc agcggcaaac gctccctgat
    16681 ggagtctgtc gaagcgaaga ccaaagacat tatgccagtg gcatttgaat ttaaatgcat
    16741 tccgtttgaa ggtctgaaag aacgtccgtt taaattacgc ctcagcatta tcactggcga
    16801 tcgtccggta ctggttctgc gcattattca gctggaggcg gtgcaggaag aaatggctaa
    16861 cgaatttcgt gatctgcttg ttgagaaatt caaggacagc aaagtagaaa cctttattgg
    16921 tactttcacc gcctgatttc attactgcaa atgcccctgc gggggcattt atggaaacgt
    16981 aatttactca ataatcgccg gatggtgagg gattcttttt accagaattc agcgcggtgc
    17041 agcgcatata cgtggagaac aaaatgtcat ttattaaaac tttttccggg aagcattttt
    17101 attatgacag gataaataaa gacgacatcg atattaacga tatcgcggtt tccctttcaa
    17161 atatctgtcg ctttgccggt catctttcgc acttctacag cgtcgcccaa catgcggttc
    17221 tttgcagcca gctggtgccg caggaatttg cttttgaagc gttaatgcat gatgcaacag
    17281 aagcgtattg ccaggacatt cccgcaccac tgaaacgcct tcttcctgac ta
//
LOCUS       DAJTYF010000078        17205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTYF010000078 DAJTYF010000000
VERSION     DAJTYF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.078"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            565..1335
                     /locus_tag="OO590_002728"
     CDS             565..1335
                     /locus_tag="OO590_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6638034.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1326..1799
                     /locus_tag="OO590_002729"
     CDS             1326..1799
                     /locus_tag="OO590_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6638035.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1906..2445
                     /gene="dnaT"
                     /locus_tag="OO590_002730"
     CDS             1906..2445
                     /gene="dnaT"
                     /locus_tag="OO590_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6638036.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPVSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2448..3185
                     /gene="dnaC"
                     /locus_tag="OO590_002731"
     CDS             2448..3185
                     /gene="dnaC"
                     /locus_tag="OO590_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6638037.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3234..3728
                     /locus_tag="OO590_002732"
     CDS             3234..3728
                     /locus_tag="OO590_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6638038.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3982..6273
                     /gene="opgB"
                     /locus_tag="OO590_002733"
     CDS             3982..6273
                     /gene="opgB"
                     /locus_tag="OO590_002733"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6638039.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6411..7433)
                     /gene="lgoD"
                     /locus_tag="OO590_002734"
     CDS             complement(6411..7433)
                     /gene="lgoD"
                     /locus_tag="OO590_002734"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR6638040.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7572..8486
                     /locus_tag="OO590_002735"
     CDS             7572..8486
                     /locus_tag="OO590_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6638041.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGILTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..10061
                     /locus_tag="OO590_002736"
     CDS             8700..10061
                     /locus_tag="OO590_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6638042.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="OO590_002737"
     CDS             complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="OO590_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6638043.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="OO590_002738"
     CDS             complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="OO590_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6638044.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12611..13900
                     /locus_tag="OO590_002739"
     CDS             12611..13900
                     /locus_tag="OO590_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6638045.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPQVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13897..15363
                     /gene="hpaE"
                     /locus_tag="OO590_002740"
     CDS             13897..15363
                     /gene="hpaE"
                     /locus_tag="OO590_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6638046.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVDDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLHQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15365..16216
                     /gene="hpaD"
                     /locus_tag="OO590_002741"
     CDS             15365..16216
                     /gene="hpaD"
                     /locus_tag="OO590_002741"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6638047.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16226..16606
                     /locus_tag="OO590_002742"
     CDS             16226..16606
                     /locus_tag="OO590_002742"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6638048.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16674..>17205
                     /locus_tag="OO590_002743"
     CDS             16674..>17205
                     /locus_tag="OO590_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6638049.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGHP"
BASE COUNT         4240 a         4553 c         4301 g         4111 t
ORIGIN      
        1 ccttaaatgt ttccagcaaa ttaatagatt aagcgaataa attatcctta gcattcactt
       61 tcatatcagc atactcctgc tgcacaacaa ctccactgca tgtgtcagaa ggttagcatc
      121 aaatgataac ctggtgaaat agcctggaga catttaaaat atctacatta agttgttaga
      181 aatacttaat gaaaaacaga aatctcattc atttaattta taattaaatg atatctcttc
      241 ccgcagaatg cgggaatcac acaatcaata tttattcaat aaaatctgat gattttaact
      301 tgttaacact aaaaaacgcg cccagctatc tattatcctg acccgtaaag gctaaacaat
      361 aaattcatta tagtgataat attcatgtga tagtttgttt gcggcgagag ataattcgct
      421 ttttatcacc gagcttgtcc caataaccag gattaatcag cgcaattttg atcttaacct
      481 accgcaaaac aaccgtgctt catgatacaa atggattctc attatctgaa caatacgcag
      541 cacgtctatg acaaagggcg agttatgcaa actgagcaac agcgagccgt aacgcggctt
      601 tgtatccagt gtggattatt tcttttgcaa catggtgcgg aaagcgcgtt ggttgatgag
      661 ctttcctcac gactgggtcg ggcgctggga atggacagcg tcgaaagttc tatctcttcg
      721 aacgccatag tgctgacaac tattaaagat gggcaatgcc tgacatcgac acgtaaaaat
      781 cacgatcgcg gcattaatat gcatgtggtg actgaagtcc agcacattgt gattcttgcg
      841 gagcatcatc tgctggatta caaaggcgta gagaaacgat ttagccaaat tcaaccatta
      901 cgttacccaa gatggctggt agccttaatg gttggccttt cttgcgcctg tttctgtaaa
      961 ctcaataacg gtggctggga tggtgccgtc atcaccttct ttgccagtac gaccgcgatg
     1021 tatatccgcc agctgctggc acaacgtcat cttcatccac agatcaactt ttgccttacc
     1081 gctttcgccg ccaccaccat ttccggattg cttttgcaac tccccacttt cagcaatacc
     1141 cccaccattg cgatggccgc cagcgttctg ctgctagtgc cgggctttcc gttgattaat
     1201 gccgtcgccg atatgtttaa aggccacatc aataccggac tggcacgctg ggcgatcgcc
     1261 agtctgctga cactggctac ctgcgtcggc gtagtgatgg cactgacgat ttggggtcta
     1321 cgcggatggg tgtgatcgaa tttctgttag cgttggcgca ggatatgatc ctcgccgcca
     1381 ttcctgcggt cggctttgcg atggtgttca acgttcccgt gcgggcgtta cgctggtgtg
     1441 cgctgcttgg ctcgataggt catggttccc gaatgatctt gatgaccagc gggttgaata
     1501 ttgagtggtc aacctttatg gcttctatgc tggtcggtac cattggtatt caatggtcgc
     1561 gctggtatct ggcgcatccg aaagtgttta ccgtggcggc cgttatccct atgttcccgg
     1621 gcatatcggc ttataccgca atgatttcgt cggtaaaaat cagccagtta ggttacagcg
     1681 aaccgttgat gattaccctg ttaaccaact ttcttacagc ttcatcgatt gttggtgcgt
     1741 tatccatcgg tctttccatt cctggattat ggttgtaccg caagcgccct cgcgtataaa
     1801 attgcctcgc ttaagcacac ggatgagaga cagcctcctc tcctccgtgt gttactataa
     1861 aagttatctc ccttctcgtt catcgttcca tatttgagaa acagtatgtc ttccagagtt
     1921 ttgaccccgg acgtcgttgg tattgacgcc ctggtacacg atcaccaaac cgttctggca
     1981 aaagctgaag gcggtgtggt tgccgtattt gctaacaatg ccccggcgtt ttatgccgtc
     2041 acgcctgcac gcctggctga actgctggcg ctggaagaaa agctagcgcg tccggtaagc
     2101 gatgtcgctc tggacgatca actctatcag gaaccgcaag ccgctcccgt tgctgtaccc
     2161 atggggaaat tcgccatgta tccggactgg caacccgatg ccgattttat ccgcctggcg
     2221 gcgctatggg gcgtggcgct aagagagccg gtgaccaccg aagaactggc ctcattcatt
     2281 gcctactggc aggcggaagg taaagtcttt caccatgtgc agtggcaaca aaaactggcg
     2341 cgcagcctgc aaatcggtcg tgccagcaac ggcggactgc cgaaacgaga tgtgaatacg
     2401 gtcagtgaac ctgacagcca aattccacca ggattcagag ggtaacgatg aaaaacgttg
     2461 gcgacctgat gcaacgcctg caaaaaatga tgcctgccca tatcaaacct gcattcaaaa
     2521 cgggtgaaga gctactggcc tggcagaaag aacaaggggt gatccgctcc gccgctctcg
     2581 aacgtgaaaa tcgggcgatg aaaatgcagc gcacctttaa ccgctccggt attcgtccac
     2641 tgcatcagaa ctgctccttt gagaactatc gcgttgagtg tgaagggcag atgaatgcgt
     2701 taagcaaagc gcgccagtat gtcgaagagt ttgacggcaa catcgccagc ttcatctttt
     2761 ctggtaagcc aggaaccggc aaaaaccatc tggcggcggc aatctgcaac gagctgctgc
     2821 tacgcggtaa atccgtattg atcatcaccg tggccgatat tatgtcggcg atgaaagata
     2881 ccttcaggaa tagcggtacc agcgaagaac aactgcttaa cgatctgagc aacgttgatc
     2941 tgctggtgat cgatgagatc ggcgtgcaga ccgaatcgaa atacgaaaaa gtgatcatca
     3001 accagatcgt cgatcgccgc tcttcttcca aacgcccaac cgggatgctg accaacagta
     3061 atatggaaga gatgaccaag ttgctgggcg aacgcgtgat ggaccgtatg cgcctgggta
     3121 acagtttatg ggtgatcttc aactgggata gctaccgtag ccgggtaacg ggtaaagagt
     3181 attaatcccc gtttctggcg gtatactgcg acacaattcc accttgagta atgatgatga
     3241 aaactattaa acatcttctg tgctgtgcca ttgccgccag cgcattaatt tccaccgggg
     3301 tgcatgctgc gtcctggaaa gatgcgctct ccagcgccgc cagcgaactt ggcaaccaaa
     3361 acagcacgac acaggaaggc ggttggtcgc tcgcgtcatt aactaacttg cttagcagcg
     3421 gaaaccaggc cttaagcgca gataacatga acaatgccgc aggcattctg caatactgcg
     3481 cgaagcaaaa gctggcttcg gtaaccgatg ccgaaaacat caagaaccag gtgctggaaa
     3541 agctgggcct gaacagtgaa gagcaaaaag aagacaccaa ctatctggac ggtattcagg
     3601 gtttgctgaa aacaaaagat ggtcagcaac taaatctgga taacatcgga acgactccgc
     3661 tggcagaaaa ggtgaaaacc aaagcctgcg atctggtgtt aaagcagggg ctgaacttca
     3721 tttcctgaac cgcgaaacgc cgcgttttag ctgttacccg gctgacgcgg ctctctcctg
     3781 cccttttccc tgctcctgga cggttttccc cctgttacac gggaagcgac cgcgatcaaa
     3841 aaagtctgtt tataaactaa ttcctaacaa catcgcattt ttttgacact agaattgcag
     3901 caatatggcg acgccattac attgtgcttc tcaaaaagct taaacgttgg ctaaacggcc
     3961 cggtttagtg aggatcgccc gttgtcagaa ctactctctt tcgccctttt tctcgcctct
     4021 gtgctgattt acgcatggaa agcgggacgt aacacctggt ggtttgcagc cacgttaacg
     4081 gtgctggggc tatttgtcgt tttaaatatc accctgtttg ccagcgacta ttttactggc
     4141 gatggtatta acgacgcggt tctctatacc ttaaccaaca gcctgaccgg tgctggcgtc
     4201 agcaaataca tcctgccggg tatcggcatt gtgctggggc tgacggcggt gttcggtgcg
     4261 ctgggctgga tcctgcgccg tcgtcgtcat catccgcacc attttggtta cagcctgctg
     4321 gcgctcttac tggcgctggg ttcggtggac gccagcccgg catttcgtca gataacggaa
     4381 ctggtgaaat cccagtcacg cgatggcgac ccggactttg cggcttatta taaagagccg
     4441 tcgaaaacta tccctgaccc gaaactcaac ctggtttata tctacggcga aagtctcgag
     4501 cggacctatt ttgataacga agctttcccg gatctcacgc ctgaactggg cgcgttgaaa
     4561 aatgaaggcc tggatttcag ccacacgcag cagctgccag gaacggatta cacgattgcg
     4621 ggcatggtgg cttctcagtg cggcataccg ctgttcgccc cctttgaagg caacgcctcc
     4681 gcctctgtct ccagcttctt cccgcagaac atctgtctgg gcgatatcct gaaaaactcg
     4741 ggttatcaga actatttcgt gcagggcgca aatctgcgtt ttgccggtaa agatgtgttc
     4801 ctgaaatcgc acggcttcga ccacttatac ggctcagaag agctgaaaag cgtggtagcc
     4861 gacccgcact atcgcaacga ctggggattc tacgacgata ccgttctcga tgaagcgtgg
     4921 aaaaagtttg aagagctttc ccgctcaggt cagcgattct cactgtttac cctgacagtc
     4981 gatacccatc acccggatgg ttttatctcg cgcacctgta accgcaaaaa atatgatttt
     5041 gacggtaagc cgaatcagtc attcagcgcg gtaagttgca gccaggagaa tatcgcgacg
     5101 tttatcaaca aaatcaaagc gtcaccgtgg tttaaagata ctgtcatcgt cgtctcttct
     5161 gaccatttag cgatgaacaa cacggcgtgg aaatacctca ataagcagga tcgcaataac
     5221 ctgttttttg tcattcgtgg cgacaagccg cagcaaaaga cgctggcagt gaagcgtaac
     5281 acgatggata acggcgcgac ggtgctggac attctcggtg gcgataacta tctcggactt
     5341 ggtcgtagca gtttatccgg gcagtcgatg tcggaaatct tcctcaatat caaagagaaa
     5401 acattggcgt ggaagccaga tatcatccgc ctgtggaaat tccctaaaga gatgaaagag
     5461 ttcaccatcg accagcagaa aaacatgatt gccttctcgg gtagccattt ccgtttgccg
     5521 ctgctgttgc gggtttcaga caaacgcgtg gaaccgctgc cggaaagcga atactcagca
     5581 ccgctgcgtt tccagctggc cgatttcgct ccacgcgaca atttcgtctg ggttgacagt
     5641 tgctacaaga tggcacaact ctgggctccg gaactggcac tttccaccga ctggtgtgtc
     5701 tcgcaggggc agcttggcgg tcagcaaatt gttcagcatg ttgacaaaac aacatggaag
     5761 agcaagacgg catttaaaga tacggtcatc gacatggcgc gttacaaagg caatgtcgat
     5821 acgctgaaga ttgttgataa cgatattcgc tacaaagccg acagtttcat ctttaacgtc
     5881 gccggtgcgc cagaagaggt gaaacagttt agcgggattt cccgtccgga gtcgtggggc
     5941 cgctggtcca acgcgcagct gggcgatgaa gtaaaaatcg agtacaagca tccgctgccg
     6001 aagaaatttg acctggtgat taccgccaaa gcatacggca ataacgccag ccgtcctatt
     6061 ccggtacgcg taggcaatga agaacaaact cttgtgctgg gcaatgaagt gaccaccacc
     6121 acactgcatt tcgataaccc aaccgatgcc gacacactgg taattgtgcc gccggaacct
     6181 gtctcaacca acgaagggaa tatcctcgga cactcgccgc gtaagctcgg gatcggcatg
     6241 gtggaaatta aagtggtaga acgtgaaggg taattcctga ctgatgtgta ggtcgcatag
     6301 ggtgcgacct acacgccttc tccggcttac aacacattat taagaaacaa aaaaaacagc
     6361 gcccaacact tccccggacg ctgcatggca actttaataa tctcgcctta tcagaaagta
     6421 attacaccct taatcaactc acgattgtta atcacatcgc gctcgtaggt ttctgccagc
     6481 gtggcgaacg gatagcgatg ggttaacatc atgtcggcag tgattttccc ttccgccatc
     6541 agtcgaccga ctttagcgaa atcttccggc gtggcgttgc ggctgcccat catcgtcgtt
     6601 tcttttttat ggaattccgg atcggagaac tgcaactcac ctttaaacag accgacaaat
     6661 accaccgtgc cgccgtgacg aatcagattc acggtattat tcatcgcatg ttgattacct
     6721 gtcgcgtcga tcactttctg cgccagcgaa ccaccaaact gcgcccgcag ctgcgcgtca
     6781 aaatcttcgg ctgacgggtc cagcacgggc aactccagac gcgttgccac atgttcacgg
     6841 cgcgccggac tggtatccgc caccaccacc tgtgcgccat cggctttagc gattgccgcc
     6901 gcgcccagac cgattggccc cgccccgacc accagcacct gctcgccggg agcaatggct
     6961 gcgcgacgca ccgcatgagc gctaatagcg aaaggttcaa tcaatgcggc cgcctgcggg
     7021 tcaataccgt ctgcgggcaa aatgttcgcc accggcacgc tcaaatactc actaaaaccg
     7081 ccatcctgat gcacgccaat gactgaaatt ttttcacagc agttggtacg cccgctttta
     7141 cacgccgggc attgctgaca ggcaacataa gggatcacag caacttgctg accattttta
     7201 agatcagcaa tatttttacc cagcccaaca atctccccac atatttcatg gcctaaaacc
     7261 cgtggataac taaaaaatgg ttgatttcca ccccaggcat gaatatcggt accgcaaatc
     7321 ccgacagact taatttttat taatgcttca ttgtcacccg gaataggtat ctcgcgttgt
     7381 ttccagacta attctttcgg ctgctggcaa attaaaacat tcatcgtaga catgattcgg
     7441 ctctccattt tttgttgcct tagatatcaa tgaaaatgaa aaatggcagc ggaacggaaa
     7501 atctgttttg tgaaaacaca cgcataaaaa tgttttaaat cgggttttaa tggactcaca
     7561 aggaggaaaa tatgagtcgt tcacaaaatt tacgccacaa tgtgattaac caggtcattg
     7621 atgatatggc gcggggtcat atcccttccc cacttccatc gcaaagtgcg ctggcggaaa
     7681 tgtacaacat cagccgcact acagtgcgtc atattctcag ccaattacgc gaatgcggca
     7741 tcttaacgca ggttggaaac gactatgtta ttgcgcgtaa acctgaccat gatgacggtt
     7801 tcgcctgtac taccgcttca atgagtgaac aaaacaaagt gtttgagcag gcttttttca
     7861 cgatgataaa ccagcgtcag ttacgcccta gggaaacttt ttctgaactg caactggctc
     7921 gggccgcggg tgtcagcccg gtggttgtaa gagaatatct tttaaaattc ggccgttata
     7981 atcttattca aagcgaaaag cgcggccagt ggagcatgaa gcaattcgat caatcctacg
     8041 ccgagcagct gtttgagcta cgcgaaatgc tcgaaacaca ttcacttcag cactttctca
     8101 acctgcctga tcatgatccc cgctggctgc aggcgaaaac catgctagaa cgtcatcggc
     8161 tactgcgtga caacatcggc aatagctttc gcatgttctc gcaacttgac agagattttc
     8221 actccttact gctttcagct gccgacaata tcttttttga tcaatcgctt gagatcatct
     8281 cagtcatctt tcattttcac tatcaatggg acgaaagcga tcttaaacag cgcaatatca
     8341 ttgccgtcga tgaacatatg accattctca gcgctctgat ttgccgcagt gaccttgatg
     8401 ccacccttgc gctccgtaac catttgaatt cagctaaaca atcaatgatt cgatccatta
     8461 atgagaacac gcgttatgct cattaagtac ggattaaaaa cagcaaacta ttagcgggtg
     8521 cacggtttta tcaaaagata aataccggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtaataa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagttcggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     9001 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     9061 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     9121 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     9181 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgcgg
     9241 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9301 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9361 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9421 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9481 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9541 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9601 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9661 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9721 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9781 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9841 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9901 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9961 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
    10021 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
    10081 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
    10141 gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc cgatgtttca
    10201 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
    10261 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10321 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10381 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10441 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10501 accgagtctt caatcaggct tttaatttca cgagccgcct gggcgctacg ctgggccaga
    10561 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10621 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10681 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10741 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10801 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10861 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10921 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10981 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    11041 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    11101 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    11161 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    11221 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    11281 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11341 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11401 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11461 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11521 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11581 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tggattgctg ctggcgaatg
    11641 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11701 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11761 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11821 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11881 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11941 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    12001 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    12061 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    12121 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    12181 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    12241 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12301 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12361 aaaatcatta atatagaaac aattaatcat cacgttaccg ggactgagct gcccccgcaa
    12421 ttcttcattt tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt
    12481 atctttaaat gtaaatagtt tgttaattag atcacattta tatcacttac ttttgccaaa
    12541 ctacgcaatc gggaataaaa gtaatcatta acatattaat gattaagatg atcgaatccg
    12601 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
    12661 tgcctggcag gaagcgttcc agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg
    12721 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
    12781 tgaaaaagta ctgagcggtg cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt
    12841 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
    12901 gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc
    12961 cattggcgaa accgtggctc tcagcaatgt cgataatctg actatctata ccgagatcaa
    13021 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cacagctgct
    13081 gagcgccctg agcgaatttg ccacgctgaa tccaggcgat gctattctgc tcggcacgcc
    13141 acaggcgcgc gtggaaatac agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc
    13201 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac
    13261 gctgccacac ccgcacggta cgctgtttgc cctcggcctg aactacgccg accacgccag
    13321 cgaactggaa tttaagccac cggaagagcc gcaggtgttc ctgaaagcgc cgaataccct
    13381 cactggcgac aaccagacct ccgtgcgccc gaacaatatt gaatacatgc actacgaagc
    13441 ggagctggtg gtagttattg gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga
    13501 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc tggaaaacta
    13561 ctaccgccct aacctgcggg tcaaaagccg cgacggactg acgccgatgc tttcaaccat
    13621 cgtgccgaaa gaggcgatcc cggacccgca taatctgacc cttcgcacct tcgtcaacgg
    13681 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
    13741 ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa
    13801 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
    13861 cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc
    13921 aacggcaaaa atgttgcagg taacgactac ttccagacta ccaatccggc aacgggtgaa
    13981 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
    14041 aaagaggcgt tcccgaaatg ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc
    14101 cgtctgggcg atctgatcga ccagaacgtg cctgagatcg ccgcgatgga aaccgcggac
    14161 acgggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt
    14221 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
    14281 atgctcaact acacgctggt gcaaccggta ggcgtttgtg cgctggtgtc gccgtggaac
    14341 gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg
    14401 gtgctgaaga tgtcggaact ctccccgctg accgccaacc gcttgggtga gctggcgctg
    14461 gaagctggta ttccggcggg cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc
    14521 gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc
    14581 gagcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggagct gggcggtaaa
    14641 tcaccggtgc tgatttttga agatgccgat attgagcgtg cgctggacgc cgccctgttc
    14701 accatcttct caatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcaacag
    14761 agcatctatc cagaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtggac
    14821 gatccgaacg atccgaatac ccaggttagg gcgcttatca gtcagcaaca ctgggaaaaa
    14881 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg
    14941 gataaactgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaacggtg
    15001 ctggcggacg tagataaccg tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc
    15061 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac
    15121 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
    15181 attgaagcag gcatggtgtt cgtcaacacc cagaacgtgc gcgacctgca ccagccattt
    15241 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
    15301 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
    15361 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcga tgtatctctc
    15421 tgaactacca ggaaaaaacc acggttgtcg ccagggtgcg atcgacgggc ataaagagat
    15481 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata ctcactggct
    15541 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
    15601 cgagctgccg cattttattc gcgacatgac ctacaactac gagggcaacc cggagttggg
    15661 gcagctaatt gccgaggaag ccttaaagct cggcgtgcgg gcaaaagcgc acaatattcc
    15721 cagcctgaaa ctggaatacg gcacgctggt accgatgcgc tacatgaatg aagacaagca
    15781 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa
    15841 gctgggcgaa gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc
    15901 cagcggttcg ttatcgcacc gctttattga cgatcagcgt gcggaagaag ggatgaacag
    15961 ctacacccgc gagttcgacc gccagatgga cgagcgtgtg gtgaagctgt ggcgcgaagg
    16021 ccagttcaaa gagttttgca atatgctgcc ggagtacgcc gactactgct acggcgaagg
    16081 caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa
    16141 ggtggagttt attaccgagc tgttcccaag ctctggcacc ggtcaggtta acgctgtttt
    16201 cccgctgccc gcgtaaggac gttttatgcc gcactttatc gtggaatgta ctgaaaatat
    16261 tcgtgaagaa gcccgcttac ctgagctgtt tgccagtgtt aacactgcac tggcggcaac
    16321 ggggattttt ccgcttgggg gaatacgtag ccgggcgcac tggatcgata cctggcaaat
    16381 ggctgatggc aagcacgatt acgcctttgt gcatatgacg ctaaaaatcg gcagcggacg
    16441 cagtctggaa agccgtcagg aagtgggcga aatgctgttt gatctgatta aaacgcactt
    16501 cgcctcactg atggagagcc gttacctagc gctttcattt gagattgcag agctgcaccc
    16561 gacgttgaat ttcaaacaga acaacgtaca cgcgttgttt aaataaattt gcaccagctt
    16621 ccaggccgga tgataaggcg ctgccgcatc cggcaatgcc acaggatatc gctatgttcg
    16681 ataaacacac ccacaccctg atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac
    16741 agatccgcgc gatctcgctg gattacccgg agatcaccat cgaagacgcc tacgccgtgc
    16801 agcgcgaatg ggttcgcctg aaaatcgccg aaggtcgcac gctgaaaggc cacaaaatcg
    16861 gtctgacctc gaaagcgatg caggcaagct cgcagatcag cgaaccggat tacggtgcac
    16921 tgctggacga catgttcttc cacgatggca gcgatatccc gaccgatcgc tttatcgtgc
    16981 cgcgcattga agtggagctg gcttttgtgc tggcaaaacc gctgcgtggg ccaaactgca
    17041 cgctgttcga cgtttacaac gccacggact atgtgatccc ggcgctggag ctgatcgacg
    17101 ctcgctgcca caacatcgat ccggaaaccc agcgcccgcg caaagtattc gacaccattt
    17161 ccgataacgc cgccaacgcc ggagtgatcc tcggaggtca tccga
//
LOCUS       DAJTYF010000079        17180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTYF010000079 DAJTYF010000000
VERSION     DAJTYF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.079"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..568)
                     /gene="rlmB"
                     /locus_tag="OO590_002744"
     CDS             complement(<1..568)
                     /gene="rlmB"
                     /locus_tag="OO590_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6638050.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTG"
     gene            complement(748..3189)
                     /gene="rnr"
                     /locus_tag="OO590_002745"
     CDS             complement(748..3189)
                     /gene="rnr"
                     /locus_tag="OO590_002745"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6638051.1"
                     /translation="MSQDPFQGREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3228..3653)
                     /gene="nsrR"
                     /locus_tag="OO590_002746"
     CDS             complement(3228..3653)
                     /gene="nsrR"
                     /locus_tag="OO590_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6638052.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(3858..5156)
                     /gene="purA"
                     /locus_tag="OO590_002747"
     CDS             complement(3858..5156)
                     /gene="purA"
                     /locus_tag="OO590_002747"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6638053.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5260..5457)
                     /locus_tag="OO590_002748"
     CDS             complement(5260..5457)
                     /locus_tag="OO590_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6638054.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5539..6543)
                     /gene="hflC"
                     /locus_tag="OO590_002749"
     CDS             complement(5539..6543)
                     /gene="hflC"
                     /locus_tag="OO590_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6638055.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6546..7805)
                     /gene="hflK"
                     /locus_tag="OO590_002750"
     CDS             complement(6546..7805)
                     /gene="hflK"
                     /locus_tag="OO590_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6638056.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7891..9171)
                     /gene="hflX"
                     /locus_tag="OO590_002751"
     CDS             complement(7891..9171)
                     /gene="hflX"
                     /locus_tag="OO590_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6638057.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9247..9555)
                     /gene="hfq"
                     /locus_tag="OO590_002752"
     CDS             complement(9247..9555)
                     /gene="hfq"
                     /locus_tag="OO590_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6638058.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9641..10591)
                     /gene="miaA"
                     /locus_tag="OO590_002753"
     CDS             complement(9641..10591)
                     /gene="miaA"
                     /locus_tag="OO590_002753"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6638059.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10584..12431)
                     /gene="mutL"
                     /locus_tag="OO590_002754"
     CDS             complement(10584..12431)
                     /gene="mutL"
                     /locus_tag="OO590_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6638060.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12441..13778)
                     /gene="amiB"
                     /locus_tag="OO590_002755"
     CDS             complement(12441..13778)
                     /gene="amiB"
                     /locus_tag="OO590_002755"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6638061.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13797..14258)
                     /gene="tsaE"
                     /locus_tag="OO590_002756"
     CDS             complement(13797..14258)
                     /gene="tsaE"
                     /locus_tag="OO590_002756"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6638062.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14230..15777)
                     /gene="nnr"
                     /locus_tag="OO590_002757"
     CDS             complement(14230..15777)
                     /gene="nnr"
                     /locus_tag="OO590_002757"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6638063.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15776..16915
                     /gene="queG"
                     /locus_tag="OO590_002758"
     CDS             15776..16915
                     /gene="queG"
                     /locus_tag="OO590_002758"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6638064.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16898..16951)
                     /locus_tag="OO590_002759"
     CDS             complement(16898..16951)
                     /locus_tag="OO590_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638065.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3883 a         4895 c         4326 g         4076 t
ORIGIN      
        1 gtccggtcat tttgctctga tagagtgtat gatccgcctc gcctgccgta ccgacgatcc
       61 agatattctc ttcctgcaac atacgcatgg tgcgcgcaag gttagtcacc cgaatcagcg
      121 gaacgctttc tgccgcgccg caggctactt ttttcgccgt agcgttgagc tgtgcggagc
      181 gatctttcgg cacaatcacc gcatgaacgc ctgcggcgtc cgcactacgc aggcacgcgc
      241 cgaggttgtg cggatcggtt acgccgtcga ggatcagcag gaacggttga tcgagcgaag
      301 cgatcagatc cggcagatcg ttttcctgat actgacgtcc tggcttcacg cgggcgataa
      361 tgccctgatg cacggcaccg tcgcttttct cgtcgagata ttggcggttt gccaactgga
      421 taaccacgcc ctgagactca agggcgtgaa tcagcggtaa cagacgttta tcttcacggc
      481 cttttaaaat aaagacttcc tgaaaacgtt caggggcgcg ctccagcagg gcctgcactg
      541 cgtggatgcc gtaaatcatt tcgctcatta atgtactcgt tgtttacgtt ttgcctgatg
      601 cactacgttt atccggccta catgatctct gcaatatatt gaatttgcgt gattatgtag
      661 gccggataag gcgttcacgc cgcatccggc atgaacaaag cgcattttgt cagcaatcta
      721 accctcttct tttaaagagg gtattgatca ctctgccact tttttcttcg ccgcacgctt
      781 cgctttggtc gctgcggcta ttttctgcgt tttcgccgat ggctttttcg cttttctcgc
      841 gtctttcttc gccgctttcg gcttcgtttt tttctcaccg cggaaggcgc tgtctggctc
      901 aaagtttacc tttttaccga cctgacgacg cttgccgcct tttttgcctg catcgccttt
      961 tttcgctttc tcgcgcgccg ttttaccgac gttgcgcggt gcgcgttcac tggagatcag
     1021 actaaagtcg attttgcgct cgtccatatt aaccgcttcg acgcgaactt ccacgcgatc
     1081 gcccaggcga taagtctggc cgctggattc ccccatcagg cgttgcccta cctggtcaaa
     1141 gcgatagtag tcattgtcca gcgaagagac atggaccaga ccatcaatga acaagtcgtc
     1201 cagacggacg aagaagccaa agccagtgac gctggaaatt acgcctttaa agacgttacc
     1261 tacctggtcg agcatgaagt cacacttcag ccagtcagcg acatcgcgcg ttgcttcgtc
     1321 ggcacgacgt tccgccatcg aacagtgctg acccagttgc aacatctctt ccatcgaata
     1381 atggtagccg ccggtttcag tggtgttgcc ctgatgcccc tgctctttcg ccagcagata
     1441 tttaatggcg cggtgcagcg tcaggtcagg ataacgacga atcggcgaag taaagtgcgc
     1501 ataggactgc aatgccagac cgaagtgacc acggttttct ggatcgtaaa tcgcctgttt
     1561 catcgagcgt agcagcatgg tttgcagcat ttctgcatca ggacggtcgg caaccgactc
     1621 cagcagttcc gcgtagtcac gcggttccgg cttgttacca cccggcagct ccagccccag
     1681 ctccgccagc actgaacgga aagaggtaat cgcttcggtg ctcggcttgt cgtgaatacg
     1741 gaacagtgcc ggttctttcg ctttctcaac gaaacgcgcc gccgagatat tcgccagaat
     1801 catgcactct tcaattaatt tgtgcgcgtc gttacgctgg gtctgttcga tacgttcaat
     1861 acggcgttca gcgttgaaaa tgaacttcgc ttcttcgctc tcaaatgaga tcccaccgcg
     1921 ttcttcacgg gctttatcca gcactttata gaggttatgc aactcttcga gatgcttaac
     1981 cagcggggcg tactgctcgc gcagatcctg atcgccctgc agaatatgcc agactttggt
     2041 gtaggtcaga cgcgcgtgag agctcatcac cgcttcgtag aatttgtagc ccgtcaggcg
     2101 gcctttcgac gaaaccgtca tctcgcacac catacacagg cggtctacct gcgggttgag
     2161 cgaacacagg ccgttagaga gcacttccgg cagcatcggg ataacctgcg aagggaagta
     2221 caccgacgtg ccacggttac gcgcttctct gtccagcggc gttggcggac gcacatagta
     2281 gctgacgtcg gcaatcgcga cccataaacg ccagccgccg ccgcgttttt tctcgcagta
     2341 aactgcatcg tcaaagtcac gggcgtcttc gccatcaatg gtgaccagcg gtaaatcgcg
     2401 caaatcgaca cggcccgctt ttgcttcttc cggcacttct tctttcagcc ccgcaacctg
     2461 ttgctcaaca gcctgcggcc agatatacgg aatttcatgg gtacgcagag cgatatcaac
     2521 cgccatgccg gtgcccatat tgtcgcccag cacttcgacg attttaccca ccgctttggt
     2581 gcggcgagtc ggacgctgag tcagttcgac tacgaccaca aagcccatcc gcgcgcccat
     2641 gatctgatcg ggcgggatta agatatcgaa gctcagacgg ctatcgtcag gaaccacaaa
     2701 gccgacgccc gcttcggtaa agtagcgacc aacaatctgg ctggtttttg gcaccagtac
     2761 gcggacaata cgcgcttcac gacgaccttt acggtcagca cccagcggct gcgccagcac
     2821 ctgatcgcca tgaatgcagg ttttcatctg ctcgctggag agatacaaat catctttacg
     2881 cccttcaacc cgcagaaagc cgtagccatc acggtggcca ataacggtac ctttcaccag
     2941 gtcgaggcgt tccggcagcg catagcactg acgacgagtg aagaccagtt gaccatcgcg
     3001 ctccatcgcg cgcaggcggc gacgcaggcc ttcaagctgc tcttcgcctt caatgtgcag
     3061 ttctaccgcc agctcatcac ggctggccgg tttttcacgt ttggttaaat gttcgaggat
     3121 aaattcccga ctagggatgg gattcgcgta tttttcagct tcgcgtccct ggaaaggatc
     3181 ttgtgacatc tcggttcctc cgttgtcatc tctgatgaag attttcgtca ctccaacagc
     3241 aataatttat aaagcggttg attctcttca accaaatcgg caagcgtgta gttatccagt
     3301 tccgtaagaa aactttgcac ggccttagaa agcgcctgtt tcaaccgaca ggcaggtgta
     3361 atgtggcaaa actcactgct gcaattcacc agcgataagg gctccagctc gcgcaccaca
     3421 tcaccaatac gtatcgcact cgccggttta cccaggcgaa tgccgccatt ttttccacga
     3481 acagcagtca cgtagccggc acgactaagt tgattgatta ttttgaccat atgattacgg
     3541 gagacgccgt agacgtcagt cacttcagaa atactggtca tccgcccttc tggcaatgac
     3601 gccatgtaga tcagcgcacg taatccgtaa tcagtgaaac tcgttaactg cacatcaacc
     3661 tcaaaaggga aatcgggaaa aaatacattt atattgatga taaaccagcc acaagctgtg
     3721 tcgctaattt atttcagttc ggggaaggaa aaaagcgagg atttaacact gtgtcggata
     3781 gcgtaatggc ttaaccgaca cagcaatgta tcacactgtt agcccggcag gcaaaatatc
     3841 tgccaggcgt accagaatta cgcgtcgaac gggtcgcgca gaatcatggt ttcagtacga
     3901 tccggaccgg tagagatgat atcgatcggc acaccggtca gctcttcaat acgcttgatg
     3961 tagttcagcg ccgcctgcgg caggccgcta cgatctttca cgccgaaagt ggattcagac
     4021 cagcccggca tggtttcgta aatcggctct acacctttcc agtcgtcagc tgccagcgga
     4081 gtggtagtca cttcgcgacc atccggcata cggtaagcca cgcagagttt aacctctttc
     4141 aggccatcca gaacgtccag tttagtcagg cagaagccag acagggagtt cagctgtacc
     4201 gcacgacgaa cggcaacggt gtccagccag ccggtacgac gacgacgacc cgtagttgca
     4261 ccgaattcgt taccctgctt gcagaggaac tcgccagttt catcaaacag ttcggccggg
     4321 aaaggacctg cacctacacg agttgagtaa gctttgagga tacccagaac gtaatcaaca
     4381 taacgcgggc ccaggccgga accggtcgcc acgccaccag cagtggtgtt ggaagaagtt
     4441 acgtacggat aagtaccgtg gtcgatatcc agcagcgtac cctgcgcacc ttcgaacatg
     4501 acgaaatcgc cacgctgacg cgcctggtcg agcaggtcag aaacgtcaac caccatagaa
     4561 gtcaggatgt cggcaacagc catcgtatca tccagaactt tctggtaatc aaccgcttca
     4621 gctttgtagt agttaaccaa ctggaagttg tgatattcca tcacttcttt cagtttttca
     4681 gcgaaggttt ctttgtcgaa aaggtcgcca acacgcagac cgcgacgtgc cactttatct
     4741 tcataagcag gcccgatacc acgaccggtg gtgccgatcg ctttcgcgcc acgcgctttc
     4801 tcacgcgcgt tatccagcgc aacgtgataa tcaaggatca gcggacatgc ttcagacagc
     4861 agcagacgct cacgaacggg gatgccacgg tcttccagtt ctttcatctc tttcatcagc
     4921 gcggccggag acagcacaac accgttaccg atgatgctgg ttacattctc gcggagaata
     4981 cctgatggaa taagatggag aacggttttt tcaccgttga ttacgagagt atggcctgcg
     5041 ttgtgaccgc cctggtagcg tacaacatat ttagcccgtt cagtcagaag atcgacgatc
     5101 ttacctttac cttcgtcacc ccattgggtg cccagtacga cgacgttgtt acccattttt
     5161 caaaatcacc gtttgcttaa aaatggattc taccatcgct ttttcagagt tacagcactt
     5221 tttgcactca aaaatgacct cgaacagtct gttttttgtt cagccaatcg ttttcctcaa
     5281 catgtagtag accacaacgc ccgcaaccac aagtccaccg ccaaaacgac gtaaaatatt
     5341 atcgggcaaa ttggtcatcg cagagatcat cttcttccat gccttcgggt aaagcatcgg
     5401 ccctaaacct tccagtacca aaaccagggc aagcgccagc cagattgtcg aattcattca
     5461 ttatccttat agaaaaagaa aaccaccgac atccctgagg atgcggtggc tttattgacc
     5521 tgtaccgcag tcgttatatt aacgcgttgc ggaagtcggc gtcttcatgt agcggaagaa
     5581 atcgctatcc gggctcatga ccatcacgtc ctgattgcca gagaagctgt tctcataagc
     5641 acgcaggcta cggatgaatg cgtagaagtc cggatcttta ctgaatgcat cagcaaacag
     5701 tttggctgct tcggcatcgc cttcaccacg cataatgcgg ccctgacgct cagcttctgc
     5761 cagcgttctg gtcacttcat agtcggcagt cgcgcgcagt ttttccgctt cttcctgacc
     5821 ttgtgaacgg tgacgacgcg ctaccgcttc acgctcggcg cgcatacggt tgtagatcgc
     5881 ttcagacact tcggtcggca ggttgatctg cttgatacgc acatcgacaa cttcaatacc
     5941 cagcgccgcc atactgttcg ggttgatgac cggaactttg cccttcgtct cagccgttac
     6001 gcgctctgcc gcttcggcaa tggcgttatc tgctgccggg gtagtaactt catcttctgt
     6061 acccgcagaa ccggagttca gcgcgtcacg tacttcgagg gtcagacgac cacgggaatc
     6121 ggtgacgata tctttcacgt ccaggcgacc aatttcagaa cgcagacggt cagagaactt
     6181 acgtttcaac agcacttccg cttgcgaaat gtcgccacca cccgttgcca ggtagtaacg
     6241 gctgaaatcg ctgatgcgcc atttgatgta agagtcgacg atcaggtctt tcttctcttt
     6301 ggtcacaaag cggtcggcct ggttgtccat ggtctgaata cgtgcgtcga gcattttcac
     6361 cgtttcaatg aacggtatct tgaaatgcag accaggctca taaaccaaag gtttgttgtc
     6421 atcgtcacgc agtaccttac caaaacgcag cgtaatacca cgctcacctt ctttgacgac
     6481 aaagacagac atgtaaagca ctaccagcac gatgatgata atcgcgataa ctgacttacg
     6541 catcgttatt ccccctgacg ctggtagtcg ttacgctgcg cgttggcgcg gcgttggtcc
     6601 ataatatcgc cctgactggt ggacgacgtg ttgcttgctc cactggttgt ggaggaagag
     6661 gctggcggca gacgcagcag attgctggca ccgttatcgc tcttcgccgc aggggcgtta
     6721 ccacctttca gcatctggtc taacggcaga accatcaggt tgccaccttt atcgttaacc
     6781 agcactttgc gggtgttacc caacactttt tccatcgtct cgatatacag acgctcgcga
     6841 gtaatttccg gcgcggcttt atattccggc agaagtttag caaagcgcgc cacttcaccc
     6901 tgagcttcca ggatggtctg ggccttgtac gcacgcgcct cttcgaggat acgttgcgcc
     6961 tgaccgttcg cacgcggctg aacttcgttg gtatacgctt ctgcttcacg aatgtattgc
     7021 tgttcgtttt cacgcgcggc aatcgcatcg tcaaacgccg cttttacttc ttccggcgga
     7081 cgagcagcct ggaagttgac gtccagcagc gtgataccca tgtcatacgg acgaatcgtc
     7141 tcttccagtt cgcgctgagt atcgctacga atcacggtac gaccttccgt cagaatgcgg
     7201 tccatggtgt atttaccgat aactccacgc agggcgctgt cggtagcctg acgcaggctg
     7261 tcatccgggc tggtcacgct atacagatat ttttccggat tggtgacgcg gtactgcacg
     7321 ttcatctcaa cgcgcactac gttctcgtcc gacgtcagca tcacaccaga agcggccagt
     7381 tcacgcacgg cttccacgtt caccggtttg acttcgtcga taaacgtcgg tttccagttc
     7441 agacccggct caaccagatg gctgaattta ccaaagcgtg ttaccacgcc gcgttcggct
     7501 tctttaatgg tatagaaacc actggccgcc cagataatga caatcgctgc cgctgcgatg
     7561 gtaacgacac gaccgccaag ctgcgggcgc gggccttgcg atgaactgcc accgccagat
     7621 ccggtgcctt taccgccgcc cagaccaccg agctttttgc tcagtttgcg gaagatatca
     7681 tctaaatcag gtggcccttg atcgcgaccg cctttgtttc catttccctc agagttgccg
     7741 ccaggtttgc tgcttcccca cgggtcgcgg tcttgtccgt tattaccggg ctgattccac
     7801 gccatgtttg tgctccatat ttgttatgcg gtgatccctg ttgtctttcg acgccagagg
     7861 atatgactcc acgcttcaga cgctacgccg ttagatcagg taatcgatca acgccggttc
     7921 ttgtttacag aggcgacgcc agtcaacgat cggcatacga acttgcagac ttacgctgcc
     7981 gtcctcctcc atccactctt tttctattgc ctgaagctga taaaaacgac tcctcagacg
     8041 cccttcctgc ggtggcagac gcaatgtatg ctgcgccacc tcgccggaaa gccgctccgt
     8101 caaagcctga aaaagctgtg gtatccccgc tccggtctgt gcggaaagcc agacacggat
     8161 cggtttgttc tcttcgtccc gatcaatacg cggttcgaaa tcttccagca tatcgatctt
     8221 gttcatcacc agcagggttg ggatctcgtg agcgtcgatc tcttcaagaa ccgtattcac
     8281 cgcttcgatg ttttcttgta cacgcacatc cgccgcatca atgacgtgca gcagtaatgt
     8341 ggcttgccgc gtctcttgta acgtggcttt aaatgctgcc accagatcgt gcggcaggtg
     8401 gcgaataaac cctacggtat ctgcaagtac ggtttcaccg acatctgcaa cgtcaatacg
     8461 ccgcaacgtc gggtcgaggg tggcaaacaa ctggtctgcc gcgtagaccc gcgcttcggt
     8521 gatgcgattg aaaagggtag atttaccggc gttggtatat cccaccagcg aaacagtagg
     8581 aacgtcggct ttgatacgcg attgccgccc ctgctcacgc tgcttttcaa ctctttccag
     8641 gcgcgactgt atctgcacga tgcgattacg caacaaacga cggtcggttt cgagctgggt
     8701 ttcacccgga ccacgcaaac ctatcccgcc tttctgtctt tcaaggtggg tccagccacg
     8761 caccaggcgc gtagccagat ggcgcagctg cgccagctca acctgcaact taccctcatg
     8821 ggtacgcgca cgttgggcga aaatatctaa aataaggccg gtgcggtcga taacacgaca
     8881 ctcgcacaaa cgctccaggt tacgctcttg cgccgggctc agggcatggt caaaaagaac
     8941 gaccgaagca cccgtcgctt tgacagcttc cgcaatttca actgctttac cttcacctac
     9001 aaaatacttt gggtgcggcg ctttacggct accggtaatc acctgcaatg cttcgacacc
     9061 ggcggaagag accagagatt caaactcctg gaggtcttcc atatctttgt cttgcgtaaa
     9121 atagatgtgt accagtaccg cctgctcacc agcatcataa cggtcaaaca agcgtataac
     9181 cctctaaata gatcagcggg gaacgcagga tcgctggctc cccgtgtaaa aaaacagccc
     9241 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgtg cgctgctacc
     9301 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9361 agatgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9421 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9481 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9541 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9601 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9661 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9721 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
     9781 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
     9841 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
     9901 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
     9961 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10021 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aataaaaaaa acttccagtg
    10081 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10141 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10201 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10261 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10321 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10381 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10441 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10501 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10561 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10621 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10681 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10741 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    10801 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    10861 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc tagggcagac
    10921 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaatcagc
    10981 agcggctggg cgcaaacggg cgcttcaccc ggcgtcaatt gtgcctgacg cagccaacgt
    11041 tctgccactg gcaaggataa aagtgaaatg ttgccatcgc gctccagcaa cgcacagtcg
    11101 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11161 tgcggttccg gcgcttttgg tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11221 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11281 ctgcctgagg cgggcgcagg agagtagcgc ggagctaccg gctcacgagc tgccggttct
    11341 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggga tggcacgcgg tgcgggttgt
    11401 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11461 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11521 tgtacgttga cgtccacctg atgcgggtcg atctccagat acaacacaaa tgccggttgc
    11581 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg catgattgat caggcgatcg
    11641 cgcatcatgc gaccattcac gtagcaatac tgaatttccg ccagtgcggg cgtagtgtga
    11701 tttggatcgg ccacccagcc gcgcagggtg agatcgccgt gttgccattc aatcgccagc
    11761 gcttgttcga gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    11821 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    11881 tcgaaacgcg ccagcgcaat gcggcggatg atttcatcaa tatggctaaa ttcggttttc
    11941 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12001 gtcgttccca ccggatgcgc cgccggttta accgtcacgt ccatgtcgcg tccttcggca
    12061 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12121 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12181 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12241 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12301 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ctagttcttt gactaccgac
    12361 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12421 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12481 gcagtttgtg ccgttgcacc ctgcggcgca gattgcatcg gatgcgcaag gaagtaattg
    12541 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12601 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgatgg gatatccggc
    12661 gagcgcagaa cgccaaggct ggcgtgttct ggtcgacgtt tatgtatttc gccaatgcgt
    12721 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    12781 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatca
    12841 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttca
    12901 ctgttggcgc gacggttaga gagcaccaat acggaagcgc cagtcgcact gcggttcggt
    12961 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttatgtgc cacatcactg
    13021 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13081 tcgtcattga gcaaagtacg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13141 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13201 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13261 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13321 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggcggagga
    13381 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13441 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13501 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13561 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13621 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgtgc ctgttgatta
    13681 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13741 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    13801 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    13861 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    13921 ccttgttgtg gccactccac aaggcagatg gcatcgttgg caaaataatc gcggatcccc
    13981 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata gaccattaag
    14041 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14101 cccagagcct gtaaaaagcc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14161 agatagatta cggttgctcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14221 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14281 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14341 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcctgcac aggctgcatc
    14401 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14461 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14521 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14581 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14641 ggcctcgcta ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14701 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    14761 ggcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    14821 ttccaggctt tcggtaagag agtccatagt cagttcatgc accatcaatt ctggtcgtgc
    14881 agtcagcagc ggcgcaatgt tctcactgcg ggtcagtact cggactaaac cagcaccagc
    14941 acgtagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15001 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15061 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15121 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtgagcaa
    15181 gcctagtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15241 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15301 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15361 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15421 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15481 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15541 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15601 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15661 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15721 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    15781 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    15841 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    15901 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    15961 cgctcgcccc catgagttat tacccggtac gctgcgtgta atcagcgtgc ggatgaatta
    16021 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16081 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16141 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16201 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16261 tatcctcaat cgcgaggccg gttcgttctt ctttttaggc gaattgctgg tcgatattcc
    16321 gctgcccgtg gatcaaccag tcgaggaagg atgcggcaaa tgcgtggcct gtatgacgat
    16381 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16441 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16501 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16561 tacagaagac gatttcagcc cgcgtaagcc gctacacgca ccggaactca ttgagttatt
    16621 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16681 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcccctt gggatgaaac
    16741 gattttgaca gcgctggaaa gtcgtaaagg tgagcaccca cttctcgatg agcacatagc
    16801 gtgggcgatt gcgcagcaaa tagagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    16861 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    16921 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgaataa aatcgtcaag
    16981 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaat
    17041 accattcacc gtgcgaagtt tctttgaaat taagattaca gcctgaggct gtggataact
    17101 ctgtttacaa gagtttgtca gagacaacaa aaaccagcaa taacgcatcg cgtcgctgtg
    17161 gatattttat tgagagaaga
//
LOCUS       DAJTYF010000080        16827 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTYF010000080 DAJTYF010000000
VERSION     DAJTYF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.080"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(187..771)
                     /gene="kptA"
                     /locus_tag="OO590_002760"
     CDS             complement(187..771)
                     /gene="kptA"
                     /locus_tag="OO590_002760"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6638066.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="OO590_002761"
     CDS             1020..1703
                     /locus_tag="OO590_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR6638067.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="OO590_002762"
     CDS             1700..2161
                     /locus_tag="OO590_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6638068.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="OO590_002763"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="OO590_002763"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6638069.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTLFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="OO590_002764"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="OO590_002764"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6638070.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO590_002765"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO590_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6638071.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO590_002766"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO590_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR6638072.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5380..6210
                     /locus_tag="OO590_002767"
     CDS             5380..6210
                     /locus_tag="OO590_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6638073.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="OO590_002768"
     CDS             complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="OO590_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6638074.1"
                     /translation="MKSVTSVQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7339..8799)
                     /locus_tag="OO590_002769"
     CDS             complement(7339..8799)
                     /locus_tag="OO590_002769"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6638075.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLTLGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="OO590_002770"
     CDS             complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="OO590_002770"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6638076.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10404..11747
                     /gene="gntP"
                     /locus_tag="OO590_002771"
     CDS             10404..11747
                     /gene="gntP"
                     /locus_tag="OO590_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6638077.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="OO590_002772"
     CDS             complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="OO590_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR6638078.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="OO590_002773"
     CDS             complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="OO590_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6638079.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13436..13955)
                     /gene="fimF"
                     /locus_tag="OO590_002774"
                     /pseudo
     CDS             complement(13436..13955)
                     /gene="fimF"
                     /locus_tag="OO590_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
     gene            complement(13965..16601)
                     /gene="fimD"
                     /locus_tag="OO590_002775"
     CDS             complement(13965..16601)
                     /gene="fimD"
                     /locus_tag="OO590_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR6638080.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSIMAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQFYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(16668..>16827)
                     /locus_tag="OO590_002776"
     CDS             complement(16668..>16827)
                     /locus_tag="OO590_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone FimC"
                     /protein_id="HCR6638081.1"
                     /translation="ELNAGTRVLENALVPPMGESTVKLPSDAGSNITYRTINDYGALT
                     PKMTGVME"
BASE COUNT         4086 a         4332 c         4096 g         4313 t
ORIGIN      
        1 ggtatgaccg gaagcgacgc tgattccagc ccccaactga ccgcctgccg taaatcactt
       61 taatttcagc agttgttatt aaaggatacc cttttacgag ggtatccctt cagactgata
      121 acaaacgcta cattgccgga tgtgctgcgc ttaccaggcc tatataaccc ctgcaatata
      181 ttgaatttat tgtttttgta ccccagtcgg cataaacgac cactcaagaa actcaacagc
      241 aaccgttgat gtcagccaga ccccgttttc cgcttgccag aaagggatgc ctcgtttcgc
      301 catttcctgt gctttgacgg ttaagacaac cggcgatccg tgtcttgctc ccactttgcg
      361 tgccgttgct tcatcagcag agagatgcac gtaatgacga tcccctgcaa tcagcccctg
      421 tttttttatc tcgtcaagga aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt
      481 tttttcggca aaggaaatag cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca
      541 cctaccatca ctggaataac taaaacgctt tttatcgctg gttgcgacca cggtatccag
      601 caatgcacgg gttaatcgct tgcccgcttt ttgggcacaa agtatgagct tattgatatc
      661 cgcccatcct tcacggtcca gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa
      721 acttaaaaac ttgctcgtat ccgccagctc tttttcgttg tactttgcca tcgcgttttc
      781 gtccctggat tttctgtttt tatttactaa tgtcattgtc actgggttta tgaattttct
      841 gcaactcaat cctgcgcaga accttttcca cacagcgtaa tttttcccct atgcaaaaaa
      901 cgcatagcca gaaacatact tgcattttcc ccatacccac atggctgata aggtttttca
      961 gttccaggaa ttttcctggc tcaagcaaca cacaaacaca aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct ttttttccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc tggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccctg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattct
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacttgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggg gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagcg
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtaag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaac cgctagtata ttctgaatat tccgaatagt tacgtagatg
     4981 aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt ccgcataatc
     5041 agccatgtat tatttgaaac aattagtttg taaataatat aaattgataa catatcaaat
     5101 acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc aacatcaaat
     5161 tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa tgagcgtttt
     5221 ttgatacttt atagtgtttc ctacccttta atatcactgt gtagagttga tatcactaca
     5281 acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat ttatctattt
     5341 ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat
     5401 gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa agtcgacagt
     5461 gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta tcttctgcct
     5521 cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt
     5581 aatattggaa aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcaat
     5641 ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac
     5701 gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta
     5761 cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa
     5821 ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt
     5881 tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc
     5941 tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc
     6001 gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat
     6061 cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg tgatgaagat
     6121 gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa
     6181 aatggtgaat ggaattatat tggtaaatag ccactgagaa gaatgagtac taatattcag
     6241 gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat tgcgacttct
     6301 taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg
     6361 actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc gtcaacgttc
     6421 gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc
     6481 gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct
     6541 ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc
     6601 cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcagca
     6661 atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt
     6721 tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc
     6781 agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa
     6841 ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta
     6901 tcaagcacat agataccggc accccggcgc acttccacca gccctttgat ctccagcatg
     6961 atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc
     7021 ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc
     7081 ccgacttcct ggtaaggtct ttgcacagag gtgacagatt tcatggtgtt gtgtccgtta
     7141 ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg
     7201 gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta
     7261 caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc
     7321 tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag
     7381 ctgctgatat gccgccgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc
     7441 aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt
     7501 aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcattac cctgctcatc
     7561 cacgccctgg gtgtaacgca tccagccagc cacgcccagc gtcaggtgac gccagctgcc
     7621 gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact tctggctgcc
     7681 gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag
     7741 cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc
     7801 ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat
     7861 ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat
     7921 acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc
     7981 ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca
     8041 ggcaatggcg catgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg
     8101 cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat
     8161 ccatgccgct agttgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac
     8221 atgaccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc
     8281 acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt
     8341 ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc
     8401 gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc
     8461 gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat
     8521 aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt
     8581 tttcaggttt tcgatcagca tacggtcgtt gcctggcatc aggttaactt cgcagatgcc
     8641 ccagtcgctg tcggtgcttt ccagcagatg atgggtatac aacgcctggt gcgcgcggtg
     8701 aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg
     8761 gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct
     8821 tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtgggtat
     8881 taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct
     8941 ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc gtccagcatc
     9001 tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg
     9061 tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc
     9121 gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag
     9181 taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg
     9241 taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac
     9301 tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg cggcggatcg
     9361 tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg
     9421 aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag ctccaggtgc
     9481 ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta
     9541 cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca
     9601 atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat
     9661 gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg
     9721 aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgcca
     9781 cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca
     9841 gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca caccaggcct
     9901 gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc
     9961 gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa
    10021 gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca
    10081 catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt atccggatga
    10141 acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt
    10201 ggtaagacat gtcaacccga actctgaaat tggttaacca catcacaaga atttcactct
    10261 catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg
    10321 acattcatcg caacaatggt tgaccaattt gcatgacatg tcgcccaaaa taacaacggt
    10381 tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca
    10441 ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg
    10501 tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg ctgcacacca
    10561 tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg ttcggtgcgg
    10621 tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat acgctgctgg
    10681 cgcgtctagg tctgcgctat gttcagctgt cggtgattat catcggcctt attttcggtc
    10741 tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg
    10801 ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg
    10861 cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg
    10921 atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag
    10981 gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag
    11041 cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc
    11101 tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata
    11161 cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg tttattgcga
    11221 tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga
    11281 acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg
    11341 gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc atgttgatgt
    11401 cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg
    11461 cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc
    11521 ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg
    11581 ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg ttcaaaggtt
    11641 actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact
    11701 ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag
    11761 cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg gacagtcccc
    11821 tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg cgctttgttt
    11881 atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt
    11941 gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt aattttttta
    12001 ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc
    12061 ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta ctgctcctaa
    12121 cgataccgtg ttattcgttg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc
    12181 ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt
    12241 gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa gaggaattgg
    12301 cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcacaacc
    12361 gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact gaaaatcatc
    12421 gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac cagctttaat
    12481 cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc acggcttatc
    12541 cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg gatagctact
    12601 gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag ccgagcctcg
    12661 ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt
    12721 cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt ttacataaac
    12781 attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac aggcgaatga
    12841 ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag ctcgtttcat
    12901 tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg
    12961 ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg aagtgcgctg
    13021 attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac
    13081 tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg
    13141 tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc ggacaattag
    13201 tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata agactgaaag
    13261 aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac ggtttggcga
    13321 cgaccttacc gttcaccgtg atggtgacat cggctgcctg tatcgtcgca cttgcgagcg
    13381 ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc tccagttact
    13441 gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca ggcacctgtg
    13501 tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc agggtcgtcc
    13561 acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct
    13621 gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg ttggcattgt
    13681 ggctatctgc aacgccggta aacccaactt ttacggcaga aacagggtga cagcaaaata
    13741 cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa attgcttcgc cgcgttttcc
    13801 atcagatcaa cagtaaaatt ggttgattca gcggccacac tacagccgtt atctctgaca
    13861 tagccgcgga tagtaatcgt gctatccgca gccaaaacgc gacttaccgc cagccacaaa
    13921 aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc ccccttaacg acattcagct
    13981 gatagctggg ttaataactg ctgctgactc tctggtggca gttgataatt ggcgacacag
    14041 tgagcatttt cctcttctcc ccatttcacc tgaacttttc ccgctaaagg cattccgctg
    14101 aggtaaaact gaccattatc cgcaacaatg ccgctactct ggctactctc tgatgtcacc
    14161 atcgccccaa acggcagcgg cttattattg tgagttagcg tcatgagcag ttttatccca
    14221 acgcgcgctt taaactctgc tcgcacgatc gccccacgag tgggaacaac gttagcgacc
    14281 gcgttatcta aatcgacgtt atcagccaag gtattggtat ccagcgccac tctattttcc
    14341 cgatattcag tggcataagg cagcacggca taaccacgcc agtcggtacg cacccccgtc
    14401 tggttttcga cttttgcatc ttttgcgcca ggcgctttaa caagcaccac cgtatcgttt
    14461 aacggctgcc ccagcgttac gccattggca tgagccaata ccccaccgct gactccgtaa
    14521 tagagctgct taatatcatc gctatggctg taaccgatat tggcattgcc gtaaccaccg
    14581 cgataattca gcgtggcgta gcctgtactt ccgctattac catcgcctcc cccggcatag
    14641 ccggtttgca cgctataact gaggttgttg tcttccagca acgtaccgta tacaccagcc
    14701 agattggtca tccgaccgtt gagatcgtgt gacatgctgt agctggcact ggcatgtcgc
    14761 cactgagatt tactgtcaga acgcagccag tggctgaaag gaatattgac gttaagcgct
    14821 aacatctgat cccgtccttt ttgccaggcg tttttcgtca ggctatagct gagcgtccag
    14881 ttgatatctt cgaacgcagt atttaatcca gcctggaatt gctcatcgac attactcgtt
    14941 ccccaataag tttgatggct accactcaaa tacagtgttg atgtgcgccc gagttgctga
    15001 gtaacggtga gctgtaattt cccgcgtttg ttataagcga ggttgtaata gtcggtgaat
    15061 ttcggcttaa cctgaataac tccatcctgt gtttcgatgt tgtagccatt cattcgactg
    15121 tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat aacggtaacc cactaactga
    15181 atattcgtgc ctgattcatt gagcgatttg ttatagagaa aacgcaccga ttgtccgtca
    15241 tgctgactgt catcgggaag tgtggaatta gcctgcgtca tatccacaga cagagcgccc
    15301 agtgcctcca tgtttttccc gataccgaaa ttaaaagcac gataacgatc cgccagttgc
    15361 gttccaccat atattgtcca gccagccgga aggccgtgga gtaatgtact ctggaaaaag
    15421 cggggttttt cctgctgcgc atttccacta cggtattctc ctgccataat ggaataacga
    15481 gtatgccctt cacgttgcaa aagcgggact gacgaatagg gtacggtaaa aatctgcgtg
    15541 ctgccgtcag cctctttgat cgttacctgc aagtcaccac tattacctgc ggcatagata
    15601 tcgttgatgg taaaaggccc cggtggcacc gtactattat aaatgccata tccattttgt
    15661 ttaatagtga cctgtgcagt accatgagca ataccgtgga tcaccggggc aaatcctctt
    15721 tggctatcgg gtaacatatt gtcatctgag gccaattgtg cgccgcgaaa gttaatacca
    15781 tcgaaaatat cgccctgggt ataaccatca cccaacgtca gccgggaacg taaaggaatt
    15841 atgtctcgct caatccaggt aatgatatgc tgccatttat ttttgctacc tgatgaactg
    15901 tcgctactgt tataactcca ggtggtattg tcgcgtaaac gccacgcacc aatatttaac
    15961 ccactctgta ggtttaaata tgcataatgg ctgttacccc caatccgatt ctgtacacta
    16021 tttccgctga aattataatt gagcaatccg gcattaatac cgggatccca taactcagga
    16081 ggaatataac cacgcgcgcg attactcata aatgcctgag ggatcgtcag gttcagtcgc
    16141 tgctgaccaa catccagatg cgcagtagcg tcctggacca ttgtggttaa tggcacacag
    16201 gcatcatccg ccagcagatt cataccggcg acagaagccg tattcagccc catactggcg
    16261 agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt attaaatgtg
    16321 acatcacgcg ttgccatata accattattc aaatagatat cgacgcgata cgtccctggc
    16381 ggtaattctt gcccattttc aaaacgcgat aaatcggcca cagcctgggg atcatccgct
    16441 aaaaagcgca gattaaaata gaggtcggca gatgacaaag gtgcctgtgc ggcaaaagca
    16501 caggcgacaa cgagtcggac aaaaaaacca gccaaacgat gcttacgaat atgcaagcat
    16561 tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc ggtttaaaat
    16621 cacccggcag tcaattcttt ttattcaggc gaaaaattcc ccctgcgtta ttccattacg
    16681 cccgtcattt tgggggtaag tgcgccataa tcatttattg ttcggtaagt aatattgctt
    16741 cctgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa tgcattttca
    16801 agaacccggg ttccggcatt caactct
//
LOCUS       DAJTYF010000081        16827 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTYF010000081 DAJTYF010000000
VERSION     DAJTYF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.081"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..70
                     /locus_tag="OO590_002777"
     CDS             <1..70
                     /locus_tag="OO590_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6638082.1"
                     /translation="GNLHYIDTGAVFGGELTLVQLQ"
     gene            complement(71..262)
                     /locus_tag="OO590_002778"
     CDS             complement(71..262)
                     /locus_tag="OO590_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6638083.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(367..603)
                     /locus_tag="OO590_002779"
     CDS             complement(367..603)
                     /locus_tag="OO590_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6638084.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDARIMRLA"
     gene            complement(721..2160)
                     /gene="rsmF"
                     /locus_tag="OO590_002780"
     CDS             complement(721..2160)
                     /gene="rsmF"
                     /locus_tag="OO590_002780"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR6638085.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2240..4738)
                     /gene="yebT"
                     /locus_tag="OO590_002781"
     CDS             complement(2240..4738)
                     /gene="yebT"
                     /locus_tag="OO590_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR6638086.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTITIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLMTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKV
                     TGEMTVDPSVVTLLRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVV
                     VPGEKALLQEPDVLTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAI
                     EPQHRELVKGDSKFVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASY
                     PLYANLEKALENSLSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAF
                     DIDLHIKPEYRNLLTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGA
                     SASQRKGDKRILYASETAARAVGRQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLI
                     TARNEVQAKAVLYPEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGN
                     PRRDFELEEATITDSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLS
                     DRVMIAMRISKRYQHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATP
                     PGTPLAPKAQEGKHFLLQESEPKEWREWGTALPK"
     gene            complement(4707..5991)
                     /gene="yebS"
                     /locus_tag="OO590_002782"
                     /pseudo
     CDS             complement(4707..5991)
                     /gene="yebS"
                     /locus_tag="OO590_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6121..6618
                     /gene="msrC"
                     /locus_tag="OO590_002783"
     CDS             6121..6618
                     /gene="msrC"
                     /locus_tag="OO590_002783"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6638087.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6715..7413
                     /gene="proQ"
                     /locus_tag="OO590_002784"
     CDS             6715..7413
                     /gene="proQ"
                     /locus_tag="OO590_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6638088.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7433..9481
                     /gene="prc"
                     /locus_tag="OO590_002785"
     CDS             7433..9481
                     /gene="prc"
                     /locus_tag="OO590_002785"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6638089.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9673..10554
                     /gene="htpX"
                     /locus_tag="OO590_002786"
     CDS             9673..10554
                     /gene="htpX"
                     /locus_tag="OO590_002786"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6638090.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10600..11973)
                     /locus_tag="OO590_002787"
     CDS             complement(10600..11973)
                     /locus_tag="OO590_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6638091.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12150..12941
                     /gene="kdgR"
                     /locus_tag="OO590_002788"
     CDS             12150..12941
                     /gene="kdgR"
                     /locus_tag="OO590_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6638092.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13084..13323)
                     /locus_tag="OO590_002789"
     CDS             complement(13084..13323)
                     /locus_tag="OO590_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638093.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13482..13625
                     /gene="mgrB"
                     /locus_tag="OO590_002790"
     CDS             13482..13625
                     /gene="mgrB"
                     /locus_tag="OO590_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6638094.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13700..13987
                     /locus_tag="OO590_002791"
     CDS             13700..13987
                     /locus_tag="OO590_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6638095.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14657..14800
                     /locus_tag="OO590_002792"
     CDS             14657..14800
                     /locus_tag="OO590_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6638096.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14813..15022
                     /gene="cspE"
                     /locus_tag="OO590_002793"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14813..15022
                     /gene="cspE"
                     /locus_tag="OO590_002793"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6638097.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15188..15997
                     /gene="rlmA"
                     /locus_tag="OO590_002794"
     CDS             15188..15997
                     /gene="rlmA"
                     /locus_tag="OO590_002794"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6638098.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15994..16560)
                     /gene="mntP"
                     /locus_tag="OO590_002795"
     CDS             complement(15994..16560)
                     /gene="mntP"
                     /locus_tag="OO590_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6638099.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4429 a         4095 c         4260 g         4043 t
ORIGIN      
        1 tggcaacctg cattatattg ataccggtgc tgtctttggg ggcgaactga ctcttgtgca
       61 actgcaataa ttaaaaatca ccatactcct gtgctggtcg ccagaaacca tctataaaat
      121 cctcaatcgg aaaacaaccg ccatggcgga tccgttgatc gctcatagaa taaagacact
      181 gctgttccgt gttgtagaca tccacaacaa tatcttcaca accgccatcc aggtagcaaa
      241 caaaaagtac cagcgcgaac atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag
      301 gagagatttt acaacggggg aataaccagg acaaataacc cgccatgccg acgggttctt
      361 tttggatcag gcaagacgca taatcctggc atcagattgt tgatcgtaac gggtacggta
      421 aaggacagag tgttcgccat ttagcagggc agggatactg gtttcagcat cccaggcatc
      481 cagttgcata cataaatcgg ggtcagattt acaaggaatg gttaacgttc gatcacccgg
      541 cgattcaccg tgtaattcgc cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt
      601 catcttcgtg ctctccattt agctgctgat tgtggtacga ccagtccgat cgcacaattt
      661 ttcagcctag ttgaggccgc cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg
      721 tcatgcgtta ctggtaaaaa gtttcccgtc ccgcaccagt tcacgcgggt agctgttttt
      781 cagtcgcgaa ccaatccgtt tggctaaacc aatcggctga tgctggaaag taaccaacac
      841 gtcatccgcc actggcgcgg cttgcgggta aacatcgcgc ccgcgatacc actcctccgc
      901 ttcctgcggt gtcagttcaa aagcgtttac attgtcgggg gaggcatggg caataaccgc
      961 ttcatgctgc cagcgataac ctttgttatg cgtttcggca agtttaatcc ctaagcgaga
     1021 aaatcggact ttaccgatca gggcttcaat gcctaccggg aacaaccaca gttctttgtc
     1081 gcgttgccag agtcgcaggt ttccatccca gtttaagcca acacttgcag ccgcctgacg
     1141 aatttgtccg gcttcgcgat ctttcaccgg gctgaacggg aaattgccca ctttgtattt
     1201 tggggcgggt aaggcgggga tcgcctgagt tttacgcaga cgagcaacga agaagccttc
     1261 gcagtcgtaa atttgtggga aaacatgcaa aaaaccttct tcggtcagcg ccttgtttgc
     1321 accagggaag agatcgccaa gtggtaaaaa ctctacttcg tcggggtaag tctctttcag
     1381 ccaccggcaa acggcttcgt tttcttcccg gtttaaggta caggtcgagt aaaccagcgt
     1441 accgccagga cgtaatgcat gaaaggcgct gtcaatcagt tcccgttggg tagctgcgat
     1501 ttcctgattg ctttcgggtg accagttttt tagcgcatcg ggatctttac gcaccacgcc
     1561 ttcgccggag cagggagcgt ccagcaaaat ggcatcaaac atttctggca ctgacgcacc
     1621 aaacacgcgg ccaccaaaat gtgtgagcgc aacattactg atgccacagc ggctgatatt
     1681 ggcatgtaac acttttaccc gactggcgga aaactcattg gcaaggattg ccccttcgtt
     1741 attcatccgc gcggcaattt gcgtcgtttt tgagcctggc gcagcagcga catccatcac
     1801 ccgctgtggt gcattaccgt cagcaaacaa ggcggcgacg ggcaacattg aactggcttc
     1861 ctgaatataa aacaggccac ttaaatgctc ggtggtacta cccaatggca atgcatcttc
     1921 attgtcgcgt tcaatccaga aaccttcttc acaccacgga attggcgtaa gcgtccagcc
     1981 ataaggagcg gttaattgca ggaaatcagc aacggagatt ttcagcgtat taacgcgaat
     2041 gctgcggcgc aacgggcgct gacaggcggc aagaaaatca tcaaatgaga gcgtcgaagg
     2101 catagcttcg cgcatttgtg tcagaaacgc gtccgggaaa taaacggcgt gttgggccac
     2161 gagcatgtac cactggcaaa aaaacaggcg cgcagtttag cataaacgct ccggcgcagg
     2221 cacgccggag cagtgggcat tatttgggaa gcgcagttcc ccattcacgc cactctttcg
     2281 gttcactttc ctgcaacagg aagtgtttgc cttcctgggc tttcggtgca agtggcgtac
     2341 ccggaggcgt ggcgaaggcg atgccgccac ggataaattg gttaaaggtg ccggttttca
     2401 ctacgccgcc cgtcagacca aagtccagac tgtaacccga tgccaaccag aagacggaat
     2461 tgttacgcac caggtgttga tagcgtttac tgatgcgcat cgcaatcatc acgcgatctg
     2521 acaatgtccc cagcgtcatt ccggtaaccg tacctacttc cagaccacgg aacagcacag
     2581 gcgtaccgat gcctaacgaa ccggcttccg gcgcttcaac aataatgctt aaaccatcta
     2641 ggtaacgcga atcagtaatg gtggcctctt ctaattcaaa gtcgcgacga ggattgccac
     2701 ggcctggttc gacgttgata tacggctgga ggatagtatc tagatgctca acgccagctg
     2761 ccgagatttg cggtgtgacc actgagaagc gcgtaccacc gcgagcaaag gtctggacat
     2821 attccggata gagcaccgcc tttgcctgta cttcattgcg tgcggtaatc agatccagcg
     2881 tctggatttg cccgatatca ataccgagat agcgaattgg catcccgacc gccagttttc
     2941 cggcatcgaa agcgtgaagc gtaatctgcc tgccaaccgc acgggccgct gtttcggaag
     3001 catacagaat tcgcttgtcg cctttacgct gactggcgct ggcaccactg aggttatcga
     3061 agctaatggc cccctttaat gctctggaga gcggggatgc ctgtacggtc aggccactac
     3121 cattcagctg aactttcgcc ccgccttctg cccagaacac gctgttgctg gtcaaaaggt
     3181 tgcgatattc cggtttaatg tgcagatcga tatcgaacgc gttagctcgc ggacgcacgg
     3241 taataacttc accaacttca aatttacggt acagcactac cgatcctgcc tgcacatccg
     3301 gcagcgtctc tgcactcaaa ctcacggttg tggtgggtaa atcgctaagg ctgttctcca
     3361 gcgctttttc cagattggca tacagtggat agctggcttt catctcgcct ttatcgcccg
     3421 gcagaatacg tatcccgccg ttaatccatt ctgaggcgct ggcaccgaga aactcaacgc
     3481 catccagccc caccttcacg tcgacacggc tgttgacgac aaatttgcta tcgcctttta
     3541 ccagttctcg atgctgaggc tcgatggcga cggtaaaggt gacgcctttg ctggtgagtt
     3601 tacgatcgat aacctggcct acctgcacgc cgtgaagaat gagcggctga cccgcatcaa
     3661 taccgtaact ttccggtgct gtcagggtca gtgtcagaac gtccggctct tgcagcaggg
     3721 ctttttcgcc cggcacaaca acgaactctt tgcgtggctc gccatcgccg ggcaccaact
     3781 caaaggtttt tccggtcagc agggcgctga gattagcatc gctaagggat aatttcgggt
     3841 tgcgtaattc tatgcgggta ttttcacgaa gcagggtaac gacgctggga tcaacggtca
     3901 tttccccagt gactttacca ccaggattta aatccagttt agtcagctgt ccgacttcca
     3961 gcccctgata cattaacggc gtcgagtcgg cggttaatcc tgctccactc ggcagttcca
     4021 gtttcacctt cgcgccactg atactgacgt tggcatcaac gccggaaacg ttccagaaac
     4081 ggctaccttt tttcaccaga tcggtaaaac gccgctcgat caggacatca atcaccacgc
     4141 cttgcttgtt gggattgatg gcatagtcgt agacttttcc caccgggatc ttgcggaaat
     4201 agaccaatga accgctgttc agcgaaccga gatcgggggc ttgcaggtgg atcatcagat
     4261 cgccattgtc cagccgatat ttcggttggg tatcgagcgc gacaaagtga tcctgctctt
     4321 tacctttacc cggcatcatg ccgatatagt tcccaccgac gagggcgtcc agcccggaga
     4381 cacctgccaa cgatgctttt ggcgtcatca gccagaactg agtctcttcg cgcagcgcat
     4441 ctttcatatc ggacttgatg ctgaccttga cttcaatctt acgaagatcg tcgctgaggc
     4501 tgatatcctg cactgttccg acttcaacgc cctgataacg aacaggcgta cggcccggaa
     4561 caataccatc cgccgacata aagtcgatgg tgatggtatt accccggtcc tgataactgt
     4621 cccaaatcag ccaaccggca atcattagcg cgatgaaagg cagcagccag aaaggtgaga
     4681 tacggcgttt atttttaatc tgcgcttcag tcgtcgaagc gggcgtttcc tgactcatgt
     4741 gcatcccaaa gtaagcggct gtccagccat tccacagcaa gaatagtcaa aattacggca
     4801 gcgccaaaat aaaacgcagc cggtcccata gtaaaagcga ggatctgatc gcgattaatc
     4861 agcgacatgg ttaaagatat gacaaacagg tcgagcatcg accagcgtcc aatccaggtc
     4921 accatccgca gtaacagaat gcgtgtgcgt aaaccttgct ggcatttaaa atgaatgctc
     4981 aacagtaaag tgaacatgac gatcacttta gtaaacggta ccagaatact ggcgataaac
     5041 acgattcccg caaccgcaat gttgctactt gccagcgaca taattccgga aagaatcgta
     5101 tcttcctgcc ggccaccatt cagataaatg atagaaatag gcaataggtt ggctggtaac
     5161 aacaaaacga ttgacgctaa cagcgccgcc cagcattttt gcaaactatg acgacgacgc
     5221 aggcgtagcg ggatatggca acgcgggcag agaccacgct gatcaggata gccggtaaaa
     5281 tggcacccaa gacagacacg aagtttctcg tccctacgcg tagcggggcg ctgcggataa
     5341 tatcgctccc acagctcctc aacattaaga tgtgacaacg tcaccgtcgt taaaatcacc
     5401 aacgccacaa aagagaacaa gccgacaccc gcctggatat gggcataatc ctgtacctta
     5461 tatagaagca acgccaatgc cgaccaggta gatatccagc attacccact ctttaagtcg
     5521 ctcaagcatc agcagtaccg gacgtaaatt cattcccagt cggttaccaa accataaata
     5581 tgctatggag gacaccagaa tgaggggggc accaataacg cagaaaaaga ccattgatcc
     5641 cgttatcgca tcgccctgtt tggtcatttg ccagatgcct tgcatgacgt tggcgtcaat
     5701 acgaatgcct aacagccaga tatgcaacag cggttcgccc caggcaaacg gcataagcaa
     5761 cagcatggtg aaggccattg ccgccaggcg cgttagcgac cagtcgcgcc cgtcgcgaat
     5821 ttttgcctga cagcgcggac aataggcact ttgatgagaa tttatctcgg gcaggctaaa
     5881 cagcatgtca cattgcgggc aacgttggta atcaccacgc ggcagttcct cgccgattgc
     5941 cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg ttaagagcca tgacgtgcca
     6001 aatgtaataa gaattaacat taatcttaac ccatgatggg aatgatcttg agcattaacg
     6061 catttaatgc ttattttaat aggctggcag ttccaggtaa gacaaccaag tgattaaata
     6121 atgaacaaaa cagaatttta cgcggattta aatcgcgact ttaacgcgct gatggcggga
     6181 gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt tgttatacga gcgtctcact
     6241 gacgttaact gggcaggttt ttatttgctt gaggacgata cactggtact cggaccattt
     6301 cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg gggtgtgcgg cacagcggtt
     6361 gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt ttgacgggca tattgcctgt
     6421 gatgcggcga gtaattctga aattgttctg ccgctggtgg tgaaaaatca gattattggt
     6481 gttctcgaca tcgatagtac cgtcttcgct cgctttacag acgaggacga gcaaggctta
     6541 cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga cggattacaa aaaattcttt
     6601 gcgagcgtcg caggataatc aacggataac gtagcattta ctggtagcgt cattataatg
     6661 acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt aatcaggaaa tttcatggaa
     6721 aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt ttctcgccga acgttttccc
     6781 cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa ttggtatttt tcaggatttg
     6841 gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc aattacgatc cgctttacgt
     6901 ctctacactt cgagctggcg ttatctttac ggtgttaaac ccggcgcaac gcgtgtcgat
     6961 cttgacggca acccatgcgg tgagctggac gagcaacatg tagagcatgc tcgcaagcag
     7021 cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg aacagcaagc gaaaaaacgc
     7081 gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc gccgcgaacg caagccacgt
     7141 ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac ctcgtgcgca aaagccggta
     7201 gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac agcacacccc ggtttctgac
     7261 atttcagctc tgactgtcgg acaagccctg aaggtgaaag cgggtcaaaa cgcgatggat
     7321 gccaccgtat tagaaatcac caaagacggc gtccgcgtcc agctgaattc gggtatgtct
     7381 ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag gccgggccag gcatgaacat
     7441 gttttttagg cttaccgcgt tagctggcct gcttgcaata gcaggccaga ccttcgctgt
     7501 agaagatatc acgcgtgctg atcaaattcc ggtattaaag gaagagacgc agcatgcgac
     7561 ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat tatcgccagt tcgacctcga
     7621 tcaggcattt tcggccaaaa tctttgaccg ctacctgaat ctgctcgatt acagccacaa
     7681 tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag aaaaccgagt taggcgatga
     7741 actgcgttca ggcaaactcg acgttttcta cgatctctac aatctggcgc aaaagcgccg
     7801 ttttgaacgt taccagtacg ctttgtcggt actggaaaag ccgatggatt tcaccggcaa
     7861 cgacacttat aaccttgacc gcagcaaagc gccctggccg aaaaacgagg ctgagttgaa
     7921 cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc ctgaagctga caggaaaaac
     7981 ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa tttgccattc gtcgtctggc
     8041 gcaaaccaac agcgaagatg ttttctcgct ggcaatgatg gcgtttgcgc gtgaaatcga
     8101 tccgcatacc aactatcttt ccccgcgtaa taccgaacag ttcaacactg aaatgagttt
     8161 gtcactggaa ggtattggcg cagtgctgca aatggatgat gactacaccg ttatcaattc
     8221 gatggtggca ggtggtccgg cagcgaagag taaagctatc agcgttggtg acaaaattgt
     8281 cggtgttggt caaacaggca agccgatggt tgacgtgatt ggctggcgtc ttgatgatgt
     8341 ggttgcctta attaaagggc cgaagggcag taaagttcgt ctggaaattt tacctgctgg
     8401 taaagggacc aagacccgta ctgtaacgtt gacccgtgaa cgtattcgtc tcgaagaccg
     8461 cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa gtcggcgtgc tggatattcc
     8521 gggcttctat gtgggtttga cagacgatgt caaagttcaa ctgcagaaac tggaaaaaca
     8581 gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc ggtggggcgt tgaccgaagc
     8641 ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt gttcaggtcc gcgataacaa
     8701 cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg ttctataaag gcccactggt
     8761 ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc tttgccgcgg caatgcagga
     8821 ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt aaaggcaccg ttcagcaata
     8881 ccgttcattg aaccgtattt acgatcagat gttacgtcct gaatggccag cgctgggttc
     8941 tgtgcagtac acgatccaga aattctatcg cgttaacggc ggcagtacgc aacgtaaagg
     9001 cgtaacacca gacatcatca tgccgacggg taatgaagaa acggaaacgg gtgagaaatt
     9061 cgaagataac gcgctgccgt gggatagcat cgatgccgcg acttatgtga aatcaggaga
     9121 tttaactgcc tttgaaccgg agctgctgaa ggaacataat gcgcgtatcg cgaaagatcc
     9181 tgagttccag aacatcatga aggatatcgc gcgcttcaac gctatgaagg acaagcgcaa
     9241 tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat aatgaagatg atgcgacgcg
     9301 tctggcgcgt ttgaacgaac gctttaaacg cgaaggtaaa ccggagttga agaagctgga
     9361 tgatctaccg aaagattacc aggagccgga tccttatctg gatgagacgg tgaatatcgc
     9421 actcgatctg gcgaagcttg aaaaagccag acccgcggaa caacccgctc ccgtcaagta
     9481 atatcaatca ggcacaagaa attgtgcctg attttttaac agcggcaaga tgccgtaaat
     9541 cagatgctac aaaatgtaaa gttgtgtctt tctggtgact tacgcactat ccagacttga
     9601 aaatagtcgc gtaacccata cgatgtgggt atcgcatatt gcgttttgtt aaactgaggt
     9661 aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa cgaacctggc cgtaatggtc
     9721 gttttcgggc tggtactgag cctgacaggg atacagtcga gcagcgttca ggggctgatg
     9781 atcatggcct tgctgttcgg ttttggtggt tccttcgttt cgcttctgat gtccaaatgg
     9841 atggcattac gatctgttgg cggggaagtg atcgagcaac cgcgtaacga aagggaacgt
     9901 tggctggtca atactgtagc aacccaggct cgtcaggcgg ggatcgctat gccgcaagtg
     9961 gctatctacc atgcgccgga catcaacgct tttgcaaccg gtgcgcgccg tgatgcctct
    10021 ctggttgctg tcagcaccgg tttgctgcag aacatgagcc cggatgaagc cgaggcggta
    10081 attgctcacg aaatcagcca catcgccaat ggtgatatgg tcaccatgac gctgattcag
    10141 ggcgtggtga acaccttcgt tatctttatt tcccgtattc tggcgcagct tgccgcgggt
    10201 tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg gcaacccgct gatctacttt
    10261 gcggttgcaa cggttctgga actggtgttt ggtattctgg cgagcattat caccatgtgg
    10321 ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg caaaactggt tggtcgcgag
    10381 aaaatgattg ccgcgctgca gcgcctgaaa accagctatg aaccgcaaga agcaaccagc
    10441 atgatggctt tctgcattaa cggtaagtcg aaatcgctca gtgagttgtt catgactcac
    10501 ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg aatacctgaa gtaatatgaa
    10561 gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt tatgccctgg ctcgtggctg
    10621 agtgatacgt aaaccactga cacaggcagc aatcactgcc agaatagccg cagccatcag
    10681 cgagacgtgc gtaccattat caccaaactg atttagcatc agcgccacca gcgccgcgcc
    10741 actactctga cccagtagac gagccgttcc taacatgcca ctggccccac cgctacgttc
    10801 gcgcggagcg gaggtaataa tggtgtggtt attgggtgac tggaataagc caaatccagc
    10861 gccacataag atcatcggcc agataatatt gatatccgca ggtgacgcgg gcagcagaac
    10921 cagggaaaaa agccccgcag ccatgatgaa caaccctaaa gcacccagca atcctgcatg
    10981 tacgcgttca atcaaatagc ctgccagcgg agccatcacc atcgttgcta acggccacgg
    11041 tgtcagaagt aaacctgttt cgacttcact acgcccgagc acggtttgca ggtaaaaggg
    11101 cagggaaacc attgccagca tttgtgcgca gaaagagcaa acagatgtgc aaatagaaag
    11161 tgaaaacagc gggatacgca gtaaatccac cggtagcagc ggtacgggaa gagaaagctg
    11221 gcggcgaata aagaaaatac caacaacaac cattaccacc agttccgcag caattaacgt
    11281 cagcgattgc ccctgagcga aaccactcaa cgcagtgata agcaggccga aggttaacgc
    11341 gttcatcacg gcgctgggca ggtcgaaacg gggtttactg gcgcgagaac cattgggtgg
    11401 cagaaaacgc atcgccagaa gcagggcgat aatacctaac ggtacgttga ttaaaaataa
    11461 ccatttccag gatgcgatgg agaggattgc tgcagcaatt gtcggcccgg cagcagaaga
    11521 gacggcaaca ataaacgagt ttatgcccat ccctctcccc agaaaacgtt gtggatagat
    11581 caggcggata agtgcggtat taacgctcat caacgctgca ccgccgaaac cttgtatgac
    11641 acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg cagaacagtg aagacaacag
    11701 aaaaacgacc agaccgcatt tataaatacg tcgatagcca aacatatcgc ccagaaacga
    11761 aaacgagagc agggagatga caatggcgat ttgataggcg ttcactaccc agatggaact
    11821 ggctggtgtg gcatgaaggt ccgtggcgat tgttggcagg gcgacgttgg cgattgcgcc
    11881 gtcaaggaca gccatcgaaa taccaatcac aatggttaat atcgcaccgt atcgctgggg
    11941 caatggcagg ccgtcggcct gaacttttgg cataggaatt ttatatcttt ggtgaataat
    12001 caacaggggt atgattctag cactgatatt cacggtggat gtcgcagaat tgtaactaac
    12061 cggcaaaaga tggattgcga ccaccctggt gcgaaattat aataaaaaac agttctgatt
    12121 tttataaaac actcgcaatg aggtgataaa tggctaacgc agatctggat aaacagcctg
    12181 attctgtatc ttccgtgcta aaagtttttg gcattttgca ggcgctgggt gaagagcgcg
    12241 aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc aaaaagcacc gtttatcgct
    12301 ttttacagac catgaaaacc ttaggttatg tggcgcagga aggggagtcg gagaaatatt
    12361 ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt acaaaacgtc gatttaattc
    12421 gtagcgcaga tatccagatg cgtgagatct cccgcctgac caaagaaact atccacctcg
    12481 gcgcactgga cgaagacagt attgtttaca ttcacaaaat tgactctatg tacaatttgc
    12541 gcatgtattc acggattggg cgtcgtaatc cgctgtacag caccgcgatt ggtaaggtac
    12601 tgctggcatg gcgcgatcgc gatgaagtga agcaaattct tgagggcgtg gagtataaac
    12661 gcagtaccga gcggaccatc accagtacag aagcgttatt acccgttctg gaccaggtgc
    12721 gcgagcaggg gtatggcgaa gataacgaag agcaggaaga agggctgcga tgcattgcgg
    12781 taccggtatt tgatcgcttt ggcgtggtca ttgccggttt gagcatctcc ttcccgacgt
    12841 tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat gttgcatacc gcagcgcgca
    12901 aaatttctgc ccaaatgggt tatcacgact atccgttctg atgagagtaa gaacctgtcg
    12961 gaatatcaaa cagacaggtt ctttatttag catgagaaaa ataaagttga aggtgcgtta
    13021 tattaaacgc gcttgctata agaatatttt actcatgagt gagaatctgg ttatttatta
    13081 cccttaacca ttatcgacca cgatattgct tttacgtaac agtgggcaat ctgttatccc
    13141 caagaaacca cttttagtgt gcaagtattg taccgtgctg gtgcctctgg ccgtcagata
    13201 ggtacattgc aaacctaatc ctgcggcatt ctctttgctt ccaatcaaaa cgccatatcc
    13261 gctgagtaat aatcctatcc ataccagtgc tatcagcata actgtgcgaa tgatgaatcg
    13321 cattacaatc tcttctcttt ttatgttcgc ttaatcgtag cggcaatatg cgctgaagca
    13381 agctactcat tccgaaaaag cacgaatatc gacatagtta ggcgctgttt aactaacgca
    13441 tgctagttta atgacataag gtaggtgaag cggagattgg agtgaaaaag tttcgatggg
    13501 tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg ggcgcaggta ttcaacatga
    13561 tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc cattaaccag tttatcccgt
    13621 ggtgatatca ttttttcagc actagatttt cttcctctcc agcagtggta gaatagcgcc
    13681 tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca ggcgtgatga acattgcgtc
    13741 tttggttgta tcggtggtgg ttcttcttat cgggctcatc ttgtggtttt ttatcaatcg
    13801 tgccagttca cggactaacg aacagattga actgcttgag gcgttgctgg atcagcaaaa
    13861 acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac gaaccagaga aagcagataa
    13921 aaagaccatt gagagtcaaa aatcggttga tgacgaagat attattcgcc tggtcgccga
    13981 acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga agggttactc aattcatcct
    14041 gattatacgc actgtcatgg tgcaactgct tcaaattaaa actctggtct gacttcaaat
    14101 aattatcata ttatttattt gcgtaacggg atatcagaaa aagatatttt cctcattaat
    14161 ttattaggat gtttacatca gatttgtgat taagcgtggt attatttatt agcgaaacgt
    14221 ttctctcttg atttttttgc tcactcatca atttttctta ttttaaattt acaatccctt
    14281 tggggattga cttctcttta gggtaattga tagccgttaa ctgactgttt tatgagaaaa
    14341 agtgatataa ctttttattc attgcatagc agaaaatgtg atattgcacg cactatgtaa
    14401 taacttctcc ctctggcctg gaacaactga acttattgaa ctatgttaga aactacgcca
    14461 gtttaagtat ctgcctgaac tggcaaggtt aagcacaatg atatatcggc gcgtattccg
    14521 ttgcataagt gtgcaaaaaa agtggaagac gtatcgagat ttgtgcgtct gatcgagaca
    14581 tgtttaaaaa tggcttgcca taattaacgt tgtatgtgat aaaagatttc gggttaaacg
    14641 aggtacagtt ctgtttatgt gtggcatttt cagtaaagaa gtcctaggta aacacgttga
    14701 cgttgaatac cgcttctctg ccgagcctta tattggtgcc tcatgcagta atgtgtcagt
    14761 tttatctatg ttatgcctgc gggcgaagaa aacaatctaa ggaatttttc aaatggcaaa
    14821 gattaaaggt caggttaagt ggttcaacga gtctaaaggt tttggcttca ttactccggc
    14881 tgatggcagc aaagatgtgt tcgtacactt ctccgctatc cagggtaatg gcttcaaaac
    14941 tctggctgaa ggtcagaacg ttgagttcga aattcaggac ggccagaaag gtccggcagc
    15001 tgttaacgta acagctatct gatcgaatcc actgatctga agtgtgaata cgcttcaatc
    15061 tcgctataaa gcctcgtcga atgcgaggct ttttactatg ctttatcttc gctcctggcg
    15121 ttcggatatt tgccagccac gtgattcgcg ttacacttgc ggcctttagt atcctgccgg
    15181 agttgtcatg tcgttttcct gtccactttg ccatcagcct ctttcgcgtg aaaaaaacag
    15241 ctatatctgt ccccaacgac atcagtttga tatggcgaaa gaagggtatg tcaatctgct
    15301 gcccgttcag cataaacggt ctcgtgatcc gggcgacagc gcggaaatga tgcaagcacg
    15361 ccgcgcattc ttagatgccg gacattatca gccgctgcgt gatgcaattg tcgcccaact
    15421 gcgggaacgg cttgatgaaa aggccacggc ggtgctggat attggctgtg gtgaagggta
    15481 ttacacacac gcatttgccg atgcgttgcc cgaaatcacc acgtttggcc tggatgtctc
    15541 gaaggtagcg ataaaagcgg cggcgaaacg ctatccgcag gtcactcttt gtgtcgcttc
    15601 cagccaccgt ttgccgtttt ccgataccag tatggacgcc ataatacgta tttacgcgcc
    15661 gtgtaaagca gaagaattag cacgagtagt gaagcccggc ggctgggtca ttactgccac
    15721 gccgggaccg cgacatttga tggagctgaa ggggctgatt tacaatgaag tacatcttca
    15781 tgcacctcat gcagaacaac tggaaggttt tacattacag cagagtgcgg agttgtgtta
    15841 tccgatgcgt cttcgcggtg atgaagccgt cgcattattg cagatgacgc cgtttgcctg
    15901 gcgtgcgaag ccagaagtct ggcaaacact ggcagcaaaa gaagtgttcg actgccagac
    15961 ggactttaat attcacctct ggcagcgttc ttattaaccg tggaagtgcg tccagaggat
    16021 ctggacgccg atgccgatca gcaccagccc gccgagaatt tccgcttttt tcccaataat
    16081 tgagccgata aagcgaccaa ccatcatccc taatgtcgac ataatcaagg ttgcacaacc
    16141 aatggccaat gcggtcgcga taatgttgac ctgaaggaaa gcaagaccaa cacccacagc
    16201 catggcatcc aggctggtgg caatcgcggt ggttaccagt agccagaaac cgtgccgacg
    16261 gcgcggctct tcatcttcat catctgagcc acgaaaaccc tcaataatca ttcgcccgcc
    16321 gaggaatatc agcagcacaa acgcaatcca gtgattccat tcaaggacaa accggctggc
    16381 taacatgccc attccccagc cgatcagcgg cgtcagggtt tcgacggcac caaaaataag
    16441 gccggttcgc aatgcttcag aaaatttcgg tttatggagg ggggcacctt taccgattga
    16501 tgcagcaaat gcatccatcg acataccaaa cgcaagaaga acagtagcag tgatattcat
    16561 gacaacgtcc tgaccggggt atccataaaa cacatcatcg cccccagcaa acatgcatac
    16621 atgaacaaca agccgcgatg ggttttcgcg ttcatgcagg catagcgatt gatgtgcata
    16681 tggtctcgcc tgactgaaag tcttcagtcc gtacgtgcca cgttttgcaa cgagtatgtt
    16741 gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa
    16801 catattttga gaatataaaa caactgt
//
LOCUS       DAJTYF010000082        16683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTYF010000082 DAJTYF010000000
VERSION     DAJTYF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.082"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..2081
                     /gene="pbpC"
                     /locus_tag="OO590_002796"
                     /pseudo
     CDS             1..2081
                     /gene="pbpC"
                     /locus_tag="OO590_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2231..2662
                     /gene="ndk"
                     /locus_tag="OO590_002797"
     CDS             2231..2662
                     /gene="ndk"
                     /locus_tag="OO590_002797"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6638100.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2812..3966
                     /gene="trmG/rlmN"
                     /locus_tag="OO590_002798"
     CDS             2812..3966
                     /gene="trmG/rlmN"
                     /locus_tag="OO590_002798"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6638101.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4251..5264
                     /gene="rodZ"
                     /locus_tag="OO590_002799"
     CDS             4251..5264
                     /gene="rodZ"
                     /locus_tag="OO590_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6638102.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5291..6409
                     /gene="ispG"
                     /locus_tag="OO590_002800"
                     /gene_synonym="gcpE"
     CDS             5291..6409
                     /gene="ispG"
                     /locus_tag="OO590_002800"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6638103.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6520..7794
                     /gene="hisS"
                     /locus_tag="OO590_002801"
     CDS             6520..7794
                     /gene="hisS"
                     /locus_tag="OO590_002801"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6638104.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7812..8432
                     /gene="yfgM"
                     /locus_tag="OO590_002802"
     CDS             7812..8432
                     /gene="yfgM"
                     /locus_tag="OO590_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6638105.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8443..9621
                     /gene="bamB"
                     /locus_tag="OO590_002803"
     CDS             8443..9621
                     /gene="bamB"
                     /locus_tag="OO590_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6638106.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9739..11211
                     /gene="der"
                     /locus_tag="OO590_002804"
     CDS             9739..11211
                     /gene="der"
                     /locus_tag="OO590_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6638107.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11281..11496
                     /locus_tag="OO590_002805"
     CDS             11281..11496
                     /locus_tag="OO590_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6638108.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11493..12863)
                     /gene="xseA"
                     /locus_tag="OO590_002806"
     CDS             complement(11493..12863)
                     /gene="xseA"
                     /locus_tag="OO590_002806"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6638109.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13025..14491
                     /gene="guaB"
                     /locus_tag="OO590_002807"
     CDS             13025..14491
                     /gene="guaB"
                     /locus_tag="OO590_002807"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6638110.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14560..16137
                     /gene="guaA"
                     /locus_tag="OO590_002808"
     CDS             14560..16137
                     /gene="guaA"
                     /locus_tag="OO590_002808"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6638111.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16232..>16683)
                     /locus_tag="OO590_002809"
     CDS             complement(16232..>16683)
                     /locus_tag="OO590_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6638112.1"
                     /translation="EAEQQGKNEEQRQHDEWVAERNREIQQEKQRRANAQAAANKRAA
                     TAAANKKARQDKLDAEATADKKRDQSYEDELRSLEIQKQKLALAKEEARVKRENEFID
                     QELKHKAAQTDVVQSEADANRNMTEGGRDLMKSVGKAEENKSDSWFN"
BASE COUNT         3844 a         4170 c         4671 g         3998 t
ORIGIN      
        1 gaagcgctga tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc
       61 tcggtggcgc gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc
      121 acgctcacta tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa
      181 attctccagc tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg
      241 atcttgtatc ttaaccgcgc tccgtttggc gttacgttgc aggggatcgg tgcggcaagt
      301 tgggcttatc tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg
      361 gtgttaccgc aagtgcccag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc
      421 gcgcgtaata aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta
      481 aaagagtcaa gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg
      541 ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat
      601 gccggtcttc aacgacgtct ggaagaactg gcgcaaactg gaaagggcgg ttgccaccgc
      661 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      721 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      781 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      841 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
      901 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
      961 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1021 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1081 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1141 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1201 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1261 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1321 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1381 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1441 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1501 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1561 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1621 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1681 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1741 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1801 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1861 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     1921 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     1981 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2041 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2101 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2161 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2221 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2281 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2341 caaaatgctg cacctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2401 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2461 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2521 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2581 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2641 agtgtgcccg cgcacccgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2701 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2761 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2821 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     2881 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     2941 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3001 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3061 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3121 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3181 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3241 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3301 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3361 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3421 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3481 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3541 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3601 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3661 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3721 tgtaagatca acctgatccc gtggaacccg ttcccgggcg cgccgtatgg acgcagttcg
     3781 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3841 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     3901 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     3961 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4021 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4081 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4141 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4201 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4261 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4321 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4381 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4441 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4501 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4561 aacgccgcaa aaaacgcgac ggctggctga tgaccttcac ctggctggtg ttgtttgtgg
     4621 ttatcggcct gagcggtgcc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4681 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4741 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4801 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4861 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     4921 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     4981 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5041 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5101 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5161 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5221 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5281 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5341 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5401 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5461 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5521 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5581 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5641 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5701 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5761 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5821 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     5881 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     5941 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6001 cgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6061 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6121 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6181 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6241 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6301 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6361 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt gaaaaataat aacgtgatgg
     6421 gaagcgcctc gcttcccgtg tatgattgaa cccgcatggc tcccgaaaca ttgagggaag
     6481 cgttgagggt tcatttttat attcagaaag agaataaacg tggcaaaaaa cattcaagcc
     6541 attcgcggca tgaacgatta cctgcctggc gaaacggcca tctggcagcg cattgaaggc
     6601 acactgaaaa acgtgctcgg cagctacggt tacagtgaaa tccgcttgcc gattgtagag
     6661 cagaccccgc tattcaaacg tgcgattggt gaagtcaccg acgtggttga aaaagagatg
     6721 tacacctttg aggatcgcaa tggcgacagc ctgactctgc gccctgaagg gacggcgggc
     6781 tgtgtacgcg ccggcatcga gcatggtctt ctgtacaatc aggaacagcg tctgtggtat
     6841 atcgggccga tgttccgtca cgagcgtccg cagaaagggc gttatcgtca gttccatcag
     6901 ttgggctgcg aagttttcgg tctgcaaggt ccggatatcg acgctgaact gattatgctc
     6961 actgcccgct ggtggcgtgc gctgggtatc ttcgaacacg taactcttga gctgaattct
     7021 atcggttcgc tggaagcacg cgccaattac cgcgatgcgc tggtggcatt ccttgagcag
     7081 cataaagaaa agctggacga agactgcaaa cgccgcatgt acactaaccc gctgcgcgtg
     7141 ctggattcaa aaaatccgga agtgcaggcg cttctcaacg acgctccggc attaggtgat
     7201 tatctggacg aggaatctcg tgagcatttt gccggtctgt gcaaactgct ggagagcgcg
     7261 gggatcgctt acaccgtaaa ccagcgtctg gtgcgtggtc tggattacta taaccgtacc
     7321 gttttcgagt gggtgactaa cagtctcggc tcccagggca ccgtgtgtgc aggcggtcgt
     7381 tatgacggtc ttgtggaaca actgggcggt cgtgcaacac cggctgtcgg ttttgcgatg
     7441 ggcctcgaac gtcttgtatt gttagtacag gccgttaatc cggaatttaa agccgatcct
     7501 gttgtcgata tatacctggt ggcttcaggt gctgatacac aatctgcggc tatggcatta
     7561 gctgagcgtc tgcgtgatga attactgggc gtgaaattga tgaccaacca cggcggcggc
     7621 aactttaaga aacagtttgc ccgtgctgat aaatggggtg cccgcgttgc tgtggtgctg
     7681 ggtgagtctg aagtggctaa cggcacagca gtagtgaagg atttgcgctc tggtgagcaa
     7741 acggcagttg cgcaggatag cgtagccgcg catttgcgca cgttactggg ttaaggaagg
     7801 agaaggacag cgtggaaatt tacgagaacg aaaacgacca ggtagaagcg gttaaacgct
     7861 tttttgctga aaatggcaaa gcactggctg ttggggtgat tttgggcgtt ggcgcactga
     7921 ttggctggcg ctactggaac agccatcagg ttgattctgc acgctccgct tctcttgcct
     7981 atcaaaatgc ggttaccgca gtgagcgaag gcaaaccgga tagcatcccg gcggcggaaa
     8041 aatttgctgc tgaaaataaa aatacttatg gtgcgctggc ttctttggaa cttgcgcagc
     8101 aatttgttga caaaaatgaa ctagagaaag ctgccgccca gttacaacag gggctggcag
     8161 acacgagcga tgaaaatctc aaagccgtga taaatctgcg tcttgctcgc gttcaggtac
     8221 agctcaagca ggctgatgcc gcgctgaaaa cccttgatac catcaaaggt gaagggtggg
     8281 ctgccattgt tgccgacctg cgtggtgaag cattgctgag caaaggtgat aagcaaggtg
     8341 cgcgtagtgc atgggaagca ggcgtgaaaa gcgatgttac tccggcactg agcgaaatga
     8401 tgcagatgaa aattaataat ttgtccatct gagagggacc cgatgcaatt gcgtaaatta
     8461 ctgctgccag gactgctttc cgttaccctt ttaagcggct gttcgctgtt taacagcgaa
     8521 gaagatgtgg taaagatgtc cccattgcca accgttgaaa accagtttac gccgaccacg
     8581 gcgtggagca cttccgttgg tagcggcatt ggcaacttct attccaatct tcatccggca
     8641 ctggcggaca acgttgtcta tgcagcggac cgcgctggtt tggtaaaagc gctgaatgcg
     8701 gatgatggca aagaaatctg gtctgtcagc ctggccgaga aagatggctg gttctctaaa
     8761 gagcctgcat tactttctgg cggtgtgacc gtgtctggtg ggcatgtcta cattggcagc
     8821 gaaaaggcgc aggtttacgc gctgaatacc agcgatggta ctgtggcatg gcaaactaca
     8881 gtcgcgggtg aagcactttc gcgcccggtg gtcagcgacg gtctggtgtt aatccacacc
     8941 agtaacggtc agttacaagc gctgaacgaa gctgacggcg ctgtcaaatg gacagttaac
     9001 ctcgatatgc cttcgctctc tttgcgtggc gagtctgcgc cggcaacggc ttttggtgcg
     9061 gctgtcgtgg gtggcgataa tggtcgcgtc agcgcagtgc tgatggaaca gggccagatg
     9121 atttggcagc agcgtatttc ccaggcgacc ggttctaccg aaattgaccg tctgagcgat
     9181 gttgacacga ctcccgtcgt tgttaacggc gttgttttcg cgctggccta taatggtaac
     9241 ctgacggcgc ttgatctgcg cagtggtcag attatgtgga aacgcgaact gggttcggtg
     9301 aatgatttca tcgtcgatgg caatcgcatc tatctggtcg atcaaaatga ccgggtgatg
     9361 gcgttgacca ttgatggcgg cgttacgctg tgggcacaaa gcgatctgct gcatcgcctg
     9421 ctgacttctc cggtgctgta taatggcaac ctggtggtcg gtgacagtga aggttatctg
     9481 cactggatta acgtcgaaga tggtcgtttc gttgcccagc aaaaagttga tagttccggt
     9541 ttccagactg aaccggttgc cgctgacggc aaactgctga tccaggcaaa agacggaacc
     9601 gtgtactcta ttacacgtta atcgtctctg tcgttcactt tgaaaacggc tcctggacag
     9661 gggccgtttt cctgttttta acaacgacgc gaatatagcg tgcgttgtct gatgatttat
     9721 aaaaatgagg ctttaaacat ggtacctgtg gtcgcgcttg tcgggcgccc taacgtagga
     9781 aaatccacgt tatttaaccg tctaactcgc acccgagatg cgctggttgc ggatttcccg
     9841 ggtctgactc gtgaccgtaa gtacggtcgt gcggaaattg aaggccgtga gtttatctgt
     9901 attgataccg gcgggattga tggcacagaa gacggtgtag aaacccgcat ggcggaacag
     9961 tcgctgctgg cgattgaaga agcggacgtc gtactgttta tggtggatgc gcgcgcgggc
    10021 ctgatgccgg cagatgaagc gattgccaaa catctgcgct cccgtgaaaa accgaccttc
    10081 ctggtggcaa acaaaactga cggtctggat cccgatcagg cagtggttga tttctactcg
    10141 cttggtttag gtgaaatcta cccgatcgcc gcgtctcacg gtcgtggcgt attaagtctg
    10201 ctggagcatg tgctgctgcc gtggatggaa gatctcgcac cgcaagagga agtcgacgaa
    10261 gacgctgaat actgggcgca atttgaagcg gaagagaacg gcgaagaaga agaggaagac
    10321 gacttcgacc cgcaaagtct gccgatcaaa ctggcgattg tgggtcgtcc gaacgtaggt
    10381 aagtctacac tcactaaccg tattcttgga gaagagcgcg ttgttgttta cgacatgcct
    10441 ggcacgacgc gtgacagcat ctacatccca atggaacgcg atggacgtga gtatgtgctc
    10501 attgacaccg ctggcgtacg taaacgcggc aaaatcaccg atgctgtaga gaaattctcc
    10561 gtaatcaaaa cgttgcaggc cattgaagac gccaacgtgg tgatgttagt gattgatgcg
    10621 cgcgaaggta tttccgatca ggatctctcg ctgctgggct ttattctcaa tagtgggcgc
    10681 tcacttgtca ttgtggtgaa taagtgggat ggcctgagcc aggaagtgaa agagcaggtg
    10741 aaagaaacgc tggacttccg tctgggcttt atcgattttg ctcgtgtgca ctttatctct
    10801 gccttgcacg gcagtggtgt tggtaacttg tttgaatcag tacgtgaagc gtatgacagc
    10861 tccacccgtc gtgtggggac ctctatgctg acgcgcatca tgacgatggc tgttgaagat
    10921 caccaaccgc cgctggtacg cggtcgtcgt gtgaagctga aatatgccca cgctggtggt
    10981 tataacccgc cgattgtggt gattcacggt aatcaggtga aagacctgcc tgattcctac
    11041 aagcgctact tgatgaacta cttccgcaaa tcgctggacg taatgggatc gccgattcgt
    11101 attcagttca aagaagggga aaacccgtat gcgaacaagc gtaacaccct gacgccaacc
    11161 cagatgcgta aacgtaagcg tctgatgaag cacatcaaga aaaataaata attatccgta
    11221 agtattttat ctgccattcc ctctatgaat gtagagggaa tggctaccgg aggaattcat
    11281 atggaactgc attgtccaaa atgtcagcat gtgcttgatc aggataacgg ccatgcgcgt
    11341 tgcccatcct gcggggaatt tattgaaatg aaagctcttt gtccagactg ccatcaaccg
    11401 ttgcaggtgt taaaagcctg cggtgcagta gattacttct gtcagcacgg gcatggcttg
    11461 atatcgaaaa aacgcgttga attcgtgctg gcttaatgaa cctttttacg cgattttttt
    11521 accggctgga tgttttttac ttcactttct atccagccgt cttccagacg tgtggttagc
    11581 atttcacccg ctttaacttg cttcactttt ttcagtacat tgccgtcagt agcagtagta
    11641 acgctgtatc cacgcgccag cgttgacagt gggcttacgg cttcgaggtg cgttactgca
    11701 ttaccgaaac gttcacgcgt ggcgctaagc tgtgcgcgca ggatttctgc taaacgatat
    11761 tccagttgct gaatgcgcgt ttgcgtgcga tgaatcttcg gctgtggatt ttgctgattc
    11821 agccgctgtg ttaatcgctg ctgctgttgc ccggcacgct taagctgatt ttccagcgca
    11881 aagctcatcc gtttttgcag gcgttcaagc atggtttgct ggcgtgccag ccggagctgc
    11941 ggatgctgct gctgcaagcg atgatgaatc tgcgtaaagc gacgcgtacg gttggcgaga
    12001 taataatcca tcgccatctc cagccgttga cgggccgatt gcacctggcg cagtaactct
    12061 tgctgattac ggctcactac ttcagcggcg gcagacggcg ttggcgcacg cagatcggca
    12121 acaaaatcgg caatggtcac atccgtctca tgcccgacgg cgctgacgac cggaatgcgg
    12181 ctggcaaaaa tcgcccgcgc tacgcgttcg tcgttaaaac tccataaatc ttccagcgaa
    12241 ccgccgccgc gcccaacgat caacacgtcg cactcattgc gctgattcgc cagttcaatg
    12301 gcgcgaacaa tttgccccgg cgcgtcatcg ccctgaacgg aggtggggta gatgatcacc
    12361 ggtagagaag gatcgcgacg ttttaacaca tgcaaaatat catgtagcgc agcaccggtt
    12421 tttgaggtga tcacaccaac gcaatgcgca ggggagggaa gtggtttttt gtattgctga
    12481 tcgaacaaac cttcagcctg caacttcgct ttgagctgtt cgtacttctg ttgcagcagc
    12541 ccttcaccgg ccggctgcat actctcaacg attatctggt agtcgccgcg cggctcgtag
    12601 agcgtaatat tggcgcgaac taaaacttgt tgcccatgct gcggacggaa ggtgacccgg
    12661 cggttgctgt tgcggaacat cgcgcagcgt acctgggcgg tgtcgtcttt gagtgtaaag
    12721 taccagtgac cggaagccgg ttgtgtgaaa ttagagattt cgccgctgat ccaaacctgt
    12781 cccatctcat gctcaagcag cagacgaacc gtttgattca ggcgactaac ggtaaaaatt
    12841 gcaggggatt gagaaggtaa catgtgagcg agatcaaatt ctaaatcagc aggttattca
    12901 gtcgatagta acccgccctt cggggatagc aagcattttt tgcaaaaagg ggtagatgca
    12961 atcggttacg ctctgtataa tgccgcggca atatttatta accactctgg tcgagatatt
    13021 gcccatgcta cgtatcgcta aagaagctct gacgtttgac gacgttctcc tcgttcctgc
    13081 tcattctacc gttctgccga atactgctga cctcagcacc cagctgacga aaactattcg
    13141 tctgaatatc cctatgcttt ccgcagcaat ggataccgta acggaagcgc gcctggctat
    13201 tgctctggct caggaaggcg gtatcggctt tatccacaaa aacatgtcca ttgaacgcca
    13261 ggcagaagaa gttcgccgtg tgaaaaaaca cgaatctggt gtggtgactg atccgcagac
    13321 tgttctgcca accacgacgc tgcgcgaagt gaaagaactg accgagcgta acggttttgc
    13381 gggctatccg gtcgttaccg aagaaaacga actggtgggt attatcaccg gtcgtgacgt
    13441 gcgttttgtt accgacctga accagccggt tagcgtttac atgacgccga aagagcgtct
    13501 ggtcaccgtg cgtgaaggtg aagcccgtga agtggtgctg gcaaaaatgc acgaaaaacg
    13561 cgttgaaaaa gcgctggtgg ttgatgacga attccacctg atcggcatga tcaccgtgaa
    13621 agacttccag aaagcggaac gtaaaccgaa cgcctgtaaa gacgagcaag gccgtctgcg
    13681 tgttggtgct gcagttggcg caggtgcggg taacgaagag cgtgttgacg cgctggttgc
    13741 cgctggcgtt gacgttctgc tgatcgactc ctcccacggt cactcagaag gcgttttgca
    13801 gcgtatccgt gaaacccgtg ctaaatatcc ggatctgcaa atcatcggcg gcaacgtggc
    13861 aacagctgca ggtgcacgcg ctttggcaga agctggttgc agcgcggtta aagtcggtat
    13921 cggccctggt tctatctgta cgactcgtat cgttactggc gtaggtgttc cgcagatcac
    13981 tgccgttgct gacgcagttg aagccctgga aggcaccggt attccggtta tcgcagatgg
    14041 tggtattcgc ttctccggcg acatcgccaa agctatcgcc gctggcgcaa gcgcggtgat
    14101 ggtaggttcc atgctggcgg gtactgaaga atctccgggt gaaatcgaac tctaccaggg
    14161 ccgttcttat aaatcttacc gtggtatggg ttccctgggc gcgatgtcca aaggttcctc
    14221 tgaccgttat ttccagagcg ataacgctgc cgacaaactg gtgccggaag gtatcgaagg
    14281 tcgcgtagct tataaaggtc gcctgaaaga gatcattcac cagcagatgg gcggcctgcg
    14341 ctcctgtatg ggtctgaccg gctgtggtac tatcgacgaa ctgcgtacta aagcggagtt
    14401 tgtacgtatc agcggtgcgg gcattcagga aagccacgtt cacgacgtga ccattactaa
    14461 agagtccccg aactaccgtc tgggctcctg attctcttcg cccgacttca tgtcgggcga
    14521 tttatattat ctgtttcact tgcctcggaa taagcgtcaa tgacggaaaa cattcataag
    14581 catcgcatcc tcattctgga cttcggttct cagtacactc aactggttgc gcgccgcgtg
    14641 cgtgagctgg gtgtttactg cgaactgtgg gcgtgggatg tgacagaagc acaaattcgt
    14701 gacttcaatc caagcggcat tattctttcc ggcggcccgg aaagtactac tgaagaaaac
    14761 agtccgcgtg cgccgcagta tgtctttgaa gcaggcgtac cgatattcgg cgtttgctat
    14821 ggcatgcaga ccatggcaat gcagttgggc ggtcacgttg aagcctctaa cgaacgtgaa
    14881 tttggctacg cgcaggtaga agtcgtaaac gacagcgcac tggttcgcgg tatcgaagat
    14941 gcgctgaccg cagacggtaa accgctgctc gatgtctgga tgagccacgg cgataaagtt
    15001 accgctatcc cgtccgactt cgtcaccgta gccagcaccg aaagctgccc gtttgccatt
    15061 atggctaacg aagaaaaacg cttctatggc gtacagttcc acccggaagt gactcacacc
    15121 cgccagggta tgcgcatgct ggagcgtttt gtgcgtgata tctgccagtg tgaagccctg
    15181 tggacgccag cgaaaattat cgacgatgct gtagctcgca tccgcgagca ggtaggcgac
    15241 gataaagtca tcctcggcct ctctggtggt gtggattcct ccgtaaccgc aatgctgctg
    15301 caccgtgcta tcggtaaaaa cctgacttgc gtattcgtcg acaacggcct gctgcgtctc
    15361 aacgaagcag agcaggttct ggatatgttt ggcgatcact ttggtcttaa cattgttcac
    15421 gtaccggcag aagatcgctt cctgtcagcg ctggctggcg aaaacgatcc ggaagcaaaa
    15481 cgtaaaatca tcgggcgcgt tttcgttgaa gtattcgatg aagaagcgca gaaactggaa
    15541 gacgtgaagt ggctggcgca gggcaccatc taccctgacg ttatcgaatc tgcggcttct
    15601 gcaaccggta aagcacacgt catcaaatct caccacaacg tgggcggcct gccgaaagag
    15661 atgaagatgg gcctggttga accgctgaaa gagctgttca aagacgaagt gcgtaagatt
    15721 ggtctggagc tgggcctgcc gtacgacatg ctgtaccgtc atccgttccc gggaccaggc
    15781 cttggcgttc gtgttctggg tgaagtgaag aaagagtact gtgacctgct gcgccgtgct
    15841 gacgccatct tcattgaaga actgcgtaaa gcggacctgt acgacaaagt cagccaggcg
    15901 ttcaccgtct tcctgccagt acgttccgtt ggcgtaatgg gcgatggtcg taagtatgac
    15961 tgggttgtct ctctgcgtgc tgtcgaaacc atcgacttta tgaccgcaca ctgggcgcat
    16021 ctgccgtacg atttcctcgg tcgcgtttcc aaccgcatta tcaatgaagt gaacggtatt
    16081 tcccgcgtgg tgtatgacat cagcggcaag ccgccagcta ccattgagtg ggaatgattt
    16141 tgatccctgc actatgaatg aacaaaaccc tccgttacta cagagggttt tttatcttca
    16201 agaatcatag gcttgaagtt actaacatcg attaattaaa ccagctgtcc gatttgttct
    16261 cttctgcttt gcccacgctt ttcatcagat cgcgaccgcc ttcagtcata tttctgtttg
    16321 cgtcagcttc agattgcacc acatcggttt gcgcagcttt gtgcttcagt tcctgatcga
    16381 taaattcgtt ttcgcgctta acgcgggctt cttctttcgc cagcgccagt ttttgtttct
    16441 gaatctctaa gctgcgtagc tcatcttcat aactttgatc gcgttttttg tccgcagtgg
    16501 cttcggcgtc cagtttatcc tgacgagctt tcttatttgc cgctgccgtt gccgctcttt
    16561 tgttcgcggc ggcctgggca tttgcgcgac gttgcttctc ttgctggatt tccctgttgc
    16621 gctccgcgac ccattcgtca tgctgccttt gctcttcatt tttaccttgc tgttccgctt
    16681 ctg
//
LOCUS       DAJTYF010000083        16663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTYF010000083 DAJTYF010000000
VERSION     DAJTYF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.083"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..942
                     /locus_tag="OO590_002810"
     CDS             <1..942
                     /locus_tag="OO590_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HCR6638113.1"
                     /translation="TGLGTITAANTVTVRSRVNGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
     gene            942..4091
                     /gene="mdtB"
                     /locus_tag="OO590_002811"
     CDS             942..4091
                     /gene="mdtB"
                     /locus_tag="OO590_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR6638114.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVALSTLLLSVLLWVFI
                     PKGFFPVQDNGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTN
                     PSLNSARLQINLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRT
                     QYQFTLQATSLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLG
                     ISMADVDNALYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGV
                     VPLSSIAKIEQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVD
                     ITTQFQGSTLAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGA
                     LLALMIAGSELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLL
                     RFRPILMTTLAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLL
                     FDRLVLWTKSRFARHEEEA"
     gene            4092..7157
                     /gene="mdtC"
                     /locus_tag="OO590_002812"
     CDS             4092..7157
                     /gene="mdtC"
                     /locus_tag="OO590_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6638115.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLRITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            7158..8573
                     /locus_tag="OO590_002813"
     CDS             7158..8573
                     /locus_tag="OO590_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6638116.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            8570..9973
                     /gene="baeS"
                     /locus_tag="OO590_002814"
     CDS             8570..9973
                     /gene="baeS"
                     /locus_tag="OO590_002814"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6638117.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            9970..10692
                     /gene="baeR"
                     /locus_tag="OO590_002815"
     CDS             9970..10692
                     /gene="baeR"
                     /locus_tag="OO590_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6638118.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            10883..11215
                     /locus_tag="OO590_002816"
     CDS             10883..11215
                     /locus_tag="OO590_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6638119.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            11362..12723
                     /gene="yegQ"
                     /locus_tag="OO590_002817"
     CDS             11362..12723
                     /gene="yegQ"
                     /locus_tag="OO590_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6638120.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(13065..13382)
                     /locus_tag="OO590_002818"
     CDS             complement(13065..13382)
                     /locus_tag="OO590_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638121.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            13788..14687
                     /gene="yegS"
                     /locus_tag="OO590_002819"
     CDS             13788..14687
                     /gene="yegS"
                     /locus_tag="OO590_002819"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6638122.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(14769..15542)
                     /locus_tag="OO590_002820"
     CDS             complement(14769..15542)
                     /locus_tag="OO590_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6638123.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(15648..16655)
                     /gene="gatD"
                     /locus_tag="OO590_002821"
     CDS             complement(15648..16655)
                     /gene="gatD"
                     /locus_tag="OO590_002821"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6638124.1"
                     /translation="MRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPRIFKNGAHYYPI
                     TLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQCAKYDFIGSRR
                     DGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCENKNVIIIGAGT
                     IGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRF
                     NQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSW
                     MNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIARNAMPGKVLLI
                     P"
BASE COUNT         3844 a         4463 c         4380 g         3976 t
ORIGIN      
        1 accgggcttg gcaccattac cgccgctaat actgttacgg tgcgcagccg cgtgaacggt
       61 caactgatgg cgttacattt ccaggaaggc cagcaggtca aagcaggcga tttactggca
      121 gaaattgacc ccagccagtt caaagttgca ttagcacaaa cccagggcca actggcaaaa
      181 gataaagcca cgcttgctaa cgcccgccgt gatctggcgc gttatcagca actggcaaaa
      241 accaatctcg tctcccgtca ggagctggat gcccaacagg cgctggtcag tgaaaccgaa
      301 ggcaccatta aggctgatga agcaagcgtc gccagcgcgc agttgcaact cgactggagc
      361 cgcatcaccg caccagtcga tggtcgcgtt ggtctcaagc aagttgatgt tggtaaccaa
      421 atctccagtg gtgataccac cggaattgtg gtgatcaccc agacgcatcc tatcgattta
      481 gtctttaccc tgccggaaag cgatatcgct actgtagtgc aggcgcaaaa agccggaaaa
      541 ccgctgatgg tagaagcctg ggatcgcacc aattcgaaga aattaagtga aggcacgctg
      601 ttaagcctcg ataaccaaat cgatgccaca accggtacga ttaaagtgaa agcacgcttt
      661 aataatcagg atgatgcgct gtttcccaat cagtttgtta acgcgcgcat gttagtcgac
      721 accgaacaaa acgccgtagt gatcccaaca gccgccctgc aaatgggcaa tgaaggccat
      781 tttgtctggg tgctgaatag cgaaaacaag gtcagcaaac atctggtgac gccgggcatt
      841 caggacagtc agaaagtgga agtggtggaa gcccagagcg ccaccactcc ggaagagaaa
      901 gccaccagcc gcgaatacgc gaaaaaagga gcacgctcct gatgcaggtg ttacccccga
      961 gcagcacagg cggcccgtcg cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga
     1021 tggtggcgat cttactcgcc gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc
     1081 cggaagtgga ctatccgacc attcaggtgg tcacgctcta cccaggtgcc agcccggatg
     1141 tcatgacctc tgccgttacc gcgccgctcg aacgccagtt cgggcagatg tctggcctga
     1201 aacagatgtc gtcgcaaagt tccggcggtg cgtcagttat cactttgcag ttccagctaa
     1261 cattaccgct caatgtcgcc gagcaggaag tgcaggccgc gattaacgct gcgaccaact
     1321 tgttgccgag cgatctgcct aacccgccgg tttacagcaa agtgaacccg gcagatccgc
     1381 cgatcatgac gctcgccgtc acctcaaccg ccatgccgat gacgcaagtg gaagatatgg
     1441 tggaaacccg cgtcgcgcag aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt
     1501 ccggcggtca gcgtccggct gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg
     1561 gcctgaccag cgaaaccgtg cgcaccgcca ttaccggcgc taacgttaac tcggcaaaag
     1621 gtagcctcga cggcccttcc cgtgcggtca cgctttccgc gaacgaccag atgcaatccg
     1681 ccgaagagta tcgccagcta atcatcgcct accagaacgg cgcgccaatt cgtctgggcg
     1741 atgtagcaac tgtagagcaa ggtgcagaaa acagctggct cggcgcgtgg gcgaacaaag
     1801 aacaggccat tgtgatgaat gttcagcgcc agcccggtgc taacattatc tccaccgccg
     1861 acagcattcg gcagatgctg ccacagctca ctgagagtct gccgaaatcg gtgaaggtga
     1921 cagtgctttc cgatcgcacc accaatatcc gcgcatccgt cgatgatact cagtttgaat
     1981 tgatgatggc tatcgcgctg gtagtcatga ttatctacct gtttttgcgc aatattccgg
     2041 cgaccatcat tcccggtgtt gctgtaccgc tgtcgttaat cggcactttc gcggttatgg
     2101 tgtttctcga tttttcaatc aataacctga cactgatggc gttaactatc gccaccggat
     2161 tcgtggtcga tgacgccatc gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg
     2221 aaaaaccgtt ggcggcggcg ctcaagggcg caggtgaaat cggctttacc attatctcgc
     2281 tgaccttctc actgattgcg gtgttgatcc cactgctgtt tatgggcgat atcgtcgggc
     2341 gactgttccg cgaatttgct attaccctgg cggtagcgat tttgatctca gcggtggtgt
     2401 cgctgaccct gacaccgatg atgtgcgcgc ggatgctcag ccaggagtcg ttgcgtaaac
     2461 agaaccgctt ctcccgtgcc tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc
     2521 gtggactggc gaaagtgctg aatcatccgt ggctgacctt aagcgtggca ctcagcacgc
     2581 tgctgcttag cgtggcactc agcacgctgc tgcttagcgt gctgctgtgg gtgttcattc
     2641 cgaaaggttt cttcccggta caggacaacg gcattattca gggcactttg caggcaccgc
     2701 aatccagctc ctttgccaat atggcccagc gacaacgcca ggtcgcggac gtgattttgc
     2761 aggatccggc agtgcaaagc ctgacctcat ttgttggcgt tgatggcact aacccgtcgc
     2821 tgaatagtgc acgtttacaa atcaacctca aaccgttgga tgaacgtgat gatcgggtgc
     2881 aaaaagtcat cgcccgtctg caaacggcgg tagataaagt gccgggcgtc gatctcttcc
     2941 tgcaaccaac gcaggacctg accatcgata ctcaggtcag ccgcacccag taccagttta
     3001 ccttgcaggc cacgtcactg gatgcgctca gtacctgggt gccacagttg atggaaaaac
     3061 tccagcaact gccacagctt tctgatgtct ccagcgactg gcaggacaaa gggctggtgg
     3121 cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg gatcagcatg gcggacgtcg
     3181 ataacgccct gtacaacgcg tttggtcagc gactgatttc cactatttat actcaggcta
     3241 accagtatcg cgtggtgctg gagcacaata ccgaaaatac cccaggcctc gcggcgctgg
     3301 ataccattcg cctgaccagc agcgacggtg gcgtggtgcc gttaagctca attgccaaaa
     3361 ttgagcagcg ttttgcgccg ctttccatca accatctgga tcagttcccg gtaacaacca
     3421 tctcctttaa cgtgccggat aactattcgc tgggcgatgc ggtgcaggcg attatggaca
     3481 ccgaaaagac gctgaatctg ccggtggata tcaccacgca attccaaggc agcaccctcg
     3541 ccttccagtc ggcgctgggc agcactgtct ggctgattgt cgcggcggtg gtggcgatgt
     3601 atatcgtgct cggcattctg tacgagagct ttattcaccc gatcaccatt ctctcgacgc
     3661 tacccaccgc aggggtcggc gcgctgctgg cattaatgat tgccggtagc gaactggatg
     3721 tgattgcgat tatcggcatt attttgctga tcggtattgt gaagaagaac gccatcatga
     3781 tgatcgactt cgcgctggct gctgagcgcg agcaaggcat gtcgccgcgc gatgcaatct
     3841 accaggcttg tctgttgcgt tttcgtccga tcctgatgac caccctggcg gctctgcttg
     3901 gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga actgcgtcgt ccgttaggta
     3961 tcggcatggt cggcggtctg attgtcagcc aggtgctgac gctgttcacc acgccggtga
     4021 tttatctgct gttcgaccgc ctggtattgt ggaccaaaag tcgctttgct cgccatgaag
     4081 aggaggcgta agtgaagttt tttgccctct tcatttaccg cccggtggcg acgattttac
     4141 tgtcggtcgc cattaccctg tgcggcatac tgggtttccg tatgctgccg gtcgccccgc
     4201 tgccgcaggt cgattttccg gtgattatgg tcagcgcctc gctgcccggt gcgtcgccgg
     4261 agacaatggc gtcttccgtt gccacgccgc tggagcgctc acttgggcgc attgccgggg
     4321 ttagtgaaat gacctccagc agttcgctcg gcagcacgcg cattattttg cagtttgatt
     4381 ttgaccggga tatcaacggc gcagcgcgtg atgtgcaggc ggcgatcaac gctgcacaaa
     4441 gtttgctgcc cagcgggatg cccagccgcc cgacctatcg caaagcgaac ccgtcggatg
     4501 cgccaattat gatcctcacg ctgacatccg atacttattc gcagggtgaa ctgtacgatt
     4561 tcgcctcgac gcagctggct ccgacgattt cgcaaatcga cggtgtcggt gatgtcgatg
     4621 tcggtggcag ctcgctgccc gccgtgcgcg tcgggctgaa cccgcaggcg ctgtttaatc
     4681 agggcgtgtc gctggacgac gtacgcactg ccatcagcaa tgccaacgtg cgtaaaccgc
     4741 agggggcgct ggaagatggc actcaccgct ggcagatcca gaccaacgat gaactgaaaa
     4801 ccgccgctga atatcagccg ctgattattc actacaacaa cggcggcgcg gttcgtctgg
     4861 gcgatgtggc gacggtgact gactcagtgc aggatgtgcg caacgccggg atgaccaacg
     4921 ccaaaccggc tattttactg atgatccgca aactgccgga agccaatatt atccagacgg
     4981 tagacagcat ccgggcaaaa ttaccagagt tgcaggagac cattccggcg gcgattgatc
     5041 tgcaaattgc ccaggatcgc tcccccacca ttcgcgcctc gctggaagaa gtcgagcaaa
     5101 cgctgattat ctcggtggcg ctggtgattc tggtggtctt tttattcctg cgctcgggtc
     5161 gcgccactat tattcccgcc gttgcggtgc cggtttcgct gattggtacg tttgcggcga
     5221 tgtacctgtg cggattcagt ctcaataatc tttcgctgat ggcgctcacc attgctactg
     5281 gtttcgtggt ggatgacgcc attgtggtgc tggaaaacat tgcccgtcat ctggaagcgg
     5341 ggatgaaacc actacaggcc gcgctgcaag gtactcgcga agtcggcttt acggtgctgt
     5401 cgatgagtct gtcactggtg gcggtgttcc tgccgctgct gttgatgggc ggattgccgg
     5461 gccgactgtt acgcgagttt gccgtgacgc tttctgtcgc cattggtatt tcgttgctgg
     5521 tttccctgac gctaacgcca atgatgtgcg gctggatgct aaaagccagc aagccgcgtg
     5581 agcaaaagcg actgcgtggt tttggccgca tgttggtagc cctgcaacaa ggctacggca
     5641 agtcgctgaa atgggtgctc aatcataccc gtctggtggg cgtggtgctg cttggcacca
     5701 ttgcgctgaa tatctcgatc ccgaaaacct tcttcccgga gcaggacacc ggcgtactga
     5761 tgggtgggat acaggcggat cagagtattt cgtttcaggc gatgcgcggc aaattgcagg
     5821 atttcatgaa aattatccgt gacgatccgg cagtggataa tgtcaccggc tttaccggcg
     5881 gttcacgagt taacagtggg atgatgttta tcaccctcaa gccacgcgac gaacgcagcg
     5941 aaacggcgca gcaaattatc gaccgtctgc gcgtgaagct ggcgaaagaa ccgggggcga
     6001 atctgttcct gatggcggta caagatattc gcgttggtgg gcgtcagtcg aacgccagct
     6061 accagtacac gttgttatcc gacgacctgg cggcactgcg cgaatgggag ccgaaaatcc
     6121 gcaaaaaact ggcgacgttg ccggaactgg cggacgtgaa ctccgatcag caggataacg
     6181 gcgcggagat gaatctggtt tacgaccgcg acaccatggc acggctggga atcgacgtac
     6241 aggccgccaa cagtctgtta aataacgcct tcggtcagcg gcaaatctcg accatttacc
     6301 agccgatgaa ccagtataaa gtggtgatgg aagtggatcc gcgctatacc caggacatca
     6361 gtgcgctgga aaaaatgttc gttatcaata acgaaggcaa agcgatcccg ctgtcgtatt
     6421 tcgctaaatg gcaaccggcg aatgccccac tatcggtgaa tcatcaggga ttatcggcgg
     6481 cctcgaccat ctcgttcaac ctgccgaccg gaaaatcgct ctcggacgcc agtgcggcga
     6541 tcgatcgcgc aatgacccag cttggtgtgc cttcgacggt gcgcggcagt tttgccggca
     6601 cggcgcaggt tttccaggag acgatgaact cgcaggtgat cctgattatc gccgccatcg
     6661 ccacggtgta tatcgtgctg ggtatccttt acgagagtta cgtacatccg ctgacgattc
     6721 tctccaccct gccctcggcg ggcgttggag cgctgttggc gctggagctg ttcaatgccc
     6781 cgttcagcct aatcgccctg atagggatca tgctattaat cggcatcgtg aagaaaaacg
     6841 ccattatgat ggtcgatttt gcgcttgaag cccaacggca cggtaacctg acgccgcagg
     6901 aagctatttt ccaggcctgt ctgctgcgtt ttcgcccgat tatgatgacc accctggcgg
     6961 cgctgtttgg cgcgctgccg ctggtattgt cgggcggtga cggctcggag ctgcggcaac
     7021 ccctgaggat caccattgtc ggcggtctgg tgatgagcca gctcctgacg ctgtatacca
     7081 cgccggtggt gtatctcttt ttcgaccgtc tgcggctgcg tttttcgcgt aaacctaaac
     7141 aaacggtaac cgagtaaatg acagatcttc ccgacagcac ccgctggcaa ttatggattg
     7201 tggctttcgg cttctttatg cagtcgctgg acaccaccat cgtaaacacc gcccttccct
     7261 caatggcgca aagcctcggg gaaagtccgt tgcatatgca catggtcatt gtctcttatg
     7321 tgctgaccgt ggcggtgatg ctgcccgcca gcggctggct ggcggacaaa gtcggcgtgc
     7381 gcaatatttt ctttaccgcc atcgtgctgt ttactctcgg ttcactgttt tgcgcgcttt
     7441 ccggcacgct gaacgaactg ttgctggcac gcgcgttaca gggcgttggc ggtgcgatga
     7501 tggtgccggt cggcaggttg acggtgatga aaatcgtacc gcgcgagcaa tatatggcgg
     7561 cgatgacctt tgtcacgtta cccggtcagg tcggtccgct gctcggtccg gcgctcggcg
     7621 gtctgctggt agaatacgcg tcgtggcact ggatcttttt gatcaacatt ccggtgggga
     7681 ttatcggtgc gatcgccaca ttgatgttaa tgccgaacta caccatgcag acgcggcgct
     7741 ttgatctttc cggcttttta ttgctggcgg ttggcatggc ggtattaacc ctggcgctgg
     7801 acggcagtaa aggtacaggt ttatcgccgc tggcgattgc tggcctggtc gcagttggcg
     7861 tggtggcact ggtgctttat ctgctgcacg ccagaaataa caaccgcgcc ctgttcagtc
     7921 tgaaactgtt ccgcactcgt accttttcgc tgggcctggc ggggagcttt gccggacgta
     7981 ttggcagtgg catgttgccc tttatgacac cggttttcct gcaaattggc ctcggtttct
     8041 cgccgtttca tgccggactg atgatgatcc cgatggtgct tggcagcatg ggaatgaagc
     8101 gaattgtggt acaggtggtg aatcgctttg gttatcgtcg ggtactggta gcgaccacgc
     8161 tgggtctgtc gctggtcacc ctgttgttta tgactaccgc tctgttgggc tggtactacg
     8221 ttttgccgtt cgtcctgttt ttacaaggga tggtcaactc gacccgtttc tcctccatga
     8281 acaccctgac gctgaaagat ctcccggaca atctggcgag cagcggaaac agcctgctgt
     8341 cgatgattat gcaattgtcg atgagtatcg gcgtcaccat cgccgggctg ttgctggggc
     8401 tttttggttc gcagcatgtc agcgtcgaca gcggcaccac acaaaccgtc tttatgtaca
     8461 cctggcttag catggcgttt atcatcgccc ttccggcgtt catctttgcc agagtgccga
     8521 acgatacgca tcaaaatgta gctatttcgc ggcgaaaaag gagcgcgcaa tgaagttctg
     8581 gcgacccggt attaccggca aactgtttct ggcgattttc gccacctgca ttgtcttgct
     8641 gatcagtatg cactgggcgg tgcgtatcag ttttgagcgt ggctttattg attacatcaa
     8701 acacggcaat gaacagcgat tacagctgtt aagtgatgcg cttggcgagc agtatgcgca
     8761 gcatggtaac tggcgcttcc tgcgcaacaa cgatcgcttt gtctttcaga tcctgcgttc
     8821 atttgaacac gataattcgg aagataaacc cggcccgggt atgccgccgc acggctggcg
     8881 tacccagttc tgggtggtcg atcaaaacaa caaagtactg gttggtccgc gagcgccgat
     8941 tccacctgac ggtacacggc gacccattct ggtcaacggt gcggaagtgg gcgcggtgat
     9001 tgcctcccct gttgagcggc tgacccgcaa tactgatatc aatttcgata aacaacagcg
     9061 gcaaaccagc tggctgatcg tcgccctggc aacgttactc gcggcgctcg ccacctttct
     9121 gctggcgcgc ggtttgctgg caccggtaaa acgacttatc gatggcacgc acaaactggc
     9181 ggcgggcgat ttcactaccc gcgtaacgcc caccagtgaa gatgaactgg gcaaactggc
     9241 gcaagacttc aaccagctcg ccagcacgct ggagaaaaac cagcaaatgc ggcgcgattt
     9301 tatggccgat atttctcacg aactgcgtac gccattagcg gtgctgcgcg gtgaactgga
     9361 agccattcag gatggcgtgc gcaaattcac gccggagacg gtggcgtctt tacaggcgga
     9421 ggtcggtaca ctgaccaaac tggttgacga tcttcatcag ttgtcgatgt ctgatgaagg
     9481 cgctctcgcc tatcaaaaag caccggtaga tttgatccca ctgctggaag tggcgggcgg
     9541 cgcatttcgc gaacgattcg ccagccgtgg cctgaaactg caattttccc tgccagacag
     9601 tatcaccgta tttggcgatc gcgaccgttt aatgcagtta ttcaataact tactggaaaa
     9661 cagcctgcgc tacactgaca gcggcggcag cctgaaaatc tctgccgagc agcacgacaa
     9721 aacggtgcgc ctgacctttg ccgacagtgc gccaggtgtc agtgacgatc agctacaaaa
     9781 attgtttgaa cgtttttatc gcaccgaagg ttcccgcaac cgcgccagcg gcggttccgg
     9841 gctggggctg gcgatttgcc tgaacattgt tgaagcccat aatggccgca ttattgccgc
     9901 ccattcgcct tttggcgggg taagcattac agtagagtta ccgctggaac gggatttaca
     9961 gagagaagta tgaccgagtt accaatcgac gaaaacacac cgcgtatttt gatcgtggaa
    10021 gatgaaccga agctggggca gttgctcatt gattatctgc gtgctgcgag ctatgcgccg
    10081 acgcttatca gccacggcga tcaggtactg gcgtatgtgc gccagacacc accggatctg
    10141 atcctgttgg atctgatgct ccctggcacc gatggcctga cattatgccg ggaaattcgt
    10201 cgtttttccg atgttccgat cgtgatggtg acggcaaaaa tcgaagagat cgaccgcctg
    10261 ctggggctgg agattggcgc cgatgactac atctgcaagc cgtacagccc acgggaagtg
    10321 gtagcgcgtg tcaaaaccat tttgcgccgt tgtaaaccgc aacgcgaact tcagcaacag
    10381 gatgccgaaa gcccgttgat tatcgacgaa ggtcgttttc aggcttcatg gcgcggtaaa
    10441 atgcttgacc tgacgcctgc ggaatttcgt ctgcttaaaa cgctctctca cgaaccagga
    10501 aaagtgttct cccgcgagca attgctcaat catctttatg acgactaccg cgtagtaacc
    10561 gaccgcacca ttgacagcca cattaaaaat ctgcgccgca agctggaatc tctcgacgcc
    10621 gaacagtcat ttatccgcgc cgtttacggc gttggttacc gctgggaagc cgacgcctgc
    10681 cgcatcgttt agttttagcg acattacttt gttagccgga gatggcgttc agacccgatc
    10741 cggcaacatt attccacgca tggtcagcaa actgacatta tctctccgcc ctggcaggca
    10801 tcagaaaatc gctcatactt taatcagtaa acagcacctt tagatgctgt tttcgataag
    10861 caatttcaat caaggagtca ttatggctgg ttggtttgaa ctcagtaaga gcagtgataa
    10921 tcagttccgg tttgtgctaa aagcgggcaa tggtgagact atcctcacca gcgagctgta
    10981 tacctcaaaa gcctctgcgg aaaagggcat cgcgtcggtg cgtagcaaca gcccgcaaga
    11041 agagcgctat gagaaaaaaa cggcaagcaa cggcaaattc tatttcaatc tgaaagccgc
    11101 taatcatcaa attatcggct ccagccagat gtacgccacc gcgcaatctc gtgaaaccgg
    11161 aattgcctcc gttaaagcca atggcacaag ccagacggtg aaagacaata cgtaattacg
    11221 atgccgggcg tgggttgttg gcgtccggcg ctttaccaga tcccccctac agcgctacaa
    11281 tgcccgccct taaagtgggg gcctccccta accgcttcat caggtgaagc ggatctgacc
    11341 tgtcatcaga acgagagaat tatgtttaaa ccggaactcc tttccccggc gggaacgctg
    11401 aaaaatatgc gttacgcttt cgcttatggc gcagatgctg tttatgcggg ccagccgcgt
    11461 tactccctac gtgtgcgcaa caacgaattc aaccacgaaa atcttcagct cggcatcaat
    11521 gaagcccacg cgctggggaa aaagttttat gtcgtggtca acattgcacc acacaacgcc
    11581 aagctgaaaa cctttatccg tgacctgaaa ccggtggtgg aaatggggcc ggatgcgctg
    11641 attatgtccg atccagggct gattatgctg gtgcgtgagc acttcccaga aatgccgatc
    11701 cacctctcgg tgcaggctaa cgccgtgaac tgggcgacgg tgaaattctg gcagcaaatg
    11761 ggactgaccc gcgtgatcct ctctcgcgag ctgtcgctgg aagagattga agagatccat
    11821 aatcaggtgc cggatatgga gatcgagatc ttcgttcacg gcgcactgtg catggcctac
    11881 tccggtcgct gcctgctctc tggctatatc aacaagcgcg acccgaacca gggcacctgc
    11941 accaacgcct gccgctggga gtacaacgtc caggaaggga aagaagatga cgttggcaac
    12001 atcgtacaca agtacgagcc gattccggtg caaaatgttg agccgacgct gggtatcggc
    12061 gcgccaaccg acaaagtgtt tatgatcgaa gaagcccagc gtccgggcga gtatatgacc
    12121 gcgtttgaag atgagcacgg cacttacatc atgaactcga aagatctgcg cgccatcgcc
    12181 catgtagaac gcctgaccaa aatgggcgtg cattcgctga aaatcgaagg tcgtaccaaa
    12241 tctttctact attgcgcacg caccgcacag gtttaccgca aagctatcga tgacgccgct
    12301 gcgggaaaac cgttcgatac cagcctgctg gaaactctgg aaggtctggc gcatcgtggc
    12361 tataccgaag gtttcctgcg tcgtcatact cacgacgatt atcagaacta cgaatacggt
    12421 tattcagttt ctgaccgcca gcagtttgtt ggtgagttta ccggtgagcg caagggggac
    12481 ctcgcagcgg tagcggtgaa aaataaattc tccgttggcg acagtcttga gctgatgacg
    12541 ccgcaaggca acattaactt tacccttgag cacatggaaa acgccaaagg cgaagctatg
    12601 ccggtcgcac caggcgatgg ttatactgtg tggctcccgg tcccgcagga tcttgagcta
    12661 aattacgcgc tgctaatgcg taatttctcc ggggaaacca cgcgtaaccc ccacggtaag
    12721 tgattaattt cgattatttt tcccggatgg aaaattctta gaaaccgatc acatacagct
    12781 gcatttatta aggttatcat ccgtttcgct gaaaaacata atccataaaa tgctagctgt
    12841 accaggaacc acctccttag cctgtgtaat ctcccttaca cgggcttatt ttttgtacat
    12901 aagctgtaaa gaaggattga tccctgataa ccgtcttcca ttgaccacat cgatagaatc
    12961 ccccttcata gcacaatgcc tttcacgtaa cggtatcgtg ctcgcccagg ttccggctac
    13021 gcacagccag aacgcgcatg tttgacgctt accaaaaaat attctcactc tccacatttg
    13081 aatgtcagac gagcgacgcc atgtaatcct gcaccttctg tcttcaggtc aactatctgc
    13141 atttttttgc cctgagtaac acagaaatgg gctgcatcat tttttactat attttctgca
    13201 ccagatattc tgcccctggc taaagaagct tcggcttcgg tgtagtattg gttatcgagt
    13261 ttacgctgaa tattactttt atatgcaaga ccaaatttac cgatacttgt ttcatcatta
    13321 tgcacagcac aaccagatat aagaaaaata ctaattaatg atatagcagc tatctttttc
    13381 atctcacctt cccccattaa ataccaacga cactctctag tgtttaaata taataatggc
    13441 atgattatta taattgaata ggattataat aaatgttctg tacaacattt cctacataag
    13501 taggaattac ggacattgag gcccttcagg gtaactccat gggggcttta atatattata
    13561 ttgaagatgc cactgtttag ttgaatatta ggtatatgct cttttttgaa atttataggt
    13621 ggcagccgtt agtattcgct gtccccattg caagctcctg gtggtaacca ctgaatcctc
    13681 catacttgaa ctgacttttt atcctccgac tttcatcctg ttctgactcc accttttgtt
    13741 ttctgctcta cactatctac agaccaatca taaaggcaca tacgatcatg gcagaatttc
    13801 ccgccagctt actgattctt aatggcaaaa gtactgacaa tctacccttg cgcgaagcaa
    13861 ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg ggtcacctgg gagaaaggtg
    13921 atgccgcacg atttgtagag gaggcccgga agttgggcgt cgcaacggtg attgccggtg
    13981 gtggcgatgg caccattaat gaagtttcta cggcgttgat tcagtgtgag ggggatgaca
    14041 tacccgcgct gggaattttg ccattaggaa ccgccaatga ttttgccacc agtgtaggga
    14101 ttcctgaggc actggataag gcgctgaaac tggcaattgc cggtaatgcc attgcgatag
    14161 atatggcgca ggtcaacaaa caaacctgtt ttattaatat ggcgacaggc ggatttggga
    14221 cgcgtattac cacagaaacg ccggaaaaat taaaagccgc gctgggtggc gtctcttaca
    14281 tcattcatgg cttaatgcgc atggatactc tgcaaccgga ccgttgtgaa atccgcggtg
    14341 aaaactttca ctggcaaggt gacgccctgg tcattggtat tggtaacggg cgtcaggccg
    14401 gtggcggtca acaattgtgc ccgaacgcgt taattaacga tggcttgctg caactgcgca
    14461 tttttaccgg cgatgaaatt cttccggctc tcgtatcaac cttaaaatct gacgaagata
    14521 acccgaatat tatcgaaggc gcttcgtcgt ggtttgatat acaagcccca cacgaaatca
    14581 cttttaatct tgatggcgaa ccgttgagtg ggcaaaactt ccatattgaa atacttccgg
    14641 cggcgttgcg ttgtcgatta ccaccagatt gtccattatt gcgttaatca aactattctc
    14701 tcttgaacca gggagaatcc gtaatgccgg atgcggtgta aacaccttat ccggcataca
    14761 gaacaatact acaccgtaat taattttacc cgtgtcttct gcaatgccaa tttatccacc
    14821 tctgacacag catcatcggt aacaataatg tcaatttttt cgattggcag tacctgatta
    14881 aaaccgcgac gattaaattt cgatgaatcg agaacggcaa tcactttatt tgcggcggtg
    14941 accatcgccc cgctaatcga ataaccttca ttaaaggtcg taataccatt aaccgcatca
    15001 ataccatcag caccgacaaa cattaaatcg gcattaatat cctgcaatga acgctcggca
    15061 atggaaccat gcatcgagcg cgttttatgg cggaccgtgc caccacagac gaccagagta
    15121 atgtctttat tttcggaaag ggcaaacgct gccgggagac tgttggtaat caccgtgata
    15181 tttttagcgg tcattaatcc ttcggcgata agcattgtgg tacttccgct gtcgagaata
    15241 acggtcatcc cttcatggat cattttgact gccgcctgcg ctattctgtt ttttggcgcg
    15301 ctggcgagct gaaatcgctc tttaaacccg acttcctggg tctcggtttc actattaccg
    15361 gacattattt tcgccgcacc gccatgaaag cgcgtaacca cgcctttttg ttcgagaaag
    15421 cgcaaatcgg cacggattgt cgcttccgag gcagcaaata ctcccgccag atcctgaaca
    15481 agcacggttc cctgttcatt cactaattgg atgatcttat tccttcgctc gaatgaattc
    15541 atcgtcattt acccatattc aattgtggct agtgtaaacg aagggggacc attggtgaat
    15601 gatctgtttt gcacaatacc gggccagcat cacgctggcc cgcggtttca tggaatgagc
    15661 aacactttgc ccggcatggc attacgagcg atgtcacgca ccgcctgggt gaagctttca
    15721 aagcttccac ggtgagcgat taatggatcc aggcttaact tacgttctgt cagcaaccgg
    15781 ctcgccgttt cccactcctg ccccggccaa gggctggaat agttcatcca actgccgata
    15841 accgtcagct ctttacgcaa tattttgcca aacgttgccg atgttaaatg cagatcctga
    15901 tgcaacgtgc ccaccagcgc cagttgggca tgaggaccgg caatctctac cgccagttcg
    15961 acagtttgcg gtacgccagc cgtctcgagg ataagctgat taaagcgcag ttcgcgtaaa
    16021 acgctctgca tttgcggcgc gctcatttcg ctactgttaa atgtttgcat cgcaccgaaa
    16081 gattttgcca gtgccagttt ttctgagcta atgtcgatcg ccgtcacact ctttgctccc
    16141 agcgcgacag cgcactgaat agccagcagg ccaatggttc cggcaccaat aataataacg
    16201 tttttattct cacaaccttg cgctaaatga aaagcatgca ggccaacggt aatcggctca
    16261 ataaaagccc catcctcaat aggcatatcc gtgggtagag caaagacatt ttttcgctta
    16321 acgacaatat attcagcaaa tccaccatca cgccgcgagc caataaaatc atattttgcg
    16381 cactgggaat aaaacccttt caaacactct ggacaagtaa aacagggtaa taacggcaca
    16441 caggcaaccg catcgccagg gtgtaaatca tcaacaccgg atcccaccgc atcaatatag
    16501 ccgctaaatt catggcctaa cgttattgga taataatgtg caccattttt aaatatcctg
    16561 ggtaaatcgg aaccacataa gcccgagctg gcaattttta cccgcacctc atcctgatgt
    16621 ttaatttcag gaatgacgct ttttgcaacg cgcacgatac cat
//
LOCUS       DAJTYF010000084        16520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTYF010000084 DAJTYF010000000
VERSION     DAJTYF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.084"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..1800
                     /gene="ldtD"
                     /locus_tag="OO590_002822"
     CDS             <1..1800
                     /gene="ldtD"
                     /locus_tag="OO590_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6638125.1"
                     /translation="CLAVTFAPLFNAQADEPEVIPGDSPVAVSEQGEALPQAQATAIM
                     AGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARDMQPMWENRDAVKAFQQ
                     QLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGYLHFIANIPIKGTRWLY
                     SSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYAAMHESLLALLSDTKPW
                     PQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGDDTPTDAVVSPSAVTVE
                     TAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGLGADGAIGPATRDWLNV
                     TPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNGNQVLDSRVIVGRPDRK
                     TPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESHGYTVMRGWNSREAIDP
                     WQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYLHDTPNHNLFKRDTRAL
                     SSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNIRQSIPVNLYYLTAFVG
                     ADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            1981..2529
                     /locus_tag="OO590_002823"
     CDS             1981..2529
                     /locus_tag="OO590_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6638126.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2556..3203
                     /gene="gloC"
                     /locus_tag="OO590_002824"
     CDS             2556..3203
                     /gene="gloC"
                     /locus_tag="OO590_002824"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6638127.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3426..4616)
                     /gene="aspC"
                     /locus_tag="OO590_002825"
     CDS             complement(3426..4616)
                     /gene="aspC"
                     /locus_tag="OO590_002825"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6638128.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(4801..5889)
                     /gene="ompF"
                     /locus_tag="OO590_002826"
     CDS             complement(4801..5889)
                     /gene="ompF"
                     /locus_tag="OO590_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6638129.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6491..7891)
                     /gene="asnS"
                     /locus_tag="OO590_002827"
     CDS             complement(6491..7891)
                     /gene="asnS"
                     /locus_tag="OO590_002827"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6638130.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8061..9263)
                     /gene="pncB"
                     /locus_tag="OO590_002828"
     CDS             complement(8061..9263)
                     /gene="pncB"
                     /locus_tag="OO590_002828"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6638131.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9529..12144
                     /gene="pepN"
                     /locus_tag="OO590_002829"
     CDS             9529..12144
                     /gene="pepN"
                     /locus_tag="OO590_002829"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6638132.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12351..13118)
                     /gene="ssuB"
                     /locus_tag="OO590_002830"
     CDS             complement(12351..13118)
                     /gene="ssuB"
                     /locus_tag="OO590_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6638133.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13115..13762)
                     /locus_tag="OO590_002831"
     CDS             complement(13115..13762)
                     /locus_tag="OO590_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6638134.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNI
                     DRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGI
                     GYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLERLWLSWNPAYHLKEATI"
     gene            complement(13774..14919)
                     /gene="ssuD"
                     /locus_tag="OO590_002832"
     CDS             complement(13774..14919)
                     /gene="ssuD"
                     /locus_tag="OO590_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR6638135.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(14916..15875)
                     /gene="ssuA"
                     /locus_tag="OO590_002833"
     CDS             complement(14916..15875)
                     /gene="ssuA"
                     /locus_tag="OO590_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6638136.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTIGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(15868..16443)
                     /gene="ssuE"
                     /locus_tag="OO590_002834"
     CDS             complement(15868..16443)
                     /gene="ssuE"
                     /locus_tag="OO590_002834"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6638137.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4122 a         4283 c         4231 g         3884 t
ORIGIN      
        1 tgcctggccg taacattcgc tccactgttc aatgcgcagg ccgatgagcc tgaagtaatc
       61 cctggcgaca gcccggtggc tgtcagtgaa cagggcgagg cactgccgca ggcgcaagcc
      121 acggcaataa tggcggggat ccagccattg cctgaaggtg cggcagaaaa agcccgcacg
      181 caaatcgaat ctcaattacc cgcaggttac aagccggttt atcttaacca gcttcaactg
      241 ttgtatgccg cacgcgatat gcaacccatg tgggaaaacc gtgatgctgt taaagccttc
      301 cagcaacagc tggcagaggt ggcgattgct ggtttccagc cgcagtttaa taaatgggta
      361 gagttactga ccgatcctgg tgttaacggg atggcacgtg acgtggtgct ctctgatgcg
      421 atgatgggct atctccattt cattgcaaat attccgatca aaggcactcg ctggctatat
      481 agcagtaaac cttatgcgct ttcaacgccg ccgctttcgg tgattaacca atggcagcta
      541 gcgctggata aaggtcaatt gcctacgttt gttgcaggac tggcaccgca gcatccgcaa
      601 tatgcggcga tgcatgaatc gttactggcc ttactcagtg acaccaaacc gtggccccaa
      661 ctgaccggca aagcaacgtt gcgcccaggg cagtggagta acgacgtacc ggcgttgcgc
      721 gaaatattgc aacgcacagg catgttggac ggggggccga aaattactct acctggcgat
      781 gacacgccaa ctgacgcggt agtcagccca tccgctgtta ctgttgaaac agcagaaact
      841 aagccgatgg acaagcaaac gacgtctcgt agtaaacctg cgcctgccgt tcgtgccgcc
      901 tacgataatg aactggtgga agccgttaaa cgttttcagg catggcaagg attgggagca
      961 gatggtgcta ttggcccggc aacgcgtgac tggttaaacg taacgcccgc ccagcgtgct
     1021 ggcgtgttgg cactcaacat ccagcgattg cgcttgctgc caacagagct ttctaccggg
     1081 atcatggtta acattccagc ctattcgctg ctctactatc agaacggcaa tcaggtgctg
     1141 gattcgcgag tcattgtcgg tcgccccgat cgcaaaacgc cgatgatgag cagtgcactt
     1201 aacaacgtag tggtaaaccc gccgtggaac gtaccaccaa ctctggcacg caaagatatt
     1261 ctgccgaaag tgcgcaacga tccgggatat ctcgaaagcc atggctatac ggtgatgcgc
     1321 ggctggaaca gcagagaagc gattgaccca tggcaggttg actggtctac aatcacggcc
     1381 tcgaatttac cgtttcgctt ccagcaggct ccaggcccac ggaactcgct ggggcgctat
     1441 aaattcaata tgccgagttc agaggccatt tatttgcatg acacgccgaa ccacaatctg
     1501 ttcaagcgtg atacacgcgc attgagctca ggctgtgtac gagtgaataa agcttccgat
     1561 ctggcgaata tgctgttgca ggatgcagga tggaatgaca aacgtatttc tgatgcgctg
     1621 aagcagggtg atacacgtta cgtcaatatt cggcagtcga ttccggtgaa tctctactac
     1681 ctgacggcct ttgttggtgc agatgatcgt acccagtatc gtacagatat ttacaattat
     1741 gatctgcctg cgcgatccag ctcgcaaatc gtatcgaaag cggaacaatt aatcaggtaa
     1801 atgaagtagt tcggggaaat caattgtcgt aataattagc atgattgggg gcgattctct
     1861 gcagcccccg tcactgctgg ggttgagtca tcttgacgtc tgctttacgg gcggttaagg
     1921 tgcctcttgt gcgccagaag tgcatataaa cgataacatt gacctgtaga cttgattatc
     1981 atggacaaat tcgacgctaa tcgccgcaaa ttgctggcgc ttggtggcgt tgcactcggt
     2041 gccgccatcc tgccgacccc tgcgtttgca acactctcta ccccacgccc gcgcattttg
     2101 acactcaata atcttcatac cggagagtca atcaaagcgg agtttttcga tggcagaggc
     2161 tatattcagg aagaattggc aaaacttaac cattttttcc gcgattaccg cgcgaacaaa
     2221 ataaagtcca tcgacccagg attattcgac cagttgtatc gcctgcaagg gttgttaggc
     2281 acgcgcaaac cggtgcaact catttccggt tatcgttcca ttgataccaa caatgaacta
     2341 cgcgcccgca gccgtggagt agcgaagaaa agctatcaca ctaaaggcca ggcgatggat
     2401 ttccatattg aaggtatcgc gttaagcaat attcgcaaag ccgcgttatc tatgcgcgca
     2461 ggtggtgtag gatattaccc acgtagtaac tttgtgcata ttgataccgg gccagcacgg
     2521 cactggtagt aatcgcttaa cgaaacaggg gcagtatgaa ctatcgtatt attccggtca
     2581 ccgcattctc ccagaactgt tcattgatct ggtgtgaaca aacccgcctg gccgcactgg
     2641 tcgatcctgg cggcgatgcg gaaaaaatca aacaggaagt tgatgacagc ggcctgacac
     2701 tgatgcagat cctgctgacg catggtcatc tggaccacgt tggcgcagcg gcggaactgg
     2761 cgcaacatta cggcgtgccg gtttttggcc cggaaaaaga agatgagttc tggctgcaag
     2821 gcttgcctgc gcaaagtcgt atgtttggtc tggaagagtg ccagtcgctg acgccagatc
     2881 gttggctgaa cgaaggcgat accatcagca tagggaatgt gactttacag gtgttacatt
     2941 gccctgggca tacgccgggt catgtcgtgt tttttgatga tcgggcaaag ctgctgattt
     3001 ctggcgatgt tattttcaaa ggcggagtag ggcgcagtga cttcccgcgt ggcgatcata
     3061 atcaactgat ttcttcaatc aaagataaat tgctgccact gggggatgac gtgacattta
     3121 ttccgggtca cggaccatta tctacacttg gttatgagcg cctgcacaat cccttcctgc
     3181 aagacgaaat gcccgtctgg taaaggcgca taaaaaagtc cacttttaat gcgggcctgg
     3241 atttctggca aagtgcgctt tgtttatgcc ggatgcggca cgagcgcctt atccggccta
     3301 caaaatcgtg caaattcaaa atattgcagg ggacgcgtag gcctgataag cgtagcgcat
     3361 caggcaatgt tgcgtttgtc atcagtctca gcccgctttt cagcgggctt cattgttttt
     3421 aacgcttaca gcactgccac aatcgattcg cacagcggag ccatgttatc tggtgtcatc
     3481 ccggccacat ttacgcgacc agaagcaaca gcatatacgc caaactcttc gcgcagacgc
     3541 agcacttgtt cttttgtcag accactgaag gagaacatgc cgttctgttt gatgataaag
     3601 ctgaagtcgc ggtttgcacc tttttcctgc agcgtattga cgaacaactg acgcatacgc
     3661 tgaatacgct ggcgcatatc agtcagctct tgttcccaaa tcgcacgtaa tgcatcgttg
     3721 ctcaggatgg tggcaacaac agaagcgccg tgtgctggag ggttagagta gttagcgcga
     3781 atcgccgctt tcatttggct gaatgcgcga tcaacgattt cactgtcggc agcaaccaga
     3841 gtacaagcgc caacacgctc gttgtacagg ccaaagtttt tagagtagga actggcaaca
     3901 atcagctctt tatgcatagc cgagaaagcg cgcagtcctt cagcatcttc ttccagacca
     3961 cgggcaaaac cctggtaagc gaagtcaaac agcggtaacc agcctttctc aacggagagt
     4021 tgtgccagtg tctgccattg ttccagcgta gggtcgatac cggttgggtt atggcagcag
     4081 ccatggaaca gcactacgtc gccagcctga gcttcgttca gactgttaat cagtgcatcg
     4141 aagtcaaggg tgtgattttc cgcatcataa taagcgtatt cacgaacttc cagacctgca
     4201 gagttaaaga cgctcttatg gttcggccag cttgggttgc tcacccacac acgcttaacg
     4261 ctggtatttt ttgccaggaa gtcggcagcc acgcgtagtg cgccagtgcc acccggagtc
     4321 tgtgccgtgc gagcacgttt gtcattgatc agggcgctac ctttaccaaa cagcagttcc
     4381 tgagtgcagc gaccaaattc agggatgccg tcaatgccga ggtaattttt ggtggtttca
     4441 ttttcgagca gatactgttc agcctttttc acgctggtca gtaccggggt tttgcccgtc
     4501 tcatctttat agacaccaat cccgaggtta attttgccgg gacgttcatc ggcacgaaac
     4561 agatcggcca ggcccagaat cgggtcggca ggagcggcgg taatgttctc aaacatgacg
     4621 aggttccatt atggttacag aagggaagtc cgctatcagg gtaacgggag atttacaaaa
     4681 ttccaattat tactgatgaa aacgcaggct gtttttgcaa gacgtgagat tgctctggaa
     4741 ggcataaaaa aaacaggacc aacgtcctgt tttttcggca tttaacaaag aggtgtgcta
     4801 ttagaactgg taaacgatac ccacagcaac ggtgtcgtct gaacctacgc ccagtttgtt
     4861 gtcagaatcg atctggttga tgatgtagtc aacataggtg gacatgtttt tgttgaagta
     4921 gtaggttgcg cccacttcaa agtagttcac cagatcaaca tcaccgatac cttctacgtc
     4981 tttcgcttta gatttggtgt aagcgatgga cggacgcaga ccgaaatcga actggtattg
     5041 cgcaactaac agaacgtctt gcgttttgtt ggcgaagccg ctggtgtttg taaatttatt
     5101 agtgatcggc gtagcgttac gggtttcacc gtagttcgct gccaggtaga tgttgttcgc
     5161 gtcgtacttc agaccagtag cccactgttc agctttttta ccgttgccaa gaggttgagc
     5221 ttcttgcagg ttggtacggt cagctgcacc ataagcacca acgataccaa agccttcgta
     5281 ttcgtagctg atagaaccgc caacaccgtc gccgttagaa cggcgtgcag tgtcacgctc
     5341 gtttttaccc aggtactgaa cagcgaagtt caggccatca accagaccaa agaagttgga
     5401 gttacgatag gtagcaacgc cgccaacacg accaacgaag aagtcatcgc tgtatgcagt
     5461 atcaccacca aattctggca gcatatcggt gtaacccagt gcatcataaa ccacaccgta
     5521 gttacggccg taatcgaaag aaccaacgtc agcgtattta agacccgcga atgccagacg
     5581 cgttttgtta ccagtttgag cgtcagcgcc ttcagagttg ttaccctgga agttatattc
     5641 ccactgacca taaccggtca gatcggaatt gatttgagtt tcccctttaa aaccaagacg
     5701 ggcataggtc atgtcgccat tgccaccgta actgttttca ccgttaccct tggaaaaata
     5761 atgcagacca acagctttac cgtacagatc tactttgttg ccatctttgt tatagatttc
     5821 tgcagcgttt gcagtacctg ctactaacag agcagggacg atcactgcca gaatattgcg
     5881 cttcatcatt atttattacc ctcatggttt tttttatgac acctgccact gccgtcaata
     5941 agttctgtca ataaaaattt acggaactat tgatgagagt ttggtgtctt tatgtgtctg
     6001 caggcatctt tccattcaaa ctaacgtttc gctaccgtga aagtgctaca aagataaaga
     6061 tttagtttca aaaagaaaaa atatgtaacc aaaagtaaaa attaaggagc tttgtgaaca
     6121 ccgtcatatt tccatagaaa cgtgatgata tttacagcaa tttttatcta tttatatgat
     6181 ttacttatat ttaaattaac taaacggaaa ttttgtttct gatggaaact ttatcgacct
     6241 ggcacaaaat cctctttcag acatccagaa tgccaaaaaa tagtatgaaa attgtgctat
     6301 cagctaagaa aaaactaatc cgcaataaga atgcgggtta ttttttgtgc gtaaatgttg
     6361 caatgtaata ttccgtaaca ggatgatcgt tatggcgggg gtgcaaatag attgcttgac
     6421 ttaacaaaca gataacttga cagaaaggat aaaaaagagc cagcaggcgc tggctctttg
     6481 aggaagaaaa tcagaagctg gcgttgcgcg gagtacgtgg gaacggaatc acatcacgca
     6541 cgttttgcac gccagttacg taagcaatca ggcgttcaaa accaagaccg aaacctgaat
     6601 gcggaacagt accgtagcga cgcagatcgc gataccacca gtaatcttct ttattcaggc
     6661 ccatttccag catacgctcg tccagaacgt ccagacgttc ttcacgctgg gagccaccaa
     6721 tgatctcacc gatgcccgga gccagaacgt ccatagccgc aacggtttta ccgtcttcgt
     6781 tgaggcgcat atagaacgct ttaatatctt tcggatagtt tttaaccact accggtgctt
     6841 taaagtgttc ttccgccaga taacgctcat gctcagaaga gagatcgacg ccccagtaaa
     6901 ccgggttttc aaacttcctg ccgcagtttt cgagaatggt cactgcgtcg gtgtaatcca
     6961 cctgtgcgaa atcggcttca atgaagcgtt ccagacgtga aacggcatct ttatctacgc
     7021 gttcagcgaa gaatttcatg tcgtctgcgc gttcttcgag aaccgctttg aagacatatt
     7081 tcagcatggc ttcagccaga cccgcgatat cgttcaggtt agcaaacgcc acttccggct
     7141 ccagcatcca gaattccgcc aggtgacggc tggtgttgga gttttcagca cggaaagtcg
     7201 ggccgaaggt ataaattttg gataatgcgc aagcgtaggt ttcgccgttc aactggccag
     7261 atacggtcag gaaagactct ttaccaaaga agtctttgtc gaaatccact ttgccctgat
     7321 cgttacgcgg caggttttcc agatccagcg tagaaacgcg gaacatttcg cctgcacctt
     7381 cggtatcaga tgcggtaatc agtggcgttg aaacccagaa gaatccctgc tcgttaaaga
     7441 agcgatgcag cgcctgcgcc agcgtatggc gaacgcgcgc gacggcacca atcaggtttg
     7501 tgcgcggacg caggtgagcg acttcacgca gatactcaat gctgtggcgt tttgccgcca
     7561 tcgggtaagt gtctggatct tcaacccaac cagcaacttc aaccttgctg gcctgaattt
     7621 caaattgttg cccctggccc ggcgatgcca cgactttacc cgtcacaatg accgagcagc
     7681 cggtggtcag acgcaggacg tcttcattgt aattgggcag agaattattg atgacagcct
     7741 gtacaggatc aaagcaggaa ccgtcataaa cggcgaggaa ggagatgcca gcttttgaat
     7801 ctcggcgggt acgtacccat ccgcgtacgg tgacttcgct gtcaacggct acgcggccct
     7861 ggagtacgtc ggctacaggc acaacgctca taatattctc tctgttaata gtcggaaaaa
     7921 ataaacactt gtccacccgt aaatgggggg ggatacctat gttacctggc atctgcaatc
     7981 agacaagcag aattcgcaaa tgcagcgaaa gatttcggaa ttaaaggtaa aaaagggagc
     8041 cgattagctc cctgatgata ttaactggct tttttaatat gcggaaggtc gaacgctttg
     8101 cgcagcgccc gaacaaacgc tttatcatgg cagatagttt tgccagggct gtcagaaagt
     8161 ttcgccaccg gtttaccgtt acactctacc aacttaatca caatattcag gggttttacc
     8221 tgggggatat cgcaggtcag gcgagtccca ataccaaaac ttaattgcac gcgggaagag
     8281 aagtggcggt atagctcaac cgctttgcgt aaatccagat tgtcagagaa aaccagcgtt
     8341 ttactctgtg gatcaattcc cagcttttca taatgtgcaa tggctttttc accccattca
     8401 accgggtcgc cagagtcatg acgcaggccc tgataccgac tagcgaactc gacaccgaaa
     8461 tcacgcagga aagcatccat agtgatgcag tcggttaatg caatgccaag ttggtcggga
     8521 tactcttcca gccaggcagc aagtgcagct cgctggctgt tggctagatc cgggctgatt
     8581 tgctgatgtg cctggaacca ttcgtgtgcc tgtgttccca tcggcgtgag ggaaagccga
     8641 cgcgccagat cgtagttgct ggtgcccacg aaccaggatt cctgttgcag acgcttaacg
     8701 atggtttctt gtacttcgcg agaaaaacgg cgacgggtgc caaaatccat cagatggaag
     8761 cgcgacatat caagaccggc ggttaacgcc gagaagtcga ctaatttgct ttccagcgtg
     8821 tcgagggctt gcgcaacgtc ggcctgcggt gagcgatagc gatgtaccat ttcactgata
     8881 accgccagca aaggaacttc ccagaggatg acttcacgcc acggaccgct taagcgaata
     8941 tccagcttgc cattatcgtt ggacacggtg acttgttccg ggttaaagcg gaactcgcgt
     9001 aaccagttaa gatagtcggc cttaaagaaa ggcagggcag agagccactg atattcatca
     9061 tcctgcaggc gcaggtgctg catcgcctga atctgttcac gaatagcatc ggcataaata
     9121 cccagcagat cgtcacctcg gcaacgaaac tccgccgcga catgcacatc gtaatagtga
     9181 tgaaacacgg cttgctgcat atgcaactta taagcatctg tatccagcaa cgagtgcaga
     9241 acaggagaag cgaattgtgt cataggtgcg cagtagcgtc ctcgtacagg agcgtttagt
     9301 acaataaaca tcttcaggaa ctgctggagt ataccttgtt tcgcaattta ttgaaccccg
     9361 atcacaccat atgccacctt tctggtcgat ggcattacgc ggtgtatgtt ataaaaatgt
     9421 agcaataaag gcgtttgtac ctgaaaagat gaagattctg catagcgcga tttacgcaac
     9481 aggaatagac tgaacaccag actctataaa agatgctaaa ggttatttat gactcaacag
     9541 ccacaagcca aataccgtca cgattatcgt gcgccggatt accagattac tgatattgac
     9601 ttgacctttg acctcgacgc gcaaaagacg gtcgttaccg cggtcagcca ggctgtccgt
     9661 catggtgcat cagatgctcc gcttcgtctc aacggcgaag acctcaaact ggtttctgtt
     9721 catattaatg atgagccgtg gaccgcctgg aaagaagaag aagagggcgc actggtcatc
     9781 agtaatttgc cggagcgttt tacgcttaag atcattaatg aaataagccc ggcggcgaat
     9841 acggcgctgg aagggcttta tcagtcaggc gatgcgcttt gcacccagtg tgaagccgaa
     9901 ggtttccgcc atattacgta ttatctcgac cgcccggacg tgctggcgcg ttttaccacc
     9961 aaaattattg ccgataaaat caaatatccc ttcctgcttt ccaatggtaa ccgcgttgcg
    10021 caaggcgaac tcgaaaacgg acgccattgg gtacagtggc aggacccgtt cccgaaaccg
    10081 tgctacctgt ttgcgctggt ggcaggcgac tttgatgtac tgcgcgatac ctttaccacg
    10141 cgttctggtc gcgaagtagc actggagctg tacgtcgatc gcggcaacct tgatcgcgcg
    10201 ccgtgggcga tgacctcgct gaaaaactcc atgaaatggg atgaagaacg ctttggcctg
    10261 gagtatgacc tcgacatcta tatgatcgtc gcggtggatt tcttcaatat gggcgcaatg
    10321 gagaataagg gtctgaatat ctttaactcc aaatatgtgc tggcccgcac cgacaccgcc
    10381 accgacaaag attacctcga tattgaacgc gttatcggcc atgaatattt ccataactgg
    10441 accggtaacc gagtgacctg ccgcgactgg ttccagctca gcctgaaaga aggtttaacc
    10501 gtcttccgcg atcaggaatt cagctctgac cttggttccc gcgcagttaa ccgcatcaat
    10561 aatgtgcgca ccatgcgcgg attgcagttt gcggaagacg ccagcccgat ggcgcaccca
    10621 atccgcccgg acatggtcat tgagatgaac aacttctaca ccctgaccgt ttacgagaag
    10681 ggcgcggaag tgatccgtat gatccacacc ctgctgggcg aagaaaactt ccagaaaggg
    10741 atgcaactct atttcgagcg tcatgatggt agcgcagcga cctgtgacga ctttgtgcag
    10801 gcgatggaag atgcgtcgaa tgttgatctc tcccatttcc gccgttggta cagccagtcc
    10861 ggtacgccgg ttgtgaccgt caaagacgac tacaatccgg aaactgagca gtacaccctg
    10921 accatcagcc agcgcacgcc agccacgccg gatcaggcag aaaaacagcc gctgcatatt
    10981 ccgtttgcca tcgaattgta tgacaacgaa ggcaaagtga tcccgttgca gaaaggcggt
    11041 catccggtga attccgtgct gaacgtcacc caggcggaac agacctttgt ttttgataat
    11101 gtctacttcc agccggtgcc tgcgctgctg tgcgaattct ctgcgccagt gaaactggaa
    11161 tataagtgga gcgatcagca actgaccttc ctgatgcgtc atgcgcgtaa tgatttctcc
    11221 cgttgggatg cggcgcaaag tttgctggca acctacatca agctgaacgt cgcccgtcat
    11281 cagcaagggc agccgctgtc tctgccggta catgtggctg acgctttccg cgcggtgctg
    11341 ctcgatgaga agattgatcc ggcgctggcg gcagaaatcc tgacgctgcc ttctgtcaat
    11401 gaaatggctg aactgttcga tatcatagac ccgattgcta ttgccgaagt acgcgaagca
    11461 ctcactcgta ctctggcgac tgaactggcg gatgagctgc tggctattta caacgcgaat
    11521 taccagagcg agtaccgtgt tgagcatgaa gatattgcaa aacgcactct gcgtaatgcc
    11581 tgcctgcgct tccttgcttt tggtgaaacg catctggctg acgtgctggt gagcaagcaa
    11641 taccatgaag caaacaatat gaccgatgcg ctggcagcgc tttctgcggc ggttgctgca
    11701 cagctgcctt gccgtgacgc gctgatgcag gagtacgacg ataagtggca tcaggacggt
    11761 ctggtgatgg ataaatggtt tatcctgcaa gccaccagcc cggcggcgaa tgtgctggag
    11821 acggtgcgtg gtctgttgca gcatcgctca tttaccatga gcaacccgaa ccgtattcgt
    11881 tcgttgattg gtgcgtttgc gggcagcaac ccggcagcgt tccatgccga agatggcagc
    11941 ggttaccagt tcctggtgga aatgcttacc gacctcaaca gccgtaaccc gcaggtagct
    12001 tcacgtctga ttgaaccgct gattcgcctg aaacgttatg atgccaaacg tcaggagaaa
    12061 atgcgcgcgg cgctggaaca gttgaaaggg ctggaaaatc tctctggcga tctttacgag
    12121 aagataacta aagcactggc ttgatacttt tgagccaaaa tgcctgatgc gacgcttgac
    12181 gcgtcttatc aggcctacaa ctccgttgaa attgctggtt ttgtaggctg gataaggcgt
    12241 tcacgccgca tccggcagcc gtgcccaact gcctgatgcg atgcttgacg tgtcttatca
    12301 ggcctacaag tccgttgaaa atgctggttt tgtgcgccac atccggcata ttaaccatga
    12361 cgccgaatca gttgttcaga atgcccacgc cgcattaccc gttgcaatac ttccgcttcc
    12421 agctccgcta atcgcaccga ccctaatcga cgcggacggg ggatatccac tgtcaaatcc
    12481 agactaattt ttccctcttc aattaacaac acccggtcag ccattgctac cgcttcgctc
    12541 acatcatgcg tcaccaacag tacggtaaag ccgtgctgct gccaaagtga cacaatcaaa
    12601 tcctgcatct cgagtcgcgt taaggcgtcc agtgccccga gcggttcatc aagcaacaat
    12661 aaacccggtc gatgaatcaa cgctcttgcc agcgccacac gctgtttctg cccgccagaa
    12721 agtgcggcag gccattcccc ggcgcgattc tccagtccta ccgcagccaa cgcccgacgc
    12781 gcggcatcgc gccactgacc tttaaggcct aacccaacgt tatcaatcac cgatttccac
    12841 ggcagcagac gcgcatcctg aaacatcatt cgcgtatcat cctgaatttc agccagcggt
    12901 gtggtgcccg ctaacacatc gcctgcggtt ggcgtttcca gaccggccag caggcgcagc
    12961 agggtacttt taccaccacc gctgcggccc accaccgcca caaactgacc tgccggaata
    13021 tgtaaatcca gttggttcag gacgatattt tccgcgtaat gtttgcttac tgcattgagc
    13081 aacaatggtg tgccctggtt cagacgagca gtattcatat cgtggcctcc ttcaaatgat
    13141 aagccgggtt ccagctcaac cagaggcgct ctaacagctg cgcgctgacg tcagcaagtt
    13201 tgccgagcag ggcgtaaaga ataatagcga ctaccaccac gtctgtttgc aaaaactctc
    13261 gcgcattcat cgccagataa ccaatgccgg aattggcaga aatggtttcg gcaacaatca
    13321 gcgtcagcca catcaggccc aacgcaaaac gcacgccgac cataattgag ggcagggcat
    13381 cgggcaggat cacatggata aacagcggta tgccggataa tccatagcta cgcgccatct
    13441 ccaccagccc gcgatcgata ttacggatgc catgccaggt gttgatataa atggggaaca
    13501 gcgtaccgag cgccaccaga aagattttcg cggattcatc aatgccaaac cacaaaatca
    13561 ccagcgggat cagcgccaga tgctgccaca gttcgccgct ggcggagagc gtccagaacg
    13621 ccgtcaccac tccttccggt gaaggcaaaa tacgcgtcga cagccagcca accgaggagg
    13681 ccagttgcca caccgccacg atgcccaccg gtaaaaacca gggggcaacg cgcaataacc
    13741 acttcttcac tggcgttgcc atcatcgtgt tccttagctt tgcgcgactt tacgggggat
    13801 aaaatcattc gccaccgctt cgccttgcgg attcagcggc tgcggctggg gaatttccgg
    13861 aatggcgaca tccagatgtg ggaacagcaa ctcgccaacc cgatacgctt cttccagatg
    13921 cgggtagccc gaaagcacaa agctgtcgat gccaagcgcg gcgtattcgt tgattcgcgc
    13981 ggcgaccgta ggaccatcgc ccaccagcgc cgttccggca ccgccgcgca ctaagccaac
    14041 gcccgcccat aaattggggc tgatctccag attgtcgcgc ttgccgttat gtagcgccgc
    14101 cattcgctgt tgtcctacgg aatccgtccg ggcgaatgcg gcttgtgctt tggcgatagt
    14161 ctcatcatca agatgcgaga ttaaccgctc ggcggcctgc cacgcttcgt cgttagtttc
    14221 acgaacaatc acatgcagac gaataccgaa acgaattttg cgcccatacg cggcagcttt
    14281 cgcccgcact tgttcgattt tctctttaac aagttccggc ggttcgcccc aggtgaggta
    14341 gagatcaact tgttctgccg ccagctcctg ggcgacatct gacgatccgc caaagtaaag
    14401 cggaggataa ggctgttgaa tcggcgggaa gagcagtttt gctccgcgca catgaatatg
    14461 tttaccgttg aaattgacgg tttcccccag caataagcgc cgccagacct gggtaaattc
    14521 cgccgaggct tcgtagtgct cgctatgatc aaggaacacg ccgtcgcctg ccagctcttg
    14581 tggatcgctg cctgtgacca gattaaacaa cgcacgacca tttgagagac ggtcaagcgt
    14641 ggcggcctgg cgggcggcaa cagtaggtga ggttacgctg ggacgcaggg cgacaagaaa
    14701 cttcagccgc tgcgtcaccg ggatcatcga cgctgcaacc agccacgcat cttcacacga
    14761 tcgccccgtt gggatcagca caccggtata gcccagacga tccgccgctt gcgcaatttg
    14821 ttgcagataa ccgtgatcaa ccgggcgtga accttcttcc gttcccagat agtgcccgtc
    14881 accgtgggtc ggtaaaaacc agaacatatt cagactcata attgttttcc ttccagttga
    14941 gtgggctgcc agatgcgctg gcgaatatcg acttttttcg gcaccagacg gttttcataa
    15001 aacagatctg ccgtttgctg ctgtaaggcg gcaacctcgg cgttaaccgg tttgatggtg
    15061 gtaggagggc gatgatctaa gtaagaggca atcaccggtg ccggtaagcc aatcgttttt
    15121 gccagtaaag cgatgctttg ttcgcgctgg ctgcgggtta acgcatcggc ctcactaaag
    15181 gttgccagta cgccctgaat aaaagcgccg tttttttctg catagggacg agctgccaga
    15241 taaaacgatc cagtttgatt gagatcggtg ccgtctttca gcacccgcac gccgccctgt
    15301 aataacgcag cggagtagta gggatcccag atagcccagg cgtcaacgtt accttgctgg
    15361 aacgcggcgc gggcaccggc aggcgtcagg taagtgggct ggatatcggt gaatttaagt
    15421 ccggcctgac gcagtgcacg cagtaaaagg ttgtgtgaac tggaaccttt ctgaaaggca
    15481 actttgtgac ctttaagatc ggctacggtt ttgatcgggc tgttttctgc caccagaatc
    15541 acttcggctt tgggcttcgg tggctcgacg cccacatata ccaaatcagc cccggcagcc
    15601 tgggcaaaga ttggcggaat atccccggta ctgccgagat caatactgcc aacgtttaac
    15661 gcttccaaca tttgcggacc cgcggggaac tccacccagg agatttttga ttgcggatag
    15721 cgtttttcca gtaactggtg gctttttgcc agcaccatac caatactgcc tttctgatag
    15781 cctatacgta acgcttcagg cgaggattct gcagcaaccg caaaagtaga gacgctaagc
    15841 aatcccgcca gcgccagttt aatgatgtta cgcatgggca ttacctcgca gagacagaag
    15901 gtcaggaacc tgaacatcgc ggcggtgcaa tgcctgccag aaagtttcta gcgcggtatc
    15961 aagatggggt tgcagatttg gcgtgaactg gggtctgtga tggtaatcaa ttaattgtga
    16021 gtcatcggca aacacgccgt gcaggatctc ctgagctttc agtgcgctta aaactggttt
    16081 aagggcataa tcgaccgcca gcagatgggc cacggtaccg cccgtcgcca gcggtagcac
    16141 cactttgcct tgcaaagcgc gctctggcag caggtcgagc agggttttca gcgcgccgga
    16201 ataggcggct ttatacacag gcgtggcgac aatcagccca tcagcctgtt gcagctgttc
    16261 ggtgaaggtc ttgagtgccg gactatcgaa acgagcataa agcagatctt ccggggcgaa
    16321 gttttgcaga ttccagtgat aaacctctac atctaggcca tttagttttt cccgcgcata
    16381 ttccagcaag gagctggagc gagaaggaaa gcgaggacta cccgccaggg tgatgacacg
    16441 catactctct ccttataacc aattgttctt ttttttgtta acattgataa caattcggtc
    16501 agtctgtcgg agagacaaga
//
LOCUS       DAJTYF010000085        16322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTYF010000085 DAJTYF010000000
VERSION     DAJTYF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.085"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="OO590_002835"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="OO590_002835"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6638138.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYEVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="OO590_002836"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="OO590_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6638139.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="OO590_002837"
     CDS             2337..2552
                     /locus_tag="OO590_002837"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638140.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="OO590_002838"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="OO590_002838"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6638141.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /gene="galP"
                     /locus_tag="OO590_002839"
     CDS             4447..5841
                     /gene="galP"
                     /locus_tag="OO590_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6638142.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWRTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="OO590_002840"
     CDS             5918..6415
                     /locus_tag="OO590_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6638143.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="OO590_002841"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="OO590_002841"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6638144.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="OO590_002842"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="OO590_002842"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6638145.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="OO590_002843"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="OO590_002843"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6638146.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9100..9663
                     /locus_tag="OO590_002844"
     CDS             9100..9663
                     /locus_tag="OO590_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6638147.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="OO590_002845"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="OO590_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6638148.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="OO590_002846"
     CDS             complement(10255..11235)
                     /locus_tag="OO590_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6638149.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="OO590_002847"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="OO590_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6638150.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="OO590_002848"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="OO590_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6638151.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="OO590_002849"
     CDS             12538..12828
                     /locus_tag="OO590_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6638152.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANNHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="OO590_002850"
     CDS             12836..13429
                     /locus_tag="OO590_002850"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6638153.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITHEPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="OO590_002851"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="OO590_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6638154.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15835
                     /locus_tag="OO590_002852"
                     /pseudo
     CDS             14872..15835
                     /locus_tag="OO590_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15880..>16322
                     /locus_tag="OO590_002853"
     CDS             15880..>16322
                     /locus_tag="OO590_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR6638155.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYA"
BASE COUNT         4039 a         4209 c         4106 g         3968 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttctg cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttctaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaggc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cctcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga atcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaaaaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggtcgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggtctgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg cctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaattga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 acttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac catattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtgga ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtctcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 agccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcactcgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccga ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gcgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgatcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata ccatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgtcatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacggc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa caatcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cacgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgttcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ccatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aagcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtgac gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattacc
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgttaga gaaattcgac aacgctgctc aataatattc
    15841 cgggccagcc tctggccctg attttccagg atgcttacaa tgatatttaa ccgcctgaaa
    15901 cttgcggtag accgcgtcat tgccgcgttt tctgtcgccg taatgcttgc tctggttgta
    15961 tgcgtagtct ggcaagtatt tagccgctac gtacttaacc agccaagtac attaaccgat
    16021 gagctggcac gctttttgat gatctgggta ggattattag gtgctgcata taccgtcggc
    16081 gcgcagcgac atctctctat tgatttattt gctctcgccc tgaataagcg caaacaatta
    16141 cttcttagta tagtcattaa cgttctcatt ttaggttttg ccggttcggt cattgtgact
    16201 ggtggcctga aattaattga taaaacactg gcaacatcac aggtatctgc cgctatgcaa
    16261 ataccgatgg gatacgtata tatcattctg ccattaagtg gagtggtaat gatgttttat
    16321 gc
//
LOCUS       DAJTYF010000086        16151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTYF010000086 DAJTYF010000000
VERSION     DAJTYF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.086"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(188..520)
                     /gene="emrE"
                     /locus_tag="OO590_002854"
     CDS             complement(188..520)
                     /gene="emrE"
                     /locus_tag="OO590_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6638156.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(865..1179)
                     /gene="fliE"
                     /locus_tag="OO590_002855"
     CDS             complement(865..1179)
                     /gene="fliE"
                     /locus_tag="OO590_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6638157.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1393..3051
                     /gene="fliF"
                     /locus_tag="OO590_002856"
                     /pseudo
     CDS             1393..3051
                     /gene="fliF"
                     /locus_tag="OO590_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            3044..4039
                     /gene="fliG"
                     /locus_tag="OO590_002857"
     CDS             3044..4039
                     /gene="fliG"
                     /locus_tag="OO590_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6638158.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4032..4718
                     /gene="fliH"
                     /locus_tag="OO590_002858"
     CDS             4032..4718
                     /gene="fliH"
                     /locus_tag="OO590_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6638159.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            4718..6091
                     /gene="fliI"
                     /locus_tag="OO590_002859"
     CDS             4718..6091
                     /gene="fliI"
                     /locus_tag="OO590_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6638160.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRSQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6110..6553
                     /gene="fliJ"
                     /locus_tag="OO590_002860"
     CDS             6110..6553
                     /gene="fliJ"
                     /locus_tag="OO590_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR6638161.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6550..7677
                     /gene="fliK"
                     /locus_tag="OO590_002861"
     CDS             6550..7677
                     /gene="fliK"
                     /locus_tag="OO590_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6638162.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSASLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGDDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7782..8246
                     /gene="fliL"
                     /locus_tag="OO590_002862"
     CDS             7782..8246
                     /gene="fliL"
                     /locus_tag="OO590_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6638163.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8251..9255
                     /gene="fliM"
                     /locus_tag="OO590_002863"
     CDS             8251..9255
                     /gene="fliM"
                     /locus_tag="OO590_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6638164.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDILPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9252..9665
                     /gene="fliN"
                     /locus_tag="OO590_002864"
     CDS             9252..9665
                     /gene="fliN"
                     /locus_tag="OO590_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6638165.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9668..10033
                     /gene="fliO"
                     /locus_tag="OO590_002865"
     CDS             9668..10033
                     /gene="fliO"
                     /locus_tag="OO590_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6638166.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10033..10770
                     /gene="fliP"
                     /locus_tag="OO590_002866"
     CDS             10033..10770
                     /gene="fliP"
                     /locus_tag="OO590_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6638167.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10780..11049
                     /gene="fliQ"
                     /locus_tag="OO590_002867"
     CDS             10780..11049
                     /gene="fliQ"
                     /locus_tag="OO590_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6638168.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11058..11762
                     /locus_tag="OO590_002868"
     CDS             11058..11762
                     /locus_tag="OO590_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HCR6638169.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFNGHLWLISLLVDTFHTLPIGSEPLNSNAFLAPTKAGSLIFLNGLMLA
                     LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFS
                     EIFNLLADIISELPLI"
     gene            12047..12670
                     /gene="rcsA"
                     /locus_tag="OO590_002869"
     CDS             12047..12670
                     /gene="rcsA"
                     /locus_tag="OO590_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6638170.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12714..12902)
                     /gene="dsrB"
                     /locus_tag="OO590_002870"
     CDS             complement(12714..12902)
                     /gene="dsrB"
                     /locus_tag="OO590_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6638171.1"
                     /translation="MKVNDRVTVKTDSGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13065..13292
                     /gene="yodD"
                     /locus_tag="OO590_002871"
     CDS             13065..13292
                     /gene="yodD"
                     /locus_tag="OO590_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6638172.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13590..14405
                     /locus_tag="OO590_002872"
                     /pseudo
     CDS             13590..14405
                     /locus_tag="OO590_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(14402..16096)
                     /gene="yedQ"
                     /locus_tag="OO590_002873"
     CDS             complement(14402..16096)
                     /gene="yedQ"
                     /locus_tag="OO590_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR6638173.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDHA"
BASE COUNT         4022 a         4156 c         4237 g         3736 t
ORIGIN      
        1 aaacgataag ttttttcata aaaacctcat tgttgtgacc tatcgttatt ttatttgatg
       61 tgccttgatc tcattatgca taaaggcaca atgatcgcgc aaatgcaatc ataattataa
      121 aggtgccccg aacattagcc ataatatgca caaatcagat tcctgttatc tcaaggagaa
      181 ttatttttta atgcggtgca cttcgtgaca acaagttaat gactaacaca ccggcacaaa
      241 tcaacatcat gcctatgata gctggcaggt ccagtcgttg accaaaaaat ccccaggaaa
      301 gcaaactaat cagaacgata ccgactcctg accagatagc ataagcaatc cctgtaggta
      361 tataagccag cgtctgagct aataaccaga atgatgcaca atagcaaata attgtgccaa
      421 cagagggcca taaccgtgta aacccctctg aaaacttcat taaggttgta ccaatgacct
      481 ctgcaagtat tgcgccacca agataaatat aagggttcat agcatattct ttcctgttca
      541 tactggagag aattgtacta cagtttgaac acaactcacc gttttcatcc tagtgtatgt
      601 gtatccaagg ggctctggcg tggcaagaga taaaggcagg aacaataatg atcagaagga
      661 accccaggct aacgaatgag ttcactcttc ctgggagcaa agcgaagaca aaacatgtta
      721 tcgcgtttac ggatcgtgga tggcagaaaa caaccaggat caggtatcca ttttaaacca
      781 gaaattgagt gagtttgccc catccatgcc ccacgctgtg ggatcggata taattaaaca
      841 agcatagtca acaggttaca caacctacac ctgcatagac atcacttcct gatacgccgc
      901 caccagctta ttacgcacct gaatccccat ttgcatagaa actgaggctt tttgcatatc
      961 ggtcatcaca tcgtttagcg ccacgccggg ttcaccgagg gtgaattttt ctgcctgcgt
     1021 gcgggcgact gtttgtgtat cgcttatgcg atcgatcgcg gcgtgcagct gcccggcaaa
     1081 actaatggtc ggttgcggca gtgattcctg cgcacgcgca ctcatcgccg tagcctgtaa
     1141 ctggctgata accccttcaa tcccctgtat cgctgacatt ttcgtctccc ggataatttc
     1201 tggtaacaaa gcctaccagt aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac
     1261 gggtttattg gcggatagaa aaaacgaaag cacaaataat gggagcgtca atttttcgag
     1321 tttgctgacc cgagagtgag tcttgttcca ctttgccaat aacgccgtcc ataatcagcc
     1381 acgaggtgcg cgatgaatgc gactgcagcc cagacaaaat ctcttgagtg gcttaatcgc
     1441 ctgcgtgcga atccgaaaat tccattgatt gttaccggtt ccgcggcagt ggcggtcatg
     1501 gtcgcactga tcctgtgggc gaaagccccc gactaccgca cattattcag caatctttcc
     1561 gatcaggatg gtggcgcaat tgtcagccaa ctgacgcaaa tgaatattcc ttaccgcttc
     1621 agcgaagcca gcggcgctat tgaggttccg gcagataaag ttcacgaact gcgcctgcgc
     1681 ctggcgcaac aaggtttgcc caaaggcggc gcggtcggtt tcgaactgct cgatcaggaa
     1741 aagtttggta tcagccagtt cagcgaacag gtgaattatc agcgggcgct ggaaggcgag
     1801 ctttctcgta ccatcgaaac tatcggcccg gtaaaagggg cgcgcgtaca tctggcaatg
     1861 ccgaaaccgt ctttattcgt ccgtgaacaa aaatcccctt ctgcatcggt gacggtaaat
     1921 ctgttaccca gccgcgcact cgatgaaggg caaattagcg ctattgtaca tctggtttcc
     1981 agcgcggttg ctggtctgcc gcctggaaac gtcacgctgg tggatcaggg cgggcatctg
     2041 ttaacccagt ccaataccag cgggcgcgat cttaatgacg ctcagttgaa atatgccagc
     2101 gatgtcgaag gccgtattca gcggcgtatt gaagcgatcc tgtcgcctat tgttggtaac
     2161 ggtaatattc acgcctaggt cacggcgcaa ctggacttcg ccagtaaaga acaaacggaa
     2221 gaacagtatc gccctaacgg tgatgaatct catgcggcgc ttcgttcacg ccagcttaat
     2281 gagagcgagc aaagcggttc cggttatccg ggcggcgtac cgggggcgtt gtcgaatcaa
     2341 ccggcacctg cgaataacgc gccaatcagc acgcctccga caaatcaaaa taaccgccag
     2401 cagcaggcga gcaccaccag caatagtggg ccgcgtagca cacagcggaa tgaaaccagt
     2461 aactacgaag tcgatcgcac cattcgtcat accaaaatga acgtgggcga tgtgcaacgt
     2521 ctgtcagtcg cggtagtggt gaattacaaa accttgccag acggtaaacc attgcctctc
     2581 agcaacgaac agatgaagca aattgaagct ctgacccgcg aggcgatggg cttttctgaa
     2641 aaacgcggtg attcgctcaa tgttgttaac tcgccgttca atagcagtga cgaaagcggc
     2701 ggagcactgc cgttttggca acagcaagtg tttatcgatc agttgctggc tgccggtcgc
     2761 tggttgctgg tactgctggt ggcgtggttg ctgtggcgga aagcggtacg cccgcagcta
     2821 acacgtcgcg ccgaggcggt gaagactgtg cagcaacagg cgcaggcccg cgaggaagtg
     2881 gaagatgcgg tggaagtccg tctgagcaaa gacgaacaac tccaacaacg gcgcgctaac
     2941 caacgactgg gggcagaagt catgagccag cgtatccgtg aaatgtctga taacgatccg
     3001 cgcgtggtgg cgctggtcat tcgccagtgg ataaataacg atcatgagta acctgacagg
     3061 caccgataaa agcgtcatcc tgctgatgac cattggcgaa gaccgggcgg cagaggtgtt
     3121 caagcacctc tcccagcgcg aagtgcaaac cctgagcgct gcaatggcga acgtcacgca
     3181 gatctccaac aagcagctaa ccgatgtgct ggcggagttt gagcaagaag ctgaacagtt
     3241 tgctgcactg aatatcaacg ccaacgatta tctgcgttcg gtattggtca aagctctggg
     3301 tgaagaacgt gccgccagcc tgctggaaga tattctcgaa acccgcgata ccgccagcgg
     3361 tattgaaacg ctcaacttta tggagccgca gagcgccgcc gatctgattc gcgatgagca
     3421 tccgcaaatt atcgccacca ttctggtgca tctgaagcgc gcccaggccg ccgatattct
     3481 ggcgttgttc gatgaacgtc tgcgccacga cgtgatgttg cgtatcgcca cctttggcgg
     3541 cgtgcagcca gccgcgctgg ccgaactgac cgaagtactg aatggcttgc tcgacggtca
     3601 gaatctcaag cgcagcaaaa tgggcggcgt gagaacggca gccgaaatta tcaacctgat
     3661 gaaaacgcag caggaagaag ccgttattac cgccgtgcgt gaattcgacg gcgagctggc
     3721 gcagaaaatc atcgacgaga tgttcctgtt cgagaatctg gtggatgtcg atgatcgcag
     3781 cattcagcgt ctgttgcagg aagtggattc cgaatcgctg ctgatcgcgc tgaaaggggc
     3841 cgagcagcca ctgcgcgaga aattcctgcg caatatgtcg cagcgtgccg ccgatattct
     3901 gcgcgacgat ctcgccaacc gtggtccggt gcgtctgtcg caggtcgaaa acgaacagaa
     3961 agcgattctg ctgattgtgc gccgtcttgc cgaaactggc gagatggtga ttggcagcgg
     4021 cgaggatacc tatgtctgat aatctgccgt ggaaaacctg gacgccggac gatctcgcgc
     4081 caccaccggc agagtttgtg ccaatggtcg agtcggaaga aaccattatt gaagaggccg
     4141 aacccagcct tgagcagcaa ctggcgcaac tgcaaatgca ggcccacgag cagggttatc
     4201 aggcggggat tgccgaaggt cgccagcaag gtcacgaaca gggctatcag gaaggactgg
     4261 cccaggggct ggaacaaggt ctggcagagg cgaaggctca acaagcgcca attcatgccc
     4321 ggatgcagca actggtcagc gaatttcaaa ctacccttga tgcgcttgat agtgtgatag
     4381 cttcgcgcct gatgcagatg gcgctggagg cagcacgtca ggtgatcggt cagacaccaa
     4441 cggtggataa ctcggcactg atcaaacaga tccaacagtt gttgcagcaa gaaccgttat
     4501 tcagcgggaa accacagctg cgcgtacacc cggatgatct gcaacgtgtg gatgatatgc
     4561 tcggcgcaac tttaagtttg catggctggc gcttgcgggg cgatcccacc ctccaccctg
     4621 gcggatgtaa agtctccgcc gatgaaggcg atctcgacgc cagtgtcgcc actcgctggc
     4681 aagaactctg ccgtctggca gcactaggag tggtgtaatg accacgcgcc tgactcgatg
     4741 gctaaccacg ctggataact ttgaagcaaa aatggcgcag ttgcctgcgg tacgtcgcta
     4801 cgggcgatta acccgcgcta ccgggctggt gctggaagcc accggattac aattgccgct
     4861 cggcgcaacc tgtgtcattg agcgccagaa cggcagcgaa acgcacgaag tagaaagcga
     4921 agtcgttggc tttaacggtc aacggctgtt tttaatgccg ctggaggaag tggaaggtgt
     4981 cctgcccggc gcgcgtgtat atgccaaaaa catttcggca gaagggctgc aaagcggcaa
     5041 gcagttgccg ctcggtccgg cgttattagg tcgtgttctg gacggtagcg gtaaaccgct
     5101 cgatggcctg cccttccccg atacgacgga aaccggtgcg ctgattaccc cgccatttaa
     5161 cccgttgcaa cgtacaccga ttgaacatgt gctggacacc ggcgtgcgcc caatcaatgc
     5221 cctgcttacc gtcggacgcg ggcagcgtat ggggctgttt gccgggtcgg gcgttggtaa
     5281 aagtgtgctg ctggggatga tggcacgtta cacccgcgcc gatgtcattg tcgtgggttt
     5341 gattggtgaa cgtgggcgcg aagtaaaaga ttttattgag aatatcctcg gtgccgaagg
     5401 acgtgcacgc tcagtggtga ttgccgctcc ggcggatgtt tctccgctcc tgcgaatgca
     5461 aggtgccgcc tatgccacgc gcattgccga agattttcgc gatcgtagtc agcatgtgtt
     5521 gctgattatg gactccctca cccgctacgc gatggcccag cgtgaaattg cgctggcgat
     5581 tggcgaacca cctgccacta aaggttatcc accgtcggtg tttgccaaat taccggcact
     5641 ggtcgagcgt gccggtaatg gcattagcgg cggcggctcg attaccgcgt tttataccgt
     5701 gctcaccgaa ggcgatgacc agcaggaccc cattgctgac tccgcgcggg caatcctcga
     5761 cggtcacatt gtgctgtctc gccgactggc ggaagccggg cactatccgg ctatcgatat
     5821 tgaagcgtcg atcagtcgcg caatgacggc gttgatcagt gagcaacatt acgcgcgagt
     5881 gcgcaccttc aaacagctgt tgtcgagttt tcagcgtaac cgcgatctgg ttagcgtcgg
     5941 cgcgtatgcc aaaggcagcg atccgatgct cgataaagcc atcgccctgt ggccgcagct
     6001 ggagggctat ttgcaacaag gcatttttga acgcgcggac tgggaagcgt ctctccaggg
     6061 gctggagcgt attttcccga cagtgtcata acccaggaga taacggcaga tggcggaaca
     6121 tggtgcgctg gcgaccctga aagatctggc agaaaaagag gtagaggatg ccgctcgcct
     6181 gctgggtgaa atgcgtcgcg gatgtcagca ggcggaagaa cagctcaaaa tgctgatcga
     6241 ttatcagaat gaatatcgca ataacctcaa tagcgatatg agtgccggga tgaccagcaa
     6301 ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta ctcagcatcg
     6361 ccagcaactt aatcagtgga cgcagaaagt tgacattgcc ctgaacagct ggcgagaaaa
     6421 gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg caatccacgg cggcactgct
     6481 tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg ccgccatgag
     6541 gaaacctgaa tgattcgctt agcgcccttg attaccgccg acgttgacac caccacattg
     6601 cctggcggca aagccagcga tgctgcacaa gattttctaa cgttgttgag cgaagcatta
     6661 gcaggcgaga caacaactga caaagcggcc ccccagttgc tggtggcaac agataagccc
     6721 acgacaaaag gcgagccgct ggtcagcgat attcttgccg atgcgcaaca agcggattta
     6781 ctgatccctg tggatgaaac acttcctgtc atcaacgacg aacaatccac atcaacaccg
     6841 ttaaccaccg ctcagacaat gacgttggct gcggtggctg acaaaaatac gacaaaagac
     6901 gaaaaagcgg atgatcttaa tgaagacgtc accgcaagtc tgagcgccct ttttgcgatg
     6961 ttgccgggtt ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt gttaccggca
     7021 gagaaaccaa cgctcttcac aaaactgact tctgcgcaac tcactacagc acagcctgat
     7081 gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc ccagagtaaa
     7141 gcggaagtca tcagcacgcc ttcgccggtg accgctgacg ccagcccgct aatcactcca
     7201 caccagacac agccactgcc caccgtcgcc gcgcctgttt tgagtgcatc gctgggttct
     7261 cacgaatggc aacaatcatt gagccagcat atttcgctgt tcacccgcca ggggcaacaa
     7321 agtgcagagt tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc cctcaaagtg
     7381 gatgataacc aggcgcagat ccagatgatt tcaccgcatc agcacgtacg cgccgccctg
     7441 gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca gttagggcaa
     7501 agcaacatta gtggcgaaag ctttagtggt cagcagcagg ccgcttccca gcaacagcaa
     7561 agccaacgca cagcaaacca tgaacctctg gcgggggatg acgacgatac gcttccggtt
     7621 cccgtctctt tacaagggcg tgtaacaggt aacagcggcg ttgatatttt cgcctaacgt
     7681 cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag acgcaggata
     7741 attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat tacgcgataa
     7801 gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc ctcgcggcct
     7861 gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgac gacaaagcgc
     7921 agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga tacctttacg gtcaatttgg
     7981 gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg actgaaagat gaagcaaccc
     8041 gctcgcggct gagtgagtat ttgccggaag tccgtagtcg cttgctgtta ctgttttcac
     8101 gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa aaacctgatt gccgagatta
     8161 aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc accgacgtgc
     8221 tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc tcaagctgaa
     8281 attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc cagtgttagc
     8341 ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt gcgcgaacgt
     8401 ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat ggggctgttc
     8461 aacctgctgc gccgtagccc ggacattacc gtcggggcca tccgcattca gccataccat
     8521 gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc taatccacct gaaaccgcta
     8581 cgcggcacag ggctggtagt gttctcgccg agtctggtgt ttatcgccgt ggataacctg
     8641 tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac ccatactgaa
     8701 cagcgcgtca tcaaccgtat gttgaagctg gcgctggaag gctatagcga cgcctggaaa
     8761 gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga atttaccaat
     8821 atcaccacct cgccgaacga cattgtggtt aacacgccgt tccatgtgga gattggcaac
     8881 ctgaccggcg aatttaatat ctgcctgcca ttcagcatga tcgagccgct acgggaattg
     8941 ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg cgataacctg
     9001 gtgcgccagg tgcagcattc acagctggaa ctggtggcca actttgccga tatctcgcta
     9061 cgcctgtcgc agattttaaa actgaaaccc ggcgacatcc tgccgataga aaaacccgat
     9121 cgcatcatcg cccatgttga cggcgtcccg gttctgacca gtcagtacgg cactctcaac
     9181 ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc tctgaacgag
     9241 gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc gcaatggacg
     9301 atctgtgggc tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc gctgccgacg
     9361 cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat atcgacctga
     9421 ttatggatat tccggtcaag ctgaccgtcg agctgggccg tacgcggatg accatcaaag
     9481 agctgttgcg tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg ggcgaaccgc
     9541 tggatattct gaacaacggt tatttaatcg cccaaggcga agtggtggtt gttgccgata
     9601 aatatggcgt gcggatcacc gatatcatta ccccgtccga gcggatgcgc cgcctgagcc
     9661 gttagtgatg aataaccatg ctactgtgca atcttccgcg ccggtttctg ctgagccact
     9721 gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg ctgcctggct
     9781 ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt aacggtctga aaattagcgc
     9841 cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag atgcacggct
     9901 ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc cttcagcacc
     9961 aacggaagag ataccgcaga ccgattttca gtcggtcatg aaaaatttgc ttaagcgtgg
    10021 cgggagatcc tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg gctgattatg
    10081 cccctcgcct tcgcgcaact gccgggtatc accagccagc cgctacctgg cggtggacaa
    10141 agctggtcgc tcccggtgca gacgctggtg ttcatcacct cgttgacgtt cattccagcg
    10201 attttactga tgatgaccag tttcacccgc atcattattg tttttggttt attgcgtaac
    10261 gcgctgggaa cgccctccgc gccacctaac caggtattgc tggggctggc actgtttttg
    10321 acctttttta ttatgtcgcc agtgatcgac aaaatttatg tcgatgcgta ccagccattc
    10381 agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc gctgcgtgag
    10441 tttatgctgc gtcagacccg cgaggcagat ttagggttgt ttgccagact ggcgaatacc
    10501 ggcccgttgc aggggcctga agctgtaccg atgcgcattt tgctcccggc ctacgtgacc
    10561 agcgagttga aaaccgcatt tcagataggc ttcacgattt tcatcccttt tttgattatc
    10621 gacctggtga ttgccagcgt gttgatggca ttggggatga tgatggttcc ccccgccacc
    10681 attgctctgc cctttaaact gatgctgttt gtactggtgg atggctggca attgctggtc
    10741 ggttcgctgg cgcagagctt ttacagctag agaggcaaaa tgacacctga atcggtcatg
    10801 atgatgggaa ctgaagcgat gaaagtcgcg ctggcactgg cagccccgct gttgctggta
    10861 gcgctggtca cgggccttat catcagtatt ttgcaggcag ccacgcagat taacgaaatg
    10921 acgctgtcgt ttattccgaa aatcatcgcc gtatttatcg ccatcattat tgccggaccg
    10981 tggatgctca atctgttgct ggattacgtc cgcaccttgt tcactaacct gccgtatatc
    11041 atcgggtagc ccgtacaatg atgcaggaga caagcgatca atggctttcc tggttaagcc
    11101 tgtacttctg gccgttactg cgcgtgctgg cgctcatttc taccgcgcca attctgagcg
    11161 aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg ttcgccattg
    11221 ccccatcatt acctgccaac gatgttcctg ttttttcgtt ctttgctctg tggctggccg
    11281 tgcagcagat cctgatcggc attgcgcttg gttttaccat gcaatttgcc tttgccgctg
    11341 tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct gtcatttgcg acatttaacg
    11401 gtcatttatg gttgatttca ctgctggtcg atacctttca caccctgccg attggtagcg
    11461 aaccgttgaa cagcaatgcg tttctggcac ccaccaaagc agggagtttg attttcctta
    11521 acgggctgat gctggcgtta ccgctcatta ctctgttgct gaccctgaat ctggcattag
    11581 gtttactgaa tcgtatggcc ccgcaattat ccatttttgt tattggattt ccattaactc
    11641 tgactgtcgg catctcttta atggcggcat taatgccgtt aattgcgcct ttttgcgaac
    11701 atttattcag tgaaattttt aatttgctgg ctgatattat tagtgaatta ccattaatat
    11761 aattccgtaa cgtttatcat gctaccctaa ggattatccg aaaaataata tctgcgaaca
    11821 tcttccagga tactcctgta gcgaaatatt tgttttaagc tcactcacat atcgcaacat
    11881 ttactttaag acaattccag gcaaattata caacacttta cgggatagta agtccgcctg
    11941 aaaaattgcg agagtggcgc attaggtgac ccatgttgtt ccgtttagtc atgatgaaat
    12001 attcaggtaa ggggaattat cgttacgcat tgagtgaggg tatgccatgt caacgattat
    12061 tatggattta tgtagttaca cccgactagg tttaaccggg tatctgttga gtagaggggt
    12121 taaaaaaaga gaaatcaacg acattgaaac cgttgatgac cttgccatag cttgtgattc
    12181 acagcgtcct tcagtggtgt ttattaatga ggactgtttc atccacgatg cttctaacag
    12241 tcagcgcatc aagcacatca ttaatcaaca tcccaatacg ttatttatcg tttttatggc
    12301 aattgccaat gttcattttg atgaatatct attggtcaga aaaaatttat tgatcagttc
    12361 taaatcgatt aaaccggaat ctctcgacga tatccttggc gatattctga aaaaagagac
    12421 aacgataacc tcgtttttaa atatgccgac gttatcattg agccgaaccg aatcgagtat
    12481 gttgcgaatg tggatggcag gtcagggaac cattcaaatc tccgaccaaa tgaatatcaa
    12541 agccaagacc gtttcatcgc ataaaggtaa tattaaacgt aagatcaaaa cgcataataa
    12601 acaggttatc taccatgtcg tccgactgac ggataatgtg actaatggta tttttgtcaa
    12661 catgcgctaa cacattctga ctggtggttt cccaccagtc aggctgaata agattactct
    12721 gctttctcaa caaagatacc gtcctgatgc cctgcttcat taaagaacca gatgccgagc
    12781 gggtagtctt ccagcgcaac caggtacatt gtgccttcac taaactcctc aactgccagt
    12841 accactccag gacgacgcgg accgctatcc gttttgactg ttacccgatc attcaccttc
    12901 atcgttttcc tcctgtggct ttgtgccagt gtagaacaat ttcgtttttc tggcagcgcc
    12961 aagcgcgcgc gagtgctgat tttctcgacg gtctatactt aagagatgcc aggcggactt
    13021 aacgactggc ggcaacaaca gagtaacggt tgcgaggaaa gatgatgaaa accgcaaaag
    13081 tgtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt cgatgccctg ctggcggcga
    13141 tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa cgattctgaa cacgttagcg
    13201 tcgatggacg tgaatatcat acatggcgtg aattggcgga tgccttcgaa ctggatattc
    13261 atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc ccgaccctga
    13321 gggtgtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt acaccaggaa
    13381 atctgatgtg ttcatcacct tatctgcaat ttttttcgct gacaagaaaa tattcgcgct
    13441 atgaatgatt aacgctactt atgaatagaa atgtgacgcg cgtcacccct tggtggctct
    13501 ggtgtcaacg ggatcgctcc cgaaaaaatc atctttgatt atttgtgctg ttttgagaat
    13561 catcctggta aggggagtac tgcgcgacca tgttttcaat tcaacaacca ctactggttt
    13621 ttagcgatct tgatggcacc ctgctggaca gtcatagtta tgactggcaa ccggcagccc
    13681 cctggctcag ccgtttacac gaagccaata ttcccgtcat tctctgtagc agtaaaactt
    13741 cagcggaaat gctgtacttg caaaaaatgt tggggctaca aggcttaccg ctgattgcag
    13801 agaatggcgc agtgatccag cttgctgaac aatggcagga tatagacggt tttccacgca
    13861 tcatctcagg tattagccat ggcgaaatct gccaggtttt aaatacgcta cgcgagaaag
    13921 aacattttaa attcacgact tttgatgatg tcgacgatgc aaccattgcc gaatggacgg
    13981 gattaagccg tagctaggcg gcgctgacgc agctgcatga ggcgtcggta acgctaatct
    14041 ggcgcgacag tgacgagcgt atggcacaat ttatcgctcg tctgaacgaa ctgggcttgc
    14101 agtttatgca gggtgcgcgc ttctggcacg tactggatgc ctctgccgga aaagatcagg
    14161 ctgccaactg gattatcgcg acctatcaac aattgtcagg cagacgccca accacacttg
    14221 gcctgggcga tgggccaaac gatgcgcctt tactggaggt aatggattac gcggtgattg
    14281 tgaaagggct aaaccgtgaa ggggtacatc tgcatgatga ggatccggcc cgcgtctggc
    14341 gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct ggaccatttt ttctccgccc
    14401 gttaagcgtg atcgctcgcg aatacccgat tgcgcccggc ctgttttgcg aggtaaagcc
    14461 gacggtcggc caatgactgg agttgttcaa aatcataatc accggtttcc tcgctgctac
    14521 ttacccccag cgaggcactg atgcgtatcg ttgtactctt ggcgatcagc atctcttttt
    14581 catttaactt caggcgaata cgttctgcga cctccgcagc ctgcgtcaga ttcgcgcctg
    14641 gcagaatcac acaaaactcc tcaccaccga cccgcccggc aacgtcctgc gcacgcaagg
    14701 aactgctaat taatccggca gcatgagaaa gaacacggtc gcccgcctga tgaccaaagc
    14761 ggtcattaat cgctttaaaa tggtcaagat cgacctgaat gacagaaaaa ggatgttggt
    14821 gcgtctgaca caatttagcg agcggacggg ctttttcgaa cagtgcgcca cggttatata
    14881 aacgcgttaa ggtgtcgtgc cacgcctgcc actgcaacga gctttgcaga acatacatgt
    14941 tgctgaccat ccggcgaatc acataccagg aaaggagtaa catggtggta aagagcgccc
    15001 acagcagggt taatgcaatg ctgatactgc cgaaatcgcc gcgcacgcct tcgcttagcg
    15061 tatggacgcg cgccagcaca ccgtcgaaat ggtccagacg ttcccagcta acatagcgac
    15121 tgtccatacg aatgccgccc cgcgtgtcat gttccattgc ctgcgccagc aaggccagtt
    15181 cacgaggatc aaaaatattc cctgttggat ggtcaggatt ggaagaggtc aaaaatatca
    15241 gcttactgtc atagagctga tactcaccat cgaggttttt atcgatggcg tttcttaaaa
    15301 attgctgcat agtacgcacg ggaatactca tccccagcac gccataccag tagttattac
    15361 tgtctaccgg aacactgacg gtaacctgcg gttcagtatt gctggcgtgt tccggttgcg
    15421 aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg ataaaccagg
    15481 gttgggtgac atagccgtaa taacgcgttg gtacattgcg cataaacaag gtcggctgcg
    15541 tcgaaacgta aaatccagca cgcgagacat acatcgcctg ttcaaccatc gacgaggagt
    15601 tgtgcgccag tcgcagcaag taaccaactt ccagtgcagc ggtaatttca ttgtcgaggc
    15661 tttcattttc gcgagacagg aaattcccct cgctgactaa tgcatccgat acgccattga
    15721 ctggcagggt gcgccgtcgg ttgagttcga tttgccaggc gtgctcgtcg cgatgctgtt
    15781 cgaactcggt tacggcatta cgcagagagg tgaaatccag tggcgccacg agagcttcgc
    15841 gcatgccatt acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc ttatcgagcg
    15901 cgttggctac gttttccaga tgattacgct ggctggagat ataggcatct tccagcacca
    15961 ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac cacgataaag cagagattaa
    16021 cgacatgacc gggccccagg cggcgtgcga gttttttcaa ccagctctgg ttttccattt
    16081 ttgtctcgtg ctgcaccctg acgactccca acctgctttt ttatgattct ggcataaccc
    16141 ggcaatagcg t
//
LOCUS       DAJTYF010000087        16067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTYF010000087 DAJTYF010000000
VERSION     DAJTYF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.087"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(270..974)
                     /locus_tag="OO590_002874"
     CDS             complement(270..974)
                     /locus_tag="OO590_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6638174.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1111..1563)
                     /gene="moaE"
                     /locus_tag="OO590_002875"
     CDS             complement(1111..1563)
                     /gene="moaE"
                     /locus_tag="OO590_002875"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6638175.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1565..1810)
                     /gene="moaD"
                     /locus_tag="OO590_002876"
     CDS             complement(1565..1810)
                     /gene="moaD"
                     /locus_tag="OO590_002876"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6638176.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1803..2288)
                     /gene="moaC"
                     /locus_tag="OO590_002877"
     CDS             complement(1803..2288)
                     /gene="moaC"
                     /locus_tag="OO590_002877"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6638177.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2291..2803)
                     /gene="moaB"
                     /locus_tag="OO590_002878"
     CDS             complement(2291..2803)
                     /gene="moaB"
                     /locus_tag="OO590_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6638178.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2825..3814)
                     /gene="moaA"
                     /locus_tag="OO590_002879"
     CDS             complement(2825..3814)
                     /gene="moaA"
                     /locus_tag="OO590_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6638179.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3802..3946)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4211..5119
                     /gene="yvcK"
                     /locus_tag="OO590_002880"
     CDS             4211..5119
                     /gene="yvcK"
                     /locus_tag="OO590_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6638180.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5311..7332)
                     /gene="uvrB"
                     /locus_tag="OO590_002881"
     CDS             complement(5311..7332)
                     /gene="uvrB"
                     /locus_tag="OO590_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6638181.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7911..8588)
                     /gene="bioD"
                     /locus_tag="OO590_002882"
     CDS             complement(7911..8588)
                     /gene="bioD"
                     /locus_tag="OO590_002882"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6638182.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8581..9336)
                     /gene="bioC"
                     /locus_tag="OO590_002883"
     CDS             complement(8581..9336)
                     /gene="bioC"
                     /locus_tag="OO590_002883"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6638183.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9323..10477)
                     /gene="bioF"
                     /locus_tag="OO590_002884"
     CDS             complement(9323..10477)
                     /gene="bioF"
                     /locus_tag="OO590_002884"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6638184.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10474..11514)
                     /gene="bioB"
                     /locus_tag="OO590_002885"
     CDS             complement(10474..11514)
                     /gene="bioB"
                     /locus_tag="OO590_002885"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6638185.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11601..12890
                     /gene="bioA"
                     /locus_tag="OO590_002886"
     CDS             11601..12890
                     /gene="bioA"
                     /locus_tag="OO590_002886"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6638186.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12868..13338
                     /locus_tag="OO590_002887"
     CDS             12868..13338
                     /locus_tag="OO590_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6638187.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13892..14893
                     /locus_tag="OO590_002888"
     CDS             13892..14893
                     /locus_tag="OO590_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6638188.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14927..>16067)
                     /locus_tag="OO590_002889"
     CDS             complement(14927..>16067)
                     /locus_tag="OO590_002889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR6638189.1"
                     /translation="YDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFD
                     NVMVNEVETAYLMQGLWHSKFIACQAGTCRVGLHFLGQCVSVSVSSCHFSRGNYSADE
                     SFGIRIQPQTYAWSSEAVRSEAIILDSETMCIGFKNAVYVHDCLDLHMEQLDLDYCGS
                     TGVVIENVNGGFSFSNSWIAADADGTEQFTGIYFRTPTSTQSHKIVSGVHINTANKNT
                     AANNQSIAIEQSAIFVFVSGCTLTGDEWAVNIVDINECVSFDKCIFNKPLRYLRSGGV
                     SVTDCYLAGITEVQKPEGRYNTYRGCSGVPSVNGIINVPVAVGATSGSAAIPNPGNLT
                     YRVRSLFGDPASSGDKVSVSVVTINVTRPSPVGVALPSMVEYLAI"
BASE COUNT         3817 a         4231 c         3997 g         4022 t
ORIGIN      
        1 ccgtttaata tcctggcctc gtgctgaaaa tctaatttat tgctgttttg cgagtatgat
       61 tccattcttt tagttgtcct tagaaaaaat attaatattc accatcaata aatttaaaac
      121 cacgcataca aaatactttt gtttttatgg atacggaaaa cgattccgct cgctcataag
      181 gcatatttta tacctggtac ttgttaagtg tcgtttttag taagaaaaaa atcccgacac
      241 atggccggga tagtgatggt gaaaaagaat taacggcggt tgccgaagat ccgtaacaac
      301 atcaggaaca ggttgatgaa gtccagatac aaggttaacg cgccaagaat ggaatatttg
      361 cgcaggttcg acgtgtcgcg ggtatcaatc tgctcaccca tatttttcaa tttctgagtg
      421 tcatacgccg tcaatccgac aaagacaatc acgccgatgt aggtaactgc ccacatcaat
      481 gcttcgcttt tcagccagaa gttgaccagc gatgccagca caatgccgat taacgccata
      541 aacagcatat tgcccaagcc acttaaatcg cgcttcgtgg tgtaaccgta caggctcatt
      601 gcgccgaaca tcccggcagt aacgacgaaa gtactggcga tagaagcagc ggtatagaca
      661 atgaatatac tggaaagcgt aagacccgtc agcgccgaat aaagcataaa gagcatcgtc
      721 gttacacctg cgctcagctt ttgaatcatc gctgataaca caataaccaa tgctaattgc
      781 gcgatgatca gaccgattaa aaagacacgg ttagtgaaca acagctccat cacggccgcg
      841 gaattagccg cataccaggc aacaaatgcg gtcagcaaca agccaacggt catccagcca
      901 tagacttgcg ccatataagt ttgcaagcca gcccggggtt gtacgattga atcagaacgt
      961 gggaatctgt ccatgacgat ctcctgaaga tataaggaat atcttaagga tactgcaaaa
     1021 tgatgaggct gtgcatcgac gcagcgtaaa cgcatgtact gagcggtgaa attgccggac
     1081 gcagcggtgc cttatccggc taacaaaaaa ctaccagcgt tttgccgcct gcagatcgct
     1141 ctcccgggct tcaacccagc ggtcgccttc cggcgtggct tcgcgcttcc agaacggtgc
     1201 gcgggttttg agataatcca taataaactg cccggcttca aacgcactgc tgcgatgcgc
     1261 actggtgaca ccgacaaaaa cgatttcatc gcccggccat aattccccga tgcggtgaat
     1321 caccgtgacg cgccccagcg gccagcggtt acgcgcttca tcaacaattt ctgccagtgc
     1381 tttttcagtc atccccggat agtgttcgag ggttaatgct ttgacgctgt cgcccaggtt
     1441 atggttgcgc acctttccgg tgaaggtgac taccgcaccg tcttcgtcac gctccgccag
     1501 ccacgggtac tcttctccta cgctgaacgg ctgcgggccg acaacaattt tggtttctgc
     1561 catcttaacc tccggttacc ggcgggagga aagctacttc gtcgccgtca gtcagcggat
     1621 ggtcaaaact caccagcgtc tggttgacgg cagccagcaa tttgccatct tccagcgcca
     1681 gcgcccagcg atcgctctgc gcagccaggt gctggcgtaa cgcttcaacg gttgggtaat
     1741 ccgcagccac ttcggttgca tctgttccca ccaactcgcg cacctgggcg aaaaaaagaa
     1801 ctttaatcat ccgcttccac cttaaagtca cccgacttgc cgccgctctt cgccagcaaa
     1861 cgtaccggac caatcaccat atctttttgc accgctttgc acatgtcata aatggtcagc
     1921 gccgccacgg aggccgcggt taacgcttcc atttcgacac cggttttccc ggtcaggcgg
     1981 cataaggttt ctatacgcac ccgattgtgc tccggctcgg cctgtaaatt gacttcaact
     2041 ttgctgagca tcagcgggtg acacagcggg atcagatccc aagtgcgttt tgccgcctga
     2101 ataccggcaa tacgcgcagt ggcaaatacg tcgcctttgt ggtggcgacc atcaataatc
     2161 atcgccagcg tctcgctgcg catggtgaca aaggcttccg cccgcgcttc acgcacggtt
     2221 tccgctttgg cggagacatc caccatgtgc gcttcgccag cggcgttgat atgggtcagt
     2281 tgcgacatac ttatttcttc aaatgtggat ggaaattaca cggacgcgta cgggcatcca
     2341 gctgcggcgc gatgatattt tcccatgcgg tacggcacgc tttggtcgaa cccggcatgg
     2401 cgaaaatcag cgttttgtta gcgacgcccg ctaccgcacg agattgcaac gtggaagtgc
     2461 caatctcttc aaacgacaac atacggaaca cttcaccaaa accttcaacc tcacggtcga
     2521 acaacggcag cagcgcttcg ggagcctgat caccttccgt caggccagta ccacccgtaa
     2581 tcaataccac ttgtacatcg tcgctggcga tccacgcaga tacctgagcg cgaatagcgt
     2641 agcggttttc tttcacaatg gctttatcga caacgtgatg gcccgcttct tgcgccgaat
     2701 cgcgcagata gtgaccggag gtatcgtctt cttcaccgcg acgattagaa accgtaagaa
     2761 tagcaatacg ggtcgggata aattcagtgc ttacctgact catctgatct ctccttttga
     2821 cgttttagcc gccaatgtac gataagtttt gcgtaatacc ggtgttgttt tgatgcagga
     2881 aatgggtctg tttcttctcc cgcagcgccg ctgaaatacg cgcttccagc gcctgttgct
     2941 gggtatcgtc ttccagcaga tcgcgcaggt taacgccgcc ttcaccaaac aggcagagat
     3001 ggagtttacc aatggaggaa acgcgcaggc ggttgcaagt ggcgcagaag tctttttcat
     3061 acggcatgat aaggccaatc tctccggcgt aatctggatg gcaaaagact tgcgcgggac
     3121 cgtcgctgcg ttgacgtaat tggtggatcc agccgcgacg cagtagctcg tcacgcagaa
     3181 cctgaccaga gatgtgatgc ttacggaaga gctcgctgcc ctcgcccgtt tccatcagtt
     3241 cgatgaaacg cagctggata gggcgatgct ggatccagtt cagaaaggtg tcgagctggt
     3301 gatgattaac atcacgcatc agcacggtat tgactttgac cttctcaaaa ccggcctcaa
     3361 atgcagcatc aatccctgcc atgacctggt tgaatttatc ctgcccggta atagcgtgaa
     3421 actggcgggc gtccaaactg tcgacactga cgttaatgcc agtaagtccc gcatcgcgcc
     3481 agttcgccac atcgcgttcc agacggtaac cattggtggt gaccgcaatc tggcggatag
     3541 cgtcgttttc ccgcacagcg gcgatgatat cggtaaagtc gcggcgtaaa gacggctctc
     3601 ctcctgtcag gcgcactttt tcggtgccca ggctggcgaa ggcgcgcgta acccggcgaa
     3661 tttcatcgac ggtaagaaag cctttattgg tgacgccgct cggtttgtag ccatccggca
     3721 ggcagtaggt gcaacgaaag ttacacacat cggtaatcga caggcgcaag tagtaaaact
     3781 tacgcgcaaa tgcatcagtc agttgtgaag ccatgtacac ctttccagat acgggaggcg
     3841 aagtcatttc ttccttcgcc ctggtggcaa tctttacgag cattgtcacg gccaaagcac
     3901 cgtatcagtt gacccaggtg cagaggctag agtgtttagt ggttaagccg atactagcgt
     3961 gtaaatgaga tttttaccat ccacactttc gctatgcaat catgtatata gcgtcatgat
     4021 cgtgctattt cgctacagaa taagggaaaa tggcggtttt actttgatat acatcatcag
     4081 ggaggcatag cgtgcatcgt cctttagaga tagaagaaaa tagtttgtat cctcaatatt
     4141 ggcaggttaa ttgctgtttc cccgcaattt gcgctactgt agcgcggata atctgactcc
     4201 aggaaactat atgcgcaatc gtacgctggc tgatcttgat cgtgtcgttg ctctcggcgg
     4261 agggcatgga ctgggacgcg ttctctcatc actttcgtct ttgggttctc gtttaacggg
     4321 tatcgtcacc accacggata atggtggctc gacggggcgt attcgccgtt cagaaggcgg
     4381 cattgcttgg ggcgatatgc gcaactgcct caaccagctg ataacggaac cgagcgtcgc
     4441 ctccgcgatg tttgaatacc gttttggcgg caatggcgaa ctttccggtc ataatctcgg
     4501 aaacttgatg ttaaaggcgc tggatcacct tagcgtgcgg cctctggaag ccatcaattt
     4561 aattcgtaat ctgctgaaag tggatacgca tttgattcca atgtcagagc atcctgttga
     4621 tctgatggcg attgacgatc aggggcatga agtttacggc gaggtcaata tcgaccagtt
     4681 aactacgccg attcaagagt tattgttaac gcctaatgta cccgcaacgc gtgaggcggt
     4741 tcacgctatc aatgaagcgg atctcatcat tattgggcct ggcagttttt ataccagcct
     4801 gatgccaatt ctgctgctga aggaaatcgc ccaggcatta cgccgcacgc cagcgccgat
     4861 ggtttatatc ggcaatctgg ggcgtgagtt gagtttacct gcggctaatt tgaagctgga
     4921 aagcaagctg gcaattatgg agcagtatgt tggtaaaaag gtcattgatg cggtcatcgt
     4981 cgggccaaaa gtggatgtct cggcggtgaa agagcggatt gtgatccagg aggtactgga
     5041 ggccagcgat attccgtatc gtcatgaccg ccagttgtta cataacgcgc tggaaaaggc
     5101 gttacaggct ttaggttaac ggaaatccga aggaaaattc cggcttccta ttgaagacaa
     5161 agtgcgcgtt gtttatgccg gatgcggcgt gaacgcctta tccggcctac aaaccgcgca
     5221 aattcaatat attgcggaga aaatgtaggc ctgataagcg tagcgcatca ggctgttttc
     5281 cgtttgtcat cagtcttctt cgctatcctg ttacgatgcg gcaataaaca actcacgcaa
     5341 ctgatgcaac tggtcacgaa tttgtgccgc ttcttcgaac tccagattct gcgcgtgttg
     5401 catcatcagc ccttccagtt catggatttt ctgctgcaac gctttcggcg acatatccat
     5461 cggcacatta tccggctcaa caatcgggcg cgattttcct ctgcccttcg ctttggtttt
     5521 ggcaatgttc tgccccagcg ccaggatatc gaccactttc ttgttcaagc cttgcggcgt
     5581 aatgccgtgt tcctcgttgt acttctgctg tttctcacgg cgacgttcgg tttcgccaat
     5641 cgctttcgcc attgatggag tgatcttatc gccgtagaga atcgctttac cgttaacgtt
     5701 acgtgccgca cgaccaatgg tctggatcaa cgaacgttcg gaacgcagga agccttcttt
     5761 gtcagcgtcg aggatcgcca ccagcgacac ttccggcata tccagacctt cacgcagtaa
     5821 gttgatccct accaatacgt cgaactcacc cagacgcaag tcgcggataa tctccatacg
     5881 ctcgacggtg tcgatatctg agtgaagata acgcacgcgc tcaccgtgtt cttcgagata
     5941 ttcggtaaga tcttccgcca ttcgcttggt cagtgtggtg accagcacgc gttcgttaat
     6001 tgccgctcgc tgacgaatct ccgaaagaag atcatcaacc tgtgtcgcca ccggccgcac
     6061 ttcgataatc gggtcaagca atccggttgg acgcaccacc tgatccacca catcgccgcc
     6121 ggatttttcc agctcgtaat tacccggcgt cgccgaaaca tagatggttt gcggcgctaa
     6181 tgcttcgaac tcttcaaact taagcggacg gttatccagc gctgatggca ggcggaagcc
     6241 gtactccacc agtgtctctt tacgcgcccg gtcaccgcga tacatgccgc caatttgtgg
     6301 aatggtgacg tgagattcat cgacgaccag cagcccatcg gcaggcaggt aatcaaacag
     6361 cgtcggcggt ggctcacccg gtccacgacc ggagaggaag cgcgagtagt tttcaatccc
     6421 cgaacagtag cccagctcgt tcatcatctc cagatcaaac tgggtacgct gggtcagccg
     6481 ctgctcttcc agcagtttgt tgttttccaa cagcactttg cgtctggcgg ccagttcttc
     6541 tttgatctcc tccatcgcct gtacgatgcg ctcgcgcggt gtgacgtagt gcgttttcgg
     6601 gtagatggta aaacgtggaa tagtggaaac aatctgcccg gtcagcgggt caaataacga
     6661 caatcgttcc acttcctcgt caaacagttc cacgcgaagt gcaatgtcat ccgattctgc
     6721 cgggaagata tctatcacct cgccacgaac gcggaaagta ccacgctgga atgcttgatc
     6781 attacgagcg tattgcagct ccgccagtcg gcgcagaatc gcgcgctgat cgataatcat
     6841 accgaccgtg agatggagca tcatcttgag atataaatca ggatcgccca gaccataaat
     6901 cgcggaaaca gacgccacta caatcacatc acgccgctcc agcatcgctt tggtggcgga
     6961 caaacgcatc tgctcaatat gttcgttaac cgaggcatct ttctcaatga aggtgtcgga
     7021 actcggtaca taggcttccg gctgatagta gtcgtagtag gagacgaaat actccaccgc
     7081 gttttccggg aagaactctt tcatttcgcc atacagctgg gccgccagcg ttttgttggg
     7141 cgcaagtacc atggttgggc gctgaaggtc agcaatgaca ttggcaatgg tgaaggtttt
     7201 cccggagcca gtcacgccaa gtaacgtctg gtgcgccagg ccatcttcca gcccctcttc
     7261 gagacgtcga atcgcctctg gctgatcgcc agaaggttta aaagcggaat tcagtttgaa
     7321 cggtttactc atgagtcgct acctgaagga gttgggcggg caggtatgta attttactcg
     7381 tcgtacttaa ttatgccaac aaattatact ggataaaaaa acagttcatc accataatat
     7441 ttctgataca gcgtaaactc cgctgtcaat catgagcaaa atttactctg tggcgagata
     7501 aaactccggc cttaccgggt tatccccaaa gcaacggctt ttttaacatt tgtcaagatg
     7561 agtgatgaca gttttatggc aagagatgcc tgtacagtga ctgccattgc atttatctaa
     7621 ccagttaaaa aataaaagat atttctttga gccgtcttta acgccaattc gcgtgcaagc
     7681 cgcgtattct ctcgcttgcc tcgtgttttc taactctaat acacatggtt atccacagga
     7741 atagtggata actgtctcca gcccctatcc cccgccgcct gggcaattcc cacattcgcc
     7801 gcatgaggcg gaagtaaaat ttttttcgtg attattttag aatttaattg gttaaattgc
     7861 agtcaatcga agacgcaatc tcgctcgcaa tttaaccaaa tacagaatgg ctacaacaag
     7921 gcaaggttta tgtactttcc ggttgccgca ttttctggat tttctgcaag ccaggggatc
     7981 tctcccagca gcggcgcggg aatcatgcgg gtgagcgtgg tcatatattc agcgtgacgt
     8041 tttcccggag gcgtaacatc gttcgccacc caacccgcca gagtcagtcc ggcgtgttgt
     8101 attacctgcg cagtcaacat cgcgtgatta atacagccga gtttcacacc aactaccagt
     8161 atcaccggca gttgttcctg tgttacccaa tctgcaaaag tgaaagtgtc agaaagcggc
     8221 gtaaaccagc cgccagcacc ttccactaac acccagtcag cctgttgttc aagcgcgcgt
     8281 aatccggcgc tcattaccaa tgattctatc ggcctgccct cttgggcgct gatgatgtgc
     8341 ggcgaggtgg gttctgcgaa ggtgtaagga tttaccgttg cgtaatccag ctgcaggctg
     8401 ctgttgtgct gtaacgccag cgcgtcacta ttacgtaagc cttccggggt cttttcgctg
     8461 ccagaggcga ccggtttata acctgccgtc cgataaccta ctgccttcgc ggcttgtaaa
     8521 agtgcacaac tggcgacggt tttccccact tcggtatccg ttccggtgac aaaataacgt
     8581 ttactcacga gcaatcactc ccaaaaaaag atgatacgtc agaggatatc gcccctgttg
     8641 ttgcggccag gccagttgca atcgctgcaa ctgcgaacgc gttaatattc gcgggtcgcg
     8701 cccttcatga agatgcgtag caccgatgcc tttcagcgaa cgcatggcac tgagcgcatc
     8761 atcaaaccat agtgtgatga gctgaatatg atgttgataa cgcacgccgt tcagcgactc
     8821 ttcgatttca tctggcggta aaaagcgatt agcatgcgga cgctcgtcca ccgcctgccg
     8881 cgcctgatgc agttcgggta acgatccctg caccagcgtg gtaaacgcga ccacgcctcc
     8941 ggggcgcacc acccgataca gctcgcggag tgccgtggat aaattaccgc accactgcac
     9001 cgcgagattg ctccatgcaa gatcgaacgt cgcagtcgct aacggcaggg attcgatatc
     9061 tcccgccaga taatgatctg cggcatcttt atggcgggct tgcgcaagca ttggcggcga
     9121 gagatctaac gccgtcacct gcgcgtgacg ttcccgccag tggcggctca tccagccagg
     9181 tccacaaccc gcgtccagta cgtgggtgta tttacgctgt ggaagcattg ccagtaaggc
     9241 gtcagcactc tggcgctgta gatctgcatg ttgctcatag tgtgcggctg cccgaccaaa
     9301 tgccgctgca atggcttgtt tattaaccgt tgccatgcag cacctccagc agacggtcga
     9361 tatcctgcat ttcatgcgca gcggttagcg ttaagcgaag tcgcgcagta ccagcgggta
     9421 cggttggcgg gcgaatcgcc gtgacccagc agccttgctg gcgcagtttt tctgccagtt
     9481 gtaacgcacg gctgttatca ccgacgatca atggctggat ggcgctgcat gaatcagcca
     9541 gcgtaaacgg caaatcctgt actccggcac gaaaacgcgc aatgagtgac accagttttt
     9601 cgcgccgtgc atccccctca tcacggcgaa tgaccgccag cgacgcacgt aatgcctgcg
     9661 cctgagcggg cggcatactg gtgctgtaga taaggtggcg ggcgaattgc agcagataat
     9721 ccgccaccgt acttgagcaa agcaccgcag ccccactgac gccaaatcct ttgccaaaag
     9781 tcactaccag caattctggt tttacctttt gcagccagca gctgccgcgc ccctgctccc
     9841 cgataacgcc cgtgccgtgg gcatcatcga ccatcaacca gccattgtgc tgttgcgtta
     9901 cctgctggat ttccgccagt ggcgcgctat cgccgtccat gctgaacacg ccttctgtca
     9961 ccaccagttg ctgccccgga cagggggaag caagcagtcg cgccagatga gcgacatcgt
    10021 tatgagtaaa acggcgaagc tgcgacgggc ttaaactggc agcctccagc aatgaggcat
    10081 ggctaagccg gtcggcaaca atacggtcct ctttcgccat catcgcggca atgactgcct
    10141 gattcgcggc aaaaccagag ataaacagca gcgcccgcga atagccaagc cactcggcca
    10201 gttcttcttc cagcgcctga tgcaccacgc tataaccgct gacgtgaccg gagccgccgc
    10261 taccgatgcc aaattgctcc gccccctgct tccaggcacg gataatttgc ggatgatggc
    10321 ttaaacctaa ataatcgtta ctggaaaagt tcagatactg gcgatcatcc gccaccagcc
    10381 agcgtccggc tccttgcgcc accggataac ggcgacgcag ggcatcggca gcacgccgcg
    10441 catcgagcgc cgcgttgatt ttctcctgcc agctcataat gctgccgcgt tgtaatattc
    10501 gtcggtgtcc ggggtcatca gcgcctgtcc aagacgttgc tgttgttcgt tatcccctgc
    10561 cagcacggca gtttgctgcg gatttagccc cagtttgcgg aacagttgca ggtctttatc
    10621 ttcttccgga ttcggcgtgg tcagcagttt gcaaccgtag aaaatcgagt ttgcgcctgc
    10681 cataaagcac atcgcctgag tctgttcgtt catctgctcg cgtccggcag aaaggcgcac
    10741 gtaagaggtc ggcatcatga tccgcgctac ggcaatggtg cgaataaaat caaaggcatc
    10801 aacgtcatcg ttatcggcaa gcggcgtgcc tttcaccttc accagcatgt tgattggtac
    10861 gctttccggc ggcgtcggca ggtttgccag ttgcagcaat aatccggcgc gatcttttac
    10921 cgtttcgcct aagcccacaa tgccacctga gcagactttg atcccggcat cgcgcacttt
    10981 ttccagcgta tcgaggcgtt cctgataagt gcgtgtggtg atgatattgc cgtaaaactc
    11041 cggcgaggtg tccaggttgt ggttgtagta atccagcccg gcgttcgcga ggcgctgcgc
    11101 ctgagattca ctcaacgtgc ccagcgtcat acacgcctcc agccccatcg cttttacccc
    11161 ctgcaccatt tgttccaggt acggcatatc gcgttcgtgg ggattcttcc atgccgcgcc
    11221 catacagaag cgcgtcgatc ctgccgcttt cgctttgcgc gccgactcca gcacctgttc
    11281 aacttccatc aaccgctcgg cttccagccc ggttttgtag cgcgagcttt gcgggcagta
    11341 tttgcaatct tccggacaag ctccggtctt aatcgacagc aacgtgctga cctgcacctg
    11401 acgaggatcg aaatgctggc gatgcacctg ctgcgcttca aacagcagat ccagcaacgg
    11461 tttttcaaat aattctgtga cttgcgacaa tgtccagcgt gggcggtgag ccatggggct
    11521 tctccaaaac gtgttttttg ttgttaattc ggtgtaggct tgtaaaccta aatcttttca
    11581 atttggttta caagtcgatt atgacaacgg acgatcttgc ctttgaccaa cgccatatct
    11641 ggcacccata cacatccatg acctcccctc tgccggttta tccggtggcg agcgccgaag
    11701 gttgcgagct aattttgtct gacggcagac gcctggttga cggtatgtcg tcctggtggg
    11761 cggcgatcca cggctacaat cacccgcagc ttaatgcggc gatgaagacg caaattgatg
    11821 ccatgtcgca tgtgatgttt ggcggtatca cccatgcgcc cgccatcgag ctatgccgca
    11881 aactggtggc aatgacgccg caaccgctgg agtgcgtttt tctcgcggac tccggttccg
    11941 tagcggtgga agtggcgatg aaaatggcgt tgcagtactg gcaagccaaa ggcgaagcgc
    12001 gccagcgttt tctgaccttc cgcaatggtt atcatggcga tacctttggc gcgatgtcgg
    12061 tgtgcgatcc ggacaactca atgcacagcc tgtggaaagg ctatctgcca gaaaacttgt
    12121 ttgctcccgc cccgcaaagc cgcatggatg gtgaatggga tgaacgcgat atggttggtt
    12181 ttgcccgcct gatggcggcg catcgtcatg aaatcgcagc ggtgatcatt gagccgattg
    12241 tccagggcgc aggcgggatg cgcatgtacc atccggaatg gttaaaacga atccgcaaaa
    12301 tgtgcgatcg cgaaggtatc ttgctgattg ccgacgagat cgccaccgga tttggtcgta
    12361 ccggcaaact gtttgcctgt gaatatgcag aaatcgcgcc ggacattttg tgcctcggta
    12421 aagccttaac cggcggcaca atgacccttt ccgccacact taccacgcgc gaggttgcag
    12481 aaaccatcag taacggcgaa gccggctgct ttatgcatgg gccaactttt atgggcaatc
    12541 cgctggcctg cgcggcagca aacgccagcc tggcgattat cgaatccggc gaatggcagc
    12601 agcaggtggc ggctattgaa gtgcagctgc gcgagcaact ggcaccagcc cgtgatgccg
    12661 aaatggttgc cgatgtgcgc gtactggggg caatcggtgt ggtcgaaacc actcgtccgg
    12721 tgaatatggc ggcgctgcaa aaattctttg tcgaacaggg ggtatggatc cggccttttg
    12781 gcaaactgat ttacctgatg ccgccctata ttattctccc gcaacagttg cagcgtctga
    12841 ccgcagcggt taaccgcgcg gtacaggatg aaacattttt ttgccaataa tggcgataag
    12901 ttgccgcatc gtcatgtctt taacggcatg ggttacgatg gcgataatat ttcaccgcat
    12961 ctggcgtggg atgatgttcc tgcgggaacg aaaagttttg ttgtcacctg ctacgacccg
    13021 gatgcgccaa ccggctccgg ctggtggcac tgggtagttg ttaatttacc cgctgatacc
    13081 cgcgtattac cgcaagggtt tggctctggt ctggtagcaa tgccagacgg cgttttgcag
    13141 acgcgtaccg attttggtaa aaccggatac gatggcgcag cgccgccgaa aggcgaaacc
    13201 catcgctaca tttttaccgt tcacgcgctg gatgtagagc gtattgatgt cgatgaagga
    13261 ggcagcggcg cgatggtcgg gtttaacgtt catttccact ctctggcaag cgcatcgatt
    13321 accgcgatgt ttagttaatc actctgccag atggcgcaat gccatctggt atcacttaaa
    13381 ggtattaaaa acaacttttt gtctttttac cttcccgttt cgctcaagtt agtataaaaa
    13441 agctgaacga gaaacataaa acgctgtaaa tatcaatatg ttatgaaaga tttagtctaa
    13501 ataatagact gcataatact acaaaacaca acatatccag tcactatgaa tcaactactt
    13561 agatagtatt agtgacctga ggcagagcat tagcgcaagg tgattttttg tcctcttgcg
    13621 ctaatttttt gtcaacggac tgggttagtt gaatttacaa ccatgctaag tatctcgata
    13681 agcgcagaga aatgatgcag tggtgggcgg attggcttga tgagaaggtt aggtaggcaa
    13741 atccatgcct gagcagagcc ttgccctcta cctgagccca tgctcattct ttggcctcaa
    13801 cgcgagcatg acctcactgg ctggacccaa aatggactat gacagaaaga tctgatattt
    13861 ttcctcgcaa aaacttagag gctttatttt aatgcataag agcaactgct ttgacacggc
    13921 aaggcttgtg gcagctatga tggttctggt cagtcaccac tacgcgctct ctgggcagcc
    13981 ggagccgtac ctgttcggct ttgaaagtgc tggcggcatt gcagttatca tattcttttc
    14041 aatatcagga tatctgattt caaaatcagc tatcaggagt gattccttta ttgatttcat
    14101 ggctaaaaga gcgagaagaa tattcccggc gctggtccct tgctctatac tgacatattt
    14161 tttatttgga tggatactta acgacttcag cgcagaatat tttagccatg acattgtcag
    14221 gaagaccata agctctattt ttatgagtca ggctccagat gctgacatta catcacattt
    14281 aattcatgcc ggaattaacg gcagtttgtg gaccctaccg cttgagttcc tgtgttatat
    14341 aataacggga gtagcagttg ccctactaaa gaacggaaaa gcattcatag ttatactgtt
    14401 ggtttttgtt tctctttcat taattggaag tgtgtcagaa aacagggatg taatgttctc
    14461 tattccgtta tggctgtatc ctttgcgtgg actggcgttc ttttttggtg ccactatggc
    14521 aatgtatgag aagtcatgga atgtaagtaa tgttaaaatt actgtagttt ctttgcttgc
    14581 tatgtacgcc tacgctagtt atggcaaagg cattgattat actatgactt gctatatcct
    14641 ggtttcattc tcgacaatag ccatatgcac atccgttggc gacccactgg ttaagggcag
    14701 gtttgattac tcgtatggtg tgtacattta tgcattcccg gttcagcagg ttgttataaa
    14761 cacattgcac atgggttttt acccgtcaat gttgttaagt gccgtgactg ttcttttctt
    14821 gtcccatctt tcatggaatc tggttgaaaa aagattcctg acacgaagtt cgcctaaatt
    14881 atccctggat tgaacaataa aaggggccat atggccccta aaataatcag atggccagat
    14941 actcaaccat tgaaggcagc gcgactccta ctgggcttgg gcgagtaaca ttaattgtca
    15001 ccacggaaac actaaccttg tcaccacttg atgcagggtc accaaaaagg cttcttactc
    15061 tgtatgtcag gttccccggg ttcggtatag ctgcagatcc gcttgtggca cctaccgcaa
    15121 ctgggacatt aatgatccca tttacagacg gtacgcctga acagccacga tacgtattgt
    15181 atctgccttc cggtttctgc acctcggtaa tgccagccaa ataacagtca gtaactgaca
    15241 cgccaccgct acgaagatag cgtagaggct tattgaatat gcacttatcg aaagaaacac
    15301 attcattgat gtcgacaata tttacagccc attcatcacc agttaacgta caaccactta
    15361 caaagacgaa gatcgccgac tgttctatcg ctatactttg attgtttgca gccgtgtttt
    15421 tgtttgcagt gttgatatga acaccactga caattttatg tgactgcgtt gaggtcggcg
    15481 ttctaaaata tatccccgta aattgttcag tgccatcggc atctgctgct atccatgagt
    15541 ttgagaaaga aaatcctccg tttacattct ctataaccac gcctgttgaa ccgcaataat
    15601 ctaaatccag ttgttccata tgcaaatcaa ggcaatcatg aacatagacg gcatttttaa
    15661 aaccaatgca catggtctca ctgtctaaaa ttattgcttc tgacctaact gcctctgacg
    15721 accacgcata cgtttgaggc tgaatcctga tgccaaagct ttcatcagca gaataatttc
    15781 ctctgctgaa atggcaggag ctgacactaa cacttacgca ctggcctaaa aagtgaagac
    15841 cgactctaca ggttccagcc tgacatgcta taaattttga gtgccagagc ccttgcatta
    15901 aataagcggt ttcaacttca ttcaccatta cattatcaaa tacccagcca taaacctgtc
    15961 tgatgacaga gtcttctgct gtaatgtttg tgtcaatacc attgcaggtg ttattaccag
    16021 ttactcgtat atttctcaga taacggccga tagtgtctga atcataa
//
LOCUS       DAJTYF010000088        15952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTYF010000088 DAJTYF010000000
VERSION     DAJTYF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.088"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="OO590_002890"
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="OO590_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6638190.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(998..2020)
                     /locus_tag="OO590_002891"
     CDS             complement(998..2020)
                     /locus_tag="OO590_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6638191.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNLVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="OO590_002892"
     CDS             complement(2034..3536)
                     /locus_tag="OO590_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6638192.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDMREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLNTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="OO590_002893"
                     /pseudo
     CDS             complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="OO590_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4943..5473
                     /gene="ppa"
                     /locus_tag="OO590_002894"
     CDS             4943..5473
                     /gene="ppa"
                     /locus_tag="OO590_002894"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6638193.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5853..6194)
                     /locus_tag="OO590_002895"
     CDS             complement(5853..6194)
                     /locus_tag="OO590_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6638194.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="OO590_002896"
     CDS             complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="OO590_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6638195.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="OO590_002897"
     CDS             complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="OO590_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6638196.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11912..12550
                     /gene="msrA"
                     /locus_tag="OO590_002898"
     CDS             11912..12550
                     /gene="msrA"
                     /locus_tag="OO590_002898"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6638197.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12873..14216
                     /locus_tag="OO590_002899"
     CDS             12873..14216
                     /locus_tag="OO590_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6638198.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14278..14484)
                     /locus_tag="OO590_002900"
     CDS             complement(14278..14484)
                     /locus_tag="OO590_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6638199.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14809..15363
                     /locus_tag="OO590_002901"
     CDS             14809..15363
                     /locus_tag="OO590_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6638200.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPRSQFIVDSNGVARGAWQLDDESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15426..>15952)
                     /locus_tag="OO590_002902"
     CDS             complement(15426..>15952)
                     /locus_tag="OO590_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638201.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
BASE COUNT         3798 a         4373 c         3920 g         3861 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac caccaggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcaa atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcaaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttct
     3001 tgggtgttga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 atgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct taacggtcaa
     4141 tggcgacgct ggccccaacg gtgccctgca gctccaccac gttgcatggt ttgccattca
     4201 cttcttttac cagccagtca ccgatcaact taccttcgag gatgttgtcg gcagtaacgg
     4261 tggtcatata gagagatttg tctttcacat cgatggaacg gtcgagcaag aagaccggga
     4321 tttcggcatc tttcgcctct tttaataccg gttcccaacc cgtcgcaacc actggagcga
     4381 taaagatcgc atccacccct tgtgcaacga aggaacgtac cgctttaatc tggttttcct
     4441 gcttttgctg accatcggca attttcaacg taattccgcg tttttcggcc tcacttttcg
     4501 ccacattggt ttctgcggcg cgccagccgg attccgatct gacctgcgaa aatcctacgg
     4561 ttaatggagc ggccaacgcc atagacgaca tggctgccga gactgcagag actacaagta
     4621 agcgtttcca catagggttg tcctcgtcgg ggtaattatt attggtgaaa agatgttcgc
     4681 gaaaaaacta tagacaattc gttatgtaac ggattgcgtt acatcacact tcaaaaaagt
     4741 aaataaaacg ttaatcacaa gtttgtaatc gctttcatct cactatgaaa aatgcggcta
     4801 cggttatgga ttttcctgct ctgtataccg tcttaaaact ggcgaaaaag gaaaatgaag
     4861 acgaaaacaa gcaaagacat tcggcgcgag ttggctataa tacttggcac ttgtttgcca
     4921 catattttta aaggaaacag acatgagctt actcaacgtc cctgcgggta aagatctgcc
     4981 ggaagacatc tacgttgtta ttgagatccc ggctaacgca gatccgatca aatacgaaat
     5041 cgacaaagag agcggcgcac tgttcgttga ccgcttcatg tccaccgcga tgttctatcc
     5101 gtgcaactac ggttacatca accacaccct gtctctggac ggtgacccgg ttgacgtact
     5161 ggtcccgact ccgtacccgc tgcagcctgg ttctgtgatc cgttgccgtc cggttggcgt
     5221 tctgaaaatg accgacgaag ccggtgaaga tgcgaaactg gttgcggttc cgcacagcaa
     5281 gctgagcaaa gaatacgatc acattaaaga cgtaaacgat ctgcctgaac tgctgaaagc
     5341 gcaaatcgct cacttcttcg agcactacaa agacctcgaa aaaggcaagt gggtgaaagt
     5401 tgaaggttgg gaaaacgcag aagccgctaa agctgaaatc gttgcttcct tcgagcgcgc
     5461 aaagaataaa taagttcttc tagcgcaata accctgaacg ccgggcttcg gttagtaagg
     5521 gtttttttat gcccgcgata aataaactct ctattccacc atcattattc tcagcggttg
     5581 caaggcttga acggtaagaa caagcaaacc cgaccaccat tttgctgttc atagccactt
     5641 gctggaagtt agccgacctc actcatactc accgttcaaa gcgtgccccc gttttacccc
     5701 tgcacatttg tgaacgccag cagagttttt tatgcgattg cagatatgga aagcggctcg
     5761 gagagaaaag caaaagatgg aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa
     5821 caacgccacc cgaaggaggc gtgcttttat tattacttat ccctgtctaa ccagtcgccg
     5881 ctttcaatta gctttaatcc atcgacgggt cgttgataaa cgtacatcca tgcactccca
     5941 tacggcgtct gaatcaactg gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt
     6001 tcggccagcg tggcgttgtc aatacgataa acttcaccgt gtaccgttcc gttccccgga
     6061 actgcgcctg gatagtggcc caggctatac aactggtagt tatcgatact gaaatcgccc
     6121 agtaactggg cattggtcat ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag
     6181 acaaatattc gcattgctaa aactcgaact gatagagcaa atccagtgcc tggtctacac
     6241 cagacacggc ttccagatat agcttaggca tcaggcgata acgtaacgtg agtgttgcta
     6301 tagagtcaaa tatacccacg ccgtatttca cttgcagacc tggcaataca tagccgctga
     6361 ccactacctg ggaggagtcg cctactccca gggtgtcgag cgctaaattg cttacgccaa
     6421 acgtctcgcc gattttaccc acaatctggc cactttgcgc aacccccaga ccaatcagca
     6481 tcgaggtcat tgccgcactg tcgctctgat cgctctccag cccttgtcca cgtagcaaat
     6541 aagacaatgc agcttgttgc gacatcgccg ggtcagagaa gatctccgct ttcggttcgt
     6601 ccgccagacc agtgacgcga actccggcga ttacatcgtc ttctgtagca tccgggttac
     6661 gaatagcttc aatattaaga tacggttgat ccggcggacc agagaacagt aactcaccct
     6721 tacgcacaat cagatcctga ccataggcat ggaagcgccc ttcagggatg ttgatctgcc
     6781 cgttcaggcc cagcccttgt ttgtcctgta cgacgttaag atcgcccgtc agccgcgctt
     6841 tcaggccaaa ggcgtcaatg cgcacattgt tgccaacgtg gacaatcagg ttactgttaa
     6901 tcggaatcga cgccgttttc ggctcttccg gttgcaggtt atcgttaagc atcaccacat
     6961 cgctggagac gcctactgcg ctttccggca gatcgtgcac cacgatgcgc gcccacggga
     7021 catccacgcg accatcgagg gtaaacaggt ttggtgtagc ctcgaataca acatctggcg
     7081 atacatccat tcgtaccatc ggcggcacgg tgatccgcac tttactgcct ttcgccgtta
     7141 ctcgtgcccg ccagttttca atttggctcc agtcggcatc accgttcagg tagatttcac
     7201 cctgctgggt ccgtactgta ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa
     7261 gctggctcgg ctgcatatca aacggcataa agttgccgtc gatatccaca cccgtaacct
     7321 gaagctgacc aaacaactgc gggctttgca catcaccacc cagacgcaag ttggcactca
     7381 ccatccccgc tgctttttcc ccacgggtaa agatggggtt tatcatcgca aggttgaagt
     7441 tacggatatt gacgttgcca ccaagattac ggcggccttg cggatcggtc acctgcacct
     7501 gtccatcaaa ctggccgtta ttggtcaggc ggatggtcca gcccaattcg gcacggttgt
     7561 tacgcaattc cgccgtcaga ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga
     7621 cggtttgcgt tacctgcacg ttacgccccg aaagggtgat actgccctgc ggcagcccct
     7681 ctttcgtggt gtcccaggca acatccgctt tacccgtgaa gataccgctg gcctgagtgg
     7741 tttctggcat aaacggtttc agcatggcga ggtcgaagcg gttgagattc accaccgcac
     7801 gcccttcggc ccccgcatcg atagtttgcg gcacgcacag ttccgcattc gggttaagcc
     7861 agcagtgtgg cccgatgctg attttttgct ccttattgcg gtaatccagc gcaatatcgc
     7921 gggtcagcga ccacgggccg accggcgtct ggaagcgggt attgctaaga gttcccttcc
     7981 agcgttcttc tttgcgatca aaacttcctg ccagattaag ctgcccggag acaggctcgc
     8041 cctgaatccg caactgtagc tcgtgctgct tttcgctgcc tttggcattc agggtgacga
     8101 ggttgatatt tacatccggc tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga
     8161 tctgatcggt ggatttaatg tcgccttcca cgcgaacctg cgccacggaa agttcctgcc
     8221 agcgcaggcc gcgcgcggtg atatctgcca gtagttgtgg cgcttccacc gtgccgcgta
     8281 ctttcaccag ccctttcgct gtaccgccaa gccccggcag cgcgttatcc agccccggcg
     8341 cgttgatggt ggcatcaaga ttgagatctt ttacccccag ctcgcctttc acttcggcac
     8401 tgtttggccc gagttccaga tgaagcccag ggatcatcca ctgcatataa ctgttgcctt
     8461 tcagcgtgcc gtcaacgttc actttgttct gtttgacgtt accggtcagc ttcagttctg
     8521 gcacctccat ctgccaggtg ccgccgtaca ggctaccgcg ggttttaatc aagccattga
     8581 gtttcgacgg ccactccggg aactctttgg cggtgtaaat gccattaagc gttagctcac
     8641 cgcgccaact aatggcctgc tgccagtcga gcaacgcctt gagttcagtt ttcccttcca
     8701 gcgccgcgac ggtgagtttg tcgagattga cctgctgttc attacctttg gcatcaaggg
     8761 taatggtggc tggcgggatc tcctgtccct tcactgccgt acgcatagag agcgtgtaat
     8821 cggtcatttt gccggtaagt ttcagtttca gatcatccgc ctgatactgc ttctcaccag
     8881 tgaacggcca gtaaagctgt ttgctgttca cttccacgtt gagtggcaat ccggcttccg
     8941 ccagtcgcgt ctgggcgcgt aaatccatat ccaccggacc ggaaaggtta acgcctatct
     9001 ccagctgttc gcgcagcgcg ccgcccactt tcagcttcac cttttcacct ttcaacggct
     9061 ccacgttcag tgtgctgttg agggtgatat ccaccggcca gttgtctgac agctgcgccg
     9121 tgccgctggc gttgacgatc ccctggttgg aatcgatatc caaggcgtcc agtttagtat
     9181 tgccgtcaat gctgctcact ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg
     9241 tcacgcgcag ctgctcgccc ttaaactctt caatgttcag gttaagcggc aaatgcacgt
     9301 cggtcatttc cggcaatacc gggcgagaaa agagatcttt cagcgtttcg ccgagcggct
     9361 tttcctccgg ctgcggattt tcaattttcg gttcgaccac ttcttcctgc gccacttcca
     9421 ccactttcgg cagagcaatc agcaggcctt tcagcgacgt cggtttcagg gtcagggttt
     9481 tctcctgcca gttcaggccg gaggtgaagt ccatcaccga caccgtggtg tcatcaatct
     9541 tgatgttgac gttgtccagc gccacccgtg tcagggtgat gggatacggc gtggagagat
     9601 ccagcggacc gctatcttcc tcttcttcaa cctgttcaga aggaggcatt tttttactgt
     9661 cgatgttgac ctgaatgtct ttcagcgcca ggtcattaat acaaacgcta ctgttccaca
     9721 ggcactcaag accgaccgcc agatgcagat tacccgcttt taccgccacg cctagctgct
     9781 cataacgaac gtcagacaag gtgagatcgc gccagccgcc ggtgaccttg ccaatatcca
     9841 gtcctggcac ccagcgatcc gccgctttaa atacaagatg caggccgctg gtggtgccca
     9901 ccagaaacgc caccgatccc agcaacagta agataacgat aaccacgccg aggctgattt
     9961 ttttccataa actcataatt ctggccccag accgatgtaa aactgtaacc cgtgttcgtc
    10021 tttatccgcg accggtacgg caaaatcgag tttgattggc ccgaccggcg attcccagcg
    10081 tacgccgacc ccggtaccgg ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc
    10141 actatcgaca aacaccgcgc cccaccattt tccggtcacg ttgtactggt attccagcga
    10201 tccggttatc aacttcgagg cccctttcag gtcaccgttg gcgtatttcg gggcgataga
    10261 tttgtatttg tagccgcgaa tactgcgatc gcccccgacg aagaaacgca gatccggcgg
    10321 tactttgtcg aaatcaccgg tttcaatcca gcccagcgta ccgcgtgtca caaagcgatg
    10381 gcgatcgtac agtgtgcgga tccagacgtt ctgcgcctgg aaaacggaga aatcgacatc
    10441 tgaaccccag gccgtgttgg agtagtcgat agagtagcgt tgcgagtctc cccaggttgg
    10501 catcaggcca ccacgagaac gcgtgcggct aatcatcacc ccaggataaa acagcatcgt
    10561 ggtgttggta atttcaccct gggtaaagtg gtcgaggctc cagcgcaggt taatggcacg
    10621 ctgccagccg ctggagagat cccagtagcg agaagccacc agcgtagtgg agtcagattc
    10681 ggtatcgttc aggtcagtgc gcttaaaacc gccctgcacc aaataatatt gttccagtgg
    10741 attcttcagc agcggcattt tatagctgaa gtcgagggtc tgttccggcg cggaaatact
    10801 ggtactggtg gtcaaactgt gaccgtatga gttcatccac ggctttttcc acgtcgcttt
    10861 cacgcgcggt cccacgtccg tagagtaacc gcccccggtt tcgatggtgt tttctgttcg
    10921 cggcgaaacc acgcccgtca atggtaatac tttcgtttcg cgcgctttat caaattgtgg
    10981 agccaccacc accgagttaa accagccggt agcagaaagt cggcggttca gttccgccaa
    11041 atctttcgat tcatactcat cgccctcttt aaacggcacc agattttgca ggtattcatc
    11101 gcggatttgt gatccttcaa aggtcacatg cccaaagcgg taacgttcgc cactgttata
    11161 atcaatatcc cagaaggctt tatgcaggcc gagcgcaatg cccagctgcg ctttggtaaa
    11221 ttcgctatcg aaataacctt tacgcaacgc aatactggtt aaggactttt tgaaattttc
    11281 ataatcgccc tggttcagta ccgtaccaat agccgggcga gtatcgagca atttcaaata
    11341 gtctttatcg gtccgcgcgc cgccgcgcaa taccacatcg gtgccgccaa ttaacaccgg
    11401 cacacctggc gtgactttgg cgatcaatac ctgccgccct ttctttggcg gtggacggag
    11461 atcaaattca atggtcggct ggtaataacc cagcgctttc agaccttcac ggatggcatc
    11521 atcgacgcgt gcgcgaaagc gacggtctgg cgtcacttca tcactttcaa tcgtagaaag
    11581 ctgcgcacga acgttctttt ccagctgtcc cgataacccc tcgacctgta gacggacgtt
    11641 cgcggcgacg gcagatccgc ttaagcagag taagcttaca cagcataact gtcggatata
    11701 gcgcacattt tctcctgaat atcctttttt cctgcccctg gaacgccgtt aaacggctta
    11761 acaaaaatcc agtaatatgg attaaaaagc agactaaacc ccaaatattt cttatgtttt
    11821 actttagacc tattcacggt ggttattgtg tgcaaatacg cctcttgtta caaccttaac
    11881 cccaatgacc gattttcggg agagcgacac catgagttta tttgataaaa agcatctggt
    11941 ttcccccgcc gatgccctgc ctggacgtaa caccccgatg cccgtagcca cgctgcatgc
    12001 ggtcaacggt cactcaatga ccaatgtacc tgacggaatg gagattgcca tttttgcgat
    12061 gggttgtttc tggggtgtgg agcgtctgtt ctggcagtta cccggccttt acagcaccgc
    12121 cgcaggctat accggcggct atacgccaaa tccgacttat cgggaagtgt gctccggtga
    12181 tacgggtcat gccgaagcgg tacgcattgt ttacgatcct tccgtcatca gctatgagca
    12241 gttgctacag gtattttggg agaatcacga tcccgcccag ggaatgcgtc agggcaatga
    12301 ccacggcacg cagtatcgtt cagcgattta tccgctgacc ccagaacagg atgccgcagc
    12361 tcgcgccagt ctggaacgtt ttcaggcggc gatgcttgcc gccgatgacg atcgtcacat
    12421 caccacggaa atcgctaacg ccacaccgtt ttattatgcc gaagatgacc accagcaata
    12481 tctgcataaa aacccatatg gttactgtgg aattggcgga attggcgtct gtctgccgcc
    12541 ggaagcatag cgttacgggt acaaatgtag tttgttgata aagtgctctt tgtctatgcc
    12601 tgatgcggct taaacgactt accaggccta caaaatcgtc caaattcaat atattgcaag
    12661 gactgcgtag gcctgatcgg catagcgcat caggcagtat tgcatttatc agcagtctga
    12721 attttaaccc tctggcgact ttacagtacc ttacgctata ctagcgactg aaaatgccgg
    12781 ttcactttct tcgaatcggc tttcaatgtg tatttcacac aaattaatca actttccctt
    12841 ccgaggatct ggcctgaaag gtcggataag atatgttaaa cagtatttta gtcatactct
    12901 gcttgatcgc tgtaagtgcg ttcttctcga tgtccgagat ctcgcttgcc gcctcacgca
    12961 aaatcaaact taaactgctg gctgatgaag gcaatataaa tgcccaacgc gttctgaata
    13021 tgcaggaaaa tcccggcatg ttctttaccg tggtccaaat cggtctgaac gcagtggcga
    13081 ttctcggcgg tatcgtcggt gatgcggcat tttctccagc ttttcacagc ctgttctccc
    13141 gctatatgtc ggcagagctc tctgagcaac tgagctttat tctctctttc tcgttagtga
    13201 ctggcatgtt tatcctgttt gcggatttaa ccccgaaacg catcggtatg attgcgccag
    13261 aagcggtggc tttgcgtatc atcaacccga tgcgcttctg cctgtacgtt tgcaccccgc
    13321 tggtgtggtt cttcaacggc ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg
    13381 tacgtaaaga tgacatcact tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg
    13441 cgggcgtgtt acgtaaacag gaacacgagc tgattgaaaa cgtctttgag ctggaatccc
    13501 gtaccgttcc gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag
    13561 atgagcaaag cctgaagaat aaggtggcgg aacatccgca ctctaagttc ctcgtctgta
    13621 atgaagatat tgaccacatc atcggttatg tcgattctaa agacctgctg aaccgcgtgc
    13681 tggctaacca aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc
    13741 cggatacgtt aaccctttca gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg
    13801 cggtgatcat gaacgagtac gcgctggtgg tggggatcat caccctcaac gacgtgatga
    13861 ccacgctgat gggcgatctg gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg
    13921 agaactcatg gctgattgac ggcggcaccc caattgacga cgtcatgcgc gtgctggata
    13981 ttgacgagtt cccgcagtcg ggcaactacg aaaccatcgg cggctttatg atgtttatgc
    14041 tgcgtaagat cccgaaacgc accgattcgg tgaaattcgc cggctacaaa tttgaagtgg
    14101 tggatatcga taactaccgc atcgaccagc tgctggtgac ccggatcgac agcaaggcca
    14161 ccgccctttc gccaaaactg cctgacgcta aagataaaga agaaagcgtc gcgtaacccc
    14221 cagaaacatc aacggctcct gaatcaggag ccgtcttatt actgcatagc actttggtta
    14281 agccatctct gtttgcagac gcataacctg acggttaact tcggacatca ctgacaaatg
    14341 cagcttatcc ttcacttttg ggataagaat cttaccctta tcaaattcaa aagcgccaac
    14401 gtccttgatg tataaccgtc cacggaacag gatctttacg tacttcgcca cctgaagtgg
    14461 gttgtaccgt tggaaaattt tcattgttat ctcctgctga gtattacgcc tgtgcggtgc
    14521 cacaatcggc ccaactatta tgaggcgcaa attttaatgc ttagtgacta tagactatcc
    14581 gggcaatgtt tccaccgcgt ataacttttt ttacctaaag gttacaatta ttcagaatta
    14641 tctttttacc gaagcgcggt cttcagtata agcattcatt tttcatatga tttgtgcgct
    14701 tgaccgcaaa ctggcatcac acttgcggga aattcgataa atagcacata tgattaaaac
    14761 tcagacccaa gtggtcggat cacctgcata tcataagaag gaaacaccat gaccctacgc
    14821 aagattctgg cactcacctg cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa
    14881 atcggtcagc gagtgccgcc gattggcatt accgatcggg gcgagttggt gcttgataaa
    14941 gatcagttta gctacaaaac ctggaacagc gcgcagttag tgggaaaagt gcgagtactg
    15001 caacatattg ctggtcgcac ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt
    15061 aaatcagcga agttaccgca cgatcgttac cagaccacca ccattgttaa caccgacgac
    15121 gcgattccgg gttccggcat gtttgtgcgc agcagtctgg agagtaataa aaagctttat
    15181 cccaggtcgc agtttattgt cgatagcaat ggcgtcgcgc gcggtgcctg gcagctggat
    15241 gacgaaagtt ccgctgtagt ggtgctggat aaagacggtc gcgtgcaatg ggccaaagac
    15301 ggggcgctca caccggaaga ggtgcagcaa gtgatggacc tgctgcaaaa gttgctcaaa
    15361 taatggcaat aacataaatt ctggaaaaat aattttcccc tatcgttatc tatgggatag
    15421 gggaattaag tctctgagtt cttacaccga actaatactg gtaacgctag ttgctctttt
    15481 ttaaacgcct caatgatcga tttatatttc tcgcaaatct tttctttggt aatattacta
    15541 tctgcggcca gattcgcact tactatcgca tccacttgtt cggctaattc gacttgttgg
    15601 caacctgcat taaccagaaa attataaata agagagtaat cttcatcctc caaaacaaca
    15661 gaggaaccat tacgaaaacg ttcatcccct ccatagattt taagcgcaaa gcccaaagat
    15721 tctatgacta tggcaaaatt atcatcaatt attttttcgt aaatatacca tccatcacat
    15781 tctttaattc caccgcctgg tatttggagt tcaaatttac cccgttttaa tttgcttact
    15841 acatcctcca ataaggcata accgaaagat acgtcaccaa acgcctggcg aatataacct
    15901 ttaatccttt ttggtatgtc agactgtttt ttctgcaaaa tatcacaggc at
//
LOCUS       DAJTYF010000089        15914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTYF010000089 DAJTYF010000000
VERSION     DAJTYF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.089"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..396)
                     /locus_tag="OO590_002903"
     CDS             complement(<1..396)
                     /locus_tag="OO590_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6638202.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTED"
     gene            complement(393..3077)
                     /locus_tag="OO590_002904"
     CDS             complement(393..3077)
                     /locus_tag="OO590_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6638203.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            complement(3169..3669)
                     /locus_tag="OO590_002905"
     CDS             complement(3169..3669)
                     /locus_tag="OO590_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6638204.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(3699..4415)
                     /locus_tag="OO590_002906"
     CDS             complement(3699..4415)
                     /locus_tag="OO590_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6638205.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            4711..5487
                     /gene="exuR"
                     /locus_tag="OO590_002907"
     CDS             4711..5487
                     /gene="exuR"
                     /locus_tag="OO590_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6638206.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            5832..6494
                     /locus_tag="OO590_002908"
     CDS             5832..6494
                     /locus_tag="OO590_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6638207.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            6498..6881
                     /gene="mzrA"
                     /locus_tag="OO590_002909"
     CDS             6498..6881
                     /gene="mzrA"
                     /locus_tag="OO590_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6638208.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            7028..7382
                     /locus_tag="OO590_002910"
                     /pseudo
     CDS             7028..7382
                     /locus_tag="OO590_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            7420..7725
                     /locus_tag="OO590_002911"
     CDS             7420..7725
                     /locus_tag="OO590_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6638209.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            7728..8132
                     /locus_tag="OO590_002912"
     CDS             7728..8132
                     /locus_tag="OO590_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6638210.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            8122..8421
                     /locus_tag="OO590_002913"
     CDS             8122..8421
                     /locus_tag="OO590_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6638211.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            8607..8999
                     /locus_tag="OO590_002914"
     CDS             8607..8999
                     /locus_tag="OO590_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6638212.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTTLFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            9069..10055
                     /gene="yqjG"
                     /locus_tag="OO590_002915"
     CDS             9069..10055
                     /gene="yqjG"
                     /locus_tag="OO590_002915"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6638213.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            10348..10716
                     /locus_tag="OO590_002916"
     CDS             10348..10716
                     /locus_tag="OO590_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6638214.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(10923..11819)
                     /gene="yhaJ"
                     /locus_tag="OO590_002917"
     CDS             complement(10923..11819)
                     /gene="yhaJ"
                     /locus_tag="OO590_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR6638215.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            11924..12625
                     /locus_tag="OO590_002918"
     CDS             11924..12625
                     /locus_tag="OO590_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6638216.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12648..12812
                     /locus_tag="OO590_002919"
     CDS             12648..12812
                     /locus_tag="OO590_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638217.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(13024..14316)
                     /locus_tag="OO590_002920"
     CDS             complement(13024..14316)
                     /locus_tag="OO590_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6638218.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(14344..15675)
                     /locus_tag="OO590_002921"
     CDS             complement(14344..15675)
                     /locus_tag="OO590_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6638219.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTCWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT         4146 a         4045 c         3682 g         4041 t
ORIGIN      
        1 atcttctgta catgaagatc tattagaatc cttaagattc gtctgaagcc ctttgccccc
       61 cttgcatcct agcggcgctt catcgctact tacaatggct gttttaaatc ctttgagttt
      121 taccggccaa cgcatcccac tgcgttgttc agttaaatag atttgtactg tcgatatatt
      181 tccattagct gtacaggttt gttggaggtc aggatacgaa cattcatacc acatatatcc
      241 attatttcct gccgacgctt catccttcca gaagtaataa tccgctctac cagatgccaa
      301 atcatacgta atttcactgg tcacgtcagg agctgcctga acgccagtga gcgtgccaaa
      361 caagcccaat atcgccgcaa tcaatcgatt tctcatggtg caacctccct ttgcgccgtc
      421 tgaacgtttc cacgtaattt aggctgtttc aactgtgcct gaacctgaat cgactccggt
      481 aattgtgaat aagtcaccac actacaagta acatctccgc tgtaacgaat gctggcgcgt
      541 ttctttacaa cactcagtgg acacttaagg aattgctgcc cggaaagcag atagagctct
      601 ttttcgctgc cgttaatttc aagtgtaaat ccgccatctt cagctaccag taacatggcg
      661 ctgttcagcc ccacaatcgg gaattttttc accgctgccg gcagtaataa acgaccgata
      721 tagttgtagt taaagctggc agacacttcg cgatagcgca gcttgccagg caacaacatt
      781 aattcacgat tccctgcacc gttaatgatg tttgttgtcc cctgctgatt gccctggcta
      841 ctgtcgttaa cctcaacaaa accagactga aatccaggat aagggaacaa cacactgtca
      901 ctttgaccaa gctgcaaagt tcggctgcct gccacccgcg cttcgagttg cagatcctga
      961 ctctcttcat cctgttcaga gaagcctttc acatccacca gtaccgcacc cagcaaatcg
     1021 tctgtaccac tggctcccaa attaacgcca tagcgactga cagccagcgt agagctataa
     1081 gtgcccgtaa aggccgaaag atgatcatga ttcttacggt cataactatc agatacggta
     1141 gcgtagacat ttccgtattg cccatcccaa cgcccgttaa ccgcgctgtt tatggtatcg
     1201 gtgttcaggc ctccgacggt tacgccaagt tctttatggc tgaatgaatc gttataaaag
     1261 gtatgcgata actgccatga cgtttgatcg ccatcctgtt cgctataacg ataatccgta
     1321 gacacattgg ttgaatggct gttgttattg ctatccatcg ttggcgtgtc agataacgaa
     1381 aacgataagt acaagccgtt gtcgtcgtta tcgccaccgt tgtcattacg atggaaaaca
     1441 ccgactgacg tactcaaaat ccagtcacgc atcacaatag cgcgggaagc gctaagttgt
     1501 acagtttcag agatactttg cgctgttgtc tggcgaagat agttattttc aaagctctgc
     1561 tgtttatccc aacgcaaact atcatcagtg ttattttgtg tgcaggtata acccagcatc
     1621 atattccagc cgacaaaatt cagtgaaact gtcgcggaaa tactttcata acagcttaag
     1681 gcgttatagc tctctctgct gtcggttgta caagcatcgc cgtcagagtt ggtccgataa
     1741 aagcccaacg tcggccatcc cggattactc cagttggcct gttgcatatc acctttgccg
     1801 ccgtcatcgt tacggaacac gctggcgctg attgcaagat tccccacccc gcctaaatct
     1861 gccgtccagt tattacctaa ctcgaaagca ctcacatcat cggcattcgc cagccctgcg
     1921 tagagctcat attgcggatg taatggcagg cgtactccta gctgataagc tgaactttca
     1981 tcatcagaaa cctgtgatgt agttttacct gcctgtaaga accattgcgc atttccgtca
     2041 gtcagaccgc cggttttggt aaacggcaca agctcggtgc gggtgagttg gttattttcg
     2101 tagactttta atgccacgct gtagctgcct ggcggaaagg aactggtatc aataaattgc
     2161 gaaccactat tgagataaaa cgatcccaaa agttgctcat tacgataagc gtcaacacga
     2221 gaattgcgcg aaagcagaac cattactggg gttccttgct ggctttgcgc ctggtttaaa
     2281 tagctcagtg tcgacccgat acgcatccca tcgattgcac cgagtggcag aaagttaaag
     2341 gtaaagttcc cgccttgtgc attaaatagt gtgcggttgt ccatgcgacc cgcctgcaca
     2401 taataacgac gtaaaaaatc ataacgataa tagagatcgc tgacgtcagt attactgtca
     2461 ctgacatcat ctgcatcata accgttgaaa ttccagtgtg caccaatata gctattttct
     2521 gttattccca gcgcaccgtt tccctgaata gacaacgatt ggtaatcatc ctgtgccaga
     2581 acattgatgt cttgctgatg tataaaagcg ttaacagtat ttttgtctgg atttaaatat
     2641 aatgacttcg catcgaaagc tgaattccat tgcggattaa taaacaacgt tgccgaactt
     2701 tcttcatcat tatagattat ttccagcgta tcggtattca gaaaaccaca ccctgagtca
     2761 gtacgtacat aaccgcaagc caattcaccg tggcgagcta gcggctgcga taatttttct
     2821 ttcactatat gcgcgatttt ctgatcgtct acatccagtt ccagcttgtt atatagactt
     2881 gctggatcaa gaaatgttac ggtatcaaga ttaaccattg cataatattt accgagactt
     2941 ttccctgaaa aataaacctc gatatactcc tgctgtccct gagcaataac ctcaaaccct
     3001 gcgggaattt tggttaatgt cgcctctgcc gggctgagac tcgccaggat caaaagagcc
     3061 agtaattttt tatccatggc aatcctgaaa tccataacaa atactaaaac agttaaaata
     3121 agggagggct acccctccct ttgttacgtg ttaattctgt tatccggatt aaatatcctg
     3181 agacagataa atgcttacta caccacggta gatgcctgtt tccagaacac ctttggtggt
     3241 ttgagcaaac atcaggtttt tagccagaga accgttagtt aacgcatcgg aagcaaagat
     3301 tttggttgcg gtaaaagtgg ttgccgcatc agctttaatt tcttcaccac cccaggttac
     3361 tgtcagcgga acaatcttgc tagcatccag gctttgaacc agttgcgctg gggacactaa
     3421 ctgcattttt acatctttgg taacgtcgtt agaccagatt ttggtcatca actggtaaga
     3481 ttgcagaccc tgccccggca gatattgcat ttctactgct ttcggcaacg cggtgttatc
     3541 ggtctgtgtc atatccagag cggcatcaac gttggccgtt acagtaatat ccttttgtac
     3601 tgccaatgcg gagcctgcaa cagaaaacat cgctgcgacc agaagagatt ttgcaaatac
     3661 ctttttcatt actattcctt cgtttattac aatgataatt aatggaccgc cagttcaaac
     3721 tcgccattat ctttggtgat ccagttatta tatttgacaa cgattttgtc gtagccagat
     3781 atgctttcaa gatttctttc ctgaccaggg aatatgtttt tatttacggt ctgtatctta
     3841 cagttttctt tgctattatt tttacaataa gcgatcgtta aaagctttaa gcgttgattc
     3901 cctgcattaa ttaatttttg ggcagaattt acttctaact tagctatagg ttgctgaggt
     3961 ggaacactaa caacgattcc ccaaattaag ttaactgtta actgcataga gagatctttt
     4021 ttatctgtgg caacgtcatc aatactggga accgcttcaa atttaacgcg ataatttttc
     4081 tctttttgct ctggttccat cgcaacaaaa cgtacggttt tgctactacc ggctggtaaa
     4141 gcgaatttag gtggcataac aaccagatga tttgcatcac cggatttaat ctcaacttca
     4201 ttttcctgag gcgtcgatgg attatcgata cggaatactg tcgccttgat gtactgaact
     4261 tcattacttt tagatattac gcgaacgttg ccttcaccct gactattaat ggaaacaggc
     4321 ataggataaa cagtcatatt tgactgtgcc aaaaaaggag cgaacacgaa taaaagcggc
     4381 gtaagccgta ataagagatt aattttcgcc accatataat tttgatctta caatatcgtt
     4441 tcaaaaaagt tggtgtatta gagatgtaga tttattgtaa gtcaatataa aaaagcattt
     4501 ttagttaaca ttaaatttaa aggctgggaa ttaacttact gaaatttagt agctttcaca
     4561 atgacgatac ttatttttta ggaatttata aatattcaca atattaattt aatacattaa
     4621 ttcaatcatc aaatttatat atgaccaatg gcatacccat gaataaagtg gtatgataac
     4681 ttcgtccgca ttccactccg ggagacgcat atggaaatca ctgaaccacg ccgtttgtat
     4741 caacaacttg ctgctgacct gaaagagcgc attgaacagg gcgtctatct ggtgggtgat
     4801 aaactgcctg ccgaacgctt tattgccgat gaaaagaacg tcagccgaac ggtagttcgt
     4861 gaagccatca tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg ttcaggtatt
     4921 catgtggttt ccaaccagcc gcgtcatcag caggcggctg acaataatat ggaattcgcc
     4981 aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa tattgccgag
     5041 ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat tcaggaacag
     5101 gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca cattcaggta
     5161 gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg gacccagcgt
     5221 agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac cgttgataac
     5281 tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc tcatgctgct
     5341 aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa cgaaaccagc
     5401 gacgatttcg agttcaatgc cgaccgctat ctgttcgccg aaaacccggt agtgcatctc
     5461 gatacggcca ccagcggcag caaatgaaat tcctgtccga caggcgctgc aatgcgcctg
     5521 tttgtctcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc tctcgccacc
     5581 ctcccctgca atcagcaaaa tcagactcca cggacatgta attttgataa cgaacaacgt
     5641 tgacctttgt tacaattaga ttcaatttga atttatgctt ttgaatgctt tcttatctca
     5701 cgttttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga acataccaaa
     5761 acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa ccgattcacc
     5821 aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg caggattttg
     5881 aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt gtaattttgt
     5941 tccttgaaaa tggcttgctt ccggcggcct ttttaccggg cgacagttta ctggtattgg
     6001 tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg ctgctgaccg
     6061 ttgccgccag cctcggttgc tgggtcagct atattcaggg gcgatggctg ggcaataccc
     6121 gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc gcacaccatc
     6181 tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgca tttgtcagaa
     6241 cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt cagttcttca
     6301 actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac atgctcggca
     6361 aaacgccggt atttttaaag tacgaggacc agctgatgtc atgcctgatg ctgctcccgg
     6421 tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa aagaaatatg
     6481 gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta gcctggtccg
     6541 gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt cgccagcaag
     6601 agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac ggtttttcga
     6661 tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc aaaaacgaca
     6721 ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg gtcctcgaca
     6781 gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag gctatgcagt
     6841 ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt atccgaaaca
     6901 tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg gtggttaaca
     6961 ctaccgcttc gttgtctgga caatcgttcc tttgtaatag gtccgaatca caatggaagg
     7021 ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta gtgccggtag
     7081 ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga tcagctatgc
     7141 cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga gtgaagtccg
     7201 ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg caaagcagaa
     7261 agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag gcgatgccga
     7321 tagaacggaa actggcagaa gcgcaggaag agctgaaaaa gctggaagcg cgcgactact
     7381 aactcacaat agtcactact tactcacctg gagaaaacta tgtcgaaaga acacactacg
     7441 gaacatctgc gtgctgagtt gaaatccctt tccgatacgc tggaagaggt gcttagctca
     7501 tctggcgaga agtcgaaaga agagttgagt aagattcgta gcaaagcgga gcaggcactg
     7561 aaacagagcc gttatcgcct gggtgaaacc ggtgatgcca tcgccaaaca aacccgtgtc
     7621 gcggcggcgc gtgctgatga gtatgtgcgt gaaaatccgt ggaccggcgt gggaattggc
     7681 gctgcaatcg gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg gcggacactc
     7741 atcacgcgca agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt gtttccatca
     7801 tggttgaaat ggtggaaaca cgtctgcggc tggcggtggt ggagctggaa gaggaaaaag
     7861 ctaatctctt tcaactttta ctgatgctgg gcctgacgat gcttttcgct gcatttggtc
     7921 ttatgagcct gatggtgcta attatttggg cggttgatcc gcaatatcgc ctgaatgcga
     7981 tgattgccac caccgtggtg ttgctgctac tggcactgat tggcggtatc tggacgttac
     8041 gtaaatcgcg taaatccacc ttgctgcgcc atacacgcca tgagctggca aacgatcggc
     8101 aactgctcga ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg taaggcgcaa
     8161 ctgcttagcc agatccagca acaacggctg gatctttccg ccagtcgtcg tgaatggctg
     8221 gaggcaacag gcgcttacga tcgtcgctgg aatatgctgc taagtctgcg ctcctgggcg
     8281 ctggttggca gtagcgtgat ggcgatctgg acgattcgcc atcctaatat gctggtccgc
     8341 tgggccagac gcggttttgg cgtatggagc gcctggcgtc tggttaaaac gaccctcaag
     8401 cagcaacagc ttcgcggtta acttccctct ggccggagca attccggcct tatccctcaa
     8461 attttttgaa tatttttgac agttttcctt gctaacaatc atcattcacc acgtttatga
     8521 ttctctccat cgacagcaac gacgctaata ccgcgccctt gcacaaaaaa acaatcagca
     8581 gcctgagtgg cgcagtggag agtatgatga aaaaattaga agatgttggt gtactggtag
     8641 cgcacatttt aatgccgatt ctgtttatta ccgcaggctg gggaaaaatc accgcttact
     8701 ctggtaccca acaatatatg gaagcaatgg gtgtcccggg ttttatgctg ccgctggtga
     8761 ttctgcttga gttcggtggt ggtctggcaa tcctattcgg tttcctgact cgcaccacaa
     8821 ccctgtttac tgcgggcttt acgctgctga cggcattttt atttcacagc aactttgctg
     8881 aaggcgtcaa ctcgctgatg ttcatgaaaa acctgacaat ttctggcgga ttcctgctgc
     8941 tggcaattac cggtccgggc gcgtatagca tcgaccgcct gctgaataaa aagtggtaag
     9001 cacgcgctat acttaacgat aaaaaacgag gaggaagctc ctcgtttttg ctattggagg
     9061 agagaaaaat gggtcaactg attgacggcg tctggcatga cacctggtac gataccaaat
     9121 ctaccggcgg taaatttcaa cgttcagctt ccgcatttcg taactggctc actgccgatg
     9181 gcgcacctgg ccccactggc acaggcggtt ttatcgcaga gaaagatcgt tatcatctct
     9241 atgtttcact cgcctgcccg tgggcgcacc gcacgctgat catgcgcaaa ctcaaaggac
     9301 tggaaccgtt tatttccgtt tccgtagtga acccgctgat gctggaaaac ggctggacct
     9361 ttgatgacag ttttccggga gcaaccggag atacgctcta tcaacatgaa tttctgtatc
     9421 agctttatct ccacgccgat ccacactaca gcggacgagt tactgttccc gtgctgtggg
     9481 acaaaaagaa ccacaccatc gtcagcaacg aatcagcaga aatcatccgc atgttcaata
     9541 ccgcgtttga tgcgctgggc gcaaaagcgg gtgattacta cccaccagcc ctgcaaacga
     9601 aaattgacga acttaacggc tggatttatg acaccgttaa caacggcgtg tataaagctg
     9661 gttttgccac cagccagcaa gcctacgacg aggcggtggc gaaagtgttt gaatcgctgg
     9721 cgcgactgga gcagatttta ggccagcatc gttacctgac cggcaaccag ctaaccgaag
     9781 ccgatattcg cctgtggact acgctggtgc gttttgatcc agtgtatgtg acccacttca
     9841 agtgtgataa gcaccgcatc agcgattacc tgaatctgta tggcttcctg cgcgatatct
     9901 accagatgcc ggggatcgcc gaaactgtca atttcgatca catccgtaat cattacttcc
     9961 gcagccataa gaccatcaac cctacgggga ttatttcaat tggtccgtgg caggatctcg
    10021 atgaaccgca tggacgagat gttcgcttcg gttaaaaaaa ggcaccttac gaggtgcctc
    10081 tgataattag ctaagtgcat gttgttcatg tcggatgcgg cgtgaacgcc ttatcaaacc
    10141 tacaaagcgt ttatattcaa tatgttacaa tgaccgtgta ggccagataa gcgtagcgca
    10201 tctggcgatt ttgcgcttgc catcagtctt agcgcgcctt cacggcactt tattaattcc
    10261 ttgaacgaat atttactgcc atttcattca cgtctattct taatttgctg ctttaaaaac
    10321 aagtgattga gcaaaattga ggcaaaaatg gactggtatc tgaaagtact aaaaaattat
    10381 gtcggtttcc ggggtcgtgc gcggcgcaaa gagtactgga tgtttattct ggtcaacatc
    10441 atctttacgt tcgtgctggg gctgctggat aaaatgttag gctggcaacg cgctggtggc
    10501 gaaggcatcc tgacgacaat ctacggtatt ctggtgtttt taccgtggtg ggcggttcag
    10561 ttccgccgcc tgcacgacac cgaccgctcg gcgtggtggg cactgctatt cttaatcccg
    10621 tttatcggct ggcttattat catcgtcttt aactgccagg cgggtacgcc aggcgaaaac
    10681 cgctttggac cagatccaaa actggaacaa gaataatgat taagcacctt gtaaaaggtg
    10741 cttcaaattg ttgacaaagt gcgctttgtt tatgccggat gcggcgtaaa cgccttatcc
    10801 agcctacaaa ttgccaaaat tcaatatatt gcagtgactg cgtaggcctg ataagcgtag
    10861 cgcatcaggc agttttgcgt ttgtcagcag tcagaagcac cttgtgaaag gtgcttaata
    10921 gattattttc cagcgaacaa ctttggaatt tcccgcagac accaggattt cgcttccccc
    10981 atactgtcac gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg
    11041 acacgcaacc gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg
    11101 ccaagccccg ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc
    11161 tgtttgtcca gcagctgtac ggtcaacacc gggcgctcac gagcggtatc cgctaccgca
    11221 attccacgat atttcacacg cgtcacttca gataacggtt ccggctcctg atgaatcggg
    11281 tgatcaggcg cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc
    11341 gacgaggaac gaaaatgcat atccggcgcg ataacgatat ccgcccgccc ctgctccagc
    11401 cgttcccacg cccccgccag cacttctgtg atgattgcca gttgggtatt ggcttttgcc
    11461 gccagtttgt cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc ggtcacaatg
    11521 gtgagatgcg tttcccaacc gcgcgcgaga gcttccgcat cagtagtcag tttatctgcg
    11581 gcttccagca aaacgcgccc tcgctccagc aacatcctcc cgacattggt gaatttggtg
    11641 cgatggcccg agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatgatg
    11701 tagctaagtg cggaaggcac gcgtcccagc tcatccgctg ccgccgcaaa actgccccgg
    11761 cgatcgatcg catccataac ccgtagtgct tccagcgtta atgccctttc tttggccatt
    11821 tcgttctcat tcaggaaatt tgaacatacc gggcagaata tctggctaac aatgtagcgt
    11881 ccagcccctt accataaaag gaagtaaaga gaggtcaaga attatgatta ctacccgaac
    11941 tgccaggcag tgtggacaag cagactacgg atggttgcag gcccggtata ctttttcctt
    12001 tggacactac ttcgacccga aattgttagg ctatgcctcc ctgcgtgtgc ttaaccagga
    12061 agtgctggcc ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg atattttaaa
    12121 tgtgattctg gatggagaag cagagtatcg cgacagcgaa ggcaatcatg ttcaggccag
    12181 cgccggagag gcgttgctgc tctctaccca gccgggtgtc agctatagcg aacacaatct
    12241 cagcaaagac aaaccgttaa cgcgaatgca gctttggctg gatacctgcc cgcagcgaga
    12301 gaatccgctg attcaaaagc tggcgcttaa tatggacaag cagcaattaa tcgcctcgcc
    12361 agagggtgcg atgggaagcc tgcaattacg ccaacaagtg tggctgcacc atatcgtgct
    12421 cgacaaaggc gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt tgcaatcgat
    12481 tcacgggaaa tttcatgcgc ttacgcatca tgaagagaaa gcggcgctaa cctgcggtga
    12541 tggggcgttt attcgtgacg aggctaacat tacgctggtt gccgattccc cactgcgcgc
    12601 tttgctgata gatttgcctg tctagttgtt tttacaggga gatgatgatg agtaaaaaat
    12661 cgaccaaaaa gcgccagcta gcgaagcccg tggtggcgaa agaacctgct cgcaccgcca
    12721 aaaattttgg ctatgaagag atgttgagcg agctggaagc tatcgtcgcg gatgctgaaa
    12781 cgcgtttagc cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc ggcgtaaacg
    12841 ccttatccgg cctacaaaac acaacaacat caacaaatta aacaccccgt aggccggata
    12901 agatgcgcca gcatcgcatc cggcatcagg tacaaaacac aacaacatca acaagttgag
    12961 caccccgtag gccggataag gtgcgccagc atcgcatccg gcacaatact caaaacctgg
    13021 cgtttatctg gccttgctcg ccataatctc gataatctgc cgatccgttt gctgcatcga
    13081 atggcttgct aacgcacaca ggttggcaat cgactgctca acatcatgcg ccacgatccc
    13141 ttcattgccg gtcacggcgg tatcatccag cgccattaac accgctttcc acgcagccga
    13201 agcactggtc gaaaccttcg caccatcgca aatcatgccg ctgacatcgc cgatcatact
    13261 gctgatcgcc atcgagatag tttcataacg cccatccacc agccatgcca tcccggcggc
    13321 ggcccccatt gctgcggtcg ttgcggcaca aagtgcagac aaacgcggta actggttatg
    13381 gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc gccagtcgtt catcatccgc
    13441 tccgaagtgt tctgctacaa ccaccacagg cattgttgcg gtgatcccct ggttacccga
    13501 gccggagtta ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct
    13561 ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgct cgcactgttt
    13621 ttccagcgtc gcgccaatat gcagccccca cttaccgctc aaaccttcct gcgataacgc
    13681 acaatttagc ttcgcggaat cgagaataaa gcggatcgcc gcaaatggga cttcattgac
    13741 gaacttcagg atctcagcca gcgtcgttct ggaaagcacc gtcagcggcg actcttgctc
    13801 gccctctgcc acacacgcct gctgggtaaa caccacacca ttgtgcgtct caatatgcac
    13861 aatgttggta tgcccgccga cgatggtgac acacgcccac ttctcaccgt tccagacttt
    13921 ggcgcgtgag aagaggattt cattgcaagg ttcctggatc ttaacagaga ctttccccgc
    13981 cgccagcagt gctttggcat cggcaattgc ctgcgcagtt gcgtctttca gcacttccag
    14041 cccggcgttg gcatttccac ctaacgcccc cagcgccgcc gcaatcggca gccccaccat
    14101 tcccgtgccg ggaacggtga tgcccagacc gttcttcatc agatttggcg aaacccaggc
    14161 ttctacacgt tcaaccggac cttccagttc tgccgcagca accgccgccg ccagcgccag
    14221 tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta
    14281 acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa aatttcaggt
    14341 aaatcaagaa aatgccagga acagagaaac acaaagcaac aaaccagtga cgataatcag
    14401 gtacagagac atccctttgt atttgtgcaa tgccggtact ttgtaaacca gccacgccgg
    14461 gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg
    14521 tgcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga tgccgcgctg
    14581 aacaagattt tcgttaatct tctcggcagg catcttgcga cgcaggatgt tcattacgat
    14641 cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca tgacagcaaa
    14701 gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc taataaactg
    14761 tgcggcaatc gccagcgcgg aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc
    14821 catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc
    14881 gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt cccgcgagcg
    14941 ataagagatc accatcgggc ttaacgtctg gataaacaga atcgacgtca gggtaaacgg
    15001 cagcgtaata atggcgtttt tcaccagcag ccccagcggc ggtagcgaac cgacgttata
    15061 cagatgccac attcccacca tcgacacgcc cagcgcagcg actaccagca gcttggtcag
    15121 caccatgtcg gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga tcgccaccag
    15181 aatgcaaatc agtaccagac cataaaaggg actgtctgac agcaaccctt ccgtcacgcc
    15241 gaaggtatgc agataggaag cactatcgtt ggtgatggcg gtggaataga cgaacatcca
    15301 aatcaccagc attacgaaat agagcgcacc taacaggatg ccccagtttt tacctaaata
    15361 accgctaatg acactcgggt aatctttaca ttctggtgat tctgccagcg tattaataaa
    15421 caaccgctga aacagataca ttgccgggta accaatcacc gatgagagca aaaataccca
    15481 caatcccatc aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc
    15541 gatactcata atcacccagc aggtgtcagt actgtcgaat ttgatcgctt ctcgccactc
    15601 gctctcactc attccagcac gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt
    15661 cgatgcaatt tccataattt ctcgctcaat attttgtagg gcttattatt tttttccgag
    15721 ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga gttattagaa taaagaaatg
    15781 atacgcgcga ggtcagagaa aatcttcaca atcaagactt taaaataaca gagatggata
    15841 agaattttct actaaattaa tcgcaagaga atattcattc tctatttgcg gcagatcaca
    15901 aaaaaaaggt gcac
//
LOCUS       DAJTYF010000090        15886 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTYF010000090 DAJTYF010000000
VERSION     DAJTYF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.090"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..378
                     /gene="tuf"
                     /locus_tag="OO590_002922"
     CDS             <1..378
                     /gene="tuf"
                     /locus_tag="OO590_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6638220.1"
                     /translation="RAGENVGILLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILS
                     KDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDD
                     GLRFAIREGGRTVGAGVVAKVLG"
     gene            561..755
                     /gene="bfd"
                     /locus_tag="OO590_002923"
     CDS             561..755
                     /gene="bfd"
                     /locus_tag="OO590_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6638221.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            828..1304
                     /gene="bfr"
                     /locus_tag="OO590_002924"
     CDS             828..1304
                     /gene="bfr"
                     /locus_tag="OO590_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6638222.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1301..1768)
                     /locus_tag="OO590_002925"
     CDS             complement(1301..1768)
                     /locus_tag="OO590_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6638223.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            2147..2458
                     /gene="rpsJ"
                     /locus_tag="OO590_002926"
     CDS             2147..2458
                     /gene="rpsJ"
                     /locus_tag="OO590_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6638224.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2491..3120
                     /gene="rplC"
                     /locus_tag="OO590_002927"
     CDS             2491..3120
                     /gene="rplC"
                     /locus_tag="OO590_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6638225.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3131..3736
                     /gene="rplD"
                     /locus_tag="OO590_002928"
     CDS             3131..3736
                     /gene="rplD"
                     /locus_tag="OO590_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6638226.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3733..4035
                     /gene="rplW"
                     /locus_tag="OO590_002929"
     CDS             3733..4035
                     /gene="rplW"
                     /locus_tag="OO590_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6638227.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4053..4874
                     /gene="rplB"
                     /locus_tag="OO590_002930"
     CDS             4053..4874
                     /gene="rplB"
                     /locus_tag="OO590_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6638228.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4891..5169
                     /gene="rpsS"
                     /locus_tag="OO590_002931"
     CDS             4891..5169
                     /gene="rpsS"
                     /locus_tag="OO590_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6638229.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5184..5516
                     /gene="rplV"
                     /locus_tag="OO590_002932"
     CDS             5184..5516
                     /gene="rplV"
                     /locus_tag="OO590_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6638230.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5534..6235
                     /gene="rpsC"
                     /locus_tag="OO590_002933"
     CDS             5534..6235
                     /gene="rpsC"
                     /locus_tag="OO590_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6638231.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6248..6658
                     /gene="rplP"
                     /locus_tag="OO590_002934"
     CDS             6248..6658
                     /gene="rplP"
                     /locus_tag="OO590_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6638232.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6658..6849
                     /gene="rpmC"
                     /locus_tag="OO590_002935"
     CDS             6658..6849
                     /gene="rpmC"
                     /locus_tag="OO590_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6638233.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6849..7103
                     /gene="rpsQ"
                     /locus_tag="OO590_002936"
     CDS             6849..7103
                     /gene="rpsQ"
                     /locus_tag="OO590_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6638234.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7268..7639
                     /gene="rplN"
                     /locus_tag="OO590_002937"
     CDS             7268..7639
                     /gene="rplN"
                     /locus_tag="OO590_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6638235.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7650..7964
                     /gene="rplX"
                     /locus_tag="OO590_002938"
     CDS             7650..7964
                     /gene="rplX"
                     /locus_tag="OO590_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6638236.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7979..8518
                     /gene="rplE"
                     /locus_tag="OO590_002939"
     CDS             7979..8518
                     /gene="rplE"
                     /locus_tag="OO590_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6638237.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8533..8838
                     /gene="rpsN"
                     /locus_tag="OO590_002940"
     CDS             8533..8838
                     /gene="rpsN"
                     /locus_tag="OO590_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6638238.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8872..9264
                     /gene="rpsH"
                     /locus_tag="OO590_002941"
     CDS             8872..9264
                     /gene="rpsH"
                     /locus_tag="OO590_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6638239.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9277..9810
                     /gene="rplF"
                     /locus_tag="OO590_002942"
     CDS             9277..9810
                     /gene="rplF"
                     /locus_tag="OO590_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6638240.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9820..10173
                     /gene="rplR"
                     /locus_tag="OO590_002943"
     CDS             9820..10173
                     /gene="rplR"
                     /locus_tag="OO590_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6638241.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10188..10691
                     /gene="rpsE"
                     /locus_tag="OO590_002944"
     CDS             10188..10691
                     /gene="rpsE"
                     /locus_tag="OO590_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6638242.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10695..10874
                     /gene="rpmD"
                     /locus_tag="OO590_002945"
     CDS             10695..10874
                     /gene="rpmD"
                     /locus_tag="OO590_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6638243.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10878..11312
                     /gene="rplO"
                     /locus_tag="OO590_002946"
     CDS             10878..11312
                     /gene="rplO"
                     /locus_tag="OO590_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6638244.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11320..12651
                     /gene="secY"
                     /locus_tag="OO590_002947"
     CDS             11320..12651
                     /gene="secY"
                     /locus_tag="OO590_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6638245.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12683..12799
                     /gene="rpmJ"
                     /locus_tag="OO590_002948"
     CDS             12683..12799
                     /gene="rpmJ"
                     /locus_tag="OO590_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6638246.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12946..13302
                     /gene="rpsM"
                     /locus_tag="OO590_002949"
     CDS             12946..13302
                     /gene="rpsM"
                     /locus_tag="OO590_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6638247.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13319..13708
                     /gene="rpsK"
                     /locus_tag="OO590_002950"
     CDS             13319..13708
                     /gene="rpsK"
                     /locus_tag="OO590_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6638248.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13742..14362
                     /gene="rpsD"
                     /locus_tag="OO590_002951"
     CDS             13742..14362
                     /gene="rpsD"
                     /locus_tag="OO590_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6638249.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14388..15377
                     /gene="rpoA"
                     /locus_tag="OO590_002952"
     CDS             14388..15377
                     /gene="rpoA"
                     /locus_tag="OO590_002952"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6638250.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15418..15801
                     /gene="rplQ"
                     /locus_tag="OO590_002953"
     CDS             15418..15801
                     /gene="rplQ"
                     /locus_tag="OO590_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6638251.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4087 a         3740 c         4234 g         3825 t
ORIGIN      
        1 cgtgctggtg agaacgtagg tattctgctg cgtggtatca aacgtgaaga aatcgaacgt
       61 ggtcaggtac tggctaagcc gggcaccatc aagccgcaca ccaagttcga atctgaagtg
      121 tacattctgt ccaaagatga aggcggccgt catactccgt tcttcaaagg ctaccgtccg
      181 cagttctact tccgtactac tgacgtgact ggtaccatcg aactgccgga aggcgtagag
      241 atggtaatgc cgggcgacaa catcaaaatg gttgttaccc taatccaccc gatcgcgatg
      301 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgtt
      361 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
      421 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      481 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      541 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      601 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      661 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      721 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      781 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      841 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      901 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      961 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
     1021 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
     1081 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
     1141 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
     1201 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
     1261 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
     1321 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1381 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1441 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ccaggcgcca agggcggcga
     1501 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1561 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1621 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1681 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1741 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1801 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1861 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1921 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1981 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     2041 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     2101 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     2161 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     2221 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     2281 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     2341 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2401 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2461 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2521 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2581 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2641 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2701 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2761 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2821 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2881 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2941 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     3001 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     3061 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     3121 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     3181 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     3241 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     3301 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3361 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3421 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3481 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3541 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3601 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3661 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3721 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3781 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3841 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3901 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3961 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     4021 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     4081 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     4141 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     4201 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     4261 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     4321 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4381 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4441 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4501 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4561 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4621 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4681 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac tgcgatgaac ccggtagacc
     4741 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4801 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4861 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4921 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4981 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     5041 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     5101 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     5161 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     5221 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     5281 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     5341 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5401 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5461 cagatcgtat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5521 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5581 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5641 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5701 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5761 tatcggtaag aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5821 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5881 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5941 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     6001 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     6061 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     6121 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     6181 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     6241 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     6301 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6361 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6421 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6481 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6541 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6601 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6661 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6721 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6781 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6841 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6901 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6961 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     7021 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     7081 ttgtagagaa agcggttctg taatacagta cactctctcg atatgaataa acggctcaga
     7141 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     7201 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     7261 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     7321 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7381 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7441 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7501 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7561 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7621 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7681 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7741 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7801 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7861 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7921 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7981 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     8041 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     8101 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     8161 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     8221 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     8281 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     8341 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8401 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8461 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8521 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8581 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8641 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8701 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8761 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8821 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8881 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8941 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     9001 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     9061 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     9121 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     9181 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     9241 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     9301 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9361 aaaaacggcg agctgacccg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9421 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9481 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9541 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9601 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9661 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9721 cgcgcctacc gtcgtcctga gccttacaaa ggcaagggtg ttcgttacgc cgacgaagtc
     9781 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9841 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9901 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9961 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
    10021 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
    10081 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
    10141 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
    10201 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
    10261 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
    10321 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10381 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10441 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10501 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10561 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10621 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10681 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10741 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10801 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10861 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10921 cgggtaaacg cctggggcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10981 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    11041 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    11101 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    11161 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    11221 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    11281 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    11341 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11401 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11461 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11521 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11581 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11641 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11701 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11761 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11821 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11881 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11941 gagcaagcgc gtcaaggtga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    12001 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    12061 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    12121 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    12181 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    12241 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    12301 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12361 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12421 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12481 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12541 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12601 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12661 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12721 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12781 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12841 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12901 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12961 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    13021 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    13081 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    13141 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    13201 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    13261 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    13321 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13381 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13441 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13501 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13561 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13621 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13681 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13741 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13801 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13861 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13921 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13981 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    14041 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    14101 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    14161 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    14221 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    14281 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    14341 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14401 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14461 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14521 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14581 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14641 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14701 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14761 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14821 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14881 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14941 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    15001 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    15061 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    15121 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    15181 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    15241 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    15301 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15361 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15421 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15481 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15541 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15601 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15661 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15721 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15781 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15841 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DAJTYF010000091        15843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTYF010000091 DAJTYF010000000
VERSION     DAJTYF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.091"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..587
                     /locus_tag="OO590_002954"
     CDS             <1..587
                     /locus_tag="OO590_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6638252.1"
                     /translation="SSSWGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWK
                     ALIAGGSERGLIYTQFVAQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWL
                     QSRVYARAHHYYHSWMHYFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFE
                     ELAAGSDRFYATLPWQPLTVQPECPVTLKDVSDL"
     gene            584..2035
                     /gene="djlC"
                     /locus_tag="OO590_002955"
     CDS             584..2035
                     /gene="djlC"
                     /locus_tag="OO590_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6638253.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2095..3765)
                     /gene="hscC"
                     /locus_tag="OO590_002956"
     CDS             complement(2095..3765)
                     /gene="hscC"
                     /locus_tag="OO590_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR6638254.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(3849..4784)
                     /gene="rihA"
                     /locus_tag="OO590_002957"
     CDS             complement(3849..4784)
                     /gene="rihA"
                     /locus_tag="OO590_002957"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6638255.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            complement(4902..5627)
                     /gene="gltL"
                     /locus_tag="OO590_002958"
     CDS             complement(4902..5627)
                     /gene="gltL"
                     /locus_tag="OO590_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6638256.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5627..6301)
                     /gene="gltK"
                     /locus_tag="OO590_002959"
     CDS             complement(5627..6301)
                     /gene="gltK"
                     /locus_tag="OO590_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6638257.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6301..7041)
                     /gene="gltJ"
                     /locus_tag="OO590_002960"
                     /pseudo
     CDS             complement(6301..7041)
                     /gene="gltJ"
                     /locus_tag="OO590_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015698156.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
     gene            complement(7211..8118)
                     /gene="gltI"
                     /locus_tag="OO590_002961"
                     /pseudo
     CDS             complement(7211..8118)
                     /gene="gltI"
                     /locus_tag="OO590_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            complement(8515..10053)
                     /gene="lnt"
                     /locus_tag="OO590_002962"
     CDS             complement(8515..10053)
                     /gene="lnt"
                     /locus_tag="OO590_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6638258.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(10078..10956)
                     /gene="corC"
                     /locus_tag="OO590_002963"
     CDS             complement(10078..10956)
                     /gene="corC"
                     /locus_tag="OO590_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6638259.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(11046..11513)
                     /gene="ybeY"
                     /locus_tag="OO590_002964"
     CDS             complement(11046..11513)
                     /gene="ybeY"
                     /locus_tag="OO590_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6638260.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11510..12550)
                     /locus_tag="OO590_002965"
     CDS             complement(11510..12550)
                     /locus_tag="OO590_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6638261.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAREEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(12703..14127)
                     /gene="miaB"
                     /locus_tag="OO590_002966"
     CDS             complement(12703..14127)
                     /gene="miaB"
                     /locus_tag="OO590_002966"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6638262.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14273..15448
                     /gene="ubiF"
                     /locus_tag="OO590_002967"
     CDS             14273..15448
                     /gene="ubiF"
                     /locus_tag="OO590_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6638263.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(15602..15676)
                     /locus_tag="OO590_002968"
     tRNA            complement(15602..15676)
                     /locus_tag="OO590_002968"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15642..15644),aa:Gln,seq:ctg)
     gene            complement(15714..15788)
                     /locus_tag="OO590_002969"
     tRNA            complement(15714..15788)
                     /locus_tag="OO590_002969"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15754..15756),aa:Gln,seq:ctg)
BASE COUNT         3835 a         4049 c         4127 g         3832 t
ORIGIN      
        1 tctcatcatc ctggggaata aataatcgtg atgatttaat tcagactatt tatcagatga
       61 ctgatgatgg tcatgccaat gatttagctg gcttatatct gacatggcaa cgctcttccc
      121 ctgaagagtg gaaagcatta attgctggtg gctcggagag agggttgatt tatacccaat
      181 tcgttgccca aaccgctatg tgctgtggag aagggggaat caaagcctgg gattatgttc
      241 gaatggggtt tctgtcacgg gccggtgtgc tcaacaactg gctgacggaa gaagagagtt
      301 tatggctgca atcgcgcgtc tatgcacgag ctcaccatta ctatcacagc tggatgcact
      361 atttttctgc ctattcacta gggcgtctct actggcaatc ttctcagtgc gaagacaacg
      421 cttcgctacg cgaggcgcta accctgtaca aatacgatag cgccgggagt cggatgttcg
      481 aagagctggc agcgggaagc gaccgtttct atgccacgct tccctggcag ccattaactg
      541 tccagcctga atgcccggta acgcttaagg atgtgagcga cctatgaaaa catgttggca
      601 aatcctcgaa attgaaagca cgacgcaaat agacattatc cgccaggctt atcttgctcg
      661 cttaccgttg tgtcatcccg aaaccgatcc gcaggggttt aaagcattac gccaggccta
      721 tgaagaggcg ctgcgactgg cggtaaatcc tgtcgaggaa gcagatgatg aagaaaaaga
      781 tgccaccgct gaacatgaaa tactacgtgc attcaggaca ttactggatt cagaaagtga
      841 tcgttttcag ccctccgcct ggcagaaatt tattcagcaa ttaaatacct ggaacatgga
      901 ggatgtcgat caattacgct ggccgctgtg tgcaatcgct atagaagcgc gatatctgtc
      961 attaaattgt gcttctttgt tggcagagcg tttgaactgg cattcgttta atgacagcga
     1021 aggaatggat gaggaagaaa gggaggcttt tcttgaggcc attcaggctg gtgattgttt
     1081 cgatttcctt agccttctgg aatatcccgt cgcgttacag aaccagactg ttgaatatta
     1141 cttcgcgctg gaacgttgct gccgttacca tcctgactat gtcactgcgt ttttggcgat
     1201 ggaaggaccg tggttcattc ctgatgatgc aaagttacat cgcaaactgt tgcgctggta
     1261 cagctcggtg caaacaggta tggtggaact cattcctgta gccaaacagt ggcaagcgga
     1321 agaaccagaa agcgaagatg cccggtatta ccagtgtgca caacgtttgt actgcggcga
     1381 gggggaaagc ctgcttgctg atctcggcgc gtactgggaa agttacccat ctacacaagc
     1441 tgataatttg ttgttgcagt ggagcaagcg tcattgcccg gattatttcg cgttattagt
     1501 gatggttatc gaagcgcgga gcatggtaga tgcgcaaggt caaccgctga aatatgttcc
     1561 tggtgagagc gcccggacgc ggctgttatg ggcggaaatt ttacatagcg gaaaattatc
     1621 gccgttaggt caatcgttta ttgagtcgtt attcttcaaa cgcaaagcat gggcgtggtg
     1681 gaaatcgaga gtcggtagcg agacagaaga agattcaccg ttactggatt tgtatcgggt
     1741 agcggaacag gtagtacttg aagcgtttcc gaaacaagag atgctggccc gtcttaatac
     1801 aaggctggaa ggcggagatg ctcatccatt agaggccatt atcacccgga tgctgttgac
     1861 gaaagtaaaa ctcgagccgg aggatgaaga tgtcgatgag ccaacacctg aaaaccatga
     1921 agaaaaaaat gatgagggtg aaaaaccaca gagcattacc agcattatca aaatcagttt
     1981 aacggtgctg gttataggtt atgctctcgg caaaatcgcg atgttgttta gttgacgatg
     2041 tgttttttgc gccgatgccg gatgcagcat ttacgccgca tccggcagcc gtgattatga
     2101 ggtctcaatg gctaaataaa cgctcgcccg ccgcctgacc tcatcgacgc gggccatatc
     2161 gtttgactgc agcgccgcat caaaatcggt gatcacccgg ccaatctctt ctcgctcgtc
     2221 acccagcgcc cgcgcccaca actcttccag tttggcttta aaggtgcgat tgatgagcat
     2281 atcgcgcgga taaattttca atgcggataa ccgcgtccga ctctcttcaa tctgctgcgc
     2341 gctcaatgtt accgggctgt ggttaatcac tctggactta acgctgccgt cttccagaag
     2401 cacgtcaact tcaagcaacc cgttgatatc ataactaaag cgaatatcaa tcgactgata
     2461 agccccggtt ttcttcaacg gcacatcgaa ggattccacc agaatgttgt ttttaacttt
     2521 gtggttttct ccctgataga cgttaaccgt aattgaatcc tgttccgggt gcatggttga
     2581 ataagtttct acacgcgaca cgggcacagt ggtgtttcgt tcaataatcg gcgagaaaat
     2641 gccggaaacg ccctggcggt taacctcaac gcccaacgag taagggcaaa tatcagtgag
     2701 gatcacttct tcaatatctt cactgcgtaa gcggcaggcg gcctggattg ctgcgcccag
     2761 cgcgacaatg gtgctcggat cgtaactttg atacggtaat ttgccaaaca gacgcacggc
     2821 gattcgctgc accagcggca tttgtgacgc gccgccaacc agcaccagac tgtcgatttg
     2881 actcggcttc agacgcgcat cgcgcaacgc ctgttcaatc ggcactcgca agcgattgag
     2941 cagcggcaac cacaaatctt ccagttcgtt ctcgtaaaat tcgcattccc gcgtttcatc
     3001 ctggtactgc cagcgaatgt gcattggcga ttgattgcta cattttgccg cttccacaca
     3061 ggcgtacaag gctgccagtt cactctcgtt aagcgtggtc cttgtgacat ccgcgcgctt
     3121 taaaacgtca tcgaccaaca tatgggtaaa atcttcgcca ccgaggaagt tgtcgccagc
     3181 ggaggcgtga acttcaatca ccggcgtggc gtactcaagc accgtaacgt caaacgtgcc
     3241 gccaccgaga tcaaacacca gcgaacgggt attttgttgg gtatgcaggc catacgccat
     3301 cgcagctgct gtgggttcat taattaagcg taccgcattt aacccggcta agtccgctgc
     3361 taaacgggta tgcttgcgtt gtttatcgct gaaataagcc ggaacggaga tcaccacatc
     3421 tttaatcgga cgttgcagaa attcttcggc atcttctttt aatgagcgta ataccaaaga
     3481 ggacagttct ggcgcgttaa atgtgtcgct gcctaaccgc cagttggtat tactgcccat
     3541 tgcacgttta aataacgctg ccgttttatc cggatgcgaa gtacgccgtg atacagccgg
     3601 ttttccgact aaaatatgat tattttcatc catgctaatt atggatggtg ttaaatattc
     3661 accgaactta tttggaatta attgcgcggc accgtctttc cagacggcaa ttaaactatt
     3721 ggtagtgccg agatcgatac ctatggcgag ttctgcatta tccattgcat ttatatcttg
     3781 aaagaaaaag agataaatag attaagacca cccaggtaac gcgtggtgat cttaatcaat
     3841 gacgagtgtt aagcgtaaaa tttcagacga tcagccagta aatcaacaaa gccctgacga
     3901 tcaacatcga caattacagt ggcattcggt ttattgccag tcagataata ataatcaaca
     3961 accgtcatac ccagggtata tttcccctgt gtttccacgc caacccagcg ctcaacagtg
     4021 gtaaacaact cgggtttcaa cagccaggcg atggtgcatg ggtcatgcag cggtgcgccg
     4081 acaaagcccc atttttcgtc tttatgatat tcgaggaaga aatccagcag ttcggcaaca
     4141 atggttgaaa cagggttgcc aatcgcgcgg aaacgctcgg tgtcttcaac gtggatttgc
     4201 gctttatgag taacatccag accggccatc accaccggga tccctgactg gaagacaatt
     4261 tctgccgctt ccgggtcaac gtaaatatta aattcagccg caggcgtcca gttaccaagc
     4321 cccattgcgc cgcccataat cacgatacgg gcaattttgc tatgcagttc cggatggcta
     4381 ttgagcagca aggcaacgtt agtttgcggt ccggtagaaa caatcgtgac aggttccgca
     4441 ctttcgcgca gcgttttcgc catcagctcc accgccgtac agttttgcgg tgcgaacgtc
     4501 ggttccggta atgccgggcc gtcgagaccg ctttcgccgt gcacattgtc cgcgataatc
     4561 aactcacgca ttaacggttt taccgcgccg cctgccaccg gaatatcggt gcgattaagc
     4621 aaggtcagca tacgcagaac attgcgtaag gttttttctg gtgtctggtt tccggcggaa
     4681 gacgtaattg ctttgacatc aagctctggt gaggcgaggg cgagaactat tgcgatagcg
     4741 tcatcatgac ctgggtcgca atctaacaga attggcagtg ccattgttgc tccttgttgt
     4801 gtgcttcttt gcgacaaggg taacgccagg atgtaacaga tacgaggggc gaaacgataa
     4861 agcgtgagat ggcgcgcaat tgggtatgcg cgccagagtg attaatgcag gattttcgcg
     4921 aggaagtctt ttgcgcggtc cgatttcgga tcatcgaaga aagcgtcttt cggcgagtct
     4981 tcgacaattt taccctcgtc cataaagatc acccgattcg ccactttgcg ggcaaagccc
     5041 atttcgtggg tcaccaccat catggtcatt ccttcgttcg ccagttccac catcacgtcc
     5101 agcacttcgt tgatcatctc cggatccagc gccgatgtcg gttcgtcgaa cagcatcgca
     5161 ataggagcca tacacaatgc gcgagcgatt gccacacgct gctgctgacc gccggaaagc
     5221 tgcgccggaa atttattggc atgtgcggaa agtcccacgc gctccagcaa tttcaatgct
     5281 ttttcacgag cgggcgcttt atcgcgttta agcactttca cctgcgccag ggtcaggttt
     5341 tcgataatcg acagatgagg gaacagctcg aaatgctgga ataccatccc gacgcgggag
     5401 cgcagctttg ccagatcggt tttcttgtcg ttaaccacga taccatcgac ggtgatttca
     5461 ccttgctgca ctggttcgag gccgttgacg gttttaatca gcgttgattt gccggaacca
     5521 gacgggccgc aaaccaccac cacttcgcct tttttcactt cggttgagca gtcggtcagc
     5581 acctgaaagt gaccatacca ttttgaaaca tttttcaggg taatcattat gctgtccttc
     5641 ttttcaagta gctgaccaac aacgacgcgc taaggctaat aacgaaataa acaaatccgg
     5701 caaacaggat catctcgacc tgcgtaccat cacgctcacc aatggttgag gcggtacgga
     5761 agaaatcggc cagagataac acatacacca gcgaggtatc ctggaacagt acgatgccct
     5821 gagtgagcag cagcggcacc atcgcgcgga aagcctgcgg cagaataatc agtttcatcg
     5881 actgccagtg agtcatcccc aacgccagcg cggcgctcga ctgaccacgg gaaatacttt
     5941 gaataccggc acggataatc tctgaatagt aggccgcttc aaacatcgaa aacgccacca
     6001 tcgccgaaat taaacgaata tcattttttg gcgataatcc cagcacattt tgcagaaatc
     6061 ccggcacaat caagtaaaac cacagcaaaa ccatgactaa aggaatcgag cggaatacgt
     6121 taacgtaggc tttggcaaac cacgaaacag gcgcgaagct ggacaagcgc atcaccgcca
     6181 gcatggtacc ccacaaaata ccaatcacga ctgccgtgac ggtgattttc agggtgatca
     6241 ccagcccgtc gagcagatat ggcagggaag gaacaatgga actccagtca aactcgtaca
     6301 ttatttgccc cccatgttgc cgggcaggcg aactttacgt tcaaccagcg tcatcaccag
     6361 catgataaaa gcgttaatca acacatacgt aagcgtgatg gcggtaaacg actcccaggc
     6421 gtgagccgag tagtcgagca atttacccgc ctgcgccgcc atatccacca gaccgatggt
     6481 cgaggcgatg gcggagtttt tcaccaggtt catcatctcg gaagtcatcg gcgggacgat
     6541 aacgcgataa gcattaggca gcagtacgta tcgataagtc tgcggtagcg tcaggcccat
     6601 cgccagcgcg gcattcttct gcccacgcgg tagcgactga atagcggcgc gtacctgttc
     6661 gcaaacacgg gaggcggtaa acagccccag gcagagcatg gatgaaagga aaaactgaat
     6721 attgggatcc agctcggcct taaaccacat gccgattttc tccggcagca gctccgggat
     6781 caccagatac caggtaaaga attgcacaat caacggcacg ttgcggaaca gttcgacata
     6841 caacgtaccc agaccggaga gaaaacggtt tggaacggta cgtaaaatgc cgaaaaatga
     6901 accgacgagg aaagcgataa ttcaggcgca gatcgacaaa gcgatcgtca cctgaaaacc
     6961 actccagacc caaccaagat aggtggtatt gccgaacggg gcttgttgta aaaaaatacc
     7021 ccagttccag tctatagaca taaatctact ccagaaaaaa gagggtagca gcgttaactg
     7081 ctaccctcga agattgttac ccagcgtatt gcggttttca ggcccgatgg ggaacgacca
     7141 tcaggcgtat agtctgtccg tgctacgtaa caatcgagag ggctggaatt tccgcctctg
     7201 gttcttgtaa ttagttcagt gccttgtcat tcggttcttt gaacagtgct ttcatttcgt
     7261 ctgacagttc gaaattcatg ttcaggtttt tcggcggaat tggatttttg aaccatttat
     7321 caaaccattt ttccgcttca ccggaggtct gcacctgagc gatagtgtca tccatcagct
     7381 ttttgaactg cggatcgtct ttacgcaaca tgcaaccgta ggcttcctga gactgcggct
     7441 tgccgacgat atcccagttg tctggtttct tcgctttcgc acgttcaccg gccagcagag
     7501 cgtcatccat cataaaggca acggcacgac cgctttccag ggtgcggaaa gagtcaccgt
     7561 gatctttggc gctgatgatg cgcatattca ttttttgctc ttcattcagt ttgttgagca
     7621 aaacttcaga ggtagtgccg gaagtgacga ctacggcttt gcctttcagg tcggcaaaat
     7681 ctttgatatc gccgcccttt ttggtcaaca ggcgcgtacc gaccacgaaa atagtgtcag
     7741 agaaagccgc ctatttttgg cgttcgacgt tgttggtggt agaaccacat tcaaaatcga
     7801 aagtgccgtt ttgcagcagt ggaatacggt ttgtgaggta atcggaatca gttttacctg
     7861 caagtccggt ttgttgagtt tctttttcac tgcttcaaca atggcgttgg agtaatcctg
     7921 cgagtaaccc accacttttt gctgattgtc gtaataagag aaaggtactg aagattcacg
     7981 gtgaccgacg acaatcacac cgtttttggc gattttgtcc agcgtactgc ctgctgccgg
     8041 ggcggcgtca tctgcctgtg ccagtcctgc ggaaagcgcc agggcgagga ttgctgtggc
     8101 aggtttacgt aattgcatat ccaactcctt tatcttctgc gttaagaacg catggatacc
     8161 cgttgtgagt gtttgtgttg ttatcgtctg caactttatt gtgcagtgtt gtgcctgtta
     8221 ggtaacattt agtttggcta aatgtaaaga tattgatgtt ttattgtttg tttttgcgag
     8281 gtgcaccgca ccattctgaa gcaaaattct taaaatgcac tcttttggtg ctaccggtgg
     8341 attgctgtgg tgcaaccggg ttgtactgat gctggttcaa gctttgcctt gtataacaaa
     8401 gcaatagatg tgccaaagtt ggataggaga atattgttat ccggataatg cactgatgcc
     8461 gcatccggtg agcgtgccga aatatgggat gtattccggc acgataagaa gggattattt
     8521 acgtcgctga cgcagactca tcaacacagc agcaaaacca aacagtgccg tcagcaccca
     8581 cagcggccag ttgccggtac gtgcgtatgg tgtgagtccg gtggtcggcg tcacgttagt
     8641 ggttaacacc tcgcgggtga actgcgggat catcgcctga atctcaccct gcgggccaat
     8701 caccgccgta atgccgttgt tggtgctgcg caacagtggg cgcgccagct ccagcgcacg
     8761 cattcgcgcc atctggaagt gttgccatgg accaatagat ttaccaaacc acgcatcgtt
     8821 ggagatagtc agcaggtagt cggtatccgg gcggaagtta tcgcgcactt gctcgccgag
     8881 aatgatctcg tagcaaatag ccgcagtaag ctcaatacca tttgccgaca gcggcggctg
     8941 gatatatggc ccacggctga acgacgacat cggcagatca aagaacggtg ctaacggacg
     9001 cagaatcgac tccagcggga caaactcgcc aaacggcacc agatggtttt tgttatagcg
     9061 atcggctgat tcgtagctgt acggtgcacc tttacccagc gtgatgatgg tgttgtaggt
     9121 atcgtagcgg ttctgcttat tgagacgcgc gtcgacaatc ccggttacca gcgagctacc
     9181 tttatcacgc aactcaccgt ccagtgcttt gaggaacggt tgctgattaa tttccagatc
     9241 ggttatcgcc gactccggcc agataatcaa cgatgatttg cccatcagcg gtgccgttgc
     9301 gttgtagtaa atctttagcg tattaagaag ctggccttcg tcccatttca gcgattgcgg
     9361 aatatcgccc tgaaccatcg aaacctgaat ggttttctcc ggttgcgggg taaaccattg
     9421 gatgtaacgc agcgggaagg gtagggcaaa cagcacgacg gccaccacca gcggacgcca
     9481 gttgcgtttg accaacgcca gtgccagcag gccactaacc atcatcagca ggaagttaat
     9541 ggcttccacg cccattagcg gtgccagccc ttttaacggg ccatcaatct ggctatagcc
     9601 gaactgtaac cacgggaagc cagtcagcac ccaaccgcgc agaaactcgg tcacttgcca
     9661 gagagcaggg gcggcaatcg ccacgcgcag ccaggtggtt ttcggccaca gacgcgacag
     9721 cacgccagca aacagtccgg tatacagcga caaatacgcc gccagcagca ccactaggaa
     9781 gatgttaacc gggccaggca tcccgccaaa ggtcgcgatg ctgacataga cccagttaat
     9841 accgctgcca aagaggccaa atccccagca aaagccaata gcggcagact ggagtgggcg
     9901 acggttaaag gtcaacgcct gaagccccat cagcgaaata atcgccgcag gccagacgtc
     9961 gtaaggagag aaggccagcg ttccgcaggc accgaataat aacgccagca gcaggcgaat
    10021 gcgctggcgt tcaattaatg aggcaaaagc catgtagtta tctatccagt ttcggtttta
    10081 ttcatccagc tttggctggg gtgagtcatc cgggattttg acatgaacct gaataatacg
    10141 ccgactatcg gccatcgcca ctttgaactg gtaaccgtcg atgtcgatag tttcgccacg
    10201 cgccggaaga tgcccaaacg cctgcatcac cagaccaccg atagtgtcga cttcttcatc
    10261 gctaaagtgg gtgccgaacg cttcgttgaa gtcttcaatg gaagccagtg cgcgcacggt
    10321 ccaggtatga cgactcagct gacggaagtc gatatcatct tcttcgtcat actcgtcttc
    10381 aatctcacca acaatcagtt ccaggatgtc ttcaatggtc accagaccgg aaaccccacc
    10441 gaattcgtca ataacgatcg ccatgtggta acgctgagag cgaaactctt tcagcatccg
    10501 gtctacgcgc ttactttcag gaacgacaac cgcctgacgt aacactttgt ccatgccgaa
    10561 ggcttcagca tcgctgcgca taaacggcag caagtctttc gccatcagaa tcccttcaat
    10621 gtgatctttg tcttcgctaa tcaccgggaa acgtgagtgg gcggactcga tgatgacatc
    10681 aaggcattcg tccagcgtct ggttgcgttt cagggtaatc atctgggagc gggggatcat
    10741 gatgtcgcga acgcgttggt ctgcgatgtc catcacccct tcgagcatat cgcgcgtatc
    10801 ttcgtcgata aggtcgttct gcccggaatc acggatcagc gccagcagtt cgtcacggtt
    10861 tttcggttca ccgtggaaaa gttggctgag taacagggag aaaaatccct tcttgttgct
    10921 tatcgtgtca ctactgtgtg aattgtcgtc gctcatggcg tcgtatgggt tctcatgtta
    10981 gttaatcaaa acgccgtcgt taatcaccaa cggcggggac gtctgccagt caaatgcctg
    11041 gcaaattatt ctttctcggc aatgtacgga tcctcatagc ccagagcaag cataatctct
    11101 gtttcgaggg cttccatttc ttctgcttcg tcatcttcaa tgtgatcgta acctaacaaa
    11161 tgcagactgc cgtgcaccac catatgcgcc cagtgcgcct ccagtggttt gccttgctcc
    11221 tgagcttcct tctcaaccac ctgacggcag ataaccagat cgcccagtag cgacatctcc
    11281 atgccaggcg gcacttcaaa cgggaaggag agcacgttgg tcggcttatc cttaccgcgg
    11341 taggtcagat tcagactgtg gctttcggcg gtatcgacca cgcgaatagt cacttccgac
    11401 tcttcctgaa actgcgggat caccgcattc agccatgttt gaaactggct ctcttccggt
    11461 aacccggaat tatcttcaca tgccagttgt aaatcgagga tcacctgact catttttgtt
    11521 cctgttcttc gcgcttgcgt tctgctgcca gcgccgcttt acgtttttgt tctgcttctt
    11581 ccctggcttc ataagcgtta acgatacgcg ccaccaccgg gtgacgaacc acgtcttcgc
    11641 tgtggaagaa gttaaagctg atctcttcga catcggccag cacttcaatg gcatgacgta
    11701 agcctgattt agtattacgc ggcaggtcga tctgtgtgac gtcgccggtg ataaccgctt
    11761 ttgagttaaa accgatacgg gtcaggaaca tcttcatctg ttcgatggta gtgttctggc
    11821 tctcatcgag aatgataaac gcgtcgttca gcgtacgacc acgcatatag gccagcggcg
    11881 cgacttcaat aacgttgcgc tcaatcagtt tctcgacttt ctcaaagccc agcatttcaa
    11941 acagcgcgtc gtacagtggg cgcagatacg ggtctacttt ctggcttaaa tcgccaggca
    12001 ggaagcccag tttctcaccg gcttcgaccg ccggacgagt cagcagaata cggcgaattt
    12061 cctgacgctc cagggcatca actgccgcag ccactgccag gtaggtttta cccgtacccg
    12121 ccgggccaac gccgaaggta atgtcatggt cgagaatatt ggcgatgtac tgcgcctggt
    12181 ttggcgtgcg cggcttaatt acgccgcgtt tagttttgat attgaccgct ttgccgtact
    12241 ccggcacgct ctccgcgctt tgctccagta cccgcgcttc tttaatcgca aggtggatct
    12301 gttccggttc gatatcctga atctgaccgc gcatcggggc agtatcgaca tacaggctac
    12361 gcagaatgtc tgccgcagcg gtgacgcaaa tcggacggcc ggtcagttta aagtggttat
    12421 cgcggcgatt gatctcgatg ccgagacggc gttcgagctg cttgatgttg tcatcaaacg
    12481 ggccgcacag gctcaacaga cgcgcattgt ctgctggctc cagggtgatt tcgcgagtgt
    12541 ctatgttcaa accgttcctc ttatctgtat gccgccggaa gctgaacatt caccggccta
    12601 taaggaaatt attcacgcca caggaaaaag gcgcaagcat tgcgataaag atggggataa
    12661 agagagaaaa aacaaggccc accggaacgg caggcctgag aattacggct gataataacc
    12721 cacgccaagg tcgttttctt tgcgggtgcg ggcaatcacc gactccggtg tttctgccat
    12781 gcgcagaccc atttcatctt cagttcgcac cactttaccg cgcagagagt tcgggtagac
    12841 gtcggtaatt tctacatcga cgaatttacc gatcatatcc ggtgtgccct cgaagttgac
    12901 cacgcggtta ttttcggtac gcccggaaag ctccatgatg ctcttacgcg aagtcccttc
    12961 taccagaata cgctgggtgg tgccgagcat ccgacggctc catgccatcg cttgctgatt
    13021 gatacgttct tgcagaatat acagacgctg cttcttctct tcttccggaa catcatcaac
    13081 catatcggcg gcaggcgttc ctggacgtgc agagaagata aagctgtagc tcatgtcgaa
    13141 attgacgtcg gcaatcagct tcatcgtttt ctcgaagtct tcggtggttt cgccagggaa
    13201 gccaacaatg aagtcagaac tgatctgaat atccggacgc gccgcacgca gtttacggat
    13261 gatcgctttg tactccagcg ccgtatgggt acgtcccatc aggttcagaa tgcgatcgga
    13321 accgctctgt accggcagat gcaggaagct caccagctca ggcgtgtcgc ggtacacttc
    13381 gatgatatcg tcggtgaatt cgatcggatg gctggtggta aagcgaatac gatcgatccc
    13441 gtcgatcgca gcaaccagac gcaacagatc ggcaaacgat ccggtggtgc cgtcgtagtt
    13501 ctcaccacgc caggcattca cattctgacc gagcaggttg acttcacgca cgccctgagc
    13561 cgcaagctgg gcaatctcaa acagaatatc gtcagacgga cggcttacct cttcaccacg
    13621 ggtgtaaggc accacgcagt aggtgcaata tttattgcag ccttccatga tggagacaaa
    13681 cgcggtcggc ccttcggcgc gcggttccgg cagacggtca aacttctcga tttccgggaa
    13741 gctgatatct acaaccgggc tgcggtcgcc gcgcacggag ttgatcatct ccggcagacg
    13801 gtgcagcgtt tgcggcccaa aaataatatc gacatagtgg gcgcgctggc gaatgtgctc
    13861 gccttcttgc gatgccacgc agccaccgac gccgataatc aggtctggat tcttctcttt
    13921 taacagtttc cagcgaccca actgatggaa gactttttcc tgagccttct cgcggattga
    13981 gcaggtgttc agcagtagca catccgcttc ttccgccacg tcggtcagtt gatagccgtg
    14041 ggtggcatcc agcagatcgg ccatcttcga tgaatcgtac tcgttcatct gacagcccca
    14101 ggttttaata tggagttttt tggtcatcga cttgctcttg cgaaatagta gccaggaatg
    14161 cagggcgcat agtgtaatgc tttgctgccg ttgtgaccag tatgagcgtt atcagccctt
    14221 gggggtaaaa atcctgtaaa cttaaagcat attgctaaca ggatgattga ccatgacaaa
    14281 tcaaccaacg gaaattgcca ttgtcggcgg aggaatggtc ggcggcgcac tggcgctggg
    14341 gctggcacag cacggatttt cggtaacggt gatcgagcac gcagaacctg caccgtttgt
    14401 cgctgacagc caaccggacg tgcggatctc ggcgatcagc gcggcttcgg tatcattgct
    14461 taaaggggta ggggtctggg atgcagtaca ggctatgcgt tgccatcctt accgcagact
    14521 ggaaacctgg gagtgggaaa cggcacatgt ggtatttgac gcagccgaat taaagttgcc
    14581 gttgcttggc tacatggtgg aaaacacagt cctgcaacag gcgttgtggc aggcgctgga
    14641 agcgcatccg aaagtaacgt tacgtgtgcc aacctcgctg attgcattac atcgagataa
    14701 tgatcttcag gagctggaac tgaagggcgg tgaaacgatc cgcgcgaaac tggtgattgg
    14761 tgccgacggc gcaaattctc aggtgcggca gatggcggga attggcgttc atgcctggca
    14821 gtatgcgcag tcgtgcatgt tgattagcgt acagtgtgag aacgatcccg gcgacagcac
    14881 ctggcagcaa tttaccccgg acggaccgcg tgcgtttctg ccgttgtttg ataactgggc
    14941 atcgctggtg tggtatgact ctccggcgcg tattcgccag ttgcagaata tgaatatggc
    15001 gcaactccag gcggaaatcg cgaagcattt cccgtcgcgt ctgggttacg ttacaccgct
    15061 tgccgctggt gcgtttccgc tgacgcgccg acatgcgttg cagtatgtgc agccggggct
    15121 tgcgctggtg ggcgatgccg cgcataccat ccatccgctg gcggggcagg gggtgaatct
    15181 tggttatcgt gatgtcgatg ccctgattga tgttctggtg aatgcccgca gctacggtga
    15241 agcgtgggcc agttatcctg tcctcaagcg ttaccagatg cggcgcatgg cggataactt
    15301 cattatgcaa agtggtatgg atctgtttta tgccggattc agtaataatc tgccgccgct
    15361 gcgttttgtg cgtaatctcg ggttaatggc ggcagagcgt gctggcgtgt tgaaacgtca
    15421 ggcgctgaaa tatgcgttag ggttgtagcc ttacaacatt gccggatgcg tgccaaccgt
    15481 aggtcggata agacgcggca gcgtcgcatc cgacattgaa ggataagacg tgtcaacatc
    15541 gcattcgaca ttgaatgaac gcagaaaagc aaaaagctcg ccgaagcgag cttttttaat
    15601 gtggctgggg tacgaggatt cgaacctcgg aatgccggaa tcagaatccg gtgccttacc
    15661 gcttggcgat accccaactg ggtgcactta cgatgtaagc gtcttgaata aattggctgg
    15721 ggtacgagga ttcgaacctc ggaatgccgg aatcagaatc cggtgcctta ccgcttggcg
    15781 ataccccaac aaattggttt ttgaatttgc cgaacgtatt cgacacattc agaatttggt
    15841 ggc
//
LOCUS       DAJTYF010000092        15827 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTYF010000092 DAJTYF010000000
VERSION     DAJTYF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.092"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..882)
                     /locus_tag="OO590_002970"
     CDS             complement(<1..882)
                     /locus_tag="OO590_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6638264.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGA"
     gene            989..1285
                     /locus_tag="OO590_002971"
     CDS             989..1285
                     /locus_tag="OO590_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6638265.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="OO590_002972"
     CDS             complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="OO590_002972"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6638266.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="OO590_002973"
     CDS             complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="OO590_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6638267.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="OO590_002974"
     CDS             complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="OO590_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6638268.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4298..4381
                     /locus_tag="OO590_002975"
     CDS             4298..4381
                     /locus_tag="OO590_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6638269.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4435..5787)
                     /gene="gorA"
                     /locus_tag="OO590_002976"
     CDS             complement(4435..5787)
                     /gene="gorA"
                     /locus_tag="OO590_002976"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6638270.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5859..6701)
                     /gene="rlmJ"
                     /locus_tag="OO590_002977"
     CDS             complement(5859..6701)
                     /gene="rlmJ"
                     /locus_tag="OO590_002977"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6638271.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6904..8946
                     /gene="prlC"
                     /locus_tag="OO590_002978"
     CDS             6904..8946
                     /gene="prlC"
                     /locus_tag="OO590_002978"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6638272.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRSGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8954..9706
                     /gene="rsmJ"
                     /locus_tag="OO590_002979"
     CDS             8954..9706
                     /gene="rsmJ"
                     /locus_tag="OO590_002979"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6638273.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9755..11224)
                     /gene="dtpB"
                     /locus_tag="OO590_002980"
                     /pseudo
     CDS             complement(9755..11224)
                     /gene="dtpB"
                     /locus_tag="OO590_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11530..11964)
                     /gene="uspA"
                     /locus_tag="OO590_002981"
     CDS             complement(11530..11964)
                     /gene="uspA"
                     /locus_tag="OO590_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6638274.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12355..12690
                     /gene="uspB"
                     /locus_tag="OO590_002982"
     CDS             12355..12690
                     /gene="uspB"
                     /locus_tag="OO590_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6638275.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12761..14260)
                     /gene="pitA"
                     /locus_tag="OO590_002983"
     CDS             complement(12761..14260)
                     /gene="pitA"
                     /locus_tag="OO590_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6638276.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14492..15691
                     /locus_tag="OO590_002984"
     CDS             14492..15691
                     /locus_tag="OO590_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6638277.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPVLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITT
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3982 a         4048 c         4153 g         3644 t
ORIGIN      
        1 tgcgccggca ctgccgatga ttaatgccat taatgctccc agacccattc cttttgtcat
       61 cagaacagat gccagaggga taaccgcctc agcccggata tacagcggaa taccgacaac
      121 ggcgcttaat gggatggcga agggattatc tgctcctgca tgagcggcaa tccacgctga
      181 gggaataaag ccataaataa aagatcctat taatactcct aacaaaagat atggcagtac
      241 atctttaaac tgttgcaacg catctttggt ggctagtttt atgggattaa tacttactac
      301 agctttggta ctgcaacagg taactacggg gttctcaatg gctttagccg ctggactaca
      361 acaactcact tttatgggcg tatatgtcgt tcccggcgaa gtttttgctg gagcacaaca
      421 atttgctgat gagcttttac tggcaatgat atgacgttca aaccccagag aatccaggat
      481 aatactggca agaacggaga cgccggctgc gataatcgcg tacaacaagg tgactttcca
      541 accaaaggtc atccacatta gtccgacgat aatgggatta agtaatggtg aaacaaataa
      601 aaaagtgaga gtcggaccaa acccggcttt cgctgacaac aatccacgta acatgggtat
      661 tgtcgagcaa ctacagaacg gggtaacggc tcccaacaga gcagccagga gatagcctct
      721 cccttttctg gcccccatca tctgctggat tttatggtct ggcacctttt gtcttatcag
      781 gctgacaccg gcactaatca ctataaacag caacgaaagc tcgacggcga gaaacacaaa
      841 catctctgcg gcatcttgca gcatcgcaag ccaactactc atacgtaacc tcattgggta
      901 aatctatatt tccagaataa tcgaaatgaa attcagatca agatatttct ggtattatcg
      961 aaatatcaac cagacagagg ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa
     1021 agagctaggt cacccgaccc gtttgttcat atttaaacat cttgtcaaag ccggggaaca
     1081 agggttgccg gtcggtgagt tgcaaaagca gcttgggata cctggctcca cactcagcca
     1141 ccatatatca gcactggtat cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct
     1201 gatgtgtgtt tcgcaatacg caatgctgga agcgataatt gaattcttac gcgaggagtg
     1261 ctgcgtaaat agcaaaattg cctgatgcgc tacgctctac atatttcatg ccatatattg
     1321 aatttgcacg gttttgtagg tcggataaga cgcgttagcg tcgcatccga cataaacaaa
     1381 gcgcacttta ccaactatcg gcagtggata tcgccgcttt atttcaggcg cttacccgct
     1441 tcatcaacca ctttctcgcc atcttccttg gagaatgcgc ctttttgcgc atctggcaga
     1501 atttccagca ccacttctga agggcggcac aggcgagttc ccagcggcgt caccacaatc
     1561 gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat
     1621 ttatcttccg caaggcccag ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt
     1681 acggaaatcc ccatatcggc aatgagtttg accagttcat cgcgcgttgg cggagtttcc
     1741 agataatgga taatggtcgg ttctgtgccg ctgttgcgga tcatctccag cgtattacgc
     1801 gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt atctcattac
     1861 aaagtgaaag agagacgtag cgccagcgca gccagcgtca caaacagcac aggcagggtc
     1921 atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt ctgcgaaagt
     1981 acgtgcagcc agagcagcgt tgccaggcta ccaatcgggg taattttcgg ccccaaatcg
     2041 cagccaatca cgttggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca
     2101 tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag
     2161 gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg
     2221 agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac
     2281 atgccgagcg agaagatgac aatctgccag ggggcaccgc gcaggacttt acccgtatta
     2341 atcgcatgac cgcgtttagc gacgacaaat aatatcagtg cgcccacagc tgcaatggcg
     2401 ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca
     2461 acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga tttcagcagc
     2521 gccatatcgt agttctgcgg aatatctttg cgaaaataga gatgtagcat caccagcgtg
     2581 gcaacaatcg cggcgatatc caccggcacc atcaccgagg cgtattcgcg aaagccgagg
     2641 ccaaagaaat cagcggaaac gatattcacc aagttggaga caataagcgg caggctggcg
     2701 gtatcggcaa tgaatccggc cgccatcacg aacgccagcg tcgtgccttt actgaatcct
     2761 aaagccagca gcatggcgat gacaatcggc gtcaaaataa gcgccgcgcc atcattggca
     2821 aacagggccg caacggcggc accgagcagg acaatccagg taaacagcaa gcgaccgcgt
     2881 ccattgcccc agcgtgaaac gtgcagcgct gcccattcaa aaaagccgga ctcatccagc
     2941 agcaggctga tgataatgac ggcaataaac gccgccgtcg cgttccagac gatattccac
     3001 accaccggaa tatcacccgg atgaaccacg cccgtaacca acgccagtac cgcgccgagc
     3061 gttgcactcc agccgatgcc taaacctttc ggctgccaga taaccaatac gatggtcagg
     3121 acaaagatag cgcctgccag taacataatg cctcccggat aaaacacatg tgcaaatcca
     3181 tatgtgttta gctaaatttt taactgcaaa tgttcttact gtctccggaa cagttttgtc
     3241 tggccaggtt gcggacaatt gcctgaatct tttcctgttc acatcgccag gcttcatcaa
     3301 taattttcgc cgcccatgct ggaatatgcg gcgataagcg gtaatgaacc cacttacctt
     3361 gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc cagatggcgg gatatcttgg
     3421 gctgcgactg atcgagggca gtgcagagat cgcagacgca taactctccc agttcgctaa
     3481 gcagtaaaac gatgcccaga cgggtttcat cagcaagaat tttgaacaat tggatgggta
     3541 acagaaatga catattgcgc tcctgattgt tgcaggtagt gtctctcttc gaagcggata
     3601 agtcaaaaac atatatgact taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa
     3661 ttttgagttg gctattcatt tgaaaggcgg tttgaatcag gacgtaaaaa aaaccgactt
     3721 tgcgtcggtt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
     3781 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
     3841 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
     3901 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
     3961 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
     4021 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
     4081 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
     4141 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
     4201 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
     4261 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
     4321 tcttggggcg ttaattgtgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
     4381 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
     4441 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
     4501 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     4561 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     4621 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     4681 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     4741 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     4801 cttgttatta aacaagcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     4861 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     4921 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     4981 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     5041 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     5101 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     5161 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     5221 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     5281 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     5341 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     5401 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     5461 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     5521 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     5581 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     5641 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     5701 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     5761 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     5821 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     5881 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     5941 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     6001 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     6061 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     6121 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     6181 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     6241 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     6301 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     6361 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     6421 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     6481 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     6541 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     6601 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     6661 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     6721 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     6781 aggccgccgc cgcgaaagcg taacgtttcc cattgaaatt cactacactt aaccccatgc
     6841 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     6901 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     6961 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     7021 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     7081 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     7141 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     7201 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     7261 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     7321 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     7381 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     7441 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     7501 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     7561 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     7621 agcacccgcg cctccgatca aggcccgaac gccggtaagt gggataacag caaggtgatg
     7681 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     7741 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     7801 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     7861 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     7921 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     7981 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     8041 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     8101 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcagc
     8161 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     8221 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     8281 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     8341 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     8401 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     8461 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     8521 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     8581 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     8641 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     8701 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     8761 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     8821 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     8881 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     8941 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     9001 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     9061 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     9121 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     9181 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     9241 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     9301 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     9361 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     9421 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     9481 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     9541 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     9601 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     9661 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     9721 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     9781 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     9841 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     9901 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     9961 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
    10021 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
    10081 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagtcg
    10141 tcaaaaagcc cagtgagcac ataaacatgc cgagagtaaa tttcatcggc atcgagaggt
    10201 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
    10261 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
    10321 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
    10381 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
    10441 tatccagctt gaatgcctga cgaaagaaga tgatggtgac gacgatggag agaacaatca
    10501 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
    10561 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
    10621 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
    10681 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
    10741 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
    10801 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
    10861 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
    10921 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
    10981 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
    11041 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
    11101 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
    11161 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
    11221 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
    11281 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
    11341 tattacaaca gaaaataacc agctgatcac actaatgatg gtgttaaatt ttgtattggc
    11401 aggagatttt gattgcgtac gcagatcgta ggccagataa ggggtttacg ctgcatcagg
    11461 taacaaggcg cgccaaaaag caaaacgccc gcatatgcgg gcgttcagga acacgtcgtg
    11521 gagggatgat tattcttctt cgtcgcgcag cggaacaatc agcatatcaa cgtgaacggt
    11581 gttgatcagc tgacgtgcgg aagacatcag tttgctccag aagtcctggt ggtgaccaca
    11641 aaccaccaga tccatatcgt atttcttgat tgcatcgacc agaacctggc ccaggtcgcc
    11701 gctgccgctc agggtttcag tgattggata gcctgcatta gtggaaagct cggtcagcgc
    11761 atgatgtgtc tcttcagaga tgcgtttctg catatcaccc agattcacat caataagccc
    11821 ggtgtatagg tcagagtagt ttacatctac gtggatcaga gaaactttcg cattgtaggg
    11881 gcgagccata gagactgctt tctctaccag aactttgctt tccggggaga gatcgactgc
    11941 gatgagaatg tgtttataag ccatagtgtt actccttcca taaagttgtc gatgactggc
    12001 cagctagcgt ttcttgtgcc gttacgtcac ccgcgtcctg cgaactacgt gcgcggggct
    12061 aacccagccg taaagattca gtgttaagac catccttacc ttatagcgac ccggatgatc
    12121 cgtcaatccg ccttgcttac ccgttagata aacaaatttt cgacatgaat atattgatag
    12181 tggttaacct tctggaaaaa aaacaacctg atctcctaca ctatctatag agccgctcgg
    12241 atgttggtca ccacaaaagc ggtttctcca ggacctggtg ttaactgatt ggcagcgtat
    12301 cgggagcggt agccccgggg tggaactccg cgggcaggtc gccggggagg aggtatgata
    12361 agcaccgtcg cattattttg ggctttatgt gtcgtttgca ttgttaacat ggcgcgctat
    12421 ttctcatcac tacgcgcgtt gttagtggta ctgcgtaact gcgatccatt gctctatcaa
    12481 tatgttgatg gagggggctt ttttacctca catggccaac ccaacaaaca ggtgcgtctc
    12541 gtttggtata tctatgccca acgttatcgc gatcatcacg atgatgagtt tattcgccgc
    12601 tgtgagcggg tgcgtcggca gtttattctg actagcgcat tgtgtggtct ggtggtggtc
    12661 agcctgattg cattgatgat ttggcattaa gcacttcagc aataataacg cataaaaaaa
    12721 gcgggccagt aaactgaccc gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga
    12781 accagtacag cccgccggaa agtagtaccg cagccggaag ggtaaacacc caagccatca
    12841 gaatactggt tacggtttta cgctgtaagc cgccgccatc taccaccatc gtccccgcga
    12901 cagaagagga gagtacgtga gtggtggaaa ccggcatccc ggtataactc gccaggccga
    12961 ttgacactgc cgccgtcatc tgggcagaca tcccctgagc gtaggtcatg cctttcttac
    13021 cgattttctc accgatagtc gttgccacac gacgccagcc aatcatcgta ccgataccta
    13081 acgccagcgc gaccgccatg atgatccaca ccggtgcata ctcgatggtg ctaagcatgt
    13141 cggacttcag tttcttcaac aggcgctgat cgtcagcact cacaccaggc atcttcacca
    13201 ctttgtcgat agtgtcagaa acgcacagca taatgcggcg catctggcta cgttgatcaa
    13261 gcgacagctt gtcgtagctt tccacatcgg tggtcaacat acctttcagg cggttgagcg
    13321 cgttaatggt attcgacgga tggcagtgga actccgcagg ttgcgttgcg ccagcttccg
    13381 gagccggtac taactgatca gcaccggtag cctgtttgag cagcgcagga tgctgctcaa
    13441 agtaagcttc gacgttgttg atggcatcac gggtacgggt gatttcgtag ccagtggcat
    13501 tcatgttcac cacgaagcct gctggcgcga cgccaatcaa taccaacata accagaccaa
    13561 tgcctttctg accatcgttc gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca
    13621 gcgcgatacg cgtccagaac ggcggctttt tcttgccgtc tttcttttca cgctccgctg
    13681 gggtcaggtg gatacgggcg cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa
    13741 tcagaccgcc agcaaacacc aggccgacaa taggggaaac gatcagagaa ccgaaaatac
    13801 ttaatacttt cgggatattg agtgcatcca ccactgacgt cccggtcatc aacgcattgg
    13861 ttaaaccaat cccgatgatc gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa
    13921 agtaccaggt acccaggttc cagataatcg ccgccagcaa catagagaac accatggcaa
    13981 ggccatgaga cgatcccata ttaagcagca gatccgtcgg cagcatatgc acaatggcat
    14041 aggcaacact cagaccaccc agcaaaacac ccaaaaagtt gaacaccgcc gccataacca
    14101 cggcgagctg agaacgcatc gcgcgggtat agataacggt tgccacggcg ttggctgtgt
    14161 catggaaacc attgatggct tcgtagaaca gcacaaaagc cagtgcaagc aataataaca
    14221 gcccggtatg caaatccagg ccagcaaaca aatgtagcat aggacgttac gccattttga
    14281 ggacatgaac gcggcgcatt atcagtgact ttcgcggcgc gggcaaagtg aaatatagac
    14341 tttttttgat ttgcctcctg tatggatttc actcaaaaaa taattatctt atataattca
    14401 ggcaaatact tccttttagt aatattgatg ctggtgcgac cactgaggaa tctttacaat
    14461 tcacgcccgt tttttctaag aggagcgcaa cgtggaaagg tttgatgcca ttattatagg
    14521 cgctggtgcg gcgggtatgt tctgttctgc gctggcaggt caggcaggac gccgggttct
    14581 gctgatcgat aatggtaaaa tactagggcg caaaatcctt atgtctggcg gtgggcgctg
    14641 caactttacc aacctttatg tcgaaccagg cgcttatctg agccagaatc cgcatttttg
    14701 taagtctgca ctcgcacgtt ttacccagtg ggatttcatt gatctggtca ataaacacgg
    14761 tatcgcctgg cacgagaaaa cgttagggca actcttctgc gatgactccg cgcagcagat
    14821 tgtcgacatg ctggtggatg agtgcgagaa gggcaatgta accttcagat tgcgtagcga
    14881 agtgctgagt gtggcgaagg atgaaacagg cttcacgctt gatctgaacg gcatgactgt
    14941 cggttgcgaa aagctggtca tcgcgaccgg tgggctgtca atgccggtgc tgggcgcatc
    15001 gccgtttggt tataagattg ccgaacaatt tggcctcaac gtgctgccga cccgcgcggg
    15061 tctggtgcca ttcactctgc ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt
    15121 ggcggtgcct tccgtgatta ccactgaaaa cggcaccgtt ttccgtgaga acttactctt
    15181 cacccaccgc ggcttatctg gaccggcggt gttgcagatt tcaagctact ggcaaccggg
    15241 tgaatttgtc agtatcaatc tgctaccgga tgtggacctc gaaaccttcc tgaatgagca
    15301 gcgtaacgca catccgaatc aaagcctgaa aaacacactg gcggttcatc taccgaagcg
    15361 gttggttgaa cgcttacagc aactcgggca aatcccggat gtttcgctaa aacagctcaa
    15421 cgtgtgtgac caacaggcac tgattagcac attgaccgac tggcgcgtac aacccaacgg
    15481 cactgaaggc tatcgcactg ccgaagtgac gctcggcggc gtggacacca acgaactttc
    15541 ttcacggacg atggaagcgc gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga
    15601 cgttaccggc tggctggggg gctataactt ccagtgggcg tggtcgagtg catgggcttg
    15661 tgcgcaggat ttggcgtcaa ttgtgaaata accatgacgt tttcccgtca gccgtttgta
    15721 tatgtcgtta ttaagtcatt ttggtacggt tctgcatccg acattctggt gttaactcgc
    15781 tgttttgtta gcgaattaac gctattgccg ttgtggttat tttatag
//
LOCUS       DAJTYF010000093        15428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTYF010000093 DAJTYF010000000
VERSION     DAJTYF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.093"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..1193)
                     /locus_tag="OO590_002985"
     CDS             complement(<1..1193)
                     /locus_tag="OO590_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6638278.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     FEVTH"
     gene            complement(1392..1476)
                     /locus_tag="OO590_002986"
     tRNA            complement(1392..1476)
                     /locus_tag="OO590_002986"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1440..1442),aa:Leu,seq:caa)
     gene            1672..2691
                     /gene="ahr"
                     /locus_tag="OO590_002987"
     CDS             1672..2691
                     /gene="ahr"
                     /locus_tag="OO590_002987"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6638279.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2820..4322
                     /locus_tag="OO590_002988"
     CDS             2820..4322
                     /locus_tag="OO590_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6638280.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4441..5523)
                     /gene="lptG"
                     /locus_tag="OO590_002989"
     CDS             complement(4441..5523)
                     /gene="lptG"
                     /locus_tag="OO590_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6638281.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5523..6623)
                     /gene="lptF"
                     /locus_tag="OO590_002990"
     CDS             complement(5523..6623)
                     /gene="lptF"
                     /locus_tag="OO590_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6638282.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPIRRLRASFSRKGAV"
     gene            6890..8401
                     /gene="pepA"
                     /locus_tag="OO590_002991"
     CDS             6890..8401
                     /gene="pepA"
                     /locus_tag="OO590_002991"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6638283.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8535..8978
                     /gene="holC"
                     /locus_tag="OO590_002992"
     CDS             8535..8978
                     /gene="holC"
                     /locus_tag="OO590_002992"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6638284.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8978..11833
                     /gene="valS"
                     /locus_tag="OO590_002993"
     CDS             8978..11833
                     /gene="valS"
                     /locus_tag="OO590_002993"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6638285.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11887..13083)
                     /locus_tag="OO590_002994"
     CDS             complement(11887..13083)
                     /locus_tag="OO590_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6638286.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSAGMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNSVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILTFLDLLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13276..13779
                     /locus_tag="OO590_002995"
     CDS             13276..13779
                     /locus_tag="OO590_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6638287.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13825..14241)
                     /gene="rraB"
                     /locus_tag="OO590_002996"
     CDS             complement(13825..14241)
                     /gene="rraB"
                     /locus_tag="OO590_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6638288.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14403..15416
                     /gene="argF"
                     /locus_tag="OO590_002997"
     CDS             14403..15416
                     /gene="argF"
                     /locus_tag="OO590_002997"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6638289.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3976 a         3935 c         3972 g         3545 t
ORIGIN      
        1 cagtgtgtca cttcaaacat caacctacgc tccccaagat gttccgcctt atggatgtaa
       61 gctgctcgca cagagctacg ctcctggtga ctcatctgcc gctctaccgc atccctcgac
      121 cataatcccg actcaatcaa cgaactacaa gccatcgtcc tgaagccatg cccacaaacc
      181 tcgaccttcg tgtcataccc cattacgcgc aaagccttgt tcaccgtatt ctcactcatc
      241 ggcttacgtg gatcgtgatc gcccacaaaa atcagctctc gatttccgct catgcttttg
      301 atcttttcca aaatacttag ggcctgccgc gacaaaggta caaggtgtgg tgtccgcatc
      361 ttcgagccac gctgagaatg cttgactcct tccagttgct cacgctcacc tgggattgtc
      421 cacatagccg tttcaaaatc tacttctgac cagcgggcaa aacgcagctc gctcgaacgg
      481 atgaaaacca ataaagtgag ttcaaccgcc agtcgagtta acggtctgcc agaatagtga
      541 tctatgcgat gaagtaattc aggtatacgg ttcagttcaa gagctgcacg gtgctgtctt
      601 ttcgccgtag caaccgcacc tgcaatctct tgcgcggggt tgtagtcgat taagccgctc
      661 tgaacggcaa agcgcataat cgcggtagta cgctgctgta aacgggcggc aacttcgagc
      721 cgcccggatg actcgactgc tttaatgggt acaagaagat cccgcgtctt cagttccgca
      781 atgttccgct taccgatagc agtaaagaga ttatcctcca gacttttcaa aacacgagcg
      841 ctatgcgatg ccgaccactt ctgattgctg gcgtgccagt ctctggcgac tacttcaaac
      901 gttatcgcct cttgttcctg ttctacctta acagctttct tgttttcact gggatcgaca
      961 ccattagcta aaagcttacg agcctcatcc cggcgtgccc tggcatccgc caacgacact
     1021 tcggggtatt tccccaatgc aagcatcttc tctttgccgc caaagcgata acgtagccgc
     1081 cagtatttgg agccgttagg gtgaaccagc aaaaccatgc cttcaccgtc ggtcagctta
     1141 taggcttttg cttcaggctt agccgaacga accttcacat cactcagagc catgatgagt
     1201 atcctttcaa gggttctgtg tgggtacaaa cattatcgaa ccgggaaata cccgcagttg
     1261 tacccgcatc agtaagttga tgtagattga atcaggttga cttaggttga gtgaaaaagc
     1321 gaggaaagcc ttgcggatac tggattttag gcacaaaaaa agacgtccgt tgacgtctat
     1381 tgatgttccg atggtgccga aggccggact cgaaccggca cgtatttcta cggttgattt
     1441 tgaatcaact gcgtctaccg atttcgccac ttcggcactg aagaggtatg cggaaaacgt
     1501 tgtggattat acctgtcgca cgccaccatg caagcgccag cttgcaccca cctcgctaag
     1561 tgctgaaatt ttcagcatta cactcagcga tatgtaacct ttgtcacact ccaggcaccc
     1621 cgccctgcca tgctctacac ttcccaaaca acaccagaga aggacccaaa aatgtcgatg
     1681 ataaaaagct atgccgcaaa agaagcgggc ggcgaactgg aagtttatga gtacgatccc
     1741 ggtgagctga agccacaaga tgttgaagtg caggtggatt actgcgggat ttgtcattcc
     1801 gatctgtcga tgatcgataa cgaatgggga ttttcacaat atccgctggt tgccgggcat
     1861 gaggtgattg ggcgcgtggt ggcactcggg agcgccgcgc aggataaagg tttgcaggtc
     1921 ggtcagcgtg tcgggattgg ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt
     1981 agcggtaatc agatcaactg cgagcaaggt gcggtgccga caattatgaa tcgcggtggc
     2041 tttgccgaga agttgcgtgc ggactggcaa tgggtgattc cactgccaga aaatattgat
     2101 atcgagtccg ccgggccgct gttgtgcggc ggtatcacgg tctttaaacc actgttgatg
     2161 caccatatca ctgctaccag ccgcgttggg gtaattggta ttggcgggct ggggcatatc
     2221 gctataaaac ttctgcatgc aatgggatgc gaggtgacgg cctttagttc taatccggcg
     2281 aaagagcagg aagtactggc gatgggtgcc gataaagtgg tgaatagccg cgatccgcag
     2341 gcactgaaag ccctgtcggg gcagtttgat ctcattatca atactgtgaa cgtcagcctc
     2401 gactggcagc cttattttga ggcgctgacc tatggcggta atttccatac ggtcggtgcg
     2461 gttctcacgc cgctgtctgt tccggccttt acgttaattg cgggcgaccg cagcatctct
     2521 ggttctgcta ccggcacgcc ttatgagctg cgaaagctga tgcgctttgc cgcccgcagc
     2581 aaggttgcgc cgacaaccga actgttcccg atgtcgaaaa ttaacgacgc cattcagcat
     2641 gtacgcgatg gtaaagcgcg ctaccgagta gtcctgaaag ccgacttctg atccctttgc
     2701 cgggtgctgt cgcccggcac ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt
     2761 agcggtgaag agtgcacagc cgttgcctat actccaggca ggaaactgga ggaaatctca
     2821 tgagcgaacc cctgttaatt gcccgcacgc cggacacaga actgttttta ctgccgggaa
     2881 tggctaaccg tcacgggctg attactggcg caacggggac gggtaaaacc gttacgctgc
     2941 aaaaactggc agagtcattg tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag
     3001 gcgatctgac cggtatcgcg caggcaggaa cggcgtcgga aaaactgctc acaaggctta
     3061 aaaatatcgg cgtcaatgac tggcaaccgc atgccaatcc ggtggtggtg tgggatatct
     3121 ttggcgagaa aggccacacg gtgcgggcga cggtttcgga tctggggccg ctgttgctgg
     3181 cgcgactgtt gaatctcaac gatgtgcaat ctggcgtgct gaatattatc ttccgcattg
     3241 cggacgatca gggattgttg ctgctcgact ttaaagattt gcgggcgatt acccagtaca
     3301 tcggcgataa cgccaaatcc ttccagaatc agtacggaaa tatcagtagc gcatcggttg
     3361 gtgccatcca gcgcggatta ctgtcgctgg aacagcaagg cgcagcacac ttctttggtg
     3421 agccgatgct ggatatcaaa gactggatgc gcaccgatgc caacggtaaa ggcgttatca
     3481 atatcctcag cgccgagaaa ctttatcaga tgccgaaact gtacgccgcc agcctgctgt
     3541 ggatgctttc agagttgtat gaacaattgc cggaagcggg cgatctggag aaaccaaaac
     3601 tggtgttttt cttcgacgag gcacatctgc tgtttaacga tgcaccgcag gtactgctgg
     3661 ataagattga gcaggtgata aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt
     3721 cgcaaaaccc gtctgatatt ccggataatg tgctcggcca gctcggtaat cgtgttcagc
     3781 acgctttgcg agcttttacg ccaaaagatc agaaagcagt gaagacagcg gcgcaaacca
     3841 tgcgggccaa tccggcattt gataccgaaa aggcaatcca ggaactgggg accggcgagg
     3901 cgttaatctc gtttctcgat gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga
     3961 tcgcaccttg ttcgcgaatg gggccggtga cggaagatga gcgtaatggc ctgattaatc
     4021 actccccggt gtatggcaaa tatgaagatg aggtggaccg cgagtccgcc tatgagatgc
     4081 tgcaaaaagg ctttcaggcc agtaccgagc agcaaaataa tccccccgtg aaagggaaag
     4141 aggtggcggt ggatgacggt attcttggtg gattgaagga tattttgttt ggcactaccg
     4201 gaccacgcgg cgggaagaaa gatggtgtgg tgcaaacaat ggcgaaaagc gccgctcgcc
     4261 aggtgacgaa tcagattgta cgcgggatgt tggggagttt gctggggggg agaaaaagat
     4321 aagtcagacg tgatctgtgc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg
     4381 ttcgaactcg tatttgactt tgtcaacaat aataaaggga gctttcgctc ccttgttaga
     4441 ttacgatttt ctcattaaca gccacaggct gattaagaag aagctggcac ttggcaacag
     4501 tgcgccgatg atcggcggga tgccgtaaac caacgtcagc gggccgaaga tctggtccag
     4561 tacgtagaag acaaaaccga agctgatacc tgtgaccaca cgcacgccca tcggtacgct
     4621 acgcagtggg ccaaagataa acgacagcgc catcagcatc atcaccgcca cggatagcgg
     4681 ctggaagatt ttgctccaca tgttgagctg ataacgtccg gcatcctgac cgctcgactt
     4741 cagatacttc acgtagttgt gcaaaccact gatggagagt gcatccgggt ccagcgccac
     4801 cacgcccagt ttgtctggcg tgaggtcggt tttccaggtg ccgctcaccg tctgcgaacc
     4861 ggtaatctgt ttcggattgg tcagatcaga ttcatcaacc tgcgacagac gccagacttt
     4921 atgttccggg tcaaacttcg cggtagcggc ataacgtacg gattgcagac gacgattctc
     4981 gttaaaggca taaatactga tgccacctaa cacctcgtca cctttaaccc gctcaatgta
     5041 gacgaagttg ttgccatctt tcgcccataa accttgctgg gtagagagca acgagccgcc
     5101 gtacatcgcc tgcgcacggt agttacgcgc catctgttcg ccctgcggcg cgacccattc
     5161 gccaatcgcc atcgtcagca agaccagtgg aatggcggtt ttcatcaccg acagcgctac
     5221 ctgcatacgg gtaaaaccag aagcctgcat caccaccagt tcgctgcgct gcgccagcat
     5281 cccaagacca agcaacgccc caagaagagc cgccatcggg aagaagatct gcacatcttt
     5341 cggcacgctc agcaaggtat acattcctgc gcctaacgcg tcgtaactcc cctgcccggc
     5401 ttttttcagc tgatcgacaa atttgataat gcccgacagc gacaccagca tgaacagcgt
     5461 catcatgatg gtggtgaaaa tagttttacc gatatagcgg tcaagtacgc caaaaggttg
     5521 catcacaccg ctcctttacg cgaaaaactg gcgcgcaggc ggcggatcgg cacggtgtcc
     5581 caaaggttga gaacaatcgc caaagccaga taaatcaggt taacggtcca catccacagt
     5641 gtcgggtcca gcttaccttt accgccgttc gatttcaggg aggtctggat caggaagaaa
     5701 agtagataca gcagcatggc tggcagcatc gacagtacgc gtccctgacg cgggttaacc
     5761 acgctcaacg gtacaaccat aagtgccatc ataaacacgg tgaataccaa cgtgatacgc
     5821 cagttcagtt ctgcgcgagc acgatcggtg tcagtgttcc acaatgtgcg catgtccatc
     5881 tggtcggtat cgttcgggtc gagcgccacc gcctggtgac caatgatcgc ctgataatcc
     5941 tggaaatccg taatgcggaa atcacgtaac agtgcagtgc cttcgaagcg tgttccctgg
     6001 ttgagagtga cgacctggga gccgtcgcgc agctgggtta aatgtccgga atcggccacc
     6061 accacagaag gacgtgcatt accttttggt cgaatttgtg cgaggaacac atctttgaaa
     6121 tcgctgccgt caacgctttc gatgaacagc accgagctgc cattagtcgc ttgctggaat
     6181 tgcccttgcg ccagcgccgc catgccaggg ttcgctttcg cttctgctaa cacttcatcc
     6241 tgatgacgcg atgaccacgg tcccgcccac atcacgttaa ccgccgcgac gattgccgtg
     6301 aataccgcaa ggatcattgc cgctttcacc agaaccgctt tgctcaagcc gcaggcatgc
     6361 attaccgtaa tttcactttc ggtatacagt ttgcccagcg tcatcagcag cccgaggaac
     6421 aggcttaatg gcaggataag ctgcgccatt tccggcacgc ccaacccgag aagggagagc
     6481 accagattcg ccggaatatc gccgtcaacc gctgcgccga ggatcctcac taacttttga
     6541 cagaagaaga tcaaaagcaa gatgaagagt atcgccagct ggcttttgag cgtctcccgc
     6601 accagatatc ttatgattat cactttaaat acgcccgtaa aaactcgtct tttgcaggat
     6661 tttagcttgt ttcatggctt aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta
     6721 agataattat actcaacgga ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa
     6781 taacgttaag attaacacga agtcatcgca acagcggaca tgagttacga aagcttgcaa
     6841 ttctatctgt agccaccgcc gttgtcttta agattcagga gcgtagtgca tggagtttag
     6901 tgtaaaaagc ggtagcccgg agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga
     6961 accacgtcgc ctttctccga ttgcagaaca gctcgataaa atcagcgatg ggtacatcag
     7021 cgccctgcta cgccggggcg aactggaagg aaaaccgggg cagacattgt tgctgcacca
     7081 tgttccgaat gtactttccg agcgaattct ccttattggt tgcggcaaag aacgtgagct
     7141 ggatgagcgt cagtacaagc aggttattca gaaaaccatt aatacgctga atgatactgg
     7201 ctcgatggaa gcggtctgct ttctgactga actgcacgtt aaaggccgta acaactactg
     7261 gaaagtgcgt caggctgtcg agacagcaaa agagacgctc tacagtttcg atcagttgaa
     7321 aacgaataag agcgaaccgc gtcgtccgct gcgtaaaatg gtgttcaacg tgccgacccg
     7381 ccgtgaactg accagcggtg agcgcgcgat ccagcacggt ctggcgattg ccgccgggat
     7441 aaaagcagcg aaagatctcg gtaatatgcc gccgaatatc tgtaacgccg cttatctcgc
     7501 ctctcaggcg cgccagctgg ctgacagcta cagcaagaat gtcatcaccc gcgttatcgg
     7561 cgaacagcag atgaaagagc tgggaatgca ctcttatctg gcggtcggtc agggttcaca
     7621 aaacgaatcg ctgatgtcgg tgattgagta caaaggcaac gcgtcggaag atgcacgccc
     7681 aatcgtgctg gtgggtaaag gtttaacctt cgactccggc ggtatctcca tcaagccttc
     7741 agaaggcatg gatgagatga agtacgatat gtgcggcgcg gcggcggttt acggcgtgat
     7801 gcgtatggtc gcggagctgc aactgccgat taacgttatt ggcgtgctgg caggctgtga
     7861 aaacatgcct ggcgggcgtg cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca
     7921 aaccgttgaa gtgctgaaca ccgatgccga aggccgcctg gtactgtgcg acgtgttaac
     7981 ttacgttgaa cgttttgagc cggaagcagt gattgacgtg gcgacgctga ccggtgcctg
     8041 cgtgatcgcg ctgggtcatc atattactgg tctgatggcg aaccataatc cgctggccca
     8101 tgaactgatt gccgcgtctg aacaatccgg tgaccgcgca tggcgcttac cgctgggtga
     8161 cgagtatcag gaacagctgg agtccaattt tgccgatatg gcgaacattg gcggtcgtcc
     8221 tggtggggcg attaccgcag gttgcttcct gtcacgcttt acccgtaagt ataactgggc
     8281 gcacctggat attgcaggaa ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg
     8341 tccggtagcg ttgctggcac agttcctgtt aaaccgcgct gggtttaacg gcgaagagta
     8401 agtttgcgtt ttaccctcac cctaaccctc tccctaaggg agagggaacc gatcgagcgc
     8461 aatagaaatt cattgctcat ctttcagggt gaggaatgca gcaatattta aatccaccac
     8521 aagaagcccc atatatgaaa aacgcaacgt tctaccttct ggacaatgac accaccgtcg
     8581 atggcttaag cgccgttgag caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg
     8641 gtaagcgcgt actcatcgcc tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc
     8701 tgtgggcgcg tccggcagaa agctttgttc cgcataattt agcgggagaa ggaccgcgcg
     8761 gcggtgcgcc ggtggagatc gcctggccgc aaaaacgcag cagcagcccg cgcgatatat
     8821 tgattagtct gcgaacaagc tttgcagatt ttgccaccgc tttcacagaa gtggtagact
     8881 tcgttcctta tgaagattct ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg
     8941 tggctggttt caacctgaat acggcaacct ggaaataatg gaaaagacat acaacccaca
     9001 agatatcgaa cagccgcttt acgagcactg ggaaaagcag ggctacttta agcctaatgg
     9061 cgatgaaagc caggaaagtt tctgcatcat gatcccgccg ccgaacgtca ccggcagttt
     9121 gcatatgggt cacgccttcc agcaaaccat catggatacc atgatccgct atcagcgcat
     9181 gcagggcaaa aacaccctgt ggcaggtcgg tactgaccac gccgggatcg ctacccagat
     9241 ggtcgttgag cgcaagattg ccgcagaaga aggtaaaacc cgtcacgact acggccgcga
     9301 agctttcatc gacaaaatct gggaatggaa agcggaatct ggcggcacca ttacccgtca
     9361 gatgcgccgt ctcggcaact ccgtcgactg ggagcgtgaa cgcttcacca tggacgaagg
     9421 cctgtccaat gcggtgaaag aagttttcgt tcgtctgtat aaagaagacc tgatttaccg
     9481 tggcaagcgc ctggtaaact gggacccgaa actgcgtacc gctatttctg acctggaagt
     9541 ggaaaaccgc gaatcgaaag gttcgatgtg gcacatccgc tatccgctgg ctgacggcgc
     9601 gaaaaccgca gacggtaaag attatctggt ggttgcgacc actcgcccgg aaaccctgct
     9661 gggcgatacc ggtgtagctg ttaacccgga agatccgcgt tacaaagatc tgattggcaa
     9721 atatgtcatt ctgccgctgg ttaaccgtcg tattccgatc gttggcgacg aacacgccga
     9781 catggaaaaa ggcaccggct gcgtgaagat caccccggcg cacgacttta acgactatga
     9841 agtcggtaaa cgtcacgccc tgccgatgat caacatcctg acctttgacg gtgatatccg
     9901 tgaaagcgcc caggtgttcg ataccaaagg taacgaatct gacgtttatt ccagcgagat
     9961 cccggcagag ttccagaaac tcgagcgttt tgctgcacgt aaagcagtcg ttgctgcggt
    10021 tgatgcactc ggcctgctgg aagaaattaa accgcacgac ctgaccgttc cttacggcga
    10081 ccgtggcggc gtggttatcg aaccgatgct gaccgaccag tggtacgtgc gtgccgatgt
    10141 actggcgaaa ccggcagttg aagcggttga gaacggcgac attcagttcg taccgaagca
    10201 gtacgaaaac atgtacttct cctggatgcg cgatattcag gactggtgta tctctcgtca
    10261 gctgtggtgg ggtcaccgta tcccggcatg gtatgacgaa gcgggtaatg tttatgttgg
    10321 ccgcaacgaa gaagaagtgc gcaaggaaaa taacctcggt gccaacgttg ccctgcgtca
    10381 ggacgaagac gttctcgaca cctggttctc ttctgcactg tggaccttct ctacccttgg
    10441 ctggccggaa aataccgacg ccctgcgtca gttccaccca accagcgtga tggtatctgg
    10501 tttcgacatc atcttcttct ggattgcccg catgatcatg atgaccatgc acttcatcaa
    10561 agatgaaaat ggcaaaccgc aggtgccgtt ccacactgtt tacatgaccg gtctgattcg
    10621 tgacgacgaa ggtcagaaga tgtccaaatc caagggtaac gttatcgacc cgctggatat
    10681 ggttgacggt atttcgctgc cagaactgct ggaaaaacgc accggcaata tgatgcagcc
    10741 gcagctggcg gacaaaatcc gtaagcgcac cgagaaacag ttcccgaacg gcattgagcc
    10801 gcacggcacc gatgccctgc gcttcaccct ggcggcgctg gcgtctaccg gtcgtgacat
    10861 caactgggat atgaagcgtc tggaaggcta ccgtaacttc tgtaacaagc tgtggaacgc
    10921 cagccgcttt gtgctgatga acacagaagg tcaggattgc ggcttcaacg gcggcgaaat
    10981 gacgctatcg ctggcggacc gctggattct ggcggagttc aaccagacta tcaaagcgta
    11041 ccgcgaagcg ctggacagct tccgcttcga tatcgccgca ggcattttgt atgagttcac
    11101 ctggaaccag ttctgcgact ggtatctgga gctgaccaag ccggtaatga acggtggaac
    11161 agaagccgaa ctgcgcggta ctcgccatac gctggtgact gtactggaag gtctgctgcg
    11221 cctcgcgcat ccgatcattc cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact
    11281 ttgcggtatc actgccgaca ccatcatgct gcagccgttc ccgcagtatg atgcatctca
    11341 ggttgatgaa gccgcactgg ccgacaccga gtggctgaaa caggcgatcg ttgctgtacg
    11401 taacatccgt gcagaaatga acatcgcgcc gggcaaaccg ctggagctgc tgctgcgtgg
    11461 ttgcagcgcg gatgcagaac gtcgcgtaaa tgaaaaccgt ggcttcctgc aaacgctggc
    11521 gcgtctggaa agcatcaccg tgctgcctgc cgatgacaaa ggtccggttt ccgttaccaa
    11581 gatcatcgac ggcgcagagc tgctgatccc gatggctggc ctcatcaaca aagaagatga
    11641 gctggcgcgc ctggcgaaag aagtggcgaa gatcgaaggt gaaatcagcc gcatcgagaa
    11701 caagctggca aatgaaggct ttgttgcccg cgcaccggaa gcggttatcg cgaaagagcg
    11761 tgagaagctg gaaggttatg cagaagcgaa agccaagctg attgaacagc aggctgttat
    11821 cgccgcactg taatcaacac agtgatgaaa acgaaggccg gagcatgctc cggccttttt
    11881 tatctcttac agaaatggta aagaaggcat gactccccga gaaatactgg ctaagaagcc
    11941 tttatctggt tgttcttctt tattgattaa aggaaggtca tccaaatcgc ccagcaaata
    12001 cgtatttttt gcctgccagt caatcatcca tattttcagc agtgggtaag ccagaccacc
    12061 cgtaatcccg gatatcacca ccaacgcaca catgcgataa agcataccgt ggtacgttaa
    12121 agttgagcga aaacgaatac gcccatcatt cagtgacagg ttgctcataa aatgatttcg
    12181 caacgacacc gttaaataac tggtgctaac agcaatccca aaataataaa ttaactgcgc
    12241 gattatcatt ttacgttgca tttccataaa ttgctgtaaa ttctcaatat catcatttgc
    12301 atacccagaa ctatcatact catttaatat ttgatcgaag ataatataac cagcaacagc
    12361 gagaaaaggt aataaatcta aaaatgtgag gatggcatat tttatacaat acgcagtatc
    12421 taatttcacc ttgaaacgat gtataccgaa actggtattg ctccagagaa aactcattac
    12481 caggctatat aaagtaccat taaaaatacc aatggaaaca ataccaacta ttgccatcag
    12541 aaccacagat ataataacac tacttgaact attggcatgt agcatctttg tcgagataaa
    12601 gaaaacagtc cccatcccaa tggccattaa aatcggcaag aaaaaggtta cccaccagaa
    12661 ccctttcaaa gagcaattaa aactaaatct tacgctgttg agactggtca ttaaggcctg
    12721 ataacgcagt ccttttgctg ccataaatat aagcaaaacc aacagtaata aagtcaaaac
    12781 acagccaaca agcggcatac ctgctgacac tgtcattaag attgcgaaat agaaaaaaac
    12841 aaaaacaaga caactaacaa aaacattccc accggtaatt ccataagaaa atcgttgtcc
    12901 gttaacttcc atattagcat aaagataacg cttacatttc attaatgccc atggtaaata
    12961 gatacctagc gttataatcg ttaacaacac attcaccaca caaataagaa aatatctctc
    13021 acctgtacca tgaaaaacaa aagaatggga cggaacatcc atttcattaa taacttgagc
    13081 cataaaaatc ccctgaatta aaaaatccat tgatataatt tatagataca gacagtaaaa
    13141 tgttctgcca cgttacaata ctcttatcag taataggctg gcaaaaactt tattgcttta
    13201 taagacgctt gcgcacgtaa gaagagagtg gtattaaaca aatgtataat tactttattt
    13261 ttattgggtt aaactatgaa taacattgcg ccgcaatcac ctgtaatgcg tcgcctgacg
    13321 ctgcaggata atcctgctat cgccagcgtc atccgtcagg tttacgcaga gtatggtctt
    13381 accgccgata aaggctacac cgttgccgat ccgaatctcg acgaactgta tcaagtgtac
    13441 agccagcctg gccatgctta ttgggtggtt gaatacaatg gcgaagtggt cggcggcggc
    13501 gggattgcgc cattaacagg tagtgaagcg gacatttgcg aactgcagaa aatgtacttt
    13561 cttcccgcta ttcgcggtaa agggctagcg aaaaaactgg ccttaatggc gatggagcag
    13621 gctcgagaga tgggcttcaa acgctgctat ctggaaacga ccgctttttt aaaggaagcc
    13681 attgcgcttt atgagcattt gggctttgag catatcgact atgcgcttgg ctgcacgggc
    13741 catgtcgatt gtgaagtgcg gatgctacgc gaactctaaa aaaatgcccg ctagtatgtg
    13801 ctatcgggca ttttaatcaa ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa
    13861 atcttcatcg tcgccatctt cgccgttagg atcttcaaag taagtgcccc aaccgtcgta
    13921 ctcaacgtca aatttctccg ccagcgtcat cagttgttca acctgggcat cgatcagatc
    13981 ggcattcaac gcgcactcgc tgaggatgtc gcagcaaatc acgatatcac catcttcaac
    14041 ttccagctct tctggatcgg tcacttcgta accgagttta aacgcttcaa ctgccgcttt
    14101 ttccagagtt tccagatcgt ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg
    14161 atcgctgcca tcttccagta attcttcaat aatcaaacgt gtttcttcac gctgttcttc
    14221 cagttgttcc gggtttgcca tggctcattc ctcttaatgt cctgccgata ctcttattgt
    14281 cacacaccac tgacattgcc tccacctttg tgacaaagat ttatgcttta gacttgcaaa
    14341 tgaataatca tacacataaa tttaatttta attcattgag gcgttagcca caggagggat
    14401 ctatgtccgg gttttatcat aagcatttcc tgaaattact cgatttcacg ccagctgaac
    14461 tcaacagcct gctgcagtta gccgcgaagc tgaaagctga taagaaaagc ggtaaagaag
    14521 aagccaaact cactggtaaa aacatcgcgc tcatcttcga aaaagactcg actcgtaccc
    14581 gatgctcttt cgaagttgcc gcatatgacc agggtgctcg cgttacttat cttggcccaa
    14641 gcggtagtca gattggtcat aaagagtcga ttaaagacac tgcccgcgtg cttggtcgca
    14701 tgtatgacgg tattcagtat cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat
    14761 acgctggcgt gccggtatgg aacggcctga ccaatgagtt tcaccccacg cagttgctgg
    14821 cggatctcct caccatgcag gagcatttac ccggtaaagc attcaacgaa atgacgctgg
    14881 tctatgcagg tgacgcacgt aacaacatgg gcaattcgat gctcgaagct gcggcgctta
    14941 ccggtctgga tttgcgtctg gtcgcgccac aggcatgctg gccagaagct gcgctggtta
    15001 cggaatgccg cgccctggca caacaaaatg gcgggaatat tacgctgacg gaagatgtag
    15061 ctaaaggagt tgaaggtgct gactttatct ataccgatgt atgggtgtcg atgggtgaag
    15121 caaaagaaaa atgggctgaa cgcattgcat tactgcgtga ttatcaggtg aacagcaaga
    15181 tgatgcagtt gaccggtaac ccggaggtca aattcctcca ctgcctgccc gcgtttcatg
    15241 acgaccaaac gacgcttggc aaaaaaatgg cggaagagtt tggcctacat ggcggaatgg
    15301 aagtgactga tgaggtcttc gagtctgccg ccagcattgt atttgatcag gcggaaaacc
    15361 gcatgcatac catcaaagcg gtgatggtcg cgacgctcag taaattaaat aattaatatc
    15421 tctataaa
//
LOCUS       DAJTYF010000094        14915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTYF010000094 DAJTYF010000000
VERSION     DAJTYF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.094"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            597..794
                     /locus_tag="OO590_002998"
     CDS             597..794
                     /locus_tag="OO590_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638290.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO590_002999"
     CDS             complement(744..1937)
                     /locus_tag="OO590_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6638291.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO590_003000"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO590_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6638292.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO590_003001"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO590_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6638293.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO590_003002"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO590_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6638294.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7800
                     /gene="iucD"
                     /locus_tag="OO590_003003"
     CDS             6484..7800
                     /gene="iucD"
                     /locus_tag="OO590_003003"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6638295.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7806..10001
                     /gene="iutA"
                     /locus_tag="OO590_003004"
     CDS             7806..10001
                     /gene="iutA"
                     /locus_tag="OO590_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6638296.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10552..10695)
                     /locus_tag="OO590_003005"
     CDS             complement(10552..10695)
                     /locus_tag="OO590_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638297.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10945..14832
                     /gene="sat"
                     /locus_tag="OO590_003006"
     CDS             10945..14832
                     /gene="sat"
                     /locus_tag="OO590_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCR6638298.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         4018 a         3553 c         3705 g         3639 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatggctcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg tcgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagaa gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccc tgcttcttta actcactata cagcaaatat tcaggaataa
     7801 cgatgatgcg caaaaagtat atgccccggg ctcttggtcc gctgcttctt gtcgtgctgt
     7861 caccagctgt cgcccagcaa aacgatgata atgagatcat agtgtctgcc agccgcagca
     7921 atcgaactgt agcggagatg gcgcaaacca cctgggttat cgaaaatgcc gaactggagc
     7981 agcagattca gggcggtaaa gagctgaaag acgcactggc tcagttaatc cccggccttg
     8041 atgtcagcag ccaaagtcga accaactacg gtatgaacat gcgtggccgc ccgctggttg
     8101 tcctgattga cggtgtgcgc ctcaactctt cacgttccga cagccgacaa ctggactctg
     8161 tcgatccttt taatatcgac catattgaag tgatctccgg cgcgacggcc ctgtacggtg
     8221 gcgggagtac cggagggttg atcaacatcg tgaccaaaaa aggtcagccg gaaaccatga
     8281 tggagtttga ggctggcaca aaaagtggct ttaacagcag taaagatcac gatgagcgca
     8341 ttgccggtgc tgtctccggc ggaaatgacc atatctccgg acgtctttcc gtggcatatc
     8401 agaaatttgg cggctggttt gacggtaacg gcgatgccac cctgcttgat aacacccaga
     8461 ccggcctgca gcactccaat cggctggaca tcatgggaac cggtacgctg aacatcgatg
     8521 aatcccggca gcttcaactg ataacgcagt actataaaag tcagggagac gacaattacg
     8581 ggcttaatct cgggaaaggc ttttccgcca tcagcgggag cagcacacca tacgtcagta
     8641 aggggctgaa ttctgaccgc attcccggca ctgagcggca tttgatcagc ctgcagtact
     8701 ctgacagtga tttcctggga caggaactgg tcggtcaggt ttactaccgc gatgagtcgt
     8761 tgcggtacta cccgttcccg acggtaaatg cgaataaaca ggcgacggct ttctcctcgt
     8821 cacagcagga taccgaccag tacggcatga aactgactct gaacagccaa ctgatggacg
     8881 gctggcaaat cacctggggg ctggatgctg agcatgagcg ctttacctcc aaccagatgt
     8941 tcttcgatct ggctcaggca agtgcttccg gagggctgaa caaccataag atttacacca
     9001 ccgggcgcta tccgtcatat gacatcacca atctggcggc cttcctgcaa tccagctatg
     9061 acattaatga tatttttacc gttagcggtg gcgtacgcta tcagtatact gagaacagag
     9121 tagatgattt catcgactac acgcagcaac agaagattgc tgccgggaag gcgatatctg
     9181 ccgacgccat tcctggtggt tcggtagatt acgataactt tctgttcaat gctggtctgc
     9241 tgatgcacat caccgaacgt cagcaggcat ggttcaattt ttcccagggg gtggcattgc
     9301 cggatccggg gaaatattat ggtcgcggca tctatggtgc agcagtgaac ggccatcttc
     9361 ccctgacaaa gagcgtgaac gtcagcgaca gtaagctgga aggcgtgaaa gtcgattctt
     9421 atgaactggg ctggcgcttt atcggtgaca acctgcggac tcaaatcgcg gcatattact
     9481 cgctttccaa taagagcgtg gaaaggaata aagatctgac catcagtgtg aaggacgaca
     9541 ggcgccgtat ttacggcgtg gaaggtgcgg tggactacct gatcccggat actgactgga
     9601 gtaccggtgt gaacttcaat gtgctgaaaa ccgagtcgaa agtgaacggt caatggcaaa
     9661 aatatgacgt gaaggaatca agtccatcga aagcgacagc ttacattaac tgggcgccgg
     9721 aaccgtggag tctgcgtgta cagagcacca cttctttcga cgtaagcgat gcagagggta
     9781 acgatattaa tggttacact accgtcgatt ttatcagtag ttggcagctt ccggtgggaa
     9841 cactcagctt cagcgttgag aacctcttcg accgtgacta taccactgtc tggggacagc
     9901 gtgcacctct gtactacagc ccgggttacg gccctgcttc actgtacgac tacaaaggcc
     9961 ggggccgaac ctttggtctg aactactcag tgctgttctg acggggatgc ttttgcaaca
    10021 agggtatgct gtaaacaaca tggtccttgg tggccatgtt gtttacagcc agtataatat
    10081 acagccttaa caagatattc cgtctccgga atgggctgtt caccactgaa taacattatg
    10141 tctgttttat ggatctttga ctgaaattta tcgcatatcc tcatttcatt gtccgtctga
    10201 aaactaacct tacaagtaag cgtcgtctca gcaccgtctg gcagatcctg aaattcctga
    10261 gaggatgatc tgcgcccaat atcctgagcc ggaactttgc tgcaatgaag gccaacgaaa
    10321 aatgggggag tatgttatag agtacttgct acagggtaaa aagaggtacc tgtcgctggt
    10381 tctccgttca gtttaaccgg gagattttct cctgcgcctg tcataaagtc cggtaatgga
    10441 gattgttaat acgatggtgc acgatgcatc gtcagtgccc ggcccggagg atgccccgta
    10501 gctacatacg gatcagggtt ggcaatatcg aatggcaggc tataggcaaa gttaaagccc
    10561 catggagtag cacaaaatat gccgcacaaa ggaaacggtc tgaataacgc agtgatggag
    10621 aacttcttcg gcacactgca aaatcgcatc gtggtcgatc gctaattcgt cgtgaatcac
    10681 tgaacgatgt tccatgcgaa cagatattcg atcatatcga aatattaaac attacccctc
    10741 cccacattgg gcaaaaaaaa acaaaaaacg atcaattaac gatcaattag tattgattca
    10801 cataatctct catcattaat gagaacattg tcacatattt acaatataga actgttactt
    10861 ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata ctttgttcat
    10921 tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata tagtgctgcc
    10981 actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa aacaaaccga
    11041 aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc agcaaatatt
    11101 gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa aggtattttc
    11161 cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt ctctttccat
    11221 aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac cgcaatagga
    11281 ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc agaaactcag
    11341 gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga ttttgagatt
    11401 cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggaaga gaccaaccct
    11461 acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg ttcaaaaaaa
    11521 atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga atccaaaatc
    11581 tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga ggtcacccaa
    11641 tgggactcat acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata
    11701 ttcggagaca gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta
    11761 gtcggaacaa cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa
    11821 tacaatgata aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat
    11881 ggcaataatg taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc
    11941 actggtactc aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat
    12001 gttctgttta aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat
    12061 gaatataaca taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa
    12121 gaaagcattg taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc
    12181 cccggtactc tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat
    12241 gttattctta atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag
    12301 gtaatactaa ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact
    12361 aaacgtggtg gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc
    12421 actgacgatg gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc
    12481 aataacgaag actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac
    12541 aatattaatt ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac
    12601 acaaaaaatg atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag
    12661 catgcaatat tcagaagcac aacgagtcat tgctccctgg tatttctttg tggaacggac
    12721 tgggtcaccg ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac
    12781 aaaagtaata atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa
    12841 tttgatacat tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa
    12901 ggaaatatat cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat
    12961 aatcttgcag ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact
    13021 aatctatcca taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata
    13081 gccataggtg atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct
    13141 ataagtatag ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt
    13201 attgatatct ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta
    13261 acgccgggtc tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc
    13321 attgccagaa atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt
    13381 actctgggac agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca
    13441 gagactcttt tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct
    13501 acagttagca tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta
    13561 gaaacaaaag acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca
    13621 gtggacaacc ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca
    13681 gatcagcttg ttgtcaataa atcgttgtct ggtaaaaaca accttctgtt agtcgacttc
    13741 attgagaaaa atggaaacag caacggactg aatatcgatc tggtcagcgc accaaaagga
    13801 actgcagtag atgtctttaa agctacgact cggagtattg gcttcagtga tgtaacaccg
    13861 gttatcgagc aaaagaacga tacagacaaa gcaacatgga ctctgatcgg ctataaatct
    13921 gtggccaacg ccgatgcggc taaaaaggca acattactga tgtcaggcgg ctataaagcc
    13981 ttccttgctg aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga cattaacggt
    14041 gagtccggtg catgggcccg aatcatgagc ggaaccgggt ctgccggcgg tggattcagt
    14101 gacaactaca cccacgttca ggtcggtgcg gataacaaac atgaactcga tggccttgac
    14161 ctcttcaccg gggtgaccat gacctatacc gacagccatg caggcagtga tgccttcagt
    14221 ggtgaaacga agtctgtggg tgccggtctc tatgcctctg ccatgtttga gtccggagca
    14281 tatatcgacc tcatcggtaa gtacgttcac catgacaacg agtataccgc aactttcgcc
    14341 ggccttggca ccagagacta cagctcccac tcctggtatg ccggtgcgga agtcggttac
    14401 cgttaccatg taactgactc tgcatggatt gagccgcagg cggaacttgt ttacggtgct
    14461 gtatccggga aacagttctc ctggaaggac cagggaatga acctcaccat gaaggataag
    14521 gactttaatc cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt ctccggtaag
    14581 gactggaaag tcacagcccg cgccggcctt ggctaccagt ttgacctgtt tgccaacggt
    14641 gaaaccgtac tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa agacggtcgt
    14701 atgctcatga atgttggtct caatgccgaa attcgcgata atcttcgctt cggtcttgag
    14761 tttgagaaat cggcatttgg taaatacaac gtggataacg cgatcaacgc caacttccgt
    14821 tactctttct gataacagcc cgggccgcgt ttgcggccct tcttctaccg gagagaatat
    14881 gtattaccct gtgacagact atatcgctct tgctc
//
LOCUS       DAJTYF010000095        14843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTYF010000095 DAJTYF010000000
VERSION     DAJTYF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.095"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..235
                     /locus_tag="OO590_003007"
     CDS             <1..235
                     /locus_tag="OO590_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCR6638299.1"
                     /translation="AHTPASLLTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLF
                     VEQPDLSQLRDLVKTGATRKIRSEARHRLEEKQ"
     gene            232..687
                     /locus_tag="OO590_003008"
     CDS             232..687
                     /locus_tag="OO590_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638300.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(765..1676)
                     /gene="rdgC"
                     /locus_tag="OO590_003009"
     CDS             complement(765..1676)
                     /gene="rdgC"
                     /locus_tag="OO590_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6638301.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1801..2709
                     /gene="mak"
                     /locus_tag="OO590_003010"
     CDS             1801..2709
                     /gene="mak"
                     /locus_tag="OO590_003010"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6638302.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2852..4033)
                     /gene="araJ"
                     /locus_tag="OO590_003011"
     CDS             complement(2852..4033)
                     /gene="araJ"
                     /locus_tag="OO590_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6638303.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(4159..7302)
                     /gene="sbcC"
                     /locus_tag="OO590_003012"
     CDS             complement(4159..7302)
                     /gene="sbcC"
                     /locus_tag="OO590_003012"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6638304.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7299..8501)
                     /gene="sbcD"
                     /locus_tag="OO590_003013"
     CDS             complement(7299..8501)
                     /gene="sbcD"
                     /locus_tag="OO590_003013"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6638305.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8691..9380
                     /gene="phoB"
                     /locus_tag="OO590_003014"
     CDS             8691..9380
                     /gene="phoB"
                     /locus_tag="OO590_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6638306.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9438..10733
                     /gene="phoR"
                     /locus_tag="OO590_003015"
     CDS             9438..10733
                     /gene="phoR"
                     /locus_tag="OO590_003015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6638307.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            11140..12459
                     /gene="brnQ"
                     /locus_tag="OO590_003016"
     CDS             11140..12459
                     /gene="brnQ"
                     /locus_tag="OO590_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6638308.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12535..13908
                     /gene="proY"
                     /locus_tag="OO590_003017"
     CDS             12535..13908
                     /gene="proY"
                     /locus_tag="OO590_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6638309.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            14064..>14843
                     /locus_tag="OO590_003018"
     CDS             14064..>14843
                     /locus_tag="OO590_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HCR6638310.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWF"
BASE COUNT         3282 a         3781 c         3997 g         3783 t
ORIGIN      
        1 cgcacatacc ccggcgtcgt tgttaacgac attgactgag cctcaggatc ggtcgctggc
       61 tattaataat ccacagctgg ctgccgatgt gaaaacggcg tggttaaaag aggatccatc
      121 attactctta tttgtcgaac aacccgatct ttcgcagtta cgtgatttag tgaaaaccgg
      181 ggcaacgcgg aaaattcgca gtgaagcgcg tcaccggctt gaggaaaaac aatgacacaa
      241 cgtccatgga gcaagttaca gcgggagatc tatgacttac tgacgccaac gatcaacctg
      301 caaattcact gtacccgtta tcccatgcgc agccaaaatg gtggaagtac cgatttaccg
      361 cgttattgga tcacgttgga taaaaacgtg atctgggatt acccgaaaga ctttattgca
      421 ggtaacgctg gagtgcgtaa ttttcacggt gaaacttgct ggtatcctta cctgacggat
      481 atttgttcaa tttctgattt attgcgtgaa tatattgata ccccaaaagc ggagctgtta
      541 acaaaacaat ttaccagcga taaatggggg ttagtgaata ttctgcgtgc tgctgacagg
      601 cgaataggga tgaggcgatt agatcagtta cggcgtaaaa cgcataatat tgctgcgcta
      661 aaaattattg ctcgccgtag cgaataatta tatgcctggt gtggcttcgt acgccggata
      721 agacgcggca ggcgtcgcat ccggcattaa aggaaaatca gcaattaacg ttgtgcttcg
      781 ccacctaatc cttcaatcag gttttgaatt aacgctgcca gttcaccagt catcaggatg
      841 aaatcggcat caaaacgctg ggcgaaatct tcacggtcga tatcttcgtt ttgatcgtgc
      901 agctcgtcgc agaacttaag acgcttgagc gaaccatcgt cgcacatcac aaactgaatg
      961 cgctgctgcc agtcgagcgc cagtttagtc accacttttc cggcttcaat gtgattggtg
     1021 atctcttcgc tggtcagatc ttgtttcttc gcgcggatca cgccgccatc ttccagcaac
     1081 gatttcagct cggcttcatc aagcagctgg aagccctgtg ccgcactacc ggagcgaacc
     1141 cattcggtca gcgtcagttc aatcgggttt tccatgctca atggtacaac cggtaacgac
     1201 cccaggcttt tacgcagtaa tgccagcgta tcttccgctt ttttggcact ggcgcagtcc
     1261 accataatca aaccgttaac cgtgtcgagc cacatcattg tctggctaaa acggctgaaa
     1321 gcacgcggca gcagagagtg cagcacttcg tctttcagcg aatctttttc ggttttcttc
     1381 agcttacgcg cctgttccgc ttccagtttg gcgattttcg cttccagcgc ctgtttaatc
     1441 accggagacg ggaggatttt ttcttctttg cgcgcgcaga taacaatttg accattggca
     1501 acgtgcgtta acgcatcgct gtgcgatccc atcggaggaa cccagcccat cttcgccatg
     1561 tcctggctgc cgcatggggt aaatgccatc gaggctagct gtttttccat ctcttctgca
     1621 cgcagcgaaa tctcgcggct aagacggtaa accattaaat ttttgaacca cagcatgata
     1681 atttccacgg ccttgtcgtt aaatttagcg ggcatgataa cgaattgtcg gcggccttgc
     1741 attgccaatc cggttgtccg tctctacgct attgatattg aaaaaaataa ggagagtaca
     1801 gtgcgtatag gtatcgattt aggcggcacc aaaactgaag tgattgcact gggcgatgca
     1861 ggggagcagt tgtaccgcca tcgtctgccc acgccgcgtg atgattaccg gcagactatt
     1921 gaaacgatcg ccacgttggt tgatatggcg gagcaggcga cagggcagcg cggaacggta
     1981 ggtatgggca ttccaggttc gatttcgccc tacaccggtg tggtgaagaa tgccaattca
     2041 acctggctca acggtcagcc attcgataaa gacttaagcg cgaggttgca gcgggaagtg
     2101 cggctggcaa atgatgccaa ctgtctggcg gtttcagaag cagtagatgg cgcggcagcg
     2161 ggagcgcaga cggtatttgc cgtgattatc ggcacgggat gcggcgcggg cgtggcattc
     2221 aatgggcgga cgcatatcgg cggcaatggc acggcaggtg agtggggaca caatccgcta
     2281 ccgtggatgg acgaagacga actgcgttat cgcgaggaag tcccttgtta ttgcggtaaa
     2341 caaggttgta ttgaaacctt tatttcgggc acgggattcg cgacggatta tcgtcgtttg
     2401 agcggacatg cgctgaaagg cagtgaaatt atccgcctgg ttgaagaaag cgatccggta
     2461 gcggaactgg cattgcgtcg ctacgagctg cggctggcaa aatcgctggc acatgtcgtg
     2521 aatattctcg atccggatgt gattgtcctg gggggcggga tgagcaatgt agaccgttta
     2581 tatcaaacgg ttgggcagtt gattaaacaa tttgtcttcg gcggcgaatg tgaaacgccg
     2641 gtgagtaagg cgaagcacgg tgattccagc ggcgtacgcg gcgctgcgtg gttatggcca
     2701 caagagtaaa aaacgtaggc aattggcgca tcatgcctga tgcgacgctt gccgcgtctt
     2761 atcaggccta caaaatgtgc cagaaccgcg tagggcggat aaggcgttca cgccgcatcc
     2821 ggcaataagc aacgcgcacc gtgacagtaa tctaccccag tggtttcgcc agcaccggac
     2881 tatccgccgc ttgctggcgc ttatagcgac catacagcag caacgatgac atcgcagcaa
     2941 acgaaaacag ggcggcaggc agtgccacgt aattatatgc cagccccagc gtcagcatca
     3001 tacctccgca atatgcgccg acggcgctac cgaggttaaa cgctatttgc ccacctgcgg
     3061 cacctaataa ctctccgcct ttggcatttt gcagtaacaa tatttgcaga ggtgctgaaa
     3121 gggcaaataa tcccgcgcaa caaataaaag caaaaataag cgacgttgtt ttcatgccgc
     3181 cgcagaaaaa gagcatcagc agtgccagta caatgataaa gtcagtcact gctgcaatgc
     3241 gcagtggtga ataacgtcct gaaatcctgc cacttagcat atttcccagc accatcccta
     3301 gcccaactaa catcataata aaggtcatcg ccgtttccga aaaaccggaa ataaacatca
     3361 tgtatggctt tacgtagctg aaccaggcaa acacacctgc gttgccaaac atcgtggcgg
     3421 cgaaaattaa ccacggggcc gggctgcgca aaaagtgaaa ttgttcgcgc agatttcctt
     3481 tcgcctcgtc gcgaatatct ggcacccaaa aatagaccga tgccatcacc gcaatattaa
     3541 aaacagcgat caataaaaag gtgtaacgcc agcattcctg acttaaatac gttcccagcg
     3601 gaatgcccag caaattggcg actgtcatcc cggaaaccat ccccgccacg gcggcggtga
     3661 cttttccggg tttgataatt tttgataaca cgatcgctcc gacgccaaaa aatgcgccat
     3721 gcggaaagcc ggataccagc cgaccaatgg cgagcatcag gtaagacgaa gagagcgtga
     3781 acatggcgtt gccaatgacg cacaacgcca ccagaaacaa caagatatgt ttgagtgagt
     3841 agcggctgga aaagagtgcg atgattggcg caccgaccac caccaccagt gcataatacg
     3901 agatcatatg cccggcagca ggaatcgaaa ttcctacgtt atgcgccagc tccgtgagca
     3961 cgctcataat gccaaattcg gctatcccca aaccaaacgt gcccagagcc aaagataaaa
     4021 tgactttttt cataccacca tcacaaccac cagaacggct accaatcacc gtagccaata
     4081 aagtgatagc ttgcaggata acgaccagca aggccaagag ataattcgcc cctctgtatt
     4141 cattatcctg ctgaatagtt atttcactgc aaacgtactt tccagtttgc tgtagcccag
     4201 gccgttgatc tttttcactt tgatctgcac cggaatacgc tctttcatcg cttctacgtg
     4261 gctaatcaca ccgatggttt tgccactggc gttcagggca tccagcgcat caagggcggt
     4321 atccagcgtt tcgctatcca gcgtgccaaa accttcatca aggaacagcg agtcaatacg
     4381 tgttttatgg ctgaccagat ccgaaagcgc cagcgccagc gccagactaa cgaggaaact
     4441 ttcgccgccg gaaagggtac gggtatcgcg taccgcatct gcctgccagg tatcaacaac
     4501 ctcgacttcc agcgcctcgc tggctttgcg ctgtaacaga tagcgcccgt gcagccgggt
     4561 aagttgctga ttagcgagat ggactaaatt atccagcgtc agcccctggg caaacttgcg
     4621 gaatttatcg ccctctttgg aacctattag cgaattcaga tatccccagt cctcaacctg
     4681 ctgcgtcatt tgagcaattt gctgcattaa ggtttgttgt tgctgacggt tatctgcatc
     4741 ctgcttcagc tgctggcgaa tctcgccttg actcgtggtg ttttcacgca acttttggtg
     4801 agtttgcgct aactcttgct gaatctgctc caccgtcaca gtgagagcca acccgtcagg
     4861 tcggtgttgt tgatgctgtg ccagcgtttc tgctgtctga gtgaccagcg tttgcgcctg
     4921 acggcgctgg ttttccagat tctgcttgag ctgttccagc tgcgttagtg tttgttcatc
     4981 cattagcgcc gcaaggaacg cctgctgatc gtcaaagacg ctggcctgta gcgtggtgtc
     5041 aaactgcgct tgggcttttt gcagactttg cgccgccaga acatcctgtt gctgtaacgt
     5101 ctgctgctgg ctgtgtaatg cgagacattg ttcatgaacc tgccgccagt tatccagcgc
     5161 cacagtttct tcgctgtgcg ggagatcatc actttgcggc aacgtttcca gaatcggcgt
     5221 cagctgctga atacggtttt gcagcgcggt taattcgttc tggcgttgct gccagctctg
     5281 cgcttcttgc tgacgtgtcg ccaaccagct ctcttcttca tcttcctgtg gcaatgtcag
     5341 tgcataaccc gccaatgccg ttaagagctg ctgctgacgt tgttcaattt gctgttgata
     5401 ctggataatt tgctgattat gcgcggcaat ctgcccttgt aattcatgtc gttggctgag
     5461 taaccgcagc tggcgttcgt gctcatcttg tgcatccagc cacggttgaa tatcgtcctg
     5521 tggctgcaag gtgatattga ggctggccgt gacggcttgc cattgttgag taagtgcttg
     5581 ctcatcttgt cggaggcttt gcgcttcgtt ttcatcgcgc tgaagctgct tcgttaatgc
     5641 atccagttgc ccgcgcagcg ccgcaccttc ttcgccgagc tttttaactt cgttttccag
     5701 cgccagtaat cgagactgat taacaccagg ctccagcgcc tgatacgcct cgaccgccgg
     5761 gtggctggtg gaaccacaaa gtggacaagg ctgacccgcc tgtaactgtg cacgctgggc
     5821 ttccagcgtt ttgatgcgcg cttcctgctc gcaaatggtt ttcacatcgg caagttgctg
     5881 cgtcttttct ttataacgct ggcgcatttc gttaagtgcg acattacgtt gcgtctgttc
     5941 cagagtgaca ttctggatag tgaccattaa ctgcgccaga cgtttttgtt ggggaacaat
     6001 ctgcccatgc agcgcgacca ggcgctgacg cagtgggcgt tgctcggcat gttgcgccag
     6061 ggcggtagca acttcatcgg cggttaacgt caacgtgatc gccgcaagcg cattaagttt
     6121 ttgctcagca tgggttaact gttgctgcca ttgccgcaga tgctcgcgat cgctggtttg
     6181 ttgggagaac tgcgcacgcc aacccgccag ttcgttgttc cactgacgga agcggtcgtg
     6241 ttcctgtaac caggtattca ggctttgttg ctgctgctgt aattctgctg actgcttcgc
     6301 cgcgtggtgg cgaatgctcg cgcgaagcgc cattgtgctc tgtaagcgag tatttacttc
     6361 ttcaatctgc tggcgaatat gcgccagcgc cgcgctgtgt tctgcgatgc gttcccagtg
     6421 tggacgaaga tttcgtgccg gttgtgccag actaagcgcc gccagttgag gttgcgcttt
     6481 ttcttcttcg gctaacgcct gttgcaaggc ctgctgacgg cggctggctt cttgctgcaa
     6541 ttcgtcctga cgcgttaacc agtttagcga ttgttgttct tgctgctgcg cggtaattaa
     6601 ctgtttttct tcgtcagtaa gtacctgcaa actcgctgtc agcgattgca cttgttccgg
     6661 cgtgagcaac gtgacgccgc tggcctgcgc ttgcagcttc tccagctcag tacgggccga
     6721 tttgtgctgc tcaaaaacca tcgccgagat ttgcccgtag atttcagtgc cggttaactc
     6781 ctcgagcaat tccgcgcgtt ctttgggttt ggcattcagg aaggcagcaa attgcccctg
     6841 cgaaagcagc atcgaacggg tgaagcgccc gtaatccagc ccggttaacg tcgctgtcag
     6901 ttccagctta tctttcactt tgtcggcgag aattttgccg tcggcgcagc gcgccagctc
     6961 tacgcgaggc acctgcaaat taccgtccgg ctggttacgc gcccgattct ggctccagaa
     7021 cgcgcgatac gcttcacctt tcacttcaaa ctccacctcc gccagacatt cagcggtatc
     7081 gcgggtcatg agatcatttt gcgattgtga aacgttagag agacgcggag tttcgtgata
     7141 cagcgccaga caaatggcgt ccagcagggt ggttttcccc gcaccggttg ggccggtaat
     7201 ggcaaacagc ccgttgctgg cgaacggctc gcgggtgaaa tcaatcttcc attcgccttt
     7261 taatgagttc aggtttttca ggcgcaggct gagaattttc atgcttcgtg ttctccggcg
     7321 agggtatgca acgtcgtggt gaaaagatgc tgcagacgtt gctgctgcga ttcatccagt
     7381 tcttccagtg ccagacggcg attgaacacc tcttcgacgc tgagttcgct gagggtttca
     7441 cgctgttggc tggctaacac acgctcgcgc tgttcacgac tccgacgtac cagcaatact
     7501 tcgacaggca atgattcggt taatgcctgg attttgcgct gaatatcatg cagatactca
     7561 tcagtagtga tttcgatatc cagccagaca ggtggctcct gcgatacatc gcgccactgt
     7621 tccagctgtg cggtaatcga cgccagatcg cctttcagca ccgccatggg ttgagttacc
     7681 ggtacgttca ggttttccac gctctctaat ttgccgtttg aaaatgtcac cagatggaca
     7741 tatttactct taccgcattc atcaaaactc agtggaatgg gggaaccgca atagcgaaca
     7801 tgttccatgc cgccaataat ctgtgcgcgg tgaatatgcc cgagcgcgat gtagtcggct
     7861 ggtggaaagt tttgtgccgg aaacgcgtcc agcgtgccaa tataaatgtc acgcacggcg
     7921 tcacttttac tggcacccac ggtcgttaaa tgtcccgtgg cgatgatggg cagaggctga
     7981 tcgccgcgca gtttgcaggc atcggcatag tgttgttggt aataatcggt aatcgctgcc
     8041 agtaaatgct gctgtttttc aataccgtta agccccgcct ggctggaaat aatgtcacgc
     8101 gggcgtaaaa acggaatggg gcacagcact gcgcctggcg tcccgtcgcg acgaggcaag
     8161 atttgcggcg catgtccggc gctggcgacc acggttgtat tgaggaacgc catgatatcg
     8221 cgcgattcat tcagcgtggc gaccgagtca tggtttcctg ccagtaccac cagatgacag
     8281 ccagtttgct gtaaattgac aacaaaacgg ttgtataacg tgcgggcgta actgggcggc
     8341 gagccggtat cgaaaacatc accagcaaca ataatcgcat ccacctgatg ggtttgtgct
     8401 gtctccagca gccagtcaag aaaagcctga tgttcagctt cgcggctttt actgtagaag
     8461 ttctggccga gatgccagtc tgaggtgtga aggatgcgca taacggttcc ctggcgaaaa
     8521 agcatggtcg cgattatacc caaacagatg tgccatttgc ttttttctgc gccacggaaa
     8581 tcaataacct gaagatatgt gcgacgagct tttcataaat ctgtcataaa tctgacgcat
     8641 aatgacgtcg cattaatgat cgcaacctat ttattacaac agggcaaatc atggcgagac
     8701 gtattctggt cgtagaagat gaagctccaa ttcgcgaaat ggtctgcttc gtgctcgaac
     8761 aaaatggctt tcagccggtc gaagcggaag attatgacag tgctgtgaat caactgaatg
     8821 aaccctggcc ggatttaatt ctccttgact ggatgttact tggcggctcc ggtatccagt
     8881 tcatcaaaca cctcaagcgc gagtcgatga cccgggatat tccagtggtg atgttgaccg
     8941 ccagagggga agaagaagat cgcgtgcgcg gccttgaaac cggcgcggat gactatatca
     9001 ccaagccatt ttcgccgaag gagctggtgg cgcgaatcaa agcggtaatg cgccgtattt
     9061 cgccaatggc ggtggaagag gtgattgaga tgcagggatt aagtctcgac ccgacatctc
     9121 accgagtgat ggcgggcgaa gagccgctgg agatggggcc gacagaattt aaactgctgc
     9181 acttttttat gacgcatcct gagcgcgtgt acagccgcga gcagctgtta aaccacgtct
     9241 ggggaactaa cgtgtatgtg gaagaccgca cggtcgatgt ccacattcgt cgcctgcgta
     9301 aagcactgga gcccggcggg catgaccgca tggtgcagac cgtgcgcggt acaggatatc
     9361 gtttttcaac ccgcttttaa cgccttgctc atcggacgca gagcagggct tatgatttct
     9421 taactggagt atcttacgtg ctggaacggc tgtcgtggaa aaggctggtg ctggagctgc
     9481 tactttgctg cctcccggct ttcatcctgg gtgcattttt tggttacctg ccctggtttt
     9541 tgctggcatc ggtaacagga ctgcttatct ggcatttctg gaatttattg cgcctttcat
     9601 ggtggctgtg ggtggatcgc agtatgaccc cgccaccggg gcgtggtagc tgggaaccgc
     9661 tactatacgg cttacaccag atgcagctgc gaaataaaaa acgccgccgt gaactgggca
     9721 atctgattaa acgctttcgt agcggcgcgg agtcgctgcc cgacgcggtg gtgctgacca
     9781 cggaagaggg cggtattttc tggtgtaacg gtctggcgca acaaattctt ggtttgcgct
     9841 ggccggaaga taacgggcag aacatcctta acctactgcg ttacccggag tttacgcaat
     9901 atctgaaaac gcgtgatttt tctcgcccgc tcaatctggt gctcaacacc gggcggcatc
     9961 tggaaattcg cgtcatgcct tatacccaca aacagttgtt gatggtggcg cgtgatgtca
    10021 cgcaaatgca tcaactggaa ggggcgcggc gtaacttttt tgccaacgtg agccatgagt
    10081 tacgtacgcc attgaccgtg ttacagggtt acctggagat gatggatgag cagccgctgg
    10141 aaggcgcggt acgcgaaaaa gcgttgcaca ccatgcgcga gcagacccag cggatggaag
    10201 ggctggtgaa gcagttgcta acgctgtcga aaattgaagc ctcgccgacg caattgctca
    10261 atgaaaaggt tgatgtgccg atgatgctgc gcgttgttga gcgcgaggct cagactctga
    10321 gtcagaaaaa acagacattt acctttgaga tagataacgg cctcaaggtg tctggcaacg
    10381 aagatcagct acgcagtgcg atttcgaacc tggtctataa cgccgtgaat catacgccgg
    10441 aaggcacgca tatcaccgta cgctggcagc gagtgccgca cggtgccgaa tttagcgttg
    10501 aagataacgg accgggcatt gcaccggagc atattccgcg cctgaccgag cgtttttatc
    10561 gcgttgataa agcgcgttcc cggcaaaccg gcggtagcgg attagggtta gcgatcgtga
    10621 aacatgcggt gaatcatcac gaaagtcgcc tgaatattga gagtacagta ggaaaaggaa
    10681 cacgtttcag ttttgttatc ccggaacgtt taattgccaa aaacagcgat taatccgcct
    10741 ttgtcatctt ttattgccat aagccagccg atgctggctt attttctttg cagtcaaaat
    10801 acgggcgtta gattttacaa cggttggtga ttttttgttc gcatgattag ccatgtcttt
    10861 ttcacggaaa tagtgtttta tactggttgg tgatttctta tcgctatata cctctggttt
    10921 ttagatccct ccttgcttta aaacgttata agcgtttaaa ttgcgcttca ggtgctgtca
    10981 tactgactgc attaacgcgg taaatcgaaa aactattctt cgccgcgcct ggttgggagt
    11041 attttccgct aaaattgttt aaatataccg ctgtatcatc cccagggatt ggcacaaaaa
    11101 tttaacgtta caacaccaca tccacaggca gtatgattta tgacccatca attaagatcg
    11161 cgcgatatca tcgctctggg ctttatgaca tttgcgttgt tcgtcggcgc aggtaacatt
    11221 attttccctc caatggtcgg cttacaggca ggcgaacacg tctggactgc ggcattcggc
    11281 ttcctcatta ctgccgttgg cctgccggta ttaacggtag tggcgctggc aaaagttggc
    11341 ggcggtgttg acagcctcag cacgccaatc ggtaaagtcg ctggcgtact gctggcaacg
    11401 gtttgttacc tggcggtggg gccgcttttc gctacgccgc gtacagctac cgtttccttt
    11461 gaagtgggga ttgcgccgct gacgggtgat tccgcgctgc cgctgtttat ctacagcctg
    11521 gtctatttcg ctatcgttat tctggtttcg ctctatccgg gcaagctgct ggataccgtg
    11581 ggcaacttcc ttgcgccgct gaaaattatc gcgctggtca tcctgtctgt tgtcgcgatt
    11641 gtctggccgg cgggttctat cagcacggcg actgaggctt atcaaaacgc tgcattttct
    11701 aacggcttcg ttaacggcta tctgaccatg gatacgctgg gcgcaatggt gtttggtatc
    11761 gttattgtta acgcggcgcg ttctcgtggc gttaccgaag cgcgtctgct gacccgttat
    11821 accgtctggg ctggcctgat ggcgggtgtt ggtctgactt tgctgtacct ggcgctgttc
    11881 tgtctgggtt cagacagcgc gtcgctggtc gatcagtctg caaacggcgc tgctattctg
    11941 catgcttacg ttcagcacac ctttggcggc ggcggtagct tcctgctggc ggcgttaatc
    12001 ttcatcgcct gtctggtcac ggcggttggc ctgacctgtg cttgtgcaga attctttgcc
    12061 cagtacgtac cgctctctta tcgtacgctg gtgtttatcc tcggcggctt ctcgatggtg
    12121 gtgtctaacc tcggcttaag ccagctgatc cagatctccg taccggtgct gaccgctatt
    12181 tatccgccgt gtatcgcact ggttgtatta agttttacac gctcatggtg gcataattcg
    12241 tcccgcgtga ttgctccgcc gatgtttatc agcctgcttt ttggtattct cgacgggatc
    12301 aaagcatctg cattcagcga tatcttaccg tcctgggcgc agcgtttacc gctggccgaa
    12361 caaggtctgg cgtggttaat gccaacagtg gtgatggtgg ttctggccat tatctgggat
    12421 cgcgcggcag gtcgtcaggt gacctccagc gctcactaaa tcactgaaca tttgttttaa
    12481 ccacggggct gcgatgcccc gtggtttttt attgtgttga tgggttagga attgatggaa
    12541 agtaagaata agctaaagcg tgggctaagt acccgccaca tacgctttat ggcactgggt
    12601 tcagcaattg gcaccgggct gttttacggt tcggcagacg ccatcaaaat ggccggtccg
    12661 agcgtgttgt tggcctatat tatcggtggt atcgcggcgt atatcattat gcgtgcgctg
    12721 ggggaaatgt cggtacataa cccggccgcc agctctttct cgcgttatgc gcaggaaaac
    12781 cttggcccgc tggcaggtta cattaccgga tggacctact gctttgaaat ccttattgtc
    12841 gccatcgccg atgtgaccgc ttttggtatc tatatggggg tcttgttccc gacggtgccg
    12901 cactggattt gggtactgag cgtggtgctg atcatttgcg ccgtaaacct gatgagcgtg
    12961 aaggtcttcg gtgagctgga attctggttc tcgttcttta aagtcgccac catcatcatc
    13021 atgattgtcg ccggtttcgg catcatcatc tgggggattg gcaacggcgg gcaaccgacc
    13081 ggtattcata acctgtggag caacggcggc ttcttcagta acggctggct tggcatggta
    13141 atgtcgttgc aaatggtgat gtttgcttac ggtgggatcg aaattatcgg gattaccgcc
    13201 ggtgaagcga aagatcctga gaaatcgatt ccgcgtgcga ttaactccgt gccgatgcgt
    13261 attctggtgt tctacgtcgg tacgctgttc gtcattatgt ctatctaccc gtggaatcag
    13321 gttggcactg ccggtagccc gttcgtgctg acgttccagc atatgggcat tacctttgcc
    13381 gccagcattc ttaactttgt tgtgctgacc gcttcgctgt cggcaattaa cagtgacgta
    13441 tttggcgtag gccgtatgct ccacggtatg gcagagcagg gcagcgcacc gaaaattttc
    13501 agcaaaacgt cgcgtcgcgg tattccgtgg gttacggtgc tggtgatgac taccgcgctg
    13561 ctgtttgcgg tgtatctgaa ctacatcatg ccggaaaacg tcttcctggt gatcgcttcg
    13621 ctggcaacct tcgccacggt atgggtgtgg attatgatcc tgctgtcgca aatcgccttc
    13681 cgtcgccgtt tgccgccaga agaagttaag gcgctgaaat ttaaagtgcc gggtggggta
    13741 gcaacgacca tcggcgggct gattttcctg ctctttatta tcgggttgat tggttatcac
    13801 ccggatacgc gtatctcgct gtatgtcggt ttcgcgtgga ttgttgtgct gttgattggc
    13861 tggatgttta aacgccgcca cgatcgtcag ctggctgaaa accaataata cttgcgttct
    13921 gtaagccggt tcaggaggaa atcctgatcc ggctattccg aaagttatcc gcccccgtcc
    13981 tcctccccca aatatccttc agatgatgag tgatcctgca ttaggctatg gcaaggtgat
    14041 cagattttca tcacagggga attatgatgt taaatgcatg gcacctgccg gtgcccccat
    14101 ttgttaaaca aagcaaagat caactgctca ttacactgtg gctgacgggc gaagacccac
    14161 cgcagcgcat tatgctgcgt acagaacacg ataacgaaga aatgtcagta ccaatgcata
    14221 agcagcgcag tcagccgcag ccaggcgtca ccgcatggcg tgcggcgatt gatctctcca
    14281 gcggacaacc ccggcggcgt tacagtttca aactgctgtg gcacgatcgc cagcgttggt
    14341 ttacaccgca gggcttcagc cgaatgccgc cggcacgact ggagcagttt gccgtcgatg
    14401 taccggatat cggcccacaa tgggctgcgg atcagatttt ttatcagatc ttccctgatc
    14461 gttttgcgcg tagtcttcct cgtgaagctg aacaggatca tgtctattac catcatgcag
    14521 ccggacaaga gatcatcttg cgtgactggg atgaaccggt cacggcgcag gcgggcggat
    14581 caacgttcta tggcggcgat ctggacggga taagcgaaaa actgccgtat ctgaaaaagc
    14641 ttggcgtgac agcgctgtat ctcaatccgg tgtttaaagc tcccagcgta cataaatacg
    14701 ataccgagga ttatcgccat gtcgatccgc agtttggcgg tgatggggcg ttgctgcgtt
    14761 tgcgacacaa tacgcagcag ctgggaatgc ggctggtgct ggacggcgtg tttaaccaca
    14821 gtggcgattc ccatgcctgg ttt
//
LOCUS       DAJTYF010000096        14834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTYF010000096 DAJTYF010000000
VERSION     DAJTYF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.096"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            39..1244
                     /locus_tag="OO590_003019"
     CDS             39..1244
                     /locus_tag="OO590_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR6638311.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            1294..1494
                     /locus_tag="OO590_003020"
     CDS             1294..1494
                     /locus_tag="OO590_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638312.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            1497..1820
                     /locus_tag="OO590_003021"
     CDS             1497..1820
                     /locus_tag="OO590_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR6638313.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            1817..2227
                     /locus_tag="OO590_003022"
     CDS             1817..2227
                     /locus_tag="OO590_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6638314.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRLRVDMPADNFGVEPQYL
                     ITFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECGQSHGGNNDDFLFAR"
     gene            2202..2708
                     /locus_tag="OO590_003023"
     CDS             2202..2708
                     /locus_tag="OO590_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638315.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            2705..3265
                     /locus_tag="OO590_003024"
     CDS             2705..3265
                     /locus_tag="OO590_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638316.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            3274..3441
                     /locus_tag="OO590_003025"
     CDS             3274..3441
                     /locus_tag="OO590_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6638317.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNEKVNTDDEL"
     gene            3428..4921
                     /locus_tag="OO590_003026"
     CDS             3428..4921
                     /locus_tag="OO590_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6638318.1"
                     /translation="MTSFNTIPSNTLVPIFYAEMDNSAANTAQDSGASLLIGHANNGA
                     EIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATVT
                     LTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPALPFTASSSAG
                     VVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAAM
                     ADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAGD
                     QFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKGK
                     RFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAYV
                     LRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYEL
                     FKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            4921..5277
                     /locus_tag="OO590_003027"
     CDS             4921..5277
                     /locus_tag="OO590_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6638319.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPASKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            5277..5546
                     /locus_tag="OO590_003028"
     CDS             5277..5546
                     /locus_tag="OO590_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6638320.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            5688..7523
                     /locus_tag="OO590_003029"
     CDS             5688..7523
                     /locus_tag="OO590_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6638321.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETISPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYIPYPAVDLAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            7584..8912
                     /locus_tag="OO590_003030"
     CDS             7584..8912
                     /locus_tag="OO590_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR6638322.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEENAGTGRRVETHEYPNRDKPYTE
                     DLGKVTFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKGEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDVARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVEQATTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            8909..9988
                     /locus_tag="OO590_003031"
     CDS             8909..9988
                     /locus_tag="OO590_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR6638323.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEISRQWPGD
                     GGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRELVQIAQALAAPFGIEVVNNGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEDPGARKKKKARVQEDPF"
     gene            9988..10536
                     /locus_tag="OO590_003032"
     CDS             9988..10536
                     /locus_tag="OO590_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR6638324.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCLTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            10536..10961
                     /locus_tag="OO590_003033"
     CDS             10536..10961
                     /locus_tag="OO590_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6638325.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYASRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            10948..12006
                     /locus_tag="OO590_003034"
     CDS             10948..12006
                     /locus_tag="OO590_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6638326.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDEFWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRLPIACSTAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWAY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGADGEYSHQLLAPVD
                     NISIAKNELAVLGTISWT"
     gene            11997..12581
                     /locus_tag="OO590_003035"
     CDS             11997..12581
                     /locus_tag="OO590_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR6638327.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSSSDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGIQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRQDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNSTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            12585..13235
                     /locus_tag="OO590_003036"
     CDS             12585..13235
                     /locus_tag="OO590_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638328.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            13207..13641
                     /locus_tag="OO590_003037"
     CDS             13207..13641
                     /locus_tag="OO590_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HCR6638329.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYLTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(14414..>14834)
                     /locus_tag="OO590_003038"
     CDS             complement(14414..>14834)
                     /locus_tag="OO590_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6638330.1"
                     /translation="FGFIVFSITAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAI
                     NTIITTVGLIIFNIFIAKIICGNDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSV
                     TIPNPICAIIYTCIYVYVSDKIGHWLSRTVGGPFLIK"
BASE COUNT         3568 a         3600 c         4330 g         3336 t
ORIGIN      
        1 gcactgaaaa atctgaactt ctaatcagga gaaatacgat ggcggtagat attaaagatg
       61 tcgaacaggt cgcgcaggag ctgcagcaga agtttgacga cttcaaagca aagaacgaca
      121 agcgcgtgga tgcgattgag caggaaaaag gcaagcttgc cgggcaggtg gaaaccctga
      181 acgggaaact cagcgagctg gaaaatctca aaagcgatct tgaaaaagag ctgcttgagc
      241 tgaaacgtcc ggcaggtggc gcgcaaaata aactggccac cgagcataaa gaagcgtttg
      301 tgggcttttt gcgtaaaggc cgtgaagatg gtctgcgcga tctggagcgt aaggcattgc
      361 aggtgggcac cgatgaagac ggtggctatg ccgtgccgga agcgctggat cgcaacattc
      421 tcaccctgct gaaagatgaa gtggtgatgc gccaggaagc cacggtgatc accgttggcg
      481 gttccgacta caaaaaactg gtgaatctgg gcggcacggc ttccggatgg gtgggggaaa
      541 cggatacgcg atcccagact gccacctcca gactggagct gattgaacct ctcatggggg
      601 aaatctacgg caacccgcag gccacccaga aaatgctgga cgatgccttc ttcaacgtgg
      661 aggcctggat caacagcgag ctggcaaccg aatttgccga acaggaagaa attgccttta
      721 ccaccggcga tggtaccaag aagccgaaag ggttcctggc gtatgaatcc actgatgaaa
      781 ccgataaggt ccgggcgttc ggcaaacttc agcatattgt atccggcgaa gcgacggcgg
      841 tgaccgcaga cgccattatc aaactgattt acacgctgcg taaggcacac cgcaccggcg
      901 cgaagttcat gatgaacaac aacagcctgt ttgccatccg tctgcttaaa gacagcgagg
      961 gtaactatct gtggcgtccg gggctggaac tggggcagcc gtcctctctg gcgggttacg
     1021 gtatcgctga aaacgaacag atgccggata tcgccgctga tgcgaaagcc attgcatttg
     1081 gtaacttcaa acggggttac accatcgttg accgtatcgg cacccgcatt ctgcgtgacc
     1141 cgtacaccaa taaaccgttt gtcggttttt ataccaccaa gcgcaccggc gggatgctgg
     1201 tcgattcgca ggccatcaaa ctgctgaaga ttgcagcggc gtaatcactc aggggcgcgg
     1261 aaccgcgccc cctgttctga cgggtgaaga atcatgatcc tgaaacaaga tctgaaatgg
     1321 tcaccggacg gtatgcgtgt tgaggtcatt cgggccggtg agtatgacga cggggcgctt
     1381 cctgcccggg tgcaggagat tgcacttcag gccgggttag cagagcgcgg aaccagtgca
     1441 aaaagcagta aagcgacaaa agagaaaaaa gccacgacca gtaaagaggg ctgagtatgc
     1501 ttctgacaat ggaagagatt aaagcccaac tccggctgga tgaggatttc gatgctgatg
     1561 accgccatct gcaactgctg gcctgtgcgg cacaaaagcg gacggaaacg tatctgaacc
     1621 ggaagctcta tgcaccggat gaaaccattc cggacagcga tccggacggg ctgcacctgc
     1681 cggatgatat tcgtctgggg atgctgatgc ttatcagcca tttttacgaa aaccgctcgt
     1741 cggttacgga agtggagaaa cttgacatgc cgcagagttt tggctggctt gtcggcccgt
     1801 acaggtactt tccgcaatga aaattcgtca ggcgcagacc agcgcaacct acattctgcc
     1861 ggaccccggt gaactgaata aacgcgtcct gatccgcctg cgggtggata tgcccgcgga
     1921 taactttggc gtggagcctc aatacctgat tacgttccgg acatgggcga aggttatcca
     1981 gaccagtgcc accacctggc aggaaaccgc gcagaccggg gacgccatca cccattacat
     2041 caccattcgt taccgccggg ggattaccgc tgattatgag gtggtctgcg gtgacagtgt
     2101 gtaccgggtg aaacgtcagc gcgatctgaa cggggcgcgg cgctttctgc tgctggagtg
     2161 tacggaactg ggcgaatgtg ggcagagtca cggaggcaac aatgacgact tcctttttgc
     2221 acgttgattt tcagcagccc gcggagatgc gctttaaccg cgcccgtgtc cggcgggcgt
     2281 ttgtcacgat tggtcagcgt catatgcgtg atgcccgtcg gctggtgatg cgccgtgcgc
     2341 ggtcggcacc gggtgaaaac cccggttatc agaccggacg cctggctcgt tcgattggtt
     2401 acatggtacc cagagccagt aaacatcgcc ctggttttat ggcacgtata gcccctaacc
     2461 agcgtaatgg agagggaaac cgccgtatca ccggtgattt ttatccggct tttttgttct
     2521 atggcgtgag gcgaggggca aagcgtcgtc gcagccatca tcgtggtgca tccggtggca
     2581 gcggctggcg actggctcca cgtaataact ttatggtgga aacgcttgaa aagaaccgca
     2641 gctggacacg ctattttctg gcgcgggaat tgcgtaaatc actgaagccg gagcgacgac
     2701 acagatgaaa ctgacgcctg ttattgctgc gctgcgtgcc cgctgcccgt attttgaaaa
     2761 ccgggtggca ggcgcggcac agttcaaaaa tctgccggag gtcggaaagc tgagactccc
     2821 ggcggcgtat gtggtaccgg gtgatgactc tccgggagaa aacaaaagcc agaccgacta
     2881 ctggcaggag ctgaaagagg gcttctccgt ggttgtcata ctgagtaacg ggcgtgatga
     2941 gcgcggtcag tttgcctcgt atgatgtggt ggacgatgtc cggcaaatgc tctttaaggc
     3001 cctgctgggc tggaacccgg aagcgtgcgg taacccgatt acctatgacg gcggcacgct
     3061 gctggatctg aatcgtcatg agctgattta tcagttcgat ttttcggtca tcagcgagtt
     3121 gaccgaagac gatacccgcc agcaggatga cctgaacagt ctggatgaac tgcgaacgct
     3181 ggcgattgat gttgattatc tagagcccgg taacgggcct gacggcgata tcgaacatca
     3241 caccgaaata acccttcctt cctgagaatc ttcatgtttg tgaaacctgt taaagggcgg
     3301 tcagttcctg accctgcccg cggagacctt ttgcccgccg aggggcgaaa tgttgatgag
     3361 aacaactact ggctgcgccg tgaagcagcg ggtgatatcc ggcgcgtgaa tgaaaaggtg
     3421 aataccgatg acgagcttta acaccattcc gtcaaatacg ctggttccga ttttttatgc
     3481 ggaaatggat aactcggcgg cgaatactgc acaggacagc ggagcatcgc tgctgattgg
     3541 tcatgccaat aacggtgcag agattgttgc caacagtctg gtgctgatgc catcggcaga
     3601 ctatgcacgc cagatttgtg gtgcgggaag tcagctggcg cgtatggtcg aggcttatcg
     3661 ccagaccgac ccgtttggtg agctgtatgt gattgccgtt ccagaagcca caggcgcggc
     3721 ggcaacggtt acgctgacgg tgaccgggga agcaaccgaa agcggcacgg tgaatgtcta
     3781 tgtgggacgt acccgcgtgc aggctccggt gaccaacggc gataacgtca cgacgattgc
     3841 cagcagtatc caggatgcca tcaatgccgt tccggccctg ccgtttacgg cttcatcttc
     3901 ggcaggcgtg gtcacactga ccgcgcgtca taaggggctt tgcgggaatg aaattcctgt
     3961 cagcctcaat tactacggct ttggtggggg cgaagtgctg ccagcgggcg tacagattgc
     4021 cgtggcgacg ggtaccgccg gaacgggtgc tccggttctc accggcgcgg tggctgcaat
     4081 ggcggatgag ccgtttgatt atatcggcct gccgttcaac gacacggcct ccgttaacac
     4141 gctggtgacc gagatgaacg ataccagcgg tcgctggagc tatgcgcgtc agctgtatgg
     4201 tcatgtgtat acggcaaaga tcggcacgct gtcagaactg gtgaccgcag gtgaccagtt
     4261 taaccagcag cacattaccc tggcgggata cgaaaaagag acccagacgc ctgccgacga
     4321 gctggcggca agccgtaccg cccgcgcagc ggtgtttatt cgcaacgatc cggcacgtcc
     4381 cacgcagacc ggtgagctgg tgggtatgct gcctgcgccg aaggggaaac ggttcacgat
     4441 gaccgaacaa cagaccctgc tgtctcatgg cgtggcaacg gcgtatgtcg aaagcggggt
     4501 gctgcgcatt cagcgtgatg tcaccacgta caggaaaaac gcttacggtg ttgcggataa
     4561 cagctacctc gacagcgaga cgctgcatac cagcgcgtat gtgctgcgca aactgaaatc
     4621 cgtcattacc agtaagtacg ggcgtcacaa gcttgccagc gacggtaccc gctttggtcc
     4681 cggtcaggcg attgtcacac cggcggtgat caaaggggaa ctgctggcaa cctaccgtca
     4741 gcttgagcgt gcggggatcg tggaaaacta cgaactgttt aagcagtacc tggttgtgga
     4801 gcgtgatgcc agcgatccga accgcctgaa cacgctgttc ccgcctgact atgttaacca
     4861 gttgcgtgtc tttgccgtgg ttaaccagtt ccgtcttcag tattcagagg agtctgcata
     4921 atggcccgta tcgggggaac ctgttatttc aaaattgacg gtcaacagct atcgctgacc
     4981 ggcggcattg aggtgcccat gaacaggacg gtcaatgatg acatcatcgg cctggacggt
     5041 tcagtggacc gcaaggaaac tcaccgtgcg ccttatgtca aagggacctt caaggtgccg
     5101 aagaattttc cggcgagcaa aatcacctcg tctgatgaga tgaccatcac tgccgagctg
     5161 gcgaacggtc aggtctatgt actgtcgtct gcctggctgc acggcgaagc gaaccataat
     5221 gccgaagaag gcacggtcga tcttgagttc cacggtgaag aaggggatta ccagtaatga
     5281 aagagcttga gttaaagaaa ccgattaccg ctcatggcga gacactctcc gtactggagt
     5341 ttgatgagcc caccgggaaa gatgtccgcg agctggggta tccctaccag atgaatcagg
     5401 atgagtccgt cagacttctg gcgcatgtgg tatcgaaata tattgtgcgg ctggcgaaag
     5461 tgccgcaaag ctctgtcgac cagatgtctc cggcagacct gaatgcagcg gcgtggcttg
     5521 tggctggttt tttcctccag gcctgacggc tgaatacctt actgatcgct tctttgattg
     5581 cgccagctac tggcgcatta atcccttcga attgctgaat atgccgatca gtgaaattcc
     5641 cttgctggtc agtcaggcaa acaggataga gcaggagaaa cgcacacatg gctgaatttg
     5701 agcttaaggc gttgatcacc ggtgtcgaca ggctttctcc cgcgctgtcg aaaatgcaaa
     5761 agaaaatccg gggatttaaa cgccaggcgg aagaagcgtc acagggtggg ctggcgcttg
     5821 gtggcggact ggcagcgggt ctgacgcttt ccctgaaatc ttatgccgat caggaaaacg
     5881 ccgccaccgg gctgaaagtc gccatgatgg atgcgaatgg cgaggttgga aagagctttc
     5941 aggacatcaa taaactggct attggcctgg gtaaccagct acccggtaca acggctgatt
     6001 tccagaacat gatgcagatg ctggtgcgtc aggggatccc ggcagaaaat attcttggcg
     6061 gtgtgggtaa agcgacagct tatcttgcgg tacaactgaa aaaaacaccg gaagcggctg
     6121 ctgagtttgc tgcaaagatg caggatgcta ccggaacggc gtcagaagac atgatggggc
     6181 tgttcgacac tatccagaag gcgttttatc tgggcgttga cgataccaac atgttgtcct
     6241 tcttcactaa aaccagttct gttctgaaga tggtgaacaa ggacggtctt caggctgcac
     6301 agagccttgc ccccatcagc gtcatgatgg atcagatggg gatgaacggg gagtcggcag
     6361 gtaatgccct gcgaaaagtt atccagtccg gattaagcgt taagaaaatc agggacgtca
     6421 ataaaatcat ggcccgccag aaactcgggg tacagctcga ttttactgac ggcaaaggaa
     6481 gttttggcgg tcttgataac atgttcaggc aactggcaaa gctgcgaaaa ctgaccgacg
     6541 ttaagcgaac aggtgtactt aaggcaatat ttggtgatga tgccgaaacc cttcaggtgg
     6601 tcaatgcact aatcgataaa ggaaaggatg gctacgatca gatccagcag aagatgaata
     6661 aacaggccag cctgaataaa cgtgttcagg cccagcttgg tacgctgtcc aacctgtggg
     6721 aggcaatgac ggggaccgca actaacggcc ttgcggctat tggcggcgca ttttctggtg
     6781 acgccaaaaa tatcacgcaa tggctggggg agttggggga gaaattcacg aagtttgcgg
     6841 atgaaaatcc ccgggttatt cgcggcgtcg tcgggcttgc tgccggtctt gcgattctga
     6901 aactgggatt gatgggcgtg ggcagtgcca tcagtatcgt cagcaggatc atgtcgatga
     6961 cgccgattgg catgattgcg acggcgattg ccctggctgc gggattaatt atcactaact
     7021 gggatgttgt cggaccttat tttaagaaac tctgggaaac cattagtcct tattttgagg
     7081 ctggctggga acttctgaag aaggtttttg cctggtcgcc gctggggatg gtgatcaata
     7141 actggggacc ggttgttaag tggtttcagg atatgtggga taagctgaag ccaattattg
     7201 agtggtttac cgacagttca ggtgacacgg tcgatgccat taactcggcg cagtggggcg
     7261 cgggtgctta tgatgcttat gggacgggaa taccggcgcg gggatacata ccttatccgg
     7321 cggtggatct ggctcagtca aacaacgcct ccgatgccac aggcccgaat cccttcatga
     7381 ttaacaaagc ttctgcgcca aaagttgatg gtgagatcaa ggtctctttt gtgaattcgc
     7441 ctccgggtat gcgggttatg gaaacgcgat ccagcggttt tgatgtcagc catgatgttg
     7501 gctatacgcg ctttggcagg taatgaaaaa ttaatctgtt aatgagtccc actccggtgg
     7561 gattttttat gtacggagtt tatatgacgt ggaaagacag acttcaggac gcgtcatttc
     7621 gcggtgtgcc gtttaaggtt gaagaagaaa atgcgggaac cggtcgtcgt gtggaaacgc
     7681 acgaataccc gaaccgcgac aaaccctata ccgaagatct gggaaaagtc actttccgcc
     7741 cgtccatcac ggcttatgtg gtgggagatg actgctttga ccagcgcgat cgcctgattg
     7801 acgcgctgaa taaacccggt cccggcacgc ttgtccatcc gacatacggt gagctgaaag
     7861 tctgtgttga cggggaggtt cgggtcagca catcgaaggg tgaagggcgt attgtccgct
     7921 ttgacctgaa gtttgtcgaa gcgggagaac tctcttaccc cacatcaggt gcggcgacgg
     7981 cgcagacgct gatgtcatcc tgttctgcac tggatgactg catcagtgac agtttcagtg
     8041 gtttcagtat cgatggcgtg gcagattttg tgcagaacga cgtcgtcggt aatgccagca
     8101 caatgcttgg gtatgtttct gatgcgatga aagtggtgga ttctgccgta tcggatgtcg
     8161 ccaggctgtt gcagggggat atctcggtac ttctgccgcc gccatcgtca ggcaaaaatt
     8221 tcgttgagca ggtgcagaaa atgtggcgta ccgggaaacg cctttatggt aacgccagcg
     8281 acctggtcac catgatcaaa acgctttccg gtgtcagcct cggcagcgat ctgcaaccgc
     8341 gcggcgtctg gaaaacggac agtaaaacca ccgccacggc gacgcagcag cgtaacgtgg
     8401 ttgccagcac ccttcgtacg accgcaatca gcgaagcggc gtatgccgtc acccgattgc
     8461 ctgcgccaac aacttccgcg gtgatgcaga atgccgcagt ggagcaggca acaacacctg
     8521 cgcagagcac tggctggcct tccgtcacgc atccggcact gaacaatgca ccggcggtga
     8581 aaaacacggt tgacctgccg acgtgggaag aactgactga cattcgcgac acactgaata
     8641 cggcaattga taaggagttg tcccgtacaa ccagtgatgc gctgtttctg gcgctgcgcc
     8701 gggtgaaagc agatctgaat gcggatatca acacgcgcct tgaacagtct gcacggatca
     8761 ttcagcgcac accggatgag gttttacccg cgctggtgct ggcggcgacc tggtttgata
     8821 acgcggcgcg tgacgcggac attatccggc gtaatgccat tacgcatccc ggctttgtgc
     8881 cggtgatccc tctgaaggtg ccagtgcaat gaacgataac gtcacgctac gggtaaatgg
     8941 ccgggagtgg aatggctgga catcggtgcg catcggtgcc ggtattgaac ggctggcgcg
     9001 ggatttcagt gtggagatct cccgccagtg gccgggagat gggggtatca ccacgcttca
     9061 gccgcgcatt aaaaacggtt caaaagtgga agtgctgatt ggtgatgagc tggtgatcac
     9121 cggctgggtg gaggcgacgc ccgttcgtta cgatgcccgt tcggtcagca ccggtattgc
     9181 cggacgcagt ctgacggctg acctgattga ctgtgcagcc gaaccgacac agtttaacgg
     9241 acgagagctg gtacagattg cgcaggcgct tgctgcgcct ttcggcattg aggtggtgaa
     9301 caacggtgcg ccgtcgggtg ttattcctga cgtccagccc gatcacggtg aaacggtgat
     9361 tgaggtaatc aacaaaatac tcggtcagca gcaggcactg gcttacgacg acccgcacgg
     9421 caggctggtg attggtggta ttggctcaac gcgggcacat actgcgctgg tactcgggga
     9481 aaacatcctt tcctgcgata cggagaagag tatccgggag cggttttctg tttaccaggt
     9541 ggcggggcag cgtgccggaa acgacgatga tttcggtgag gccaccacca ccgcgctgcg
     9601 ggcccgcaca gaggacgcat ttattgcccg ttaccgtccg atgtatatca ggcagacagg
     9661 gcaggccacg ggggcaggct gtattgcgcg tgctgacttt gaagcccggc aacgggcggc
     9721 gcggacggat gaaaccacct atgtggtgca gggctggcga cagggtaacg gtacgctgtg
     9781 gcagcccaac cagcgggtga ttgtcttcga tccggtctgt ggttttgaca ataccgaact
     9841 gcttgtctcg gaagtcacgt ttactcagga ccagaatggc actctgacgg aaatccgtgt
     9901 cggcccgcct gatgcttatc tgcctgaacc cgaagacccc ggcgcgcgga aaaagaaaaa
     9961 agccagagta caggaggacc cgttctgatg aggacgattg aagccatgca gcgacaactc
    10021 ctcggcctga ttgggcgggc cgtggtgaaa agcatcagtg ccgccacgaa atgtctgacc
    10081 gtggatgtgt ccctgattgc cggtgaaccc aaagccgggg ttgaacatct tgaaccctac
    10141 ggttttaccg caagggcaaa cagcggtgcg gaagcggtgg tgttgtttcc ggatggcgac
    10201 cgttctcatg cggtggttgt tacggtgtca gaccggcgct accgcctgaa agggctgcag
    10261 acgggtgagg tggctgtcta tgacgatcag gggcagtccg tgacgctgac ccgggagggg
    10321 atcgtggtgg acggtgcagg taaaacgatc acgtttcgca atgcacctga agcacgtttt
    10381 gaaatggacc tggaagtaac aggacaggtg aaagacctgt gcgactccgg cggcaccacc
    10441 atgtcagcaa tgcggcttgc ctataacggt catcgtcaca gagagaacgg tcagggcagt
    10501 aacaccgata aacctgataa agctatggag gcatgatgga actgtggctg acggtgaacg
    10561 gtaaacgcac ctgcgccagc gcaccgctgg atccgctgac ccgcgccgtg gtgatttccc
    10621 tgtttacctg gcggcgggcg gagcctgatg acaacgccga cgtcccgatg ggatggtggg
    10681 gggatacctg gcctgcggta cagaatgacc gttacgcctc cagactgtgg ctgcttcagc
    10741 gcagcaaact gaccaatcag ctggtgcaga cggtaagggg gtatatccgc gaatgcctgc
    10801 aatggatgac tgatgacggc gtggtgtccc gtattgatct ggatatccgc cgcaccggga
    10861 ttaatgaact gggtaacagt atcactctct ggcgtcgtga cggaccggta atgatttctt
    10921 ttgatgatct gtggagtgcg ataacgcatg gcggacagtg aatttcagcg cccgacgctg
    10981 gcagaaaata tcagtatgct ccgtaacgat ttattcgcca ggctggacgt cagcgacacg
    11041 ctccggcgca tggatgaaga cgtgcgggca aaggtgtatg cggcggcgct gcatacggtt
    11101 tacgggtaca tcgattatct ggcaatgaac atgctgcctg acctgtgcga tgagttctgg
    11161 ctggcgcgac atgctgcgat gaaacggtgt ccgcgcaagg gggccacggc tgccagcggg
    11221 tatatgcgct gggaaggtgt cagcgatggc ctgaaggtga ccgccggaag tgttattcag
    11281 cgcgatgacc tggttcagta cacggcaact gccgatgcaa ccagctccgg tggtgtcctg
    11341 cgcctgccga tcgcctgttc aactgcaggc gcggtcggta acgctgacga cggtacggca
    11401 ttaatcctgg tcacgccggt gaatggtctg ccgtcttccg gtgtggcaga taccctgaca
    11461 ggtggatttg atactgaaga tctggaaacg tggcgcgccc gcgtcattga gcggtattac
    11521 tggacgccgc agggcggggc tgacggggac tatgtcgtct gggctaaaga agtgcccggc
    11581 attacccgcg catgggcata ccgtcactgg atgggaacgg ggactgtcgg tgtgatgatt
    11641 gccagtagtg acctgattaa tcccattccg gaagaatcaa cggaaacggc ggcaagacaa
    11701 catatcgggc cgctggcccc ggtggcaggc tctgatttgt atgtgttcag gccggtggcg
    11761 catacggtgg attttcatat ccgtgtgacg ccggatacac cggaaatacg ggctgccatt
    11821 accgcggagt tgcgttcgtt cctgctgcgt gatggttatc cgcagggaga actgaaggta
    11881 tcgcgtatca gtgaggcgat ttccggtgcg gacggggaat acagccatca gttgcttgca
    11941 ccggtggaca atatctccat tgcgaaaaac gaactggcgg tactggggac gatttcatgg
    12001 acgtgacaaa cgatgattac atccgcctgt tatcggcact gttgccgccc ggtccggcgt
    12061 ggtcatctag cgatccggcg attgccggtg cggcaccgtc attaacccgt gttcatcagc
    12121 gtgcggatgc cctgatgcgg gagctggatc cgcgcaccac cactgaactg ataaaccgct
    12181 gggagcgtct gtgcggtctg ccggatgaat gtattcccgc aggaatacag acccttcgcc
    12241 agcgtcagca acgactggat gcgaaggtta acctggcggg cggcatcaat gaggattttt
    12301 accttgcaca gcttgctgcc ctgggcagac aagacgccac catcacgcga tacgacaaaa
    12361 gcacgttcac ctgctcatcg gcctgtactg acgcggtgaa tgcgccggaa tggcggtatt
    12421 actggcaggt caacatgcca gccgccacca acagcacctg gatgacatgt ggcgatccct
    12481 gtgattccgc actgcgtatc tggggcgaca ccgtcgttga atgtgtgctt aacaaactct
    12541 gcccttcgca tacctacgta atttttaaat atccggagta atccatgcat cgtatagaca
    12601 cgaaaaccgc gcagaaggat aagttcggcg cgggtaagaa cggttttacc cgtggtaacc
    12661 cccagaccgg cacacctgcc accgatctgg atgatgacta ctttgacatg ttgcaggagg
    12721 agctttgcag cgtggtggag gcatccggtg ccagcctgga gaaggggcgg cacgatcagt
    12781 tgcttaccgc acttcgcgcg ctgctgttaa gccgcaagaa tccgtttggc gatatcaaat
    12841 cggatggcac ggtgaaaacg gctctcgaaa accttggttt gggagaagca gctaaaagga
    12901 atgtaggtac aggggcgaat cagatacctg atatgagcct gttcgcgtca attaataccg
    12961 taacggctgc tgcgcaaaaa tttccgtctg gattaatttt acagtgcggt cagttgaatg
    13021 gtgccccgaa tgtatcttca acatacggga tgaggttccc gatgacgttc tcaagagtca
    13081 ttgctgtcgt agttacattg aacgttactg gcgcggcagg gcagccgact gtatcggcga
    13141 caagtgtcca gaacactgga tttaatatta cggtgtcgcc cggttcagga tacggttcat
    13201 ctgctgatgc gtattacatt gcaatgggat attaacaaaa tgtcatattt ttattctgca
    13261 tcgacaaacg gattttattt gactgaattt cacggcacca atattcctga tgatgcagtg
    13321 gaaatctcgg aatcagagtg gaaaacactg attaatgcac agagtgtaac aaaaatgatt
    13381 acctgtggtg agaacggtca tcctgtcatt gttgaccgtc cttctccaac accagaacaa
    13441 ttagccttaa taaatgatga aaagaaatct gcactgatag cagaggcaac gaatgtaata
    13501 gctccgcttc aggatgcggt tgatttaggt atggcaacag atgatgaaac gaaactgtta
    13561 ctggcatgga aaaaatatcg ggtgctgctt atgcgtgtta atgtagtaaa acccgagtgg
    13621 cctatgcatc caaataaata gacttgttaa tctaggataa ttgttggaat aatactcatt
    13681 tttcagtagg ttcaggtagc ctgggtataa tgctcacttc tacaatgatt tcaacttatt
    13741 ttatttaatt gatattacga aacttaactc atacataaac aatatgactc aatactgtcc
    13801 taaatagctg cgcagaaaag tgctccactt tcgctcagcc cttgctgggc gaagcatcag
    13861 gaaggcgttc tcagctaggt tgtgttatat ctggcttgag ttttgttgcc catacgcaac
    13921 acattaccca tctgtaaacg acaagtgtga cagaattagg tgcagctatt tagactatat
    13981 cgacgcagta acgataatag atatcgaaac tgtactagat attagctact tatggttacc
    14041 gggggaagtc ttttgagcac tggtgttatc aatcggcttc aaataattca ccaataaatt
    14101 atattgcgat agctcatcac cttcctggtt gtttatagcc agtttatcag cgtcaccacc
    14161 tactgtttat gcttttattt ctttgattaa tgagtatatg atattttaat tcatctggtg
    14221 tatttctttc tcatgtgttg gccgactgaa tatctgttca gtgaaggggt aattttatgt
    14281 ttgcacttcg aacattccct tcagttgcca agaaagaaaa ccgtaacatt tgcgcattaa
    14341 ttttaacagt ataatctgag cataaatatg cgctatatat ctataaaaag gcgcaattaa
    14401 ttgcgccttt ttatcatttt atcaaaaatg gcccaccgac tgtcctcgat agccaatggc
    14461 caattttatc tgatacatat acataaatac aggtatatat gattgcgcag ataggatttg
    14521 gaatggtcac gctgaagcct atgattgcta aagccatagg gataaataat ttggtgatat
    14581 actccattcc acatatgtta agagtatttc ttccagctct tacaataaag tcattcccac
    14641 aaattatttt tgctataaaa atattaaata ttatcaaacc taccgtggtg ataatagtat
    14701 taatagcttg aatataacta cttgatatta tgtctgcatt gaatattgag ataatcttaa
    14761 aaagcaaagg tttctggtta aggagttgat acgcactcat tatcgcggta attgagaaga
    14821 caatgaaacc aaat
//
LOCUS       DAJTYF010000097        14677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTYF010000097 DAJTYF010000000
VERSION     DAJTYF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.097"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            158..1198
                     /locus_tag="OO590_003039"
     CDS             158..1198
                     /locus_tag="OO590_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6638331.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1231..2424)
                     /gene="lplT"
                     /locus_tag="OO590_003040"
     CDS             complement(1231..2424)
                     /gene="lplT"
                     /locus_tag="OO590_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6638332.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2417..4576)
                     /gene="aas"
                     /locus_tag="OO590_003041"
     CDS             complement(2417..4576)
                     /gene="aas"
                     /locus_tag="OO590_003041"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6638333.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            4965..5996
                     /gene="galR"
                     /locus_tag="OO590_003042"
     CDS             4965..5996
                     /gene="galR"
                     /locus_tag="OO590_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6638334.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6003..7265)
                     /gene="lysA"
                     /locus_tag="OO590_003043"
     CDS             complement(6003..7265)
                     /gene="lysA"
                     /locus_tag="OO590_003043"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6638335.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7387..8322
                     /gene="lysR"
                     /locus_tag="OO590_003044"
     CDS             7387..8322
                     /gene="lysR"
                     /locus_tag="OO590_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6638336.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8309..9001)
                     /gene="ygeA"
                     /locus_tag="OO590_003045"
     CDS             complement(8309..9001)
                     /gene="ygeA"
                     /locus_tag="OO590_003045"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6638337.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAS
                     IHAEDAVAFMLS"
     gene            complement(9130..10518)
                     /gene="araE"
                     /locus_tag="OO590_003046"
                     /pseudo
     CDS             complement(9130..10518)
                     /gene="araE"
                     /locus_tag="OO590_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(10833..11594)
                     /gene="kduD"
                     /locus_tag="OO590_003047"
     CDS             complement(10833..11594)
                     /gene="kduD"
                     /locus_tag="OO590_003047"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6638338.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11624..12460)
                     /gene="kduI"
                     /locus_tag="OO590_003048"
     CDS             complement(11624..12460)
                     /gene="kduI"
                     /locus_tag="OO590_003048"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6638339.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(12780..13928)
                     /locus_tag="OO590_003049"
     CDS             complement(12780..13928)
                     /locus_tag="OO590_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6638340.1"
                     /translation="MKDVVVVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            14183..14677
                     /locus_tag="OO590_003050"
                     /pseudo
     CDS             14183..14677
                     /locus_tag="OO590_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
BASE COUNT         3803 a         3977 c         3472 g         3425 t
ORIGIN      
        1 acgcgatgca aattaaccgt gatgatgttt cgcaaattat tgaacgttaa caaataaggt
       61 cagcatccgg ctggccttaa gatttttctc tttccctttt cccttccctc tgccattttt
      121 atattcctta tgtcgtgatt ataaaaagga aacggctatg caatatcacc gtatacccca
      181 cagttcgctg gaagtcagca cgctggggct tggcacgatg acgtttggtg aacagaacag
      241 cgaagccgac gcccacgcac aactcgacta tgccgtcgct cagggcatta accttatcga
      301 cgttgccgaa atgtacccag tacctccgcg ccccgaaacg caagggttaa ccgaaaccta
      361 cgtcggcaac tggctggcga aacatggcag ccgcgaaaag ttaattatcg cctccaaagt
      421 gagcggaccg tcgcgcaata atgacaaggg catccgcccg gatcaggcgc tggatcggaa
      481 gaatatccgc gaagcgctgc atgacagcct caagcgccta cagactgatt acctcgatct
      541 ttatcaggtg cactggccgc agcgcccgac caactgcttc ggcaaactcg gttatagctg
      601 gacggattct gcgcctgcgg tttcgctgct ggatacgctg gacgcactgg cagagtacca
      661 acgcgcggga aaaattcgtt atatcggcgt gtcgaacgaa actgcatttg gcgtaatgcg
      721 ctacctgcat ctggcggaca aacacgatct gccgcgtatt gtcaccattc agaaccctta
      781 cagtctgtta aaccgcagtt ttgaagtagg tctggcagaa gtcagccagt atgaaggggt
      841 cgaactgctg gcctattcgt gcctgggttt cggcacgctg accgggaaat atctcaatgg
      901 tgcaaaaccc gctggcgcac gtaatacgct ctttagtcgg ttcacccgct atagcggtga
      961 gcaaacgcaa aaagccgtcg cggcgtatgt tgatatcgcc agacgtcatg gccttgatcc
     1021 tgctcagatg gcgctcgcgt ttgttcgccg tcaaccgttt gttgccagca ctctgctggg
     1081 cgcaaccacg atggatcagc tgaaaactaa catcgaaagt ttgcatctgg agttaagcga
     1141 agacgtatta gctgaaattg aagcggtaca tcaggtttat acttatcctg caccataaag
     1201 attaacgccg gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga tccacagcgc
     1261 cgttattgcc agcgcaaata gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc
     1321 taccattacc gccagcgagt aaatgcccag catcaacaac atggcgctgt tttcgccaag
     1381 gttttgtact gcaatcgcat tccccgcccc gacgcttttt ttaccccgct cctgtagcaa
     1441 cgcattgagc ggaacgacaa aaaagccccc cagcacgcca atcagcatca acaaggcata
     1501 ggctggcagc agctcgtgtt gcagggagaa aatcagtacc accacgccaa tcaaaatccc
     1561 ggctggcata cagcgtgaca cggtttccag cgtaaccagc ttcgctgccg cacctgcgcc
     1621 aaccacgata ccaatcgcta ccatcgcgtt gagataggtc ggcgtagcgt tatcggtaat
     1681 gcccagcgcc accggtaccc acagcaccaa caggaaacgc agcgtgacac ccgctcccca
     1741 gaataaactg gtgcccacca gcgaaaaacg cgtttcacca ttgcaccata gcgaggtgca
     1801 ggcattcagg aaactgcggg tcatgttgat gagattccag gactgccccg gacgcgccgc
     1861 cgccagtttg ggaatgtaga tattggcaac gaccgcacca ccgtaggcca gtgcgcatgc
     1921 ggccagggcg accaggacat gccagtcagc cagcacacca ccggctacgg aaccgagtaa
     1981 aatcgccgct atggcagaag cttccattaa accgttagct tttactaact tactacccgt
     2041 ggttaattcg ccgagaatac cgtatttcgc cggtgaatag gctgcagcac caacacccac
     2101 cagcgtatag ccgagaaacg gattgatacc aaagcagata ctggctgcgc ccagcagctt
     2161 caggccgttg gcaaacatca tcacccggcc tttggcgaag ctatccgcca cctgcccgac
     2221 aaacggcgcc aaaagaatgt aagcacctac aaacaccatt tgcaggatgg gctggctcca
     2281 ctccggatag aactgcgctt tcagtaacgc cagagtggca aacagtagcg cgttatcgcc
     2341 aaacgcagag agaaactgcg ccacgataac cgctttcatc cccttcgacc acaacgaagt
     2401 gttagtgtgc actgactcac tcatcgtgtt gttccgcttc gtctacccag cttttcaacg
     2461 tgacaaagtc aggtttgccg ctgccaagta atggcatctg tttcagatag cgaatatcgc
     2521 gcggtacagc aagctccggc acgccgtgct cgcgggcata ctgttgcaac ttatcgcgcg
     2581 tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc tttgctggca tcgctcttaa
     2641 tcgcagtggc atggacttta tctggcgaaa caccaagtgc cagttgttcc accatttcca
     2701 gcgacaccat ttcgcctgca attttggcaa agcgttttgc gcggccctga atctgcacaa
     2761 agccctgctc gtcaaaacgc acaatatcgc cagtgtcata ccagccgcgc tccatttcgc
     2821 cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg cttctccacc cgcagatagc
     2881 cgttcattat gttcggccct ttcagttgca ggcgtccgcc ctcttcgata ccagggaccg
     2941 acaacaggcg tgcatccatt cctggtagaa tacgccctac cgtaccgggt ttcgccgcca
     3001 tcggtacgtt gatagaaacg acaggcgcgc attcggtcac accgtagcct tcaaggatgc
     3061 gcaggccaaa tttatcctgc caaagctgtt tggtactttc ttgtagtttt tctgcgcctg
     3121 ccaccacata gcgtagacga tagaagtcat acgggttggc gaaacgcgcg tagtgaccga
     3181 ggaaagtcga ggtaccgaac aacacggtgc aactgcggtc atacaccaag tccggcacaa
     3241 tgcggtaatg cagcgggctt ggataaagga acacttctgc acctgtaagc agtggcgtaa
     3301 acaggcctac cgtcagccca aaggagtgaa acagcggtaa tgccgacata aagcgatcgt
     3361 tggtggtgaa gtcggcaatc gttttaatct gctcgacgtt cgccagaatg cttttatggc
     3421 tatggacgac gccttttgga tggccttcag aaccggaggt aaagaggatc agcgcctctt
     3481 cttccggctg ctgttttacc tgcgccaggc gcggcatcag caaatgagcg aagatccata
     3541 ctttgtcggc agtggtgaca tctgctttta aatcttccag atagacccag cgcacctgag
     3601 taagttgctc cggcagatgc cagagtttgc ctttatcgag aaactggcgg gaagtgaaga
     3661 tggttttgat ttcagccgcc gtaatagcac tggtcagccc ttttaccccg gcagtgtagt
     3721 tcatcattgc gggaatgcgg cgacgcgcga tggccccaaa aatcactgcc gcactgatgc
     3781 ctgcattggg cagcattaag ccgatgcgtt cgccttcaac actgtatttt tcaaggatgc
     3841 gtccaacaaa cagcgttttc gtaagcaatt tgcgatagga gtctggggta aagttgacgt
     3901 cttcgacaca tttcttcccg gctccgaagc ggtacattgc actcagtaaa gattcgtaca
     3961 gagtttcacg cgggcgcacc gccatacgcg cttccatcat tatttgatgc agcatttcgc
     4021 cagcgatttt gcgacggtca cgggcacgcg gcgcatccgg catcgccacc tgcgttggag
     4081 gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt aaccagacct ttcaggcggc
     4141 tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat aaccgttgca ccagacttcg
     4201 ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga gcctgtagtg gtgatgcgtc
     4261 cttcagggaa aatcaccact ggtcggccct gttccaccag acgtaccaga tgtttaatag
     4321 ccataggttg cgtcgggtcg agaggaacaa agtcgataaa tgatttcagc caacgcatat
     4381 accactgttg gcttattgag gtgtaaacgg caaacactgg acgcacaggt aaaaacagtc
     4441 caagcaaaat gccatcaata aaagagacgt gattaggcgt aattagaacg cgctcgccct
     4501 tcagtgcctg ggtgtcaccc gtaacgcgaa cgcgatacaa aacacggcac aaatttcgaa
     4561 aaaagctaaa aagcattcca actccctttg ctctgattca gtaaaagcga atggagggag
     4621 attacacgag ataaagaacg cgagcgacag taaattaggt gcgaaaaaaa acctgcgcat
     4681 ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta ccgaataatc tcaccaatca
     4741 atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagcg gtcaatcgca
     4801 acagcgtttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct tacttatttg
     4861 cgtttggctc acattcccac gatgaaaaca cgccacccct tgaaccaacg ggcgttttcc
     4921 gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg accataaagg
     4981 atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac
     5041 ccaaagccag cgaagcttcc cggctggctg tgcatagtgc aatggagtct cttagctatc
     5101 acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg
     5161 ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa caggtggctt
     5221 atcacaccgg taatttctta ttgattggca acggttacca caacgaacaa aaagagcgtc
     5281 aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga
     5341 tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg ctgatcaacc
     5401 gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac ggtgcctggc
     5461 tggcaacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg tgctctaacc
     5521 actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt gctgaaagtg
     5581 gtattccggc caatgatcgg ctggtgacat ttggcgaacc agacgaaaac ggcggcgaac
     5641 aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc tgttataacg
     5701 attcaatggc ggcgggcgcg atgggcgttc tcaatgataa tggtattgat gtaccgggtg
     5761 agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga
     5821 ccaccgtgcg ttacccaatc gtaacgatgg cgacccaggc tgccgaactg gctttggcac
     5881 tggcggataa tcgccctctc ccggaaatta ctaatgtatt tagtccgacg ctggtacgtc
     5941 gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc gactaaccgc
     6001 agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg aatcaaccgc
     6061 gcctgaccat tatcaaacag aacttctggt aacagcggac ggctattgta gttggatgac
     6121 attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacctgc cttcacttcc
     6181 ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc gcccgattca
     6241 cataacggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga acgaccatca
     6301 gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat cagatcgttg
     6361 aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct ccgcacctga
     6421 gtaattaata cgccagactg cgctaccagg aagcgacccg gttcaatttc cagtttcaca
     6481 gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc ataataatgt
     6541 tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc gcccgcagaa
     6601 atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc acacacctgc
     6661 tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac tagctgcaga
     6721 tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg
     6781 ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc cggattaacg
     6841 cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat atcaacagaa
     6901 cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg atcgataaca
     6961 tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc cgccaacgca
     7021 cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg cattaagcgc
     7081 aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacttcaaa ctgtttcagc
     7141 gctgctatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca gccaaattca
     7201 gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt
     7261 ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata aaaagaataa
     7321 aaaatatcga tttatgtcga atctatgcaa aaatgatatg gattaccgga ttgcgagaga
     7381 gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt aatgaccgcc
     7441 ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt cagccgcgaa
     7501 ctggcgcgct ttgagaaggt gatcgggctg aaattgtttg agcgcgtacg tggacgatta
     7561 catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg gtacggactg
     7621 gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga actgtctatt
     7681 gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc ctttctggca
     7741 cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact tgaagagtgg
     7801 ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc tgcgggaaca
     7861 gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg
     7921 gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaattacat cagcctttcc
     7981 cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca ggttaaacga
     8041 cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg ggcgggggta
     8101 ggtgtttcgg tggttaaccc gctcaccgca ctagattatg cggcaagcgg tttagtggtg
     8161 cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct gcaccgcccg
     8221 tcatcagcgc tggttcaggc gtttagtggg catttacaag cggggttacc gaaactggtc
     8281 acttctcttg acgctatttt gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg
     8341 catggatcga cgcggtatca aacacaggca aaacactgcg ctcttctggc accagtaaac
     8401 caatttctgt gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg cgagcaatca
     8461 cttgcgcata ataagcgcgt gacgcttcgg taaatttccc cagacacagt tcttcaaaaa
     8521 taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg atggaaaatt
     8581 gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta cccagcagcg
     8641 ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca atgtgtaaaa
     8701 aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta ttggtacata
     8761 acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc agaatgtccc
     8821 cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa tcgacgctat
     8881 gtagcagcat ttgcgcagag tgaagcccac caagccgctg tttaatgcct tcatttatca
     8941 gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca attgttttca
     9001 ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag acaaacgcct
     9061 caacggccag gtgcccaatc tctattaacg aaaaaagggc cggatgtaca gcacatccgg
     9121 cccgtgaaat cagacgccga tatttctcaa cttctcgcct gccatcagtt tgcgttcgat
     9181 atgttccagc gtgacatttt tggtttccgg aatgagccag aaagtaatgc ccacaaacgc
     9241 aatgttcaac gcagtgtaga gccagaacgt accggcagcg ccaatgctat caagcagtgt
     9301 caggaaggcc gcgccgataa tcatattcga cacccagttt gtcgtcgtcg aacaggtaat
     9361 accgaaatcg cggcatttca gcggctgaat ttcagagcac aggatccaca ccactggcgc
     9421 ggcgctcatc gcataaccgg caatacacat catcgtcatg ccaacagaga gccaggacaa
     9481 gccactggaa gccgtaccgt tatcaaactg catcaggcaa tagcccagca ccagagtgcc
     9541 taacgccatc acgctgaaac caattttcag agccggttta cgacctgctt tatctaccgt
     9601 aaagaccgca atgaaggtcg cgaacataaa ggtcagtccc acgaccagag tcgcaatcat
     9661 ctgttgttct gtggtcgtaa agcccgccat tttgaagata cgcggcgcgt agtacatgat
     9721 gatgttcata ccggtaaact gctgcatcgc ctgcaacaac ataccgagaa acacagcacg
     9781 acggacgtta cggttgatct taaacagtgc ccaaccgccc tgttttaact tcaggctttc
     9841 acgaatttcg ttgagttctt ctcgcgcttt ttccgacgta tcgcgcagca tacgcaacac
     9901 ttcttccgcc tcaatatgac gccccttttc cgccagccag cgcgggctat ttggcaggaa
     9961 gaccaccaga ataatcagca gaactgctgg taaagcaaga accccaacat tgcgcgccag
    10021 ttaccgctat aactgaacgc tgtatcggat aaaaacgcca gcacgatgcc gagtgtgacc
    10081 atcaactggt acatactgat catcttaccg cgaacgtact tgccatttca gaaagataca
    10141 gaggagcggt gtaagacgcg atcccgacag caatgcccag caccacacga gcggcgatta
    10201 acatctctac gctggtcgca aaagcggacc ctatagaacc gagtacaaac aggatggccc
    10261 caggcggaac gacagccaac cattaaacag cgcaccaatt gctgcgccga gcatcatgct
    10321 actaaccacc cattcctgca aacgactggt cagcacaaag tgatcggtaa tgaacggcaa
    10381 cgctccggcg attacgccga tatcaagacc aaataacaat cctgcgaccg cagcagctac
    10441 cgaaacaaac atattcatac gccgcgtatc acgcaaagaa cgtggcgtta aagcagattc
    10501 cgtattgata gtaaccattt tttcctgcca gcagagagta agacattgtg aaaaaatacg
    10561 tgaacaattc acacaggtgt caggtcggaa acagcataaa tatggattaa attgctgcga
    10621 catgccgtta tgtgatggat attccaattt taaaattaag ttgaattatt gagattatta
    10681 ttaaccacct aattttaaag tagataaaat tcataaagtt cattaattga taattgatat
    10741 ggattatttc ataaccatga tatggattat gatgatctag agatataaaa aaccctgcca
    10801 tgcggcaggg tcataaaagt aagaggaatg aattaacgcg ccagccaacc gccatccaca
    10861 gcaatggtat aaccatttac ataatctgaa gcgctggagg caagaaacac taccggcccc
    10921 atcaggtcac tcggcagtcc ccaacggcca gctggaatgc gatcgagaat ttccgcgcta
    10981 cgttgttcat ctgcccgcag ttgttgggta ttgttggtcg ccatgtaacc cggagctatc
    11041 gcattaacat taatgttgtg tttagcccat tcgtttgcca tcaatcgcgt cacacccatc
    11101 actccgcttt ttgatgcggt ataagaaggc acacggatcc cgccctggaa ggagagcatt
    11161 gacgcgatat tgataatctt gccgccattg ccttgcgcga taaagtgttt cgccgctgcc
    11221 tgagacatga agaatacgct cttgatattc aggttcatga catcgtccca gtctttttcg
    11281 ctgaactcga gagcgtcttc gcggcgaatc aatccggcgt tattcaccag gatatcaata
    11341 tgaccaaatt cagctaccgc gcgatccagc agtccaggaa tgccatcaat ctttcgcaga
    11401 tcggcggtca ggcttaaaaa acgacgcccc agcgccgtga cctgctcgat ggtttcagtc
    11461 ggttcaacga tgttaatgcc aacaatgtca cagcccgctt gcgccagccc caacgccatc
    11521 ccctggccca gcccagtatc acaaccagtg acgaccgcaa ctttaccttg gagagaaaat
    11581 gcacttaaaa tcataacaac accttattcg tttatgccca caactagcgc aaatctttaa
    11641 cggccacatg gtccatatca ttaaagacct ggttttcacc gaccatgccc cagataaagg
    11701 tataagcttt ggttccgacg ccggaatgga tcgaccagct cggggagatc accgcctgct
    11761 cgttatgcat cacaatgtga cgcgtttctt gcggctgccc catcatgtgg aaaacgcagg
    11821 cgtcatcatc catattgaaa tagaaataaa cttccatccg gcgctcgtgg gtgtgacacg
    11881 gcatggtgtt ccacaagtta cctggagcca gctccgtcag ccccatactc aattgacagg
    11941 tttccagtac atccgggaca aaatatttgt taatcgtgcg acggttactg gtgaggttat
    12001 cgcctaacgt gactggagat acttcgtccg gtgtgacttt tttggtggga tacgtcgtat
    12061 gcgcgggtgc gcaattgtaa taaaacttcg ccggagtggc ggtatcaata ctggcaaaga
    12121 caacttcttt tgcaccttta ccaacataca gggcgtcgcg gtgaccgatt tcatagcatt
    12181 gaccatcgac agtaatcgta ccggcaccgc caatattgat aacacctaac tcgcgacgtt
    12241 caaggaaata gcttacgccg agttgtttac caacttcccc gccaacggaa accgttttag
    12301 ttatcggcat aatgccgcca acaataattc ggtcaatgtg gctgtaaacc atggtgtact
    12361 catcggcgac aaataccttt tcaaccaaaa attcattacg cagcccttgg gtatccagcg
    12421 tttttgcgtg cgcaccgtgg atgctctgtc ttacgtccac atcaacctcc gataatcagt
    12481 gaagcgtgaa aatagaaaac gatagtgaaa cactgtttcg ttttctctat aaagggatga
    12541 tatgcccatg ggctagggtt ttcaattaaa aataaaacaa tgttttattt ttaatttaag
    12601 gattaaaaaa agtgcagtgt cgatcacgaa tatgtcatga gaatggaaaa atgagggagc
    12661 ccagaaaatg tatgtcgact tgccagccat cattattcaa cagattaatg acggcagata
    12721 tttatcggta aggaggaaat gcagagctac tcgtcacgtt caatggtcaa tgccacgccc
    12781 tagcccccgc cgatacaaag cgttgccagc cctttgcggg cattacgttt caccatttca
    12841 tgaaccagag aaaccaggat tcggcaaccg gaagcgccta tcgggtgacc gagtgcgatc
    12901 gcgccaccat tgacattgac ccgacgctca tcccactcaa gcatcttgcc aaccgaaagc
    12961 gcctgtgcag caaacgcttc attagcctcg ataagatcga catcagccaa ctgccagcct
    13021 acacgctcca ggcaacggcg ggtcgcatac accggcgcaa ttcccatcaa tgccggatct
    13081 acaccaacgc tggcaaatgc gcggatgcgg gccagcacgg gtaaattcaa cgctcgtgct
    13141 ttggcttcgc tcatcatcat taccgcagct gcgccatcgt ttatggatga tgcattcccc
    13201 gcggtcacag aaccgagact atcaaatgaa ggatttaaac gggctaaacc ttctgcactg
    13261 gcgtcagtgc gtggctgttc atcggtatca acaaccaacg tctgaccgtt actttggatc
    13321 attaccggga cgatctcatc tttaaatcgt ccggcgtcaa tcgccgctcg cgctttttgt
    13381 tgcgagctaa gtgcgtaagc atcctgcaac tgacggctga tgccatattc gcgagccaga
    13441 ttttcggcgg tgacaccaat atgataatca ttgaaggcat cccacaaccc atcatgcacc
    13501 agactgtcaa ccaactggct attgccaagc tgtgcaccgg tgcggctatc agtcagaaca
    13561 tgtggtgcgc ggctcatgtt ttcctggcca ccggcgatga caatatcagc ctcgccacac
    13621 tgtatcgcct gagtagccag atgcagtgct ttaagaccgg aaccgcaaac gtcattaatc
    13681 gtgattgcag aaacgctgtt aggaagacca cctttaatag ccgattgcct tgccggattc
    13741 tgccctgccc ctgcagtcaa cacctgacca agaattactt catccaccgc atatgcagga
    13801 acgccggtac gttctattaa cgctttcacg accagactac caagttccac ggcggaatga
    13861 cccgctaacg caccacgaaa gcagccgata ggtgtccgta acgccccgac aaccacaacg
    13921 tctttcatca caacctcgca ccaattaaca gtgcaatagt aaatgattgt taattacagt
    13981 tatcttaatt gtttaaaaaa agtgattttt atcacaaagg aaatatgcct gagcagtagt
    14041 cagagtcata actggcacgt aaggtttgca accactaacc caccaataga tgggtagata
    14101 gggcgttaat ctcccatact taacttggtt tatggtaaat tgccctccat tttgtttaat
    14161 ttgtagatga tacgttcaga taatgtctaa tatttggtct aaagaagaaa ctctgtggag
    14221 tttcgcgctc tacggcacag ccgttggtgc aggcacgcta ttccttccta ttcagttagg
    14281 ttcggcaggg gctgtggtcc tgtttattac tgctctggtc gcctggcctt taacatatta
    14341 gccacataaa gccttatgcc agttcatcct ctcatcaaaa acatcagcag gtgaagggat
    14401 aacgggcgcg gtaacacact actatggcaa gaagattggt aatctgatta ccacgctgta
    14461 cttcatcgcc ttttttgtcg tcgtgttgat atatgcagtg gcaattacca actcacttac
    14521 ggaacagctg gcaaagcata tggttattga tcttcgcatc cgtatgttgg tgagtctggg
    14581 tgttgtatta attctgaatc tcatttttct gatgggacgt catgccacta ttcgggtaat
    14641 gggatttttg gtattcccat tgattgccta tttctta
//
LOCUS       DAJTYF010000098        14527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTYF010000098 DAJTYF010000000
VERSION     DAJTYF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.098"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(337..582)
                     /locus_tag="OO590_003051"
     CDS             complement(337..582)
                     /locus_tag="OO590_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638341.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="OO590_003052"
     tRNA            complement(1227..1302)
                     /locus_tag="OO590_003052"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="OO590_003053"
     CDS             complement(1408..2115)
                     /locus_tag="OO590_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6638342.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="OO590_003054"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="OO590_003054"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6638343.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LVIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVVMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OO590_003055"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OO590_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6638344.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OO590_003056"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OO590_003056"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6638345.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="OO590_003057"
     CDS             complement(7299..7574)
                     /locus_tag="OO590_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6638346.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OO590_003058"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OO590_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6638347.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQMLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPKFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="OO590_003059"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="OO590_003059"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6638348.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="OO590_003060"
     CDS             9534..9860
                     /locus_tag="OO590_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6638349.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="OO590_003061"
     CDS             10044..10763
                     /locus_tag="OO590_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6638350.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="OO590_003062"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="OO590_003062"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6638351.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13109
                     /locus_tag="OO590_003063"
     CDS             12102..13109
                     /locus_tag="OO590_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6638352.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13165..14466)
                     /locus_tag="OO590_003064"
                     /pseudo
     CDS             complement(13165..14466)
                     /locus_tag="OO590_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3807 a         3476 c         3734 g         3510 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgccggttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aagtagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctggtgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctctg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggtga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac gtcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agtagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgattcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaacttcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcatctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agtacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tgcccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacggcg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaattactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat acaaagctaa gggataatgc gtagcgttca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgacta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    13021 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctggaaaaaa gataccgaac
    13081 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
    13141 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
    13201 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatggcag
    13261 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
    13321 actacccacc ggcagtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
    13381 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
    13441 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
    13501 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
    13561 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
    13621 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
    13681 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
    13741 ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac accagaagca aacttggtaa
    13801 ggcatccaga aacgctttac aggcctgagc aaaggttgct ctttctgaaa gggggtagcc
    13861 acggcgttta gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg
    13921 caaatatccc gccataaaca gcgatgctac agaaacgccg ccagctgtca gggaaaacac
    13981 aattaacacg tttgagggcg gtatcagtaa tccagtaata caagatgaga cgtttacagc
    14041 ggtagaaaat gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac
    14101 ggcagcagct gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac
    14161 gttcacatga ccaagcgaac caggaacgcg gccaaccatt acctaggcga ggttaatcaa
    14221 acgtatcgca ataccgccgc tgttcatcag tgttccggca aagatgaaaa aggggattgc
    14281 caacagggca aagttatcca gtccattagc cagtcgttgc gaaacagtga ttgccgcgat
    14341 atcccaggga aacatcaaaa gcattgaagc tacagtggca ataccgattg agaacgaaat
    14401 gggcacacca ataaagacca gcacaaaaaa acagccaaac agtgtaatcg cgatataact
    14461 atccattaac tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa
    14521 cacaagg
//
LOCUS       DAJTYF010000099        14446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTYF010000099 DAJTYF010000000
VERSION     DAJTYF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.099"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(675..2135)
                     /gene="phoQ"
                     /locus_tag="OO590_003065"
     CDS             complement(675..2135)
                     /gene="phoQ"
                     /locus_tag="OO590_003065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6638353.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2135..2806)
                     /gene="phoP"
                     /locus_tag="OO590_003066"
     CDS             complement(2135..2806)
                     /gene="phoP"
                     /locus_tag="OO590_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6638354.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(2975..4345)
                     /gene="purB"
                     /locus_tag="OO590_003067"
     CDS             complement(2975..4345)
                     /gene="purB"
                     /locus_tag="OO590_003067"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6638355.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4349..4990)
                     /gene="hflD"
                     /locus_tag="OO590_003068"
     CDS             complement(4349..4990)
                     /gene="hflD"
                     /locus_tag="OO590_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6638356.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5026..6132)
                     /gene="mnmA"
                     /locus_tag="OO590_003069"
     CDS             complement(5026..6132)
                     /gene="mnmA"
                     /locus_tag="OO590_003069"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6638357.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGHEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6186..6647)
                     /gene="nudJ"
                     /locus_tag="OO590_003070"
     CDS             complement(6186..6647)
                     /gene="nudJ"
                     /locus_tag="OO590_003070"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6638358.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(6657..7310)
                     /gene="rluE"
                     /locus_tag="OO590_003071"
     CDS             complement(6657..7310)
                     /gene="rluE"
                     /locus_tag="OO590_003071"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6638359.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7482..8732
                     /gene="icd"
                     /locus_tag="OO590_003072"
     CDS             7482..8732
                     /gene="icd"
                     /locus_tag="OO590_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6638360.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(8835..9158)
                     /gene="iraM"
                     /locus_tag="OO590_003073"
     CDS             complement(8835..9158)
                     /gene="iraM"
                     /locus_tag="OO590_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6638361.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(9411..9455)
                     /locus_tag="OO590_003074"
     CDS             complement(9411..9455)
                     /locus_tag="OO590_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638362.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            complement(9691..9801)
                     /locus_tag="OO590_003075"
     CDS             complement(9691..9801)
                     /locus_tag="OO590_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638363.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(9854..10258)
                     /locus_tag="OO590_003076"
     CDS             complement(9854..10258)
                     /locus_tag="OO590_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCR6638364.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10479..11210)
                     /gene="bluR"
                     /locus_tag="OO590_003077"
     CDS             complement(10479..11210)
                     /gene="bluR"
                     /locus_tag="OO590_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCR6638365.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11415..12626)
                     /gene="bluF"
                     /locus_tag="OO590_003078"
     CDS             complement(11415..12626)
                     /gene="bluF"
                     /locus_tag="OO590_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCR6638366.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            12940..13176
                     /locus_tag="OO590_003079"
     CDS             12940..13176
                     /locus_tag="OO590_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCR6638367.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13219..13491
                     /locus_tag="OO590_003080"
     CDS             13219..13491
                     /locus_tag="OO590_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCR6638368.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            13520..13786
                     /gene="ariR"
                     /locus_tag="OO590_003081"
     CDS             13520..13786
                     /gene="ariR"
                     /locus_tag="OO590_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCR6638369.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            13899..14147
                     /locus_tag="OO590_003082"
     CDS             13899..14147
                     /locus_tag="OO590_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638370.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         3867 a         3293 c         3339 g         3947 t
ORIGIN      
        1 cagcttaatc gctaacccgc tctgatattt tcggaaacct caatttgagg aagtcggcga
       61 ggtaactgtt tgatattccc gatagcgcaa aaatgcgcgg tcgggcagaa tgaccaccac
      121 ctcaatcgtg acggagagtg acaaccagtg acacaccgtg acaaaaagac ggtttaaccg
      181 ttcattaaaa atcgacgtga ttttgtgctt tccggatgac gtggccaacg tcatttttta
      241 gcgaaaaatt ctgattaagg tcactggctg aacggtcata atgaccacgc agaccatcga
      301 ggcaaccata gcgatcatcg ttacggttgg agcttttccg ccattggtgg aaaaggtgca
      361 catctggatc atcattacgg tgatcaaggg tatgtaaaac ccaccagcct gattaaaggt
      421 taaccggaaa aaaaatccgg tagccccgtt atggggatcc ccatatcgac attaacgccc
      481 ctcatgaatt ggtgcgcttc cccctggaaa gattacccgc ctgtatattt cttgtgtcta
      541 tttgttccac gttgtttcat acagtgcacc gaacggtgta gttaatggtg tagtcaattt
      601 gcgatttttg gcactttttc aatgaaaatt gtttaatcga atcaatgact tgatgtagtg
      661 gtaaaaggac atatttattc atctttcggc gcagaatgct ggcgaccaaa aatcacctcc
      721 atccgcgcac cgcccagcat gctctctccg gcgacgattt taccctcata ttgctcggtg
      781 atttcgcggg ctaccgccag ccctacacct tgcccagggc gtaaagtatc aacccgttga
      841 ccacggtcga aaatgacctc tcgcttgctt aatggaatac cggggccatc atcctcgacc
      901 acaatataga gatgctcgtc ggtttgcctt gcagaaattt cgacaaactc gaggcaatat
      961 ttacaggcat tatccagcac gttgcccatc acctcgacaa aatcgttctg ctcaccgaca
     1021 aagctgatct ctggcgaaat atcgagagag atattgaccc ctttgcgttg atacactttg
     1081 ttcagcgctg aggtgagatt gtccagcagt ggggcgaccg gatgcagctc gcggctgagc
     1141 aatgtcccgc cgcgcatact ggcacgatgc aggtagtagc caatttgctg tgaaatgcgg
     1201 ctgatttgct ccagcattac cggctcagca tcactgacgc tcatcttttc actacgcaga
     1261 gaacgcagcg tactttgcag caccgccagt ggcgttttca gactatgggt caggtcggtg
     1321 agcgtcgtac ggtatttgtc gtaacgttcg cgttcacttt ttaacaatcg gttcaggttt
     1381 cgtaccagac tggtcagttc tcgcgttgtg gctggattga gcaattcgcg gttatgttct
     1441 tccagttcgc ggacttcttt tgccagggct tcgatggggc gtaaactcca ccaggcggcg
     1501 acccacagca gcgggatcac taacagcaga ttggctgaga gtacatagat aaaccagctc
     1561 cagaccatat aggaactttt tagctccacc ggaatggtat ccaccaccac aatggttaat
     1621 tttggcatcc gcgatgttgc cgggtagacg tttaccgcca ccgagtgggt catctccgcg
     1681 tcgtcatcat cttcccgcac ttcctgcaac tgttgctgta tcgaatgatc tccactcagc
     1741 aagaggctgg tatcgttaac atccgcttca atttcatgaa aaccattcga tttcagccag
     1801 tcaggctgga tcattttcat cagccagggc acgtcacgtt gcgcccataa aagctgcccg
     1861 ttctcatcat aaattagcgt catggtgggg ctttgcttgt cgatattttc gggtaactcg
     1921 acatgcaact tattgttttc ccactgcgca agggtataga acagattgct ctcgccacgt
     1981 aacagccgaa acgtagtttt atcgaaactg acgctataac cgatcagcgc gaccattccg
     2041 taggcaagcg aaagcaccag tactaccgct gccgttgcca acagaaaacg tacccgcagc
     2101 gagagcggga aaaaaagacg cagtaatttt ttcatcagcg caattcgaac aggtagccct
     2161 ggccgcgaac ggtggtaatg acttcttgcg gatattgtgc ctgaattttt ttgcgcagac
     2221 gtcccatcag tacatcaatg gtatggcttt cccgcagctc cgcatccgga tagagttgta
     2281 gcattaacga atctttgctg accactttgc cattattgcg tatcaacgtt tccataatgg
     2341 tgtattcgaa cgcggtcagt ttgatcactt cgtcattaat agataattca cggcgagaga
     2401 gatcaacctg aaacgggggg agcgaaatga cctgtgaagc cagaccgcta ttacgccgca
     2461 ttaatgcctg cattcgcgcc atcacctctt caatatgaaa cggtttagtc acgtaatcat
     2521 cagcaccggc acttaatact tcgactttgt cctgccagct ttcacgggcg gttaatacca
     2581 gaatcggcag tgaaacatcg ttgctacgcc agcggcgaat cagtgacaga ccgtcctcgt
     2641 caggcaatcc gagatcgaca atcgcaatat ccggtaaatg ttcattgaga taataatcgg
     2701 cttctttggc atcttctgcg tcatcgacct gatgaccagc atcctgaatc tgaactttaa
     2761 ggtggtgacg taacaacgca ttgtcttcaa caaccagtac gcgcattttt atttctccct
     2821 gtcttaatta ttaaaatagt gtaacgcgat tatgtgttta tgggggtaaa cattaaataa
     2881 accagcgggg aggggaggta aagtgaaaaa agaaaaagtg gataatctta ataagcaggc
     2941 cggacagcat cgccatccgg cactgatttg aggcttattt cagctcatca accatcgtga
     3001 tagctcgacc aatatagtta gccggcgtca tcgctttcag gcgggctttc tcttcttctg
     3061 gcaacgccag accatcgata aactgcttca tgccttcggc gtcaacgcgc ttaccgcgag
     3121 taagctcttt cagcttctcg tacggttttt cgatgccata gcgacgcata actgtctgga
     3181 ttggttctgc cagcacttcc cagttgtgat ccagctcatc cagcagatgg tcacggttca
     3241 cttccagttt gctcacgcct ttcagggtgg attgatacgc aatcagcgca taaccgatac
     3301 ccacgccgag gttacgcagc acggtggagt cggtcaggtc acgctgccag cgggaaaccg
     3361 gcagtttgct tgccagatgc tgcaataccg cgttggaaag gcccagattc ccttcggagt
     3421 tttcgaagtc gatcgggtta actttatgcg gcatggtgga agaaccaatc tcaccagcaa
     3481 tggttttctg tttgaagtgg ttaagggcga tataacccca gacgtcacgg tcaaagtcga
     3541 tcagaatggt gttgaagcgc gcaacgcaat caaacagttc ggcaatgtag tcgtgcggtt
     3601 caatctgggt agtgtacggg ttccattgaa tacccagcga ggtgacgaac tcttcgctga
     3661 actgatgcca gtcaacttcc gggtaagcgg cgatgtgggc gttatagtta ccgaccgcgc
     3721 cgttgatttt gccgaggatc tccacctggt taagctggcg gtgctggcgc tccatacggt
     3781 aggcgacgtt tgccatctct ttaccgatgg ttgacggcgt ggctggctga ccgtgggtac
     3841 gagacagcag cgggatatcg cggtactgaa cggcgagatc tttaatgcca tcaatcagtt
     3901 gacgccagta cggcaggatc acttcatcac gcgcggtttt cagcattaat gcgtgggaga
     3961 ggttattgat atcttccgaa gtacaggcaa agtggatgaa ttcagaaacc gcgtgcagtt
     4021 ccgggatctc cgccactttt tctttcagga aatactcaac cgctttaacg tcgtggttag
     4081 tggtacgctc gatggttttg atgcgtgcgg catcttcttc gctgaaattg gcgacaattg
     4141 catcaaggta accgattgcg tcggcagcaa aagcaggaac ttccttgatc gctgcgtgcg
     4201 cggccagttt ttgcagccaa cgtacttcaa cttgtacacg gaatttcagc aaaccatatt
     4261 cgctgaaaat cccgcgcagc gcgctgagtt tatcgccgta gcgtccatcg acaggggaaa
     4321 cggcggtcag tgaggataat tccatagatc acaactccgg ggttaaatga gcaagaattt
     4381 gttttgcctg agtggtcagg cgattacgag aaaacatcag ttgtagacgt ccgccgccga
     4441 cctggtgcca gagcacggcg gcgcgaatgc ctgccagcag ggttgcgcga actttcgcct
     4501 gcacttgtgg gctttgcagt acagcagggg aaccggtgac ctgaatgcgc gggccaagcg
     4561 ggctgatcac atcaacatag atagcagcca tcgcgctcat cagcgtttcg gactgtaaat
     4621 cgaagtgttc gagctggcgt tgcaggccgt tgatccggtt gcccagagtg tcgagcgcgc
     4681 ctttcgctga ggagagtttg cgctcaagca ccatcaagct gagtgtgtag cgggttaatt
     4741 cggcgtttaa gccctggcaa ctgctggcat tgagcacgcc gagcagggtt tccagcccga
     4801 cgcgcaggtt ggcttcgcta ccgccaaaaa ccgccagcgt cgagctgggg ttcatatcaa
     4861 taatactgtt gagtgagacg tgtagcgcat cggcatcaca atgcccctgg tgagcgagtt
     4921 gttgcaccag gcgtgccgac tgacaaatac cggccagggc gagggtgatg tcatagtaat
     4981 tctttgccac gttcactgct tccttgtttt aagtaaagat aataatcaga ccggcagcgg
     5041 cagacgctgc tcaataatac cgccaccgag gcacacttca ccgttataga agacggcaga
     5101 ctggcccggc gtcacggcgg caaccggctc atcgaaaatc actttaatgc gatcgtcgtc
     5161 cagcgccttg acggtgcacg gaatgtcggt ctggcgatag cgggttttta ccgtgcaacg
     5221 catagtgccg gtgaatggtt cgcgatcgac ccagtgcagc tgctgggcaa tcaacccgac
     5281 agacatcagc cgcgggtgtt catggccctg agcgacaacc agaatgttgt tttcgacgtc
     5341 tttgtccacc acataccacg gttcttcggt gccttctttg gtgccaccga tacccagacc
     5401 tttacgctga ccgagagtgt gatacatcag cccctggtgc tcaccaattt catggccatc
     5461 gacggtaatg attttgcccg gttgcgccgg gagataacgg cccaggaact cgcggaattt
     5521 acgttcgcca atgaagcaaa tgccggtaga gtcttttttc ttcgcggtga ccagaccaag
     5581 atcttcagca atcttacgca cctgcggttt ttccagttcg ccgaccggga acaggctttg
     5641 cgcaatctgc tcatggctga gcgtataaag gaagtagctc tggtctttat tgctgtccag
     5701 accacgcagc aggcggctct tgccatcgac atcggcccga cgtacgtaat gaccggtagc
     5761 gatataatcg gcacctaaat cttcggcggc aaattcgagg aaggctttaa atttgatctc
     5821 cttgttgcac agaatatccg gattcggcgt gcgaccggct ttatattcgg caaggaacag
     5881 ttcgaagacg ttgtcccagt actcggcagc aaagttaacg gtgtgcagtt caatgccgag
     5941 cttgtcgcag accgcctggg catcagccag atccgctgcc gctgtgcaat attcctcacc
     6001 gtcgtcttct tcccagttct tcataaacag gccttcgacc tgatatccct gttgttgcaa
     6061 cagccaagca gaaacggagg aatcgacacc gccggacatg ccgacgatta ctttttttgc
     6121 ggtttcagac attggatcac tcacgacatt gaacttcaag gcggcgtatt ctatcacgca
     6181 gccttttaga tgacaccctt tgtaaaaggc cagttaaaat cgccaatcat ctccagcgga
     6241 taacgttgcc cgctttgata acaacgaata ctttccgcca ccagcggcga acgaagattt
     6301 gacgcctgta aaatttcttc ggcgctgacc caacggcagc agtcgatatc gctgtcatga
     6361 ggctgggtcg ggcatatttg ctcaagctca atggcaaaga ggaaacgcaa aaacggcgtt
     6421 ttatctggcg caatccactg atgcatacga ataaagtgtt gcggctgcgc gctgatgccg
     6481 gtttcttccc acagttcacg ggcggcggct tccactaagg tttcatcggc ttccagatgc
     6541 ccggcaggtt ggttccataa cgctttacca ttaatcgtct cttcaacgac taaaaatttg
     6601 ccttctgcgt gcaccacgca agcaacggta acgtgcggtt taaacatttt gcatccttaa
     6661 tctgtcactt ctcgccattc gccattggca agattatcca aagagtaatc tcccatcgca
     6721 tagcgaatca gtcgcagcgt ggggaaacca acatgggcgg tcatgcggcg cacctggcga
     6781 ttacgtcctt catataaggt gatcttcagc cagctggtgg gaatactttt gcgttcacga
     6841 attggtggat tccgcggcca taaccacgcg ggttcgtcaa ccagctccgc gcctgcgggc
     6901 agggtagggc catcatttaa ggttacgcca ttgcgcaagg cttcaagtgc gtcttgtgtg
     6961 ggaatacctt ccacctgcac ataatagatt tttccggtgc gtttacccgg ctgggttaaa
     7021 cgcgcctgca acgcgccgtt attggtcagc accagcaacc cttcgctatc gcggtcaagg
     7081 cgacctgctg cataaacgcc ctgaaccggg atgaattctt ttaatgtttt gcgtccggct
     7141 tcatcggtga actgcggaag aacatcgtag ggtttattga agaggatcac acgcgtgggc
     7201 tggttttcat gtttacgcct ggtagaacgt tgcgagctga atcgcttaac ctggtgattt
     7261 ctaaaagaag ttttttgcat ggtattttca gagattatga attgccgcat tatagcctaa
     7321 taacgcgcat ctttcatgac ggcaaacaat agggtagtat tgacaagtca attacaaatc
     7381 attaacaaaa aattgctcta aagcatccgt atcgcaggac gcaaacgcat atgcaacgtg
     7441 gtggcagacg agcaaaccag tagcgctcga aggagaggtg aatggaaagt aaagtagttg
     7501 ttccggcaca aggcaagaag atcaccctgc aaaacggcaa actcaacgtt cctgaaaatc
     7561 cgattatccc ttacattgaa ggtgatggaa tcggtgtaga tgtaacccca gccatgctga
     7621 aagtggtcga cgctgcagtc gagaaagcct ataaaggcga gcgtaaaatc tcctggatgg
     7681 aaatttacac cggtgaaaaa tccacacagg tttatggtca ggatgtctgg ctgcctgctg
     7741 aaacccttga tctgattcgt gaatatcgag ttgccattaa aggcccgctg accactccgg
     7801 ttggtggcgg tattcgttct ctcaacgttg ccctgcgtca ggaactggat ctctacatct
     7861 gcctgcgtcc ggtacgttac tatcagggca ctccaagccc ggttaaacac cctgaactga
     7921 ccgatatggt tatcttccgt gaaaactcgg aagacattta tgcgggtatc gaatggaaag
     7981 ctgactctgc cgacgccgag aaagtgatta aattcctgcg tgaagagatg ggcgtgaaga
     8041 aaattcgctt cccggaacat tgcggtatcg gtattaagcc gtgttctgaa gaaggcacca
     8101 aacgtctggt tcgtgcagcg atcgaatacg caattgctaa cgatcgtgac tctgtgaccc
     8161 tggtgcacaa aggcaacatc atgaagttca ccgaaggcgc gtttaaagac tggggctacc
     8221 agctggcgcg tgaagaattt ggcggtgaac tgatcgacgg cggcccgtgg ctgaaagtta
     8281 aaaacccgaa taccggcaaa gagatcgtca taaaagacgt gattgctgat gcattcctgc
     8341 aacagatcct gctgcgtccg gctgaatatg atgttatcgc ctgtatgaac ctgaacggtg
     8401 actacatttc tgacgctctg gcagcgcagg ttggcggtat cggtatcgcc cctggagcaa
     8461 acatcggtga cgaatgcgcc ctgtttgaag ccacccacgg tactgcgccg aaatacgccg
     8521 gtcaggacaa agtaaaccct ggctctatta ttctctccgc tgagatgatg ttacgccata
     8581 tgggctggac tgaagccgca gacctgattg ttaaaggtat ggaaggcgcg atcaacgcca
     8641 agaccgtaac ctatgacttc gagcgtctga tggaaggcgc taaactgctg aaatgttcag
     8701 agtttggtga tgcgatcatc aagaatatgt aatcactaca tgtgttaaat atttaaacgg
     8761 gcgtataaca cgcccgttgt tttatttatg tggatattat taatagcata tcgagcatat
     8821 ttatataaaa cctattactt gagcccatat gggcatattt ttataataca actcttatgc
     8881 aaacatttat ttgttatttc gctttctcct ggaggacact cttgactgct tttgagtaaa
     8941 ctccataaat tcttgttgaa tggtgcaatg ttataaatag taataggata ttctttttcc
     9001 ttaaggataa taccaagctt aaccggtgta aatatccttc caggaggaag aaatatagta
     9061 gattgatacc agatgatcat tttcatatca ccccatatgg ctgaaaaaga tatgccgcat
     9121 gtaggttgaa ttaccgtgtc aattactacc cacttcattt gttatgtctt atcccacggt
     9181 atttaatata gttcatttgg atgttcattt ctttattttg catatgagta tattacccct
     9241 tcaaaaaata aattaattaa aacgattgct tatataaaac aaaatttaaa tcaaggaatc
     9301 tcaatggatg ttaaacaaaa tgagatttag tgaaaacaat aaattattca cttcgtttta
     9361 gatttgttta gctataatgt tatacattca aatgactgaa catcctgtaa ttaaaacata
     9421 gcctttatgc tacttggtgc caatttgcta aacattatgg ttgccttttt atataacgat
     9481 aataatgaat ataaacatga catgagaata aggtttcaat ttttgagtta tataggaaag
     9541 gtttaacctg ttcctggcta aaatacatat aactggatga tgactaaacc aaaacacata
     9601 tgcgttaagt attgaaacgg gcgtatggca cacccgttgt ttttataaat atattaaccg
     9661 ttataaaata acgtataaaa agtcaagtga tcacatttca aatatcaatt tgatagtatg
     9721 ggcttggtga ttatttatgg gtagcaataa aaggacagta tttatcatcc atagggatag
     9781 tctctgtact tttattccca ttatgctaat gccttactga attatgaagc atttcttaag
     9841 catccaactt tagctagatt aatggtttat tattttctac atcttcaata tataaaagcg
     9901 tattatcaat ggcgtagtaa ctgcgtttgt tatgattaac atcagtaacc caacggaaaa
     9961 cgcccgcgcc tgctagtgtt gaacagtatt cccgaaatgt agattttccg caaatatgaa
    10021 gcaatgcggc ctcttttatt ttagcaggat tcttggtcgt actaactttt aactggttcc
    10081 tggttcctct taataacaaa accgtgtcat cgtgagtaat aattctgatg ttatccgtag
    10141 ccagataata aatgtaatgt gcaatacggt gatgttttaa ttctgaataa aaccaggaga
    10201 agttttgctc ttttctcact tgctcaaaca tcttttgaaa aacaacgacc tgatccatca
    10261 ggataataac ctcttgttag ttgtgagact gcgtagtgtg cacgatcggt tttaccactt
    10321 caatctggtc tgtcctttgg ctgtgatatg tacagagtgt gatagaggga ttacctgaat
    10381 tctcccggtg agcattttgc aacggaccag ctccggtaca aatgctgttg tgggttcaga
    10441 ttataacatt ctgtctaagg ggcgggataa aggtgaaatt agggggcatg aaagatgact
    10501 ttataacctt gctcacccca atgttgtaaa agttcgtttt gccttctcgt tggtgccatg
    10561 cctgtccaga caatcaatgt ttgcgtcggg aacagttccg ggcgcggcga ttcaatggga
    10621 tcagcaagaa cagaaatgtg ccatcctgac aatgataaac gccatgcttc cagccacaat
    10681 cgtgctctct cttcaacatt ccacgccatc agaatggctt ctttaccggg cttacggcgc
    10741 gtttcgaaaa gcgaggttgc tgcgtattca attaatgcgc cgtcaaacat actgctcata
    10801 atgcgggagg tgttgtgatc aagcacgaga cgctggcgaa caggaaggta aacatgatta
    10861 atcaattgat ctactgggta ctctcgaccc agtgaaataa ttctcgcgcg tagtttggca
    10921 ggattagcca tacgaagaat tgacatcatc tcttcttgca ggcggctcca gtcatcttcc
    10981 gtatcctggc tggtggtttc cagtaatgct ttaactttgc ctacagggac gccattactg
    11041 atccaacgct tgatctcttc gatgcgttgt atgtcttctt catcaaagag tcggtgtccg
    11101 ccttcactgc gctgtggttt taacaaaccg tagtggcgtt gccaggcccg gagagtgaca
    11161 ggattaatcc cgcaacgttc agcaacatca ccaatgctgt aataagccac aattcctcct
    11221 tgcggtcaca aatctccgtc gccggtacac gacccaataa tactttgtac aatatacgct
    11281 aaaattgtac aaagtataaa taagatttca gctaaattgg atgaaacatt atttttaatg
    11341 tagattaaat ttaaacataa cgtattcatt ttcacgatga tttactgaaa tcatgtgaaa
    11401 gaatgtgctg aaaattattt tttctctggc cacgctatgg aagggatacc attcaattta
    11461 gctttagcaa acagatctcc ctgaaacatc tcaattcctg cggattcaag ccacatccac
    11521 tcttctggtg ttgccacgcc catagcactg acttgaattt caagtgatgt acagcatttt
    11581 atgatcgctt gaataattgc ctgccgtggc ccgcttttat gaacattggt aatcaattcc
    11641 tgactgattt taattctgtc aggctggaag cgtgacagga gtaacaaacc ggcaaaacct
    11701 gcgccaaaat gatcaattgc tacactgata ccggcagcct taagcgattt aatcgcttct
    11761 gcaaactcat caaaccgaga tataacttca ctttcagtaa attcaacgat gatttgttca
    11821 ggcaccagag cattggcctt tatttcatca agtaaaaaag agactgcgtc aggttcgtta
    11881 accagggtca taggtaatag attgattgaa atcattttat caccgagctc aagtgcgtgt
    11941 gtcatcgcga atgcaagcgc cttacttttg agatccgctg tgtagatttc cccgtcttta
    12001 cgctgcccaa ccgctatggc tgatgggcta tcttcatttt tttgcacaat ggcttcaaaa
    12061 gcgattatcc gccgcgataa gggatcgaca ataggatgaa aggcaaagtg gtcgtttata
    12121 gttggtgaaa gggtacagga atcaagttct ttatcagatc cgtcagcgat aaaaagccag
    12181 gagtcttcgg cagggatctc gaaataggtt gattgttcgg ttgcaaggac aaaagtacga
    12241 aaaaattgta gcgctctgtc atcataagtt agctgaaatt ttgatgtgcc tttgtcgaat
    12301 acggcctgta aaacgtcatc tcgctcgtgc aggcgcaaat caaataattc cattcccgct
    12361 ttgccaaaac ggcgagcagg cgcgtaatcg cacagcagct caacaatatt atagtgccgt
    12421 ggatcttggc atatagcccg atatatcatt ttaacctgtt cttccggacc ttccagaagc
    12481 tggaaaaaat gagaaccatt aaacagtaag atccctgtta cgtcagactg catgttcctg
    12541 cgatttgcta tcgaaaccat ttcttcgatt tttttgacag gttcgtcgtc acgtatatgg
    12601 ctacgataaa taagggtggt aagcattaac aatccagggt aatgggtgag gcgagagtaa
    12661 gacggtaaca gacatatctt cttgtgtctt tcttttaata ccaaaacata accgtttctt
    12721 tacattgata aaaaatggaa aaagttgaac acgagttggc gaaaaatctt gtatagattg
    12781 tcagttaaat gatgcaatat gttttatcat aacacattgt tttatataca ttagtactaa
    12841 ttgcaaaaaa ttaatttatc attctgtaca catatttcgt acaagtttgc tattgttacc
    12901 tcacttcaca ttgattaaca tttttaacag aggcgtagca tgcatcaaaa ttcagtgact
    12961 tcagattctg cgggagcaat cactcgttac tttgcaaaag ctaacttgcc tactcagcag
    13021 gaaactctcg gggaaattgt gactgagatt ttgaaagatg gacgtaatct gagtcgaaag
    13081 tcgcattgtg ccaaacttct ttgccgactg gaacaagcga caggggaaga ggaacagaaa
    13141 cattataatg cactaattgg gttgcttttt gaataatgca acagcctgat tatttaacag
    13201 gttagctatg aagtcgttat gaagacatct gataatgaac gtataaaata tgaaattact
    13261 ggccaggcgg tgctccagat actgcgcatg aagataaatt tttcattgca gactcttatt
    13321 aagcaattac tcgtaatgaa atcggctgag gaagatgctt tccgacgcga tttaattgac
    13381 agcataattc gtgattttag caatagtgat tcaggagggc caaaccgaag aacagcgaca
    13441 gctgacaata aaagtatgtt caatggtaag aaaataaaca gaatacatta aaatttcata
    13501 agtaagatga gaggttacca tgcttgaaga tactacaatt cataatgcaa taactgataa
    13561 agcgttagca agttactttc gcagttcggg taatttgtta gaagaagagt cagcagtgtt
    13621 agggcaggct gtcaccaatt taatgctttc aggcgataat gttaataata aaaatattat
    13681 cttaagtctg atacactccc tggaaacaac aagtgatatt ctcaaagctg atgtgattag
    13741 aaaaacactg gaaatcgtgt tgcgatacac agctgatgat atgtaacttc taattattaa
    13801 gtataagttt atagaaaact cattcatctt atttttgtct gtcgctttag actatacaca
    13861 gcataatttt attgggttaa tatttctacg aggctgacat gaataattca atcccagaga
    13921 gatttatttt tcaatgtgct ttgtttaaaa atctcgagag ggaggtgttc atgacgcatg
    13981 ggtatgttga tagtcatatt attgatcagg ctttacgctt gcgtttaaaa gatgaaacca
    14041 gtgtgattct ttctgatctc tatctgcaaa tattgcagta cattgaaatg cataagacta
    14101 cactaacgga tatcattatt aatgacaggg aatccgtgct ctcttagaac ttgagtaagg
    14161 ttttagaata aacatcagct gtatcaccat gctgatgcaa agtgagcatt tcaggcgtta
    14221 tgctttctta ttatgtccgc aatatcaggt gtcaagaatg gagagttctc gctctccatt
    14281 cttgacgcct gatatcccgc ctaacttatt ttgtactgcc tacattatgc tcaataatta
    14341 atgggttaga tcaagatgga gaaaatttta tcttcggcgt ggatatttct tatgctctct
    14401 accatgcatc tctgtagatg ccagcgcgag cgaatgaatg gcattt
//
LOCUS       DAJTYF010000100        14252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTYF010000100 DAJTYF010000000
VERSION     DAJTYF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.100"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..106)
                     /locus_tag="OO590_003083"
     CDS             complement(<1..106)
                     /locus_tag="OO590_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR6638371.1"
                     /translation="MKLLKVAAIAAVVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="OO590_003084"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="OO590_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6638372.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="OO590_003085"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="OO590_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6638373.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="OO590_003086"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="OO590_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6638374.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            2425..2841
                     /gene="csgF"
                     /locus_tag="OO590_003087"
     CDS             2425..2841
                     /gene="csgF"
                     /locus_tag="OO590_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6638375.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSVLQNNSTDF"
     gene            2868..3643
                     /locus_tag="OO590_003088"
                     /pseudo
     CDS             2868..3643
                     /locus_tag="OO590_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3707..4198)
                     /locus_tag="OO590_003089"
     CDS             complement(3707..4198)
                     /locus_tag="OO590_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6638376.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="OO590_003090"
     CDS             complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="OO590_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6638377.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="OO590_003091"
     CDS             complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="OO590_003091"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6638378.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="OO590_003092"
                     /pseudo
     CDS             complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="OO590_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6842..6929
                     /locus_tag="OO590_003093"
     tRNA            6842..6929
                     /locus_tag="OO590_003093"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6876..6878,aa:Ser,seq:gga)
     gene            complement(7079..7360)
                     /locus_tag="OO590_003094"
                     /pseudo
     CDS             complement(7079..7360)
                     /locus_tag="OO590_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7642..8646)
                     /locus_tag="OO590_003095"
     CDS             complement(7642..8646)
                     /locus_tag="OO590_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR6638379.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8718..9278)
                     /locus_tag="OO590_003096"
                     /pseudo
     CDS             complement(8718..9278)
                     /locus_tag="OO590_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9363..9560)
                     /locus_tag="OO590_003097"
     CDS             complement(9363..9560)
                     /locus_tag="OO590_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638380.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9572..10063)
                     /locus_tag="OO590_003098"
     CDS             complement(9572..10063)
                     /locus_tag="OO590_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638381.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10060..10434)
                     /locus_tag="OO590_003099"
     CDS             complement(10060..10434)
                     /locus_tag="OO590_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6638382.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10481..10855)
                     /locus_tag="OO590_003100"
     CDS             complement(10481..10855)
                     /locus_tag="OO590_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR6638383.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11018..11239)
                     /locus_tag="OO590_003101"
     CDS             complement(11018..11239)
                     /locus_tag="OO590_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6638384.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11302..11778)
                     /locus_tag="OO590_003102"
     CDS             complement(11302..11778)
                     /locus_tag="OO590_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6638385.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11794..12267)
                     /locus_tag="OO590_003103"
     CDS             complement(11794..12267)
                     /locus_tag="OO590_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6638386.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12609..13430)
                     /locus_tag="OO590_003104"
     CDS             complement(12609..13430)
                     /locus_tag="OO590_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6638387.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13530..13703)
                     /locus_tag="OO590_003105"
     CDS             complement(13530..13703)
                     /locus_tag="OO590_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR6638388.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13823..>14252)
                     /locus_tag="OO590_003106"
     CDS             complement(13823..>14252)
                     /locus_tag="OO590_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638389.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3641 a         3495 c         3409 g         3707 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gactgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaattgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagct tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatatttttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatattt aacacttgat ttaagatttg taatggctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     2641 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     2701 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     2761 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc ggttttacaa
     2821 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     2881 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     2941 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     3001 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     3061 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccagggct tacaaaacct
     3121 gcttaacgag cgcaaggtta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa
     3181 ccgaatcccg ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattattgg
     3241 ttatgaaagc aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga
     3301 cacgcaatac cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg
     3361 tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg
     3421 ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa
     3481 cgaacccgtt atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa
     3541 tgatggtatc gaccgtggtc tgtgggattt gcaaaataaa gcagaacggc agaatgacat
     3601 tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga
     3661 attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt
     3721 tgatttcctg ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt
     3781 ttttcattgc gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt
     3841 caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca
     3901 gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga
     3961 gaatttccag atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc
     4021 cactaagcag ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa
     4081 agtaggccgt acagcccagg aagcccgccc agcttagtaa acccagggaa acagccaccc
     4141 atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc
     4201 cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa
     4261 caaattatct gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc
     4321 cagctcgtcc cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa
     4381 aggcgtggtt gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata
     4441 ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca
     4501 ggaagccgca agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtggcatccc
     4561 tcgttcggaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg
     4621 atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg
     4681 cgtcatatca cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt
     4741 cgcagccagt ttcccctcac gaatcagagt aaacagcggc accagtaaag gatcttgcgg
     4801 ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat
     4861 atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat
     4921 tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctgg cggaaaatct
     4981 accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa
     5041 tccgagccta atgccaccca acctccagca tcacgtaccg ctgcagctac cgcacgacag
     5101 ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc
     5161 tgatgtttcg cggctgcttc agcaacggct ttaacatcta tttcatattt cggatttccg
     5221 ggatggctta ttatatgcac attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg
     5281 gtcgctttgt catgtggcgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc
     5341 gagtcaaaca ttttaccgct gcagtcaatt tcaccatcaa catttttaat gttagcttca
     5401 atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag
     5461 tgccagtgat gcggcgcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta
     5521 atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg
     5581 gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg
     5641 ccaaggatag caggaatcct gatgctttat tagtagccgc gtgcgcggtc gacttgcccg
     5701 cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca
     5761 gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc
     5821 ggactttcag gcggtaaggg ttcacgatta aaaacatcca gcattgcgcc tttaactttc
     5881 ccgctatcca gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg
     5941 aggagatacg cgccatcagg taatttttcg agtaattgtt gattaataat gccgacggtt
     6001 tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt
     6061 tcttcccgtc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa
     6121 cgcagcggaa agcgctaggt ttgcagactc tgagcaactt tacagcccag tacgcctgcg
     6181 cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa
     6241 ctattttgct ggatgcgata atcatcaaaa cgtcgaaacc aatgcagcac ctgactgaca
     6301 gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa
     6361 gggttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg
     6421 agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact
     6481 aaaacataat cagcagagtc attatctccg cttttccatg ccctgactct tgcctgagga
     6541 atagctttgc gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg
     6601 atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat
     6661 ctacaaaggc caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt
     6721 attaaaacca cacattgatt gaagtttgaa taaacgcgcg attctttcaa aaagtttgtt
     6781 gacctcaggt catgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt
     6841 tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat
     6901 cgggggttcg aatccccccc tcaccgccag attataagtg catgttattg tttaacatgc
     6961 gcttttttat ttttccttcc agacagtatg ccatcgggta tgccatctga aaccggttta
     7021 tatgccccgc gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct
     7081 atactgccag catatcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt
     7141 ctggttttct gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct
     7201 catcttcata gctctcttta ccctgaacag actgaatgtg atcatccaga cgaagggtga
     7261 tgtttccccg gttgtcgggg attggtttac cgctgaacag gtgcaacatt ctttccagat
     7321 cggcgagtcg gtcagcctgt tgtccggtga taagatgaaa tctatggtca cccccgtttt
     7381 tgcaacactg atttttgatt aatggttggc ttgcttaaat ctatccggcg tctctgtggg
     7441 caaggatgcc cgcgccccga tgagttccgc acctgataat cctaaaaatc ccctcggctt
     7501 cagtgagcca tttcatctgt taggttttta atcaggtcga tttgccgttc atgtcatcaa
     7561 taatcagtct tcgcaaaacc agaaaggtaa cttgtatctt attgtttaaa ataaactaat
     7621 tgagttatat cagcatctat atcaggcctg atattctttt ttctgagtag tgattgccca
     7681 ggcaattcga gccagtttat ttgccagagc gcaggtaacc acattcgaat gcttcttgtt
     7741 gagttgctcc cgaacccact cagcaagttt tccatgcttg cgttcaagtc gcattatgaa
     7801 tgagcgggca cactgaacca gtaaccggcg taaattttta tcacctcgct tactgatacc
     7861 caacagagtc gattttcctc cagtgctata ctgccgggga acaagtcctg tggaagctgc
     7921 aaaatccctg ctgcatgaga actgtttccc gtcaccaagt tgcgacgata gtaagctggc
     7981 agtaatcgga ccaacaccag gaattgtcat aatgcgctga gcggtatcat cagcgttgat
     8041 tgactgactt aattctgatt ccagctctgc tatttgctca acaaggtaaa agtagtgggt
     8101 atgcaacctc accaaaaggc ggctcagata ttcggggatt tcgtgttcag ccaacacgag
     8161 agacaaccgt tttattacag catcacctat cggcaggctg attccaaatt ccagcaagaa
     8221 accgtggatc tggttggttg ttttcacctt atctctgatg agcgattctc tgacccggtg
     8281 taacgccctc atggcctgct gggtctcagt tcttggctgg acaaaacgca ttgatggtcg
     8341 tgaagccgct tcgcatatgg cttctgcatc aacaaaatca ttcttattac ttttgacaaa
     8401 cgggcgaacg aactgtggag aaatcagctt agtttcatgc ccaaaatcag agatacagcg
     8461 agccataaag tgtgcacccg cacaggcttc cattacaaca actgagggcg gacatgtcgc
     8521 cagaaactgc atcagtttag tacgggtaaa ttttttacgc agaagagcct taccagactt
     8581 gtcttgacaa tgaatgtgga aggagtgctt tccgagatcg ataccaataa gtgtgatgtt
     8641 ctgcatgatg gtctctccgt taaagggatt taccttaaca gcgtactgct gttaaggtgg
     8701 gggtgaccat atcattatcc ccaccggtcc catctcacca gattgttgac gataatttta
     8761 ctgccaaatt tacaggggca attggttttg taattccagt tcagccctct gaagacgttg
     8821 atcacccctc gctcaaacac ctcatcctta ttctggtgca gttgttcaaa tgtgctcaga
     8881 atgttcgctt cgctgatttc cggaaaatta tcgtactcca gtgaccggta ccatgtatca
     8941 cgggcctgcg tatccattaa tgacagcatc ccggaacgtt gcatcaggtc acgccagatt
     9001 tcgcgatcaa gattccgggt aatggctttc attgctgttt cgggtttctc catcagccag
     9061 cagccgcagc gaaaatcctg tttcatggcc cagtccgggg cagttttacc tccgatgctt
     9121 tccgtcagca ccgagatttc agccagttgt cggataagtg tgtcgatttg caacagtgta
     9181 ctctttcgta cagccagaat gtggcgaatg ctggttgagc agataacgtc cgtatgcccg
     9241 ttgaccactt ccggacggga gattgtgata tctgacataa tcgatattcc tttttgcaga
     9301 taaatgaaaa cgccagccct gtgcaggctg gcgtcagagg aatgaagtct ggtgagtgag
     9361 cttcaccggt tctggtgcag aagctgcaga tgacgcaggg tacggacatc ctcccgggcc
     9421 tgttcgatac agggcaacag tgctgctgaa tccgttttat cctcatcgtt gtcaaagata
     9481 attcccgatc cgcagtcgat attgtcctgc agccacgcaa tcagaatatc cagcgctgtt
     9541 tccgtggtta atgatttcat gttgttcctg gttaccagtc gaaagtgggt aaacccggca
     9601 ggcattcagc gctggcatcc agatgaatga gactgacgcc ataacgccgg atgagtgtga
     9661 cgaccagacg acggaacgtt tttgacagcc ccaggcgttt aagccgcaga accgggtatg
     9721 accacgcatc ggtacgtaac agatatccgc tacctgtaaa atgaatccat tctgattcac
     9781 cgaagtcact ggtctggtgt gacagcgagt acaaccaggc gttgtccgtt tccgtgatat
     9841 gtgcggtact gcagcgtatg ccggtaaggg tagcaaatgg tggcgtgcat tctgctgagg
     9901 tctggtcaga ttcatcaaca acacgaaggg agtagccgtt atcgcagacc atgttaatca
     9961 gttcagcgag ctcaatacca tcgacgtcaa cgacaatgcg ccctatgttc agtgcctgta
    10021 cgttaacgct gtcggcttcc agtgtcaggg ccagtttcat catttcgcct ccggatactt
    10081 acccagggta atgttattta ccgttctgta attgtcgcgg gtcatcaggc cggtcgcccg
    10141 gcgagcccgg aggatatcaa tactgtttat taactgagag cggggacagg tgctgaatcc
    10201 cggctggtcg gtacgcacca gcgcgtattt ttccacgaga aaattaactg catcacacag
    10261 tgaaatgcct gcctcaatat gctgctcaat cacacgttca tcgacaaacg gtgtgtcatt
    10321 cagtgtgagg ccataatgct ggtccagcag tcggatgagc agtgtctgcc agatggtgac
    10381 gggagacggg cagcgagata cctcccgtac atgtgtgtcg gataatgttt tcatcttaag
    10441 ttctctgtaa ctggtagtgc taaaggtgag agcttcgggg ttatgaggtg gttgcgggtg
    10501 ctggtgtcgg ataaacagcc agataaacgt aaccacagga gccgagggtg tcggcttcgc
    10561 aggtcagccc ttttgcatac agcgtgacgg tatgctgata tcggggattc agttcaccgc
    10621 tggtgagcat gagttccagt tgtttcatca gcagcggaaa ggcctggtcc agatggtatg
    10681 catccgcatt gctgaaccgg cctctgatac cggcgcggtc tgcaaggtaa tacaggcggt
    10741 taccctcttg caccagacga gcgccaaaac agggcctcac tgtgcaggga agcccccacc
    10801 aggggcggtc gtgattgtca tcgggatgag ttatcccgga gagtttgtct gacacgataa
    10861 aatcctctct gaaaagtggt taagaatggt ttaacgatga caggtcagcc ggcgcgctga
    10921 ccggtttcgg tgaagatgta tgcattgatg agaatggcct catccacagc ggcgtctgag
    10981 gtcatctggt cgtactcgtt tcccgggtga cggaacatca gttcagggtg acactcatca
    11041 gccggacata cgggccaaaa ctgtccttac ggcgttctgc aaacacggca agaacgccgg
    11101 gaatatcctg cacttcacga ccagtatacg cttcagcact gccgtgccag cgatacttac
    11161 cggtacagaa cggaaaaaga cgggatgcag gatgctgttg gtgaatacgc atggcttcac
    11221 cacgggtgat gattctcatg atgtgatacc tctgaagaca gaagataaaa gtgaaaacag
    11281 gtgtgatgtg gttgtgacgg gttaaagcag accatgttcg gcaaaggaga aaacctggtt
    11341 gccaccgact atcagatggt ccggcacccg gatatccacc aggcccagtg cctgtaccag
    11401 acgttcggtg ataagccggt ctgccttact gggtgtgact tcaccggacg ggtgattgtg
    11461 cgccagcacc acggcagcgg cattgtggta cagggcgcgt ttaatcactt cccggggatg
    11521 aacttccgta cggttgatgg tgccggtgaa gagggtttca ccggcaatca gctgattctg
    11581 gttgttcaga tacagcaccc ggaactcttc ccgctccagt cccgccatgt tcagaatcag
    11641 ccattcccgt gccgcacggg tggaggtgaa ggccacgccg ggttcatgaa gatggcggtc
    11701 cagggtttgc agggcccgca gaatgagact gcgctcgccg ggcgtcatgt ttccgggcag
    11761 aaaggaaagc tgctgcattg tccctgcctc cattcagtcg atgatgcgca taatggcgct
    11821 gcattccgga tgctgcaggg cgtaatcccg cagccggtaa taatggaccg tcatggcata
    11881 acactccgta cgacaggcat gatgactgta cgtcatcaga caggcagcaa tgccggcggc
    11941 ttccgggctc atttcagcgc ggttaccgtt catggcattg aacagtaccc atgtttcgtc
    12001 atcatcgtca tccggttcgg gtgccataaa tgccccgccg ttgttcaggg tgtacagatt
    12061 ccagatacca ccgcagtagt cttcacacag acggtccatc cagccgaaga cacggggttc
    12121 cagggtgacc cactgtggaa tgaggccaaa atgctgcggc cagaagctga tgcgctgttc
    12181 atcagggacg ggagtggcaa ccagttgttg caggccagtc tccggtgcac aggccgaagc
    12241 ggtggtgttc tgagacaatg ttttcatgta tgaaatcctt gtaatgtgaa taaatgagag
    12301 ggagtaaagg tcgggagctg gcaacatcac gttcatcact gaccgtgatg acccggtaat
    12361 atcagggatg aatgatattc ggctgaaatt ccggacagcc gtacgcgtaa ccgttccggt
    12421 cacagacgga tggccagatg ccattgcctg tatactcagt ctgcacagac tgatttctcc
    12481 gggacggtaa agcagacggg tacaccaggc gcacaggtca tcatcagaaa tggaacgaca
    12541 ccccggcagc gcctccgggg cgtcgggtga ttctggtgcc tgaatcacgc cttcagggac
    12601 atgctgcctc agcgcaggct ctccagcagc gtttctgcca ttacccacaa tgcgcggttg
    12661 agcttaatat cggtgtcgat gttgtgaatg gcacgggtgt ggatacgttt tccttttgca
    12721 ctgcggccgg aaatcccgcc tttcagcata ttctcctgga tggtctgata tgcgctccac
    12781 aggtctttac cgtaatcctc cctgcgtcgt ggtgtcagaa tgtcagcggt ggtgactggc
    12841 tgatgttcgt caccataacg gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt
    12901 ggcggcagga ccagcgactg catggcatca cgcttctcct ctatccggtc aaacacgccc
    12961 accacctcgt aagccccttc aataactttc tccaccacat ttccccggtg tggaacacgc
    13021 aattctccca gagactgacc gcagaccagt ccattggtac aaatggcacg aaaatatccg
    13081 ggtaacatct ggtagctgga tgaaccgtca tgagagttga gcagaataat ttcagggaca
    13141 tgctgaccgg ttatctgtcc ggcccgccgc agacgcagca tgtgttttgt gtattcccgg
    13201 cgacccgggt cacgcacacg tgtctggcag gcgaagaatg gctgaaagcc ttcccgctgc
    13261 aggctttcca gtacggtgat ggtgggagtg tacgcatagc gtttactgcg ggaagtatgc
    13321 cggtcttcac cgaaaatact gggaacatag tgcatcagtt cttcgtgtgt cagcggacgg
    13381 tcacggcgta tctggtttgc agcataacca aaacgactgg ctagtcgcat aatttgctcc
    13441 ttatcggtgg ttaagattta ctggtgtaat aaatgaaaaa gccacgtctc ccggagaaga
    13501 cgcggcctga tggacgaaat taatgaggtt tattgtctga gaagaccgtc actggcgaac
    13561 cttccggcgt cagtctctgc gttgcggata acccgacgga aatgagttcc ctggtcatcg
    13621 tcgataaaca cattgcggta agctgttgtg atgacaacag cctgctcgcg tgagaaaggg
    13681 acttcgggca ataaacgtga catacaacca gacctgtatt tggttccctg aaaatataag
    13741 tccctgtcat catggcagag ccgtatttac ttttgatatc aataaattaa taacgcgaag
    13801 atgaaccgca taacttacgc ctttaatttg ttttgtttac gtagtccctg accaggtacg
    13861 cgaattcgtg aacgccagtt tcaccaaacc aaatcaggta accagggtcg atgttgtaat
    13921 agtaaccaac gactttatgc gtttttgggt tatagagtgt tacgatatag tcccagccgc
    13981 aggcgtgggc tttacaaccg ccaccttcta cccaggtcgt tccgttaatg tttacaggat
    14041 acgcaccacc atcaggacca tccatatagc cgtcttcccc aaacattccg tttaacccaa
    14101 cagggtttaa tgctttgtcg atagcagctc tgtaggtagc gtcaccttcg tataactcgt
    14161 acgttgttgg acatgcacca ggctggtcag cccgggcttt tgagtcgcat ttaagtgtct
    14221 gatttacagt ggcggcgttt gtgtcaggac tt
//
LOCUS       DAJTYF010000101        13990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTYF010000101 DAJTYF010000000
VERSION     DAJTYF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.101"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..170
                     /locus_tag="OO590_003107"
     CDS             <1..170
                     /locus_tag="OO590_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6638390.1"
                     /translation="RINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPK
                     RYRKKLTEFNQ"
     gene            275..1627
                     /gene="celF"
                     /locus_tag="OO590_003108"
                     /gene_synonym="chbF"
     CDS             275..1627
                     /gene="celF"
                     /locus_tag="OO590_003108"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6638391.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1640..2398
                     /gene="chbG"
                     /locus_tag="OO590_003109"
     CDS             1640..2398
                     /gene="chbG"
                     /locus_tag="OO590_003109"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6638392.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2445..4706)
                     /gene="katE"
                     /locus_tag="OO590_003110"
     CDS             complement(2445..4706)
                     /gene="katE"
                     /locus_tag="OO590_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6638393.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIREHVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4889..5152
                     /gene="cedA"
                     /locus_tag="OO590_003111"
     CDS             4889..5152
                     /gene="cedA"
                     /locus_tag="OO590_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6638394.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5429..6245
                     /locus_tag="OO590_003112"
                     /pseudo
     CDS             5429..6245
                     /locus_tag="OO590_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6249..7639)
                     /gene="tcyP"
                     /locus_tag="OO590_003113"
                     /pseudo
     CDS             complement(6249..7639)
                     /gene="tcyP"
                     /locus_tag="OO590_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(7772..8362)
                     /locus_tag="OO590_003114"
     CDS             complement(7772..8362)
                     /locus_tag="OO590_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6638395.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(8525..9192)
                     /gene="hxpB"
                     /locus_tag="OO590_003115"
                     /pseudo
     CDS             complement(8525..9192)
                     /gene="hxpB"
                     /locus_tag="OO590_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            9339..9875
                     /locus_tag="OO590_003116"
     CDS             9339..9875
                     /locus_tag="OO590_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6638396.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(9916..10776)
                     /locus_tag="OO590_003117"
     CDS             complement(9916..10776)
                     /locus_tag="OO590_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6638397.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPSFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(10882..11172)
                     /gene="ghoS"
                     /locus_tag="OO590_003118"
     CDS             complement(10882..11172)
                     /gene="ghoS"
                     /locus_tag="OO590_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6638398.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(11273..12202)
                     /gene="pfkB"
                     /locus_tag="OO590_003119"
     CDS             complement(11273..12202)
                     /gene="pfkB"
                     /locus_tag="OO590_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6638399.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12488..13246
                     /locus_tag="OO590_003120"
     CDS             12488..13246
                     /locus_tag="OO590_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6638400.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(13299..13406)
                     /locus_tag="OO590_003121"
     CDS             complement(13299..13406)
                     /locus_tag="OO590_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638401.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(13578..>13990)
                     /locus_tag="OO590_003122"
     CDS             complement(13578..>13990)
                     /locus_tag="OO590_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638402.1"
                     /translation="PGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLLKGAT
                     AQGPPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIA
                     IHHKHYKTVETYYAAINVISQSLSFSADEIKTYL"
BASE COUNT         3608 a         3213 c         3456 g         3713 t
ORIGIN      
        1 tccgtattaa ttttgccaaa aaacaactgg aaatgaccaa ctattccgtg acggatattg
       61 cgtttgaggc cggttatagt agcccgagtt tgtttattaa aacgtttaag aaattaacgt
      121 cctttacgcc taagagatat cgtaagaaat tgactgaatt taatcagtaa gtcgttatac
      181 ctgacaattc acatatctgt cctgttgctg gattatttat gtccgggggg gcagatatgc
      241 cagatatcag tattctgtac tgaagggaga aattatgagc cagaaattaa aagtcgtcac
      301 tattggtggc gggagcagct ataccccgga gttactggaa ggatttatta agcgttatca
      361 cgaattgccg gtcagcgaat tatggctggt ggatgtcgag ggtggcaaag ctaaactgga
      421 tattattttc gatctctgcc aacggatgat tgataacgct ggcgtcccga tgaagcttta
      481 taaaacgctg gattgccgcg aagcattgaa agatgctgat ttcgttacta cccaactgcg
      541 cgttggccaa ttaccggcgc gtgaactgga tgaacgtatt ccattaagtc atggttatct
      601 tggtcaggaa accaacggcg cgggcggttt gtttaaaggt ctgcgtacca ttccggtgat
      661 ttttgacatc gtaaaagatg tcgaagaact ttgtccgaat gcatgggtga ttaacttcac
      721 taacccggcg ggaatggtta ctgaagccgt ttatcgtcat accggattta aacgctttat
      781 cggcgtgtgt aatattccga tcggcatgaa gatgtttatt cacgatgttc tgatgctgaa
      841 agacagcgat gatttatcta tcgatttgtt tggcctcaac catatggtgt tcattaagaa
      901 tgtgctggta aatggcaagt cgcgctttgc cgaattgctt gatggtgtgg catccgggca
      961 gttaaaagca tctggcgtta aaaatatttt cgatctgcca tttagcgaag gcttaattcg
     1021 ttctctgaat ctgttgccgt gttcttattt gctttattac ttcaagcaaa aagagatgct
     1081 ggctattgaa atgggcgaat actacaaagg cggcgcacga gcgcaggtag tacagaaagt
     1141 cgagaaacaa ctttttgagc tgtataaaaa cccggagttg aaagttaagc cgaaagaact
     1201 ggaacagcgc ggtggggctt attactctga tgcagcgtgc gaagtgatca acgctatcta
     1261 caacgacaag caagctgaac attacgttaa tatcccgcat catgggcata ttgataatat
     1321 tccggcagac tgggcggtag aaatgacctg taagctgggg cgcgatggcg cgacgccaca
     1381 tccgcgcatt acgcatttcg atgataaagt gatggggctg attcacacca ttaaaggctt
     1441 cgagattgct gccagcaacg ccgcacttag cggagaattt aacgatgtgt tactggcgct
     1501 aaaccttagt ccgttggtgc attccgatcg cgatgctgag ctgctggcac gcgagatgat
     1561 tctggcgcac gagaaatggc tgccaaattt tgccgactgc atcgcagagc ttaaaaaagc
     1621 acattaaccg aggctgatta tggaacgctt actgattgtt aatgccgatg attttggctt
     1681 aagcaaaggt cagaactacg gcattatcga ggcctgtcgc aatgggattg tcacatcgac
     1741 gacggcgctg gtgaatgggc aggctattga ccatgcggtg cagttgagtc gtgatgaacc
     1801 gagtctggcc atagggatgc actttgtcct tactatgggc aagccactga cagctatgcc
     1861 ggggttaacc cgcgatggtg tgctgggaaa atggatctgg cagttggcag aagaagatgc
     1921 tttaccgctg gaagaaatta ctcaggagct tgccagtcag tatttgcgtt tcattgagct
     1981 atttggacgc aaacctacgc atcttgatag ccatcatcat gtgcatatgt tcccgcagat
     2041 tttcccgatt gttgccaggt ttgcggctga agaggggatt gcgttgcgca tcgaccgtca
     2101 gccgctatct aacgctggtg atttaccggc taatctgcgc agttcgcagg gattcagtag
     2161 cgcgttttat ggtgaagaga ttagcgaggc actgttcctg caagtgctgg atgacgcaag
     2221 ccatcggggt gatccttcgc tggaggtgat gtgtcatccg gcgtttatcg ataatactat
     2281 ccgtcagagt gcttactgtt tcccacggtt aacggagctg gaagtgctga catcagcgtc
     2341 gttgaaatat gcaattgctg agcgtggtta tcggttgggt agttatcttg atgtgtaagt
     2401 aaggtggtaa ggtcactccc caggggacgt gaccttatcc atatttacgc cggaattttg
     2461 tcaatcttag gaatgcgtga ccacacgcgg tgttctgcca tcagcgttaa cagttcatcc
     2521 ataaaactac cgtcggtgct gtcagcttcc acaatccctt cttcaccctg gtcagctacc
     2581 ttgattgttg ctttaaactt gcgcgcgtct cccgccagcg caatcggttt aaggtgtttg
     2641 taggcttcca tcagatagta gttggcatcg ccgttgtcag cgatatccgc gatattgccg
     2701 caagggacaa tgaccgcatc gaccgtcagc gaaggtgcac cggcaaaggt agcggctata
     2761 ggcaacaccg taccgtcatc cgcagtcact tcacccattc gggagtagag cagtttggca
     2821 tgaacgcctt tggccttcag ggccttgaga atggcaagaa ggtctgccga tctcacttca
     2881 tcattaagta aaatcgctac cacgcgacct ttcacatcac cgtcaggaat ggcgtacaaa
     2941 cttaaggatg gatccttttt cagaccgttg acgtccggag gtggggtgat attcagctgg
     3001 tcgtcagtca gttcgatacc gagatttttc gccaccgcct gggccagagt gagatcaata
     3061 tgcgccagct ggtcaacaac gtgctcacga atatacggac gaacgacttt gcttaactca
     3121 aaactgaaac catcgacaat atggcgctgc tcgaatggcg tctgacttag ccagaacaga
     3181 cgcggatggg aataatattc gccaaacgat gggctgcgct cgcgaacttt attgccttcc
     3241 acgcgctctt ggtatgattc aaaaccgccg cgtttcggcc ccggtggtgt ttcgcgcggc
     3301 cagttatcgt tgatcgagtt cggttcataa ttcgccgggt tagtgtcgat ccccatacga
     3361 tgcatgccgt cacgctggaa attatggtaa gggcaggtcg ggcggttaat cggaatctca
     3421 tggaaattcg gcccaccaag acgactgatt tgtgtatcgg tataggagaa caaacgtccc
     3481 tgcaacaggg gatcgttggt gaagtccaga ccaggaacaa tatgacctgg atggaatgct
     3541 gcttgttcgt tttcggcaaa gaagttatcc ggattgcgat tgagcaccat tttgccgaca
     3601 cgctgaacgg gcaccagttc ttccgggatc agtttggttg gatcgagaag atcgaagtcg
     3661 aacttgaatt catcttcttc aggaatcaac tggaagccca gttcgtattc cggaaaatcg
     3721 cctgcttcaa tggcttccca caactcgcgg cggtggaagt ccgggtcacg tccggtgagt
     3781 ttttgtgctt catcccaaac gagtgaggct ttacctgcca gtggtttcca gtggaaacgt
     3841 acaaacgttg ccttcccttc ggcattaatc aggcggaagg tgtgaatacc gaagccttcc
     3901 atggtgcggt aactgcgggg gatgccgcga tccgacatcg cccacatcac gttgtgcaga
     3961 gtttcaggtt gcagagaaac ataatcccag aaagtatcgt gggcactttg cccttgtgga
     4021 attgcccagt gcggttctgg ttttaccgca tgaacaaaat cggggaattt atgcgcatcc
     4081 tggataaaga agattggcgt gttattgcca acgaggtcaa aaataccctc ttcagtatag
     4141 aacttggtgg caaaaccacg gatatcacgc acggtatcgg cagagccagc gccaccctga
     4201 acggtagaga acctaacaaa tactggggtg attttgttcg gatctgagag gaaatccgct
     4261 ttggtgatat cgcttaagct tttatatggc tggaaataac cgtgagctgc tgatccgcgt
     4321 gcatgaacaa tacgttccgg gatgcgctca tggtcaaagt gggtgatttt ctcgcgcaga
     4381 ataaaatctt ccagcagcgt tggaccacgg ttaccggcac gcagtgagtt ttgatcatcg
     4441 gcgatgcgca cgccctgatt agtggtcagc gcataatttt cactgccttt gcgtacgtct
     4501 tccagagaat taagtttttc gttacgtgta tcaggggctt tcaggctccc aggggcggta
     4561 ggttgtgcac ctggcggtgt tggttcagcc gctggacgat gagatccgtc ctcaggtgcc
     4621 agtgagtcca tccccggttt cgcttcgctg gaatcgtgta gtggtgactg gtgctgatgt
     4681 gggttctttt cgttatgttg cgacattgaa ctcgtctcct taatttatta ctgaaagggc
     4741 cgctgttttg gtcaaaaaac tctctaacta tagaccacca gccagattcc gcttcggaga
     4801 ctattctgga aacggtgtta tgaaatacac gcaaagcggg acgtacaggg gctaaatcgg
     4861 ctaaactaca gtttttctaa tttttaatat gcgtttagtg aagccagtaa tgaagaaacc
     4921 gctccgccag caaaaccgcc agattattag ctatgtccca cgcacggaac ccgcgccgcc
     4981 agaacatgcg ataaagatgg attcgtttcg tgatgtctgg atgctgcgtg gcaaatatgt
     5041 tgcgtttgta ctgatgggag agtcatttct gcgctcaccg gcgtttactg tgcctgaatc
     5101 tgcccaacgt tgggcaaatc agatccgcca ggaaggggaa gtgactgagt aaggattaca
     5161 tacctcctgg ttttgccggg agttacagga cctgaaattc tgcatttcgt tgctacattc
     5221 attcaaacca cctgtaacaa aaagctgcct ttgacgagct attgctataa ataagtgatc
     5281 ttttttcttt aattgtttat ttattttaaa atggttaagt tttgctaatc atgatgagtt
     5341 atttttgata gttcatcctg tggcgtcgct cttaaactta tattgtaaat ggcttagatt
     5401 taaataagat aataattttg ttaacaatat gaggcgggag atgttttcta caaacttaat
     5461 acagagtaat tatggtgatt taaatattaa aagcctggct ttcgactctt ttaaagaaag
     5521 gcttgagtct accatggcgg cactcacttt ctttatatca acgggtcaat gtgactgcga
     5581 tgaaatagca gagtcgaact tcaatcgtat gattgcctat atgtctaaca ttaactatga
     5641 tgcctcaaag ctaggagcgc cagcacttag ttttgatacg tatctgcagg acaatgttaa
     5701 atatagagtg ataattaata atctttatgg cagtgagttt cggataagag atattaatga
     5761 ggattttgtt gggatggatt ttacctgtga attccgaccc gaaaaaatga cgaatttgat
     5821 caatataaaa aataggttgg ttattcatta tttaaggact atatactatg aaaaaaatta
     5881 tatacatcct gccgggagca tttttgctgc gattcaaaaa aaatgagagt ctcctaaagt
     5941 ttccttcgat aatatcaatg cttaatatca atcttctttt taatccacta aatcttccgg
     6001 gaatgggaag tggtgtgctt gaagatatta tgagtattcc agatagttca cttagaaaac
     6061 gtttcgggta tgaggtttta agcttctcac tacaggcaca ttcattgagc caggaatgta
     6121 ttgataagtt ggacattttt ttcgctgacg atctgtttaa atatgagtca gtatgcatcg
     6181 ccgcgatgga acatttaaaa agtaaagcaa cggcccctat acaaaacgga ccgttgccag
     6241 cataagaatt aacggtgtgc cagttcggcg tcgtcttcgc tatccagaat ggctttatcg
     6301 gtttgcttca gccactggct ggtcagcgtg ccagctgtca tcgagccact aacgtttagc
     6361 gccgtacggc ccatgtcgat aagcggttca acggagatta acagcgccac cagggttact
     6421 ggcaggccca tcgcaggcag cacaatcagt gcggcgaaag ttgcaccacc accgacaccg
     6481 gcaacgcctg cggaactaac ggtaacaata ccgaccagcg tcgcaatcca catcgggtcc
     6541 agcgggttaa tgccaaccgt aggcgcaacc atcaccgcca gcattgccgg atacaaaccg
     6601 gcgcagccgt tctgaccaat ggttgcaccg aaagaggcgg cgaaactggc gatggattca
     6661 ggaacgccca gacgacgcgt ttgtgcttcc acattcagtg ggatagacgc agcactggaa
     6721 cggctggtaa aggcaaacgt cagcacaggc cataccttac ggaagtactt cagcggactc
     6781 acgccattaa tgcccagcag aatgccatgc acagcaaaca taatcagcag accgaggtag
     6841 gacgcgacaa cgaaacttcc cagtttgatg atgtcttgca agttagaacc tgcaaccact
     6901 ttggtcatta gagccagaac gccgtaaggg gtcaactgca tgacaaggcg aaccagtttc
     6961 atcacccagc tttgtagggt atcgatagcg gttaagacgc gttcaccttt cggcgcatca
     7021 tccttcagca gtttcaatgc agctacgccg aggaatgcgg caaaaattac cacgctgata
     7081 attgacgtcg gattggctcc ggtaagatcg gcaaacgggt ttttcgggat aaaggacaag
     7141 accagctgcg gaacgctcag atcagagact ttaccaacat agttactttc aatggcgttc
     7201 agacgtgcag tttctgcacc accctgaacc agaccttcag ccgtcaaacc aaacaggtgg
     7261 tgaccagcac accgaccagc gccgcaatca gcgtggtaaa caaaagcgta ccgatggtca
     7321 gaaaactgat tttgcctaac tgagatgcgt tatgcagacg ggcaaccgcg ctcagaatag
     7381 aggcgaagac taacggcata acgatcattt gcagcagttg aacattgccg ttaccaacga
     7441 tgttaaacca ctgtacagaa tctttaagta cctggctgtc agaaccataa atggtatgca
     7501 gggcaaggcc aaaaaccaca cccatcacca gacccaccag cacttttttc gccagactcc
     7561 actgtttatg gcgggtctga gccagcgcaa acagcagtac aacgaacacc acgatgttcg
     7621 caattaatgg aaagttcatc cccgttctcc tgatcttatt atttctatca ctgcgtagca
     7681 gtcatagtgt tggcgcaaga ttaacagaac tgtgagctgg ctcttatatt caaatggaat
     7741 tgcttattcc agaaaggtga tttttgcctt tttagtattc aacctgatgc ttaataagcc
     7801 gatgaaactg gatctgcaag gtattgatca tttgcgatga ccaacgaaca gcgctgttct
     7861 cgggtaatga atggggcatc cataccgacc agacaaataa tgccatgcag ataaaacgtt
     7921 ccagttggtt gcccttttgg ggaaccagga taggcaagcg gaaacgccag cggcatggcc
     7981 agagcagggg aacaccggcg ggtgtcagca tatcggcaag tatgtggctc aaataaccca
     8041 gcaccattcc ttgtagcgca tcagccggaa tgaaccagcc ttccggaacc ttaaggtaaa
     8101 aggttgccag cagcgcaaat accgccagca gactgtgggt aaaaccacga tgcccaaaag
     8161 cgcgggcgat cggttttgat atccatttta atcgctgccc aagaaacgac tttgggtgat
     8221 cgatgtccgg taacaaacac gtcaggattg cggaagggac aatatgccac cagtcaccct
     8281 gtgccagcac gggcgtcagc tcggcatttt tggcaaatac cgcacaagca atagaaaaga
     8341 gaaggtgacc ttccgccgtc atgataaaac tcactaaact gtcgatacgt acagtatagg
     8401 atttttatac agtgcacgga agtggtgacg gtgtaactct ttctcttaaa ttgacgatta
     8461 atccgtcagt tgaatgcaaa ttcagataaa gaaccagggg cagcgtgatc actgcccctg
     8521 gtcattaacc gagaaggtct tttgcggtga gttctgtcag cgatgagagt ttgacgtttg
     8581 ctaatacaaa acgtggatca ttttgcgctt ctggcgcagg aacgacgatg gaacgcatgc
     8641 aggctgcttt agaagcgatc atgccattta ccgagtcttc cagcgccacg caggtcagtg
     8701 ggtcaacgcc cagttttgct gcgcagtcga gatatacttg cggatgcggc ttgctgtaag
     8761 gcagtttttc agccgaggcg agggcatcga aactgtcgcg taagtcaaac atggtcaaca
     8821 ctttttccag catatgtagt ggtgacgcgg aggccagtcc caccaataaa ccttgttctt
     8881 tgcataacgc aacagcttcg cgcacgcctg gtaataatgg acgtgtctct tcaaccagtg
     8941 aaatggcacg ggcaataacc cgttctacta cttcctgacg gcttggccca ttccacggtt
     9001 gccgggcgta caaagatcga ccaccatatc gatgcgtaaa cctaaggtgt ccggcagctc
     9061 gttacgacgg gagatatcta cccccaggct tgccatcaca tccagttcgg ctcgatccca
     9121 taaaggttct gagtcgataa gtaatccatc catatcaaaa attgcagcaa gaatctgacg
     9181 cggggttgac attacaacct ctcctttaga gcattatgtg taaggtatta agccgtgctt
     9241 atgattaagc gcctatcata tcgcctttaa agaatgggcg aaaacagata tctacaggta
     9301 aactaagtga aacgacgcgt cttaatcaag gagaactcat gacgtatcaa caagctggac
     9361 gcattgctgt tttgaaaagg attttgggtt gggtgatttt tatacctgca ttgatctcta
     9421 cattgatttc tttgctgaaa tttatgaata cccggcagga aaaccaggaa ggcattaatg
     9481 cggtcatgct cgactttgct catgtcatga tcgatatgat gcaggcgaat acgccttttt
     9541 tgaatctgtt ctggtataac tccccgaccc ctaattttaa tggtggtgtg aacgttatgt
     9601 tctgggtgat tttcatcctg atttttgtcg gactggcgtt gcaggattcc ggtgcccgga
     9661 tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga tcaactcatt ctggaaaaag
     9721 ccaaagggga agaggggcta acgcgtgaac aaattgagtc ccgtattgtt gttccgcacc
     9781 atactatttt cctgcagttt ttctcactat atatcctgcc ggttatttgt attgctgcag
     9841 gttatgtgtt cttttctctg cttgggttta tttaatcaaa aggccgcacg gagcggccat
     9901 tatcctcaac ccatatcatg ctgctaataa tctatccaat gactgctgag caataaccaa
     9961 atgctgaccg ccaaataatc ttgcacgatt taacagcgtg tagagttggt aaaccggttg
    10021 acgttcaagg aaatcggcag gtagcgggga tactgactga tagccgtcat agatttgtgg
    10081 cggctgttca gtatgcagcg gtaacatcgc caggtcgcac tctcggtcac cccagtagca
    10141 ggcagggtcg aaaatgtacg ggccatccgg acccagcgca cagttgccgg accataaatc
    10201 gccgtgcaac agagaaggct gcggttgatg cgaggccaga cgctgctgaa tatgctcgac
    10261 gatggcgtcg atattgccga aagcgatccc tttctctgct gccagttcca actgccagcc
    10321 aatccgttgt tcagcaaaaa acgttgacca gcgacgttgc caggtgttgg gctgtggagt
    10381 tgtggagagc gagttatcaa aatcgaggcc aaattgtggt tggtcactcc attgatgtaa
    10441 acgcgcaatt tgctgaccaa gaataaatgc gctatgcgca tccagcggac gaggtgggag
    10501 ataatccatc accagaaaac tgtagtcacg gtcagcgcct actgcccaaa ccttaggcac
    10561 ggtgacggtt ttactacgcg acagtaactc cagttggtcg gcttcggcgg taaaactggg
    10621 aagcagttcc ctttcatcac atttgacgaa aaagtcatgt cctgcatagc gcaaatgcca
    10681 tgcggcatgg acttctccgc caggcagttc attacgcagt tcgatttcgc cttcacctaa
    10741 ctgctcgctc aaaagacgac tgattgcctg ccacatgatg tctctcccat gttgtctgcc
    10801 agatcataag gttagcgcaa gaatgcggtg taaaaacacg agctaacgca caccacagac
    10861 tgattattgc ccaaaaacca tttattttct gttgctattc cattcctccc aggtcgtgat
    10921 ggtgatttca ggatctttgc cggttgcact ttgggtaagc cccgaaacca gttctctaat
    10981 ttcttcttca ctttgggtac tgataagtcc aaaagtgtta gtccccagct catggatatt
    11041 tccctcatca tccgtcatgg tgagcaaaaa accgtcgcga gtcaggtaat tattcaactc
    11101 gttaatttca gtcaatgtat cctcatgcaa catgacggtt atgacataac ggacaagatc
    11161 gccactggcc atagttcacc cctttgttat cacatgactt ttgagcatag tcggagaaac
    11221 gcgttgccga caggttggtg gtgattcccc caatgctggg ggaatgtttt tgttagcggg
    11281 aaaggtaagc gtaaattttt tgcgtatcgt catgggagca cagacgtgtt ccctgattga
    11341 gtgtggctgc actccccgca gctacgccaa aacggaccat ctcttcaaga gaggcatttt
    11401 ctgccagttt cagtgtcatc gcgccgacca tgctgtcacc agcgccaacg gtactctggc
    11461 ttttcaccgg tggtggcacc acctgaatgc agttttcact atcaacaccc agcgctcctt
    11521 gtggaccaag ggaaacgaca acccgtttgg ccttgccgct attaacgatt tcctgcgcgg
    11581 ctttgcggac atcgtctggc tgggtgagtt cgcgactcac cagcgcactg agttcttttt
    11641 ggttaggctt aaccaactcg atgttaccaa tcgccagtgc tgcacttaac gcttcgccag
    11701 aactgtcgat gatgcagcgg atcccttggt tttgcgcagc ggaaatcagt tgggttaatt
    11761 tttccagctt cacacctggc ggcaggcttc cgcttatgac caggatggcc ccggattcaa
    11821 tttccagaac ttgctcttca agctggcgaa actcatcttc atttaatgcc gcgcctggca
    11881 taacaaaacg atactgttca ccgcttgctt ccacatgtac gtgtaaattc tgccgggtcc
    11941 agtctttggc ttctacagta gcgacgggga cattttcatc cgccaacagt gaaaccaggt
    12001 gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc actgcctcca agatgggcaa
    12061 tggcgcgggc gacgttgatg ccgccgcccc cgggttcgaa caccggtgcg gtacagcgca
    12121 gttttccttc gggataaatt tgcggggtaa ttgttgcgct atcgagagag ggcgcaagtg
    12181 tcaacgtata gatacgtacc atcatttcct cctataggct gatttcagtc tggcaccgaa
    12241 tcagcggaaa gtgaagaaat taacaatatg atttattaga gctttattta aaattttgca
    12301 gataaatata tataaataaa aatctgtttt cgccacatgc ttattcaaaa aatgaaataa
    12361 gaatacattg ctaagttatt cgcgcctttt tataattcgt tacctttcca gggacgctaa
    12421 tctttgatcc ttaagaaagt ttccgggacc gtgatggtct cttttttgtt gtacggactc
    12481 ttgataaatg aagcttttga agacagttcc tgccatcgtt atgttggcgg ggggcatgtt
    12541 tgcgtcactg aatgcagctg ccgatgattc cgtttttact gtcatggacg atcctgccag
    12601 cgctaaaaaa cctttcgaag gtaatctgaa cgcaggatat cttgcacaat caggcaatac
    12661 gaaaagctcc tcacttacgg ctgacaccac catgacctgg tatggacaaa ccactgcctg
    12721 gtcgctgtgg gggaacgcca gtaatacttc ttctaatgat gagcgttctt cagaaaaata
    12781 tgcggcgggc gggcgtagcc gctttaattt aactgattat gactatttat ttggtcaggc
    12841 aagctggctg acagaccgtt ataacggcta tcgagagcgc gatgtgttga ccgcgggtta
    12901 tggtcgtcag tttttgaatg gcccggtaca cagtttccgc tttgaattcg gtccaggtgt
    12961 gcgttacgac aaatataccg acaatgccag tgaaacccag ccgctgggct atgcctccgg
    13021 ggcttatgca tggcagttga ctgacaacgc taaatttacg cagggtgttt cggtctttgg
    13081 cgcggaagac acaacgctga actccgaaag cgcattaaat gttgctatta atgaacattt
    13141 tggactgaag gtggcttaca acgtcacatg gaactctgaa cctccagagt ctgcaccgga
    13201 acataccgac cgtcggacaa ccttatcgct gggctacagc atgtaaaatt atctgccgga
    13261 ccgtgttatg cggtctggct gtcttcgttt gtcactgctc agccccaaat cattgctgcc
    13321 caacgtagca acaacatagc gccacagatt gccagtagca ccaccaccat cttccagtgt
    13381 tttttatcaa agcgtttagt cggcatacca gtgtcctgca tatctcttgt gcgtgataac
    13441 ggagtgtaac aaaagggaca ggcatcaagg gattttttgt tattacagcc gtcgggtggc
    13501 gttgtgcaca tggtcactgt ttctgcgttt tatgaaaaaa ataaaattat gtatcgatat
    13561 atatcgtaaa tagctggtta taaatacgtc tttatttcat cagcactaaa actcaggctt
    13621 tggctgatga catttatagc agcataatat gtttctacag ttttataatg cttatgatga
    13681 atggctatat gaacagctga catgttgtca tgatttttag cggccaatag tgtaaataac
    13741 tgatgttgac taaaagaatg tttttttgca aaagcaccca gcgtcgatat ataagataac
    13801 acgacgtctt cattaccctt gctcatggcg atgtataatg cagggggtcc ctgtgctgta
    13861 gctcctttta ataaatccat gatgttagct ttgctcaact tgtatttaaa ggcgatgcca
    13921 ttaattttag agaggaaccg tgtgacacac aaagggtgat tattttccat tgcggcgtat
    13981 aatcctggct
//
LOCUS       DAJTYF010000102        13951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTYF010000102 DAJTYF010000000
VERSION     DAJTYF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.102"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..494)
                     /locus_tag="OO590_003123"
     CDS             complement(<1..494)
                     /locus_tag="OO590_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6638403.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="OO590_003124"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="OO590_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6638404.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="OO590_003125"
     CDS             complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="OO590_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6638405.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2441..3307
                     /gene="folD"
                     /locus_tag="OO590_003126"
     CDS             2441..3307
                     /gene="folD"
                     /locus_tag="OO590_003126"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6638406.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3309..3521
                     /gene="ybcJ"
                     /locus_tag="OO590_003127"
     CDS             3309..3521
                     /gene="ybcJ"
                     /locus_tag="OO590_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6638407.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3629..4150
                     /locus_tag="OO590_003128"
     CDS             3629..4150
                     /locus_tag="OO590_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6638408.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="OO590_003129"
     CDS             complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="OO590_003129"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6638409.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5745..6239
                     /gene="ppiB"
                     /locus_tag="OO590_003130"
     CDS             5745..6239
                     /gene="ppiB"
                     /locus_tag="OO590_003130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6638410.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6242..6964
                     /gene="lpxH"
                     /locus_tag="OO590_003131"
     CDS             6242..6964
                     /gene="lpxH"
                     /locus_tag="OO590_003131"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6638411.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7082..7591
                     /gene="purE"
                     /locus_tag="OO590_003132"
     CDS             7082..7591
                     /gene="purE"
                     /locus_tag="OO590_003132"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6638412.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7588..8655
                     /gene="purK"
                     /locus_tag="OO590_003133"
     CDS             7588..8655
                     /gene="purK"
                     /locus_tag="OO590_003133"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6638413.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8792..9685)
                     /locus_tag="OO590_003134"
     CDS             complement(8792..9685)
                     /locus_tag="OO590_003134"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6638414.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRWVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9682..10329)
                     /locus_tag="OO590_003135"
                     /pseudo
     CDS             complement(9682..10329)
                     /locus_tag="OO590_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10332..11564
                     /gene="allC"
                     /locus_tag="OO590_003136"
     CDS             10332..11564
                     /gene="allC"
                     /locus_tag="OO590_003136"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR6638415.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11575..12360
                     /gene="allE"
                     /locus_tag="OO590_003137"
     CDS             11575..12360
                     /gene="allE"
                     /locus_tag="OO590_003137"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR6638416.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(12588..13733)
                     /gene="glxK"
                     /locus_tag="OO590_003138"
     CDS             complement(12588..13733)
                     /gene="glxK"
                     /locus_tag="OO590_003138"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HCR6638417.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDALFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(13755..>13951)
                     /locus_tag="OO590_003139"
     CDS             complement(13755..>13951)
                     /locus_tag="OO590_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCR6638418.1"
                     /translation="ITFTERNIYRLALPLFVGIFLMALPPVYLQDLPLTLRPLLSNGL
                     LVGILLAVLMDNLIPWERIE"
BASE COUNT         3386 a         3679 c         3495 g         3391 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg ataaacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa agattgcttt
     1261 ggcttcttac aacacatcat tcccttttca tttaataaag cggcatgata caaagtgaac
     1321 gtggtttcga tttacattgc caccataccg cttcagacaa ccggtacatt actttgttat
     1381 aagacgtgat atataattca aaacaacgtg gttttgatta ttcgtatctc ataataaaag
     1441 tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc ggtgccttta taacgtgcag
     1501 aaaaatgaag aacgttggtc ccggggatca agttctggtt ggttgagaag ctattcccat
     1561 caggtttgag aattgcggat gtattatcaa gtatttcaat accgacaccc gttgctgttg
     1621 ttgtatttgt actggatgca atggccaata aattatcatt gattgcatca gcctgaccag
     1681 aaaatgcaac ggcagcatta gcggcaacaa caggatcgca gtcgttcaac tgaatggtga
     1741 atggaattaa tgcagaggta ttaccaacag cattgaaaat atcggtacga tattgaccga
     1801 gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca ggcagcattc accagttcgc
     1861 cttcaaagtg aattgtaccg ccatctacaa cggcagcata actacccgta gcggcgaata
     1921 gtgcggcagc cagcgcagac gaaataaatc ttaatttcat atatattcct tcaatctcat
     1981 ttatcgactc cacatccgta tataaccgat tactttattt aagacactga tagtagtaaa
     2041 attccttttt atcctctaag aatgtcttaa ttgaaaatat gcactctatt ctaaaaaata
     2101 gagagccccg ttagatgaat acttccgcgc aaaatatatt caacacaaat atagacctga
     2161 agcggtaaat taccaggctg aaaattcttt ttatattgtc aggtatttct taaattatct
     2221 taatccttaa acaaggaaat aaatcagttc cagatttaca acgccatcat gtacgaaaaa
     2281 tgaagctttc aatctcagcg acctcagcga cggtgcgcct aaccttcgca agaggtcgct
     2341 tcacgcgata aatctgaaac gaaacctgac agcgcgcccc gcttctgaca aaatagacgc
     2401 atccccttcg acctacgtaa cagatggaat cctctctctg atggcagcaa agattattga
     2461 cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct caaaaagttc aggctcgtat
     2521 tgcagccgga ctgcgggcac caggactggc cgttgtgctg gtgggtagta accctgcatc
     2581 gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa gtcgggttcg tctcccgctc
     2641 ttatgacctc ccggaaacca ccagcgaagc ggagctgctg gagcttatcg atgcgctgaa
     2701 tgccgacaac accatcgatg gcattctggt tcaactgccg ttaccggcgg gtattgataa
     2761 cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg gacggtttcc atccttacaa
     2821 cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc tgcaccccgc gcggtatcgt
     2881 cacgctgctt gagcgttaca acattgatac cttcggcctc aacgccgtgg tgattggcgc
     2941 atcgaatatc gtcggtcgcc caatgagcat ggaactgttg ctcgcaggtt gcaccaccac
     3001 cgtgactcac cgcttcacta aaaatctgcg tcatcacgta gaaaatgccg atctgttgat
     3061 cgttgccgtt ggcaagccag gctttattcc cggtgactgg atcaaagaag gcgcaattgt
     3121 gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt gtgggcgacg tcgtgtttga
     3181 agacgcggct aaacgcgcct catacattac gcctgttccc ggcggcgttg gcccgatgac
     3241 ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt gaatatcatg atccacaggg
     3301 tgagtaacat ggcgacattt tctttaggta aacatccgca cgttgagctg tgcgacttgc
     3361 tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa aatcgcgatt gccgaaggcc
     3421 aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg caaaatcgtc gccggtcaga
     3481 cagtgagttt tgcaggacac agcgtacagg ttgttgcctg atccctttcc cggcctgtat
     3541 caacaggccg gttctctttt cagacttttg ccctctacgc cccgttcatc catgctgata
     3601 gcatcgacat tttgccgaag cgaccatcat gccaaccgtt attacgcacg ccgccgttcc
     3661 cctttgcatt ggtttagggt tgggttcgaa agtcatccct ccgcgtctgt tatttgccgg
     3721 aatcatccta gcgatgctgc ccgatgccga cgtattgtcg tttaaatttg gcgttgctta
     3781 cggcaatgtt tttggtcatc gcgggtttac ccattcgctg gtgtttgcgt ttgttgtccc
     3841 gctcttatgt gtgctgattg gacgtcgatg gttcagggct gggctgattc gctgctggct
     3901 gtttttaacc gtctcgttgc tatcgcacag cttactggat tcggtaacca ctggcggtaa
     3961 gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt ttcgctccct ggcaggtgat
     4021 taaagtcgcg ccgtttgcat tgtctcgtta caccacagcg tacgggcatc aggtgattat
     4081 ttccgaattg atgtgggtgt ggttgccggg gatgctactg atgggaatgt tgtggtggcg
     4141 cagacgataa ccggatgcga aaactcgcat ccggcaattg cgcaattact tacgacgcca
     4201 ggtggtccct tgcgggccat cttccagcac gatccccatc tcgttaagac ggtcacgtgc
     4261 cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc agacgctgtt gaattaacgc
     4321 ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg ctttgcagga acgcttccgg
     4381 ttcttgctcc agcaggccca atacagcgga aagtttacgc aggtgagatg ccattgcatt
     4441 cgccgctgcc atatcttctg ctttcagacg gttcacttca cgcgccatat caaacagcac
     4501 ggaataggct tccggggtgt tgaaatcgtc gtccatcgct tcaataaagc gcgcttcaaa
     4561 cgcttcgcca ccggcaggcg caacggtttt atctgtgccg cgcagcgcag tgtagaggcg
     4621 ctccagcgcc gcacgcgcct gcttcaggtt ctcttcgctg tagttcagct ggctgcgata
     4681 gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg tagtatttca gcacatcgcg
     4741 cacggtaaag aagttaccca gcgatttgga catcttctcg cggtcaacca tcaccattcc
     4801 ggagtgcatc cagtagttca catactgacc atcatgggca caggtggact gcgcgatttc
     4861 gttctcgtgg tgcgggaaca tcaggtctga accgccgccg tggatatcaa agtggttacc
     4921 cagctgcttg cagttcatgg ccgaacattc aatgtgccag ccaggacgac ccgcgcccca
     4981 cggagacggc cagctcggtt cgccctcttt cgacatcttc cacagaacga agtccatcgg
     5041 gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc tgcagctggt cgagatcctg
     5101 acgcgacagc acgccataag ttggatcggt cgggacgtcg aacatcacgt cgccgttgtc
     5161 cgccacataa gcgtgacctt tggcgatcag ttgttcagtg agttcaataa tttctgcgat
     5221 atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg ttcaaagcat caaaatcttt
     5281 gtgcatttcg gcaatcatgc gatccaccag cgccacaaag ctttcgccat tttcattggc
     5341 gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc acatacttca gtttatagcc
     5401 gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca aaggtacgcc cgtgaccgat
     5461 atgacagaga tcgtaaacgg tgattccaca cacgtacatg ccgacttccc cggcgtgaat
     5521 aggcttaaat tcctcttttt ggcgtgtcag agtattgaag atttttagca tcgaagattc
     5581 cgtttagaca tgtgtgggta attgagttgc gtataatacc catatttccc gcgcgaatca
     5641 gcatacattg cgtgatgatc cgatcgtgcg gttatgctat aacaccaccc tatatatgac
     5701 ccgaactggg ttgaagcacc aatcaaacgg aacaggatgc aaaaatggtt actttccaca
     5761 ccaatcacgg cgatattgtc atcaaaactt ttgacaataa agcacctgaa acagttaaaa
     5821 acttcctgga ctactgccgc gaaggttttt acaacaacac cattttccac cgtgttatca
     5881 acggctttat gattcagggc ggcggttttg aacctggcat gaaacaaaaa gccaccaaag
     5941 aaccgatcaa aaacgaagcc aacaacggcc tgaaaaatac ccgtggtacg ctggcaatgg
     6001 cacgtactca ggctccgcac tctgcaactg cacagttctt catcaacgtg gttgataacg
     6061 acttcctgaa cttctctggc gaaagcctgc aaggttgggg ctactgcgtg tttgctgaag
     6121 tggttgacgg catggacgtg gtagacaaaa tcaaaggtgt tgcaaccggt cgtaacggta
     6181 tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag cgtgaccgtt agcgagtaat
     6241 cgtggcgaca ctctttattg cagatcttca tctctgcgtg gaagaaccgg cgatcaccgc
     6301 cggttttctg cgttttttag cgggggaagc ccgcaaggcc gacgcgctgt atattcttgg
     6361 cgatctgttt gaagcatgga ttggcgacga cgatcccaac ccactccatc gccagatggc
     6421 ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt tatttcattc atggcaaccg
     6481 tgattttctg ctcggcaaac gctttgcccg tgaaagcggc atgacattat tgccggaaga
     6541 aaaggtgctc gaactttatg gtcgccgggt gttgattatg catggcgaca cgctgtgcac
     6601 cgatgacgcg ggttatcagg cttttcgcgc caaagtccac aaaccctggc tgcaaatgct
     6661 attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc gcgcgaatgc gcgcgaacag
     6721 caaagaagcc aacagcagta aatcgctggc gatcatggac gttaaccaaa acgctgtggt
     6781 cagtgcgatg gaaaaacatc aggtgcaatg gctaatccac gggcataccc atcgcccggc
     6841 ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc gtggtactgg gtgcctggca
     6901 tacggaaggt tcgatggtga aagtcacggc ggatgacgtt gagctgattc attttccgtt
     6961 ttaaaaaacc cacaactttg ctgatttcac agccacgcaa ccgttttcct tgctctcttt
     7021 ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc accacaggag ttttaagacg
     7081 catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg atggggtcca aaagcgactg
     7141 ggctaccatg cagttcgccg tcgaaatctt cgaaatcctg aatgtcccgc accacgttga
     7201 agtggtttct gctcaccgca cccccgataa actgttcagc ttcgccgaaa gcgccgaaga
     7261 gaacggttat caggtgatta ttgcgggcgc aggcggcgca gcgcacctgc caggcatgat
     7321 tgccgccaaa acgctggtgc cggtgctggg cgtgccagta cagagcgccg cactgagcgg
     7381 tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc attccggtgg gtacgctggc
     7441 gattggtaaa gctggcgcgg caaacgcggc attactggca gcacaaattc tcgcgactca
     7501 cgataaagag ctacaccagc gtctgaatgg ctggcgcaaa gcccagaccg acgaagtgct
     7561 ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt gcgtcctcgg taacgggcag
     7621 ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca ttgctgtctg gccggtcggg
     7681 ctggacgctg aaccggcggc ggtgcctttt caacaaagcg tgattaccgc tgagatcgaa
     7741 cgctggccgg aaaccgcatt aacccgcgag ctggcgcgtc atccggcctt tgtgaaccgc
     7801 gatgtgttcc cgattattgc tgaccgtctg actcagaaac agcttttcga taagctccac
     7861 ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg agtggcctgc ggtgtttgat
     7921 cgtttaggtg aactggcgat tgttaagcgt cgcactggtg gctatgacgg tcgcggtcaa
     7981 tggagtttac gcgccaatga aaccgaacag ttaccggcag agtgttacgg tgaatgtatt
     8041 gtcgagcagg gcattaactt ctctggtgaa gtatcgctgg ttggcgcgcg cggctttgat
     8101 ggcagcaccg tgttttatcc gctgacgcat aacctgcatc aggacggtat tttgcgcacc
     8161 agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc aagccgaaga gatgctgtcg
     8221 gcgattatgc aggagctggg ctatgtgggc gtgatggcga tggagtgttt tgtcaccccg
     8281 caaggtctgc tgatcaacga actggctccg cgtgtgcata acagcggtca ctggacacaa
     8341 aacggtgcca gcatcagcca gtttgagctg catctgcggg cgattaccga tctgccgtta
     8401 ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc tgattggtag cgatgtgaat
     8461 tatgactggc tgaaattgcc gctggtgcat ctgcactggt acgacaaaga agtccgtccg
     8521 gggcgtaaag tggggcatct gaatttgacc gacagcgaca catcgcgtct gactgcgacg
     8581 ctggaagcct taatcccgct gctgccgcca gagtatgcca gcggcgtgat ttgggcgcag
     8641 agtaagttca gttaagcagg tgctcatttg attgccggat gcggcgtaaa cgccttatcc
     8701 ggcttaccgc tccggtaccc attgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
     8761 atggtgcacc gattgccgga tgctctcatc actacagcga aatacaggtc cccgcttcgc
     8821 ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca cgcgggttta ctacggcttc
     8881 tgacataacc actcaccgcc gttaccttcg gccccatcga accatcggct ttggcaaatg
     8941 gcgctaactc atccggtgtg gcatggcgaa tggcacgttg ctgcggcgtt ccccagtttt
     9001 catataccgc atcagcatcg gtgaggatca ccagtccatc ggcattaatc tgctcggcga
     9061 gcaacgcagc ggcgagatct ttatcaatca ccgcttcact ccctgcccca tcttccgtca
     9121 caggcacacc gccgccgcca ctgcaaatca ccacatgccc ctctttgagc aacaactcga
     9181 tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc caccacccag cgcaaatatt
     9241 taccatcacg tttcatctgc cagccgtaag ccgcttccag tgcctcttgt tcttctggct
     9301 gataaaccgg accaataaat ttctctggct gcaaaaacgc cggatcatca ggcgaaacct
     9361 caatgcgcgt cagcaccgtc gtaacaggcg gcatctgcgg ctgtgcgctc aagctctgcg
     9421 ccagcatata gccaatcatc ccctggcttt ccgccaccag cacatccagc ggatacggtt
     9481 ctacctcttt ccacgccaga ttctgtaacg ccagcagccc cacctgcggc ccgttgccgt
     9541 gaacaatcgc cagccgataa gaacgggcca ggcgtgccag cgcgggtaca gcactggcga
     9601 tattgcgata ttgattttcc gccgtcagcg cctcaccgcg ctggagtaag gcgttgcccc
     9661 caagagctac aaccagtgtt ttcatggttt tccttgtaat aattgttggc caagccagaa
     9721 ccccagcagc gtatctgccc ctgacgtatg ccccagcgcc agcagcgaat caatcgcaac
     9781 agcggtacgt ttcgggcaac tcagagcatg gacaaagtgc aggagtggcg aggcgaaata
     9841 tccttgcgcg gcataacgta aataactgac gctcaccgct gtggtaacga gttgaagatt
     9901 gtcggaacag gcaaaaaacg ggcgaccggc gcgcgcatct aaagcgccat aataccaggc
     9961 cgccagcagc attccgctca acgtgtcatc atgacttggc gttaatccgg ggcctttacc
    10021 cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg gcctgaaaac agtgacgaaa
    10081 ctggcgtaat tcagcgggca gcggatcgct tgccgccatc gccagtggcc cgaaaagtcc
    10141 ggtttcctcc gcgcgttgca tccatgcagc tgcaagcggt tgaggatgcg aaggcggcgt
    10201 aatacgcagc aaacaataac gctgtggctg tttaaccgtg aaacgcccga ggcgaatccc
    10261 tttagccaca acctgtggtc gttcattgcc gcataatcca ccgcataacg catcgaattg
    10321 cgtgcggcga aattacacat tcccgtcaag ctatagaaga aacgctgccc tggctttcct
    10381 cttttggcgc tgacccaacg ggtgggatga cccgtttact ttattcgccg gaatggctgg
    10441 aaacccagca gcaatttaaa aaaagaatgg cagcaagtgg gctggaaaca cgtttcgatg
    10501 aagtggggaa tttatacggt cgcctgagtg gcaccgaata tccacaggaa gtggttctga
    10561 gcggttcgca tatcgatacc gtggttaacg gcggtaacct cgacgggcaa ttcggcgcgc
    10621 tggcggcgtg gctggcaatt gactggttga aaacgcaata cggcgcaccg ttacgtacgg
    10681 tcgaagtggt ggcgatggca gaagaagaag gcagccgctt cccgtatgtc ttctggggca
    10741 gtaaaaatat ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt gatgccaaag
    10801 gaaatagttt tgccgatgcg atgaaggctt gcggatttac tctgcctaac gccccactaa
    10861 ctccgcgtca ggatataaaa gcctttgttg aactgcatat tgaacagggc tgtgtgctgg
    10921 aaagtaatgg gcaatcaatt ggcgtggtga atgcaattgt cgggcagcgt cgctatacgg
    10981 tgacgctgaa cggcgagtca aaccatgcag gcaccacgcc gatgggttat cgtcgtgata
    11041 cggtttacgc tttcagtcgc atttgccatc agtcgatcga aaaagcgaaa aagatgggcg
    11101 atccgctggt tctgaccttt ggcaaagtag agccgcgccc gaatacggta aatgtggtgc
    11161 cgggtaaaac cacgttcacc attgattgtc gtcataccga cgctgccgtg ctgcgcgatt
    11221 tcacccaaca gttagaaaac gacatgcggg cgatttgcga tgaaatggac attggtattg
    11281 atatcgattt atggatggac gaagaacccg tgccgatgaa taaggagctg gtcgccaccc
    11341 tgacagaatt gtgtgaaaga gaaaaactga attaccgggt gatgcacagt ggtgccgggc
    11401 acgacgcgca aattttcgcg cctcgcgtac caacctgcat gatttttatc cccagcatca
    11461 acgggatcag ccataacccg gcggaacgca ccaatattac tgaccttgcc gaaggggtca
    11521 aaacgttggc actcatgctt tatcaacttg cctggcagaa ataaggagtc ataaatggga
    11581 tatttaaata acgtcaccgg ttaccgcgaa gatttactgg ctaaccgtgc gattgttaaa
    11641 cacggtaatt tcgcactgtt aaccccggat ggtctggtaa aaaatattat tccgggcttt
    11701 gaaaattgtg acgcgacaat cctctccacg ccaaagctgg gtgcctcttt tgttgattat
    11761 ctggtcacac tgcatcaaaa cggtggcaac caacagggct tcggtggcga aggcattgaa
    11821 acgttcctgt atgtgatctc tggaaatatc actgccaaag ccgaaggcaa aacatttgcc
    11881 ttaagcgaag gtggctatct ttattgccca ccaggctcct taatgacgtt tgttaacgcc
    11941 caggccgaag acagccaaat ctttttatat aagcgccgct atgttccggt agaaggccat
    12001 gcaccgtggc tggtttctgg caatgccagc gaactggaac gcattcacta tgaaggtatg
    12061 gatgatgtga tcctgctgga ttttctgccc aaagagttag gttttgatat gaacatgcat
    12121 atcctctctt ttgcaccagg tgccagccac ggttatatcg aaacacacgt tcaggaacat
    12181 ggtgcctata ttctttccgg tcagggggtt tataacctcg acaataactg gatcccggtg
    12241 aaaaaaggcg attacatctt tatgggcgct tattctttac aggctggtta tggcgtaggg
    12301 cgtggtgaag cgttcagcta tatttactcg aaagattgca accgcgacgt agagatttaa
    12361 gtcataccac tcgcctgatt atttttagct atgttggatt ttgccacggt ttataccgtg
    12421 gctttttttg taacattgcc tgatgcgctt cgcttatcag gcctacatga cctctgcaat
    12481 atatagaatt tgcgcgattt tcaggaagga tatagtactc atgccgcatc cggcatgagt
    12541 actgcgctct tatacgaaat aaatcgcatc cgctttaaag gcaagagtca gtttttaatt
    12601 ccctgaccta ttttaatggc gcaggcaata tttcgcgcgc tgttgaagag attggtttca
    12661 ccgctggcga gcacttcggc taaaggtgcc agacgaggca aaatgctgaa tagcgcgtca
    12721 atgccgtact ggtgcaccac ttccacgcca tcacccaata caccagcaat cccaatcacc
    12781 ggtacattaa actgcttcgc caccgacgca acgcccagcg gcgctttacc gcctgccgtt
    12841 tgcgagtcaa tgcgcccttc cccggtgatc accagtgctg ccccctgcac cgcctgcgca
    12901 agattgaccg cattcaacac aatctcaatg cccggtttaa tatccgcatt gagaaatacc
    12961 gccgccgcaa tccccatacc gcccgcagcg ccgccgcccg ccatctgaca gacattaatt
    13021 tcagtttgct gttgtaaaac acgggcgtaa ttttgcagcc cctgttccag ctcttcaacc
    13081 atctccggcg ttgccccttt ttgcgggcca aataccgccg ccgcgccgcg tgcccctacc
    13141 agcgggttat caacgtcaca tgccacttca atatggctgt tcgccagacg cggatcgcat
    13201 tcctccatct caatgcttgc cacgcgcgct aaattgccgc cgttagccgc cagcacctgg
    13261 ccgtcggcat caaggaaacg cacaccaagc gcctgcgcca tgcccatacc gccgtcgacc
    13321 gtcgcactgc cgccaatgcc gagaataata tggcgaatgc cgttatccag cgcatggcga
    13381 attaactccc ccgtaccaaa actggaggcc agcaacggat tacgcttttc aggcgcgacc
    13441 agcatcaggc cacttgctgc cgccatctca atcaccgctg ttttcccgtc tccggtaagg
    13501 ccataaaaag cattcacttt ttcgcccatc ggcccgcaga cttcaagcgt cacgatgttg
    13561 ccacccgtcg cggcgaccat cgcatccacc gtcccttcgc cgccatccgc tatcggcaaa
    13621 cagatatagt gcgcatcggg aaagagggtc gaaaacccgg ctttaattgc ctgacaacat
    13681 ttttctgcac ttaagctctc cttaaaagag tctggcgcaa tgacaatctt cattatcaat
    13741 cctttttcaa caaattattc gatgcgttcc cacggaataa gattatccat aagaacagcc
    13801 agtaaaattc cgaccagtaa gccgttactg agcagaggac gaagcgttaa tggaaggtct
    13861 tgcaaataca caggcggtaa tgccattaaa aatatgccga caaataacgg caatgcgagt
    13921 cgataaatat tgcgctcggt aaacgttatt t
//
LOCUS       DAJTYF010000103        13872 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTYF010000103 DAJTYF010000000
VERSION     DAJTYF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.103"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            116..1417
                     /gene="rstB"
                     /locus_tag="OO590_003140"
     CDS             116..1417
                     /gene="rstB"
                     /locus_tag="OO590_003140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6638419.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1493..2422
                     /gene="tus"
                     /locus_tag="OO590_003141"
     CDS             1493..2422
                     /gene="tus"
                     /locus_tag="OO590_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6638420.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="OO590_003142"
     CDS             complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="OO590_003142"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6638421.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3965..5611)
                     /gene="fumB"
                     /locus_tag="OO590_003143"
     CDS             complement(3965..5611)
                     /gene="fumB"
                     /locus_tag="OO590_003143"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6638422.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5810..6985
                     /gene="manA"
                     /locus_tag="OO590_003144"
     CDS             5810..6985
                     /gene="manA"
                     /locus_tag="OO590_003144"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6638423.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLRDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANKSPVTVKGHGRLARVYNKL"
     gene            7086..8594
                     /locus_tag="OO590_003145"
     CDS             7086..8594
                     /locus_tag="OO590_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6638424.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="OO590_003146"
     CDS             complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="OO590_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6638425.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSIRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10124..10291)
                     /locus_tag="OO590_003147"
     CDS             complement(10124..10291)
                     /locus_tag="OO590_003147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6638426.1"
                     /translation="MGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKK
                     VQQQLISDITN"
     gene            complement(10307..11497)
                     /gene="uidB"
                     /locus_tag="OO590_003148"
     CDS             complement(10307..11497)
                     /gene="uidB"
                     /locus_tag="OO590_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6638427.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQV"
     gene            complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="OO590_003149"
     CDS             complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="OO590_003149"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6638428.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13693..>13872)
                     /gene="uidR"
                     /locus_tag="OO590_003150"
     CDS             complement(13693..>13872)
                     /gene="uidR"
                     /locus_tag="OO590_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6638429.1"
                     /translation="RDAQRKGEIGPDINTAMTSRLLLDLTYGVLADIEAEDLAREASF
                     AQGLRAMIGGILTAS"
BASE COUNT         3487 a         3624 c         3301 g         3460 t
ORIGIN      
        1 atacatccgt ctgttccgga cattatcttc caaacaaggt cttatccagt tggcggttta
       61 cattgctcca cagtcgtgaa ttaatacgaa tagtattctg ttgagggcga tgccaatgaa
      121 aaaattgttt atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct
      181 ggttgggctg gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt
      241 gatgaacagt tcgctgtatc tgatgcgcag cgaattgcga gagatccccc cacacgactg
      301 gggtaagacg ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact
      361 gagtaaatac catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc
      421 cctggacgat cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt
      481 tggtcctgtt ccttatcttt attacctcca tcaaatgcga ttgctggata tcgccctgat
      541 cgcttttatt gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca
      601 ggatatgtta aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg
      661 tatccacttt gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc
      721 ggacaatatc aacgccttaa ttgccagcaa aaaacagctt attgacggta tcgctcacga
      781 actgcgaaca ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc
      841 cgccgaatcc caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct
      901 gctgacttat gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct
      961 gccgttgtgg ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt
     1021 acggataaaa acgctcatgc aaggccatta tgcggcgttg gatatgcgct taatggagcg
     1081 cgtgctggat aatttgctca ataacgccct gcgctactgc cattcaacgg ttgaaaccag
     1141 cctgctactg tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc
     1201 cccagaaaac cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg
     1261 ctcaaccggc ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg
     1321 cggtacggtt aattgtgaca tcagcgaact gggtggtgct cgcttctcgt ttagctggcc
     1381 gttatggcat aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt
     1441 cacaacgact gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg
     1501 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctac
     1561 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
     1621 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg
     1681 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
     1741 cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
     1801 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
     1861 gttcgagcat atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt
     1921 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca
     1981 cgaccccgcc actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg
     2041 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgtagtg tcgcaccgtg
     2101 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
     2161 acagaacgcg aagttaaaaa tcaaacgtcc agtgaaggtg cagccgattg cccgcgtctg
     2221 gtacaaagga gatcaaaaac aagtccaaca cgcctgccct acaccactga ttgcactgat
     2281 taatcgggat aatggcgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
     2341 caatgtgcag caccgttata aacctcaagc gcagccgctt cgtttgatca ttccacggct
     2401 gcacctgtat gttgcagatt aacgcccggc ttttatactg ccgaccatct gttctggccg
     2461 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
     2521 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
     2581 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
     2641 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
     2701 gcgcaccgat tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt
     2761 accggaagcg ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat
     2821 ggttaatgct tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc
     2881 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
     2941 atcattggcg attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac
     3001 cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
     3061 ggtaatgact gccagttcat ctgctacgcg ccgcgcatac tccggatggg tatttagtcc
     3121 agtacccacc gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat
     3181 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag
     3241 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
     3301 tttttcactc agcgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagtgc
     3361 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
     3421 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acaccgccga gtaattcact
     3481 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
     3541 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
     3601 ccgaatggcg ctcgctttct cttcagacaa caagtctaaa tcttcattaa cttttgccgc
     3661 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
     3721 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
     3781 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctga
     3841 ttaatttttt ctttctgttt tgctttcgtt aagcaacttt tagctcactc attatttact
     3901 atttgataac aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
     3961 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
     4021 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
     4081 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
     4141 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
     4201 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
     4261 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
     4321 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtattgcg
     4381 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccaa
     4441 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
     4501 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
     4561 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
     4621 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
     4681 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcggat gtcgtgagcg aagtatttac
     4741 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagtaattct ttttccagtt
     4801 ccacatcgcg gaacgcctga ccgtgctcat tcccttccgt tggcagttca tcatagtatt
     4861 tcgcggaagc cagtttcacc gttttaaggt tcgtttctgc agaagttcca ccaataacga
     4921 acgcaatatg atacggagga caggccgcgg tacccagcgt gcgcatcttc tcaaccaggt
     4981 aatttttcag tttccccggc gtcagtaacg ctttggtttc ctgatagaga tacgtcttgt
     5041 ttgccgaacc accacctttt gcgatacaga ggaatttgta ttcgtcgcca tcaacggcat
     5101 aaagatcgat ctgcgctggc agattggtgc cggtattcac ttctttatac atatccagcg
     5161 gcgcgttttg cgagtagcgc agattatctt cgatataagt gttatagaca ccgcgcgcca
     5221 gcgccgcttc atcaccacca ccggtccata cacgctgccc ttttttacca acaataatcg
     5281 cggtgccggt atcctgacag gttggcagaa cgcctttcgc cgcgatgtcg gagttacgca
     5341 ggaattgcag cgccacatat ttatcatttt cgctggcctc cgggtcacgc agaatgtcgg
     5401 ccacctgttg ttggtgagcc ggacgcagca taaacgacgc atcatgaaac gcctgacgcg
     5461 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
     5521 cgctaacgtg ttcactggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
     5581 gagactgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
     5641 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacagtt
     5701 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
     5761 tcttgcttta atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact
     5821 cattaactca gtgcaaaact atgcctgggg tagcaaaacg gcgttgactg aactttatgg
     5881 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
     5941 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag
     6001 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
     6061 gttcaaagta ttatgcgcag cacagccact ctccattcag gttcatccaa acaaacacaa
     6121 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
     6181 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
     6241 gatgaacgcg tttcgtgaat tttccgagat tgtctcccta ctccagccgg tcgccggtgc
     6301 acatccggcg attgctcact ttttacaaca gccacatgcc gaacgtttaa gcgaactgtt
     6361 cgccaacctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
     6421 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
     6481 cccggaagac agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
     6541 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
     6601 agtgatggcg aactccgata acgtgctgcg tgcaggtctg acgcctaaat acattgatat
     6661 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
     6721 gccggtgaaa caaggtgcag aactgggctt cccgattcct gtggatgatt ttgccttctc
     6781 gctgcatgac cttagggata aagaaaccac catcagccag cagagtgccg ccattttgtt
     6841 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
     6901 tgaatcagcg tttattgccg ccaacaaatc accggtgact gtcaaaggcc acggccgttt
     6961 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
     7021 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
     7081 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
     7141 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
     7201 aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
     7261 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgctggga
     7321 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
     7381 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
     7441 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
     7501 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
     7561 tcaatccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
     7621 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
     7681 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
     7741 ccgacggttc cagcacgctg ggaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
     7801 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
     7861 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
     7921 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
     7981 tcggccagat tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc
     8041 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
     8101 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg
     8161 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
     8221 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
     8281 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag ttgatgactc
     8341 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
     8401 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
     8461 tcaccaccag cctgcaatat gctaacggtc agataacgtt aaatgggcag aaaatgtcgt
     8521 tagaagattt tgttggcatg tttgcgatgc cagctcttaa cgttccggct gtaccggcaa
     8581 ttccgcagca gtaattcacg gccaaaacgc cggagaattt tcactccggc gttttcatct
     8641 gaaaacaaac cagagattgc ctgatgcgct acgcttatca ggcctacctt ttcctgcaac
     8701 actttgaatt tatgagtttt tgtaggctgg ataaggcgtt tacgccgcat ccggcataaa
     8761 aaacgcgcac tttgtcaaca atctgaaacg ccggaggttt ttctctccgg cgttatttct
     8821 tacttcagca taaagtcata ctgaatactc attttcacat cccagtaatt caggcgatcg
     8881 cccagcgtac tgccatcgcg cttttcccgc cctttatcgg cagcaagaaa gaaattcatc
     8941 ccttgtaacg gcccttcgct gacccgatag ttcataccac ctaagatacc cgcgccttca
     9001 atccgcacat ctcgtttact ggattcatag ccgcgagtca caatcggtgc aaatgtcagc
     9061 gtaaattgcg gagttacacg gtagttgaca cctaattgcc aggactgcat tccttcacgg
     9121 ttacgatcaa gacttaatga aaagggatag ccaatgtcgg tatccaccac ggcaccattc
     9181 attgcatggc gcaaataacc accactgcca aatacgcttc cccacggcgc attccaggtc
     9241 aattgtccac taaccaacgc cgtttcattg ggctggctgg tattacgact taaaccttcg
     9301 agctgcgcgt aaaagccgag caactcccct ttcaacgtat aattcgggtc gagcggtatg
     9361 cttttctcga gaatactttg tatctgattg gaaacatctt tctgtaccga tgcccccaga
     9421 gaatagcgac tggtatcatc atgccaactg gcggctaaaa aagaacgtgc gcgatgttta
     9481 tcgtattttc ccgtctggct gttatacacc ccttcatcac gaataccggt tgttgaatca
     9541 gtccataaaa atactcgtga acgccacatt ccccacagat caatatttgg tgttaaagcc
     9601 caataaccta atgctccttc agaaactgtc ggtaatgtac gttgcgaact gccagccatt
     9661 aacggaatac gataaggtaa gctaccataa ctataatccg tcccgaagcg acctaattta
     9721 agcaggctat tttcactggg ttttattttt actgccccca gggcaaagcc aaaactttta
     9781 tcaccatcaa gataccaccg gatactcata tcagcacgag cacctaattt ataaacataa
     9841 tacgctccgc cttcaacgcc aataatattg gaataataac cactattgaa atcgagcaat
     9901 cctccctgta cccatgcgta aatatctctt cccgcgccag cactgggctt atcagccctg
     9961 cgcaattcgt ttttcagtct gatgcgtagt cccgcttcat cgtcagccag ttgcgccacg
    10021 taagcagagg taaggccaga ggcagccgtc aggcaaataa cggccatggc cactattttt
    10081 ctcatagttg cacctttgat gttctgatta tttttattga atattaatta gtgatatcgc
    10141 tgattaattg ctgctgcact tttttacgat tatcaatttc aaccacgatt tctttgaatt
    10201 ttttatccgt gagcggataa aaccagataa taacgaatgc cagtagcata aatccgcaag
    10261 gtactaaggc aattgatgtg cggatgccca taataacttc cggcgtttac acctgattgg
    10321 cgatatatcc gcttaatccc aaaataaagg caggaattga acctccgatt gcctgaccgc
    10381 atttacgggt aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt
    10441 attcaccgta ttctacggta tcagcttcca gtgcccacat cacagtcata gtaacgccct
    10501 gaccaattga agcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc
    10561 agaagaatag cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt
    10621 taccgatcct cgcgaccatc cccggcacaa gcggtgccga tgccacagta ccaaccaggt
    10681 tttgcaccag taccagcaca gtgaacagcc cggtatcatt taacacatag cgcacgtaga
    10741 acaacgacga ggcgctgacc gcgaaggtcg aaatcagcac acacagcgca ccgatgcaca
    10801 acataaacag cgggcgattc cgtttcaggg tttgcaaact gatcttcaat gacggctgcg
    10861 ccacgatacg taccacattt tcacgcgtcg atttgaagca gatgaagtaa agcaccattc
    10921 cggcaatcgc cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg
    10981 agttcttaat gctcggtcct atcagaaatg ccaggcatac aaaggtcaat gaagcggcaa
    11041 tcccacgagc cgcgcccaga cgggcgcgag attgtggttg ttgggtcatc gcggtagcaa
    11101 gtgaaccata aggaatattc accaggctgt agcaaagccc gaggcccatg taggtcaaat
    11161 atgcatacac cactttgcta ccatggctcc agtcggtcgg tacccagaat accagcacgc
    11221 tgaagatcat taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccagcggg
    11281 tattcacact gtccaccact cgtccggcaa agacgtcggc gaaggcatca aatacccgca
    11341 ccagtaacag catggtgcct gccgcagcgg caccgacgcc agcgacgtcg gtgtagtaac
    11401 tcaataggaa gagcgccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt
    11461 agccgacgat ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt
    11521 ttcaccgaag ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt
    11581 gcggtcgcga gtgaagatcc ctttcttgtt accgccaacg cgcaatatgc cttgcgaggt
    11641 cgcaaaatcg gcgaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac
    11701 gcggtgatac atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat
    11761 tgagtgcagg ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag
    11821 tttctcctgc caggccagca gttctttttc cagtaccttc tctgccgttt ccaaatcgcc
    11881 gctttggaca taccaaccgt aataacggtt caggcacagc acatcaaaga gatcgctgat
    11941 ggtatcggtg tgagcgtcgc agaacattac attgacgcag gtgatcggac gcgtcgggtc
    12001 gagtttacgc gttgcttccg ccagtggcgc gaaatattcc cgtgcacctt gcggacgggt
    12061 atccggttcg ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgctat
    12121 cagctcttta atcgcctgta agtgcgcctg ctgagtttcc ccgttgacag cctcttcgct
    12181 gtacagttct ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc
    12241 gacagctgca gtttcatcaa tcaccacgat gccatgttca tctgcccagt cgagcatctc
    12301 ttcagcgtaa gggtaatgcg aggtacggta ggagttggcc ccaatccagt ccattaatgc
    12361 gtgatcgtgc accatcagca cgttatcgaa tcctttgcct cgcaaatccg catcttcatg
    12421 acgaccaaag ccagtaaagt agaacggttt gtggttaatc aggaactgtt cgcccttcac
    12481 tgccactgac cggatgccga cgcgcagcag gtagatatca cactctgtct ggcttttggc
    12541 tgtgacgcac agttcataga gataaccttc acccggttgc cagaggtgcg gattcaccac
    12601 ttgcaaagtc ccgctggtgc cttgtccagt tgcaaccacc tgttgatccg catcacgcag
    12661 ttcaacgctg atatcaccat tggccaccac ctgccagtca acagacgcgt ggttacagtc
    12721 ttgcgcgaca tgcgtcacca cggtgatatc gtccacccag gtgttcggcg tggtgtagag
    12781 cattacgctg cgatggatcc cggcatagtt aaagaaacca tggaagtaag actgcttttt
    12841 cttgccgttt tcgtcggtaa tcaccattcc cggcgggata gtctgccagt tcagttcgtt
    12901 gttcacacaa acggtgatac gtacactttt cccggcaata acatacggcg tgacatcggc
    12961 ttcaaatggc gtatagccgc cctgatgctc catcacttcc tgattattga cccacacttt
    13021 gccgtaatga gtgaccgcat cgaaacgcag cacgatacgc tggcctgccc aacctttcgg
    13081 tataaagact tcgcgctgat accagacgtt gcccacataa ttacgaatat ctgcatcggc
    13141 gaactgatcg ttaaaactgc ctggcactgc aattgcccgg ctttcttgta acgcgctttc
    13201 ccaccaacgc tgatcaattc cacagttttc gcgatccaga ctgaatgccc acaggccgtc
    13261 gagttttttg atttcacggg ttggggtttc tacaggacgt aacataaggg actcctcatt
    13321 aagataaaaa tactggtcaa cctttaatct gattagttaa ccaattctgt gttgcacaca
    13381 gttatctgtg agaaacccat ggaagagaaa tggaaataaa aagaactgac accgcgatca
    13441 aaaaaccaaa aactgcaata cccacagcgt gatatggatc gcattaatct ttaaaacagc
    13501 gttgacagaa gccataaaac aagcaatcga gcgttcgttc gagagtattg ggaacgataa
    13561 actctcatga catatggcta cggtgaatat tttggggggg aattctccga acacggggag
    13621 gataattttt gcacagagaa attacgatat gaaacgcggg cacagttatc accagcccgc
    13681 gaaatgagag gatcaggatg cggttaatat accgccaatc atcgcgcgta atccctgagc
    13741 aaacgacgct tcacgcgcca ggtcttccgc ttcgatatcg gccagtacac cgtaggtcag
    13801 atccagcagt aaacgtgaag tcattgccgt gttgatgtct gggcctatct cgccttttcg
    13861 ctgggcatca cg
//
LOCUS       DAJTYF010000104        13777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTYF010000104 DAJTYF010000000
VERSION     DAJTYF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.104"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(194..1447)
                     /locus_tag="OO590_003151"
                     /pseudo
     CDS             complement(194..1447)
                     /locus_tag="OO590_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1554..2447
                     /locus_tag="OO590_003152"
     CDS             1554..2447
                     /locus_tag="OO590_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6638430.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2582..3802
                     /gene="mlc"
                     /locus_tag="OO590_003153"
                     /gene_synonym="dgsA"
     CDS             2582..3802
                     /gene="mlc"
                     /locus_tag="OO590_003153"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6638431.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3927..4622
                     /gene="bioD"
                     /locus_tag="OO590_003154"
     CDS             3927..4622
                     /gene="bioD"
                     /locus_tag="OO590_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6638432.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4575..5867)
                     /gene="clcB"
                     /locus_tag="OO590_003155"
     CDS             complement(4575..5867)
                     /gene="clcB"
                     /locus_tag="OO590_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6638433.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6026..6640)
                     /gene="dmsD"
                     /locus_tag="OO590_003156"
     CDS             complement(6026..6640)
                     /gene="dmsD"
                     /locus_tag="OO590_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6638434.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6683..7537)
                     /locus_tag="OO590_003157"
     CDS             complement(6683..7537)
                     /locus_tag="OO590_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6638435.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7539..8156)
                     /gene="dmsB"
                     /locus_tag="OO590_003158"
     CDS             complement(7539..8156)
                     /gene="dmsB"
                     /locus_tag="OO590_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6638436.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8167..10437)
                     /locus_tag="OO590_003159"
                     /pseudo
     CDS             complement(8167..10437)
                     /locus_tag="OO590_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(10446..11112)
                     /locus_tag="OO590_003160"
                     /pseudo
     CDS             complement(10446..11112)
                     /locus_tag="OO590_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            11095..12695
                     /locus_tag="OO590_003161"
                     /pseudo
     CDS             11095..12695
                     /locus_tag="OO590_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397936.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            13189..13557
                     /locus_tag="OO590_003162"
     CDS             13189..13557
                     /locus_tag="OO590_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638437.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
BASE COUNT         3453 a         3513 c         3520 g         3291 t
ORIGIN      
        1 tagaataact gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt
       61 acttataatc ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt
      121 aggatggcta ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt
      181 ccggacttat ttttcaggcg tgcagacgac gatgcaaacg cgccccgacc agcagagcaa
      241 tgaccagcat cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa
      301 caccacccag cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg
      361 aggcctggcc tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg
      421 ctgcgaagaa tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa
      481 ataaggtcat cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc
      541 gcccatagcg ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag
      601 cccgcgataa taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat
      661 agccgatgta attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca
      721 ataacggtaa tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc
      781 gcagcgaagt cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgccg
      841 aggccagcgc gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga
      901 agacaccact aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc
      961 ccatcaaaaa ggccacgaaa ctggggggga tttcctcgct aagataagtc atgccaacag
     1021 ctgccacgcc acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc
     1081 tggtcatcat tgtcgaaagt aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca
     1141 ctggtttgcg accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca
     1201 tcgccgtgga aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg
     1261 aaagcaccgg aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag
     1321 agaacagcgc cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg
     1381 gctgagaaat gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac
     1441 ggctcacttg aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa
     1501 aatcgaattg ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata
     1561 ttgaacttcg tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg
     1621 cggctgcccg cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc
     1681 aacaaatcgg tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag
     1741 gaaaacagtt tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc
     1801 gtgctgaaag actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg
     1861 ctccttttat tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcctgatg
     1921 tccatttaca aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa
     1981 cgctggatat gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca
     2041 tcgtccatga accgcttatg gcgatgatcc cgcacgatca tcccctggca aataatccga
     2101 gtgtaacact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
     2161 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
     2221 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
     2281 caattttgcc tgcgtcattt aaacgtgttc agctcaatga aatgcgctgg gtgccgattg
     2341 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
     2401 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
     2461 gaaaaagccc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
     2521 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
     2581 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
     2641 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
     2701 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
     2761 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
     2821 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
     2881 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaattgg cgttaaaaga
     2941 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
     3001 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
     3061 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
     3121 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
     3181 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
     3241 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
     3301 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
     3361 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
     3421 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
     3481 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
     3541 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
     3601 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
     3661 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
     3721 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
     3781 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
     3841 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
     3901 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
     3961 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
     4021 aaacggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
     4081 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
     4141 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
     4201 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
     4261 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
     4321 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
     4381 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
     4441 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
     4501 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
     4561 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
     4621 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
     4681 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
     4741 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
     4801 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
     4861 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
     4921 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
     4981 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
     5041 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
     5101 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
     5161 atcagcgcat agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg
     5221 tcgctatgat taatcagatt gctaaccagc aatgccacga cggcggaaat aatcaccggg
     5281 ccgagagagg ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca
     5341 agcggggcac gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc
     5401 cattcctggc gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga
     5461 atcatcgcac cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc
     5521 gatttaacca ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa
     5581 tcggtcggcg catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc
     5641 gccgccagtc cgccgagcgc cggagttagc agccgtcgcc agggggaaag gtttgtcgct
     5701 gcattgacca gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca
     5761 tgacgaaacc cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga
     5821 cggcggaaca tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg
     5881 caatgaaaca cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc
     5941 tttaagagat aaaaaaacgt gacattgtaa cgtattattg ccgaatcgcg cgtaatcgtc
     6001 accatccggc aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca
     6061 acagttgtga ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga
     6121 aagggtgttc tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt
     6181 gccatgccag caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca
     6241 tcaacagcag tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc
     6301 ctttctcgcg catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc
     6361 gatccagcca gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac
     6421 gctgccaggc ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg
     6481 ctaatgacgc ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa
     6541 ccagaggtgc ggcttccgca ctctctggag cgtaataaaa cagcgctccc aacacgcgcg
     6601 ccgcgacaga aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg
     6661 cgggttgccc cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc
     6721 cataaaagag cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa
     6781 gtccggcaac gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg
     6841 cgcataacaa taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg
     6901 cttgttgcac ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg
     6961 cacaagcgat cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc
     7021 gggctgcccg cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga
     7081 aagccagcgt agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg
     7141 tcatcatcca gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca
     7201 acgcttgcgg cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa
     7261 atatggaacc ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt
     7321 taaacgctcg cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca
     7381 acagccagag gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg
     7441 cgaaccagcc aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa
     7501 agataaccag tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc
     7561 agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg
     7621 ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag ccagagtacc gtgcttctgg
     7681 cgcagctctt caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg
     7741 ggttgtttcc cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt
     7801 tcagcattgt actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata
     7861 caaacatctt catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg
     7921 cagacttttg tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg
     7981 aacacgttct ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa
     8041 atacggcgga aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag
     8101 gttttacagc cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc
     8161 tactccttaa accttttcga tctggacaag attgctgtgc gacgggtttc cctttgctag
     8221 cggtgaaggg cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc
     8281 aaacatatcc gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg
     8341 cggagtcact tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc
     8401 gccatgacgg ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca
     8461 ggcctgctgt aacacatcaa tattgccgta gctggagtgg gtacgcgctt tgtagtggaa
     8521 gcccgttaac tgcagtggat aggttttccg caggggagcg tcccagccat caaaacctga
     8581 ggtatacgca ggaatgggat gaataatttc atcttttttc aattcccagg tatctgcaat
     8641 cttcgccagt cgttcagaat aaatttcgat tttccccgaa ggtgttttca acgggtttgc
     8701 ctgtggatct tcacggaatg cgcggaaagc gacgtagtgt tcttccgggc attttttctt
     8761 aaagatcccg gtcgttttca tctcctcgta gtcgggcatc tcagggttac gttccttcgt
     8821 tttcgcatgg agatatttga tccattcatg ctgactgcga ccttcagtaa aggtttgata
     8881 aacgtctggt cctaagcgtt tggcgacttc actcagcatc cagtagatgg gtttgcgttc
     8941 aaattttgct gaggttgcgg gttgggcgag gatcacatag cccatattcc ctgcagattc
     9001 atgagagata aggtcttctt gctctgttgg catcaggtca ggcaacagga tatcgcaata
     9061 cttagccgag gccgtcatga agtggtcaat gccaacaatc atctcgcact tgctgtcatc
     9121 ctgaagcacc tcatgggtgt gattgatgtc gccatgttga ttgatcaatg tgttactggc
     9181 gtagcaccat aaaaacttga tggggacatc cagtttttct tttccacgaa caccatcacg
     9241 ggtcgcggtc attttcgtac catggtcgat ggcatctgtc catgtaaaaa cggaaatctg
     9301 cgttttaaca ggattctcga gcatcgggaa ccattctacc cccagatccc agctaccttt
     9361 gcgtacacct gagttgccgc cgtttatgcc gacgttaccg gtaagaacgg aaagcatggc
     9421 aatagcgcgg gacgtttgtt cgccgttgga gtgtcgttgt ggcccccaac cctgacaaat
     9481 ataagcaggt tttgctgaac cgatctctcg tgccaactgg ataatttttt ctgccgggat
     9541 gctggtgatt tttgctgccc attccggcgt tttagctatg ccgtcaggcc cttcgcccag
     9601 aatataggct ttataatgcg cgttacgtgg tgcgttggcg ggcagcgttt tttcatcgta
     9661 accaacacaa tatttgtcga gaaatggctg atcgaccatg ttttcagtaa tcagtaccca
     9721 ggcaatcgca caggccagtg cgccatcggt gccagggcga atgggcagcc attcatcttc
     9781 acgccctgca gctgtgtcgt tatagcgtgg atcgatgacg atcatgcgtg cgtttgaacg
     9841 ttcgagggct tgctcgacgt agtaagtgac accaccgccg ctcatccgcg tttctgccgg
     9901 gttatttccg aacataacga ccagtttcgt attggcgata tcatccgggc tgttgccatc
     9961 attggcaccg aacatataac tcattgcggc actgatctgt gcggtactgt agctgccata
    10021 gcgactgaga aaacccccgc aagagttcat cagacggtac gggacgttcg agttggtgat
    10081 atttccgcca tctacgcctg ttccgtacag gacatgtaca gcctcattgc cgtaatcttt
    10141 caggatccgc cgaagattat cactgatggt atccagggct tcgtcccaac ttatccgttc
    10201 aaatttacct tcaccgcgct cgccgacgcg cttcatggga tatttcaacc tgtcaggatg
    10261 attcatccgt cggcggatag agcgcccgcg taaacatgcc cgaacctgat gattaccgta
    10321 aacgtcgtca cctgtcgtat cagactccac ccagtacacg gtgtcatctt tcacatgcaa
    10381 acgtagcaga cagcggctcc cgcagttaac ggtgcaggaa ctccagaccg ttttctcttc
    10441 tacccatctt tttccagttc ccaggtacgg gcaatttccg ccagcttgct ggaataaatt
    10501 tcaatcttac cggaaggcgt tttaagtgga tttgcctcgg ggtcgtcacg aaatgctttg
    10561 taggcgacaa aatggccatt gggatcttta cgcttataga tacccatttt tttcagttcg
    10621 tcgtaagacg gtaacgccgg atctttggca agcattttgg cgtacagatg ttgtaaccat
    10681 tgttcctgcg tgcgaccttc tgtgaacttt tgatagacgt caggtccaag acgtttcgcg
    10741 acttcactca ggatccagta aatcggttta cgttcgaatt tttcgctggt gactggctgg
    10801 aggaaaatga gatatcccat gttaccggcg tagtcgttag gaataatatc ttcctgctca
    10861 acggtcatca ggtctgccag cagaatgtcg gcatattttg ccgtatgagg tcataaagtt
    10921 ttcgatgacc acaatcattt cgcatttcga ttcgtcctgc agaatttcat gcgttttgtt
    10981 gatgtcagaa tgctgattaa cgagggtatt tcccgcgtag ttccagatgg actccactgt
    11041 cagacggatg gatggacttg aagtagttga ctcaacgcta cttaaggtcc tgatatgact
    11101 gacattaacc atgataatac tgtatatgca tccagttctt tctctgggtc tccctatctg
    11161 tatcagcaag tctacacctc aagtcctgac acaaggctga cacaaatcaa ggaccttgca
    11221 gtcccgcact tgctcgcaag gctcgctacg ccacagttag tccgtagagc taacggtcga
    11281 tatactatcc gcttcagact caagggacag accacaccgt ttctttcagt atcgacccgc
    11341 agcacagaca ggagagtagc cacaatgcgt cagagagagc ttgcagccac cgctaaggcc
    11401 ttcatgttgg ataggcctga agtctcacta caggagctta cagagcatct ccggtctatg
    11461 gctgagcagt tcctgacaga cgctagtgac gattactgga atggacttga ggtcgctact
    11521 ttggtggacg agaagtccaa cctgagggag ctagccgcta cgcaagcctt gagtctggac
    11581 cagcagaagg gcataaggtt agcgcttgaa gtcctgaccg cagcacagca gagggtagac
    11641 actggtgata cctctggtct gattaagctg attgatgata ataaccatac tgattactca
    11701 actataggcg actctacgtc aattctgaag aatgagcagg gggatagcca gcagttttca
    11761 cacaggagcg ccaaccatct gtagtttttt caagcctagt gtcctcgctg ctggctgaga
    11821 aggcccagac cctgaagtca tccagctaca aggacctgtc gtcctcactg aataccgtct
    11881 ctcggttctt acctgaagac atggacctga tgtcccgctc tgagtggctg gctgtcaggg
    11941 acgctatgct ggcttctgag gtgagaccgt cgacgattaa caagctgctg actaaggcga
    12001 agatgtgtct tgattacggt ctgatgaatg ggcaacttga gggccgcaac cctattgaac
    12061 ggatgaagct gaccaaggat atcgactcca agcgcagagc gttcacagac gaggagctgg
    12121 agagacttct ggtccgtgtt gaggctgagt atcagttcac acgacacacg gctcacacga
    12181 cctctgaggc ccgtagatgg gcgtctctgg tttctgtagt tactggcgct cggtctgctg
    12241 aggtctgcca cctgactaaa cgtgacattg taaccttaga caatggactg gtctgtatcg
    12301 atataaacga agatggggac ggtaagtctg tgaagaacaa gcattcagtc cgacttgtgc
    12361 cgctgaccga tggcgttcat gggttcgacc ttgagtcttt cctcaaatgg gtagacatgc
    12421 agcctgatga tggtcctctc ttcgggatga ctccgagcgc ctactctagc tggtttaact
    12481 caagggtcct gactgaggcc ctaggagact ctcaggatgt ctctctccac agtctgagac
    12541 attggctggc gacccgaatg aaagagcgtg gagtcaatct ggtggacgct caaggcatct
    12601 taggtcacag cagtcagtca attacgtatg acctgtacgg gaaaggacac gctgtgggta
    12661 ggctggctga tgtgcttaag acagctttac tttaagtagg gagacccaag gtggagacca
    12721 tatacggctt gatggttgag accttgggtc actgaagtct gactgaagtt agggagggtg
    12781 ataataatca taatccctcc tgactatagg cccactggtc cgccttcggc tgtccgactc
    12841 taagtctgtc tgtggtctta tttaaactgc gtcctaaact ccagtccctc caaattccta
    12901 tggtctcctc ttgacattcc tgtttaactg tgagtcgcct ctggtctctc tttatatata
    12961 cttccttaat tcctgaggcg tctttagtcc ttgacatttg gttcaacctg tgagtcacgt
    13021 ccggtccccc tctttatatt tacttcctta attcctaggg tcgccttaat ccgtgacatc
    13081 cagtccagcc tgtgagtcac atcctgtcgc gctactgtat gcatcaccag tgatggactt
    13141 gattatcact ataggggaac caactaattc aactacagga gatactctat ggcacgacca
    13201 atcgcaactc acgacaacac gttcaccaaa gcctacttgc agcagcactg cggggacctg
    13261 ctgtcattcg atggtcaggg cgacctgagt ggttggctgg atgatgtcct tactggtgct
    13321 ggtcgcctga acgaatcaat ggcaagcaat accaagcctg tcagcccata cttgattctg
    13381 acccaactac taacgcacga cacattaact gtaagcgctg tgcaggagtc cctgagccgt
    13441 aagcgtgttg ccttgggtga acctatggtt tcaactcgct acgctcgcta cgtctatgct
    13501 accgtcgtca gcgccagtaa atccgtccag taccacgcca gtaaagctgg ttcatgatta
    13561 cggaccctaa actatcacta tagggagacc agcctccgcc tctgagtctg atgcgtcaga
    13621 acatgcgagc atctggtcaa ccgacattaa gtatctctcc taagtaatca aggcatgtga
    13681 ccgcctctgt cagacctcgt gtatcgacct ccagctcgtt agcgttcttc agtcatctca
    13741 gttagtagct cgtctactca ctacattggc ttaagtc
//
LOCUS       DAJTYF010000105        13724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTYF010000105 DAJTYF010000000
VERSION     DAJTYF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.105"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            262..1515
                     /gene="kch"
                     /locus_tag="OO590_003163"
     CDS             262..1515
                     /gene="kch"
                     /locus_tag="OO590_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6638438.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1570..1743)
                     /locus_tag="OO590_003164"
     CDS             complement(1570..1743)
                     /locus_tag="OO590_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6638439.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1886..3346
                     /gene="cls"
                     /locus_tag="OO590_003165"
     CDS             1886..3346
                     /gene="cls"
                     /locus_tag="OO590_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6638440.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3381..3710
                     /locus_tag="OO590_003166"
     CDS             3381..3710
                     /locus_tag="OO590_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6638441.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3763..4767)
                     /gene="oppF"
                     /locus_tag="OO590_003167"
     CDS             complement(3763..4767)
                     /gene="oppF"
                     /locus_tag="OO590_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6638442.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4764..5777)
                     /gene="oppD"
                     /locus_tag="OO590_003168"
     CDS             complement(4764..5777)
                     /gene="oppD"
                     /locus_tag="OO590_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6638443.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGAATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5789..6697)
                     /gene="oppC"
                     /locus_tag="OO590_003169"
     CDS             complement(5789..6697)
                     /gene="oppC"
                     /locus_tag="OO590_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6638444.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6712..7632)
                     /gene="oppB"
                     /locus_tag="OO590_003170"
     CDS             complement(6712..7632)
                     /gene="oppB"
                     /locus_tag="OO590_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6638445.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7718..9349)
                     /gene="oppA"
                     /locus_tag="OO590_003171"
     CDS             complement(7718..9349)
                     /gene="oppA"
                     /locus_tag="OO590_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6638446.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10087..10734)
                     /gene="ychE"
                     /locus_tag="OO590_003172"
     CDS             complement(10087..10734)
                     /gene="ychE"
                     /locus_tag="OO590_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6638447.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11211..>13724
                     /gene="adhE"
                     /locus_tag="OO590_003173"
                     /gene_synonym="adhC"
     CDS             11211..>13724
                     /gene="adhE"
                     /locus_tag="OO590_003173"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6638448.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFD"
BASE COUNT         3587 a         3396 c         3193 g         3548 t
ORIGIN      
        1 gtgttcttgt ttggcctttc cttatagccg gtcagtgcat tcactaccac aagcagataa
       61 taactttatc tgtatttctt tatatgatgt gagcatgttc aggaaaaaag ccctttcctg
      121 acgcagagtt gctcactcaa aacctttgat tactgcggat aaagtcatct actattgttt
      181 aacacattca cgcgctgcat gtatgttcag aaaatgtgca cgctaagata attaaacaaa
      241 attgtggtta cagaggtatc agtgagtcac tgggctacat tcaaacaaac agcaacaaat
      301 ttatgggtga ccctacggca cgacattctc gcgctggccg tctttttaaa tggattgctt
      361 atttttaaaa caatctatgg tatgtcggtc aatttgcttg atattttcca tatcaaagca
      421 ttttcagagt tggatctctc cttgctggca aacgccccac tatttatgct cggcgtcttt
      481 cttgtcctga actccattgg cttactgttc cgggcaaagc tcgcatgggc aatcagtatc
      541 attttgttgt tgatagcgct aatttacacc ctgcattttt atccctggct gaaatttagt
      601 attggatttt gcatttttac gctggtgttt ttgctgatac tgcgcaaaga cttctcccac
      661 agtagcgccg cagccgggac aatttttgca tttattagtt tcacgacatt actgttttac
      721 tcaacctacg gtgcacttta tttaagcgaa ggttttaatc cgcgaataga aagtttgatg
      781 accgcgttct atttttcgat agaaaccatg tcaaccgtcg gctatggaga tattgtccct
      841 gtttctgaat cagcacgatt gttcactatt tcggtcatta tttccggcat taccgttttt
      901 gccacatcta tgacctcaat ttttggtccg cttatccgcg ggggattcaa caaactttta
      961 aaaggaaaca atcatacaat gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt
     1021 ctcgccatca atacgatcct gcaactgaat caacgcggac aaaacgtaac ggttatcagc
     1081 aacttgcctg aagatgatat caagcaactt gagcaacgct taggcgataa cgctgatgtt
     1141 atccccggtg acagtaatga cagttcagta ttaaagaaag cgggaatcga tcgttgccgg
     1201 gccattctgg cgctgagtga taacgatgca gataacgcgt ttgttgtact ctcggcaaaa
     1261 gatatgagca gtgatgtcaa aacagttctc gccgtcagtg atagcaaaaa cctgaataag
     1321 attaagatgg tacatccgga tatcattctc tcaccgcaac tgtttggcag cgaaattctg
     1381 gcgcgagttt taaatggtga agagattaat aatgatatgc tcgtttcaat gttgttgaac
     1441 tcaggtcatg gtattttcag cgataacgat gaacaagaaa cgaaagctga cagtaaagaa
     1501 tcggcgcaaa aatagagttt aacgacggaa gagtaaactt attgggcacc gcaatggtgc
     1561 ccagtacatt tagatattgt agatcgcaaa caataacgag ttacgctgtt tgtttagaat
     1621 gcacttcctg atgctgtgga tacgcatatt tcggcgcgat tgcccctcaa gccaacgcga
     1681 tttacgtcgg ctagcctgac gctgcatgcg ccagcgcccc acttccgttc tactccgctt
     1741 catgttaact actctatgca ataacagaat ggtcattata cccccgcccc cgacgctgac
     1801 cagtgctttt cccgcgtttt tatttgccag atgaatccat atgcgtaaac tcataacaat
     1861 gcgctttcaa aaggatttct aatctatgac aaccgtttat acgttggtga gttggttggc
     1921 cattctggga tactggttgc tcattgcagg cgtaacttta cgcattctaa tgaaacgacg
     1981 cgcagttccc tccgcgatgg cctggctgtt gattatttac attctgccgt tagtcggaat
     2041 tattgcctat cttgccgttg gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag
     2101 agcgatgtgg ccttccaccg caaaatggct taacgacctt aaagcctgta agcatatctt
     2161 cgccgaagaa aatagcagtg tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg
     2221 gatcgctggg gtcaaaggga atcagctaca actgatgacc gagtcagatg atgtgatgca
     2281 ggcgttaatc cgcgacatcc agctcgcgcg ccataatatt gagatggtgt tttatatctg
     2341 gcagcccggc ggcatggcgg accaggtggc tgaatcatta atggcggctg cacggcgtgg
     2401 tattcattgc cgattgatgc tcgactccgc cgggagtgtg gcttttttcc gcagcccgtg
     2461 gcccgagcta atgcgtaatg ccggtattga agtggtcgaa gccttaaagg tcaatctgat
     2521 gcgtgtgttt ttacgccgta tggacctgcg ccaacatcgc aagatgatca tgatcgataa
     2581 ttacatcgcg tacaccggca gcatgaatat ggtcgatcct cgctacttca aacaagatgc
     2641 gggcgtaggg caatggattg atctgatggc gcgtatggaa ggccccatcg ccaccgcgat
     2701 ggggattatt tattcctgcg actgggagat tgaaaccgga aaacgtattc tgccgccacc
     2761 accagatgtc aatattatgc cgtttgaaca ggccagtggt cacaccattc acacaattgc
     2821 ttctggcccc ggctttccgg aagatctcat tcaccaggca ttattgactg cggcttattc
     2881 ggcgcatgaa tatttgatca tgaccacgcc ctactttgtg ccaagcgatg atttacttca
     2941 tgcgatttgc acggcggcgc agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa
     3001 tgactcgatg ctggtcggct gggccagtcg cgcattcttt acggaactgc ttgctgctgg
     3061 ggttaaaatt tatcagtttg aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg
     3121 cgaactaagt ctggttggca ccgttaacct tgatatgcgt agtctgtggc tgaatttcga
     3181 gattaccctg gcaatcgacg ataaaggttt tggtgcagac cttgccgccg ttcaggacga
     3241 ttatatttcg cgttcacgtc tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca
     3301 acgtgtcgcc gagcgactgt tttacttctt cagcccgttg ctgtaaaacg tcgccaacag
     3361 aggttaaaca ggtagtgact atggatatgg atctaaacaa tcgcctgact gaagatgaaa
     3421 cgcttaagca ggcctacgat atttttctcg aactggctgc cgacaatctc gatccagctg
     3481 atgttctgct gttcaatctt cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg
     3541 cggaagactg gcaggaacat gttgatttcg atttgaaccc ggactttttt gccgaagtgg
     3601 tgattggtct ggcggacagt gaagatggtg aaatcaacga tgttttcgcc cgcattctgt
     3661 tatgccgcga aaaagatcac aaactttgcc atatcatctg gcgggaataa ataatcgctt
     3721 ttagcgtgaa aaagggctga caactgtcag cccttattgt ttttaaagcg gatcgacttt
     3781 caggcaagaa acggcgtgtc tgaaactccc ctccagaaca ggacgtgttt tggcgcactc
     3841 cggaccggca atcgggcaac gggtacggaa aacacaaccg gaaggcgggt tgatcggcga
     3901 cggtaattcc ccttccagta actggatggt tttgttcttc tccagatccg gatcaggtat
     3961 ggggactgcc gacatcaatg ccctggtgta aggatgtagt ggattgtggt agacctcatc
     4021 ataggtcccc agttctactg catggccgag atacatcacc aacacacgat cggaaatgtg
     4081 ttttaccacg gccaggtcat gagcaataaa aattaatgac aatcccatct cacgttgcag
     4141 ctgctggagc aggttgacca cctgcgcctg aattgacacg tccagcgccg aaaccggctc
     4201 atcgcagata atcagcttcg gttcaagaat aagagcacga gcaatcccga tacgctggca
     4261 ctgcccaccg gagaactcat gcggatagcg gttaatcagg ttaggcaata acccgacttt
     4321 cagcatcatc gccttcacgc gctcgcgaac ttcctggcgt gacattttcg gatgataagt
     4381 acgcagtggt tcagcgatga tctcgccgat ggtcatacgc gggtttagcg atgccaacgg
     4441 atcctggaaa atcatctgaa tatcactgcg aacggcacgc cattcatcgg gcttcatgcc
     4501 cagcaactct ttacctaacc aggcaacatg accgtcggtc gccttgacca aaccgatgat
     4561 ggcgcgagca aaggtggact taccgcatcc cgattcccct accacaccta atgtttcccc
     4621 ttcatacagg cgaagagtga caccatcgac ggctttgagc gttttcggcg gttgccagaa
     4681 ccactgtttg ccatctttga tttcaaagtg cactttaaga tcggcgattt caaggaggac
     4741 ttttcttcct tcagttacag cattcataac agttcctcca ccggtttaaa gcaagcacgc
     4801 agacggccag gcgtaaactc ttccagcggc ggtgcgctac tacaaatttc catcgcatgt
     4861 ggacaacgtg gctggaacgg gcaacctttc ggtaatcgca gcaagtttgg cggattaccc
     4921 gggatggtca acattgtttc accttccgca tcaagacgcg gcaccgcgtt gagcaaaccg
     4981 atagaataag gatgaacggg ttgataaaag acatcgcgcg cattgccata ttccatcgtg
     5041 cgcccggcgt acattaccag cactttgtca cagatccccg ccaccacgcc aagatcgtgg
     5101 gtgatcataa tgatggcagt attaaattcc cgcttcagtt cattcaatag cgtcatgatc
     5161 tgcgcctgta cggtgacgtc cagcgctgta gtgggttcat ccgcaatcag caacttaggt
     5221 cgacatagca atgccatcgc aatcatgact cgctgacgca tgccgccaga aaattcgtgc
     5281 gggtacattt tcatgcgttt acgtgcttcc ggcattttta ccgcatcgag catccgcacc
     5341 gactcttcaa acgcctcagc tttgctcatg ttcttatgca gcatcagcac ttccatcaac
     5401 tgctcaccga cgcgcatata gggattcaac gaagtcattg ggtcctgaaa aatcattgag
     5461 atttgttcag cgcgcagttt attgagttca cgctctggca aattgaggat ttcacgccca
     5521 ttgaaggttg ccgctccgcc aatgcgtccg ttggcagcca acaggcccat caacgcaaat
     5581 gcagtttgcg atttacccga accagactca cctacaatgc ccagcgtttc tccggcacgt
     5641 agggaaaaat ttaaatcatt gaccgccgtg acgtcgccgt ccggggtact aaaggtgaca
     5701 cgcaaatctt tcacgttcag cagtgcgtca gcctgttgtt gtgcgagcgg cacagttgca
     5761 gtttcaatta cgctcatggc tgcactcctt aacgatcttt cgggtcgagg gcatcacgca
     5821 agccatcgcc gataaagttg aaacaaaaca gtgtcaccac aaggaatccc gctgggaaca
     5881 acagtaacca tggagagact tccatcgagt tcgcgccatc actcagcaat gccccccagc
     5941 tgcttaacgg ctcttgcgta cccaacccca ggaagctaag gaaagattca aagaggatca
     6001 tgctgggcac cagtagcgat gcgtagacca ccaccacacc gagtacgttc ggcacaatgt
     6061 ggcgaataac aatgcccggc gtcgatacac cgccaacttg tgccgcctca ataaactctt
     6121 tgcgcttcag actcagggtt tgcccacgca caatacgagc catatccagc caggaaacca
     6181 tgccaatcgc cacgaaaatc agcaggatgt tttggccgaa aaaggtcacc agcaaaatga
     6241 cgaagaacat gaatgggaag gagttgagga tttccagcag acgcatcatt accgaatcca
     6301 ctttaccgcc cagataaccg gaaagcgaac cataaagtgt ccccacgacc actgccacca
     6361 gtgccgcagc aacaccgacc atgagtgaga tacgcccgcc aatcgcaacg cgcacaagca
     6421 ggtcgcgacc ggatgagtca gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg
     6481 acatcatcgc ccagtcagta tcgtcatagg caaactggga aagcatcggt gccaggatta
     6541 caaataacgc gatcagcacc agcactatca gactggcaac cgccgcacgg ttatgcataa
     6601 aacgtcgacg tgcgtcctgc cacaagctgc gcccttcgac ctccagcttt tcactgaaat
     6661 tttccagcgt ctcgctgttt ttcttactta acatcatcgc gagctccagt atcagtaacg
     6721 gattttcggg tcgataaccg catatagcac atcgacaatg gcattaaaca aaatggttaa
     6781 cgcaccaacc aggatggtca ggcttaacac taaggaatag tcacggttca atgcgccatt
     6841 aacgaacagt tgcccaatcc ccggcaaacc ataaatggtt tcgatgacca tagaaccggt
     6901 aataatgccg acaaatgcgg ggcccatata ggagagcacg ggtaacagag caggttttaa
     6961 tgcgtggcgt aaaatgatcc gccgcatagg taaccctttc gcccgggcag tacgaataaa
     7021 gttggagtgt aatacttcaa tcatagagcc acgggtaata cgcgcaatac tggcgatata
     7081 ggcgagtgac aacgccacca tcggcaatat catgaattta agcgccccac cattccagcc
     7141 accgcccggc agccaatgca aaatgatcgc aaatatcatg actaataatg gcgcaaccac
     7201 aaaactgggg ataacaaccc cggtcattgc cagccccatc acggtatagt cccatttggt
     7261 gttttgtttt aatgcggcaa taacgccagc actaacaccc agtattaccg caaggaaaaa
     7321 tgcggcggct cccagtttgg cagaaacggg aaaactggat gccaccaggt cattgaccga
     7381 ataatcttta tatttaaacg atggaccgaa atcgccatgc gccagttgtt tcaggtagct
     7441 gaaatactgt gtcatgatcg gatcattaag atgatatttc gcttcgatat tggccatcac
     7501 ttctggcggt aaagtacgtt cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa
     7561 gaacgaaata gtaataagaa taaatagcgt cggaatcgct tccagacagc gacgtagaat
     7621 aaattttaac attgccctac cttctggcct gtgccttctg taatgcgata aaaatcagac
     7681 accgtggagc aggacactcc tgccccacgt attgccatta gtgcttcaca atgtacatat
     7741 tccgggtata ggtattatcc agcggatctt tgccggtata gccaccaacc cacggtttca
     7801 ccagacgcgc attcacgtag taataaacag gaacaatggc cgaatcctta tccagctgtt
     7861 gttctgcttt agtgtacaga gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg
     7921 ccataatgct gtcaaaggcc gggctcttat aatgcgcggt attcatcgag ctgttcgaca
     7981 gcatggtgtt caggaaggaa gttggttcgt tgtagtctgc acaccagcct gcacgggcca
     8041 catcaaaagt gccctggtga cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt
     8101 tgacgtttac accaatgttt ttcttccaca atgaagaggc agcaatcgcc agctttttat
     8161 gcagatcgga ggtgttatac cgcaggttga tggtcaacgg tttgtctgcg gtataacccg
     8221 cttcagccag cagttttttc gcttcttcgt tacgtttttc ctggctccag ccaaaccatt
     8281 ctggctgagt caattttgcg ccatcagtat acggtggagt gtaaccatag gcgggcatgt
     8341 cgccctgcgc tttcacttta ttaacaatga tatcgcggtc catacctagt ttcagtgcgg
     8401 tacgcacacg cacatcgttg aatggcggtt tctggttgtt aatttcgtaa tagtaagtgc
     8461 acaggtatgg atcaacgtga acttcgtccg ggatctcttt tttcagcttc tggaacaatt
     8521 cgatcggcat gttgttatac gtcatgtcga tttcaccact acggtagcgg ttgacatcgg
     8581 taacttcaga agcaataggc aaataggtta cctggttaat aacggttttc gcgttgttcc
     8641 agtaggtcgg gctgcgttca agaacgattc gttcgtttac gacccaatct tttaaggtat
     8701 aggcaccgtt ggtgacgata ttaccaggct gggtccattt ttcgccgaat ttctcgatag
     8761 cggcttttgg caccggtgaa gttgatgggt gaacaagtaa tttatagaag tacggaacgg
     8821 gttcacttaa ggtgacttct aatgtgtgat catcaatagc tttcacgccg agatcggtaa
     8881 tcggtttttt cccttcaaga atttcatcaa taccggcgat atgcccatat tgcagataac
     8941 tggcatacgg agaagcagtg ttcggatcaa cagaacgttg ccagctatac acaaagtctt
     9001 gtgctgtgac tggcgtgcca tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga
     9061 ctttcgcgtc tttattatcc caggattcag cgacgccagg tgctggatga ccgtcaagat
     9121 cgctgaccag taagccttca aacaggtctc ggctgatatt agactccgga acaccttcaa
     9181 ttttgtgcgg atctaatgac tgaacttctg aaccattgtt acgtaccagt gtttgttttt
     9241 ccgccagtgt gacgcctgcg ggtacatcag ctgccagcgc gacattccct gccattagcg
     9301 cagccagaac gccagctgct actaaacttc tcttggtgat gttggtcatt gttttttgga
     9361 ctccctcatt ataattactg gtattaacca gcatgtgtaa tcccggattg ggaccctgca
     9421 caggctcgga gactttctgc tgtcaggtta ctttatcgtt acgtattact atcaccgact
     9481 ttatttattc tggtgattca gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt
     9541 cgagacacat tattctttcg cgacgctctt taattgttaa ttattctcat taccaataaa
     9601 gaatcgtctg gcggttgccc ggaaagtacc aaatgcggtt cttactcgcc aatacatttt
     9661 gcaaaattgt taagcgattc tcttttattg accgtatcag aatctatagc ttgcatccat
     9721 gccttaacaa acatctttca attcaatcaa atagagattg gctaagcatg tctatctttt
     9781 ctgattacag ttccagtagt gaaatgcaca ataatttaac aattgattat tatttagcac
     9841 tttcctctac caaaggtagt ggaattacta atatcatttc aattattctt cagcaagccc
     9901 aggattatga tgtggcgaaa ataacatgat ggtgaggaga agcagttaag gaaatcctta
     9961 taactaactg taatttgaga agaatatttt tacctattcc ttttgctttt gataactgcg
    10021 cgggaacaaa gtaaaaataa tgacgaagta aacggagcat ggcagctccg tttcatttga
    10081 aaggaattaa ttaagcaggc cggggaaaat ccccttaata ccagtaacga taaattcaat
    10141 ccccaatgcc atcagcaata gccccataat acgcgtaatc acgttgatgc cggtctggcg
    10201 taaaacccgt accagccacg gtgccatgcg gaacaatccc cagcaacata aagcgaacaa
    10261 tgcaatagcc acaaagaaac caaacagata gctaatgctg tgataacgcg taccccagac
    10321 gatggtagaa ctgatcgccc ctggccccgc catcaacggc aacgccaatg gcaccacacc
    10381 aatactttca cgtaccgcgg tttctgattt ttcttgcttg ttctgtttat cctcgccaag
    10441 cttgccgctg atcatcgaca tcgctatcgt caccaccagg atgcccccgg cgatacggaa
    10501 cgaatcaatt gatataccaa aaagttgtag aatcgtgtcg ccgagaaaaa gcgagatcca
    10561 caagataatg gccacagaca ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt
    10621 ctgataactg gtcatgctga taaagacggg aataatccct accgggttga ccagcgcaaa
    10681 taacccgatg aaaaatttga agtaaacggg aaaatcaaaa agggtctgaa tcacggttag
    10741 ctccgaagca aaagccggat aatgttagcc ataaataagg ttgaaaagac gcgctgacaa
    10801 tacgcctttt gacagcattt ttcacctcct aactacttaa aattgctatc attcgttatt
    10861 gttatctagt tgtgcaaaac atgctaatgt agccaccaaa tcatactaca atttattaac
    10921 tgttagctat aatggcgaaa agcgatgctg aaaggtgtca gctttgcaaa aatttgattt
    10981 ggatcacgta atcagtaccc agaagtgagt aatcttgctt acgccacctg gaagtgacgc
    11041 attagagata ataactctaa tgtttaaact cttttagtaa atcacagtga gtgtgagcgc
    11101 gaataagctt ttgattttca tcggttaagc aaatcatcac cgcactgact atactctcgt
    11161 attcgagcag atgatttact aaaaaagttt aacattatca ggagagcatt atggctgtta
    11221 ctaatgtcgc tgaacttaac gcactcgtag agcgtgtaaa aaaagcccag cgtgaatatg
    11281 ccagtttcac tcaagagcaa gtagacaaaa tcttccgcgc cgccgctctg gctgctgcag
    11341 atgctcgaat cccactcgcg aaaatggccg ttgccgaatc cggcatgggt atcgtcgaag
    11401 ataaagtgat caaaaaccac tttgcttctg aatatatcta caacgcctat aaagatgaaa
    11461 aaacctgtgg tgttctgtct gaagacgaca cttttggtac catcactatc gctgaaccaa
    11521 tcggtattat ttgcggtatc gttccgacca ctaacccgac ttcaactgct atcttcaaat
    11581 cgctgatcag cctgaagacc cgtaacgcca ttatcttctc cccgcacccg cgtgcaaaag
    11641 atgcaaccaa caaagcggct gatatcgttc tgcaggctgc tatcgctgcc ggtgctccga
    11701 aagatctgat cggctggatc gatcaacctt ctgttgaact gtctaacgca ctgatgcacc
    11761 acccagacat caacctgatc ctcgcgactg gtggtccggg catggttaaa gccgcataca
    11821 gctccggtaa accagctatc ggtgtaggcg cgggtaacac tccagttgtt attgatgaaa
    11881 ctgctgatat caaacgtgca gttgcatctg tactgatgtc caaaaccttc gacaacggcg
    11941 taatctgtgc ttctgaacag tctgttgttg ttgttgactc tgtttatgac gcagtacgtg
    12001 aacgtttcgc aacccacggc ggctatctgt tgcagggtaa agagctgaaa gctgttcagg
    12061 atgttatcct gaaaaatggt gcgctgaacg cggctatcgt tggtcagcca gcctataaaa
    12121 ttgctgaact ggcaggcttc tctgtaccag aaaacaccaa gattctgatc ggtgaagtga
    12181 ccgttgttga tgaaagcgaa ccgttcgcac atgaaaaact gtccccgact ctggcaatgt
    12241 accgcgctaa agatttcgaa gacgcggtag aaaaagcaga gaaactggtt gctatgggcg
    12301 gtatcggtca tacctcttgc ctgtacactg accaggataa ccaaccggct cgcgtttctt
    12361 acttcggtca gaaaatgaaa acagctcgta tcctgattaa caccccggct tctcagggtg
    12421 gtatcggtga cctgtataac ttcaaactcg caccttccct gactctgggt tgtggttcct
    12481 ggggtggtaa ctccatctct gaaaacgttg gtccgaaaca cctgatcaac aagaaaaccg
    12541 ttgctaagcg agctgaaaac atgttgtggc acaaacttcc gaaatctatc tacttccgcc
    12601 gtggctccct gccaatcgcg ctggatgaag tgattactga tggccacaaa cgtgcgctca
    12661 tcgtgactga ccgcttcctg ttcaacaatg gttatgctga tcagatcact tccgtactga
    12721 aagcagcagg cgttgaaact gaagtcttct tcgaagtaga agcggacccg accctgagca
    12781 tcgttcgtaa aggtgcagaa ctggcaaact ccttcaaacc agacgtgatt atcgcgctgg
    12841 gtggtggttc cccgatggac gccgcgaaga tcatgtgggt tatgtacgaa catccggaaa
    12901 ctcacttcga agagctggcg ctgcgcttta tggatatccg taaacgtatc tacaagttcc
    12961 cgaaaatggg cgtgaaagcg aaaatgatcg ctgtcaccac cacttctggt acaggttctg
    13021 aagtcactcc gtttgcggtt gtaactgacg acgctactgg tcagaaatac ccgctggcgg
    13081 actatgcgct gaccccggat atggcgattg tcgacgccaa cctggttatg gacatgccga
    13141 agtccctgtg tgctttcggt ggtctggacg cagtaactca cgccatggaa gcttatgttt
    13201 ctgtactggc atctgagttc tctgatggtc aggctctgca ggcactgaaa ctgctgaaag
    13261 aatatctgcc agcgtcctac cacgaagggt ctaaaaatcc ggtagcgcgt gaacgtgttc
    13321 acagtgcagc aactatcgcg ggtatcgcat ttgcgaacgc cttcctgggg gtatgtcact
    13381 caatggcgca caaactgggt tcccagttcc atattccgca cggtctggca aacgccctgc
    13441 tgatttgtaa cgttattcgc tacaatgcga acgacaaccc gaccaagcag actgcattca
    13501 gccagtatga ccgtccgcag gctcgccgtc gttatgctga aattgccgac cacctgggtc
    13561 tgagcgcacc gggcgaccgt actgctgcta agatcgagaa actgctggca tggctggaaa
    13621 cgctgaaagc tgaactgggt attccgaaat ctatccgtga agctggcgtt caggaagcag
    13681 acttcctggc gaacgtggat aaactgtctg aagatgcatt cgat
//
LOCUS       DAJTYF010000106        13544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTYF010000106 DAJTYF010000000
VERSION     DAJTYF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.106"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(1..892)
                     /locus_tag="OO590_003174"
                     /pseudo
     CDS             complement(1..892)
                     /locus_tag="OO590_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1133..1933
                     /locus_tag="OO590_003175"
     CDS             1133..1933
                     /locus_tag="OO590_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6638449.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2011..2781
                     /locus_tag="OO590_003176"
     CDS             2011..2781
                     /locus_tag="OO590_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6638450.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2829..4187)
                     /gene="mltD"
                     /locus_tag="OO590_003177"
     CDS             complement(2829..4187)
                     /gene="mltD"
                     /locus_tag="OO590_003177"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6638451.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4259..5014)
                     /gene="gloB"
                     /locus_tag="OO590_003178"
     CDS             complement(4259..5014)
                     /gene="gloB"
                     /locus_tag="OO590_003178"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6638452.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5048..5770
                     /locus_tag="OO590_003179"
     CDS             5048..5770
                     /locus_tag="OO590_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6638453.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5767..6234)
                     /gene="rnhA"
                     /locus_tag="OO590_003180"
     CDS             complement(5767..6234)
                     /gene="rnhA"
                     /locus_tag="OO590_003180"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6638454.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6299..7030
                     /gene="dnaQ"
                     /locus_tag="OO590_003181"
     CDS             6299..7030
                     /gene="dnaQ"
                     /locus_tag="OO590_003181"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6638455.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7163..7239
                     /locus_tag="OO590_003182"
     tRNA            7163..7239
                     /locus_tag="OO590_003182"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7197..7199,aa:Asp,seq:gtc)
     gene            complement(7370..8416)
                     /locus_tag="OO590_003183"
     CDS             complement(7370..8416)
                     /locus_tag="OO590_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR6638456.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8427..9422)
                     /gene="tssG"
                     /locus_tag="OO590_003184"
                     /pseudo
     CDS             complement(8427..9422)
                     /gene="tssG"
                     /locus_tag="OO590_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9419..11302)
                     /gene="tssF"
                     /locus_tag="OO590_003185"
     CDS             complement(9419..11302)
                     /gene="tssF"
                     /locus_tag="OO590_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6638457.1"
                     /translation="MDPRLLGYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRPLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11318..11812)
                     /gene="tssE"
                     /locus_tag="OO590_003186"
     CDS             complement(11318..11812)
                     /gene="tssE"
                     /locus_tag="OO590_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6638458.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11809..12639)
                     /locus_tag="OO590_003187"
     CDS             complement(11809..12639)
                     /locus_tag="OO590_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HCR6638459.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12626..13540)
                     /gene="tagK"
                     /locus_tag="OO590_003188"
                     /pseudo
     CDS             complement(12626..13540)
                     /gene="tagK"
                     /locus_tag="OO590_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3102 a         3521 c         3617 g         3304 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattatcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aaaattgttc cgctgcccgg ctaaagctgc
      841 cgctttcgac gaccgaaaca aaaatggcga gttcttccga cgtggctttc atttttgctc
      901 ctgttgcaaa atagatgaga tattttgaat ttatttgtca ttaaaccatc aggatgcgtg
      961 atatgtcatc cgatttaatg ttctcaataa tgagcaaatt tctgaccggt gtaagcactt
     1021 gcttacataa caatatacaa ttgctcgttg aaagagtgag ctaaaatccc tataacagta
     1081 gaaccctccc gagtgcggaa gggttgacgt aatagaggtt tcaaagtcaa aagtgcgaaa
     1141 aaacacctat gccatgcgct atgttgccgg acaacctgcg gaaaggatct taccgccggg
     1201 gtcttttgcg agcatcggcc aggcattacc acctggagaa ccgttaagta ccgaagagcg
     1261 tattcggatc ctggtgtgga acatatacaa acagcaacgc gctgaatggt tgtcggtatt
     1321 aaagaactac ggcaaagatg cacatctggt gttattgcag gaagcgcaga caacgccaga
     1381 gttagtacag tttgcgaccg ctaactatct tgccgccgat caggtgcccg ctttcgtgct
     1441 gccacaacat ccttccggtg taatgaccct ttcggcggcg catccagtgt attgctgccc
     1501 gttacgtgaa cgagaaccca ttttgcgtct ggcgaagtcg gcactggtga cggtctatcc
     1561 attacctgac acccgcctgt tgatggtggt taatatacac gccgtcaact tcagtctggg
     1621 cgtggatgtc tatagtaagc agttacttcc tattggtgat cagatagctc accacagcgg
     1681 cccggtcatt atggcgggag atttcaatgc ctggagccgt agaaggatga acgcgttata
     1741 tcgctttgcg cgggaaatgt cgctgcgcca ggtgcgtttt accgatgatc agcgccgtcg
     1801 ggcgtttggc cgcccgctcg attttgtttt ctaccgtggt ctgaacgtca gcgaagcttc
     1861 tgtattggtt acgcgcgctt ccgatcacaa tccgctactc gttgaattca gtcccggcaa
     1921 gcctgataaa taaggtatgt caggtctgcc acagggcaga ccaacgtttg gcgctgtcct
     1981 tttatactca aacaacaaaa ggacagcgca atgacaacac aatcccacca tgaccacgta
     2041 gaaaagcagt ttagctctca agcctgcgaa tatctgacca gtaccgtaca tgcatccgga
     2101 cgagatttac agcgcctggc ggtgcgtctg gctgattatc ctggtgcaag tgtacttgat
     2161 atgggctgcg gagcagggca tgccagcttt gttgctgcgc aaaacgtgag cgcggtggtg
     2221 gcgtatgact tatctgccca aatgctggat gtcgtggcac aagctgccga agcccggcaa
     2281 ctgaaaaata tcaccacccg ccagggatat gctgaaagtc tgccatttgc cgataacgca
     2341 tttgatattg ttatcagccg ttattctgcc catcactggc atgatgttgg tgcagcactg
     2401 cgtgaagtga accggatatt gaaacctggc ggtaggatga ttgtgatgga cgtaatgtct
     2461 ccgggtcatc cagtgcgcga catctggtta cagacggtag aagcattacg cgatacctct
     2521 cacgtacgaa actacgccag cggtgagtgg ttgaggttaa tcaatgaagc caatctgata
     2581 gttgataatt taattacaga taagttaccg ctggaatttt gttcatgggt cgcgagaatg
     2641 cgtacgccag aagcgttagt agacgctatt cgcatttacc aacagagcgc atcgacagag
     2701 gtgagaacgt attttgcctt gcagaatgac ggctctttca ccagtgatat catcatggta
     2761 gaagcacata aagcggcata aataaaaaag gcaccggggg aatcggtacc tttttattat
     2821 ctggtttgtc aggaatctgg catgttgttg tttttcacaa acaacgtcag cttgtcgcct
     2881 ggttgcagat tcgcagtgtc gctgttccag cgcatcacat ctttgatgtt cacgccgtgg
     2941 cgtttagcaa tgcttgaaag cgaatcgcct ttgcgcacac gatacgtaat gctatcgctg
     3001 ttgtttgcca gtcgctgtgc gctactgcct gcaccaatcg tcaaactttg gcctggcttc
     3061 agcttagatc cgcgcagttt gttccactgc tgcaaatctt tggtgcttac gccgagacgt
     3121 gaagcgatgc ttgaaagcgt gtcgccagag cgtacggtgt aaacacggct gttaagcggc
     3181 gtattgtcgg caaccagcgt cgactgtaca gcagcaattt cgcctgaagc gagagattca
     3241 cgcagttgat ctgcatgctt ctttggcacc atcacgtact gcggcccact tgcgcccagc
     3301 gtggagcctt tcacgccagc gttgaatgtc ttcagcttgc tgacggaaat ccccgccata
     3361 tctgcaacct tcgccatttc aaccgggcta ctcaggtgca cacgcgccag agcacggctt
     3421 tcatcggtcg ttggcagacg tacgccataa cgcttgctgt ttttgagaat atcactcaat
     3481 gccagcattt taggcacgta ctgcttcgtt tcctgcggca acggtaacga ccagaagtcc
     3541 gtggatttcc cacgcgcttt gttcgtttta attgccttca tgacccgacc ttcgccgctg
     3601 ttataagccg ctacggtcag aagccagtcg ccgtcaaaca ttttgttcag acgctgcatc
     3661 atattcagcg cggcagttgt tgaagcaaca acatcgcgac gcgcgtcata attgcgggtc
     3721 tgtttcaaac cataattgcg ccccgtgctc ggaatgatct gccagatgcc tgcggcattg
     3781 gcgccagacg ttgcgtgagg atcaaaagcg ctctccacta tgggtagtag taccagttcc
     3841 ataggcatgt tacgtttttt aacttgccct gctatccagt acatatacgg ctctgcccgt
     3901 aaagttacat cgtggagata gctcttattg cgtaaatatt tctgtttctg ttcgcgaatc
     3961 cggtcatttt ccggaattcc catctttagc tcgtcgccaa tgaaagccca caagtcacca
     4021 tctggcgcga tagacgtccc atcgtccatc catcgtgcct gacttgtaaa ctttgctgct
     4081 tccccttgac cagctgcaga aaggctctgt gcgtgctgtt gaacgttgcc ggtactctgg
     4141 caacccacga gcaggacaga ggcgagtaat atcgcttttg ccttcatgtg tgtgtcaata
     4201 gttgcttaaa agacgaccga tcataacggc gaacggagcc gatgacaaga aagttttatc
     4261 agaacctatc tttctttgac cttaaccatg caaaacgctc ttcaggttgt tgcaataatg
     4321 tttcttcatt aattacatta attaaatcaa tatcttccgt tcttaaaaaa acattaattt
     4381 gccgctcatt tttcagaatt acgggtagtg ttatttgatt ttttgcccgt aactccttaa
     4441 ctttacgata ataatcattt atggacaaat cgtgcggaaa aatactcaaa gcaaacttca
     4501 tatttgataa cgtatattca tgagcacaac ataccaatgt atcgtcaggt aacgcactta
     4561 gctttttaag tgattgatac atttgtaaag ctgtcccttc aaacaaccga ccacacccac
     4621 cagaaaacag cgtgtcgccg caaaatagat aaggtttact gaagtaacag atatgtccta
     4681 aagtgtgacc cggtgtggta attacactaa attcatgccc taaaacgaag gcagtttcgc
     4741 catctttgac tacctgtgtt gttcccttat cttgtgtctc ttgtggacca tacaccacaa
     4801 tttgcggaaa cttttccacc agttctttta cgccgccaac gtgatcgtgg tgatggtggg
     4861 ttagaaatat ggcctccggt tgccagttat tggcggtaat ggcgtttaat actggctctg
     4921 cgtctccggg atcgacaatc aggcagcgac ctgcctcatc attcaaaacc cagatgtaat
     4981 tgtcatcaaa ggcgggaata ctgttaagat tcatagatta cctctcagtg taaaacggaa
     5041 ggttgtgatg aaaccggcaa gagtccctca aactgtcgtg gctcctgatt gctggggcga
     5101 tttgccctgg ggagagcttt atcgcaaggc gctggagcgc cagctcaacc cgtggttcac
     5161 taaaatgtat ggttttcatc tgcttaagat tggcaattta agcgcagaaa tcaattgcga
     5221 agcgtgcgcg gtttctcatc aagtgaatgt ttctgcgcaa ggaatgcccg tccaggtgca
     5281 ggcggatcca cttcatcttc cttttgccga taaatccgtt gatatttgtc tactggcaca
     5341 taccttgccg tggtgcaccg atccgcatcg tttattacgt gaagccgatc gggtactgat
     5401 tgatgatggc tggctggtga ttagtggctt caaccccatc agtttaatgg gattacgcaa
     5461 acttgtgccg gtattgcgca aaacctcgcc ctataatagc cggatgttta ctctgatgcg
     5521 gcaactggac tggctctctt tgttgaattt tgaagtgctg cacgccagcc gtttccacgt
     5581 tctcccgtgg aacaaacacg gaggaaaact attgaatgcg catattcctg cgcttggttg
     5641 cttacaactt attgttgccc ggaaacggac tattccttta acgctaaatc cgatgaaaca
     5701 gagtaaaaac aagccacgaa ttcgccaggc ggttggagcc acccggcaat gtcgtaaacc
     5761 acaggcttaa acttcgactt ggtagcccgt atcttccagt gtgggattca tcgccgcggc
     5821 acgagccagt tcatcacagc gttcgttttc cgggtgtccg gcatggcctt taacccattc
     5881 ccatttgatt tgatgctgcc ccaatgcagc atcaagacgt tgccagagat cgacattttt
     5941 tactggtttt ttgtctgctg ttttccagcc acgttttttc cagttatgga tccactgggt
     6001 gataccctgg cggacatact ggctgtcggt actcaaaatg acttcgcaat gttcttttaa
     6061 cgcctccagc gcgacaatag cggccatcaa ctccatacgg ttgttggtgg tgcgggtgta
     6121 gcctgcgcta aaggttttct cgcgtccgcg atagcgtaaa atagcgccgt aacccccagg
     6181 tccaggatta cccagacacg aaccatcggt gaaaatttct acctgtttaa gcatctctgg
     6241 tagacttcct gtaattgaat cgaactgtaa aacgacaagt ctgacataaa tgaccgctat
     6301 gagcactgca attacacgcc agatcgttct cgataccgaa accaccggta tgaaccagat
     6361 tggtgcacac tatgaaggcc acaagatcat tgagattggt gctgttgaag tagtgaaccg
     6421 tcgcctgacg ggcaataact tccatgttta tctcaaaccc gatcggctgg tggatccgga
     6481 agcctttggc gtacatggta ttgctgatga atttttgctc gataagccca cgtttgccga
     6541 agtagccgat gagtttatgg actatatccg cggcgcggag ttggtgatcc ataacgcagc
     6601 gttcgatata ggctttatgg actacgagtt ttcgttgctt aagcgcgata ttccgaagac
     6661 caataccttc tgtaaggtca ccgatagcct tgcggtggcg aggaaaatgt ttcccggtaa
     6721 acgcaacagc ctcgatgcgc tatgtgcccg ctacgaaata gataacagta aacgaacgct
     6781 gcacggggca ttactcgatg cccagatcct tgcggaagtt tatctggcga tgaccggtgg
     6841 tcaaacgtcg atggcttttg cgatggaagg agagacacag cagcaacaag gtgaagcaac
     6901 aattcagcgc attgtacgtc aggcaagtaa gttacgcgtt gtttttgcga cagatgaaga
     6961 gctggcagca catgaagccc gtctcgatct ggtgcagaag aaaggcggaa gctgcctctg
     7021 gcgggcataa atacatgtaa aaggcgctaa aaatagcgac ttgggcgatt tttgcagcaa
     7081 acgattcaaa agatgagaaa aatcgttgac gaaggtcgag gcaatccgta atattcgcct
     7141 cgttcccaac ggaacacaac gcggagcggt agttcagtcg gttagaatac ctgcctgtca
     7201 cgcagggggt cgcgggttcg agtcccgtcc gttccgccaa tattcagaaa gcccgaatca
     7261 gtaatgattc gggctttcgt cgttttggtg aagtgagaat ttttcactcc gaagtaaaac
     7321 ggatatgatt taaaaaatgg ccagacatta taaattctgg ctacttctgt tactccagcg
     7381 catcccattt ctccagcagt tctactggaa tttcatttat tacctccgag acgcgttttc
     7441 cgaccaggcg ttctgcctgg cgcagcaaag gaatggtcgg gctgctcggt tcgctgtgct
     7501 caaaccagcg acggatgaca cgcaggcgtt ccagcgcgtc gtttcggtcg cggatctgcc
     7561 ggggttctgc gccggtcatc ggtgatattt cctccgcgat atgcaccggt agcgcaatct
     7621 gctctgcctg catctgttca gtatgaacga tggtggcgtt ttccggctgc tcagcctgtg
     7681 gttgtggcag gatctccggc gtggtgctct gcactgctcc cggtaacagg gccagcagtt
     7741 gcgtcagacg ggagaagtcc ggtgcctggt cattcagcgt ttcacgcgca tggcgttgca
     7801 gccgctcagt ggtctctgct gcctggcgaa aggcatccag cggcagtgtg ccgcgcgctt
     7861 ccaggtctgc cagttgctgg cgtaccgatt ctggtgccag cgcatccgcc gggcgtaatg
     7921 cagatagcga acgctccaca tcccgcacct gaaggcgcat ggcagcatta tttgacagtg
     7981 taatgccacg aatatccgcc attacacctt cgtggtccag caacgccgcc agcgcgttac
     8041 tgcgtgccac cgcagcatct tcggcggtga tatcttcagt cgcgagcagt tgaggatgga
     8101 tggcgtccgg gtacgtggag cacaacgttt ccagcagcgt gagcgcctcc gacagtcccc
     8161 tggcaccggc ctgctggata cggcagcgca gcaggacaac cagaatgcgg atatctttgc
     8221 tgcgcatcag cagacgatgg gcgtcccgct caagctccgc ccagttaatg ttttccggcg
     8281 tactgacaaa atcgccatat tgcgcttctt cacgcggtgc ggcgcgggtg aacagcagca
     8341 ggtattccgg atcgtactcc agatccgggc cgcaggggca ttccgccgaa attgggtcag
     8401 acaggaatat ttccatttaa ttatcctcag tgcgccaggt agtgttctgg ttcgaaaatc
     8461 atgcccgtca ccgggttgtc gtgcatggct ttacccgccc atgcagtcca acccagttgg
     8521 tacggaccac cggtaacagc cggaggggcg gcgtggggct ttagctgtag ctcaatttcc
     8581 cagcagtatt cataaccact aaaggcgcgg accagttctg tcaggacggg caggttattg
     8641 ctgccaggca gaaaacgtcg gtaatcctcc agcgtcagcg ggccgataac cagccggaac
     8701 ttatactgca tgtccggcac ggcctggccg atgagtgcac tattgccaat aacggaagag
     8761 ggggcaggca tccccagccg ggtgatctca tcgtccgcca cggctatcca gtgcaggaca
     8821 tactcctgta tccggaaagg tacaccgaaa tagtgcgcca gcgtggtggc cagaccgtcc
     8881 ggattgcggg actcacgcac cagatgggcc gatgccgcca gacgagcgtg atctggcaac
     8941 gggctgtcag cgctctcccg caaatcctgc ccgctgaggc tggcgacata aaaggcaaag
     9001 cggtcatgtt gcggtttatc cagaccgccc ccagcggact gggcgctgcg ccatgcctgg
     9061 taaaactgcg tcagccagcg gtgatgaaaa agatcagaaa aacgggtcag cgtcggatcg
     9121 cgtcggcttt cagtccggtt acgcgccagt tcggtgtagt gcagcggcag tggaccgttc
     9181 ggcccccaca gcccgaggct gtacagactg atgtgcaggc gcccttcctg ccagcccaca
     9241 ctggcgattt ctcgtggcgc aaaggtcagc gcgggcgttt gtccgaggcg aaatttttcc
     9301 atccgtggtt gctaggtgtt gcccgccgga acggtacaca actgcacatc cacgcggcgc
     9361 atcaggttca gaaaaccgta atgccagggg ggttcctgcg cctgagtgaa atcgttgctc
     9421 atacgcttcc ccgctgtccc gccctgacag gccaggtcat aattttgccg cgctgcatgc
     9481 tgtgcagcgt catctgcgag aaggtattaa tggaaacatg gcgggcgata tagtgttcca
     9541 gcaccaggcc gaacagatac ggactgatgc cggaaaaacc ttcttcatcc accgtcagtt
     9601 cacaactgac gccacgccca tagaccagta gtccggaacc gggcagacgg cgggtgaccg
     9661 gggtcgtttt acaaccaatc aggctgcgca cctggcgcga ttgcggactg tcatgagcag
     9721 gaataaacag gttcagcaga tctcgcaacg cctggccgcc ggtacggtga tccagatcgg
     9781 ccagcggcag ataattaaaa gataactggc ggatcaaccg ccatgccatc tcacgttcag
     9841 ccagcggggg ctgcggcgag cgtggcgggc ggataagccc tacgcccgcc accgggatgg
     9901 ctgcatccac ggttagatcg tcccggccat tgcgggcaat aagacagggc agatcgcggt
     9961 tagtgaccat ggccgtgatg gtgatgtggc gcagattttc cgggtacggc gcttcatact
    10021 ggtcaaccag cgacaggaac acttccgagc ccgtataggg ggtacgggtg ccgtaacggc
    10081 gggcgttttc tgataagcgg cggggctccc ggcgcaggga aaaataacgt ccgtgattgc
    10141 cttcatcatt attgcgggtg tggtagagcg ggcgaaaggc catcttccgt gtggtgtcgg
    10201 tttccagccc ttccacttcc tggaccgaga acacctcata atccagcgga tgggttctgt
    10261 ccaccaccag atgctgttcc gtcgtgctgt gcgtgacatc gatccgggcg gtgacgcggg
    10321 gaaacaggtt gataaccggg gtacagaaca ggctgaactg tgccgcattt gtctggtgga
    10381 tcagccagtc cggcggcagg cggttcagca ggataacaat ctccgccacg ccaccgtcaa
    10441 ttttctgtaa tccggcagac aggcctgtcg gggtgaaaaa atagaagcgt tccggacagg
    10501 caaaatattc atgcagcagg ttatgaccgt gaaacacgtt ccacgccagc ggcagcagcc
    10561 cctgatccgg ctccaggcct tcgtacataa cgggctgttg cagattcaca tccagcgcgc
    10621 cgtcaaaatg ccccggaatg ccagccaggg tcgcgatggc gctggtatgt agaagttcta
    10681 acaggtgtga ggccgtacgc tcctcgccgc agagataaaa cggcagcctg tccagtccgg
    10741 caagctgact gaacgttagt tcaccaaagg tgcgcagggt aatacgcagc gccccggcga
    10801 cgtgaatgtt ggccggcagg tagcggtgca gggcaggcat atccggtggc gcggcggtca
    10861 gtcgcgcctc ttcgatggcg agcggccaca gcgttacatc ctggctgctg cgaaactggc
    10921 aggcagtgct ttcgccttcc ggaatggcgg agaaaaaggc tgtgtcgcgc gggacggtaa
    10981 atcctttcgc cagatcgcct tcttccgtat cgggatgcaa ctgcgccacg ctcattgagg
    11041 gcgtgggtgt gacgtaattg gggctgacca cttccagcgg gcgctgggtg aaacggggga
    11101 attcagcgtc aatttttagc tgcgtgcggg cggtcaggaa gctgaaggcc tcgaccattc
    11161 gctctacata cggatcggca atatccgttc cctgcatgcc cagccgggcg gcgactttgg
    11221 gatgacgggc ggcaaattcg gctcccgttt cccgcagata gctcagttcg cggttgtagt
    11281 atcccagtag ccgtggatcc atcggtctct ctctgaatta aaaaaacgta atgcgactct
    11341 gctccatatc cagcgcgcta cgtacccgga atgcagtcgg atacggctgc gtgaggatct
    11401 cgccgcgaat ttcaaactgt aatgtgttat ggccactctg gcggcgctca tcctgaagtg
    11461 ggatgatttg cagggtggac gctttcagcc ggggttcaaa ccgggtgatg gttcgcaaaa
    11521 tggccctgcg gatatcgtcc cacttgtgct catacagaaa gctgcccgcc agtggcggca
    11581 ggccgtagtt aagtacggac gctgccgcgt acggataacg tgcagcatca atatcatctt
    11641 catggctgac ggtgttgagc agaaatgaca aatcccggcg gatgatctcc ttaatctgaa
    11701 ccgggctgac gctgataccg tggtcgcgtt tctggtttgg ggcgtcgtca cacaggcggt
    11761 caaacagcgt gggcagcagg tgattgcctg gacgctggcg ggaagcgctc acgcgtcgct
    11821 tccttcagcg gtgttaaagg tacaggtgga caaatccagc aggctgatat cgccgtgacc
    11881 ggtgagccac accctctgac ccagcgcctg taccagtgtc tctcccggac cgtcctgcca
    11941 gaccgtttcc cggcacagac gcagggagtc ggcagccttt tcagagccgc tgtagcgggg
    12001 aaacagccag gcactgtgtg tatcgccatt gttcagggtg acgttgacgg gtttccagat
    12061 caaatcgatc agatgcgcag gccgcggcga tttcagcgaa cagatttgtg aaaacggcag
    12121 ccagatatat gctccgcccg cgatcagttc gagtaccgga ccggtacggg agtcgctgtc
    12181 gctaatccag tcaaatgcgc cgccattcca ctgcccggaa gtgtctgtta tggcctcaag
    12241 cgcgcggctg cggtgagcat cagcttcctg cgcttcacca cgggcgttac aggcgagggt
    12301 ggtcagcaga tcctccatcc aggcgggcgg cgggagaata acaccgggcc gctgttcgcc
    12361 ctgaaagcag gcgtggcggt atatctcaca gcggatcagc tcgccaaata cctgcgcttc
    12421 gcgagtataa tttgcgtcca tccgggcgca tagctggatc tgttgcagtg cccgcgacca
    12481 gtcgcctgcc acgcacaata actgaaacag gctgtggcgg cgggaggcct gcgctgggtg
    12541 catcctgacc tgttgctctg ccatctgaat gccctccacc acggaataat cccgcagcag
    12601 cgccgacaag gtaatcggta gctcgttatt gtttgtcatg atatatctct ccatgttgtg
    12661 ccgggtttat ccggtaatgg ttgtcaatgc caatgatgcg atgctctttc cgtgtcagat
    12721 ctggcagtac ggtgttatgc gctgtttccg gggcgtattc cggggaaagt aaatgcagga
    12781 tatcaggggg agtcttttcg acaggccaga tagtttcatc ttcgccagtg gcatccagta
    12841 cattgagaat agcgtcaatg cttaacccac cagtgaccat atcctgcaat gtctcggctt
    12901 cggtgaccag gtcacgcaat gaatccagat cctgcgagga gggctcttcg ttaaaaggaa
    12961 aaggatcagc atggcgatta agatcttgtt cgggagataa caacgcctcg cggtattcct
    13021 gataaagtcg ggtcagggca tttccttttt ccgcaccttc tccggtggag gaggctccat
    13081 gaacaggaat gatatcgaat ggattttgtg atgtcagatg ctgctgcctg aaccaatcca
    13141 gatccagata ttcggttatt gcttccgacg tgatggcagt ctgcgatatc gtttttactg
    13201 tgctcaggtc cggagatgat ggattgccat gtacaaggca ctatgaagat aacccccatt
    13261 ctatcaggtc gccttcattc agacgcatac ggtgatgagg ttccacaatc tggtcgttta
    13321 ccgtacagca atattcggta gactgattga taatccacca tgcttcttcg tggcgggtca
    13381 gagtgagggc aatctttaca tcatcattca ccttaaaaac aggaatccag ggagaggtaa
    13441 cggtgaagat aatttgtgtt ccttcagtcg cgtaaattgt gttatcggcg atcccgtgcg
    13501 cctgtatttt ttggagtccc cacgtaatat ttggtttcat tgtg
//
LOCUS       DAJTYF010000107        13434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTYF010000107 DAJTYF010000000
VERSION     DAJTYF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.107"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..343
                     /locus_tag="OO590_003189"
     CDS             <1..343
                     /locus_tag="OO590_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6638460.1"
                     /translation="ECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQPDN
                     LLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLR
                     ETIKNLYASMA"
     gene            500..1249
                     /locus_tag="OO590_003190"
     CDS             500..1249
                     /locus_tag="OO590_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6638461.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1271..1837
                     /gene="yieF"
                     /locus_tag="OO590_003191"
                     /gene_synonym="chrR"
     CDS             1271..1837
                     /gene="yieF"
                     /locus_tag="OO590_003191"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6638462.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1891..3228)
                     /gene="adeP"
                     /locus_tag="OO590_003192"
     CDS             complement(1891..3228)
                     /gene="adeP"
                     /locus_tag="OO590_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6638463.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3393..4058
                     /gene="yieH"
                     /locus_tag="OO590_003193"
     CDS             3393..4058
                     /gene="yieH"
                     /locus_tag="OO590_003193"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6638464.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4125..4598
                     /locus_tag="OO590_003194"
     CDS             4125..4598
                     /locus_tag="OO590_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR6638465.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4647..5234
                     /locus_tag="OO590_003195"
     CDS             4647..5234
                     /locus_tag="OO590_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR6638466.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5296..6018)
                     /locus_tag="OO590_003196"
     CDS             complement(5296..6018)
                     /locus_tag="OO590_003196"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6638467.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPILGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNMLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6020..7195)
                     /locus_tag="OO590_003197"
     CDS             complement(6020..7195)
                     /locus_tag="OO590_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR6638468.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYLLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7222..8838)
                     /gene="bglH"
                     /locus_tag="OO590_003198"
     CDS             complement(7222..8838)
                     /gene="bglH"
                     /locus_tag="OO590_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR6638469.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8924..10321)
                     /gene="bglB"
                     /locus_tag="OO590_003199"
     CDS             complement(8924..10321)
                     /gene="bglB"
                     /locus_tag="OO590_003199"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6638470.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10337..12214)
                     /gene="bglF"
                     /locus_tag="OO590_003200"
     CDS             complement(10337..12214)
                     /gene="bglF"
                     /locus_tag="OO590_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6638471.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(12348..13184)
                     /gene="bglG"
                     /locus_tag="OO590_003201"
     CDS             complement(12348..13184)
                     /gene="bglG"
                     /locus_tag="OO590_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6638472.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         3547 a         3373 c         3078 g         3436 t
ORIGIN      
        1 ggagtgtccg ttaaactcag gctacctcag ggcgctggac aatgtgttac aggagctggg
       61 gcgcgaacgc acgattgcta tgagcctgcc ggaattcgag cagtcactgt ttatggcagc
      121 gcaacccgac aatctgctac tggcgaccgc gccgcgctac tgtcagtact acaatcaact
      181 ccatcaactg ccgttggttg ctcttcctct cccgtttgac gaaagccagc aaaaaaagct
      241 ggaagttcct tttaccctgc tgtggcataa acggaacagc cataatccga agatcgtctg
      301 gttacgggaa accattaaaa acctttacgc gtcgatggca taaccgaatc gtatgaaacg
      361 cgacccaatt tcacaataaa atgtaaaaaa gttgtaataa gcttgtctga atcgaacttt
      421 tagccgcttt agtctgtcca tcattccagt aaatgattac tcttgtattc ataatggacc
      481 attaagcatg gagcgaaaaa tggcgactca ctttgcccga gggattttaa cggaaggaca
      541 tctgatttct gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca ttccccctca
      601 tcgtcaaagc cgttttctgg cgtccagagg tttactcgca gaactgatgt tcatgctgta
      661 tggcattggc gaattgccgg aaatcgtcac cctgccgaaa ggtaaaccgg ttttcagtga
      721 taaaaatttg ccttcgtttt ctatttccta tgccgggaat atggttggcg tggcgttaac
      781 aaccgaaggt gaatgtggcc tcgatatgga actacagcgt gcgacgcgcg ggtttcatag
      841 cccacacgcg cccgataacc acaccttttc cagcaatgaa tcgctatgga tcagtaaaca
      901 aaacgatcct aacgaagcgc gggcgcagct cattacgctg cgccgaagcg tgctaaaact
      961 aaccggtgat gttttgaatg acgatccgcg cgatctgcag ctgctgccca ttgccggacg
     1021 cctgaaatgt gctcatgtaa atcatgtaga agcgttatgc gacgcggagg acgtgctggt
     1081 gtggtccgtg gcggtcacgc ccgcgattga aaagctcagt gtctgggagt tagatggcaa
     1141 acacagctgg aaaagcctgc cggatattca cagccgcgcc aacaatccta ccagccggat
     1201 gatgcgtttt gcccagctct ctaccgtgaa gtctttttcg ccaaattgat agacaaccac
     1261 aggagtcatt atgtctgaaa aattgcaggt ggttacgtta ctggggagcc tgcgcaaagg
     1321 ctcatttaat ggcatggttg cacgtaccct gccgaaaatt gctccggcga gcatggaagt
     1381 caatgcgtta ccttccattg ccgacattcc cctgtatgac gctgacgtac agcaggaaga
     1441 aggttttcca gcaacggttg aagctctggc ggaacagatc cgtcaggctg acggtgtggt
     1501 gatcgtcacg ccggaatata actactcggt accgggtggg ctgaaaaatg ccatcgactg
     1561 gctttcccgc ctgccggatc aaccgctggc cggtaaaccg gtgttgattc agaccagctc
     1621 aatgggcgtg attggcggcg cgcgctgtca gtatcacctg cgccagattc tggttttcct
     1681 cgatgcaatg gtgatgaaca agccggaatt tatgggcggc gtgattcaga acaaagttga
     1741 tccgcaaacc ggagaagtga ttgatcaggg tacgctggac cacctgaccg ggcaattgac
     1801 cgcatttggt gagtttattc agcgagttaa gatctaaata aaaaacccgc cagtaatcat
     1861 gcatggcggg tttttaatgc gctatcgatt ttaatgagcg tcgataaata caatcttcag
     1921 gataaacagc agcgcaacga tgattacgca cgggctaagg tcacgcagac gcccggtacc
     1981 aatcttcatc acgcagtagg agataaagcc cagcgcaata ccttcggtaa tcgaaaagct
     2041 gaacggcatc atcacggcgg taataaacgc cggaacagat tcagtaagat cctgccagtt
     2101 cacgcgagca agacttgagg tcatcaacac gccaacgtag atcaacgcgc cagctgcagc
     2161 gtagcctggc accattcccg ccagcggcga cagaaagata accagcagga acagcagacc
     2221 aacaaccact gccgtcagac cggtacgacc gccaaccgat acgccggaag aggactcaat
     2281 ataagccgta acggaagaag taccgataaa cgaaccggtc acggaagaga tactgtcgac
     2341 atacagcgcc tgcttcatgc gcgggaattt ccccttctca tccgccagac ctgctttatc
     2401 ggtcacgcca atcagcgtac cggaggagtc aaacaagttg accaacatga aagagaaaat
     2461 cacccctgcc agcccgaggt taaacgaccc ggctaaatct acatgaccca caactgtcat
     2521 tacgctcggc ggcgcagaaa cgatgccatt gtagtgcaca tcacccagca tccagcccag
     2581 cagcgtcgtc accacgatag aaaccagcac cgctgcgtga atgttgcgcg aggccagaat
     2641 agcaatgatg aaaaagccga ggatacccag aagtacgctg tgagaagtca gattaccgat
     2701 gctcaccagc gtttccgagt tagcgacaat cacacctgcg tttttcagcc ccatcatgcc
     2761 gataaacagg ccgataccgc tggtaatacc cacacgcaga cttaccggaa tgttagcaat
     2821 catccagtaa cgaacgcgga aaatggtcaa aagaagcagt ccgacagcgc cccagaagat
     2881 tgcgcccata ccgatctgcc acggcagccc cattgcctgg acaaccacaa aagcgaaaaa
     2941 ggcgttaagc cccatcgcag gagccagcgc caccggaagg tttgcaaaca atcccatcat
     3001 aatgctaccg aaagcagcaa tcagacaggt tgttacgaag acggcactgg tgtccatgcc
     3061 agcaacgccc agaatttgtg ggttaacaaa tacgatgtag accatcgtca ggaaggtggt
     3121 gaacccggca atcacttcag ttcgtgccgt agtaccatgt tcgcgcagtt taaaaacgcg
     3181 ttccagcatc ctctgaccag aagtctgggt ggtatgttga tgactcatta tctatttccg
     3241 aacaaggagg gaaaatccgt cgctatcgta taccaaaatg cgacaatagg cgcgtttgtg
     3301 agagactttt ttattggatt tacttatacg gcaacgattg cgttgcgcaa atcggctcta
     3361 cgaaaacgtt aaactgatta aaaaggaaag gcatgtcccg gatagaagcg gtatttttcg
     3421 actgcgacgg tacgctggtc gacagtgaag tcatttgctc ccgcgcatat gtaacgatgt
     3481 ttcaggaatt tggtattacg ctcgatcctg aagaggtatt caaacgtttc aaaggtgtaa
     3541 aactgtacga aattatcgat attgtttccc ttgaacatgg tgttacgtta gcgaaaacag
     3601 aagctgaaca cgtttaccgt gcagaagtcg ctcggctgtt cgattcagaa ctggaagcca
     3661 tcgaaggggc tggagcgctc ctgtcagcga tcactgcgcc aatgtgtgtg gtatctaacg
     3721 gcccaaataa caaaatgcag cattctatgg gcaagctgaa tatgttgcac tacttcccgg
     3781 ataaactgtt cagcggctac gatattcagc gctggaagcc agacccggcg ttaatgttcc
     3841 atgcggcaaa agcgatgaat gtaaatgtag aaaactgcat tctggttgat gactcagttg
     3901 ccggtgcgca atctggtatc gacgcaggta tggaagtgtt ctacttctgc gccgacccgc
     3961 acaataagcc gatcgttcac ccgaaagtca ccacctttac ccatctttcg cagttaccgg
     4021 aactgtggaa agcgcgtggt tgggatatta cggcatagtt cttcacactc ccttcactta
     4081 ccccgcttaa attggcgctc aaaggtaagt aaagggagtt tgatatgtct gtttcacgtc
     4141 gggtaataca tcacggactt tattttgcgg ttttaggacc gttaattggt gttctgtttc
     4201 ttgtcctcta catattcttc gcaaaagaac cgctggttct tttggtaata atacaggtac
     4261 ttccattgtt tctcttattg tcgattacta cgggagctat tcctgcattg ttaaccggcg
     4321 tgatggtcgc ctgtctgccg gaaaagatcg ggtcacagaa aaattatcgt tgtctggctg
     4381 gtggcatagg tggcgtcgtt atcaccgaga tctattgtgc agttattgta catattaagg
     4441 gcatggcttc ctcggagttg tttgaaaaca ttctttctgg tgacagtctc gttgtccgca
     4501 tcattcctgc attgctggca ggtgtggtga tgagcagaat cattacccgt ctgcccggat
     4561 tggatatttc atgtcctgaa acagactctt taagttaagc gggatacttt atctttgggc
     4621 tactcaaaag cagacaggat gttactatga ctcaaaatat caggccgtta ccccaattca
     4681 aatatcatcc caagccactg gaaacaggcg catttgaaca ggataaaacc gtagagtgcg
     4741 attgctgtga acaacagacg tcagtttatt actcgggtcc cttttattgc gttgatgaag
     4801 ttgaacatct ctgtccgttg tgtattgcgg acggttctgc tgctgaaaag tttgcaggta
     4861 gttttcagga tgatgccagc atagaaggtg ttgaatttga gtatgatgaa gaggacgaat
     4921 ttgccggtat taagaacaca tatcctgatg aaatgctgaa agagttggtt gaacgcacgc
     4981 caggttatca tggatggcaa caggaattct ggcttgcgca ttgtggcgat ttctgtgctt
     5041 ttatcggcta tgtgggctgg aatgatataa aagatcgcct cgatgaattt gccaaccttg
     5101 aagaagattg tgagaatttc ggtattagaa attctgatct agctaaatgc ctgcaaaagg
     5161 gtggtgattg tcagggttat ctcttccgct gtctccactg cggcaagctg agactgtggg
     5221 gtgatttttc gtagttattt aaataatgag aacaggccgg agcgtaattc atacatccgg
     5281 ccttatttct tcagcttaac cgaacgctaa ttctgcggca gcggctttat cagcgattac
     5341 catcagcgaa ggatgcagct gcaacacgga agcaggaaca tcttcggtaa ccggaccttg
     5401 cagcatattt ttcagtgcct gcgctttacc tgcgccactg acaattatca gcaagttttt
     5461 cgccgccatg atgcttttcg gccccatcgt cacgtagctg tcaggcacta acgaaaagtc
     5521 gccgcccaac tcaccatgcg ccacaatatc aaccatctct cccaggatcg ggaactccac
     5581 ggtttgctca tggaaatggg tcgtattcgg caaattgcca caaaaatgac catctgcacc
     5641 taatcccagc accaccaaat ccaggccgcc ttcacgcgcc agtttctgat catgctcgcg
     5701 gtagttatca atagtgagct tctggatatt ctcttctttg atccccgcag gggtgaaaaa
     5761 cagattacgc agattggtaa tcgttacgcc ttctccctct ttgccgcgaa agggaatttc
     5821 atcgaaatta tagaaatagc agttatcgta ccaaggctta cctttaacca gggtggtgag
     5881 gtattcatac atgcctttgg gcgtgctacc ggcggtaatt gccaggttaa cacgacgcgt
     5941 cttcgacata taaccaagca gatggtgtgc cgcgacacgg ctcatttcct ggtaatcttc
     6001 ggtaatgatt aatttcatct taatgcctta ttttaaattt ctgtacgaag gctgcatagg
     6061 caggccgcca gacatccatt tcatggttca ggccgggata ttcctggtaa tcaaacttaa
     6121 tttttttctg ctcaagctca gttttcagcc cggcgatatc cttgccggtt acgacatctt
     6181 tatcaccaac gaccacagta aaattacgta gttgctggtt aatagctgcc ggatcgttca
     6241 gccgggccgc gacaccttca tccggtacgg ttgtcgtggt aacaccactg aatgtggcca
     6301 gccagccaaa gctttccaga tgattcattc cggaaaccag cgcctggtac ccgccttgtg
     6361 aaagccctgc cagcgcgcga ccatcggcat ctttacggac actaaaacgc ttgctaatca
     6421 gcgggataat atcgttcatc agttcgcgat ctgccgcttt agcattcagc agataaaaga
     6481 ctttacgtct ttcctgaggc acgaaatctt cgggaataat gcccttcgca tcggtttcgg
     6541 tatccgggat caccaccagc atcggtttaa ttttcccttc agccagcagg ttatccatga
     6601 tttgcgggat acgcccctga tcgatagcgg aacgtccagt atcaccaaaa ccgtgataga
     6661 aatagagcac tggcaaaggc tcgcccatgc cggtgtatcc tggcggggtc cagacataca
     6721 tctgacgttc agattgcaat gcgttggagt ggtaagttat ggcgatcaaa tcaccatgcg
     6781 caacagaacg cgtatccaga taactgccta gcaccagaat catactggag ttaacctggc
     6841 gctgaggctt agtcattgcg gtgcctgtat caatgctgcg tacaccatca acattgaaaa
     6901 aatactcgta caggttgcct ttcaggatag gtgtgcgcca cgaccagact cctgcttcgt
     6961 ctttggtcat cgggtgaata ttgtccggaa ccggaacacc caccactaca gagacatttt
     7021 ttgccccagg ggcaaagtag cgaaaggtca cgctgttatc ggcattgacc tgagtcacat
     7081 actgtttcac cgggatagtg ggtgccgggc tggctggcat atccgctgct atggcccact
     7141 gagcagaaat accgctggca atagccagcg ttaaagcagc aatttttata ttcatcgtaa
     7201 tattcctttc attaagttcc attaccacca gatttcagcc tgggcaccga cagcaaactg
     7261 gtcgtcttta ttgtcttcga aggtgaagcc atccagttcg ttttccaggg ctttgatata
     7321 cgtggcgtag aaacgaattt ccagacgcga ggtcaacata ctggtattga ctttaaaggt
     7381 atggaagagc gtggttttat aaccagactc attaaattta ttactattcg catctttgtt
     7441 ttgctgggtg aaatagccca gttcaacacc tgtctggtta tattggtccc aaatataggc
     7501 cgggcgaaca accgcacgaa tagattcgaa atcagagtgt gcgcctgttt cgtagctata
     7561 aatatcgtta ccgaaggagt aaacaatcgc gttagccaca ataaaatgat cgccgatata
     7621 ggcttcgccc tgcgaggtca gacgaactgc tgttccgccg gtgtgatcac cataataacg
     7681 accattaaag gtggtaaatg gacttgcgcc agcataacgg ccaaagttcc tggcgataga
     7741 gttattcgca accaggaagg agaattcgtt gaagccacct ttatcaaatt tctgcgttaa
     7801 agatgtgccg aacatccagg tatctttcca gtcataatac ccgttattat cctgattatc
     7861 tttttccgat gcgctttcgt ttgccgtgac ataacgacca ctcaccatta aggtcgcttt
     7921 atcccataac gggatatctt tatagcgtaa atcaatagta tgggtattaa tctgctgttt
     7981 gttttgcagg ctgcgatcgt aatcatcgac atcttcgcga accagcgcga tatcaatttt
     8041 acccggaccg actttccagt tttccagacc tacacccgct gcggcatcag tacgctgcgt
     8101 tttccagtcc agcatctgga tttcaatttt cggcgcaccg tgtttaccca cccagaaatc
     8161 agcctctggc gcaaagggca ggaaaccttt ggtggtaacg tacatatcgg agaactgcat
     8221 aaagttctcg ccaccggcgt tatcgccaaa ccagccggta gagtactgct gaccaacgtt
     8281 accatccatc atcacaacgg catcaacccg tttgccgttt tcgttgtaga cacgttgttt
     8341 taactgcaaa tcaaaccagc cggagtattc gttaccaaag cggcccagag aaccaatcgc
     8401 ccatgattta ggtgaaccat gagaggcggt cccccaacca gaacggtaat agccgttgta
     8461 actaaagcca atttcatctt tcacaaactt actgaaatct ttcagagtca tggaagaata
     8521 aatcgagcct gtctcactgg catttttctc ttcatttttg accagtacag catcaggttt
     8581 tgcggcacta ctggtcggga acggattggc ggcataccct tgatcattcg tacttacgct
     8641 acgattcacc gtcgctggcg tatatttttt cttctcttca tctttatact ttttgagttc
     8701 gctttgcgtt tctcttaacg ctttttctaa taactcaagg cgttgttcca ccgttaatga
     8761 ttcagccaat gattgtgcag aaaaggctaa cggtgccatt aataagatgg cagaggtaat
     8821 aagatttcgt ctaaacataa tatcccttta tggtgcaaag aaagaattaa cgtatcgcat
     8881 ccagactgtt ctgaatgcga cgataattaa ggtgctttat tggttatttt tttaatgaca
     8941 gcccccgcgt tttgatcact tctgcatacc atccgaagct tttcttacgc gttcttgtga
     9001 ggcttccttc gccattatca tcacgatcca cataaataaa gccgtagcgc ttagacattt
     9061 gtgaatgaga tgcactgact aaatcaattg gcccccaact ggtgtacccc ataatatcca
     9121 caccatcggc aatcgcttca tttacctgta ccaggtgatc gtttaaatag gcaattcgat
     9181 aatcgtcctg tatcgaacca tccgcttcaa cgctgtcttt tacgcctaat ccattctcga
     9241 caataaataa cggtttttga taacgatccc aaagcgtatt taacagaacc cgtaatccaa
     9301 ccggatcaat ttgccacccc ctctctgaac ttttcagatg cggattgggg atcatattca
     9361 gtatgttgcc ctgcgcattt ttattaatgc tttcgtcgtg ggaaacacaa ccagtcatgt
     9421 aataactaaa agagatgaaa tctacagtat gttttaaatc ttctgcgtca ctttcagtca
     9481 tctcaatggt gatattgtgg tcgcggaaga aacgctgcat atagccggga tactggccac
     9541 gcgcctgaac atcaccaaag aacatccagc gccggttctc ttccatggcc tgcaacatat
     9601 cctgtggctg gcaggtgagg gggtaaacca gcccaccgag aagcatattg ccgattttcg
     9661 cttcggggat caggctatga taggctttaa ctgcccgcgc actggcaacc agttgatgat
     9721 ggatcgcctg ataaacttcc gcctcgccac tctcttctgc cagccccacg cccgtgaatg
     9781 gcgcgtgtaa cgacatattg atttcattaa acgtcagcca taacgctact ttatgttggt
     9841 agcgggtaaa gaccgtgcgg gcgtaatgct cgaagtgatc gatgaccgcc cgattagccc
     9901 aaccgccgta gtttttcacc agcccatata gcatttcgta atgggataac gttaccagcg
     9961 gcttgatccc cgcctgcgcc atttcatcaa acagccgatc gtaaaacgct aaccccgctt
    10021 cattcggttc ggcttcgtcg ccctgaggga aaattcgcgc ccaggcaatg gaaatacgca
    10081 gacaggtgaa gcccatctcg gcaaataacg cgatatcttc cgggtaacgg tgataaaaat
    10141 cgatggcgac atctttgata ttctctttcc ccaggatgcg cggttccatt tttcccatta
    10201 cgccatgagg ctgtaaatct gaggtcgtga tccctttgcc atcttccagc caggcacctt
    10261 ccacctgatt ggcagctgtt gcgccacccc aaagaaatgt ttctggaaat gctttcgtaa
    10321 ttaactcctt ttatcgttag cgaatgatgg ataacagcgg ttcacctgcg cttatctgcg
    10381 ccgtgccgtg gggtaatacg tctgtaaaat catcgctatt actgattaat accggcgtcg
    10441 tcagatcaaa tccggcctcg cgaatagcag ggatatcaaa agaaatcagc cgatcgcctg
    10501 tattgacctt gtcacccacg ttgacgtgag cggaaaagaa tttgccgtcc agttttacgg
    10561 tgtcgatacc gacatgaatc aggatctcca caccatcatc tgactcaatg ccaatggcgt
    10621 gtaatgtggc gaacaacgaa gcaattcgac ccgcaaccgg agaacgcact tcaccaaccg
    10681 agggcagaat ggcgatacct ttacccagca ggccactggc aaacgtggta tcagcgacgt
    10741 gaatgagcgg cacaatctct cccgtcatcg gtgaacagat accgccctgc tcaggtggtg
    10801 taataacctc tggtgttttc tcttgcgggg caccctgcgc tggctgacgt ttactggtga
    10861 tgaaatgaag catcaacgta ccaacaaatg cgcaacctat ggcaatgaca ccgccaataa
    10921 cgctggccca gacggtgaaa tcaatccccg ttgacgggat ggtctgcatg aaggtgaaaa
    10981 tacttggcaa accaaaggag taaactttcg tttgcgcgta gccaatgatg gtggccccca
    11041 atgctccact gatacaggcg ataacaaagg ggtacttacg cggcaggttg acgccatata
    11101 ccgctggttc ggtgatacca aacagacccg tcaacgccgc tgatcccgcc accacttttt
    11161 tctgcgcatc gcgttcgcag aggaagacgc cgagcgccgc cccgacctgc gccataatgg
    11221 cgggcattaa cagcgggatc atggtgtcgt agcccagcac ggtgaagtta ttgatacaca
    11281 gcggcaccag gccccagtgc agtccgaaca tgacgaagat ttgccagaag ccgcccatta
    11341 ccgcgcccgc aaatgcagga accgcctgat aaagccagag ataaccggcg gcaatcattt
    11401 cgcttatcca ggttgatagc ggccccacca gcagaaaggt gacgggtgtg ataaccatca
    11461 gacatagcaa tggtgtgaag aaatttttga ttgccgacgg taaccacgca ttaagtcggc
    11521 gttccagaat gctgcacaac caggcagaaa aaataatggg aataaccgat gacgagtaat
    11581 tcaacaatgt gaccggaata cccaggaaat ccagccccgg cgcatccgct ttttgcccgt
    11641 tctcgaaggc agtcagaatt aatggatgca ctaacgctcc accaatcacc atggcagtaa
    11701 atggattacc gccgaagcgt ttgcccgcgg tgtatcccag gattatcggg aagaacccaa
    11761 acaaggcatc actggcgctg aataaaatta aataagtacc actttgttcg gtcgtccact
    11821 gaaaagtgag cgccagagcc agcatacctt tcaggatccc ggttgccgcc atcagaccga
    11881 tcagaggcgt aaaaatacct gaaataacat aaacaaagcg gtttagcaga ttacctttct
    11941 catcattttc cggtgcctgt tgcgcttttt cgccaaggcc tgccacgctg ttaaccgcca
    12001 ggaagacatc ggccacatgg ttacctatga ccacctgaaa ctgaccaccg ctttccacca
    12061 ccataataat accgggggtc tttttcagta cctcagcttg cgctttgctt tcatccttta
    12121 acttaaaacg taatcgcgtt gcacaatgca tcagactcac aatgttatct gcgcccccga
    12181 ctcctgcgac tatttttctg gctaactccg tcataacttg ccctctaccg ctttgcggca
    12241 aaactccaaa aaaaaacctg aaaaaaacgg cctgacgtga atcaagcaat ttttttcagg
    12301 ttttgcccgc ttagtgcggt aacaatcctt tactcagtaa taatatttca gtgttctttg
    12361 cgcacgcgct ctatatttat ggctaaaaac ataatctctg cgggtgaaat tttacgttga
    12421 tactgcaaac caataaaaat ggcgatccgt tccgcacatt gccatgcttg cgggtaattt
    12481 tgttttactg cttgttgtaa tgattcatca ctatcgttaa ttgaagcatg ttcaagaata
    12541 cgccaggata aaaacttcag atgtgtaacc agtcgctgat aactcaagct ttcttcctgg
    12601 taattaaggc tgaactgaaa ttttattaat tgcagcattt cgcgcattaa ctgcgtgaca
    12661 cctgcaacat cctccatatt tccgctcatt tgggcactga ccagatgcat ggcaataaag
    12721 cccacttcat ctttcggtaa ctgcacgccc aaccgtttat caataatggt taacgcttct
    12781 tcccctagct ggaactcttt cgggtaaagc cgctggatat cccacagcaa cgggttgggc
    12841 agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
    12901 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag
    12961 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
    13021 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
    13081 ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc
    13141 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
    13201 ataaacaaaa aaacccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa
    13261 tgcagtaaca atccagtcat ttattaatgg tttttataac gaacatccag gttcggaaat
    13321 taatttaatt gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat gaccatgctc
    13381 gcagttatta actttgtgta attttaggaa tttataaagt tatatataac aaat
//
LOCUS       DAJTYF010000108        13054 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTYF010000108 DAJTYF010000000
VERSION     DAJTYF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.108"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..1136)
                     /locus_tag="OO590_003202"
     CDS             complement(join(<1..887,887..1136))
                     /locus_tag="OO590_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6638473.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSGQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYW"
     gene            complement(1212..3461)
                     /locus_tag="OO590_003203"
                     /pseudo
     CDS             complement(1212..3461)
                     /locus_tag="OO590_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(3485..4108)
                     /locus_tag="OO590_003204"
     CDS             complement(3485..4108)
                     /locus_tag="OO590_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR6638474.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVINH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(4105..5793)
                     /locus_tag="OO590_003205"
     CDS             complement(4105..5793)
                     /locus_tag="OO590_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCR6638475.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAVGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(5942..8569)
                     /gene="gyrA"
                     /locus_tag="OO590_003206"
     CDS             complement(5942..8569)
                     /gene="gyrA"
                     /locus_tag="OO590_003206"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6638476.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            8716..9438
                     /locus_tag="OO590_003207"
     CDS             8716..9438
                     /locus_tag="OO590_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6638477.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(9566..13054)
                     /gene="yfaL"
                     /locus_tag="OO590_003208"
                     /pseudo
     CDS             complement(9566..13054)
                     /gene="yfaL"
                     /locus_tag="OO590_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2983 a         3620 c         3219 g         3232 t
ORIGIN      
        1 ccagtatcga ttttccgatt cgtttggggg taacccaccg ttatattcgt gcggtcttag
       61 tgcgctgtaa tatccaacga tatagtccgt tatggcgtga gctgcctcgc tgaagcttac
      121 gtaacccacc accggcatcc attcgttctt cagactcctg aagaagcgtt ccattgggct
      181 gttatcccag cagtttccgc gccggctcat actctgtctg atctggtatc gccacaataa
      241 ctgccggaac tgcctgctcg tataatgact gccctgatcg ctgtggaaca tcaccccgcc
      301 gggcttacca cgggtttccc atgccatttc cagcgctttc atggtgagcc tgctgtccgg
      361 cgagaacgac atggcccagc ccactggttt tcttgcgaac aggtcgagaa caacggcgag
      421 gtacgcccag cgcttacccg tccagatata ggtcacatca ccgcaccaca cctgatttgg
      481 ctcggtcacg gcgaactgcc tttcaaggta gttagggata gcaacatgtt catgaccacc
      541 acgtttatac cggtgagtcg gctgctgacc gctgaccagc cccagctctt tcatgagcct
      601 gccagcaagc cagcgtccca tctggtagcc tctccgggtt gccattgtgg cgatgcttct
      661 tgctccggcc gaaccgtggc tgatgccatg tagctcaagt acctgactgc gtaatacagc
      721 ccgtctgccg tctggttttt caggacggtt tttccagtat ctgtagctgc tgcgatgaac
      781 cccgaacaca tggcagagtg tgaccacagg ataatgcgct ctgagtttcc cgattatcga
      841 gaactgttca gggagtctga catcaagagc gcggtagcct tttttaatat ttcattctcc
      901 atttcaatgc gttgtagctt tttcctgagc tcacggattt caatttgttc cggggtaatg
      961 ggggaggctt ttggtgtttt gccctgacgc tcatcacgca gttgtttgac ccatcttgtc
     1021 attgtggaaa ggccaacatc catagctttg gcggcatctg ccaccgtgta tttctggtca
     1081 acaaccagtt gagcggattc gcgtttaaac tctgcgctaa aatttctttt tttcattgga
     1141 gcacctgtgt tgttctgagg tgagcatatc acctctgttc aggtggccaa attcagtgtg
     1201 ccacttcatc attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc
     1261 acccggatat caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga
     1321 ccattacggg tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactgga
     1381 acccgggctt catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc
     1441 gaaagcagcg actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt
     1501 ggctccggga aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc
     1561 gccacgatat ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt
     1621 aacccagcga gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta
     1681 gcgaaattga cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg
     1741 tcttcgagac gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca
     1801 taacggaaca caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa
     1861 tgcgccaggc cgcgttcaat gaggagctct ttggtggtgg tgacgcgata cgccgcgccg
     1921 tcactggcgg agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac
     1981 gccacataac cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt
     2041 ccggcataac gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc
     2101 acgcgggcat tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc
     2161 gcttcgccag ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg
     2221 atgtagtttg ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac
     2281 tcataacctc cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc
     2341 gcatccagcg tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc
     2401 ttcgccgggg cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc
     2461 actttaacat cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag
     2521 cgggtgttgt agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca
     2581 gcatccttac ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg
     2641 ctgtcatcgg tcaccccgcg ggtcatcacg ccaagaccgt cagtccacaa gatctctgag
     2701 ccgggcttcg cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac
     2761 actttgctaa gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct
     2821 accatcgcct cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta
     2881 cccggctgcg gcgagatgaa attgctggat gcgccttcca gttttatccc ttgctgcggc
     2941 tcgaccggtt tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc
     3001 agcggggaaa actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga
     3061 tgtaattcgg gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc
     3121 atcacgcggc gagatttgcc gtaccagtta tcccacagct aggtcagcgt attgttcagc
     3181 ccgtcgccca gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa
     3241 aatgccatcg ggtcaggaat acgatacagg cgaacgtcca cgccgccgta ctcttccatc
     3301 tgataacgcc gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg
     3361 ctaaaactgc tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg
     3421 gaaggaagcg aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac
     3481 gctgctatcg cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt
     3541 tgggtatcca tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc
     3601 cgttgtcagt tttcgtggcg cttccggtgt ggttgatgac gtaacgcccc atccagacca
     3661 ttaagtgctg ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta
     3721 tgtcttggcc aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc
     3781 cgtttttccc gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg
     3841 tcatctctgg cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt
     3901 gaactttcag cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc
     3961 agcgtggact tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa
     4021 acagcctgga ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc
     4081 aaatcagcgc cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca
     4141 ggcgacaccg ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc
     4201 gtccagcttc ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt
     4261 cggcagactg gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata
     4321 gagcggaacg atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag
     4381 tgtttgcagg gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg
     4441 catcgccagc gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg
     4501 cgctttcgga tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc
     4561 ctgctgagtc tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat
     4621 attttgcgta aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc
     4681 gacaaacagc ggggtttgca attttgagtc ttcataccag cacagcccca cggcaccgtc
     4741 taacgcccca ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat
     4801 accgtgtgaa tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac
     4861 cggcgtaaaa tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact
     4921 gtgccagccg tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg
     4981 gtagccaaaa cccagcaacc tggcgctggc cacgatgcgc aggcgtactg gcgttttttc
     5041 ggctgtacgc tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc
     5101 acttgcgatc gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact
     5161 ggaaaacacc agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata
     5221 gcgcaactga taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt
     5281 gcttaactgg ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc
     5341 actgcgctgg atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc
     5401 cgcctgcgcg ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg
     5461 ttcatagaca atgcggcgaa tgctgccttc aatgcccaga cgatctgcat gattctgata
     5521 atagaaaacg aaatcctcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt
     5581 gggaagctgg ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg
     5641 cagattgttg tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg
     5701 caacgcccag acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa
     5761 ccgccagcct ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc
     5821 attttttacg ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg
     5881 cagcctggac ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa
     5941 attattcttc ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt
     6001 ccgcggcact gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct
     6061 gcagacccac tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac
     6121 ggcccacgat gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca
     6181 tcatgatctg gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg
     6241 taaccttgat ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg
     6301 cagtacgttt accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca
     6361 cgatcagaga gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat
     6421 tgcagcccat cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga
     6481 acagcattac ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa
     6541 ccagtttgat cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt
     6601 tcttcacagt accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca
     6661 ccggcaggat cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac
     6721 gaccgcgcgc gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac
     6781 cacggctgga gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa
     6841 agtcttcttc tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt
     6901 attcagaaag cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt
     6961 cctgggtgat cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac
     7021 gtttgtcacc gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac
     7081 gatcggcgct accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt
     7141 cgtcgagcag tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg
     7201 cctgagcttg ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca
     7261 gccattccgg acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca
     7321 gctgccacgg attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga
     7381 tcagttcgat gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag
     7441 cacgatcgcg agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt
     7501 gacgaacaaa cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca
     7561 atgccaccat gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga
     7621 gcacaacttc accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt
     7681 cagactcgtc acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa
     7741 tcttctcgat caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt
     7801 cacgtccggt tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc
     7861 gaccggtacg gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga
     7921 agtccggccc cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat
     7981 acgccagaca accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac
     8041 ctacggcgat accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt
     8101 cgggaatttt ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat
     8161 cggccatcag ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg
     8221 cagagtcgcc gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg
     8281 agaatggctg cgccatacgg acgatcgtgc cataaaccgc caagtcacca tggggatggt
     8341 atttaccgat tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat
     8401 tgcctagtac gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga
     8461 catctggcag cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta
     8521 gctcttcctc aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat
     8581 ccctctactg tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg
     8641 taaacctata cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac
     8701 ggagtagaag tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccatgaa
     8761 gagatcgcta aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa
     8821 ccgttgcacc gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta
     8881 tttggcaaaa aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg
     8941 cgcgaaggcg cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag
     9001 ctgcacgcgc tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac
     9061 gcggcaaaac atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta
     9121 cccgatccgc agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc
     9181 tttttctcga cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa
     9241 tatattttgc gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca
     9301 gagctgcttg gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat
     9361 tacaacccga tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg
     9421 cacacgcaga ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca
     9481 gcgtcttatc aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc
     9541 ggcaattgtg atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg
     9601 tctgggcaaa atcatcactc cctttctgcc acgccacgct gccgcgcaga gacactcgct
     9661 gactgatatt gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac
     9721 tgatagtgct gccatcttct tcaatttccg tcgcatgggg atcgtgataa taattcagat
     9781 ctaatgttgg tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct
     9841 gaatatcatc cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct
     9901 gctgcacgcc ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta
     9961 accactgata cccggcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc
    10021 catcttcatg ttcggaaaca tcattgttaa accacgcgta ttgcagccag ctatccagcc
    10081 aggccccctg cttctgatta ccgtgctgat accagcttga ggtcagccca acggcataac
    10141 cgtggttctg gttatcggcg cgagttccgg tcatattcga gcggctgtcg ccctgattat
    10201 cactgtagcc accaaccgcc ccaagcatcc actcgccatc cgtgccccag cacccgctaa
    10261 acaagccgcc gctaagcagc accgtagaag tgtcttcctg ctgagccagt tgccctgccg
    10321 ccgtgtaatg ataacgtccg ccgataacac gtaaattcag cgtctgacca tcgccaccag
    10381 cgtgatcgcg tcgctccatc ataaacgcct gatttgccgc ccgcaggtta ttgagataac
    10441 cgccaacttt ggcattcagc accggctggt aagcaggcgt aggggccggg tcgggggttg
    10501 ggtcaggatc cggcgtggga tcaggatcgg gagtcgggtc tggctcaggt ggcgatggcg
    10561 gcgttacttc ttgcgatcgc agataccagt cgttgttatc ttccactagc gtgtagtcat
    10621 acgctcccat gttgacgtag ccactgccag ccagactgaa ctgtgcattg ttttgaaact
    10681 gcgtgggatc agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc tcaccaatcc
    10741 ctgtaatgga gttaaccacc acagttgtgt tgccagcagt attaccgttc atcaccaatt
    10801 gatcgcttac cgagtcatcg ccgtttaatt cgctgtcgaa tagtagcgta ccgccgccgg
    10861 tataatcacc gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg ctaaccgcac
    10921 cggacgtatt ggtcagatca ccattaaccg tcacgtcagc aagataggtc aggctgtcag
    10981 tttcaaggtt ggtattaagc tcgcctgtca acgtcacgcc atcggtagca tttaccagcg
    11041 tcccatcgcc gttaaatagt gaggcattaa gggcgacgga agtatcctgc ccggtcagac
    11101 gcaaaaccgt accatcgctg atgtcgatag tgccgctgga agtagcatca attgactgaa
    11161 tatcctgatc cgcgccagta acgaacgttg ccccatcacc aacccacagt gacgaagcat
    11221 aaggaaagat atccgcaaca tcgcctttca gggtgccttc ttcgacacgt accgccgact
    11281 gcgttgtacc gctggcggtc agctccagcg tacccgcccc cgttttagta aatgtgctac
    11341 cctcatcctg atgctgcccg ctgctgtcag ccatcagtgc gccccactgc gtgtctaccc
    11401 ccgcatcaac tgccacttca ccgtcggcgc gcatttcaat atcacgccca tgctcagtcg
    11461 tcgtagagcg agtatcgaca ttggcagtaa tctccatcac gctttcttgt tacttatcgg
    11521 taaagataac ctggcggtta tagtgggtat ctccaagctg cgagttgtcg ctcaccatca
    11581 gcgtaccgga ggcgatttgt gttgtgccca aataactatt attgtttgcc agacttacct
    11641 gcccgtcgct cgcatcgacc accacgtaga cgccatcttt cccgtcaccg ccgatatgca
    11701 aattgtcgcc acctgcaaga tctaccacat cctgactgcc gataaccgtt ccgctgctgc
    11761 cactgacttc aaggccatca ttgtatgatg tgccgctctg ccaagtggag aaatcagaga
    11821 gatcgagcac accgccgttt aacacgatcg actgcggatc gtcctggaga gcggtaagat
    11881 ctgccgcgtc gccttccagc gaaagcaccg caccatcatc aacgactata tcgccggtta
    11941 gcgccatcga ctgcgcccct gccagcacgt agctgccgtt ttgcgcaatg gtgagctgac
    12001 cagcaccttc gatgatgcca gcaaaactgc cctgattaac agtaacgttg ccaccagcat
    12061 cgatatttaa agtgccattc tgaaagcccg tcaatgcgtg cacaaaagtt tgttgggtcg
    12121 agccaacgtt tagctcagcc tgattctgat actgatcaat actccctatc gtcagaccgt
    12181 agcagtcttg cggatcgtcc tggcaatgcg tatcgccgac attcatcagg gagttgctgc
    12241 gacccagggt aacttcaccg ttttcaatct gcatctcacc agtaaagtca ttgttatctg
    12301 cattaagtac cagatcgccg gaacctgttt tggtgattaa cccggtacca gcaatagagt
    12361 caacagctcc gtcattctct gtattgccga taaccagcgt ttttccgtcg gcaatatcaa
    12421 aggtaacttc acttaagccg agatacataa agccacccgc cgcagtggaa ggaccatctc
    12481 catagcctgc tgcgctattg ttctcatcga ctaacacgcc gccgttctgg ctgtagctgt
    12541 catcaacaga aatatcaata agatagggag ccctcacgct attggtatat atcgccccgc
    12601 cataaccttc ggctgtgtta tttgtaaagg cggtgttatt tattatcgta taacctgaag
    12661 gatgcttgct gtcgctatta ttatcggtaa catcgattgc cccgccatcg ccatcactgt
    12721 aacttgttga tgtatatgcc tggttattgt taaaaacatc atcacttaaa tagacgtcat
    12781 tattaatggt ataaattgcg ccacctttgc catcattagc gatattattg cgaaacatgg
    12841 cgttagtgac acgtaaatcg acggcaccag tatcattagt accagaagaa tagattgcgc
    12901 cactatagcc gcctgcgacg ttaccggaaa aaataacatc agtaagattc agcgttgagt
    12961 tttctttagc aaatatggcc ccgccgttat tatattctcc tgtaacggtg ttattggcaa
    13021 acagagtcat tccagtttca ttttctggta atag
//
LOCUS       DAJTYF010000109        12871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTYF010000109 DAJTYF010000000
VERSION     DAJTYF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.109"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..394)
                     /gene="yafO"
                     /locus_tag="OO590_003209"
     CDS             complement(<1..394)
                     /gene="yafO"
                     /locus_tag="OO590_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR6638478.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMR"
     gene            complement(397..690)
                     /gene="yafN"
                     /locus_tag="OO590_003210"
     CDS             complement(397..690)
                     /gene="yafN"
                     /locus_tag="OO590_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR6638479.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(742..1797)
                     /gene="dinB"
                     /locus_tag="OO590_003211"
     CDS             complement(742..1797)
                     /gene="dinB"
                     /locus_tag="OO590_003211"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6638480.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1868..2653)
                     /gene="lafU"
                     /locus_tag="OO590_003212"
     CDS             complement(1868..2653)
                     /gene="lafU"
                     /locus_tag="OO590_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6638481.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            2625..4337
                     /locus_tag="OO590_003213"
                     /pseudo
     CDS             2625..4337
                     /locus_tag="OO590_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4554..5051)
                     /locus_tag="OO590_003214"
     CDS             complement(4554..5051)
                     /locus_tag="OO590_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6638482.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTIQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(5227..5748)
                     /locus_tag="OO590_003215"
                     /pseudo
     CDS             complement(5227..5748)
                     /locus_tag="OO590_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            6132..6872
                     /locus_tag="OO590_003216"
     CDS             6132..6872
                     /locus_tag="OO590_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6638483.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6843..7610)
                     /locus_tag="OO590_003217"
     CDS             complement(6843..7610)
                     /locus_tag="OO590_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6638484.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(7816..8394)
                     /gene="lpcA"
                     /locus_tag="OO590_003218"
     CDS             complement(7816..8394)
                     /gene="lpcA"
                     /locus_tag="OO590_003218"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6638485.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            8634..11078
                     /gene="fadE"
                     /locus_tag="OO590_003219"
     CDS             8634..11078
                     /gene="fadE"
                     /locus_tag="OO590_003219"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6638486.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDTMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(11121..11567)
                     /gene="ivy"
                     /locus_tag="OO590_003220"
     CDS             complement(11121..11567)
                     /gene="ivy"
                     /locus_tag="OO590_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6638487.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            11748..12518
                     /gene="yafV"
                     /locus_tag="OO590_003221"
     CDS             11748..12518
                     /gene="yafV"
                     /locus_tag="OO590_003221"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6638488.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            12627..12859
                     /locus_tag="OO590_003222"
                     /pseudo
     CDS             12627..12859
                     /locus_tag="OO590_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         3052 a         3331 c         3412 g         3076 t
ORIGIN      
        1 aacgcatgcg aaacgcttct gccatttttc caattttatg catttggttg ttatctcgag
       61 ccagtttatg aggttcaggc ttcagaatgg caatgagcaa ccatgcttgc tcatcaaacg
      121 caccctgaca atataccaaa tgcgcttcgt cattcgttct gctgaattgg cgcaactgtg
      181 gcggaaatgg attattcaca tttgccagat gaatatgagc aactcgctca aatttgatta
      241 atggccaggt aaacgagtcg tcgtagagtg catcgcgacc aaatatatct ggcaaaacac
      301 cgtcacgctt ataggaaata aaatccgccg ttaacgcatc aagttcctct gctgtaagtt
      361 gcaggcgaat aagttttgtt ttgaataccc gcatccttat tccttaaagt cattgaaatc
      421 atcatccgtc atatcattaa gatattgttt agcgcggcgt gtaatttcct ctaatcttgc
      481 agcacttgga cgaaagcgcg cctttatggg ctttttctcc tcttgttcag caagcatgtc
      541 cattaacgct tcgaatgcgc tggcacttaa gagatatcct gcggggcgat tattagaaag
      601 aaccgcaact ggttgatcaa taaagtattt agctgggttt ttacgtaact cagtgatatt
      661 gaccgatttt tcagcgagaa tccgatgcat acagtgatac cctcataata atgcacacca
      721 gaatatacat aatagtatac atcataatcc cagcaccagt tgtctttcca tttgcgggtc
      781 aagcaacgtc acatgcagcc ccaccagacg cacaccgcgc ccgccgcggc gttcatccca
      841 ggttttacgc gcggtggcga ttaagtcagc tttattcagc cgcggccaga cgtgctcctg
      901 ggtggtctgc tgaaaatcgt cgaactttaa tttcaccccc tggcgagcaa tcagtaaatc
      961 aggttttacc tttgccagac ggcgttcaag ttccggatac agccgctcga taatcgcttc
     1021 acattcagac cagtgatgaa tatcttccgc catcgtgcgt tccacgccga cggattttcg
     1081 taaccgttcg ctgttaacgt cgcgctcgtc aatcccctga ctacgctccc acaaaatgcg
     1141 gccaaatttg ccaaagcgtt taagcagaat caccagatca cacttttgta catcaccgca
     1201 ggtccgcagc cccatcgctt ccagttttgc cgccgagact ttgccgacgc cggggatttt
     1261 ttccagtggt aaggtttgta aaaatgccgg aacttctgcc ggcgtaatca caaactgacc
     1321 gttgggttta ttcatgtcgg aggcgatttt ggcgagaaac tttactggtg ccacgcccgc
     1381 agatgccgtc agttgcagct cgttgaagat cgtctggcgg atttcctggg cgatgagggt
     1441 cgcagaaccg tggcaatgga cgctatcggt aacatcgaga taagcctcat ccagtgacaa
     1501 cggttcaatg cgcgaggtgt agcgcgagaa gatttcacgg atatgatttg aggcttcttt
     1561 gtaggcgtca aagcgccccg gaagcaaggt gagatgtggg cataatttga gcgccatccc
     1621 agtaggcata gcgctacgta cgccaaattt acgcgcggga taattggcgg tgctgatcac
     1681 cccccgacgt tcgcggctgc cgccaatagc aatagggata tcgcgcagag cgggattgtc
     1741 gcgcatctcc accgctgcga aaaagcagtc catatccaca tgaatgattt tacgcattgc
     1801 tcacctctca acactggtaa agtatacagt gatttcaggt tttgagaaat gcgtaaagat
     1861 tcagcattta gcgcattcgc tgcgaaagca cctgctgctt atccagcttt tgcaccagcg
     1921 gctgcaccac tttatcccca tgctgaccaa agtattgata cagcgtatcg gacgcacttt
     1981 tggtcagtac cataatctca atacgccgat tgcccgcgct ttgcggattc ttcgcgtcca
     2041 gcagcatctg gtccgccatt gcgcttacct gcatcacttt atcttccggc attccggcct
     2101 cttccagcac ccgacgagct gaaagcgcgc gatcgcccga aaggttccag ttgttgtaga
     2161 tattgttttt gtaggccatc gcatcggtat gcccggtaat aataattttg ttatagagcg
     2221 agtcgaacac tggcgcgagc tccaccagca aagttttaaa gaacggcata atctgcgcgc
     2281 tgccgcgttc gaacatattg cgattctggt cgtctttaat cagcacgcgt aatccctggg
     2341 gaacaatctc catctccaga ttcgcttcca tatgcgcatc acgggcaatg gtgttgatac
     2401 tggttgccaa ctcgccaagc tcggtggcgg atttcttctt cagcagcgcc gttttctcat
     2461 tcacatcacg cgcttttttc tccgtctctt ccggcaccgc cacggtggca ggttttggcg
     2521 gatgagacgg gctgatttta tttaacggcg acagcccacc accgttaaaa atcgactgcc
     2581 cgtgcagcgc ggcgataatg ctttcacgct cggatttact gacgctgttg acaatcaact
     2641 ttatcgtcgt caccaaagag gccgagcgta tttccgaggt ttctgcccgc tttaccctgg
     2701 acgcgatgcc cggcaaacag atggcgattg acgccgatct taacgccgga ttgatcaacc
     2761 aggcgcaggc gcaaacccgg cgtaaagatg ttgccagcga ggccgatttc tacggcgcga
     2821 tggacggggc atcgaagttt gtacgcggag acgccatcgc cgggatgatg attctggcga
     2881 tcaacctgag cggcggcgtc tgtatcggca tcttcaaata caacctgagc gccgacgccg
     2941 ccttccagca atacgtgctg atgaccatcg gcgacggcct ggtggcgcag atcccttccc
     3001 tgctgctctc caccgcggcg gcgattatcg tcacccgcgt cagcgacaac ggcgatatcg
     3061 cccatgacgt gcgccaccag ttgctggcaa gcccgtcggt gctctacacc gccaccggga
     3121 tcatgttcgt gctggcggtg gtgccgggga tgccgcatct gccgtttttg ctgttcagcg
     3181 ccctgcttgg ctttaccggc tggcggatga gcaaacgccc gcaggcggcg gaggcggaag
     3241 agaaaagcct cgaaacgctg acccgcacca tcactgaaac cagcgaacaa caggtcagtt
     3301 gggaaactat tccgctgatc gagcccatca gcttaagcct cggctacaag ctggtggcgc
     3361 tggtggacaa agcgcagggc aacccgctca cccagcggat tcgcggcgtt cggcaggtga
     3421 tttccgacgg taacggcgtg ctgctgccgg agatccgcat tcgggaaaac ttccgcctca
     3481 agcccagcca gtacgctatt ttcatcaacg gcattaaggc tgatgaagcg gatattccgg
     3541 cggataaact gatggcgctg ccctccagcg aaacctacgg cgagattgac ggcgtgctgg
     3601 ggaacgaccc ggcgtacggg ataccggtca cctggatcca gcctgcgcag aaagcgaagg
     3661 ctctgaatat ggggtatcag gtgatcgaca gcgccagtgt gattgccacc catgtgaaca
     3721 agattgtgcg cagctatatt cctgatttgt ttaactatga tgacatcacg cagttgcata
     3781 accgtttgtc gtcgatggca ccgcgcctgg cggaggattt aagcgcggcg ctcaattaca
     3841 gccaattgct gaaagtgtac cgggcgctgc tgaccgaagg cgtttccctg cgcgatatcg
     3901 tcaccatcgc caccgtgctg gtcgccagta gcacggtgac taaagatcat attctgctgg
     3961 cggccgatgt gcgcctggcg ctgcggcgca gcattactca tccgttcgtt cgcaagcagg
     4021 agctgacggt gtatacgctg aataatgagc tggaaaatct gctgaccaac gtagtgaatc
     4081 aggcgcaaca ggccggcaaa gtgatgctcg acagcgtgcc ggttgacccg aatatgctca
     4141 accagttcca gagcacgatg ccacaggtga aagagcagat gaaagcggcg gggaaagatc
     4201 cggtgctgct ggtgccgccg cagctacgcc ctttgctggc gcgttatgca aggttgtttg
     4261 cgccggggct gcatgtgctg tcgtataacg aagtgccgga tgagctggag ttgaagatta
     4321 tgggggcgtt gatgtaagaa tccggcatcg gtgcacaatt gccggatgcg gcgtaaacgc
     4381 cttatccggc ctaccgttca ggcacaagtt tttaggcatg ataagacgcg ccagcgtcgc
     4441 atcaggcatc tgcgcataac tgccggatgc ggcgtgaacg ccttatccgg cctaccgtta
     4501 aagcacgatt tgtaggccgg ataagcgtaa gcgcatccgg caccagcgca cagttataaa
     4561 atgatacgct ccccggcact taaatccgtt atgtctcccg cccaatcgat gggatataac
     4621 cctcgcgcga catcgcgatg gaacgttgag aatggccaat cactcacctg ctttacccag
     4681 ccatgtttct ctggatttat ataaatataa tcaacatgat ggcgataatc ttttgtattg
     4741 cgaatggcat gctcccaaaa acgcggttgc cagatatttt tcaatccgca agcatgggtg
     4801 aactgctttt taatctcccg ccagcgagag gaaaaatcat catcgctttc gggtaatgtc
     4861 catatgcaat gcatatgctc cggcaaaacg acccaggcgt taatttcaaa aggtctgtct
     4921 cgcttaacgt taataatggc gttacggagc gtctggaact ggatggtcag aagatggctt
     4981 cgacgattgc gtaaatttac cgtgaaaaac catgttcccc ccttgatgta ataacgacga
     5041 tattccgaca tagcgactcc tttttctcaa cggtaagagg aaaaggaaag gccgacaatg
     5101 acgctgtgtt atatcgggaa agcggctcgc aaaaacgcac agatttgtag gcatgataag
     5161 acgcgtcagc gtcgcatcag gcatccgtgc acaaccgccg gatgcggcgt aaacgcctta
     5221 tccggcctac aaaatcatct cttccgtcaa aatcctgcgt gcgcctaaaa aatggtcctg
     5281 ccagaaaggt tcggctaacc ggcttacccg aatggtttcc ccggtgcgca gtgactcaat
     5341 aaactgaccg tcgcccagat acacccccat atggtcggca atctcgcggc tgtggatatg
     5401 gaaaaatagc aaatctcccc ggcgcaggtc gttattcgcc acaatcgttg cccgacggta
     5461 gtggtacatc tcattagccg tgcgcgggag tttcgcctca aggatcttgt tgtaggcata
     5521 aaaaactaac ccactacaat cgaagccttg atcgggccgc gtgccgcccc agacgtaagg
     5581 cttgccaagc tgctgctcca ggcggtgaat cgccacttcg gtgatgttat gcaggtgatc
     5641 gctgcttaaa aagtggttgt tttccgccag ctcctgccag cgtctgtcac tcgccttgag
     5701 cggcgcagga aaccattctg gatgctgttt tatctgctcc cggttgtggt ggcgctggcg
     5761 agtaaaggga gcaaaacgca taacgaaaac acgcctggat gcaaaaatct gccgagcaag
     5821 aacgaagaca tgaaagacat aaaccggctg aatgaaggtg agaattttaa aagaccggag
     5881 gagtaaacgg gattattaat tgcttatttg catattacta ttgagatttc ataatattag
     5941 gaaataaatg atacatgcgc ttttcggact attgaaaact gcccctgtgc taaatttgta
     6001 aacccagcgc cgacaggctt gcgtaaaact cttttggtga taatgtgagc cttccaaatc
     6061 cagactattc cgatttggga gccattttgc cgtcctggcg tgtgtaaccg ttttatcaag
     6121 gaataagcag tatgcgtaaa atcgcattaa ttcttgcgat gttgttgatc ccgtgcgttt
     6181 cgtttgccgg tttgctgggc agcagtagct cgaccacgcc agtgagcaaa gagtataagc
     6241 agcagttgat gggatcccct gtctacatcc agatcttcaa ggaagaacgt acgctcgatc
     6301 tttacgtcaa aatgggcgag caatatcaac tgctcgacag ttataaaatc tgtaaatatt
     6361 ccggcggctt agggccaaaa cagcgtcagg gcgatttcaa aagcccggaa gggttttata
     6421 gcgtccagcg taatcagtta aaaccagaca gccgttacta caaagcgatt aatattggat
     6481 tccccaatgc ttatgaccgt gcgcatggtt atgaagggaa atacctaatg atccacggcg
     6541 attgtgtatc catcggctgc tacgcaatga ccaatcaggg tattgatgag atcttccagt
     6601 tcgttactgg tgcgctggtg tttggtcagc cgagcgtgca ggtgagtatt tacccattcc
     6661 gcatgaccga cgccaatatg aagcgccata aatattccaa ctttaaggac ttctgggagc
     6721 agctgaagcc gggctacgac tactttgagc aaacccgtaa gccaccgacc gtttctgtgg
     6781 ccaatggccg ttacgtggtc agcaagccgt taagccacga agtggtgcag ccacaactgg
     6841 catcaaacta cacgctcccc gaggcaaaat aagcgccatt cgcctggcat aattttttgc
     6901 caggtttcat tgcccgtcag cggctgtgtc gcaatcaccg tgaccacatc attcggtgtg
     6961 gtctgcgagc tgaagtcgat ttccacatcc tgatccagca acgttgccac gccaaacggc
     7021 gcgcggcggg tgatccagtg taaattagtc gagcaatacg ccattacata gcgcccgtcc
     7081 gaaagcagca tgttgaaaac gcccttcgcc cgcagttcat ccgccagtga ggcgatatat
     7141 ttaaatactg ccgccatgtt gcccggcgtg cgcgggtaac gctgcgttaa tttatgcagc
     7201 agccagcaaa aggctttttc gctgtcggtt tcaccgaccg ggcggaagtt gccggtttcc
     7261 agtgatttgt agcccgtcag ttgtccgtta tgggcataag tccagttacg cccccataac
     7321 tcgcgggtaa atgggtgagt attttccagc gccacctcgc cccgattagc ctggcgaata
     7381 tgagccacca ccgaacagga ttttatcggg tagtcctgga caagtttggc gatgggggaa
     7441 ttaaagctgg gttgtggatc tttaaatgtg cgacagcctt taccttcgta aaaggtaatg
     7501 ccccagccat ctttatgtgg cccggttcca ccaccacgct gtacaagtcc ggtgaaacta
     7561 aagcagatat cggtagggac gttggcgctc atcccgagca attcgcacat gggtaacctc
     7621 cacaacagcg ggagcgcatg gaccccggcg aagattgatt taagaatttt caggtcggat
     7681 gcggcgtaaa cgaattattc ggcctacggt tcgggtacag atttgtaggc atgataagac
     7741 gcgtcagcat cgcatcaggc atcagcgcac aagtgccgga tgcggcgtaa acgccttatc
     7801 cggcctacgc cagacttact taaccatctc tttttcaatt aactggatca ggatatggat
     7861 cactttaatg tgaatctcct gaatgcggtc ggcataacca aagtgcggaa cgcgaatttc
     7921 gatatccgcc gtgccagcca ttttaccgcc gtctttaccg gtcagggtga tcactttcat
     7981 ccccttctca cgcgccgctg cgatcgcttt gatcacgttt gcagagttac cggaggtgga
     8041 gatccccagc agtacatcgc cttcgcgacc taccgcttca acgtagcggg agaaaatatc
     8101 attgaaaccg aaatcattac cgacgcagga aatatgacta acgtcagaaa tagcaatcgc
     8161 cgggtagccc ggacggtttt cacggtagcg accggtcaac tcttcggcaa agtgcatagc
     8221 gtcgcaatgg gaaccgccgt tgccgcagga aagcactttg ccaccggctt taaagctgtc
     8281 tgctaacagg accgccgcgc gctgaatggc gtgaatattg gcgtcatctt ttaaaaagtt
     8341 agccagcgtt tccgccgctt cgttcagttc gttacgaata agatcctggt acatgaggat
     8401 atccttcagc ataaatgtaa tagacaaaat gcagtgtacc ggataccgcc aaaagcgaga
     8461 agtacgggca ggtgctatga ccaggacttt ttgacctgaa gtgcggataa aaacagcaac
     8521 aatgtgagct ttgttgtaat tatattgtaa acacattgct aaatgttttt acatccacta
     8581 caaccatatc atcacaagtg gtcagacctc ctacaagtaa ggggcttttc gttatgatga
     8641 ttttgagtat tctcgctacg gttgtcctgc tcggcgcgtt gttctatcac cgcgtgagct
     8701 tatttatcag cagtctgatt ttgctcgcct ggacagccgc cctcggcgtt gctggtctgt
     8761 ggtcggcgtg ggtactggtg cctctgacca ttatcctcgt gccatttaac tttgcgccta
     8821 tgcgtaagtc gatgatttcc gcgccggtat ttcgcggttt ccgtaaggtg atgccgccga
     8881 tgtcgcgcac tgagaaagaa gcgattgatg cgggcaccac ctggtgggag ggcgacttgt
     8941 tccagggcaa gccggactgg aaaaagctgc ataactatcc gcagccgcgc ctgaccgctg
     9001 aagagcaagc gtttctcgac ggcccggtag aagaagcctg ccggatggcg aatgatttcc
     9061 agattactca tgagctggcg gatctgccgc cggagttgtg ggcgtacctt aaagagcatc
     9121 gcttcttcgc gatgattatc aaaaaagagt acggcggact ggagttctca gcttatgccc
     9181 agtctcgtgt gctgcaaaaa ctctctggcg tgagcgggat cctggcgatt accgtcggcg
     9241 tgccaaactc attaggcccg ggcgaactgt tgcaacatta cggcactgac gagcagaaag
     9301 atcactatct gccgcgtctg gcgcgtggtc aggagatccc ctgctttgca ctgaccagcc
     9361 cggaagcggg ttccgatgcg ggcgcgattc cggacaccgg gattgtctgc atgggcgaat
     9421 ggcagggcca gcaggtgctg gggatgcgtc tgacctggaa caaacgctac attacgctgg
     9481 caccgattgc gaccgtgctt gggctggcgt ttaaactctc cgacccggaa aaattactcg
     9541 gcggtgcaga agatttaggc attacctgtg cgctgatccc aaccaccacg ccgggcgtgg
     9601 aaattggtcg tcgccacttc ccgctgaacg taccgttcca gaacggaccg acgcgcggta
     9661 aagatgtctt cgtgccgatc gattacataa tcggcgggcc gaaaatggcc gggcaaggct
     9721 ggcggatgct ggtggagtgc ctctcggtag gccgcggcat caccctgcct tccaactcaa
     9781 ccggcggcgt gaaatcggta gcgctggcaa cgggcgcgta ttctcacatt cgccgtcagt
     9841 tcaaaatctc tatcggtaag atggaaggga ttgaagagcc gctggcgcgt attgccggga
     9901 acgcttatgt aatggatgct gcggcatcgc tgattaccta cggcattatg ctcggcgaaa
     9961 aacctgccgt gctgtcggct atcgttaagt atcactgtac ccaccgcggg cagcagtcga
    10021 ttattgatac gatggatatt accggcggta aaggcattat gctcgggcaa agcaacttcc
    10081 tggcgcgtgc ttaccagggc gcaccgattg ccatcaccgt tgaaggggct aacattctga
    10141 cccgcagcat gatgatcttc ggacaaggag cgattcgttg ccatccgtac gtgctggaag
    10201 agatggaagc ggcgaagaac aatgacgtca acgcgttcga taaactgttg ttcaaacata
    10261 tcggtcacgt cggtagcaac aaagttcgca gcttctggct gggcctgacg cgcggtttaa
    10321 ccagcagcac gccaaccggc gatgccacta aacgctacta tcagcacctg aaccgcctga
    10381 gcgccaacct cgccctgctt tctgatgtct cgatggcact gctgggcggc agcctgaaac
    10441 gtcgtgagcg tatctcggca cgtctggggg atattttaag ccagctttac ctcgcctctg
    10501 ccgttctgaa gcgttatgac gacgaaggcc gtaatgaagc cgatctgccg ctggtgcact
    10561 ggggcgtaca ggatgcgctg tatcaggcag aacaggcgat ggacgattta ctgcaaaact
    10621 tcccgaaccg cgtggttgcc gggctgctga atgtggtgat cttcccgacc ggacgtcatt
    10681 atctggcacc gtctgacaag ctggatcata aagtggcgaa gattttacaa gtgccgaacg
    10741 ccactcgttc acgtattggt cgcggtcagt acctgacgcc aagcgagcat aacccggttg
    10801 gtttactgga agaggcgttg gtggatgtga ttgccgccga ctcgattcat cagcggatct
    10861 gtaaagagct gggtaaaaat ctgccattta cccgtctgga tgaactggcg cacaacgcgc
    10921 tgacgaaggg gctgattgat aaagatgaag ccgctattct ggtgaaagcg gaagaaagcc
    10981 gtctgcgcag tattaacgtt gatgactttg atccggaaga gctggcgacg aagccggtaa
    11041 agttgccgga gaaagtgcgg aaagttgaag ccgcgtaatt gaataacgga gccgaaaggc
    11101 tccgtttctt tatccgctaa ttatttaaaa ttaaagccat ccggatggtt ttccaggctg
    11161 ccggtcaacg ccgcgaacag caccgtttta ccatcaatcg aaagcgcatc gttcacattc
    11221 agccaggtga gtttctcttg cgacgttttc tcatcaatag ccgagaacag ccccgtcatc
    11281 tgattagatt tctcggacca catcacagcg atacgttgcg agccacagtc atgcggtttg
    11341 cacgcgctca tcacctgata cgtctcatct cccaacgtta cggtttgtgc gggagtataa
    11401 gtaccgcctt tcatcaccca tgcaggtagc ttatgccctt gcaccatctg attaaacgca
    11461 gctttggtgg tttcgccctt tgcaaggctg ctaatggtta aatcatcctg cgccattgca
    11521 ctggtggcga tgaccagtgc ggcgactgtc gttattgcct taaacatcat tcctcccgag
    11581 cttatcctgc ccatgttatt aacctcctaa agcctggcga taacacgata atttgcaaat
    11641 cagatatttc caatatcagc atttgttatc aatttattct gcgctaaggc aactgcctga
    11701 taaggtgtta aagttataaa acccttcgca taaaggagtt gtacaccgtg cctagtttga
    11761 agattacgct tttgcagcaa ccgttggtgt gggtggatgg tcctgccaac ctgcgccatt
    11821 ttgatcgtca actggaaggt attaccgggc gcgatgtgat cgttctaccg gagatgttta
    11881 ccagcggctt tgccatggaa gcggcagctt cgtcgcttgc acaagatgac gtagtgaact
    11941 ggatgacagc taaggcgcag cagtgcaatg cgctgattgc aggcagtgtt gcattacaaa
    12001 cggagtctgg ttcggttaac cgctttttgc tggttgagcc gggcggcacg gtacattttt
    12061 atgataagcg tcatctgttc cgcatggcag atgagcatct gcattataaa gcgggcaatg
    12121 cgcgagtgat tgtggaatgg cgcggctggc gtattttgcc gctggtgtgc tacgacttac
    12181 gttttccagt gtggtcgcgc aatctcaacg attatgacct cgccctgtac gtcgccaact
    12241 ggccagctcc gcgctctctg cactggcagg cattgctgac ggcccgcgcg attgagaatc
    12301 aggcgtatgt ggcgggatgc aatcgcgtcg gcagcgatgg caacggctgc cattatcgcg
    12361 gtgacagccg ggtgattaat ccgcaaggcg agattatcgc taccgccgac gcgcataagg
    12421 caacgcgtat tgatgcggag ctgtcgatgg tggcattgcg ggaatataga gaaaagtttc
    12481 cggcatggcg ggatgcggat gagtttaggt tgtggtgagt gggggttgtg tttagggccg
    12541 gggctgggct agattacgaa agcccctaac ttaaacacaa tattacgcca gaagtcacca
    12601 tttccaaaac acagcgtcgc tgtaccgtgg gttatctgcc caaccgcagc gacacgccgg
    12661 tgatcaaaat cagcgccaaa tggctgagaa aagcgggatt tgagatcggc acgggcgtta
    12721 ccgtgaagat ctcggaggga tgtttgatcc tgcttgggat aacaacgaag tacaggaact
    12781 gcgcagggaa ctttatcagg ttaaacagtc agttaagggg atgcgagacg ggatgtttag
    12841 tgtgctgaac gaaagttaaa taataaaaag c
//
LOCUS       DAJTYF010000110        12685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTYF010000110 DAJTYF010000000
VERSION     DAJTYF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.110"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO590_003223"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO590_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6638489.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="OO590_003224"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="OO590_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR6638490.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="OO590_003225"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="OO590_003225"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6638491.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGDYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIHHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DTLSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="OO590_003226"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="OO590_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6638492.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGLVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="OO590_003227"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="OO590_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6638493.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAVRAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="OO590_003228"
     CDS             4025..4615
                     /locus_tag="OO590_003228"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6638494.1"
                     /translation="MNEAVSPGVLSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="OO590_003229"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="OO590_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6638495.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="OO590_003230"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="OO590_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6638496.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="OO590_003231"
     CDS             complement(6551..6658)
                     /locus_tag="OO590_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638497.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="OO590_003232"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="OO590_003232"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6638498.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="OO590_003233"
     CDS             7648..7875
                     /locus_tag="OO590_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6638499.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO590_003234"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO590_003234"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6638500.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="OO590_003235"
     CDS             complement(9447..10703)
                     /locus_tag="OO590_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR6638501.1"
                     /translation="MGSNIHGMSCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="OO590_003236"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="OO590_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6638502.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="OO590_003237"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="OO590_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6638503.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12685)
                     /gene="torD"
                     /locus_tag="OO590_003238"
     CDS             complement(12282..>12685)
                     /gene="torD"
                     /locus_tag="OO590_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6638504.1"
                     /translation="RDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVE
                     AGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKILTALRQWLPEF
                     AARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3033 a         3235 c         3193 g         3224 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccggcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgtgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcgacta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcattttg cgcagccgtg gaatacacca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacacgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttaacg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggctt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg tccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgt gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcttg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcctatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacggta atttgattat
     6301 ggtcgcagta accttcgttc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcgggcaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggtgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttattaaa taaccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc tcggcatatt tatctgttct
    10201 ggctggatac tcatattttc atcatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt cgatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaactc
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg aagtggcggt
    11341 attcttcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgta gcgccgtcag tattttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgta
//
LOCUS       DAJTYF010000111        12173 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTYF010000111 DAJTYF010000000
VERSION     DAJTYF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.111"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(126..293)
                     /locus_tag="OO590_003239"
     CDS             complement(126..293)
                     /locus_tag="OO590_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638505.1"
                     /translation="MHFRVTGEWNGEPFDRVIEAEDINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHHAA"
     gene            complement(481..1320)
                     /locus_tag="OO590_003240"
     CDS             complement(481..1320)
                     /locus_tag="OO590_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6638506.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPVAYIFKHPAGKLFWALTDESNKEQADVIPVYAAAPASVVPDNASEPLAYAYKELT
                     PEIMRNHLAVFERYGIAPNDSSTTIQALRIALDGIERSDAMLHGAEPVSQTYKLPPLS
                     SNEVNDAAWKLHNMLTEHGPLNGRQFNNLKGCFYEALKVAMRNYPVTPDGWISCSERM
                     PEDTKMLLAFSQGQIVAAYWNWVMSPIDYKKYRAFTYLSGNILDDVTHWMPLLEPPEQ
                     DGE"
     gene            complement(1322..2008)
                     /locus_tag="OO590_003241"
     CDS             complement(1322..2008)
                     /locus_tag="OO590_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR6638507.1"
                     /translation="MSEINYQALRKAAQLATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVAQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEGKDSKIANLTAERDALREGEMGDARYSNTRAAADIYFQLVEECEIPAGGSLV
                     EYVDDMREKLESAEKRIAELEEREILLPERSSMLHRTDFHEDYQTVMAYKVSEVIDAI
                     RATGIRIKGE"
     gene            complement(1995..2309)
                     /locus_tag="OO590_003242"
     CDS             complement(1995..2309)
                     /locus_tag="OO590_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638508.1"
                     /translation="MTITKQRVEKIIYRHEMGLSSDVTAEEVYDLAVLALNLSNIANL
                     KRYELDMGGCDSCGQDCGADMTEDSDGDYVLFDDVVKLFEFDTTTQKLESPAKEATSE
                     RN"
     gene            complement(2306..2791)
                     /locus_tag="OO590_003243"
     CDS             complement(2306..2791)
                     /locus_tag="OO590_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638509.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVNETNAEYLVRKFAEAEAKCA
                     ALAAENAGLKAALNHEVIPDTAVEAFNETAIIDHDWNDTSEWSWVENDTDVIRAVLEA
                     IKPETPATDAFLAEIERKAIRKFINSIEHILRDKLSPYDTEEMLETMRIFLEEQGGEQ
                     K"
     gene            complement(2788..2952)
                     /locus_tag="OO590_003244"
     CDS             complement(2788..2952)
                     /locus_tag="OO590_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCR6638510.1"
                     /translation="MRGLAYNLGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRNGKK"
     gene            complement(2963..3256)
                     /locus_tag="OO590_003245"
     CDS             complement(2963..3256)
                     /locus_tag="OO590_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCR6638511.1"
                     /translation="MPAPQYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(3270..3776)
                     /gene="ssb"
                     /locus_tag="OO590_003246"
     CDS             complement(3270..3776)
                     /gene="ssb"
                     /locus_tag="OO590_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6638512.1"
                     /translation="MASRGVNKVIILGRVGQDPEVRYSPSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQSGQDRYTTEV
                     HVGINGVMQMLGGIGDSKQQAASRQSQKPQQQSSPAQHNEPPMDFDDDIPFAPVTLPF
                     PRHAIHAI"
     gene            complement(3777..4484)
                     /locus_tag="OO590_003247"
     CDS             complement(3777..4484)
                     /locus_tag="OO590_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR6638513.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIRIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKHGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            complement(4739..4891)
                     /gene="kil"
                     /locus_tag="OO590_003248"
     CDS             complement(4739..4891)
                     /gene="kil"
                     /locus_tag="OO590_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCR6638514.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(4876..5007)
                     /locus_tag="OO590_003249"
     CDS             complement(4876..5007)
                     /locus_tag="OO590_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HCR6638515.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVDVLLCAMK"
     gene            complement(5032..6000)
                     /locus_tag="OO590_003250"
     CDS             complement(5032..6000)
                     /locus_tag="OO590_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HCR6638516.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVHDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAKRERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            complement(6168..6410)
                     /locus_tag="OO590_003251"
     CDS             complement(6168..6410)
                     /locus_tag="OO590_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638517.1"
                     /translation="MRVKTMGASLLSGRIFQGTLNTEKGMWVGKREDVTDQAVRAVAE
                     HMMIKDQKYAYETKDGKWLIISHQLVDKLPEEFVDG"
     gene            complement(6613..6945)
                     /locus_tag="OO590_003252"
     CDS             complement(6613..6945)
                     /locus_tag="OO590_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6638518.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRTERKNKRTWYSKPRSEIGVTCSGRQKQRG
                     KSIPAYYD"
     gene            complement(7406..7810)
                     /locus_tag="OO590_003253"
     CDS             complement(7406..7810)
                     /locus_tag="OO590_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638519.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGSMNGDRYEFTKDST
                     EDSVFHVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            complement(7807..8439)
                     /locus_tag="OO590_003254"
     CDS             complement(7807..8439)
                     /locus_tag="OO590_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR6638520.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            8543..8758
                     /locus_tag="OO590_003255"
     CDS             8543..8758
                     /locus_tag="OO590_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6638521.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            8878..9171
                     /locus_tag="OO590_003256"
     CDS             8878..9171
                     /locus_tag="OO590_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HCR6638522.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            9204..9350
                     /locus_tag="OO590_003257"
     CDS             9204..9350
                     /locus_tag="OO590_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HCR6638523.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            9343..10176
                     /locus_tag="OO590_003258"
     CDS             9343..10176
                     /locus_tag="OO590_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HCR6638524.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVHLILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKF
                     KKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTDKEYNNIISNTDVLESTAA
                     DKKSDKKKPSVSCQDVVDAYHEILPEAPKIRALNDKRKNQIRTFWRKAGVITRQLDGH
                     GFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGFDFLLNDNTYLKVREGEHDD
                     R"
     gene            10166..11602
                     /locus_tag="OO590_003259"
     CDS             10166..11602
                     /locus_tag="OO590_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6638525.1"
                     /translation="MTDNFYAPPHSIEAEQAMIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKIIFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPSETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            11679..12119
                     /locus_tag="OO590_003260"
     CDS             11679..12119
                     /locus_tag="OO590_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR6638526.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            12098..>12173
                     /locus_tag="OO590_003261"
     CDS             12098..>12173
                     /locus_tag="OO590_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR6638527.1"
                     /translation="MGRQGGMRRQRRSFTDIICENCKYL"
BASE COUNT         2945 a         2990 c         2712 g         3526 t
ORIGIN      
        1 ctttttcagt ccacatgcga ttgaggcaag agttgcgcta ctgatgccaa catcgccagt
       61 cgattgccat ttcaaaacct tcatagccaa atctgacatt tcttgtctcc aataaaaaac
      121 cgccatcagg cggcatggtg ttctttcagt tcctcaattc gaatattggt tacgtctgca
      181 tgtgctatct gcgcccatat catccagtgg ttatagcagt cgttgatgtc ctctgcttcg
      241 ataaccctgt cgaatggctc tccattccat tcacctgtga ctcggaagtg catttatcat
      301 ctccataaaa caaaacccgc cgtagcgagt tcagataaaa gaaatccccg cgagtgcgag
      361 gattgttagt tgctatctgc tgcttccttg gtcattacca tatccaccca atttcaccag
      421 acatgattct cgcaatcact attatcacca aagtgataat cacaacttta actggcggca
      481 tcattcacca tcctgctccg gcggttctag cagcggcatc cagtgggtta cgtcatccaa
      541 gatatttcct gataaatatg tgaaagctct atattttttg taatcaattg gactcattac
      601 ccagttccaa tatgctgcca ctatttgacc ttgactaaat gccagtaaca ttttggtgtc
      661 ttccggcatt cgctcactac agcttatcca accatccgga gttaccggat agttgcgcat
      721 tgcgaccttt aatgcctcat agaagcaacc tttcagattg ttgaactgac gcccatttag
      781 cgggccatgt tcagtcagca tgttgtgtaa tttccatgcc gcgtcgttta cttcgttgga
      841 tgacagggga ggcaacttgt aagtttggct tacaggttcg gcaccatgaa gcatggcgtc
      901 gctccgctct atgccatcca gcgcgattcg tagtgcctga attgtggtag agctatcgtt
      961 tggggctatt ccatatcgct cgaatacagc taaatggttg cgcataatct caggcgtaag
     1021 ctctttgtaa gcataagcga gaggctctga tgcattatcc ggcacaaccg acgcaggcgc
     1081 ggcagcataa acaggaataa cgtccgcttg ctctttattg ctttcatccg ttaaagccca
     1141 gaataatttc ccggccggat gtttgaaaat ataagcaact ggctctgctt ccagcgatgc
     1201 cagtgcaatt cgtgccagtt cttccgcttc ttctgctggc agtacaacgt tgctaccagg
     1261 tccgtatgtt tcgcgccact gcttgattgt cagtagtcgc tctttggtaa tagtggtcat
     1321 ctcactctcc tttgatgcga ataccagtag cgcggattgc atcgatgact tcagaaactt
     1381 tgtatgccat taccgtttgg taatcctcgt gaaaatctgt tcgatgaagc atgctgctac
     1441 gttccgggag cagtatttcc cgctcctcca gttctgctat gcgcttctct gcggattcca
     1501 gcttctcgcg catatcgtca acgtactcga ccagagaacc gccagcagga atttcgcact
     1561 cctcgaccag ttggaagtag atatcagctg cggcccgtgt gttgctatac ctagcgtcgc
     1621 ccatctcacc ttcacgaaga gcatcgcgtt cggcggtaag attggctatt ttgctgtctt
     1681 tgccttccag ctcaacgcgc agcttcccta ccgtaagcgc aatatcctcg ttctcctggt
     1741 cgcggcgttt gatgtattgc tggttccttt cccgttcatc cagtagtgcc tgcgctactt
     1801 cagggttgaa agctgcgata taacgagcgt tgttctctgc gttgttctgt ccatcaaagc
     1861 cggtccattt gataacgtct tcacatcgtt tatcaccagg tgtatgcacc gcatatgtac
     1921 cagtaccaga cgaaataaat gcgacccatt caccctgtgt tgccagttgc gccgctttac
     1981 gcagtgcctg gtaattaatt tcgctcactg gttgcctcct ttgctgggct ttctaacttt
     2041 tgagtggttg tatcaaattc aaacaactta accacgtcat caaacaggac ataatcacca
     2101 tcagaatctt cagtcatgtc agcgccacaa tcctgaccgc atgagtcaca accacccata
     2161 tcaagctcgt atcgcttcag gtttgcgata tttgataaat tcagcgccag tacagccagg
     2221 tcataaacct cttcggcagt gacatcgctg ctcagtccca tttcatggcg atatatgatt
     2281 ttttctactc gttgttttgt gattgtcatt tttgctcgcc tccctgttct tccagaaaaa
     2341 tacgcatagt ctcaagcatc tcttcggtgt catacggtga caacttgtca cgcaggatgt
     2401 gttcaatgct gttaatgaac ttgcggattg ctttgcgttc aatttcagcc agaaaagcat
     2461 cggttgccgg ggtttcaggt ttaatggctt caagcacggc gcgaatgaca tcggtatcgt
     2521 tctccaccca tgaccattcg cttgtatcat tccagtcgtg gtctatgata gctgtttcat
     2581 tgaatgcctc gactgccgta tccgggatta cttcatgatt taaagctgcc ttcagccccg
     2641 cattctccgc cgccagcgcc gcgcacttgg cctccgcttc agcaaattta cgcaccagat
     2701 attcagcgtt tgtttcgtta acctttaaat cacttgggat gcatttacct ttcagaaatc
     2761 catccatctc aattagtgtc atttgtttca tttcttccca ttccgccaca ttgcattcag
     2821 atatttgttt tgatttactg acggaaaaga atttctttta agcaattcct ctctcgatgg
     2881 cattggcttt acgcgttggc gaataatcat ttctgccgga agaatgccga gattgtatgc
     2941 aagtcctctc atggtaaatt cctcagtcat tactgatagc gccatagcgt gagcggtaat
     3001 tacgcaggcg cgggtcaatt tcagggaagt gggtatatgt ggctttgcgg aatggtcgga
     3061 ttgatgtctg gtaaattcgc tcgcgctctt ctttctctgc aagccatatg cagtggcgaa
     3121 attccttttc ctctttcgtt tcctgcggta gcgacattat ccggtcgtag tttttcctga
     3181 atttatccag cacctccgat acggaattgc cggaacagcg gcgcgggtca tccgcaccat
     3241 actgaggcgc tggcatgatt ttctcctgat taaattgcgt gaatagcgtg acgagggaag
     3301 gggagagtta ctggtgcaaa gggtatatcg tcgtcaaaat ccatcggagg ttcgttgtgt
     3361 tgtgctggtg atgattgctg ctgtggcttc tgtgattgcc tgctggctgc ttgttgtttg
     3421 ctgtcgccaa tgccgccaag catttgcatc acgccattaa ttccgacatg aacctcggtt
     3481 gtgtaacggt cttgccctga ctggtctttc cactttctgg ttctcagcat tccctcgaaa
     3541 taaatctgat cacctttttt cacatactgc cccacgacct cagccagttt cccggataca
     3601 gcaacacgat gccattcagt caattccttt tgctcgccag tatttttatc tcgccattgt
     3661 tctgacgtgg ctattgtcag gttagcgaac gctgttcctg atggtgagta tcgaacttcc
     3721 gggtcttgtc ctacccgacc aaggataatc accttattta cgcctctgct tgccatttat
     3781 gccgcctgtt ttagttcgtt aactctgatg ttcattacct gaacgcattt agcctgcgct
     3841 tcctcgttgc cagccattaa ttgccagtca cgctgataac gctcgatgag ttttttcttg
     3901 tcagtttctg ttgacgcata atcgctgaag tctttcagga tttgttcgca gtcaaccgat
     3961 ggagatttct ggttggtatt ttctggtgat ggtttgttat ctgatgctgg gattgcccat
     4021 cccggcagcg atggagggag ccagtaaaat cctgttccat ccttcagttt tgccctgtgc
     4081 cacccatgct ttttatcgag agatgtttgt gcgaaacctt cctcaaggtt atacagatac
     4141 cgaccgattc cccactgaac ggcagcacgc ttcattgcac ctgaacgacc acctttgacg
     4201 gcttctacct gcgtgttttc agcagcatcc catttggtta cccattcgga atcaatcctg
     4261 attgatatgc cgcattcaac gcctccgttg ttgggaatat cgcggtattc attgcgccat
     4321 cctgctttgc cgcaaacatc gtccaggcgt ttcatgattg cccggttcgt gacataagcc
     4381 agcaccatag cccacacttt gccatcgcgt gttttaccgc tttgctgtat tcgccattcg
     4441 atatcttcag ggctgaatgg ctcatcgaat ttattcaaat ccataaatca cctcagaatg
     4501 gacatggccc aagaaaataa cgctgattta atacttcgac tcgggacaaa ttaaggcata
     4561 cccgcattcc ttcgcggtcg ccattatggc gataccagag agctttctgg gtgtacatgc
     4621 gtctctgtaa cttgctctcc ttcactgtgg ttgcaagtga catgaatatc tccttcgttt
     4681 ccgattaatt ctttcatctg acgaatgaat tcttcgtctg accagttatc tgtaaaactc
     4741 atttcctgcg ataccacgga aggttgatag ctgacttcat cgctttattt gcttcaagcc
     4801 acattttgga atcaccaata aatctggcta ttactgcttt gttctgtgca gcacgaagca
     4861 tctggtgatt gatggctatt tcattgcgca taataagaca tcaactcttt tccatccgtc
     4921 acgtaattta cgggtgattc gttcaagtaa agattcattt aattggaagg cacccatgcg
     4981 agcgcctccc gcgattgcgt aaatcatggg tggttcctta tgttggtttt attagtaggt
     5041 tatttttgtt gcgaatactt cgccttttac gatggctgtt atgatatttt tagcaacatc
     5101 ttctgatgcg ccaaccttga taaggtcagc aagtattttg ttatttactt cttttcggtg
     5161 agctttatcc tttgctctac gctcttcttc gtccttgatt cttttttctt ctgctattct
     5221 ggcttgctct tttgcttcag cttcgcgccg gattcgttca gcctcctcct gtgcttttcg
     5281 gcgttctgct tcaattgccg cctgcttttc tctttcagct cgttctgctg cctcttttgc
     5341 ttcgcgctgt gctcgttgct cggcttcaat gcgttcacgc tttgcacgtt ccgctgcggc
     5401 cttagcttct gcttctcgcc ttgctgctgc ttcaatttcg gcttttgcct ttgcttcggc
     5461 ttctgctctg gctttctctt cagcttctct ttttaagcgt tcttcatgct ctcgcttttc
     5521 ctgctccgct ttgagtcttg cctcttctct ttggcggtca aattcgcgat ccatcaaaat
     5581 cgctatttca tggtcagact caatttgctt tgcgagagct tcagctgctg ccttagcttc
     5641 ttcttcggct ttaatccgcg cctgttcctc ctcataatca gtaagaggct ggcgcgcctt
     5701 ggctttcagc tcatcaagtc gatcatgcac tgttttgcgg ttggcatcaa ttagctttgg
     5761 aatttccttc agttcagcaa caaggtcttt gccaagacca tcgagatatg ttttcgtctg
     5821 cgcaacttta tacgccagag aagcgatctc ctttctgccc tttgccgttg tgatatcagg
     5881 cacaaaggac ataacttcac gttcaacctt ttgaaggatt tcttcaatct ggtcggcaga
     5941 ctgaaataca gtcattgcat ttgctttttc aataacaact aaatctgtta cttcactcat
     6001 atttcccccg tcaaaaaaat tgccctcaca ctggagggca aagaagattt ccaataatca
     6061 gaacaagtcg gctcctgttt agttacgagc gacattgctc cgtgtattca ctcgttggaa
     6121 tgaatacaca gtgcagtgtt tattctgttg cttattccaa agcaaattta accatcaaca
     6181 aactcttctg gtaatttatc aaccagttga tgacttatta tcagccattt gccatccttc
     6241 gtttcgtatg catatttctg gtcttttatc atcatgtgtt cggctactgc cctcactgcc
     6301 tgatcggtga catcttctct ctttcctacc cacattcctt tttcagtgtt taatgttcct
     6361 tgaaaaatac gaccacttaa taggcttgcg cccatagttt ttactctcat gtatcagtcc
     6421 tcaaataagt ggtttgctgc gaaagtaaat acgcttaagt tacctgttat ttatcccacc
     6481 aagttccgta tctatctatc cagttacacc aatcatcgac actctatttt gttgtgtcgc
     6541 attttggcaa ctggcaagaa tatctacctt ctttgtaaag tcggcgtttg actttcttga
     6601 gcatggctca cctcaatcgt aataagctgg aattgatttt ccgcgttgct tctggcggcc
     6661 tgagcaagtc acacctattt cactgcgtgg cttgctgtac catgtgcgct tattcttgcg
     6721 ctcagtacgc tgcaggttgc tttcaatctg ttcgtggtat tcagccagca ccgtaaggtc
     6781 tatcggattc agtgcgcttt ctactcgtga tttcggtttg cgattcagcg agagaatagg
     6841 gcggttaact ggttttgcgc ttaccccaac taacagggga tttgctgctt tccattgagc
     6901 ctgtttctct gcgcgacgtt cgcggcggcg tgtttgtgca tccatctgga ttctcctgtc
     6961 agttagcttt ggtggtgtgg tggctggtag tctagctcca gcttgttgag tctcattcgg
     7021 aggggtataa ctggcacccc agcgattttt ccatgcgaca acgtgcgcgt tatggcggcc
     7081 ttatcgcccg cggctcccca tctcgtccac gctattgcta gcgttgggag cgcttcaccg
     7141 ctcaacagta ggtaagcact tgccagtgac tagctggctt caccacaccc caaagccttc
     7201 tgctttgaat gctgcccttc ttcagggctt attttttaag agcctcacct tcatggtggt
     7261 cagtgcgtcc tgctgatggc ttaaaattac aagaaagatt gtatgttgta aacaagaaat
     7321 attgtgaaaa ggggcgtgaa aaacaaactc cattgttttt aaatgaaaaa tagtttgttt
     7381 ttggttatcg agattgaggt ggggattact ggttgcaggt tccgactaca tcaccaacaa
     7441 atgatttggt tgatgtaagt tgttgcatac ctgggatatt cattactttg gagtaaagag
     7501 cttttttgtc tgtagtgatt gaccaagttt caacggttat tcctcctcca gactggtatt
     7561 ctcctaccat agtgttcgat gacaaagcag tgtatttcat ctctggatag acgccagaaa
     7621 ctgattcata aactgatgat ttatcgccat ttattgttac gtggaaaacg gaatcttccg
     7681 tgctgtcttt tgtaaactcg taacgatcgc cattcattga cccgtacccg tgcaggtttg
     7741 tgacaatcca gcattcagaa ttggcgctgg tagttaagag tattgagagt agcgccgcaa
     7801 tcctgatcat acgaatttta ccctcgcttc cacgacaaca ccgataatct tgcagttccc
     7861 gttgatagga gtcataggcc atgaagggtt caggcctttc aggtacttct gcccgccatc
     7921 tataaccagt ttcttgaatg ttgcttcgtt cgcgtcagtc agtttggcta caacaaggct
     7981 tccattcact ggctcgcgtc cagtatctac taacaccata tgaccttcag ggatgctttg
     8041 acctacaggt gaggtcatgg aatcaccttc aaccttcagc cagaatccat cgcctaataa
     8101 gttaacgtca ctgtcatacc attcatcaat gtccttgata tcgtagggtt cacaagcttc
     8161 acaccacgaa ccagctctaa ccatgctaat caatggatat ttccctttgg gttcaacatg
     8221 cccaacaaat ctaacattcg aatcagaggt gccattgagc agccagtcaa cacttacgcc
     8281 aagagctgac gcaagttctg gtaaaaagcg tggtcgctta gttttaccgt tttcgagctg
     8341 ctctatagac tgctgggtag tccccacctt ttgagcaagt tcagcctggt taagtccaag
     8401 ctgaattctt ttgcttttta ccctggaaga aatactcata agccacctct gttatttacc
     8461 tccaatcttc acaagaaaaa ctgtatttta caaacaagat acattgtatg aaaatacaag
     8521 aaagtttgtt gatggaggcg atatgcaaac tctttctgaa cgcctcaaga agaggcgaat
     8581 tgcgttaaaa atgacgcaaa ccgaactggc aaccaaagcc ggtgttaaac agcaatcaat
     8641 tcaactgatt gaagctggag taaccaagcg accgcgcttc ttgtttgaga ttgctatggc
     8701 gcttaactgt gatccggttt ggttacagta cggaactaaa cgcggaaaag ccgcttaaga
     8761 cattcccgct cttacacatc ccagtcctga aaaagggcat caaattaaac cacacctatg
     8821 gtgtatgcat ttatttgcat accttcaatc aattgttatc taaggaaata cttacatatg
     8881 gttcgtgcaa acaaacgcaa cgaggctcta cgaatcgaga gtgcgttgct taacaaaatc
     8941 gcaatgcttg gaactgagaa gacagcggaa gctgtgggag ttgataagtc gcagatcagc
     9001 aggtggaaga gggactggat tccaaagttc tcaatgctgc ttgctgttct tgaatggggt
     9061 gtcgttgacg acgacatggc tcgattggca cgacaagttg cttcgattct caccaataaa
     9121 aaacgcccgg ctgcaaccga gcgttctgaa caaatccaga tggaattctg aggtcattac
     9181 tggatcaatc cacaggagta attatgacaa aacaactcag tccttaccag gacaaaattc
     9241 acaaacacat actacgtgat cgcttcctgt ccagcttcaa gcagcctggt cgattccggg
     9301 ctgagttgga aaaagtgaag ctgatgcaga aggagaaagg tcatgagtaa catatctaat
     9361 ctagccgaag ccagagaggc cagaaggcta caacaaccgc atcaaagcag cggtaagggg
     9421 tatgccttgc tgcaccgtaa aattatggat gtgccgtttt acaaggacgc agaagcagcg
     9481 catctgtggg ttcacttaat cctcaaagca aagcatacgc ctgagtatgt aatgactgac
     9541 gcaggagaaa ttctggtagg cagagggaag ctacttggcg gtagaaactc tctggcgttt
     9601 gaaacaggac tcaaaccaga tcgcgttcag tacctgctta gaaagttcaa aaaactcggc
     9661 atgattgact gggtttcaca cggtaaattc tcagttttct cggtagagaa atatgacgat
     9721 tatcagtcaa attttgtacc agcagattac cagcaaatta ccacagataa agaatataat
     9781 aatattatct ctaatactga cgtattagag agtaccgcag cagacaaaaa gtctgacaag
     9841 aaaaaacctt ccgttagctg tcaggatgtt gtcgatgctt accacgaaat ccttcctgaa
     9901 gcgccaaaaa tccgcgcact gaatgacaag cgtaaaaacc agatccgaac gttctggcgc
     9961 aaagccggag tgataacccg ccagcttgac gggcatgggt tcacgatgca ggactggaga
    10021 aattatttga gctacgtagg cgaaaattgc cgatggatgt tcgaagagcg cccaaaccat
    10081 cagcgcggaa ccgtctggca caaaaaggga tttgatttcc tgcttaacga taatacctac
    10141 ctgaaagttc gtgagggtga acacgatgac cgataatttt tatgcgccgc cccatagcat
    10201 cgaggcagag caggcgatga ttggtggatt gcttctggat gatgacagca gtgagcgcgt
    10261 ccagaaggtt ctggcgatgc tgaagcctga ttcattttac agccgaccac acaaaatcat
    10321 tttcgaagaa ataaccagaa tgcaccggga gcaaaagcca gtagatggcc tgacgctttt
    10381 cgatgaactg gagcgtaaat cgttaacggc gtctgttggc ggttttgctt atatcgctga
    10441 gatcgcaaag aacacgccaa gcgccgcaaa catcgttgcc tatgcaatgc aggttcgcga
    10501 aaccgcaatg gaacgctacg ccatcaaccg catgactgaa gcgacggaat tgctctattc
    10561 ccgcaacgga atgactgcga cgcagaagta cgaagctatt caggcgattt tcacgcaact
    10621 gacagaccat gcaaaaacag gatcgcgtcg cggccttcgc tcatttggtg aggtcatgga
    10681 agactgggtt agcgaccttg agaagcgatt tgacccatca ggcgaacaac ggggaatgag
    10741 cacagggatc ccatcgctgg acaggatgct gtcaccgaaa ggtctggtga aaggctctct
    10801 gtttgtcatt ggcgctcgac ctaagatggg gaaaacgacg ctatacagcc agatggcaat
    10861 caactgcgca gtgcatgaga aaaagcccgc tcttatgttc agccttgaaa tgccaggtga
    10921 ccagatactg gaaaaactgg tagggcagaa gtctggtgtt aacccgaata ttttttacct
    10981 tccggcgaca aatgacgctg atgacggcta tcagggtgat tacgatggtg acttcaacag
    11041 ggcgatcgaa acagccaatc gcttgagtga aatcgacctg ctttacatcg acgacacgcc
    11101 gggattatct ctggctcaaa tcgtcagcga aagccgtcga atcaagcgag aaaaaggatg
    11161 tgttggcatg attctggtcg attacctgac actaatgacc gctgaaaagg ccgatcgcaa
    11221 cgaccttgct tacggcatga tcaccaaagg actgaagaac cttgccaaag agcttgattg
    11281 cgttgttgtg cttctgacac agcttaaccg cgcactggaa agccgaacca ataaacgccc
    11341 attaccaagt gactcacgag atacagggca gattgaacag gattgcgatt attgggtggg
    11401 gatccatcgt gaaggtgctt ttgatgacag tgttccacct agtgaaaccg aactaatcct
    11461 tcgtctcaat cgtcatggca ataccggcac ggtgtattgc attcaggcaa atggcgctat
    11521 ttatgacaca gaccaacagt ctgctgaaat gcgccgacgt gaacgcgagg aaccgcagtc
    11581 caagaagaaa ggaggattct gatgaccatc tacatcactg agctaataac aggtctgctg
    11641 gtaatcgcag gcctttttat ttgggggaga gggaagtcat gaaaaagcta acctttgaaa
    11701 ttcgatctcc agcacatcag caaaacgcta ttcacgcagt acagcaaatc cttccagacc
    11761 caaccaaacc aatcgtagta accattcagg aacgcaaccg cagcttagac cagaatcgaa
    11821 agctttgggc ttgccttggt gacgtctctc gtcaggttga atggcatggt cgctggctgg
    11881 atgcagaaag ctggaagtgt gtgtttaccg cagccttaaa gcagcaggac gttgttccta
    11941 accttgccgg gaatggcttt gtggtaatag gccagtcaac cagcaggatg cgagtaagcg
    12001 aatttgcgga gctattagag cttatacagg cattcggtac agaacgtggc gttaagtggt
    12061 cagacgaagc gcgactggct ctggagtgga aagcaagatg gggagacagg gcggcatgag
    12121 gcgacagcga cgaagtttca ccgacatcat ctgcgaaaac tgcaaatacc ttc
//
LOCUS       DAJTYF010000112        12141 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTYF010000112 DAJTYF010000000
VERSION     DAJTYF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.112"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            115..984
                     /locus_tag="OO590_003262"
     CDS             115..984
                     /locus_tag="OO590_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR6638528.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALPLKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYARTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1187..1540)
                     /locus_tag="OO590_003263"
     CDS             complement(1187..1540)
                     /locus_tag="OO590_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6638529.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1678..3324)
                     /gene="groL"
                     /locus_tag="OO590_003264"
     CDS             complement(1678..3324)
                     /gene="groL"
                     /locus_tag="OO590_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6638530.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3368..3661)
                     /locus_tag="OO590_003265"
     CDS             complement(3368..3661)
                     /locus_tag="OO590_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6638531.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3937..5193
                     /gene="yjeH"
                     /locus_tag="OO590_003266"
     CDS             3937..5193
                     /gene="yjeH"
                     /locus_tag="OO590_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6638532.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5209..5685)
                     /gene="fxsA"
                     /locus_tag="OO590_003267"
     CDS             complement(5209..5685)
                     /gene="fxsA"
                     /locus_tag="OO590_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6638533.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6022..7458
                     /gene="aspA"
                     /locus_tag="OO590_003268"
     CDS             6022..7458
                     /gene="aspA"
                     /locus_tag="OO590_003268"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6638534.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7576..8877
                     /gene="dcuA"
                     /locus_tag="OO590_003269"
     CDS             7576..8877
                     /gene="dcuA"
                     /locus_tag="OO590_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6638535.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8993..9331
                     /gene="cutA"
                     /locus_tag="OO590_003270"
     CDS             8993..9331
                     /gene="cutA"
                     /locus_tag="OO590_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6638536.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9307..11004
                     /gene="dsbD"
                     /locus_tag="OO590_003271"
     CDS             9307..11004
                     /gene="dsbD"
                     /locus_tag="OO590_003271"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6638537.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11041..11616
                     /locus_tag="OO590_003272"
     CDS             11041..11616
                     /locus_tag="OO590_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6638538.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11723..11798
                     /locus_tag="OO590_003273"
     tRNA            11723..11798
                     /locus_tag="OO590_003273"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11756..11758,aa:Phe,seq:gaa)
BASE COUNT         2924 a         3136 c         2986 g         3095 t
ORIGIN      
        1 tagagtgagc acggctattt atgttatttt aacaactatt actaccaagc ccatttatct
       61 ggcttataaa ttaaaattaa ttatttaatt gagaaatggt tagggagaac ctacatggcc
      121 ataagcatca agggtgttaa taccggtgtt attcgcaaga gtaataattt cattgcactg
      181 ccgctgaaaa ttaaagaacc tcgcaataaa gaatcgttgt tctttatgtc tgtcatggaa
      241 ctgcgcgacc tgctgattgc gctggaaagt cgtctgcatc aaaagcataa actggatgct
      301 gctgcacgcc ttcagtatga gcaagctcgc gataaagtca ttaagaaaat ggcagaaaac
      361 atcccagaaa ttctggttga tgagcttaaa aatgccgata ttaatcgtcg ggttaacacg
      421 ctggaactta ccgataatca aggcgaaaat cttaccttcg tattaacgct acatgacggt
      481 agcaaatgtg agttagtcgt caatgagttg cagattgaaa tgctggcgcg agcaatcatt
      541 catgcaatta ataatgcgga aatgcgtgaa ctggcattac gtattacctc actgttagat
      601 ttcctgccgt tatacgacgt tgactgccag gacaatggca atctggaata tgacacctat
      661 tcccaaccgg aatggaaaca taacttgttc gaccattatc tggcggtgct ttaccgcttt
      721 aaagatgaaa gtggcaaaga acaatttagc ggcgcagtgg tgaaaacgcg tgaagcaacg
      781 ccgggtaaag aaatagaggc gatcactcgc cgaatgcttg atttcagccc acgactgaaa
      841 aaactcgcgg gtgtgccatg tcaggtctat gctcgcaccg tagcggcgaa taacgcccaa
      901 ccgctaaccc aggatcaatg cttacgcgcg ctgcatcact tacgtgtaca atccaccagc
      961 aaaactgcac cacaggcgaa ataatctcat ctttgctaat gcgaggctgg agccatccag
     1021 cctgaaatga ctgataacgt gatcgttggt catgccggat gcggcgtaaa cgccttatcc
     1081 ggcctacaaa ttctcaagca ttcaatcagt tgcaatgcct gctgtaggcc tgataagcga
     1141 agcgcatcag gcaataatac gtttgtcacc cagcctctgg atcacatcag ttcgggcact
     1201 tataaacctg accgataatc tggctatccg tcgggacaaa actggacaac attccctgcg
     1261 acgggctgct gatgccataa atcacgttac cgcccattgc agccgcctgg ttgcgcagat
     1321 cgtttgctgc gccgcgcata gaaccgccct cttctccgtg ttgcccggaa agccagttgc
     1381 tttgcttacc tgtcgcagta ccaatcagct ggcactctgc gcctggctgc tcgtccacaa
     1441 tgcgtacact ctgaccggca gcactcaatt cgttgctgga gctacaaccc gccatcagta
     1501 gcgcggcacc gacaatccct gctaagtatt ttacgtgcat gttattcccc ataatcaatg
     1561 agctggacgc cactcgcgtc gtccgtgtct gaatcttata ctagaaagat gaccaaaaga
     1621 aaaaccccca gacatttctg cccgggggtt tgtttatttc tgcgaagtgc agggcaatta
     1681 catcatgccg cccatgccac ccatgccgcc cataccgcca gcagcgccta agtcagctgc
     1741 atcgtttttc ggcaggtcgg taaccatgca ttcggtggtg atcatcaggc cagccacaga
     1801 agctgcgtac tgcagagcag aacgggttac tttggttggg tccaggatac ccatgtcgat
     1861 catgttgccg tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac
     1921 ggtgttagca acaacagacg gttcttcgcc gcagttcagg acgatctgac gcagcggagc
     1981 ttccattgca cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct gaccacgcag
     2041 gtcagccagt ttagacgcta cgcggatcag cgcaacacca ccaccagcaa ccacgccttc
     2101 ttctaccgca gcacgggtcg cgtgcagggc atcttcaacg cgtgcttttt tctctttcat
     2161 ttcaacttcg gtagcagcac ctactttgat aactgcaacg ccgcctgcca gtttcgctac
     2221 gcgctcctgc agtttttcac ggtcgtagtc agaagttgct tcttcaatct gctgacggat
     2281 ctgagcaaca cggccctgga ttgcagcttc ttcacccacg ccatcgatga tagtggtggt
     2341 gtctttgttg atcacaacac gtttagcctg acccaggtct tccagggttg ctttttccag
     2401 ctccataccg atctcttcag agattacggt accgccagtc agggttgcga tatcctgcag
     2461 catagcttta cgacgatcgc cgaagcccgg agctttaacc gcagcaactt tcacgatgcc
     2521 acgcatggtg ttaacaacca gagttgctag cgcttcgcct tcaacatctt cagcgatgat
     2581 cagcagcggt ttgcctgctt tggcaacagc ttccagaacc ggcagcattt cgcggatgtt
     2641 ggagattttc ttgtcagcca gcaggatgaa cgggctttcc agttctactg cgccagtttc
     2701 cggcttgttg atgaagtaag gagacaggta gccacggtcg aactgcatac cttcaaccac
     2761 gtccagttcg tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac
     2821 tttgtccatc gcttcagcga tcagtttacc tacggtttcg tcggagttag cggagatggt
     2881 accaacctga gcaatcgctt tagaatcaga gcacggtacg gacagcgctt tcagttcttc
     2941 aactgcagcg gtaaccgctt tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc
     3001 agcaacagct ttcagacctt cagtgatgat agcctgagcc agtacggttg cagtggtggt
     3061 accgtcgcct gcagcgtcgt tcgctttgga ggcaacttct ttcaccatct gcgcacccat
     3121 attttcgaac ttgtcttcca gttcgatttc acgagcaacg gaaacaccat ctttggtgat
     3181 ggtcggtgca ccgaaagatt tatccagaac tacgttacgg ccttttggac cgagggtaac
     3241 tttcactgca tctgccagta cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc
     3301 gaattttacg tctttagctg ccattatctt tattccttaa attcgtatgt tcagtgtcgt
     3361 gcgcggatta cgcttcaaca attgccagaa tgtcgctttc ggacatgatc aacacttctt
     3421 cattgtcgat cttctcggat ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa
     3481 ctttcacgtc cagcggcttc acttcgccat tttcaaggat acggccattg ccgacagcca
     3541 gcacttcgcc gcgggtggat ttagccgctg cagagccggt cagaacgatg ccgccagcag
     3601 atttagtttc aacttcttta cgcttgacga tcacgcgatc atgcaatgga cgaatattca
     3661 ttgataactc tcctttgaga aagtccgtat ctgttatggg tgacgccgga gcttacgtgg
     3721 tttcccggct ggtgaccaga gaaatgggga tgaggcttcg ccccttcaag ggggaaaaag
     3781 aaaaaaaatt ctgaacagca caaaatcggg tgaaaaccct gattcacctc acatttcatc
     3841 gcaatttctt catcgccgtc ataagcgaat ctgattgtgc taccatcgaa aatctacgca
     3901 gttgcccaaa atttgggcgc aatcggatat caacccatga gtggactcaa acaagaactg
     3961 gggctggccc agggcattgg cctgctatcg acgtcattat taggcactgg cgtgtttgcc
     4021 gttcctgcgt tagctgcact ggtagcaggc aataacagcc tgtgggcgtg gcccgttttg
     4081 attatcttag tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca ctatcccagc
     4141 gcaggcggcg tcgcgcactt cgtcggtatg gcgtttggtt cgcggcttga gcgagtcacc
     4201 ggctggctgt ttttatcggt cattcccgtg ggtttgcctg ccgcactaca aattgccgcc
     4261 ggattcggcc aggcaatgtt tggctggcat agctggcaac tgttgttggc agaactcggt
     4321 acgctggcgt tggtgtggta tatcggtact ctcggtgcca gttccagtgc taatctacaa
     4381 accgttattg ccggacttat cgtcgcactg attgtcgcta tctggtgggc gggcgatatc
     4441 aaacctgcga gtatcccctt ccccgcgcca ggaaatatcg aacttaccgg gttatttgct
     4501 gcgttatcag tgatgttctg gtgttttgtc ggtctggaag catttgccca tcttgcctcg
     4561 gaatttaaaa atccagagcg tgattttcct cgtgctttga tgattggtct gctgctggca
     4621 ggattagtct actggggctg tacggtagtc gtcttacact tcgacgccta tggtgaacaa
     4681 atggcggcgg cagcatcgct tccaaaaatt gtagtgcagt tgttcggtgt aggagcgtta
     4741 tggattgcct gcgtaattgg ctatctggcc tgctttgcca gtcttaacat ttatatacag
     4801 agcttcgccc gcctggtctg gtcgcaggcg caacataatc ctgaccacta cctggcacgc
     4861 ctctcttctc gccatatccc gaataatgcc ctcaatgcgg tgcttggctg ctgcgtggtg
     4921 agcacgttgg taattcatgc cttagagatc aatctggagg ctcttattat ttatgccaat
     4981 ggcatcttta ttatgattta tctgttatgc atgctggcag gctgtaaatt attgcaaggt
     5041 cgttatcgac tactggcggt ggttggcggg ctgttatgcg ttctgttact ggcaatggtc
     5101 ggctggaaaa gtctctatgc gctgatcatg ctggtggggt tatggctgtt tctgccaaaa
     5161 cgaaaaacgc cggaaaatgg cataaccaca taatccggcg tttcgacatt aatcctggcg
     5221 atcgtcttta tgatcaaggc ggtcgcggtc atcatccttt cgctgatact caccatcaaa
     5281 agtattaccg ccaccggtcc cggcgctaaa accgccgcct ggcatgcgag aaaagcgcaa
     5341 atgtggcatc aacttcactg tcagatgctt ttgcaccggc ggcaataaaa gcagaagacc
     5401 gaggaagtcg gtgaaaaagc ccggtaataa aagcagcaaa ccggcaataa tcagcgaaac
     5461 acttttaatc atctccgccg cagggttttc acccgccgcc attttttgct gcatcagcac
     5521 gaaattctta aagccctggt tacgtaccag tgacataccg ataactgacg tgaatataac
     5581 cagcacgagg gtcagcaata cccccaatac atgggcaacc tgaataaaga ttgaaatctc
     5641 aatatagaca taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt tctcctgtaa
     5701 tagcagccgg ttaaacccgg ctacctgaat gggttgcgaa tcgcgttcag cttatattgt
     5761 ggtcattagc aaaatttcaa gatgtttgcg caactatttt tggtagtaat cccaaagcgg
     5821 tgatctattt cacaaattaa taattaaggg gtaaaaaccg acacttaaag tgatccagat
     5881 tacggtagaa atcctcaagc agcatatgat ctcgggtatt cggtcgatgc aggggataat
     5941 cgtcggtcga aaaacattcg aaaccacata tattctgtgt gtttaaagca aatcattggc
     6001 agcttgaaaa agaaggttca catgtcaaac aacattcgta tcgaagaaga tctgttgggt
     6061 accagggaag ttccagctga tgcctactat ggtgttcaca ctctgagagc gattgaaaac
     6121 ttctatatca gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg tatggtaatg
     6181 gttaaaaaag ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg
     6241 aatgccatca ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc
     6301 ccggtagacg tctaccaggg cggcgcaggt acttccgtaa acatgaacac caacgaagtg
     6361 ctggccaata tcggtctgga actgatgggt caccagaaag gtgaatatca gtacctgaac
     6421 ccgaacgacc atgttaacaa atgtcagtcc actaacgacg cctacccgac cggtttccgt
     6481 atcgcagttt actcttccct gattaagctg gtagatgcga ttaaccaact gcgtgaaggc
     6541 tttgaacgta aggctgtcga attccaggac atcctgaaaa tgggtcgtac ccagctgcag
     6601 gacgcagtac cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa
     6661 gaagtgaaaa acatccaacg taccgctgaa ctgctgctgg aagttaacct tggcgcaaca
     6721 gcaatcggta ctggtctgaa cacgccgaaa gagtactctc cgctggcagt gaaaaaactg
     6781 gctgaagtca ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc gacctctgat
     6841 tgcggcgctt atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa
     6901 atctgtaacg acctgcgctt gctctcttct ggtccacgtg ccggcctgaa cgagatcaac
     6961 ctgccggaac tgcaggcggg ctcttccatc atgccagcta aagtaaaccc ggttgttccg
     7021 gaagtggtta accaggtatg cttcaaagtc atcggtaacg acaccactgt taccatggca
     7081 gcagaagcag gtcagctgca gttgaacgtt atggaaccgg tcattggcca ggccatgttc
     7141 gaatccgttc acattctgac caacgcttgc tacaacctgc tggaaaaatg cattaacggc
     7201 atcactgcta acaaagaagt gtgcgaaggt tacgtttaca actctatcgg tatcgttact
     7261 tacctgaacc cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa
     7321 accggtaaga gtgtacgtga agtcgttctg gaacgcggtc tgttgactga agcggaactt
     7381 gacgatattt tctccgtaca gaatctgatg cacccggctt acaaagcaaa acgctatact
     7441 gatgaaagcg aacagtaatc gtacagggta gtacaaataa aaaaggcacg tcagatgacg
     7501 tgcctttttt cttgtgagca gtaacttaaa aataacaatc taatatcaac ttgttaaaaa
     7561 acaaggaagg ctaatatgct agttgtagaa ctcatcatag ttttgctggc gattttcttg
     7621 ggcgccagat tggggggaat aggtattggt tttgcaggcg gattgggggt gctggttctt
     7681 gccgctattg gcgttaaacc cggtaacatc ccgttcgatg tcatttccat tatcatggcg
     7741 gttatcgccg ctatttctgc catgcaggtt gctggcggtc tggactatct ggttcatcag
     7801 acagaaaagc tgctgcgccg taacccgaaa tacatcacga tcctcgcacc gatcgtgacc
     7861 tatttcctga ctatctttgc tggtactggc aacatctctc tggcgacact gccagttatc
     7921 gctgaagttg cgaaggaaca aggcgttaaa ccttgccgtc cgctctctac tgcagtggta
     7981 tccgcgcaga ttgcgatcac cgcatcgcca atctcagcgg cagtggttta catgtcttcc
     8041 gtgatggaag gtcatggcat cagctacctc catctgctct ccgtggtcat cccgtccacc
     8101 ctgctggcgg ttctggtgat gtccttcctg gtcactatgc tgttcaactc caaactctct
     8161 gacgatccga tttatcgcaa gcgtctggaa gagggcttgg ttgaactgcg cggtgaaaag
     8221 cagattgaaa tcaaatccgg tgcaaaaacg tccgtctggc tgttcctgct gggcgtagtt
     8281 ggcgtggtta tctatgcaat catcaacagc ccaagcatgg gtctggttga aaaaccgctg
     8341 atgaacacca ccaacgcaat cctgatcatc atgctcagcg ttgcaactct gaccaccgtt
     8401 atctgtaaag tcgataccga caacatcctc aactccagca ccttcaaagc aggtatgagc
     8461 gcctgtattt gtatcctggg tgttgcgtgg ctgggcgata ctttcgtttc caacaacatc
     8521 gactggatca aagataccgc tggtgaagtg attcagggtc atccgtggct gctggccgtc
     8581 atcttcttct ttgcttctgc tctgctgtac tctcaggctg caaccgcaaa agcactgatg
     8641 ccgatggctc tggcactgaa cgtttcaccg ctgaccgctg ttgcttcttt cgctgcggtg
     8701 tctggtctgt tcattctgcc gacctacccg acgctggttg ctgcggtaca gatggatgac
     8761 acgggtacta cccgtatcgg taaattcgtc ttcaaccatc cgttcttcat cccgggtact
     8821 ctgggtgttg ccctggccgt ttgcttcggc ttcgtgctgg gtagcttcat gctgtaatga
     8881 tcaatcgcgg ggcgttcacg ccccgctttc tttcccgccg actaacatcc ttcccccgtc
     8941 cgttgtatag tgacctctct cttgcggttc catctgttct tgcgaggtgt ttatgcttga
     9001 tgaaaaaagt tcgaataccg cgtctgtcgt ggtgctatgt accgcaccgg atgaagcgac
     9061 agcccaggat ttagccgcca aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat
     9121 ccccggcgct acctctctct attactggga aggtaagctg gagcaagaat acgaagtgca
     9181 gatgatttta aaaactaccg tatctcacca gcaggcactg ctggaatgcc tgaagtctca
     9241 tcatccatat caaaccccgg aacttctggt tttacctgtt acacacggag acacagatta
     9301 cctctcatgg ctcaacgcat ctttacgctg attctgctac tttgcagcac ttccgttttt
     9361 gccggattat tcgacgcgcc gggacgttca caatttgtcc ccgcggatca agcctttgct
     9421 tttgattttc agcaaaacca acatgacctt aatctgacct ggcagatcaa agacggttac
     9481 tacctctacc gtaaacagat ccgcattacg ccggaacacg cgaaaattgc cgacgtgcag
     9541 ctgccgcaag gcgtctggca tgaagatgag ttttacggca aaagcgagat ttaccgcgat
     9601 cggctgacgc ttcccgtaac catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc
     9661 taccagggct gtgctgatgc cggtttctgt tatccgccag aaaccaaaac cgttccgtta
     9721 agcgaagtgg tcgccaacaa cgcagcgtca cagcctgtgt ctgtttcgca gcaagagcag
     9781 cacaccgcgc aattgccctt ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc
     9841 tttacgccat gcgtgctgcc aatgtaccca ctgatttctg gcatcgtgct gggtggtaaa
     9901 cagcggctct ccactgccag agcattgttg ctgaccttta tttatgtgca ggggatggca
     9961 ctgacttaca cggcgctggg tctggtagtt gccgccgcag ggttacagtt ccaggcggcg
    10021 ctacagcacc catacgtgct cattggcctc gccatcgtct ttaccttgct ggcgatgtca
    10081 atgtttggct tgtttactct acaactcccc tcttcgctgc aaacacgtct cacgttgatg
    10141 agcaatcgcc aacagggcgg ctcacctggc ggtgtgtttg ttatgggggc gattgccgga
    10201 ctgatctgtt caccatgcac caccgcaccg cttagcgcga ttctgctgta tatcgcccaa
    10261 agcgggaaca tgtggctggg cggcggcacg ctttatctct atgcgttggg catgggcctg
    10321 ccgctgatgc taattaccgt ctttggtaac cgcttgctgc cgaaaagcgg cccgtggatg
    10381 gaacaagtca aaaccgcgtt tggttttgtg atcctcgcac tgccggtctt cctgctggag
    10441 cgagtgattg gtgatgtatg gggattacgc ttgtggtcgg cgctgggtgt cgcattcttt
    10501 ggctgggcct ttatcaccag cctacaggct aaacgcggct ggatgcgcgt ggtgcaaata
    10561 atcctgctgg cagcggcatt ggttagcgtg cgcccacttc aggattgggc atttggtgcg
    10621 acgcataccg cgcaaactca gacgcatctc aactttacac aaatcaaaac gatagatgag
    10681 ttaaatcagg cgctcgttga agccaaaggc aaaccggtga tgttagatct ttatgccgac
    10741 tggtgcgtcg cctgtaaaga gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa
    10801 gcgttagcag acacggtctt acttcaggcc aacgtcacgg ccaacgacgc acaagatgtg
    10861 gcgctgttaa agcatcttaa tgtccttggc ctaccgacaa ttctcttttt tgacggacaa
    10921 ggccaggagc atccacaagc acgcgtcacg ggctttatgg atgctgaaac cttcagcgca
    10981 catttgcgcg atcgccaacc gtgaacgaca cttgcagtgg gaaagacgga ggaaaatagc
    11041 gtgcaacgtg aagatgtact gggagaagcc ctgaaattat tagaattaca agggattgcc
    11101 aacaccacgc tggagatggt tgctgaacgt gtggattatc cactggacga gctacgccgc
    11161 ttctggccag acaaagaggc aatcctctac gatgcgctgc gttatcttag ccaacagata
    11221 gatgtctggc gtcgtcagct gatgctggac gaaacgcaaa ccgccgaaca aaagctgctg
    11281 gcacgttatc aggcgttatc ggagtgcgtt aaaaacaacc gctatccggg ctgtctattt
    11341 atcgctgcct gtacgtttta tcccgatcct ggccacccta ttcatcaact ggccgatcag
    11401 caaaaaagcg cggcctacga tttcacccac gaactgttaa ccacgctgga agttgacgat
    11461 ccggcgatgg tagcaaagca gatggaactg gtgctggaag gctgtttaag ccgaatgctg
    11521 gtgaatcgta gccaggcgga tgtcgacact gcacatcggc tggcggaaga tatcctgcgc
    11581 ttcgcccgct gccgccaggg tggtgcattg acctgacaga aacacagaaa agaagcgatt
    11641 tgccgcaatc ttaagcagtt gaatcgcttt tactgaaatt aggttgacga gatgtgcaga
    11701 ttacggttta atgcgccccg ttgcccggat agctcagtcg gtagagcagg ggattgaaaa
    11761 tccccgtgtc cttggttcga ttccgagtcc gggcaccaaa ttcatatcaa cggacctcca
    11821 cggaggtccg tttttcgttt caggacgcca cgatttaagc gttctgcctc caaatcaatt
    11881 ctaccgaact caaccagatt ctccccacat caccagcaat ttgcgagcat atcccaattt
    11941 gggaaaattt gttcctgagc tatagcacta actgacgtga aatgtcgtgc ggccccgtga
    12001 tgctgttgaa cgtcaaatga cgtcatcagg agcgtaacgt atccataaag cacaacaccg
    12061 gtctgaacgc cgactgatga gattttctga atgagaacaa agagaaatgt gtcagtccgc
    12121 ttgctcatgc aaagactaac a
//
LOCUS       DAJTYF010000113        11981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTYF010000113 DAJTYF010000000
VERSION     DAJTYF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.113"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(5..1198)
                     /locus_tag="OO590_003274"
     CDS             complement(5..1198)
                     /locus_tag="OO590_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR6638539.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            1281..2565
                     /locus_tag="OO590_003275"
                     /pseudo
     CDS             1281..2565
                     /locus_tag="OO590_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
     gene            2617..3772
                     /locus_tag="OO590_003276"
                     /pseudo
     CDS             2617..3772
                     /locus_tag="OO590_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(3738..3860)
                     /locus_tag="OO590_003277"
     CDS             complement(3738..3860)
                     /locus_tag="OO590_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638540.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            4063..5748
                     /locus_tag="OO590_003278"
                     /pseudo
     CDS             4063..5748
                     /locus_tag="OO590_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            5786..8158
                     /locus_tag="OO590_003279"
     CDS             5786..8158
                     /locus_tag="OO590_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6638541.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNTPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            8215..10998
                     /locus_tag="OO590_003280"
     CDS             8215..10998
                     /locus_tag="OO590_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6638542.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETFESMG
                     LRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     SEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFLLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            11360..>11981
                     /locus_tag="OO590_003281"
     CDS             11360..>11981
                     /locus_tag="OO590_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6638543.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT         3471 a         2669 c         2610 g         3231 t
ORIGIN      
        1 actgctaata aaccagcacc atatttcggt aggttctgga cagatggttt agaactgtgc
       61 tcagtactgt tcgtcttaaa ataagtggcg agtgtcgaag aacattcttc gctaacgtca
      121 gatatgagat aacccgccgg gatttaatgc tgttagcctg atatttcagg tgtaaccctt
      181 tattttcagc gtgataaccg agtaaccaca ttacgatagt gctcaacgtt gccagcagac
      241 tcagaaccag aattcttcct gttgaacgac ttttgctggc ccgaagacca aatccgaagc
      301 gcccgttctt ttcatcacga aagttttgct ctatctgcat tcgccgactg tataacttga
      361 tgatttctcg cgctctgaag tcatttgtac tgctgaagat caaccaggct tcttttgctg
      421 atttgctctg ttctttgtct gttttgttta aaccactctg acctttcgaa cgtttctttt
      481 ttcgcccttt tgccgatttc ttataggtgt aaaaatgccc ccggatgctt tttttccttt
      541 ctttaaccag ccttccagcc cccatatatt ccggtgtttt acattcaggg ctatccgaca
      601 cttttaacca tctttcaggc gcgttatcga ggcaatactg aacgttattt ctgatccgac
      661 caataaaatc ccagcccaga gaagtaatat ggtgaaacca tgcgctctga aatccagcat
      721 ccgtgacaat aataaccctg gcatcggggg gcagggattg agcaagggaa tcaaggaaat
      781 catgttgtat caatggatta ttttgttttt cagaaggaac aaccttactc aataacggaa
      841 tggaacgccc gtcacacaga aggctggcac gaagaacatg atgctcctgg gagggatagc
      901 cactccagtc tacggctatt acacataaag ataactgccg tgtcagcatt gaagtgatat
      961 tcctgaaaat cattggaata tcgcgatgga gcgcctcatt acccatgagg cgatcaatac
     1021 gtttgatctt attcttaact tgtgcattac ccggcaaaaa acgtccgata ctggtctgcg
     1081 tcagagatgc tccgttgatt aacgccattg tggcatccat cagggcattt tgtcggtact
     1141 gatgaaatgg tgctaaggca tcccggaaga aattctgaca tactttatga acaggcatag
     1201 cagtgatctc attgaattgt tggcacaaat cagtagatca cataatgcta tgtttgtctc
     1261 gttttctggg gattcctcag ccctcccgtg ccgcaataat gaccggtcga gctcccgccc
     1321 gctttggtgt ctattccaat accgatgctc aggatggtat tccgctaaca gaaactttct
     1381 tgcctgaatt attccagaat catggttatt acactgcagc agtaggtaaa tggcacttgt
     1441 caaaaatcag taatgtgccg gtaccggaag ataaacaaac gcgtgactat catgacaact
     1501 tcaccacatt ttctgcggaa gaatggcaac ctcaaaaccg tggctttgat tactttatgg
     1561 gattccacgc tgcaggaacg gcatattaca actccccttc actgttcaaa aatcgtgaac
     1621 gtgtccccgc aaaaggttat atcagcgatc agttaaccga tgaggcaatt ggcgttgttg
     1681 atcgtgccaa aacacttgac cagcctttta tgctttacct ggcttataat gctccgcacc
     1741 taccaaatga taatcctgca ccggatcaat atcagaagca atttaatacc ggtagtcaaa
     1801 cagcagataa ctactacgct tccgtttatt ctgttgatca gggtgtaaaa cgcattctcg
     1861 aacaactgaa gaaaaacgga cagtatgaca atacaattat tctctttacc tccgataatg
     1921 gtgcggttat cgatggtcct ctgccgctga acggggcgca aaaaggctat aagagtcaga
     1981 cctatcctgg cggtactcac caccccaatg tttatgtggt ggaaaggaaa acttcaaccc
     2041 ggtaattatg acaagctgat ttccgcaatg gatttctacc caacagctct tgatgcagcc
     2101 gatatcagca ttccaaaaga ccttaagctg gatggtgttt ccttgctgcc ctggttgcaa
     2161 gataagaaac aaggcgagcc acataaaaat ctgacctgga taacctctta ttctcactgg
     2221 tttgatgagg aaaatattcc attctgggat aattaccaca aatttgttcg ccatcagtca
     2281 gacgattacc cgcataaccc caacactgag gacttaagcc aattctctta tacggtgaga
     2341 aataacgatt attcgcttgt ctatacagta gaaaacaatc agttaggtct ctacaaactg
     2401 acggatctac agcaaaaaga taaccttgcc gccgccaatc cgcaggtcgt taaagagatg
     2461 caaggcgtgg taagagagtt tatcgacagc agccagccac cgcttagcga ggtaaatcag
     2521 gagaagttta acaatatcaa gaaagcacta agcgaagcga aataactaaa ccttcatgcg
     2581 gcggattttt ccgccgcctt attgagcgag atagcaatgc acgttacagc caagccctcc
     2641 agttttcaat gtaatctcaa atgtgattac tgtttttacc ttgaaaaaga gtcgcagttt
     2701 actcatgaaa aatggatgga tgacagcact ctgaaagagt tcatcaaaca atatatcgaa
     2761 gcgtctggca atcaggtcta ttttacctgg caaggcggtg aacccactct ggctggcctg
     2821 gattttttcc gtaaagttat tcactatcaa caacgctatg caggccaaaa acgtattttt
     2881 aatgcattac aaacgaatgg cattttattg aataatgaat ggtgtgcctt tctcaaagaa
     2941 catgattttc tggttggtat ctcgatcgat ggcccccagg agttacatga ccgttacaga
     3001 cgcagtaatt caggtaacgg tacttttgca aaagtgatag cagccatcga gcgtctgaaa
     3061 tcatatcaag tagagtttaa tacgttaacc gtcattaata acgttaatgt ccattaccct
     3121 cttgaggttt atcatttttt aacatctatc ggcagtaaac atatgcaatt tatcgaattg
     3181 ctagaaaccg ggacgccgaa tattgatttc agtggtcata gtgagaacac attccgtatc
     3241 attgattttt ctgtgcctcc cacggcttat ggcaagttta tgtcaaccat ttttatgcaa
     3301 tgggttaaaa acgatgtggg tgaaattttc attcgtcagt ttgaaagctt tgtcagccgt
     3361 tttttgggga atgggcatac aagttgtatt ttccaggagt cctgcaagga taatctggtt
     3421 gttgaaagta atggagacat ttacgaatgc gaccattttg tctatccaca gtacaaaatt
     3481 ggaaacatta ataaatctga actcaaaacg atgaacagtg tacaactgac agcgcaaaaa
     3541 acggatttca gcgaaatgtc agcaatgtgc atataaacct atctgcaatg gcggttgtcc
     3601 taagcatcgt attactaaag taaacaatga gactgtttct tatttttgcg aaggttataa
     3661 aatccttttt tcaaccatgg taccttatat gaacgccatg gttgagttag ctaagaacag
     3721 agtaccgctt taccacatta tggatgttgc aaaacaaatg gagaataatt aataacgctc
     3781 tcttttaact ccattcttag cctccttctt atgaccttaa caaggcaccc gacataaatt
     3841 cctgtcgggt gtttcgtcat catcatcttc ccttgcgaaa atcctggctt acgtggtatg
     3901 cgtcagggca ttagcaatac ccttgattat caatagcata accataacca ctgaaaatta
     3961 aaaatttcta tatatttcat attgttaaat gatacttaaa taaatttcat cagtaaatca
     4021 ttgccataga taatcattgg atggcacaat caattttcgc taatgataac cattcccatt
     4081 acgttttgta tgttgatagc aaagtatctc tgtctgctga agcccttttg gctgcgtaag
     4141 aataataaaa cttcagtgtt gttaatcatt attatcctcg caatgatcct tggcgtggtt
     4201 aaaattcagg tctggttaaa cgactggaat aatgattttt tcaatgcgct aagccagaaa
     4261 gaaaccgata agctttggca gcttgttctt tggttccctg ccctgctggg gatctttgta
     4321 ttaatatccg taaacaaaac atggttaatt aaactactga ctattcgctg gcgcgaatgg
     4381 ttaacagatt actaccttaa tcgctggttt gcagataaaa actactactt cacgcagatc
     4441 tacggcgagc ataaaaatac agataaccct gaccaacgta tcgctgaaga cattctctta
     4501 ctgatcaaca aaacgctcag tctttcattt ggcttcatcc agtcacttag catgctgatc
     4561 acctttaccg ttattctttg gcaaagcgcg ggtacgctct cattcactgt tggtggaaca
     4621 gaatggaata tccaggggta tatggtctat accgtcgtgc tcattgtgat cggtggaact
     4681 ttatttaccc ataaggtggg taaacgtatt cgtccgctta atgtggaaaa acaacgtagc
     4741 gaagcgacat ttcgaactaa tcttgtgcag cataataagc aggcagaact gattgcgcta
     4801 agcaatgctg agtctctaca acgccaggag ttgcgcgaca attttcatac cattaaagag
     4861 aactggcatc gtttaatgaa tcgtcagcgg tggcttgatt actggcaaaa tatttattcg
     4921 cgctcgctta gcgttcttcc ctactttctg ttattgccgc agtttattag cgggcagatc
     4981 aatctgggcg gactgatgaa atcgcgccag gcatttatgc tagtatcgaa caatttaagc
     5041 tggtttattt ataaatatga cgaacttgct gaactggctg cggttatcga tcgcttgtat
     5101 gagttccatc aactcactga atagcgccct acgaataagc ctaaaaattg ccaacatgcg
     5161 gtacaagtgg ctaatgcgag tattcgtacg cctgataata agatcatatt agagaacttg
     5221 aactttcatg tttcgccagg caaatggcta ttactgaaag gctactctgg tgcgggaaaa
     5281 accacactgc ttaaaacatt atcccactgc tggccgtgat ttaaaggtga tatttcttct
     5341 cctgctgaca gttggtatgt gtcacaaaca ccgttaatca aaagcggctt actgaaagag
     5401 attatttgta aagcacttcc cctgcccgta gacgataaat cgttgagcga agtactgcat
     5461 caggttggtc tggggaaatt ggctgcgcgt attcatgacc acgatcgctg gggagatatt
     5521 ctttccagcg gcgaaaaaca acgtatcgcc ctggcacgat taattttacg acgtccgaaa
     5581 tggatatttc ttgacgaaac tacctctcat cttgaggaac aagaggctat acgcttactg
     5641 cgtttagtgc gtgaaaaact acccacaagc ggcgtcatta tggttacaca tcaacccggt
     5701 gtctggaacc tggctgatga tatttgtgac attagcacgg ttttataaca acaataaaag
     5761 ccctgctgta cggagatata aataaatgaa gcgagttctt attcctggcg tcattttatg
     5821 tggcgctgat gtggcgcagg ccgtcgatga caaaaacatg tacatgcatt tttttgaaga
     5881 gatgacggtc tatgctcctg tccctgtacc cgtaaacggc aacacgcatt acaccagtga
     5941 aagcatcgag cgtttaccga ccgggaatgg caatatcagc gatctgctga gaaccaaccc
     6001 tgcggtacgc atggattcaa cgcaaagtac ctcgttgaac cagggagata ttcgccctga
     6061 gaaaatctct attcacggtg cgtcgcccta ccagaatgcc tatttgattg acggtattag
     6121 tgcaactaat aacctgaacc cagcgaatga gtccgatgcc agtagtgcaa ccaatattag
     6181 cgggatgtca caggggtatt atcttgatgt cagcttactg gacaatgtga cgctttatga
     6241 cagttttgtg ccggttgaat ttggtcgctt caatggcggg gtaattgatg caaagatcaa
     6301 acgcttcaac gctgatgata gcaaggtgaa attgggttat cgcactacgc gtttggactg
     6361 gttaacatcg catatcgatg agaataacaa gagcgcattt aatcaaggtt cttcaggaag
     6421 tacctatttc tctccagatt ttaaaaagaa cttttatacc ttgtcgttta atcaggagct
     6481 ggctgataac tttggcgtta ccgccggttt atcgcgccgc cagtctgata tcacccgcgc
     6541 ggattatgtt tcgaatgacg gcattgtcgc cggtcgggca cagtataaaa acgttatcga
     6601 tactgcattg agcaaattta cctggtttgc cagcgaccgc tttacccacg atttaacctt
     6661 aaaatatacc ggctccagcc gtgattataa taccagcacc ttcccgcaat ctgatcgcga
     6721 aatgggtaat aaatcctatg gtctggcatg ggatatggat acgcaactcg catgggccaa
     6781 actacgtacc accgttggtt gggatcatat tagtgattat acccgtcacg atcatgacat
     6841 ctggtacacc gaactttcat gtacatatgg tgatattaca gggcgttgta cccgtggcgg
     6901 attaggacac atttcccagg ctgtagataa ttacaccttc aaaacacgcc tggactggca
     6961 aaaattcgcc gtgggtgatg tttcgcatca accctacttc ggcgcggaat acatctattc
     7021 cgatgcatgg actgaacgcc ataaccagtc tgaatcctat gtgattaatg ctgcaggaaa
     7081 gaaaactaac cataccattt accataaagg taaaggcagc ctgggaattg acaactacac
     7141 gctgtatatg gcggatcaca ttagctggcg gaatgtgtcg ttaatgcccg gtgtgcgtta
     7201 tgactatgac aactatctgt caaaccacaa tatctccccg cgctttatga cggaatggga
     7261 tatttttgct gatcaaacct caatgattac cgccggttat aaccgttact atggcgggaa
     7321 tattcttgat atgggattac gtgatatccg caatagctgg acggaatcgg tatcaggtaa
     7381 taaaaccctg acgcgttatc agaatttgaa aacgccttat aacgatgaac tggcaatggg
     7441 attgcagcaa aaaatcgata agaacgttat tgcacgcgca aactatgttt accgtgaagc
     7501 gcatgatcaa atcagcaaaa gcagtcgtac cgacagcgcg actaaaacca ccattactga
     7561 atataacaac gatggcaaaa ccaaaacgca ttcgtttaac ctcagttttg aactggccga
     7621 acccctgcat atccgccagg tagatattaa cccgcaaatt gtctttagct atatcaagag
     7681 caagggcaac ttgtcgttaa acaatggtta tgaggagagc aataccggtg ataaccaggt
     7741 ggtttataac ggtaatctgg tctcttacga tagcgttcca gtggcagatt ttaatacccc
     7801 attaaagatc tccttaaaca tggatttcac gcatcaaccg agcgggttag tgtgggcgaa
     7861 tacgctggcc tggcaagaag cgcgtaaagc tcgcattatc cttggtaaga caaatgcgca
     7921 atacatcagc gaatattcag attacaagca gtatgttgac gaaaaactgg atagcagcct
     7981 gacctgggac acccgcttgt cctggacgcc acaatttctg aaacaacaaa acctgacgat
     8041 cagtgccgat attctcaatg tactggatag caaaaccgct gttgatacaa cgaacaccgg
     8101 tgtggcgacc tacgccagtg gccgtacttt ctggcttgat gtcagcatga aattttaata
     8161 gttcggattt tacttaaccg ggtaaattcg cccggttttc gcatggagat aattatgaga
     8221 aacctctgtt tcttactgac gttagtggca actctgttgc tccccgggcg gctgattgcc
     8281 gccgccttac agcaggatga aaagttaatt accgggcaac tggacaatgg cttgcgatat
     8341 atgatttatc cgcatgctca tccaaaggat caggtaaatt tatggctgca aattcatacc
     8401 ggttcattgc aggaagaaga caatgagcgc ggcgtggctc attttgtaga acatatgatg
     8461 tttaacggcg caaaaacatg gccgggtaat aaagtcatcg aaacatttga gtcaatgggc
     8521 ctgcgttttg gtctcgatgt taatgcctat accagctatg acgaaacggt gtatcaggtg
     8581 agtttgccga ctacgcagaa acaaaatctg caacaagtga tggcaatctt cagtgaatgg
     8641 agtaatgccg caacctttga aaaactcgaa gtagacgctg aacgtggcgt aatttctgag
     8701 gaatggcgtg cccatcagga tgcgaaatgg cgcacctctc aggcgcgccg ccctttcctg
     8761 ctggctaata cccgtaattt agaccgtgaa cctatcggcc tgatggatac tgtcgccacg
     8821 gtcacaccgg cacaattgcg ccaattttat caacgctggt atcaaccaaa taatatgacc
     8881 tttatcgtgg tcggcgatat cgacagtaaa gaagcgctgg cgctgataaa ggataattta
     8941 agtaagtttc cggctaacaa agcagctgaa aatcgcgtct ggccgacaaa agccgaaaac
     9001 cacctgcgct ttaatatcat taatgataaa gaaaaccggg tgaacggcat cgcactctat
     9061 tatcgcctgc caatggtaca agtgaacgat gagcaaagct ttgtcgaaca agctgaatgg
     9121 agcatgttag ttcagctgtt caatcaacgt ctgcaggaac gcatacagtc gggcgagttg
     9181 aagactattt ctggcggcac tgcgcgcagc gttaaaattg cacccgatta tcagtcgctg
     9241 tttttccgtg taaatgcacg agacgataat atgcaggatg ctgcgaatgc attaatggca
     9301 gagttggcaa ccattgatca gcatggtttt tctgctgaag aactcgatga tgtcaaatct
     9361 acccgcctca cctggctgaa aaatgcggtt gatcagcaag ctgaacgtga tttacgtatg
     9421 ctgaccagtc gcctggcatc cagctcatta aataatacgc cgttcttgtc gccggaagag
     9481 acatatcaac tttcgaaacg tctgtggcag caaattaccg tgcaaagtct ggcggaaaaa
     9541 tggcagcagt taagaaagaa ccaggacgca ttttgggagc aaatggtaaa caatgagctt
     9601 gccgccaaaa aagcattgtc tcctgcggct atcctggcgc tggaaaaaga gtacgccaac
     9661 aaaaagctgg cggcttacat cttcccaggc agaaatttat cgttaacagt agacgctgac
     9721 ccacaggcgg aaattagcag caaagaaacg ctggctgaga atctgacatc attaacactt
     9781 tccaatggtg ccaaggttat tctggcaaaa tccgcgggtg aagagcaaaa gctacaaatt
     9841 attgccgtat cgaataaagg cgatttaagt ttccctgcgc agcaaaaatc acttatcgcg
     9901 ctggcaaata aagcagttag cggaagcggc gttggcgaac tctcctcttc cagcctgaaa
     9961 cgctggagtg cggaaaactc ggtaaccatg agcagtaaag tcagtggcat gaatacgttg
    10021 ctctccgtta gcgcgcggac taataaccct gaacctggtt tcctgttgat taaccagcga
    10081 atcacccaca gcacgattaa cgacaatatt tgggcatcgc tacaaaatgc tcaaattcag
    10141 gcgttgaaaa cgctcgacca gcgtccagcg gagaaattcg cccagctgat gtatgagacg
    10201 cgctatgctg atgaccgcac gaaattactg caagaaaatc agattgcaca gtttactgcc
    10261 gcagatgcgc tggctgccga tcgccagttg ttttcatctc cagcggatat cacgtttgtc
    10321 attgtcggta atgtcgcaga agacaaactc gtggcgttaa ttacgcgtta cttaggctca
    10381 atcaaacact ctgattcgcc attagccgca ggtaaaccat taactcgcgc gacggacaac
    10441 gcatcggtta ctgtaaaaga acaaaatgaa cctgtggcac aggtttcaca gtggaagcgt
    10501 tatgattccc ggacacctgt taatctggcg acgcgtatgg cgctcgatgc ttttaacgtc
    10561 gcactggcaa aagatctacg cgttaatatt cgtgaacagg catctggggc atacagcgtt
    10621 tcttctcgcc tctcggttga tcctcaggct aaagatatca gtcatttgct ggcttttact
    10681 tgtcaaccag aacgacatga tgaacagtta acgttagcga atgaagtgat ggttaagcgt
    10741 ttgactaagg ggatcagtga gcaagaactg aacgaatacc agcaaaacgt tcagcgcagc
    10801 ctcgatatcc aacagcgtag cgttcaacaa ttagcgaaca ctattataaa tagtcttatt
    10861 caatatgacg atcctgcagc atggactgag caggagcaat tgttgaaaca aatgacgata
    10921 gagaatgtta acactgccgt taaacaatat ctttctcatc cggtaaatac ttataccgga
    10981 gtattattgc caaaataata acagccccgt caacacatcg ttggcgggga ttttagcaat
    11041 attcgctatt tttatgtaat aattttataa atgcgttcaa aataataacc aagtactaat
    11101 agtgatattt taatgtctga tttttacgtg ataattcagg aggcacagaa agcgcataaa
    11161 aataacaaca taaaacacct tgccaccacc caaaaattct ctattgcatt gtttttaatg
    11221 aaaaaaatat cattccaaca atatctcacg ataaataaca ttaggatttt gttatttaaa
    11281 cacgagtcct ttgcacttgc ttactttatc gataaatcct acttttttaa tgcgatccaa
    11341 tcattttaag gagtttaaaa tggataagaa gcaagtaacg gatttaaggt cggaactact
    11401 cgattcacgt tttggtgcga agtctatttc cactatcgca gaatcaaaac gttttccgct
    11461 gcacgaaatg cgcgacgatg tcgcatttca gattatcaat gatgaattat atcttgatgg
    11521 caacgctcgt cagaacctgg ccactttctg ccagacctgg gacgacgaaa acgtccacaa
    11581 gttgatggat ttatccatta acaaaaactg gatcgacaaa gaagaatatc cgcaatccgc
    11641 agccatcgac ctgcgttgcg taaacatggt tgccgatctg tggcatgcgc ctgcgccgaa
    11701 aaatggtcag gccgttggca ccaacaccat tggttcttcc gaggcctgta tgctcggcgg
    11761 gatggcgatg aaatggcgtt ggcgcaagcg tatggaagct gcaggcaaac caacggataa
    11821 accaaacctg gtgtgcggtc cggtgcaaat ctgctggcat aaattcgccc gctactggga
    11881 tgtggagctg cgtgagatcc ctatgcgccc cggtcagttg tttatggacc cgaaacgcat
    11941 gattgaagcc tgcgacgaaa ataccatcgg cgtggtgccg a
//
LOCUS       DAJTYF010000114        11943 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTYF010000114 DAJTYF010000000
VERSION     DAJTYF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.114"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..403)
                     /gene="yehX"
                     /locus_tag="OO590_003282"
     CDS             complement(<1..403)
                     /gene="yehX"
                     /locus_tag="OO590_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6638544.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQ"
     gene            complement(396..1554)
                     /gene="yehY"
                     /locus_tag="OO590_003283"
                     /pseudo
     CDS             complement(396..1554)
                     /gene="yehY"
                     /locus_tag="OO590_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(1561..2478)
                     /gene="osmF"
                     /locus_tag="OO590_003284"
                     /pseudo
     CDS             complement(1561..2478)
                     /gene="osmF"
                     /locus_tag="OO590_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
     gene            complement(2730..5027)
                     /gene="bglX"
                     /locus_tag="OO590_003285"
     CDS             complement(2730..5027)
                     /gene="bglX"
                     /locus_tag="OO590_003285"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6638545.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            5223..6938
                     /gene="dld"
                     /locus_tag="OO590_003286"
     CDS             5223..6938
                     /gene="dld"
                     /locus_tag="OO590_003286"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6638546.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(6976..7908)
                     /gene="pbpG"
                     /locus_tag="OO590_003287"
     CDS             complement(6976..7908)
                     /gene="pbpG"
                     /locus_tag="OO590_003287"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6638547.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(8082..8669)
                     /locus_tag="OO590_003288"
     CDS             complement(8082..8669)
                     /locus_tag="OO590_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6638548.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            8839..9417
                     /locus_tag="OO590_003289"
     CDS             8839..9417
                     /locus_tag="OO590_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6638549.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(9534..10295)
                     /locus_tag="OO590_003290"
                     /pseudo
     CDS             complement(9534..10295)
                     /locus_tag="OO590_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(10348..11784)
                     /gene="mdtQ"
                     /locus_tag="OO590_003291"
     CDS             complement(10348..11784)
                     /gene="mdtQ"
                     /locus_tag="OO590_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR6638550.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGMETDINASKTRQQLNDVVGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
BASE COUNT         2825 a         3300 c         3027 g         2791 t
ORIGIN      
        1 gctgatgcgg ataacgctca cgcaaatttg actccagccc cagtagcgcc attaattcgt
       61 cgatacgatc gtcaatccgc gcccgcgacc atttttgtaa ttgcggcacg gtagcgatgt
      121 tttgcgccac gctccagtgg gggaacaggc caatagattg aatggcatag cccatccggc
      181 ggcgcaactc cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg atcactccgc
      241 tgtcatgctc caccaggcgg ttaatcattt tcagggtggt ggatttgccg gagccagatg
      301 tgccaatcag caccgaaaaa ctcccttcct gaaaattgag attgagatcg ttaacggctt
      361 tttgtgcgcc gaacagtttg ctgacatggc taaattcaat cattacgttt caccttcagc
      421 agtgcgataa gcaaatcgaa cagcgcgtcg gtaagcaccg ccagaacaat taccgggatc
      481 acccccagca acactaaatc aatggcgctg cttagcagcc cctggaaaac cagcgcacca
      541 aaaccgcctg cgccgattaa cgccgcaatc accgccatac ctacagtttg caccatcacc
      601 acccgcaggc tgcgcagaaa taccggtaac gccagcggta actgaacatg caggaaacgc
      661 tgcgccccgc tcatccccat cgctctggcg ctctccagca catcgcgcgg gatctggttc
      721 aaaccgacta ccacgccgcg caccagcggc agcaaggcat agagcaccag cgcaatcagt
      781 gcgggtgtca ttccggttcc tgctatgccg agcttcccca gccacggaaa ggccgtaacc
      841 agcgcggcaa gcggcgcaat caacaggcca aagagcgcca ccgaaggcac ggtctgaatg
      901 acattgagca gagaaaaaat tgccccctgc cgcgcagtgg aaaagtagca ccagatgccc
      961 aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt cagatgttgt
     1021 gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattta gtgagagatc
     1081 gttaagcgtg ccggagtaca gcaaccacag cggaataatg gcaatctgca tatgcaacaa
     1141 ccagcgccac agcggatgcg tggagattcg gcggatggca tcgctacagg ccagcaatgc
     1201 cagcgccgcc gccagccaga aaccactgcc gaggctggta cgcgccagcg cactgccatt
     1261 ttgcgccagt tgggtcgccg cctttccagc tccccacacc aacaatacga agacgaattg
     1321 cgccagaatg agtgcaaaaa tgctgccttt tttagcgggg acaaaacagg ctgtcagcca
     1381 ggcgcaacca acgcccacca gcatccagat cgtttgcggc cacagttgcc agagatgaag
     1441 cccttcaccc gagactaaac gattaggcgc gtaactgata aacggcagcg ccgctgcgat
     1501 tgccgtcagc aacaacagca gcgccagaac aggattaata cgtagataag tcacgggaaa
     1561 ttacttcgcc cacccttttt gtttcaggta gtcggcagcc acttttttgg catccagtcc
     1621 ttccactgca atgctggcat tcagttgctg caatgttttt gcatcgaggc tggcgaagac
     1681 tggctgtagc cactgtgcca tttgcggata ctccttcaac accgattcac gcaccactgg
     1741 tgcaggcgcg tagataggtt gcacgccttg cggatcgctt aaggtttgca gccccagcgc
     1801 cgcgaccgga gcgtcagtgc cgtaagccat tgcggcatta acgccagagg tttgctgggc
     1861 agcggcttta atcgtcaccg ccgtgtcgcc accagccagt gacagcaact gatcctgacc
     1921 gagcttaaag ccataggctt tttcaaacgc gggtaacgca tcggcgcgtt cgataaactc
     1981 tgccgaggct gccagtttga aggtgccgcc ctcttgcaga taacgactca ggtcggcaag
     2041 cgaagtgagt ttgtttttct ctgccacatc ctgacgcacg gcgatagtcc aggtgttatt
     2101 tgcaggcgcg ggcgtcagcc agattaactt gttgtgctcc gcatcgagct ttttgacttt
     2161 ctcgtagccc tgcccggcat ttttccacgc tgcatcgttt tcatctttaa agaagaaagc
     2221 gccattgccg gtatattccg gatagatatc cagttcaccg gaagtaatcg ccccgcgcac
     2281 cacaggagtc gttccgagtt gcactttatt caccgtcggt acgccgtggc tttcgagtac
     2341 ctgcaagatg atgttgccga gcagcgcgcc ttcggtatcg atttttgaac cgactttaac
     2401 gggggaagcc gcctatagca gcaggctcac ggctgccaac ataaccagtg aacctgccca
     2461 gagctttaag agtggcatga tgctttcctc attcttttac tgttgttttc agcgaattaa
     2521 gagaaaagca tagttgataa tgacagagtt agcctgagcg gaaggattca gttgcagaat
     2581 cagataaata cttaagaggc atattcggtc cggcgtttcc tgccggatgc ggcgcgagcg
     2641 ccttatccgg cctacaaagg gcgaaaattc agcaagttaa gatatgaaca tcaggcaaag
     2701 ggcgcaaacg tcgcgccctt actaaagcat tacagcaact caaactcgcc tttcttaacg
     2761 cgtgcggaat cagtgccgat aaatacattg aacttgccag gctcggcgtc atatttcatc
     2821 tgttgattcc agaacttcag cgcctcaata tcgatcggga agctgacagt ctgagtttcg
     2881 cccggtttca gggtgatttt ctcaaagcct ttcagctgtt tcacagggcg actcatggaa
     2941 gccgtcacat cctgcaagta catctgcact accgtggccc cctcgcgctt accggtgttc
     3001 gtcacctgca cgctggccgt cactttgccg tcacgcttca tggtcggcgc agaaagtttc
     3061 acatcagaga cggtgaaagt ggtatagctc agaccatagc cgaacggata aagcgccccg
     3121 ttagcttcat caaaataacg cgaagtgtat ttgttcggct tgtcggcatt atacggacga
     3181 ccggtgttca gatggctgta gtacaccggg atctgcccga cagaacgcgg gaaggacatt
     3241 ggcagcttgc cggacgggtt gtaatcgcca aacaatacat cggcaattgc attaccgcct
     3301 tcagtccccg caaaccaggt ttccagaatc gcatcagcct gctgatcttc tttcaccagc
     3361 gccagcggac gcccgttcat cagcaccagc accagcggtt taccggtggc tttcagcgcc
     3421 gcaatcaagt cacgttggcc ttgcggaata gtgatatcgg tccggctgga ggcttcgtgc
     3481 gccatcccct gtgcttcacc gactacagcc accaccacat cagattgttt cgccgtctgc
     3541 accgcttcat caatcatctc ttgcggcgaa cgcggatcga ctttgaccgc ttcttcatac
     3601 tgattcagga aatcgataat gcctttgtca ctggtaacgt tcgccccttt ggcatacagc
     3661 actttaccgt tttcaccgac ggaattttta atcccggtca gtacggtcac ggattgatcg
     3721 gcaacaccgg ctgcggacca gctgcccatc acgtcacgtt tactgtccgc cagtggccca
     3781 accaccgcaa tggtggccga ttttttcagc ggtaacgttt cgagacggtt tttcagcaac
     3841 actaggcttt cgcgcgccac ttcacgcgct tctttacggt gcaggcggct ttcggcattg
     3901 gtatccaccg ggtcagactc tttcggcccc aaatggctgt atgggtcgtt aaacaacccc
     3961 atatcatatt taacgttcag tacatggcgg gcagcatcgt ccagctctgc catggtcact
     4021 ttgccggatt taatcaaccc aggcagatac ttcgagtagt actcgtcgct catactcatg
     4081 ttgattccgg atttcagcgc cacgcgcacc gcatcttccg ggtctgccgc cgtgccatgt
     4141 ttaatcagct ctttgattgc accgtgatcg gaaacggtga tgcctttaaa gccccactgg
     4201 tcgcgcagaa catctttcag cagccaggaa tcggaggtgg ctggcgtgcc gttcagcgag
     4261 ttcagcgcca ccatcaccgc gccgctgcct gcgtccagcc ccgctttgta cggcggcata
     4321 taatcattaa acaggcgctg cggactcata tcgacggtgt tgtactcttt accgccttct
     4381 accgcgccgt atgcggcaaa gtgtttgacg ctggtcatca ccgagtaacg atctgccggg
     4441 cttttaccct gcatcgcttc caccatggtt ttacccatta ttgaggtgag atacgtatct
     4501 tcgccaaaac cttcggaagc acgtccccag cgcggatcgc gcgagacatc gaccatcggt
     4561 gcccaggtca tattcaggcc atcatctgcc gcttcataag cagagacacg cccgaccgtt
     4621 ttcaccgcat cgaggttaaa agacgaggcc agaccgaggc taatcgggaa caccgtgcgc
     4681 tgaccgtgca gcacgtcgta agcaaagaaa agaggaattt tcaggcggct taattccatc
     4741 acctgatcct gcatggcgcg gatatcctga cgggttacgg tgttgaaaat cgccccaacc
     4801 tgaccgtctt tgatcatctc gcggatcgcc tctttcggat tatccgggcc tacgctgatt
     4861 aagcgcagct gaccaatttt ctcatcaacc atcattttct tgagcagatc ggtgacgaac
     4921 gcatcccgcg cttcaggcgt taatggatgg ttaccgaata aatcatccgc cagtgctggc
     4981 tgcaggacca gactcaccgc gattcctact gaacatagcc atttcatatg gatttttctt
     5041 cctttgttgc ccgacgtggc agcgaaaatg gtgcaaaaac cgtagtttgc cataagcatg
     5101 atggagagag aaaaagaata ctcagtttat tgtctgaatt ttcaaaatat tcactcgctg
     5161 aattgtcata caaggcgcta ttctagtttg tgatattttt tcgccaccac aaggagtgga
     5221 aaatgtcttc catgacaaca actgataata aagccttttt gaatgaactt gcccgtctgg
     5281 tcggtcattc acacctgctc accgatcccg caaaaacggc ccgctatcgc aagggcttcc
     5341 gttctggtca gggcgacgcg ctggctgtcg ttttccctgg ctcactactg gaattgtggc
     5401 gggtgctgaa agcctgcgtc accgccgaca aaattattct gatgcaggct gccaatacag
     5461 gcctgaccga aggatcgacg ccaaacggta acgactatga tcgcgatatc gtgatcatca
     5521 gcaccctgcg tctcgacaag ctgcacgttc tcggcaaggg cgaacaggtg ctggcctatc
     5581 cgggcaccac gctctattcg ctggaaaaag ccctcaaacc gctgggacgc gaaccgcatt
     5641 cggtgattgg atcatcgtgt ataggcgcat cggtcatcgg cggtatttgt aacaactccg
     5701 gcggctcgct ggtgcaacgt ggcccggcgt ataccgaaat gtcgttattc gcgcgtatag
     5761 atgaagatgg caaactgacg ctggtgaacc atctggggat tgatctgggc gaaacgccgg
     5821 agcagatcct tagcaagctg gatgacgatc gcatcaaaga tgacgatgtg cgtcacgatg
     5881 gtcgtcacgc ccacgattat gactatgtcc accgcgttcg tgatattgaa gccgacacgc
     5941 ccgcacgtta taacgccgat ccggatcggt tatttgaatc ttctggttgc gccgggaagc
     6001 tggccgtctt tgcggtacgt cttgatacct tcgaagcgga aaaaaatcag caggtgtttt
     6061 atatcggcac caaccagccg gaagtgctga ccgaaatccg ccgtcatatt ctggctaact
     6121 tcgaaaatct gccggttgcc ggggaatata tgcaccggga tatctacgat attgcggaaa
     6181 aatacggcaa agacaccttc ctgatgattg ataagttagg caccgacaag atgccgttct
     6241 tctttaatct caagggacgc accgatgcga tgctggagaa agtgaaattc ttccgtccgc
     6301 attttactga ccgtgcaatg caaaaattcg gtcacctgtt ccccagccat ttaccgccgc
     6361 gcatgaaaaa ctggcgcgat aaatacgagc atcatctgct gttaaaaatg gcaggcgatg
     6421 gcgtcggtga agccaaatcg tggctggtgg attatttcaa acaggccgag ggcgatttct
     6481 ttgtctgtac gccggaggaa ggtagcaaag cgtttttaca ccgtttcgcc gctgcgggcg
     6541 cagcaattcg ttatcaggcg gtgcattccg atgaagtcga agacattctg gcactggata
     6601 tcgctctgcg gcgtaacgac accgagtggt atgagcattt accgccggag atcgacagcc
     6661 agctggtgca caagctctat tacggccatt ttatgtgcta tgtcttccat caggattaca
     6721 tagtgaaaaa aggcgtggat gtgcatgcgt taaaagaaca gatgctggaa ctgctacagc
     6781 agcgcggcgc gcagtaccct gccgagcata acgtcggtca tttgtataaa gcaccggaga
     6841 cgttgcagaa gttctatcgc gagaacgatc cgaccaatag catgaatccg gggatcggta
     6901 aaaccagtaa acggaaaaac tggcaggaag tggagtaaaa attacggatg gcagagtatc
     6961 gccatccgaa ttcacttaat cgttctgtgc cgtctgcccc gccgccgcca tttgggcggc
     7021 tttttgtttt ttatagctca acgctgctgc cggcacaggc atcactttac cggtttcaat
     7081 ccaggtacgc aggcggctgg catcggcaaa atgggtatat ttgccaaacg cgtccatcac
     7141 taccagcgcc accggtttat tattgataac cgtacgcatc accagacaat ggcccgccgc
     7201 attggtaaag ccggttttgg ttaactgaat attccagtta tcgcgataca ccagatgatt
     7261 agtattgcgg aacggcagcg tatacgtcgg gttagagaag gttgccatat cttcccgggt
     7321 agtacttaac tgcccgatca acggatattg tttgctggca atgagcagtt tggttaagtc
     7381 acgggcagtt gaaacgttat gcaccgacaa tccggtaggt tcaacaaagc gcgtgttgtt
     7441 cattccgagc gatttcgctt tcgcattcat tgccttaata aaggctttgt aaccaccggg
     7501 ataatggtgc gcaaggcttg ccgccgcgcg gttttctgaa gacatcagcg ccagcaacag
     7561 catatcttta cggctgattt cgctattcag tcgtacgcgc gaatagaccc ctttcatctc
     7621 cggcgtctgg ctgatatcca cttttagttt ttcatccagc ggcagtcgtg catccagcac
     7681 aaccatcgcg gtcattaatt tgctgataga cgcaatcgga cgcaccagat ccgggtggtt
     7741 cgaatagatc actttgttgg tattcagatc aacaatcatc gcgctaccgg aggcaatttc
     7801 cggttgtgaa gcggtggtag cggctgccgt tttcgcaacg gcctgcggtg caaaaggcac
     7861 agccagcatc agggccaggc taaataaaga aactcgaaat ttcggcatga tgagcattca
     7921 gatagtggtt cacgcgcacg ggttgcgcac cgccggagta aggatttact gaggctagcg
     7981 acgccatcat aacgagcaaa aagtgcgatc gtcaaaggag aatcgtgagt aaatgctgca
     8041 ttgctgacat ttacgccagc aatgcaacgt caaagaactt tctagaacaa acgataaccg
     8101 tagccccata gtataacggt tagggtgagc agcacttcca gtaccagcac gccaatcgcc
     8161 agcgtcgaac tggagaagct aaggccttcc tctttgttga tattcaggaa gctcggaata
     8221 ccaaggtaaa gcaggtagcc ggtgtaaaac agcgccaccg tgccgaccag cgcacacaac
     8281 cagaccagtg gataaagcgc caccagaccg cttaaaaaca gcggagttgc aacgtagccc
     8341 gcgaagacca tgcagtgcgc aagagacgga cgctgcgggt aattgcgcgc catccaccag
     8401 atgacccgcc ccatcaccgc aaccccagcc agcataacgc cgtaaaacaa gacagccagc
     8461 gccagtccgg taaaccagga taacttcagg atagtgccat cgccaaaatt ccagccaatc
     8521 tgtgtagtgc caatgaaggc gcaaatcacc ggaatcgccg ccatcagcaa aacgtggtgg
     8581 gtgtaatggt gagaaatcgt ttcgttttcg cgattaatca cctgcatttc acgatcggga
     8641 tgggaaaaca gtccccagac atggctcata ccgccccctt gttgtgagtt catgaacctg
     8701 acagttcaag tataagccag cttgtgatta ttttttgttc atccatgtaa tttgcaccgt
     8761 ctgatgtatg tcctttttca gcgtgaatga ttcacagggt gtatgcttac aggcaaccaa
     8821 agggagacag actggcctat ggatctcaat acactcatct cacaatatgg ttatgccgcg
     8881 ctggttatcg gtagcctagc ggaaggtgaa accgtgactt tgctgggagg cgttgcggca
     8941 catcaggggc tattaaagtt cccactggtg gtactttctg tggcgcttgg tggcatgatt
     9001 ggcgaccagg tactctatct gtgcgggcgg cggtttggcg gcaagctgtt acgccgtttc
     9061 tcgaaacatc aggataaaat tgagcgggcg cagaaactta tccaacgcca tccgtatctg
     9121 tttgtcattg gtacgcgctt tatgtatggc tttcgggtga ttggcccgac gctgattggt
     9181 gccagccagc tgccgccgaa aatctttctg ccgctgaata ttctcggcgc atttgcctgg
     9241 gcgttgattt ttaccactat tggttacgct ggtggtcagg tgattgcgcc gtggttgcac
     9301 aatctcgacc agcatttgaa gcactgggtc tggttgattc tggtcgtggt tctggtggtg
     9361 ggcgtgcgct ggtggctgaa acggcgcggg aagaaaaagc cggataatca ggcgtaaagc
     9421 agttgccaca gataagcatt cactccacat ccggcaccgg gtgttccatg cctgatgcga
     9481 cgctggtgtc ttatctggcc taacccattg aaatatccac gcacctcact gcgctattct
     9541 gggttgaact gcggattcgc caacataaag ccgccatcca ctatcaacga ctgccctgtc
     9601 gtgtaatttg cgccctccga acaaagccac accaccaggc tggcaatctc atgcgttgcg
     9661 ccaaaacgcc gtagaggaat cgaaggctcc gcgtcgggct tcacgtcgct atcatccatg
     9721 ctgttcattg gcgtggctat cgccccaggc gcgactgcgt tcaccaaaat cttatgcctg
     9781 accaactcca gcgccatcgt tttggttaac ccgccgagcg catgtttagc ggctgtgtag
     9841 gcgctggcat ccggcagcgg cgtatgttca tgtaccgacg taatgttgat gatgcgaccg
     9901 ccctgccctt atttcaccat ctgacgagcc gcaatttgcg agcataaaaa tgcaccatca
     9961 acatcaacgg taaaaatctt gcgccactca tcaaaagcca tatcaagaaa cggcgctttg
    10021 gtcattgcac ccgcattatt caccagcaca tcaatacgcc caaatcgctg gatgagtgtc
    10081 tccagcgcct gcgcaccttc tggcagattg ccgagatcca gttgcacgat ctccgcacgt
    10141 acgccgtggg taaccacctc acgcgcggta tcttttgccc cttcttcatc tgagtgccag
    10201 gtaatgccaa tatcaaaccc ctgatgcgcc agtaataacg cgcactcttt gccgatcccc
    10261 gaatcggaag cggtaattat cgcgacctat gccatcgagt tctccactta acgctgaata
    10321 aacgttaagt atagaaggcg catatcatca gcgtttgtaa cccccgccca gcgcaccggt
    10381 gagtcgaatg gaggcatcca gccactgccc ttgcaataac aggccattgg cccgctcacg
    10441 cagcgccggg attctggctt cgctgacgcg ggaaccagca atgatgcccg cgttaaagcg
    10501 ggcctgcgca agacctacca cacgcaaggc atcgcgctca atttgcgcct gatgctggtt
    10561 tttctccgcc agtgtctgaa cctgactggc tgcccgcgcc acgtcattca ctgcttcaac
    10621 caccgctttg ttgtagctgg cgatagacaa gttgctttcg gcttttgcga tatcgagatt
    10681 ggcgttaaga cgaccactat cgaaaatagg tagcgtcagg cctgccgtaa cgcccatttg
    10741 ctgcgcggaa tgacggaaca gatcgcttaa gtgcaacgca tcctgttgca ggaaggccat
    10801 caggttgatg tcaggataaa atgccgcttt tgccgcatca atagtgctta acgatgactc
    10861 aacgtaccag tgcgccgcct gcaaatctgc ccgccgggcc agtaaggagt accccagttc
    10921 atcaggaagc tggcttgcca ctttcggcaa cgcgaccggt ttaagcttca atgacttagt
    10981 ctggtgattt gtgagtgcat ttaaccgtgc ctcaataatt ttcattttcc ccacgacatc
    11041 gttgagctgc tgccgggttt tgctggcatt aatatcggtt tccataccgt caactgaaga
    11101 agtaatcccg ttctgatata gctggcgatc ggtcgcgata atggtgttct gctctttttc
    11161 tatttgctgc aagaccgtgt ttaacgccgc ctgggtttgc cactcccagt acaggcgggc
    11221 tacgctgcca gccagcaatt ggcgggtttg ctcgcgttcc gccgcccgtg ctttaaccgt
    11281 acccaggcgg gcagtaacct ccgcccgatt ctttccccag atatcgagat gccagcccgc
    11341 cgttaagcca aaagttccgt tggtgtacca cggaccggtc gtacctgcgg ccggatcgtt
    11401 cagagcaaac ggccccatta gcccttctgc cgacattttt tgccgctcca tatccgccga
    11461 aaagtcgatc tgcggaccat cctgagtggc aactgccttc gcctgggctt cagctagctg
    11521 aatgcgctgt tcagccacct gcatatccgg tgcgttctgt agtgcattgt taattaatga
    11581 agtgagctga ttatcgtgat actccagcca ccattggctg tctggccaac catttttcag
    11641 cgccgtgggt aatgcggtgt caacttgtgc agcgggcgtt tgctggctta acgcctggcg
    11701 ggtttcatgc ataggcgcac acccggccag catcagtaac agcggaaaac aggcgatggc
    11761 tggataaaag gaatcacgat tcatggggga ataatcaggt aagaaacggt gcgcggagat
    11821 taccgtgtgt tgcgatatat tttttagttt cgcgtggcaa tacatcagta gcaataaaac
    11881 gacataccca gaaaaatata gactaagtaa atgatatctt ccgatttatc ttaatcgttt
    11941 atg
//
LOCUS       DAJTYF010000115        11837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTYF010000115 DAJTYF010000000
VERSION     DAJTYF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.115"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..1019
                     /locus_tag="OO590_003292"
     CDS             <1..1019
                     /locus_tag="OO590_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6638551.1"
                     /translation="ELEEKWLDLKRMEISANAFNRYESVARTMVPKIGGGRLVSSVTK
                     EELLYIRKDLLTGYQNSTKNKAAAKGRSVVTVNYYMTTIAGMFQFAADHGYLEANPFQ
                     GIKPLKRARAEPDPLTRDEFIRLIDACRHQQTKNLWSLAVYTGMRHGELVSLAWEDID
                     MKAGTITIRRNYTKLGEFTLPKTEASTNRVVHLIQPAISVLKNQAEMTRLGKQHHIKV
                     QLREYGRSVNHECTFVFNPQVVRKSKQVGFIYKVDSIGDSWETAIKRAGIRHRKAYQS
                     RHTYACWSLSAGANPSFIASQMGHASAQMVFNVYGAWMTDSNAEQIAMLNQKLADYVP
                     WSPK"
     gene            1032..1802
                     /locus_tag="OO590_003293"
     CDS             1032..1802
                     /locus_tag="OO590_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6638552.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            1855..2250
                     /locus_tag="OO590_003294"
     CDS             1855..2250
                     /locus_tag="OO590_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6638553.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2291..3034
                     /gene="cmoA"
                     /locus_tag="OO590_003295"
     CDS             2291..3034
                     /gene="cmoA"
                     /locus_tag="OO590_003295"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6638554.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3031..4002
                     /gene="cmoB"
                     /locus_tag="OO590_003296"
     CDS             3031..4002
                     /gene="cmoB"
                     /locus_tag="OO590_003296"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6638555.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4167..6596)
                     /gene="torZ"
                     /locus_tag="OO590_003297"
     CDS             complement(4167..6596)
                     /gene="torZ"
                     /locus_tag="OO590_003297"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6638556.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMAAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6621..7721)
                     /locus_tag="OO590_003298"
     CDS             complement(6621..7721)
                     /locus_tag="OO590_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6638557.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8109..8855)
                     /gene="cutC"
                     /locus_tag="OO590_003299"
     CDS             complement(8109..8855)
                     /gene="cutC"
                     /locus_tag="OO590_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6638558.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(8869..9435)
                     /locus_tag="OO590_003300"
     CDS             complement(8869..9435)
                     /locus_tag="OO590_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6638559.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9651..11384
                     /gene="argS"
                     /locus_tag="OO590_003301"
     CDS             9651..11384
                     /gene="argS"
                     /locus_tag="OO590_003301"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6638560.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            11561..>11837
                     /locus_tag="OO590_003302"
     CDS             11561..>11837
                     /locus_tag="OO590_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HCR6638561.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLN"
BASE COUNT         2934 a         3004 c         2865 g         3034 t
ORIGIN      
        1 aggaacttga agaaaagtgg ctggatctga aacgaatgga aatctctgca aatgcattca
       61 atcgctatga atccgttgca agaacgatgg ttccgaaaat tggaggcggc agactggtgt
      121 catcggtaac caaagaggaa ttgctgtata tcaggaaaga tttgctgacc gggtatcaga
      181 attcaacgaa aaacaaagca gcagcaaaag gacggagcgt cgttactgta aattattaca
      241 tgacgacaat cgctggaatg tttcagtttg ctgcagatca cggttactta gaagcaaatc
      301 ccttccaggg aattaagcct cttaaaagag ccagggcaga gccagatccg ttaactcgtg
      361 acgaatttat tcgcctgata gatgcttgcc gacatcagca gacgaaaaac ctgtggtcat
      421 tggctgtgta cacaggaatg cgtcacggtg aactggtctc cctggcctgg gaagatatcg
      481 atatgaaagc aggaactatt actatcaggc gcaattatac gaaactcggt gagttcactc
      541 tacctaaaac tgaagcaagt acaaacaggg ttgtgcatct tatccagccc gctatcagtg
      601 tcctgaaaaa tcaggctgaa atgacaagac tgggtaagca gcaccacatc aaggttcaac
      661 tacgtgaata tggacgttca gtgaatcatg aatgtacttt cgtatttaac ccccaggtgg
      721 ttagaaaaag caaacaggta ggttttatct acaaggtaga ttccattggc gactcatggg
      781 aaacagccat taagcgtgcg ggcatcaggc accggaaagc ataccagtca cgacacactt
      841 atgcgtgctg gtcattatct gccggagcaa acccaagctt cattgccagc cagatgggcc
      901 atgcaagtgc ccagatggtg ttcaatgtat acggagcatg gatgactgac agtaatgcag
      961 aacagatcgc aatgctgaat cagaagctgg cagattatgt cccatggtca ccaaagtgac
     1021 acaagagact tattaaaatc agtgggttag ccttcaatac ccgtcatgtt aactgcgtgg
     1081 agggcaacac cacgctttac gccctgccga aacccgaggt tgtcctgcgc tggcgtgaac
     1141 agaccacaga tgacttccgc ttctgtttta agtttccggc gaccatttcg catcaggcag
     1201 cattacgaca ttgcgatgat ttagtgactg aatttttgac ccgcatgtca ccgttggctc
     1261 cgcgcattgg gcaatactgg ctgcaactgc ctgccacatt cggcccacgg gagctgcctg
     1321 cgctttggca ttttctcgat tctctccctg gcgaatttaa ttatggtgtg gaagtccgcc
     1381 atccacagtt tttcgccaaa ggggaagagg aacaaacgct taatcgcggt ttacatcagc
     1441 gcggcgttaa tcgggtgatt ttagacagcc gcccggttca tgcatcacgt ccacacagtg
     1501 aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc ggtacatgct gtactgacgg
     1561 cgacaaatcc gctgatccgt tttatcggta gtgatgatat gacgcaaaac cgggaattat
     1621 ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac cactacgcct tatctttttt
     1681 tacatacgcc agatattgcc caggccccgg aactggtaca taccctgtgg gaagacttac
     1741 gtaaaacgct tccagagatc ggagcagttc cggctattcc acagcaatct tctcttttct
     1801 gaatttgcca cctatcatag acaggtgcca tcggccattt taaagggagt ttgtatggta
     1861 agcgcgctat atgccgtttt aagtgcgttg ttattaatga agttctcttt tgatgtcgtt
     1921 cgcctgcgaa tgcagtaccg cgttgcctat ggtgacggtg gttttagcga actgcaaagc
     1981 gctattcgca ttcatggtaa cgcggtggaa tatattccca tcgcgattgt gctgatgctg
     2041 tttatggaaa tgaatggcgc agaaacctgg atggtgcata tttgcggcat cgttttgctt
     2101 gctggtcgtc tgatgcatta ttacggtttt catcaccgtc tgtttcgctg gcgacgttcc
     2161 ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc tggcgaatct ttggtatatg
     2221 ccctgggagt tggttttctc cctgcgttag cgcacaatac gccactttct ttttcccgga
     2281 tttttacgtt atgtctcacc gcgacacgct attttctgcc cctatcgcca gactgggcga
     2341 ctggaccttt gatgaacggg tagctgaagt cttcccggat atgatccagc gttccgttcc
     2401 cggctattcc aatattattt ccatgattgg tatgttagcc gagcgcttcg ttcaacctgg
     2461 tacgcaggtt tacgatctgg gttgttctct gggcgcggcg acgctctcgg tgcgtcgcaa
     2521 cattcatcat gataattgca aaattattgc catcgacaac tccccggcga tgattgaacg
     2581 ctgccgtcgt catattaacg cctataaagc ccctacgcca gtagacgtca ttgaaggtga
     2641 tattcgcgat atcgccattg aaaacgcatc gatggtggtg ctgaatttta ccctgcaatt
     2701 cctggaacct tccgagcgcc aggcattact ggataaaatt tatcaaggac tgaacccggg
     2761 cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat gccaaagttg gtgaactgtt
     2821 gttcaacatg caccacgact ttaaacgtgc caacggttac agcgaactgg agatcagcca
     2881 gaagcgcagc atgctggaaa acgtgatgct gaccgattcc gtggaaaccc ataaagcacg
     2941 cctgcataaa gccggttttg agcatagcga gctgtggttc cagtgcttta actttggttc
     3001 actggtggca ttaaaggcag aggacgctgc atgatcgact ttggtaactt ttattctctg
     3061 attgccaaaa atcatctttc acactggctc gaaacgctgc ccgcgcagat tgctaactgg
     3121 cagcgcgagc agcagcacgg gctgtttaag cagtggtcca acacggtgga atttctgcct
     3181 gaaattaaac cgtatcgtct ggatttattg catagcgtaa ccgccgaaag cgaagagcca
     3241 ctgagcaccg ggcaaattaa acgcattgaa acgcttatgc ggaacctgat gccatggcgc
     3301 aaagggccgt tctcactgta tggcgtcaac atcgataccg aatggcgttc cgactggaaa
     3361 tgggatcgtg ttctgcccca tctttctgat ttaaccggac gcaccattct ggatgtcggc
     3421 tgcggcagcg gttatcacat gtggcgcatg attggcgcag gcgcgcacct cgcggtgggg
     3481 atcgacccta cgcaactgtt tctgtgccag tttgaagcgg tgcgtaaact actgggtaac
     3541 gatcagcgcg cacatttgtt accgttaggt attgaacagc ttccggcact gaaagccttt
     3601 gataccgtct tttcgatggg cgtgctttac catcgccgtt cgccgctgga gcatctctgg
     3661 cagttgaaag atcaactggt gaatgagggt gaactggtgc tggaaacgct ggttattgat
     3721 ggcgatgaga acacggtgct ggtaccaggc gatcgttacg cgcaaatgcg taatgtctat
     3781 ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt tgtagatatc
     3841 cgcattgtgg atgtgtgcgt taccactact gaagagcagc gacgcactga atggatggtt
     3901 accgaatctc tgtccgattt cctcgaccca catgatccga gtaagacagt tgaaggttat
     3961 cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt aaaggtctgg tattactgcc
     4021 ggatgcggcg tgaacgcctt atccggccta caaacccttg ctaattcaat ctattgcagg
     4081 gactatgtag gcctgataag catagcgcat caggcagttt tacgtttgca taacctcagc
     4141 gcccgtttcc gggcgctatt cacttcttac gcctgaacag caggcttatc aaatgccgtt
     4201 aacttcggtg cgttacccgt atatttttcg atatacacca gcgcagagtt accggcacag
     4261 gcatttgcca gctgcgagct ggggatatcc gccgttaaca cgttcgcact gccgttttta
     4321 cacaagccgt tttccagatc tggccatgca ccttcatgca cgcataccac gccttttttg
     4381 atcccgtcag tcaccaccgc gcctgtcaaa atctgaccac gtttgttcca gacacgcacc
     4441 agatcgccat tcgcaatacc aaatcgagca gcatcttcgg tgtgaatagt gatcggttca
     4501 cgatcggcaa ccgcatattt tttacgcagt tccgcatagt taagctgact gtgtaaacgg
     4561 tgtgccggat gtgcggtcag aagctgcaac tgcttctcgt cggcggtacc cttccactca
     4621 tcaggcgcaa gccaggttgg gtgtgccggg caatccttat agccaaattt ttccagcgtt
     4681 ttggagtaaa tctcaatttt gccgcttggc gtacccagcg catttttcac cggatcggcg
     4741 cggaaatcac cataacgaac gtactgttcg ttcttctcgc tgcggcgcat ttcgatcagt
     4801 ttattttgct gccagaaggc attaaacatt ggcatagtga cgcgttgcgc acgggcccct
     4861 ttctgagcgg catcatagaa aaatttcagc cacgccattt catctttacc ttcggtatag
     4921 atctcttttc cgccaggttt gagtaattcc gcaagatcgg caaacacgtc aaaatcgtta
     4981 cgcgcttcaa attgcggagc aacagcctgc ttcatcggca caatatgctg gttgctgtaa
     5041 tcaccggtca tcgtcaagtc attgcgctca aacgatgtgg tgatcggtaa tacgatatct
     5101 gcgtgtttgg ctgctgcggt ccagtagcat tcagaaacga cgatcatctc cggtttctgc
     5161 caggctttga tcagacgatt agtatcctgg tgatgggtaa agttaccgcc acccgcccac
     5221 cagatcattt tgatattcgg atacgtttgt tctttaccgt tatgctgata tttgccgcca
     5281 ggattttcca gcgcatcgac gatacgggca acaggaatcg ccgccattcc gccatcatca
     5341 gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac gccgccaaca
     5401 cgcatcgggt tgccaccatt ggagtaatga taagagaaac caaaaccgcc gcccggtgta
     5461 ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc agtgtttctg ttcaccatat
     5521 tgctggcgct gaattcccca gcctgccatc agaattgtgc gattcgccgc catcagttca
     5581 gcgagtttga ctatctgcgc ttccggtacg ccagtaattt ccgccgccca gaccgcgctc
     5641 ttcggcgtat tatcgctctt acctgtcaga tactcttcaa actgcggata cccggtagtg
     5701 tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct
     5761 aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc aggtggcatt atcgccaaag
     5821 aattcgatgg tttcggagcg gataggatca atggcaatca ctggtttgcc agattttttc
     5881 agctgatgga agtattccag cccttgctca tcggtactgc tccaggcaat ttttaaggta
     5941 ttcagcgggt tcattcccca cagcaccaca acctggctgt tttccagaat cagcggccag
     6001 gaggcctgct gttcatacac ctctacagaa ccgaccacat gcggcatgat cacctgtgct
     6061 gccccggttg aataatcgcc gctatgcccg gaataaccgc ccgccaggtt catataacgt
     6121 tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc gccagccgta agatccggca
     6181 aaaatggccg atggtccgtt agctttacga atacgctcat gttgctcatg aatcagtttt
     6241 aatgcctgtt cccagctcac ctgtacatag gtatcttcgc cacgaccttt cgccggttgc
     6301 agtggattgt cgagatagct ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt
     6361 acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg ttttcgccag cgcgcctgtc
     6421 gaagaaacaa tcttgccgtc cttcacttct acgctcatcg ctccccaacg tcccgcggtg
     6481 aggattttac cgcccttctc ttctgcccat gcggggagcg gtgctgccga tgtcaccacc
     6541 agcgctccag cggcaatacc gctgtgttta ataaattcac gtcttgttaa tgccataact
     6601 tcctccctga tcaacgagga tcactgtttc tcggtaatat ctttggcgtt gtactggaaa
     6661 taccgcgtta aaatgtccag ttcgttttcg ctcatgctgg ttcgtgcccc cattcctttg
     6721 gcaatggacg gccacgcatt gacggtgtaa tggtcggcgg caatagaggc atgacaacca
     6781 gcgcaatagg tatcgtcaag tttttcagcg tattgccata gcggtttacg gtccgctaat
     6841 gcgggatcgg taagcgcacc ctgtaaagac gcctgacgcc attgattgcc gtattcgtca
     6901 gcctgccatt ccccgtttac cgtgagcgcc ttgatacctt cttcacttaa tgtggctagc
     6961 gccagccgtt gacctgccgc caggtagagc gtgttttcac tgccctgcat ttgataaccc
     7021 tgcaacagaa cgatcggctg tttgccactg gcatcaacga cggtgagatc ggtaccagga
     7081 ttcacggtag ccagctcgcc tatgcgagaa gttttgaaag gataaatatg tgcgccatta
     7141 gtcactgaag tagcggcctg actttccagc tcatgcgccg cgttgtcatc catttttatt
     7201 tctggcggaa aatgggcaat gcctttatga caatcgatac aggtttcgcc gcccttttgt
     7261 gctttgttat gcattttctg cgcagattca ctttgcgagg caatatccat ggcatcaaaa
     7321 gaatggcaac tacggcacgt tgcagagtca gttgctttta attctttcca tactgtttcg
     7381 gccatttcct ggcgatgagt ttcgaactta tcgtcactgt ctattttgcc gctaacaaat
     7441 tcatgataaa tatctttaga tgcttttaat ttagcaaata aataatccat ccctgacttt
     7501 ggaatatggc aatcggcaca ttccgcacgt atccctttct ggttcgaaaa gtggacagtt
     7561 ccctgatatt cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa tgctgtatcc
     7621 gacgttttat gtaaggcttt ttgcgccagc aataacccgc cgccaccaac cacaaccgct
     7681 atcagcaaaa acaataaccc aatgcgtttt ttccctcgca tagattcctc taaataaatt
     7741 ccagaacacc gaaatcattg tataattaaa tacacaacga attaaccgct tgcataactc
     7801 ggcacaacac tgcctgaaat aatcgataaa gaatatgatt tattacaatg taatcattaa
     7861 ttgctaagga ataacccagt tgctatttag aatatatatt tatcagtttt agcaatttaa
     7921 atcccataat caatgtgaat ataaaaaata tttttagcac aacgctatgc gcactgtcac
     7981 ccgctcaaca gatcacggtt atcgttcgtt ttttatactg ctcagggata aaaaaaggcc
     8041 cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac
     8101 ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc
     8161 ccgtctacga tataacgcga atactcgtcc gcgtgttcat ctgatgacat ggacaatcct
     8221 tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc
     8281 actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc agccataatg
     8341 attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcgtct
     8401 gatttttgcc ctgatgtcag tacccgggca atgcccaatt ccgcaagatt attgagtgta
     8461 tataaagggt tagcgcacat atcgaaggcg cgatgaaaag tcactgccag cggaccggca
     8521 gcagccatta ttttttccat tcgtggcata tcgacattcc catcaacatc gagaacgccc
     8581 gtcaccagtc caggaaaacc taattcgcgg accgtgcgca catcctcaag aatggcggca
     8641 aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg atgcacaggg
     8701 atcgtcaccc gctggcgcac ggatttcagt ccccccaacg acggcgttaa gcccccctct
     8761 tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagtgcacat
     8821 tccatgctgt aacagcaaat ttccagtaat gccatttttg ctccttaatt acgctgactg
     8881 ctcgctggca acgatctctt caatggacca cggatgaaac ttaatagttg ttttgccatc
     8941 ggtgaccgcc agcgtcggat tcggtaatcg ctcatgctca ccttttggtg aactggtttt
     9001 aactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt
     9061 cagcgtttct ggatcacccg gcagaacgat ttcaatatgt tcccaacctt cgtgtgggta
     9121 acgtttttcc ccaggccacg gtagctccac attggaaaac tgccagtgcg caacctgtac
     9181 cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatttttt ctgacaaaag
     9241 ctcgccacac tgttcaaaac cgcgacgcca gcgctcagcg gtgacgtttt gatggcaacg
     9301 caaagaaatg tgatcggcag tcagcggagt gatatccaac cccagacggc gggaaagttc
     9361 atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga
     9421 ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc
     9481 cctgtgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata
     9541 cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct
     9601 ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc
     9661 aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt
     9721 gcgaaccgca gattcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca
     9781 tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga
     9841 ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca
     9901 acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc
     9961 gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg
    10021 tggcgaaaga gatgcacgtc ggtcacctgc gctctaccat tattggtgac gcagcagtgc
    10081 gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca
    10141 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa
    10201 tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg
    10261 aagagtttgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt
    10321 tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccaa atcacttacg
    10381 atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc tacaacccaa
    10441 tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg
    10501 cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca
    10561 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc
    10621 gttatgaaac actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac
    10681 acctgatgca ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc
    10741 tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg
    10801 cgggtggtac agtgaaactg gcagacctgc tggatgaagc cctggagcgt gcacgtcgtc
    10861 tggtggcaga aaagaacccg gatatgccag ctgacgagct ggaaaaactg gctaacgcgg
    10921 ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct
    10981 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgctt
    11041 acacgcgtgt attgtccgtg ttccgtaaag cagaaattaa cgaagagcaa ctggctgcag
    11101 ctccggtaat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg
    11161 aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtaatgtgt gcttacctgt
    11221 acgatctggc cggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa
    11281 acgaagaagt gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctgaagc
    11341 tgggtctgga tacgctgggt attgagactg tagagcgtat gtaatcgatt tttcgtgaga
    11401 gtgaagcctg atcagggtta gccgatcagg cttttttatt gccatctaaa tgtattctga
    11461 aaacggacat gccattgttt tctcactgtt gagtaagagg ccagaagcgt aatatccagg
    11521 cccaggaaaa cgataactgt tcaatttaag gaataccgaa gtgtttaaat ttcttgtatt
    11581 aacattaggc attatctctt gccaggctta cgcagaagat acggttatag taaacgacca
    11641 tgacacttca gccatcaaag attgttggca aaaaaattca gatgatgata ctgacattaa
    11701 cgtgatcaaa tcatgcctgc gacaagaata caatctcgtc gatacgcaat taaataaagc
    11761 ctatggtgaa gcttatcgtt atatagaaca agtgccacgc acaggtgcaa aaaaacttga
    11821 tactgaacaa cttaact
//
LOCUS       DAJTYF010000116        11788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTYF010000116 DAJTYF010000000
VERSION     DAJTYF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.116"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            202..537
                     /locus_tag="OO590_003303"
     CDS             202..537
                     /locus_tag="OO590_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6638562.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            630..713
                     /locus_tag="OO590_003304"
     CDS             630..713
                     /locus_tag="OO590_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638563.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            813..1634
                     /locus_tag="OO590_003305"
     CDS             813..1634
                     /locus_tag="OO590_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6638564.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="OO590_003306"
     CDS             complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="OO590_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6638565.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="OO590_003307"
     CDS             complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="OO590_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6638566.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2765..3799
                     /gene="tqsA"
                     /locus_tag="OO590_003308"
     CDS             2765..3799
                     /gene="tqsA"
                     /locus_tag="OO590_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6638567.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQLLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="OO590_003309"
     CDS             complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="OO590_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6638568.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="OO590_003310"
     CDS             complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="OO590_003310"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6638569.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7279..8223
                     /locus_tag="OO590_003311"
     CDS             7279..8223
                     /locus_tag="OO590_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6638570.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8409..9791
                     /locus_tag="OO590_003312"
     CDS             8409..9791
                     /locus_tag="OO590_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6638571.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9828..10550
                     /gene="folM"
                     /locus_tag="OO590_003313"
     CDS             9828..10550
                     /gene="folM"
                     /locus_tag="OO590_003313"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6638572.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10547..10882)
                     /locus_tag="OO590_003314"
     CDS             complement(10547..10882)
                     /locus_tag="OO590_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6638573.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPIFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11011..11754
                     /gene="rstA"
                     /locus_tag="OO590_003315"
     CDS             11011..11754
                     /gene="rstA"
                     /locus_tag="OO590_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6638574.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT         2995 a         3014 c         2834 g         2945 t
ORIGIN      
        1 gctttgtaag tagtataata gttaacccga tcaagactac tattattggt agctaaattt
       61 tccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt gtacatatcg
      121 ttacacgctg aaaccaacca ctcacggaag tctgccattc ccagggatat agttatttca
      181 acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta
      241 ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ccgcctttgc tgcagagact
      301 gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgccagcgaa aactacacat
      361 cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat
      421 aaaaatacga aagctgaaca gaaagcccct gaacaaaaag cgcaggcagc gaagaaacac
      481 gccgggaaac acggccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt
      541 ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta agcgggtaac
      601 gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc
      661 ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc tgactgtagt
      721 tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt
      781 ttaataatca taattaccca ccagagtgtg atatgcgtac aaccattgct gtagtgttgg
      841 gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg
      901 caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca
      961 cgacccaatc accgtgggat gcggttgggc aactggaaac ggccagcggc aatttatgta
     1021 cggcgacgct gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc
     1081 ctaagggtaa agcggataag gcggtagcgc tgcgttttgt gtcaaataaa ggtctttggc
     1141 gctatgagat ccacgacata gaaggccgcg ttgatccgac actgggaaag cggttaaaag
     1201 cagatgggga tggttggatt gttcctcccg cagctgcacc gtgggacttc ggattgattg
     1261 tgctacgtaa tcccccttct ggcattacac cgttgccgtt atttgaggga gataaagccg
     1321 cgcttactgc cgcattaaaa tcggctggtc gtaaagtgac tcaggcggga taccctgaag
     1381 atcatctcga tacgttatac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt
     1441 cggtgatgtc acatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc
     1501 ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc
     1561 gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag ctggatcaac
     1621 tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcgtctt catcaggcaa
     1681 gtttcaccat gatcattcca gccaacagca agaccaggcc aatccagcct ttacgattta
     1741 accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aacccgcccc
     1801 acaatgcata agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg
     1861 ccgccagcac cgccgccagg gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa
     1921 atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt
     1981 gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta
     2041 cctgatttga tcaacactat cccggcgacc agggtggtta acccggcaat tttcatcagc
     2101 gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accgatacct
     2161 tcccacagcg cataagctac gcctaaggcg atttttttaa cggcgaaaga gagaaatata
     2221 tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc
     2281 gcccatttca ttgacagcgt accggtaatt tctgtagcaa tagccagacc taataaaatc
     2341 caataaatat acattgtcct tctcctgcaa gagaactatt ttaattttcg cttaattcag
     2401 cgaaaccaaa gtaaagtggt caagccctgc gcatagggca agcttaggca gaagaaagga
     2461 ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag atagtggatg aggtacgaag
     2521 atgggtaaat gtagaaaata acgtcctgaa caaattgtcc ataatattac aattatccgc
     2581 agtgttgctt ctcgtcatcg cagatgataa ttgtcctcgg tagttgaaca cgcctgattt
     2641 gtatcatagc ttaagaatta actcaaaata ttttcacttc tttacctgag cggtttgatt
     2701 ttcgttatga tgacggagcg aaaaagacat tattattagc aaaggaagaa aaacggggac
     2761 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
     2821 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
     2881 cgcattattt attgctgtta ttcttaaccc gctggtgcaa catatggtcc gctggcgtgt
     2941 gccgcgtgta ctggcagtgt cgattttgat gacaatcatc gtgatggcga tggtgttgct
     3001 attagcttat ctgggttccg cgctcaacga gttgacgcgg acgttaccgc aatatcgcaa
     3061 ctctattatg acgccgctgc aagctcttga gccgttgttg caacgcgtag ggattgacgt
     3121 ctcagttgac caactggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa
     3181 cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc tgacggtgct
     3241 gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga tggcgcgtcc
     3301 ggttgaaggg atggcggcga ttcaacgtgc gattgacagt gtttctcatt atctggtgct
     3361 gaaaacagcc atcagcatca tcaccggctt ggtcgcctgg gcgatgctcg ccgcactcga
     3421 tgttcacttc gcttttgtct ggggattgct ggcctttgcg cttaattaca tccctaatat
     3481 tggttcagtt ctcgcggcaa tcccccctat cgctcagcta ctggtgttta atggcttcta
     3541 cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct
     3601 ggagccgcgc atcatgggac gtgggctggg gctttcgaca ttggtggtat ttttgtcgtt
     3661 gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat
     3721 tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag
     3781 cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga
     3841 ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca
     3901 gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct
     3961 gcgctttcca cacttccagc acaggcatac cagcaatcgg actcttcgga tcatcctgcg
     4021 ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt
     4081 catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca
     4141 atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacattaa
     4201 tactacgagc gcggagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg
     4261 ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct
     4321 cttctgcggt gatttcgcgg tgctcaccca cttcctgatc atcgccagta gaagagccgt
     4381 cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc ttacacataa
     4441 tgtaagaaag gatagccccc gaagaaccga ccagcgcacc ggtcacaatc agcaggtcgt
     4501 tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca
     4561 ccaccaccgg catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg
     4621 caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa
     4681 atacaatcag cagcaagaag gaaacgacca gagccgccag gttcattttg tgacggtttg
     4741 gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgagc
     4801 ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga
     4861 ccagaatcgg tgccattccc gcgtcatgat gcagataact gttaaagcca accagcactg
     4921 ccgccagacc cacgaagctg tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa
     4981 ctttcttcgc cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc
     5041 caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc atcccggcga
     5101 taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc agactgaaga
     5161 taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc
     5221 ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata
     5281 ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc tccctggcca
     5341 atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtaac cgacatcaac
     5401 ggtgtatgca gcgcgtgcga tacattccac accacgtaat aaccgacaac gcaggccagc
     5461 gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgacag ccaaccgaac
     5521 aggatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggc acatttttcc
     5581 tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc
     5641 ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca
     5701 tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt cagcagatta
     5761 acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta
     5821 taaccaatca ctttgacacc attttccgta ctgaagattt cacccggcac gatgtattca
     5881 cagttgccac cgttttgggc tgccaggtcg acaatcacac tgcccgcctt catggagtca
     5941 accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca
     6001 atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt gatgaacgcg
     6061 tccgacatca ctttggcata gccatcgccg ctgcccgctt cctcgttaaa atccagctcg
     6121 aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca
     6181 cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccaaacctgc aacaccggca
     6241 ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtgatttg cccggtaaag
     6301 aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat attcgccatc
     6361 gagcttagtg cgtccagcga ttgtgcgcgt gagattcgcg gcacagagtc catcgccatc
     6421 acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata
     6481 aaactcacca gcgttgtccc gggattcagt aacgcaattt catcatctaa cggcgcattg
     6541 accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct
     6601 tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg
     6661 gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca
     6721 ttggttaacc gttctcttgg tatgccaatt cgcatgatat tcccttccat cggttttatt
     6781 gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac
     6841 tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc
     6901 tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat
     6961 agtgggataa acaccttaga acgccggata aagactgata attgtcttcg acggtcgggt
     7021 aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact tatacaattc
     7081 agttgcttca gtagtaatga tgctgatacg gctgtttttt aagcatagac ggtcatttga
     7141 gcaggattaa aattggttta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt
     7201 ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca
     7261 aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct
     7321 gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt
     7381 aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa
     7441 gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt cgacacttct
     7501 gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa
     7561 aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc gaaagatcag
     7621 gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg tagccagcca
     7681 gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc tttctacatc
     7741 gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa
     7801 aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga ttccgaagca
     7861 ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga aagttgagat cccgggtgtt
     7921 gcgactaccg catcaccaag ttctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa
     7981 ggcgggcgtt acaccgtcac gctcccggat ggcactaaag tcgaagaact gaacaaagcg
     8041 acagcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg
     8101 accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac
     8161 atcacccgcc agtggcagaa acgtggtaat aacctgaccg tcagcgcaga tctgtataaa
     8221 taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa
     8281 atttccccgc gaccattgca tgcagacaca tcttttcgta aaatcccgcg cttcagtggc
     8341 aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt tttaatcact
     8401 ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa
     8461 tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag
     8521 cactgctcat cggctggggt attactggcg ctggcatttt attgctggcc tttgccatgc
     8581 tgatcctcac gcgcattcgt cccgaacttg atggcggtat ctttacctat gcccgcgaag
     8641 ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg
     8701 ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg
     8761 aattgcgcct gtttggcgat ggtaatacct ggcaatcgat agtcggtgca tcggctttat
     8821 tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc attaacctgg
     8881 tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt
     8941 tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta cctgtttggg
     9001 aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg
     9061 tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg
     9121 ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggt gtggtggcac
     9181 gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg gaaatgatgg
     9241 gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggcgcgtact
     9301 taagctggac catcatggcg gcagaagttc cgttcctggc tgccactcat aaagcattcc
     9361 cacgcatttt tgcgcgccag gacgcacaag ctgcgccatc cgcctcactg tggttgacca
     9421 acatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacgt
     9481 tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc gcgttcttgc
     9541 tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct
     9601 tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcac
     9661 cggggctgct ggttttcctg tatgcgcgca aaacgcatac acatgataat gtactgaacc
     9721 gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc
     9781 tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaaaccc
     9841 agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc ctcgcatggc
     9901 atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacacactat ccagccattg
     9961 atggactgat taatgcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg
    10021 tgatggcgtt tgccgatgaa gtactaaaaa ccacccatgg tctgcgtgct attttgcata
    10081 acgccagtgc gtggattgcg gaaaaaccgg gtgcgccact ggccgacgta ctggcttgca
    10141 tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctggaa agattactgc
    10201 gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg gtggagcgcg
    10261 gcagcgacaa acatgttgcg tatgctgcaa gcaaagcggc actggataat atgacccgct
    10321 cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctgatcc
    10381 tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa tcactgatga
    10441 aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt accagttgct
    10501 ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca tctgcgttaa tgcagcttta
    10561 tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa ccaacaaaag
    10621 cggcgggcaa gcgcatcatt ccggtgaaat accacagcga caccaggtaa acaaaatagg
    10681 gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc
    10741 tggcaacaat ataatgcgcg ataagcgcaa agatcgggaa aagtggaatc agcccggcga
    10801 tataataatt tttcgttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg
    10861 cggctttgat taccagcccc atctttttac cttaacactt ccataacaag tcatcagtag
    10921 aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat
    10981 tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt ggaagatgat
    11041 gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgta
    11101 gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag aaaatccgga tttggtgtta
    11161 ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag
    11221 tggtctggac cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca
    11281 ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgctagcg
    11341 cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgaccaaagg tcttcaggaa
    11401 acgtctctga ctccctacaa agccctgcat ttcggcacgt taactatcga ccccatcaac
    11461 cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg
    11521 tgggaactag ctacgcatgc cgggcaaatc atggaccgcg atgcattgct gaaaaattta
    11581 cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga
    11641 aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg taacaaaggc
    11701 tatctttttg cgcctcatgc atgggatagt gacaataaac aatcagatga ctaatatttg
    11761 ttcatctgaa aaagagaaca attaacga
//
LOCUS       DAJTYF010000117        11669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTYF010000117 DAJTYF010000000
VERSION     DAJTYF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.117"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(112..451)
                     /locus_tag="OO590_003316"
                     /pseudo
     CDS             complement(112..451)
                     /locus_tag="OO590_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
     gene            complement(767..1411)
                     /locus_tag="OO590_003317"
     CDS             complement(767..1411)
                     /locus_tag="OO590_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638575.1"
                     /translation="MMKQGMANSISLTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1432..1701)
                     /locus_tag="OO590_003318"
     CDS             complement(1432..1701)
                     /locus_tag="OO590_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6638576.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPEKLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1780..2418)
                     /locus_tag="OO590_003319"
     CDS             complement(1780..2418)
                     /locus_tag="OO590_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6638577.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2403..3635)
                     /locus_tag="OO590_003320"
     CDS             complement(2403..3635)
                     /locus_tag="OO590_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6638578.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHILEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            complement(4136..6817)
                     /locus_tag="OO590_003321"
     CDS             complement(4136..6817)
                     /locus_tag="OO590_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6638579.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVKRAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSLQLS
                     YYEKNIREKQISLNEYEEVFKKSDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFSAET
                     YRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(7058..8104)
                     /locus_tag="OO590_003322"
     CDS             complement(7058..8104)
                     /locus_tag="OO590_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HCR6638580.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSAGTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(8208..8357)
                     /locus_tag="OO590_003323"
     CDS             complement(8208..8357)
                     /locus_tag="OO590_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638581.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            8779..8970
                     /locus_tag="OO590_003324"
     CDS             8779..8970
                     /locus_tag="OO590_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6638582.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMRDGYPHPCM
                     QPMYNFLGKFKPEPREETE"
     gene            complement(9023..9256)
                     /locus_tag="OO590_003325"
     CDS             complement(9023..9256)
                     /locus_tag="OO590_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638583.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(9352..9975)
                     /locus_tag="OO590_003326"
     CDS             complement(9352..9975)
                     /locus_tag="OO590_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6638584.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(10064..10573)
                     /locus_tag="OO590_003327"
     CDS             complement(10064..10573)
                     /locus_tag="OO590_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638585.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            11031..11489
                     /locus_tag="OO590_003328"
     CDS             11031..11489
                     /locus_tag="OO590_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638586.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT         3268 a         2905 c         2336 g         3160 t
ORIGIN      
        1 catactcctc cggcagaagc agaaaaagct tattatgctt ccatcggaaa cgatgatctg
       61 gcagcctgag ttcacagata aaacactctc caggaaaccc ggggcggttc aaacctgatt
      121 ctggaaaagg actttgtgga ttgttgctat tgccagtgag attgaatgaa gtcgttttaa
      181 ctcgctttga atacgtcttg cccccagatt tcgctgagaa cgtaactcca gaatcagagc
      241 gacttcgcaa gtgccggttt tcgttgatgg tgaacgcttg gggcgacggc tgtggctttc
      301 cagaccagcg atgccctgag ccagatatct gcgccaccat tttacgcaat gtgggccgag
      361 aaatgccaca gcgacggcat acaaatcccg catcaccgga cgtttcatag agttttaccc
      421 actgtagccg ctgctgaatt tccctgttca taatttggca ttagttcagc tcaatcccga
      481 cttaaaaagt ccctacagga tgttccggac ctgaggcaca gttcatatcc ttatccataa
      541 aaaatttagc tatgtctata gcattaatct tgacaagacg acgttagggc ctggcgatga
      601 atcatttccc ctgccagata tcctttgggg tgcaccagaa ctaccgtttt atatctatat
      661 aactgaaacg tccctcgcca tccttcatat tttgatgtag taacaccatt cactttcatc
      721 taccccccgt tttattaaca tatagtaaaa atcacctcgc cgcattttat tcttttgaag
      781 taaaatcatt taccagttca cttaaagctc tcttttgttc atcatcatac agagattgaa
      841 tccggaattt aatctcttct gcaaccagtc ttgcatcggg ctcataaaat atttttgtta
      901 ttcgctgttt ctgatgttca ggctggtctt ccatatttcg taaaaccaga tgaaataata
      961 tatcataaag tgcgcaatgc catgaataac catcattggt taaaataagc tcccaaaccc
     1021 gctcctgctc ggcaataagc tgcagaacaa aggactcaac ctgttctgtt tgttcggggg
     1081 caacacggta tagcggagca atcacagcaa aagatagttc atttaaccag gattccgctt
     1141 ccttctcgct cctgaacggt ggacagtttt gtattcttcg ttcgtattca cgggtaatct
     1201 gctccagaat atcctccggc ggtgtcagcc acttattcct gagcaataca ctggttgatg
     1261 gatgacagct ggctatttca gcaataattt cacgtaatgt ttccgggctg aacttcttca
     1321 gctgattgta gtgctcccgg gtaaaaccat gttcatcgca taaaagttgc catgtcagtg
     1381 atatactgtt tgccattcct tgcttcatca ttaatacctg ttacctgaat atcaacagac
     1441 cggtgtacct tttctgaata cctccccgga cggagtggca atccccattc gcctcagcgt
     1501 ggcatctgca gctttcgcca cctcgctgat atccagcttt tcaggtagcg gcggggacga
     1561 tatctgcgcc agacaataat tccggcgcaa caacaaccac tcccgttgtg cttctgtaca
     1621 ttcaacattt cccataacaa aaagccagcg gtcagaagct ctgcgccagc aaccagcagc
     1681 ctccagtttc tgcgcaacca tgtcttccac aacttccggg gtactctttt tcctgctcaa
     1741 aacattaact cactgaaata atatacatat ctgacggtct cagcgaaccg tccatgccgg
     1801 gctgaaatcc tcttcctgaa acagctccgt cagcccgcta atctgtttca atctgagaac
     1861 ttcatcctgg tccataccca gctccgtacc aatgcgttca ttcgtccagc ccaggcgcga
     1921 caggtccctg acaatatcag acattgaggt tatctgatgt ttgcccctgg cccggttatg
     1981 tcggatggtc gcagcaatac gtgaggcctg tccctttctc tccggattaa tacagaccac
     2041 cggcagccag cccttcagac gttttcccgt acctgcttcc ctgcccagca cctggcgatg
     2101 aaaaccatcc accaccaggt aatggttctt ttcctcagac accaccaccg gttgtgtgaa
     2161 accatctttc tccagtgact gcttcagcag tttcttctca cccggcgcca tgacattagg
     2221 attgtaatca ttggccacca cctcatctgc ttttacccac agcacacagt ccactggctc
     2281 atgtgcaaaa ggactgactt catgaagcac tctgcgcagg tgattaaggg cctgtatggt
     2341 ctcttcttct ggtcgttcct ggctgaagta ctgacgaatc atgttgccaa ttatctccgg
     2401 tgtcacagaa tcccccattc cttcctcctt tctttcatac gctgcagata acgttcatag
     2461 tgccggggtt tgttcggact gaaggagagg gtccggcacc agaaatcatt ctttataagt
     2521 gtcttacaga tacgccgcca ggacggaata tcccggctcc ccaggtcatt ctcctgttca
     2581 tccgggatgt catccgggaa gccccgcgtc tgataccagc gcaggtagac agcaatttta
     2641 ttacggtaat gttctgccgt tctttccggc atcacatcca gcaggaacat cgcatagctg
     2701 cgccaggtat gatgggctgg ttttgatata cgtttacgca gggcaaaata ggcaccgctt
     2761 tcattggcat aaagcgcccc gctggcagcg cctgacaccc gctcacacat cctggcccag
     2821 gtttccggct ccagaatatg gtaaagccac agccccttac gttgttcagg accaaaaggt
     2881 tcacagacac gcatgttgcg taacggcacg tcggcacggt acatcaggtc atacagagga
     2941 ttgtaaatgg cacaagctct ggcgttatat atccagatat cgcgggcttt ccagtcatac
     3001 aacggataca tggtgtaata aaacccttca ggcgatgccg tggtccaggg cttatcatcg
     3061 gcatatctca gtttgcgctg agacaccagc cccataaagc gattgaggga ttcatccgca
     3121 cgaacaccgg tcagcactgc cactccacac cggttaccgg caaaccagga agaaaatgcc
     3181 ggaacaaatt cttcaaacgt catggcatac cggaaaaagg gaaaatatgt catatccgta
     3241 atggcctctt ccggtggctg gcgaacccag gtcactcctg gctcccagca tatccactcc
     3301 ggctgaaact gcgagacgcc gtttaccgtg gtcaggggaa gtgccaccca gtaaaaggtt
     3361 tccgtcacat catggtacat ttcccgcatc ttctgaatat gttcaatggt gcaccgatac
     3421 tgggcctccc agtcaatgaa cagaacagag aaatgacgtt ttctcctgcg ggccacctct
     3481 gccacaagat ggaaaagcac ggtggaatcc tttccacctg aaaaagacag acataccgac
     3541 ggaaatgttt caaaaatcca ctcaatgcgg gcgattgcgg cacgcaaaac attttctccg
     3601 gtaaggattt tttcggttaa aagcgtatca ctcattttcc tggctttatg tcgaataatt
     3661 ctgtttatac attcaggcaa acttcattaa taactcaccg ctttggggat aaataataac
     3721 aacaaaaatc ttcagatatt aacctggttg ttggatttgt tatcacatcc tttatccacc
     3781 tgcttcttac tggtaacaca tctggaaaat ccatgcattt tcaacatata ctatcctttc
     3841 aacttgtaaa taagaaaata tgatgcagat ggtgaacaat tcaaatatca ttttgcagga
     3901 agtattcact gtaatagaat taccccctga taaagtttat tcactaagga attattcaac
     3961 agaaacattc cctgttagct ttgagcaata gcacttattt ttatatgtat caggaatacg
     4021 tacgttgatg taaccaaaac aaagaagcct gtgtccatgg ggttcgtaca attgtgtgtg
     4081 gcacatgacc aaatcaggtt tgagacctcc cgctgcaata ttttttattc agattttagc
     4141 ctgtcaggcc tgacagaaac gctcccaact tcccttctat atcactgtct atagtacgta
     4201 atcttagcaa tggcaggccg catttcttca gtatactgtt tttaagttca tcacgttcag
     4261 cctgaacact ggtcagatga taacctccat ccacttcaat aacccctaat ggagtcttcc
     4321 cgaccttata gtaaacaaca aagtcacaac tggcccggtt acgcatgaat aactgttcac
     4381 ggtgtgtaaa atccccccgc tccagcaaca ccaactgatt cagcgctatt tgtgaatgaa
     4441 acatcatact ccgataacta tcctgcgaca ggatatcccg caacaggcag gccacaatct
     4501 gttctgactt aaagtgtgaa tcatttgaat tcaatctact attcagtcgt tccagtgatt
     4561 tgtcatactc actgtacagc aaatcaaatg ctgatatgac cgggctctcg aaaatctctc
     4621 cgtcatcagc gtagtatttg atgtaacgaa tcagcgccgc aatatgtcca gcgtggcgtt
     4681 caaatacatc attaccagtc accagcgtaa atttatttcg ggcccttgaa acagcgacat
     4741 tcaccagttc aggattatca acaaacgcga tgttccgact gtgctggctg ctgcgcttct
     4801 tatccagcac agtggaaaac acaatttcat cgcactcccg tccctgaaat ttatgcaccg
     4861 tcgatttcac aaaatcagca ggcagcactt tttcagccag attaacctgt gcattatatg
     4921 gggcaatgta tcccctgcta ctttcttcat cccagtaatg accttccaga gattcaagct
     4981 ccctgagatt tgaaaaatta cgggtatggt ttccttttgc cgtgatgacc agagataatg
     5041 aggcttcacc ggaatcaacc gttaacggta tcagagcatt atcataaaac tgcttattac
     5101 aaaattgaat gattttagga tgacaacgat aatgctcttt aagaagggtt actggcacca
     5161 tgttcctgaa caacatacaa accgaatcaa gtagactata tttctcacaa ttatagtatt
     5221 ctgccggtgg agatgggctg ttcgggagta atacaggaac atgtggaagt tgcttacgat
     5281 cccctacaac gattacatta cgggcacatc ccagtcccag aatgcccgga acaatatcct
     5341 gctgcgaggc ttcatcaata ataacataat caagaagggc ccccttgccg atagagttaa
     5401 taatcgaatg cgtactgcta ccaataatcg gaaaacgctt tataaaacgg tcaaattcat
     5461 ccctgtatgt ctctgccgag aatgaacttc ttgttgaaac atttcttcgc agatgttgct
     5521 ttaaataaag catagaccat gatgtcaggc gccccagtaa aattttaaaa tcagattttt
     5581 taaatacttc ctcatattca ttcagagaaa tttgtttttc ccggatattt ttttcataat
     5641 aagaaagctg aagagaagta aatagcgcca gccttctctc cggaatcatc aatggtttga
     5701 ctttcagtat catgaagttt aacaacaaat caattctgtt tctgaatcca atccgcctgt
     5761 cagaaaggta atgaatataa gccatcaggt cggttgtttt ttgtgaggaa aatcggtatt
     5821 tgtgagaaaa aagctcctcc ggtcgaagat tctgactctg ccattgctgc aggtatttat
     5881 actcaatatt cagctcatta atttctgctt ttaacgaagc gacctggtta atggcattca
     5941 ggtgtttttt cacttgttga agtacatcat caatcgcatt tgcagacggt gagtcaggta
     6001 agacttcctc agaacgcgaa atcgacgttg aaaaaaactg ttgcctgttc tccgtactcc
     6061 ccagtcgtgc catcacatat cccagttgct gtttgtccat cttttcataa acattactga
     6121 cagctgagtt attattggac aaaattgcca ctgttttatt ctgaattaat atattagcaa
     6181 caatattgag tatagtctgt gttttacctg ttccaggtgg tccttcaata actgaaatct
     6241 gtgaggaaaa tgcccgtttg acagccagca actgactttc attaagacca aacggaaaaa
     6301 tcaaacccca tggcgaatca cgttttttac tctgtcctga acagtatgca tgcaaggcgg
     6361 tatccggatg aggaactatt ttctttatct gcctaaggat attctcatcg atatttctgt
     6421 tattaccacg ggcataaaaa atacgttcat tagccaccct acacaagtaa ttaaacacgg
     6481 catgctctgt aaatgctgaa cactgttgaa agtcaaggcc ggtcgttttc atgatgtagg
     6541 gtcgttcacc ctcgcggtaa taaacagcag catatttatc accatatata ataaccagtt
     6601 cagcccggga tgttacagcg ttccctttcc tttcgaggaa aacatttcgc aaatttacag
     6661 ttggcgttat ctgacataca gaaagcggac gagtatattt tctcccgctg cgaaaatgac
     6721 aggtcagcat aacctctctg tcgtcatttc cagcccacag cgtccagtcg gtaattttat
     6781 gagtcttatt ttcaccgtca acacaaatcg tgaccatatt ctcactcaga aattatcatt
     6841 taacttacgg aaaattgtta aggcactccc gatattagct ccggaggatt ttccacaact
     6901 ccgggactgt taacatattt ctgcctgcca tatatacatt gaatttagtc ataacagtca
     6961 ataccagaat tacaggaatg cgatgccgat acgctggtta ttgaatactg gtagcccctg
     7021 agcactaatt catcacgata ctctgtattt actgttttca ctgcttttta caaccattcc
     7081 cccaggtaaa atgctgctcc tccggtgatg gctgtgtcag aaactgccat cccggaatag
     7141 ccacgctgac cgccagcaat gccgcacaga caccagccac aaacggtttc agaaacgtgg
     7201 gcggggattt ctgaagggag ggtgactgct ccgcctgcac tttccccttg cgattatcaa
     7261 ggctcagtgc atccagacgc accgcgccat ttgccgcaag cagcccttcc cggttatcca
     7321 cgcttcccgg cagactcagc gaaaaatccc ctgttcccgt ctgaatcaga ctgcctttac
     7381 gatttgacag gctttgtgcc gatatctgca gtgtgtcggc atttatcgta ccgccattac
     7441 tgctgaactg cccggcagtc cgggcggaaa gcgtaccggc actcagttgc gcattctgtg
     7501 tagacagatt tccactggtt gcattcagct cagtatgttg tccctgtatc cggctgttac
     7561 ctaaatccag cccctgctca atgtcccgta aatccagcag agaaccttcg tgtgccggga
     7621 atgaggaata caactgaata tagtcatcac tgtccggtgt cagacgcaca tgtcgcacaa
     7681 caccgggaat aatgaccagc cggaggatac cgctttttaa atcctgcgaa ggtgccagaa
     7741 cacgggtggt gacataacca tgatcgacca gacggttctg caatgtactc atcagcagat
     7801 taattccttt cgcccccagg caatgcccga ccgccccgtt ggcgattttt tgtaaaggca
     7861 gccagtgtgg taacgcatca gcccccttca gttccgtctg tttaatctga aaacagggcg
     7921 tttcaacagg aaaattaatc ttatgggcaa aagagccagg tgcagaaagg cgaacatcag
     7981 gggctgaagg cgtcagttgt tgctcaaggg cttcctgacg ctgctgctga atgataaatg
     8041 cttcatctcc gctacgccgg acatccgctg cctgaagaga taaaggcgat aataaagcag
     8101 acatcagtgt cagtgacagc aaggacggac agcgccggaa taagctttgt tcctcagtcc
     8161 tctcgttcct gcaacataat tttccagtca aacgaaaaac cttttattca tgtcatacca
     8221 acaatcacca gcctcctgac gatacctccc accatgtttt gcgatggcac gctaccgccc
     8281 ttccggtatt tacccgcatc aaccggtacc agaatgctga gtaacaaacc agccagaatt
     8341 gacttaattt tgatcattat agaataatga tctgaactac agcaacatga cagatttatc
     8401 gatcgatata acacaaatga acagttcttt attctgccat atatcgcaaa aacagcacat
     8461 tacaaaattt ataaccataa ttactggaaa agtgcagaaa attattccac tgaatgcttt
     8521 taatacagtt ggttagctta aaaaatagca atttttgatt tcagtcattg aaagttgcaa
     8581 tgtttgacga ttcaacagta tttaagtgag tttcaggata tgggaaggac atgaaaggag
     8641 gagcgcaata aaatacacag gaagaagggg aaaatgtttc gtaaaaacag aaaattaaca
     8701 gcgcacatca ggaaacgcag tctcctgatt cagtgctacc acatgaaaat ttagttaact
     8761 gttaccgtac taaaatttat gtcagaagat gttccattac cgaaagtcaa tcagcgatac
     8821 aaagacgatc atggtgcgct tgtcaccctg accagtgttg aggaaacccg ggtggtgttt
     8881 atgcgggacg gctatccgca tccctgcatg caaccgatgt acaactttct gggaaaattc
     8941 aaacccgaac cccgggaaga aacagaataa aacgaaatcc ctgccggcaa tcacactacc
     9001 tgatagcagc ataatccact tattaattct catccccgga gacaagccca gaaaaccact
     9061 ccgggtgttc actgtatgcc gtgttcagcg catccctgga aacgtttata taaaagctgt
     9121 cctgcaattt gcgggtaaca acttctgcca ggcgattccg gttatccacc agtccggcat
     9181 aacctctctc tgccagccac tcattcagtt catgttccag agacagattg ataatatcat
     9241 aatcggacct tgccattccc gtttccttag cacatgtttt caggcacggg gataaagtat
     9301 aaagaatgtc atactgctgc tgccagagca atattccccc tgatggttgt atcagagatg
     9361 gtatgccggt actttgcggg ttgttcccgt caggttatcg tgcacctcca cggtcgtggt
     9421 caccaccggc attccggcct ttctcagcct cagaacatca gccgcaatac gctgactgcc
     9481 aaaccagaac aggccgtcca gtgcagtcac cagcaaaccc gcctccagtg catacttcag
     9541 ccgttcacgg ggcgctttca cttcccgggc aacctgctga tatggcaatg atgtgttttc
     9601 attcccaacc acgcgacgaa tacggtgaga cagatgatac cggtatgtat ccggcacacc
     9661 agaaaggctg gccttcaggc tgtacacgca gccaaaccgt ttatcgttaa acaccacatt
     9721 tttctggctg atgccccatt cttcacgcag cgcggcaatc aattgcggtg tacgggtaag
     9781 cagcaagcga aaaggcagtt caaaactggt gacgtaatcc acattcagca gtgcaatacg
     9841 caaccgttct tctggcccgg cctctgtctg ccggcactcc tccagaacat cctgccactg
     9901 ccggcgaaga cggggggatt cgttccgttc tgtaaaacag tatttatccg ccagatagtc
     9961 aattcgtgta cgcatactga aaggaattcc gtataaagat tcagcagaca aaactttatc
    10021 agccagtaaa aattaacgga agcattgata ttcctcccga caatcaccgg atgattgttg
    10081 caataacctg cgacataaag gactgaacag ccgtttttaa cgccacgtat tgccctgatg
    10141 aaccaggccg ggcaacccga aaacatcttt ccacccggca ttgtccgcca gcacttatca
    10201 ccgaccaggt ctgttgcagt aaatccacca gttgcgcaca ttcttcatca gccatgacgc
    10261 cgactcgcgg atggcaaata ctcgtctggc tgagcagcaa cagacatcgt atctgtgctc
    10321 cttcatgttg ttcccgcaac cagcgtaata ccaccggcgc tgatgggctg accgtgtgat
    10381 tatgctccgt aatacgttcg tgtacccgtc ggtgaaagga actacaaaat gtctgaatct
    10441 gttgaccata gattccttct gccgaataga gcgtgaagtg aaatgccaac agatttggcc
    10501 agtggtccac cgcctgctga acaaaacgcc ggatttcccc cggctctgaa attaaggctt
    10561 cggttatttg cacgaaatgt tccttttcaa cccctgttga attttgtcaa gccggtgcag
    10621 acacagtaaa gcgcgcatca acacctgaaa ggtacgatac aaacacaagt tacaaacaat
    10681 aagaacggaa ggacaattaa tcagtgagga ataataacta aattaaccaa aagaatgtta
    10741 ctgacaatac taccaatact taaatagcaa tatattaatt aatgtaagaa cagatgcccg
    10801 gtaagacata tacacaatac atccacaaac acactctttt cattaaatca atcacctgat
    10861 taaaccatga aagtgtgatg taagtcaaaa ttactgatac tatcaattga taatgattat
    10921 ctctatcatt atcgtgaaat tcgagaatga aaggagaagt agtcattata agccttttat
    10981 aaatcgtcat catcatagta aagtagaaca ctttttatat gagttactat atgaaaatat
    11041 actggacaag aaaacgtatt cctgaacttt cagcacttcc accttcatta agaaagaaaa
    11101 attttactga cgcatataat gcagcatctt ctcatatcga atactggata ggtgctggcg
    11161 tctcatttat ctcgatgatg attttatcca gagtatatga ctttctttta cccgcccaag
    11221 atactttccc gggtgatatc atcagaagtt tatgtgttgt gtgtccatcc atattaatct
    11281 ggtttcagtt ttcggtatat gtcatgagga aatattacag acatattctg gtgcgaggaa
    11341 aagaaaccga aaccatttca gaaaggttga tacgtgaggc tgatacacga gaatatgaac
    11401 tatggcgtcc ggtccgtcgc tttttctcca ttgttttcct cattgttcta ttaggttgca
    11461 ttcattcact gataacaact ataaaataaa agttatggct gttctgatac aggaaaataa
    11521 acgttcattc tatggttacc tcttcctgtt atatagaaga attttaaacg acaagtaagg
    11581 gaatataaat gaaaataaca aaaaataaaa atgcaactat aaaaaacagt catcagtcgg
    11641 ctgcttttca atgtttacct tatcatttt
//
LOCUS       DAJTYF010000118        11478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTYF010000118 DAJTYF010000000
VERSION     DAJTYF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.118"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..594
                     /gene="phnP"
                     /locus_tag="OO590_003329"
     CDS             <1..594
                     /gene="phnP"
                     /locus_tag="OO590_003329"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR6638587.1"
                     /translation="GLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVGDPIPIY
                     GPPDDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHS
                     RVAWLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRS
                     PRVILTHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(741..1070)
                     /locus_tag="OO590_003330"
     CDS             complement(741..1070)
                     /locus_tag="OO590_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638588.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(1139..1771)
                     /locus_tag="OO590_003331"
     CDS             complement(1139..1771)
                     /locus_tag="OO590_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6638589.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGCCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(1852..4067)
                     /locus_tag="OO590_003332"
                     /pseudo
     CDS             complement(1852..4067)
                     /locus_tag="OO590_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            4181..5701
                     /locus_tag="OO590_003333"
     CDS             4181..5701
                     /locus_tag="OO590_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6638590.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            5698..6672
                     /locus_tag="OO590_003334"
     CDS             5698..6672
                     /locus_tag="OO590_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6638591.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            6703..7644
                     /locus_tag="OO590_003335"
     CDS             6703..7644
                     /locus_tag="OO590_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6638592.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            7699..8382
                     /locus_tag="OO590_003336"
     CDS             7699..8382
                     /locus_tag="OO590_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HCR6638593.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQRLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            8397..9257
                     /locus_tag="OO590_003337"
     CDS             8397..9257
                     /locus_tag="OO590_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HCR6638594.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            9254..10255
                     /locus_tag="OO590_003338"
     CDS             9254..10255
                     /locus_tag="OO590_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR6638595.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(10252..10958)
                     /locus_tag="OO590_003339"
                     /pseudo
     CDS             complement(10252..10958)
                     /locus_tag="OO590_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            11249..>11478
                     /locus_tag="OO590_003340"
     CDS             11249..>11478
                     /locus_tag="OO590_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638596.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILR"
BASE COUNT         2561 a         3175 c         3144 g         2598 t
ORIGIN      
        1 gggctgcacg atctcgccga tcgctggtcg cccggatcgt tccagcagtt tttgctgacg
       61 cattatcata tggatcacgc ccaggggctg tttccgctgc gctggggcgt gggcgatccg
      121 atcccgatct acggcccgcc ggatgaccag ggttgcgacg atctgtttaa acatccgggc
      181 ctgcttgatt tcagccacac agtggaaccg tttgtggtgt ttgatttgca ggggttgcag
      241 gtcacgccgc tgccgcttaa ccactcaaaa ctgaccttcg gttatctgct ggaaacggca
      301 catagccggg tggcgtggct atctgacacc gctggcctgc cggaaaaaac gctgaaattt
      361 ttacgcaata atcagccaca ggtaatggtg attgattgca gccacccgcc gcgcgcggat
      421 gcgccgcgta atcactgtga tttaaatacc gtgcttgcgc tgaatcaggt tatccgctcg
      481 ccacgggtga ttctgaccca tatcagccac cagtttgatg cgtggctgat agaaaacgca
      541 ctgccgtcgg ggtttgaggc ggggtttgat gggatggaga ttggggtggc gtgatgagag
      601 ggaatgtgcg cgctggtccc ctcaccctaa ccctctcccc agaggggcga ggggagtgat
      661 tgtgctcgat attgaatatt gcgctggttt tctccctctc cccattgggg tgaggggcga
      721 tgcctgctcc atacccaacc tcatcgccca tactcatctt ccattctccg ctcttcatct
      781 tctagctgcc gacgctcctg atcaagctgg cgctggcgat cgtccagctg cctgcggcga
      841 tcttcaaact ggcagcggcg gtcgtcatat tgtctgcgcc gatcgtcgct cacttcacgc
      901 tgccagccgt cgtcgcgcga atcttcatag tctcgcccac ggtcagagtt ataagcgtca
      961 ttaatcgcct gctgaatatt accaatagta tcgtcgataa tatcggcctg ggccggaacg
     1021 gtggacagcg tgagcagggt gaaaagaaaa agcgtggagt aaggtttcat aagccagtct
     1081 ctgcgatgga ctggcttaac gttagttgag tgtgaatgac cggagtagcg gagtaatctc
     1141 aaattcggta aggcgcagac tcttccatta gctggttagc tcgctgttta ttttccgcca
     1201 ccaatacaat gccatgacat ttttgatccg gtgaaatact ccagatacgc tcttccggaa
     1261 tgcagcagcc atcgtagatc actttcgtgc catcgtaggg ccaaaatgcc ccttgttcag
     1321 tgtctaatac aaacacggaa atctgatcgg actcgcgcgc gatcttctgc cagagcatct
     1381 tcccgccatc atattgatca aaatcgctga ttagcgtgat accgcattgc aggattagcg
     1441 cttcatacag ccgagttgcc agacccttat cgcgctcacc tggagagaca caaatacctt
     1501 ccacctgtac cagatccgag tcttcccacc agcgttcaac ggcaatgata tcgctgccga
     1561 actctttttt acgctgcaga tcgagagcgg caagtacccg atgacgcggc acgcctgcaa
     1621 aaacttcaat gtccgcttca atgatggccg ctcttggcgt ctggttgtca taaattacct
     1681 gtaacagacg tccagaaagc tcgatctctt ccaccacgcg aaaagaacga gcaaccgtca
     1741 tagggctata ggtggttggc atcacgatca tatgtaaatc acttcaattc agaacagaat
     1801 tgctcacaat agcagcacgt ttgcttcgag cctcttgata aattctgaaa atcatccctc
     1861 aatttgcccc agcacctgcg ccagctgcgc gcgggtaaag ggtttgcgca gcagcgccac
     1921 gtccggcagc gcggggttat ggctggggcg taaatcctga ccgctgatga gtagcagcgt
     1981 caggtgcggg taaagtttgc gggccgcatt gaccacttct gcgccgctca tgccgccagc
     2041 aggtccagcg cctgttcgcc gctgctcgct tccagtgtca aatagcccag caggtgcaac
     2101 tgctcgcaga tggtctggcg caccgcggct tcatcttcca gcactaacac cagtttatcg
     2161 ccattaacag ctgtctgttc taccgccgct ggcgataggt ttttcgccgc cgtcagcgcg
     2221 cggggaagct gtaatctgac cgttgtcccc tgccccggcg cgctttcaat ttccacgcgc
     2281 ccgccggact ggcgcacgaa accatagacc atcgacaagc ccagcccgct gccgctcccc
     2341 gtctgtttgg tggtaaagaa cggctcgaac acctggcttt tcaccgcctg cgacataccg
     2401 ctgccatgat cgatgacctc cagtgccacc atgtcctgac gctgcccact gctgcgggta
     2461 acacgctgat tccaggtacg aattttgata attcccgtct gcccttccat cgcgtcgcgg
     2521 gcgttcatca ccaggttgat gatggcgttt tccagttggc tgacgtcaat ccacgcggac
     2581 cacgccggag actgcgcttc aatctcaatc gtcagcgtcg ccggtaagga gtggcgcatt
     2641 aattcgctta actcttccag cagtgttttt agttcaacgg cttgcggatg taaggactgc
     2701 ttgcgggaaa atgccaacaa tcgctgggtc agtagcgcgc cacgttcggc ggcttgctgc
     2761 gcccgctgta ttcgcggcgc gtcgggtgag ttcgtgttca ccagttccag gctaccaata
     2821 attaccgcca gcagattatt aaaatcatgg gccagcccgc ccgtcagttg cctgaccgct
     2881 tttaacttct ggctgtgcaa tagcgcctct tccagcccct gccgctcggt ttgggcaatc
     2941 tccatctgcg tggttttctc tttcaacagc cgtgacgttc gttcaagtga tgcggtattg
     3001 cgggcaaaaa cgttaaaggc gcgcgccagt tcacccaact catcgcgccg ctgtaacgcc
     3061 ggaaaagtga cgtccgcttc gccgtgcgcc aggcggctca tcgcgcggga aatcgccgtc
     3121 aggttagagc cgagattgcg atagatatac caacctgcaa acgcggtgat caccaccgct
     3181 aacagggcga aaatcatgat cagcctctcg ccggagcgca aatcctggta actttgctcc
     3241 acccgctgac cagagagcgt cgccacctgt tcggcgtaac ggttgatgtc gccattaagg
     3301 atcgccacca gcgctttgat gtgaaacata taccagctga ttgccagatc gctttgctcc
     3361 agagccgccg aaagtggcgc gagtttacgc agttcacgat taaaatcagc gagtagcgta
     3421 ttaaccaatg gcgtatcgct gtgagttggc aaggcagaca tcactccgac cagttgatga
     3481 ataatcgcct gcggcgcggg ggtggcaatg gcggcaacaa tcagtcggtt catctcttta
     3541 agcaaaatat tatcttgcgc gtgagtgact ttttgcagat gccgcagata acttaagttt
     3601 tgatacagcg aacttaacag cgcgttacgc tccagatgcc gcctttgccc gcgttccagc
     3661 attccgccga cgctggtttg cagttcgttg ctacgcgtga tgatgcgcgt caccagcccg
     3721 gcttcctgct gcgctaaagg cgcgttcgcc agttgtgcaa gcgagctttt taatgcccgc
     3781 tgtgtctcct gcaaccgctc ggcttcgcct ttatattcca gtgctcccac cacctgcgac
     3841 aatcgcactg ccgccgttgc cacgtttgcc gtgtcgcgcg ccagattcag gctgccggtc
     3901 atatcatcta cagtttgctg cttcaccagc tcttgcatct ggctggcatg acgaaagccc
     3961 attaccgcca cgccgctgac catcaacgtc accgccacca ccagcaaatt caaaagcagc
     4021 aatcgcccgc gcgcgctggc aaaaaagtga cgtccggggt gcgccatatc acgctccgtg
     4081 aagaaaaatc gcaatatgac aaatatgaac ggcttctgac attttgcatt tactttcctg
     4141 tgatggagtg ctgatatcga caacctcgca ggagtccacc atgaacagca cgccggttct
     4201 tgagatgcgc aatattgcca aagccttcgg caaattttat gcgctcaaag gggtggattt
     4261 gacggtctac cctggcgaga tccatgccct gatgggggaa aacggcgcgg gaaaaagcac
     4321 gctgatgaag gtgctggcgg gggcgtatac cgccaccagc ggcgagatcc tgatagacgg
     4381 caagcccttt cacattcgca cgccaaaaga tgccttaagc gccggtatta cgctgattta
     4441 tcaggagatg cagctggcac ccaatttgac ggtggcagaa aatattttcc tcggcagcga
     4501 gctttcccac ggcgggctgg tgcagcgaaa agagatgctg gtgcaggcgc aaagagtgat
     4561 cgaccgcctc ggcgcgcagt ttaacgccag cgataaggtc atgacgctga ccattgccga
     4621 acaacagcag gtcgaaatcg cccgcgcgct acatcgcaac agccgcattc tggtgatgga
     4681 cgaacccacc gctgccctct cctcccgtga aactcaccgt ctttttgaac tgattatgcg
     4741 gctgcgcgat gaagggatgg cgattatcta catcagccac cgtatggcgg aagtgtatga
     4801 gctttccgat cgcgtcagcg tgctgcgcga cgggcaatac gttggcagcc tgacccgcga
     4861 taacctcaat gccggggagc tggtgcggat gatggtcggc aggccactga gcgatctgtt
     4921 caataaagag cgcgatatcc cgctcggtaa agcccgcctg aatgttcacc acctgaccga
     4981 cggcggcaaa gtccagccga gctgcctgca ggtgctttcc ggcgaaattg ttggcctcgc
     5041 cgggctggtg ggagccggac gttccgaact ggcgcagtta attttcggcg tgcggaaagc
     5101 gacaggcgga atgattgaag tcgatggcga accggtggtg atccactccc cgcgcgaagc
     5161 catcgatctt ggcattggtt ttctcaccga aaaccgcaaa gaacaaggct tattccttga
     5221 aatggcggca gtcgaaaaca tcactatggc aaccctggag cgcgatgcca gctggggaat
     5281 gctcaatcgc aaaaaagcgc aaaccatttc cgatgacgcc attaagttgc tcaacattcg
     5341 cgtgcctcat gcccaggtac gcgcgggcgg gctttccggc ggcaatcagc aaaaactgtt
     5401 gatctcccgc tgggtggcga ttggcccgcg cattttactg ctcgatgaac ccacccgcgg
     5461 cgtggacgtt ggcgcaaaaa gtgagatcta ccggatcatg aacgagatgg cgcgcaaggg
     5521 cgtggcgatc ctgatgatct ccagcgaact gccggagata gtgggaatga gcgatcgcgt
     5581 ccatgtgatg cgcgaaggca gcattgccgg tgagctaaat ggcaaaaaca tcacccagga
     5641 aaacattatg accttagcga ctggcgtcaa cgacgcccat tcccaggcgg tgacctcatg
     5701 acgacctctg caaaaacctc tgccaaaaaa atgctgatga gcgatctgat gcaaacggtc
     5761 ggcattttgc ccattttgat cctgatcgta gcggtatttg gctttatcgc gccaaacttt
     5821 tttaccgaaa gcaacctgct aaatattacc cgccaggcgt cgatcaacat tgtgctggcg
     5881 gcggggatga ccttcatcat tttgaccggt gggatcgatc tttctgtagg ttcaatcctc
     5941 ggcaccacgg cggtggcggc aatggttgtc tcccttagcc cggagttcgc catgttgtct
     6001 atcccggcgg cgctgatgct cggcctgctg ctggggctgt tcaacggcgc gctggtggcc
     6061 tttgccggat tgccgccgtt tatcgtcacg cttggcacct atacggcgct gcgcggggcg
     6121 gcgtatctgc tggctgatgg caccacggtt atcaactcca acattaattt cgagtggata
     6181 ggcaataact atctcgggcc ggtcccgtgg ctggtggtga ttgcgctggc ggtgatcgtg
     6241 ctgtgctggt ttattctgcg tcgcaccacg cttggcgttc atatctacgc cgtcggtggc
     6301 aatatgcagg cggcgcgtct taccggcatt aaggtctggc tggtgctgct gtttgtttat
     6361 ggcatgagcg gcctgctttc agggcttggc gggattatga gcacctcgcg gctgtacagc
     6421 gccaacggca acttaggtat gggttacgaa ctggatgcca ttgcggcggt gatcctcggc
     6481 ggcaccagtt ttgtcggcgg catcggcacc atcaccggca cgctggtggg ggcgctaatt
     6541 atcgccacgc tcaacaatgg catgacctta atgggcgtct cttacttctg gcagctggtg
     6601 atcaaagggg cggtgatcat cattgcggtg cttatcgaca aataccgtac ccgacatcat
     6661 caaagtgcat aacaacaata ccctacacat gaggaataca gcatgagatt gaagccctta
     6721 gtgaccgcgc tctgtgcagg cgcgctgctg gccgcagcac cgttcgccca ggcgaaagat
     6781 ctgaaatcga taggcgtaac ggtaggtgac ctggcgaacc cgttcttcgt acagatcacc
     6841 aaaggcgcgg agctggaagc acgcaaactg gcgggcgata acgtcaaagt gacgctggtt
     6901 tccagcggct acgatctggg tcagcaggtc tcgcagatag acaactttat cgccgctaac
     6961 gtcgatatga tcatccttaa cgctgccgat tcaaaaggga tcggcccggc ggtgaaacgg
     7021 gcaaaagacg ccgggatcgt ggtggtggcg gttgacgtgg cggcagaagg tgccgatgcc
     7081 accatcactt ccgataacac ccaagcaggc gaaatggcct gtaaatacat caccgatcgc
     7141 ctgaaaggca aaggcaacgt ggtgatcatc aacggaccgc cggtgtccgc cgtgcaaaac
     7201 cgcgtcgaag gctgccagac cgaatttaaa cgccatcctg acatcaaggt gctctctgac
     7261 aacaagaacg ccaaaggcag tcgtgagggc gggctggagg tgatgaccgg gctgctggcg
     7321 gcaaatccga agatcgacgg cgtatttgcg attaacgatc cgacagcgat cggtgccgat
     7381 ctggcagcaa aacaggcgca acgtaacgag ttctttattg tcggcgtgga tggaagcccg
     7441 gacgccgaag aggcgctcaa gcgtgagaac tcgttgtttg tcgctactcc ggcgcaagat
     7501 ccacaggtga tggcagcgaa agcggttgag atcagttatg acattttgca gggcaaacct
     7561 gcgccgaaag agccggtgct gatcccggtg acgatgatcg ataaaaacaa cgtcggcaat
     7621 tacaaaggct ggacggtgaa ataagtccac acgccgggcg gcgatgctgc ccggccaccg
     7681 ctaaggagaa cgctttcgat gaaacgctcc gctataaaca acattctcgg ccatacacgg
     7741 caattctttt cgcaacatga cgtacatctg ccgccctttg ccagtttctc tcctgctcaa
     7801 tggcagcagc tcgacaccgc cgcgtgggag gtggtttttg atctcaaact cggctgggat
     7861 gttaccgctt ttggtggtaa caactttgca gcacagaggc tgacgctgtt taccctgcgt
     7921 aacggctcag caaaaggaat gccgtatgtg aaatgttacg ccgagaagat catgcatgtg
     7981 cgcgatgcgc aggtcacgcc aatgcatttt cactggcgta agcgtgagga catcatcaat
     8041 cgcggcggcg gcaatttgat tgttgaactg tggaatgccg atagcaacga gcaaacagtt
     8101 gataccgacg ttacggtggt gatcgacggc tgccgccaga ctcatgtcgc aggcagtcag
     8161 ttacgcctgt cccccggcga aagtatctgc ctgccgcccg gcctgtatca cagcttttgg
     8221 gcggaaaccg gttttggcga tgttctggtt ggcgaagtct cttctgtcaa tgacgacgac
     8281 cacgataacc actttttaca accgctggac cgctacaacc tgattgacga agacgaaccg
     8341 gcccagctgg tgttgtgtaa cgaataccgc cagttcctct gaataaggat aacgttatgc
     8401 cgttgatttc tctcgctgaa gggctggcac atgcccgcga acatcactat gcgttaggtg
     8461 cgtttaatgt gctggactca cactttttac gcgccttgtt tgctgctgcc agacaagaac
     8521 gttcgccctt tattatcaac attgccgaag tacattttaa atatatctcg ctggattccc
     8581 tggtcgagac agtgaaattt gaagcggcgc gtcaagcgat tccggtggta ttaaatctcg
     8641 atcacgggct gcattttgaa tcggtggcgc aagcgttgcg gctgggattc agctcggtaa
     8701 tgtttgatgg ctccacgctg gggtatgaag agaatatccg ccagacgcgg gaagtggtga
     8761 aaatgtgcca tgccgttggc gtatcggtag aagctgaact gggcgcggtg ggtggtgacg
     8821 aaggcggtgc gctttacggt catgccgatg aatctctgtt taccgatccg cagctggcgc
     8881 gtgattttgt cgaccgtacc ggcattgacg cgctggccgt ggcgattggc aatgcgcacg
     8941 gcaaatatcg cggcgaaccg aaactcgatt tcccgcgcct cgacgctatc cgccagcagg
     9001 cggctatccc gctggtatta cacggcggtt cagggattag agatgccgat ttccgccgcg
     9061 ccatcgaact gggtatccac aaaatcaatt tctacaccgg catgtcacaa gcggcgctgg
     9121 ctgccgttga ggcccgaatg gcgcaccgtc agccgatgta tgacgagttt gccgagttac
     9181 tactagcagt ggaagccgcc atcagcgata cggttgcaca acagatgcgt attttcggca
     9241 gtgcggggca ggcatgatgg aacgtaaagg gattatcgcc gcaggcaata tgctggttga
     9301 ccacgtccac cagattatcc agtggccaga gcgcggctgg ctggcggaga ttacccacag
     9361 cgaacgctcc accggcggcg cgccgcttaa cgtcctgctg acactggcga aaatgcgcac
     9421 cgggttgctg ttacaggcgg tcgggctaat cggtcaggac agcgacggcg attacattat
     9481 ggcgatgctt gagcaatatc acgtcaatcg tcagcatgtg cagcgcacta cctttgcccc
     9541 gacgtcaatg tcgcaggtaa tgactgatcc aagcgggcag cggacgtttt tccattcgcc
     9601 aggtgcaaac agacttttgg atcttccggc gttcgatcag ctcgataaca ccatgaagat
     9661 tttccatctc ggctatttac tgttgctcga cagcctggat atgccggacg atgtttacgg
     9721 cacccgtagc gcacgcctgc tggcgcaaat gcgtgatact ggctttgaaa cctcactcga
     9781 cctggtctcc cgcaagggcg atccgcgtta tcagccgctg gtgttgcccg cgttgcgcca
     9841 tctggattac ctggtaatta acgaactgga ggccggggag tttagcgggc tggaaattcg
     9901 gttacccaac ggcgaaccgc atatcgcgca tattgctgcc gccgcgcgtg aactgttaga
     9961 tgcgggcgtg cgccagcgtg tggtgatcca ctgcccggaa ggggcgtggg gagagacccc
    10021 ggaacagtgc ggcgtgtgga tcccttcgca aaagttagaa caacgggaaa ttatcggcag
    10081 cgtcggtgcg ggcgatgtat tttgtgccgg atttttatat ggctgccatg aacgctggac
    10141 gcttaccgag agcatcaaac tggcacacgc ctgcgcgcga gcaagcctgt tatgcgccaa
    10201 tgccatcgac ggggcgaaaa cgctggaaga gctgcaaaca gaaatgtcgg attaatgcac
    10261 gtcggcagcg aagacatacc ccagaccgcg cagagtttta atcaacatcg gctgatgcgg
    10321 gttgagttcg attttgcgtc gtaagcgcat gattaatacg tcgatggtgc gatcaaacac
    10381 ctccacgctg tcgctatgag tgaacgccag cagctgttcg cggcttaata cccgacgggc
    10441 attttgagcc agggccagta acaagccata ttcgccctga gtcagctcga catgttgttg
    10501 tagcggacta tgcagttggc agcgaatggt atcgagatac cagccattga atgacagccc
    10561 ggcgtttttc tcctcattaa ccgccagcgc gccaacccgt cgcaacacgc tttcagtcgt
    10621 gcgaccacca cccgtggggt gaacggcttg cagatgtaat catccgcgcc catttccagc
    10681 ccgaccacca catcagactc actccccatt ccggtcaaca tcaccacagg caatgccgga
    10741 cgcagtttct ggatttgctg caataccctc aggccgttgg tatcgggcag catcatatcc
    10801 agcaccacca gcgcaatgtc aggctcgccc tcaatacgcg atatggcctc ctgtccggta
    10861 tggctgactg aaaccgtaaa gacatgttca cttaacacgt cctgcaacag tgcgcagata
    10921 gccgtgtcat catccaccac cagcacgacg ggtttcatgg ttacactccg catcattcat
    10981 ctaattaatc attgtgcgta cttcttgtcg aagtgaaagc ggaaagtaat ataaataata
    11041 aaaatataag tcaggtcaca ttattgatga tagtaataaa tatgccgtgc ttttccctct
    11101 tcagcacaaa tgcctaataa aatggcgcgt tccgctcaca cttccactta ttaaagtcgc
    11161 aaattcccaa ttatgataac cccagcttta aatgacgtat tttgcatgga ttacatattt
    11221 acgacacaaa tgataaatgg agatttatat gaataactct cggttattcc gtttgagcag
    11281 aattgttatt gcgttaactg ccgccagcgg catgatggta aataccgcta acgcgaaaga
    11341 ggaagcgaaa gccgccactc aatataccca acaggttaat cagaattacg ccaaatcatt
    11401 accgtttagc gatcgtcagg attttgacga cgctcagcgt ggatttatcg ctccgctgct
    11461 ggatgaaggt attctgcg
//
LOCUS       DAJTYF010000119        11426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTYF010000119 DAJTYF010000000
VERSION     DAJTYF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.119"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            8..451
                     /locus_tag="OO590_003341"
     CDS             8..451
                     /locus_tag="OO590_003341"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004133821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6638597.1"
                     /translation="MQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQIDES
                     RWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIKSES
                     ELTVDASITAKPFFERYVFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            697..1741
                     /locus_tag="OO590_003342"
                     /pseudo
     CDS             697..1741
                     /locus_tag="OO590_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1803..2417
                     /locus_tag="OO590_003343"
                     /pseudo
     CDS             1803..2417
                     /locus_tag="OO590_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2474..3931)
                     /gene="pepD"
                     /locus_tag="OO590_003344"
     CDS             complement(2474..3931)
                     /gene="pepD"
                     /locus_tag="OO590_003344"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6638598.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4193..4651
                     /gene="gpt"
                     /locus_tag="OO590_003345"
     CDS             4193..4651
                     /gene="gpt"
                     /locus_tag="OO590_003345"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6638599.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4743..5987
                     /gene="frsA"
                     /locus_tag="OO590_003346"
     CDS             4743..5987
                     /gene="frsA"
                     /locus_tag="OO590_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6638600.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6045..6446
                     /gene="crl"
                     /locus_tag="OO590_003347"
     CDS             6045..6446
                     /gene="crl"
                     /locus_tag="OO590_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6638601.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6485..7540)
                     /gene="phoE"
                     /locus_tag="OO590_003348"
     CDS             complement(6485..7540)
                     /gene="phoE"
                     /locus_tag="OO590_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6638602.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7828..8931
                     /gene="proB"
                     /locus_tag="OO590_003349"
     CDS             7828..8931
                     /gene="proB"
                     /locus_tag="OO590_003349"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6638603.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8943..10196
                     /gene="proA"
                     /locus_tag="OO590_003350"
     CDS             8943..10196
                     /gene="proA"
                     /locus_tag="OO590_003350"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6638604.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10311..10386
                     /locus_tag="OO590_003351"
     tRNA            10311..10386
                     /locus_tag="OO590_003351"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10344..10346,aa:Thr,seq:cgt)
     gene            complement(10401..>11426)
                     /locus_tag="OO590_003352"
     CDS             complement(10401..>11426)
                     /locus_tag="OO590_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6638605.1"
                     /translation="WRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLH
                     FREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKT
                     RHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVV
                     MFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQG
                     LHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWAS
                     WLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEEN
                     VVYLNAR"
BASE COUNT         2741 a         2837 c         2945 g         2903 t
ORIGIN      
        1 gaataacata caaataagag attatcagcc tggcgatttt cagcaactat gcgctatttt
       61 cattagagcg gttacgatga ccgtcagtca gcattattca ccacaacaaa ttgccgcctg
      121 ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag tgcgggttgc
      181 agtcattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata tcgatatgtt
      241 atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa aaccttttat
      301 taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct tttttgaacg
      361 ttatgttttt cagacagtta agcagcagcg cgttgaatgc cggggagcgt ggtttattaa
      421 tttttatatg cgttataaac cgcaacatta aatccagctt gcaatgaaaa taacgcccgc
      481 ctagtatgtg cgcgttttct tatttcacaa actgcaagga ggtaaatcat gacaaatcct
      541 ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg ccctggattc
      601 gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc agcacatgca
      661 acttgaagta tgacgagtct atttacggaa tggtatatgg gcaattatat tcgtccctta
      721 tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt agcgatccaa
      781 caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat gccagattta
      841 caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg tttttaccca
      901 gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga tattctcgct
      961 cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga cgttgctgaa
     1021 gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg gcgcagctcg
     1081 cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga tcaaatcatc
     1141 aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact gctggttcat
     1201 agcggctcga gggagctagg gcagtctatt ttacagcggc atattgcctc gttttcgcat
     1261 catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa tcgccacctg
     1321 atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct ggatgtggcg
     1381 cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca tcgtaaaggt
     1441 gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga ttactccagg
     1501 ctggttcagg cccgtcagga gtgaggaaac attgcattcg ctggcgcatg gggctgggcg
     1561 taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacag cgacgcaact
     1621 ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca tcttcgaaga
     1681 agcgccacaa gcctataaat cggctgaaag tgtggtgcaa tgtctgggca ggccggatta
     1741 attattcctg tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg agggaaaaaa
     1801 ggatgatctt gctataactc tcctcagcgc aggggccgga agaatgttgt ctcgcagtga
     1861 aaaaggcgct ggacaggcta attaaagaag ctgcccgaca agacgtcgcg gtaacggtgc
     1921 tggaaacaga aacaggccgc tactctgaca cgctgcgttc ggcgctgatt tctctggatg
     1981 gcgacaacgc atgggcgtta agcgaaagct ggtgtggcac tattcagtgg atttgcccga
     2041 gcccgtatcg cccacatcac gggcgtaaaa actggtttct gggcattggg cgttttaccg
     2101 ctgatgagca ggagcaatcc gatgcgatcc ggtatgagac gctgcgttcg tcggggccgg
     2161 gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatttg gcatccggta
     2221 ttagcgtgaa ggttcagtca gagcgcagcc agcatgctaa caaacggctg gcgcgattgc
     2281 tgattgcctg gaagctggat cagcagcaat aggaaaatag cgcggtgctg aaatcacagc
     2341 ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt acagggatgg
     2401 cttttatcga aggataatga aggatgaaac tgccggaagg cgattaaacg ccatccggca
     2461 gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc agtgtccagt
     2521 aatgacctac gctttcgatg tgaacttgct catccggaga gtgtggaccg gtgatagttg
     2581 gcccgataga aaccatgtcc atttccggat acggtttttt gaacagacca cattccaggc
     2641 ccgcgtggat aatctggatg ttcggcgtct tgttgaacag gcgctgatag gtttcacgta
     2701 ccagatgcat caccggagaa ttagcgtccg gctgccagcc aggatatgcg cctttcgctt
     2761 cggttttcgc gccagccagt ttacccagcg aatccagcat gctcaccacg tagtctttac
     2821 cgctgtcgat cagtgaacgg atcaggcagt gaatttctac gttattgtca gtcatggtca
     2881 ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggaa ttgcggatca
     2941 caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc gcaatcaggg
     3001 cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct ttctctgcca
     3061 gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagtgcg tcgactttat
     3121 cagctgtgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc agtgtgccgc
     3181 cgttgaaatc gataaggcgc aggtccagtt cttctgcatg acccgccagg aagcgcacca
     3241 gcagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga ccgcctttca
     3301 gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca cgatctaaat
     3361 gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca ccttcttctt
     3421 cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag ccgaacgcac
     3481 cgtccatacc ggcttcttcg gttatggtca gcagcacttc cagcgggccg tgaaccacgt
     3541 tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc gcacccagcg
     3601 tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga tctttcgtga
     3661 agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc tgtaagacga
     3721 ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata ttacctacct
     3781 gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat tcagcgagtt
     3841 gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata tcccacagcg
     3901 gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat cacccgcaaa
     3961 acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca ggcttgcgag
     4021 atatgtattg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
     4081 ggactcatgt gaaatactgg tttttagtgc gccagatatc tataatctcg cgcaacctat
     4141 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
     4201 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
     4261 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tggtaccggg
     4321 ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     4381 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     4441 cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga ttcgtgaaat
     4501 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     4561 tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg atatgggcgt
     4621 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcat tgccgggcgt
     4681 tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg gaggctgcat
     4741 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     4801 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggctcttg
     4861 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     4921 gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgctgaac
     4981 gtaccgacga tgatttatac gataccgtga ttggctaccg tggtggcaac tggatttatg
     5041 agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tacggaagaa gatccgcaac
     5101 tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc tatcctcatc
     5161 tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc tatgaagagg
     5221 ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc ggcggtgcgc
     5281 ccatcaccgg ctttttgcat atgccgcaag gcgatggccc gttcccgaca gtattaatgt
     5341 gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt tattttgcgc
     5401 cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct tcaaaatgga
     5461 agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg cctaacgtac
     5521 cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct aacgttgccg
     5581 tgcgtctggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt ggtccggtag
     5641 ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg tatcttgacg
     5701 ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttacgc gtggagctga
     5761 atcgctattc attaaaagtg caaggattgc tggggcgtcg ctgcccaacg ccaatgttat
     5821 caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta atcacctcat
     5881 catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg aattttgaca
     5941 aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa tttgctaaat
     6001 tttgccaatt tggtaaaaca gttgcatcac aacaggagat agcaatgacg ttaccgagtg
     6061 gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat attcgtgaag
     6121 gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc aacgtgaaac
     6181 cggcaccgga agtgcgtgaa ttctggggct ggtggatgga gctggaagcg caggaatccc
     6241 gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg aagagtgttc
     6301 cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag tttcacgaga
     6361 agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcggat gattttcgtg
     6421 atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca tccggcaata
     6481 ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc atcattatta atattcagct
     6541 tattatcgct atccagtttg ttgattttat aatcaacaaa cgctgacata tttttgttga
     6601 aataatacgt agcaccgacg tcgatataat tgaccagatc ttcatcaccg atgccctcaa
     6661 tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag tcaaactggt
     6721 attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt attggcgtca
     6781 ttttgcgtgt ttcagaatag aaagttgcca gataaatatt attggcatcg tatttcagac
     6841 ctgttgccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc tgctcgttgg
     6901 tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca aagtcatatg
     6961 tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt tcgtttttcc
     7021 cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg gtgttccgat
     7081 acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc gaggaatcgc
     7141 caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg cccaggttac
     7201 gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg agacgcgttt
     7261 tttgctgtgc agtatcactc tccgctttat ttccggcaaa ctccgcttcc caccgaccat
     7321 aaccagtcag ttgatcgtta atttgagttt cacctttaaa accaaaacgg atataactct
     7381 ggtcgccatc tttactggcg ttatcactca tataatgcat ggctttaact ttgccataga
     7441 catccagttt attaccgtct ttattatata tttctgcagc ctgtacagat gcagatgcca
     7501 caatgcccat caccactaat gccagagtgc tctttttcat tttcattcct gattttaatt
     7561 aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag gaaagttttt aacctgagat
     7621 tgttaaagat atattacaga ttaatgatat tctaaaaatg tggtaattta ttaaatctgt
     7681 aataaaagcg taaacaactg ccgctacgct tgctgatccc gcgcaacaaa acgccatgct
     7741 ttgctcgcag atggttggca accgacgaca gtcctgctaa aacgttcgtt tgatatcatt
     7801 tttcctaaaa ttgaatggca gagaatcatg agtgacagcc agacgctggt ggtaaaactc
     7861 ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat cgttgaactt
     7921 gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt gacgtcgggc
     7981 gcgatcgccg ccggacgtga gcacctgggt tacccggaac tgccagcgac tatcgcctcg
     8041 aaacaactgc tggcggcggt agggcagagt cgactgattc aactgtggga acagctgttt
     8101 tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat ggaagaccgt
     8161 gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgatcacaa tatcgttccg
     8221 gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aggtcggcga taacgataac
     8281 ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct gaccgatcaa
     8341 aaaggtttgt ataccgctga tccgcgcagc aatccgcagg cagaactgat taaagatgtt
     8401 tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg cctcggaact
     8461 ggcggcatga gtactaaatt gcaggccgct gacgtggctt gccgtgcggg tatcgacacc
     8521 attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg catttccgtc
     8581 ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg gattttcggt
     8641 gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat tctggaacgc
     8701 ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc gcgtggtgaa
     8761 gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc acggtgtcag tcgttacaac
     8821 agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc aatactggga
     8881 tatgaatacg gcccggttgc cgttcaccgt gatgacatga ttacccgtta aggagctggc
     8941 cgatgctgga acaaatgggc attgccgcga agcaagcctc gtataaatta gcgcaactct
     9001 ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa gcacaaagcg
     9061 aaatcatcct caacgctaac gcccaggatg ttgctgacgc gcgagccaat ggccttagcg
     9121 aagcgatgct tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt gccgacgatg
     9181 tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt aatcgatggc ggcgtactgg
     9241 acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc gtgatttatg
     9301 aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc ggtaatgcgg
     9361 tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc ggcaacggtg gcggtgattc
     9421 aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt gcaggcgatt gataatcctg
     9481 accgtgcgct ggtcagtgaa atgctgcgta tggataaata catcgacatg ctgatcccgc
     9541 gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg gtgatcacag
     9601 gtggtatagg cgtatgccat atttacgttg atgaaagcgc agagatcgct gaagcattaa
     9661 aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg taatacggtt gaaacgttgc
     9721 tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa atggcggaaa
     9781 gcggcgtgac attacacgca gatgcagctg cgctgacaca gttgcaggca ggccctgcga
     9841 aggtggtggc ggttaaagcc gaagagtatg acgatgagtt tctgtcatta gatttgaacg
     9901 tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat tcgtgaacac ggcacacaac
     9961 actccgatgc gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt aacgaagtgg
    10021 attcgtccgc tgtttacgtt aacgcctcta cgcgttttac cgacggcggc cagtttggtc
    10081 tgggtgcgga agtggcggta agcacacaaa aactccacgc gcgtggccca atggggctgg
    10141 aagcactgac cacttacaag tggatcggca ttggtgatta caccattcgt gcgtaaataa
    10201 aaccgggtga tgcaaaagta gccatttgat tcacaaggcc attgacgcat cgcccggtta
    10261 gttttaacct tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg gccgatatag
    10321 ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt aggttcgact cctattatcg
    10381 gcaccattta aatcaataag ttacctcgca tttaagtaaa ccacgttctc ctcttgtgcc
    10441 gtatttgtgc cattgcgact tataatcgca tcgattttgc ttgcgtgctc ggtgagatgc
    10501 ccggctgaaa ggtgggcgta tctttgaacc atttcgagag tttcccatcc tcccatctct
    10561 ttaagtgcaa gaagagagac accggactga accagccagc ttgcccaggt atgcctcaga
    10621 tcatggaagc ggaagttgct aatgcctgcc cgctttaacg ctcccttcca tgccttgttg
    10681 ctgtcggttc tcatcttcct taccgctgct gtttttgttc cgtcgcttcg gtaggcaggt
    10741 ttggtgtgga caaataccca tctcttatgg agcccctgct gttttcttaa tatctggcat
    10801 gcggtttcgt taagaggaac tccgatcgca ttgccagctt ttgtttcatc agggtgcatc
    10861 catgccattt tcttatccag atcgacctgt gaccactcaa ggtctgtaac gttggaacgg
    10921 cgaaggcctg tcgtgattgc aaacatgacc acagggaaga agtgaggagc aatttctgca
    10981 aacaggcgct tcgattcctc ctctgtaagc catctgatgc gtccattctt aacgcgtggt
    11041 gttgatattt tgggtgccct gtcaagccat ccccattcaa cagccatatt gagaatggcg
    11101 cgaagtattg ccagatgcct cgtcttcgtt cctttgcttg caagctttgg tttatactcc
    11161 ggcaccggat tgccaagtcg caaacacctg tcccggctca tctcccagtt caggcgatgg
    11221 cggcggtttt ccatcccgtc taccgcctcc attatttttt ctgttgttat gtcagagaga
    11281 atggcttctc tgaagtgcaa catccagaac gatataatgc tcttgtcatc atcaatggac
    11341 ttcttatccg atttctcacg cagccaccgt atgcaggctt ccttgaatag ctttttcggt
    11401 gattccccga gattttttac tctcca
//
LOCUS       DAJTYF010000120        11403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTYF010000120 DAJTYF010000000
VERSION     DAJTYF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.120"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..105)
                     /locus_tag="OO590_003353"
     CDS             complement(<1..105)
                     /locus_tag="OO590_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR6638606.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFC"
     gene            complement(222..806)
                     /gene="pth"
                     /locus_tag="OO590_003354"
     CDS             complement(222..806)
                     /gene="pth"
                     /locus_tag="OO590_003354"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6638607.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            1084..1362
                     /gene="ychH"
                     /locus_tag="OO590_003355"
     CDS             1084..1362
                     /gene="ychH"
                     /locus_tag="OO590_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6638608.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(1417..3096)
                     /gene="dauA"
                     /locus_tag="OO590_003356"
     CDS             complement(1417..3096)
                     /gene="dauA"
                     /locus_tag="OO590_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6638609.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(3221..4168)
                     /gene="prs"
                     /locus_tag="OO590_003357"
     CDS             complement(3221..4168)
                     /gene="prs"
                     /locus_tag="OO590_003357"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6638610.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(4319..5170)
                     /gene="ispE"
                     /locus_tag="OO590_003358"
     CDS             complement(4319..5170)
                     /gene="ispE"
                     /locus_tag="OO590_003358"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6638611.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(5170..5793)
                     /gene="lolB"
                     /locus_tag="OO590_003359"
     CDS             complement(5170..5793)
                     /gene="lolB"
                     /locus_tag="OO590_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6638612.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPTNMELTDGGQRIKLKMDNWIVK"
     gene            6007..7263
                     /gene="hemA"
                     /locus_tag="OO590_003360"
     CDS             6007..7263
                     /gene="hemA"
                     /locus_tag="OO590_003360"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6638613.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            7305..8387
                     /gene="prfA"
                     /locus_tag="OO590_003361"
     CDS             7305..8387
                     /gene="prfA"
                     /locus_tag="OO590_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6638614.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            8387..9220
                     /gene="prmC"
                     /locus_tag="OO590_003362"
     CDS             8387..9220
                     /gene="prmC"
                     /locus_tag="OO590_003362"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6638615.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            9217..9609
                     /locus_tag="OO590_003363"
     CDS             9217..9609
                     /locus_tag="OO590_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6638616.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            9613..10422
                     /locus_tag="OO590_003364"
     CDS             9613..10422
                     /locus_tag="OO590_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6638617.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            10458..11312
                     /gene="kdsA"
                     /locus_tag="OO590_003365"
     CDS             10458..11312
                     /gene="kdsA"
                     /locus_tag="OO590_003365"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6638618.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         2869 a         2885 c         2998 g         2651 t
ORIGIN      
        1 gcagaatgga aagttggccg cttcaatacc ggctttggtc agcgcgttga acagggtaga
       61 tttcccgacg ttgggcaaac cgacgatacc gcatttgaat cccatgattt aaatcacctt
      121 aatatcttaa taatcaacct gttattgcta acagattgca gaaatggaaa taactttgcc
      181 tattatacac ggcactcggc aaaaatgccg cagacaacga cttattgcgc tttaaaggcg
      241 tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc acatttcagt acaacgcgcc
      301 gcttcgtcga tggcttcatc aattaacttc tgttcactaa caggcggttt gcctaacaca
      361 aaaccgacaa ctttattttt atcgcccgga tgaccgattc cgatgcgtaa acggtgaaag
      421 ttagggttat tacccaattt actgatgatg tctttcagtc cattgtgacc accatggcca
      481 ccgcccaact taaatttggc gacgccagga ggcagatcca gttcgtcgtg ggccaccaga
      541 atttcgtccg gattaatgcg gaaaaaactg gccatcgccg caacggcttt gccgctgaga
      601 ttcataaatg tagtcgggac taacaggcgg acatcttcgc ctccaagggt gactcgcgaa
      661 gtataaccaa agaatttagc ctcttcgcgc agcggagcgc gcaaacgctc tgccagtaag
      721 tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt attcagcacc ggggttcgcc
      781 aggccgacaa tcaatttaat cgtcacgttt ttttgtcctg agtgtgtaca taactggcgc
      841 gtagtttact ggttgcggcc ccgcttgaca aaaaactgcg tatcaaatgc agataacgta
      901 ataattgcct gagtggacta ttagaaagtc aaggtgttca ggcgtttatt tgtaaagttt
      961 tgttgaaata agggttgtaa ttgtgattac gcccgcacat aacccacagg gtgttgtcta
     1021 tactttacac ataaggaaga ggggtattcc ctgttacaac ccagaaagtt ccggaggtga
     1081 catatgaaac gcaaaaacgc ttcgatactc ggtaacgtgc tcatggggtt gggtctggtg
     1141 gtaatggtgg tcggcgtggg gtattcaatc ctcaaccagt taccacagtt taatatgccc
     1201 cagtatttcg cacatggtgc agtgctaagt attttcgtcg gtgccattct ctggctggcg
     1261 ggtgcccgtg ttggcgggca tgaacaggtg tgcgaccgtt actggtgggt tcgtcactat
     1321 gacaaacgtt gccgccgtag cgataatcgc cgtcatagct aacaaataat gcagtttgct
     1381 gaccagtcag tttgcgctga ctggtcaatc tcgtacttat aaatccgcca tcgccgcgcg
     1441 acgattcggg aagaacgcga ggcgtcccgg gatcggttga atgcctgcgc gagccatagt
     1501 tcgcagtggc tggaattcca cgttgcacac gcgcagttca catccttcgg gcagacgctt
     1561 cacaaaacgc tggaacgcat caagaccacc agcatcaagt accggaacgg catcccactt
     1621 cagaatcaca atccgtttgc cttcaagacg tgactccagg tccgtgaata agccttcagc
     1681 agcagcaaaa aacagcgggc caataacgcg cagaaccagg acatcgtctg gaacatctac
     1741 gactaccggt gccaggcgag tcatacgtgc gatacgacgc ataaacagca gcgatgccag
     1801 cacgatcccc acgctgatgg caataaccat atcaaacagc acggtcagtg acatacacag
     1861 cagcatgacg atgatgtcat ctttcggcgc atgacgcagc aagtcgacca ctttgtgcgc
     1921 ttcactcatg ttccacgcca ccatcaacag cagggctgcc atggcggaaa gcggcagcca
     1981 ggagagcagt ggtgccagta ccagcagggc gagaataacc agaatagagt ggatcaccgc
     2041 cgagataggg gaagttgccc cggcacggac gttagcggca gaacgcgcga tggcagcagt
     2101 agcggtaata ccaccaaaga atggagcgat gatattcccc agcccctgac cgaccagttc
     2161 actattcgcc ttgtgcttag tcccggtcat accatccagt accacagcgc agagcagaga
     2221 ttcgatcgcc ccgagcattg ccattgagaa tgccgcaggc agcagggtgc gaatagaatc
     2281 ccaggttagc gtgaattctg aattaggcag atcccacggc agcaccagtt gcggcagcag
     2341 ttgcggaata ccgttaccct gagaaccatc ggccaggacg tagtggaatt gcgaaccgat
     2401 ggtagcaaca tgtccgccga gcaggttaac aatccccatc accgcgcaac cagccagcaa
     2461 ggccggaagg tgaccgggta aacgaatgcc cagacgcggc cagaaaacca gaatgccaag
     2521 cgtcacaatg ccaatggcag catcacccac attaatggtc ggcagcgcca taaataatgc
     2581 gccgactttt tgtagataat gttccgggac atgggccatt tgcagaccga gaaaatcttt
     2641 aatctgcatg gtaccgatgg tgatcccgat acccgaggtg aaacctaagg tgacggaaac
     2701 cggaatatac tcaatcagac gaccaaagcg tgccagcccc atcagaatca aaaagattcc
     2761 cgacaacaag gtcgcaacca gcagtcctgc cagtccaaac tgttgcgaaa cgggatagag
     2821 aattaccaca aatgccgcag tcggaccgga aacgctaaag cgtgacccac ccgttagagc
     2881 aatgacaatc cccgcaacag ctgcggtata taaaccgtac tggggtgcca caccactacc
     2941 aatagccaac gccatcgcca gcgggatagc aataatcccg acggttatcc cggcaatcag
     3001 gtcacgggta aaccgtgcgg cagtatattt ttctttccag caagcgtcga tcagagcgcg
     3061 gaaaggcatc acatgtgagg aaaatatttt gttcacaata atgtttcatc cgtgagcgca
     3121 tcatctgtca actaaatggc aggtgaagga ggcataggtc atacaaatgg atattacaga
     3181 caaaaaaacc cgccgcagcg ggtctttgag ccgggttcga ttagtgttcg aacatggcag
     3241 agatcgattc ttcgttgctg atacgacgaa tcgcttcggc cagcatacct gacagggtca
     3301 gagtacgcac gttcggcagt gatttgattt catcgctcag cggaatggta tcgcagacaa
     3361 cgacttcatc aattacagag ttacgcaggt tgttcgccgc gttgccagag aagatcgggt
     3421 gagtcgcgta cgcaaataca cgtttagcac cacgttcttt cagcgcttca gcagctttac
     3481 acagcgtacc gccagtgtcg atcatatcat cgaccagtac gcagtcacga cctgcaacgt
     3541 caccgatgat atgcatcacc tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg
     3601 ccatatcggt atcgttcagc agcttagcga tagcgcgggc acgcacaacg ccgccgatgt
     3661 ccggagaaac cacaattggg ttatccagat tcagctgcag catgtcttcc agcaggatcg
     3721 ggctaccaaa tacgttatca accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca
     3781 gatccactgt cagcacacgg tcaacaccga cgctggagag gaagtctgca accactttcg
     3841 cagtgattgg tacacgagcg gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg
     3901 ggataacagc ggtgatacga cctgcggaag cacgacgcag ggcatcaacc ataacgacta
     3961 attccatcag gttgtcgtta gtaggggcac aagtggactg gatgatgaaa atatcaccac
     4021 cgcgtacatt ttcgttaatt tgtacgctga cttcgccgtc gctaaagcga cctacagcgg
     4081 cgtcgccgag tgaagtgtac aggcggttgg caatacgttg tgctagtacc ggggtggcgt
     4141 taccagcaaa aagcttcata tcaggcacga gaagaacctc aggcatgcgt ccattggtgg
     4201 aaagaatctg ccgaaaactg tgcgggccag gcatgatcct ttccaggcgg tgtattaaag
     4261 agcgcgatgc aacgtctgga acaaggtgac gttgtcaccg aaactcagct tgcccggctt
     4321 aaagcatggc tctgtgcaat ggggaaagat taacgccttt cgccacaaag ccattgagcc
     4381 attccggggc ttgctctagc acctggcggg cttcagactc tgtatcaaat tcagcaaaga
     4441 cacaggcccc tgtcccagtc aggcgcgacg gggcgtattt taacagccag gaaagcaccg
     4501 catcaacctc gcgaaaacgt tttcttgcga taacctcgca atcattgctg aattcacatt
     4561 ttagcaacgt ttctattgac ctttttggcg tattgcgcgg gagttcagga tctttaaaaa
     4621 tcaccggagt cggaatactt acaccagggt gcgccaccag ataccacttc tctggcggat
     4681 ccaccggcgt tagtatttca ccaacgcctt cggcaaacgc ggcatgcccc cgaacaaaga
     4741 caggaacatc tgcgcccagc gtcagcccca tttccgccag ctcatccatg cttagcccgc
     4801 attgccagag atgatttaat gccaccagga ccgtcgcggc attggatgaa ccaccgccga
     4861 gaccgccgcc catcggcaaa cgcttgtcaa tgctgatatt cgcaccgctt cccgtcggaa
     4921 gacgcccgct gtctgccgca gttttcatca acaaccgcgc tgcgcgaacg atcaggttat
     4981 cttcatgttc cacgccttca acgggcgtta acagacgaat atccccatcg tcacgaagct
     5041 caatgctgat ggtgtcgccg taatcaagaa actgaaacag cgtttgcagc gtgtggtaac
     5101 catccgcacg ctgaccggta atgtataaaa acagattaag ttttgccgga gagggccact
     5161 gtgtccgcat tatttcacta tccagttatc catttttaac ttgatgcgtt gaccaccgtc
     5221 ggtgagttcc atattggttg gcatcgcagg ttgcgttttg gtgtcataac caccataaac
     5281 aaccttccag tttttgccat tctggctgta ggtaatttcg ctcaggcggt actggtcgtc
     5341 cagtttgtag tcggttgcat cacccggtaa acctaaaatc cactggcgca agctgttgag
     5401 cggaattggc attccggtca atttgccaat catctcttcg gcgtcatcgg cggtataacg
     5461 ctgaccttta ttgtcgacta actgcacgtt acccggttga gcattcagct ccagttccgt
     5521 gctgcccaat gggttagtga gcagcagacg gtagcgatcc tggccggttt gctgccagaa
     5581 aaagcgggcg tacacttttt gttggtcaga aatataagcg aacgcgccgc gagtctgata
     5641 ctgattaaga ttgcgcacgt cttgctgatg ctgacgccat tgtggcgaat ccgggctttt
     5701 gccaggacct ttgggcgtgg taacggaaca ggcagtgagc acaagagcag ccagcggtag
     5761 caggcggata agacgaaaat cgggcagggg catagtgatg acaagtcctt gagatacgtt
     5821 gcagttataa cccttaatgc tagcgttacc gtccgctatc gtctatgttc aagttgtctt
     5881 aattgccaga atctaacggc tttcggcaat tactccaaaa gggggcgctc tcttttattg
     5941 atcttacgca tcctgtatga tgcaagcaga ctaaccctat caacgttggt attatttccc
     6001 gcagacatga cccttttagc actcggtatc aaccataaaa cggcacctgt atcgctgcga
     6061 gaacgtgtat cgttttcgcc ggataagctc gatcaggcgc ttgacagcct gcttgcgcag
     6121 ccgatggtgc agggcggcgt ggtgctgtcg acgtgcaacc gcacggaact ttatcttagc
     6181 gttgaagagc aggataacct gcaagaggcg ttaatccgct ggctttgcga ttatcacaat
     6241 cttaatgaag aagatctgcg taaaaacctc tactggcatc aggataacga cgcggttagc
     6301 catttaatgc gtgttgccag cggcctggat tcactggttc ttggggagcc gcagatcctc
     6361 ggtcaggtta aaaaagcgtt tgccgattcg caaaaaggtc atatgaaggc cagcgaactg
     6421 gaacgcatgt tccagaaatc tttctctgta gcgaaacgcg ttcgcactga aacagatatc
     6481 ggtgccagtg ctgtgtctgt cgcttttgcg gcttgtacgc tggcgcggca gatctttgaa
     6541 tcgctctcta cggtcacagt gttgctggta ggcgcgggcg aaaccatcga gctggtagcg
     6601 cgtcatctgc gcgaacataa agtacagaag atgattatcg ccaaccgcac tcgcgaacgt
     6661 gcccaaattc tggcagatga agttggcgcg gaagtgattg ccctgagtga gatcgacgaa
     6721 cgtctgcgcg aagccgatat catcatcagt tccaccgcca gcccgttacc gattatcggg
     6781 aaaggcatgg tggagcgcgc attaaaaagt cgtcgtaacc aaccaatgct gttggtggat
     6841 attgccgttc cgcgcgatgt tgagccggaa gttggcaaac tggcgaatgc ttatctttat
     6901 agtgtggacg atctgcaaag catcatttcg cacaacctgg cgcagcgtaa agccgcagcg
     6961 gttgaggcgg aaactattgt cgctcaggaa accagcgaat ttatggcgtg gctgcgagca
     7021 caaagcgcca gcgaaaccat tcgcgagtat cgcagccagg cagagcacgt tcgcgatgag
     7081 ttaaccgcca aagcgttagc ggcccttgag cagggcggcg acgcgcaagc cattatgcag
     7141 gatctggcat ggaaactgac taaccgcttg atccatgcgc caacgaaatc acttcaacag
     7201 gccgcccgtg acggggataa cgaacgcctg aatattctgc gcgacagcct cgggctggag
     7261 tagcagtaca tcattttctt tttttacagg gtgcatttac gcctatgaag ccttctatcg
     7321 ttgccaaact ggaagccctg catgaacgcc atgaagaagt tcaggcgttg ctgggtgacg
     7381 cgcaaactat cgccgaccag gaacgttttc gcgcattatc acgcgaatat gcgcagttaa
     7441 gtgatgtttc gcgctgtttt accgactggc aacaggttca ggaagatatc gaaaccgcac
     7501 agatgatgct cgacgatcct gaaatgcgtg agatggcgca ggatgaactg cgcgaagcta
     7561 aagaaaaaag cgagcaactg gaacagcaat tacaggttct attactacca aaagatcctg
     7621 atgacgaacg taacgccttc ctcgaagtcc gtgccggaac cggcggcgac gaagcagcgc
     7681 tgtttgcagg cgatctgttc cgtatgtaca gccgttacgc cgaagcccgc cgctggcggg
     7741 tagaaatcat gagcgccagc gagggtgaac atggtggtta taaagagatc atcgccaaaa
     7801 ttagcggtga tggtgtgtat ggtcgtctga aatttgaatc cggcggtcat cgcgtgcaac
     7861 gtgttcctgc tacggaatcg cagggtcgta ttcatacttc tgcttgtacc gttgcggtaa
     7921 tgccagaact gcctgacgca gaactgccgg acatcaaccc agcagattta cgcattgata
     7981 ctttccgctc gtcaggggcg ggtggtcagc acgttaacac caccgattcg gcaattcgta
     8041 ttactcactt gccgaccggg attgttgttg aatgtcagga cgaacgttca caacataaaa
     8101 acaaagcgaa ggcgctttca gtgctcggtg ctcgcatcca cgctgctgaa atggcaaaac
     8161 gccaacaggc cgaagcgtct acccgtcgta acctgctggg gagtggcgat cgcagcgacc
     8221 gtaaccgtac ttacaatttc ccgcaggggc gcgttaccga tcaccgcatc aacctgacgc
     8281 tctaccgcct ggatgaagtg atggaaggta agctggatat gctgattgaa ccgattatcc
     8341 aggaacatca ggccgaccaa ctggcggcgt tgtccgagca ggaataatgg aatatcaaca
     8401 ctggttacgt gaagcaataa gccaacttca ggcgagcgaa agcccgcggc gtgatgctga
     8461 aatcctgctg gagcatgtta ccggcagagg gcgtactttt attctcgcct ttggtgaaac
     8521 gcagctgact gacgaacaat gtcagcaact tgatgcgcta ctgacacgtc gtcgcgatgg
     8581 tgaacccatt gctcatttaa ccggggtgcg agaattctgg tcgttgccgt tatttgtttc
     8641 tccagcgacc ttaattccgc gcccggatac ggagtgtctg gtggagcagg cactggcgcg
     8701 gttgcctgaa caaccttgcc gtattctcga tctcgggacg gggaccgggg cgattgcgct
     8761 tgcgctggct agcgagcgcc cggactgcga aattatcgct gtagatcgta tgcctgatgc
     8821 tgtctccctg gcacaacgta atgcccagca tctggcgatc aaaaatatcc acattctgca
     8881 aagcgactgg tttagcgcgc tagccgggca gcagtttgcg atgattgtca gcaatccgcc
     8941 gtatattgac gagcaggacc cacatcttca acaaggcgat gtccgctttg agccgctcac
     9001 tgcgctggtt gcggcagaca gtggaatggc agacatcgtg catatcatcg aacagtcgcg
     9061 taacgcgctg gtatccggcg gctttctgct tctggaacat ggctggcagc agggcgaagc
     9121 ggtgcgacaa gcatttatcc tcgcggggta tcatgacgtc gaaacctgcc gtgactatgg
     9181 tgataacgag cgcgtaacgc tcggccgcta ttatcaatga caagtttttc tacactgctt
     9241 agtgttcatc ttattagtat cgcgctttct gttgggctat taaccttacg tttctggcta
     9301 cgttatcaga agcatccaca ggcatttgct cgctggacgc gcattgtgcc gccggttgtc
     9361 gatacggtgt tattgttaag cggcattgcg ttgatggcta aagcgcacat cctgccattt
     9421 tccgggcagg cacagtggct gactgaaaag ctgtttggag ttatcattta tatcgttttg
     9481 ggttttattg cactcgatta tcgtcgtatg cacagtcagc aggcgcgcat tattgccttc
     9541 ccgctggcgt tggtggtgct gtacatcatc attaaactcg ccaccacaaa agtaccgtta
     9601 ctggggtaag ttatgagatc gttagctgat ttcgaattta ataaagcgcc attgtgcgaa
     9661 ggcatgatcc tggcttgcga agcaatccgc cgcgattttc cctcgcaaga tgtttacgac
     9721 gaactggagc gtctcgttag tctggcgaag gaagaaatca gccagcttct gcctttagaa
     9781 gagcagctgg aaaaactgat cgcgctgttt tacggcgact ggggatttaa agcctcacgc
     9841 ggcgtttatc gtctttccga tgcattatgg ctggaccagg tgttaaagaa tcgacagggc
     9901 agtgcggtat cattaggtgc ggttttatta tgggtcgcga atcgtctcga tttgccgctg
     9961 ctgccggtga ttttccctac gcagctgata ttgcgcattg aatgtccgga tggcgaaatt
    10021 tggctgatta atccttttaa cggtgaatcg ttaagcgaac atatgctgga cgtatggtta
    10081 aagggaaata tcagcccgtc ggcggaactg ttttatgaag accttgatga agctgataac
    10141 attgaggtaa tccgcaaatt gctggataca ctcaaagcct cgttgatgga agaaaatcag
    10201 atggagctgg cgttacgcac cagcgaagct ttattacaat tcaaccctga agatccctat
    10261 gaaattcgcg atcgcgggtt gatttatgcg caactggatt gcgaacacgt tgcgttgaac
    10321 gatttaagtt atttcgttga acagtgtccg gaagacccga tcagcgaaat gatccgtgcg
    10381 caaataaata acatcgcgca taaacatatt gtgctgcatt aattaatcga cattttactc
    10441 aagattaagg cgatcctatg aaacaaaaag tggttagcat tggcgacatc aacgtagcaa
    10501 atgacctgcc gttcgtactg tttggcggta tgaacgtgct ggaatctcgc gatctggcga
    10561 tgcgcatttg cgagcactac gtaactgtga cccagaaact gggtatccct tacgtgttca
    10621 aagcctcttt tgacaaagcc aaccgctcct ccatccactc ttatcgtgga ccgggcctgg
    10681 aagaagggat gaaaatcttc caggagttga agcagacttt tggcgtgaaa attatcaccg
    10741 acgttcacga accaagtcag gcacagcccg ttgctgatgt cgtggatgtg attcagttgc
    10801 cggcgtttct tgctcgccag actgacctgg ttgaagccat ggcgaaaacc ggtgcggtaa
    10861 ttaacgtcaa gaaaccacag tttgtcagcc cggggcagat gggtaatatc gttgataaat
    10921 tcaaagaagg cggcaacgaa aaagtgattc tttgcgatcg tggtgctaac ttcggctatg
    10981 acaacctggt tgtcgatatg ctgggcttca gcattatgaa gaaagtgtct ggtaactcgc
    11041 cggtgatttt cgacgtgacc cacgcactgc aatgccgcga tccgtttggc gcagcttccg
    11101 gtggtcgtcg tgctcaggtg gctgagctgg cacgagccgg tatggcggta ggtctggcgg
    11161 ggctgtttat tgaagcgcat ccggatccgg aacatgcgaa atgtgatggt ccatccgcgc
    11221 tgccgctggc taaactggaa ccgttcctca agcagatgaa agcgattgat gatctggtga
    11281 aaggtttcga agaactggat accagcaagt aatctttttt gcttgaaaaa taaagtctta
    11341 gcgttctgcg ttaagacttt tttcatgggt gccagataca aaaaaggccg caggctgtta
    11401 ccc
//
LOCUS       DAJTYF010000121        11323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTYF010000121 DAJTYF010000000
VERSION     DAJTYF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.121"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..212
                     /gene="yfdR"
                     /locus_tag="OO590_003366"
     CDS             <1..212
                     /gene="yfdR"
                     /locus_tag="OO590_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6638619.1"
                     /translation="VMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVI
                     PLAPGHAYGMFMERFNELSELRKCA"
     gene            203..565
                     /locus_tag="OO590_003367"
     CDS             203..565
                     /locus_tag="OO590_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638620.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            565..870
                     /locus_tag="OO590_003368"
     CDS             565..870
                     /locus_tag="OO590_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638621.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGNNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            1097..2260
                     /locus_tag="OO590_003369"
     CDS             1097..2260
                     /locus_tag="OO590_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6638622.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVVTKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKESTNDV"
     gene            2610..2972
                     /locus_tag="OO590_003370"
     CDS             2610..2972
                     /locus_tag="OO590_003370"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HCR6638623.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            2969..3886
                     /locus_tag="OO590_003371"
     CDS             2969..3886
                     /locus_tag="OO590_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6638624.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            3899..5164
                     /locus_tag="OO590_003372"
     CDS             3899..5164
                     /locus_tag="OO590_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR6638625.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(5231..5854)
                     /locus_tag="OO590_003373"
     CDS             complement(5231..5854)
                     /locus_tag="OO590_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6638626.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(6605..6943)
                     /locus_tag="OO590_003374"
                     /pseudo
     CDS             complement(6605..6943)
                     /locus_tag="OO590_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            7065..7325
                     /locus_tag="OO590_003375"
     CDS             7065..7325
                     /locus_tag="OO590_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCR6638627.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(7343..10078)
                     /locus_tag="OO590_003376"
                     /pseudo
     CDS             complement(7343..10078)
                     /locus_tag="OO590_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(10079..>11323)
                     /locus_tag="OO590_003377"
     CDS             complement(10079..>11323)
                     /locus_tag="OO590_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6638628.1"
                     /translation="NTLQLIRQNNDIERSGANNVGLTALQGLSGIAGVFQQEKQAQRQ
                     KEFQQAYANAYASGDRGALRQLATQYPDQIESVRKGMGFIDEDQRNSIGTLAAGARLA
                     SSSPEAMQSWLQNNAGELARVGVNPQDVAQMYQQNPRQFGEFVDHLGMNSLGPEKYFD
                     LQDKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRINLDAETNRINNENK
                     RLDRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGINTLTTSMLTLNDIV
                     SSPSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQAMRGMGALSDAEGKK
                     LDQAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNGIKRVEAPQASIDYL
                     KQHPELSIDFINRYGYLPSLGQ"
BASE COUNT         3066 a         2577 c         2508 g         3172 t
ORIGIN      
        1 cagttatgag tacgcccgtg aaatatgccg atctcatcat gctggcaacc gaacgccgcg
       61 atctcgggct tgatgatggc tctttctggc ctgtactgga aggtatcccg gcaacagaga
      121 tgttcaacgt gattccactg gcaccgggcc atgcctacgg gatgtttatg gaacgcttta
      181 acgagttatc ggagttacgc aaatgcgcat gaatgttttc gaaatggaag ggtttcttcg
      241 tgggagatgt gtaccgcgag atctgaaagt aaatgaaaca gatgctgaat acctggtgcg
      301 taaattcgat gcgcttgaag ctaaatgtgc agcacaggaa aacaaagtaa taccagtgtc
      361 aactgaactg ccaccagcaa atgaaagtgt tttgttattc gatgctaacg gagaaggctg
      421 gctaattggt tggcgttctc tctggtacac ctggggacaa aaagaaaccg gagaatggca
      481 gtggacattt caggtcgggg accttgaaaa cgtcaatatc actcactggg cagtaatgcc
      541 aaaagcaccg gaggctggag cataatgacc acttttaccg acaaagaact gattaaagaa
      601 attaaagagc gtatcagcag ccttgacgtg cgagacgata ttgagcgccg tgcttatgaa
      661 atcgcactcc tatctctgga agtagaacca gatgaacgcg aagcttatga attattcatg
      721 gaaaagcgtt tcggtgactt agtagatcgt cggagagcaa aaaacggcaa taacgaatac
      781 atggcatggg atatgactct cggttggatc gtctggcagc aacgagctgg tatccatttt
      841 tcaacaatgt cacagcaaga ggtgaaataa tggagccata cagcctcaca ctcgatgagg
      901 cctgtcattt tctcaagata tccagaccga ctgccattaa ctggatacgc acagggcgtc
      961 ttcaggcaac acgcaaagat cccactaaga ataaatctcc ttacctcaca acacgacaag
     1021 cctgcattgc ggctcttcag tctccgctgc atactgtcca ggtgagcgcg ggtgatggca
     1081 taacagagga aagaaaatgt cactcttccg cagaggtgaa atatggtacg ccagtttcac
     1141 attgccgaac ggtaaaagat ttaaacagtc tcttggaaca aaggacaaaa ggcaggcgac
     1201 agaactccat gacaagctaa aggctgaagc atggcgggtc agcaaacttg gtgaaatacc
     1261 tgatataacg ttcgaggaag cgtgtgtcag gtggcttgaa gagaaagcac ataaaaaatc
     1321 actggacgat gacaaaagcc ggatcggatt ctggcttcaa catttcgcag gaatgcaact
     1381 aagagacatt actgaatcaa aaatttattc agcaatgcag aaaatgacga accggcgtca
     1441 tgaggaaaac tggaaactca gggcagaagc atgcagaaaa aaagggaaac ctgttccaga
     1501 atacacgcca aaaccagcgt ccgttgtaac gaaggctacg catctttcat ttataaaggc
     1561 cctactaaga gccgcagagc gtgaatggaa aatgctggat aaggcaccaa ttattaaagt
     1621 gcctcaacca aagaataaac ggatccgctg gctggagccc catgaagcac aaaggctgat
     1681 tgatgaatgt ccggagccat taaagtctgt tgttgaattt gcactggcaa caggcttaag
     1741 acgctcgaac atcatcaacc ttgaatggca acaaatagat atgcagcgcc gggtggcatg
     1801 gataaacccg gaagagagta aatcaaaccg cgcaatcggc gttgcgctga atgatactgc
     1861 atgtcgcgtt ttgaaaaaac aaatcggaaa tcatcaccgt tgggtatttg tgtacaagga
     1921 aagctgtacc aaaccagacg gaacgaaagc gccaacagta aggaagatgc ggtatgacgc
     1981 aaacacagcc tggaaagcgg cgctgagacg ggctggtatt gatgatttca gatttcacga
     2041 cttgagacac acctgggcaa gttggctggt tcaagccgga gtcccgttgt cagtgttaca
     2101 ggaaatggga ggctgggagt ctatcgaaat ggttcgtcga tatgctcacc ttgcacctaa
     2161 tcaccttacc gaacacgcac ggcaaataga ctcgatcctg aacccatcgg tcccaaattt
     2221 gtcccagtca aaaaataagg aaagtactaa tgatgtgtaa cttattgatt taaatggtgc
     2281 cgataatagg agtcgaacct acgaccttcg cattacgaat ctgtagcacc aatcataact
     2341 atctgtttta gcaagcatta accgcattca ctaagatata gttgatggca caaacagaaa
     2401 gttgatgaat gatgttgcca tatatatgtc acaaatacgg cacaacgatc ttcaaacata
     2461 tagccacaca ttcacagaag agcacaaagc cttgcaatcc agtgcaaaga tttgtgtgcc
     2521 agagtttttc tcatcaacta ccgcaagtat cgatcgattg agacttggat gatagacttc
     2581 atgcctttga ttattagctg atagaagaaa tgttaaagct atttgcaaag tacacatcta
     2641 taggcgtgct gaacacactt atacactggg tggtttttgg tgtatgtatc tatgccgcgc
     2701 ataccaatca ggctatggca aacttcgcag gtttcgttgt ggctgtgagt tttagcttct
     2761 ttgcgaatgc aaaattcaca ttcaaagcat caactacaac gatgcgctac atgttatatg
     2821 tcgggttcat gggaacactg agtgctactg ttggatgggt tgctgataga tgttcacttc
     2881 ctccaattgt cactcttgtt accttctccg ccatcagctt ggtgtgcggt ttcgtctatt
     2941 caaagttcat tgtctttagg gatgcgaaat gaagatatct cttgtcgttc ctgtcttcaa
     3001 tgaagaagaa gcgataccaa ttttttataa aacggtacgt gaattcgaag agttgaagcc
     3061 atatgaagta gaaattgttt tcataaatga cggaagcaaa gacgctacgg agtcaatcat
     3121 taacgctctg gctgtttctg atccgctagt tgttccgctg tcatttacac gcaactttgg
     3181 taaagaacca gcattgtttg cagggttaga ccatacaacc ggcgatgccg tgattccaat
     3241 tgatgttgac ctgcaagacc cgattgaggt tattcctcat cttattgaaa agtggcaggc
     3301 aggtgctgac atggtgcttg ctaaacgttc agaccgctca actgatggac gactgaaacg
     3361 taagacagct gagtggttct ataaattaca caacaaaata agcaccccaa aaatagagga
     3421 aaatgtcgga gattttcgac tcatgtctcg tgaggttgtg gagaacatta aactattgcc
     3481 tgagcgcaat cttttcatga aaggcatact gagctgggtt ggtggtcaga cagatgtcgt
     3541 tgaatatgta cgcacagagc gtgttgctgg catctcaaaa tttaatggct ggaaattatg
     3601 gaatctggca ctggaaggta tcacaagttt ttcaaccttt cctcttcgcg tatggactta
     3661 tataggcttg tttgttgcaa gcatttcatt tttatatggt gcatggatga ttatagacac
     3721 cattgtcttt ggtaacccag tacgcgggta tccctccatg cttgtatcaa tacttttcct
     3781 gggtggagtg caactgatcg ggattggtgt tctcggtgaa tatataggta gaatttattt
     3841 agaaacaaaa tcaagaccac ggtacttaat taaaagcaga aaataacagg atgatcttat
     3901 gaagatattc ataatgcgca attggcacaa gatatcaatt tttatacttg catttacttt
     3961 gatttggctt aggcgcattg acattttgac aaatgcccag ttttgggctg aggatgcagt
     4021 tttttggtat aaagatgcat acgaacaagg ttttttcagt tcactcataa ccccaagaaa
     4081 tggttatttt caaacagtat caacactcat tgttggtgcg acgacattta ttaatccaat
     4141 ttacgcacca ttattatcaa acttttttgg aataataatt agagctataa ttatatggtt
     4201 cttatttacc gatagattta aattcctaag caccacatca aaaatcttca tctctgtata
     4261 tttaatttgc atgcccgggc ttgatgaagt ccaagccaat ataacaaatg ctcactggta
     4321 tttatcattg tatgttgcaa tgataataat atctgaagaa tccaagtcaa aattatggaa
     4381 agcacatgat ttatttttta tagtattatc tggattgagc ggtccattta ttatattcat
     4441 aattgcatca tctgttttta aatatttaca cttatcaaaa ggagtaataa gcataagagg
     4501 tttatactta ttttatactc gactgccata tttggcaatg atcttgtgtg ggctgataca
     4561 ggcaacatca ataattctta catttaatgg aacaagatca catgctccat taggattcag
     4621 ttttgatgtt atgtcatcta taatatcatc taatgtattt cttttttcat ttgtcccttg
     4681 gaatatcgct aatgctggat gggataatca ccttttatcg tacacactat caactttagt
     4741 aattacatta tgtgttttca tttatattaa aggaaattgg caaatgaaag tgtttgccac
     4801 ccttccaata cttattgtcg cattttctat ggcaaaacct caactggcag attctgttcc
     4861 tcaactgcca acacttgcaa ccggaaacgg gtcaaggtat tttgtaaata ttcatattgc
     4921 aattttttca ttaatatgcg tttgtttgtt tcaatgcata aagaataaaa ccttaaaaat
     4981 attctttaaa atctatgttt cagtcatcct tattgcaatg ataaaattaa acttttttat
     5041 aacccctctt cctgacatga actggtctca aggagcagaa ttaataaata aagcaaaaca
     5101 tggagaagcc gtctcattta cagtgctccc tccatggtta actcttgatt taataaaaaa
     5161 ataaaacttc acattaataa ataacaatga atccgcctcc ctacgtattc ccgtagggag
     5221 gctgtcgtgt ttatagtagc ctggtaatgc taaatgcaaa cgaactggag attcctgctc
     5281 cgctaagcgt attgcgcagg gtcaccctaa atgtatctac accaatataa gtaacatcac
     5341 aactaactga atcaggcgca gatataggtg ttatctgaac cacataacca ttagtagctg
     5401 ctgcgagcgg atgagaaaac gttacatcat acacaccagt agcatgcgcg ttaacggatg
     5461 aaacacccgg cgcaatgtct acactaccat tggcgttaat tctccctgag cgcgtaactg
     5521 actctgcaga aaccgatgtg gccttactgg ttatttccat aaaggtgcaa ccaatatcga
     5581 tcaattcgca attgttccct gttatccagt atggtcgatc tgcacttggc tgataatccc
     5641 ctgcgctctg gaacgttgtg ccattgagta agactataac ttttccccca cctgagcttg
     5701 ttacttgtat attcgtgtgc gtgtagcgtt cactgctcac gcgatgaaaa tttccaccgt
     5761 ttataactac cgttacaggt cgcgtcccag tattatctat agcaaggtct gcccccccgg
     5821 cattagcctc gaagtatggg ttgtttaaca ccaaagcaca cgaaccgtta aggccattta
     5881 tggctaattg cgcaccacct gtaccggaat ccccctgagt accatttcct tcacaagtca
     5941 ggctgttgat agtagttcct gccccccatc tatcaccaag tataccccat cgtgtattct
     6001 ggcttgcagt taatcggtta actgtaatgg cattaagctc tgacccttca ctggagttaa
     6061 gtgcgcgcca gccaatacgg ttaccctgta agtacacatt atctaactct gacgttaaca
     6121 ccgagtctgt acgcaaccct tcgccggtag tgtgcccata agacacaatg ttctctaatt
     6181 tagtgtatgc tttattggtt acgagaagac cgtaagacga tcccgaacct ttaagtgtaa
     6241 tgtcccccag ataatcatag gcgtgtacac gttgggttgc cccgactgta tcaccgagca
     6301 tctttaagtt gaaatccgag gcatggcagt taaatatcgt ttctgccata ttttctccgg
     6361 aataatgagc acggactcct gtcgaaccaa taaatttagc gtcatagttg ccgtaatcaa
     6421 gaacgagtgg tgacgcgata tcgtacaccc cttgctttat ttttaatgtt ccaccgaaag
     6481 gccacgatgt aagcgggtct tgatatattt cattttttat tgcaaatagc tcctcttgga
     6541 gatttttgcc gctattagtg ccaatgtgag cagctccttc atctgattga agtaactgct
     6601 ttaactgatc aggatcgtac ttcagcacat tctgaaaata gaactgctgc gaaccatacg
     6661 catcatatac agccattgaa tggccttgct cagttacaaa tttggcaatc tgcccgttgt
     6721 acacaggata tccggcagca ttgataacaa ttggctgaga aacagggacg tgagatccgt
     6781 cttcattctc tacataaacc tgaatctggt tttcaggatt taccgggtca gtgtcaattt
     6841 ttccgatata aattttgcca ttggctacgg ctttaaaaga acgcgccata gtgaagagtt
     6901 gcgaaggcat gctgaccaca acatttgcgg cgatatctga catttcattg ctccagacga
     6961 atgatatgat gcaatcatga tgtgattgca taccgaaatg gcactattga atatttatcc
     7021 agtaggttac gatgccattc cacccaactg gtgaggcatc aaggatgtac agcaaatacg
     7081 acgaggcaca gtttcacttg agacttccgc atgaactcca cgcgaaaatt aaacagtgtg
     7141 cgaagatgaa taacagttcg ctgaactcag agataattgc agcgattgaa gaatcattgg
     7201 ctaaacaaag ctctgcatca gtttacattg acgatgcaga gcgtatggca gaacaacaat
     7261 ctgatatggt taagaaaatt gtctttgata cgctcaagga gctatataaa aaagacagca
     7321 actaaccatc cgtggctttc cgttacttct ggttatcatc actgttcatc cagtacatga
     7381 tgcctagcct tgatatggtt ttcttatctg attctggaag tgagttgtaa aacgagcgcc
     7441 aagcagaaga gcgtcttaca gttgtatcaa cgatgctttc tggtgcttgt tttactgcca
     7501 gccttgctgc tttctggaat tctggtgagc taattagctt ttctgcagat tcaattgatt
     7561 cagcgccgcc catcgccctg attttgctgg cgacttttgc cccaatttct gacccagcaa
     7621 cagcacctac tgcactgaac ggtcctgaca cagttgaacc aaccgcagtg ccaatgcgtt
     7681 gggcatggtt agcaacaaat tcatttgccg cagtgacgcg attaaaccgt ttgacgaact
     7741 cgtttagtct tcctgtagtt atctcgtaag attttgcatc tttgatagcc tttgcgacct
     7801 tatatacatc gttcagacct gacatagtct cttttggtaa atgtctggca agattgcgca
     7861 tctgcccgtt tgctaacatg ttttgatacc agtcagcaaa ccctgcagga ttaaaatcag
     7921 cgccacgttt tccgttcgaa agcatatccc gaagacctgt tcccagtagc tcggttctta
     7981 gcttcctgga cggcgtgttt tgcatcaatt cacgaaatcc tttggcatcg cctttcgaca
     8041 ttgcctgtag tgctgtagtt gctttgcgag aaacatcacc gttcagcgtt ctgccagtta
     8101 gattaatcat ttgctcttcc atgctttttc gcatctgaac aagcctctga gcaacttcaa
     8161 aatcacgtaa tgcatttgtc tctgacagcg ccgccttttg atcatcggcg agcgaaccgt
     8221 aaagcctcga taaagcagcg cgatcagcat ctttatatgg tccagaattc ttatgaagtg
     8281 catcaccaac aagtcgtcgt tgcttattga gatttgcata agttaatgcg ccgttcttgc
     8341 ctggattaac tgcccgaaag actctctttt caatagggtc aaggtttttc atcccgccaa
     8401 gatcttctgc cacctgtttg agcattgacc ttgttgatgg cgcatcaatt tttgcacctg
     8461 caggcatcgc cttttcaaca ttgcgataaa gctggtcgct tcgtttcatc agcgcagaca
     8521 ttcttccatt gaccgtatca ataaacttgt cgctcatacc aagagcatct tttgcaccgg
     8581 aaacgcgatc aattatctca cccgcgccct gtgctagctg cctgattgct tcattttcct
     8641 gcacctgtag agcagaaccc gcacgggact tgattgcttg ctcaactgcc ttgtattgct
     8701 cgttcccgga aaagtgtgaa gggagtaatg aatcaacatt aagcctttca gcagactcca
     8761 gaacttcagc ctgaggattg atatctagtt catcaagcga tgaagcgaga tttggccttt
     8821 tttgtgctgc tactgtgcga acgacttcct ctgggttctt cgtcgcaggc gtcattggta
     8881 caccttgtgc tactcttgcc tctgcggcat gaaccgcagg atttgttgct gcagcggcat
     8941 caccggagaa tggtgatgtt tctgttggcg cagtggtcgc tctctgtgca gatgttttat
     9001 tgccactaat tatatttgat gcaccacgaa caccacgggt aacaccattt atcaggccat
     9061 gcgtagcaat gctaagtccg gcattaatag cggcattttc ggcaaaatca ccccgttgat
     9121 tcgccgcgtc agcgagagaa ccaatgacca tgtttccagc tacacctgct ccaggaacaa
     9181 gataaccgcc tattgactct ccagcttgcg cgtaggggtc tgtcggtctg tctactggac
     9241 gataaacatc atccaatact tttggccccc caagcccctg actgattgca ttaatcagac
     9301 ttgcgccacc ctgcaatacg tcaaatggta tgtttaccag accacgacca gcctgttctg
     9361 caatttaccc tgcactttga ccaccagtga gccaatcgcc agcttgttgc atcaatgatg
     9421 gttcttcccg tgttggtgca ttattggcct gattaactgt ttgttgctga acaggtttcg
     9481 gtgcgacaga acttacgggt tgaggtggct gctgactggc tgcctgctgc tcaatctgag
     9541 caaatggatt atttgggtct gactgcacac ctgatgccga tacttgttcg gatgactgta
     9601 cttccagttg tgcaaacggg ttgttagggt cttgctgagg atgtaccttt gcagaggtgg
     9661 cgcgctgttc gactgttgag tctgtcaccg ggtcaccagc ccattgagca aagcgatcat
     9721 caacgtaacc gcggccttca ggtcctggcg tatattcacc acgctttgcc ttcataacgt
     9781 tgccgggacc gtcgtgataa gcctgaagag cgtcacgcca gttaccaaac tgctggtata
     9841 tctttgccag atagcgcgcg ccagcgtcag cctgatattc ggggttttgc atttgctcat
     9901 cggtataacc catatcacgc catgtcccag gcatgacctg agtcaatcct acagccccgg
     9961 cggagcttac tgcggcaggg ttgtaagaag actccttggc acccagtgca gtcatcaacc
    10021 cttctggcac accgtaacgt gcgccagcct gctctaacaa atcacggtaa ttagccattt
    10081 actgccccaa agatggaaga tatccgtagc gattaatgaa gtcgattgac agctcggggt
    10141 gctgcttcag gtaatctata gaagcctgag gcgcttccac tcgcttgata ccgttttgct
    10201 gaacgtactt accaaccgcc tcattacgct tctggttgag cgtgttcagg atgacgccag
    10261 cgttgcgacg aaaggactcc tcgctctgcg agttctgcag cgaaccaaca gcctggtcga
    10321 gctttttgcc ctcggcatca gaaagtgcgc ccatgcctcg catggcctga accgctgtca
    10381 ggtatgcttg ggatttaaag gtatcaagtc gtgcctgagt gtctgcagcc tgtgagcctg
    10441 gaacgttggg gattactcca cgtaagcctg taatgctctt aagtgaagga gaactaacga
    10501 tatcgttcag agttaacatg ctggttgtga gggtgttgat gccgtctttg tagccatcat
    10561 ttagcgcttg ctgcttctgc tgcaactgct ggttgttggc tgctatgcgg ctctgtattt
    10621 cctggcgctt caggtcgtta gtttctgctg atagcatccg gtcaaggcgc ttattttcgt
    10681 tgttaatgcg gtttgtttct gcgtccagat taatgcgctg ctgacctaaa ttcgcctgga
    10741 tatcttgccc gcgcattgtg attgcctgat tccgagcggc ggtttgcgaa tccaggtcct
    10801 gaccgcgcat ggtaacctgg cgaccctgca ttttatcctg taggtcaaag tatttttcgg
    10861 gtccgagact gttcatcccc aggtgatcga caaattcgcc gaactgccgc gggttctgtt
    10921 ggtacatctg agcgacgtcc tgaggattaa cgccaacacg agctaactca ccggcgttgt
    10981 tttgcagcca tgattgcatt gcttctggag acgatgacgc aaggcgtgcg ccagccgcta
    11041 aggtgccgat agaattacgc tggtcttcat caatgaatcc catgccttta cgaacggatt
    11101 caatctggtc tggatattga gtagccaact gacgcaaagc accgcgatca ccagacgcat
    11161 aagcattagc gtacgcctgc tgaaattctt tctgccgctg agcctgcttt tcctgctgaa
    11221 acacccccgc aatacctgaa aggccttgca aagcagtcag cccaacattg ttagcgcctg
    11281 aacgctcaat atcattgttc tgcctgataa gctgaagcgt att
//
LOCUS       DAJTYF010000122        11123 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTYF010000122 DAJTYF010000000
VERSION     DAJTYF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.122"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="OO590_003378"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="OO590_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6638629.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTVLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="OO590_003379"
     CDS             complement(2005..2928)
                     /locus_tag="OO590_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6638630.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGHAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINTIRKSMPF"
     gene            3145..4488
                     /locus_tag="OO590_003380"
                     /pseudo
     CDS             3145..4488
                     /locus_tag="OO590_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="OO590_003381"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="OO590_003381"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6638631.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTLQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="OO590_003382"
     CDS             6508..6732
                     /locus_tag="OO590_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6638632.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="OO590_003383"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="OO590_003383"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6638633.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /locus_tag="OO590_003384"
     CDS             complement(7326..8306)
                     /locus_tag="OO590_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCR6638634.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="OO590_003385"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="OO590_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6638635.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="OO590_003386"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="OO590_003386"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6638636.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10983
                     /locus_tag="OO590_003387"
     CDS             10315..10983
                     /locus_tag="OO590_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6638637.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11001..>11123)
                     /locus_tag="OO590_003388"
     CDS             complement(11001..>11123)
                     /locus_tag="OO590_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6638638.1"
                     /translation="ISVIRQYIEQQQTPGQVENRALYPRPEGWGFTAHRIKSAI"
BASE COUNT         2866 a         2594 c         2800 g         2863 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg tgttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cctcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggccgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatacag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag tgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agcagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagact
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgt gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgtaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg ctgttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcactag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaagaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgctgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcagg
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact cgcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cccgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga ggaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgcgcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatattct ggttttttaa acaattactc tgatttaaaa
    10441 gaaacaacct cagcaacagg taaacctgtt ttacgttggg tagacccgag ttttgatcaa
    10501 agcaaatatg acagcatcgt ctggaaccca atcacttatt atccggtacc aaaaccttct
    10561 acccaggtag ggcagaaagt tctggataaa attttgaact ataccaacac cgaaatgaaa
    10621 gaagcgatag cgcagcgtaa accagtggtt accaccgctg ggccgcgtag tctgattttc
    10681 cgtggggcca ttaccggtgt agataccagc aaagaagggc tgcaattcta tgaagtggtt
    10741 cctgttgcat tagtggttgc ggggacgcaa atggctacag gccaccgtac catggatact
    10801 cgcctctatt ttgaaggtga gctgattgat gcagcgacta ataaaccggt tatcaaagtc
    10861 gttcgtcagg gcgaaggtaa agacctgaat aacgaaagta cgccaatggc tttcgaaaat
    10921 attaaacaag ttattgatga catggcgacc gatgccacca tgtttgacgt taacaaaaag
    10981 tagttccaga cgcgccatcg ttagatggcg ctttttatcc ggtgcgccgt aaaaccccat
    11041 ccttcagggc ggggatataa ggcgcggttt tccacctgac caggtgtttg ctgttgttca
    11101 atgtattggc ggatgacgga tat
//
LOCUS       DAJTYF010000123        11078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTYF010000123 DAJTYF010000000
VERSION     DAJTYF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.123"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            35..394
                     /locus_tag="OO590_003389"
     CDS             35..394
                     /locus_tag="OO590_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6638639.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(514..693)
                     /locus_tag="OO590_003390"
     CDS             complement(514..693)
                     /locus_tag="OO590_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6638640.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            767..2128
                     /gene="pabB"
                     /locus_tag="OO590_003391"
     CDS             767..2128
                     /gene="pabB"
                     /locus_tag="OO590_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6638641.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQKVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2132..2710
                     /locus_tag="OO590_003392"
     CDS             2132..2710
                     /locus_tag="OO590_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6638642.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2894..4258
                     /gene="sdaA"
                     /locus_tag="OO590_003393"
     CDS             2894..4258
                     /gene="sdaA"
                     /locus_tag="OO590_003393"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6638643.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDNLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4389..5987
                     /locus_tag="OO590_003394"
     CDS             4389..5987
                     /locus_tag="OO590_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6638644.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(5991..7541)
                     /gene="yoaE"
                     /locus_tag="OO590_003395"
     CDS             complement(5991..7541)
                     /gene="yoaE"
                     /locus_tag="OO590_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6638645.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENCDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            8004..8975
                     /gene="manX"
                     /locus_tag="OO590_003396"
     CDS             8004..8975
                     /gene="manX"
                     /locus_tag="OO590_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6638646.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFIPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9038..9838
                     /gene="manY"
                     /locus_tag="OO590_003397"
     CDS             9038..9838
                     /gene="manY"
                     /locus_tag="OO590_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6638647.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9851..10702
                     /gene="manZ"
                     /locus_tag="OO590_003398"
     CDS             9851..10702
                     /gene="manZ"
                     /locus_tag="OO590_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6638648.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10757..>11078
                     /locus_tag="OO590_003399"
     CDS             10757..>11078
                     /locus_tag="OO590_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HCR6638649.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIE"
BASE COUNT         2713 a         2806 c         2795 g         2764 t
ORIGIN      
        1 tcgtgccata tcgttataat ccctttgata ttagatgcaa attaaggtca tatatagcct
       61 tatcgataac atggttaatt ttaaggataa gaatatgcct gctgtaatag ataaagccct
      121 ggatttcatt ggtgccatgg atgtatcagc gccaacacca agttcgatga atgaaagcac
      181 ggcgaaggga atctttaaat atttaaaaga actgggagta cccgcgagtg ccgctgatat
      241 tacggcgcga gctgaccagg aaggctggaa ccctgggttc acggaaaaaa tggttggatg
      301 ggcaaaaaaa atggagacag gtgaacgctc tgtgattaaa aatcctgaat acttttcaac
      361 atatatgcag gaagaactaa aagcactggt ctgagttaaa tttatatcag cataaatggg
      421 tcaggacgct tttaatcaca tataaaaagc gtcctttttc tcacaatcaa atgatcatcg
      481 gcttctataa cgaaatcgat acggattagt gtgttactcg tcttcatcct caaaacgcgc
      541 cacgattagc tcgccggagt ggttggcacg cagctcttct gccaccagcg cgattgcctg
      601 gccgctactc atcccttgtg ccatcagttc ctggatccgc tcgacagctt tttgctgctg
      661 ttcatgggtg agtgaaggta aacctgcaaa cattgttaac tcctgctaaa ttgttggcgc
      721 taattatttc atgctacccg gcacatagcc agtagagtca ggactgatga agacgttatc
      781 tcccgctgtg attactttac cctggcgtca ggacgccgct gaattttatt tctcccgctt
      841 aagccacctg ccgtgggcga tgcttttaca ctccggctat gccgatcatc cgtatagccg
      901 ctttgatatt gtggtcgccg atccgatttg cactttaacc actttaggta aagaaaccgt
      961 tgttagtgaa agcgaaaaac gcacaacgac cactgatgac ccgctacagg tgctccagaa
     1021 ggtgctggat cgcgcagaca ttcgcccaac gcataacgaa gatttgccat ttcagggcgg
     1081 cgcactgggg ttgtttggct acgatctggg ccgccgtttt gagtcactgc cagaaattgc
     1141 ggaacaagat atcgttctgc cggatatggc agtgggtatc tacgattggg cgctcattgt
     1201 cgaccaccag cgtcatacag tttctttgct gagtcataat gatgtcaatg cccgtcgggc
     1261 ctggctggaa agccagcaat tctcgccgca ggaagatttc acgctcactt ccgactggca
     1321 atccaatatg acccacgagc agtacggcga aaaatttcgc caggtacagg aatatctgca
     1381 cagcggtgat tgctatcagg tgaatctcgc ccagcgtttt catgcgacct attctggcga
     1441 tgaatggcag gcattccttc agcttaatca ggccaaccgc gcgccattta gcgctttttt
     1501 acgtcttgaa cagggtgcaa ttttaagcct ttcgccagag cggtttattc tttgtgataa
     1561 tagtgaaatc cagacccgcc cgattaaagg cacgctacca cgcctgcccg atcctcagga
     1621 agatagcaaa caagcagaaa aactggcgaa ctcagcgaaa gatcgtgccg aaaatctgat
     1681 gattgtcgat ttaatgcgta atgatatcgg tcgtgttgcc gtagccggtt cggtaaaagt
     1741 accagagctc ttcgtggtgg aacccttccc tgccgtgcat catctggtca gcactataac
     1801 ggcgcgacta ccagaacagt tacacgccag cgatctgctg cgcgcagctt ttcctggtgg
     1861 ctcaataacc ggggctccga aagtacgggc tatggaaatt atcgacgaac tggaaccgca
     1921 gcgacgtaat gcctggtgcg gcagcattgg ctatttgagc ttttgcggca acatggatac
     1981 cagcattact atccgcacgc tgactgccat taacggacaa atatactgct ctgcgggcgg
     2041 tggaattgtc gccgatagcc aggaagaagc ggaatatcag gaaacttttg ataaagttaa
     2101 taagatatta cgccaactgg agaagtaaaa cgtggaatac cgtagcctga cgcttgatga
     2161 ttttttatct cgctttcaac ttttgcgccc gcaaattaac cgggaaaccc taaatcatcg
     2221 tcaggctgcc gtgttaatcc ccatcgtccg tcgaccacaa ccggggttgt tgctgactca
     2281 gcgttcaatt catctgcgta aacacgctgg acaagtggca ttccctggag gtgcagtcga
     2341 tgacacggac gcatcagtta tcgccgccgc gctgcgcgaa gctgaagaag aggtcgctat
     2401 accgccttcc gccgttgaag ttatcggcgt gctgccgccc gtcgatagcg taactggcta
     2461 tcaggtaacc ccagtggtcg gcattatccc gcccgatctg ccgtatcgcg ccagtgaaga
     2521 tgaagtctcg gcggtgtttg aaatgccgct cgcccaggca ttacatctgg gtcgttatca
     2581 ccctttagat atctaccgcc gtggcgattc acatcgggta tggctgtcct ggtacgaaca
     2641 gtattttgta tggggaatga ccgcaggcat aattcgtgag ctggcgctgc aaattggtgt
     2701 gaacccctga ctatacttat ctttacatct acaaaacact acttgagaca atcatcgcaa
     2761 tattagttaa atcgcggttt ttgattagtt taattcatgt gaatagttaa gccagtcgcc
     2821 gcgttccctc ttacactatg cgctgttatt agttcgttac tggaagtcca gtcaccttgt
     2881 caggagtatt atcgtgatta gtctattcga catgtttaag gtggggattg gtccctcatc
     2941 ttcccatacc gtagggccta tgaaggcggg taaacagttc gtcgataatc tggtcgaaaa
     3001 aggcttactg gatagcgtta ctcgcgttgc cgtggacgtt tatggttcac tgtcgctgac
     3061 gggtaaaggc caccacaccg atatcgccat tattatgggt cttgcaggta acgaacctgc
     3121 caccgtggat atcgacagta ttcccggttt tattcgcgac gtaaaagagc gcgaacgtct
     3181 gctgctggca cagggacggc atgaagtgga tttcccgcgc gacaacggga tgcgttttca
     3241 taacggcaac ctgccgctgc atgaaaacgg tatgcaaatc cacgcctata acggcgatga
     3301 agtcgtctac agcaaaactt attattccat cggcggcggt tttatcgtcg atgaagaaca
     3361 ctttggtcag gatgctgcca acgaagtgag cgtgccgtat ccgttcaaat ctgccaccga
     3421 actgctcgcg tactgtaatg aaaccggcta ttcgctgtct ggtctcgcta tgcagaacga
     3481 actggcgctg cacagcaaga aagagatcga cgagtatttc gcgcatgtct ggcaaaccat
     3541 gcaggcatgt atcgatcgcg ggatgaacac cgaaggtgta ctgccaggcc cgctgcgcgt
     3601 gccacgtcgt gcgtctgccc tgcgccggat gttagtttcc agcgataaac tgtctaacga
     3661 tccgatgaat gtcattgact gggtaaacat gtttgcgctg gcagttaacg aagaaaacgc
     3721 cgccggtggt cgtgtggtaa ctgcgccaac caacggtgcc tgcggtatcg ttccggcagt
     3781 gctggcttac tatgaccact ttattgaatc ggtcagccca gacatctata cccgttactt
     3841 tatggcagcg ggcgcgattg gtgcattgta taaaatgaac gcctctattt ccggtgcgga
     3901 agttggttgc cagggcgaag tgggtgttgc ctgttcaatg gctgctgcgg gtcttgcaga
     3961 actgctgggc ggtagcccgg aacaggtttg cgtggcggcg gaaattggca tggaacacaa
     4021 ccttggttta acctgcgacc cggttgcagg gcaggttcag gtgccgtgca ttgagcgtaa
     4081 tgccattgcc tctgtgaagg cgattaacgc cgcacggatg gctctgcgcc gcaccagtgc
     4141 accgcgcgtc tcgctggata aggtcatcga aacgatgtac gaaaccggta aggacatgaa
     4201 cgccaaatac cgcgaaacct cacgcggtgg tctggcaatc aaagtccagt gtgactaata
     4261 cttcttactc gcccatctgc aacggatggg cgaatttata cccgctttct cgtctgctgt
     4321 aatattcccc actacacttc cactgttgcg tcaggcgttt gtcgccatac gcttacaggg
     4381 tggcccgcat gcaaaaagca cagcggatca ttaaaaccta tcgccgtaat cgaatgattg
     4441 tttgtacgat ttgcgcactc gttacgctcg cttcgaccct gagcgtgcga tttatttcac
     4501 agcgtaactt aaatcaacaa cgggtagtac aattcgccaa tcacgctgta gaggaattag
     4561 ataaagtact gcttccccta caggcaggta gcgaagtctt gcttccgctg attggtctgc
     4621 cctgctctgt cgcccatttg ccattacgta aacaggcggc aaaactccaa actgtgcgat
     4681 ccattggcct ggtgcaagac ggcacacttt attgctccag catttttggt tatcgcaatg
     4741 tgcccgtcgt ggacattctg gctgaacttc ctgcaccgca accactttta cgcctgacga
     4801 tcgaccgtgc cctgattaaa ggcagtccgg ttttgattca atggacgcct gcagcaggca
     4861 gtagcaatgc tggggtcata gagatgatta acatcgactt actgacggca atgctccttg
     4921 agccacaact gccgcaaatc agtagcgcca gcctgacggt ggacaaacgg catttgctct
     4981 atggtaatgg gctggtagat tcccttccgc aacctgaaga caatgaaaac tatcaggttt
     5041 cttcgcaaag ctttcctttt accattaacg taaatggtcc gggggctacg gcgctggcat
     5101 ggcactatct tccaacacaa ttaccgctgg cggtgctgct aagtttactg gtgggctata
     5161 tcgcctggct ggcgaccgct taccggatga gcttttcccg cgaaattaat ctgggcctgg
     5221 cgcaacatga gttcgaattg ttctgtcagc ctttgcttaa tgcgcgcagc cagcaatgta
     5281 ttggtgtaga gattttgctg cgctggaata atccgcgtca gggctggatt tcaccggatg
     5341 tgtttattcc tatcgcggaa gaacatcatt taattgtgcc actgacccgc tatgtgatgg
     5401 cagaaaccat tcgtcagcgc catgttttcc cgatgagtag tcagtttcat gttggcatta
     5461 acgtcgcacc cagccatttt cgccgtggtg tgctgataaa agatctcaat cagtactggt
     5521 ttagcgctca cccgattcag caactgatcc tcgaaatcac cgaacgcgat gccttactgg
     5581 atgttgatta tcggattgcc cgcgagctgc atcgtaaaaa cgtcaaactg gcgattgatg
     5641 acttcggcac cggcaacagc tcgttttcct ggcttgaaac attacgtcct gacgtgctga
     5701 aaattgataa gtcatttacc gcagctatag gttctgacgc ggttaactcg acggtgaccg
     5761 atatcatcat cgcgctgggg caaagactga atattgaact ggtggcggag ggcgtggaaa
     5821 cgcaagaaca ggcgaagtat ttgcgccgtc atggcgtgca tattttgcaa gggtatttgt
     5881 acgcacagcc gatgccgcta cgtgattttc ccaaatggct ggcgggcagc caaccgccgc
     5941 ccgcccggca taatggacat atcacgccca ttatgtcgtt acgttaacgc ttactcatct
     6001 tcatcgtgcg ccggttgctc tttaacaatg cgaaccagat caacacgata atcattggct
     6061 tcaaggatgg tgatatgcag tggccctaca tcaatcacat cgcccacacg gggaatgtga
     6121 ccatttgccg agatcacgag gcccgcgacc gtcgcgatat catcgtcatc ggcaaggtgc
     6181 tcaacatcaa gcgcctgctg caaggcatgc aaatctgtac cgccttttac cagccagccg
     6241 ttaccatcgg taatgatttc cggcgtttcg tcagcgtccg ggaattcacc cgcaatggct
     6301 tccagcacat ccagcggcgt gaccaagcct tgtaccacac caaactcgtt ggtcacgata
     6361 acaaagctcc cgcgagcgcg acgcagcacg cccagcaggt tgatcggatc gagggtttcc
     6421 gggacgataa tcgccggaga cgccgaagca atcgccgcca catcaacgcc ctcttccagc
     6481 gccaccagca gttctttagc acgtacgata ccgatgattt catccagttc accgcgacat
     6541 accgggaaca gactgtgcgg tgaagagagc agttgctcgc ggatttcatc gaccccgaga
     6601 ttagcgtcaa cccagcttat ttcaccgcgc ggcgtcatga tcccgcgcag agaacgcgac
     6661 gccagcgtca gtacgccgtt aatcatgtac cgttcttctt cggcaaatgc accttccggg
     6721 atcggcatcg ggttatcggc atcgtgctga acattggcct gacgtttccc gcccatcaaa
     6781 cgcaggatgg catcggcagt acgcgctcgc agcggcaaag tcgactggtg acgaataaag
     6841 ttgcgacgcg caatctggtt aaacacttcg ataatgatcg agaagccaat cgcggcatac
     6901 aggtaacctt tcggaatgtg gaaaccgaaa ccttctgcca ccagactcag accaatcatt
     6961 aacaggaagc tcagacagag caccaccacc gtggggtgct ggttaacgaa tcgcgtcagc
     7021 ggtttcgatg ccagcaacat aaccgccatc gcaatcacta ccgccgccat catcaccggc
     7081 agatggttaa ccatccctac tgcagtaatt accgcatcca acgagaagac ggcgtcaagg
     7141 atgacaatct gtgtgacgac cacccagaaa ctggcgtagc ctttaccgtg gccggaatca
     7201 tgatcgcagt tttccagccg ttcatgcagt tcggttgttg ctttgaacag caagaatatc
     7261 cccccgaaca acataatcag atcgcgtccg gagaaggaga aatccatgac ggtaaatagc
     7321 ggtttggtca gcgtgaccat ccatgaaatc agcgacagca gccccagacg cataatcagc
     7381 gccagtgata accccagcaa acgcgcttta tcgcgttgtt ttggcggcag tttgtcagca
     7441 agaatggcga tgaagaccag gttatcgata cccagcacaa tttcgagaac aacaagcgtg
     7501 agtagccccg cccaaattga ggggtccatt aagaattcca tgacaagctc ctgcttaagg
     7561 aatagctatt cgacgccaga aataatgcag gcgtaacgac aaaatgcaaa cgaaaggtgc
     7621 ggcatagagt gccagaaagg caggcgttaa aaggcctgat gctgaaatga cgtcggtgac
     7681 gatccatact gcgggctact gccctatact ccatggttgt taaacgggag ttaaacatat
     7741 cagagacgcc tctgatttgg caaagattta ccttcctttg caaacgaatg tgacaaggat
     7801 attttacctt tcgaaatttc tgataatcga aagttaaatt acggatcttc atcacataaa
     7861 ataatttttt cgatatctaa aataaatcgc gaaacgcagg ggtttttggt tgtagccctt
     7921 atctgaatcg attcgattgt ggacgacgat tcaaaaatac atctggcacg ttgaggtgtt
     7981 aacgataata aaggaggtag caagtgacca ttgctattgt tataggcaca catggttggg
     8041 ctgcagagca gttgcttaaa acggcagaaa tgctgttagg cgagcaggaa aacgtcggct
     8101 ggatcgattt cattccaggt gaaaatgccg aaacgctgat tgaaaaatac aacgctcagt
     8161 tggcaaaact cgacaccact aaaggcgtgc tgtttctcgt tgatacatgg ggaggcagcc
     8221 cgttcaatgc tgccagccgc attgtcgtcg acaaagagca ttatgaagtc attgcaggcg
     8281 ttaacattcc aatgctcgtg gaaacgttaa tggcccgtga tgatgacccg agctttgatg
     8341 aactggttgc gctggcagta gaaacaggcc gtgaaggcgt gaaagcactg aaagccaaac
     8401 cggttgaaaa agccgcgcca gcacccgctg ccgcagcacc aaaagcggct ccaactccgg
     8461 caaaaccaat ggggccaaac gactacatgg ttattggcct tgcgcgtatc gacgaccgtc
     8521 tgattcacgg tcaggtcgcc acccgctgga ccaaagaaac caatgtctcc cgtattattg
     8581 ttgttagtga tgaagtggct gcggataccg ttcgtaagac actgctcacc caggttgcac
     8641 ctccgggcgt aacagcacac gtagttgatg ttgccaaaat gattcgcgtc tacaacaacc
     8701 cgaaatatgc tggcgaacgc gtaatgctgt tatttaccaa cccaacagat gtagagcgtc
     8761 tcgttgaagg cggcgtgaaa atcacctctg ttaacgtcgg tggtatggca ttccgtcagg
     8821 gtaaaaccca ggtgaataac gcggtttcgg ttgatgaaaa agatatcgag gcgttcaaaa
     8881 aactgaatgc acgcggtatt gagctggaag tccgtaaggt ttccaccgat ccgaaactga
     8941 aaatgatgga tctgatcagc aaaatcgata agtaacgtat tgtgttgatt atcattcagt
     9001 tttcacactt aagtcttacg taaacaggag aagtacaatg gagattacca ctcttcaaat
     9061 tgtgctggta tttatcgtag cctgtatcgc aggtatggga tcaatcctcg atgaatttca
     9121 gtttcaccgt ccgctaatcg cgtgtaccct ggtgggtatc gttcttgggg atatgaaaac
     9181 cggtattatt atcggtggta cgctggaaat gatcgcgctg ggctggatga acatcggtgc
     9241 tgcagttgcg cctgacgccg ctctggcttc tatcatttct accattctgg ttatcgcagg
     9301 tcatcagagc attggtgcag gtatcgcact ggcaatccct ctggccgctg cgggccaggt
     9361 actgaccatc atcgttcgta ctattaccgt tgctttccag cacgctgcgg ataaggctgc
     9421 tgataacggc aacctgacag cgatttcctg gatccacgtt tcttctctgt tcctgcaagc
     9481 aatgcgtgtg gctattccgg ccgtcatcgt tgcgctgtct gttggtacca gcgaagtaca
     9541 gaacatgctg aatgcgattc cggaagtggt gaccaatggt ctgaatatcg ccgggggcat
     9601 gatcgtggtg gttggttatg cgatggttat caacatgatg cgtgctggct acctgatgcc
     9661 gttcttctac ctcggcttcg taaccgcagc attcaccaac tttaacctgg ttgctctggg
     9721 tgtgattggt actgttatgg cagtgctcta catccaactt agcccgaaat acaaccgcgt
     9781 agccggtgcg cctgctcagg cagctggtaa caacgatctc gataacgaac tggactaaca
     9841 ggtgagcgaa atggttgata caactcaaac taccaccgag aaaaaactca ctcaaagtga
     9901 tattcgtggc gtattcctgc gttctaacct cttccagggt tcatggaact tcgaacgtat
     9961 gcaggcactg ggtttctgct tctctatggt accggcaatt cgtcgcctct accctgagaa
    10021 caacgaagct cgtaaacaag ctattcgccg tcacctggag ttctttaaca cccagccgtt
    10081 cgtggctgca ccgattctcg gcgtaaccct ggcgctggaa gaacagcgtg ctaatggcgc
    10141 agagatcgat gacggtgcta tcaacggtat caaagtcggt ttgatggggc cgctggctgg
    10201 tgtaggcgac ccgatcttct ggggaaccgt acgtccggta tttgcagcac tgggtgccgg
    10261 tatcgcgatg agcggcagcc tgttaggtcc gctgctgttc ttcatcctgt ttaacctggt
    10321 gcgtctggca acccgttact acggcgtagc ttatggttac tccaaaggta tcgatatcgt
    10381 taaagatatg ggtggtggct tcctgcaaaa actgacggaa ggggcgtcta tcctcggcct
    10441 gtttgtcatg ggggcattgg ttaacaagtg gacacatgtc aacatcccgc tggtggtttc
    10501 gaccatcacc gggcaggatg gacagacccg tgtcaccacc gtgcaaacta ttcttgacca
    10561 gttaatgcca ggccttgtac cactgctgct gacatttgct tgtatgtggc tgctgcgcaa
    10621 aaaagttaac ccgctgtgga tcatcgttgg cttcttcgtc atcggtatcg ctggttacgc
    10681 ttgcggcctg ctgggactgt aagactgttg tacactaccg gggccttttg gccccgtttt
    10741 tttatctgga ggattaatga caatcacgga cctggtactg attcttttca tcgccgcact
    10801 cctggccttc gcgatctacg atcagttcat catgccccgc cgtaacggcc ccaccctgct
    10861 ggcaattcct ttgctccgcc gtggtcgcat cgatagcgtt atcttcgtcg gattgattgt
    10921 cattcttatc tataacaacg tcacgaatca tggcgcgcta ataacaacat ggttattaag
    10981 tgcactggct ctgatgggtt tttatatatt ctggatccgc attccgaaga tcatctttaa
    11041 acaaaaaggt tttttcttcg ccaatgtctg gattgaat
//
LOCUS       DAJTYF010000124        10869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTYF010000124 DAJTYF010000000
VERSION     DAJTYF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.124"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..924
                     /locus_tag="OO590_003400"
     CDS             <1..924
                     /locus_tag="OO590_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6638650.1"
                     /translation="LGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAP
                     PALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPVKPRP
                     QLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAI
                     PFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAV
                     AFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDT
                     THSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="OO590_003401"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="OO590_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6638651.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="OO590_003402"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="OO590_003402"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6638652.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="OO590_003403"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="OO590_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6638653.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="OO590_003404"
     CDS             4447..4806
                     /locus_tag="OO590_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6638654.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="OO590_003405"
     CDS             complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="OO590_003405"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6638655.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6060..6250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6315..8159
                     /gene="btuB"
                     /locus_tag="OO590_003406"
     CDS             6315..8159
                     /gene="btuB"
                     /locus_tag="OO590_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6638656.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALDHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8104..8961
                     /gene="murI"
                     /locus_tag="OO590_003407"
     CDS             8104..8961
                     /gene="murI"
                     /locus_tag="OO590_003407"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6638657.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            9335..10869
                     /locus_tag="OO590_003408"
     rRNA            9335..10869
                     /locus_tag="OO590_003408"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2539 a         2605 c         2992 g         2733 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactggtat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcgc ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgcc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tctacattgc gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttgctt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt gataggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tattttacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaatcaa cataatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggca cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atttgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcatcgcgc agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag
     5581 cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg
     5641 gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag
     5701 ttcactggcg gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat
     5761 gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg
     5821 actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg
     5881 caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg
     5941 ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat
     6001 gtccagatct tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc
     6061 tgtagcatcc acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctagagctg
     6121 acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg
     6181 aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg
     6241 tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt
     6301 gtggatgctt tacaatgatt aaaaaagctt cgttgctgac ggcgtgttcc gtcacggcat
     6361 tttccgcttg ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg
     6421 aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc
     6481 gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc
     6541 aaaacggcgg ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg
     6601 tgctggtgtt aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc
     6661 ttagccagtt ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg
     6721 ctgtttatgg ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac
     6781 ccggaacgga aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta
     6841 cgcagcaaca actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc
     6901 atggttatga tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg
     6961 gttttttaag taaaacgctt tatggggcgc tggatcataa ctgtactgat gcctggagcg
     7021 gctttgttcg cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg
     7081 gttcaccgtt gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct
     7141 ataacggcga actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca
     7201 actacgatcc ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat
     7261 acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg
     7321 actggcagaa acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac
     7381 gtaataccgg catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg
     7441 cagcacgcag cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg
     7501 gttgggaatt catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac
     7561 caaatctggg gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca
     7621 aacagtggga aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat
     7681 atcgtaacga tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg
     7741 aagggaaagc gcggattaag ggcgtcgagg cgacagccaa ttttgatacc ggaccactga
     7801 cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt
     7861 tacgccgtgc taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact
     7921 ggggtattac ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt
     7981 atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca
     8041 cctctcacct gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag
     8101 tctatggcta ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa
     8161 ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag
     8221 atccggcatc tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg
     8281 tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg
     8341 caagaacgtt atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca
     8401 cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa
     8461 cctgctgcac gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt
     8521 aaacgttctt atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg
     8581 ctgggctcgg cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct
     8641 ctggatgcac taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc
     8701 gttgtattgg gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca
     8761 gagggaaccc ggctggtgga ttctggcgca gcgattgctc gccgaacggc ttggttgtta
     8821 gaacatgaag ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg
     8881 acgccagaag ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa
     8941 aaactggcag ttttaggctg atttggttga atgttgcgcg gtcggaaaat tattttaaat
     9001 ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg
     9061 caaacacgcc gccgggtcag cggggttctc ctgagaattc cggcagagaa agcaaaaata
     9121 aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc tcgcgccgct gagaaaaagc
     9181 gaagcggcac tgctctttaa caatttatca gacaatctgt gtgggcactc gaagatacgg
     9241 attcttaacg tcgcaagacg aaaaatgaat accaagtctc aagagtgaac acgtaattca
     9301 ttacgaagtt taattctttg agcatcaaac ttttaaattg aagagtttga tcatggctca
     9361 gattgaacgc tggcggcagg cctaacacat gcaagtcgaa cggtaacagg aagcagcttg
     9421 ctgtttcgct gacgagtggc ggacgggtga gtaatgtctg ggaaactgcc tgatggaggg
     9481 ggataactac tggaaacggt agctaatacc gcataacgtc gcaagaccaa agagggggac
     9541 cttcgggcct cttgccatcg gatgtgccca gatgggatta gctagtaggt ggggtaacgg
     9601 ctcacctagg cgacgatccc tagctggtct gagaggatga ccagccacac tggaactgag
     9661 acacggtcca gactcctacg ggaggcagca gtggggaata ttgcacaatg ggcgcaagcc
     9721 tgatgcagcc atgccgcgtg tatgaagaag gccttcgggt tgtaaagtac tttcagcggg
     9781 gaggaaggga gtaaagttaa tacctttgct cattgacgtt acccgcagaa gaagcaccgg
     9841 ctaactccgt gccagcagcc gcggtaatac ggagggtgca agcgttaatc ggaattactg
     9901 ggcgtaaagc gcacgcaggc ggtttgttaa gtcagatgtg aaatccccgg gctcaacctg
     9961 ggaactgcat ctgatactgg caagcttgag tctcgtagag gggggtagaa ttccaggtgt
    10021 agcggtgaaa tgcgtagaga tctggaggaa taccggtggc gaaggcggcc ccctggacga
    10081 agactgacgc tcaggtgcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc
    10141 acgccgtaaa cgatgtcgac ttggaggttg tgcccttgag gcgtggcttc cggagctaac
    10201 gcgttaagtc gaccgcctgg ggagtacggc cgcaaggtta aaactcaaat gaattgacgg
    10261 gggcccgcac aagcggtgga gcatgtggtt taattcgatg caacgcgaag aaccttacct
    10321 ggtcttgaca tccacggaag ttttcagaga tgagaatgtg ccttcgggaa ccgtgagaca
    10381 ggtgctgcat ggctgtcgtc agctcgtgtt gtgaaatgtt gggttaagtc ccgcaacgag
    10441 cgcaaccctt atcctttgtt gccagcggtc cggccgggaa ctcaaaggag actgccagtg
    10501 ataaactgga ggaaggtggg gatgacgtca agtcatcatg gcccttacga ccagggctac
    10561 acacgtgcta caatggcgca tacaaagaga agcgacctcg cgagagcaag cggacctcat
    10621 aaagtgcgtc gtagtccgga ttggagtctg caactcgact ccatgaagtc ggaatcgcta
    10681 gtaatcgtgg atcagaatgc cacggtgaat acgttcccgg gccttgtaca caccgcccgt
    10741 cacaccatgg gagtgggttg caaaagaagt aggtagctta accttcggga gggcgcttac
    10801 cactttgtga ttcatgactg gggtgaagtc gtaacaaggt aaccgtaggg gaacctgcgg
    10861 ttggatcac
//
LOCUS       DAJTYF010000125        10815 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTYF010000125 DAJTYF010000000
VERSION     DAJTYF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.125"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            93..1178
                     /locus_tag="OO590_003409"
     CDS             93..1178
                     /locus_tag="OO590_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6638658.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1249..1857
                     /locus_tag="OO590_003410"
     CDS             1249..1857
                     /locus_tag="OO590_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6638659.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1955..3346
                     /gene="selA"
                     /locus_tag="OO590_003411"
     CDS             1955..3346
                     /gene="selA"
                     /locus_tag="OO590_003411"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6638660.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVTHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3343..5187
                     /gene="selB"
                     /locus_tag="OO590_003412"
     CDS             3343..5187
                     /gene="selB"
                     /locus_tag="OO590_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6638661.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5377..6528
                     /gene="yiaY"
                     /locus_tag="OO590_003413"
     CDS             5377..6528
                     /gene="yiaY"
                     /locus_tag="OO590_003413"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6638662.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6693..8231
                     /gene="aldB"
                     /locus_tag="OO590_003414"
                     /pseudo
     CDS             6693..8231
                     /gene="aldB"
                     /locus_tag="OO590_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
     gene            complement(8450..8605)
                     /locus_tag="OO590_003415"
     CDS             complement(8450..8605)
                     /locus_tag="OO590_003415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638663.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            8776..9099
                     /locus_tag="OO590_003416"
     CDS             8776..9099
                     /locus_tag="OO590_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6638664.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9105..10241
                     /locus_tag="OO590_003417"
     CDS             9105..10241
                     /locus_tag="OO590_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6638665.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10238..>10815)
                     /locus_tag="OO590_003418"
     CDS             complement(10238..>10815)
                     /locus_tag="OO590_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6638666.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2578 a         2659 c         2976 g         2602 t
ORIGIN      
        1 atcgccgcac tttctgaccg aatttcccgg ctggagcatc aactcgccgc cgagaaaaag
       61 actgactatt ccacgttccc ggagatctaa ttatggatct attgattgtt ttaacttacg
      121 tggcgctggc gacggcggcg ctgggaggcg tgtttctggt gagtggtttg attttgttga
      181 tgaactacaa ccacccttac acttttaccg cgcaaaaggc agtgatagcg atccctatca
      241 cgccacaggt gacgggaatt gttactgaag tcactgacaa gaataatcag cttattcaaa
      301 agggcgaggt gctttttaag ctcgacccgg ttcgttacca ggcgcgagtt gacagacttc
      361 aggctgacct gatgacggcg acgcataata taaagacgct gcgtgcgcag ctcactgaag
      421 cgcaggccaa caccacccag gtttcagcgg agcgcgaccg tctgtttaaa aattatcaac
      481 gttacctgaa aggcagccag gcggcggtga atccgttctc ggaacgtgac atcgacgatg
      541 cgcggcaaaa tttcctcgcg caggatgcgt tagtgaaagg ctcggtggcg gagcaggcgc
      601 agatccagag tcagctcgac agtatggtca acggcgagca atcgcagatt gtgagcttaa
      661 gagcgcaact tactgaagca aaatataatc ttgagcagac tgtcattcgc gcaccaagca
      721 atggctacgt cactcaggta ctgatccgcc caggtacata cgcagctgcc ttgccgctgc
      781 gtccggtgat ggtcttcatc cccgagcaaa aacggcaaat tgtcgcccaa tttcggcaaa
      841 actcgctgtt acgtctgaaa cctggcgatg atgcggaagt ggtgtttaac gcgctacctg
      901 ggcaggtgtt ccacggcaaa ttgactagta ttttacctgt cgtgccaggc ggttcttatc
      961 aggcgcaggg ggtattgcaa tcattaacgg tcgttcccgg cacggacggt gtgctgggaa
     1021 ccattgaact ggaccctaac gatgatatcg atgccttacc cgacggcatc tacgcccagg
     1081 tggcggttta ctccgaccat ttcagccatg tttcggtgat gcggaaagtg ctgctaagaa
     1141 tgaccagttg gatgcattat ctttatttgg atcattgaga cttatatcac ctgtgttttg
     1201 cgtgagcctg agccatactg atatttttcc tcgcacaaag gactcggcat gaaactcgtc
     1261 ggtagctaca ccagcccgtt tgtacgcaaa ctttctattc tgttgttaga aaagggcata
     1321 actttcgaat ttattaatga actgccctat aacgcggata acggcgtggc gcaatttaac
     1381 ccgttaggga aagtgccggt gctggtgacc gaagagggcg aatgctggtt tgattcgccg
     1441 atcatcgctg aatatattga gttaatgaat gtcgctccgg cgatgttgcc gcgcgatccg
     1501 ctggagtcgt tgcgggtgcg caaaattgag gcgctggcgg atggcattat ggatgccggg
     1561 ctggtatcgg tgcgtgaaca ggcgcgtccg gcggcgcagc agtctgaaga tgaattgtta
     1621 cgccagcggg agaaaatcaa ccgcagtctg gatgtgctgg aaggatatct ggtcgatggc
     1681 acactcaaaa ccgatacggt caatctggcg actatcgcca ttgcctgtgc tgtcggatat
     1741 ctcaatttcc gccgcgttgc gccaggttgg tgtgtcgacc gcccacactt agtcaaactg
     1801 gtcgaaaacc tgtttagccg cgaaagtttt gctcgcaccg aaccgccaaa ggcttgatgc
     1861 gcgatatgtc ctcctgaccc atctcacgtt acaatccgtg gttatgttaa acgcccttct
     1921 ccgtgtgaga gggccttgat cagccaggtt tcctatgaca accgaaacgc gttccctcta
     1981 tagtcaactt ccggctattg atcgcttatt gcgcgatagc tccttccttt ctttgcgtga
     2041 tacttatggt cacacccgcg tggtggaatt gttgcgtcag atgctcgacg aagcgcgaga
     2101 agtgattcgt ggcagccaga cgctgcctgc gtggtgtgaa aactgggcgc aagaagtcga
     2161 tgcccggttg acgaaagagg cgcagagcgc gctgcgtccg gtgatcaacc tgacgggaac
     2221 cgtgctgcat accaaccttg ggcgagcttt acaggcggaa gccgcggtgg aagccgttgc
     2281 gcaggctatg cgttcgccag tgaccctcga gtacgatctg gacgacgccg gacgcggaca
     2341 tcgcgatcgg gcgctggcgc agctgctgtg ccgtattacg ggggcggaag atgcctgtat
     2401 cgtcaataac aatgcggcgg cggtgttatt gatgttggcg gccactgcca gcgggaaaga
     2461 ggtggtggta tctcgcggcg aactggtgga gattggcggc gcgtttcgta ttcccgatgt
     2521 tatgcgtcag gcaggctgca ccctacacga agtagggacc accaaccgca cgcacgcgaa
     2581 tgattatcgt caggcggtga atgaaaatac cgcactgttg atgaaagtac ataccagtaa
     2641 ctacagcatt caggggttca ccaaagcgat agacgaagct gaactggtgg cgctcggcaa
     2701 agagctggat gttccagtgg tgactgattt aggcagtggc tcgctggtcg atcttagcca
     2761 gtacggtttg ccgaaagagc caatgccgca ggagttgatt gcggcgggcg tcagtctggt
     2821 gagcttctcc ggcgacaagt tgttaggcgg gccgcaggca gggattatcg ttggtaaaaa
     2881 agagatgatc gcccgcctgc aaagccaccc gctgaagcgt gcattacgcg cggataaaat
     2941 gaccctcgcg gcgctggaag ccacgttgcg tctttattta caccctgaag ctctgagtga
     3001 aaaattaccg accctgcgcc tgcttacccg cagcgcagag gtcattcaaa tccaggcaca
     3061 acgtttacag gctccccttg tcacacatta cggcgcggag tttgcggtac aggttatgcc
     3121 atgtctttcg cagattggca gtggttcgct gccggttgat cgcctgccga gcgcggcatt
     3181 aacgtttaca ccccatgatg gacgcggtag ccaccttgag tcattagccg cccgctggcg
     3241 tgaattgcca gtgccggtga ttggtcgtat ttatgacgga cgattgtggc tggatttacg
     3301 ctgccttgaa gatgagcaac ggtttttgga gatgttgttg aaatgattat tgcgactgcc
     3361 ggacacgttg accacggcaa aacaacctta ttgcaggcga ttactggcgt aaatgctgac
     3421 cgtctgccgg aagaaaaaaa gcgcggcatg accatcgatc tcggctatgc ctactggccg
     3481 cagccggatg gtcgcgtgcc tggttttatc gacgttcccg gtcatgaaaa gtttctttcc
     3541 aacatgctgg cgggcgttgg tggtatcgat cacgcgctgt tggtggtggc gtgcgatgac
     3601 ggcgtgatgg cacagacccg tgagcatctg gcgattttgc agctgaccgg taacccgatg
     3661 ctgacagtgg cgctgaccaa agccgatcgt gtggacgaag cgcgtgttga tgaggttgaa
     3721 cgccaggtaa aggaggttct gcgggaatac ggttttgctg aggcaaaact gtttatcacc
     3781 gcagcaaccg aaggtcgggg aattgatgcc ctgcgcgagc atctgcttca gttgccggaa
     3841 cgcgagcacg ccagccaaca tagtttccgc ctcgcgattg accgcgcatt taccgtaaaa
     3901 ggtgccgggc tggtcgtcac cggtacggcg ttaagcgggg aagtgaaggt aggcgattca
     3961 ctctggctga ctggtgtaaa taaaccgatg cgtgtacgtg cgctgcatgc gcaaaaccag
     4021 ccaacagaaa ccgccaatgc cgggcagcgt atcgcgcttg acatcgcggg tgatgcggaa
     4081 aaagagcaga ttaaccgtgg cgactggctg cttgccgatg tgccgccaga gccgttcaca
     4141 cgggtgattg tcgagcttca aacccatgca ccgctgaccc agtggcagcc gctgcatatt
     4201 caccacgccg ccagccacgt cacgggacgc gtttcactgc tggaagataa ccttgctgaa
     4261 ctggtcttcg acaccccgtt atggctggca gataacgacc gcctggtatt gcgcgatatc
     4321 tctgcccgca acacgctggc cggagcgcgc gtcgtgatgc ttaacccgcc gcgtcgcggt
     4381 aaacgtaaac cagagtatct gcaatggctg gcttccctcg ctagggcaca gaatgatagc
     4441 gaagcgttag ccgttcacct gcaacgcggc gcggttaatc ttcctgattt cgcctgggcg
     4501 cgccagctca acggcgaagg gatgcgcgaa ctgctgcaac agcctggcta tattcaggct
     4561 ggttatagct tgttgaatgc gccggttgcc gctcgctggc aacggaaaat tctcgacaca
     4621 ttagcgactt atcatgagca acatcgcgat gaacctgggc ctgggcgcga acgtctgcga
     4681 cgtatggcgt tgccaatgga agatgaagcg ctagtactgt tgctgattga aaagatgcgc
     4741 gaaagcggcg acatcctcag tcatcacggt tggctgcatc tgccggatca caaagcgggc
     4801 ttcagcgaag agcagcaggc catctggcaa aaagcagagc cgctgtttgg tgacgagccg
     4861 tggtgggtgc gtgatctggc aaaagagacg ggtaccgacg agcaggcaat gcgcctgacg
     4921 ctacgccagg cggcgcagca aggaataatt accgcgatcg tcaaagatcg ttattaccgt
     4981 aacgatcgga ttgtcgaatt tgccaatatg atccgcgatc tcgatcagga gtgtggttca
     5041 acctgcgcgg cggatttccg cgatcgctta ggcgtaggcc gaaagctggc aattcagatt
     5101 ctggaatatt ttgaccgcat tggctttacg cgtcgtcgtg gaaatgatca tttattgcgc
     5161 gacgcattat tatttccgga aagataagga aatgattaat taatttttaa aataaattaa
     5221 tacaaatttc ttatgatttt aaataaagca cattgtttaa tgaatacaat gtgcttttta
     5281 ttagattaat tttgataatt aataataatg atcatgtttt cgaatgaaaa tcctcagtaa
     5341 gctgcccacc cttttttaca ctttcaggag tgtgttatgg catcttcaac tttctttatt
     5401 ccttctgtga atgtcatcgg cgctgattca ttgactgatg caatgaatat gatggcagat
     5461 tatggattta cccgtacctt aattgtcact gacaatatgt taacgaaatt aggtatggcg
     5521 ggtgatgtgc aaaaagcact ggaagaacgc aatattttta gcgttattta tgatggcacc
     5581 caacctaacc ccaccacgga aaacgtcgcc gcaggtttga aattacttaa agaaaataat
     5641 tgcgatagcg tgatctcctt aggcggtggt tctccacacg actgcgcaaa aggtattgcg
     5701 ctggtggcag ccaatggcgg cgatattcgc gattacgaag gcgttgaccg ctctgcaaaa
     5761 ccgcagctgc cgatgatcgc catcaatacc acggcgggta cggcctctga aatgacccgt
     5821 ttctgcatca tcactgacga agcgcgtcat aacaaaatgg cgattgttga taagcacgtg
     5881 actccgctgc tttctgtcaa tgactcctct ctgatgattg gtatgccgaa gtcactgacc
     5941 gccgcaacgg gtatggatgc cttaacgcac gctatcgaag cgtatgtttc tattgccgcc
     6001 acgccgatca ctgacgcttg tgcactgaaa gccgtgacca tgattgccga aaacctgccg
     6061 ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag caatggctta tgcccagttc
     6121 cttgccggta tggcgttcaa taatgcttct ctgggttatg ttcatgcgat ggcgcaccag
     6181 ctgggcggtt tctacaacct gccacacggt gtatgtaacg ccgttctgct gccgcatgtt
     6241 caggtattca acagcaaagt cgccgctgca cgtctgcgtg actgtgccgc tgcaatgggc
     6301 gtgaacgtga caggtaaaaa cgacgcggaa ggtgctgaag cctgcattaa cgccatccgt
     6361 gaactggcga agaaagtgga tatcccggca ggcctacgcg acctgaacgt gaaagaagaa
     6421 gatttcgcgg ttctggcgac taatgccctg aaagatgcct gtggctttac taacccgatc
     6481 caggcaactc acgaagaaat tgtggtgatt tatcgcgccg caatgtaatc atcattccaa
     6541 caacggctgg caaattgtca gccgcttttt caactatctc tgtaatcctt gcccgtaaat
     6601 tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga tttcgccagt cacgtctacc
     6661 cttgttatac ctcacaccgc aaggagacga tcatgaccaa taatccccct tcagcacaga
     6721 ttaagcccgg cgagtatggt ttccccatca agttaaaagc ccgctacgac aactttattg
     6781 gcggtgaatg ggtagcccct gccgatggtg agtattacca gaacctgacg ccggtgaccg
     6841 ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga catcgatctg gcgctggatg
     6901 ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt gcaggatcgc gcggcgattc
     6961 tgtttaagat tgccgatcga atggaacaaa acctcgagct gttagcgaca gctgaaacct
     7021 gggataacgg caaacccatt cgcgaaacca gtgctgcgga tgtaccgctg gcgattgacc
     7081 atttccgcta tttcgcctcg tgtattcggg cgcaggaagg tgggatcagt gaagttgata
     7141 gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt ggtggggcag attatcccgt
     7201 ggaacttccc gctgctgatg gcgagctgga aaatggctcc cgcgctggcg gcgggcaact
     7261 gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt actgctgcta atggaaattg
     7321 tcggtgattt actgccgccg ggcgtggtga acgtggtcaa tggcgcaggt ggggaaattg
     7381 gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc gtttaccggc tcaacggaag
     7441 tgggccaaca aattatgcaa tacgcaacgc aaaacattat tccggtgacg ctggagttgg
     7501 gcggtaagtc gccaaatatc ttctttgctg atgtgatgga tgaagaagat gcctttttcg
     7561 ataaagcgct ggaaggcttt gcactgtttg cctttaacca gggcgaagtt tgcacctgtc
     7621 cgagtcgtgc cttagtgcag gagtcaatct acgaacgctt tatggaacgc gccatccgcc
     7681 gtgtcgaaag cattcgcagc ggtaacccgc tcgacagcgt gacgcaaatg ggcgcgcagg
     7741 tttctcacgg gcaactggaa accatcctca actacattga tatcggtaaa aaagagggcg
     7801 ctgacgtgct cacaggcggg cggcgcaagc tgctggaagg tgaactgaaa gacggctact
     7861 acctcgaacc gacgattctg tttggtcaga acaatatgcg ggtgttctag gaggagattt
     7921 ttggcccggt gctggcggtg accaccttca aaacgatgga agaagcgctg gagctggcga
     7981 acgatacgca atatggcttg ggcgcgggcg tctggagccg caacggtaat ctggcctata
     8041 agatggggcg cggcatacag gctgggcgcg tgtggaccaa ctgttatcac gcttacccgg
     8101 cacatgctgc gtttggtggc tacaaacaat caggtatcgg tcgcgaaacc cacaagatga
     8161 tgctggagca ttaccagcaa accaagtgcc tgctggtgag ctactcggat aaaccgttgg
     8221 ggccgttctg atataagaag ctggtcgcat tgggtattca ttgcctgatg cgacgcttac
     8281 gcgtcttatc atacctacgg gaacctgacc gtaggccgga taaggcgtac acgccgcatc
     8341 cggcataaac aaagcgcacg ttgttacaat ttacgtttta aacctacgtg cgcaataacc
     8401 cagactacgc caacagaagt gcgtttctgg ggcgtgtttt gtcaggagtt taatctcgcg
     8461 tttcgatggt gagcgggcga accaggccgt aaatcaccat tggtgatccg aggccaatca
     8521 gcaataaacg gtagataatt gaaggtttaa tacgcttaat ctgtactgtg gatgttttac
     8581 tggagttatt cattagtttg gccaatagtt ccactatttc aatatgataa acctcgtcct
     8641 gttgaaaaac cagacagcac atttcagaaa gtatataaaa aacaatatgt cgttatataa
     8701 ataaggaaat tgcactgcat catggcattt cataatatgc gttaattgtt cttttttata
     8761 ttctttgagg gaaatatgtt cctggactat tttgcactgg gagtgcttat ttttgtattt
     8821 ctggtgatat tctatgggat tattatttta catgatattc cctacctgat ttctaaaaaa
     8881 cgtaatcatc ctcatgccga cgccattcat gttgctggtt gggtgagtct ttttacgctg
     8941 catgttatct ggccattttt gtggatttgg gccacgcttt accgcccgga gcggggatgg
     9001 ggaatgcaaa tccatgactc ctccgttgtg caactacaac agcgtattgc cgggctggag
     9061 aaacagctcg ccgacatcaa atcctcttct gccgagtaat acttatggat ttactgatta
     9121 ttttgaccta tgtggctttt gcatgggcaa tgtttaagat cttcaaaatt cctgtaaata
     9181 aatggaccat ttccacagcg gccctgggtg gcatatttat tgtcagtggt ttaattctgt
     9241 taatgaacta taaccatccg tataccttca aggcgcaaaa agcggttatt tctattcctg
     9301 ttgtcccaca ggtgacaggc gtggtgatcg aagtgacgga taagaaaaat acgctgatta
     9361 aaaaaggtga ggtgctattt cgactggacc cgacgcgtta tcaggcgcgg gtggatcggc
     9421 tgatggcgga tatcgttacc gcagaacata aacagcgggc gttgggcgca gagttagatg
     9481 agatggcggc gaatactcag caggcaaagg ccacgcggga taaattcgct aaagagtatc
     9541 agcgttacgc acgcggcagc caggcgaaag taaacccgtt ttcagaacgc gatattgatg
     9601 tggcgcggca aaattatctg gcgcaggaag cctccgtaaa gtcatcggcg gcggaacaaa
     9661 aacagatcca gagccagctg gatagcctgg tgttgggtga acattctcaa atcgccagcc
     9721 tgaaagcgca gctcgcggaa gcaaaatata accttgagca gacgatagtg cgtgcgccga
     9781 gcgatggtta tgtgacccag gtgctgattc gtccgggtac ctatgccgcg tcgctgccgc
     9841 tacgtccggt gatggtgttt atacccgatc agaaacgaca aattgtggcg cagttccgtc
     9901 agaactcctt gctgcgactg gcccctggcg acgatgcgga agtggtgttt aatgctctgc
     9961 caggtaaggt attcagcggc aagctggcag ccattagtcc agctgttccc ggcggagctt
    10021 atcagtcgac cggcacctta cagacgttaa acacagcgcc gggttcagat ggcgttatcg
    10081 cgaccattga actggatgag cacactgatt tgagcgcttt accagacggt atttacgccc
    10141 aggtggcggt ctactctgat catttcagcc atgtctcggt gatgcgcaaa gtgctattac
    10201 gcatgaccag ctgggtgcat tacctttatc tcgatcatta atcttcgctt tccgccgctt
    10261 gttcaggccg cagttgctgc gactgagcaa gctggtggcg aatttcacgc agcatttcat
    10321 ccagcacata ctggacccgc cagggcaggt attcccgttt acggaagata gccaccagat
    10381 ccagccacca ttcatacggg tgctgaaagc agggcaccag cgtgccgtct tgcagatcgc
    10441 tctgcacact tttgtctggc gcaaacacaa tccccaggtg atttcgtgcc agctccagcg
    10501 ccgattgcgt gttatcgcaa acgtaatttc ccttcacgcg ataatcccgc acctctttac
    10561 tcccggcgac gttgaagcgc cagatattcg cgtcgtcgat catcatcgag tcgatcaaaa
    10621 tacaggagtg atgttcgagt tcgtcaggac ggctaatagg atgtttctca agatagcgct
    10681 ggctggcata agcggttacg gcatattgtg taataaaact ggcaaccagc gattcatctt
    10741 ttggtggagc gtagctgatt aaaacatcac aatcatcagg aaattcgacg ccttcataaa
    10801 aggcattacg ctcaa
//
LOCUS       DAJTYF010000126        10665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTYF010000126 DAJTYF010000000
VERSION     DAJTYF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.126"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="OO590_003419"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="OO590_003419"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6638667.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="OO590_003420"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="OO590_003420"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR6638668.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="OO590_003421"
     CDS             3115..3399
                     /locus_tag="OO590_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6638669.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO590_003422"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO590_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6638670.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO590_003423"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO590_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6638671.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO590_003424"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO590_003424"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6638672.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="OO590_003425"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="OO590_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6638673.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9589..>10665
                     /gene="ompC"
                     /locus_tag="OO590_003426"
     CDS             9589..>10665
                     /gene="ompC"
                     /locus_tag="OO590_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6638674.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVA"
BASE COUNT         2618 a         2743 c         2655 g         2649 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacacttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc accgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atgggtgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaacggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaacaccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcacctcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc atacctcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatgc tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt atttcatcta tttttctcat
     9481 tgtttttaat caccattcta ttttaaaaac agtatgtcat ttataaatga cgccgtttaa
     9541 aattcgttta gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa
     9601 atagttgcgg tggttgtaac tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc
     9661 tataacaagg atggtaataa actcgacctt tatggcaagg ttaccgctct acgttatttt
     9721 actgatgata agcgtgacga tggtgataaa acttatgccc gtctcggctt taaaggagaa
     9781 acgcaaatca atgatcaaat gattggtttt ggtcactggg aatatgattt taaaggctat
     9841 aacgatgaag ccaacggctc gcgcggcaac aagacccgtc tggcctatgc tggtttaaaa
     9901 attagtgaat ttggctctct ggactatggt cgtaactacg gtgtcggcta cgacattggt
     9961 tcatggaccg atatgttgcc agaatttggt ggcgatacct ggagtcagaa agatgtcttc
    10021 atgacatacc gtaccaccgg tctagcaacc tatcgcaact acgatttctt tggcttaatt
    10081 gaagggctga actttgccgc gcaatatcaa ggcaaaaatg aacgtactga caacagtcat
    10141 ctttatggtg ctgactacac gcgtgccaac ggtgacggtt tcggtatctc ctcaacttat
    10201 gtttatgatg gctttggtat cggtgcggta tataccaaat ccgatcggac aaatgcgcag
    10261 gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga atgcagaact atgggccaca
    10321 ggtataaagt atgatgccaa caacatctac tttgcagcta attacgctga aacattaaac
    10381 atgaccacct atggggatgg ttatatctct aacaaagcac aaagttttga agtggtggcg
    10441 caatatcaat tcgacttcgg cttgcgccca tcactcgctt acctgaaatc gaaaggcaga
    10501 gatctgggcc gctacgccga ccaggacatg attgagtata tcgacgttgg tgcgacgtat
    10561 ttcttcaaca aaaatatgtc gacctatgtt gattataaaa tcaacctgat tgatgaaagc
    10621 gactttaccc gtgccgtaga tattcgcacc gataacatcg tcgct
//
LOCUS       DAJTYF010000127        10463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTYF010000127 DAJTYF010000000
VERSION     DAJTYF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.127"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(66..1070)
                     /gene="cra"
                     /locus_tag="OO590_003427"
     CDS             complement(66..1070)
                     /gene="cra"
                     /locus_tag="OO590_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6638675.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAEMVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1152..1238)
                     /locus_tag="OO590_003428"
     CDS             complement(1152..1238)
                     /locus_tag="OO590_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6638676.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO590_003429"
     CDS             complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO590_003429"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6638677.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO590_003430"
     CDS             complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO590_003430"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6638678.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3587..3631
                     /locus_tag="OO590_003431"
     CDS             3587..3631
                     /locus_tag="OO590_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638679.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO590_003432"
     CDS             complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO590_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6638680.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     EMEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGCDKGHQWMEEQLVSICKR"
     gene            5390..5476
                     /gene="leuL"
                     /locus_tag="OO590_003433"
     CDS             5390..5476
                     /gene="leuL"
                     /locus_tag="OO590_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6638681.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5569..7140
                     /gene="leuA"
                     /locus_tag="OO590_003434"
     CDS             5569..7140
                     /gene="leuA"
                     /locus_tag="OO590_003434"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6638682.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7140..8231
                     /gene="leuB"
                     /locus_tag="OO590_003435"
     CDS             7140..8231
                     /gene="leuB"
                     /locus_tag="OO590_003435"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6638683.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8234..9634
                     /gene="leuC"
                     /locus_tag="OO590_003436"
     CDS             8234..9634
                     /gene="leuC"
                     /locus_tag="OO590_003436"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6638684.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9645..10250
                     /gene="leuD"
                     /locus_tag="OO590_003437"
     CDS             9645..10250
                     /gene="leuD"
                     /locus_tag="OO590_003437"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6638685.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10299..>10463)
                     /locus_tag="OO590_003438"
     CDS             complement(10299..>10463)
                     /locus_tag="OO590_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6638686.1"
                     /translation="AATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVA
                     LFLTAKVKDV"
BASE COUNT         2736 a         2745 c         2562 g         2420 t
ORIGIN      
        1 gatgtccagt cccgtactct acgcgccaga gggaaattca cctggcgcgt atttttgttc
       61 gcggcttagc tacggctgag cacgccgcgg cgatagagat tacgtttaat gcgcgttaaa
      121 ccaggttttg gcttacgcgg ttcgtccagg cttgccagga caatctccag cacacgctct
      181 gcgacatcgc ggtgacgttg agccactgcc agcaccggac actgtaagaa gtcgagcagt
      241 tcgttatcgc caaaggtggc aattgccagg tcagaaggca gtttgccgtc gcgacgcagc
      301 gtgacatcca tcactccttg caacaacgca aacgacgttg tgaacagcgc ctgcggcatc
      361 ggatgcgttt ccagccattt ttcgaataac tgggcagccg cctcccgctc atagctgttg
      421 gcatacagga aatgcacttc gcgcggatca tctttccaag cagtacggaa accttgttca
      481 cgcaggaagc tgacagaaag ctccggcagc gcgccaagat aaagcaccat ctcggcggga
      541 aacttacgta actcttccgc cagcatttcg gcatcatcct gatcggcacc aaccacgctg
      601 gtgaaatgtt cacgatcgag ggcgcggtcc agcgcgacaa tcgggaacgg gtcgttagcc
      661 cagcgttgat aaaaaggatg ctcaggtggc aacgacgtcg aaacaataat ggcatcaacc
      721 tgacgctgta aaaggtgctc aatgcagcgc atttcgttgt ctggctgatc ttctgagcag
      781 gcaatcagca gttgataacc ccgttgccgc gcctggcgtt caagatagtt agcgatgcgg
      841 gtatagctgg tgttctccag atcggggatc acaagaccaa tagaacgtgt gcgtccagca
      901 cgaagcccag ctgccacggc gttcgggtgg taattgtgct cacgcaccac agccatgact
      961 ttttcaacgg ttttgtcgct cacacggtat tgcttcgctt tgccgttaat aacatagctt
     1021 gcagtggtcc gcgacactcc cgccagccga gcgatttcat ccagtttcac aattgcccct
     1081 tgcgtaaaat gtaaaaacca tagccattgt actggtatgg gttaaattca ttaacatcta
     1141 agcgcagaaa attaactgcg gcaactgctt ttattccagg ttcccactga tttcgcaaaa
     1201 aaagcccaac gtgacatgtt gggctgcaaa ttgcgcactg agatcattct caacgcatta
     1261 ttttatcgcc gcgcgaaagt ccgaccacac cagagcgagc aacttccaca attttcgcca
     1321 catcgcgaat cgatgctaaa aatgcatcaa gcttatcgct ggtgcctgct aattgaacgg
     1381 tataaagcga gggggtgaca tcgataattt gcccacggaa tatttccgta ttacgtttca
     1441 cttcgtcacg cccgtaaccg ctggcctgaa ttttcaccag catgatttcc cgctcaacat
     1501 gcgcgccctg ccccaactca ctcacgcgca agacatcgac cagcttgtgt aattgctttt
     1561 cgatctgctc aagtactttt tcatcgccca cggtctggat ggtcatacgc gataatgtcg
     1621 gatcgtcggt tggcgcaacg gtcaggcttt caatgttgta gcctcgctgg gaaaaaaggc
     1681 caatcacgcg ggataacgcg cctgattcat tttcgagtaa gactgataat atccggcgca
     1741 taatcaggtt ctctccgttt tgcttaacca catttcatcc attccgcccc cgcgaatctg
     1801 catcgggtag acgtgctcac tgccatcgac ggtaacatca acaaacacca ggcgattatt
     1861 gcgcacctgt tccagcgcct cgctaagttt gctttccagc tcatgcggat gagagatctg
     1921 gatcccgacg tgaccatagg cttccgccag acggacgaaa tcgggtagtg attgcatata
     1981 agattgtgaa tgacggccgg aatagatcat gtcctgccac tgtttcacca tccccagata
     2041 gcggttattg agattcacca ccagtacggg caactcgtat tgcaacgcgg tagacagttc
     2101 ctggatgttc atctgaatac tgccgtcgcc agtgacgcaa accacggttt cttctggcaa
     2161 cgccattttg acgcccagtg ccgcaggtaa accaaaaccc atcgtgccga gaccgccgga
     2221 attgatccag cgacgcggtt tgtcgaatgg ataataaagc gcagcaaaca tctggtgctg
     2281 cccgacatcg gacgtcacgt aagcgtcccc attagtcaac cgccaaagag tctcgatcac
     2341 cgcctgcggt ttaatctttt cactgtgagt gtcatatttc aggcactgaa gagcgcgcca
     2401 ctgttcaatc tgctgccacc agtcgcggat ctcatccagc ggttgatggg cggattcttg
     2461 cgacaggagt tcaagcattt gttcgaggac ctggcgagca tcccccacaa tcgggatatc
     2521 cgcagtcacg gttttagaaa tggaagtagg atcaatatcg atatgcagaa cagtggcatt
     2581 tgggcagtac tttgccagat tgttcgtcgt tcggtcatca aatcgtaccc cgacggcgaa
     2641 aatcacatcc gcgttatgca tcgtcatatt ggcttcgtag gtaccgtgca ttcccagcat
     2701 gcccagtgcc tgacgatgcg ttgccggaaa cgcccccagc cccatcaatg aggaaacaac
     2761 gggcagattc aacgcctcca ccgtttcttt caactgctga tggcagcccg ccatgattgc
     2821 cccaccgcct acgtagacaa ccggtttttt tgccgctacc agcgtttgca gagcacgctt
     2881 aatttgccct ttatgtccgg tagtagtggg attgtaagaa cgcatactga ccgactcagg
     2941 gcagacatag ggtaatttgt tcgccggatt aagaatatct ttcggtaaat caacgaccac
     3001 tggtcctggg cgaccacttg ccgccagcca gaaagccttt ttcagcacct gcggaatgtc
     3061 ttccgtttgc ttaaccagaa aactgtgttt aaccaccggt cgcgaaatcc ccaccatgtc
     3121 gcactcctga aaagcatcgt aacctatcaa cgaggtcgct acctgcccgg aaaggacaac
     3181 taatggaatg gaatccatat aagcggtggc gatgccggta atcgcattgg tcgcccctgg
     3241 acccgacgtt accagcacga cgccgacttc cccggtcgca cgcgccaggc catcggccat
     3301 atgcaccgcc gcctgctcat gacgaactaa tacatgatca ataccaccca cggtatgcaa
     3361 tgcatcgtaa atatcaagga ctgcgcctcc gggataaccg aatacttgtt taacgccctg
     3421 atcgataagc gatcggacga ccatctcggc tccagacaac atctccatgg cctgcctcac
     3481 tgtttgacgg aaaaaatgtg taaaacacat taaccgtccg atcttgctta agcaattggc
     3541 agccagagac ataaatgcag ggtaataaga ataaacagga ggaaaggtga aaagaaacga
     3601 taaaatcctc cattacgaag gattaaattg ataataaatc ggctgaatcc cacaacttac
     3661 agcaatttct ctattttttt aattaatcct aaaatagaga gtccacacat tcagaataaa
     3721 attctacatt caataaaaaa tgcagaataa accagacatt catgtctggc ctattctgca
     3781 ttcagttagc gtttgcaaat tgagactaat tgttcttcca tccactgatg gcctttatcg
     3841 cacccggcag cttcatgcca agagagataa caggttctgc tgttttgttt taacggcagc
     3901 ggtaatacct gtaattctaa ggattcagcg aactcttcag ccagccaacg cggcgcaata
     3961 gcgaccaaat gcgtttgcga caccacgcta agtacgctca tcattgccat gccctgatac
     4021 gcgatactgg cttgcttatc taccgtgtca taccaaggtt gactaaatga cgcgaaacga
     4081 tcgagcgaaa ccgccgcatg ttgttcgtta taaacatcat gtttcagtaa cgggccttta
     4141 attgttggat gatttttgct ggctaccagc accatttcat ctttaaataa tggcacgctg
     4201 gtaaattcag gacgatggaa gtcttcataa ctaatcacaa actccatttc ctgataacgc
     4261 agctgatgtt cagtgttctg atttaatgaa gacttgaaca taacatgtat atttggcgca
     4321 atctgctcaa tgtgattata aatctgcgag gtcagaatgc tgtctaacgg gctgcaaaca
     4381 caaagatgaa atacacgttc actgctcgcg ggttcaaaac ctgaaccagg caattcattt
     4441 tgtactagtt gcaatgcctg gcgaactgaa ccaaaaagtt gaaatgcgcg agcagtcggt
     4501 tgaataccac ggccataacg aacaaaaagc tcgtcattaa acatcacctt caggcgtgca
     4561 acagcgttac tgaccgcagg ttgcgacata cccagaacat gagcggcacg ggtaatgttt
     4621 tgctcctgca tcacggcatc gaaaacggtt aataagttga gatcgaccat gcgtagctgt
     4681 ggcttgctta actccgccgt ctctgaatga tctgtttgta cctctggcat acttaactcc
     4741 actgtcacac ttaactccct ttcccttatt ggaatgcaga gaaataatcc tgaagaatat
     4801 gatttatcat gcatataaaa taagaaaaag caaaatgagt aaaattcgga aaacataaag
     4861 atacgggttc tgataaaaac agaatccatt gccgtggaat gagtcattta ccacagcata
     4921 ataatccata atgtttttgt tgcgaaaaca atctaatcat aactaccgcg aatactcaat
     4981 catctacaaa atggattaaa tgtgcttatt taataattat tttatatatt taatgcatta
     5041 attcttaaca ttaattgatc aataatattc accaaatcaa tatcaaaaaa aatcgcaaaa
     5101 catataattc aatacaaatc attaggatag gttttgcaac gcgtgcattt tgtccccttt
     5161 ttcctcgttg attagatgca aaaatttatg ctgaaatatg tcaaccgatg aaaagcgtcg
     5221 gtagttaagc agaaattaat atcgcttact ttaaccaccg tagcacaatt agctaatttt
     5281 acggatgcag aactcacgct ggcgggacgt ttttattgcg tcagggttga catccgtttt
     5341 tgtatccagt aactctaaaa gcatatcgca ttcatctgga gctgatttaa tgactcacat
     5401 cgttcgcttt atcggtctac tactactaaa cgcatcttct ttgcgcggta gacgagtgag
     5461 cggcatccag cattaagcca gcacgcagtc aaacaaaaaa cccgcgccat tgcgcgggtt
     5521 tttttatgcc cgaagcgagg cgctctaaaa gagacaagga cccaaaccat gagccagcaa
     5581 gtcattattt tcgataccac attgcgcgac ggtgaacagg cgttacaggc aagcttgagt
     5641 gtgaaagaaa aactgcaaat tgcgctggcc cttgagcgta tgggtgttga cgtgatggaa
     5701 gtcggtttcc ccgtctcttc gccgggcgat tttgaatcgg tgcaaaccat cgcccgccag
     5761 gttaaaaaca gccgcgtatg tgcgttagct cgctgcgtgg aaaaagatat cgacgtggcg
     5821 gccgaatccc tgaaagtcgc cgaagccttc cgtattcata cctttattgc tacctcccca
     5881 atgcacatcg ccaccaagct gcgcagcacc ctggacgagg tgatcgaacg cgctatctat
     5941 atggtgaaac gcgcccgtaa ttacaccgat gatgttgaat tttcttgcga agatgccggg
     6001 cgtacaccca ttgccgatct ggcgcgagtg gtcgaagcgg cgattaatgc cggtgccacc
     6061 accatcaaca ttccggacac cgtgggctac accatgccgt ttgagttcgc cggaatcatc
     6121 agcggcctgt atgaacgcgt acctaacatc gacaaagcca ttatttccgt acatacccac
     6181 gacgatttag gcctggcggt cggcaactca ctggcggcgg tacatgccgg tgcacgccag
     6241 gtggaaggtg caatgaacgg gatcggcgag cgtgccggta actgttcgct ggaagaagtc
     6301 atcatggcga ttaaagttcg taaggatatt ctcaacgtcc acaccgccat taatcaccag
     6361 gagatatggc gcaccagcca gttagttagc cagatttgta atatgccgat cccggcaaac
     6421 aaagccattg ttggcagcgg cgcattcgct cactcctccg gtatccacca ggatggcgtg
     6481 ctgaaaaacc gcgaaaacta cgaaatcatg acaccagaat ctattggtct gaaccaaatc
     6541 cagctgaatc tgacctctcg ttcggggcgt gcggcggtga aacatcgcat ggatgagatg
     6601 gggtataaag aaagtgaata taatttagac aatttgtacg atgctttcct gaagctggcg
     6661 gacaaaaaag gtcaggtgtt tgattacgat ctggaggcgc tggccttcat cggtaagcag
     6721 caagaagagc cggagcattt ccgtctggat tacttcagcg tgcagtctgg ctctaacgat
     6781 atcgccaccg ccgccgtcaa actggcctgt ggcgaagaag tcaaagcaga agccgccaac
     6841 ggtaacggtc cggtcgatgc cgtctatcag gcaattaacc gcatcactga ctataacgtc
     6901 gaactggtga aatacagcct gaccgccaaa ggtcacggta aagatgcgct gggtcaggtg
     6961 gatattgtcg ccaactacaa cggtcgccgc ttccacggcg tcggcctggc caccgatatt
     7021 gtcgagtcct ccgccaaagc catggtgcac gtactgaaca atatctggcg tgccgcagaa
     7081 gtcgaaaaag agttgcaacg caaagctcaa cacaacgaaa acaacaagga aaccgtgtga
     7141 tgtcgaagaa ttaccatatt gccgtattgc cgggagacgg tattggtccg gaagtgatga
     7201 cccaggcgct gaaagtgctg aatgccgtgc gcaaccgctt tgcgatgcgc atcaccacca
     7261 gccattacga tgtaggcggc gcagccattg ataaccacgg gcaaccactg ccgcctgcga
     7321 cggttgaagg ttgtgagcaa gccgatgccg tgctgtttgg ctcggtaggc ggcccgaagt
     7381 gggaacattt accaccagac cagcaaccag aacgcggcgc gctgctgccg ctgcgtaagc
     7441 acttcaaatt attcagcaac ctgcgcccgg caaaactgta tcaggggctg gaagcattct
     7501 gtccgctgcg tgcagacatt gccgctaacg gcttcgacat cctgtgcgtg cgcgaactga
     7561 ccggcggcat ctatttcggt cagccaaaag gccgcgaagg tagcggacaa tatgaaaaag
     7621 cgtttgatac cgaggtgtat caccgttttg agatcgagcg tattgcccgc atcgcgtttg
     7681 aatctgctcg taaacgtcgc cacaaagtca cctcaatcga caaagctaac gtgctgcaat
     7741 cctctatttt atggcgtgaa atcgtcaacg agatcgccac ggaatacccg gatgtcgaac
     7801 tggcgcatat gtacatcgac aacgccacca tgcagctgat taaagatcca tcacagtttg
     7861 acgttctgct gtgctccaac ctgtttggcg acattctgtc tgacgagtgc gcaatgatca
     7921 ctggctcgat gggcatgttg ccttccgcca gcctgaacga gcaaggtttt ggactgtatg
     7981 aaccggcggg cggctcggca ccagatatcg caggcaaaaa catcgccaac ccgattgcac
     8041 aaatcctgtc gctggcactg ctgctgcgtt acagcctgga tgccgatgat gcggcttccg
     8101 ccattgaacg cgccattaac cgtgcattag aagaaggcat tcgcaccggg gatttagccc
     8161 gtggcgctgc cgccgttagt accgatgaaa tgggcgatat cattgcccgc tatgtggcag
     8221 aaggggtgta atcatggcta aaacgttata cgaaaaatta ttcgacgctc acgtagtgta
     8281 cgaagccgaa aacgaaaccc cgctgttata tatcgaccgc cacctggtgc atgaagtgac
     8341 ctcaccgcag gcgttcgatg gtctgcgcgc ccacggtcgc ccggtacgtc agccgggcaa
     8401 aaccttcgcc actatggatc acaacgtctc tacccagacc aaagacatta atgcctgtgg
     8461 tgaaatggcg cgcatccaga tgcaggagct gattaaaaac tgcaaagaat ttggcgtcga
     8521 gctgtatgac ctgaatcacc cgtatcaggg gatcgtccac gtaatggggc cggaacaggg
     8581 cgtcaccttg ccggggatga ccattgtctg cggcgactcg cataccgcca cccacggcgc
     8641 gtttggcgca ctggcctttg gtatcggcac ttccgaagtt gaacacgtac tggcaacaca
     8701 aaccctgaaa cagggccgtg cgaagaccat gaaaattgaa gtccagggca aagccgcgcc
     8761 gggcattacc gcaaaagata tcgtgctggc aattatcggt aaaactggca gcgcaggcgg
     8821 caccgggcat gtggtggagt tttgcggcga agcaattcgt gatttaagca tggaaggtcg
     8881 tatgaccctg tgcaatatgg cgatcgaaat gggcgcaaaa gccggtctgg ttgctcctga
     8941 cgaaaccacc tttaactatg tcaaaggccg tctgcatgcg ccgaaaggca aagatttcga
     9001 cgacgccgtt gcctactgga aaaccctgca aaccgacgaa ggcgcaactt tcgataccgt
     9061 tgtcactctg caagcagaag aaatttcacc gcaggtcacc tggggcacca atcccggcca
     9121 ggtgatttcc gtgaacgaca atattcccga tccggcttcg tttgccgatc cggttgaacg
     9181 tgcgtcggca gaaaaagcgc tggcctatat ggggctgaaa ccgggtattc cgctgaccga
     9241 agtggctatc gacaaagtgt ttatcggttc ctgtaccaac tcgcgcattg aagatttacg
     9301 cgcggcagcg gagatcgcca aagggcgaaa agtcgcgcca ggcgtgcagg cactggtggt
     9361 tcccggctct ggcccggtaa aagcccaggc ggaagcggaa ggtctggata aaatatttat
     9421 tgaagccggt tttgaatggc gcttgcctgg ctgctcaatg tgtctggcga tgaacaacga
     9481 ccgtctgaat ccgggcgaac gttgtgcatc caccagcaac cgtaactttg aaggccgcca
     9541 ggggcgcggc gggcgcacgc atctggtcag cccggcaatg gctgccgctg ctgctgtgac
     9601 cggacatttc gccgacattc gcaacattaa ataaggagca caccatggca gagaaattta
     9661 tcaaacacac aggcctggtg gttccgctgg atgccgccaa tgtcgatacc gatgcaatca
     9721 tcccgaaaca gtttttgcag aaagtgaccc gtacgggttt tggcgcgcat ctgtttaacg
     9781 actggcgttt tctcgatgaa aaaggccaac agccaaaccc ggacttcgtg ctgaacttcc
     9841 cgcagtatca gggcgcttcc attttgctgg cacgagaaaa cttcggctgt ggctcctcgc
     9901 gtgagcacgc gccctgggca ttgaccgact acggttttaa agtggtgatt gcgccgagtt
     9961 ttgctgacat cttctacggc aatagcttta acaaccagct gctgccggtg aaattaagcg
    10021 atgcagaagt ggacgaactg tttgcgctgg tgaaagccaa tccggggatc catttcgacg
    10081 tggatctgga agcgcaagag gtgaaagcgg gagagaaaac ctatcgcttt accatcgatg
    10141 ccttccgccg ccactgcatg atgaatggtc tggacagcat cggcctgacc ctgcagcacg
    10201 acgacgcgat tgcgtcttat gaagcaaaac aacctgcgtt tatgaattag accccttgcc
    10261 cggtcaaatg accgggcttt ccgctatcgt ccacgtcatc aaacgtcttt aacctttgcg
    10321 gttaaaaata atgcgacaac agaaataatc gcaattaccc agtagacagc aaagtgcccc
    10381 caactttgtg caattgctcc ctgaataaca ccagccagaa ttaccccggt agagatagtg
    10441 ttggtaaata aggtggtagc tgc
//
LOCUS       DAJTYF010000128        10248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTYF010000128 DAJTYF010000000
VERSION     DAJTYF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.128"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            358..1074
                     /locus_tag="OO590_003439"
     CDS             358..1074
                     /locus_tag="OO590_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6638687.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLDAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1530..1605)
                     /locus_tag="OO590_003440"
     tRNA            complement(1530..1605)
                     /locus_tag="OO590_003440"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1570..1572),aa:Asn,seq:gtt)
     gene            complement(1706..3333)
                     /gene="yeeO"
                     /locus_tag="OO590_003441"
                     /pseudo
     CDS             complement(1706..3333)
                     /gene="yeeO"
                     /locus_tag="OO590_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3338..3413
                     /locus_tag="OO590_003442"
     tRNA            3338..3413
                     /locus_tag="OO590_003442"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3371..3373,aa:Asn,seq:gtt)
     gene            complement(3451..4401)
                     /gene="cbl"
                     /locus_tag="OO590_003443"
     CDS             complement(3451..4401)
                     /gene="cbl"
                     /locus_tag="OO590_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6638688.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4503..5420)
                     /gene="nac"
                     /locus_tag="OO590_003444"
     CDS             complement(4503..5420)
                     /gene="nac"
                     /locus_tag="OO590_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6638689.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            5747..5822
                     /locus_tag="OO590_003445"
     tRNA            5747..5822
                     /locus_tag="OO590_003445"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5780..5782,aa:Asn,seq:gtt)
     gene            complement(5878..6813)
                     /gene="ldtA"
                     /locus_tag="OO590_003446"
     CDS             complement(5878..6813)
                     /gene="ldtA"
                     /locus_tag="OO590_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6638690.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(6875..7954)
                     /gene="cobT"
                     /locus_tag="OO590_003447"
     CDS             complement(6875..7954)
                     /gene="cobT"
                     /locus_tag="OO590_003447"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6638691.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(7966..8698)
                     /gene="cobS"
                     /locus_tag="OO590_003448"
                     /pseudo
     CDS             complement(7966..8698)
                     /gene="cobS"
                     /locus_tag="OO590_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
     gene            complement(8695..9240)
                     /gene="cobU"
                     /locus_tag="OO590_003449"
     CDS             complement(8695..9240)
                     /gene="cobU"
                     /locus_tag="OO590_003449"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6638692.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(9867..10130)
                     /locus_tag="OO590_003450"
                     /pseudo
     CDS             complement(9867..10130)
                     /locus_tag="OO590_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2918 a         2593 c         2171 g         2566 t
ORIGIN      
        1 ccgccgttcc gataataagt tatgaacctt cttatcagaa atagatgtga aggattaagt
       61 aagacatctg gcaaatagcc tgcaataaac gcgggctgct tgcttccttg ggcagatgcg
      121 agttttatta tcgtcttaat gatttccaca tattaaaagc aagtatgctt tcaaaacaca
      181 attataaaaa atcccgccaa caacattagt ttttataaaa ttaaatataa gattatggct
      241 ttagaatatt tttatttcta atagacgaga tttttcctgt tatgatataa tatgccgaat
      301 taacacatgt taacgattta ccagtaatgt aaataaattt tcgaggagat cattccagtg
      361 ggacgtaaat gggccaatat tgttgctaaa aaaacggcta aagacggtgc aacgtctaaa
      421 atttatgcaa aattcggtgt agaaatctat gctgctgcca aacaaggtga acccgatcca
      481 gaattaaaca catctttaaa attcgttatt gaacgtgcaa agcaggcaca agttccaaag
      541 cacgttattg ataaagcaat tgataaagcc aaaggcggcg gagatgaaac gttcgtacag
      601 ggacgttatg aaggctttgg tcctaatggc tcaatgatta ttgctgagac attgacttca
      661 aatgttaacc gtacgattgc taacgttcgc acaattttca ataaaaaagg cggcaatatc
      721 ggagcggcag gttctgtcag ctatatgttt gacaatacgg gtgtgattgt atttaaagga
      781 acagaccctg accatatttt tgaaattttg cttgacgctg aagttgatgt tcgtgatgta
      841 actgaagaag aaggtaatat tgttatttat actgaacctg ctgaccttca taaaggaatc
      901 gcggcgctaa aagcagctgg aatcactgag ttctcaacaa cagaattaga aatgattgct
      961 caatctgaag ttgagctttc cccagaagat ttagaaatct ttgaagggct tgttgatgcc
     1021 cttgaagatg acgacgatgt acaaaaagtt tatcataacg tcgcaaatct ctaattatat
     1081 tttaaagaaa tctgtcttta tggcagattt ctttaatctc atataattct tataaaaaat
     1141 ataatgttta actcgtcata ttgcttataa aaaatccccg ttcccagaga aataatattt
     1201 attaaaatgg caggtcttct ttttctgatt accgaagaca aagtggcaat tacgtatagt
     1261 ttcccgataa agacgcgaca gctacatccc gcataaggca tttttctctt tatctttgta
     1321 cggtacttca tggaacagaa tttttgacct tgtgaatcgt gatgtctgtt ggggaggggc
     1381 aatttgctca ctgaagcgtg agactcgatt aagcgcacga aacacagaaa tcaaaaaacc
     1441 cggccacttt tttacaaagt ggccgggtaa aaataatttt tattttttaa ctgtcctgag
     1501 tctcatagag atgtctcaaa actaaaattt ggctcctctg actggactcg aaccagtgac
     1561 atacggatta acagtccgcc gttctaccga ctgaactaca gaggaatcgt gagaacgagg
     1621 cgaatattag cgatgcccac ccacaatgtc aaagcctgtt ttttaaattt gaaatcgttt
     1681 gctgaaataa tctgcatttt gtcgtttatt ccgacacaac tggctttttt tcacactttt
     1741 gcggctcgga tcgagggtat ttccacagcc aacgtccagt aaccattcgc cagtaaaaca
     1801 gcacggcccg cacagcccag taggcaaaca ttcccatcca gacaccaacc acaccccagc
     1861 caagcatgat tccctgcaca taaccgacta caacccggca accccacatg ctcaacatag
     1921 aaacccacat ggcgtaacgg gcatcacgag cacccttaaa tccagcgggt agcacccatg
     1981 aggcggacca aataggcata aataaagcat ttagctaaat cagaatcaca acgacgtgtt
     2041 taacctgtgg atcctgggtg taaaacgatg ccataacccc ggcaaaggga gccgttagcc
     2101 aggcgatggc cgttaatcca agagtggaca gccagaacac atgccgcaac tgaatctctg
     2161 cttgcgctat ctgccctacc cccaaccttc ggcctgtaat gatcgtagaa gcagaaccga
     2221 gcgcacttcc gggtaagttg ataagagccg caattgagaa cgcgataaaa tttccggcaa
     2281 taacgctggt ccccatcccg gcaacgaaca tttgggttaa taaccgacca ctggtaaata
     2341 acactgattc gacactcgct ggaataccaa tccccatgac ttcccagata atgctaaaat
     2401 tcagcggttt aaaatagctc tttaacgaaa tccttagcgc aggattaaaa ccaatcgcca
     2461 gcacccacaa aattgcaact gcgccaatat aacgagaaat ggttaaaccc aacccagccc
     2521 cgaccaggag aaaaggccgt aaatcaatat gccgctaata ataatattaa gaatattcag
     2581 gctaccgtta atcagtagcg gtattttcgt attccctgca ccacgaagtg ccccgctacc
     2641 aataagagtg atggcagcgg ctggataact gagtacggtc agctccagat aagtcaacgc
     2701 cagtgcttta acttctgtcg tggcatcacc cgcgacgaaa tcaataattt gttcgccaaa
     2761 atgatgaatt agcgttgcca acagtacggc aaacaacgtc atgatcacca atgactgccg
     2821 cgtcgccacc ctcgctcgtc gtcgatcccg cttaccgaga ctaaatgcta caacgacagt
     2881 agtaccaaga tcgatagctg caaaaaaagc cataatgacc atattgaagc tgtccgccaa
     2941 ccccacgccg gccatcgcat cttttcccag ccagctgacc agaaaagtgc tcaggccccc
     3001 catcaacagg acacagacgt tctccatgaa gataggaaca gcaagcgggg ttatctcgcg
     3061 ccagaacaac actttgtagc tcttgcgttt agagtgccag cgagtgccgt gaacaacctg
     3121 gcgtaaagca gaggagatat tcaaagccga ccttaattgc agaaagtgaa accacatttc
     3181 aaatgatgag ggagaatcag caaagctgca aagattttcg ccaacaaatt gtctgcaaat
     3241 gcaacaaact gttgatagaa acggcaaaca gttgggaaat ttaaaaatcg ggtttgacaa
     3301 aatatttttc gccgttaaga tgtgcctcaa cagcgattcc tctgtagttc agtcggtaga
     3361 acggcggact gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaatttct
     3421 gaaaagcccg cttttatagc gggctttttg ctatatctga taatcaattt cctcttcact
     3481 gttttccatc acctggcgct tgatatcctc aactgacagt cctgcattac aaagttccag
     3541 aaagcgccaa acatagttac gctgaagttg tcctcgcttc agtcccaacc agacagtatt
     3601 agcatcgaaa agatgccgcg tatccaggcg gattaaattc tcttcctctt gttcgccgct
     3661 ggattgttcg gcaactaatc cgatcccaag cccaagagca acataggttt taatgacatc
     3721 agaatcctgt gcacttaata caatatctgc cggcaaacct ttgcgggtaa atgcgtcatc
     3781 agtgcgtgag cgccccgtaa ttccctgtcg gtaagtaatt aacggccact tcgctattga
     3841 ttccagcgtc aatggtgtaa tttgcgttaa gggatgatcg agtggaacaa gcaaactatg
     3901 gtgccaacaa aaccacggga aggcgacgag ctgcgggtca ttactcaaac gctcgctggc
     3961 gataccaata tcagcttcgc cattttgcaa caacgtcgca atttcctgtg gcgaaccctg
     4021 gattagctcg agccgaacct ccgggaaaag ttcgcgaaaa gctttaatga cctctggcaa
     4081 gctataacgt gcctgagtat gcgtcgttgc aatagtgaga acaccagacg tatcgttggt
     4141 aaacaggtct gcaagccgac gaacattact ggcttcattc agaatacgtt ctgcaatgac
     4201 cagtaatgct ttgcccggtt cagtcatccc cagcagtcgc ttacctcgtc gaacaaatat
     4261 ttcgatgcca agttcatcct ccagttcccg aatatgacgg ctaacgcctg actgtgaggt
     4321 aaaaagcata ttcgcaacct ctgtcaggtt gtaatcctga cgtgcagcct cgcggattat
     4381 ctttagttgt tggaaattca cggtaaactc cgggcagttc agatatcccg ttattgttaa
     4441 agtctaatgc ccggcataac aaataataaa aacccgcatc ttattccatc ccgatataac
     4501 gcttagctca ccaattgcca ctgccttttt tccatcactg gagaactaat cactgacatt
     4561 aacaactctt tcactgcctg tgcctgtggt gacaagtttg ctctggcggg taaatttaag
     4621 gacaaagaga gactcattga aggagtggta atgcgtgaca tccacccatt tactgcgcca
     4681 cataacgaac gcgcagccga ttcaggtaat actgcaacgc ccatgccgct ggcaatcgct
     4741 gcggtaagcg tggcaataga ctcaatttca ccaatgactt ttgccgtgag ccgccgtagg
     4801 gaaaaagcct catcaacacg aagtctaaca gcgctgtaat cactggggag aaaaaggttc
     4861 atttgcgcaa tagcattaac atcaacgttt tgccccgggc aatcttgagt tcctaccagg
     4921 aaaagatctt ctttcagcaa agcctgactg gataccccag ccacagggga atgctcataa
     4981 atcaccgcca tatcgagttg gtgatttatc agtttttcgt taagcactgc gccactattt
     5041 tcatgaagat agataacgac ctccgggaat tcagcgcgaa ccgcctgtaa taagggcatg
     5101 gtgatggatg acgcagcggt tcctggcgca aagccaatcg agacttgccc cgataatgac
     5161 tgaccaacgt tatgcaccgc cagttgggcc tgctcacact gacgtaaaat ggcccgtgca
     5221 tgggtataga gaatttttcc ggcgtctgtt ggcgtaacgc cccgttttgt gcgaatcaaa
     5281 agctgttgat ttaactcacc ttccagtatg gcaacctgct ggctgagcgc tggttgtgcg
     5341 atatgcaata cttcagcagc ctgggtcagg ctaccaatat ctacaatttt tacgaagtat
     5401 ttcaggcgtc tgaagttcat gttgcctccg gtttttaaga atcggcccaa gtgccgccat
     5461 tacttacaac cagtatgcaa gatgcctgcc agttctattt tggcgttgat gcgcaagcta
     5521 accaactgtc aaataagaga ttatgataga ttcgtcattg gcccttttta tcagcagtcg
     5581 cattcacctg ccctataaaa ggagggtatg caccacgatg gttcattacc caataagatt
     5641 gaaagctcac cactttgttg aaattggcag caaacaaaca aaaaaatgca tttcaccctt
     5701 tgacatcacc atgcactgcc attaatatgc gccccgttca cacgattcct ctgtagttca
     5761 gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc
     5821 caaattcaaa aaagcctgct ttcgagcagg ctttttactt tctaattacc aacgctctta
     5881 aaacatctgt cttgaaccag aactaatttg cacaggcatt cccgatcgat gttgcaacgc
     5941 agcatttgcg cgatttacat caacttcttg cccgttgata aacgcccgca aagatggggt
     6001 taccggcaat ggcacttttc ggtcagactc atattctgca cgattgcgtg acagcggctc
     6061 atgaacttcc agccactttg aaccatccgg ttccgttgtg tatttcactg gctgatcaat
     6121 aatttgtaca cgtgtgccta caggaacatt atcaaacaga tatttgatat cgtcattacg
     6181 cagacgaata cagccctgac ttacacggag cccaatacca aaattggcat tggtaccgtg
     6241 gatggcatac aacctgccaa tataaatcgc gtacagcccc atggggttat cgggccccgc
     6301 aggaacaaat gcgggcaaac tctcccctcg tttcgcatat tcgcgccggg tgttcggcgt
     6361 tggcgtccag gttggtgctt cttgtttacg ttcaacggta gtcacccagt tacgcggggt
     6421 ttctcgccca gcctggccga taccaatagg aaagacttcc actgtattac tgtctggcgg
     6481 atagtaataa agacgcattt cagcgacgtt aaccacaatc cctttacgaa cagtggcggg
     6541 caaaatcagt tgctgcggaa tggtgagttg tgagccaggc ttcggtaaaa aaacatcagc
     6601 gcccggattc gcttccagca tattacttaa cccttgcccg tattgtgcgg caaaagtctc
     6661 cagcggctga gtattgtgat caggaacagt tacagtaagc gactgcccca ctaaacggct
     6721 accctctgga ggtaatggat aagttaccgc caggctattt tggctggcaa aaagcagagc
     6781 aaatgagcaa agaatattta cacgacgcat catgtccctt tcctaagtcg cgaatactat
     6841 ccgataagta tagcttttat cagacttttc gtttttagct gttcaaatca gaagtcgtat
     6901 tccccggtag aacaatatta ctggcagcaa gttcgcccat gttgttgtat atcgcacaag
     6961 cagcttcgat gatggacatc gccagagctg caccactccc ctcacctaaa cgcatatcca
     7021 tattgagata aggctccagc cccaaatgcg agagcgctat acgcgcgcct ttttctgccg
     7081 acaagtgaga aggaatgaga tacggtttga ttgcaggaga catctggcag gctgcgagcg
     7141 cagcagcata agaaagaaat ccatccagca acacgggtaa accgcaggaa gcagcaccta
     7201 acatcactcc agctattccg accaaatcaa atccacccac ttttgccagg acatcaacac
     7261 catcctgagg atttggttga ttcaacgtaa tcgcccgacg cacaacatca attttattag
     7321 ccagtttatc tgtcggcagg tttgcgccaa tcccaaccac ttcttcagga tcccggccag
     7381 tgattgtgct gactattgcc gctgccggtg tcgtgtttgc catccccagt tcacctacac
     7441 caaacagcgt gacaccgttt ttcgccagct cccgcgtata acatatgacg tccaaaagca
     7501 acttttcagc ctgacggcga ctcattgccg gagctgaagc aatattgccg ctacctcgtg
     7561 cgacacgcat gttgataagc ccggggatag gctcagcagt atcaatacca acatcaatta
     7621 cgtggacgtt agcgcccgct tgtgctgcca gcacacacac gccggttgtt ccacgggtca
     7681 tattttcagc ctgtatggct gtcacttctt ttggagaaat agcgacccct tcctcccaga
     7741 cgccgtgatc ggcacacata accagtaccg cttttttgcc cacatgcggt atgccattca
     7801 cccccggcat tcctgccagt tgtatggcaa gcgcctccag ctttcctagg ctaccaacag
     7861 gtttgagtaa cccgtcaaca tgccgttgtg cacgcgacat agcggcagaa ttgatggctg
     7921 ggatcgtatt cagtaaatcg gcaattattt gcatgtcatg tctctttata gcagtgccag
     7981 caggaagacc agttcaccaa gctctatggc tgctcccagc gtatcgcccg tttgcccgcc
     8041 cagcgtacgt ttgagaagct gaccgaggat gaaaatcgcc accatcgtca ccaccatagc
     8101 agccacacca tgcataccca gcaataatac ggcagcaaaa atagcggcta agccgagtgt
     8161 gacgcaggtt tgtcgcgcca atgaacacat tgccaagccc ttcttcccgc gcataacgat
     8221 gacgatacat caataatgcg gcagtcccgc ggctgaccgc acatgctgcc gccagcgatg
     8281 caaggatcgg ttcgccacgc agagccaact cgcttaacac cagaatcttt gccagtacca
     8341 caaaaatcaa tgccagaccg ccgtgggtgc ctaaacggct atcacgcatg atttccaaca
     8401 tgcgatcgcg gctacgtgca gaaaatacgc catcacaggt atcagcgagg ccgtccagat
     8461 ggaattcccc ggtcatcagc gccagcacaa gtacgctaaa cagtgccgcc agtggtgcgc
     8521 cacaccatgc ctgcagcacc atgaagacca gcccgctaat cgcgccaagc aataatccaa
     8581 tcaaaggaaa agtaataata ccgcgagaat aatgctcgaa atccagtccc tgggaccagc
     8641 gacgcggtac aggcagacgc gtaataaatg agagcatcgc ccaaaataat ttgctcattt
     8701 gatttttact ccaatacccg aaaccaccag ccatacttca tttgccgcag cggccaactg
     8761 ctgatttacc cgcccggcaa tatcacgaaa atgtcgtgcc agacgactct ccggcacaat
     8821 ccccattccc acttcgttag tcactaatac aacctttgcg gggcaacgtt ggcaggcagc
     8881 aatcaacgac tgaatctcag cattaatcgc ctgttccatc gcctgataat cccattcatc
     8941 agggtcttta tcgccgccat aatcaaacaa cagattagtc accattgtgg taacgcattc
     9001 cagcaacaca gcctcattcg ggttaatgtc tgcatgaatt aattcatcaa gatgttgcca
     9061 gcgctccact gtgcgccagt gctccgggcg gctttgccga tgatgttcta tccgtgcagc
     9121 catctcatca tcaaggattt gcgaggtagc gatatacaga acctgtgaag agtccccaat
     9181 aagcacctct gcatggcgac tcttcccgct ccatgcgcct cccgtcacca aaatcatcat
     9241 accgactcct gattcaaatg tatgatggta tacattgaac agagcaatgg atcccccacc
     9301 gttgctgacc gcaaaacatt tacgcgatta atactgcgcg taatataatt aaaaatccag
     9361 cattctcaat ccattacgaa ataagatggc attacggaat agccagactt tctcgtgtta
     9421 acgtccgaca aaatgcgtca ttgacagcgg tttctgtgga ttgacacatt tcattcagat
     9481 aatgaattaa tgctactgcc ggaccaacca gcaaacgggc attgtgttct gaaatccagg
     9541 ctattgattc aacgtcagcg acagatctgc gctgtaaaag aacttgtaaa acaacgcaat
     9601 agaattatcc ggaatggtgg cgactatgca ctagaacacc acaatttcgc tctctcggta
     9661 aggcactgtc acagcattca acagaatgtg acttgccaga tttattagcg ccaccagatg
     9721 tttaaccggg taaccacgac ccagtcgggg atatattcca tcttctcgct gacatcatca
     9781 agaataaaaa ggtttatcac actcagagca acgttcttca gcaggcggga taattcttca
     9841 acatttaaaa gatgtgttat taaaggcatc tcaaattgga cagggctgcc ggttttctgt
     9901 tgcaacaccg ttgctctgct tgagacagaa ctgccatatg cagcatctga tactttgagt
     9961 ttgaccaaat cacagccccg tagcttactg tccaaagcca tgttgaacag agctagatcg
    10021 cgtgttttac cttccaactc aagtctaatc cggatccccc agatatgaga tatctgaagt
    10081 ggcctttttt gaccgatggt acggtcttta ttccacggtg acgttttcat attcaaatct
    10141 cctgcaatgt gggagatttg agtatggttg tctctcaaga gcgaaaataa gtcaactatc
    10201 actagttgtt gcgcatgtac gtattctgtc aattaattat tgacaatc
//
LOCUS       DAJTYF010000129        10079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTYF010000129 DAJTYF010000000
VERSION     DAJTYF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.129"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(122..394)
                     /locus_tag="OO590_003451"
                     /pseudo
     CDS             complement(122..394)
                     /locus_tag="OO590_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            complement(488..1050)
                     /locus_tag="OO590_003452"
                     /pseudo
     CDS             complement(488..1050)
                     /locus_tag="OO590_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1254..1460)
                     /locus_tag="OO590_003453"
     CDS             complement(1254..1460)
                     /locus_tag="OO590_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR6638693.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1567..2130)
                     /locus_tag="OO590_003454"
     CDS             complement(1567..2130)
                     /locus_tag="OO590_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6638694.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3505..5340)
                     /locus_tag="OO590_003455"
     CDS             complement(3505..5340)
                     /locus_tag="OO590_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638695.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5441..5728
                     /locus_tag="OO590_003456"
     CDS             5441..5728
                     /locus_tag="OO590_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6638696.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5700..7229
                     /locus_tag="OO590_003457"
     CDS             5700..7229
                     /locus_tag="OO590_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR6638697.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7399..8616)
                     /locus_tag="OO590_003458"
     CDS             complement(7399..8616)
                     /locus_tag="OO590_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6638698.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8983..9969)
                     /locus_tag="OO590_003459"
     CDS             complement(8983..9969)
                     /locus_tag="OO590_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638699.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9966..>10079)
                     /locus_tag="OO590_003460"
     CDS             complement(9966..>10079)
                     /locus_tag="OO590_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6638700.1"
                     /translation="EISNKPYLGNMLNDFAGVDQQRVMHCRKAFSDAKVFK"
BASE COUNT         3174 a         2295 c         1876 g         2734 t
ORIGIN      
        1 aaggctgggc catactcctc cggcagaagc agaaaaagct tattatgctt ccatcggaaa
       61 cgatgatctg gcagcctgag ttcacagata aaacactctc caggaaaccc ggggcggttc
      121 acttacacca gacatcgtta ttaagtagca gcaccataag ataaggagtg gcatcgttag
      181 ccacagcttc cctactccag aggtaatata aaggggtggg ctcgactgat ttatctttac
      241 gtcgcttaca ctgcaaatgt tcagaaatta gtctatgcag ctcaccagta aaatcagcaa
      301 tcagagaagg aattgcctta ttcataccag ggcaaggtat taatttaaac tgtaataatt
      361 taattttagg atgtgtagca gcagcccgat acagagttgc aaggacacac ttttgccaga
      421 gggcgttaca ggaaagctta atgtttgatt ctgtatacat aataaatcac cttatagtta
      481 caataggtca aaaaccgctg tagccaaagt tacgctggcc tgatgcttta gtgtcgagct
      541 tcgtcaaata atccagacgt accaataagc gctggtactg ctctgggaaa ttaggatcgt
      601 taatatccag gatgtaacgt ccattagcag ggaaatgaac taatccctgg tgatcttcca
      661 gttccaaccc taaggcactg taccaggctc cggtaattaa ccctctcagg gtgtcagagc
      721 gctcatagtt tcccagatgg taataggcgt ctttgttaaa aaaaggcaga cgtgataatg
      781 acatttaaag gactgcgtat attctttcgc ccagataata caaaccgacg ttgcgtaggt
      841 gcgtttgcct tgttgttttt tacggtgttg atctgcatta attttcgcat tcagcgagtt
      901 acggaaacga gatatctctc cggggatcag gttagggaaa caggtaatga tatccccgtt
      961 gtcaatcaca ggagggtaat ggagatcccc ccgtattgct gttattctgg aagtgttttt
     1021 aatattcgta ttgatagttc tcagagatat atcacactct agatatttta attaagtatc
     1081 agttcgatat cggcatattg tacactacct caggtagatg atgagatcag atacagggat
     1141 caactgacgg tgagcgcagt ccccacattt tatccagggc ttactgcata ttcccaacag
     1201 ccattcgtta gccaggtaga cgaataccct gatttgccgc tggttttgct ttcctatcga
     1261 agggcccata catcatcacg cccacgaaac aaccgacgaa ataacgcaac tttctcatcc
     1321 gtggataata cgaaaacgca ctgcacaggg ctctgcggtt tacgtcgcca ttcaatccca
     1381 tgagcttcaa gtaatgagac cagccgggta ttttctgccc gcagtactgc tagttcatcg
     1441 ctgtcagatt tataaaccat atgcgttatc acttgtacct gtctcccggc ggcaccacga
     1501 ctgaaattcg tgtgaaggca aatccacgcg ataacttacg atagttcgga cataagcccc
     1561 cataattcag ttatgtcgtg gtcgaccccg gttacttttt tcgggaagcc tgctacggcc
     1621 cagaataata ccgtgttctt tctgatcctg tttagccaga tagctaaggg cgtaacgtaa
     1681 gtcgtctacc gcagatttat cactatagtg aatcacgtga tcgatacgca ccgggtattt
     1741 gtctttagcc cggcacagat gaaaataacc ctccccttcc gtgatccgcc tccagatatc
     1801 tcccagttgc ctggatatct gataagagtt cttgtgacgt tgtccatcca ggtagccgat
     1861 aaaatgaata tgaagccctt tgtctggtgt atattccatt acccagtaat atcccgccaa
     1921 catcgtctgg gtttcgctaa gtaaacggta tatttccata cacatactgt gcttacagga
     1981 atgcccgaaa ctgggcgtgt ctttcctgta agcaaaatca attctgaaag gtaacagttt
     2041 agaaaaacgt tgaaacatac catccatgtg ttcattcacg tctttcagaa tcatgaaatc
     2101 cattttgtaa ttgggattgg cattatacat tttatagtcc ttactttata aaactcacag
     2161 gaagagagaa ttacgtaaat actgagagta gagaaacgcc gtaccacggc attaataaac
     2221 gaagtaaacc agcagcacag acaacctgat aaaactgttt tgtattaccg cattactgtg
     2281 atgaggtaat acttcagaat acagtgagac tctcttaaac tgagaaccag tacgaattac
     2341 aatggaaaaa cacacctcat agaattgagt ttcaggaagc accactaaat tctttacagg
     2401 aagtaatctg acattcacaa aactcagtat cggctagcta caggcaacat cgctattaat
     2461 attgtcgaac cattaagtaa agccgagaga gaccacgggt atttaatcat tagcataaaa
     2521 gaataatggt atacagaaca tacccacaca ccataagaag attgttccat acagaaaaga
     2581 tcatatttac ctatacctat aaactgtaac ggagtcataa cacgctctaa tagtgttccg
     2641 atacgtaacc agtgttaagt acgtacagga caacattaca cacataattc accatacagc
     2701 ttcccccata tgcttcactg cgtagtgtat ggagatatat gcattacaca catacagaat
     2761 acttagcata aagaggagtg acattaccac gtaaagagat aagttataca acaaacatac
     2821 aaacaatacg gcacagacac acaataccat aactccaact aataatacta attaacctta
     2881 attatttatg gtatatgaaa taaatgtgat tctcccggaa ctaactaacc taaccagaca
     2941 gatgatcact gtataaaaac ttcagttaat actcatttgt tttgtcgact cagtatataa
     3001 tgagtgacca gtaatcagga taatcacact tacataacct gtaaaaaaga acaagaagaa
     3061 taagccacta acataatgca aaatggcatc tgaatcgatc gcagtttggc attcgaatcg
     3121 atcatgattt ggcccaatcc gtgatcgaat agagcggaat acgcaacata attcaccaga
     3181 caagagctta caccgaatta cagattatgt attaatgtca aaactggcat attttacaga
     3241 tacaatgaac atgcaaccca tcaatctaat aggggcggta tgtacatctg cccatgaatg
     3301 ggatggcgct aagaatacaa aacaattaga catagatcac aaaaacacac cattctaatt
     3361 gaaaaacaaa acaaacaaat aaatcatact attatagaat tttctcttgc cagacataga
     3421 taatacccct gctaattttg ctaatggtta gcaaaattag cagggtatat aaagattatt
     3481 gacccaaaac acactcagat gaattcacga cagctcagga gggttattcc tgatgtcatc
     3541 ttcacaaata atggttaacg catcatatat cattctgcgt ctgttcctgt tttcggcctt
     3601 atcgccatac aacatcatca catttttaat ttcatcgacc ggtgatttca catagtgaac
     3661 taatacacca agcttctcaa cacattgcag taaagtatat ttcttactgc ctgtgaaaag
     3721 ccctatcata gtttctttac gttcgcgact ggataatact ttgttttcca taatgacttc
     3781 tgccagcgtc ttaccatcat aaacatactc tttatctatc agaatattta tcttatccag
     3841 caccttctct acgtcatcaa gcaaacctgt aatatatggg ggcgcaatat atggtttcgt
     3901 actgatcttt tcaagcatac aattatacat cttaataaca cgcatcaggg taaaattgtt
     3961 cgcatcatac tgtatttcac cctccttata tacaggatca ataagtggta ttttttcaat
     4021 aaatgcatcc tgataggctt ttacatggtc aagccaataa aacaacgagc catactttat
     4081 attttttcta tttactttaa cctccagtgc tgtccgaagc aacgataatg cccccctgac
     4141 atatcctgaa ggtaattcac caaacaatcc ttttctttcc atgtcatcat aatgctgcag
     4201 cacatcatta attttatttt tccttatcag acaaatgtac accgcaagtg tttcgtagca
     4261 gctggtcaca tgaacagcct cattctgcaa ctgcccaaaa tagcttataa tctttttcag
     4321 atatgcgtca tcacaaccat tcttatcatt aatattaaat acaagatctt tcagacggga
     4381 aatatattcc tgagatttat catcaagtga aacactataa cgagacaatg ttccttctgc
     4441 actgttttga aacttataaa acatttccag taattttttt gcataaggcg ttgtatcaaa
     4501 gctggtaaag tcataaagat attgcgcagc gtaaccatac aaaccatgaa tccagagtcc
     4561 ttctctcacc aacactctga gataatatgc aaggctttga agtatcttcg tctttcgttg
     4621 tatatcaagt aagacactta atgaataaaa tgcattatag aatttatcaa tattcctgag
     4681 cactttctcc agttcatcct tatccagctt cttctcccgg taatcctttt tatattcatt
     4741 tttgatagcc atgatatatt gttgcatcag gctcttgctt ttcccgttat ccttccgtat
     4801 agttcctgta tatttcccca gagcatacag ctttccattt tcatgaaaat cttccttgtt
     4861 gaatcccagt tcagccagat attcccgaag ataacgttta acagcagctt catcgctttc
     4921 tgtatcagcg tgatcaagac aaggacaata cgatttctgt aaaaaattat aaatatgata
     4981 ataaaaagca gagtcattct ctggtctgta tgtattatac atatctacaa caagaaaaat
     5041 aaccccaaaa gtgcgcagac aatagctgcg aaggaattca ggttttagtg tggaatgcac
     5101 tggataagaa ttcatcatgt aataaaaatg ttcatatcgg gcatatatga tccgccgttg
     5161 ctcctgactg aacacccctt cagcatcaat aatacgactg atctcattat aacgttgatc
     5221 ccagaatttt tgatgcattt caaaattggt actcttccgc ttgtgtgccg gattctcgcc
     5281 ttccttaaac aaccccaaaa cagtacaaat atcatacaac atatttgtaa atgtgcgcat
     5341 atgctgtgct ctccggttgg tttgcatttt atcgcagagg acattctgag cttaacgtat
     5401 caaactgaca caacgacatg caatgcatat ggagaacaat atgtcaaatc aaaccggaca
     5461 gctttacacg ccttcacgca tcctgcgtat ggctgaactt acccgactgc tgggtatatc
     5521 aagatctact atttacgtga aaataaatcc ggcctcgaaa taccacgacc ctacctttcc
     5581 caaaccaata aggcttggta gtgcatccgt aggctggcgg gcatctgcca tcgatgagtg
     5641 gctgatgtta catacggcac cagcctggtc tgaacctgaa aacaaactaa aggaacttaa
     5701 tgatgacgac aaaactatca ctccctgaac ttgacacaac taaactgcct gacaggtgcc
     5761 aggcattact tgatgaaatg catgaagaaa caggaatacg ccgtgaaata cttctgtctg
     5821 ttatgctgac ggtcatggct gcctcggtcc aggatacgca tgaagttgaa ctttccggag
     5881 ggcaacgtac cagccttcag atatttatgt gtctttcatc agcatcaggc agtggaaaaa
     5941 cctctgcctg cgcaaaatta atcgctcctg ttcatgaaac agaagaagaa ctacatcagg
     6001 catatatcga cgataaaaaa aactatgacc gaatgatgga aatgtggaca acagataaaa
     6061 aaatcctgga gcggagatat aaaaaggaaa ttgaaagatc cccggaaaat gctgctgctg
     6121 cacgagcagc acttgaaaag tgtatagcaa ataaacccga acctcccgta caacaggttc
     6181 tcatcgtgaa tgacgcgaca ccagaaggga tagctttgaa actcagccag tcaccctctc
     6241 ttctgttgct gtctgatgaa ggaggtacta ttctggataa gcgtttcgaa cgcaaatcgg
     6301 cactgtacaa cacattatgg agtgggcaac cagtcaacgt tgaacgggca tccagacccg
     6361 ggttccggct gaatgatgtc cgtctcacta tacttatact gactcaaccg gtaatattta
     6421 ataagttttt tactctcact ggcgatcaga tccggggcaa tggctttctg gcccggttgc
     6481 tattttgcga acccggagca attaaaataa tgacgacaga accgcatact gccactcctg
     6541 ttgtgacgca acaatgtgca agctgttttc gtaagaaaag ttttggcagt cagatcagag
     6601 actctctgag agcatccaga gaaagacgtg caaaaagtaa agagcgcatc tgcatgacat
     6661 tatcaaatgc agcatcccgc gcactggagc gctttcacaa agaaaatatg gatactgtca
     6721 gacagaatcc tcatatgggt acatttgaag acattattgt cagaaaaaga gagcaggttg
     6781 tcaggatagc agcacttctg gaactggaga aagatccaga ctcgacagta ataacgctgg
     6841 aaagtactaa tagcgccatt tatctcattg aattttattt caagcatctt atatataaac
     6901 ttgaatccct tcgggaaata tccccggctg agaaacttga taaatggctt cagaaaagaa
     6961 ttatcacaac agcaggttac atattccaga aaagttatat tttacaatat gccccatatg
     7021 ctctcaggaa aaaatgtgtt ctggatgaag cacttgacat actggcggaa caaaggaaaa
     7081 taaggattga tgataacctg gttgtttata taggaaacac gattacacca tccgaactgg
     7141 caaaaaaact aaatattcct gcattcgatg ccggagtttt catttgtgat catcaaaaca
     7201 tactgaagta tcaccgaaac agactataga tactatcgga caaaaaataa attatcacaa
     7261 gtgtattatt cgttataata atgaataatc gttatctgag tgcaatgcca ggttttcatt
     7321 acgaactggc attgcattta tttttcaaaa aagaaagcag taaccatttg gctactctga
     7381 gtatgccagt ccatgaaact aagcgaattc cttagtgttt ttagcaagtt gagacatcac
     7441 ataatccgcc catgcctgca tcatcggtac acgctgctcc agtagatcag tacgatgata
     7501 tgccgcctca accttatttt tcagcgtatg agcgagcgcc ctttccgcca gatcccgcga
     7561 atacccctgt tcactacacc agtccctgaa tgttgagcga aaaccatgtg ccgtggcaac
     7621 tcgccccgga atgtcactga cggctttctt tttacgcaga aaacttgtca acaccatatc
     7681 ggaaaggatc tgctgctttc tgggtgaagg gaacaccagt tcatcatgca ggccacgtat
     7741 attttccaga atgtaaatag cctgccggga taaaggaaca cgatgctgta gcctagcttt
     7801 cattctttct gcaggtatag tccatacccg cttatgaaaa tcaatttcag cccagcgcat
     7861 tcccctggct tcgcccgagc gagttgctgt aagtatcacc attaataaca gtgcgcgggt
     7921 aacattataa ggttcatcac tatacacact ggtcgccaca aaaagcggta actgcctcca
     7981 gggcattgcg ggttggtgtt catcacgtcc tcttgtctgc tgaggaagca aatggtcaac
     8041 cacatcaaca ggatttgcca cacaaaaacc gtgcgcccat ccccactgca taacaacatg
     8101 aatgcgctgt ttaacccggc ttgccgtttc tgacaaggtt aaccagactg gacgcagtgt
     8161 ttctgccacg tccgcagccg taatcgaatc cagcgttttt gctcccagtt gaggaaacgc
     8221 gtaattctca agcgtcgata accactgcct tacatgcttt ggattctccc atccaggaga
     8281 cagttctgca tgtacacgcc tggctgcatc ggcaaatgtt gggataacga ctttctcaga
     8341 ttcagccttt ttaatctcca gaggatcatc acctgcagca agttgctctc gcattatccg
     8401 ggcagtacgt gcagcttcag caatactgac ctctgggtaa gttcccaatc cagcattacg
     8461 tcttttttgt gtcaccggac ttacataacg aaaaacccat ttcccccgcc cctttactga
     8521 agaaggatga agggtcagtc cggtaattcc cccatggggc aatggtttgt catcaggttt
     8581 gatatgtctt gctttcgtat ccgtcaatac tgccatacgc taattcccgt cactctggta
     8641 tgccatccag tatgccatta gtccctcgct tcactaagaa tacattagat aacatcggac
     8701 aacaaaaagt gtaatacatt gtttataaag aaattatata acacccctgg attaagtcag
     8761 atttatttca ggcggtcccc ctcaccgcca tatttaaaga agagcgcgta cgaaagtacg
     8821 ggcttttttt tcgtatattg tacacaccgg gtgcccccgc ccacgtctgt tagggcgagg
     8881 ggaaattgtg cacatcaact gtctggttac tcagtcaaca atcaaaatgc gcaagatagt
     8941 acagaacata tatgcgttat ctggtcactt cctgtaactt cctcacttat taaaatgccc
     9001 ccaacggaaa tacatgtcac atgccatcat aaaatcgtta tgccagagga atcgacaatg
     9061 gaaaaaatag taaattaagc acggtacata gcagagtaaa aaataggctt caaaccaaat
     9121 ccgccattcc acaagcgttg ccaataccga caccagcgta atccacatca tttcaataac
     9181 cagcaatatg aataaccgaa gccaaagcgg cttggcaaaa aaggtcgaca acgtattttc
     9241 actggagaaa aacagatcga tttcctgcaa aaaaaacctg cccagaaaag cgtaccgctt
     9301 ggagaagcgc agaaaaaaca gattaatcgc caacactacg ccacaaagaa tgagcaatgc
     9361 aattatcgat ttaaacgtta accactgccc ctctgtcaca aaagaatcac tgttacgcaa
     9421 cgggctgata gtaagcaggt taacaaccgt tgagatgcac accgaaagca gcacatagcg
     9481 agtcagactt ttatatttta cttttttcgc tactgaaata gcgacctgct tgacataact
     9541 ggtgcggatc atccgaaaca gcagaaagat gaccaacggc ccaagtaacg aaatcaggaa
     9601 cagcgcatat aacgagggta gaagctggta tagcccccaa ctcattgcac tcaacatgat
     9661 taacgacaga aatcctgcca ccagaggttg tgtaagaaat aattctattt tgtggttctg
     9721 ctttttctta aaccacaaaa aatcgatatt cttcgtgacg catttataag cccagtacgc
     9781 cttgagatca aaaataaaat tatacagcac cagggccaga atcccactaa ctacaccaat
     9841 atcctctgat ggaaaagtaa accctgcatt tttccaataa aagcctatca gaataagtcc
     9901 aatagcataa atgtatatca gatttcgttt atcctgaaag cgaataaaag ttaaataatc
     9961 aggaatcatt tgaatacctt tgcatcacta aatgctttac gacaatgcat aactcgttgc
    10021 tgatccacgc cagcaaaatc atttaacata ttccccaaat agggcttatt acttatttc
//
LOCUS       DAJTYF010000130        10034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTYF010000130 DAJTYF010000000
VERSION     DAJTYF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.130"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..661
                     /locus_tag="OO590_003461"
     CDS             <1..661
                     /locus_tag="OO590_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-2-oxo-heptane-1,7-dioate aldolase"
                     /protein_id="HCR6638701.1"
                     /translation="NVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLV
                     PMVQNADEARKAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIET
                     REAMKNLPQILDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKA
                     PGILIANEQLAKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSV
                     Y"
     gene            683..2038
                     /gene="hpaX"
                     /locus_tag="OO590_003462"
     CDS             683..2038
                     /gene="hpaX"
                     /locus_tag="OO590_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6638702.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2048..2938
                     /gene="hpaA"
                     /locus_tag="OO590_003463"
     CDS             2048..2938
                     /gene="hpaA"
                     /locus_tag="OO590_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6638703.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            2965..3902
                     /locus_tag="OO590_003464"
                     /pseudo
     CDS             2965..3902
                     /locus_tag="OO590_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4129..5687
                     /gene="hpaB"
                     /locus_tag="OO590_003465"
                     /pseudo
     CDS             4129..5687
                     /gene="hpaB"
                     /locus_tag="OO590_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5705..6217
                     /locus_tag="OO590_003466"
     CDS             5705..6217
                     /locus_tag="OO590_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6638704.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6610..8760
                     /gene="btsT"
                     /locus_tag="OO590_003467"
     CDS             6610..8760
                     /gene="btsT"
                     /locus_tag="OO590_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6638705.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            8810..9013
                     /locus_tag="OO590_003468"
     CDS             8810..9013
                     /locus_tag="OO590_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6638706.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9024..9980
                     /gene="yjiA"
                     /locus_tag="OO590_003469"
     CDS             9024..9980
                     /gene="yjiA"
                     /locus_tag="OO590_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6638707.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2387 a         2619 c         2583 g         2445 t
ORIGIN      
        1 taacgtgcaa accgtgctca cccaactaca ggcgattgcg ccctacccca gccagccagt
       61 ggtacgccca tcgtggaacg atccggtgca aatcaaacaa ctgctggacg taggcacaca
      121 aaccttactg gtgccgatgg tacaaaacgc cgacgaagcc cgtaaagcgg tacgcgccac
      181 ccgttatccc cccgccggta ttcgcggtgt gggcagtgcg ctggctcgcg cctcgcgctg
      241 gaatcgcatt cctgattacc tgcaaaaagc caacgatcaa atgtgcgtgc tggtgcagat
      301 cgaaacgcgt gaggcaatga agaacttacc gcagattctg gacgtggaag gggtcgacgg
      361 cgtgtttatc ggcccggcgg atctgagcgc cgatatgggt tatgccggta atccgcagca
      421 cccggaagta caggccgcca ttgagcaggc gatcgtgcag atccgcgaag cgggcaaagc
      481 gccggggatc ctgatcgcca atgagcaact ggcaaaacgc tatctggaac tgggcgcgct
      541 gtttgtcgcc gtcggcgttg acaccaccct gctcgcccgc gccgccgaag cgctggcagc
      601 acggtttggt aatagttctt ctatttcgat ggcgaagcaa aacaacaact ctgtctacta
      661 aacgtacgat gtggagcgca tcatgacgac ctctaccctg caatataaag ataataaaac
      721 tgttgaaatt gaacatcgtg tcattaataa attatttcgt cgcctaatca tttttctttt
      781 tattctgttt gttttttcgt ttcttgatcg catcaatata ggatttgccg gactgacaat
      841 gggcaaggat cttggcctga cgtccacaat gtttggcctc gccgcgacac tcttttatgt
      901 cacgtacgtc atgtgcggga tccccagtaa tatgatgtta gcgaaaattg gcgctcgtcg
      961 atggatcgct gggatcatgg tggtctgggg aattgcttcc acctgcacaa tgtttgcaac
     1021 aagccccgaa acactctatc tactgcgtat gctggttggt attgctgagg cgggtttttt
     1081 acctggtatt ttggtttatc tgacctggtg gttcccggca tatcatcggg ccagagccaa
     1141 tgccttattt atgatagcga tgccagtcac tatgatgctg ggattgatcc tttcaggcta
     1201 cattctggcc ttggatggac tgtggaatct aaaaggctgg caatggctct ttttgctgga
     1261 aggtctgcca tcggtcattc ttggtgtcgt cacctggttt tatctcaacg acacaccaga
     1321 ccaggcgacc tggcttgatg aaaacgaaaa acaggcttta aaaacgatga ttctccgtga
     1381 acaagagagt gcatttgccc atgcatctgt tccacgctca accttacgtg aagtcctgac
     1441 cccagccatc atgctatata cacttgctta tttttgcctg actaatactc tgagtgcgat
     1501 taatatctgg acgccgcaaa tcctacagag tttcaatact ggcagtagca acatcatgat
     1561 tggtttgctg gcggccatcc cccaattctg cacaattttt ggcatgatct ggtggagtcg
     1621 tcgttccgac cgtcttaaag aacgcaaaaa gcatacgatt cttccctacc tgtttgccgc
     1681 tgccggatgg ttactcgctt ctgcgacaga tcacagcctc attcaactga gcggcatcat
     1741 tatggcctca atgggatcat ttaccgcgat gactatcttt tggacaacac aggatcaagt
     1801 tataagcttg caatcgcgtg ccgttgcgat ggctgtcatc aatgctgtcg gcaatgtagg
     1861 ttcagcagtc agtccattac ttatcggtat tttgaaagat gcaaccggca gttttagctc
     1921 aggtttgtgg tttgtagcag gattgctggt actgggcgcg ttagtcatga cccgaatccc
     1981 tatggggagt cgggaacact ctctttcatc tgaacttatc gcaccaaaac cacaataagg
     2041 agtcatgatg tccagtgaag ctatctttga acatggttat gaaaatattg atttggtccg
     2101 ggaatatgat gcccgctacg ctggtgaaga tgtgcattat gaaacattta cccggttagc
     2161 gaatttcttt ggacgcgata tgcgccctca ctggcacgat agatattttc aactccatta
     2221 tctggtcact ggacgcatta ctttacaact ggatgagcac ttctattctg tgcgcgcacc
     2281 gctttttgta ctaacaccac catcggtgcc acatactttc tttactgatc ctgatagcga
     2341 tgggcatgtt ctgaccgtac gtcaggagtt aatttggccc ctggtagaaa agctctggcc
     2401 tggtagtgga gatgccgcaa tgctacaagg tatctgtctg tcaccggagg gaatggaaca
     2461 tacactggcg gcattaaatc attattggcc gataatatcc ctggagtttc aacaaaataa
     2521 gccaggacgg gaaattctat tgcaatcact ggcgcaaagt atttttacaa tcctactgcg
     2581 tgaagcacca ccaaatgata tttcaacatg cagcgtgcgc ggcgagatgt gtttattcca
     2641 gaagtttaat cggctgattg acgaaaatta tcgccaacat ttaatggtcc ctgaatatgc
     2701 aacaatgctc ggtatgagtg aatcacggct taccgagctg tgccgacgtt ttgccaatca
     2761 atcgccgaag agattgattt tcgaacgagt gagccgggaa gccagacggc ttctgctcta
     2821 tagcgagcaa agcatcaacc agatagccct tgatcttggc tacaaagatc ccgcctattt
     2881 cgcccgtttt ttcaaccgaa tgatgggttg ctcaccaaca caattccgtg gtcgctgacc
     2941 atttatcaac ctgagagact cgacatgaaa atatctttac gctcactatt tgtggcgtgc
     3001 ctgattgcgc cagtggcatt cacctacgcc tccgaaccgc aataaatgaa aattgtacta
     3061 cttggaacga aaggtggccc ttctttatta aaagctgacc gtttaccgat ggcaacggca
     3121 cttattattg gcaataaaac ctggttaatt gatgctggtt acggggccag cttacaactg
     3181 gtaaaaaata atattccact gcgccagatt gataatattt tactcaccca tttacacagt
     3241 gatcatattc tggactatcc atcagtctta atgaatgcat gggctagcgg tctgcaggac
     3301 aaaactattc atgtattcgg tccgccaggc acccgagcca tgaccgctgc cagttggcag
     3361 gtcttcgatc gggacatcac actgcgtatg gaagaagaag gaaaacccga tcctcgcaaa
     3421 ctcgttacag ctcaagatgt tggtgaaggc gtagtttata aagatgatac cgtgaccgtg
     3481 actgcattaa aggtccctca ttctcctttc cctgacggtg aggcttttgc ttatcgcttc
     3541 gacacacaag gcaagcgaat tgtcttctca ggagatacct cgtactttcc cccactggca
     3601 actttctcag aaggcgctga tatacttgtg catgaggccg tacatgagcc ctccgtcgct
     3661 aagctggcag acagtattgg caatggtaaa acactggcaa aagccattgc ttctcaccat
     3721 acgactattg aggatgtagg caaaattgcc aatgaagcaa aagtgaaaaa actcatcctt
     3781 agccacctgg taccaatcac cgttccggat gaaacatgga agcaagaagc acagaaaact
     3841 attcagtccc tatcgtcgtt ggtcatgaaa atatgacgat taccattcca ttcgccccat
     3901 aaaaaattcc gtccccgtta gcccttgtta gcggggaata ttatgatttg ccttactgta
     3961 agttgatcac gatcgcagtt tactatttaa ctccaccttg ctgtcgaaaa gtaccaacgt
     4021 tcgcctgaac cgtctcttcc cgacaaccgc gttcaacgct tcaattaaat aacaaaaaca
     4081 acacacaaaa ttaacaatcg tattccgatt aatactgtag aggtcgacat gaaaccagaa
     4141 gatttccgcg ccagtaccca acgtcctttc accggggaag agtatctgaa aagcctgcag
     4201 gatggtcgcg agatctatat ctatggcgag cgagtgaaag acgtcaccac tcatccggca
     4261 tttcgtaatg cggcagcgtc tgttgcccaa ctgtacgacg cgctgcacaa accggagatg
     4321 caggactctc tgtgttggaa caccgacacc ggcagcggcg gctataccca taaattcttc
     4381 cgcgtggcga aaagtgccga cgacctgcgc cagcaacgcg acgccatcgc tgaatggtca
     4441 cgcctgagct atggctggat gggccgtacc ccagactaca aagccgcttt cggttgcgca
     4501 ctgggcgcga atccgggctt ttacggtcag ttcgagcaga acgcccgtaa ctggtatacc
     4561 cgtattcagg aaactggcct ctactttaac cacgcgattg ttaacccacc gatcgtcatt
     4621 tgccgaccga taaagtgaaa gacgtttaca tcaagctgga aaaagagact gacgccggga
     4681 ttatcgtcag cggtgcgaaa gtggttgcca ccaactcggc gctgactcac tacaacatga
     4741 ttggcttcgg ctcggcacaa gtgatgggcg aaaacccgga cttcgcactg atgttcgttg
     4801 cgccaatgga tgccgatggc gtgaaattaa tctcccgcgc ctcttatgag atggtcgcgg
     4861 gtgctaccgg ctcgccatac gactacccgc tctccagccg cttcgatgag aacgatgcga
     4921 ttctggtgat ggataacgta ctgatcccat gggaaaacgt gctgatctac cgcgattttg
     4981 atcgctgccg tcgctggacg atggaaggcg gtttcgcccg tatgtatccg ctgcaagcct
     5041 gtgtgcgcct ggcagtgaaa ctcgacttca ttacggcact gctgaaaaaa tcactcgaat
     5101 gtaccggcac cctggagttc cgtggtgtgc aggccgatct cggtgaagtg gtggcgtggc
     5161 gcaacacctt ctgggcattg agtgactcga tgtgttcaga agcgacgccg tgggtcaacg
     5221 gggcttattt accggatcat gccgcactgc aaacctatcg cgtactggca ccaatggcct
     5281 acgcgaagat caaaaacatt atagaacgca acgttaccag tggcctgatc tacctccctt
     5341 ccagtgcccg tgacctgaac aatccgcaga tcgaccagta tctggcgaag tatgtgcgcg
     5401 gttcgaacgg tatggatcac gtccagcgca tcaagatcct caaactgatg tgggacgcca
     5461 ttggcagcga atttggtggt cgtcacgagc tgtatgaaat caactactcc ggtagccagg
     5521 atgagattcg cctgcaatgt ctgcgccagg cacaaagctc cggcaatatg gacaagatga
     5581 tggcgatggt tgatcgctgc ctgtcggaat acgaccagaa cggctggact gtgccgcacc
     5641 tgcacaacaa cgacgatatc aacatgctgg ataagctgct gaaataacgc agcaggaggt
     5701 taagatgcaa ttagatgaac aacgcctgcg ctttcatgac gcgatggtca gcctgtcggc
     5761 agcggtaaat attatcacca ccgagggcga cgccggacaa tgcgggatta cggcaacggc
     5821 tgtctgctcg gtcacggata caccaccgtc gctgatggtg tgcattaacg ccaacagtgc
     5881 gatgaacccg gtttttcagg gcaacggcaa gttgtgcgtc aacgtcctca accatgagca
     5941 ggaactgatg gcgcgccact tcgcgggcat gacaggcatg gcgatggaag agcgttttag
     6001 cctctcatgc tggcaaaaag gtccgctggc gcagccggtg ctaaaaggtt cgctggccag
     6061 tcttgaagga gagatccgcg atgtgcaggc gattggcaca catctggtgt atctggtgga
     6121 gattaaaaac atcatcctca gtgcagaagg tcacggactt atttacttta aacgccgttt
     6181 ccatccggtg atgctggaaa tggaagctgc gatttaagct ccagcccctt cacgcattcg
     6241 tcgaaggggc atttttattg tgatctcaat cacatatcta cccgatgaat aattcttgcc
     6301 caaccgctca ccgccattcc tgaccgctta catccctaaa ataaccactc agttatttac
     6361 cttactttac gcgcacgtat ctctggcaac atcactgcag gatagcggtc aatttacctc
     6421 ctcaaacgca acgcaaacct agaacggctt cggcctatga ttaacaaatt actctatgga
     6481 ttttggtttg caggaaggca gcaagtgagt gaatccccgg gagcttacaa aagtaagtgt
     6541 cagaggtgaa cgaacgcagc tgccgcacct gtaagccaaa agacgacgag taaaatgcca
     6601 ggttttacta tggatacgaa aaaactattc aagcacatac cctgggtgat tctcggaatc
     6661 atcggtgcat tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt cagcgccctg
     6721 tggatcgtgg tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta cagtctgtac
     6781 atcgcccaga aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt tattaacaac
     6841 gacggtctga actacgttcc gaccaaccgt tacgtgttgt ttggtcacca cttcgccgct
     6901 atcgccggtg ctggtccgct ggtgggaccg gttctcgccg cgcagatggg ctacctgcct
     6961 ggcacgctgt ggctgctggc gggggtagtg ctggccggtg cggtacagga ctttatggtg
     7021 ctgtttatct cctctcgccg taacggcgca tctctcggtg agatgatcaa agaagagatg
     7081 gggccagtac cggggactat cgcgctgttt ggctgtttct taatcatgat catcatcctc
     7141 gccgttctgg cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg tgtcttcacc
     7201 gtttgctcaa ccgtaccgat tgcgctgttt atgggtatct acatgcgctt tatccgtccg
     7261 gggcgtgtgg gtgaagtctc tgttattggt atcgtgctgc tggttgcctc tatctacttc
     7321 ggtggcgtga ttgctcacga tccgtactgg ggtccggcac tgacctttaa agacaccacc
     7381 attaccttcg cgctgattgg ctatgcgttt gtttccgcac tgctgccggt gtggctgatc
     7441 ctcgcaccgc gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt cggcctggcg
     7501 ctgggtatcg tggtgctgaa cccggaactg aaaatgcctg cgatgaccca gtacattgac
     7561 ggtactggcc cgctgtggaa aggcgctcta ttcccgttcc tgttcatcac catcgcctgt
     7621 ggtgcggtat ctggcttcca cgcgctgatc tcttccggta cgacgccgaa actgctggct
     7681 aacgaaaccg acgcgcgttt cattggctac ggcgcaatgc tgatggagtc cttcgtggcg
     7741 attatggcgc tggttgcagc gtccatcatc gaaccgggtc tttacttcgc gatgaacacc
     7801 ccgcctgctg gccttggcat caccatgcct aacctgcatg aaatgggtgg cgagaacgcg
     7861 ccgatcatca tggcgcagct gaaagacgtt accgcacacg cggcagcgac cgtcagctcc
     7921 tggggcttcg tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat tggtgagcct
     7981 tctgtcctga accgtgcagg tggtgcgccg acgctggcgg taggtatcgc tcacgtgttc
     8041 cacaaagtgc tgccgatggc tgacatgggc ttctggtatc acttcggtat tctgttcgaa
     8101 gccctgttca tcctgaccgc gctggatgcg ggtacccgtt ctggccgctt tatgctgcaa
     8161 gacctgctgg gtaacttcat cccgttcctg aaaaaaaccg actctctggt tgccggtatc
     8221 atcggtactg cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg cgtggtcgat
     8281 ccgctgggcg gcgttaagag cctgtggccg ctgttcggta tctccaacca gatgctggca
     8341 gccgtagcgc tggtactggg caccgttgtg ctgattaaga tgaagcgcac ccaatacatc
     8401 tgggtaactg ttgttccggc tgtatggctg cttatctgca ccacctgggc gctgggtctg
     8461 aaactgttca gcaccaaccc gcagatggaa ggcttcttct acatggcaag ccagtacaaa
     8521 gagaagattg ctaacggtac tgacctgacg gcgcagcaga ttgccaacat gaaccacatc
     8581 gttgtgaaca actacaccaa cgcaggcctg agtattctgt tcctgattgt ggtgtacagc
     8641 atcatcttct acggtttcaa aacctggctt gcggtgcgta acagcgacaa acgtactgac
     8701 aaagaaacac cgtacgttcc aatcccggaa ggcggcgtga agatctcttc gcaccactaa
     8761 ccgtgtttag ccccgcttcg gcggggcttt gttcttatca gagtgaacta tgtttggtaa
     8821 cttaggacag gcaaaaaaat atctcggcca ggcggcgaag atgttgattg gtattccaga
     8881 ctacgacaac tacgtcgagc atatgaagac caaccatccc gacaagccgt acatgagcta
     8941 tgaagaattc ttccgcgaac gccagaacgc gcgctacggc ggcgatggta aaggcggtat
     9001 gcgctgttgt taatggagag agcatgaacc cgattgcagt taccctactc accggttttt
     9061 taggcgcggg caaaactacc ctgctgcgcc atattcttaa cgaacaacac ggctacaaga
     9121 ttgccgtgat tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg attggcgacc
     9181 gcgccacgca gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc tccaacgagc
     9241 tggaggacgc actactcgac ctgctggata atctcgacaa gggcaatatt cagttcgatc
     9301 gtctggtgat tgaatgcacc ggcatggccg atcccggccc gattattcag acctttttct
     9361 cccatgagat tttatgccag cgctacctgc tggacggcgt gattgcgctg gtggatgcgg
     9421 tacatgccga tgagcagatg aaccagttca ccatcgccca gtcccaggtg ggctacgccg
     9481 accgtattct gctgactaaa accgacgtcg caggcgaagc agagaaactg cgtgaacgcc
     9541 tggcgcgcat caacgcccgc gcaccggtct acaccgtcac ccacggcgac atcgacctgg
     9601 gcctgctgtt caacaccaac ggttttatgc tggaagagaa cgtcgtcagc accaaaccgc
     9661 gtttccactt tatcgcggat aaacaaaacg atatttcgtc aattgtggtg gaactggatt
     9721 acccggtaga tatcagcgaa gtttcccgcg tgatggaaaa cctgctgctg gaatcggcgg
     9781 ataaactgct gcgttacaaa gggatgctgt ggattgacgg cgaacctaac cgcctgctgt
     9841 tccagggcgt ccagcgcctc tacagcgccg actgggacag gccgtggggc gatgaaaaac
     9901 cgcatagcac aatggtgttt atcgggattc agttgccgga ggaagagatt cgggcggcgt
     9961 ttgcgggatt gaggaagtaa gttcgggaaa gatcaacttt ttgagagttc caccgcaaca
    10021 ttattacgag tgga
//
LOCUS       DAJTYF010000131         9947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTYF010000131 DAJTYF010000000
VERSION     DAJTYF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.131"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO590_003470"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO590_003470"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6638708.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO590_003471"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO590_003471"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6638709.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="OO590_003472"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="OO590_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /locus_tag="OO590_003473"
     CDS             3456..5216
                     /locus_tag="OO590_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6638710.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5622..6479
                     /gene="focA"
                     /locus_tag="OO590_003474"
     CDS             5622..6479
                     /gene="focA"
                     /locus_tag="OO590_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6638711.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6534..8816
                     /gene="pflB"
                     /locus_tag="OO590_003475"
     CDS             6534..8816
                     /gene="pflB"
                     /locus_tag="OO590_003475"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6638712.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9008..9748
                     /gene="pflA"
                     /locus_tag="OO590_003476"
     CDS             9008..9748
                     /gene="pflA"
                     /locus_tag="OO590_003476"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6638713.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2551 a         2493 c         2373 g         2530 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acatcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagagc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccagg
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcaacgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgccccg ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata
     5341 tcggtaattt gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc
     5401 ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta
     5461 aaatttattt ttatttggat aatcaaatag ttactccgta tttgcataaa aaccatgcga
     5521 gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc tataatgctt
     5581 tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt
     5641 ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag
     5701 caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa
     5761 tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg
     5821 caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag
     5881 ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct
     5941 ggggtcagtt ggctaaaaac tggctaaatg tctattttgg caatctggtc ggcgcactgc
     6001 tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac
     6061 taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc
     6121 ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca
     6181 gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt
     6241 ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg
     6301 catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg
     6361 tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg
     6421 gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat
     6481 ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg
     6541 agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg
     6601 aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct
     6661 tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta
     6721 aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca
     6781 cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg
     6841 aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct
     6901 gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta
     6961 aaactcacaa ccagggcgta ttcgacgttt acactccgga catcctgcgt tgccgtaaat
     7021 ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc
     7081 gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct
     7141 ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag
     7201 aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct
     7261 acgacatctc tggtccggca accaacgctc aggaagctat ccagtggact tacttcggct
     7321 acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt acctccacct
     7381 tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc
     7441 aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg
     7501 aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg
     7561 gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca
     7621 ctatgggtcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga
     7681 acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg
     7741 atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc
     7801 cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca
     7861 tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt
     7921 ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact
     7981 tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg
     8041 acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca
     8101 tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg
     8161 cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg
     8221 aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag
     8281 aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt
     8341 ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg
     8401 gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga
     8461 aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg
     8521 gtatctccta caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta
     8581 agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg
     8641 gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc
     8701 cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc
     8761 tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag
     8821 atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt
     8881 ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga
     8941 ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa
     9001 caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg
     9061 cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca
     9121 taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa
     9181 ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg
     9241 cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga
     9301 aggcattcat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga
     9361 tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat
     9421 ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc
     9481 gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga
     9541 cgattcggcg catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga
     9601 acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa
     9661 actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga
     9721 gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg
     9781 ccgctttttt attccgcgct tcgttctgct acagcgtttt taacacatct tatagtgcat
     9841 ctaccatatc ccgtacctga gataataacg actgataata gctcgcgtga agttcttccc
     9901 catcgaatac cactgtatta cacaagaaag tgacatcggc atcttca
//
LOCUS       DAJTYF010000132         9762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTYF010000132 DAJTYF010000000
VERSION     DAJTYF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.132"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..579
                     /locus_tag="OO590_003477"
     CDS             <1..579
                     /locus_tag="OO590_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6638714.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGAAKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO590_003478"
                     /pseudo
     CDS             597..1235
                     /locus_tag="OO590_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO590_003479"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO590_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6638715.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO590_003480"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO590_003480"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6638716.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO590_003481"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO590_003481"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6638717.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO590_003482"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO590_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6638718.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO590_003483"
     CDS             complement(5552..6613)
                     /locus_tag="OO590_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6638719.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO590_003484"
     CDS             complement(6610..8007)
                     /locus_tag="OO590_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6638720.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO590_003485"
     CDS             8163..9161
                     /locus_tag="OO590_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6638721.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..>9762)
                     /gene="ompG"
                     /locus_tag="OO590_003486"
     CDS             complement(9272..>9762)
                     /gene="ompG"
                     /locus_tag="OO590_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR6638722.1"
                     /translation="DVKLTDDLRFNGWLSMYKFANDLNTTGYADTRVETETGLQYTFN
                     ETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYLPLTLGNHSVTPYTRIGLDRWSNW
                     DWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYAFEWQDHDEGDSDKFHYAGVGVNY
                     SF"
BASE COUNT         2473 a         2596 c         2339 g         2354 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgaacaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcggcaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctagctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcggcttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccta ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgctgcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagcgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcaaactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcataggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg
     5701 tcagaagtcc agctccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaggcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgtctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcgtc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagccggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag aaaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actccagcga tccagcccaa
     9481 tgcgcgtata cggcgtaacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc cc
//
LOCUS       DAJTYF010000133         9724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTYF010000133 DAJTYF010000000
VERSION     DAJTYF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.133"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            429..1547
                     /locus_tag="OO590_003487"
                     /pseudo
     CDS             429..1547
                     /locus_tag="OO590_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
     gene            1613..1861
                     /locus_tag="OO590_003488"
     CDS             1613..1861
                     /locus_tag="OO590_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR6638723.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLSAIEYFVLRFIWRRWFSLAD"
     gene            1926..2294
                     /locus_tag="OO590_003489"
     CDS             1926..2294
                     /locus_tag="OO590_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6638724.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPRYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2388..3041
                     /gene="nfsB"
                     /locus_tag="OO590_003490"
     CDS             2388..3041
                     /gene="nfsB"
                     /locus_tag="OO590_003490"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6638725.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3149..4396
                     /gene="ybdG"
                     /locus_tag="OO590_003491"
     CDS             3149..4396
                     /gene="ybdG"
                     /locus_tag="OO590_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6638726.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO590_003492"
     CDS             complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO590_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6638727.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5941..9076)
                     /gene="cusA"
                     /locus_tag="OO590_003493"
                     /pseudo
     CDS             complement(5941..9076)
                     /gene="cusA"
                     /locus_tag="OO590_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9088..9629)
                     /locus_tag="OO590_003494"
                     /pseudo
     CDS             complement(9088..9629)
                     /locus_tag="OO590_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT         2390 a         2695 c         2349 g         2290 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagggttg atagaaatat
       61 ctcctggggc ttcatctttc cagacctcag agtaaacctg aaatcggaaa gcttcaggca
      121 cccgcagtta tcttaccctt cctcaacaaa atagctgttt atttatacag caaatcctgt
      181 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
      241 tcaatcgtca aaattcttcg ccaccagcca gctctgccat aacttgccac attgaaaaag
      301 tagccaaagt gcttcaggca ccatcagttc accatcacca aaaaaaagaa acattccaga
      361 acttccgcat ctcgcccagc cgctccgact tctatactga atagaaaacg tcaacttaag
      421 agaaacctat gccattaccc gattttcatt tttctgaacc ttttaccctc ggtattgaac
      481 tggaaatgca ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga
      541 ttgacgcggt taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta
      601 tgctggagct ggcgacggat gtttgccgtg atatcaacca ggctgccggg cagttttcag
      661 cgatgcagaa agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg
      721 gcacgccccc gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac
      781 gaacgctgga aaactttggc tatctcatcc agcaggcgac cgtttttggt cagcatgtcc
      841 atgttggctg tgccagtggc gatgacgcca tttatttgct gcacggcttg tcacggtttg
      901 tgccgcattt tattgccctt tccgccgcgt cgccatatat gcagggaacg gatacgcgtt
      961 ttgccccctc acgaccgaat attttttccg cctttcctga taatggcccg atgccgtggg
     1021 tcagtaacta gcaacaattt gaagccctgt ttcgctgtct gagttacacc acgatgatcg
     1081 acagcattaa agatctgcac tgggatattc gccccagtcc tcattttggc acggtggagg
     1141 ttcgggtgat ggataccccg ttaaccctta gccacgcggt gaatatggcg ggattaattc
     1201 aggccaccgc ccactggtta ctgacggaac gcccgttcaa acatcaggag aaagattacc
     1261 tgctgtataa attcaaccgt ttccaggcct gtcgctatgg tctggaaggc gtcattaccg
     1321 atccgcacac tggcgatcgt cgaccgctaa cggaagatac cttgcgattg ctggaaaaaa
     1381 tcgccccttc agcacataaa attggcgcat cgagcgcaat tgaagcactg catcgccagg
     1441 tcgtcagcgg tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc
     1501 tgattgggct ggtgaaaaag cattgtgaga tctgggccgg tgactaagcc gggaattgcc
     1561 ttacagaaga atatccctga caatggtgtt ttaacttata tttaacaatg ttatgaaaca
     1621 ccctttagaa accttgacca ccgcagcagg cattttgctg atggctttcc tctcttgcct
     1681 gctgctgccc gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca
     1741 tctgatggat cttagccagc tttacacttt attgttttgt ctgtggtttt tagtgctgag
     1801 cgctattgag tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta
     1861 aacgtcgatt atcgccgaac ttcgcggcat actttgctta ttctccttcg cctaaaggaa
     1921 cgtttatgga taagcaatca ctgcacgaaa cggcgaaacg cctagctctt gagttaccct
     1981 ttgtcgagct ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga
     2041 tttttatgct gtcgtcggag ctacgcggcg tcccctttat caatctgaag tccgatccac
     2101 aaaaatccct gttaaatcag caaatatacc caagcattaa gccaaggtat cacatgaata
     2161 aaaagcactg gatttcggtg tattccggcg aggaaatctc cgaagcgtta cttcgcgatc
     2221 tgatcaacga ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag
     2281 tgcgtccagg ctaaagcggg aatctatagc gcatttttct cgcttaccat ttctcgttga
     2341 accttgtaat ctgctggcac gcaaaattac ttacacatgg agtctttatg gatatcattt
     2401 ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg
     2461 aacaggccga gcagatcaaa acgctactgc aatacagccc atccagcacc aactcccagc
     2521 cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg
     2581 ccggtaatta tgtgttcaac aaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct
     2641 gtgcaaaaac cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgccg
     2701 atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg
     2761 ccgatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc
     2821 tcaacgtcgg taacttcctg ctcggcgtgg cggcgctggg tctggacgcg gtgcccatcg
     2881 aaggttttga cgccgcaatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca
     2941 gtctggtggt tgttccggta ggtcatcaca gcgttgaaga ttttaacgct acgctgccga
     3001 aatctcgtct gccgcaaaac accaccttaa ccgaagtgta attctctctt gccgggcatc
     3061 tgcccggcta tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca
     3121 taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt
     3181 agcgggtatt gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct
     3241 gaccgccgtc gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg
     3301 tgccatcgcc agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg
     3361 tttagctttt accctgcagg gaattatcgt caatattcag gcggtattct ggctgcaaaa
     3421 aggcaccgaa gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc
     3481 gctgctttca gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccggc
     3541 ggcatctcag ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcaatcct
     3601 ggtcggcatt ttgatgatct cgctgctgat tggtcagtcg ccagcgattc tgatcagcgg
     3661 tcttggtgca atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt
     3721 ggcaggtatt cagctttccg cgaacgatat gctgaaactg ggcgactggc tggaaatgcc
     3781 gaaatacggc gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa
     3841 ctgggacaat accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa
     3901 ctggagcggg atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt
     3961 caccagtatt cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt
     4021 aaagccttat ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc
     4081 tacggagtcg gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct
     4141 gaacgaatat ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca
     4201 actggctcca ggtgataacg gtttaccgct cgagatctac gcgtttacta acaccgtggt
     4261 gtggctggaa tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga
     4321 agagtttggt ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg
     4381 tgcatttaag cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt
     4441 ttgtcggatg tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg
     4501 aatacaatcc acaccggcag caggatcgct gacaagcgca tatcatccat cgtgcacatc
     4561 agcagcaaaa tcatgccgag gaaggcgatg caaagatagt tgccgaacgg atagagcagc
     4621 gccttaaact gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc
     4681 aggcagatca taatccagtt caacagcaac gttgctacca ccagcgtcat cagcagacca
     4741 aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa
     4801 agcatcagcg agttaatcgg cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca
     4861 ttaccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     4921 accgacagcg atgccaccag aatgacgaag ttcagcgcag aagctaccac attgctgtcg
     4981 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5041 agtgccagta aaaccaccag tgaaccgata taaaacagca ggatgcgata caccacctga
     5101 tttaccgctt ttggaatgct tttttccgga tcgcgcgctt cagcggctgt aatcccaatc
     5161 agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccag
     5221 ccggtggcga agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg
     5281 tgaccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta
     5341 atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag gttaacggcg
     5401 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     5461 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag
     5521 tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca
     5581 aatgaaccgg ataccggctc ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa
     5641 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     5701 gggccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata
     5761 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     5821 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     5881 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacgag gtatcctgct
     5941 ttaattccgt acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa
     6001 cagcgacagc aaaggtgcgg tgatcatgcc gccaatcatc ggggcggcaa tccggctcat
     6061 cacctctgaa ccagcccccg ttccccacag aatcggcagc agaccggcga taatcaccgc
     6121 caccgtcatc gctttcgggc gcacgcgcag gaccgcgccg tgatacaacg cctcatccag
     6181 cttctgctcg ctgaatgttt gtggattatt caacgacggc tcggcctcta tggcgtgacg
     6241 taaatacatc agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaagcc
     6301 ggtacccgtc gccacagaaa gatgaaagcc catccaccac agtaaccaga tgccgcccac
     6361 cagcgcaaac ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaatgccag
     6421 atacaacagc acgaagatga tcatcagtgt catcggcacc atgagtttca gcttatggtt
     6481 ggcgcgctcc agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg
     6541 cactttttca gctatcgctt tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg
     6601 ggcatcgata taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc
     6661 ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag
     6721 gatcggcaac tggcgcagtg cctgcggact atcgcgccag ctttgcggat aacgcagatt
     6781 aattggataa cgggcaatcc cttccaccct ttcgccaacc atcgccccgc ccaccgcaga
     6841 agtcacaaac aactgcacat ccgccaccgt cataccgtaa cgtgcggctt tttcacggtt
     6901 aatctcaacg ttgatatagc gcccaccttc cagccgctcg gcaagcgcag aagctacgcc
     6961 tggcaccgtt cgcgccactt cttcaatttg ctcagccatc gcgtcgatat ccgccagcac
     7021 agtgccggaa actttaatgc cgatggggct tttaatgccg gttgagagca tatcgatacg
     7081 gttacgaatt ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag
     7141 ttcctcaatg attttgtcca ttgtcatgcc tggccgccac tgatcctgtg gcttaagctg
     7201 gatggtcgtt tctaccatct ccagcggagc agaatcggtg gcggtttccg ctttcccggt
     7261 tttgccaaat acccgcgcca cttcaggtac gctcataatt agcttgtcgg ttttttgcag
     7321 catactcgcc gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc
     7381 gccttcattg atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt
     7441 cagcaccgaa agcgccgcca ccagcagcgt ggttttcggc cagtgcagta ctttcagcaa
     7501 cagcggatga taaacacgaa tcaaaaagcg attgagcgga ttactgcttt ccggcggaat
     7561 tttgccacgg atccagtaac ccatcaggat agggatcact acgatcgcca gcagcgccgc
     7621 acccgccatc gcatacgttt tggtgaacgc caacgggcca aacagacgcc cttcctgccc
     7681 ttccagggtg aagatcggga taaacgacaa cgtgataatc agcagactga taaacagcgc
     7741 cggccccact tcaacagacg catcggtgat cacctgccag cgcgttttat tatccagcgt
     7801 ggcgtcagga tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac
     7861 gatagcggca tcgaccatcg ccccgacggc aatcgcaatg ccacccagcg acataatatt
     7921 ggcattcagc ccctggaagg gcatgacaat aaaagcaata cacaacccca gcggcaacga
     7981 aataatcgcc accagcgccg agcgcacatg ccagagaaac agcgcgcaga ctaccgccac
     8041 cacaataaac tcttccagca acttgccgct gaggttgtcg atggcccggt caatgagctg
     8101 gctgcggtcg tatgttgtaa ctatctccac gccttccggc agactacttt tcagcgtttc
     8161 cagtttgtcc ttcacggcgg cgatcacttc tcgggcgttt ttgccggatc gcaggatcac
     8221 caccccgccc gccacttcgc cttcgccgtt taatgccccg gcgcatctcc gggccaatct
     8281 ggaccttcgc aacatcgcgc agataaacgg gcacgccatt ttcactggct tttaaaacga
     8341 tgtgattaaa gtcgtcgagc gtttgtagat agccgctggc gcgcaccata tattctgctt
     8401 ccgccagttc gattgacgaa ccgcccgctt cctggtttga agcatccagc gcgcttttta
     8461 cttcggcgag actgatgcca tactgcgcca ggcgctgagg atcgataacc acctgatact
     8521 ctttcaccac accgcctacc gacgccactt ccgcaacgtc aggaatggtt ttcagctcat
     8581 atttgagaaa ccagtcctgc aatgagcgta aatcggcaag atcgtgctta ccgctgcgat
     8641 ccaccagtgc atattcatag atccagccaa cacccgtggc atctggtccc agctcggcgc
     8701 tgactcccgc aggcagctta ccctgtacct ggttgaggta ctccagcacc cgcgaacgcg
     8761 cccagtacgg atcggtgcca tcttcgaaaa tgacatacac ataagagtcg ccaaactgcg
     8821 agaaaccacg cacagtcttc gcgccaggca ccgacaacat ggtggtggtt agcggataag
     8881 tcacctgatt ttcaacgatt tgcggtgcct ggccgggata gctggtttta ataatcacct
     8941 gcacatcgga gagatccggc aacgcatcca ctggcgtatt aatgatggtc caggtgcccc
     9001 agatgctcag aaacaacgcg cccatcagca ccaaaaaacg gttcgccacc gagcgacgaa
     9061 taatccattc aatcattggt tattccctca atgcgcatgg gtagcacttt cagagcgcat
     9121 ccgctccagt gcgccagaaa tattggcttc ggaatcaatc aggaacaggc cgccggaaac
     9181 caccttttca ccttccgcca gaccagagcg taatgcggtg acgccttgcg atgcctggaa
     9241 aacagcaacg cgtttcggta caaagcgccc gtcggcatca acggtaatca cccgctgttc
     9301 gttgccggta tcaatcagcg cctcgtcggc gttgtcgact tccagacgca gctgcagcgt
     9361 gcgggtcgcg gcatccacgc caggtagcag cgtccatttg cggatggtga gtgttttatc
     9421 cggtcgcgcc ggaacggtga gggtaaactg cgaggcatct ttcaccagcc aggcgatgga
     9481 ctccgggatc gcagcagtga cccacaccgg gtccataccc tgaattcgcg attacgcgca
     9541 atacccctga gaaccgccat gaacaagggt ttcatgattt gtgattttag tttagtacgc
     9601 aatttggtac acaacactat tttcacttca gcgggtagtc atcaaactca ccataacgag
     9661 catcgttgat gatgtatgtg atcaccacaa atatcacatc tggactagtt ccgtcttcat
     9721 gcgc
//
LOCUS       DAJTYF010000134         9708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTYF010000134 DAJTYF010000000
VERSION     DAJTYF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.134"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(329..673)
                     /locus_tag="OO590_003495"
     CDS             complement(329..673)
                     /locus_tag="OO590_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6638728.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="OO590_003496"
     CDS             805..2715
                     /locus_tag="OO590_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6638729.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQLGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="OO590_003497"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="OO590_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6638730.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="OO590_003498"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="OO590_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6638731.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRRQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="OO590_003499"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="OO590_003499"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6638732.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="OO590_003500"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="OO590_003500"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6638733.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNARQLRTRQLACLQLLAD
                     WRLRKARECDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="OO590_003501"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="OO590_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6638734.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="OO590_003502"
                     /pseudo
     CDS             complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="OO590_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(9210..>9708)
                     /locus_tag="OO590_003503"
     CDS             complement(9210..>9708)
                     /locus_tag="OO590_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6638735.1"
                     /translation="FAAKCGLSDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQS
                     QSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPE
                     IFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQD
                     VKKAV"
BASE COUNT         2370 a         2368 c         2559 g         2411 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtagcagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc taggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg gtcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 agacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgatgagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgccaggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagtg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaactgca
     7981 atctgataac tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 cggtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct
     9241 tgtgaaggag ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta
     9301 cgagtatctg ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata
     9361 ccttgcaaat cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca
     9421 aatcctttct ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg
     9481 acaattgcga tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct
     9541 tgattatttt tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga
     9601 cgccctgttg cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac
     9661 aggtagtttc tgataacccc atcagaaagt ccgcacttag ctgcaaac
//
LOCUS       DAJTYF010000135         9499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTYF010000135 DAJTYF010000000
VERSION     DAJTYF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.135"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..399)
                     /gene="ompG"
                     /locus_tag="OO590_003504"
     CDS             complement(<1..399)
                     /gene="ompG"
                     /locus_tag="OO590_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR6638736.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRW"
     gene            complement(444..1526)
                     /locus_tag="OO590_003505"
     CDS             complement(444..1526)
                     /locus_tag="OO590_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6638737.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(1540..2199)
                     /gene="pgmB"
                     /locus_tag="OO590_003506"
     CDS             complement(1540..2199)
                     /gene="pgmB"
                     /locus_tag="OO590_003506"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6638738.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(2196..4463)
                     /gene="ycjT"
                     /locus_tag="OO590_003507"
     CDS             complement(2196..4463)
                     /gene="ycjT"
                     /locus_tag="OO590_003507"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6638739.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     ARGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(4460..5066)
                     /locus_tag="OO590_003508"
                     /pseudo
     CDS             complement(4460..5066)
                     /locus_tag="OO590_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(5114..5860)
                     /locus_tag="OO590_003509"
                     /pseudo
     CDS             complement(5114..5860)
                     /locus_tag="OO590_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
     gene            complement(5891..6733)
                     /locus_tag="OO590_003510"
     CDS             complement(5891..6733)
                     /locus_tag="OO590_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR6638740.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVTWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(6720..7601)
                     /locus_tag="OO590_003511"
     CDS             complement(6720..7601)
                     /locus_tag="OO590_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6638741.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLLAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7622..8905)
                     /locus_tag="OO590_003512"
                     /pseudo
     CDS             complement(7622..8905)
                     /locus_tag="OO590_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(8919..>9499)
                     /gene="ycjM"
                     /locus_tag="OO590_003513"
     CDS             complement(8919..>9499)
                     /gene="ycjM"
                     /locus_tag="OO590_003513"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR6638742.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMDALSRRESSDEERCARFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         2267 a         2647 c         2324 g         2261 t
ORIGIN      
        1 ccagcgctgc atattcgccg tgtctttgcc cggttcatca acgtagtgat aaccataatt
       61 acggaaaccg ccggtcaggc cgaaactgaa atcatcgctt tcgaggaact gataatgcac
      121 ctccagctcc gggcgatcaa accacgttcc acgtttaccc gcgctataat ctaccggccc
      181 ttcctgataa taggccagag caattctcca cggcccgttg gcggcattaa aatagactga
      241 aggctccgcc agcccatcca tatcttcgcc ataaccctcg acgttttcta tttcgtacat
      301 cgcaccgata ttaaagtgcc agtcgttctt ttcctcggcc tgtgcgcagg ccattcccgc
      361 acacatcacc agtgcggtac agggtaataa ctttttcatt attgttcctt gtattatcct
      421 aggggaatgc cttccccctg tatttagcga attgctattt ccgtttctgc atcaaagaaa
      481 tgacatttcg tcatatcaaa atgaatagtg atattttctc ctgcatgata atcatttaac
      541 gcaccagcac ggaccactaa ctcgtgcccc ccaaccgtgg tgtagagcat aaattccgca
      601 ccggttaatt ctgccacgct aattttggcg gaaatgttat tttcctcttg cgcgtccgga
      661 tgaatatctt ccgggcgtat tcccatcacg atgggcttat gcaaactttc ctgtgttttc
      721 agaaccgcta atttctcttc gagaatggtt aatttaagcg tttccgtaac gaatttatcg
      781 ccatcgatcg tgccgcgaat aaaattcatc gctggtgatc caataaatcc ggcaacaaac
      841 atattcgcgg gttggttata aacggttttc ggcgcaccta cttgctgaac aatcccgtct
      901 ttcataatca caatccgcgt cgccatggtc atcgcttcgg tctgatcgtg ggtcacgtag
      961 atcatggtgg tgttcagttt ctgatgcagc ttgctgatct ctgcacgcat ttgtacgcgc
     1021 agcttggcat caaggttaga gagcggttca tccattaaaa acacgcccgc ttcgcgcaca
     1081 atcgcccgcc cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgt
     1141 ttcaggtact cacgcaggcc gagaatttgc gccgcccagt tcacccgctc atcaatcacc
     1201 tctttggcga ttttttgcat cttcaggcca aacgccatgt tgtcgtaaac cgtcatatgc
     1261 ggatacaacg cgtagttctg gaacaccatc gcgatattgc gtgctttggc tggaacgtca
     1321 ttcattcgtt tgccgtcgat caacagatcg ccgccgctga tctcctcaag cccggcaatc
     1381 atgcgcaggg tggtcgattt accgcagccc gacgggccga caaatacgat gaactcttta
     1441 tcggcaattt ccaggttgaa gtccttcacc acatgcacct ggttatcgta gattttttga
     1501 atatgttgta acgaaagctg agccatgttg attcctttgc tatacgtttt gccagaaggc
     1561 cgataaccgc gaccaggtca gggagtccgt tgagggcaac agtaattgcg ccccggttaa
     1621 gcccgcaccg atccccaccg agcgcatacc gctggcgtta atggcgtcaa tgcccgcctg
     1681 cgcatcttca atgccgatac atgcctgcgg cggcacgccc agcccggcac aggcagcgag
     1741 aaagatttcc ggatccggtt tcgagttttt aagttgggaa gcatccgcgc agaaggtgaa
     1801 aaactcgcgc agctccagcg ccgctaaaat cgtcggcgca ttcagggaga cggaagccag
     1861 ccctaccgga atctgctgtg cacggagctc tgccagcaaa ttgcgaatac cgggcagcac
     1921 agcattaacc gtcagctcgc gcagtgagtg gacgtaaagc agatttttgc gatacgccag
     1981 ttgcgcccgc tcctgcgggt taaagtcgcc ctctttgccc ccgtgttgca gaatgcgccg
     2041 cagagactca tcgcggctga tcccttttag agattcgtta aactgcgcat caatactgat
     2101 gccaatttca gcggcaatct gctgccacgc ctggaaatgc agatgcgcgg tatcggtgat
     2161 tacaccatcc agatcgaaaa ttaccccttg cagtttcatt catcctcctg atgcgtggta
     2221 gcggtcccat tgaagggcaa aataaaatca cccaaacaga aaacagattc ttcagccacg
     2281 gatataagct gaccgttcaa acgcagtgaa acgggcgcag aagttcgaat cgcaatacgc
     2341 tgcgcgtcga gagtgacctg taattcgcag ccctgccaga acagagggaa agacaactgt
     2401 tgccactgct caggtaacgc cggatttaga tgcaattcac cgtcacgcac gctcaccccg
     2461 gcaaaaccct gaatcgcccc cagccagata gcgccagttg cggcagcatg gataccatca
     2521 tcacaactat gcggatcagc accaagatcg atttcagtcc cctcgcgcca gaactgataa
     2581 ctttgggtca gcaggccaca gcgtgcagca acaatgccgt ggattgcttt acttaatgac
     2641 gagtcgtgaa tagtgcgcgg ttcataaaat tgcagattgg caagacacga tgccgatgag
     2701 aactgctctg gcagcatgta attgagcatc accacatcag cttgtttgag gatctgcatc
     2761 tcgttcactt ctgcgcgtga ataatccagc agaatggttt gcttccccgc cgccgctttg
     2821 tatttcgcca gattaatcgc cggcttagtc ataaacgaat catcctgcgg caaaacaccg
     2881 tcgggctgaa tttctggcag ccgcagttct ttaaggaaca tttcggcacg atgaataaac
     2941 gcgtcgtcac tacagccaaa ctggcgggca atactcagcg cctgctggac gttgtaatac
     3001 accatatagc tggtgaaggc gttattattg acatgttcgg tatattcgtc tggcccaata
     3061 acatcatgaa tttccagacg gtcgttaacc ctcaccgcgc ggctaatcca gaactttgca
     3121 gtttccagaa gtagcgccat gccttcatgc gcaatgaaac tttcatcccc cgtggtctgc
     3181 cagtattgaa taaccgccca ggcgatatcg gccaccagat gatgttccgc ctgcgccgag
     3241 gccacttttt gccgcagccc ggtgcgaatg ttaatggcgg caaattccgg cgtctcttct
     3301 tcgccgctgc gcgcgctttc ccacggaaat agcgcgccct gccagccgtt gcgtcgcgct
     3361 ttctcctgcg cgcctggcaa gttgtgccag cgataacgca gtaaacttcg ggcaaccgtc
     3421 ggatcgctaa acagatggaa cggcagcaaa aatacttctg tatcccagaa aacgtggcct
     3481 ttgtagcctt cgccggttaa gccttttgcc gcaatactgc tgcgctcgcc gtgagccggc
     3541 gtcatgatgc gcagatgata aagcgcataa tctaacgctt gctgatcgtg cgcatcgccg
     3601 ccatttaccg tgatacgacg tttctgccac cattgacgcc agttttctgt tgatgctgca
     3661 agatgttggt cgtaactctg ttgcgcgcac atttcaagct ggcgaagcga cacgctgccc
     3721 cactcgtcta aagcagcttg cctgtcatcc cgccagtcga tccacaccag tttttgcaac
     3781 gtcactgtct cgcctgcatg aagctgcgca ctggtatgtt gcagtaaacg gcgctctttg
     3841 gcggtatagc attgctgcac atcaccgctc acctggcagc aacaactgat ggccacatca
     3901 ctgcgcccat cctgggtggt gtagatcccc tgcatcaaat gctgaccaaa cacccgcacc
     3961 tgggtttcgt cgagatgctg gcgaccgtgg ttggtttgcg tggcgtcaat gcctgttgaa
     4021 atcagcactg acgcgtcggc gtccagtggc ataatagtga tttccagcgc aatgagcggc
     4081 agttggtctg ccgaaacaaa gcgacggctg gcgatggtgt aacctgcgcc gttgctggta
     4141 cgccagacaa cgctacggcg taattctcca ctggcaaagt caagttcacg ctgccaggct
     4201 tcgcgggata acgagaaaac ctcaccattt atggcaatct ccatccccaa gatatcaggc
     4261 agattcacca gttcgttgat ttcacctttt cccgcccgat gatacagccc cgccagatac
     4321 atccctcgcg cctggcgcgt gtaatcttct tcatggctgg cgcgaagccc aagatagccg
     4381 ttcccctgcg ccatcagcga tgcatacttg ttcagggtat gctggctgaa atggggttct
     4441 gataacgtta ctggcctggt cataattcca cacgcgtccc tgtttctgcg gattgatata
     4501 acgccgccac cagttgctgg atgatgtacc cctgctcggc gtcggctatc atcacgggct
     4561 tgccctgtac gtgattgata aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca
     4621 tttcccgttg catcagcgtc attaattcac cgttgttatc ggtgtagata tgtgctggaa
     4681 acagcgtcgc accggcttta tcaccacaaa agctgacgtt cataatcaac tgttcgcgga
     4741 tgttgagtgc aaatgacgtt tccagccaca gaatgccgcc gttatgaaat tcaatggtgc
     4801 caaacagcga atcttcgacg ctgtaagttg ccggatccca ctcgccaaat tgaccacagc
     4861 tcttttgcgt gccgatcttt tgaaaactat gcgcattcac gcttttcacc gccggaaagc
     4921 ccagcacata catcgcagca tccagcatat gaatgccgat gtcgatccag ttctttattg
     4981 gtaaagacac cccagccggg aacgccgcag cgacgcaggg cgcgggcggt ggtaacgtaa
     5041 atctctccca aaacgccgtt ggtcacctgt tcacgcagct gttgcgtatc gagcgcaaac
     5101 gatggtgaaa gtcgtaggcc aggccgcgca gcgccgattg cagcgcgtcg gcgtaaccgc
     5161 tggtttcgat aatcacatcg gccccctgct tgccggtcag cgttttgatc tctttgccta
     5221 cgtcagtgcc aatggggtta aggcagaaat cagcaccgtg gcgacgggca atatcacagc
     5281 gatgggcgat aggatcgacg ccaattacca ccgatgcgcc agcgcgtttc gccagttgga
     5341 tggcaatttg accgatcgcg ccaagcccta ccaccacgac aaaatcccct acgcgtacgt
     5401 tggcatcgcg cacgccgctc atggcaaact gcgccgggtc gtaacagacg gcgtttttcc
     5461 aggagctgcc ttgcggcatt ttgcgcagct tgtagttatt cactgcgtta atgatgaccg
     5521 tctcggagag cgggccgtag ccgcatacgc tgtcgcccac cgcgtagtct gtaacgtcgc
     5581 tgccgcactc gataatgtcg ccaaccacca tgttgccaag ctggaatttg ccaaactcaa
     5641 tgccgcgcgg cgcatctgcc ggacgcgggg tgaacatctg ccactcgccg ttaaagtctt
     5701 catcgataaa cgggctggcg gcgcggaagt cgaccacttc cgttccgtgt ttcggtgcgc
     5761 cgaaacgcac gcggatcttc acttcattgg ctaaaatcgc ccgatcttca tactcaacca
     5821 gcgcagcaac acgcggtgct gtggctacta actttttcat gtaaaactcc ttgtaaagaa
     5881 actggccgct ttagcccttc acgccaccgg cggtcaaacc actcttaata aaacgttccg
     5941 aaagcgcata cataatcacc accggcaatg cggtcaccag cgaggccgcc atcatccgtc
     6001 cccagatgta atcaggcgta ctgaacagcg cgttcaggcc cactggtaag gtgaaattgc
     6061 tggcgctgga gaggaaaatc gacgcaaaca gatagtcgtt ccacgtcacc ataaagcagt
     6121 agacgaacac cgatatcagc ccggacatcg ccaggggcac ggtgatacgg aagatgattt
     6181 gcagacggtt gaggccatcc atcatcgccg cttcttcgat ctcatcaggg atggtgtcga
     6241 agtaactttt cagcataaac acggcggtgg gcagggtttg cgtcaccatg gtaatgatca
     6301 gcgccatctc ggtgtcgtaa ataccaagcg cggtgatgat tttgaacaga ggcaccacca
     6361 gcaaaatgcc agagaacata tacaccgtgt aaaagctggc gttgatggtc atccggcctt
     6421 taaagcgcag gcgggaaagc gcgtaagctc cgagaatgcc gagaaatacc gccaccacgg
     6481 aggaaaccac cgacaccacc atgctgttac ggaagtagtc gacaaacggg aaaatcatcg
     6541 ggttaaaaat gtcgacgtaa tgctccagcg tccactgttg cggcagcagc gtaggatgca
     6601 gcgatatcgc ctctttcgcg cttttgaacg aggtcatcag catcacaaaa aacggaaaca
     6661 gcgtgatgat gagaaacagc gccagcccgc agtaaaaacc gatacgactg agcgtgcgtt
     6721 tatttgttgc cattgaggtt cacccgtttc ctggtcagca gaatgacagc gaaaataatg
     6781 atgaagagca ccaccgagat cgccgccgct ttccctaagt cattaaaggc aaaggcggtt
     6841 ttgtagagat agacaccaag aatatcgacc ttggttgtca gcagataaac atcagcgaac
     6901 atgtagaaca tccagatggt gcgcagggtc accaccgtcg ccaagaccgg cataattgcg
     6961 ggcagcgtga cgatgcgaaa ccgctgccag gcattagcgc catccatctc tgccgcttcg
     7021 tacagcgatt tgtcgatggt ctgcaaaatc gccagaaacg agataaaggc atacgggaag
     7081 tagcgccaga tggcgagcag caccaccagc acaaagctac tgcccggatt atcaaaccac
     7141 agcggtgcct gctcatagag atgcagaaga tcgacgccga ggtagttcac aatgccgtag
     7201 ccgttgttga acatgtattt ccaggcaaac accagcgaaa tggacggcgt tacgtaggag
     7261 aggatcacca gcgaacgcgc ggttttgcgc aggcggaact cgcggttgaa aaacatcgcc
     7321 acggccagcc cgagcacggt gctgcccgcc accaccagcg ccgtatacca gaccgtcatc
     7381 cacagcgagt gccagaagcc gggatcggag agaatgcgca catagttact caccccaaca
     7441 aacgttgact cgatgttggg attgagcggc agacgtaaaa aactgatttc gatattcgac
     7501 accatcggcc acgccaccag accgcccagc agtaataagc tgggcgcgag aagcagcagc
     7561 gcaaacggca tatcggaacg acctgaaaaa agcctgttca ttgcggattc ctgtcctggg
     7621 tttagtgctg ttcgatcagt tcatccagct ttttctggct cgcttgcagc gtgccaggga
     7681 gatcggcttt acccacggtg acgttatgca ccatggagct caccacacca gaacccgtca
     7741 catcgcccat gcgggtaaag tttttatccc ctactgcgcc aaagacctga atatttggca
     7801 gttcaccgat tagctgattc ggtagttcac ccagcgcctt aataacgtcg ttgtctttcc
     7861 aagtggcggt agtgacaacc gctttattca ccggcagcgc agcacctggc gacatcatca
     7921 cccagtcggc aatgttgtcc gcctgctcca taaaggtgac aaatttttct gctgcttcgg
     7981 tcttttgtcc ggcggtaatg gtcagcgagg tcaacatgcc gtagaccgca gagtttttct
     8041 cggttggcac cacgaaaccg acgtttttcg ggtcgccttc tttaatcaca gccggaagga
     8101 tataggtgga gtaaatcgcc atcggcgcgg tgccgttcat aaaggcgtct ttcacttcca
     8161 tgatgtcgtt agaacccggc atggtgttgg cagcaaggtc gcggtaatag gtcagcgcct
     8221 gcatcatctc tggtgtatca agggtgattt tgccttcggc gttaaagacg ttggcctagt
     8281 tggataacgc aaactgggag aaggattgtt ccgtcagcac gctttctgcc gtaggcagcg
     8341 caataccgta ttttttattc gccgggtcat tcagtttctg tgcaacgtcc agcagctgtt
     8401 gccagttttt cggctcctcc agccccgctt ttgccagcac atctttgcga taccagatgc
     8461 cgccaatcca ggcgctgaca ggaacaccgg tccatgcgct accatcttcg gtacgcacaa
     8521 tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt gctgatgacc gtggcaaccg
     8581 ctttgcgatc gataagctgc tctttatcca taactttggc gtagtcatgg ctggtttcga
     8641 tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac tttggtgtta taagcgtctt
     8701 cttccacggg cacttgttta acggtgatac ctgggttttc tttttcaaaa cgggcaatca
     8761 atttgctgat aactgcctga cgctcctgct ccaccgaaga atgcataaat tcgatggata
     8821 catgggtttt attttcttct ttacagcctg aaatcagggc gcaggaaacc agtgctgata
     8881 acagcacgat ttttgattta atcatgagat gttccttttt atttaatcca cataacctgc
     8941 caagggcgca gcgttatttc attacccaat atatcaactt cactaatcag atcccgacca
     9001 tgcagatcag taatatttac atgctgaata tttttactga cattaaacaa tccagtcaga
     9061 caattaccat cagcgttact tctttgaata cgcattacgg atgaattaac cgtatcgatg
     9121 gtaaaattat tatccggatg aaactcgtta tggctgcgac gaagtgtaat taaacgcgac
     9181 aactcatgat ataccgcatg ccttaatgta gcttcatcgt tcagttctcg tgttatctct
     9241 tcactgtgat attttttacg gttaatcgca cggttatatc cgagtttttc gacacctgcg
     9301 taatcattac gcgagccaag aatactttga atatatatcg cgggtacacc ggggaaactt
     9361 aacaaaatcg catgggcaag gataaacctg gcgcaacgtt cttcatcgct actctcgcgg
     9421 cggcttaacg catccatata ggtgacattc atctcatagg gactgcgcgt gccgtcggga
     9481 ttatttttcc agtttacta
//
LOCUS       DAJTYF010000136         9040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTYF010000136 DAJTYF010000000
VERSION     DAJTYF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.136"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO590_003514"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO590_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6638743.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1860)
                     /gene="phoA"
                     /locus_tag="OO590_003515"
                     /pseudo
     CDS             complement(446..1860)
                     /gene="phoA"
                     /locus_tag="OO590_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO590_003516"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO590_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6638744.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2403..2606)
                     /locus_tag="OO590_003517"
     CDS             complement(2403..2606)
                     /locus_tag="OO590_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638745.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="OO590_003518"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="OO590_003518"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6638746.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="OO590_003519"
     CDS             complement(3802..4014)
                     /locus_tag="OO590_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6638747.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="OO590_003520"
     CDS             4274..4582
                     /locus_tag="OO590_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6638748.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO590_003521"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO590_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6638749.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO590_003522"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO590_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6638750.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="OO590_003523"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="OO590_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6638751.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="OO590_003524"
     CDS             complement(8478..8981)
                     /locus_tag="OO590_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6638752.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2240 a         2314 c         2149 g         2337 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctattgaaaa gattaacttt ttactgacga ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagtaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc aacctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gctcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcagcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagaacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc agtggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctac tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattatt tagattaaat
//
LOCUS       DAJTYF010000137         9010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTYF010000137 DAJTYF010000000
VERSION     DAJTYF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.137"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(95..1471)
                     /gene="trkA"
                     /locus_tag="OO590_003525"
     CDS             complement(95..1471)
                     /gene="trkA"
                     /locus_tag="OO590_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6638753.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1493..2782)
                     /gene="rsmB"
                     /locus_tag="OO590_003526"
     CDS             complement(1493..2782)
                     /gene="rsmB"
                     /locus_tag="OO590_003526"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6638754.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2828..3775)
                     /gene="fmt"
                     /locus_tag="OO590_003527"
     CDS             complement(2828..3775)
                     /gene="fmt"
                     /locus_tag="OO590_003527"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6638755.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3790..4299)
                     /gene="def"
                     /locus_tag="OO590_003528"
     CDS             complement(3790..4299)
                     /gene="def"
                     /locus_tag="OO590_003528"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6638756.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4447..5571
                     /gene="dprA"
                     /locus_tag="OO590_003529"
     CDS             4447..5571
                     /gene="dprA"
                     /locus_tag="OO590_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6638757.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5543..6016
                     /locus_tag="OO590_003530"
     CDS             5543..6016
                     /locus_tag="OO590_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6638758.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6045..6587
                     /locus_tag="OO590_003531"
     CDS             6045..6587
                     /locus_tag="OO590_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR6638759.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6592..7164
                     /gene="tsaC"
                     /locus_tag="OO590_003532"
     CDS             6592..7164
                     /gene="tsaC"
                     /locus_tag="OO590_003532"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6638760.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7169..7987
                     /gene="aroE"
                     /locus_tag="OO590_003533"
     CDS             7169..7987
                     /gene="aroE"
                     /locus_tag="OO590_003533"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6638761.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7984..8241
                     /locus_tag="OO590_003534"
     CDS             7984..8241
                     /locus_tag="OO590_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6638762.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8217..8771)
                     /locus_tag="OO590_003535"
     CDS             complement(8217..8771)
                     /locus_tag="OO590_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6638763.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2255 a         2189 c         2248 g         2318 t
ORIGIN      
        1 aacaaatgtt aagccatttt cctgccagga atgaatttaa ttaatccttg ataatcaaag
       61 ggaaataata aggcgtcatt agacgcctta ttaattacaa gaagaaaggg cttggctgga
      121 agagtctttc gacgtcggta ataaactttt tatctgtgag gaacataatt acgtgatcgc
      181 cttgctcaat gcgcagattg tcattggcaa tcatcacgtc gtttccacgt accaccgctc
      241 caataatcgt tcctggcggt agcttgattt cgtcaatgac tctgccgaca acgcgtgagg
      301 tgctttcatc accgtgagca acggcttcaa tagcttctgc tacgccgcgg cgcaatgagg
      361 aaacaccaac aatatctgct tttcgcacat ggctaagcaa cgcagaaata gttgcttgtt
      421 gtggtgaaat tgcaatatcg atcacgctcc cctgaaccag atccacataa gcgcgacgct
      481 ggatcaatac catcaccttt ttcgcaccca tacgtttggc aagcatggcg gacatgatat
      541 tggcctcgtc atcgttggtg acggcaataa acagatcaac ttgatcgata tgttcttcgg
      601 ccagcagttc ttgatccgac gcatcaccaa aaaagacgat cgtattctgc aacttttccg
      661 ccagttcggc agcgcgctgc tgattacgtt cgatgagttt aacgctgtaa tctttttcca
      721 gacgacgcgc cagccctgca ccgatattac cgccaccaac cagcatgatc cgtttatacg
      781 gtttttccag tcgctgtaat tcactcatca ccgcgcggat atgctgtgaa gcggcaataa
      841 agaacacttc atcaccagct tcaacaatgg tcgaaccttg tggacgaatg gggcgatcgt
      901 ggcggaaaat tgctgccaca cgagtatcga tatgtggcat atgttcgcgc atggtcgaaa
      961 gtgcattacc aatcagcggg ccgccataat aggctttaac tacagcgagg ctgactttac
     1021 cctcagcgaa gttcaccacc tgcaatgcgc ctggatactc aatcagtcgg taaatattat
     1081 cgataaccaa ctgctctggt gcgatcagat gatcaatcgg tacagcatct gaatgaaata
     1141 gcttatcggc atcgcgtacg tagtctggtg agcggatgcg agcgatgcga ttaggggtgt
     1201 tgaaaagtga gtaggctacc tggcaggcaa ccatattggt ttcatctgaa ctggttacag
     1261 caaccagcat atcggcgtcg tcggcacctg cctcccgcaa tacgcgtgga tgagagccat
     1321 gcccctgcac gactcgcagg tcaaatttat cctgcaaggt ccgcagacgc tcaccgttgg
     1381 tatcgacaac agtaatatcg ttgttctcgc caaccaggtt ttccgccagt gtgccgccaa
     1441 cctggccggc acccagaatg ataattttca tcagtcgcga cccgttatct catcactttt
     1501 tgattagctt agcgtaaaag aagccgtcgc cctcttcggc accaggctga ttttgtttac
     1561 ccggttgctc tggtgttcct gtttcgcaaa gttcggcatc agcggtacgt tgcaaaaagg
     1621 ctttaatctg caggctattc tcttccggta acaccgaaca ggtggcatag accagggttc
     1681 caccggattt taaatgcggc caaatggcgt cgagaatttc agactgcaat tgcgcgagtt
     1741 ccgggatatc gcgatcgcga cgtaaccatt taatatctgg atggcgacga atcacccccg
     1801 ttgctgaaca aggcgcatct aataaaatgc gatcaaactg ttgctcgcca caccattggg
     1861 aagggtaacg gccatcacct tgtttcacgg ttgccttcat accaaggcgt tttaaattgt
     1921 cgtaaacgcg agagaggcgc tgttcgtcaa tatcaaccgc aacaacctgc gcttctggtg
     1981 ccacctcaag gatatgcgtt gttttaccgc cgggggcggc acaaagatcc aagatgtgtt
     2041 caccgttttg tggcgcaagc caggtcatgc aaccttgtgc tgatgcatcc tgaacggtaa
     2101 cccatccgtc ttcaaaacca ggtagcgcat gaacaggtgc aggtgtttcc agccgtacag
     2161 catcagggta atccgcatgc gggaaacctt tcattcctgc ttcatccagc aatgcaagcc
     2221 agctgtcgcg ggaatgatgc gtacgattaa tacgcagcca cattggcgga cgctggttat
     2281 tggcttcggc gatggattgc cactgctctg gatacgcttt ttgcagacgc ttcagcaacc
     2341 aggaaggatg cagataacgt gcatcacggg tattaaactc ggctaataac tcttcttgcc
     2401 gacgctggaa ctggcgtaat acaccgttaa tcaacccttt aagttgcgga cgcttaattg
     2461 cgatagcgcc ttcaaccgtt tcagccagcg cagcatgagg tggaatgcgg gtataaagca
     2521 gttgatacaa accaaccata atcaggtaat gcacagtgcg ctgtttgccg gtcatcggac
     2581 gggccattaa cttattaatc agccagtcta actgcgaaag cgtacgcagt acgccaaagc
     2641 acaactcttg aagaagtgct ttgtctttat cggaaacttt ttgctggagc ggtggcagaa
     2701 tgttgcttaa tgattgccct tgctcgacga cttgttcaac ggcctgggcc gccatgctac
     2761 gtaaattacg ttgttttttc ataagtataa aaatgctcgg caagaccggg cttagaagag
     2821 tggactatca gaccagacgg ttgcccggaa caaaccattc ccgacgagag ttcaggaggt
     2881 cttgcgcgct catcgctttc ttacccgcag gttgtaacga gagcaggttc aggatgccat
     2941 caccagtcgc aacctgaatg ccttgtttgt tggcttcaag gatcgttcct ggtgcagcgt
     3001 tggttgccgt atcaatgacc gacgctttcc agactttaac cggctgtcct tcaatttcca
     3061 gccagctcat tggccatggg ttgaaagcgc gaatgcagcg ttcaagctgt gctgccgaaa
     3121 gtgaccagtc aatacgcgct tcttctttac tcaacttctc ggcgtaagtg acaagagttt
     3181 cgtcctgaac ttctggtttc gccgtgccgt ctgccaattg tttcaacgtg gtgataagcc
     3241 cttgtgggcc aagctctgcc agcttgtcgt acagcgtacc actggtatct tctgcagtaa
     3301 tcgggcagga gagcttatag agcatgtcac cggtgtctaa accaacatcc atttgcataa
     3361 tggttacacc agtttctgca tcacccgccc atagtgagcg ttggattggt gcagcaccgc
     3421 gccagcgtgg cagcagtgaa ccatgaacgt tgatacagcc aagacgcggc atctccagca
     3481 cagctttagg cagaattaaa ccataggcga cgacgaccat aacatcagcc tgcaaatcgg
     3541 cgaccagttg ctggttttct tgtggacgca gggaaacagg ttgaaaaacg ggcagacctt
     3601 cttcctcagc cagaacttta atcgggctgg gcatcagttt tttaccgcgt cctgccggtc
     3661 ggtctggctg ggtgaacacg ccaacgacgt tatgaccaga agacaacagc gcgtcgagat
     3721 gacgcgctgc aaagtcaggt gtacccgcaa aaataatacg tagtgattct gacacgttag
     3781 ttcttatcct taagcccggg ctttcagacg atccagtttt tcaactttct gacgaattcg
     3841 ttgttgtttc agcggtgaca gataatccat aaacagtttg ccaaccaggt gatccatctc
     3901 atgctgaata cagatagcta acagaccctc tgcttccagt tcaaatggtt taccgtcgcg
     3961 gtcaagggcg cgaattttaa ctttctctgc gcgcggcact aaagcacgtt gttcagggat
     4021 cgacaggcaa ccttcttcaa tgcctgtttc gccgcttttt tctaaaagct ccggattgat
     4081 taacaccagc cgttcgtcac ggttttccga aacatcaata acaatgatac gttgatggat
     4141 atcaacctgg gttgccgcca ggccaatacc ttcttctgcg tacatcgtct cgaacatatc
     4201 atcgacgata cgctgaattt ctgcattcac ttcttctacc ggtttagcaa ctttgcgaag
     4261 ccgctcgtcc ggaatatgta acacttgcaa aactgacata aatctccaga gatgtgttca
     4321 ggagttagaa agattatttc ttctattcta gacaaatccc cctctgattg acagcatcac
     4381 ttgattgaca gcatcactga ccaatcgcaa agattgctaa ggcttgctta tgacagggag
     4441 ataaggatgg tcgatacaga aatttggctg cgtttaatga gcatcagcag cttgtacggc
     4501 gatgatatgg tccgtatcgc tcactggctg gcaaaacagt cgcatattga tgcggttgta
     4561 ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt tccacgaaag
     4621 agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat ccctgcggac
     4681 agcgaatttt atcctcctca acttcaggcg acgacagatt accccggcgc actgtttgtt
     4741 gaaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag tcgggcgcat
     4801 tcatggtatg gcgagcgatg ggggcgatta ttttgcgaaa ctctggcgac gtgtggagtg
     4861 acaattacga gtggactggc gcgtggaatc gatggtgtgg tgcataaagc ggccttacag
     4921 gtaaatggcg tcagcattgc tgtattgggg aatggactta atatcattca tccgcgtcgc
     4981 catgcccgac tggctgccag tctgtttgaa cagggtggcg ctctcgtctc ggaatttccc
     5041 ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat cagtggtcta
     5101 agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctggt gacagcacgt
     5161 tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg gaatccggga
     5221 agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga accggaagaa
     5281 attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga aaattcattt
     5341 tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc taacgtagga
     5401 gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt gccagaggta
     5461 gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc cggcggctat
     5521 gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg aaacctatat
     5581 tcacactgaa gctgagttgc gtgtggatca agacaaactt gaacaggatc ttaccgacgc
     5641 aggttttgag cgagaagata tctacaatgc cctgctatgg ctggaaaaac ttgctgatta
     5701 tcaggaaggg ttggcagaac cgatgcaact ggcctcggat cctctctcca tgcgtattta
     5761 tacaccggaa gagtgtgaac gactggatgc cagctgccgt gggtttctgc ttttccttga
     5821 gcagattcag gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag tgctcgcgct
     5881 ggataacgca gagttcgaac tggatgatct gaaatgggtg atcctgatgg tgttgttcaa
     5941 catcccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg aagtgaatga
     6001 aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggca aaatcagcac
     6061 tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg gttattcgat
     6121 ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac tacgtccgtc
     6181 ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag gtttgccccg
     6241 tatgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt ggttgcagta
     6301 actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca gcaattacat
     6361 gcccccaatg tcggacgggc catctggtcc agcgccgctc ccgttatggc aaaacatttc
     6421 actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaacca atagctggag
     6481 aatgccctga gtgtcattat ccgctactca tcgaaaagaa aaccgcgcag ggtgtaaaac
     6541 acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa cgtgaataat
     6601 aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaaa acgtgtcatc
     6661 gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga aacagcagtg
     6721 atgagactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt aatcgcggca
     6781 aattacgagc agcttaaacc ctatattgat gacaccatgt tgactgacgt gcagcgtgaa
     6841 accatttttt cctgctggcc aggtcctgtc acctttgtct ttcccgcgcc tgcgacaaca
     6901 ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga ccatccgttg
     6961 gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc caacttgagt
     7021 ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc ggcgttcccg
     7081 gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga tgccctgacg
     7141 ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg gtaatccgat
     7201 agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga atattgaaca
     7261 tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga acgctttctt
     7321 tcgtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg cttttgccag
     7381 agcggatgag cttactgaac gggcagcgtt ggctggtgca gttaataccc tcaagcggtt
     7441 agaagatgga cgcctgctgg gtgacaatac cgatggtgta ggcttgttaa gcgatctgga
     7501 acgtctgtct tttatccgcc ctggtttacg tattctgctt atcggcgctg gtggagcatc
     7561 tcgcggcgta ctactgccac ttctttccct ggactgtgcg gtgacaatca ctaatcggac
     7621 ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta ttcaggcgtt
     7681 gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa catccagtgg
     7741 catcagtggt gatattccgg cgatcccgtc atcgctcatt catccaggca tttattgcta
     7801 tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc agcgaggctc
     7861 aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg cctttcttct
     7921 ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg aggaattgtc
     7981 cgcgtgaatc aggccatcca gtttccggac agggaagagt gggacgagaa taaaaaatgt
     8041 gtatgttttc ccgctctcgt gaatggtatg caactgacat gcgcgatctc tggcgagagt
     8101 ctggcgtatc gctttactgg agatacgcca gaacagtggt tagcgagttt tcgtcagcat
     8161 cgctgggacc tggaagaaga agcggaaaac ttaattcagg aacaaagtga agatgatcaa
     8221 ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta attattcgcg
     8281 gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt gacggggcta
     8341 ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact acccgcacca
     8401 atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat cccaaccaaa
     8461 actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt gacatcttcg
     8521 ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac atgcaacata
     8581 ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc tccacgaatc
     8641 acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat cacgacactg
     8701 ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta tgggcgtaaa
     8761 acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg ggcattattg
     8821 gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat tgcagcaaaa
     8881 agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca aaatattggg
     8941 atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacagg atggtcggaa
     9001 caacgaagag
//
LOCUS       DAJTYF010000138         8987 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTYF010000138 DAJTYF010000000
VERSION     DAJTYF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.138"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            40..2926
                     /locus_tag="OO590_003536"
                     /pseudo
     CDS             40..2926
                     /locus_tag="OO590_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(3036..3884)
                     /gene="alkA"
                     /locus_tag="OO590_003537"
     CDS             complement(3036..3884)
                     /gene="alkA"
                     /locus_tag="OO590_003537"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR6638764.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            4018..5370
                     /gene="yegD"
                     /locus_tag="OO590_003538"
     CDS             4018..5370
                     /gene="yegD"
                     /locus_tag="OO590_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6638765.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5382..7322)
                     /gene="yegI"
                     /locus_tag="OO590_003539"
                     /pseudo
     CDS             complement(5382..7322)
                     /gene="yegI"
                     /locus_tag="OO590_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7319..8080)
                     /locus_tag="OO590_003540"
     CDS             complement(7319..8080)
                     /locus_tag="OO590_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCR6638766.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8077..8736)
                     /locus_tag="OO590_003541"
     CDS             complement(8077..8736)
                     /locus_tag="OO590_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6638767.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2194 a         2381 c         2343 g         2069 t
ORIGIN      
        1 taacacccca ttataagggg tacgaataaa gtaacggggc tggttgtcct gctgacgccc
       61 ggagacgatc ctctcagggc atttttgata tgggtactgt cagaatccat cggcgctctg
      121 gcactggtgc cgctgggatt gttatttaaa ccacactatc tgctgcgcca tcgcaaccca
      181 cggttgcttt ttgagtcgct gctcacgtta gccatcacac tgacgttaag ctggctttcg
      241 atgctgtatc tgccgtggcc ttttactttc attattgtgc tgttgatgtg gagcgccgtg
      301 cgcctgccac gaatggaagc ctttttgatc ttccttacca cggtgatgat ggtgtcgctg
      361 atgatggccg cggatccctc cctgcttgct acgccgcgta cgtacctgat gagccatatg
      421 ccgtggctac cgtttttgct gatcctgctg cccgccaaca tcatgaccat ggtgatgtat
      481 gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa cccgctttcg gaacgcgatg
      541 gaatattccg ctatcggtat ggcgttagtg ggcaccgagg gacaatggct gcaatccaac
      601 aaagcactct gccagtttct cggttacagt caggaagagc tgtgcggact cacctttcag
      661 caactgacct ggccggagga tctcaataaa gatctccaac aggttgaaaa gctgataagc
      721 ggtgaaataa acacctattc aatggaaaaa cgctactaca accgcaatgg cgatgttgtc
      781 tgggcgttgc ttgccgtctc actggtgcgc cacacggatg gcacgccgct ctattttatc
      841 gctcagattg aagacattaa cgagctaaaa cgcaccgaac aggtgaatca gcaactgatg
      901 gagcgcatca cgctggctaa cgaagcgggc gggattggca tctgggagtg ggagctgaag
      961 ccgaatattt ttagctggga taagcggatg ttcgagctgt atgaaattcc tccgcatatc
     1021 aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg aagatcgcca gcatgccgaa
     1081 aaagtgattc gttgcaatca cgctcgccct ttaagctgga atttcgcatt gccgtgaaag
     1141 atggcatccg ccatatccgc gccctcgcca accgggtact gaataaagaa ggcgaagtcg
     1201 aacgcctgct cggcattaat atggatatga ccgaggttaa acagcttaac gaggcattgt
     1261 ttcaggaaaa agagcgcctg cacattacgc tggattccat cggtgaagcc gtggtctgta
     1321 ttgatatggc gatgaaaatt acctttatga atccagcggc ggagaagatg agcggctgga
     1381 cgcaggaaga agcattaggt gttccgctcc tgacggtgtt gcatattact tttggcgaca
     1441 acggaccatt aatggagaac atttacagtg ccgacacctc acgttccgcg attgaacaag
     1501 atgtggtgtt gcactgccgg aacggcggca gttacgacgt gcattacagt attacgccgt
     1561 taagtactct ggacggcagc aatataggct ctgtactggt gattcaggac gtcaccgaat
     1621 cacgcaaaat gctgcgccag ctgagctaca gcgcctccca tgatgcactg acgcatctcg
     1681 ccaatcgcgc caattttgag aagcaactgc gtatcctgct gcaaacggta aacagtacac
     1741 atcagcgaca tgccctggtg tttatcgatc ttgatcgctt taaagcggtg aatgacagcg
     1801 ccgggcatgc ggcgggtgac gctttactgc gcgaactggc gtcgttgatg ctgagtatgc
     1861 tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga atttggtcta ctgttgccag
     1921 actgtaatat cgaaagtgcg cgttttatcg ctacacgcat tatcagtgcc gtgaatgatt
     1981 atcactttat atgggaaggc cgtgtgcatc gggtaggtgc cagtgccggg attaccttga
     2041 ttgatgacaa caatcatcag gcggcagaag tgatgtcgca ggctgatatc gcctgttatg
     2101 cctccaaaaa tggtggacgg ggccgggtga cggttacgaa ccacagcaag ctgccgcaca
     2161 tagcgaacgg gcggcgatgt cgcttgatga acagtggcgg atgattaaag agaatcagtt
     2221 gatgatgatc gcccacggtg tcgcttcgcc gcggatcccg gaagcgcgta atttgtggct
     2281 gatttcactt aagctctgga gttgcgaagg cgagattatt gatgaacaaa aatttcgtcg
     2341 tagcttcagc gatccggcac ttagccatgc tcttgaccga cgggtattcc acgaattttt
     2401 ccagcaggcc gcaaaagcgg ttgccagtaa aggcataagc atcgccctcc ccctttccgt
     2461 tgccggtttg agtagcgcca cgctggtgaa tgatctgctt gagcagctgg aaaatagccc
     2521 tctaccacca cggttattac atctgattat tccggctgaa gcgattttag atcacgcaga
     2581 aagcgtgcaa aaactgcggc tggcgggatg tcggatagtg ctcagccagg tgggccgcga
     2641 tctgcaaatc ttcaactcgc tgaaagcgaa tatggcagat tacctgctac ttgatggtga
     2701 gttatgcgcc aacgtgcagg gtaatttgat ggatgagatg ctgattacga ttattcaggg
     2761 gcacgctcag cgactcggga tgaaaaccat cgccgggcca gtcgttttac ccttagtgat
     2821 ggatacgctt tctggcatcg gcgtcgatct gatttatggt gatgtgattg ccgatgccca
     2881 accgctggat ttgctggtga atagcagtta tttcgcgatt aactgagaga gcgtttgccc
     2941 attgcctgat gcgatgctaa cgcgtcttat caggcctaca aatcgctcat tccgcaggcg
     3001 ggataaggcg ctcgcaccgc ttccggcgac caacgtcatg cttcgtctgg ttgccagcct
     3061 tccgtatacc agatatgcaa cagcgcataa gaacgccagg gcttccagcg ctcggcataa
     3121 cggcggattt gtgccggtgt catccccaga aatcgctgtt taatcagata atcatccggc
     3181 agaaaaacat ctttcgcctg ccagccgcgc aaggcgaaat aattcgccgt ccagcgcccg
     3241 atacccggaa aagtttgcag cgttttcatc gcctgctcca catcgcccgg tattgtcatt
     3301 ggtaaggtgc cctccagcgc cgcatttgcc agatgaatca gcgcctctgc ccgtttcaac
     3361 ggcataccta acgcttttaa tgcctgcggg tcggctgctg ccagccgctg aggcgtgggg
     3421 aagcagatat actccggaaa atcatccagc cgttcgccat aaagctgtgc cactctggcg
     3481 gtcaattttg ccgccatcgc cacgctcacc agttggccta aaatcgcccg cacgccctgc
     3541 tcaaaagcat caacactgcc gggtaaacgc aatccgggcc gcgccgcgcc taacttgccc
     3601 aacgcaccgt taacaatctg cgggttacat tgcagatcaa gcaggcggct cattttcgcc
     3661 agacactcag cggcaacggg ttctaaacct gcacttaaat ttatgtgcag agtatggcgg
     3721 gctatatccg gaatagcagt caccacgccg cgatattcgc ccactgccag actacgggca
     3781 taataactgt ccgcgaccgt ttccacacca ctcaccgcac gggcggcgag aaatcccaac
     3841 atccacgacc agtcatacgg cggctgccag ttcaggatat acatcgcatc tccttattca
     3901 tccgctttca gcataaacgt tattcagacg ttacgctttg ctttcatatt ccggttgtcg
     3961 cgacggcaac atttcgctaa agtcacgccc cttcttcacc ggcatgggga ttatttcgtg
     4021 tttattggtt ttgattacgg tacagcaaac tgttcagtgg cggtcatgcg tgacggtaaa
     4081 ccgcatttgc taaaaatgga aaacgacagc acgctgctgc cttcaatgct ttgcgcgcca
     4141 acgcgtgaag cggtaagcga atggctgtac cgccatcatg acgttccggc agacgacgat
     4201 gaaacgcagg cgttgttacg acgggcgatt cgttataacc gcgaagaaga tatcgatgtt
     4261 acagcgaaaa gcgtgcagtt cggtctttcc tcactggcac agtacattga tgatccagaa
     4321 gaagtgtggt ttgtgaaatc accaaaatcg ttcctcggtg ccagcggctt aaaaccgcag
     4381 caggtagcgc tgtttgagga tctggtctgc gcaatgatgt tgcacattcg ccagcaggcg
     4441 caggcgcagc tgccagaagc aattactcag gcggtgattg gtcgcccgat caacttccag
     4501 gggctgggcg gcgatgaagc aaacgcccag gcgcagggga ttctggaacg cgcggcgaag
     4561 cgtgccgggt ttaaggatgt ggtattccag tacgagccgg tagcggctgg gctggattac
     4621 gaagccacct tgcaggaaga aaaacgggtg ctggtggtgg atatcggcgg tggtacgact
     4681 gactgttcat tgctgctgat ggggccgcag tggcgttcgc gtctcgatcg tgaagccagt
     4741 ctgctgggtc acagtggttg tcgtattggc ggtaacgatc tggatatcgc cctagcgttt
     4801 aaaaacctga tgccactgct gggtatgggt ggcgaaaccg aaaaaggcat cgccctgccg
     4861 atcctgccgt ggtggaatgc ggttgccatc aacgacgttc ctgcacagag tgatttctac
     4921 agtagtgcca acggtcgtct gcttaacgat ctggtacgcg atgcccgcga accggaaaaa
     4981 gtggccctgt tacagaaagt ctggcgtcag cgtttaagct atcgcctggt gcgtagcgca
     5041 gaagagtgca aaattgctct ttcaagcgta gcggaaaccc gcgcctcact gccgtttatc
     5101 agcaatgaac tggctacgct gattagccag cgagggctgg aaagcgccct cagccagcca
     5161 ctggcgcgga ttctggaaca ggtgcaactg gccctggata acgcccagga aaaaccggac
     5221 gttatctacc tgaccggcgg tagcgcccgt tctccgctga ttaaaaaagc gctggcagaa
     5281 cagttgccgg gcattccgat tgcaggcggc gatgactttg gctccgtcac cgccgggctg
     5341 gcacgctggg cggaagtggt atttcgttaa aagaaaatgg ctcagcagcg gcttaaatca
     5401 gcctgcgcct gagccagttt ttctgccgcc tgacgtaacg gttccatcaa cggcatggtc
     5461 cgtgctggag catgaaggcg gaatcgctgc aactctgtcg cgccgacagt caacgtcgat
     5521 tccagccgat gtcgtttggc ggccattttc gccattaccg cctgtctgtc tgctggcgtt
     5581 accgcttcgt tcggcctgaa cacaaaacgg cgttcacagc tcgctttcca gtcgatgacc
     5641 gcctgggtca gatgatcacc aaacccttta acttgcttaa cgccacgacg ggtaacacct
     5701 gctgctgttt caataccaaa agaccataac gccgctttac gcgcagggcc aacgccggga
     5761 atagaggcaa catcaataaa aaatccctcc agaaacttct acttctgccg ttcccttgca
     5821 gtgtcatgaa gtgctgccag agcgagtttc tcttcttccg gtaacccgag aatttcgtcc
     5881 ttcatttttt cgagcatcgt ccgtttggcg ataaaacctt ccaggccgcc tacctgctgg
     5941 atctggcgga ctaaatggtc atagtccatt ttcgcacgat taaatgcctc acggcgttgc
     6001 tgaacttctg ctttgtacgc tttgcttgtc agactgccaa taatccagat agccgccagc
     6061 acaggaacca aaataatata acgcggttct gcctgaaggc cgcaaagcag cagcgataat
     6121 gctgacagtg cgatctcaag cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc
     6181 ggcctgccag tcggttggaa atgatcgggt aatggcagtt gcaatgctgg cggtgctact
     6241 gacgccatca ccatcgccca gacttttgcc agcacaaaat ccccgccggt ggtaatgacc
     6301 tcttcgccga gatcaataaa atagataacg ccttgattat ccagcgtaca ccacaggcag
     6361 tcggtcaaat gagcgggata aacatgcatt gccgaaacgg gacatttctt taactgttgg
     6421 cgcagagaat caagcgccgc tacccacgct ttagccgtcg gacgcccggt tgccacgcca
     6481 ctttccgtga atgcctgctg aaacatggct tcaacatcac tcggtaacat cgatagcgga
     6541 atcgatcgtg gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg
     6601 aaatgggtaa tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa
     6661 taaggatgcc ggccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattaccg
     6721 tgattttctg tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc tggcggcgta
     6781 aaatgcgaca cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag
     6841 tcgctatcaa tcaacaccac tttgctgtcg cgacctacca taaaactgtt ctggtttacg
     6901 tcacccacga catgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca
     6961 acatagagta gaaaatccca cgcacaatga gggtaacgtt gacgacgatg tgccgggcta
     7021 tagatcatat gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt
     7081 ccgcctcgtc caccgtgaag cgttgcctgc ggccaggcga cataattcaa caattgcgcg
     7141 tcagctgtcg cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga
     7201 taaatcttgg cgacgctatc gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct
     7261 ttgcccagtt cacggcctgg cgtaacgcac tcgccagtag cagtatataa agtgggtttc
     7321 atggtaacca cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg
     7381 ggctggacaa aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg
     7441 acgccagtcc attgaaaaac ggggtaaaga aaggcacatg cggagaatta tccaacatat
     7501 ttaacgccag tcgctggata ccatccgtaa atgctgcaac tttatgcaca cgctcagtgc
     7561 tggtgaaagt ttccagtctg gaaacggcat cttcatcggt aatgaagtgc gtcatattgg
     7621 catattcacc caccatcggc gtaagcggca attgcagacc gtgaccaaaa tccaccacca
     7681 cgccgccatc gccaatctgc ataatcaagg tgccattagc cgatgaaatc aatcccagaa
     7741 acgtgcaggc aaaatcgcga actgctaatt ctttggcttc cgcctcggca aaaagtcgct
     7801 gacgaacggt cagcacaata tccgtggcga ggatatcatt aagccccaat tccccaccct
     7861 gcactttttg cgacatataa gccatcgctt cattgacggc gagcatcgcg ccttcgcccc
     7921 cctgcgaaac gctacctgcg ccatcagcaa ggaacatcac cagtaatggc tgctggtcat
     7981 ttagccaggc aacctgcatc tggcaggcat cctggcaagg caagtccgcg ctgatgtgcg
     8041 acgttccaac agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg
     8101 cgcttccagc acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga
     8161 taaccagcta aacagttcac ggaattgtaa tccttgcaga ggcaaaggct gacgaacgct
     8221 gatttgtgcc agagtcttca tatccgcccc ctgaacaccg atggaaaaga aagcaaacct
     8281 tttatcttct tccccccgaa acactttgtt ggctgcagcc tgccactcat cggttggtgc
     8341 accatcggta atcaggaaaa tccacgggcg ataatacgaa ataccatttg cacgatactc
     8401 acgtttccgc tcctctacca tatccagggc tttggtaatg gccgcaccca ttggtgtatc
     8461 gccctgggca aacaggatgg gcggaaaaaa attagcggcg ctggtaaagg gttgttccac
     8521 atgcaccggg ccgaacgtca caatgccgag ttccactctt tttaaagcca gcgaatcggc
     8581 aagcagctca tcgcgaaagg taaccaatcc ggtgttaagt tcattaatag gtctgccact
     8641 catcgagcca gaaacatcca gcagcagaat gcaggggcaa cgtggttccg ggttactggc
     8701 aaaatcgctg gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta
     8761 ttgaggtccg gtatcatacc gactgcaata atgaaataag agcaaacgaa gaaactttca
     8821 gaattagtcc cggcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc
     8881 gattgacact aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc
     8941 ccgttacttt attcgtaccc cttataatgg ggtgttagcc agccaga
//
LOCUS       DAJTYF010000139         8911 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTYF010000139 DAJTYF010000000
VERSION     DAJTYF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.139"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..303
                     /locus_tag="OO590_003542"
                     /pseudo
     CDS             1..303
                     /locus_tag="OO590_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(333..3245)
                     /locus_tag="OO590_003543"
     CDS             complement(333..3245)
                     /locus_tag="OO590_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638768.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3248..3364
                     /locus_tag="OO590_003544"
     CDS             3248..3364
                     /locus_tag="OO590_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6638769.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3728..4570
                     /locus_tag="OO590_003545"
     CDS             3728..4570
                     /locus_tag="OO590_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6638770.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4573..5661
                     /gene="capU"
                     /locus_tag="OO590_003546"
     CDS             4573..5661
                     /gene="capU"
                     /locus_tag="OO590_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6638771.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNEPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5666..6616
                     /gene="virK"
                     /locus_tag="OO590_003547"
     CDS             5666..6616
                     /gene="virK"
                     /locus_tag="OO590_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6638772.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6681..7625
                     /gene="lpxM"
                     /locus_tag="OO590_003548"
                     /gene_synonym="msbB"
     CDS             6681..7625
                     /gene="lpxM"
                     /locus_tag="OO590_003548"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6638773.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(7779..8811)
                     /locus_tag="OO590_003549"
                     /pseudo
     CDS             complement(7779..8811)
                     /locus_tag="OO590_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         2325 a         2046 c         2124 g         2416 t
ORIGIN      
        1 taagccctcg tcagcagttc cgtcagcact gcgacagcgt tctttacccg gtcaaaacag
       61 cgttgcggtg caccacgcct gacggatgaa ctgcgtgctc agggttacca ctttaacgta
      121 aaaaccgtgg cggcaagcct gcgccgtcag gggctgaggg caaaggcctc ccggaagttc
      181 agcccggtca gttaccgcgc acatggcctg cccgtgtcag aaaatctgct ggagcaggat
      241 ttttacgcca gtggtccgaa ccagaagtgg gcgggagaca tcacgtactt acgtacagat
      301 gaagttcggt tacatcctgt cagcacggaa cctcacgctt tttgagcggc acgacggatc
      361 ccccccaccc agtatccgac agacttttca ctgcgttcgt aaaaaccctt cttccctccc
      421 agcatatcca ctaattccgg tgacatatcc tgcagataag tctgcaaatc atgccccaga
      481 tcttccggtg actgcaactc ataaaccgcg cgccacacat caccctgcct tattttaagc
      541 cccgggttaa gaatatactg tccgtggttc agcctcagaa aaagcttttt tccataagga
      601 tttttgctgt taacttcatg ttgagagagc tggctgctga tataactcct tcgtttgcgg
      661 cgttccggca gcaatgcttc aggaagatga accacacact ctgccagtcg tgccgcactg
      721 aaggcttccg aaaaaaacag agcacttccc agatgcgtgt ggaacagcgc ctgcatcagg
      781 atcaccagaa acattgccat ctgccggttg tccagtttga tgaggcgtcc ttccacctgc
      841 agtgacagac tttcaggttt cagcagggta tacaactgcg cacttttctg ttgcgcataa
      901 cggggcgtac tgaccgccgc tgacagaact tgatgataag ccgccagccc gtcattaccg
      961 gtcagcaagg gatctgcacc acgctccagc agtaactgaa ccagtgcaat attacctgcc
     1021 cgggctgctg acatcagcgg agtaagatta aacggcgaac ggtgatcaac accataacgt
     1081 tcaatatccc gcaacacggc aaccgggtta ttcattgagt atggccggaa atacttcagc
     1141 tccagtaact gcagagtctt cgttctgggc tgccttgtgg gcttaaaatc ttcccggtac
     1201 agtgcattat acagcgggaa cagggaataa atcagactgt actccagcgc ctgcagttgt
     1261 atggttttat cggctgtacc tgcgaggata tgctgtttcc actgtcgtgc atcctctgcg
     1321 gtgataatct gccatggcat ctcagatgtc tgcaggatcc ggctacggat ggtgtccgcc
     1381 tgctcctgct tcccctgttt ttcaagcaga tgtgcggttt tctgccactc atcacggctg
     1441 gaaatctctt cctgcagatt cagaggctct tcctgatgcg tgagcgcaag cagactccac
     1501 agaggatgct ccacctgctg ttcaacaaga tacacattgt gtgtagcacg ggtgagcgca
     1561 acatacagcg cgttggtaaa aaatttatag atttccgctg aacgatcctg cctgtcccgc
     1621 ggacgggaat aacgggcctc gccctcaaga tcagccggtg taagaccctc gcaaatatca
     1681 tcaaatgcct gccgggcagc gcttactata ttgtaaagga tcaccgtttc gtattccagc
     1741 cccttaacct cctgaaccga aaagaccagc ggcgtactga accagcatcg cgcctgtgct
     1801 ttctgttccg gatgcatcac aatgaccgct acacgattcg aaagtgaggt tttggcattc
     1861 agttcctgct ttgtctcttc cctgtcgtcc agcaaccgga tgctcccttc tgcctgaccg
     1921 cagcttctga caaaatggtg actttcccga tcaatagcac taaagcggac ctgtttgagt
     1981 ctcagcaacc ggttagccag tgcagtgacg tggtgcccgt tacggtaatt cgcctgcagg
     2041 atattaacgg ttgtgtcgtt tccctgttgc tgccggaaaa agagactttt gagcgaactc
     2101 catgagaaaa agctcggatg aactatctga ttggcatcac cacagagtaa aaactgaccg
     2161 ggatgacgta gcgttttcag taccagttgc agttgaacag gcgtcatatc ctgaacttca
     2221 tcaacaaaaa tgacatcata acggggagtt gccaacgaaa gatactgatg actgagcaga
     2281 ttactgtcat actgatgcga ctgtttcagc catgcgatat aacgttcaaa caggacatag
     2341 accgtcgggc ggtcctccat gccatagagc gactgccgta tccccagtga cagatacgcc
     2401 tcccgggaca gcggcccatt cccggaggca accgcaccga tcacgcccct gaactcctca
     2461 tacagggtat gggcagcccc cagtgcggcg atagccctgt tgcggggcag ccagtcgctg
     2521 aacgcactga aagtcacctc cctgccttcc ggaatgcgca gagtttccag aaattccgtc
     2581 agcgaaagaa agtcaatatt ctgtacctca ctcccttccc ctgaagcgtc atagagagta
     2641 cgcgcttttt ccaccagaaa tgaggaaagg ctgagataca gtatatcacc agcagcctgc
     2701 ttcatttttt ccagtaccag cgccgtctta ccacttcccg ccgagccaac aatcaccagc
     2761 ggtagcgggt gttcgtacag tgcctgctga tcatcatcaa aggacagcat tttatccagg
     2821 cggtggaaac gcccatgaga cggattgata tacgtcagcg cttcagtcgc gatatcaaca
     2881 ggatccggca ctggttgttg ttgtaagcgc ccttcatcaa tcaccacgtt tctgcgaaga
     2941 aaacgtgaag tgttataggc atgattgcga atatattcca gaacaaggca gatagtttca
     3001 ccgtggtagc gatacagcga aaacagtaat cggtcccgga tactgagccg ggcgcggtaa
     3061 agattggtgt caattttacg aacatcagcc tgaacaaagt tatcggtggc aatagcctgt
     3121 gcaaattttt tataacccgg gatcgtgtca ggattcagag tatgataaag acagattttc
     3181 atggctgccg gtttccgtcg tgcaaaaaac atgctgattt tacgaaggaa tgccttactg
     3241 aacaaccttg cccggaatac cccccgaaat cagatggaac aatcagaaaa atgctctgca
     3301 gttatggaat aaagaaggtg gggtggtacg ccgggagggc gatccgctgg aaatggcgca
     3361 gtagctgcag gcctgtcatg atgccggaat agaagttcgt gtacaggtta atgaaagcgt
     3421 caccccgtaa ataaactctg cgctgaaatt cctttttttc attggagcac ctgtgttgtt
     3481 ctgaggtgaa catatcacct ctgttcaggt agccaaattc agtgtgccac ctcaaggttc
     3541 ttagtgagtc gtttttaaaa gatatgataa tatatcattg tttatgaatt gttgatgttt
     3601 aatttagtaa attatatcaa atatgccatg ggtttgctat gttattcatc cagtatctgg
     3661 aaataaaatg caccagcttt attgggtttt ccttttgtct ctgcttacta ggtgccagaa
     3721 actttaaatg ttaaacgaag ggggcatttt gttcaaagcc aatcatgttc cggtattaat
     3781 gtatcatcat gtcagtcatt gccccggact ggttacactt tctcccgtga cattccggaa
     3841 gcaaataaaa tggttggctg aaaacaactg gaaaacactg tcatcagatg aactggagtt
     3901 tttttatcgg ggcggaaaac tcccccggaa aagcgttatg ttgacatttg atgatggata
     3961 ccttgacaac tggtttcagg tatatccttt attaaaagag tttaatctca aagcacatat
     4021 ttttttaatt accggtttta ttgggaatgg tccggttcgt cactctcctg gaaaggaata
     4081 ttctcatcgc gattgcgaac atcaaatagc gacagggaat gctgataatg ttatgcttcg
     4141 ctggtctgaa gtaaacgaaa tgttgcaatc tggtctggtc gaatttcatg ttcatacgca
     4201 tactcataca cgctgggata aaaagttcag ttcacgagaa gaacagtgta agcatcttcg
     4261 gcaggacttg ttgagcggga gagaatattt gaaagaaatg acaggaaaat gcagtaaaca
     4321 tttgtgttgg ccggaaggtt attataacaa ggattatatt caggtcgctg aagaacttgg
     4381 attctattat ttatatacaa cagaaagaag aatgaatgct cctgctaaag gaactacacg
     4441 gataggaaga ataagcacca aagaacggga aagttgcgca tggctgaaaa gacgactttt
     4501 ttattataca acaccgtttt tttcatcgtt gctggccttt cataaggggc caagattacc
     4561 ggatgattaa taatgaatat actatttacg gaatcatcac cgaatatagg tggtcaggaa
     4621 ttacaggctg ttgctcaaat gaaggccctg aagaaaatgg ggcattcagt tctgcttgtc
     4681 tgcagggaaa acagcaaaat tgcttttgaa gccagtaaat tgggaattga tatcacattc
     4741 gcgttatttc gaaacagtct tcacatccct actgcatgga gattactcgg aatagttcat
     4801 ggttttcagc ccaatgcaat cgtttgtcac agtgggcatg atagcaatat tgttggttta
     4861 gtacggttat tcactaggaa acatccattc agaattatca ggcaaaagac atatttgaca
     4921 cgaaaaacaa aagttttttc aataaatcat ttttgcgatg aggtgattgt tcccggaaca
     4981 agtatgaaga cacatctgga gcaggaagga tgtcgaaccc gggttactgt tgtgcctcca
     5041 ggctttgatt tccagaaatt atacgttgat tcccgaaaca gtttgcctcc aaatgttctt
     5101 tcttggctgg cgtcccgaag gggatgcccc gttattgctc aggtaggaat gttgcgcccg
     5161 gaaaaagggc acgaatttat gttgaattta ctgttccatt taaaaatgaa tggacgacag
     5221 ttctgttggt tgattgtggg gtctggttcg cctgaactgc gggagcattt acagtatcag
     5281 attgacagta tgggcatgca tgatgatgtt tttattgctg acaatgtttt tcctgccgcc
     5341 cccgtatatc gggttgccag tctggtggtt ctgccttcag aaaacgaatc gtttggtatg
     5401 gtactggcag aagcatcggc attttctgtg cctgtactgg ccagtcagat tggtggaatc
     5461 cctgatgtta ttcagaacaa ccagaccggg acattgttac cagcaggtaa taagcacgca
     5521 tggatgtgcg ccctgaatga tttttttaat gaacctgggc gtttttatca gatggctcgc
     5581 caggcaaaac aggatataga agagcggttt gatattaata aaactgcgtt aaaaatactc
     5641 acattagcga agcacaagta acaatatgtt ttctgtaagt aacttatcat ttatcggttt
     5701 ccttaaaagg attgtttttt cctcagattc actcccgggg aagtgggaac acagaaaatt
     5761 ccggttcatg tacattttgc gatgtgctat aaatccggtt gccagtattc gatattatta
     5821 cgaactgcgt tccttgcagt gcattgagga tattctggca atacaaccca cgttgccagc
     5881 aagaattcat cgaccttatt tgcataaagg ggggcgagcc tggtcccggg ggcaatatat
     5941 tcttgagcat tatcgctttg tgcaaaattt gccagaaaaa tattctgaat ttttgtttcc
     6001 acaaaaatcg gtatcattag tccaattcat cgggaaagat ggagaggatt ttgatatcca
     6061 gtgttctccc agtggttttg accgggaagg agagttgatg ttgtcattgt ttttcaatca
     6121 aatagtgatt gcccggttaa cattttcagt cattctgact caaaatggac acaccgcttt
     6181 tattggcgga ttacagggag cccccaaaaa taccggacct gatgttattc ggtgcgcaac
     6241 ccgggcctgt tatgggcttt tcccaaaacg gattattttt gaagcatttt gtgcgttgat
     6301 gaaagcttgt aatgtctctg agtgtctggc agtcagtgag cacagtcatg ttttccggca
     6361 attgagatac tggtatcaga aacgcaaaac ctttgttgct gtctacagtg atttctggga
     6421 gtctgtagca gggaaaacct gtggtgactg gtacaaatta ccaacacagg tggttcgaaa
     6481 gccgctaagc aatatagcta gtaaaaaacg ctctgagtat cgaaaacgat acgctttgct
     6541 ggattatatc catgaaactg ctatccgctc tttggatgca tatcctgtaa actcagaaca
     6601 tcaggactta aattaaggtt tgatgtattc tctgaataaa atattaatga tgattttggt
     6661 aggggcattc gcactaaata atgaaaaaat acaaatccga gtttattcct gaatttaaga
     6721 aaaattatct ttcccctgtt tactggttta catggttcgt tttgggaatg attgcaggta
     6781 tttcaatgtt tcccccttca ttcagagatc ctgtcttggc caaaataggg cgttgggtgg
     6841 gcagattgag cagaaaagct cgtcgcaggg cgacgattaa tttatcgctt tgtttcccgg
     6901 aaaagagtga tacagaacgg gaaataattg tcgacaatat gtttgccaca gcattgcaat
     6961 ccatagtgat gatggcagaa ctggcaattc gtggtccgga aaagttccag aaacgagtgt
     7021 tctggaaagg cctcgaaatt cttgaggaaa tccggcacaa taacagaaat gtgatttttc
     7081 tggttcccca tggctggagc gtggatattc ctgcaatgtt gctggcagct cagggggaaa
     7141 aaatggccgc catgtttcat cagcaacgaa atccagtgat tgattatgtc tggaattctg
     7201 tacggcgtaa attcgggggg cgcttacatt cccgggagga tgggataaaa ccatttattc
     7261 agtcagtacg ccagggatac tgggggtatt accttccaga tcaggatcat ggtcctgaat
     7321 acagtgaatt tgctgatttt tttgcgacct ataaagcgac attaccaatt attggacgtc
     7381 tgatgaacat cagtcaggct atgattatac cgcttttccc ggtttatgat gaaaaaaaac
     7441 atttcctgac tattgaagtt cggccaccaa tggatgcatg cattgccagc gcggacaata
     7501 aaatgattgc ccgacaaatg aacaaaacag tggaaatttt ggtggggtca catccggaac
     7561 agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc aaacgaagcg gacccgtacc
     7621 cttgaaatta taaagtacag gtatttccac tagttgtttc ttacaggtta ccaatcgaaa
     7681 cacatccccc ttccgtgtgt tgcccgtttg gcacggagga ctgtgtagat gcagttcgtt
     7741 aaatttaatt tatgcctaaa tagctgcgct taataccgct acacttttgc cagcccatgt
     7801 ttgcctccgg ggaatgggct gacggtttcc ataaaatggc gaactttttt caacagttgc
     7861 cacattgagc ggcactgatg attacgcgtt attgtgtcgt gaagtgcctg ccatagccgt
     7921 tcaacatgat tcacccatgg cgagtaaacc ggctgataaa tgcccctgaa cttcgggttc
     7981 tccttcagcc agctctgtgt ttcccggctt ttgtggataa tgtagttgtc cacgatcagc
     8041 gtgatggttt tcgcccgacg gtatgtcgct ttaagccgct tcagcaggct gatgtaacag
     8101 cgccgaactt ttgctgttgc cgcccacata gctgacttta cctgtcccgc tgtgcagcgc
     8161 tccggccaga taatattttt cattctgtcc cggcgtgacc acccgttttt gctgtccgcg
     8221 cagttgccag tccgcaccga ttttgggatg aagatggata tccacttcat cttcataaaa
     8281 gaccggatgc tctgcgctgc attcgtccag tgctttatgg attgctgcca tcttttcatc
     8341 tttatgcggg tcacggatac gcagagttgg cgcagccctt cgccacacaa tccccgcaga
     8401 cggcaaccag cggcgaacgg ttccggcatt taactggcaa ccggttatct cattgatttt
     8461 tattgccagc agttctgtac tccagcgtga acgctggtag ccaaagtcgc cgggagaatg
     8521 ttttaccagc tcacgtaaca gtgtgcagat atgctcaaac ggccagcgac gggcacgccc
     8581 ggcaggtaat gatttcagtc cctcaacacc cgactgcgtg aaccagttaa tccagcgtcc
     8641 aacagaggaa cgggcgcagc agagcgttct ggcaacgtcg ctgacacggt cgccccggtg
     8701 cagcatcagc atggcagtca gtctgcgggc ataattttta tcgtgtgttt tatggatggc
     8761 tttctgcatc aggcgtcgtt cgtcacggga aattggtgct atgatcggca ttgctcagtc
     8821 cggttggtga tttgttttga tttggcgatt gatcagatcg cacaatccgg gctaagttcc
     8881 ctcaaaggga tctactattc cgcgcagcta t
//
LOCUS       DAJTYF010000140         8904 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTYF010000140 DAJTYF010000000
VERSION     DAJTYF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.140"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO590_003550"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO590_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6638774.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO590_003551"
     CDS             1828..3147
                     /locus_tag="OO590_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6638775.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3433..3474
                     /locus_tag="OO590_003552"
     CDS             3433..3474
                     /locus_tag="OO590_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638776.1"
                     /translation="MVQLVDLARCVSF"
     gene            3512..4657
                     /gene="dosC"
                     /locus_tag="OO590_003553"
     CDS             3512..4657
                     /gene="dosC"
                     /locus_tag="OO590_003553"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6638777.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4582..5199
                     /locus_tag="OO590_003554"
                     /pseudo
     CDS             4582..5199
                     /locus_tag="OO590_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5457..5789
                     /gene="ddpX"
                     /locus_tag="OO590_003555"
                     /pseudo
     CDS             5457..5789
                     /gene="ddpX"
                     /locus_tag="OO590_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5803..6699
                     /locus_tag="OO590_003556"
     CDS             5803..6699
                     /locus_tag="OO590_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6638778.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6696..7682
                     /locus_tag="OO590_003557"
     CDS             6696..7682
                     /locus_tag="OO590_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6638779.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO590_003558"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO590_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..>8904)
                     /gene="osmC"
                     /locus_tag="OO590_003559"
     CDS             complement(8657..>8904)
                     /gene="osmC"
                     /locus_tag="OO590_003559"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6638780.1"
                     /translation="ALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKSEVAV
                     PGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2167 a         2178 c         2250 g         2309 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acacttagtt aaacagctat aaatccctaa taaaaatgta tgaatatgaa
     3301 aaaccatacg atattcgctg tcaccgaaaa ataattacga tgggctatac tcttctgaag
     3361 aacagtagcg tagattgaaa ctgtcttaag tcgcttattt cttttcagta tatcttcata
     3421 tttcaggaga atatggtgca gttagtcgat ctcgcacgtt gcgtttcgtt ttgattccgt
     3481 ctgatttccc agccttataa gggtgtgaat tatggagatg tattttaaaa gaatgaaaga
     3541 tgagtggacc ggacttgtcg aacaggcaga tcctctcatt cgtgctaaag ccgcagaaat
     3601 tgcccttgcg catgctcatt atctgagtat cgagttttat cgaattgtcc gcatcgaccc
     3661 gcatgccgaa gaattcttga gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga
     3721 acgctggatt attaacgtgc tttctgccca ggttgacgat gtcgaaaggc taatacaaat
     3781 ccagcatacc gtcgcggaag tgcatgcccg cataggaatt ccggtagaaa ttgtcgagat
     3841 ggggtttcgg gtgctgaaaa aaatcctcta tccggtaatc ttctcttcgg attattccgc
     3901 cgcagaaaaa cttcaggtct accatttctc gattaacagt attgatatcg cgatggaagt
     3961 gatgacccgc gcgtttacct ttagtgacag tagtgcctca aaggaagatg aaaactatcg
     4021 tatcttctcg ttactggaaa acgccgaaga agaaaaagaa cggcaaatag cctcaatact
     4081 ttcatgggaa atagatatta tctataaagt cctgctggat tctgatttag gcagcagttt
     4141 gcctttaagc caggctgatt ttggcctgtg gtttaaccat aaaggtcgac attattttag
     4201 cggcattgcc gaagtgggcc atatctcccg tctgattcag gatttcgacg gtattttcaa
     4261 tcaaaccatg cgtaacacca ggattttgaa taacagaagt ttgcgggtga aatttctatt
     4321 acagataaga aataccgtat cgcagattat taccttgctg cgtgaattgt ttgaagaagt
     4381 atcgcgccac gaagtcggta tggatgtact gacgaaatta cttaaccgcc gtttcctgcc
     4441 gactatcttc aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct
     4501 gattattgac gttgataaat tcaaagagat caacgatacg tggggccata acactggcga
     4561 tgaaattctg cgtaaagtct cattcctctc gcagaagaga ttggtgaaat cgaaaatatt
     4621 gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt
     4681 catatcccgg cgttatccgt gaacttgtcg gcgctgcact ttcgcagtaa tcagctgcct
     4741 aatcaggtgt ctgatgcaat gcaagcctgg ggtattgacg gccaccagct gacggtggaa
     4801 atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg
     4861 cgtgatatgg gcgtgggttt atcggtagat gattttggca cgggcttttc cggattatcc
     4921 cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagctttgt cgatcgttgt
     4981 ctgacggaaa aacgcatcct ggccttactt gaagccatta ccagcattgg gcaaagcctc
     5041 aatttaaccg tcgtggcgga aggggtcgaa accaaagagc aatttgagat gctacgtaag
     5101 atccactgtc gcgttattca gggatatttc ttttcccgcc cccttcccgc cgaagaaatt
     5161 ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct cgcccgcact
     5221 cgcgggtttt cctttaaggt gacactgtca cttaaatact gtgatttcag ctacgattcc
     5281 gggagattcc ttccttaact ttccctcttg caccaaaact gcactacgct gcacaaatgt
     5341 tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc ccggcatggc
     5401 atgagatctg cataagcgga aagcgcagca atttttgtct tatacaggaa ccgtttatgt
     5461 cggataccac cgaactggtt gatttagccg tgatcttccc tgatctggag atcgaattga
     5521 aatatgcctg cgttgataac atcacaggta aagctattta tcagcaagcg cgttgtctgc
     5581 tacataaaga tgcgattacc gcgctggcga aaagtatcag tatcgcccag ctgtcaggct
     5641 tacaactggt gatttacgat gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag
     5701 cctgcccaga cccgcaatat gttgttgatg tgacggtcgg ttctaatcac agccgtggca
     5761 cggcgctcga cctgacgctt cgtgatgacg tggaggtggt gaatgatgct aagcgaggaa
     5821 acgtccgccg tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg
     5881 ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gcgactgatg
     5941 atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt aaccgcccgc
     6001 cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttt
     6061 agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtggt ggtcgccatt
     6121 gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac
     6181 gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg
     6241 gcactggctg ccgctctcgg gccgagttta tttaacgcca tgctggcgat tgctattgtg
     6301 cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc
     6361 tatgttcagg cggcgaaaac ctttggcgcg tctcgttggc atctgatcaa ctggcatatt
     6421 ttacgtaact ccctaccgcc gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt
     6481 ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag tgctgaatgg
     6541 ggcgcgatgg tagcgaatgg tcgcaactat gtactcgatc agtggtggta ttgcgcattt
     6601 ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga tggtattcgc
     6661 gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt ctggacattc
     6721 aacagctgca tttgagtttc cccggtttta acggtgatgt tcacgcgctc aacaatgtgt
     6781 ccttgcagat taaccgcggc gaaattgtcg gtctggtggg agaatccggc tcaggtaaat
     6841 cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc gtacatcggg
     6901 ggcagatttc actgctggga gaagatgttc ttaacgctcg ggaaaaacag cttcgtcagt
     6961 ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctt aatccgacac
     7021 gtcgaatagg tcttcagatg atggacgtaa tccgccatca tcaaccaata agtcgtcggg
     7081 aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat gccgtggaag
     7141 ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta atgattgcgc
     7201 tggcattctc ctgcgagccg caattgatta ttgccgacga accgacgacg gcgctggacg
     7261 tcacagtaca gttgcaggta ctgcgtttgc ttaaacataa agcacgcgcc agtggaactg
     7321 cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat agcgtttacg
     7381 tgatgtatgc cggaagcgta attgaaagcg gcgggacggc agacgttatc catcatcccc
     7441 ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta ccacgccagc
     7501 cattacccgc cattccaggg acggtaccaa acctcaccca tttgcctgac ggctgcgctt
     7561 ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggtaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttactgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gagctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgagctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gcca
//
LOCUS       DAJTYF010000141         8711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTYF010000141 DAJTYF010000000
VERSION     DAJTYF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.141"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(13..753)
                     /gene="cysQ"
                     /locus_tag="OO590_003560"
     CDS             complement(13..753)
                     /gene="cysQ"
                     /locus_tag="OO590_003560"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6638781.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            943..2886
                     /gene="cpdB"
                     /locus_tag="OO590_003561"
     CDS             943..2886
                     /gene="cpdB"
                     /locus_tag="OO590_003561"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6638782.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3012..3392)
                     /locus_tag="OO590_003562"
     CDS             complement(3012..3392)
                     /locus_tag="OO590_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6638783.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3481..4341
                     /gene="qorB"
                     /locus_tag="OO590_003563"
     CDS             3481..4341
                     /gene="qorB"
                     /locus_tag="OO590_003563"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6638784.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            4450..5415
                     /locus_tag="OO590_003564"
     CDS             4450..5415
                     /locus_tag="OO590_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6638785.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5523..6185
                     /gene="ytfE"
                     /locus_tag="OO590_003565"
     CDS             5523..6185
                     /gene="ytfE"
                     /locus_tag="OO590_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6638786.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6230..7642)
                     /gene="cycA"
                     /locus_tag="OO590_003566"
                     /gene_synonym="dagA"
     CDS             complement(6230..7642)
                     /gene="cycA"
                     /locus_tag="OO590_003566"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6638787.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7950..8570)
                     /gene="fklB"
                     /locus_tag="OO590_003567"
     CDS             complement(7950..8570)
                     /gene="fklB"
                     /locus_tag="OO590_003567"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6638788.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALIAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         2211 a         2295 c         2207 g         1998 t
ORIGIN      
        1 tgccatctga atttagtaaa tggacactct gaaccccgga ttcaggaacg actcgcgcgg
       61 agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac
      121 agcatgtcca gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc
      181 ctgtccttcc gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc
      241 gccaagctgt tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac
      301 cagcggcggg cgcgcatcgc ggacctgaat cagcttgcgc acaccgcact cttctttcca
      361 ggccttgcct tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa
      421 aatcggtttg ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat
      481 aaactcttta gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac
      541 ttcccaaccg ggaggatctt cttcagaaag gaccggaata tccggtgcca gcgtacgtaa
      601 accgtccatg ataacggtgt gagcggcaat atccgctgcc gttaccggag aattgtccgc
      661 tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc
      721 attccgtgca agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg
      781 ttaacttatt gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca
      841 tcattttcaa agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc
      901 tcccttttct ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca
      961 acgctcctgg ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc
     1021 atggaaacca ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc
     1081 acggaaaaat tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg
     1141 aaaaacagcg tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac
     1201 atgtcggcga aaggattaaa agcaggtgat gttcatccgg tttataaggc gctgaatacg
     1261 ctggattata cggtcggtac actcggcaat catgaattta actacggtct ggattacctg
     1321 aaaaatgcgc tggcgggagc gaaattccct tatgtaaatg ccaacgtcat tgacgccaga
     1381 accaaacagc caatgtttac accgtattta attaaagata ccgaagtggt cgataaagac
     1441 ggaaaaaaac agacgctgaa gattggctat attggcgtcg tgccgccgca aatcatgggc
     1501 tgggataaag ctaatttatc cggaaaagtg acggtgaatg atattaccga aaccgtgcgc
     1561 aaatacgtgc ctgaaatgcg cgagaaaggt gccgatgtca ttgtcgttct ggcgcattcc
     1621 gggctgtctg ccgatccgta taaagtaatg gcggaaaact cagtttatta cctcagtgaa
     1681 attccgggcg ttaacgccat tatgtttggc catgctcacg ccgttttccc gggtaaagat
     1741 tttgctgata tcgaaggggc tgatatcacc aaaggcacgc tgaatggtgt tccggcggta
     1801 atgccgggca tgtggggcga tcatcttggg gtggtcgact tacaactcag taatgacagc
     1861 ggtaaatggc aggtgacgca ggcgaaagcg gaagcacggc cgatttacga catcgccaat
     1921 aaaaaatccc tcgcggcgga agacagcaag ctggtagaaa cactcaaagc cgatcacgat
     1981 gccacacgcc agttcgtcag caagccaatc ggtaaatctg ccgacaatat gtatagctat
     2041 ctggcgctgg tgcaggacga tccgaccgtg caagtcgtga acaacgcgca aaaagcgtat
     2101 gtcgaacatt acattcaggg cgatccggat ctggcaaaac tgccggtgct ttcagctgcc
     2161 gcaccgttta aagttggtgg tcgcaaaaat gacccggcaa gctatgtgga ggtggaaaaa
     2221 ggccagttga ccttccgtaa tgccgccgat ctttatctct accccaatac gctgattgta
     2281 gtgaaagcca gcggtaaaga ggtgaaagag tggctggagt gctccgccgg acagtttaac
     2341 cagattgctc ctaacagcac gaaaccacag tcactcatca actgggatgg tttccgcact
     2401 tataactttg atgttattga tggtgtgaat tatcagattg atgttaccca acccgcccgc
     2461 tatgacggcg agtgccagat gattaatgcc aatgcagagc ggattaagaa cctgaccttt
     2521 aatggcaaac cgattgatcc gaacgctatg ttcctggttg ccaccaataa ctatcgcgct
     2581 tacggcggca aatttgccgg tacgggcgac agccatatcg cttttgcttc accggatgag
     2641 aaccgctcgg tgctggcagc gtggattgct gatgagtcga aacgtgcggg ggaaattcat
     2701 ccggcggcag ataacaactg gcgtttagca ccgatagctg ccgataagaa actggatatc
     2761 cgtttcgaaa cttccccgtc agataaagcc gcagcgttta ttaaagagaa agggcagtat
     2821 ccgatgaata aagtcgcgac cgatgatatc gggtttgcga tttatcaggt ggatttgagt
     2881 aagtaaaaca cttctttttc ggcctataaa tcaaccgcat ccggcattta ttggcggatg
     2941 cgatgctgcc gtatcttatc caccctacaa gccatgaacc gtagaccgga taagcgcagc
     3001 gcatccggca gttatgccgc acgttcatcc cgcaccgcca acacctcggg caaattcaac
     3061 tcaatccagt ccgccagtgc agcaaccttt tcgctcacct gctcgcccag cggcgtgagg
     3121 ctatattcca catgcggcgg caccaccgga tacgcgatac ggttaagaaa accatcctgt
     3181 tctaacgcct gtaacgactg cgcaagcatc ttttcactca ccccaccgat tttgcgccgc
     3241 aggtcgctaa agcgatgagt accttcgcgt agcgccacca gaatcaacac cccccaacgg
     3301 ctggtgacgt gtttcaacac ctcgcgcgac gggcactgtt ccgcaaagag attaccctct
     3361 ttcagttgtt gtgacaggct aacctgactc atttcatact tacctttttg cacgtactta
     3421 ctaaaagtaa gtttaggtgt tagcgtattt aaacacaaga caaaacgatg gagacttccc
     3481 atgatcgcta ttactggtgc cactggccaa cttggtcact atattattga atccttgatg
     3541 aaaacggttc ctgccagcca aatagtggct atcgttcgta atccggcaaa agcccaggcc
     3601 ctggcagcac aaggcattac cgtgcgtcag gctgactaca gcgatgaagc cgcactgaca
     3661 tctgcacttc agggagtgga aaaactactg ctgatctctt ccagcgaagt gggtcaacgt
     3721 gccccgcagc atcgtaatgt tattaatgcc gcaaaggcgg ctggcgtgaa atttatcgct
     3781 tataccagcc tgctacatgc agatacctcc ccgctcggcc tcgccgatga gcacatcgag
     3841 acggagaaaa tgctggctga ttctggcatc gtttacaccc tgctgcgcaa cggctggtac
     3901 agcgaaaact acctcgccag cgccccggca gcactggaac acggcgtatt tatcggtgcg
     3961 gcgggcgatg gcaaaatcgc ctcagcaacg cgggcagatt atgcgacagc tgcggcactc
     4021 gtgattagcg aagccggtca cgaaggcaag gtttacgaac tggcgggcga tagtgcctgg
     4081 acgttgacac agttagcggc agagctgacc aaacagagcg gcaaacaggt tacctatcaa
     4141 aatctgagcg aagccgattt cgccgcggca ctgaaaagcg tcggactgcc cgacggactg
     4201 gcggatatgc tggcggattc tgacgttggc gcatcgaaag gtggtctgtt tgataacagc
     4261 aaaacgctta gcaaattgat tggccgccca acgacaacgt tagccgaaag cgtaagccat
     4321 ctttttaatg ttaataacta gttaattaaa gtggcatcct cccgcatcct ctctgataat
     4381 gacgggatgc cgggagcaat catgtctgct tcctgaacgt ttcttctgac agaccaatgg
     4441 atgccagtaa tgattagcgg cgtgctgtac gccctgttag cagggttgat gtgggggctt
     4501 atttttgtcg ggccgttgat cgtgccggaa tacccggcga tgttacagtc gatggggcgt
     4561 tatctggcgt tagggttaat tgcgctaccc attgcctggc tgggacgcgt gcgtctgcgt
     4621 cagttggcgc gtcgggactg gcttaccgcc ttgatgctca ctatgatggg caacctcatt
     4681 tattacttct gccttgccag tgccattcaa cgtactggcg cgcctgtttc cacgatgatt
     4741 atcggcaccc tgccggtggt gattcctgtc tttgccaatc tgctttatag ccagcgcgac
     4801 ggcaaactcg cgtggggaaa actcgccccg gcactgattt gtattggcat cggcctggcg
     4861 agtgtgaata ttgctgagtt aaaccacgga ctccccgatt ttgactgggc acgttatacc
     4921 tctggcatcg tgctagcgtt agtttccgtg gtctgctggg catggtatgc cctgcgcaac
     4981 gcccgctggc tgcgggaaaa tcccgacaaa catccgatga tgtgggcgac ggcgcaggcg
     5041 ctggtcacgc tgccggtttc tctcatcggc tatctcgtcg cctgttactg gctgaatata
     5101 caaacgccgg acttctcctt accttttggc ccccgtccgc tggtgtttat tagtctgatg
     5161 gttgcgatag ccgtgctttg ctcatgggtt ggcgcactct gctggaacgt cgccagccag
     5221 cgattaccga cagtgattct cgggccgctg attgttttcg aaacgctggc aggtttgctg
     5281 tacacctttt tactccgcca gcaaatgccg ccgctaatga cgctgagcgg tatcgcgctg
     5341 ttagtgattg gcgtggtcat cgcggtcaga gcaaaaccag aaaaaccttt aactgaatct
     5401 gtctcagaaa gttgacatgc tggcagtgag ttaaataagc ctctgctacg taagggttat
     5461 agcttttgcc ttaaaggtgt atttaaaata catcttatct tattaagaat gaggtatcag
     5521 ctatggctta tcgcgaccaa cctttaggtg aactggcgct ctctatccct cgcgcttcgg
     5581 ctctgtttcg taaatatgat atggattact gctgtggcgg taagcagacg ctggcgcgcg
     5641 cggcggcacg taaagaactg gatgttgagg tcattgaagc tgaactggca aagcttgctg
     5701 aacaaccgat tgagaaagac tggcgtagcg ccccgctggc agaaatcatt gaccatatca
     5761 tcgtgcgcta ccacgatcgt caccgcgagc agctgccgga gctgatcctg caagcgacta
     5821 aagtcgagcg cgttcacgcc gacaaaccga gcgtgccaaa agggctgaca aaatacctga
     5881 ccatgctgca tgaagagctt tccagccaca tgatgaaaga agagcagatc ctcttcccga
     5941 tgatcaaaca aggcatgggc agccaggcaa tggggccaat cagcgtaatg gaaagcgagc
     6001 acgatgaagc gggcgaactg ctagaagtga tcaaacacac taccaataac gtcacaccgc
     6061 cgccagaagc ctgcaccacc tggaaagcga tgtataacgg cattaatgaa ctgattgatg
     6121 acctgatgga tcacatcagt ctggaaaaca atgtactgtt cccacgcgcc ctagcgggtg
     6181 agtgatctaa aagctggatg gcattgcgcc atccagcatg ataatgcggt tatttccgca
     6241 gttcagcagc ccgcttctta ccaataaaca gccagcccaa ccccagcgcg ataaaccaca
     6301 gcggggtaac cagcagcgcc tggcgagtgt catcttccag tgtcagcaac acgaccacga
     6361 acacaaagaa cgccatacat acccagcaca tcagcttgcc gagcggcatc ttgtagatcg
     6421 acttttcatg cagatgagga cgctgtttgc ggtacaccag gtacgagcaa aggataatcg
     6481 tccagacgaa cataaacaga atcgcggaaa cggttgtaat catcgtgaac gcgccaatca
     6541 cgctaggatt cacatacaac atcaccacgc cgccgagcag acagatacac gagaaggtca
     6601 accctttcgc gggtactgcg cgcttagaaa gtttagcgaa cgctttcggc gcaacacctt
     6661 cctgcgccag accaaacagc atacggctgg tagagaagac accgctgtta gcggaagacg
     6721 ccgcagaggt cagcaccaca aagttgatca cgctggcagc cgcaggcaaa cctaccaaca
     6781 caaacagctc aacgaacggg cttttctccg ggactaccga actccacggt gtcacggaca
     6841 taatcacaat cagcgcgaag acgtagaaca taatgatacg gatcggaatg gagttaatcg
     6901 cgcgtggcag tgatttctcc ggatctttgg tttccgcagc tgttgtacct accagctcaa
     6961 tccccacgaa agcgaaaacc gctatctgga atccggcaaa gaagccactt aaacctttcg
     7021 ggaaccagcc gccgtcattc cacaaatgcg cgaatgacgc ttccacaccg gtcggtgact
     7081 gaaagtgcat cgccaccatg accaggccga cgacaatcag cgacacgatg gcgacgattt
     7141 tgatcatcgc aaaccagaac tccatctcac cgaacatttt cacggtggca aggttgagcg
     7201 tcagcagcag cactatcacc gccagcgagg cgacccagtc ggagaaatcg gggaaccaga
     7261 actgagcata agccgtgatc gccaccacgt ctgccatacc ggttacaacc cagcagaacc
     7321 agtaagtcca gccggtgaaa tatcccgccc acggccccag taaatcggaa gcgaagtcac
     7381 taaaagattt gtattccaga ttcgaaagca gcaattcccc cattgcccgc atcacgaaaa
     7441 agagcataaa accaatgatc atataaacga aaatgatcga cggcccggca aggctaatcg
     7501 ttttgccgga ccccataaac aacccagtac caatggcacc gccaatggca ataagctgaa
     7561 tatgtcggtt tgtgagattg cgccgtagcg actgttcagc cggagcctga tcatcggcaa
     7621 cgacttttac ctgatctacc atgttttttc ttcctgtacc tgtctgtgtt gttcaggctc
     7681 tacggccttt agtcagtcta tgatacgacg aatctgtatc gggactcatc gatattaggt
     7741 aacaatacgc gggatgaata ctaagattta gatctaatgt taattttatg tttaaagtga
     7801 gtgtcatatc acggtgataa cgcgaaacag ctcacaaaaa tacacgctta cggggtcttt
     7861 gcaacacaaa atagcgccgt ttggcaaact gaagggttta ttgctgaatg cctgctcccc
     7921 tctggttcga ggggaacaga atgcgctgct tagaggattt ccagcagttc gacttcaaac
     7981 accagggtgc tgaacggagg gatggatgca cctgcgccgc gctcgccata tgccagttcc
     8041 tgcgggatag tcagttccca tttagaacct accggcatca gagtcagtgc ttcaatccag
     8101 ccagggatca cgccattaac cgggaattca gcgggttcac cacgagcaac ggagctgtca
     8161 aacacggtgc cgtcgatcag tttaccggtg taatgaacac gaacgcggtc ggtacgtgcc
     8221 ggaattgcgc cttcaccttg gttgatcacg cggaattgca ggccagattc ggtgctattc
     8281 acaccttctt ttttggcgtt ttcttccagg tatttcacac cttcagcagc catcgcctgg
     8341 aaacgctgac gacgaacggc atcggcgcgt tcgtggattt cacgcagcgc gcgatgcacc
     8401 acatcaaccg gcacagccgg atgtttgcct tccagcgcat cggcaatacc tgcaatcagt
     8461 gcttctggca gcagcccttc caggccagat tcactcagtt gttgccctac ctgcaaacca
     8521 atgccgtagc ttgcttgcgc ttcgatggtg tcaaaagttg gggtggtcat cgtatttcct
     8581 ttcttagggt ataaaagtag cgggcagcat atcagccaca cttctttggg taaaactttg
     8641 tcgcaggaat gatgacaaat cccggggaaa aagaaacaat agcaatatcc cgtatgaata
     8701 taataaaggc g
//
LOCUS       DAJTYF010000142         8634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTYF010000142 DAJTYF010000000
VERSION     DAJTYF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.142"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..366)
                     /locus_tag="OO590_003568"
     CDS             complement(<1..366)
                     /locus_tag="OO590_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6638789.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            890..1864
                     /gene="hemB"
                     /locus_tag="OO590_003569"
     CDS             890..1864
                     /gene="hemB"
                     /locus_tag="OO590_003569"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6638790.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1970..2821)
                     /gene="tauD"
                     /locus_tag="OO590_003570"
                     /pseudo
     CDS             complement(1970..2821)
                     /gene="tauD"
                     /locus_tag="OO590_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2806..3645)
                     /gene="tauC"
                     /locus_tag="OO590_003571"
     CDS             complement(2806..3645)
                     /gene="tauC"
                     /locus_tag="OO590_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6638791.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAVTRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQW
                     VNV"
     gene            complement(3642..4409)
                     /gene="tauB"
                     /locus_tag="OO590_003572"
     CDS             complement(3642..4409)
                     /gene="tauB"
                     /locus_tag="OO590_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6638792.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4422..5348)
                     /gene="tauA"
                     /locus_tag="OO590_003573"
     CDS             complement(4422..5348)
                     /gene="tauA"
                     /locus_tag="OO590_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6638793.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDDKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(5615..6130)
                     /locus_tag="OO590_003574"
     CDS             complement(5615..6130)
                     /locus_tag="OO590_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638794.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            6323..6574
                     /locus_tag="OO590_003575"
     CDS             6323..6574
                     /locus_tag="OO590_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6638795.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            6576..>8634
                     /locus_tag="OO590_003576"
     CDS             6576..>8634
                     /locus_tag="OO590_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6638796.1"
                     /translation="MQSYVTVNDLLGVPGMPATAKGIRQALQRFSRALGDVSRRRAGT
                     KAIEYHIDCLPEVTQKALRERYVEQLVATENNVSEVKTVTRTARNPDAVQAVEAYRGS
                     PQLMEERLNALTENQRRVSEARAALAVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAINANAKRGTNRTISRDPLYQWVLKYNRSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQANGRPMSEAYEDFVTEWQRRYADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEIV
                     GILPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRETLRVLNRD
                     LRSAFNALQQDKPLNARQKSAMRELPSWSELIEAIREGVEWYNNRPHSELPMKPNGKH
                     YSPAEFRKKRLAEENTEIEWLSDLELRDMFCPMVERPVRRCEIKWLGNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFPVSADYYKQQQRIKGMHK
                     RAEEKIRDAVDEGIQVIEQKTTEPWLDNVYRPVGNVMTVQQQEYEEEHDEESERNFRR
                     GLQK"
BASE COUNT         2178 a         2229 c         2175 g         2052 t
ORIGIN      
        1 agttgtcatg cactcggaat aaatcatacc gtgaatagtg ctgtttttga ttatcagact
       61 attggcgttg gtattggtag aaataccgtc gtccagatag taggtagaaa tcacgccgtt
      121 aacggtagcg ttattaatga ccgggtagat atcgccgttg taatagctat cagcggcata
      181 gttgttccag cctacataac catcatagta aacgtcatca gcataggtgg cgtcgttgta
      241 gtgatggaaa gtattgtaag tttgaccaga aatatcgttg gtcgctgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccccctc agggacgtaa taagttgatc catcaattat caatgcatag
      421 aaaacatatt atgtttcgcg ctaacgatta tgctgcgtta cagacgaatg gttcaattat
      481 tctttcccct aagtccgaat gtggacatat ataatttatg gagaaaaaca cgcatcaaat
      541 tgataaaagg acgatatttc agcatgttgc agataatgtt atttttgatg catacgcgtt
      601 ataaatatca aaaacacaaa ccagataaat atcttatcaa tatacaacaa agtttgaatt
      661 attcaattta tagcagcaca accaaacgcc aaaaaaataa taaccaagag ctataaataa
      721 agattaaaaa tattttcagt gaatatgaaa ttattgctat ttctattaca aagatgtcct
      781 ggtctctctt tcattctctt cttgtcaaca tcgcgacaac tttcgtaaaa cactcctgcc
      841 ccgcttcagg tatactgtgc accttaattc cacaaacatc aggcagacca tgacagactt
      901 aatccaacgc cctcgtcgcc tgcgcaaatc tcctgcgctg cgcgctatgt ttgaagagac
      961 aacacttagc cttaacgacc tggtgttgcc gatctttgtt gaagaagaaa ttgacgacta
     1021 caaagccgtt gaagccatgc caggcgtgat gcgcattcca gagaaacatc tggcacgcga
     1081 aattgaacgc atcgccaacg ccggtatccg ttccgtaatg acttttggca tctctcacca
     1141 taccgatgaa accggcagcg acgcctggcg ggaagatgga ctggtggcgc gtatgtcgcg
     1201 catctgcaag cagactgtgc cagaaatgat cgtcatgtca gacacttgtt tctgtgaata
     1261 cacttctcac ggtcactgcg gtgtgctgtg cgagcatggc gtcgacaacg acgcgactct
     1321 ggaaaattta ggcaagcaag ccgtggttgc agctgctgca ggcgcagact tcatcgcccc
     1381 ttccgccgcg atggacggcc aggtacaggc gattcgtcag gcgctggacg ctgcaggctt
     1441 taaagatacg gcgattatgt cgtattcgac caagttcgcc tcttcctttt atggcccgtt
     1501 ccgtgaagct gccggaagcg cattaaaagg cgaccgcaaa agctatcaga tgaacccaat
     1561 gaaccgtcgc gaggcgattc gtgagtcact gctggatgaa gcccagggcg cagactgcct
     1621 gatggttaaa cctgctggag cgtacctcga catcgtgcgt gagctgcgtg aacgtactga
     1681 attgccgatt ggcgcgtatc aggtgagcgg tgagtacgcg atgattaagt tcgccgcgct
     1741 ggcgggtgct atagatgaag agaaagtcgt gctcgaaagc ttaggttcaa ttaagcgtgc
     1801 gggtgcggat ctgattttca gctactttgc gctggatttg gctgagaaga agattctgcg
     1861 ttaatttttt tcctctcaga aagggggagt taacctgttg aaagtacggt gaacgccccc
     1921 tcaccctaac cctctcccca agggggcgag ggaaccgtcc acactcgtat caccccgccc
     1981 gataaaacgg tttatcccca aggatcgtcg cccgatgcat aatccgccgc tgtggcaggt
     2041 aatcggcatt ggcatagtgc tgagtcacgc ggttatccca aatcgcaata tcattcggtt
     2101 gccagcgcca acgcacctga aactccggtt tagtgatatg ggcgaataaa aagcctaaca
     2161 aggcttcgct ctctttctcg ctgacatcga caattcgcgt agtaaagcct tcattcacaa
     2221 acagcgcctg tttaccgctc accggatgcg ttcgcaccac cggatgtagc aacggaggat
     2281 ttttcgcgac cgcctcgcac cagcgttgat gttcctcctc ggttttgcgg tatttgtatt
     2341 ccgggaatga tttacggaaa tcatgctccg cacgcagcca gctcagcagc tggcggaagg
     2401 gaacagagag cgcctcatag gccgcaatac cgctggttca gagcatatca ccgccggtcg
     2461 aaggtaactc tttagctgcc agaatcgccc ctgcgggtgg cgtttcaata aatgtcacat
     2521 cggtatgcca gttgtcgtta tccggcggat tatcgttatg ggtatccagc acgatgatct
     2581 cgtcaacccc ttcggcatga gggtaaacag ggtgaatatg cagttcgcca aaacgctggg
     2641 ccagcgcgcg ttgctgctgc ggcgtaatag cttgatcgcg cagaaatacc acctgatggc
     2701 gcagcaccgc atggtaaagc tgttcaaact gattatcgct taacgggcgc gtcaggtcgg
     2761 cacccgaaat ttgtgcgccg atatgcggcc ccagcggggt aatgctcaga cgttcaccca
     2821 ttgtacttct ccatgccagg gcgtcagacg gcgctgtaac gcgcgcagac ccagttctaa
     2881 aagaaaggcg ataatcgcaa tcaccgcgat ccccgccagt accacgtcag tcgcgagaaa
     2941 ttcacccgct gactgaacca taaatcctaa accgcgtgtc acggcaatca gctccgccgc
     3001 taccagcgta gaccagccca cacccagacc aatgcgtaat ccggtgagga tttccggcag
     3061 cgcgccgggc aaaatgacaa accacagcac ctgcgcacgg ctggcaccca gcgactgggc
     3121 ggcacgaatg cgaacctgct gcgcgctttt cacccctgcc agcgccgaca tcgccaccgg
     3181 cgcaaaaatc gctaaataga tcagtaagat cttcgaggtt tcaccaatac caaaccagat
     3241 caccatcagc ggcaaataag ccagcggtgg caccggacga taaagttcga ttaccggatc
     3301 cagaatgccg cgcaccgtcg ggcttagccc catggcgatc ccgaccggaa taccgatcac
     3361 caccgccgcg agtagcgcct gcacaatgcg cgtcaggctg gcagccagat gctgccacag
     3421 cgtggcgtcc ataaatccct gcggtccggc aatggtgagc agttttgcca gtacttgttg
     3481 cggcggcggc aaaaacagcg ggctaatcag ttgcagcgcc gccaccgtcc accatacagt
     3541 gagtaaaacc gctaacgtgc caatgcttaa ggtcacctga cgcgagagcg gccagcgcca
     3601 tttcagccgc cgcgaatgca gtttttcatt aatgagcaca ctcatgagaa cgcctcccgt
     3661 tgctcaaata cgcggcttaa aacatattcg cgcatggcga tgaattgtgg atcggacttg
     3721 atgctgcggc tcgactctcc cgcaacaaag cggcgagcaa agttgagcgg cagccgctcc
     3781 agcacacggc cagggccgga tgaaagcaga accagttcag tcgccataaa caccgcttct
     3841 tctatatcgt gggtaatcaa cagcacctgc ttgcccgtct cctgccagag tttcagcagc
     3901 agagtttgca tctggtcgcg ggtgaaggcg tccagcgcac caaacggttc atcgagtaat
     3961 aacagctggg gattcgccgc cagcgcgcgg gcaatcccca cccgctgacg ttgaccatcg
     4021 gaaagctgcc agatatagcg tttttctgcg ccttccagcc ccactttttt cagcatctgg
     4081 tgcgcgattt ccagtcgctg cattttctct atacctgcca attgcaggcc gaacgccacg
     4141 ttgtcctgta cattgcgcca cggtagcagc ccttcattct gaaaaactac gccacgctct
     4201 gctcccggtc cctcaatacg cttacccgcc agttgaatgc tgccatgctg ataaggcaca
     4261 aaaccggcaa tcagattcag cagggtggtt ttaccgcagc cggacggccc cagcaccacc
     4321 agtagctcgc cgctttccag cgtcaggttg atatcttcca gtgccggttt gccgccataa
     4381 tcggcataga gatgagagat ttgcagcatc tgcgcctcct tttattgcac gaagcgcgag
     4441 gtaacgtact ggctgtaatc attcgctaca gccgggacct tgccctgctc tttcaaaaac
     4501 tgcgcggtgt cgatgattgc tttgttcacc ggtccggtca gttctgccgt ttgttgctgc
     4561 ggcgtcagat aggtatttcc cttcaccagc cccggaacgt caccttcagg cacgccgctt
     4621 aaacgcgcca gtttgctgat gttttccggc tgtttcagcc acgcgtcagg gttagcaatg
     4681 tacggttgct gtgcatcgat ggcgcttttg gcgaacgctt tcacgacctc aggatgtttc
     4741 tcggcaaaat ctttgcgcac cacccagacg tccagcgttg gcgcacccca ctgcccgacc
     4801 tgttcagaat cggtcagcac cttgtcgtct ttttccaggg cgttaaccgc cggtgcccag
     4861 acataagcac catcaatatc accccgctgc catgcggcga taatcgcggg cggctgcagg
     4921 ttcacaatct ccactagccc tggtttaatc ccccagtgtt tcagcgccgc cagcaggctg
     4981 tagtgggtgg tggagataaa cggtacggca atgcgtttac caatcaaatc ttcgggtttg
     5041 ctgatagttt tctttaccac cagcgcttcg gagttaccca gttttgacgc cagcaagaag
     5101 acttcaatcg gcacctgttg gctggctgca accgctaatg ggctggatcc gaggttgccg
     5161 atttgcacgt cgccagaagc cagcgcccgc acgatgctgg ctccgctgtc aaacttacgc
     5221 cagtccacgc tcgccccgct ctctttagca aaggtgttgt cggcctgagc cactttcgcc
     5281 ggttcggctg aggtttgata cgccacggtg acgttcaccg cctgtgcctg aaaagcgatg
     5341 aatgccagtg cggcaagaag tgtgttatat gactaggtaa tttattcaag ataaccggga
     5401 atgggtaaaa aaagacggga tttgatggga ttcaataagt tatgcgccga ttgcattcaa
     5461 gataaaacga gttaaaaatg ctcaatgctt cgatatcgat aaaacattca agataggcgc
     5521 gccgtgtatt ttaattcttt taagactctt caaaaatgtc atcgtagcct ttaaaaacga
     5581 attttaaagg caccctaaag gcaggtcaat gaagttaacc cgccttcgcg tttacactct
     5641 ctgagagaat actagcttca atctcttcca aaatctctct gacttgctcg tcgggcatat
     5701 caacgagcat caacgctgcc aggcgtttac ggccctctag ttgcagtagc tcttgagttt
     5761 ccttatctaa caagcgtagc acagtctcaa cacctttcct tgcgagcatg tttgtaaggg
     5821 cttctcgttc ctcactggta agaaacgaca acatttctgt tagctgatcc ccttggctta
     5881 aatcactttg tgttttttgt gtttttggtg gattcccgtt accggttttt agccattcaa
     5941 gactaacacc ttcactttct gcaatgcgta ccaatatgct ttctcgaggt tctggtgtat
     6001 ttcccttttt aaaatagctt tgtaacgtat tgatatttat gccacaggct ctggcggcag
     6061 cacttcttga tggataccgc ctaataagat aagccatgcg ctccacgata ggttcttttg
     6121 cctgctccat aaaagtacct ctaggtattt ttctgtataa aggttctttt ccatctatct
     6181 aattgaatgg aaaggtaatt ttaaaaatcc aaataaaata caaaaaaata ccttgtattg
     6241 gtgctttttg tgatctgcga ttttatttac tcggatatcc acgcgaatat ccgcgcgggt
     6301 aattttgtag ggtaaaggaa agatgagaca aagagaagat agtggccgcg attggccttc
     6361 gctgatgatt cagcaagcac tcaaagataa tgggtggtct tcctttcggg agctttcact
     6421 ccatgccggt tatgctcagg attcactgaa agcggtagtt cgtacgccaa acaaaaaata
     6481 cgaacgtgtt atttctgacg ctataggtgt accgccagaa atcatctggc ccagccggta
     6541 tcgtaacgat ggatttatca ggaaggcgtc gtgatatgca gtcgtacgtt accgtaaatg
     6601 accttctcgg tgttcctgga atgccagcca ccgcgaaagg catccgacag gcgttacagc
     6661 gtttttctcg tgctcttggt gacgttagtc gccgtcgagc aggtacaaaa gccattgagt
     6721 accatatcga ttgcctgcct gaggtaaccc aaaaggcact gcgtgaacgc tatgtggaac
     6781 aactggtggc tacagagaac aacgtctccg aagtgaagac cgttaccagg acggcgcgta
     6841 accctgatgc tgttcaggca gttgaagcat atcggggttc tccgcaacta atggaagaac
     6901 gcctgaatgc cctgactgaa aatcagcgcc gggtgtctga agcaagagcg gcgctggcag
     6961 tggaagtgct gaagctggaa agcgccggta acctcgggcg actgaaagcc attaactttc
     7021 tggttgaaaa agcccgtaaa ggtgagttgc cggaacgact gcaacaggct gcgattaacg
     7081 ctaatgcaaa acgtggcact aatcgcacca tcagccgtga tccgctttac cagtgggtac
     7141 tgaaatacaa ccggtcgcag aacgccgcag aacgactgtt gttactggca ccaggaaaac
     7201 gcgacgaaat aaaaccggaa gaaattagct ggctgcctga atttctggcg cagtatcgtc
     7261 aggctaacgg cagacctatg tcggaagcct acgaggattt tgtcacggaa tggcaacgtc
     7321 gctatgcgga cgaaccgtac atgctggagg tgatgccttc ctatgatgtt gtccggtatg
     7381 ccatgaaaaa attgccggaa gtggtgaaac aaaaaggacg cgttaccggc agtgaatacc
     7441 gccagcttga gggattcacc cgccgcgact ggacagctat gccggtgaat tatgtctgga
     7501 ttggtgacgg ccacggtatg aagctgaaat gtgcgcatcc gatccacggg cgtccatttt
     7561 caccggaagt cacgtttgtt atcgacggtg gaacccggtt tgttgttggc tggagtcttg
     7621 atcttgcgga gaacgtgttt gctgttgccg gggcgataca gcacggaatt cgcaatcacg
     7681 gaaaaccctt cctgtattac tcggataatg gttccggtga aacggcagat atgctggata
     7741 aagagatcgt cgggattctg ccccgtctgg gtattaacca ccccaccggt attgccggaa
     7801 atccgcaggg acgcggcatc attgaacgtc tgaaccggac gctgccgatg cgtatagcca
     7861 gacggtatcg cacctatttt ggcaaggggg cagaccggga aacactgcgt gtactcaatc
     7921 gcgatctgcg ttcagcattt aatgccctgc aacaggacaa accgctgaat gcccgccaga
     7981 aatctgcaat gcgcgagctg ccgtcctggt cagaactgat tgaggctatt cgtgaaggag
     8041 tggaatggta caacaaccgc ccgcattctg aactgccgat gaaaccaaac gggaagcatt
     8101 acagcccggc tgagttcaga aaaaaacgcc tggcggaaga aaacaccgaa attgaatggc
     8161 tgagtgatct cgaactgcgc gacatgttct gcccgatggt ggagcgtccg gtaagacgct
     8221 gtgaaatcaa atggcttggg aatatttatt acgcgccgga gctgcgtgat gaacatggtc
     8281 gcaaagtgct tatcagctat gacattcatg atgcagagcg aattacagtg cgccgtaagg
     8341 atggcagttt tatttgtgag gcgatatgga acggcaataa acgcgccgcc ttcccggtca
     8401 gcgccgacta ttacaaacaa cagcagcgta ttaaaggaat gcacaaacgc gcagaagaaa
     8461 aaatccgtga tgccgtggat gaaggtattc aggttattga acaaaaaacg acagaaccct
     8521 ggctggataa tgtttatcga cccgttggca atgtgatgac cgttcagcaa caggaatatg
     8581 aagaggagca cgacgaagaa tccgaacgca atttcaggcg gggattacag aaac
//
LOCUS       DAJTYF010000143         8611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTYF010000143 DAJTYF010000000
VERSION     DAJTYF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.143"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..2386)
                     /gene="mdoH"
                     /locus_tag="OO590_003577"
     CDS             complement(<1..2386)
                     /gene="mdoH"
                     /locus_tag="OO590_003577"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6638797.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNR"
     gene            complement(2379..3914)
                     /gene="mdoG"
                     /locus_tag="OO590_003578"
     CDS             complement(2379..3914)
                     /gene="mdoG"
                     /locus_tag="OO590_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6638798.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4308..5465
                     /gene="mdoC"
                     /locus_tag="OO590_003579"
     CDS             4308..5465
                     /gene="mdoC"
                     /locus_tag="OO590_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6638799.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5473..6954)
                     /gene="clsC"
                     /locus_tag="OO590_003580"
                     /pseudo
     CDS             complement(5473..6954)
                     /gene="clsC"
                     /locus_tag="OO590_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
     gene            complement(6896..7429)
                     /gene="ymdB"
                     /locus_tag="OO590_003581"
     CDS             complement(6896..7429)
                     /gene="ymdB"
                     /locus_tag="OO590_003581"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6638800.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7524..7835)
                     /locus_tag="OO590_003582"
                     /pseudo
     CDS             complement(7524..7835)
                     /locus_tag="OO590_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(7956..8288)
                     /gene="csgC"
                     /locus_tag="OO590_003583"
     CDS             complement(7956..8288)
                     /gene="csgC"
                     /locus_tag="OO590_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6638801.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8347..>8611)
                     /gene="csgA"
                     /locus_tag="OO590_003584"
     CDS             complement(8347..>8611)
                     /gene="csgA"
                     /locus_tag="OO590_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR6638802.1"
                     /translation="NSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNG
                     KNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT         2153 a         2236 c         2039 g         2183 t
ORIGIN      
        1 cgcgattcag cttctctggc gtctcgttaa gcgcctgttc aacgtggcgg tcacgggcga
       61 tttccagcac cttactggcg cggtgacgcg ctgtcgccat tgcggttgcc agagcgttaa
      121 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      181 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      241 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      301 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      361 gccacgccat cccaacagcc cacactaacc ccaacagcag ctgtgagccg tggcgtttga
      421 acgcttcgcc ccatgaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      481 agccaaggaa cgcgctgaca acgaagaccg tatggaacag catgcgtacc ggagccagca
      541 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      601 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      661 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
      721 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgcact acctgcaatg
      781 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
      841 tcaggaacac cgcacggtga accgggtgca tacccttaac caggaacaga cggaagttca
      901 tcaggttacc gtggcaccag cggcggtcac gttttagctc atcaagcaag ttaggcggca
      961 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1021 tcaacgccgc ttctacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1081 gcagcggagc cagtgcgcag tgctcgataa atggtttcac gcggataatc gcgttatgtc
     1141 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaac agtggcccat
     1201 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1261 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caagcgcacc aggccgcata
     1321 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1381 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1441 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
     1501 gctctgcgac gcagatatcc gggttataac tgtcactcag aatgtagaca tcaaagtgtt
     1561 tggcattccc ggtggctttt actgattccc acgttgcacg caagccagca aaaacacggt
     1621 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1681 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     1741 agcccatcaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaaaagga
     1801 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     1861 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     1921 tataccaggt cgcgacaaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     1981 tggtaccaac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2041 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2101 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttaagctgat
     2161 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2221 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2281 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgct gctttctcgc
     2341 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2401 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2461 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2521 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2581 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2641 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     2701 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     2761 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     2821 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     2881 ttgccccact cccctttcgg agtcacccat gcgcttggac ggagatcgta acgatcatcg
     2941 agatcttcaa agcgggagaa atcacgacct cgctgcaaca gaccaaagcc tcgcgggttt
     3001 tccatggaga agctgctgac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3061 tcgccattac cagcatggat agacagaccg ttagagtcgt gcaactccgg acgatagtta
     3121 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3181 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3241 cctggcataa ccacgaattt gtaagcacct gtcgcacgcg gcgagtcaag caatgcataa
     3301 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3361 aattcttcac ccgatggcaa agcggtatca attgctaggc cgcgggcaga aaggccataa
     3421 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3481 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3541 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3601 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3661 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     3721 ttaaactgga tctgctgata gtccgcgtat ttcatgtcgc ggaaaacgga gggcaagttg
     3781 cttttaggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     3841 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     3901 cgcattttca tcatttgtag tttatgtttc ataattagta agcacttccc cctttgtgtg
     3961 cttatatcga tccgatctat tttaatggat aaccagccat tccgacaact gaatcccggt
     4021 atcgttcagc acgctggctc atggattaag cacctgattg caaccgtttt cactttccgt
     4081 gcttctccta aagacagaag catcgacttc gtgtagggtt tgccagcaaa tttatttatc
     4141 aaccgtctta cagacgcttc cattctggtt agtggtagcg atgatgctat gcataaaaac
     4201 cttgaagcct gcgcctggcg cggtcaggtc ggtgtcgcta tggcaacaag cctaaaggca
     4261 taacccgaca gggataagac gaaaaatccg agattagtta accatatatg aatccagtac
     4321 ccgcgcaacg tgaatatttc ctcgactcca tccgcgcctg gctgatgttg ttagggatcc
     4381 cttttcatat ttctttaatc tattcgagcc atacatggca tgtgaatagc gccgaaccgt
     4441 cattgtggct gacccttttt aatgacttca tccactcgtt ccgcatgcag gtatttttcg
     4501 ttatatccgg atacttttcc tacatgcttt ttttacgcta tcccttgaaa aaatggtgga
     4561 aagtacgtgt cgaacgtgta ggtatcccga tgttaacagc catcccccta ctgacattac
     4621 cgcaatttat tatgctgcaa tatgtcaaag ggaaagcgga aagttggcct gggctatcat
     4681 tgtatgacaa atataatacg ttggcctggg aattaatatc acacctgtgg tttttactgg
     4741 tgttagtagt catgacgacg ctgtgtgtat ggatatttaa gcgcatcaga aataatttag
     4801 aaaattctga taaaacgaat aaaaaattct cgatggtaaa actatcggtg atttttttat
     4861 gcctcggcat cggctatgcg gtaataagaa gaacgatttt tattgtgtat ccacccattc
     4921 tgagtaatgg catgttcaat tttattgtca tgcaaacgct attttattta ccgttcttta
     4981 tcctcggcgc actggctttc attttccctc atcttaaagc cttgtttacc acgccgtctc
     5041 gtggctgtac cctcgctgca gcattggcgt ttgtcgctta tttactcaac cagcgctatg
     5101 gcagtggcga tgcctggatg tacgaaaccg agtcggtgat caccatggtc ctcggcctgt
     5161 ggatggtgaa tgtggtcttc tcctttggcc accgtttact taacttccag tcagcgcggg
     5221 tgacttattt tgttaacgca tcgctgttta tctatctggt tcaccacccg ttaacgctgt
     5281 ttttcggcgc atacattaca ccgcacatca cctccaactg gcttggtttt ctctgtggcc
     5341 tgatattcgt agtagggatt gcgataattc tgtatgaaat tcatttacgc atcccgttac
     5401 tgaagttttt gttctctggt aaaccggttg ttaagcgtga gaacgataaa gcaccagccc
     5461 gttaagccac atttacaata accattccac gggcaatatc gacgccagtc tgaccataac
     5521 ccgcttccag aaactggtgg cgggttcttt tttgagaata acctctttct ttgcatgacg
     5581 atcaacccag ttgatccgtc cccacctgtc cagacggagc tgccaggccg catcgtactg
     5641 gctctgaata aagcgtttat caattaactg tgccagcgtt tcgctctcta tcacgaagcc
     5701 catttcagta ttgagcaatg ttgaacgcgg atcgaaattg aaagagccga taaacaccgt
     5761 tttaccatcg atgctaaaag ttttagcatg caggctggca ccggaattac cggttatgcc
     5821 gcgatcgtgt aacgtactgc tttgttcacg cgtcggcttg agttcatata attccacgcc
     5881 atagcggagc aattttttgc gccagcgcgc gtatccggca tggacgacag caacatcgtt
     5941 agcggcaaga gaattggtta ggatcgcaat ctttacccct tttctcacca tccgtaagag
     6001 ttgcgccaca cctgcgcgtg tcggtacaaa ataggaagag ataatatcga tgcgttcact
     6061 gggtgagccc atgatatcga acaggcgctg cggtaacagt gaatgccgtt ttgccttgcc
     6121 ctcccctttc gccggatcat cacttaataa acgtgtcttc gcccagataa gcggcaacgt
     6181 tccatcaacc agatgattta taaatggact ggattccatt ttgcgtaaat aacgatgcgt
     6241 catggcatcg ttatgccagg aggcgggtaa ctcgatgcga tccgccattt caccttccgg
     6301 gacatccagc acctgctgta agggtgaaac cgatttgcaa taccagtagc gggcgaaatc
     6361 atcggcaacg tcctctacca cggggcctat tgccatgaca tctaaatccg aaaaaagtcg
     6421 ctcttcccct gcactaaaat aggcatcacc aatatttagt cctcccacta gggtaaccac
     6481 gccatcgaca gtgaaacttt tattgtgcat ccggcgattg agacgggaaa agtcggtgat
     6541 atatccaagc ggacgtagca agcgaaacga gaaaggatta aaaagccgga cttcaatgcg
     6601 tggatgactg tcaagcaagc gtaaaatgtc gtcaagtccg ggcgtattgt tgtcgtccag
     6661 caacaaacgg acgcgaacgc cacgctttgc tgcggctaac agggcggaaa acagtaaccg
     6721 ccccgacata tcgtcctacc agatgtaata ctgaacatcc agcgtatgct ctgccatttc
     6781 ggccagacga taccgggcgg caaacgcatc cagacttttc tccagaggga aaagaccaca
     6841 ctgaccggga tgttgcgaac acagtggcag caccgcgctc gccagccggg gcaaatcatt
     6901 catctccttg ttgggtaagg agtctttcgt agaggtgggc gttttcttca tcataacaga
     6961 caaagtatac ctgttcgggt aaagcgtgac gggtaataaa ttctgaaacg gttttaactg
     7021 cgatttcagc cgctgccgca cgagggtaac cataaacccc agtactgatt gcaggaaaag
     7081 ccactgacgt atagctgttt gccgccacca gtcgtaggct attcagatag gcatcctgca
     7141 aaagctggtc ttcgttttgt tcaccaccac gccagactgg cccgacggtg tgtactacgg
     7201 ctttagcggg aagatcgcct gcaagcgtaa taacggcatg ccccgtaggg caatcgccct
     7261 gctgttgcct gacttttaaa caggcatcca gcagggccgg acccgctgcg cgatgaatgg
     7321 ccccatcgac gccgccgcct cccattaatg acggattagc cgcattcaca atcacatcaa
     7381 cggccagttt ggtaatatca ccctgcacaa catgaatacg cgttttcata acgcctcctt
     7441 acaccttctt gtttcttaag tgtacagcag gctttttggt taaatcgcat ttttctgctc
     7501 agcaacgtga gttgttcaga aacttagtta tattcgatgt tcattacagc cagctttttc
     7561 tgtttatcaa gatactgcac ttttactgag gctatttgcg cgtttttgac attgccgctc
     7621 attaacgctt tgctggagta ggttttgccc ggaatagaac cattctatgg gcagttcatt
     7681 tctacgggtg atgactgggt gctgacgtca catgccggtt ccacaatttg gccataaaaa
     7741 tggatcacgc ccccttgcgc ggtgcttcct gcaatggcaa taaaaggaaa aaacaaactg
     7801 ccgagcataa gagagtctaa aaaaggtctg aacatttatt ctatttcctc aatgattagt
     7861 catccttgag ggttgtgtta tccatacttt caggttggct gattcgcagc agaccattct
     7921 ctccagattc atcttatgct cgatatttca acaaattaag acttttctga agagggcgtc
     7981 cattgttgtg ataaatgaag tgcctgtcca tcagaaacag taacaactat tttcacccga
     8041 tcttccgggg aaatatttaa actcaacttc gtcaaagcaa tgggttgatt agcaggcaat
     8101 gagagggtct tttcttgctt cgtctgactt tgccctgaac tgccttcgcg cagggacaat
     8161 atttgtactc tgcacagaca agattgagta agagtaactt caggaataat ggtgtacata
     8221 tccccttgct gggtcgtatt aaaggttatc tggctggaaa gtgccgcaag gagtaataac
     8281 gcattcatat tcttctcccg aaaaaaaaca gggcttgcgc cctgtttctg taatacaaat
     8341 gatgtattaa tactgatgag cggtcgcgtt gttaccaaag ccaacctgag tcacgttgac
     8401 ggaggagttc gatgcagtct ggtcaactgc agcaccgttg ccaccaccga actgtttaac
     8461 agtcatttca gaatttttgc cgttccactg atcaagagtg gcgctgttac cgaagccacg
     8521 ttgggtcaga tcgattgagc tgtcatctga gccctgacca acatctgcac cattaccacc
     8581 gccatgctgg gtaatagtca agtcagagtt a
//
LOCUS       DAJTYF010000144         8496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTYF010000144 DAJTYF010000000
VERSION     DAJTYF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.144"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..920)
                     /gene="rspA"
                     /locus_tag="OO590_003585"
     CDS             complement(<1..920)
                     /gene="rspA"
                     /locus_tag="OO590_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6638803.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
     gene            962..1303
                     /locus_tag="OO590_003586"
     CDS             962..1303
                     /locus_tag="OO590_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR6638804.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1369..1898
                     /gene="speG"
                     /locus_tag="OO590_003587"
                     /pseudo
     CDS             1369..1898
                     /gene="speG"
                     /locus_tag="OO590_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1901..2611)
                     /locus_tag="OO590_003588"
     CDS             complement(1901..2611)
                     /locus_tag="OO590_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6638805.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2719..3024
                     /locus_tag="OO590_003589"
     CDS             2719..3024
                     /locus_tag="OO590_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6638806.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3223..3546
                     /locus_tag="OO590_003590"
                     /pseudo
     CDS             3223..3546
                     /locus_tag="OO590_003590"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4102..4701
                     /locus_tag="OO590_003591"
                     /pseudo
     CDS             4102..4701
                     /locus_tag="OO590_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(5293..6282)
                     /locus_tag="OO590_003592"
     CDS             complement(5293..6282)
                     /locus_tag="OO590_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638807.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA"
     gene            complement(6357..6866)
                     /locus_tag="OO590_003593"
     CDS             complement(6357..6866)
                     /locus_tag="OO590_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638808.1"
                     /translation="MSSKASALFNNNDWKLHVTDINLYENTVSLDGQSYPLSFAIKTL
                     IPGYLSGLPATSRESMELLEALAEAGVTIGNFFSNDLMTAYQRRQQNKRAEAERIAKE
                     QRIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADIE
                     SGGNWMDSL"
     gene            complement(6968..7189)
                     /locus_tag="OO590_003594"
     CDS             complement(6968..7189)
                     /locus_tag="OO590_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638809.1"
                     /translation="MTTKQHIDPRTPIGKATLRYRGLPTRRLLSMLGMGVEDPERPFY
                     SRDELIDLLVDRDLNNQLRRAFAKLDATH"
     gene            complement(7436..8281)
                     /locus_tag="OO590_003595"
     CDS             complement(7436..8281)
                     /locus_tag="OO590_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638810.1"
                     /translation="MNTNEVSVINLDQLVSMSSYDFLTKVINPARVEAGEKPVRNNDF
                     VSRIEDELDGQNVAYEIFVSFGQDVKSYTLNTDQLLLVGMRESKAVRRKVLAYIRQKE
                     QEVLELQAQQLQVAQTKAICYAPGAIVLDSKLDSKSGATPTEVIKALDLKLTSSKMLT
                     ASVLTGYLKTLCRPNPFYTKRVTEFGTTDIDLSTGRPRAIFNKKGETNGYYRLQPQPN
                     DKEPTIKILRKGYDWLKDNWKDIAHDMRTSNIKVVRRLVDSHLNGKATKSQKAAAGKG
                     SDYGF"
BASE COUNT         2126 a         2133 c         2130 g         2107 t
ORIGIN      
        1 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
       61 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
      121 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
      181 gctggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
      241 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
      301 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
      361 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
      421 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
      481 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
      541 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
      601 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
      661 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
      721 atacggtgcg catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc
      781 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
      841 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac
      901 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
      961 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     1021 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     1081 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     1141 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     1201 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     1261 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     1321 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     1381 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     1441 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     1501 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     1561 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     1621 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     1681 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     1741 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     1801 aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa
     1861 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     1921 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     1981 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     2041 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     2101 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     2161 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     2221 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     2281 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     2341 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     2401 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     2461 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     2521 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     2581 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     2641 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     2701 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     2761 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     2821 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     2881 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     2941 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     3001 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     3061 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     3121 taataattct taacccaaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa
     3181 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     3241 atggtgggca tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcgctg
     3301 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     3361 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     3421 cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac cggcagcgat
     3481 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     3541 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     3601 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     3661 gtcgaacagt atggcaatga agctgtatat attcagtacc cttcggggat tgttggcggc
     3721 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     3781 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     3841 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     3901 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     3961 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4021 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4081 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     4141 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     4201 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     4261 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     4321 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     4381 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     4441 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     4501 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     4561 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     4621 ttcatctgga actacaagga cttgaagcgg ttactcgtta gagtcacgag agaccttagt
     4681 aggcctgagg tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc
     4741 attagacggg atgtctagac ggctggtcag gcccccggtg gcctctttgt caggactcag
     4801 ggtggcaccg gggaacattg gtgtcttaat tgtattgaat gaggtctcta acttttggtc
     4861 caaaactcat cgttagctgc tcgccagctc acagccgaac ccgaactgta agcacgacca
     4921 ccagcaccag cttcagcagg aagacgacaa gatagacttt atgcaggtcc atgcgtcctc
     4981 ctgtgtctat tgtaccactg cactagataa ctagtacaaa ataaagtcca gaccgaaggc
     5041 ccagaccata gttaattaat cacattgata atccaccacc tgaagtcctg ccaacattca
     5101 gtccagcctt acccatgcta ggagtcttaa ccggggtgtc gttctgtgtc tccaccactg
     5161 gtgccgcatc aggctctaca ggaagctcca cagcaggcgc agggaccgga ggtgatacgg
     5221 atgtaccacc accagcggag aagcccttag cgatggaccg gaggccttta ctaatgctct
     5281 tacccattgc tgttacgctt cggtcttggt gaacttcagg cgtttgacta caggcaggac
     5341 tgcggttgca gcgccagtgt ccagcttaac tgtcgtagca gcttcagcga caacgaagac
     5401 cttaccgagg atttccaagt cgtgggactg agagtcgcca tcacgtgaaa ggacgaccgt
     5461 aatattctca ccgtcgtgac ggaagcagcc gaagctcagg gccgtgaagc gtttagccag
     5521 catgaacaca cctttgtcct caccgttgta atacgcgatg tcggtaccca gcatatccac
     5581 gatagcgtct tcaccagaga agccctgttt agcagccaga cgggacagcg ctttcagttg
     5641 ggactcatgg gcgatgatgc cgtaatcaga cacttcagag ccgaaagagg actcggtgtt
     5701 ctcaaacaga gcgtccatga tagcgtctgc cttagtggtc aggcttccag tcaggactgg
     5761 cagctctacg gtcttcagca gcttggagga ctccagaacg tcaaccatgc gagcgttgat
     5821 ggtacggacc acattctcac agatgttcat agcgtcctgc tggagtgtgc catgagtgaa
     5881 accagtggag gacttggcgt cccacagttt agtttgctcc gagatgagga ttgcctgttc
     5941 gataggttcg acgacaatct cctgaccaga cggaacggtc acgacttcag gtcgctcacc
     6001 accgcgtaca gggacgacca cagagcggtc agacagggtg tgggcgatga ttggggtagg
     6061 caggctattg cgggtaggca ggacttctac agggaacgga atgttatcct gagcgtcttt
     6121 aagtacaccg ttcagcatag ccttaaccgt cttagctaac tggagggcat agacctgtgg
     6181 gtcagcgtta gttggcagac cttcgttaac ctgaatgttg tcggtgtcgg tgatacgtac
     6241 tggctcaatg ccgaactgat agctatcttt aatcatggtc ataataatca atctcctaaa
     6301 ttagttttag atactgtggg tcccgacatc aggtcaggac cacttgtgtg tctgtcttag
     6361 aggctgtcca tccagttacc gcctgactca atgtcagcga caatagcggc acgggagcga
     6421 ccagcggaat gggtagcact gcggaggttc tcaccatagg tccggcgctc agctttgacc
     6481 tgttcgcggc gctgtagctc tttctgccac tctgcatcgg tcatgttctc ttcacgcata
     6541 cgctcagcct gaatgcgctg ctctttagcg atacgctcag cctcggctcg cttgttctgc
     6601 tggcgacgtt ggtaggcggt catcaggtca ttgctgaaga agtttccaat ggtcacgcca
     6661 gcttcagcca gagcttccag taattccatg gactcgcggc tggtggcagg cagtcctgac
     6721 aggtagcccg gaatcagggt cttaatagcg aaggacagtg ggtaggactg accgtccaga
     6781 ctcacagtgt tttcgtagag attgatgtcg gtgacatgga gtttccagtc attgttgttg
     6841 aataacgcac tagctttgct agacatagta attactcctc ttgagagttg agactatcgg
     6901 taccgcagac acagttatgc ctacgggtct ccccgcatcg gtcatctcaa aaggtttaaa
     6961 ttaggggtta gtgagtggcg tccagcttag cgaacgctcg gcgtaactga ttgttcaggt
     7021 cacggtcgac cagcaggtct atcagttcgt ctcgactata gaaaggacgc tcagggtcct
     7081 ctacgcccat ccccagcatt gacaggaggc gacgggtagg cagaccacgg taacggagag
     7141 tagcctttcc gatgggtgtc cgtgggtcga tatgttgttt agttgtcatt gttagaagtc
     7201 ctcgttagac caatcatcca cgtcaaattc actggtgata ggagcttgga agttaggtag
     7261 accctctggt atctctagag gcagtcgctc gtcgactctc agtgccatct cttcgtgtat
     7321 atgccgaagc agagaagtca gtaagatagg gtccgcagga tgctcagggc cgtgtctctc
     7381 aatgaaacat atggttgtcg ctattgactc cataatcagt tcatcagtga gtcgcttaga
     7441 acccatagtc agaccccttg cccgctgctg ctttctggga ctttgtagcc ttaccattca
     7501 ggtgagaatc caccagacga cggacaacct tgatattgga tgtgcgcatg tcatgagcga
     7561 tatccttcca gttatccttc agccagtcat aacccttgcg tagaatcttg atggtaggct
     7621 ctttgtcgtt aggctgtggc tgtagacgat agtagccgtt ggtctcacct ttcttattga
     7681 agatggctcg tggacgtccg gtcgataggt ctatatccgt agtgccaaac tcagtaacac
     7741 gcttggtgta gaatgggtta ggacgacaca gggtcttcag atatccagtc aggacgctag
     7801 ctgtcagcat cttagagctg gtcaacttaa ggtccagagc tttaataacc tcggtaggcg
     7861 tggcgcctga cttggagtcc agcttggagt cgaggacgat agcaccgggc gcatagcaga
     7921 tagctttggt ctgtgcgacc tgaagttgct gggcctgaag ttcgaggacc tcttgctcct
     7981 tctggcgaat ataggctagg accttacgac gcacagcttt agactcacgc atcccgacca
     8041 gcagaagctg gtcagtgttg agggtatagc ttttaacgtc ctgcccgaag cttacgaaaa
     8101 tttcgtaggc tacattttga ccgtccagct catcttcaat acgggacaca aagtcgttgt
     8161 tacggacagg cttctcccca gcctccacac gagccgggtt gatgacctta gtcaagaaat
     8221 cgtagctact catagacaca agctggtcaa ggttgatgac gctaacttcg ttggtattca
     8281 taaaggatgc tcctttggta aagcactttc tgtctccatg tggagtccgc acctattcaa
     8341 tgcatccaaa cggacccacc aaaaagtgct taacgaaagt gaatagggtt aatgttgatt
     8401 agtgttggtc tggagtgacc atgttctcca tgtgcatata aggcgagacc gcatgtcagg
     8461 acgagctgta aggaccccag atggtgagcg gtgata
//
LOCUS       DAJTYF010000145         8391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTYF010000145 DAJTYF010000000
VERSION     DAJTYF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.145"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(127..918)
                     /gene="aadA1"
                     /locus_tag="OO590_003596"
     CDS             complement(127..918)
                     /gene="aadA1"
                     /locus_tag="OO590_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6638811.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(1011..1484)
                     /locus_tag="OO590_003597"
     CDS             complement(1011..1484)
                     /locus_tag="OO590_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6638812.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1644..2657
                     /gene="intI1"
                     /locus_tag="OO590_003598"
     CDS             1644..2657
                     /gene="intI1"
                     /locus_tag="OO590_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6638813.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2860..3210
                     /locus_tag="OO590_003599"
     CDS             2860..3210
                     /locus_tag="OO590_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6638814.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3336..3896
                     /locus_tag="OO590_003600"
     CDS             3336..3896
                     /locus_tag="OO590_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6638815.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3899..6865
                     /locus_tag="OO590_003601"
     CDS             3899..6865
                     /locus_tag="OO590_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6638816.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6932..7309
                     /locus_tag="OO590_003602"
     CDS             6932..7309
                     /locus_tag="OO590_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6638817.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7510..8169)
                     /locus_tag="OO590_003603"
     CDS             complement(7510..8169)
                     /locus_tag="OO590_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6638818.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1860 a         2447 c         2276 g         1808 t
ORIGIN      
        1 tcaaaaacac catcatacac taaatcagta agttggcagc atcaccgcaa ttatgtgctt
       61 agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg aacgaattgt
      121 tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgaa caaattcttc
      181 caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc
      241 ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac
      301 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
      361 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
      421 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
      481 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
      541 cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag
      601 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc
      661 tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat
      721 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
      781 actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt
      841 cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc
      901 gatcaccgct tccctcatga tgtttaacgc ctggcacagc ggatcgcaaa cctggcgcgg
      961 cttttggtac aaaaggcgtg acaggtttgc gaatccgttg ctgccacttg ttaacccttt
     1021 tgccagattt ggtaactata atttatgtta gaggcgaagt cttgggtaaa aactggccta
     1081 aaattgctgg ggatttcagg aaagtaaaca tcaccttccg gctcgatgtc tattgtagat
     1141 atatgtagtg tatctacttg atcgatcagg cttttgtata tctccccacc acctgaaaca
     1201 atgacatgat ccgttatttt ctttaggttg gttaaagcat ctttaattga tggaaagatc
     1261 actacgttct cattgtcaga tgtaaaactt gaacgtgtta cgaccgcata ctttcggttg
     1321 ggtaatgctc ccattgattc aaaagtcttg cgtccaacca acagccattg gttataggta
     1381 atagctttaa acaggagctg ttcacctttg gcactccatg gaatatcagg gccattcccg
     1441 ataactccat tcttcgatat agctaccatt agtgatagtt tcacaattct tcctcagagg
     1501 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca
     1561 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
     1621 caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
     1681 cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa
     1741 ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc
     1801 cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca
     1861 aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg
     1921 tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct
     1981 tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc
     2041 atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg gatcagtgag ggtttgcaac
     2101 tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat cgtgcgggag ggcaagggct
     2161 ccaaggatcg ggccttgatg ttacccgaga gcttggcacc cagcctgcgc gagcagctgt
     2221 cgcgtgcacg ggcatggtgg ctgaaggacc aggccgaggg ccgcagcggc gttgcgcttc
     2281 ccgacgccct tgagcggaag tatccgcgcg ccgggcattc ctggccgtgg ttctgggttt
     2341 ttgcgcagca cacgcattcg accgatccac ggagcggtgt cgtgcgtcgc catcacatgt
     2401 atgaccagac ctttcagcgc gccttcaaac gtgccgtaga acaagcaggc atcacgaagc
     2461 ccgccacacc gcacaccctc cgccactcgt tcgcgacggc cttgctccgc agcggttacg
     2521 acattcgaac cgtgcaggat ctgctcggcc attccgacgt ctctacgacg atgatttaca
     2581 cgcatgtgct gaaagttggc ggtgccggag tgcgctcacc gcttgatgcg ctgccgcccc
     2641 tcactagtga gaggtagggc agcgcaagtc aatcctggcg gattcactac ccctgcgcga
     2701 aggccatcgg tgccgcatcg aacggccggt tgcggaaagt cctccctgcg tccgctgatg
     2761 gccggcagca gcccgtcgtt gcctgatgga tccaacccct ccgctgctat agtgcagtcg
     2821 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacaa tggaggtggt agccgagggt
     2881 gtggaaacac ccgactgcct tgcgtggttg cggcaggcgg gttgcgacac ggtgcagggt
     2941 ttcctgttcg ccaggccgat gccggcggcg gccttcgtcg gcttcgtcaa ccaatggagg
     3001 aacaccacca tgaacgccaa tgaaccgagc accagttgct gcgtgtgctg caaggaaatc
     3061 ccgctcgatg ccgccttcac gccggaaggg gccgagtacg tggagcattt ctgcgggctg
     3121 gagtgctatc agcgcttcca ggcgcgggcc agcactgcga ccgaaaccag cgtcaaaccg
     3181 gacgcttgtg attcgccgcc gtcaggttga ggcataccct aacctgatgt cagatgccat
     3241 gtgtaaattg cgtcaggata ggattgaatt ttgaatttat tgacatatct cgttgaaggt
     3301 catagagtct tccctgacat tttgcaggga attccatgac tggacagcgc attgggtata
     3361 tcagggtcag caccttcgac cagaacccgg aacggcaact ggaaggcgtc aaggttgatc
     3421 gcgcttttag cgacaaggca tccggcaagg atgtcaagcg tccgcaactg gaagcgctga
     3481 taagcttcgc ccgcaccggc gacaccgtgg tggtgcatag catggatcgc ctggcgcgca
     3541 atctcgatga tttgcgccgg atcgtgcaaa cgctgacaca acgcggcgtg catatcgaat
     3601 tcgtcaagga acacctcagt tttactggcg aagactctcc gatggcgaac ctgatgctct
     3661 cggtgatggg cgcgttcgcc gagttcgagc gcgccctgat ccgcgagcgt cagcgcgagg
     3721 gtattgcgct cgccaagcaa cgcggggctt accgtggcag gaagaaatcc ctgtcgtctg
     3781 agcgtattgc cgaactgcgc caacgtgtcg aggctggcga gcaaaagacc aagcttgctc
     3841 gtgaattcgg aatcagtcgc gaaaccctgt atcaatactt gagaacggat cagtaaatat
     3901 gccacgtcgt tccatcctgt ccgccgccga gcgggaaagc ctgctggcgt tgccggactc
     3961 caaggacgac ctgatccgac attacacatt caacgatacc gacctctcga tcatccgaca
     4021 gcggcgcggg ccagccaatc ggctgggctt cgcggtgcag ctctgttacc tgcgctttcc
     4081 cggcgtcatc ctgggcgtcg atgaactacc gttcccgccc ttgttgaagc tggtcgccga
     4141 ccagctcaag gtcggcgtcg aaagctggaa cgagtacggc cagcgggagc agacccggcg
     4201 cgagcacctg agcgagctgc aaaccgtgtt cggtttccgg cccttcacca tgagccatta
     4261 ccggcaggcc gtccagatgc tgaccgagct ggcgatgcaa accgacaaag gcatcgtgct
     4321 ggccagcgcc ttgatcgggc acctgcggcg gcagtcggtc attctgcccg ccctcaacgc
     4381 cgtcgagcgg gcgagtgccg aggcgatcac ccgtgctaac cggcgcatct acgacgcctt
     4441 ggccgaacca ctggcggacg cgcatcgccg ccgcctcgac gatctgctca agcgccggga
     4501 caacggcaag acgacctggt tggcttggtt gcgccagtct ccggccaagc caaattcgcg
     4561 gcatatgctg gaacacatcg aacgcctcaa ggcatggcag gcactcgatc tgcctaccgg
     4621 catcgagcgg ctggttcacc agaaccgcct gctcaagatt gcccgcgagg gcggccagat
     4681 gacacccgcc gacctggcca aattcgagcc gcaacggcgc tacgccactc tcgtggcgct
     4741 ggccaccgag ggcatggcca ccgtcaccga cgaaatcatc gacctgcacg accgcatcct
     4801 gggtaagctg tttaacgctg ccaagaataa gcatcagcag cagttccagg cgtcaggcaa
     4861 ggccatcaac gccaaggtac gtctgtacgg gcgcatcggt caggcgctga tcgacgccaa
     4921 gcaatcaggc cgcgatgcgt ttgccgccat cgaggccgtc atgtcctggg attcctttgc
     4981 cgagagcgtc accgaggcgc agaagctcgc gcaacccgat gacttcgatt tcctgcatcg
     5041 catcggcgag agctacgcca ccctgcgccg ctatgcaccg gaattccttg ccgtgctcaa
     5101 gctgcgggcc gcgcccgccg ccaaaaacgt gcttgatgcc attgaggtgc tgcgcggcat
     5161 gaacaccgac aacgcccgca agctgccagc cgatgcaccg accggcttca tcaagccgcg
     5221 ctggcagaaa ctggtgatga ccgacgccgg catcgaccgg cgctactacg aactgtgcgc
     5281 gctgtccgag ttgaagaact ccctgcgctc gggcgacatc tgggtgcagg gttcacgcca
     5341 gttcaaggac ttcgaggact acctggtacc gcccgagaag ttcaccagcc tcaagcagtc
     5401 cagcgaattg ccgctggccg tggccaccga ctgcgaacaa tatctgcatg agcggctgac
     5461 gctgctggaa gcacaacttg ccaccgtcaa ccgcatggcg gcagccaacg acctgccgga
     5521 tgccatcatc accgagtcgg gcttgaagat cacgccgctg gatgcggcgg tgcccgacac
     5581 cgcgcaggcg ctgatagacc agacagccat ggtcctgccg cacgtcaaga tcaccgaact
     5641 gctgctcgaa gtcgatgagt ggacgggctt cacccggcac ttcacgcact tgaaatcggg
     5701 cgatctggcc aaggacaaga acctgttgtt gaccacgatc ctggccgacg cgatcaacct
     5761 gggcctgacc aagatggccg agtcctgccc cggcacgacc tacgcgaagc tcgcttggct
     5821 gcaagcctgg catacccgcg acgaaacgta ctcgacagcg ttggctgaac tggtcaacgc
     5881 tcagtttcgg catccctttg ccgggcactg gggcgatggc accacatcat catcggacgg
     5941 acagaatttc cgaaccgcta gcaaggcaaa gagcacgggg cacatcaacc caaaatatgg
     6001 cagcagccca ggacggactt tctacaccca catctccgac caatacgcgc cattccacac
     6061 caaggtggtc aatgtcggcc tgcgcgactc aacctacgtg ctcgacggcc tgctgtacca
     6121 cgaatccgac ctgcggatcg aggagcacta caccgacacg gcgggcttca ccgatcacgt
     6181 cttcgccctg atgcacctct tgggcttccg cttcgcgccg cgcatccgcg acctgggcga
     6241 caccaagctc tacatcccga agggcgatgc cgcctatgac gcgctcaagc cgatgatcgg
     6301 cggcacgctc aacatcaagc acgtccgcgc ccattgggac gaaatcctgc ggctggccac
     6361 ctcgatcaag cagggcacgg tgacggcctc gctgatgctc aggaaactcg gcagctaccc
     6421 gcgccagaac ggcttggccg tcgcgctgcg cgagttgggc cgcatcgagc gcacgctgtt
     6481 catcctcgac tggctgcaaa gcgtcgagct acgccgccgc gtgcatgccg ggctgaacaa
     6541 gggcgaggcg cgcaatgcgc tggcccgtgc cgtgttcttc aaccgccttg gtgaaatccg
     6601 tgaccgcagt ttcgagcagc agcgctaccg ggccagcggc ctcaacctgg tgacggcggc
     6661 catcgtgctg tggaacacgg tctacctgga gcgtgcggcg catgcgttgc gcggcaatgg
     6721 tcatgccgtc gatgactcgc tattgcagta cctgtcgcca ctcggctggg agcacatcaa
     6781 cctgaccggt gattacctat ggcgcagcag cgccaagatc ggcgcgggga agttcaggcc
     6841 gctacggcct ctgcaaccgg cttagcgtgc tttattttcc gttttctgag acgaccccta
     6901 ttatggcgaa ggtatcattc aggaagcgtt gatgaaggat tatctctgta aaaagttatt
     6961 caaccggctt tccggtaccc tggtgatcag agcgcgctgc ggcaataaca ttactggcct
     7021 ggcatgttgc aatattcttt atccctcgcc ccgatacagc ggtcagctgc atattaaaga
     7081 gctgtatgtt tctcagtgcg atcgaaataa gggcacgggt aaagcgataa tgcgctttat
     7141 agcccggctt gcgcttgaac aggaatgcct tagccttagc tggaacgctg aaaaatccaa
     7201 ccccggcgct aaccgttttt atcaggctct gggaggcaga ataaatgatc atatcgtcaa
     7261 ttattacctc cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg
     7321 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
     7381 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
     7441 ccgcttatta tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc
     7501 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     7561 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     7621 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     7681 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     7741 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     7801 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     7861 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     7921 caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc
     7981 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     8041 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     8101 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     8161 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     8221 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
     8281 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
     8341 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt c
//
LOCUS       DAJTYF010000146         8350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTYF010000146 DAJTYF010000000
VERSION     DAJTYF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.146"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..158
                     /locus_tag="OO590_003604"
     CDS             <1..158
                     /locus_tag="OO590_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638819.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO590_003605"
     CDS             complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO590_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6638820.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO590_003606"
     CDS             complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO590_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6638821.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            874..1293
                     /locus_tag="OO590_003607"
     CDS             874..1293
                     /locus_tag="OO590_003607"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6638822.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            1280..6145
                     /gene="traI"
                     /locus_tag="OO590_003608"
     CDS             1280..6145
                     /gene="traI"
                     /locus_tag="OO590_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCR6638823.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQYNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISSEQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQVETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIA
                     PGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPR
                     LEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEA
                     REQTLIVTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWE
                     NNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRV
                     GTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQAYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPT
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQA
                     VREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVR
                     DLQKEKTPGGD"
     gene            6165..6911
                     /gene="traX"
                     /locus_tag="OO590_003609"
     CDS             6165..6911
                     /gene="traX"
                     /locus_tag="OO590_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6638824.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6966..7526
                     /locus_tag="OO590_003610"
     CDS             6966..7526
                     /locus_tag="OO590_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6638825.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPWLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7657..7869
                     /locus_tag="OO590_003611"
     CDS             7657..7869
                     /locus_tag="OO590_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6638826.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8113..>8350
                     /locus_tag="OO590_003612"
     CDS             8113..>8350
                     /locus_tag="OO590_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6638827.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1916 a         2095 c         2673 g         1666 t
ORIGIN      
        1 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
       61 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      121 aacgcgggga ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt
      181 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      241 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac
      301 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      361 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      421 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt
      481 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      541 tggtatcgag cataaacttc agcatcagaa tgactccctt tcctgcatgc cgggctgttc
      601 cctgttatcc ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      661 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      721 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      781 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      841 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
      901 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
      961 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1021 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1081 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1141 atctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1201 gtggattttg ctgtccgtca tcacccgtgt ggaggcactg gcctccacac gggtgatgac
     1261 ggacggacag tcagaaacgg tgctgaccgg taatctggtg atggcactgt ttaaccacga
     1321 caccagtcgc gatcaggaac cacagttaca cacgcatgcg gtggtgacta atgttacgca
     1381 gtataacggc gaatggaaga cactgagcag tgacaaagtg gggaaaacgg ggttcattga
     1441 gaatgtgtac gctaatcaga ttgcctttgg caggctctac cgggaaaaac tgaaagagca
     1501 ggttgaggcg ctgggctatg aaactgaagt ggtcggtaag cacggtatgt gggaaatgcc
     1561 gggtgtaccg gtggaggcct tttccggacg ctcacagact atccgggagg ccgtcgggga
     1621 agacgcctcg ctgaaatccc gggatgtggc ggcgctggat acgcgtaaat ccaaacagca
     1681 cgtcgatcct gaagtcagaa tggccgagtg gatgcagacg ctgaaggaaa ccgggttcga
     1741 catccgggca tatcgtgacg cagcggaaca acgggcatat acccgcacgc agacacccgg
     1801 acccgcttca caggacgggc cggatgtgca gcaggcggtg acacaggcca ttgccggatt
     1861 aagtgaacgc aaagtgcagt ttatgtatac ggacctgctg gccaggacgg tcggcatact
     1921 gccgccggaa aatggtgtga ttgaacgggc acgcgccggt atcgatgagg ccatcagcag
     1981 tgagcagctt atccccctcg atcgtgagaa ggggctgttc acgtccggga ttcatatgct
     2041 cgatgagctg tcagtccggg cactcagtcg tgacatcatg aaacagaacc gggtgaccgt
     2101 acatccggag aaaagtgtcc cccggacggc cggttacagc gatgccgtca gtgttctggc
     2161 acaggaccgt ccgtcgctgg ccattgtgtc cgggcagggc ggtgcagccg ggcagcgtga
     2221 gcgggtggct gaactggtca tgatggcccg ggagcagggg cgggaggtgc agattatcgc
     2281 tgctgaccgt cgctcgcaga tgaacctgaa gcaggatgaa cggctgtccg gtgaactgat
     2341 aaccggacgt cgtcagttgc tggaaggcat ggccttcacg ccgggcagta ccgttatcgt
     2401 cgaccagggc gaaaaactct ccctgaaaga gacgttaacc ctgctggatg gtgccgcacg
     2461 tcataacgta caggtcctga taaccgacag cgggcagcga accggtacag gcagtgcact
     2521 gatggccatg aaggatgccg gggtgaacac atatcgctgg caggggggag aacagcgacc
     2581 ggccaccatc atcagtgaac cggaccgtaa tgtccgctat gcccggctgg ccggagattt
     2641 tgcggccagc gtgaaagccg gagaagagag cgtggcacag gtcagcggag tacgggaaca
     2701 ggccatactg acacaggcca ttcgcagtga gctgaaaaca cagggcgtgc tcggacaccc
     2761 ggaggtaacc atgaccgccc tgtcaccggt ctggctggac agccggagcc gttatctgcg
     2821 ggatatgtac cgcccgggaa tggtgatgga gcagtggaac ccggagacac gcagtcatga
     2881 ccgctatgtg acagaacgtg tgacggcgca gagtcacagc ctgaccctgc ggaatgcgca
     2941 ggtcgaaacg caagtggtgc gtatttcttc cctggacagc agctggtcgc tgttccggcc
     3001 ggaaaaaatg ccggtggcag acggtgagcg tctgagggtg acagggaaaa ttcccggact
     3061 ccgcgtctcc ggcggtgacc gcctgcaggt ggcatccgtc agtgaagatg cgatgacggt
     3121 tgttgtgccg ggacgggctg aaccggccac cctgcctgtg agcgattcac cgttcacggc
     3181 actgaagctg gagaacggct gggtggaaac gcccgggcat tccgtcagcg acagtgcgac
     3241 ggtttttgcc tccgtcacac agatggcaat ggacaatgcc accctgaatg gtctggcccg
     3301 cagtggtcgt gatgtccggc tgtattcctc actggatgaa acccgtactg cggaaaaact
     3361 tgcccgccat ccctccttta cggtggtttc tgagcagata aaggcgcgtg ccggtgagac
     3421 attgctggaa accgctatca gtctgcagaa agccgggctt cacacgccgg cacagcaggc
     3481 cattcatctg gcccttcctg tgctggaaag taaaaacctg gccttcagca tggtggacct
     3541 gctgacagag gcgaagtcgt ttgctgcaga aggaaccggt tttgctgacc tgggagggga
     3601 aatcaatgcg cagataaaac ggggtgattt actgtatgtg gatgtggcaa aaggctatgg
     3661 cacaggcctg ctggtttccc gtgcgtcgta tgaggcagaa aagagcattc ttcgccatat
     3721 tctcgaaggt aaggaggcgg tcacgccgct gatggagaga gtacctggcg aactcatgga
     3781 gaaactgaca tcaggacagc gcgccgccac ccgtatgata ctggaaacgt ctgaccgttt
     3841 cacggtggtg cagggctatg ccggtgtggg taagaccaca cagttccggg cggtgatgtc
     3901 agccgtgaac atgctgccgg agagtgaacg tccccgtgtc gtggggctgg ggcccacaca
     3961 ccgtgcggtc ggggagatgc gcagcgccgg cgtggatgca cagacactgg cgtcctttct
     4021 gcatgacacg cagcagcgca gcggagaaac gccggatttc agcaacacgc tgttccttct
     4081 cgatgagagc tctatggtgg gcaataccga catggcacgg gcatacgccc tgattgcggc
     4141 cggtggcggt cgggctgtgg ccagcggtga cacggaccag ctgcaggcca tcgcgcccgg
     4201 tcagcctttc cgtctccagc agacgcgcag tgctgccgat gtggtcatca tgaaggagat
     4261 tgtgcgtcag acgccggaac tgcgggaggc ggtatacagc ctgattaacc gggatgtgga
     4321 aagggcgctg tccgggcttg agcgtgtgaa accgtctcag gtgccacgtc tggagggcgc
     4381 atgggcaccg gagcactccg tgacggagtt cagtcacagc caggaagcga aactggcaga
     4441 agcgcagcag aaggcgatgc tgaaaggcga ggcttttccg gatgtcccca tgacactgta
     4501 tgaagccatt gtccgcgact ataccggcag gacaccggaa gcacgggagc agacgctgat
     4561 tgtcacgcac ctgaatgagg accggcgcgt actgaacagc atgattcatg atgcacggga
     4621 aaaggccgga gaactgggga aggtgcaggt catggtgcct gtcctgaaca cagcgaatat
     4681 acgcgacggg gagctgcgtc gtctctccac ctgggagaat aacccggatg cccttgccct
     4741 ggtggataat gtgtatcacc ggattgccgg tatcagcaag gatgacgggc tgataaccct
     4801 gcaggatgcg gaaggtaaca cgcggctgat ttcgccccgg gaggcggtgg ctgaaggcgt
     4861 cacgctgtac accccggaca ccatccgggt ggggaccggt gacaggatac gcttcacgaa
     4921 gagtgaccgg gagcgcggtt atgtggccaa cagcgtctgg acggtgacag cggtttccgg
     4981 tgacagtgtc acactttctg acggtcagca gacccgtgtg attcgccccg gtcaggagcg
     5041 ggcagagcaa catattgacc tggcctatgc catcaccgcc cacggtgcac agggggcgag
     5101 tgaaaccttt gccatcgcgc ttgaaggcac ggaaggtaac cggaaactga tggccggctt
     5161 tgagtcagcc tacgtggccc tgtcgcgtat gaagcagcat gtgcaggcgt acaccgataa
     5221 ccgtcagggc tggacggatg ccatcaacaa tgccgtacag aaaggaactg cccacgatgt
     5281 gtttgagccg aaaccggacc gggaggtcat gaatgcagag cggctgttca gtacggcgcg
     5341 ggagctgcgg gacgtggcgg caggccgtgc cgttctccgt caggcggggc tggccggggg
     5401 agacagtcct acacggttta ttgctccggg acgtaaatat ccgcaaccgt atgtggcact
     5461 gccggcgttt gaccgtaacg gcaagtccgc cggtatctgg ctgaacccgc tgaccacgga
     5521 tgacggaaac gggctgcggg gattcagcgg tgaaggtcgg gtaaaaggca gcggggatgc
     5581 gcagttcgtg gccctgcagg gcagccgtaa cggagaaagc ctgctggctg ataatatgca
     5641 ggatggcgtc cggattgccc gtgataatcc tgacagtggt gtggtggtaa gaatcgccgg
     5701 tgaaggccgt ccgtggaatc ccggtgccat caccggtggc cgcgtgtggg gggatatccc
     5761 ggacaacagc gtccagccgg gagccggaaa tggcgaaccg gtcacggcag aggtactggc
     5821 acagcggcag gctgaagagg ccatccgccg tgaaacggaa cgtcgcgcag atgaaattgt
     5881 ccgtaaaatg gcagagaaca aacctgacct gccggacggc aaaacagagc aggctgtcag
     5941 ggaaattgcc ggtcaggagc gtgaccgggc tgccataact gaacgggaag ccgcgctgcc
     6001 ggagagtgta ctgcgtgaac cacaacgggt gcgggaagcg gtccgggaag ttgcccggga
     6061 aaatttgctg caggagcgtc tgcagcagat ggagcgggat atggttcgtg acctgcagaa
     6121 agagaaaacc ccgggcggag actgatacag gaagacaaac tctgatgaca acggataaca
     6181 cgaacacgac acgtaacgat tcactggctg cacggaccga tacctggttg cagtcatttc
     6241 tggtctggtc acccggacag cgggatatca tcaaaacggt ggcactggtg ctgatggtac
     6301 tggaccacat caatctgata ttccagctga agcaggaatg gatgtttctg gccgggcgag
     6361 gagcctttcc gctttttgcc ctggtgtggg ggctgaatct gtcccgtcat gcgcatatcc
     6421 ggcaaccggc cattaaccgg ctgtggggat ggggaattat tgcccagttc gcatattatc
     6481 tggcaggctt tccttggtat gaggggaata tcctgtttgc ctttgcagtg gcggcacagg
     6541 tgctgacgtg gtgtgaaacg cgcagtgggt ggcgtacagc agcggccata ctcctgatgg
     6601 ccctgtgggg gcctttgtcc ggcaccagtt acggtattgc cgggttgctg atgctggcag
     6661 tcagcaaccg gctgtaccgg gcggaagaca gagcggaacg tctggcgctg gtcgcctgcc
     6721 tgctggctgt tattccggcg cttaaccttg ccaccagtga tgcagcggcg gtggccggtc
     6781 ttgtgatgac ggtgctgacc gttggtctgg tgttgtgtgc cggaaaatca ttaccccgtt
     6841 tctggcccgg agactttttc ccgacgttct atgcctgtca tctggctgtg ctgggcgttc
     6901 tggcgctgtg acgggtgtgg tatctttggc cgcaagagga tgattcgtca gaggcagaac
     6961 acagcatgat agagcagaag cgaccggtac tgacactgaa gcggaagaca gagggaacag
     7021 cgcctgtccg cagccggaaa accatcatca atgtcaccac gccaccaaag tggaaggtga
     7081 aaaagcagaa gctggccgag aaagcggccc gtgaagcaga gctggcggca aaaaaagcgc
     7141 aggccagaca ggcgctgtcc atttatctga acctgcccac gctggatgag gctgtgaata
     7201 ccctgaagcc ctggtggccg ggattatttg acggtgacac gccctggctt ctggcctgcg
     7261 gtatccggga cgtgttactg gaagacgtgg cgcagcggaa tatcccgctc tcgcataaaa
     7321 aactgcgcag ggcgctgaag gccatcaccc gttcagaaag ctatctgtgt gccatgaaag
     7381 ccggtgcctg ccggtatgac acggaagggt atgtgacgga gcatatttct caggaggagg
     7441 aagcgtatgc gggagcgcgt ctggcgaaaa ttcgtcgcca gaaccggata aaggcagaac
     7501 ttcaggccgt gcttgatgag aaataaaaaa agcctccccc agagagagga ggcagggaaa
     7561 taaggcttaa aaggaaataa cttccgcttc cgaaaggaag ccagtccact gaattattta
     7621 tcacaataat actgacaact aaagggggac aagaaaatga atggattcag aaacagttca
     7681 cggaacggcc aggtctggcg ttaccagcgt gccggcgggc gggccgttat tctggaagtc
     7741 agtggacgct ggatggaagc ggcagaagca tggcgacggg ctgcctgtgt tgcgcctagg
     7801 acagactggc agcagtttgc ccggaaaagg gctgaacact gccaccggcg ctgccgggga
     7861 agggtgtaac gaaaaaagca gccttcatta ctctaggagt cagaaggctg aaaggagttt
     7921 atgagatttg cttcttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca
     7981 gggtcttttc cgcattaagt cgatttttta tattttgtga ttaatttcac aaaataaggt
     8041 gttattcaat gtgtgctgca ctattcaggg gggctgaaat acaggtggca tttatctgat
     8101 atggagaata acatgaggaa atatattcca ctggtattat ttatcttttc atggccggta
     8161 ttgtctgcag atattcatgg acgggttgtt cgtgtactgg acggtgatac catcgaggtc
     8221 atggactcac tgaaggcggt gcggatcagg ctggtaaata ttgatgcacc ggaaaagaaa
     8281 caggattatg gccggtggtc aacagacatg atgaaatcac tggtcgcagg aaaaacggtt
     8341 accgtcacat
//
LOCUS       DAJTYF010000147         8323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTYF010000147 DAJTYF010000000
VERSION     DAJTYF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.147"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            336..3185
                     /locus_tag="OO590_003613"
     CDS             336..3185
                     /locus_tag="OO590_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6638828.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3285..5204)
                     /gene="dhaR"
                     /locus_tag="OO590_003614"
     CDS             complement(3285..5204)
                     /gene="dhaR"
                     /locus_tag="OO590_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6638829.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5432..6502
                     /gene="dhaK"
                     /locus_tag="OO590_003615"
     CDS             5432..6502
                     /gene="dhaK"
                     /locus_tag="OO590_003615"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6638830.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDVPVHTPALNWGK"
     gene            6513..7145
                     /gene="dhaL"
                     /locus_tag="OO590_003616"
     CDS             6513..7145
                     /gene="dhaL"
                     /locus_tag="OO590_003616"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6638831.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7156..>8323
                     /gene="dhaM"
                     /locus_tag="OO590_003617"
     CDS             7156..>8323
                     /gene="dhaM"
                     /locus_tag="OO590_003617"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6638832.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEE"
BASE COUNT         2174 a         2069 c         2076 g         2004 t
ORIGIN      
        1 atcccataac taattaggat tattacaaaa aatcttcacc aaatttttta accgctgttt
       61 gttgtaatat ttttgccata ttcatagtaa cgaattggaa taagtaacga caacttaggg
      121 aataactcga tataaactca cctttgtaag gggggtgtta aatatatatt aattcctgtg
      181 ttcctgattt tgcccggtaa tagagaatta ttctcagaga agctcatcat gattgcacca
      241 tacagatatc atgatgataa gaggatatta ttcattgact taaacaaaat gacaaaatcc
      301 atagacccat agcctgggta ctatatagga gtggtatggg catcaaacaa cacaatggga
      361 ataccaaagc cgatcgtctc gctgaattaa aaatccgttc gccctcaatt caactgataa
      421 aatttggcgc tattggtttg aatgcaatta tcttttcccc cctgctgata gctgctgata
      481 caggaagtca atatggcacc aatattacta ttaatgatgg tgacagaatt acaggagata
      541 ccgccgatcc atcaggaaac ctctatggtg taatgacccc agcaggaaac acgcctggca
      601 atatcaacct gggtaatgat gtcaccgtca atgtcaacga cgcctctgga tatgcaaaag
      661 gaatcattat tcagggcaaa aacagctccc tgacagctaa ccgactcaca gtagatgttg
      721 ttggtcaaac ctctgccatc ggcattaact taattggtga ctatacccat gctgacttag
      781 gcacaggcag caccattaag agtaacgatg acggcatcat tattgggcat agctcaacac
      841 taacagccac tcaattcacc attgaaaact cgaacggtat aggcctaacc atcaatgact
      901 atggcaccag tgtcgatctt ggaagcggaa gtaaaatcaa gaccgatgga agtacaggtg
      961 tttatatcgg tggtctcaac ggcaataacg ccaatggtgc tgcgcgtttt acggcgacag
     1021 acctgacaat cgatgttcag ggctacagcg ccatggggat aaacgtacag aaaaactctg
     1081 ttgtcgatct cggaacaaac agtaccatta aaaccaatgg cgataatgct cacggcctct
     1141 ggagctttgg ccaggttagc gcgaatgcac tcactgttga tgtaactgga gccgcggcca
     1201 atggcgtcga agttcgtggt ggtacaacca ctatcggtgc agatagccat atttcttccg
     1261 cgcagggcgg tggcctcgtc accagtggtt cagacgcgac aatcaatttt tctggcacgg
     1321 cagcgcaacg aaacagcatc ttttccggcg gttcttatgg tgcctcggcc cagacggcaa
     1381 cggctgttat caacatgcaa aataccgata ttacggttga tcgtaatggc agtctggcgc
     1441 tgggtttgtg ggcgctcagc ggcggtagaa taaccggaga cagtttggct atcaccggcg
     1501 cggcaggagc cagagggatt tatgccatga ccaacagcca gatcgacctc acgagcgatc
     1561 tggtcattga tatgagtaca cccgaccaga tggccatcgc aacgcaacat gacgatggtt
     1621 atgccaccag ccgcatcaac gcctcgggtc gtatgcttat caacggtagc gttctttcca
     1681 aaggtgggct aatcaatctg gatatgcacc ctgggtcggt ttggacaggt tcctccctca
     1741 gcgataatgt caatggcggg aaactggacg ttgcaatgaa taacagcgtc tggaacgtaa
     1801 caagtaattc taatctcgac acgctggcgc tgagccattc aactgtcgat tttgccagcc
     1861 acgggtcaac tgccggcaca tttaccacat taaacgtaga gaacctgagc ggtaacagta
     1921 cctttattat gcgtgctgat gttgttggcg agggtaatgg cgttaataat agaggggatt
     1981 tattgaatat cagcgggagt agtgctggta atcacgtatt ggctatccgc aaccagggca
     2041 gcgaggccac aacgggaaat gaagttctga cagtggtaaa aaccactgac ggcgcggcct
     2101 cgttcagcgc gtcttctcag gttgagttgg ggggatatct gtacgatgtg cgtaaaaatg
     2161 gcactaactg ggagctttac gcttccggga cagttccgga accgactcct aatcctgaac
     2221 ccacaccagc tcccgctcag cctcccatag tcaaccccga tcctacgcct aaacccacca
     2281 cgaccgcaga tgctggcggc aattatctca atgtcggtta cttattgaac tatgttgaaa
     2341 accgtacgct gatgcaacgg atgggtgact tgcgaaatca gagtaaagac ggtaatatct
     2401 ggttgcgcag ttatggggga agcctggact cctttgccag tggcaaactg agcggctttg
     2461 acatgggtta cagcggtatc cagtttggtg gggataaacg tctctctgat gtaatgccgt
     2521 tgtatgtcgg tctgtatatt gactcaacac atgcatcgcc ggactatagc ggaggcgacg
     2581 gtaccgcacg ttcagactac atgggaatgt acgccagtta catggcacaa aacggttttt
     2641 acagcgatct cgttataaaa gcatcgcgcc agaaaaatag tttccacgta ctggacagtc
     2701 agaacaactg cgttaacgcc aacggcactg cgaatggaat gagcatctcc ctggaagccg
     2761 ggcagaggtt caacctgtcc cctactggtt atgggttcta tatagagccg caaacccagc
     2821 ttacatacag ccaccagaat gagatggcta tgaaggcgag taatggcctc aatatacatc
     2881 tgaatcacta cgaatcgctg ctggggcgtg ccagcatgat actggggtat gacatcaccg
     2941 caggcaacag ccagctgaat gtctatgtga agactggcgc tatccgcgag ttttcagggg
     3001 ataccgaata tctgttgaac gactcccggg agaagtacag tttcaaaggt aatggctgga
     3061 ataacggcgt gggagtcagt gcacagtata acaaacagca cacattctat ctcgaagcgg
     3121 attacacgca gggtaacctc tttgatcaga agcaagtcaa cggaggatat cgcttcagct
     3181 tttaagtgcg ggatagacaa gcccttaaac gatgctgaaa attgttagcg tgcgccggaa
     3241 tgaaatacag atcactctga tgtatcagaa atcgtttctg tctttcatac ccggcgctta
     3301 aactgccctg catcaatgcc atgttgcttc attttccgcc acagcgtagt gcggccgatc
     3361 ccaagtaaag ccgacatttc ctgaatgcga ccgcctgtga cctgggctgc gttaataatt
     3421 gcctcttttt caacttccgc aaatgacaga ctggtggaaa ggcgggtggc gctgacatca
     3481 tctgtcgcct gctcggtaaa cagatgttca ggtaaatcac tgacgcgaat gcgcccgtta
     3541 tcactactca gagcaagatt ctcgatgacg ctgtaaagtt caaaatcgtt gcctggccat
     3601 gcacaagaaa ctaggcgagc gagggcatcg tcatcaattt tcagccgcgt agagaagcgt
     3661 ttttcaagac tgcgtaattt gttattcacc agcgccggaa tgctgccacg ccgcatacgc
     3721 agaggcggga tggtaatttc aaatgcatgc agcgcgtaat acagctggcg actaaaacga
     3781 ttttgttcca ccagcattgc gaggtccgcg gtcgttgttg caatcacttt gacatcaatt
     3841 ggtattaaac gccgcgcatc cagtcgcgtg ataaccccct gcttgataac ctgcagcaaa
     3901 gcagactgta actccaccgc cagatattca atcttttcaa gaaacagcgt gccgccgtgt
     3961 gccagttcca gccgactcag acggccattt tcattgtccg tgcgatcgcc accaataaat
     4021 tcttccgcca gcgcagcatc accatataac tcacaattga cggcgatata aggacctgca
     4081 gcacgctcgc tttcattatg aattgcctga cttagcagtg ccttgcccac gccctcttct
     4141 ccacaaagca ggacaggaaa gctactgcgc gccgcctggc gaccaaaatg aatcaagcgg
     4201 cgggtttgcg gatcgtcctg tggcatatga gcgaaggtat ggctgacttt tcctaattga
     4261 ctggtcatta actgccgcat ctgttccaca ggatggagca acaaaataaa gctggttccc
     4321 tgcgtttcta ttatcggttt aagggttatc accgcatcaa taaactggtg ctggctttca
     4381 aaggttgctt ctacgtgttt gagcggatgt gcctgtttta ttgcttgttg caatacggcg
     4441 ggtaacgtta agagttcagt gattgcccgt ccctgacttg ccgtcgcgtc aaggcgcaag
     4501 acccgcgccg cctgggcatt aataaattgc aaattaccct gctcgtccca gctaatcacg
     4561 ccatcatcca tactttctaa cagggcatta agttgattta aatgacggtt agtttcagcg
     4621 agcaaactgt ccgtcagcag taaattaccg acctcgcggg cgattgccaa cgtcaacggc
     4681 aaatcagctg cggtagtttg ctcaaccgga cacgccagcg ctattgttcc cgtcaatcgg
     4741 cccttgctgt caaacaacgg cgttgcacaa aaggcccagt tccagagtgc ctgtttgaaa
     4801 tgttgatcgg ccatcgtttt cacggcctga ccagagatag ccgctaacga tagcgcacaa
     4861 gtaccaataa ttccctcggc gcaatacgtg ccgtcattga atcccagtgc acttagctgc
     4921 tgcaaggttt gcggatcgcc attacggctg agaatgcagg cggtttcatc gaggataaac
     4981 agcgcacact ctcgcggtgc catatattcc caggcatctt ccagcgcagc ctgcccgagc
     5041 gtcagcatcg ctttcttacg ccgataaata gaagcaaatg tcacgccctg ggcctgatgt
     5101 ggtacgttcc atgtctcccg tttcatcagc ttattgcatc gctcccagga ggttgcaatt
     5161 aagggagata tgccccgacc atcgttgtta aaagcgccac tcatatcccg catcccttat
     5221 gacaaaaggt gttccggctc gcaattttaa gtattaatac acgtaaaagt ttcaatatgg
     5281 aacatttatt aaagcatatg tttcactatg aaacataatt tgggctaatt ttcttttccc
     5341 ttgcctgatg cacaatggat tcatcggcag tcagtggtcg ccgtgtcgtt gaacatcatc
     5401 catgccctac cgtaattgct ggcgcaaaat aatgaaaaaa ttgatcaatg atgtgcaaga
     5461 cgtactggac gaacaactgg caggactggc gaaagcgcat ccatcgctga cactgcatca
     5521 ggatccggtg tatgtcaccc gagctgatgc ccctgttgca ggaaaagtcg ccctgctgtc
     5581 gggtggcggc agcggacacg agccgatgca ctgtggttat atcggtcagg ggatgctttc
     5641 gggggcctgt ccgggcgaaa ttttcacctc accgacgccc gataaaatct ttgaatgctc
     5701 catgcaaatt gatggcggcg aaggtgtact gttgattatc aaaaattaca ccggcgatat
     5761 tcttaacttt gaaacagcga ccgagttact gcacgatagc ggcgtaaaag tgaccactgt
     5821 ggtcattgat gacgacgttg cggtaaaaga cagtctttat accgccgggc gacgcggcgt
     5881 tgccaacacc gtattaattg aaaaactcgt aggcgcagcg gcggagcgtg gcgactcact
     5941 ggacgcctgt gcggaactgg ggcgtaagtt gaataatcaa ggccactcaa taggtatcgc
     6001 tctcggtgcc tgtaccgttc ctgccgcggg caaaccttct tttacccttg cggataatga
     6061 gatggagttt ggcgtcggca ttcatggtga gccaggtatt gaccgccgcc ctttctcttc
     6121 ccttgatcaa accgtcgatg aaatgttcga caccctgctg gaacatggct cataccatcg
     6181 cactttgcgt ttctgggatt atcaacaagg cagctggcag gaagaaccac aaaccaaaca
     6241 accgctccag tctggcgatc gggtgattgc actggttaac aatcttggcg caactccgct
     6301 ttctgagctg tacggcgtct ataaccgcct gaccacacgt tgccagcaag cgggattgac
     6361 tatcgaacgt aatttaattg gcgcgtactg cacctcactg gatatgaccg gtttctcaat
     6421 caccttactg aaagttgatg acgaaacgct ggctctctgg gacgtcccgg tccacacccc
     6481 ggcccttaac tggggtaaat aaggagaaag caatgtcact gagcagaact caaattgtta
     6541 actggctcac tcgttgtggc gatattttca acaccgagag cgagtatctt accgggctgg
     6601 atcgcgagat tggcgacgct gaccacgggc taaatatgaa ccgaggcttt agcaaagtgg
     6661 tggaaaaact ccctgctatc gcagataaag atatcggttt cattctcaaa aataccggta
     6721 tgacgctgct ttccagcgtc ggtggtgcca gtggtccgct gttcggtacc ttctttatcc
     6781 gcgccgcaca ggcgacccag gcacggcaaa gcctgacact ggaagagctt tatcagatgt
     6841 tccgcgatgg cgcggacggc gtaatcagtc gcgggaaagc cgaacctggc gataaaacca
     6901 tgtgtgatgt gtgggtgccg gtggtggaat cgttacgtca gtccagcgag caaaatctct
     6961 ctgttccggt ggcgctcgaa gctgccagta gcatcgccga atccgcggca caaagtacga
     7021 ttacgatgca agcccgcaaa ggccgcgcca gttatctcgg tgaacgcagt attggtcacc
     7081 aggatcccgg cgcgacctcg gtgatgttta tgatgcaaat gttggcgtta gccgcaaaag
     7141 agtaaggaat tggtgatggt aaacctggtc atagtttcac atagcagccg actgggagaa
     7201 ggtgtcggtg aattagcccg tcagatgtta atgagtgata gttgtaaaat cgccattgcc
     7261 gcgggaattg acgatccaca aaatcccatt ggtaccgatg ccgtcaaagt gatggaggcc
     7321 atcgaatctg ttgctgatgc cgaccatgtg ctggtcatga tggatatggg tagcgcatta
     7381 ttgagtgctg aaactgcgct ggaattgctg gctcccgaga tcgccgcaaa agtacgtttg
     7441 tgtgctgcgc cgttggtcga aggtacactg gcagcaacgg tcagcgcggc ctcgggggcg
     7501 gatatcgaca aagttatctt tgacgctatg catgcgctgg aagccaaacg tgaacaactg
     7561 ggtttaccgt cctccgacac tgaaatctct gacacatgtc ctccgtacga tgaagaagcc
     7621 cgttctctgg cggtggtcat aaaaaaccgt aacggcctgc atgtacgtcc ggcctcccgg
     7681 ctggtttata ccttatcgac atttaatgcc gatatgttgc tggaaaaaaa cggcaaatgc
     7741 gtcacaccag agagtattaa ccagattgcg ttactacaag ttcgctataa cgatacgctg
     7801 cgcctgattg cgaaagggcc agaagctgaa gaggcactga tcgctttccg tcagctggct
     7861 gaagataact ttggtgaaac ggaggaagtc gctccaccta ctctgcgtcc cgttctgcct
     7921 gtttcgggta aagcctttta ttatcaacca gttttatgta cggtacaggc aaaatcaacc
     7981 ctgaccgcgg aagaagaaca agatcgatta cgccaggcta ttgacttcac gttattagat
     8041 ctgatgacgt taacagcgaa agcagaagcc agcgggcttg acgatattgc cgcaatcttt
     8101 tctggtcacc atacactgtt aggtgatccg gaactgctgg cggcggcaag cgaactcctt
     8161 cagcatgaac attgcacggc agaatatgcc tggcagcaag ttcttaaaga acttagccag
     8221 caataccagc aactggatga tgaatatcta caagctcgct atattgatgt ggacgatctt
     8281 ctgcatcgca ccctggtcca cctgacccaa acgaaagaag aac
//
LOCUS       DAJTYF010000148         8193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTYF010000148 DAJTYF010000000
VERSION     DAJTYF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.148"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..671)
                     /gene="chbR"
                     /locus_tag="OO590_003618"
     CDS             complement(<1..671)
                     /gene="chbR"
                     /locus_tag="OO590_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR6638833.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIIN"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OO590_003619"
                     /pseudo
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OO590_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OO590_003620"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OO590_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OO590_003621"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OO590_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6638834.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OO590_003622"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OO590_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6638835.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="OO590_003623"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="OO590_003623"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6638836.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINLLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="OO590_003624"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="OO590_003624"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6638837.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OO590_003625"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OO590_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6638838.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREISTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OO590_003626"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OO590_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6638839.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="OO590_003627"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="OO590_003627"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6638840.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2152 a         2053 c         1893 g         2095 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatacta tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttaca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tgcggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttaacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctggcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatctgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattcg acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtggaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca aaacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgctg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc cctccctcgc
     8161 gctggaacgg ataaaccgaa ccgagattca gta
//
LOCUS       DAJTYF010000149         8162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTYF010000149 DAJTYF010000000
VERSION     DAJTYF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.149"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(66..605)
                     /locus_tag="OO590_003628"
     CDS             complement(66..605)
                     /locus_tag="OO590_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6638841.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(670..1218)
                     /gene="fimA"
                     /locus_tag="OO590_003629"
     CDS             complement(670..1218)
                     /gene="fimA"
                     /locus_tag="OO590_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6638842.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1699..2295)
                     /gene="fimE"
                     /locus_tag="OO590_003630"
     CDS             complement(1699..2295)
                     /gene="fimE"
                     /locus_tag="OO590_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6638843.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2773..3374)
                     /gene="fimB"
                     /locus_tag="OO590_003631"
                     /pseudo
     CDS             complement(2773..3374)
                     /gene="fimB"
                     /locus_tag="OO590_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            4828..5544
                     /gene="nanC"
                     /locus_tag="OO590_003632"
     CDS             4828..5544
                     /gene="nanC"
                     /locus_tag="OO590_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6638844.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5564..6670
                     /gene="nanM"
                     /locus_tag="OO590_003633"
     CDS             5564..6670
                     /gene="nanM"
                     /locus_tag="OO590_003633"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6638845.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNRWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKEEYYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6735..7715
                     /locus_tag="OO590_003634"
     CDS             6735..7715
                     /locus_tag="OO590_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6638846.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2422 a         1745 c         1699 g         2296 t
ORIGIN      
        1 ttccttacat tgacgttttt attactcact gtcctgttcc tgttatcaca ttatctactg
       61 aacaattact gataggttaa agagaaccag gcctgggcat tggcgatgcc gccagtaacc
      121 cgacgcccgg tagcacgata tttggcgatg aaatgtagcg aagtagagcc tgagtaaagc
      181 cgtttccagt ttgctggagg acgattaatc ggtacgaggt ttccttcatc atcaaacaac
      241 gctacaccaa tattggaggc tatccctggc ccctctccca cggagagcac atccggattt
      301 ttaccatccg cgacaccgtg aaacgccaca cccacacgtt cactcaccac cgtgctacat
      361 tcccgtaaat gaataacaaa aggcaccggt gcgctatctt cccctgccgc atgaaaccgg
      421 ttactgctga tttgccccat attgaccgtc atttgtttat caccggcttc aatccggcaa
      481 gtttccgcaa taatgacgcc ctgaaattgc atatttccgc cgggcagcgt ggtattccat
      541 ttatttccgg ccagagcaaa cattggcagc aacgacgcta acagaaataa tctcttcctt
      601 ttcatcgtta tttttatcgc acaagggtgg gcatccctgc ccgtaatgac gtccttgaac
      661 ctgggtaggt tattgatact gaaccttgaa ggttgcatcc gcattagctg cacccggggt
      721 tgcctcgccg attgcataat aacgcgcctg gaacggaatg gtgttggtac cgttattcag
      781 ggttgtttgt gcactaaatg tcgcaccgtc cagcgtcaga gcattgcctg ttctgtccag
      841 gatctgcacg ccaacgtttg ttgcactacc tgcagcagaa ctctgcagag ctagtacatc
      901 agtacgcgta gcatcaattg ccgtacctaa gaaggcaaca gcggcttttg tggcaacagt
      961 ggtatcgcaa tcattcagct gaatgttaaa accaacggca gagctggttg ctccagcctg
     1021 cttcaggcta gcggtacgaa cctgtcccaa ctgaacggtt tgatcaacag agcctgcatc
     1081 aactgcgcaa gcggcgttaa caacttcccc tttaaagtta atggtcccac catttaccgt
     1141 cgtagtatcg gccagagccg ctgcggaact gagggacaga gccgacagaa caacgattgc
     1201 cagagtttta attttcatgc tgctttcctt taaaaaaact atttctaaat cgacatgggc
     1261 agtcgttctg tacactttgt tttgtcaacg agtttgactg ccaacactgc acagttttcc
     1321 cccaaaagat gaaacatttg gggccatttt gactcataga ggaaagcatc gcgcacaaac
     1381 tttttcagtt tatttgttgg cttaatattc tattgttatc tttatttata gatttttata
     1441 ttgcatgagg tggtttttgg agagaagaat gaggaagatg cgtcgagcca cagaaacgtt
     1501 agctttacat atagcggagg tgatgtgaaa ttaatttaca agagaaataa tttacatatc
     1561 aaatagttag atgctttttg tcgtttttta atatttttat gcttgagaaa aaatacgtaa
     1621 cttatttatg atatggacag tttggcccca attgtcttgt gtttatttgt tttttaactt
     1681 tattatcaat aagttaaatc aaacctcttc tctttttaat ttttcgttta tgagattatt
     1741 tctttcccat aatccggcaa aacgagcagc attactggcg gtataacgca cagtatggcg
     1801 aatatttcga tgcccgagat aatcctgaat taaacgggta tctgcaccac gctccgccag
     1861 ttcataaccg caagcatgcc ttaacatatg aggatgagtc tgcgttacgg ttccagcttc
     1921 aataccggca tcgcgaataa tgcgataggc ctgctggcga gaaagccgac tcccgcggcg
     1981 agaaataaat atagcgtcgg tccggtcagc gcctttccag ttagcacgtt cctgggtcca
     2041 gcgttccacg gcttcacgct catcaaaacg taacgggtga acggtagaaa atccgttctt
     2101 cagtcggcga atatttattc gaccttcatt aaggtcaagg tcctgataat gcagatcaag
     2161 cagttcacta atacgcatcc catgccgata tgccaacaga ataagacaat aatctctggc
     2221 tcccgttgcc ccgtaacaaa ccgcctgcat catggcctga acttctttac cggtaagata
     2281 acgacgttta ctcacaatag tagtactcct aactgagata tatttgaatg aatacctata
     2341 ggaaacctca atcggtcaaa attagcccaa tagataaaaa gataaccaca cacgaagtta
     2401 tgtggctatc agtcaattac attaaacatc atacaaaaat aaaatatcac tgatgtgaca
     2461 acatctattt tcattgattt gattcaatat aacaatagat tacagaggat taacttccag
     2521 ttacttaaaa aataaagaaa aaagtgtgga aacaggagaa tttaagtgat gtgtaaactt
     2581 atgaatgatt ttgtttattt attttgaaga acataaacca ttagattttg tatgggtatg
     2641 gttaacttaa tgtttattat gataattaag aatcacactt acaataacat caattaataa
     2701 atggttaatc atttttgata tatcgtaaga ataatgtagt ttttaacacc atccctggta
     2761 tctcaactat ctctataaaa cagcatgacg ctgtcgtcct ctggctctat cccagatgcc
     2821 gtaaaaacgc cctgcattgc tggcggtata ccagacagta tgacgaatat tgcgatgccc
     2881 aagataatcc tagataagtc gcgtatctat tcctatattc gccaaagcaa aaccacacga
     2941 atagcgtaac atgtgcggat gaatctccag tgacaacccg gcattaccac cagaagtcga
     3001 gataatatgg taaaactgtt gccgagaaag cggattcccc ttacgtgata aaaataccca
     3061 ctcgctctca gcatgcggat aagaagtacg gatactcaac cagtttttta aagcctgaac
     3121 ttctttgttc aatagcgggt gcgttgttga aaagcctttt tttaatcgat ggatatatat
     3181 acactttgct ttaagatcaa tatccgaaat cctcaatcga caaatttcac tcgcccggaa
     3241 accatgaata aaacaaagca aaatcagaca ataattacgt gttgcatgag gccggtattt
     3301 gctgctttaa ggagtgactc gatttcacta tgggtaagga agttcctttt tttgttatca
     3361 gccttattcc tcatcgtttt cccttataat tacagacgcg cactagctgt gctgggttag
     3421 caataatcca acatttttat cctgattatg tttataaaag cgaacgtttg cttaataact
     3481 tacgtaagtg gaccagttct tctgagcgaa cttaaatgga gtagcgatag ttacttataa
     3541 taactttgcc atacaaacgc catgctcttg catgctatgt acctttatat atttatcaat
     3601 ctgatacgaa tctaataata accctcatca ttaatgcaac taatcttatc tatatatcat
     3661 gtgatatgtt ctgtaacaag taaaatcaac ataaaaaatt cagcaaaatt taatatttgt
     3721 tcaactagcc gtctcaaata tgacattatt agttaactgc atttgccaca aaatggctat
     3781 ttcatggaac gcagtaattt cttagtggct attatcatgc taattcttag atccctagcg
     3841 attattcctg cctggttcgt cagcttaggc catgagattg ttacattcct ttttatcctg
     3901 gagatatggg ggaacgcagg cgacgctttt tcatctacat ccgttggtaa atatagcttt
     3961 ggtcactacg acttaagcct tcctcaatca cagctattcg ccgggcgata cggcaaacaa
     4021 actaagtgaa acatcatcct gaaaacacca acatcaacaa gcctctccag atcgacatca
     4081 ggagtgacca gttacaagcc acaaaccaag aaacatattt catgatgaga attatgctca
     4141 gcctgatggc gagtaaaccc ttattttcac cactctcact gagttagctt gcaaatcttt
     4201 ctttgtttag attctaaata aattttttat aaaacatata gttaaaagaa aatctttagt
     4261 ttttgaagac caccgcaagt gttcgtctgg cttcatatgg catcttcttc ttagaaaaag
     4321 atcgacatat tttgtgacgc gaattgcaaa tctggttttg ttgtatggat tgcgtgattt
     4381 ttgatctggt ataacaggta taaagatgca ccacgatagt caatgagact gggcatctcg
     4441 taatctatgg caaacatcac ttcagttctt tcacatcggg tgatgaaaat gcacttcagt
     4501 ctgaaaggaa tatgaaaatg agatcaacag acactctatt ttatgactct gggtaaaatg
     4561 ggttgagtaa gtgatataac ttacgaatat tcaaatcaat taaaaatcag aagagatttt
     4621 atactcaggt atttaatctg gatctctgtt tatttaaata atgttaaaag agaattttca
     4681 caggagacct tatacaaaaa atataaaata cagttacccg ttgccaaaga cactataagc
     4741 ctggcaaaaa aatattacac aacataaatg ctaattgttt atgcgggctt tgtattgctt
     4801 tctgtatcct acaaatgagt gaaatttatg aaaaaggcta aaatactttc tggcgtatta
     4861 ttactgtgct tttcgtcccc attaatttct caggctgcga cactggacgt acgtggtgga
     4921 tatcgtagtg gaagccacgc ctatgagact cgactcaaag tcagtgaggg atggcaaaat
     4981 ggatggtggg caagcatgga aagtaatacc tggaatacca ttcatgataa taaaaaggaa
     5041 aatgccgcac tcaatgatgt tcaggttgaa gttaattacg caattaaact tgatgatcaa
     5101 tggacggtgc gcccgggaat gttaacgcat tttagcagca acggcacacg ctacggaccc
     5161 tacgtaaaac tgtcctggga cgcgacaaaa gatcttaatt ttggcattcg ctatcgttac
     5221 gactggaaag cttaccgaca acaagactta tccggtgata tgtctcgtga taacgttcat
     5281 cgttgggatg gatatgtcac ttaccatatt aatagtgatt tcaccttcgc atggcaaacg
     5341 acgctataca gcaaacagaa tgattatcgc tatgcaaacc acaagaaatg ggcgacggaa
     5401 aatgcctttg ttctacaata ccatatgacg cccgatatta cgccatacat agaatatgac
     5461 taccttgacc gccagggtgt ttacaacggc agagataatt tatcggaaaa cagttatcgc
     5521 attggtgtgt catttaaact gtagtagaca ggagacagtc acaatgaata aaactataat
     5581 ggcgcttgct atcatgatgg cttcatttgc cgcaaacgcg tctgtattac cggaaactcc
     5641 tgtgccattt aaaagtggta ccggagcaat tgataacgac actgtctaca ttggtttagg
     5701 tagcgcaggt acagcatggt acaagctaga tacacaggcc aaagataaaa aatggactgc
     5761 gttagctgca ttccctggtg gaccgagaga gcaagcaacc tctgcattta ttgatggcaa
     5821 tctgtatgtg tttggcggca ttggcaaaaa cagcgaggga ttgactcagg tatttaatga
     5881 cgtacacaaa tacaacccca aaaccaatcg ctgggttaaa ttgatgtcgc acgcgccgat
     5941 gggcatggcg ggtcatgtga cttttgtaca caacggcaag gcttatgtta ctggcggtgt
     6001 taaccagaat atcttcaatg gctattttga agatctcaac gaggctggaa aagattcaac
     6061 cgctatagat aaaatcaacg cccactattt tgacaaaaaa gaagaatatt atttcttcaa
     6121 taagtttctg ttgtcttttg atccctcaac acagcaatgg agttacgctg gcgaatcgcc
     6181 ctggtacgga acggctggtg cggcggttgt gaataaaggt gataaaacct ggcttattaa
     6241 tggcgaagcc aaaccaggat tgcgaacgga tgccgtattt gaacttgatt tcaccggtaa
     6301 taatttaaaa tggaataagc ttgatcccgt ctcatcacca gatggcgtcg ctggcggttt
     6361 tgcggggata agcaatgatt ctcttatatt tgccggaggg gccggattca aaggttcacg
     6421 agaaaattac cagaacggta agaactatgc gcatgaaggc ctgaaaaaat catatagcac
     6481 tgatattcat ctttggcata acgggaaatg ggataaatcg ggtgaattat cgcaaggtcg
     6541 ggcctacgga gtatcattgc cctggaataa tagtttatta attattggcg gtgaaactgc
     6601 aggcggcaaa gcggtgacgg attcagtttt aatttctgtg aaggataata aagtcacagt
     6661 acaaaactaa cgcttcaggg ccccggtaaa ggggccctgc tatcaatttg tcatttgtat
     6721 tacgcgaaaa ttatatgaac gcaataatat cgcccgatta ttactatgtt cttaccgttg
     6781 ctggtcagtc taatgccatg gcgtatggcg aaggactgcc attaccggac agggaagatg
     6841 cgcctcattc cagaattaaa caattagcga gatttgcgca tacgcatccc ggaggcccgc
     6901 catgtcactt taacgacatt attccactga ctcactgccc acacgatgtt caggatatgc
     6961 agggttatca ccatcctctg gcaacgaatc atcaaacaca gtacggcacc gttggccagg
     7021 cactgcatat tgcacggaaa ttactgccct tcattcctga taatgcaggg attcttatcg
     7081 ttccgtgttg ccgtggcgga tcggctttta ccgcgggcag cgaagggaca tattcagaac
     7141 ggcacggagc cagccatgat gcttgtcgtt ggggaacgga tactccgctg taccaggatt
     7201 tagtcagcag aacgcgagcc gcactggcaa aaaatccgca gaacaaattc ctcggcgtat
     7261 gctggatgca aggcgaattt gacttaatga ccagtgacta cgcgtcacac cctcaacact
     7321 ttaatcatat ggttgaagcc tttcgtaggg atctaaaaca ataccattct cagcttaata
     7381 atattactga cgcgccgtgg ttttgcggcg ataccacctg gtactggaaa gaaaatttcc
     7441 cgcattcgta tgaagctatt tatggcaatt atcaaaataa tgttttagcc aatattattt
     7501 tcgtcgactt ccagcaacaa ggtgaaagag gactgacgaa cgcgcctgat gaagacccgg
     7561 acgatttaag cacgggatat tacggttcag cgtaccggtc accggagaac tggacgacgg
     7621 cactgcgaag cagtcatttc agcacggcag cccgtcgggg gattatttct gacagatttg
     7681 tagaagcaat tttgcagttt tggcgcgaaa ggtgagcggg gagtttattc atgggggatg
     7741 tccttgttcg ttctcagagt agacaacaaa aaacaaatga gatcaaggta atatctcttc
     7801 acttgcgggc tcgaacagca ggcaattgcc aatataataa tctggcacaa gggtgaaaaa
     7861 cgcgcaccgc ataaaccgtt gttattgctt acagattagc cggatgcctt aatagacatc
     7921 cgcgcctttt cgattatggc tcggaaatct acgaacatct acacagttta ctggaacgtt
     7981 ttgggccaca gcgttcgcag caccgtcctg atatgccgtt ctggcgatta taagtcgacg
     8041 gattctggca aatagacgat gatgatatgc ttaatgatgt tcaaagaaat taggccatta
     8101 ttatattatt gcttgcattt tctttcattc ctaaaagcac ccgccattaa tcccaggcgc
     8161 at
//
LOCUS       DAJTYF010000150         8134 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTYF010000150 DAJTYF010000000
VERSION     DAJTYF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.150"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..883)
                     /locus_tag="OO590_003635"
     CDS             complement(<1..883)
                     /locus_tag="OO590_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6638847.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVD"
     gene            943..1221
                     /locus_tag="OO590_003636"
     CDS             943..1221
                     /locus_tag="OO590_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638848.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1222..1749)
                     /gene="gadE"
                     /locus_tag="OO590_003637"
     CDS             complement(1222..1749)
                     /gene="gadE"
                     /locus_tag="OO590_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6638849.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2548..3120)
                     /gene="hdeD"
                     /locus_tag="OO590_003638"
     CDS             complement(2548..3120)
                     /gene="hdeD"
                     /locus_tag="OO590_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6638850.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3375..3707
                     /gene="hdeA"
                     /locus_tag="OO590_003639"
     CDS             3375..3707
                     /gene="hdeA"
                     /locus_tag="OO590_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6638851.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3823..4149
                     /gene="hdeB"
                     /locus_tag="OO590_003640"
     CDS             3823..4149
                     /gene="hdeB"
                     /locus_tag="OO590_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6638852.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4213..4859
                     /locus_tag="OO590_003641"
                     /pseudo
     CDS             4213..4859
                     /locus_tag="OO590_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4901..5431)
                     /locus_tag="OO590_003642"
     CDS             complement(4901..5431)
                     /locus_tag="OO590_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6638853.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5587..6153)
                     /gene="slp"
                     /locus_tag="OO590_003643"
     CDS             complement(5587..6153)
                     /gene="slp"
                     /locus_tag="OO590_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6638854.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6400..7623)
                     /locus_tag="OO590_003644"
     CDS             complement(6400..7623)
                     /locus_tag="OO590_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638855.1"
                     /translation="MSIDFTLGIINTYHGDIYNCTTNTDNVKTPDTPKWPYDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2365 a         1572 c         1701 g         2496 t
ORIGIN      
        1 catccactaa tgccgtgaca tacatgccag gcagcaagtc accatttggg ttggggaaaa
       61 tcgcccgtaa cgtcacggag cccgtggttt catcaaccgt cgggtcggag aatttcagcg
      121 tgccggtctg gctgtaacgt ttaccatttt ccagattgag ctgtactggc gtactgccct
      181 gaacctgttt gatttgccca ctggcgacct cttctttcat gcgtaagaaa tcttgcaccg
      241 actgcgtgag atcgacataa atcgggtcca gacgttgtac ggtaaccagc gaatctgcct
      301 gattagcggt aacgagtgcg ccgacggtca ccgacgattt cccgctgacg cccgtaatcg
      361 gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc gctttggcga
      421 cggtgacatt ggcttctgct tcattcagct gggtgcgcgc ggtgtcgtaa tcctgacggc
      481 taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg gcattgctgg
      541 cggtagagag cgctttcgcc agcgagcctt tggcggagtt tagctcggcc tgtaaaggtg
      601 caggatcaat ctgatacagc gaatcgctct ggttcacttt atcgccttcg ataaagttgc
      661 gtttaatgat aataccgccc acctggggac gtatctcggc agcttcataa ggaacggttc
      721 taccgggcaa ttcgctcaac acattgaccg aactagggga gagtgtgacg acaccgacct
      781 caggcgtcat ggcggcggcg ttttccgccg atttgtcatc gcaggcggtg agcatcgcgc
      841 cgcagaataa caacggtatt aacagctttc ttcttctgtt cattttagtc cctgaaaatt
      901 cttgagaaat aacggttctt taaattgcgc gcgaggagag atatgaggga tttacagacc
      961 agcggcatag tggggttgtc agccagtaag gtgggataca ggcacagtga tcgacatggt
     1021 gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag cattcgggca
     1081 acgaagggtt ctactggtgg atacacatac caggggaatc aagtcgtttt agcctctcct
     1141 ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc cagccatcaa
     1201 tttcagttgc ttatgtcctg actaaaaata agatgtgata cccagggtga cgatgtcgct
     1261 catacgtctt agtttcagct tgtacataat gttgaatttc tgaatcttta atgtttgttg
     1321 cgaaatattg agcgtcgacg tgatattgct ttcattttta tttttcagaa tcagttgcat
     1381 aattttgaac tgattatgtg aaaaacataa ttgtgaattc ttatggggca agtgtttacc
     1441 ataagtaatc tcaagaagat ttttgatact ttctttgcgg ctaacaaaat aaataggcgc
     1501 tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct cggcatctaa
     1561 tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg tatcaatgat
     1621 aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga tttttatcat
     1681 ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt tcgtcatgag
     1741 aaaaatcata acttgctcct tagccgttat tgtttcgaac atgtcatcct tttcattatt
     1801 tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact ttgcccttcg
     1861 tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca cgcactccat
     1921 atattgatcc taagtaagat tcctggttgt tatcagcttg taaatagtaa acaaaaagtt
     1981 aacgtttgcg gattactgcc caagaataag ttatcaataa ccagtttgtg atctctgaag
     2041 aatattacta aagttaaaat gtaatccgat ttaaatatcg agtctccttg tttcgactta
     2101 agctggcaat tggattgcca gctttctttt aacgcattga aattacctga gtaaataacg
     2161 catttatgtc ttcaggtttt tatatagcgt ctggagacac ggatatttat gcaatgtaat
     2221 caacgccagt tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat agcatcaacg
     2281 cttacggaac tctttgttca ctttcccaca gcacttttaa aacattaaac ctgactacgg
     2341 aaaatatcag ccatgataat gtttgaaatg gctaatttgc catggagtga aaaaaattag
     2401 atgaaattca gtaggttgaa ataatcacta gcaggtaatt atttcaatga tagtgcgcaa
     2461 ttgatctaca acactgcgta gcggagagag tattaatcgg atcatagtca catcaagtga
     2521 ctatgatccg ggtgacaacc ggggtaatta ttgctgctta acgaacaaac tggcgaagct
     2581 gaacaggctg gcggcgctaa atatcagttc aattcccacc agtgtggaaa ccagcgttac
     2641 agacaccatc ggcgttgcac caaggaatat ccaggcaatg acgatatcca gcacaccaat
     2701 aacgagctgt agccagctgc ctttcattga acgctgacga taccaactca tcaggcgaat
     2761 aacccctgca acacagaaca aaccggcaat aaatgccgca atggcaaaaa tgcccagctc
     2821 cggtgcacgg atgaagaaat agccgatcaa taaataggcg actgcgacga ggaaaccgga
     2881 taataccggc cagaaattat gactgcggtt gctgaataac ccgacaataa gcgcaatacc
     2941 cgagcagatt aataatgcac ccactactgt gcttaaaata tcgccagaga cgaacgggaa
     3001 actgatacac agcaacccga cgataaacag cagcacggca ataaactgga ttgctctgcg
     3061 atgctttttc agcatctcca gatcaaactt caaaattgtt gccttatcta tatataacat
     3121 agaaccaccc tataaaatta agaagaaaat cccctgctat caatctatgc caaaaacgcg
     3181 tctaagaatg cagtcgattt aataaaaatt tcctaattgc agtatctgat gcatctgtaa
     3241 ctcattgtat tgaaataaaa atatctgatt ttgatatttt ccatcaacat gacatataca
     3301 gaaaaccagg ttataacctc agtgtcgaaa ttgattcgtg acggctcttt cactttatag
     3361 ttgaggatat tacgatgaaa aaagtattag gcgttattct tggtggtctg cttcttctgc
     3421 cagttgtgag caatgcagcg gatgcgcaaa aagcagctga taacaaaaaa ccggtcaact
     3481 catggacctg tgaagatttc ctggctgtgg acgaatcctt ccagccaact gcagttggtt
     3541 ttgctgaagc gctgaacaac aaagataaac cagaagatgc ggttttagat gttcagggta
     3601 ttgcaaccgt aaccccagct atcgttcagg cttgtactca ggataaacaa gccaacttta
     3661 aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc cgggaatgcg
     3721 ttacatcgta cttccttgca tattgaacag gccggaatat cttctttaaa agcagctatt
     3781 cctcctgttc atatataatc tctatattga atgggttaca aaatgaatat ttcatctctc
     3841 cgtaaagcgt ttatttttat gggcgctgta gcggctttgt cactggtgaa cgcacaatct
     3901 gcgctggcag ccaatgaatc cgctaaagat atgacctgcc aggaatttat tgatctgaat
     3961 ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg aagaaacagt atataaaggt
     4021 ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt gatcgaatac
     4081 tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa atcaagcatc taatgacttg
     4141 ccgaattaat gaggtacaag taaaaaggag tagcaagttg agccatcttg ctgctccttt
     4201 ttgcattttt atatgacagc agaattaatt atacgtctta tactcgcggc aattgcctgt
     4261 ggcgctattg gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg cacacatgta
     4321 ttaattggca tgggaagcgc cctgtttatg attgtttcga aatatggttt tgctgacgtg
     4381 ctgtctttag atcacgtcgg actcgacccc agccgtattg ctgctcaggt ggtgacgggc
     4441 gtcgggttta tcggtgcagg taacattctc gttcgtaacc aaaatattgt aggtctgacg
     4501 acggcagcgg atatctgggt gaccgccgca taggtatggt tattggcagc ggtatgtacg
     4561 aactgggtat ttatggttca gtgatgacct tgttggtgct ggaagtcttc catcaattaa
     4621 ccttccgcct gatgaacaaa aattaccatt tacagctgac attagtgaac ggtaatacgg
     4681 tatcaatgct cgactggttt aagcagcaaa aaataaaaac tgatttagtt tcgttgcagg
     4741 aaaatgaaga tcatgaagtg gtcgccattg atatccagct tcatgccaca acgttggtag
     4801 aagacctgtt gcgattatta aaaggtatgg caggggtgaa aggcgtttca attagctaat
     4861 ctgtgtggca gcgtagccag actcaccgta agcctgaaat tcacaccaga taatcaatat
     4921 gctgatggcg taccagctca ttgatccgtt taagattgag gatcatcatg atattgtgtt
     4981 tatggcagta gaacgttttt agcgatattt tcagtttctc cagaatatct tcagttgtca
     5041 tttgattcgc aatgcagctc agaacctgat gttgcttgcg cgaaagtgac acattcgctt
     5101 caggcacaga gcagtaactg ccattgatga gtgcgacaaa atcaatgata gtgatagtcc
     5161 gcggaacaaa ggtcaccggt actttcccca gacagcgttt aatattaaag ggggagaaaa
     5221 caatgacatg gacaggtttt aatcgttcaa tttgagtgag aaaattagaa tgaaatacat
     5281 tattcattaa tgtatcaata atgacgaagg cattctgatg caacattacg tcgatctctt
     5341 cttcgttctg tatatgaacg acattacctt tactcagaat gtttttcatc gcggtgaaga
     5401 acatcgtatc cctggtaatt ataagaaaca taaccgactc ctggtgcgga cattaacttt
     5461 ataatcagtt tgccaaaggt aattaacagg acagggctta taataataat agttagctta
     5521 ctgtatatta attttatgta atacatgatg cagatgctga gaaagcatct gcatctttcg
     5581 gtggtgttat ttgaccagct caggtgttac ctgactcacc gcattggtgt agtaaggcgc
     5641 acccaagccc ggttccggcc agaatgcacc atagccgtaa tcccacaggt tataggtggt
     5701 attaaccact tctctcaaat gccacacctg gatgccctgc atattcactt ccaggaagtt
     5761 atacgggact ttattgataa agcctggttg ttcaccctga atggtaccga ggatggtaac
     5821 aaagtgatta cgatagttca ctggatcaag gaagccgctt tgtctggcga gcagtcggcc
     5881 ctgatagttg gcttcaatat caggcttcgc atagctatcc aacggtaata cagcgatttc
     5941 taacaacgta tccgttttgc cattgataac gttgataacc ttcccaccaa agcgcgcttg
     6001 ttgaccaaca tataaccccg gctggttatg aacagcaaca aaactttttt gaatatcagg
     6061 ttggttattg cctttgatat tttgcggaat tgaactacat gcggcaagca aaaatgaaag
     6121 gctgaggatg agtgcacctt ttgtcatgtt catgttacta tccttatcaa caaatattaa
     6181 aaccataata gatatttctt tatagttttc atctgattct gaggtgatat gcctgatagt
     6241 aacgtaaacc tgtctgatgt ttatatccct tctgtttagt tcaattccgt tattttgggg
     6301 cgtggtatga gtatccatcg cccaataata taaccctttg ttttaaaatt acataaaatc
     6361 tatttttatt caatgattat aaagtgctaa taatcaatat tatggtatta tattctgata
     6421 atgatttgat aagcgaattc ttttgccaga tggtatttct atttccaccc cataatttat
     6481 atctttttca ttaggcagat ctctctttaa ataaaagggg tatgaaatct tttcgggttg
     6541 aagtgtctcg taatgaaatg ctatgaattc atttttagac attgttgccc atttttgaat
     6601 tttattgtat agagagggtg ttgtaaactc ttgaagttgt ttataacata tttttttgaa
     6661 ctcttgctta tattttaata tgtcatcaaa tgaaagattc actacctttg cctctaagaa
     6721 atcaattatt ctcgaagcaa tttccgcttt cgcgcgtaaa gagttgtcat taatagttaa
     6781 caatgtagaa aacaagccat tgatttgttt gtcaagatgt tgttcatatt tatgtagttt
     6841 ggggatggga ttttccttgt gtgaaaaaat agtgttaaat aaatttgcgt cagtgaaatt
     6901 tatattaagt agatcttcaa aataaaattt tgataaggtt aggactagct tggacatatc
     6961 ttgccctgtc atgttgaaat caataaagtc gcatccagca agggcttcca tcctattttc
     7021 atttttactg tgctcaatat acagcttcgc catttttaag tacaagcttt gaatgtctct
     7081 tatatcggtt ggtaagtcag ctcgttgccc agcaaaattt ttattatcgg aagaaacggt
     7141 ttggacactg aatctttcgg gtattccttc ttctacgaga attttagtta tgattggatt
     7201 gttgggttct ggattaacga caacaatgat ttgacataca gaatccttat ccttttcgta
     7261 tctaatgtta tgaatctgat taagtattga ttgtgtctgt tgcgtaagtg gtgtcgaact
     7321 tatcgtttca atgttattaa taagacttac aagttgaacc acaacttttg agtagtctgc
     7381 atttgtatat gtggtgttcg tgtggcatgc attaatttgt tggagttgtt gttgcgctaa
     7441 atcaaattgt tcagggttgt atccttctcc agagaatgtg gaattaatgg gttgttgttc
     7501 ttcccaatta tcataaggcc acttcggtgt gtctggcgtt ttaacattat cggtatttgt
     7561 tgtgcagtta taaatatcgc cgtgatatgt atttattata cctagggtaa agtcaattga
     7621 cataacattc cttattcatc atctgtttta tgctaatgtt tttaaattgg ataattagaa
     7681 caggaatgga ttttcttcta cacacaataa atataatgcg ttatatcttc ctgatataat
     7741 tacctttaaa ttccctttca tttccacccg gtaatatttg tgtataaaaa aagggcagtg
     7801 atagtatcgt aaaggaaaac gttaacaact atcaaaaagc gctcatttta aaaagttatt
     7861 atgttctccg atagtgtgtg aagtaatatt tttgtgatgt tttagatgtg taataatgaa
     7921 ccaggttaaa gtgtatttaa aaatgtgttg tattagcgac tgaaatctgc cttttaaaaa
     7981 ggaggagggt actgagacaa agagtcattg cttctccgat aaaatgtcag agaagcatga
     8041 ttacgtggtt attgtctgtt gatgacttgt tgaccgagcc gtcatcattc tcaatgcatt
     8101 agttaaatca tttgtgattt ttaaggatta tcct
//
LOCUS       DAJTYF010000151         8068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTYF010000151 DAJTYF010000000
VERSION     DAJTYF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.151"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..301)
                     /locus_tag="OO590_003645"
     CDS             complement(<1..301)
                     /locus_tag="OO590_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6638856.1"
                     /translation="MGLPPLSKTPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAV"
     gene            complement(301..1008)
                     /locus_tag="OO590_003646"
     CDS             complement(301..1008)
                     /locus_tag="OO590_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6638857.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1023..1700)
                     /gene="ydjY"
                     /locus_tag="OO590_003647"
     CDS             complement(1023..1700)
                     /gene="ydjY"
                     /locus_tag="OO590_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6638858.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(1705..2415)
                     /locus_tag="OO590_003648"
     CDS             complement(1705..2415)
                     /locus_tag="OO590_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6638859.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(2582..3388)
                     /gene="xthA"
                     /locus_tag="OO590_003649"
     CDS             complement(2582..3388)
                     /gene="xthA"
                     /locus_tag="OO590_003649"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6638860.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            3834..4412
                     /locus_tag="OO590_003650"
     CDS             3834..4412
                     /locus_tag="OO590_003650"
                     /inference="COORDINATES: protein motif:HMM:NF012428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6638861.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGSIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDATCAVIVEPI"
     gene            4428..5054
                     /locus_tag="OO590_003651"
     CDS             4428..5054
                     /locus_tag="OO590_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6638862.1"
                     /translation="MPASNAFLQGLRELCDRHNALLIFDEVQTGVGRTGELYAYMHYG
                     VTPDLLTTAKALGGGFPVGALLATEECARVMTVGTHGTTYGGNPLASAVAGKVLELIN
                     TPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRGLGLLIGCVLNADYAGQAKQISLEA
                     AKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDRFAAACEHFVSRGSS"
     gene            5051..6784
                     /gene="astA"
                     /locus_tag="OO590_003652"
     CDS             5051..6784
                     /gene="astA"
                     /locus_tag="OO590_003652"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6638863.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAAGWLAAGRETGQALSALEDLDGLLFTGSANTGYQLHRQLSGQPEKILALEMGGNNP
                     LIIDEVADIDAAVHLTIQSAFVTAGQRCTCARRLLLKSGAQGDAFLARLVAVSQRLTP
                     GNWDDEPQPFIGGLISEQAAQQVVTAWQQLEAMGGRTLLAPRLLQAGTSLLTPGIIEM
                     TGVGGVPDEEVFGPLLRVWRYDTFDEAIRMANNTRFGLFCGLVSPEREKFDQLLLEAR
                     AGIVNWNKPLTGAASTAPFGGIGASGNHRPSAWYAADYCAWPMASLESDSLTLPATLN
                     PGLDFSDEVVR"
     gene            6781..>8068
                     /gene="astB"
                     /locus_tag="OO590_003653"
     CDS             6781..>8068
                     /gene="astB"
                     /locus_tag="OO590_003653"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR6638864.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQ"
BASE COUNT         1896 a         2052 c         2216 g         1904 t
ORIGIN      
        1 ccactgccag cagtttatcg gtgctgccgg tacgctcggc gactttcata ctggtgatat
       61 ccacgcaaaa ctcacacagg cacatttgcg caatgcgcgc gctgacaagc gatcgcacta
      121 ccggatcgag cggtgagcgt ttgcgctcca gccacccaac aaacattgac accaaataaa
      181 agataaacgg gatccgcccc caccagcgaa tcgggcttag cacctcgccg taatggcgac
      241 gatgcagcca cgcctgcgga cgtaaaatga aaggagtttt gctaagcggc ggtaaaccca
      301 tcagtcattc ctcttctggc gctcaagcca tattttcttc gccataaaaa tcaccacggt
      361 cagggcaaac agaataaaca gttcggtgac aaaccagaaa gtaccgcctg ttaacatgct
      421 gcccgcccag gaataaacaa tagtcgccgg taattgacca agcccggtgg cgataaaaaa
      481 cgagcgaaaa cgtattgaag tcagaccggc agcatagctg attggatcga aagggacaaa
      541 aggcaataac cgacagacca ggatggtgtg tttgccgtag cgagtgaaaa agccgtccat
      601 actgtcaagc acggttttgc cggttaattt ttccaccact tcgcggccca tcactctggc
      661 gataaaaaag cacagcgccg cgccggccat cgaactggtc cacgacagca agccgcccca
      721 gaacgcgcca aacagcgacg cattggcaaa ggtgatcaaa aacgcaggta gcggtgcagc
      781 aatggcctgt aaaatcatca acaagaacga gacaactgcc gccagtgcgc cgtaagactg
      841 aataaagcgt tctataccct gttggtccac ggcggcaaac gccgcaacgc tgcgattgat
      901 aaactcatgg acgccgggaa gcagcgccca tgcgagcagc atagcgaaca acaggatgat
      961 gagggttata cggtagtacc agattttacg cgattgtatc ttcatcatcc tttatcctgg
     1021 ctttattcgg tgattttaaa ggtaaccgtt gccaacgtgt tatccgccgg gagaactgag
     1081 gcattgcctt tgaatttcac ttcgccgcgt ttttccaccg caccataagt gtatgttgca
     1141 ttgctgacga tgccgaccgg gcagctatcc agacacacca ggcaacccgt tttcttctct
     1201 tctgctgccg ttaaattacc gccaaagcgc atgtccagtt tcttgccatt actgtcaaca
     1261 atcacttcat cgaaggaata cgcttttgcc gccccttgcc agttgaccga aatatccagt
     1321 ttgctgcctg tgacatgagt cgtttcttta ttatccatcg tcatgttttc gcccggcgta
     1381 ccacctgcct ctttcagggc ttcataaaat gctttcgggg tcgcataagc cataaacagc
     1441 gatttatgtc cgttggagcc atctttaaac acaataccgt gacgagtgtc gtcggtgagg
     1501 aaacgaccat taatttgcac cagcatggtt accgtatggt tattggcatc gacgcgcagc
     1561 ggttggctgt tcgaaagtcc gtcgtattcc acttttgccg ccgcattctc tttttgatcg
     1621 cagccgctaa gcccggcaac agccagtaag cagagtgcag ccagtccttt tttccatgac
     1681 actgaataat gttgcgacat cgttctatcc ttcatttttt gggtgagtaa gtgggctgcg
     1741 gcgcgaagca gacagatcca catgtttgtg tcgggcgtag agttttgcga gctggatgag
     1801 aataaacagc gccaggcccg ccagacagag ttgtaaaata aagcgcagcg taatgccttc
     1861 attggcgaga tcgcttgcca tcacggtata aataacaata ccgggcaggg tcgtgagtgc
     1921 cgaaataagg gtataaggcc agaaggcgat ggtggttaat ccgtaagcgt aattttgaat
     1981 attgtaagga aacaacggga ttaagcgggt cagaataaga aaatcaatac cgttacgcgc
     2041 aatgcctttt tcaatggcct ggaaggtatg gctatggccc acgtatttca gcagtaaatc
     2101 acgtcccagc cagcgcgcca gcaggaatga gcacgaggag gccagcgttg cggcaattaa
     2161 tgagagtagt gtccctaaga gcgggccaaa aactattcct ccagcgatca ccagtatgct
     2221 tcctggtaac agaaagaggg gggcattgat gaataacaga atatagaggt tataaccgaa
     2281 caatccgctc tggcgaatga gtgtctgtaa gtggggtaaa tcggtgagca gatcgaataa
     2341 accgaaagcg tggatagcgt aaatgaccag cgcaaaaata agacaggcaa acagaaagtt
     2401 acgctcagcg ttcatcgtgg agtttcctat atcaaacttg ataatagaga agtggtcaga
     2461 caactaaaaa cgcagtgacg gtataacaaa ggacggcagg caacaaatca ttactgacag
     2521 tttgttgatt tttctcaagg ttaattctcc tgacccagtt tgagccagga gagctgctaa
     2581 attagcggcg gaaggtcgcc cagacggggg cgtgatcgga cggtttttcc atgctgcgaa
     2641 tttcatagtc gatgccggtt tctacgcaac attctgccag cggttggctg gcgagcagca
     2701 ggtcgatgcg cagaccacgg ttatcgtcaa aaccttttga gcggtaatca aaccatgaga
     2761 aacgatctgc tgtttgcgga ttagcatggc ggaaggtatc gaccaccccc cagctcatca
     2821 gcctgtccat ccattcgcgc tcttccggca ggaaagagca tttaccggta cgcagccagc
     2881 gcttacggtt ttcttcgcca atgccgatat ccagatctgt agggctgata ttcatatcgc
     2941 ccataatcag taccggatta tcacgtttga gttcggtttc caggtagttt tgcagattct
     3001 gataaaactg cgcttttgcc gggaatttta tcggatggtc gcggctttca ccctgcggga
     3061 agtaaccgtt gatcacggtg acataaccca gcagtgaggg gatttccgcc ataataatcc
     3121 gccgctgcgc ctcttcgtcg tcaccgggaa agccgcgacg cacggcaatc ggcgtctctt
     3181 tggtcagcag cgccacgcca taatggcctt tctgcccgtg ataaaacacg ttgtagccga
     3241 gcttcgccac ctcttcgagc ggaaacatat cgtcatgaac ttttgtctcc tgcaggccaa
     3301 tcacatccgg ttggtgcttt tcgacgatgg cttcaagctg gtgaggtctg gcgcgcaggc
     3361 cgttgatatt aaaagagaca aatttcatag tcgctgccat ttattcagat aaagagtgcg
     3421 tggatggtag cagaatttcg cgttgcttac cgcctgttgt taagtgattt gatcgatggt
     3481 tgttaatgat gtcaacgatg gcgcaaaaaa tgcccgcttt tggtgcgcgc tgcgtcagaa
     3541 tggcgcagta atttccagta aatttcgtca atgatcacaa ttccagaatt atattttact
     3601 tctgcataac attgcgtttt ttattctttt tattcaccca catcgcaaac gtattcactt
     3661 tatatgcact ttaaatgcat atggtttggt tataactcct tgatttccag ttagcctccg
     3721 ccgtttatgc acttttatca ctggctgaca cgaaccctgc aatctacatt tacagcgcaa
     3781 acattactta ttgttaacat acaaataacg aattatttac tgtagaggtc gctatgtctc
     3841 agccaattac gcgtgaaaac tttgatgaat ggatgatacc tgtttacgct ccggcaccct
     3901 ttataccggt acgtggcgaa ggttcgcgct tgtgggatca gcaggggaaa gagtatatcg
     3961 acttcgcggg tagcattgcg gtgaacgcgc tgggccatgc gcatccggaa ctgcgtgaag
     4021 cgctgaacga acaggcgagt aagttctggc ataccggcaa cggttacacc aacgagccgg
     4081 tactgcgact ggcgaaaaaa ttgatcgacg ccacgtttgc cgatcgcgtc ttcttttgta
     4141 actccggtgc ggaagccaac gaagcggcgc taaaactggc gcgtaaattc gctcacgacc
     4201 gctacggcag ccataagagt ggcatcgtgg cgttcaaaaa tgcgtttcat ggtcgcacgc
     4261 tgtttactgt cagtgcgggt gggcagccag cctattcaca ggattttgcg ccactgccgg
     4321 cggatattcg tcatgctgca tataacgata ttaactctgc cagcgcgctg attgacgacg
     4381 ccacctgtgc ggtgattgtc gagcccatct agggcgaagg cggtgtggtg ccagccagca
     4441 acgcgttttt acaaggtctg cgtgaattgt gtgaccgcca caatgccttg ctgatttttg
     4501 atgaagtaca aaccggcgtc gggcgcactg gtgagctgta tgcctatatg cactacggcg
     4561 tgacgcccga tctgttaacc accgccaaag cgctgggcgg cggtttcccg gtcggtgcgt
     4621 tgttggcaac cgaagagtgc gcccgcgtga tgaccgttgg cactcatggc accacctatg
     4681 gcggtaatcc gctggcctcg gcggtagcag gcaaagtgct ggagctcatc aacacaccag
     4741 agatgcttaa tggcgttaaa cagcgtcacg actggtttgt tgagcgtctt aatactatta
     4801 atcaccgcta tggtttgttc agtgaagttc gcggcttagg tttgttgatt ggctgtgtac
     4861 tgaatgccga ttacgccggg caagcgaaac agatctctct ggaagcggcg aaagcaggcg
     4921 tgatggtact gattgcgggt ggcaacgtgg tgcgttttgc gcctgcgctc aatgtcagcg
     4981 aagaagaggt gacgaccgga ctggatcgct ttgcagctgc ttgcgaacac tttgttagcc
     5041 gaggttcatc atgatggtca tccgtcccgt tgagcgctca gatgtctcgg cgctgatgca
     5101 gcttgccagc aaaacgggcg gcggcctgac gtcgcttccc gccaatgaag ccacgctttc
     5161 ggcgcgtatc gaaagggcaa tcaaaacctg gcaaggcgaa ctgcctaaaa gcgagcaggg
     5221 ctatgtgttc gtgctggaag atagcgagac aggcaccgtg gcggggattt gcgctattga
     5281 ggtggcggtt gggctgaacg atccctggta caactatcgc gtcggcacgt tggttcacgc
     5341 ctcaaaagag ctgaatgtct ataacgcatt gccgacgctg tttcttagta acgatcacac
     5401 cggcagcagc gagctgtgca cgctgtttct cgacccggac tggcgcaaag agggcaacgg
     5461 ctatttgctg tcgaaatcgc gctttatgtt tatggcggct tttcgcgaca agtttaatga
     5521 caaagtggtt gctgaaatgc gcggggtgat tgacgaacac ggctattcac cgttctggca
     5581 aagcctcggt aaacgcttct tttcgatgga ttttagccgc gccgattttc tctgcggcac
     5641 cgggcaaaag gcatttattg cagaactgat gccgaaacat ccgatctata cccacttttt
     5701 atcccaggaa gcccaggacg tcatcggtca ggtacatccg caaaccgcgc ctgcccgcgc
     5761 ggtgctggag aaagaaggtt ttcgttaccg caactatatc gacatcttcg acggcgggcc
     5821 aacgcttgag tgtgacatcg accgcgtgcg cgccatccgt aaaagtcggc tggtggaagt
     5881 ggcagcaggc tggcttgccg ccgggcgtga aacgggtcag gcgctgagtg cgctggagga
     5941 tctcgacggt ttgctgttta ccggtagcgc caataccggc taccagctgc atcgccagct
     6001 ctccggtcag ccggagaaaa ttctcgccct tgagatgggc ggtaataatc cgctaattat
     6061 cgatgaggtg gcggatatcg acgcggctgt ccatctgacc attcagtcgg cgtttgtcac
     6121 agccgggcaa cgctgcacct gcgcccgccg tttattgctc aaaagcggag cgcagggcga
     6181 tgcgtttctt gctcgtctgg ttgccgtcag ccagcgatta acgccgggca actgggatga
     6241 cgaaccgcag ccgtttattg gcgggctgat ttctgaacag gccgcacagc aggtggttac
     6301 tgcctggcag caactggaag cgatgggcgg acgaaccctg cttgcgccgc gcttattaca
     6361 agcagggaca tcgttgctga caccagggat cattgaaatg acgggcgttg gcggtgtgcc
     6421 ggatgaagag gtgttcgggc cgcttttgcg cgtctggcgt tatgatactt ttgatgaggc
     6481 gattcggatg gcgaacaaca ctcgcttcgg actcttttgc ggtctggttt cccccgagcg
     6541 ggaaaaattc gatcaactgt tgctggaggc gcgggcgggg attgttaact ggaacaaacc
     6601 gcttactggt gctgccagta ccgcgccatt cggcggcatt ggtgcttccg gtaaccatcg
     6661 ccccagcgcc tggtatgccg cagattactg cgcatggcct atggcgagcc tggagtcgga
     6721 ctcgttaaca ttgcccgcca cgcttaaccc cgggctggat ttttccgatg aggtggtgcg
     6781 atgaacgcct gggaagttaa tttcgacggg ctggtagggc tgacgcatca ttacgcgggc
     6841 ctgtcgtttg gtaatgaagc ctctacccgt caccgttttc aggtgtctaa cccgcgactg
     6901 gcggcaaaac agggcttact gaaaatgaaa gcccttgccg atgcgggatt cccccaggcc
     6961 gtgatcccgc cgcacgagcg tccgtttatt ccggtgctgc gtcagttggg attcagtggt
     7021 agcgatgagc aggtactgga aaaagttgca cgccaggcac cgcactggct ttccagcgtc
     7081 agttccgctt cgccaatgtg ggtagccaat gcggcaacga tcgcgccatc tgccgatacg
     7141 ctggatggca aagtgcatct caccgttgcc aacctgaaca ataaatttca ccgttcgctg
     7201 gaagcgcccg tcactgaatc gctgttaaaa gcgattttta aggacgaaga gaaatttagc
     7261 gtccattcgg cgttgccgca ggtagcgttg ctcggtgatg agggggcggc aaaccacaat
     7321 cgtctcggcg gtcattacgg tgaaccgggt atgctacttt ttgtctacgg gcgagaagaa
     7381 ggcaatgata cccggccttc ccgttatccg gcgcgacaga ctcgcgaagc cagcgaggcg
     7441 gtggcaaggc ttaatcaggt gaatccccaa caggtgattt tcgcccagca aaacccggac
     7501 gttatcgacc agagcgtttt tcataatgac gtgattgccg tgagtaaccg ccaggtgctg
     7561 ttttgccacc aacaggcgtt cgctcgccag tcacagttac tggcaaacct gcgggcgcgg
     7621 gtgaacggtt ttatggcgat agaagttccg gcaactcagg tttccgtgtc ggatgcggtg
     7681 tctacctatc tgtttaacag ccaactgttg agccgcgatg atggttccat gatgttggtg
     7741 ctgcctcagg agtgtcggga acacgccgga gtatggggtt atctcaatga actccttgcc
     7801 gctgacaacc cgattagcga actaaaagtc tttgacttac gtgaaagcat ggcgaatggc
     7861 ggcggcccgg cgtgcctgcg gttgcgggtg gtattgacag aagaagaacg ccgggcggtg
     7921 aatccggcgg tgatgatgaa cgataccttg tttaatgcgc tcaatgactg ggtggatcgt
     7981 tactaccgcg atcgtcttac tgctgccgat ctggccgacc cgcaattgct gcgcgaaggg
     8041 cgggaagcac tggatgtatt gagccaat
//
LOCUS       DAJTYF010000152         8059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTYF010000152 DAJTYF010000000
VERSION     DAJTYF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.152"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..1232)
                     /locus_tag="OO590_003654"
     CDS             complement(<1..1232)
                     /locus_tag="OO590_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6638865.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRNAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQR"
     gene            complement(1229..2107)
                     /locus_tag="OO590_003655"
     CDS             complement(1229..2107)
                     /locus_tag="OO590_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6638866.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2118..3025)
                     /locus_tag="OO590_003656"
                     /pseudo
     CDS             complement(2118..3025)
                     /locus_tag="OO590_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3012..3245)
                     /locus_tag="OO590_003657"
     CDS             complement(3012..3245)
                     /locus_tag="OO590_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638867.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3242..4099)
                     /locus_tag="OO590_003658"
     CDS             complement(3242..4099)
                     /locus_tag="OO590_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6638868.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4124..4453
                     /locus_tag="OO590_003659"
     CDS             4124..4453
                     /locus_tag="OO590_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6638869.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4443..4751
                     /locus_tag="OO590_003660"
     CDS             4443..4751
                     /locus_tag="OO590_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6638870.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4793..4969)
                     /locus_tag="OO590_003661"
     CDS             complement(4793..4969)
                     /locus_tag="OO590_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR6638871.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4966..5376)
                     /locus_tag="OO590_003662"
     CDS             complement(4966..5376)
                     /locus_tag="OO590_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR6638872.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5373..5651)
                     /locus_tag="OO590_003663"
     CDS             complement(5373..5651)
                     /locus_tag="OO590_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638873.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5726..>8059)
                     /locus_tag="OO590_003664"
     CDS             complement(5726..>8059)
                     /locus_tag="OO590_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR6638874.1"
                     /translation="EPCLFGWQALDAGVRAVVLCEGEIDCMSYAQYGISALSVPFGGG
                     KGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASRLGEHRCRLVTLPYKDIN
                     ECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWESLAD
                     KFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPSTVT
                     GIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSETKR
                     HKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAELPD
                     YNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIGMLC
                     GLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRTRVKTGVTYTYKDDKRHG
                     MDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIGNAECLCIMVDHP
                     RHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQATCCKMP
                     PVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMKCGIGD
                     DDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITDLTDNL
                     FIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVPLFLDE
                     QSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         1914 a         2194 c         1848 g         2103 t
ORIGIN      
        1 ttacgttgtt tcgtgatatt gatttctgcg atgcccctgg cctgcgtatc cgggttgtac
       61 acttcatccc tgtaaagcat cagaatgatg tctgcatccg cctctatttc tccggaattt
      121 ttcaggtctg agttcatggg acgtttattg ggtctggact ccacaccgcg ggagagctgg
      181 ctcagcgcaa tcaacggaaa accaccggat tttgccaggc ctttaagccc ctttgagatt
      241 tcacccacgg caaggtcatg acgccccgtg gttcgggttt ttatcagccc gagataatca
      301 accaccacca gcgccgtttc cggatattta atcaggtggt gtttcgttgt tgcgcatatc
      361 tcatcaatgg ccaggttcgc ctggtccacc atccagatat tgcgcccggt catccgcccc
      421 accccttgtg agaaacgcgt ccagtcttca tcttcaaagt gagccacaga tttcaggcgt
      481 gatactggca tccctccagc cgcagacacc atacgttcac caatctggat gttcgccatc
      541 tccatggtga acagaagcac accatgcccc tgctcagtca ccttgtcgat gatgtccagc
      601 gcaagttcgg ttttccccat cgaaggacgg gcggcaatga ataccaggtc tccgggctcc
      661 ataccgcctg tttttgcgtc cagttcatca ataccggtca tcaacgtcct ggatttctcc
      721 agcccctgat tccggcattc aacacgctca accacttccg gaagcacatc atcaatatgt
      781 accggctgaa tgacgccctt tcctgtcgac agtgtgacca tcatgttctg cgcatccttc
      841 agggcatctt cagctgcttc acaggtatgc gcatcacgta atttctgcag cgcctcattc
      901 agtgtttttt ctgcattgcg cagtgcggca ttgcgccgca acgctgcaac atagtgctcc
      961 agtgaagact tcacccaggt tttacgcccg gtatcagtaa tcaccggggc aagttccggc
     1021 atctcattac acaacagcac ggggtcaatc actcctgaaa cacgggcctg tctgcagatg
     1081 cctgtataga tatcctgata cgctcgtaca gaaaaaacgt ccgctggcag tgtggccaga
     1141 atatccatca cttcaggatc tgctccgcgc agaaagaacg cgccgatgac agcgccttca
     1201 aggtcatcgt tacgccatac tggagttgtc atgcagccac acctctgata cgagaacggt
     1261 aactgggcca gttaaacgac aaccagttgc gtcccccgtc tgtgatcctg tcggcaattc
     1321 ggggactgat gaacgcccac aactcttccg gtgagaggtt actgatcaga atggtgggca
     1381 agatactttc gtacctggcg ttgataattt cctgcaaaat agccatttca gccgcgctgc
     1441 caaactgaac gccaacttcg tcgatgatca gcaaatccat tgacgcataa cgctcaataa
     1501 cttcatccgc tgttttttcg ctgtcattcc gccagcaatt tttcacagca cgggtaaggc
     1561 gcatcacgtc ggtgatctcc acactggcca gatagttacg gatgatgtgt tttgccatag
     1621 ccacagccag atgatttttt ccggtaccac aactgccggt cataacaaga ctggtaccgt
     1681 tctccagcat atctggccaa ttctccgcat agcggcgaca ggccgcaaga tttctggctg
     1741 cgccaggatt aacctccaga taattatcaa actcgcagtc ccgaaaacgc agggcaattc
     1801 cggcgttatc agtcagctct tccgccttga tggacgacag ctccatggtc aaatcgttgg
     1861 cctcagcgat taagcagtca gggcagcatg aaattttttc tctgtcctcg ccattacgat
     1921 cgctccacac cagtatatgc gtgtgatatt cgccatgttt ttcgcaatat ccgcgacctt
     1981 cacgcatcag gcaggaacga taaggccatg gcttttcgcc cttctgagca aatgcaatct
     2041 ctgcccgtaa ctcatccatt cgcgcctgta gtcttttttg ttgttcacgc aggttaaacg
     2101 tcatcatcgc tgtcacctca gaatgtcagt ttgtcactgg atttaccgaa tttgtcagac
     2161 atggctccca ggccagccag gacatcgacc tgtcgctgtc gcccacctcc gggagcggct
     2221 ggctgttgcc agaagtcttc gaagtgacga tcgggtccaa agaacgtcgc agcctgcttc
     2281 acgaactgtg tgccggtatt tcctgtggca cgtacccagg cagcatagcg tttcacgcca
     2341 tcaagcatgg cttcaggttt tattccctcc ctgagccggg ctttccaggc tttgaaggct
     2401 gcggattttg agttaccacc agcacgtttt ggatattcct gccaggcctg ttcaaattcc
     2461 ggtgaatatt cctgtcggga agaacgcgct ggtgcagacg cgtcagcaga tgcgccaata
     2521 gtgtttttac tctctgtagt attctctgaa gtaatctctg ttgtattctc tgtaagatcg
     2581 aaattggttt tcccttctcc gcggcgaggg gtttcccgtg tccgcggtga aggctttccc
     2641 tcctccgcga aattgggttt tacagtttcc cgaaaacggg tttccccatt tcgggaaact
     2701 gattgttttc attgataatt tcattaaggc gctcacaatc tatacggtag aacattttgt
     2761 gctcaagacg cttgttggtt tcaaccaaaa tgcctctgga cacaagatgc ttacgcgcta
     2821 cagcctgttg ttcaaatgta agtccggttt cgtgttgtat ctcttcacgc gttttatgta
     2881 cgccttccgc tgcatgtgct ttatcctacc agtaaaaaat ctgaccaaag aaaataacag
     2941 cgtgcggact tcccatgtat ttaacgagcc cagggtaata agcaaccgga tgtccaaaat
     3001 cgagcagaag atcagacgga cgcatagcca cctcccaggc gtttaaacat ttttccgggc
     3061 tgaaacgcca ccagcggata actcagggta tgagtacgtc cctgaacctg gcagacaacc
     3121 ttctggcttt cggtactgac caggcaaacc cgcagaacgt agccgttgct ggtggtgaac
     3181 cactgcccca cacgggggca atggttgtat cggtgataca ggaaattaac gatgtggcgg
     3241 atcatgggag cacctccttg tcagaaccat tcagcctgga atcaacaagt gcagcaccaa
     3301 aaacagcatc acctacacgg tcgtacagtt tgccagccag cggagattca acggccttaa
     3361 gcattgggta aagctggctt gtccagattt gatggatttc acgcaaatgc aggtatacgc
     3421 ctctggcgtt ttgtgcgaca gatgacatgt cagccgcacc agctcctgat aagctcctct
     3481 ccatctggtt aaaggcattg atgtatgcct ctttgaaccg ggcggcacgt ttaccagtga
     3541 agcccatagc aaggaaggcg aagccgtcgc gggtaatttg gtagcaggga agtttgcgag
     3601 taccgccgtt gggctggcgt accaaaattg atgtctccgc aaaattgcgg gcacaaaact
     3661 ctggagaaca atccaaaatg cggatctttt tcagaacatc gtcatgacgt ttagagaaga
     3721 agttggcaac agccagggat gaagtaacag cctgaccatc aacgatggca atttcaggtt
     3781 gagagagggt tgggagagta gtcatggtga cagcccctat gttgaattca aagaactcac
     3841 cacatgggac gccaatcaca gaggtggtga gacgtacagg gttggcgtaa ccggtcaaca
     3901 tagaacccgg cgcatcttgc gatgcccctg cacgccccac cataatttgg gcgtagcaat
     3961 gctcatgaca cgaaaaaacc gcatgagcgc ggttatgctc tatattgaat ttcaggacgc
     4021 caatcccggc acccgcttta taaggtgcag agacagtgta acgtcccgaa attgcagaat
     4081 caatatattg tgcagatata acgattttgt tatgttttgc tgtatgaact acactatcga
     4141 atactacagt gaagaggtcc gactggaggt cgatcagctt ccattgagta tgcgtgccag
     4201 ataccagcat cttgttgaac gcatgaaggt atacggcagc aatctcggag aacctcacac
     4261 cagcgccttt ggtgacgggc ttttcgaact cagaattaaa ggcaacgatg ggatcgcgcg
     4321 tgttttttac tgcactctga cagggaaacg catcatcatg ctgcatagtt ttgtaaagaa
     4381 aacgcagaaa acaccgccag ccgaacgcaa gaaagctgaa accagaatga aggaggttaa
     4441 gcatgactgg taaacgcact ctccccacca tgacacacga cgaaatggca gccaaatgga
     4501 tggaagaccc ggcttttaaa gcagaatacg acgctatcgc tgacgaattc gcactgcttg
     4561 atgaaatgct ggcagcacgc aaagaagctg gcttaactca ggctgaagtt gccgagcgaa
     4621 tgggaacaaa agcgaccgcg atcaccagaa tggaaagtaa tctcgcatca ggtatcagcg
     4681 gcccatcatt tgccacacta aaaaaattcg cccgcgctac tggaaaaaaa ctccagatcc
     4741 gcttcgttta accacaccgc gccgtcattc tggcggcgca ccggataaat agtcatttcc
     4801 tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtagttg aaggttttta
     4861 cgtcagattc ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt ggaaggtatt
     4921 tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg tcgcctcatt catacctcct
     4981 gtcggtaaat ctgacaccct gaccaatagc ccaggctgtt gtgtactcga tcagacttgc
     5041 catgcgcttc acgctcatct gcgcgctgct ttcgcgaatg ttgacgcatt ctccttcaag
     5101 gccgggcaaa acatcagctt cctgttttgt tgccactgca tgaccgctga tcaacaaaac
     5161 cttccattgt tctggtttta accatttgcc gcaccattga acctgacgag cgatatccgc
     5221 cagcatcgcg tgaaattttg cgttctggtc aagatttcgc ttgtagtcag taatgcggat
     5281 ggtgactggc ttgtctttat cgagaggagt tgagaggatg gcatttattg cggcttgctg
     5341 ttgttgctta gttcggagga agattgtttg cttcatcgaa aattcttctc tttaattcca
     5401 gcggctctga tagctttcat tactgcaatt accgttttgt ccttcccatc ctcatgcccc
     5461 atcgcataag caccttcttc accatctttc caaaagtcgt cattcgattc gggccagtcg
     5521 atatccagtt caatagctgc tcgcggtgcc tgccatatca cccaggcaaa ctcttttaat
     5581 tcatcgtctc ctgtgaactg gcttttgtct tttgaccacc agttttcaaa ctgtcggtag
     5641 ctatcgttca cttccctctc ccccaaataa aaaggcctgc gattaccagc aggcctgcta
     5701 ttagctcagt gatgtagatg gtcatttaat actccgtcac gttttcctgt cgccacgcct
     5761 cgtcatattc cgatttcggc atattggcga tgtagctata tggcgatcct gattcaagtt
     5821 gcaggaactg gtgcgattgc tcgtcaagga acaacgggac accaccttcc caaccttcgc
     5881 cgttacgttg tttttcaagc atcaaaacag atgccggaga tgccagtagc tgttcgtcct
     5941 tctctgacat cttttcacca ctctgaactc tctgtaacgc tctctcgcga gccttgttac
     6001 gccagatgat gaaaaggttg tctgtcaggt ctgttatcgc tccagagcct tttacgtcca
     6061 ttttcccggt tggtttttct tcgctgtctc cttttcgcga gtgagtaacg agaatgacgt
     6121 gggagtttgt tttgtttttg aagtcgcaaa tcgagtcaac aaacgccttc tgcccgttat
     6181 agtcatcgtc gcctatgcca catttcatca ggctgtcgat gatgaataac tggatcccgt
     6241 atcggcggcg agcgtagtcg aatatttcga tcagcctgtc ggctttcgcc gttccagtca
     6301 ggccaaacac ccaaagtctt tcgtcataaa atttaaatgc agagtcaatt tccagcactg
     6361 gcggcatctt gcagcacgtt gcctgacggg taagtcgctt aaggagaata ccaggcttca
     6421 gctcaagtga cgcgatgcac gtcttcacac cctgacgcat tgcctcaagt gccatatgcc
     6481 cgacaacctc ggtgttatgc gtcgccacat atcccctggt tacatacagg tggcgagggt
     6541 gatcgaccat aatacacaag cattctgcat tgccgatttt ttcaacattc ctgacgaata
     6601 ccccacagcc ctcgaatcga tgcgcagtta atctgccatt gagtcgtggt gaacggatgg
     6661 cctccctgat ttctcttgtc agtctgattc ttgcctcgta tgaatccatc ccatgccgct
     6721 tatcgtcctt gtatgtgtat gtcacgccag ttttaactcg cgtccggcat gacccgccga
     6781 gtgagttaac cagttgaaca accccattgc gcagttcttc acttgctgag gaaaaaacaa
     6841 gcgttccatc cttctcgaca tacccatccg tttcaagcag accacacagc atgcctatac
     6901 gcgttgattt atttgcggaa aaaaacacac gagggatgaa tttgtttttt gctgtgcacc
     6961 ccattagtcc ataacctcgc agggtctcca tgagtggatt tacctgacca cgcgccgttg
     7021 atatcagcca gtccttacca tctccagaga agttgtaatc aggcagttca gccttcatac
     7081 gctcgatcat gtatggctcg acgtttgaaa acttcacgct cccattgcta agactaccat
     7141 ccccgagaag ggagccgata acccatgcta atggctctga gtggtcgcca aagtcaccag
     7201 ttatttcagg aatcctaacg ccatttttgt gcctcttcgt ttcactcaac cgcttcaacc
     7261 cgaaggtatc tatcacgcgg cgtttctcgc ctttcgtgaa tccacgacta gtgacctccc
     7321 ataggtgatc gcctgcgcaa tcaacataac gaccatcttc aaatgtgact cggtaaacat
     7381 ctctaacacc ctgcgggaat atcccagtga cagttgacgg attgccgtct actgacgcca
     7441 cctgatcgcc aatttttaca ttcccgtgag tagtccatgt cccatcagct aaaagaatag
     7501 gctcattcag tgggcatgcc tttccgtgac cgttcacacc attgaccagc gtcaactctg
     7561 cctcacggaa ctggaattta tctgccagag attcccacgg tggattaaac agatactgct
     7621 gcttgccgta gaaagcgttg atagtgtcct ggtaaaactc tcgcgcactg tagagttctt
     7681 caggatcgaa gtaggatgcc gtgccgatgt actgccagat ttcatcctcg gtaacaccgt
     7741 tcatcaggca ttcgttgatg tctttgtacg gcagagtaac aagacggcaa cgatgttcac
     7801 cgagtcggct tgcgatttcc cttgcggctt cacgaccaac atcatcaacg tccatcgaga
     7861 tgaatatttc ctcaaacctg tcgaggttat gatactcaaa ctcaatccac tgttgcttag
     7921 cgcctttccc gccaccaaac ggcacggata acgccgagat gccgtattgc gcatagctca
     7981 tacaatcaat ttcgccttcg caaagcacaa ccgccctcac gccagcgtcc agagcctgcc
     8041 atccgaacag gcaaggttc
//
LOCUS       DAJTYF010000153         7954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTYF010000153 DAJTYF010000000
VERSION     DAJTYF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.153"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            59..460
                     /locus_tag="OO590_003665"
     CDS             59..460
                     /locus_tag="OO590_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6638875.1"
                     /translation="MSSNFCERLEQWGNKSVNNGEERAIAVERIKEAYNSNMASLDLS
                     YLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSVNKINTITFPKEPKLEN
                     IYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            570..1901
                     /locus_tag="OO590_003666"
     CDS             570..1901
                     /locus_tag="OO590_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6638876.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYIPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2225..3613
                     /gene="narU"
                     /locus_tag="OO590_003667"
                     /pseudo
     CDS             2225..3613
                     /gene="narU"
                     /locus_tag="OO590_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            3695..7328
                     /gene="narZ"
                     /locus_tag="OO590_003668"
                     /pseudo
     CDS             3695..7328
                     /gene="narZ"
                     /locus_tag="OO590_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            7325..>7954
                     /locus_tag="OO590_003669"
     CDS             7325..>7954
                     /locus_tag="OO590_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6638877.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIV"
BASE COUNT         2131 a         1967 c         1842 g         2014 t
ORIGIN      
        1 ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat
       61 ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga
      121 agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct
      181 tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt
      241 aaatctggaa aacaattgtc taacttgtct tgacttagct gacattgcca gtctcgttaa
      301 tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga
      361 aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt
      421 ggttaatctg gaagcgcaaa ataacagcct aaaaaaatga tatttctgat agttataaac
      481 tgaaatttct taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat
      541 ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc
      601 ccaagatgaa aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca
      661 ctacaaaact ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg
      721 atcaactaat atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc
      781 cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag
      841 tgaccattga tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca
      901 cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac
      961 tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa
     1021 atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt gatatagata
     1081 ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag
     1141 aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg
     1201 caggtaataa aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac
     1261 acaatgtctt atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa
     1321 ttaaaatatt ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt
     1381 tgaaatatat aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg
     1441 acaataataa aataacccac attgatacta ataatacatc cgatattgga actttattcc
     1501 ccataataaa acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc
     1561 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
     1621 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg
     1681 aattgtttca ctatactatt aaaaaagcat atatcccata taatatacac acttacaaga
     1741 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa
     1801 acaatactta ctataataac gattattaca atcaggcaat aagagaggaa ccgatagact
     1861 ttctggacag atcgttttcc tccaattcat ggccttttta gtgaataaat catcactaat
     1921 tctataagtg tctggtcttg ttgagaatat agttaatatg taacccttta gattcacgat
     1981 ctgaagggtt gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt
     2041 tagttacatt tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg
     2101 ctttcttatc tattctttca atcagcactt cacccaattc atccttaatt ctaataactg
     2161 gctattgcgc cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa
     2221 caatatggca ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga
     2281 aaatccggcc ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc
     2341 agtcagttgt ctacttcttg ccttctgtgt ctagatgcta tttagcgcag ttaccgttaa
     2401 tctcaataaa atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc
     2461 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg
     2521 acgccaatgg ccggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
     2581 tgccgtgcaa aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
     2641 ttttgcaggt gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
     2701 caaacaaggg agcactcttg ggattaatgg cggattagga aacttaggtg taagtgtaat
     2761 gcagctggtt gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg
     2821 cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
     2881 gctactggcg attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg
     2941 cgcctcaatt gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct
     3001 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc
     3061 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttattgg
     3121 tgccatcgcg cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac
     3181 gttgatcaac tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg
     3241 cacaggctcc ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgaccgc
     3301 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
     3361 ttatcgggtg aagatgaaag gcggtagtga tgagcaagct cagagagaag ccgtcaccga
     3421 aacggcggca gcgctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc
     3481 gcaggcattt ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt
     3541 tttaatcttc tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt
     3601 cagccaaaaa taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca
     3661 gatcccaatt cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg
     3721 ctacttcaaa caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa
     3781 ccgcgactgg gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac
     3841 ccacggcgtt aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt
     3901 gacctgggaa atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga
     3961 acctcgcggc tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct
     4021 gaaatacccg ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca
     4081 cagcgatccg gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta
     4141 caaacaagtg cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct
     4201 gattgccgcc gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt
     4261 ctcgccgatc ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct
     4321 gcttggcggc acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc
     4381 gatgacctgg ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta
     4441 catcatcgct tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac
     4501 cgaagtacgc tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc
     4561 caaattgtgc gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc
     4621 aatggggcat gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa
     4681 ctactgccgc cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg
     4741 tagctacgtt cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag
     4801 caacaatccg cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa
     4861 cggttcgatt ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc
     4921 cggtacggaa accgaattgt cgttaaccct gctcggtcaa catgatgctg ttgcaggcgt
     4981 ggccttcccc tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc
     5041 ggtgctggtg cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc
     5101 agtggtcagc gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga
     5161 tgaaaacagc gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca
     5221 aattaccggc gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc
     5281 ccataaaacg catgggcgct caatgattat cctcggcgca ggtgttaacc actggtatca
     5341 catggacatg aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca
     5401 aagcggtggc ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg
     5461 gttgccactg gcctttgcgc tcgactggaa ccgaccaccg cgccagatga acagcacctc
     5521 gtttttctac aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact
     5581 ttcaccgctc gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc
     5641 cgaacgtatg ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa
     5701 agctgaagcc gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc
     5761 gggcgattta cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt
     5821 gtttgtctgg cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa
     5881 gtatctgctg gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat
     5941 caaaccggaa gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt
     6001 gacgctcgac ttccgcatgt ccagtacctg cctgttctcc gatatcgtcc tgcccaccgc
     6061 cacctggtat gaaaaagacg atatgaacac ctcggatatg catccattta ttcatccgct
     6121 ttctgcggcg gtcgatccgg cctgggaatc acgcagcgac tgggaaatct acaaaggtat
     6181 tgccaaagca ttttcgcaag tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt
     6241 acaaccactg ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg
     6301 gcgcaaaggc gaatgcgatc tgatcccggg taaaaccgcg ccgaatattg tggcggtgga
     6361 gcgcgactac cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact
     6421 tggcaatggc ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa
     6481 actcaattac accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat
     6541 tgacgcttca gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa
     6601 agcgtggcag gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa
     6661 agaggacgag aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag
     6721 ccccacatgg tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt
     6781 tcatgagtta attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc
     6841 gtggatgcgt gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag
     6901 cgtcagtgag atgcgccaga taccgccaaa cggcttccag gaaaaagcac ttaacttcct
     6961 gacgccgcac cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac
     7021 gctctctcgc ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc
     7081 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     7141 actcggtcac ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     7201 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     7261 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     7321 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg
     7381 ccatacctgt tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc
     7441 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     7501 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg
     7561 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga
     7621 tgattactac gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa
     7681 acatatccct actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat
     7741 ctggggccca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     7801 caacttcgag gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta
     7861 cctgccgcgc ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg
     7921 cgctatctac aaacgcgaag aagacggcat tgtg
//
LOCUS       DAJTYF010000154         7710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTYF010000154 DAJTYF010000000
VERSION     DAJTYF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.154"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(1..367)
                     /locus_tag="OO590_003670"
                     /pseudo
     CDS             complement(1..367)
                     /locus_tag="OO590_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(707..1261)
                     /locus_tag="OO590_003671"
                     /pseudo
     CDS             complement(707..1261)
                     /locus_tag="OO590_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1404..1526
                     /locus_tag="OO590_003672"
                     /pseudo
     CDS             1404..1526
                     /locus_tag="OO590_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1788..2354)
                     /gene="ipgB2"
                     /locus_tag="OO590_003673"
     CDS             complement(1788..2354)
                     /gene="ipgB2"
                     /locus_tag="OO590_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6638878.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2636..2950)
                     /locus_tag="OO590_003674"
     CDS             complement(2636..2950)
                     /locus_tag="OO590_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638879.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3028..3138)
                     /locus_tag="OO590_003675"
                     /pseudo
     CDS             complement(3028..3138)
                     /locus_tag="OO590_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3462..4139)
                     /gene="ospD1"
                     /locus_tag="OO590_003676"
     CDS             complement(3462..4139)
                     /gene="ospD1"
                     /locus_tag="OO590_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6638880.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4510..4647)
                     /locus_tag="OO590_003677"
                     /pseudo
     CDS             complement(4510..4647)
                     /locus_tag="OO590_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4729..5027)
                     /locus_tag="OO590_003678"
                     /pseudo
     CDS             complement(4729..5027)
                     /locus_tag="OO590_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5616..5775)
                     /locus_tag="OO590_003679"
                     /pseudo
     CDS             complement(5616..5775)
                     /locus_tag="OO590_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5779..7008)
                     /locus_tag="OO590_003680"
                     /pseudo
     CDS             complement(5779..7008)
                     /locus_tag="OO590_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7028..7375)
                     /gene="tnpB"
                     /locus_tag="OO590_003681"
     CDS             complement(7028..7375)
                     /gene="tnpB"
                     /locus_tag="OO590_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6638881.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7375..>7710)
                     /locus_tag="OO590_003682"
     CDS             complement(7375..>7710)
                     /locus_tag="OO590_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6638882.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQIL
                     IREIKGSSH"
BASE COUNT         1984 a         1794 c         1685 g         2247 t
ORIGIN      
        1 tgacagtgtt gttgcgcctt gctgccaacg aagctccact gcccatcaag ttcacagata
       61 agggcaacat cagcgtgagc gactggcgca gatgttatcc gccggggcgg gagcttttta
      121 atgtccgtat gacggtattg atgccgattt tcagcgtacg ggcagtatcg cgaactccgg
      181 caccgttgaa agccatttca gtgatttggt ttttaactcc cggtttacgg gcctcgtaac
      241 agtaagtgag ctgaaacacc cggtgacagt cacggcaacg gaatcgatca tgtccttttg
      301 ggttctgccc gtgacggtaa acctgaacgg actggcaacg ggggcaatga aagttaactg
      361 tgaccatgaa agaaccttaa aagcgggtat tataacccct aattcaacta attggaggca
      421 ttgtcatttt taatctatgg atacacttaa aaatcctctc accttacgtt aaaaaccttc
      481 tcatgtttag agttttttat tatgtttatc ataatgatat gacgatctga gtttttaaaa
      541 tcaagaagaa aagataagtt ttttaaatgc acagtgcctg tggataactt tgcgtaaatc
      601 cggaggacag atcactctga agcaagacaa cagcatctga aaacaatatg ctcacgtttc
      661 aaccacaggg cgtaagtgat cctacccacg taatatggac acaggcctaa gcgaggttct
      721 ggttttcaaa ttgttccgga ctgaggccgc cacaccaact gtgccgtcgc cagcgattgt
      781 aatcacattc gatataatta aacactgttg cccgcattat ttcccggctg ataaagtgtt
      841 ctccgtggag acattccact ttcagcgaat gaaagaagct ttccacgcag gcattatcgt
      901 agcagcagcc ctttgcgctc atactgccac gcagattatg ccacttcagc agcgcctgat
      961 aatctgctga acagtactga cttcctcggt ccgagtgaac gataacgttc cggggtctct
     1021 tacgccgcca cagcgccatc tgcagggcat cgcaggccag ttgcgccgtc aggcgtggtg
     1081 acatggacca gccaataacg gcacgtgacc acaggtcaat gaccacagcc agatacagcc
     1141 agccttcatc tgtacgtaag tacgtgatgt ctcccgccca cttctggttc ggaccactgg
     1201 cgtaaaaatc ctgctccagc agattttctg acacaggcag gccatgtgcg cggtaactga
     1261 ccgtattgct gggtaagatc aatttgctca ctggtattta ctacgtgcat cgaatgagtg
     1321 atcaagagcc gtatgacgcg agagtgtcac gtacggttcc gtaagcagcc ggagagtgaa
     1381 actctctccg gctgactcga ctcgcaggtt ccgacagcgg tgacgaacat gcagcggtgt
     1441 tgttctcgct gatcggcaca tgccgtctga acaatgtgga gccagaaaaa tggctgcgtt
     1501 acgttattga acatatccag gactgaccgg caaaccgggt acgcgatctg ttgccttgga
     1561 aagttgatct gacttctcag taaatatcaa tatgattctg acgagccgct tacctttgag
     1621 cagagaaagg ggagagggat cccacatcca tgagctagga aaatacaaaa actggctata
     1681 caacaattca atatattagg acttacttgc gaggagaaat atagcaccgc tcaggcaatt
     1741 cctgggtatt tacttttctt agtttcttta ctcgggatgt tctgtcatca gaaaggcgat
     1801 tctaaatttg taatatagtc actcatattc ccacgtaatt tctttagatc aatatattgc
     1861 acatttttct caaaaaactg atcggaaata agatttgcaa cattgcttgt tgtatcattt
     1921 ttaacgcttc tattcatccc ttcacaaagt tcgtccattt tctttttgaa ataactgctt
     1981 cctgatacaa gatgatttat ggaactttgt gcagcattga tgtctagaga aatgcgataa
     2041 tctttctcaa cctgtttaaa tatctgttta cggagattat cgccgatttt tcggctgtgt
     2101 tctgcacaaa acttatcaat acattggttc accacccggg atacgtagac ttttttttca
     2161 tcatttttcc aatctgtaat attttcaccg atttgttctg aaacagagct caaaatacta
     2221 gtatttattt tggttgatga tatttttact atccttttcc ccattgtaca gcggataatc
     2281 tcatccacct ttcctgaaaa atatcttttg tgagataaac tgattccaaa attattaaaa
     2341 gatgttccaa gcattataat cactttgtca tgtaaaatag taacattgtc attattttag
     2401 ctgattcaaa caattctgta aatgctttga gttctttact cccttggctc aatgatggac
     2461 cgtctggaat cccagagact aaactctctg agaaagagag tatgaaaaaa cttggggcag
     2521 tccctaaatg aagggaccaa cagcggtaaa ataaccggca tcattcataa tgatgccctg
     2581 ttagttgtag ttatgaagat ttacgtcatt aaaattgctg ttcgtagtgt cagtcctatg
     2641 gtctggcatc agctgaggat cgtagccgat acatcactgg cttcacgaca ttcgtgttga
     2701 ggctattcat gaaaactcca cgctgaaaac gccgttttgt ataaacggtc acggtatgcc
     2761 cggagccact gttgctgatg aattcgataa aaccttggcg tttcttgaag ctatcgttaa
     2821 tgcggataat gaaacaacga ttggtgagat tcgctctttt gccgacgccc tggacgctgt
     2881 ccggttcaat cgcaacaaaa tcaaccggca gctgagcaaa cccaaccttg cctctctggc
     2941 gctggagcat gaagtgattt ggctgggacg tagccgctga cagcttctat aaaagtatga
     3001 tcctacccac gtaatatgaa cacaggctta agtgaggttc tggttttcaa attgttcagg
     3061 actgagacct ccacaccaac tgtgccgtcg tcagcgattg taatcgcact tgatataatt
     3121 aaacactgta tatttcattt gtggagtctc cagtctgagg gggctttatg cattccgtat
     3181 tgggcactat gatgccagaa atctgcaagg ctccacacct tcctgtattt cccttcctct
     3241 atcacagtgc actttactta atagggtggg atgtgttcat tacatttatt gtgtttttta
     3301 tgattgtttt tgcgtccagt gtcgttcgtc atgggaaact ggttctatgg gtagtatcgc
     3361 tcagtctagt taactgtttg tttcttttta gaaattaagc agatcacaca attcggggag
     3421 agatccttct aagtgcaatt ctttgcagct atttagcaca cttaaaacaa ctcttgtatt
     3481 ttgtcagaaa taatattaaa catgtcttgt tcattattgt taagtctgtc tgctatttct
     3541 gtaagtttta tcccactatg atggcatata tttatatatg ccatcatagc ctcgatattc
     3601 cctttccctg cggccagaaa taatcctggt aacccctcat tatctcttac atccaatagt
     3661 ttaatcgttt cttttggtga taaatcaaaa gttttaataa aatctccata agcttgaatc
     3721 gcagcggaat gaccttcttg aagagctatg aacaaagcag attccccagc attatttcgt
     3781 gctgcaagaa cttctagttt ttcctctttg ggtatttcgg ctgccaacag cgattcaccg
     3841 tatttacgcg ctgcatcggc atttccatgc caaatcaatg aaaacaaatt atggcggtta
     3901 tactccttat tattctttgt cggaatggat atgctctgta ttttggccaa ttttctgaaa
     3961 gaaacaccac tatatccccc ccctgatttt tcttcattga tggcgtggga tatagcggta
     4021 tttgtcacgt taatgatatt atttttcatt cctttatttt cattagcagt attgctgcct
     4081 attattaggt gcatattttt gttgtttgct ggatgtaatc catagttatt tattgacatt
     4141 tttgttcatc ctttttgcta aaaaaaacaa taacctattt ttgtaatgta atagatatta
     4201 gtctattgct aatctaaaaa aaccattgcc acattgaatg gctatattca ccaataaatt
     4261 ctttcgattt cgtatattga catatcgtag atatatctta tccctaagat tcaataatcg
     4321 agtccgttta ccattctgtg caagcgcctt cactcagaag tgaccgtcaa ggcgatcacc
     4381 aaacttgata ataaagcagc tcattgccat acgccagtcc ttaaggggcg ttgtccattt
     4441 ctgtgatgct gcctagattt aactgccagc tccggcttat ctacatctaa atatgtgagc
     4501 ctaataccgc tacactttgt ccagactatg tttttctccg ggaagtgggc taaccgtctc
     4561 cataaagtga cgaacttttt tcaacagtgg ccatattgaa cggcactgat gattacgggt
     4621 tattgtctca tgaagagccg gccacatcac tgacagcagt gacttcaggc cttcagcacc
     4681 agacagcctg aaccagttaa tccatcgccc gacagattta gtaatctagg gctggttttt
     4741 cttcagctgt tgcggttcga aagtgccatt acgatcacgc agagtacgga tttccagagg
     4801 ttcatctcca gtagtgataa tcttcgttaa gcaaccattg cgggaattgc tgccagatct
     4861 aagctgattt ttctcgcacc caagatgatg agtcatatta gcattgagta cggtttcatt
     4921 ttcagcatcc aatcaaactg actgagatct tcaggatttt tgagactttt ggctagttca
     4981 ttagctaaag cccgcaactt attagatgtc aactgttttt cattcataaa ttcaccttca
     5041 tttgatatca aattaaatag ttcaacatca gagaattata caaacttatg tacaggctcc
     5101 tatgatcttc gaatcctgaa gattatagtg aaaataagtt ttcacgttga aatcccacat
     5161 agttagtata tgataaataa tcatggatga cctgattaac ctcttggggt agtgctttta
     5221 tagaagaata aatattctac taatttaata catcacctaa aatagtattg ttttttatgg
     5281 ttggttgaag gtcgtggtga tgatgttagt ccttttgaga atattccagt gccagatctt
     5341 acttataata aaggggcttg gcagtttgga taaactattg ttcttattcc actaatacaa
     5401 actttttgta aagtatatta aatatacttc atttcatact gaaatccccg gaagtattca
     5461 atcaaacaat tacaccataa aaggaagagg gggccctaat taagttgtca agcatgttta
     5521 tgatacctgc agggtataaa ttttctggct gcaagcagac acatataaaa cgtctttatt
     5581 gatcctaccc acataatatg gacacagtcg taagtgaggc tctggttttc aaattgttcc
     5641 ggactgagac caccacacca acggtgccgt cgccagcgat tgtaatcgca ctcgatataa
     5701 ctaaacactg ttgcccgcac tatttcccga tggataatag tgttctccgt ggagacattc
     5761 cattttcagc gaatgaattt tcatctgttg ctgtatccag tcatacagtg actgcatcag
     5821 tggcgcggct ctggcttttc ttgccgccag acgctgttct gctgaacatc cccggacttc
     5881 tgcctcgatg gcatacagtt caccgatacg ctgcagggct tccgtggtga tgtcggttgg
     5941 cactcttgca tgcacatcgt ggatttttct ccggacatga gccatacatg cggcttccgt
     6001 tattctgccg gattcgtaca acgcccggta accaccgtaa gcatcggcct gaagcactcc
     6061 gctgtaaccg gccagatgat tctgtggatg gatacctttc cggtccggtg agtacgcgaa
     6121 ccagaccgcc ggaggcattt ccgaaccggc gttacggtca tcacgaacgt agacccacag
     6181 ccgggcagtc cttgttttac cgctgcccgg ctcctggacc ggaacgggga tatcatcggc
     6241 atggacttta ccgggcatca gcacatactg gcgcaggatg tcatacagcg gttccagcag
     6301 ttcagcaaca gcacctgtcc agcgccccag tgtggcacgg ctcagctcca cgccctgacg
     6361 acggtatatt tctgactggc ggtataacgg cagatagtct gcatattttc cagcgacaac
     6421 atgggccaga agccccgctc cggcataact gcgtgcaatg ggttttgaag gcaccggtgc
     6481 ctgcacgata tggtcacacc ggcaacaggc cagtttcgga cgttgtgttt cgataacctt
     6541 aaaggcgctg ctgataagct ccagctgctc tgacacatca catcccagag aactgagttc
     6601 gccaccacag gccgggcagc attcctcttc cggacggatg acccgggttt cacggggaag
     6661 tgaggcaggt aacggtttac gggctgaaga ctggcgcagg ggagatggca gtatcgggtc
     6721 atattgctca cccagcgttt ccgccatttc ttcctgaagt gcgctgattc gctcctgtgc
     6781 ttccagtatc tgccgttcgg tttttgcgcg aagtttttct gagcttttac cgaactgcat
     6841 gcgctgcagt ttcgcaacca gcgccttcag ccggttgatt tcggaagcat aagccgccac
     6901 ccgctgtgag agcaggcggt tgtatttcgc catctggcgg atggtgtcct gttgcgtctg
     6961 caacagtgcc cgcaggcggg cattctcatg agaaagtgag gtgtccatat cctcacttta
     7021 caacgggtta tatgcggatg ccagcgcgtt ccgttcgttt cgggtgtttc cagtcgatgc
     7081 cctcaagaag catggataac tgggccggag taaggtgcac cttgccgtca cgggtgaccg
     7141 gccagacgaa gcggccccgc tccaggcgtc tggtaaagag gcacagtccg tcactgtcag
     7201 cccacaacac ttttatctgg tcaccccggc gtccgcggaa gatgaacaga tgcccggaga
     7261 acgggtcatc cttcaggacg ttctgaactt ttgaggccag gccgttaaag ccatttcgca
     7321 tatcggtgat acctgcaacc agccagatac gcgaacctgc agggaaagat atcatcagtg
     7381 gctgctccct tttatttcgc ggataagtat ctgtaataac gccggcgtca gtttaccttt
     7441 aagcctgaga gttccggccg gcagaaccag ctcacagcac agactgtcgg acggtgtatt
     7501 tatctgctcc ggcgttaacg tcacgggaag cagtgcaggc atattttttc cggaaggcag
     7561 caggccacct ttccggtatt gatggcgcca gttgaagagc aggttatcgt tgattccgtt
     7621 ttcacgggcg atctgcgcca cacaggctcc gggctgcagt gactgctcca ctaaagcgat
     7681 tttaaattca taaggaaaat taggccgccg
//
LOCUS       DAJTYF010000155         7623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTYF010000155 DAJTYF010000000
VERSION     DAJTYF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.155"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(434..640)
                     /locus_tag="OO590_003683"
     CDS             complement(434..640)
                     /locus_tag="OO590_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6638883.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            953..1042
                     /gene="kdpF"
                     /locus_tag="OO590_003684"
     CDS             953..1042
                     /gene="kdpF"
                     /locus_tag="OO590_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6638884.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1042..2715
                     /gene="kdpA"
                     /locus_tag="OO590_003685"
                     /pseudo
     CDS             1042..2715
                     /gene="kdpA"
                     /locus_tag="OO590_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
     gene            2738..4785
                     /gene="kdpB"
                     /locus_tag="OO590_003686"
                     /pseudo
     CDS             2738..4785
                     /gene="kdpB"
                     /locus_tag="OO590_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4794..5366
                     /gene="kdpC"
                     /locus_tag="OO590_003687"
     CDS             4794..5366
                     /gene="kdpC"
                     /locus_tag="OO590_003687"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6638885.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAVAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5359..6342
                     /locus_tag="OO590_003688"
                     /pseudo
     CDS             5359..6342
                     /locus_tag="OO590_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            6342..7623
                     /locus_tag="OO590_003689"
                     /pseudo
     CDS             6342..7623
                     /locus_tag="OO590_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141249.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
BASE COUNT         1646 a         1952 c         2173 g         1852 t
ORIGIN      
        1 ataacatctt attggattag atatgttaac aattaattat tcttaatata tcaataaagt
       61 atatattact ctctttacta tctgttttta ttgcggtagc gatactaata acaaaattcg
      121 gaatttattt agatgctgga attagaaaaa gggatgacgg ctttctcacc ttaccctgaa
      181 gtttcacgaa agtgtgtaag gaagtatgca atctaactgg agatttctga tgatctagtc
      241 gcttagagcg acaatgaagg tgaccgcatc ctgggtagcc ctatgaaagg atactattga
      301 aacgtgatgc taaaacagtg atgaaatgaa taatacgatg gaaacgtctt ccatgttaat
      361 tatcatggaa gacgttgatg ttgaacacct aaaccagccc cctgatacga ggctggctta
      421 agcgcgcagg caatcagtaa aaatcaccgg ttgccttttc ggcctgatcc atccacaccg
      481 gtttatcgct ggttttcgac cagacgcggt gcaggtagct gtaaaagcgg gcgcggtctt
      541 tccagaaaaa catgaaaggc agcgccagaa cgcccgctgc aaccgcatat gtacggcgta
      601 aaaagataag ccatgcagga tattctctgt agagttccat acttttctcc cccttttgta
      661 tctacccggt gaatagcacc ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc
      721 ttttgtgtaa ttttaccact catccgacca cttatttttg cttattgatg gtttatttac
      781 attcacactg taattaaatt acacaaaagt taaattaata ctaaccatca gttaaatcat
      841 ggcctttgcc atttttatac tttttttaca ccccgtccgc agatttttgc gaaatctttg
      901 cagccagaat tctactcttc cggtatcact tttaggctac tggaggtgca ctgtgagtgc
      961 aggcgtgata gccggcgtgt tgctggtgtt tttattactg ggttatttgg tttatgccct
     1021 gatcaatgcg gaggcgttct gatggctgcg caagggttct tactgattgc cacgttttta
     1081 ctggtattaa tggtgctggc gcgtccttta ggcagcgggc tggcgcggct gattaatgac
     1141 attcctcttc caggtacagc gggcgttgaa cgtatactct ttcgtttacc tggcgtctct
     1201 gaccatgaga tgaactggaa gcaatatctt tgtgccattc tcggcctgaa catgctgggg
     1261 ctggcggtgc tgttttttat gttgctcggt cagcactatc tgccgcttaa tccatagcag
     1321 ttgccggggc tgtcgtggga tctggcgctg aataccgccg tcagctttgt cacgaatacc
     1381 aactggcaat cttatagcgg tgaaaccacg ttgagctatt tcagccagat ggcaggctta
     1441 acggtacaaa actttctttc tgccgccagc gggattgcgg tgatttttgc cttcatccgt
     1501 gcgtttactc gccagagcat gagcacactc gggaatgcct gggtcgatct gctacgcatc
     1561 acgttatggg tgctagtccc tgtggcgttg ttaatcgcac tgttttttat tcaacaaggt
     1621 gcgctgcaaa actttctgcc ctatcaggca gtgaataccg ttgaaggagc gcaacagctg
     1681 ttacccatgg gaccagtagc ttctcaggaa gcgatcaaga tgctcggtac taacggcggt
     1741 ggcttcttta atgccaactc gtcgcatccg tttgaaaacc caaccgcact gaccaacttc
     1801 gtgcagatgc tggcgatctt cttgatccca acagcgctgt gctttgcctt tggtgaagtg
     1861 acgggcgatc gccgccaggg gcgcatgttg ctgtgggcga tgtcagtgat ttttgtcatc
     1921 tgcgtaggcg tggttatgtg ggcagaagtt cagggtaatc ctcatctgct ggcgctcggc
     1981 gcagacagca gcatcaatat ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc
     2041 ctgtttgcgg tcgtgactac ggcggcgtcc tgtggcgcgg tgattgcgat gcatgattcg
     2101 tttaccgctc tcggcggcat ggtgccgatg tggctaatgc aaattggtga agtggtgttc
     2161 ggcggtgtcg gttctggtct ttacggcatg atgctgtttg tcctgctggc ggtgtttatt
     2221 gccgggctga tgattggtcg tacaccggaa tatctgggta aaaaaatcga cgtacgcgag
     2281 atgaaactga ctgcactggc gattctggtt actccaacac tggtactgat gggcgcggcg
     2341 ttggcgatga tgaccgacgc cggacgtagc gccatactta accctagccc gcatggtttt
     2401 agcgaagtgc tgtacgccgt gtcgtccgcc gctaacaaca acggcagcgc ctttgccgga
     2461 ttaagcgcca actctccgtt ctggaactgt ttactggcgt tctgcatgtt tgtcggtcgc
     2521 tccggggtga ttatcccggt gatggcaatt gccggttcgc tggtgagtaa aaagagccaa
     2581 cccgccagct ccggcacgct gccaacgcac ggcccgctgt ttgttggcct gttaatcggc
     2641 accgtgttgc tggttggcgc actgaccttt atccctgccc tggcgcttgg tccggtggcg
     2701 gaatatctct cctgatgata ttgagtgagc actgaatatg agtcgtaaac aactggcgct
     2761 attcgaacca acacttgtcg ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca
     2821 ggcgcaatgg cgcaatccgg tgatgtttat cgtctggatc ggcagtctgc tgaccacctg
     2881 tattagcatc gcgatggcaa gcggtgcgat gcctggcaat gcgctgttta gcgcggcaat
     2941 tagcggctgg ctgtgggtca ccgtactgtt cgctaatttc gccgaggcgc tggcagaagg
     3001 ccgcagtaaa gcgcaggcca acagtcttaa aggggtgaaa aaaactgcct ttgcccgcaa
     3061 gctgcgtgag ccgaaatatg gcgctgcggc ggacaaagtt cctgccgatc aactgcgtaa
     3121 aggtgacatc gtgctggtag aagctggcga tattatcccc tgcgatggtg aagtcattga
     3181 aggcggcgca tcggtcgatg aaagcgccat caccggggaa tcggcaccgg tgatccgtga
     3241 atccggcggc gattttgcct ccgtcaccgg cggcacgcgt attctttctg actggctggt
     3301 gattgagtgt agcgttaacc ccggtgagac atttctggat cggatgatcg cgatggtgga
     3361 aggcgcacag cgacgcaaaa caccgaacga gattgccctg actatcctgc tgattgctct
     3421 gaccatcgtc tttttactgg caaccgccac gctgtggccg ttttccgcgt ggggcggtaa
     3481 tgccgtgagt gtaacggtac tggtggcgct gctggtctgt ctgatcccaa ccactattgg
     3541 tggcctgttg tcagcgatcg gcgtcgccgg gatgagtcgg atgctgggcg cgaatgtgat
     3601 tgccaccagc ggacgtgcag ttgaagcggc aggtgacgtt gacgttctgc tactggataa
     3661 aaccggcacc atcacactcg gtaaccgtca ggcgtcggag tttatccccg cgcagggcgt
     3721 ggatgaaaaa acgctggctg acgccgcaca actggcttcg ctggctgatg aaacgccgga
     3781 aggtcgcagt attgtgatcc tcgccaagca acgttttaac ctgcgcgagc gcgatgtgca
     3841 gtcgctccat gccacctttg taccgtttac tgcgcaaagc cggatgagcg ggatcaacat
     3901 cgacaaccgc atgatccgta aaggttctgt cgatgccatt cgccgccata ttgaggctaa
     3961 cggcggtcac ttccctgccg atgttgatca aaaagtcgat caggttgcgc gtcaaggagc
     4021 cacgccgctg gtggtggtgg aaggttctcg tgtgctgggc gttattgcgc tgaaagatat
     4081 cgtcaaaggc ggtattaaag agcgcttcgc ccagctgcgc aaatgggcat taaaacggtg
     4141 atgattaccg gcgataaccg tctgactgcc gccgcgatag ctgcggaagc gggtgtcgat
     4201 gattttctcg ccgaagcgac accggaggcc aagctggcat tgatccgtca gtatcaggcg
     4261 gaaggtcgtt tggtagcgat gaccggcgac agcaccaacg atgctccggc gctggcgcag
     4321 gcagatgtcg cggtggcgat gaactccggc acccaggcgg cgaaagaggc gggcaatatg
     4381 gtggatctcg actctaaccc gaccaagttg atcgaggtgg tgcacattgg caaacagatg
     4441 ctgatgaccc gtggctcgct gaccaccttc agcattgcca acgatgtggc gaaatacttc
     4501 accattattc cggcggcgtt cgcggcaaca tatccgcagt taaatgcgct gaacatcatg
     4561 cgcctgcatt cgcccgactc cgccattctc agtgcggtca ttttcaacgc cttgattatc
     4621 gtctttttga ttcctctggc gttaaaaggc gtgagttata aaccgcttac cgcttctgcc
     4681 atgttgcgcc gtaacttatg gatttacggt ctgggtgggc tgctggtgcc gtttattggt
     4741 atcaaagtca ttgatttact gctaaccgtt tgtagtctgg tgtgaggttt accatgagtg
     4801 gattacgtcc ggcattatcg acatttctct ttctgttatt gattactggc ggcgtttacc
     4861 cgctgctgac caccgcactg gggcaatggt ggtatccctg gcaggccaac ggttcgttga
     4921 ttcgtgaagg tgatacggtg cgcggttcgg cattaatcgg gcaaaatttt accggcaacg
     4981 gctattttca tggtcgcccg tcggcaacag cagaaatgcc ctataatcca caggcttctg
     5041 gcgggagcaa tctggcagtc agtaacccgg agctggataa acagatagcc gcacgcgttg
     5101 ccgcattacg ggccgctaac ccggacgcca gcacgaacgt tccggttgaa ctggtgacgg
     5161 cgtcggcaag cggactggac aataacatca ccccgcaagc ggtggcctgg cagatcccac
     5221 gcatcgcaaa agcgcgtaat ctcagcgttg aacagctcac gcagctgatc gcaaaataca
     5281 gccaacaacc gctggtgaaa tatatcggcc agccggtcgt caacattgtc gaactcaatc
     5341 tggcactgga taagcttgat gaataacgaa cccttacgtc ccgaccccga ccgtctgctg
     5401 gaacaaactg ccgcgccgca tcgggggaag ctgaaagttt tcttcagtgc ctgtgcaggc
     5461 gtcgggaaga cctgggcgat gctggcagaa gcccagcgac tgcgggcgca agggctggat
     5521 attgtggttg gtgtggtaga aacccatggg cggaaagata ccgccgccat gcttgaaggg
     5581 ctggctattc tgcctctgaa acgccaggcg taccgtgggc ggcatatcag cgagtttgat
     5641 ctcgatgccg ccctcgcccg ccgtccggcg ctgatcttaa tggacgaact ggcgcacagt
     5701 aatgcgccag gttctcgtca tcctaaacgc tggcaggata tcgaagaact gctggaagct
     5761 ggcattgatg ttttcacaac cgtcaacgtt cagcatctgg aaagtctgaa tgatgtggtc
     5821 agcggcgtca ccggaattca ggtacgggaa accgtgcccg atcctttttt cgatgccgcc
     5881 gacgacgtgg tgctggtgga cttgcccccg gacgatctgc gccagcggct gaaagaaggc
     5941 aacgtctata ttgccgggca ggcggagcgc gccattgaac attttttccg caaaggcaat
     6001 ctgatcgccc tgcgcgaact ggcactgcgc cgtactgccg atcgcgttga tgagcaaatg
     6061 cgcgcctggc gggggcatcc tggcgaagag aaagtctggc acacgcgcga cgcgatcctt
     6121 ttatgcatcg gccataacac cggcagcgaa aactggtccg cgcggcggcg cggctggcgt
     6181 cacggctggg gagcgtctgg cacgcggtgt atgttgaacc ccctgccctg catcgcttac
     6241 cggagaaaaa acgtcgggca attctcagcg ccttacgtct ggcgcaggag ctgggcgcgg
     6301 agacggcaac actttctgat ccagcagaag agaaagcggt agtgcgttat gcccgtgaac
     6361 ataatctcgg caagattatt ctcggtcgcc cagcctcgcg ccgctggtgg cgtcgggaaa
     6421 catttgctga ccgactggcg cgcatcgccc ccgatctcga tcaggtgctg gtcgcgcttg
     6481 atgaaccacc cgcccgcacg attaacaacg cgccggatag ccgctcattt aaagacaagt
     6541 ggcgggtaca gattcaggga tgcgtggttg ccgccgcgtt atgcgccgtt atcaccttaa
     6601 ttgccatgca gtggctgatg gcgtttgatg ccgccaacct ggtgatgctg tatctgcttg
     6661 gcgtggtggt ggcgctattt tatggacgct ggccttcggt ggttgccacc gtcattaatg
     6721 tggtgagttt cgatctcttt tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc
     6781 aatatctgct gaccttcgcg gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg
     6841 ctggcgtgcg ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca cggcacttat
     6901 atgaaatgtc gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc gccaccagcg
     6961 aacaatttat tgcctctacg tttcatgccc gcagtcaggt gttgttgccc gatgacaacg
     7021 gtaaattgca gccgttaaca catccgcaag gaatgacgcc atgggacgat gccatcgcgc
     7081 agtggagttt tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac
     7141 cgtaccagat tttgccgcta aaaagcggcg agaaaaccta cggctggtgg tggtggagcc
     7201 ggggaatctg cgccagttga tgatcccgga acagcagcgc ctgctggaga cttttacgct
     7261 gttagtcgcc aatgcccttg agcggctgac gctaaccgcc agcgaagaac aggcgcggat
     7321 ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggca gcgctttcgc atgatttacg
     7381 cacgccgctt acggtgctgt ttggtcaggc agaaatctta acgctcgatc tggcaagcga
     7441 aggatcaccc cacgcccgcc aggccagcga gattcgtcag catgtgctga acactacccg
     7501 actggtgaat aatctactgg atatggcgcg aattcagtcc ggcggcttta atttgaagaa
     7561 agagtggtta acgctggaag aagtagtcgg cagcgcgctg caaatgctgg aaccgggttt
     7621 atc
//
LOCUS       DAJTYF010000156         7593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTYF010000156 DAJTYF010000000
VERSION     DAJTYF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.156"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            83..2263
                     /locus_tag="OO590_003690"
     CDS             83..2263
                     /locus_tag="OO590_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6638886.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2404..3687)
                     /locus_tag="OO590_003691"
     CDS             complement(2404..3687)
                     /locus_tag="OO590_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6638887.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3822..4892)
                     /locus_tag="OO590_003692"
     CDS             complement(3822..4892)
                     /locus_tag="OO590_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6638888.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(4870..5088)
                     /locus_tag="OO590_003693"
     CDS             complement(4870..5088)
                     /locus_tag="OO590_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR6638889.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(5173..5796)
                     /locus_tag="OO590_003694"
     CDS             complement(5173..5796)
                     /locus_tag="OO590_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR6638890.1"
                     /translation="MTSQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            complement(6118..6825)
                     /locus_tag="OO590_003695"
     CDS             complement(6118..6825)
                     /locus_tag="OO590_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6638891.1"
                     /translation="MAQLYGTDVVRIRQNHHENKSRFVEGVHFYKVVGDELRALKHKV
                     ALNYFVKSAPNARHLILWTERGAARHAKMLETDQAWDVFEKLEDGYFNQREKVANHPQ
                     PVPQPESSLDRARAMEIALNVADRLVNMFPNLSSESKQAAVAGVFNPIVGRDVVPLPA
                     ISEHYYSATEVGQRYGISANKVGRIANTYMLKTEKYGKWFIDKSAHNDKQVETFRYNE
                     AGVHKIEELIEGERKAA"
     gene            complement(6773..7207)
                     /locus_tag="OO590_003696"
     CDS             complement(6773..7207)
                     /locus_tag="OO590_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6638892.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIGAVVRNGCCAHPAKPS"
     gene            complement(7280..>7593)
                     /locus_tag="OO590_003697"
     CDS             complement(7280..>7593)
                     /locus_tag="OO590_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR6638893.1"
                     /translation="QAYANAEFIAAFNPNVALALLDERERNLQYIKSRDQENEEIALT
                     VGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAQTVTVKEVGD
                     A"
BASE COUNT         1762 a         1891 c         1858 g         2082 t
ORIGIN      
        1 tcctgcaact cgaattattt agagtatatc catttattat ctttctgcgc acttcacggt
       61 gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag
      121 taataacgaa ataattgccg aagaacattt caccggcgaa attaaaaaag aagaagcaaa
      181 aaaaggcact attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa
      241 acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg gtattgtaca
      301 gaccgctaaa gcgtccggaa tggaacgttt cccgctgccg tatgtgctga ccaactgcca
      361 taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc
      421 ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata
      481 tatgcgtgag atgatggctg gcgggggcaa aatgatcctc gggtcagaca gccacacccg
      541 ttacggtgca ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg taaaacagct
      601 gcttaatgac acctgggata tcgactatcc gggcgtggtt gcggtgcatc tgaccggaaa
      661 acctgcgccg tatgtggggc cacaggatgt ggcgctggct atcattggcg cggtgttcaa
      721 aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta gcgcgctctc
      781 taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt taagctccgt
      841 ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg gccaggatta
      901 ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag
      961 cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac
     1021 actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg aacgcgtggc
     1081 gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca
     1141 gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa
     1201 tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcggttt acccgtcatc
     1261 acagccggtg tttatggatc tagccaaaaa aggtgtggta gcagatttga ttggcgcagg
     1321 cgcaatcatc agaaccgcat tctgtggccc atgctttggc gcgggcgata cgccaatcaa
     1381 caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc
     1441 agccaatggg cagatgtcag cggtggcgct gatggacgct cgttctatcg ctgcgactgc
     1501 ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca acgtgccgga
     1561 gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc
     1621 aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat tgggtgcgct
     1681 gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtca ccaccaccga
     1741 cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcggg
     1801 gaatgtcagc gagctgacag aggtgtttgc gcgcattaag cagattgctg gtcaggagca
     1861 tattgatccg ctgcaaactg aaattggcag catggtctat gcggtgaaac caggcgatgg
     1921 ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg cgaatattgc
     1981 cgaggagtac gcgactaaac gctaccgttc taacgtcatc aactggggga tgttaccgct
     2041 gcagatggcg gaagtgccaa cctttgaagt gggggattac atttacatcc ctggcattaa
     2101 agcggcgctg gataatccgg gtacgacgtt taaaggttat gtgatccatg aagatgcgcc
     2161 ggtaacggaa attacgcttt acatggaaag cctgacggct gaagagcgcg agattatcaa
     2221 ggcgggtagt ttgattaact tcaataaaaa ccatcagatg taaaaagcgc catgtgaatg
     2281 taggtcgcat tcggcactta ttgtcggatg cgatgctggc gcatcttatc cgacctacaa
     2341 atcgcatcga accgtaggcc ggataaggcg tttacgccgc atccggcaaa tagttaatag
     2401 ctcttacttc ttcgcctctg caaccacttt gctgcccacg ccgcggttat tgtattccca
     2461 catgcggttg tagttagtgt cattcagatt gcgctgtatt tcgtcgttat catctacgct
     2521 gccggtatta cccgcaaacg gacgattaga gatcaccgca tcggcccacg gtttagccgt
     2581 gttaaaacct tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg tattagcatc
     2641 aacatccagc gagcggccca gttgcgccac gccatcaccg gaagcattga aacggctgtt
     2701 tacggcgagg aaaccgtaat agatgttaga cagcgtagcc ggtgcaaaca catatgcttc
     2761 ttgctgggta cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca ctgcgccgcg
     2821 accagaaacg atatccacat ccccttcaat atagctgttg gtcaccagcg tacgcggctg
     2881 acggttggtt tccagacggt tctgcacacc gctgttggtg acaaagaagg tgttctgacg
     2941 accgagaatg ttaacgttgt taatctgtac ctggtcaccg tcagtacgca gtgctaccgc
     3001 cggatggtta cctgcatcta cactatcgcc cagcgtgttt tcgatggtca gattttgcag
     3061 ttgcagtcca ttgttttgtg accagaagac cgcagaacac agaacaccga tactgtcgct
     3121 gcgtttgctc tggcagctat cgtacatata ccacgctggt ttacctggca tatatttgcc
     3181 gcgcgggttg acgtcgtgac gccagtcggc agggctcatg cccccatcaa gggaaagccc
     3241 aatcttcaca tcaatcggtt tttcacctgt accgtacaga gtgattccac ctggagcagc
     3301 agggacatac accgttccct gatactcacc aggcatcacg gcaatatact ggcgcttgtt
     3361 ggtgcgcttg ataattgccg catctaccgc cgcctgaatc gtggtatgcg ttacaccttg
     3421 agtacccgcc gggccgacaa caaagtcagg ttgcgcaggc agggttatcg gggacggatt
     3481 ccacgctgca gcacctggtg tcagggatgc aaaatagtgt tgcgcatcga aattcagcgc
     3541 ttcttttgcc gacagaatcg ggcgagaaga ggtaccaggc gcagtttgat cagaaggacg
     3601 ttgatcgggt ggtgttgagc tacaggcggt cagcgtcacg ccaaaagcca atgccagcgc
     3661 cagacgggaa actgaaaatg tgttcacagg ttgctccggg ctatgaaata gaaaaatgaa
     3721 tccgttgaag cctgcttttt tatactaagt tggcattata aaaaagcatt gcttatcaat
     3781 ttgttgcaac gaacaggtca ttatcagtca aaataaaatc attatttgat ttcaattttg
     3841 tcccactccc tgcctctgtc atcacgatac tgtgatgcca tggtgtccga cttgtgcccg
     3901 agaagatgtt gagcaaactt atcgcttatc tgcttctcat agagtcttgc agacaaactg
     3961 cgcaactcgt gaaaggtagg tggatcccct tcgaaggaaa gacctgatgc ttttcgtgcg
     4021 cgcataaaat accttgatac tgtgccggat gaaagcggtt cacgacgagt agatgcaatt
     4081 atggtttctc cgccaagaat ctctttgcat ttatcaagtg tttccttcat tgatatcccg
     4141 agagcatcaa catgcaatgt tgtagggatg gcaattttta cgcctgtttt gctttgctcg
     4201 acataaagat atccatctac gatatcagac cacttcattt cgcataaatc accaactcgt
     4261 tgcccggtaa caacagccag ttccattgca agtctgagcc aacatggtga tgattctgct
     4321 gcttgataaa ttttcaggta ttcgtcagcc gtaagtcttg atctccttac ctctgatttt
     4381 gctgcgcgag tggcagcgac cgggtttgtt gttatatggc cttcagctat tgcctctcgg
     4441 aatgcatcgc tcagtgttga tctgattaac ttggctgatg ccgccttgcc ctcgtctatg
     4501 tatccattga gcattgccgc aatttctttt gtggtgatgt cttcaagtgg agcatcaggc
     4561 agccccctcc ttattgcttt aattttgctc atgtaattta tgagtgtctt ctgcttgatt
     4621 cctctgctgg cgaggatttt ttcgtagcga tcaagccatg aatgtaacgt aacagaatta
     4681 tcactgttga ttctcgctgt cagaggcttg tgtttgtgtc ctgaaaataa ctcaatgttg
     4741 gcctgtatag cttcagtgat tgcgattcgc ctgtctcggc ctaatccaaa ctctttaccc
     4801 gtccttgggt ccctgtagca gtaatatcca ttgtttctta tataaaggtt agggggtaaa
     4861 tcccggcgct catgacttcg ccttcttccc atttctgatc ctcttcaaaa ggctacctgt
     4921 tactggtcga tttaagtcaa cctttaccgc tgattcgtgg aacaaatact ctcttccatc
     4981 cttaaccgga ggagggaata tcctgcactc gcgtacccat cgacgaactg tttcaaggct
     5041 ccttgggcgt cgctggcgtg cgttccactc ctgaagtgtc aagtacatcg caaagtctcc
     5101 gcaattactc gcaagggagc gagtattggt ttattttctc caacaaaaaa ggagccgaag
     5161 ctcctttgat gattaaaatt cgaattgtct cgcccgaagg cttttcagca ttggttttgc
     5221 ccgttcgaaa agggcgcttg cttggtcaag tcgtgtcgcc tcccttagca gtacatctct
     5281 gtttttcgtc accgtgtaaa aagtttcaaa cgcgatgtca tacagcttgc tcgtgtatga
     5341 tgagttcagt tccttcaata tcgggtacag acgtttgcag atgtcctgcg cattctccat
     5401 ctgtacctgc atatagcaaa ggaggatgat ttcttcgtct gtgaattgct gcgctggttg
     5461 catgctgcga agtttctttt cgcactcgat gaagtatcgg cgtatctggc ggcctttttc
     5521 gttacgctcg accatcgccg tttctttggc tgtatcgagg gtgaggtgat agtctttgct
     5581 acggcgatcg cctccacgtc ctttgatccc cctgttttgg tgaaccaaaa tgtaatcgac
     5641 atcaacggca aagccgtatt ctgttatgcg atttttaatc catgtagaga agtcacgacg
     5701 tacttccaaa aattcgtgta aatcacgggc attaacgaga agagttgttt cgttggatat
     5761 agtgccgttg aatacgggga tgagttgact ggtcattttt atgtcctttg catttgttca
     5821 gatagccctg tgttcagcag ggcggtcagg tacttgaaca ccgtgcaaag tcggcccgta
     5881 tccttaacct ttcggctgtt tttcggtata cgcgctaccc gaccatatct gaaaaatgga
     5941 cataaaaaat ccgcatgact gacgggtgcg gtttccgctt tgcaaggtgt gttcagcacc
     6001 atgaagcgga atatagcccc gttaatgcgg tgttgtcaaa tcatgtaggc cttatcttgc
     6061 tgtaagccgc gccattcggg cttttcccca catttgggga aaactatctt gtcaaaatca
     6121 tgcagccttt cgctctcctt cgatgagttc ctctattttg tgcactccag cctcgttgta
     6181 ccgaaatgtt tcaacctgct tgtcattgtg cgccgatttg tcgatgaacc acttcccgta
     6241 cttttctgtt ttgagcatgt aggtgttggc tatgcgtcct accttgttgg ctgaaatacc
     6301 gtagcgctgg ccgacttccg tagcagagta gtagtgctct gagatagctg gcagaggtac
     6361 aacatcgcga ccaacaatcg ggttgaacac tccagctact gctgcctgct ttgattctga
     6421 ggacagattc gggaacatat tcaccagtcg atccgccacg ttcaaagcta tttccatggc
     6481 tcgcgcacgg tctaggctac tttctggttg cggtactggt tgaggatggt tggcaacctt
     6541 ttcgcgctga ttgaagtagc cgtcctccag tttttcgaac acgtcccatg cctgatccgt
     6601 ttcgagcatc ttcgcgtgac gggcggctcc gcgctctgtc cagaggatga ggtggcgtgc
     6661 atttggggca cttttcacaa agtagtttaa agctactttg tgttttagcg cgcgcagctc
     6721 gtcaccaacg accttgtaga agtgtacccc ctcaacaaag cgcgacttgt tttcatgatg
     6781 gttttgccgg atgcgcacaa catccgttcc gtacaactgc gccaatgttt ggtgggtagt
     6841 tgtttttaaa gtcagctgct aacacatagc attgtcgctc tcccatttct ggcattcgct
     6901 cactacagct tatccaggca tccggagtta ccggcactgg ctgggcggta taaagcggtg
     6961 ttatatctgc ccgaaaatta catgctttat gcagccgcac ccaccgttcg acttctgctt
     7021 tgtcagaata cataccagtg aacgtgttat attcacggtc aatttgcgtg aaggttacct
     7081 tccacgccac cggctctgct gtcagcgatg ccagagcaat ttcataagca cggcgttcaa
     7141 cattgtctcg cacatccaaa ctgctgatac gctctttgat ttctttaatc agttctttgt
     7201 cgttcactta tgcctccgat tcccggatgt cacccaaatc aacgctacaa tgccaaccag
     7261 tgcaacgagc gcaaaactat caagcatctc caacctcttt aacggtaact gtctgtgcct
     7321 ccagttctgc tatgcgcttt tctgaggctt caagtaacgc ctgcttatcg cgtagcgctt
     7381 cttccagttc agcaacatgg cattcactat caataaggtt gttctctgct gcttccagct
     7441 caacacgcaa cttccctacc gttagcgcaa tttcctcgtt ctcctgatcg cggcttttga
     7501 tgtattgcag gtttctttcc cgttcatcca gtagtgccag cgcaacattt ggattaaaag
     7561 cagcaataaa ttcagcgttt gcataagcct gaa
//
LOCUS       DAJTYF010000157         7520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTYF010000157 DAJTYF010000000
VERSION     DAJTYF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.157"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..490
                     /locus_tag="OO590_003698"
     CDS             <1..490
                     /locus_tag="OO590_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6638894.1"
                     /translation="VSVATRQVAGNTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTA
                     RIRVNRGNLPAIKLNTTRRRAGEGLRVGKYFFRGAFVQQLANGRWHVLRCLPEARFAT
                     GHDHQGRLRKNRLPVEVVKIPMSGPLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHL
                     SK"
     gene            487..886
                     /locus_tag="OO590_003699"
                     /pseudo
     CDS             487..886
                     /locus_tag="OO590_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            897..1637
                     /locus_tag="OO590_003700"
     CDS             897..1637
                     /locus_tag="OO590_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6638895.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1696..2082
                     /locus_tag="OO590_003701"
     CDS             1696..2082
                     /locus_tag="OO590_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6638896.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2091..2420
                     /locus_tag="OO590_003702"
     CDS             2091..2420
                     /locus_tag="OO590_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6638897.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2392..5331
                     /locus_tag="OO590_003703"
                     /pseudo
     CDS             2392..5331
                     /locus_tag="OO590_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5331..5660
                     /locus_tag="OO590_003704"
     CDS             5331..5660
                     /locus_tag="OO590_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6638898.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5660..6358
                     /locus_tag="OO590_003705"
     CDS             5660..6358
                     /locus_tag="OO590_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6638899.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYDRFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6363..7106
                     /locus_tag="OO590_003706"
     CDS             6363..7106
                     /locus_tag="OO590_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6638900.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            7043..7520
                     /locus_tag="OO590_003707"
                     /pseudo
     CDS             7043..7520
                     /locus_tag="OO590_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
BASE COUNT         1774 a         1762 c         2423 g         1561 t
ORIGIN      
        1 ggtctcggtt gccacccggc aggttgccgg gaataccgtt gcgggcgata accaggtgaa
       61 agggatcccc ctgaaactgg tacgtcagcg tgtccgggtg tttaaagcca gtccgtcagg
      121 aaaaatgacg gccaggatcc gcgttaaccg gggcaatctg cccgccatca aactgaacac
      181 aacacggcgg cgtgctggtg aaggactgag agtgggaaaa tactttttcc ggggggcatt
      241 tgttcagcaa ctggcgaatg gccgctggca tgttctgagg tgtcttcctg aagcgcgttt
      301 tgcaacaggg catgaccatc agggcaggct aagaaaaaat cgtcttcctg tggaggtagt
      361 gaaaatcccg atgtccggac cgctgacaca ggcatttgaa gatgcccgcg atcgcatcat
      421 tgctgcggaa atgccgaaac agctggggta tgccctgaaa caacaactga ggttacatct
      481 gagtaaatga accggcacac acaaatccgt cagagcgtgc tggcacgcct tcgggaacag
      541 tgtggagaca gcgccacgtt ttttgacggg cttccggcat ttattgatgc gcaaactgcc
      601 tgccgtggcg gtgtggctga gtgatgctca gtacaccgga aaaatgacgg atggagatga
      661 ctggcaggct gttctgtata ttgccgtctt catccgggca caggcaccgg attcagagct
      721 ggatatgtgg atggagagca ccattttccc ggccctgaat gatgtaccgg cactttccgg
      781 actcatcgac accctgaacc cactcggttt taactatcaa cgtgataatg agatggctac
      841 ctgggcgatg gcggaaatca cgtaccagat cacgtacacg aattaaggag gtggtaatga
      901 ccacaccaaa tccactggca aaaacgaaag gtgcgggaac gacgttatgg atgtataccg
      961 gcaacggcga cgcatttgcg aaccctttgt cggacactga ctggctgcgt cttgcgatgg
     1021 tgaaggatct gcaacctggc gagatgaccg ctgatgcaga agatgacact tatctcgatg
     1081 atgaagatgc agactggaaa acgacaaccc aggggcagaa atccgtcggt gatacttcgg
     1141 cgacgctggc ctggcgtccg ggtgacagcg ggcagaaaaa actggttcag ttgttcgatt
     1201 ccggtgaagt ctgcgcgttt cgtatcaaat accccaacgg tacggttgat gttttccgtg
     1261 gctggctgag ttcactgggt aaaaccatta cctcaaaaga ggtgatgacc cgtactgtaa
     1321 aaatcagcgg tgtggggcgt ccgtatctgg cagaggaggg cgctgaaacc gtgggtgtta
     1381 ccggactgac ggtggcaccg gcatctgtca gtgtcaaagc gggagcaacc accacactga
     1441 cctttacagt aaaacctgac ggggccagtg acaaagcgat cagtgtgcat tcgtcagatc
     1501 ctcagactgc ttcggtgccc ctgagcgggc ttgtggccac ggtgaaaggc gtgcagcagg
     1561 gcagtgtcag catcgtgggc atgacctctg acggagagtt tgtggcagtg gctgcggtga
     1621 ccgtcagcgc accataacag gacgatactc atcattgccc cggttatccg gggctttttg
     1681 catccggaga acatgatgtt tctgaaacag gatacgttta attatgaaaa acagtccgtg
     1741 gtgctcagtg agctgtccgg gctgcagaga attgaatatc tgacgtttgt tcagcagcga
     1801 aaggcaaagt ttgatgcaca ggagggagaa ctgccggagg ctgaacgaca gattgctttt
     1861 ctgcgtatgg gaatggatat caatgcctgg ctggtttccc gctcactgtg gaatgctgag
     1921 cagtctcagg atgttgagac gctttgcgca tccattatga caacatggtc gtatgatgcg
     1981 ctgggcgcgg gggcggagag ggttctgtcg ctgagcggta tggggaccat tgagaatgcc
     2041 ggggatgatg atcatgaggc gctgacgccg gaaaagtcct gacgcgggaa atgcagtttg
     2101 tcatgcggct tgcccgggag ttccggcggg cagactggcg gcagatgctg tcggatatgt
     2161 cggccactga gcttggtgag tggggcgatt atttccggat gcagagcttc agtgatgtgt
     2221 ggatggatgc gcagtttgcc tcgctgaagg cattgatcgt gagaatggtg tccggcagca
     2281 gcgatgctgc ggtggctgat ttcagccttt taccggaaga gaacgggata ccggagcgaa
     2341 cggacgaaga actgatgcat cttggggaag gtatttccgg aggtgtgcgt tatggaccag
     2401 atagccaacc tggtcattga tttggggatt gatgcggcag agtttaaaaa tgaaattccc
     2461 cgtatcaaaa accttctgaa tggtgcagcc agcgatgcag aacggtcttc tgcccgtatg
     2521 cagcgtttta tggagcgtca gactcaggcg gcccggcaga caatgcaggc ggcttcttcg
     2581 gctgcaacag cagcatcagc ccatgcgcag acggtggaga agaatgcacg ggctcatgaa
     2641 cgcatggccc gtgaggtgga acaaacccgt ctgcgcgtgg atgccctgaa tcagaaaatg
     2701 cgcgaggaac aggcgcaggc cagggcactg gcggaggcgc aggataaagc ggctgccgcc
     2761 ttttatcgcc agattgacag tgtgaaacag gccggtgcgg ggcttcagga attacagcgt
     2821 attcagcagc agatccgaca ggccagaaac agtggcgggg ttggtcagca ggattatctg
     2881 gcgctgattt cggagatcac ggcgaaaacc cgtgccctga cgcaggcaga ggaacaggcc
     2941 acccggcaga aagcggcgtt tatccgccag cttaaagagc aggcaacccg ccagaatctg
     3001 tcgtcttctg agttgcttcg cgccagggcg gctcagctgg gggtgttaat cagtcagatg
     3061 gcgcgcggca attttggtgc gctgagggga tccgggataa cgctggctaa ccgtgccgga
     3121 tggacaggcg cactgatgtc gccgaaaggc atgatgactg gcggcgttat tggcggactt
     3181 gtcgcggcgg tcctgggtct gggtaaagcc tggcatgacg gcggaaggag ggcgaggaat
     3241 ttaaccgtca gctggcgctg acgggacatt atgccggtgt cactgtcggg cagttgtgga
     3301 aactcagccg ggccatatcc gggaatggta tcacgcaaca tgcggcagcc ggtgcgctgg
     3361 cacaggtggt gggcagtggg gcatttcatg gaaacgatat tggtatggtg gcgaaagctg
     3421 ccgcacagat ggagcgatcg gttggccagt cggtcagcga taccataaat cagtttaagc
     3481 ggctgaagga tgatcctgta aatgccgcga aggcgctgga caatgcgctg cattttttga
     3541 ctgccactca gcttgagcag atacgcgtcc ttggggagca ggggcggtcc agtgatgcgg
     3601 cccggatcgc catgtctgcg ctggcagagg aaaccggtaa acgcacatct gatattgata
     3661 ataatctcaa tgcgctgggt agtacgctgc aaaccttgtc tgactggtgg aagcagttct
     3721 gggatgcggc catgaatatt ggtcgtgaag actcgctgga tgcgcagatt gatgcgttac
     3781 aggaaaaaat tcagcgcgcg aaaaaatatc cgtggacaaa cgcctccaca caggtggagt
     3841 acgatcagca gcgtcttaac gatcttcagg agaaaaaacg ccggaaggat ttgcaggatg
     3901 caaaagcgca ggcagaacgg aattaccagg agcaacagaa acgccggaat gctgaaaatg
     3961 ccgcgctgaa ccggatgaat gaaacggaag ctgcacgaca tcagcgggaa attgcgcgta
     4021 ttaatgccat gcagtacgcc gaccaggctg tcagggatgc ggcgatacag cgtgaaaatg
     4081 aacgttacga aaaagccatt aagaaaaata cacgggcaac ccgtaatgat gaggccaccc
     4141 ggttattgct gcagtacagt cagcagcagg cacaggtgga agggcagatt gctgctgcca
     4201 gacagtcagc aggcattgcc actgaaagga tgactgaagc gcataaacag cttctggccc
     4261 tgcagcagcg catcagcgac ctggacggga aaaagctgac ggcagatgaa aagagtgtgc
     4321 tggcccgtaa gaatgagctg attcaggcgc tgacgctgct ggatgtgaaa cagcaggagc
     4381 tgcagaaaca gacagcgctt aacgacctga gaaaaaaaac ggttcagctg accagccagc
     4441 tggcagacaa agaacgtgca ctgcgtgagc agcacaatct ggatattgcc actgcaggta
     4501 tgggggataa gcagcggcag cgctaccagg cacagttgcg catccggcag gaataccggc
     4561 aacagttgca acagcttgag aatgacagtc gccagaaagg cacttacggg acggaggact
     4621 accggagggc tgaggaggtg ctgaagggga gcctgaagcg acaactgaat gaaaacaaac
     4681 gctactggca ggaactggaa gtggcgcagg gcgactggaa aaacggtgcc atgcgggcgt
     4741 ttcagaattt tacggcggat gcggataatg cggcgggaac ggcagaacag atgtttacag
     4801 tggcattcag cagtgccggt aatgcactgg cgacattctg taccaccggt aagctgaatt
     4861 ttaaatcctt cacctcttcc ctgttgtcag atatggccag aattatggca cagatggcca
     4921 tgatgcaggc ggtaaagggc gtcggttctt tattcggctt cacgactaat gctgatggcg
     4981 gtgtttacca gtctgctgat ttgagtcgct acagtggcac ggtggttaac cgtccgacgt
     5041 tttttgcttt tgcaaaaggc gcgggtgtga tgggggaagc gggacctgaa gccattctgc
     5101 cactgcgtcg cggtgctgat ggtaagctgg ggggttgtgg cggatactgg tggttcaggc
     5161 atggtgatgt ttgccccgca gtacaacatt gagatcaata acgacggcac gaacgggcag
     5221 ataggtccgg ctgccctgaa ggtggtttat gacctcggga aaaaagcggc cgcggacttt
     5281 atgcaacagc aggcccgtga tggtggccgg ttaagtggag cataccggta atggagacgt
     5341 ttcactggaa agtgcgcccg gatatgaatg tggtatcaga gccgaaagtg gtgacagtga
     5401 agctgggcga tggttatgaa cagcgtcgtg cggcgggact gaataaccag ttgtcgactt
     5461 acagcgtgac gatacgtgtt cgtaaaggtg aacacccatc tttaaaagcc tttctggaac
     5521 ggcacggtgg cgtccgcgca tttcagtgga cgccacctta tgactggaaa ctgatcaggg
     5581 aggtttgtcg taaatggtcg gcaagcgtgg gggcgttatg ggtgactgta acggccgatt
     5641 ttgaacaggt ggtgaactga tgcaggatat ccggcaggaa acactgaatg aatgcacccg
     5701 tgcggagcag tcagcccggg tggagctctg ggaaatcgat ctgacagagg tcggtggtga
     5761 gcgttatttt ttctgtaatg agcagaacga aaaaggtgag ccggtcacct ggcaggggcg
     5821 gcagtatcag gcatacccca ttcagggggc ggggtttgaa ctgaacggca ggggctgtgc
     5881 tgcccgtccg acactgacgg tctctaacct gcacggcatg gtcaccggga tggcggaaga
     5941 tctgcagagt ctggtcggcg gaacggtggt ccgccgtaag gtttacgacc gttttctgga
     6001 tgcggtgaac ttcgtcaacg gaaacagcga cgccgatccg gaacaggagg tgatcagccg
     6061 ctggcgcatc gagcagtgca gcgaactgag cgcggtcagt gcctcttttg tgctggccac
     6121 accaacggag acggatggcg cggttttccc ggggcgtatc atgctggcga atacctgtat
     6181 gtggacttac cgttctgatg agtgtggtta cacgggcagg gcagtggctg acgagttcga
     6241 caaaccaacg acggatatcc ggaaggacaa atgcagcaag tgtatgcgcg ggtgtgagtt
     6301 gcgcaacaat accggtaatt tcggcggttt cctttccatc aataaacttt ctcagtaaat
     6361 ccatgacaca gacagaatca gcgattctgg cgcacgcccg gcgatgtgcg ccagcggagt
     6421 cgtgtggatt cgtggtgaga acgccggagg gagacaggta tcttcccagc gagaatatct
     6481 ccggtgagcc ggaggaacgg ttccggatgg ctccggagga ctggctgcgg gcacaaatgc
     6541 agggtgagat tgtggcactg gtccacagtc atcccggtgg tctgccctgg ctgagtgagg
     6601 ctgaccggcg gctgcaggtg cagagtgatt tgccgtggtg gctggtctgc cggggggcga
     6661 ttcacaagtt ccgctgtgtg ccgcatctca ccgggcggcg ctttgagcac ggggtgacgg
     6721 actgttacac gctgttccgg gatgcttatc atctggcggg gattgagatg ccggattttc
     6781 accgcgggga tgactggtgg cgtcacggtc agaatctcta tctggataat ctggaggcca
     6841 cagggctgta tcaggtgccg ttgtcatcag cacaaccggg cgatgtgctg ctgtgctgtt
     6901 ttggttcatc ggtgccgaat catgccgcca tttactgcgg cgatggtgag ctgctgcacc
     6961 atattcctga acaactgagt aaacgagaga ggtataccga caaatggcag cgacgcacac
     7021 actccctctg gcgtcaccgg gaatggcacg catctgcctt tacggggatt tgcaacgatt
     7081 tggccgccgc atcgaccttc gtgtgaaaac gggggctgaa gccatccgcg cactggccac
     7141 acagctcccg gcgtttcgtc agaaactgag tgacggctgg tatcaggtgc gcattgccgg
     7201 gcgtgatgca ggtgaaaccg aattgtctgc ccgtcttaat gagccgctgg caaatggtgc
     7261 agtgatccac atagtaccgc gtctggtggg agctaaaagt ggcggtgtgt ttcaggcggt
     7321 gctgggggca gctgttatgg cggttgctat atggatgccg gggtaggaat tatggcgagt
     7381 aatctgctgt tttctctcgg tgccagtatg acgcttggcg gtgttgcaca gatgctggca
     7441 ccgaaagcca gaactccccg tacacagaca acggataacg gcaaacagaa cacctgtttc
     7501 tcctcactgg ataacatggt
//
LOCUS       DAJTYF010000158         7518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTYF010000158 DAJTYF010000000
VERSION     DAJTYF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.158"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            215..291
                     /locus_tag="OO590_003708"
     tRNA            215..291
                     /locus_tag="OO590_003708"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Arg,seq:tct)
     gene            complement(337..1098)
                     /gene="envY"
                     /locus_tag="OO590_003709"
     CDS             complement(337..1098)
                     /gene="envY"
                     /locus_tag="OO590_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6638901.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINYYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1281..2171)
                     /locus_tag="OO590_003710"
     CDS             complement(1281..2171)
                     /locus_tag="OO590_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6638902.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2172..5150)
                     /gene="nfrA"
                     /locus_tag="OO590_003711"
     CDS             complement(2172..5150)
                     /gene="nfrA"
                     /locus_tag="OO590_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCR6638903.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDP
                     ALIRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVG
                     RRWTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLS
                     VYSRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTML
                     RASASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANG
                     QTIEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFK
                     AINQRNGERNNAFLTIGVHW"
     gene            complement(5137..7374)
                     /gene="nrfB"
                     /locus_tag="OO590_003712"
     CDS             complement(5137..7374)
                     /gene="nrfB"
                     /locus_tag="OO590_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR6638904.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVISNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHGAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQAQAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
BASE COUNT         1753 a         1949 c         1963 g         1853 t
ORIGIN      
        1 tttccgttag ggcggtaaat tgtactttga tacatgaaaa tacgggtttt cttgattcag
       61 acgcgcagcg gtgtgcgttt gtttgccgct atagcgaaat aaatcagaaa atcagacgcg
      121 gtcgttcact tgttcagcaa ccagaccaaa agccattgac tcagcaaggg ttgaccgtat
      181 aattcacgcg attacaccgc attgcggtat caacgcgccc ttagctcagt tggatagagc
      241 aacgaccttc taagtcgtgg gccgcaggtt cgaatcctgc agggcgcgcc atttcttctt
      301 catttatgcc cgtcttatcc gtttccgctt tgcccttcac cacatcactt tttgtcgctg
      361 tttggcgaga taattcaacg gtgtcaggct gtaaaacgcc ttaaaaacag agataaagta
      421 cgacgtgctg ctatagccac ataattgcgc cacctgagtg atatttttgt tatccatcaa
      481 taacatctgt acggcgtaac gcatacgaca ctctgtgaca atctggctat agctggtatt
      541 ttcgtttttt aatttctttt tgagcaggct ggggcttaaa catagcgaac tggcgacaat
      601 tcgcagattc cagtaatgct gaatatcgct ttgaataatg cggcagacgc tgtcgcggac
      661 gctgctgcgt aagatataca tcagtagggc aataaaccgc gattgctcaa gaaagttaga
      721 caatacggta aaaagtaatg cgcgcgtcaa ctccgcttcg cagggtttgc tgggttgctg
      781 cttgctgtgg ttggcggcta gccggaacac ttccggtgta cggctatgac aagcgataac
      841 cggggttgcc agccgcgacc agggctttac acaggttaat ttcttattta aaaacaacag
      901 ataatagttg atgatgttac ggttgatgtg ggtgattaat acgtgattta acgttgagag
      961 ttcaatgacg ttattgttgc acgcgaaggc ggccaggtag tttttcggaa gggtaaacgt
     1021 agcatggtta ttgacgctta cctctacctc tttttcggtc aggatcacca cgcaaggttc
     1081 actgctgctc aattgcattt cgcactcctc agatatcaga aactccgctc aaaggatcta
     1141 tgcttcctgc atgagtgatc ggcccgttcg ccgataacga tcttctttct ttagcatgct
     1201 ttttagcaat taatcttgat ggaattctga cgagagcgaa agaggtaagc caggtcgtac
     1261 ccgacttacc tggaggggat ttaatactcg agaatgccgt gcgcgacggg caaatagcgc
     1321 agagagaaat agagcatgtc tttaccgcaa gaggaacgtt tcgctaacag cgaggcaatt
     1381 tctgcgtccg gtttcggttt cgctgtaaag gttttgcctt tgccggcgac aaacagttca
     1441 taaacaatgc cgcttgctgg ggcggagctt tggcaatcgg cgctggcctg tagataacgt
     1501 tcacgctgtt cagcggtaag tttatccacg ccgctgccat cctgtaccca gacattaact
     1561 ccggttgctt taacttgttc cagcagttgg cgatagccat cgggcgacat gtttccggcg
     1621 aaaaaactac tgatataaac cggttttgct gaaacatcgc taatcagccg ctgcgcgttg
     1681 tttaaccatg ttagcaaagg ttgacgggcg gcttcgctgc gccagttcag gtcgtcaatt
     1741 tccgcgctga tgtaccagcc atccggcgtt atgccaggcg cggcgctcca taatctggct
     1801 tgctggagat cggcagccag caggcgatta agatagcttt ccagcgctgc agacgactgt
     1861 ttctggtgca taaaaaattc cggatcggcg ttcagcccga caatcagctt caggccagtc
     1921 tgttgcgcag ctgcggcacg cttaaacaat aacgtgcgct gttctggctg ggtaaatgca
     1981 tcgccgtaac gggtccattg caaaaccagg gtatcgaagc cttgaaaacg taactgactc
     2041 atcagcccct gccactgggt atcggtaacc tgactatctc ggttttgtgg ttgccagata
     2101 atacctttca tcgcaaagga aaaagggctg accaaaagca gtgtcagcaa tacgaaaatg
     2161 aacttacgca tttaccagtg cactccaatg gtgagaaacg cgttgttgcg ctctccgtta
     2221 cgttgattaa tcgccttaaa ggtatgttga tactcgacgc cgagactgac tttgtgcggc
     2281 caggcgtcgt agtgcgtctc gccggtccaa atattccagc ggaccccgac accgccaagc
     2341 tgcgcgccct gagtgccttt atcacgatag ccgttgtcct gaacgtgagc gtaaggctca
     2401 atagtctgtc cgttagctac cttctgatgc cagctgacgc gataatctgc cgtccacgcc
     2461 tgaatatcct ggcggatata ttgcgccgca tcgaggtaca ggttttgggc aaaccagcct
     2521 gaaccgttcg ggtgccattc gtcgctgtat ttgccgccat taaagaatga ggcgctggcg
     2581 cgcagcatgg tatcggatgc gccattttgc ccgttcagcg gcaactgctg ttcgacggcg
     2641 aggaaaaaga tctgatcgcg cagcggcttc cagcgcagac cggtgccgga catcggattt
     2701 ttcaccggca tcatcacccc gttttctccg gtatcggcaa agacgcggct ataaactgag
     2761 agcaggtcgc cttccagcag catattgcgt ccgatgcggt actcggcttc cagttgtccg
     2821 tagctacgat agcttttccc aggcgctgca ccgccgacat tattgttagc ggtactcatt
     2881 gcgccggaac gcaagccgat ggaagaatcg aaactgaacg tccagcggcg accgacctcc
     2941 tcatgcaaac ggcggaaatt gaagcgttgc tgattttgct ctggggtcag tggggttatc
     3001 agcgcctgat tatcaatgtc atcaatcacc agccgggcgt agtgctgcgt cgcaggcatg
     3061 tcatccagac gctggttcac gtaggccagt tgtcggatca gtgccggatc gtccggaagc
     3121 cctttatgcg cctgttcgag catttcccgc gactgtgcga tatcaccgct atcccacaag
     3181 gcgtaaccaa gcgctgcctg ggtgttgcta ttattcggtt ccagttccag ttccagcgcg
     3241 gcgcgcaaat cactcaccgc ggccggaaca ttatgacgtt ggcgataaat tgtcgcccgc
     3301 gcaacgtaag cgttggcaga aggggcaata ttgattgagc gcgtgagatc gttcagtgcg
     3361 agttctggct gagctgggat ataacgttgc gcatgcagcc accagtattg agcattgttt
     3421 cccagctcac gttgttctgc ctgttgaagc cagcgatcgc gagccgcgcc atttcccgct
     3481 gcctgagcgg tgttggcggc ggcgagcaga tcctcattgc tcatgtcgtg aagactgatt
     3541 ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg ataggctacc
     3601 gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc atacaacgcc
     3661 acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc agcatcgtag
     3721 gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc aatacccggc
     3781 aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa aatcgccacc
     3841 ttcgccggcg ttgctaggta aggatgactt tccagcagag acgccagtcg cgccattaaa
     3901 gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa atcggcagct
     3961 tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg gggaagattt
     4021 gtcggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt gttacgggtc
     4081 gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt ggcgagcagt
     4141 ttttgcgctg cgtcgtactg accctctttt aacagcaccg gtagcgtcgc gccaacaaca
     4201 tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac ggggttggcg
     4261 ctgtatttag ataacagata gagccaactt ttctcttgtg cgcccgtggt aaacagcggc
     4321 ttattttcaa tgagataatg ctggaggcgt gctttttcgc cacgataagc cagcgcggtc
     4381 gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag tgccaggatc
     4441 cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc tgccgcgctt
     4501 aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca ttgtttcagg
     4561 tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga tgcagcaaac
     4621 gtcgcatcgt tcagttgcgc tctggcgaca ggtaattgtg ccagccgcag ggcattctgc
     4681 ccgacttcac tgcgacaacg cagggtcggc gcagcgtcgc ccgctttttg ctgggcaagc
     4741 agttcttcaa cagtcgtaac gcttttcact tcaactggaa tagccgccag actgcgctca
     4801 agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg cgcccggtca
     4861 tcatgaccaa aatgacgata ggcttccgca aggtataaag tcagcggaat attatccggc
     4921 acctgttggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt atttttctgt
     4981 gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc gctcagcccc
     5041 agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa taaagacgtc
     5101 agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat tttcggactc
     5161 cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt gcatcgaaac
     5221 ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga taacgccttc
     5281 ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata acaacacgtt
     5341 aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca ggaactgatg
     5401 cggcacatat tgccgccaga tttcaccggt ctgctgttcc gtgagccact gatgctgaac
     5461 cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc agtggcgtaa
     5521 ccctgtgaca atttgtcccc gcagaacaat gacgtaacgc actttgcgtc cgactttacg
     5581 cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa ttaactcatc
     5641 attttccaga cgcagcggca gtactgcata atgcagcgct acggaggctg gcatttcggc
     5701 aatcagcgag gacgggatct gccaggcatc gatggattcc cacgccacgc cgttttgctc
     5761 tgcctgcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca ttgaaccgcc
     5821 caggcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttctt cagtgatgac
     5881 ctgattttcc agcagaattt gacctaacgg gcgcaacgag cgggtatcgc cagtgacgct
     5941 ggggaagtca tgcgttgttt tatcccacgc cacgcgacgc ggatcgccgt gctgaagaac
     6001 ctgtttcagc acgcgccagt tagccataaa gttaatcagg ttgccccaga aaagacgcag
     6061 gacggaaagc aggccctgcg tgaggccgta gtagccagtg acgaaaatca ctcgctgcac
     6121 gatgcggtta accatcaagc caaagtttag ccacagcagg gtcattaacc atgcgctgcc
     6181 gctaaaaata gaaaggaaat gccaggcatc gggccacaaa ctttcatacg ccagcaacag
     6241 caaaagctgg atcatcacca gcatcgcgag gaagctgaca aagttactga ttgccccttt
     6301 gcggtcgcgc cagagaaagt agttcagcgt caggctggag gtccatttat gggttttaaa
     6361 gccctggaaa acaatgccga tgatccagcg agatttttgt cgaaccgcag tcgaaaaggt
     6421 atcggggaaa tattcgcgca cgcagatcat gtttgacgtc cgtgcgtgct gtaaaaattt
     6481 acgctgctcg cgttctttgg cttcgtccac caccggaaaa cggacaaaaa tttccgtcat
     6541 acctttttct ttcaggcgga agccaatgtc gtaatcttca gtaagactct gcacgtcgaa
     6601 agcaataccg ttaccgtcag ctaacagtgc ggtcacggcg cggcggctga aacaggtgcc
     6661 gacgcctgcg ctgggcactt gtccggcgag ggcttcacgc accggaacat ctttgccatg
     6721 cagttctgaa aactcatcaa tgtaagtcat gctggtgaag tgcgtccatt cgcgttcaaa
     6781 cggatacact gggatctgaa tcagatcttt acgctcgacc agatagttga acagacgcaa
     6841 ttccatcggt gaaatcacat cttcggcgcc atgcagaata aaaccagcaa aagcgaaatt
     6901 ggcgctacgt tcaaactggg tgatggcgtc cagcacgttg ttcagacagt cggctttgct
     6961 ggtgggacca ggacgcgcgc agactacctt atgcacgttc ggaaagcgag cgcacacttc
     7021 gtcaacatca cgttgagtat cggggtcgtt ggggtaggtg ccaacaaaga tatgatagtt
     7081 ttcgtagtcg agcgtggtcg ccgccagctc ggccatattg ctgatgacgc ccgtttcatt
     7141 ccacgccgga accataatcg ctaacggttt ttcatctggt ttatacagtt cgcggtaact
     7201 cattcgcggg tagcggcgat aaacactcaa cttgcgttta atgcggcgta cccagtagac
     7261 gacatcaata aaaaaatcgt ccagcccgct gatgaacatg atgaccgcta acgttatcgc
     7321 gattactttt aagccgtaga gccaggtagc aaaaacatca agaagccagt ccacacaaaa
     7381 accttacatt aacgctggtt atgtttaggg tggcgtatat taaggttttt tatgaattgt
     7441 gacagctttt taccattaat agggtaatga ctccaactta ctgatagtgt tttatgttca
     7501 gataatgccc gatgacct
//
LOCUS       DAJTYF010000159         7498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTYF010000159 DAJTYF010000000
VERSION     DAJTYF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.159"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..1143
                     /gene="rbsA"
                     /locus_tag="OO590_003713"
     CDS             <1..1143
                     /gene="rbsA"
                     /locus_tag="OO590_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCR6638905.1"
                     /translation="ADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKVIIM
                     DEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFIAER
                     EVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKGEIL
                     GVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDDLANGIVYISEDRKRD
                     GLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIGLLS
                     GGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVSSEM
                     PEVLGMSDRIIVMHEGHLCGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            1148..2107
                     /gene="rbsC"
                     /locus_tag="OO590_003714"
     CDS             1148..2107
                     /gene="rbsC"
                     /locus_tag="OO590_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6638906.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIVGT
                     LIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2132..3009
                     /gene="rbsB"
                     /locus_tag="OO590_003715"
                     /pseudo
     CDS             2132..3009
                     /gene="rbsB"
                     /locus_tag="OO590_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3135..4064
                     /gene="rbsK"
                     /locus_tag="OO590_003716"
     CDS             3135..4064
                     /gene="rbsK"
                     /locus_tag="OO590_003716"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6638907.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQLLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4068..5060
                     /gene="rbsR"
                     /locus_tag="OO590_003717"
     CDS             4068..5060
                     /gene="rbsR"
                     /locus_tag="OO590_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6638908.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5026..6453)
                     /gene="mdtD"
                     /locus_tag="OO590_003718"
     CDS             complement(5026..6453)
                     /gene="mdtD"
                     /locus_tag="OO590_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6638909.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVTRLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6476..7168)
                     /locus_tag="OO590_003719"
     CDS             complement(6476..7168)
                     /locus_tag="OO590_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6638910.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1906 a         1815 c         1976 g         1801 t
ORIGIN      
        1 gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt agtgggcgat
       61 ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt tgagtcgaaa
      121 gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga atccctgttc
      181 cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc ccaccgcatg
      241 aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca atttattgct
      301 gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt gggccgcaag
      361 ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct gaaagtcgat
      421 aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg cgaaattctt
      481 ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct ctacggcgca
      541 ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac ccgttcaccg
      601 caggatgacc tggcaaacgg cattgtgtat atctccgaag accgtaaacg tgacggttta
      661 gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta cttcagccgc
      721 gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt cattcgtctg
      781 tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg tggcaatcag
      841 caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat ccttgatgag
      901 cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat taaccagttc
      961 aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt attaggcatg
     1021 agcgatcgca tcatcgtcat gcatgaaggg catctctgcg gggaatttac tcgtgagcag
     1081 gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt gaatcaggag
     1141 taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag cgtggctgat
     1201 ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga cgttaagccc
     1261 gaactttttc accatcaata acttattcaa tattctccag caaacttcgg tgaacgccat
     1321 tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt cggtaggttc
     1381 tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag tcaatgcgct
     1441 ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa ccggggtgat
     1501 tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc ttttactgcg
     1561 cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta ctgagaacgc
     1621 cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc cagtctggat
     1681 catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc tggggcgtta
     1741 catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca acgtcaataa
     1801 aatcatcgtc tattctcttt gtggtctgct ggcatcgctg gccgggatca ttgaagtggc
     1861 gcgtctctcc tctgcacaac ccacggcggg gactggctat gagctggatg ctattgctgc
     1921 ggtggttctg ggcggtacga gtctggcggg cggaaaaggt cgcattgttg ggacgttgat
     1981 cggcgcatta attcttggct tccttaataa tggattgaat ttgttaggtg tttcctccta
     2041 ttaccagatg atcgtcaaag cggtggtgat tttgctggcg gtgctggtag acaacaaaaa
     2101 gcagtaataa cgactacagg acatcttgaa tatgaacatg aaaaaactgg ctaccctggt
     2161 ttccgctgtt gcgctaagcg ccaccgtcag tgcgaatgcg atggcaaaag acaccatcgc
     2221 gctggtggtc tccacgctta acaacccgtt ctttgtatcg ctgaaagatg gcgcgcagaa
     2281 agaggcggat aaacttggct ataacctggt ggtgctggac tcccagaaca acccggcgaa
     2341 agagctggcg aacgtgcagg acttaaccgt tcgcggcacc aaaattctgc tgattaaccc
     2401 gaccgactcc gacgcagtgg gtaatgctgt gaagatggct aaccaggcga acattccggt
     2461 tatcactctt gaccgccagg caacgaaagg tgaagtggtg agccacattg catctgataa
     2521 cgtactgggc ggcaaaatcg ccggtgatta catcgcgaag aaagcgggtg aaggtgccaa
     2581 agttatcgag ctgcaaggca ttgccggtac atccgcagcc cgtgaacgtg gcgaaggctt
     2641 ccagcaggcc gttgctgctc acaagtttaa tgttcttgcc agccagcaga ttttgatcgc
     2701 accaaaggtt tgaacgtaat gcagaacctg ttgaccgctc atccggatgt tcaggctgta
     2761 ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcaaa ctgccggtaa atcggatgtg
     2821 atggtcgtcg gatttgacgg tacaccggat agcgaaaaag cggtgaatga tggcaaacta
     2881 gcagcgacta tcgctcagct acccgatcag attggcgcga aaggcgttga aaccgcagat
     2941 aaagtgctga aaggcgagaa agttcaggct aagtatccgg ttgatctgaa actggttgtt
     3001 aagcagtagt tttaatcagg ttgtatgacc tgatggtgac ataaatacgt catcgacaga
     3061 tgaacgtgta atataaagaa aagcagggca cgcgccaccc taacacggtg gcgcatttta
     3121 tggacatccc gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc attaatgctg
     3181 accacattct taatcttcaa tcttttccta ctccaggcga aaccgtaacc ggtaaccact
     3241 atcaggttgc atttggcggc aaaggcgcga atcaggctgt ggctgctggg cgtagcggtg
     3301 cgaatatcgc gtttattgcc tgtactggcg atgacagcat tggcgagagc gttcgccagc
     3361 tgctcgccac tgataacatc gatattactc cggtcagcgt gatcaaaggc gaatcaacag
     3421 gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt catcggtatt catgccggcg
     3481 ctaatgctgc tctttcccca tcgctggtgg aagcgcaacg tgagcgtatt gccaacgcgt
     3541 cagcgttatt aatgcagctg gaatcaccac tcgaaagtgt gatggcagcg gcgaaaatcg
     3601 cccatcaaaa taagactatc gttgcgctta acccggctcc ggctcgcgaa cttcctgacg
     3661 aactgctggc gctggtggac attattacgc caaacgaaac ggaagcagaa aagctcaccg
     3721 gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg catgaaaagg
     3781 gtatccgtac tgtactgatt actttaggaa gtcgtggtgt atgggctagc gtgaatggtg
     3841 aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga taccattgct gccggagata
     3901 cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa accattgcca gaggcgattc
     3961 ggtttgccca tgctgccgct gcgattgccg taacacgtaa aggcgcacaa ccttccgtac
     4021 cgtggcgtga agagatcgac gcatttttag acaggcagag gtgacgcttg gctacaatga
     4081 aagatgttgc ccgtttggcg ggcgtttcta cctcaacagt ttctcacgtt atcaataaag
     4141 atcgcttcgt cagtgaagcg attaccgcca aagttgaagc ggcgattaaa gaacttaatt
     4201 acgcgccatc agctctggcg cgtagcctca aactcaatca aacacatacc attggcatgt
     4261 tgatcactgc cagtaccaat cctttctatt cagaactggt gcgtggcgtt gaacgcagct
     4321 gcttcgaacg cggttatagt ctcgtccttt gcaataccga aggcgatgaa cagcggatga
     4381 atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg cttactgtta ctgtgtaccg
     4441 aaacgcatca gccttcgcgt gaaatcatgc aacgttatcc gacagtgcct accgtgatga
     4501 tggactgggc tccgttcgat ggcgacagcg atcttattca ggataactcg ttgctgggcg
     4561 gagacttagc aacgcaatat ctgatcgata aaggtcatac ccgtatcgcc tgtattaccg
     4621 gtccgctgga taaaactccg gcgcgcctgc ggttggaagg ttatcgggcg gcgatgaaac
     4681 gtgcgggtct caacattcct gatggctatg aagtcactgg tgattttgaa tttaacggcg
     4741 ggtttgacgc catgcgccaa ctgttatcac atccgctgcg tcctcaggcc gtctttaccg
     4801 gaaatgacgc tatggctgtt ggcgtttacc aggcgttata tcaggcagag ttacaggttc
     4861 cgcaggatat cgcggtgatt ggctatgacg atatcgaact ggcaagcttt atgacgccac
     4921 cattaaccac tatccaccaa ccgaaagatg aactggggga gctggcgatt gatgtactca
     4981 tccatcggat aacccagccg acccttcagc aacaacgatt acaacttact ccgattctga
     5041 tggaacgcgg ttcggcatag atttacgctg tcttttgatc aaattattac catcggttgt
     5101 tttcagaagc atgaacattg ctgctgaagc aacagtaata atgcccatcg tgataaacgt
     5161 atagtggaat tgttcgacag tcgttgtgcc ttccatccct tcataaatgc gaaggacggc
     5221 cgcacttaca gcaacgccta aactaatcga cagttgctgc gtgaccgcca gaacattgtt
     5281 accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg atggtattca tcgcggtaaa
     5341 ctgcgtcgac atagccatcc ctaatataaa caacggcaag atcagcatcc atatcgccat
     5401 tgccggtgat tgcaaagaga actgagcgat cattagccca ataatcaccg tgatccccac
     5461 taatgtatgg cgatagccca gacgacgtaa gacttgggta accatcgatt ttgcaataat
     5521 ggaacctaac gctgtcggtg ccatcataca gccggcaata aacgcctgat aaccaaatcc
     5581 tacctgtaac atcaatggca taaggaacgg tacacagccg gtccccagac gggttgcaat
     5641 attgcctacg ataccgatcg agaaagtgcg ggttttaaat aaatctaatg aaattaatgg
     5701 gtttggcgta cgtcgcgcat ggagaatata gagaagcagt aacccgatgc tggtgacaat
     5761 taccgtcaag gcaatccagc tggcgacaat cttttccccg aatagctcta ttccgcttga
     5821 aaagagaaca aggctgaggc caaacagcaa aaagccagtg atatcgaatc tgcgtcgtgc
     5881 ggtggtgaaa ttgggcatat gtttgcgcgc gtaaagaagg cccgcaatac ctatggggat
     5941 attaattaaa aatatccagt gccaggttgc ccaggtgacc agcacgccgc caagaacggg
     6001 gcctaaaatt ggccccacca gacccggcat ggcgacaaaa ttcaataccg gaagaagttc
     6061 attacgagga taagcacgca gtaaggccag ccgagtaaca ggcatcatca ttgcgtcgcc
     6121 tatcccttga ataacccgga agacaaccag ctgtggtagc gaattagaaa gtgcgcaggc
     6181 cagagaaccc aacgtgaaca gactcacggc aagggtaaaa atgcgacgcg taccgaagcg
     6241 atcggctagc catccgctta ccggaataag catcgccacc gtcagcgtat aactgatgat
     6301 ggctgattgc atcgcgagag gagaacgatt aaggctatga gcgattgcgg gtaaggcggt
     6361 attcagaata gtggcatcaa gtgcctgcat gaaaaaggcc atcgccgcga tccacggcaa
     6421 acccgacata ctacgcttct ttttatcgct cattcaatgt cctgttatcg ggttatcact
     6481 tatcaggtga gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca tcgctttgga
     6541 taatcgcatc gacaatcgcc tggtgcagat ccagctttat cactgtgtcg ctggtaattg
     6601 acgtgaagta agtgtgataa accgaatgga atagcgaggc gaatgaggtc aaaaacggat
     6661 tggcgctcat ttcatagata tgctcatgcc aggctatatc gacttcgatc cagcgttcac
     6721 ggcgaaagtt ctcttttaat gccgccattt cggccattaa cgtattgaga tgcgccttct
     6781 gttccgcggt gccaaccgtt gctgccagta ggcaggcttg cggctccaga cagatacgca
     6841 taaccagaaa gtgatcgatg acctgatgaa agttctcttc tgtcatccac caagtaagca
     6901 attcctgatc aagaaaattc cagttcgatt gtggcatgac ccgagtacca attcgcggtc
     6961 gcggtaaaac catccctttt gccgttaacg ttttgaccgc ttcgcgtacc gctgtacgac
     7021 tcactccaaa ttgctcgccc agctcaatct caccgggcaa aatggtgccg ggttcatatt
     7081 caccttttaa gatccgttgc gccagcttct cagccagaac atacgaaagg tttttctgtg
     7141 cagctaactg ttgtgcgctt aaaggcatta cttatcttcc tttttctttt tattcctcct
     7201 tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg gcaaattgat tgttttatgg
     7261 cacattaacg gggcttttgc tgaaaaaatg cgcggtcaga aaattatttt aaatttcctc
     7321 ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca acggcaaaca
     7381 cgccgccggg tcagcgggat tctcctgaga attccgtcag agaaagcaaa aataaatgct
     7441 tgactctgta gcgggaaagc gtattatgca cacctcgcgc cgctgagaaa aagcgaag
//
LOCUS       DAJTYF010000160         7464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTYF010000160 DAJTYF010000000
VERSION     DAJTYF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.160"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            18..1076
                     /locus_tag="OO590_003720"
     CDS             18..1076
                     /locus_tag="OO590_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6638911.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="OO590_003721"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="OO590_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6638912.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVIHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /locus_tag="OO590_003722"
     CDS             complement(1623..1727)
                     /locus_tag="OO590_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638913.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="OO590_003723"
     CDS             1897..2343
                     /locus_tag="OO590_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6638914.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3121)
                     /gene="nimR"
                     /locus_tag="OO590_003724"
     CDS             complement(2300..3121)
                     /gene="nimR"
                     /locus_tag="OO590_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR6638915.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3218..4399
                     /gene="nimT"
                     /locus_tag="OO590_003725"
                     /pseudo
     CDS             3218..4399
                     /gene="nimT"
                     /locus_tag="OO590_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4454..4801
                     /locus_tag="OO590_003726"
     CDS             4454..4801
                     /locus_tag="OO590_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6638916.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4823..5077)
                     /gene="yoaF"
                     /locus_tag="OO590_003727"
     CDS             complement(4823..5077)
                     /gene="yoaF"
                     /locus_tag="OO590_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6638917.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5260..6285
                     /gene="dgcP"
                     /locus_tag="OO590_003728"
     CDS             5260..6285
                     /gene="dgcP"
                     /locus_tag="OO590_003728"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6638918.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6318..6416
                     /locus_tag="OO590_003729"
     CDS             6318..6416
                     /locus_tag="OO590_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6638919.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6419..6493
                     /gene="yoaJ"
                     /locus_tag="OO590_003730"
     CDS             6419..6493
                     /gene="yoaJ"
                     /locus_tag="OO590_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR6638920.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6545..6976)
                     /locus_tag="OO590_003731"
     CDS             complement(6545..6976)
                     /locus_tag="OO590_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638921.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKALEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(6970..7197)
                     /locus_tag="OO590_003732"
     CDS             complement(6970..7197)
                     /locus_tag="OO590_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6638922.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         1832 a         1792 c         1802 g         2038 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tagccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttaccgggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gtggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacaa cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttatccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggc accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcgtaaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccctag gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc agtcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caatgacccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatt aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 ttgtcggcat tgtcactgcg ctgggttcga taaacagaaa acagagttca gcgttagctg
     2881 tcacttgatt gctatgcttc accccgcccg gtatccaaac cgcatattgt ggcggcacca
     2941 tccacaaagc attttccacc gtacaggtaa ttgcaccatg taatgctagt atcagctgtc
     3001 ctttgcggtg ctgatgtaca ggactggtta actcatccgt tccggcgtga atacaaaatg
     3061 cgaccgcggc ttcatggtgt cggtcgggtt catagccatt gagattcaac ctgtgcatca
     3121 ttttgtccga acttagcgat aatttgtcat tttagcttga ttcaacataa caataaaaac
     3181 ggtaaggtac agcctcgttt gtaacaatga gaagcatatg acctgttcaa cttcattaag
     3241 cggcaaaaac aggattgtcc ttatcgctgg cattctgatg attgccacaa cattacgcgt
     3301 cacctttacc ggcgcagcgc cgttactgga tacgattcgt tccgcttact cgctgacgac
     3361 agcgcaaacc ggcttattaa ccaccctgcc attattggcc tttgcgctaa tctcacctct
     3421 ggctgccccg gtggcgcgac gttttggtat ggaacgtagc ctgtttgccg cgttactttt
     3481 gatctgtgca ggtatcgcaa ttcgttctct cccttcgcct tacttattat ttggcggtac
     3541 agcggtcatt ggcggtggga ttgcattagg caatgtctta ctgccaggat taattaaacg
     3601 cgatttccct cattccgtcg ccagacttac cggcgcatat tccctgacaa tgggagctgc
     3661 agcggcactg ggatcggcta tggtcgtgcc gctggctttg aacggttttg gctggcaagg
     3721 cgcgttgttc atgctgatgt gttttcctct gctggctctt tttttatggc tgccacagtg
     3781 gcgaagtcag caacatgcaa atttgagtac ctcacgcgcc ttacatactc ggggtatctg
     3841 gcgttcgcca cttgcctagc aggtaacatt gtttcttggg atcaactcac tggtctatta
     3901 cgtgattatt ggctggcttc cggcgatcct catcagtcac ggctatagcg aagcacaggc
     3961 gggttcactg catggtttgc tgcaactagc cacagcagct cccggtttgc tgatcccact
     4021 tttcttacat catgtgaaag accagcgtgg tattgcagcg ttcgttgctt tgatgtgcgc
     4081 agtgggcgcg gttgggctct gctttatgcc agcgcacgcg atcacctgga ctctgctttt
     4141 cggttttggt tccggcgcaa caatgatact ggggttgacg ttcattggtc ttcgggcaag
     4201 ttctgcgcat caggcggcgg cactctcggg gatggcacaa tccgtcgggt atttgctggc
     4261 agcctgtggg ccgccgctaa tgggtaaaat acacgatgct aacggtaact ggtccgtacc
     4321 actgatgggt gttgcaatac tttcactact gatggcgatt ttcggacttt gcgccgggag
     4381 agacaaagaa attcgctaat atccggtgct atagtgacgt gacaaatctt gcgtgaaagg
     4441 gagaacaaac acgatgaata ttcagtgcaa acgcgtttat gatccggctg aacagagcga
     4501 tggttatcgc gtactggtcg accgcctctg gccgcgcggt atcaaaaaaa ccgatttagc
     4561 ccttgatgag tgggataaag aaatcacgcc gtcaacggaa ctgcgcaaag cctttcacgg
     4621 cgaagtcgtc gattttgcaa cctttcgcga gcaatatcgc gcagaactgg cgcaacacga
     4681 gcaagaagga aagcggctgg cggacatcgc caaaaaacag ccgctgaccc tgctctacgc
     4741 agcaaaaaac accacgcaga accatgcgct ggtgctggcc gactggctac gtagcttgtg
     4801 attttagtac ggcatccggc ggttattttt caccagccgg atggtcccgt cgccacaatg
     4861 cccattcatc aatcgtttca ccgcccggta atttgcaatt gttgctgacc ccttgcgctg
     4921 tctgcactgg aatgagcgtc ccgcccttct gctggcaata gaccgacgcc ggatttgcca
     4981 taccaatctg cggcggttta ggtgcttctg gctgagaagg ggttgaacaa ccagccagga
     5041 ccagcaagca aggcagaaca aaactgataa ttttcattta ttgatctcac atatttatcc
     5101 aagcttagag tatcgcggta tcgttttgtt ttgcagcact atttttatta cattcactca
     5161 aaacatatta cgtcttgttt catctttgtt gatgatgttt tatcatgcct gcaaagatta
     5221 aataatcagc acttacccgc cgtatcctgg agttgttccg tgtcagatca gattatcgcc
     5281 cgcgtctcgc aatcccttgc caaagaacag tcactggaaa gtctggtccg acagcttctg
     5341 gagatgctgg aaatggtcac tgatatggaa tcaacctacc tgaccaaagt ggatgtcgaa
     5401 gcgcgcctgc aacatattat gtttgcccgt aacagccaga aaatgcacat cccggagaat
     5461 tttaccgtct cgtgggatta ctcgttatgc aaacgcgcca ttgatgaaaa ctgctttttc
     5521 agcgatgaag tccctgaccg ttggggcgac tgtattgcgg cacgcaatct tggcatcacc
     5581 acatttctga gcacgccaat tcacttaccg gatggatcat tctatggcac gctttgcgcc
     5641 gccagcagtg agaagcgcca gtggagtgaa cgcgcggaac aggttttaca gttattcgcc
     5701 ggactgattg cacaatatat tcaaaaagag gcactggttg aacagctgca cgaagccaat
     5761 gctgcgctga ttgcgcaatc gtataccgac tcgttaaccg ggctaccgaa tcggcgggcg
     5821 atttttgaaa atctgacgac gctgttttcc ctcgcccggc atcttaacca taagataatg
     5881 atcgcgttta tcgatctgga taacttcaaa ttaatcaatg atcgttttgg tcataatagt
     5941 ggcgatctgt ttctcattca ggttggcgag cgccttaata cgctccagca aaatggcgaa
     6001 gttattggtc gtctcggtgg tgatgagttt ttggttgttt cactaaacaa cgagaatgcg
     6061 gatatttcgt cgctgcgaga acgtattcaa cagcaaatac gtggagaata tcacttaggt
     6121 gatgttgatt tgtattatcc cggtgccagt cttggcatag tagaagtcga tcctgaaaca
     6181 accgatgcag acagtgccct gcatgctgcc gatattgcga tgtatcagga gaaaaaacac
     6241 aaacagaaaa caccttttgt cgcgcatcca gcgctacatt cctgaggcgt attcacatcc
     6301 ttttgattgg tgataacatg cgaatcggta ttatttttcc ggttgtaatc ttcattacag
     6361 cggtcgtatt tttagcatgg ttttttattg gcggctatgc tgccccggga gcataaagat
     6421 gaaaaaaaca acgattatta tgatgggtgt ggcgattatt gtcgtactcg gcactgagct
     6481 gggatggtgg taacgtcacc tctaaaaaat agcaaaggct gcctgtgtgc agcctttgtg
     6541 caatttaagc gttaactttt aatcttcctg tagataaata gcacgacaat cgcaccaata
     6601 acggctgaag catttcgtct gcagtctgaa ccgcaggcaa ccatttttca gcaagtctgc
     6661 gctgggaatc gctggcttta tcccatttgc tccacagtgc ctcacgatcg taatcgtgcg
     6721 cttccagcgt ggggcgggcg atttcaaaat acccctggct tgtttctatt tgctcagatt
     6781 ttaataacaa ctcaacacgt gctgccgctg gtagaactga tatatgaaat tccaaagcct
     6841 ttgaacccgt acgcttgcga cgtagttctg gatgattggc tgcaagcttt tcaagattgc
     6901 ggcgaatact cacctcatgt ttaggaaagc atgacattcc tacgcattct tgcgctgtta
     6961 cccaatgggt taacatgatg ttttctccat ataccgagaa ggccaaatct gggctggttc
     7021 catatccaaa tatctagcaa tgattgcttc gtatcgtggt atatggcagc gaaatacgtt
     7081 acgcatagtg tcaggcttta agcctgcctc tctggataat tgagatagtg tcttcccctt
     7141 catacgaaca gcagccttta tctgctctgc gtgccagtct tggttttttg ctgacattct
     7201 gcgatcctta ctctcttaac gttttcggta actgataccg gctaccgcta tcgggaaaaa
     7261 cgatatcgca gaaaaatgcg catttcaaga gctatgcaca tttttatttt aaccaatgca
     7321 aatcaaagaa ttctgtaaaa ttctttatat aacagagaga ttgagcatga caggtgaaaa
     7381 agaaagcaaa aatgcagagc gcatttttat gccccggaaa taatgcgttt caaggaacgc
     7441 cttgaagagg caatgaatgg agaa
//
LOCUS       DAJTYF010000161         7460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTYF010000161 DAJTYF010000000
VERSION     DAJTYF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.161"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..233)
                     /locus_tag="OO590_003733"
     CDS             complement(<1..233)
                     /locus_tag="OO590_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR6638923.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAPIFP"
     gene            753..1448
                     /gene="minC"
                     /locus_tag="OO590_003734"
     CDS             753..1448
                     /gene="minC"
                     /locus_tag="OO590_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6638924.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1472..2284
                     /gene="minD"
                     /locus_tag="OO590_003735"
     CDS             1472..2284
                     /gene="minD"
                     /locus_tag="OO590_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6638925.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2288..2554
                     /gene="minE"
                     /locus_tag="OO590_003736"
     CDS             2288..2554
                     /gene="minE"
                     /locus_tag="OO590_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6638926.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2926..3270)
                     /locus_tag="OO590_003737"
                     /pseudo
     CDS             complement(2926..3270)
                     /locus_tag="OO590_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3305..3424)
                     /locus_tag="OO590_003738"
     CDS             complement(3305..3424)
                     /locus_tag="OO590_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6638927.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3385..3570)
                     /locus_tag="OO590_003739"
     CDS             complement(3385..3570)
                     /locus_tag="OO590_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638928.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3671..3844
                     /locus_tag="OO590_003740"
     CDS             3671..3844
                     /locus_tag="OO590_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638929.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3846..4190
                     /locus_tag="OO590_003741"
     CDS             3846..4190
                     /locus_tag="OO590_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6638930.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4194..4529
                     /locus_tag="OO590_003742"
     CDS             4194..4529
                     /locus_tag="OO590_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6638931.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4586..6907)
                     /locus_tag="OO590_003743"
     CDS             complement(4586..6907)
                     /locus_tag="OO590_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6638932.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPIPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7024..7233)
                     /locus_tag="OO590_003744"
     CDS             complement(7024..7233)
                     /locus_tag="OO590_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638933.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2047 a         1753 c         1655 g         2005 t
ORIGIN      
        1 tctggaaata taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga
       61 tatttccttg ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa
      121 acaacgccgc acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag
      181 aacatcatcc ccacacctga caatccgata taaataatac gcatcatatt catagcacat
      241 cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc
      301 gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta
      361 tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg
      421 caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg
      481 gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa
      541 taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat
      601 gaatcagcgc catttatcac agaatagact tttactctga ataaatggga gggtgacttg
      661 cctcaatata atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg
      721 cgatgattaa tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag
      781 gcagtagctt cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc
      841 aggcgctgaa agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac
      901 tcaacgtcag tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg
      961 caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa
     1021 ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc
     1081 ccgcaccgca ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata
     1141 ccccggtgcg ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc
     1201 acgttagcgc tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc
     1261 gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc
     1321 tgatggcgga attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag
     1381 aattttatgg caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt
     1441 taaattgatc cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg
     1501 taaagggggt gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa
     1561 gggaaagaaa actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat
     1621 gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct
     1681 aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca
     1741 aacacgcgat aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa
     1801 agcgatggat tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt
     1861 aatggcactc tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt
     1921 acgcgactct gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg
     1981 cgaagagcct attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag
     2041 aggtgacatg ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt
     2101 gatcccagag gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga
     2161 cattaacgcc gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga
     2221 acgtcctttc cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg
     2281 ataagttatg gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc
     2341 aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta
     2401 tctgccgcag ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc
     2461 tgagatggta accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa
     2521 cgtgacctta ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt
     2581 caaggcagag tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc
     2641 ggcgagtatt cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag
     2701 gctgttccca cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta
     2761 gtgtggatat gctgggagat gataaccccg caatttcatt attcattgtt ctcccatagg
     2821 atgaggtttc ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat
     2881 cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt
     2941 cacaccagcc accgcgttcc acggggactc cacaccggca ccatggctat accccacccc
     3001 gaaatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg
     3061 tctgcccgac aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc
     3121 gacaaattct ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag
     3181 acgcatgttg taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga
     3241 tttcgattcc atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc
     3301 aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat
     3361 tgtgttcacc gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag
     3421 gcatttttta cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata
     3481 atactgtcgt acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt
     3541 aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc
     3601 agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata
     3661 ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat
     3721 tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt
     3781 taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat
     3841 ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc
     3901 cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt
     3961 tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg
     4021 gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg
     4081 ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag
     4141 gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga
     4201 tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg
     4261 actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg
     4321 aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca
     4381 gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag
     4441 aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg
     4501 acaccatgcg tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga
     4561 cgcggttttt tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg
     4621 tgtcattgta gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg
     4681 gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt
     4741 cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt
     4801 caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa
     4861 aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc
     4921 cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct
     4981 ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt
     5041 aacccgcttc cagcgagacg gttgcacctt tcgcgctata ggattcatag gctaattcat
     5101 cccctttcac ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt
     5161 ttttactgat gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac
     5221 tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac
     5281 cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc
     5341 cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc
     5401 aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta
     5461 caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt
     5521 tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg gggataggcg
     5581 caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt
     5641 tagtcagata ccagttttta ttggtcgctt cggtattacc tcgggttaat gaatattcgt
     5701 aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc
     5761 tgataatctc tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa
     5821 ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat
     5881 catccaattc agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg
     5941 ttaatcttgt tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat
     6001 caaccgtgag gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa
     6061 gcgacagata ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg
     6121 aattagttgc atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg
     6181 atgatacctc actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt
     6241 catttgctga ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta
     6301 tggaagactc cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg
     6361 tcaccgtgcc gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt
     6421 ctccctggtt aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat
     6481 gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac
     6541 cagccttgac gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga
     6601 tctccccttt tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg
     6661 cagtactttc ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg
     6721 caagaacgcc gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa
     6781 aagagggggt aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc
     6841 cactcgtttg acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac
     6901 cctgcaaaat aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgggc
     6961 ttagaggact attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa
     7021 tggttaactt actaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat
     7081 aactagcgca ttatcaatat cttttacttt tccatctttt attgccacag catgtttata
     7141 ttcatcgtgc tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc
     7201 caaactaaac ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa
     7261 caaacattcc tggtcaaata acctatgagc tcagattacc aaaacggagg gtaaagacaa
     7321 aaacaaaagc acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag
     7381 tcggcattat catttttcac cttattttca tgacattgat cactttgggg tgactatttt
     7441 gtttaacaaa taactcttcg
//
LOCUS       DAJTYF010000162         7391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTYF010000162 DAJTYF010000000
VERSION     DAJTYF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.162"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..1104
                     /locus_tag="OO590_003745"
     CDS             <1..1104
                     /locus_tag="OO590_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR6638934.1"
                     /translation="GEVETLNGKLTELENLKSDLEAELAEVKRPAGGTQNKVAGEHKE
                     AFIGFMRKGREDGLRELERKALQVGNDEDGGYAIPEELDRTILTLLKDEVVMRQEATV
                     ITLSGSDYKKLVNLGGTTSGWVGETEARPETATSKLGLIEPFMGEIYGNPQATQKMLD
                     DAFFNVEDWINSELALEFAEQEEIAFTSGDGSKKPKGFLAYESTDEDDKTRAFGKLQH
                     IASGAASGVTADAIIKLIYTLRKAHRSGAKFMMNNSSLFAIRLLKDNDGNYLWRPGIE
                     LGQPSSLAGYGIVENEQMPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFV
                     GFYTTKRTGGMLVDSQAIKLMKIGAATRQKAAA"
     gene            1149..1472
                     /locus_tag="OO590_003746"
     CDS             1149..1472
                     /locus_tag="OO590_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR6638935.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            1469..1885
                     /locus_tag="OO590_003747"
     CDS             1469..1885
                     /locus_tag="OO590_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6638936.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            1851..2375
                     /locus_tag="OO590_003748"
     CDS             1851..2375
                     /locus_tag="OO590_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638937.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            2372..2935
                     /locus_tag="OO590_003749"
     CDS             2372..2935
                     /locus_tag="OO590_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638938.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLSNERDEKGQWAAYDAVHDVRRELWKALLG
                     WMPDPQGGEIVYVGGTLLDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLS
                     IDVDYIDPGTGPDGDIEHHLEMRFPQN"
     gene            2945..3106
                     /locus_tag="OO590_003750"
     CDS             2945..3106
                     /locus_tag="OO590_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6638939.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            3103..4599
                     /locus_tag="OO590_003751"
     CDS             3103..4599
                     /locus_tag="OO590_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6638940.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAH
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            4599..4955
                     /locus_tag="OO590_003752"
     CDS             4599..4955
                     /locus_tag="OO590_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6638941.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            4955..5291
                     /locus_tag="OO590_003753"
                     /pseudo
     CDS             4955..5291
                     /locus_tag="OO590_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            5376..7325
                     /locus_tag="OO590_003754"
     CDS             5376..7325
                     /locus_tag="OO590_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6638942.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
BASE COUNT         1843 a         1671 c         2192 g         1685 t
ORIGIN      
        1 ggagaagtgg aaaccctcaa cggaaaactg actgaactgg aaaatctcaa aagcgatctt
       61 gaggctgagc tggctgaagt taagcgtccg gcaggtggta cgcaaaataa agttgccggg
      121 gaacataaag aagcgtttat cggttttatg cgcaaggggc gtgaagatgg cctgcgtgag
      181 cttgagcgaa aggcactgca ggtgggtaat gatgaagatg gtggttatgc cattccggaa
      241 gaactggatc gcaccattct gacgttgctg aaagatgaag tggtgatgcg ccaggaggcc
      301 accgtaatca ctctgagtgg ctcggattat aaaaaactgg tgaatctggg cggcacgacg
      361 tccggatggg tgggggaaac ggaagcgcgt ccggaaaccg ccacctcaaa actgggactg
      421 attgagccct ttatggggga aatctacggc aacccgcagg ccacacagaa aatgctcgat
      481 gatgctttct ttaatgtgga agactggatc aacagtgagc tggcgctgga atttgccgaa
      541 caggaagaaa ttgcctttac cagtggcgac ggtagcaaaa aaccaaaagg ttttctggct
      601 tatgagtcca ccgatgaaga tgacaagacc cgtgcgtttg gcaaacttca gcacattgct
      661 tccggcgcgg cttccggcgt gaccgctgat gcgatcatta aactgattta caccttgcgt
      721 aaggcgcacc gcagcggcgc gaagttcatg atgaacaaca gcagcctgtt tgccattcgt
      781 ctgctgaagg ataacgacgg aaattatttg tggcgtccgg gtattgagct gggtcagcct
      841 tcttctctgg cagggtatgg catcgttgag aatgagcaga tgccggatat tgccgccgat
      901 gcaaaagcca ttgcgtttgg gaacttcaaa cgcggctata ccatcgttga tcgcatcggt
      961 acccgtatcc tgcgtgaccc gtacaccaac aaaccgttcg tgggttttta taccaccaag
     1021 cgaaccggcg gtatgctggt ggattctcag gcgattaagc tgatgaaaat tggggctgca
     1081 acccgccaga aagccgctgc gtaatgcggt tttttgtgcc cgcgtaatcg cgggcacagg
     1141 aggaaaatat gctcctgaaa gaagaggaga ttaaatctca tctccggctc gatgatggtt
     1201 tgtacagtga cggcgatttt ctgaaactgc tggcacaggc ggtacagaaa aaaacagaga
     1261 catacctgaa caggaagttg tatgcaccgg aagagacgat tccggaagat gatcctgacg
     1321 ggatgcatct gactgatgac gttcgtctgg caatgctgat gctggtcagt catttttatg
     1381 aaaatcgctc aacgatcacc gatgtggaga aactggaaac gccaatgagt ttcagatggc
     1441 ttgctggccc ttacaggatt gtcccgctat gaaaatcagg caaagtcaga ccagtgccac
     1501 ttacctttta ccggatcccg gagagctgaa tcgccggata aagatccgcc ttcgtgtgga
     1561 tgaacccacc gctgattttg gtacggagcc aacgtatccg gagtcgtttg atgtctgggc
     1621 aaaggtggct cagccaggcg ctgccgctta tcagggctca gtgcagacag aaaatatcgt
     1681 tacgcattat tttacgatcc gtttccggca cgacatcacg gcagatcacg aagtggttta
     1741 ttacggccaa gagtaccgga tccggcgaat acgtgacctg aacggtcagc ggcgtttttt
     1801 attactggaa tgtgaagaac tgcgtacagc gcgacgacgg ggtgaatgcc atgaatcaga
     1861 cagcattttt acacgtcgac tttaaacaac cagaggagct ggagtttaat cgcgcccgtc
     1921 tgcgtcgggc gtttgtgcaa atcgggcgcg tttacatgcg tgatgccaga cgtctggtga
     1981 ttaaacgcgg acgctccggt ccgggagaga acccgggata tcagaccggg cgtctggccc
     2041 gctccattgg ttattacgtt cccaaaaaaa cgacgcgtcg ccccggactg atggtgaaaa
     2101 tttcccctga ccagaaaaac gggcagggga atcgccgttt tcctgaaggt gctccttatt
     2161 atccggcgtt tctgtattac ggcgttcgcc attcagcgta tggaatggat aaaaaggata
     2221 agcggcagaa gaagcaccat tcttcgactt tccggctggc cccgcgtaat aactttatgg
     2281 ctgacgttat tgagcgtcag cgtcactgga cgcaggaact gttgtccagt gagctgcagc
     2341 gttcgttacg tccggtaaaa aggaagcata aatgaaactg acaacgataa ttgcggcgct
     2401 gcgtgagcga tgcccgcgtt ttgaggagcg tgttggtggc gcggcgcagt ttaaggcgat
     2461 ccctgatgcc ggaaaacttc gcctgcctgc tgcctatgtg gttccctctg acgatgcgcc
     2521 gggggaacaa aaatcacaga ccgattactg gcaggatttg actgaaggct tctccgtgat
     2581 tgtggtgctc agcaatgagc gtgatgaaaa agggcagtgg gcagcctatg acgccgttca
     2641 tgatgtccgg agagaactct ggaaagccct gcttggatgg atgccggacc cgcaaggggg
     2701 cgagattgtt tatgtcggtg gcaccctgct ggatctgaac cgttacgaac tgtattacca
     2761 gtttgatttt acggcgaagt atgaaatcac ggaagaagac acgcgacagg cagaggacgt
     2821 gaatgccctg ccggatttat ccctgctgag tattgatgtg gattacatcg atcctggtac
     2881 tggcccggat ggtgacattg agcaccatct ggaaatgcgt ttccctcaga attaagagtc
     2941 cctcatgttt gtgaaacccc tgaaagggcg gtcggttcct gaccctgccc gtggagacct
     3001 tttgccttcc gaagggcgaa atgtggaaga aagcagctac tggttccgcc gtatagcggc
     3061 gggtgatgtg gtacgtgtta aacaggataa ggctaaaaaa tcatgacgat aagttttaat
     3121 actgttccgt cgaatacgct ggtgccgttg ttctacgccg agatgaacaa ttctgcggca
     3181 aatacagcgg tgaccagcgc gcctgcatta ctgatcgggc atgccagcaa cgatgcagcc
     3241 attgaggtta acagcctggt gctgatgcca tcggcagatt atgcccgcca gatttgtggg
     3301 gcggggagcc agctggcgcg tatggtggaa gtctaccgtc agacagatcc tttcggtgaa
     3361 ctgtatgtta ttgcagtacc ggaagccaga ggggcggcgg cgacggtcag ggtgacggtt
     3421 gccggagaag cagaggaaag cggcaccctg agtctgtatg tcgggcgctc ccgtgtacag
     3481 gtgcctgtgg tgaatggcga tgatgccact gcggttgcca ccgcgattaa ggaagcggta
     3541 aacggggtta tcaccctgcc gtttacggcg tcatctgatg caggcgtggt gacgctgact
     3601 gcccgtcata aggggctgta tggtaatgag ttaccggtct gtctgaatta ttatggttct
     3661 ggtggtggtg agattctgcc agcagggctt cgggtggtga cggaagtcgg tgctgccggt
     3721 agcggagcgc ctgatcttac cgccgctgtt gctgctatgg gcgatgaggc attcgacttt
     3781 atcggtctgc cgttcaacga tgccgcctcc atcaatatga tgatgaccga aatgaatgac
     3841 agcagcggtc gctggagtta tgcgcgccag ttatacgggc atgtctatac cgcaaaactg
     3901 ggaacgctgt cagagctggt tggtgccgga gatatgcata atcagcaaca tatcacgctt
     3961 gccggttacg aaaaagaaac ccagtcgcct gtcgatgaac tggttgccag tcgccttgcc
     4021 cgtgaagcgg tatttatccg gaatgatcct gcccgtccga cacagacggg ggagctggtg
     4081 gggatgcttc cggcaccgaa aggtaagcga ttcatcatga cagagcagca gaccctttta
     4141 tctcacggcg tggcgacggc ttatgtggaa ggcggcacgt tgcagatcca gcgttccgta
     4201 accacctaca aaaagaatgc gtatggcgtg gcagacaaca gctatctgga cagtgaaact
     4261 ctgcatacca gtgcacatgt tcttcgcaaa ctgaaatcgg tcatcacaag caagtacgga
     4321 cgtcacaagc tggcaaatga tggtacccgt tttggtccgg ggcaggcgat tgtcactcct
     4381 gccgttatca aaggggaact tctggcgaca tatcgtcaga tggagcgtgc cggtattgtg
     4441 gaaaattacg atctgtttaa acagtatctg atagttgagc gtgatgcaga taacccgaac
     4501 cgactgaata cgctgttccc gccggattat gttaatcagt tacgtgtctt tgcggtggtt
     4561 aaccagttcc gtcttcagta ttcagaggag tcagcataat ggcaaagatc gccggaacct
     4621 gtttttttaa agtggatggt cagcagttat cgctgacagg tggcattgaa gtgccgatga
     4681 acaccaatgt ccgggatgac atcgtcggca tggcagggga tgtggattac aaggagacct
     4741 ggcggtcacc ttacgttaag ggcacgttta aagtgcccaa aaactttccg gtcgacaaaa
     4801 ttaccacctc agaccagatg acgattaccg ctgagctggc aaacggcatg gtgtatgtgc
     4861 tttcggcggc atggctgcac ggagaggcta atcataatgc cgaagaaggc acggcagatc
     4921 ttgaattcca cggcgaagag ggagggtatc agtaatggca atgaacgtta cagaaatagt
     4981 tttaaaaaaa ccggtgacag cgcataacga aatgctgcat gtgctggagt tgcgcgagcc
     5041 cacgtatgac gaaatcgagg cgctgggttt tcctttcatt atttccggtg aaggcagtat
     5101 taaactggac agccaggtgg cactgaaata tatcccgttg ctggcgggga tcccgcgttc
     5161 atcggcggcg cagatggcaa aactggatat ttttaagacc agcatgcaga tcctgcgttt
     5221 ttttacccag tcggagacgg gaagcacctc cgggaaacga ctctacaatg ttgcctggtt
     5281 ctggaaactg aatccactgg agctgcgacg ggtggctgtt tcgcagttta cagaactgga
     5341 agccgaggcc gtccgcatta acgaggagat gaagcatggc tgacagtttt cagctgaagg
     5401 cgatcatcac tgccgtggat aaggtatcgg caccgctgaa gggaatgcag cgccagctga
     5461 aaggttttaa aaaggagttt gccagcctgt cactgggcgc agcgggtgcc ggaaccgcag
     5521 tactgggggc gctggcgctc ccggtcaaat ctgccattgc ccttgaatca aaaatggcgg
     5581 atgtccggaa agtggtggat ggtctggata cgccggaagc gtttaaggca atgacggagc
     5641 aggtgcgcga cctgtcaaca gaactgccca tgtcggcgga aggtatcgcc gaaattgtgg
     5701 cggcgggtgg tcaggctggt atcgcccgtg acgagctgat gcagtttact gacgatgccg
     5761 tgaagatggg cgtggccttt gacgccacgg cagaagaatc cggtcagatg atggcgcagt
     5821 ggcgcactgc ctttaaactg acacagggag aggtggcagg gcttgcggat aagattaact
     5881 atcttggcaa taccggtcct gcaagtgcga aaaagatttc tgatgttgtg acccgtattg
     5941 gccctttagg cagtgttgcg ggtgtggcct ccggagagat tgccgcaatg ggggcaacca
     6001 ttgccggaat gggggtggag tcagaaattg ctgcgacggg gataaagaat tttatgttgt
     6061 cgctgacagc gagggattct gccaccaaat cgcagaaaaa agtgctccgc tcgttgcgca
     6121 ttagcccgaa aaaactggcg gcggatatgc agaaagacgc ccgcggggcc atgctgcacg
     6181 tactggattc tctggcaaaa gtgccgaaag aaaaacaggc tgctgtgctg aaagcgctgt
     6241 tcgggaagga atccctgggg gccattgcgc cgctgctcac gaatctggat ttgttgcgaa
     6301 ccaactttaa tcgtgtggcg gatgcgcagc agtatggcgg ctcaatgcag aaagaatatg
     6361 ccgcccgtgc cgcgacgaca gaaaatcagt tgttgctgct gcagaaccag atcaatgcga
     6421 tttcttccac gctgggggaa accttcctgc cttcactcaa tgaaggcata aaagagatga
     6481 agccttttct ggaagaagtg cgcacgtttg tcagagaaaa tcctgaggtc gtaaaaacca
     6541 tcgcgaaaac tggtgcggca ttactgacga tgggcgtggc gattggcaca ttgacacgca
     6601 taacaaaaat catgggtagc gtgatgaata tgacgccggc aaagggatta attgcgcttc
     6661 tcgtcggtgg tgcatatctc ataattgata actgggaaac ggtcggtccg gttgtgaaaa
     6721 aagtctggca ggaggttgat caggttgtca gagcaatggg gggatgggaa caggcagtaa
     6781 aaacaatagc aacggtttct gccctgtata ttggtgtgaa ggcggtggca agtattcgtg
     6841 cggcaacagt cgcccagaat caatggacaa cggcggcagg aaaaacaggg ctgaaattaa
     6901 agggcctcgg aaagatcagt ctgattggtg gattgcttga actgggcatg atggcgcagg
     6961 agtttgaaaa agaacatccc tggcttgtta aaaattttgt ggctgatgca ctgaacagtg
     7021 gatttggtct gaatgataag ttcgacgagt ggggcaaaca gttccatgat tttgtctatg
     7081 acatgaccgg atggcagatg ccacgtggtg atgggtattt atctcccgat aaacgctaca
     7141 ccccgaatgt atcactggaa aggaatcagc tgttaagcct tgcatcttca ccggcaaccc
     7201 gcagcgaact taaggtgacg tttgacaacg cgccgcctgg aatgcgcgtt atcgatttgc
     7261 cgaaaacagg cgatcccttt atgaaaatca cccatgatgt tgggtattct ccatttaaaa
     7321 ggtgagttct ttttttgtgg tgaaagatga ggcaaaatta tcgcgtaaca tgctaacaat
     7381 ggagtttgtt t
//
LOCUS       DAJTYF010000163         7378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTYF010000163 DAJTYF010000000
VERSION     DAJTYF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.163"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            27..878
                     /gene="hchA"
                     /locus_tag="OO590_003755"
     CDS             27..878
                     /gene="hchA"
                     /locus_tag="OO590_003755"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6638943.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(986..2344)
                     /gene="hprS"
                     /locus_tag="OO590_003756"
     CDS             complement(986..2344)
                     /gene="hprS"
                     /locus_tag="OO590_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6638944.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="OO590_003757"
     CDS             complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="OO590_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6638945.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3148..3561
                     /gene="hiuH"
                     /locus_tag="OO590_003758"
     CDS             3148..3561
                     /gene="hiuH"
                     /locus_tag="OO590_003758"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6638946.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3670..4674
                     /gene="msrP"
                     /locus_tag="OO590_003759"
                     /pseudo
     CDS             3670..4674
                     /gene="msrP"
                     /locus_tag="OO590_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4675..5309
                     /gene="msrQ"
                     /locus_tag="OO590_003760"
                     /pseudo
     CDS             4675..5309
                     /gene="msrQ"
                     /locus_tag="OO590_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5567..6217
                     /gene="zinT"
                     /locus_tag="OO590_003761"
     CDS             5567..6217
                     /gene="zinT"
                     /locus_tag="OO590_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6638947.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMISIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7295..>7378)
                     /locus_tag="OO590_003762"
     CDS             complement(7295..>7378)
                     /locus_tag="OO590_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6638948.1"
                     /translation="KKYRVLLNRVDTSTAPDIEWPEEPDTM"
BASE COUNT         2078 a         1679 c         1544 g         2077 t
ORIGIN      
        1 aactaagcaa caataaggaa tacactatga ctgttcaaac aagtaaaaac ccgcaggtcg
       61 atattgctga agataatgcg ttcttccctt cagaatattc gcttagccaa tatacgagtc
      121 ccgtctctga tcttgatggc gtggactatc caaaaccgta tcgcggtaaa cataaaattc
      181 tggtgatcgc cgcggacgaa cgttatttgc cgaccgataa cggaaaactg ttctcgaccg
      241 gtaaccatcc gattgaaacg ttgctgccgt tgtatcatct ccatgctgca ggtttcgaat
      301 tcgaagtggc gaccatttcc ggtctgatga ccaagtttga atactgggct atgccgcaca
      361 aagatgaaaa agtaatgcca ttctttgaac agcataaatc gttattccgt aatccgaaga
      421 aactcgcgga tgttgtcgcc agcctcaacg ctgatagcga atatgcagca atctttgttc
      481 ctggtgggca tggcgcactt attggtttac cagaaagcca ggacgtggct gccgctttac
      541 agtgggcaat caaaaatgac cgttttgtta tctccctttg ccacggtccg gcggcttttc
      601 tggcacttcg ccacagcgat aacccactga atggttattc tatttgcgca ttcccagacg
      661 ccgcagacaa acaaacgcca gatattggct atatgccggg tcatctcacc tggtacttcg
      721 gcgaagagtt gaagaaaatg ggcatgaaca tcattaatga cgacatcacc gggcgagtac
      781 ataaggaccg taaacgtctc accggcgaca gtccttttgc agcgaatgcg ttgggtaaac
      841 tggcggcgca ggaaatgctg gcagcttacg cgagttaatc cctgttacat tgtgatgtaa
      901 gcgcataatc aatcgcagcc acgttaatac tcacgacgtg gctgcgtttt ttcacggtta
      961 acttatggcg tactgaagcc cgatgttaat ttctttgcgg taacgtaatt cggaacacgt
     1021 tatgcttact gagatagtga tacgtagcac ttcctccatg taattcggca atcgctttga
     1081 ctaaagaaag gccaagtccc tgacctacgg aatgacgcga attatctccc cgccaaaatc
     1141 tacggaagag tttttcaggc tcattaattt tcgttccagg actggcgata tcaatattaa
     1201 gagagccgtt ggcatcaaga aaactggtta tatgaatacg cgatttttct ggtgaatatc
     1261 gaatggcgtt aacaataaga ttcgataaca ttcgttgcag taaaatttta tccgcaaaga
     1321 tttgttgatt gcacttgacc ttaaagcaaa tctctttctc gtctgaaaga gattcaagat
     1381 agtccaaaag attttcaact tccttattga gagaaagcga atccagtttc accaaaacat
     1441 tgtttttgtc tgccctggca agaaacagta tattctctgt taaccgcgaa atattttcca
     1501 gctcttcaat gtttccggca attgtttttt gatattcagc gatacttctg atttgactga
     1561 gcgtaacctg attctgaccc agtaatgcat taattggcgt tctaagttca tgagcgagat
     1621 cgtcagcaaa ttgactaagg cgctcaaaat ctttgactaa agcatgatgc attttattca
     1681 acgcctgccc aagaggtttt agttctctcg gtaatgcgtt aacctcaacc ggcactcggc
     1741 tatcgttata attcagcgct tccgttacgc cactcaactt tttgatctct cgcaaccccg
     1801 tcctgattaa caacggactt aatactgagc acagtataat ggcgacaatg caaattataa
     1861 tgctattaat tttatactgt tcaagcatat tatgtctggc tgaagccaat ttagccacag
     1921 taaccgttaa tgatggcgaa acttcatcaa tgttaattcg taaagcatct atctctgtat
     1981 cattaataat gcttctgtaa atgcctgctg cgctgattgt ctcactagca ggtatgttat
     2041 ttaacatgtc atcactgaca tttgtccggt taacaatttt attgatgcca tcactatgaa
     2101 tgatcaaaat atcctgacta acatccatca tccggttaaa gtacacaggt aacgtatctg
     2161 gatttacccc atcaattaac aactgcttga tctgcgctgt ccggttaatg agtgttgtat
     2221 catcgcgcca tttcaactca cttgccaggc cattatagag agtccataca attccagcgc
     2281 cagcaacaga ctgcagaaat ataaaaagca aggttaaacg gacggttata gataatcttt
     2341 tcattatttt tttaccgcta cgaatgaata gcccatcccc cggattgtgg cgattagctt
     2401 ttcaggaaaa ggatcatcaa cttttgcgcg gagcctgcga atggcgacat ccaccgtatt
     2461 ggtatcacta tcaaagttga ttccccaaat ttcactcgca ataaccgttc tgggtataat
     2521 ctcgccagct ctggaggcca gtagccaaag taactgaaac tccttgcgcg tcagtgtaat
     2581 actgatattg tccctgctga cactttgact gacagagtcc atttttaagc cactgatttc
     2641 taatgttgaa ttcagagcgt gatgttgcct taattgtgcc cgaacccttg ctaacaactc
     2701 agaaaatgaa aaaggtttta ccagataatc atttgcccca ctgtccaacc ctctgactct
     2761 gtcatcgaca gaatcccttg cagtaaggca aataacaggg gtttgctttg ccgttcttaa
     2821 cgtttgtaag atctgccagc catccatacc cggaagcata atatccagaa tgatcaatgc
     2881 ataatcatcc ttcagcgcaa gataaagccc atctctgcca tcagaaacgg catcaatgac
     2941 ataacccgct tcggaaagcc cctgcgttac ccattcctgg gtcctttgat tatcttcaat
     3001 aagtagaatc ttcatgaaaa taatatgcca taaatcatat gttatagaaa caacctggtt
     3061 cattacaaaa ttgtaatgct gctgtaaggt taccctggac gttttttcgc tatcctcaaa
     3121 actcattcac atgacaagga tataaacatg ttaaagcgtt atttagtact ctcggtagca
     3181 acggcagcat tttcattacc ttctttggtt tatgccgcac aacaaaacat tcttagcgtg
     3241 cacattttga accagcaaac aggaaaaccc gctgccgacg tgacagtcac tcttgaaaag
     3301 aaagcggata acggctggtt acaacttaat accgccaaaa cagataagga tggacgaatt
     3361 aaggcactgt ggcccgagca aactgcgact actggcgatt accgtgtcgt atttaaaacc
     3421 ggggactatt tcaagaaaca aaatcttgaa agtttcttcc ctgagattcc cgttgaattt
     3481 catattaata aagtgaacga gcattatcat gtgcctttgt tactcagcca atatgggtat
     3541 tcaacctatc gtggcagtta atgcgtaaat atgtaaaagc tgatctctgc tactgttagg
     3601 gaggtgactc cctgacagat taacagtaaa cagctcttgt tggttaacga caaaaaagtg
     3661 tgatggctta tgaaaaagaa tcaattttta aaagaatcag atgttacggc cgagtcggta
     3721 ttctttatga agcgtcggca ggtgttaaaa gcactgggca tcagcgcagc tgcattttct
     3781 ttgcctcacg ctgcgcatgc cgatctgctt agctggttta aagggaacga tcgtccgccc
     3841 gcccccgccg gaaaaccgct ggagttcagc aagcctgccg cctagcaaaa taacctgcca
     3901 ctgacgccag cagataaagt ctccggttat aacaacttct atgaattcgg gctggataaa
     3961 gacgatcccg cggctaatgc tggtagcctg aaaaccgatc catggacact gaaaatcagc
     4021 ggcgaagtgg caaaaccatt gaccctcgat catgatgatt taacccgtcg cttcccgctg
     4081 gaagagcgta tttatcgtat gcgctgcgtg gaagcgtggt cgatggtggt gccgtggatt
     4141 ggttttccgc tacacaaatt gctggcgctt gccgaaccca ccagcaatgc gaagtatgtc
     4201 gctttcgaaa cgatttatgc gccggaacag atgcctggcc agcaggaccg ctttatcggc
     4261 ggcgggctga aatatcctta tgtcgaagga ttgcgtctcg acgaagcaat gcatccgctc
     4321 acactgatga ccgtaggtgt ttatggcaag gcgttaccac cgcaaaatgg cgcgccggtg
     4381 cgactgattg tgccgtggaa atatggcttt aaagggatta aatcgatcgt cagtattaag
     4441 ctgacccgcg agcgtccgcc aaccacctgg aatctggcag cgcctgacga atacggtttt
     4501 tacgccaacg ttaatccgca tgttgatcac ccgcgctggt cacaggctac cgaacgattt
     4561 attggttcag gcggcattct cgatgttcag cgccagccaa cactactgtt taatggttac
     4621 gccgaccagg tggcatcgct gtatcgtggc ctggatttgc gggagaattt ctaaatgcgt
     4681 ctgacagcaa aacaggtgat atggctgaaa gtttgcctgc atcttgccgg attgttgccg
     4741 tttctctggc tggtctgggc gatcaatcac ggtggactgg gtgccgatcc ggtgaaagat
     4801 attcagcatt ttaccggtcg cactgcactg aaatttttgc ttgcggcctt gttaatcaca
     4861 cctctggcac gctacgcaaa acagccgtta ttgatacgca ctcgccgcct gttaggctta
     4921 tggtgctttg cctgggcgac actgcattta accagttacg cattgctgga gttgggcgtg
     4981 aataatctgg cgttattagg taaggagtta attacccggc cttatttaac gttaggaatt
     5041 atcagctggg ttattttgct tgctttagcg ttcacttcaa cccagtcgat gcagcgaaaa
     5101 ctcggcaaac actggcaaca gttgcataac ttcgtctatc ttgtcgcgat cctggcccca
     5161 atacattatc tgtggtcagt gaagattatt tcaccgcagc cactcatcta cgctgggctg
     5221 gctgtactgc tttagcctta cggtataaga agttgctttc ccttttcaac cggttacgca
     5281 agcaggccca caacaaatta tctctataac ttcatattgc cgacaaactt cgcgttatcc
     5341 cggccggatg cggcgcgagc gccctatccg gcccacacag atcgcatcaa gttatgtttc
     5401 gtttgtcatc aacctttcat caccagaata atttatttta tttcaataag atagttcaat
     5461 acacctgaga ataccatgct ctcatttcct aatgagttgt tgcatttttt catatgttac
     5521 aatataacat tacacataat gtacattaac tctggaggaa attgttttgg cgattcgtct
     5581 tcataaactg gctgttgctt taggtgtctt tattgttagc gctcctgcct tttcgcatgg
     5641 tcatcattct catggcaaac ccttaacaga ggtcgaacaa aaagctgcta atggtgtttt
     5701 tgatgatacc aatgtacaaa accgaacgct cagtgactgg gatggagtct ggcaatccgt
     5761 ttacccttta ctgcaaagtg gcaaacttga tcccgtcttt cagaagaaag cggatgcaga
     5821 taaaaccaaa acatttgctg aaattaaaga ttattatcgc aaaggttatc cgacagatat
     5881 cgaaatgatt agcattgagg acggcattgt cgaattccat agaaataatg aaacaacatc
     5941 ctgtaaatat gattacgatg gatacaaaat actcacctat aaatcaggca agaaaggcgt
     6001 tcgttactta ttcgaatgta aagatcctga aagcaaagcc cctaaatata tacaatttag
     6061 cgatcatata attgcaccac gaaaatcatc tcattttcac atttttatgg gtaatgattc
     6121 ccagcaatct ttattgaatg aaatggaaaa ctggccaacg tattatccct atcagttgag
     6181 tagcgaagaa gtggtcgagg agatgatgtc tcattgattt ttttgcgctg acttcagtta
     6241 tttaacagaa cagtgcctgg tagtaacaac aataatttac caagacaaat aagtaagaca
     6301 aactcagaca cgctcatact tgagtcgtgt cttttttatt atgtctgtag aaaaaaactg
     6361 agaaaaaggc gaacaataaa tacacaactc agccatacag actggcagcg caaatgtttc
     6421 caggagcaag tcatctgtca tggtaaagct aaccagaaat ataggttaat tttgttgttt
     6481 aaaccttttt tacttgttag tataatacaa aggctttcaa aaaagctggg aaattcaaca
     6541 actcttggaa gttatggact ttgtcccgcg gctttaatct gacggccgcg ttcctttttt
     6601 ggctcaatta ttgacccctt ctctacgcaa cttcagttcc cgccagcaac tttgcggcag
     6661 tttcatagga tcaatgtcta aaagaataat ggtgaccgat aagaaaacga ctgaataact
     6721 gtagattttc gctcgaaacc ttcctgtcag ctccatagcg aatcaagcgc tgaatgtcac
     6781 agtatcgaac agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt
     6841 ttaggaaaag caaggcggga agtcgggaga taagtcattg ataaagtggc ggagagaggg
     6901 ggatttgacc ccccggtaga gttgccccta ctccggtttt cgagaccgat gatacagagt
     6961 cgaaaaatca aagagttaaa aatttttctt ggaaaaaatg tcttaaaatc gtacacgaaa
     7021 aatcaatgaa ttacataaca ttaaagaaga atattccaag ccttcttgag tccaccacct
     7081 gttttcaggt gtcattcaga cgtgatttgc cccagattgg gaaagcacgc ttttttctca
     7141 gcttattccg ttttgcctgt gtgttgaata attccactgc agttatataa aatcggtaac
     7201 ggctggaaat cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacgtcc
     7261 tgcatacgtc gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc
     7321 actcaatatc aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttctt
//
LOCUS       DAJTYF010000164         7291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTYF010000164 DAJTYF010000000
VERSION     DAJTYF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.164"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            91..1202
                     /gene="yneK"
                     /locus_tag="OO590_003763"
                     /pseudo
     CDS             91..1202
                     /gene="yneK"
                     /locus_tag="OO590_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            1352..2542
                     /gene="ydeA"
                     /locus_tag="OO590_003764"
     CDS             1352..2542
                     /gene="ydeA"
                     /locus_tag="OO590_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6638949.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2567..3232)
                     /gene="marC"
                     /locus_tag="OO590_003765"
     CDS             complement(2567..3232)
                     /gene="marC"
                     /locus_tag="OO590_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6638950.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3444..3878
                     /gene="marR"
                     /locus_tag="OO590_003766"
     CDS             3444..3878
                     /gene="marR"
                     /locus_tag="OO590_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6638951.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3898..4303
                     /gene="marA"
                     /locus_tag="OO590_003767"
                     /pseudo
     CDS             3898..4303
                     /gene="marA"
                     /locus_tag="OO590_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
     gene            4335..4553
                     /gene="marB"
                     /locus_tag="OO590_003768"
     CDS             4335..4553
                     /gene="marB"
                     /locus_tag="OO590_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6638952.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4584..5483)
                     /gene="eamA"
                     /locus_tag="OO590_003769"
     CDS             complement(4584..5483)
                     /gene="eamA"
                     /locus_tag="OO590_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR6638953.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGTV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5747..6865
                     /gene="ydeE"
                     /locus_tag="OO590_003770"
     CDS             5747..6865
                     /gene="ydeE"
                     /locus_tag="OO590_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR6638954.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            6992..7087
                     /gene="mgtS"
                     /locus_tag="OO590_003771"
     CDS             6992..7087
                     /gene="mgtS"
                     /locus_tag="OO590_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR6638955.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1903 a         1773 c         1659 g         1956 t
ORIGIN      
        1 gcgccacgca tcacctgcct ttgatagttc ttgcagctca aatgccctat atagtgatgt
       61 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac
      121 atatctcttc ctgatgattt tcccgctcgt aatattgcac ctcaagtaaa agaagtttta
      181 aaggatttta ttgatgcact cagtaccata atatgtgatg aagagtggcg cacgtcttta
      241 aacaccaact cagccacaaa aaagatattt aacaaccttg ataacttatc atatattcag
      301 aggacttctt ttcggggtaa cgacacacta tacaatgaaa aggttcagtt taaactcact
      361 tatcccgtca agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta
      421 agccctattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
      481 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
      541 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
      601 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
      661 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
      721 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
      781 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
      841 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
      901 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
      961 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     1021 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactatttg
     1081 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     1141 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     1201 aaaccgtgat gttaccgact ctctgacgcg gaacagaatc agcgtcagag aaacggaaaa
     1261 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     1321 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     1381 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     1441 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     1501 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     1561 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     1621 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     1681 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     1741 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     1801 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     1861 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     1921 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     1981 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     2041 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     2101 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     2161 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     2221 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     2281 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     2341 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     2401 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     2461 ggtgcctgct tttgccgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     2521 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     2581 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     2641 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     2701 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     2761 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     2821 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     2881 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     2941 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     3001 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     3061 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     3121 cactgttcat gttgcctgcc aggccaagaa atagcgcaac agttgttaat gggttagcta
     3181 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     3241 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     3301 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     3361 gcatcggtca attcattcat ttgacttata cttgcctagg caatattatc ccctgcaact
     3421 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     3481 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     3541 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     3601 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     3661 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     3721 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     3781 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     3841 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     3901 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     3961 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatgagcg
     4021 ttcgggttac tccaaatggc acctgcaacg gatgtttaaa aaagaaaccg gtcattcatt
     4081 aggccaatac atccgcagcc gtaagatgac ggaaatcgcg caaaagctga aggaaagtaa
     4141 cgagccgata ctctatctgg cagaacgata tggcttcgag tcgcaacaaa ctctgacccg
     4201 aaccttcaaa aattactttg atgttccgcc gcataaatac cggatgacca atatgcaggg
     4261 cgaatcgcgc tttttacatc cattaaatca ttacaacagc tagttgataa cgtgacaacg
     4321 tcactgaggc aatcatgaaa ccactttcat ccgcaatagc agctgcgctt attctctttt
     4381 ccgcgcaggg cgttgcggaa caaaccacgc agccagttgt tacttcttgt gccaatgtcg
     4441 tggttgttcc cccatcgcag gaacaaccac cgtttgattt aaatcacatg ggtactggca
     4501 gtgataagtc ggatgcgctc ggcgtgccct attataatca acacgctatg tagtttgttc
     4561 tggccccgac atctcggggc ttattaactt cccaccttta ccgctttacg ccaccgcaag
     4621 ccaaatacat tgatatacag cccggtcata atgagcaccg tacctaaaaa ttgcagaccc
     4681 gttaagcgtt catccaacaa tagtgccgca cttgccagtc cgactacggg caccagtaac
     4741 gataacggtg caacccgcca ggtttcatag cgtcccagta acgtccccca gatcccataa
     4801 ccaacaattg tcgccacaaa cgccagatac atcagcgaca agatggtggt catatcaata
     4861 gtaaccagac tgtgaatcat ggatgcggaa ccatcgagaa tcagcgaggc aacaaagaag
     4921 ggaatgattg ggattaaagc gctccagatt accagcgaca tcaccgccgg acgcgttgag
     4981 tgcgacatga tctttttatt gaagatgttg ccacacgccc aactaaatgc tgcagccagg
     5041 gtcaacataa agccgagcat cgccacatgc tgaccgttca gactatcttc gattaacacc
     5101 agtacgccaa aaatcgctaa ggcgatcccc gccaattgtt tgccatgcag tcgctccccg
     5161 aaagtaaacg cgccaagcac gatagtaaaa aacgcctgtg cctgtaacac cagcgaagcc
     5221 agtccagcag gcataccaaa gttaatggcg cagaacagaa aggcaaactg cgcaaaactg
     5281 atggttaatc cataccccaa gagcaaattc agtggtactt tcggtcgtgc aacaaaaaag
     5341 atggccggaa aagcgaccag cataaagcgc aaaccggcca gcatcagcgg tggcatgtta
     5401 tgaagcccca ctttgatgac cacaaaattt agcccccata cgaccactac cagtagcgcc
     5461 aacaccccat cttttcgcga cattctaccg cctctgaatt tcatcttttg taagcaatca
     5521 acttagctga atttactttt ctttaacagt tgattcgtta gtcgccggtt acgacggcat
     5581 taatgcgcaa ataagtcgct atacttcgga tttttgccat gcgtccgatg gttaataaca
     5641 acgacgaggc aagaagggcg ctggtagagc gtcgtaggga taagttcatt tgtctgtccg
     5701 gcagtgcgtt tcgttatgtt ttattttaga gatgtaaaga aatagcatgg ctacgctgcc
     5761 atttatgacc atttacttga gtcgccagta cagcctgagt gtcgatctaa tcggttatgc
     5821 gatgacaatt gcgctcacta ttggcgtcgt ttttagcctc ggttttggta tcctggcgga
     5881 taagttcgac aagaaacgct atatgttact ggcaattacc gccttcgcca gcggttttat
     5941 tgccattcct ttagtgaata acgtgacgct ggttgtgctc ttttttgccc tcattaactg
     6001 cgcctattct gtttttgcta ccgtgctgaa agcctggttt gccgacaatc tttcgtccac
     6061 cagcaaaacg aaaatcttct caatcaacta caccatgcta aacattggct ggaccatcgg
     6121 tccgccgctc ggcacgctgt tggtgatgca gagcatcaat ctgccctttt ggctggcagc
     6181 tatctgttcc gcgtttccca tgcttttcat tcaaatatgg gtaaagcgca gcgagaaaat
     6241 catcgccacg gaaacaggca gtgtctggtc gccgaaagtt ttattacaag ataaagcact
     6301 gttgtggttt acctgctctg gttttctggc ttcttttgta agcggcgcat ttgcttcatg
     6361 catttcacaa tatgtgatgg tgattgctga tggggatttt gccgaaaagg tggtcgcggt
     6421 tgttcttccg gtgaatgctg ccatggtggt tacgttgcaa tattccgtgg gccgccgact
     6481 taacccagct aacatccgcg cgctgatgac agcaggcacc ctctgtttcg tcatcggact
     6541 ggtcggtttt attttttccg gcaacaacct gctattgtgg ggtatgtcag ctgcggtatt
     6601 tactgtcggt gaaatcattt atgcgccggg cgagtatatg ttgattgacc atattgcgcc
     6661 gccaggaatg aaagccagct atttttccgc ccagtcttta ggctggcttg gtgccgcgat
     6721 taacccatta gtgagtggcg tagtgctaac cagcctgccg ccttcctcgc tgtttgtcat
     6781 cttagcgttg gtgatcattg ctgcgtgggt gctgatgtta aaagggattc gtgcaagacc
     6841 gtgggggcag cccgcgcttt gttgatttaa gtcgaacaca ataaaaattt aattcagcct
     6901 tcgtttaggt tatctctgct aatatctttc tcattgagat gaaaattaag gtaagcgagg
     6961 aaacacacca caccataaac ggaggcaaat aatgctgggt aatatgaatg tttttatggc
     7021 cgtactggga ataattttat tttctggttt tctggccgcg tatttcagcc acaaatggga
     7081 tgactaatga acggagataa tccctcacct aaccggcccc ttgttacagt tgtgtacaag
     7141 gggcctgatt tttatgacgg cgaaaaaaaa ccgccagtaa accggcggtg aatgcttgca
     7201 tggatagatt tgtgttttgc ttttacgcta acaggcattt tcctgcactg ataacggatc
     7261 gttgacacag tagcatcagt tttctcaatg a
//
LOCUS       DAJTYF010000165         6901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTYF010000165 DAJTYF010000000
VERSION     DAJTYF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.166"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..91)
                     /locus_tag="OO590_003772"
     CDS             complement(<1..91)
                     /locus_tag="OO590_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HCR6638956.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
     gene            147..299
                     /locus_tag="OO590_003773"
                     /pseudo
     CDS             147..299
                     /locus_tag="OO590_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1680..2627
                     /gene="icsP"
                     /locus_tag="OO590_003774"
     CDS             1680..2627
                     /gene="icsP"
                     /locus_tag="OO590_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6638957.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3189..3561)
                     /locus_tag="OO590_003775"
                     /pseudo
     CDS             complement(3189..3561)
                     /locus_tag="OO590_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3805..3924
                     /locus_tag="OO590_003776"
                     /pseudo
     CDS             3805..3924
                     /locus_tag="OO590_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4174..5040
                     /gene="ospB"
                     /locus_tag="OO590_003777"
     CDS             4174..5040
                     /gene="ospB"
                     /locus_tag="OO590_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6638958.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5369..6109
                     /gene="phoN2"
                     /locus_tag="OO590_003778"
     CDS             5369..6109
                     /gene="phoN2"
                     /locus_tag="OO590_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6638959.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(6462..>6901)
                     /gene="ospC4"
                     /locus_tag="OO590_003779"
     CDS             complement(6462..>6901)
                     /gene="ospC4"
                     /locus_tag="OO590_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6638960.1"
                     /translation="KIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGDKKA
                     DIALYLLSKFNFTKQDIAEMEKMNNNIYCNLYDVEYLLSKDGANYKVLEYFINNGLVD
                     VNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRFER"
BASE COUNT         2164 a         1289 c         1333 g         2115 t
ORIGIN      
        1 caactctagg ataaatattt ctcactatat attcagtatt tgcagtattt attggaaata
       61 cgttcttaat attcttgatg ggactaatca ttttaatctc tatactcaaa tataaacatt
      121 accatgaaca atataattgt tcctaagtgg aatgtctcca cggagaacac tttatctatc
      181 gggaaatagt gcgggcaaca gtgtttaatt atatcgagtg cgattacaat cgctggcgac
      241 ggcaccgttg gtgtggcggc ctcagtccgg aacaatttga aaaccagaac ctcgcttagg
      301 cctgtgtcca tattacgtgg gtaggctcac agagcctcac ttacgactgc gtccatatta
      361 tgtgggtagg atcaataaag acgttttata tgtgtctgct tgcagccaga aaatttatac
      421 cctgcaggta tcataaacat gcttgataac ttaattgggg ctccctcttc cttttatggt
      481 gtaattgttt gattgaatac ttccggggat ttcagtatga aatgaagtat atttaatata
      541 ctttacaaaa agtttgtatt agtggaataa ggacaatagc ttatccaaac tgccaagcct
      601 ctttattata agtaagatct ggcactggaa tattctcaaa aggactaaca tcatcaccac
      661 gaccttcaac caaccatttt attgcattta aatcccagaa tagttcagta gtggtcaatt
      721 tgggaaacgc acaatattgt acggttacgt tatcgcaata gagtgaactc ccgccctata
      781 cggcgttttc aaggattagt ccttaatcgg acaaccacat taaagcataa taaataatta
      841 agctttaata atatactata gatacagttc tattataaaa aatactattt taggtgatgt
      901 attaaattag tagaatattt attcttttat aaaagcacca atgaattgtc caagtaaaca
      961 tgaggtaaaa tgtgtagagg ataactgagt ttggtcactt taactgtatt agtcgcagtc
     1021 tgactctcga ctttaaaagg atgggaatta cctgtactga tagtgagaaa gctttccatt
     1081 gaaataagtc aaataaaagt tgatggcttc agtgctaaag tgaggtgccc gctagccgta
     1141 ttcactcgaa tcggaattca atgtattgct tctgccattt ccataatatt tcacgggaaa
     1201 aacaaaatct gaaaaactat acttaagtgt tgtattaagt cagttgagat acaaatacat
     1261 atttatcagc acacgttacg tcaaatacag gaggttgtca tgttggtact gaaaggcacg
     1321 ttggctattg cagccgtgat gacaatttat tgtcttatcg ttgttcttat ggaacgcttt
     1381 tctgattgat ttcatattgg cgagagaaca agaaggtgag tagaattgtc gcggaatcct
     1441 gaaactatca gcctcaggta tgaacaccaa aaggtaggta gagaaatcta tggcccccgt
     1501 tactttattc gcacaatgag gtattttttt atttttacaa aagttcaata aatattataa
     1561 taaaagatat gttcttggta acataaacaa atatttaatt aaaataatgc ttggcattga
     1621 cgtttatctt ataaagtaag aagatcatgg acatttcaac caaaaaagta gagttctcga
     1681 tgaaatcaaa attccttgta cttgcacttt gtgtacctgc gatctttact acacatgcta
     1741 ccactaacta tccacttttc ataccggaca acatcagtac ggatattagt ctaggatctc
     1801 tgagtggcaa aacaaaagaa cgcgtttatc atcccaagga aggggggaga aaaattagtc
     1861 aactggactg gaaatacagt aatgcgacta ttgttagagg tggcatcgat tggaagctaa
     1921 ttccaaaagt gtctttcgga gtttccggtt ggactacttt aggtaaccag aaagcaagca
     1981 tggttgataa agactggaac aattccaata cccctcaggt atggaccgac cagagctggc
     2041 atcccaatac gcatctccgt gatgctaacg aattcgagct gaatcttaaa ggttggttat
     2101 taaataattt ggattatcga cttggactaa tagcaggtta ccaagagagt cgttacagtt
     2161 ttaatgcaat gggagggagt tatatttata gtgaaaacgg ggggaacagg aataaaaaag
     2221 gggcacatcc tagtggtgaa agaacaatag gttacaaaca gctcttcaaa ataccttata
     2281 ttggattaac tgctaattac cgccatgaga attttgagtt tggagcagaa ctgaaatata
     2341 gtggttgggt tctttcatct gatacagata aacactatca gactgagaca atttttaaag
     2401 atgaaataaa aaaccaaaat tactgctctg ttgctgcgaa tattggatac tatgtcaccc
     2461 ccagtgcaaa attttatata gaaggctcca gaaattacat ttctaataaa aaaggtgata
     2521 catctcttta tgagcaaagt accaatatat ctggcaccat taaaaatagt gcaagtattg
     2581 aatatattgg ttttctcact tccgcaggta taaagtatat tttttgaatt ggttcaccga
     2641 gttgattacg ttttcatggc taaaaattgc cgcccattgt gagtcatatt cgccctgact
     2701 ttccagaacc atacgaactg cccgttgacg gacttcgggg gaaaaacgtg tatttttagt
     2761 cattctgttt acctctttct cagggcgttt agtctccagg atttccgggg cggttcaaaa
     2821 aactcttcgt tgattactgt ggacttactg ttggcattac cgaccctgag accggagaaa
     2881 taagaaccgc ttagatcatc gtggctgttc tcggggtatc aagttacaca tgggcagagg
     2941 ctacccaagg tctactatta tcgcctgaaa gcgctgatgg aacagtgcta tcagggacat
     3001 gctgatgaaa atacctaaat aatcagccag cactctatct ttccaaatcc acagcatagc
     3061 aaagagagca aaagagcctg taaattcaga aattaaggaa acgcctcttg gtgcacataa
     3121 tctatcttat gttaaatgaa atctaatttc ttgattatat gctggatcgt tcgtgggtgg
     3181 ctttgcccct atatttccag acacctgtta tcattggccc gctgccgcag atattcccgt
     3241 ggcgagcgat aatccagcgc actatgcgga tgccattcgt tataatgctc gaatgcctct
     3301 gcaaggttct ttgctgccgt taacccgtca ggtttgggca tgatactgat gtagtcacac
     3361 tttattgttt tcacgaagct ctttgctatt ctgttactct ccggactccg caccgccgtg
     3421 ttcttcggtt caacaaagtc ccaacatccg ggtgaactgg cgtgtttcat tagcccggta
     3481 gcatgaacca ttatccgtca gtcactccac tggagacgac ggaagatcgt tgccgaagcg
     3541 gtgttccacc gctcccagca tgtcgccaat gggagagcat ctacggtctg attgaactgt
     3601 ttattcaaat tgtccaccgt ctgtcagtac gctctgaacg tcggctggtt aaaacactgc
     3661 tcgctgattt tcagagggtt cacggtaaaa cagcactttt attccggatt gcggaagcgg
     3721 ctctgaataa tccggatggg ctggttaaag aggttgtgta ccatctgcat attgttgaac
     3781 cagatcgcag cggtaagaga agttcatcat acttggcgca gttacgcgat gtttctgcta
     3841 gaggtgatgc cgtaaagaac ggcagaacat taccggaaca agatagcggt ctacctgcac
     3901 tggtatcaga cccggggctt ccccggatga tctccacggt actttaggta ctgtgtctat
     3961 attatgtagg taggatcaat taactttgta gttgaaattc tttcattgaa ataatgaata
     4021 aaaaagacaa accgcattaa acaatgccag aatataattg catttttgtt ccgttttttt
     4081 aaatcatagt atacaaagaa gcttgcatac tatatgaaaa tataacaccc tttactatga
     4141 tgtttataaa acaatatatg gagtcatgta ggtatgaatt tagatggtgt tagaccatac
     4201 tgtagaatag tcaataaaaa gaatgaaagc atatcagata ttgcatttgc acatataata
     4261 aaaagggtaa aaaattcatc atgtactcac ccaaaagcag cattggtttt tttaggagag
     4321 aaaggttttt gtgatagcaa tgatgttcta tctattatgg gacaacaaat accaagagta
     4381 tttaagaaca agatgttata tgattatgtt tttaaaaatg aaaaaagtaa aaatgatttt
     4441 ctaaaaatgg ctgaatcatg gctaccacag agtgaaccaa tagtaataaa taatgatgat
     4501 gacgcattga atgctgctgc ttatttttct gtaaaaaaag cgaaaataaa aacagtaaac
     4561 gatactgatt ttaaagagta taataaggtt tatattcttg ggcacggtag tcctggttct
     4621 catcaattag gccttggttc ggaacttatt gatgtacaaa caatcatttc aagaatgaaa
     4681 gactgtggta ttctaaatgt gaaagatatc cgttttactt catgcggctc cgctgataaa
     4741 gtggctccta aaaattttaa caatgcccct gctgaaagtc tttcttgtat ccttaactct
     4801 ctgccttttt ttaaggaaaa agaatctttg ctagagcaga taaaaaaaca ccttgaaaac
     4861 gatgagtcat tgagtgatgg tctaaaaata tccggctatc atggatatgg agttcactat
     4921 ggtcaagagc tttttcccta ctcacattat cgttcaactt caattcctgc tgatccggag
     4981 catacagtaa aaagaagctc tcagaaaaag acttttatta ttaataaaga actggattag
     5041 tataaaattt ttaacctata gttaattgtt tgcatttaag atgaagttat tttatcttta
     5101 gtagaaaagt gcttccctat gagggaaaat cacaaatcat cataatacag agacaaaacg
     5161 atacgaacaa atagataaaa aacatcattc ttttaccaca ttatttttcg tgaatatgaa
     5221 aaataatgtt attactttaa tataagacta ttttttgttt ttccatcact ctgttcaaat
     5281 ttttccgcat gacttgtgtt ttttgtaaca cagctcgttt tttacagctg accaaaatca
     5341 tcaattaatt atgctaagga aataaattat gaaaaccaaa aactttcttc ttttttgtat
     5401 tgctacaaat atgattttta tcccctcagc aaatgctctg aaggcagaag gttttctcac
     5461 tcaacaaact tcaccagaca gtttgtcaat acttccgccg cctccggcag aggattcagt
     5521 agtatttcag gctgacaaag ctcattatga attcggccgc tcgctccggg atgctaatcg
     5581 tgtacgtctc gctagcgaag atgcatacta cgagaatttt ggtcttgcat tttcagatgc
     5641 ttatggcatg gatatttcaa gggaaaatac cccaatctta tatcagttgt taacacaagt
     5701 actacaggat agccatgatt acgccgtgcg taacgccaaa gaatattata aaagagttcg
     5761 tccattcgtt atttataaag acgcaacctg tacacctgat aaagatgaga aaatggctat
     5821 cactggctct tatccctctg gtcatgcatc ctttggttgg gcagtagcac tgatacttgc
     5881 ggagattaat cctcaacgta aagcggaaat acttcgacgt ggatatgagt ttggagaaag
     5941 tcgggtcatc tgcggtgcgc attggcaaag cgatgtagag gctgggcgtt taatgggagc
     6001 atcggttgtt gcagtacttc ataatacacc tgaatttacc aaaagcctta gcgaagccaa
     6061 aaaagagttt gaagaattaa atactcctac caatgaactg accccataaa gctggacagc
     6121 ctgtatcagg ctatggaggg cccatagaca aatctaccct atatgagcat aggaggagtc
     6181 tatgggcaca ccacgtttta ctcctgaatt taagggatta ctggaaaggc tgggacatat
     6241 ccctccggca gaagcagaaa aagcttatta tgctgccatc ggaaacgatg atctggcaac
     6301 ctgagttcac agataaaaca ttctctagga aactcggggc ggttccgttc accacatgca
     6361 atgtggtgtt gcaggggaac ggtctgcacc atcccctata tcgatcaata taacaaaaat
     6421 gacaacttca gattcagggc acaacctaaa tatattttag gtcacctctc aaatcgtttg
     6481 cctgataccg ctccattttt taataaaaaa tcaatcattt ttgaatcttt gcttttcatt
     6541 gcattgtcaa gcatagtgtc cccactattt gctttttgaa attttttgtt tacatcaacc
     6601 aatccattat tgataaaata ttctagcact ttatagttgg caccatcttt actaagtaag
     6661 tattcaacat catacagatt acaatatata ttgttgttca ttttctccat ctctgcgata
     6721 tcttgttttg taaaattaaa tttagatagt aaataaagtg ctatatcagc ttttttatcg
     6781 cctaaagaat aagcaagggc ctgatgagcc atttttttat cagtcacttt attgttaatc
     6841 tcttcataat tagatgcttt aatcgcatcc tccaaagata tgtctctgag ttttattttt
     6901 t
//
LOCUS       DAJTYF010000166         6802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTYF010000166 DAJTYF010000000
VERSION     DAJTYF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.167"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            94..1518
                     /gene="sbcB"
                     /locus_tag="OO590_003780"
     CDS             94..1518
                     /gene="sbcB"
                     /locus_tag="OO590_003780"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6638961.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1561..1788)
                     /locus_tag="OO590_003781"
     CDS             complement(1561..1788)
                     /locus_tag="OO590_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6638962.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1802..2860)
                     /gene="yeeE"
                     /locus_tag="OO590_003782"
     CDS             complement(1802..2860)
                     /gene="yeeE"
                     /locus_tag="OO590_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6638963.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALILAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3039..4397)
                     /gene="plaP"
                     /locus_tag="OO590_003783"
     CDS             complement(3039..4397)
                     /gene="plaP"
                     /locus_tag="OO590_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6638964.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4387..4467)
                     /gene="yoeI"
                     /locus_tag="OO590_003784"
     CDS             complement(4387..4467)
                     /gene="yoeI"
                     /locus_tag="OO590_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6638965.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(4665..5594)
                     /locus_tag="OO590_003785"
     CDS             complement(4665..5594)
                     /locus_tag="OO590_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6638966.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(5640..6464)
                     /locus_tag="OO590_003786"
     CDS             complement(5640..6464)
                     /locus_tag="OO590_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6638967.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6547..6801)
                     /gene="yoeB"
                     /locus_tag="OO590_003787"
     CDS             complement(6547..6801)
                     /gene="yoeB"
                     /locus_tag="OO590_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6638968.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1839 a         1781 c         1595 g         1587 t
ORIGIN      
        1 tatttgacgt tcgtagacat actcaggtga aaaaagggtt gcttttcgag ttataacaga
       61 cagttaatta actacagtgg acaatgctag acaatgacag acacagataa acaacctacc
      121 ttcctctttc acgattacga aacctttggc acgcaccccg cgttagatcg ccctgcacag
      181 ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg gcgaacccga agtcttttac
      241 tgcaagcccg cggatgacta tttaccccgg cctggagcag tattaattac cggtattacc
      301 ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt ttgccgcccg tattcactcg
      361 ctttttaccg taccgaagac ctgtattctg ggctacaaca atgtgcgttt cgacgacgaa
      421 gtcacacgca acgtttttta tcgtaatttc tacgatcctt acgcctggag ctggcagcat
      481 gataactcgc gctgggattt actggatgtt atgcgtgcct gttatgccct gcgcccggaa
      541 ggaataaact ggcctgaaaa tgatgacggt ctaccgagct ttcgccttga gcatttaacc
      601 aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga tggctgatgt gtacgccact
      661 attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc tgtttgatta tctctttacc
      721 catcgtaata aacacaaact gatggcgttg attgatgttc cgcagatgaa acccctggct
      781 cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca gctgggtggc accgctggcg
      841 tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt tggcaggaga catttcgcca
      901 ttactggagc tggatatcga tacattgcgc gagcgtttat ataccgcaaa agccgatctt
      961 ggcgataacg ccgccgttcc ggttaagctg gtgcatatca ataaatgtcc ggtactggcc
     1021 caggcgaata cgctacgccc ggaagatgcc gaccgactgg gaattaatcg tcagcattgc
     1081 ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc gtgaaaaagt ggtagcgata
     1141 ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg atgcacagct ttataatggc
     1201 tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc tggaaaccga gccgcgtaat
     1261 ttaccggcac tggatatcac ttttgttgat aaacgaattg aaaagttgtt gttcaattat
     1321 cgggcacgca acttcccggg gacgctggat tatgccgagc agcaacgctg gcaggagcac
     1381 cgccaccagg tcttcacgcc agagtttttg cagggttatg ctgatgaatt gcagatgctg
     1441 gtacaacaat atgccgatga caaagagaaa gtggcgctgt taaaagcact ttggcagtac
     1501 gcggaagaga tcgtctaatt tgaagccgcc gccgctggta cggcggagga caatgttgag
     1561 tcaggctttt tgaacggtga tgctccacgc tgcatcgcca atctgctgat aatcggtgat
     1621 ggcatgtcct tcttctgccg cccactgtgg gattgcttca gttgcctggg tacaatcaaa
     1681 ctcaattacc aactcatcgc cgctggccat ttctgccagt gctgcttttg cttctatgag
     1741 tggaaacgga cagacctgcg tcaccacatc cagcttttta atcgccatct ttacacctta
     1801 cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg cggtcgaaca tacacaagcc
     1861 aggacgcagt ccagactccg agaatcataa atacaaggcc aatccagccc tgccaggtca
     1921 tcattgcagt cataaccagt ccattaccga tagagcaacc acctgcaata ctggcaccga
     1981 agcccatcag tacaccacct aacccgctac gtaatgttgt ttgtgcatca gctgcgcgaa
     2041 cgcggaactc acggctcgct ttggctgcaa taaatgcccc cacgaagatc cctaacacca
     2101 ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac cagaaattgc agaatattgg
     2161 ccgttgggga agtgactcct aacccaaaca tgcgtccggt tgcttcactc aggggccagg
     2221 ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa gggatgccag cgtttttcaa
     2281 acagaatatg agcgatcccg gttcggcgcg gcggtaaggt cgcgactttg agttttggct
     2341 tcttcaactc tttcatcacc acccagagcg ttatcaccag caaaacggta accaacggcc
     2401 acacagacaa attaaaagtc tcagcaatag agttatgttc agtagtgtag tgctgcaagg
     2461 tttgatttaa accactggcg tgtggagaac gcatcaccgc actcatcacc atataagtga
     2521 aaagcgcgat ccaactgccg atcaatccct cacccgcgcg ataccaggtc ccggtggcac
     2581 atccacccgc cagaacaatt cccagcccga aaagataccc acctataaca gtaccaagcc
     2641 acgggaacgc cccggcttcg taagtcagta ggcccgccag aattaacgca aaaaccccca
     2701 cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat gcgattattt ttcgcgatat
     2761 acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg ctgcatgaca aatcccagca
     2821 gagcaccaca aattagcccg cttaatatca ttgaaaacat aaattaatta accagatgaa
     2881 tgttaatggg gaaatttttc atgactggtg gaatgcagac caataaccaa attctctaaa
     2941 ttagaacaaa tggttattaa tgaggatttc gataaaaaag aataaaaaaa ccggagccaa
     3001 atgttcattc gactccggtt tattattaga aaagatgatt atgctacgtc ttcgtactgc
     3061 ggaaccggat tacggaagct cttggtgacg caagccaggt aaatcagacc gatagcagcc
     3121 cagatcagac ccagaaccat tgagctttct tccaggttaa cccacagcgc accaacggtc
     3181 agcgcaccac acatcggcag gaacagatac tggaagtgat ctttcagcgt cttgttacgc
     3241 ttctcacgga tccagaactg cgagatgacc gacaggttaa caaaggtgaa cgccaccagc
     3301 gcaccaaagt taatcagcgc cgttgccatt accaggtcga agttgattgc cagcagggca
     3361 atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc atttcgggtg tacataaccg
     3421 aagaagcttt tcgggaacac gccgtcacga cccattacgt acatcagacg cgctacgcct
     3481 gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg agaagatcag cgcgccaacc
     3541 tggaacgctt tacccgcaac gtacagcatg atttcaggct gtgacgcatc cggatcttta
     3601 aagcgagaga tatccgggaa gtacagctgc aggaagtaag ttgcaaagat gaagatcatg
     3661 ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca cgcgctctgc atctttggtt
     3721 tcttccgaca gattgctgat gccgtcaaag ccggtaaagg agaagcacag gattgtcgcc
     3781 ccggtaatca tcgggataac atgcgcatcg ccagaccaga acggacgcgt gctcgccagc
     3841 gtaccagcgc cttcaccttc aaatacgcca taaacaacca tgcccagaat caccgcgatc
     3901 agcactacct gcaacacgac gattacagtg ttgaagttcg ctacggattt cagactacgc
     3961 aggttaaagg cggtcataaa ggccaccagc gccaccacaa acatccacga tggaatggaa
     4021 ggcaccagag cttcaaaata gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga
     4081 tagtcgagca gagaagacca acccaccata aagccgacag tcgggctaat ggatttctgg
     4141 gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa ccagcttccc gtagctcaga
     4201 gccgtaaaca ggatcgcaat caatgcgaac gcataggctg tcggcacatg accatccgtg
     4261 aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg gctgcatata ggctagaccc
     4321 atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga
     4381 gtaacgttat gcgacatggt tattctcctt tacggtgata accgtcgcgt aagcaaaaaa
     4441 tttgccccat ttttttggat tcctcagcga caacaactgt cgatttttag taaatatcta
     4501 tccggtacga agcccggcct cttggtatga atgattggtt tgaagcaaaa aataaccgac
     4561 gctaattaaa cgtcggtttt tagtcatttt ttgacagcgg cgcattgtgc ctaaatgggg
     4621 gggaaatgac aagagaatga gaggcttgtc ataataattt ttctttaaat ggctgatttt
     4681 ccgtcatcag attttgcata aacaccggcg atcgcactat ttgctaaaat ttcatctcgc
     4741 caccagccgc tggcaagccc tgcggtctgt tcattccagc caatccagac aggttcaaag
     4801 agagtttggg cgaccacttt cttctcgatt aacgcgccac tatcgagaaa acgttgcgcc
     4861 agataccgtg gtaaatagcc gcagccaagg ccgctaattt gcagctccag tttggtttta
     4921 aaatcgaaaa cggtaatggc ttcttgttcg tcgagaagtt gtgaggccgt agaagcgcct
     4981 gcctgcgcag tgtcccccac cacaatcgcc cgatatcgtt taatgatacg acggtttaac
     5041 ggttcttctt cctgggctaa cggatgatga ggcgcgaccg caaagacttg ttcaagatcg
     5101 cccaaccgcg agaaaccaaa ctcactggaa gatggtggct catgcatcgc gccaacgata
     5161 atatccgctc gcccctgagt cagggcatcc caggaaccac cgagtacgcc attgataaac
     5221 ttcaggcgcg tcacgctatg atgttgataa aaagcttcaa tgagaggcgc aagcagagaa
     5281 aaaggaaacg tgtcgtccac gccaatcacc agctcgtttt cccagccttc atgcagtttg
     5341 atcgcctgct tttccagttc tcgcacggta tgcagaactt cccgcccttt ctctaataac
     5401 atttttccgg tgcgggtgaa tttagcgcgg tgtccgctac gatcaagcaa ttgaatattt
     5461 aggtcgcttt ccagcttgtg aacggtgtaa ctcagagcgg acggcgtctt gtagagtttg
     5521 gctgacgccg ccgcaaaact gccctctttt tctatggcat cgaggatcat caacacatcc
     5581 agcagtggtt tcatacacgc ccccttgcag tgcatgcggc gtcctgctgg cgtgatcgtt
     5641 tactccagcg gcattaccag cggatcggga tactggtatt caaatcccag ttcattacaa
     5701 atccgactgc catcaataat cttacctttg ccgctgtcca gactatttct gaactgcgga
     5761 ggttgcagcc ccagtaaacg ggccatctgc ggatagaaaa cattacgcgc agggtgagct
     5821 ggcgcacata tattatagat gtgtccgcct ttaggtgcct gtaacaacag agtgatagcg
     5881 ccaataacat cttctaaatg gactaaatta acaccatgtt caccatcagg ggcggttttt
     5941 ccggcaaaga agcgtcccgg atgacgtccc ggtccgacca ggcccgcaag acgcagaata
     6001 tcgaccgaag taccgggtaa attgtgcagc cagtcttcga gttcttctaa tactcgtcca
     6061 ctgttggtta ccggattacg cggggtagtt tctttaaccg tgccttgcgc gtcgccatag
     6121 acagatgttg agctggtaaa aataattcgg ggaatacgat gggccagcgc gctatccact
     6181 aactcttgta ccgcttgtaa atagaactca tcgccggggc cgctacgacg tgccggaagc
     6241 gtaatgacca gcgcatcggc atccataagg gcatccagat cgtcagaatc gcaaactaac
     6301 tcaggctcca tgcgaagcag atagctatca atgccactca ttcgggccgc ttcgacacca
     6361 tcttgtgtgg ttttactccc ggtgacttgc cagcctcgcg ctgaaagtga catcgccagc
     6421 ggcataccta accaccctaa cccgacaatt gcgacctttt tcatccgtta tctcctgata
     6481 caccttagat ctataaggct acgctagcgt atcaaaactg acaattcatt ctatgaatga
     6541 atctgttcaa taatgataac gacatgctgc aatgagcagt gaatcatcag taaccgcgta
     6601 taccagacgg tgctcctctg taatgcgtcg ggaccagaaa cctgacaaat tatgtttcag
     6661 gggttctggt ttccccttac cttcaaatgg tgttctgcgg gtatctttga taagttcatt
     6721 gatcttttta acaattcgct tatctgtttc ctgccagtac agataatcat cccatgattc
     6781 ctcagaccag attagtttca ct
//
LOCUS       DAJTYF010000167         6741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTYF010000167 DAJTYF010000000
VERSION     DAJTYF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.168"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..441
                     /locus_tag="OO590_003788"
     CDS             <1..441
                     /locus_tag="OO590_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6638969.1"
                     /translation="VEQIAELESELSQSINADDTAQRIMTIPSVGPITASLLSSQLGD
                     GKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLLVQCARSFMIQLERQ
                     HGKLAEWVREQLNKKHSNVVACALANKLARIAWAITTQQKEYQA"
     gene            complement(744..1514)
                     /locus_tag="OO590_003789"
     CDS             complement(744..1514)
                     /locus_tag="OO590_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638970.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1559..1993)
                     /locus_tag="OO590_003790"
                     /pseudo
     CDS             complement(1559..1993)
                     /locus_tag="OO590_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2005..2226)
                     /locus_tag="OO590_003791"
     CDS             complement(2005..2226)
                     /locus_tag="OO590_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638971.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2227..2910)
                     /locus_tag="OO590_003792"
     CDS             complement(2227..2910)
                     /locus_tag="OO590_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6638972.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2974..3285)
                     /locus_tag="OO590_003793"
     CDS             complement(2974..3285)
                     /locus_tag="OO590_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638973.1"
                     /translation="MKYDGDGRATARFFSDKGCRRVPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3282..4184)
                     /locus_tag="OO590_003794"
     CDS             complement(3282..4184)
                     /locus_tag="OO590_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6638974.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4624..5583
                     /locus_tag="OO590_003795"
     CDS             4624..5583
                     /locus_tag="OO590_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6638975.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5583..5978
                     /locus_tag="OO590_003796"
     CDS             5583..5978
                     /locus_tag="OO590_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6638976.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6161..6541
                     /locus_tag="OO590_003797"
     CDS             6161..6541
                     /locus_tag="OO590_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR6638977.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6587..>6741
                     /locus_tag="OO590_003798"
     CDS             6587..>6741
                     /locus_tag="OO590_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6638978.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWR"
BASE COUNT         1831 a         1749 c         1641 g         1520 t
ORIGIN      
        1 gttgagcaaa tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc
       61 gctcagcgca ttatgacaat tcctagtgtt gggccgatta ctgccagctt actatcgtcg
      121 caacttggtg acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc
      181 gttccccggc agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt
      241 gataaaaatt tacgccggtt actggttcag tgtgcccgct cattcatgat acagcttgaa
      301 cgccagcatg gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat
      361 gtggttgcct gcgctctggc aaataaactg gcccgaattg cctgggcaat cactactcag
      421 caaaaagaat atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa
      481 caataagata caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc
      541 aaatcgacct gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt
      601 taggattatc aggtgcggaa ctcatcgagg cgcgggcatc ccttgcccac agagacgccg
      661 gatagattta agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc
      721 atagattttc cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg
      781 cggatttcgg cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg
      841 cggggcggtt catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc
      901 tcaatacaat cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg
      961 atggcatcga gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg
     1021 ctttccttca cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt
     1081 gtggtcagcg ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat
     1141 actgcatcgc gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcacag
     1201 gcatacggga aagacacctg cgttccgtcc gccggaagta gcggcagcga ctgccactga
     1261 ctgccggaca gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg
     1321 tgcggcggca gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc
     1381 agccgcacgt aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc
     1441 tcttccggcc agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc
     1501 agcgtttcat tcataattca tgaactccct gtatcgccgc ccgcaggcgg cgataacatc
     1561 agtcgttttc gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc
     1621 ggcgggtaac gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc
     1681 acagtgcctg ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct
     1741 ggcggttttc gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact
     1801 caatccgcta gtcttccggg atgccctcca ggtgcgccag gacggtcctg atttcatgat
     1861 cggacaggga aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa
     1921 ccagcatcag cggcgtgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc
     1981 atgttccgta catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc
     2041 ggtatatgcc tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc
     2101 cggcagggtg tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag
     2161 gtcgcacatg gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata
     2221 gttcatttac gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc
     2281 ccgctgtacg gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat
     2341 accgatatac cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa
     2401 cgggtccagc actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt
     2461 aacgggcttt tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg
     2521 cagcgggttt tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg
     2581 atagcccaca tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac
     2641 gctgaatccc gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa
     2701 gctcaccatc agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag
     2761 ccattcatca gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag
     2821 atatggcgga tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc
     2881 gatgcagtta ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc
     2941 tgtggtgccc gttccccgct gcctgtgggc tgttcatcgc gtccggtaac gggtcaggcc
     3001 gaccgtggaa taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct
     3061 tgaagggcgt tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acgccagccg
     3121 ggagcggtaa aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg
     3181 tttatgccgc gccgcgtcag ccggcgcagt aaaaagagga acacggcgac atcctttgtc
     3241 ggaaaagaaa cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc
     3301 acattgtcga ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg
     3361 gaagaccatt caagttcccc ccacagcaca tcgacgagct ctccacgctc atacaactgc
     3421 cagccacaga aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg
     3481 cggcttaatt ctgcaataac gtcgctttcc ggctgacacc acggcgtgtc gaaatccacc
     3541 tgaatgaagt tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg
     3601 ccataccgtt ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta
     3661 aagccgttca cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg
     3721 gttttttccg gcactacgtt gtcacacaga cggttccacc acacagatac atcctcgtta
     3781 ccccatatgt cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg
     3841 gcatcatccg gtaatccttc ccagcgacgc tggccagtac cgctctgtaa ccagagttca
     3901 tgcagcatca ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tgtcagccag
     3961 cgggtgaacg cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg
     4021 cactgttcag agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac
     4081 agctgaatac cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt
     4141 cgtttaatct cagcgatctg tgccggttca ccaggaaaat acatacgatt cgagcaccag
     4201 tttggcatgg gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac
     4261 cgggcgaccg catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg
     4321 acgaaacgtg accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc
     4381 ggttaacgtg ccggcacggc ctggttaagt agtatccgtc tcagttcgtg gcgcaaaatg
     4441 agaaaccaat aagaactcaa aatgaaccac aacaagaaca taaagagact ctaaaagagc
     4501 aacaccgctt gacatagact caaaatgagt cacaataaga aacaataaaa aactcaaaaa
     4561 gatgcaccga tataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa
     4621 agaatgctaa aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa
     4681 gatggcgagg taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga
     4741 ctgtttaatg ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat
     4801 ttgggaagca ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat
     4861 ctgcttgcca tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg
     4921 acagtaacat tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga
     4981 atagaaagaa aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt
     5041 aaaattaaaa aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa
     5101 aaagataaag taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg
     5161 gattgtggtt taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa
     5221 atcgggacat cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca
     5281 tgtaattatt ttattgctga tgagttaata aaaaacagac acgataatga atatctccag
     5341 acattaataa atgatgtagc tgaaataaaa aacatttctc atgtaataga cagagaagtg
     5401 aaatctctgg cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc
     5461 tatctcactg gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat
     5521 ctgaaagtga acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg
     5581 taatggaaag cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac
     5641 tttatccgca ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg
     5701 gagatttcta cagacaatcc cttatttgcg gagcagctct ttattctgtt gacccccgac
     5761 tgttaacgct aatcagcggt tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac
     5821 ttatagaaca aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca
     5881 tcatagaggc atcttcagaa aacaaatcag agagcatcac ttcaaaagat gatttcgaag
     5941 aacaaaccag acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc
     6001 aattacctgc tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta
     6061 tcataaaaaa tgcaacagac aggaatgcaa taattttgca ttatctcgtt gcttgtaatg
     6121 caaatatgca ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa
     6181 cgcacgtatt gatgatgata taaataacca agcagatgaa gtgctgaagc tgatgaatat
     6241 cagtcaaact caggctattg cagcttttta tcagtatatt actgaacaaa agaaactgcc
     6301 atttgtgatc acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat
     6361 gttagctgaa gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg
     6421 gattggaaaa gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc
     6481 aaaagagaaa ataggtcttc tttcagataa ccgggatgct gaactaggct gtgtccctta
     6541 atcatctgag ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga
     6601 ccttcccgat gaggcatgga ccatcatcaa gcctctgtta cctcctgaac ccgccacacc
     6661 acgggccgga cgcccatggg ctgagcaccg taaaatcatc aatggcatgt tctgggtgtt
     6721 atgttccggt gcaccatggc g
//
LOCUS       DAJTYF010000168         6715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTYF010000168 DAJTYF010000000
VERSION     DAJTYF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.169"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..766
                     /locus_tag="OO590_003799"
     CDS             <1..766
                     /locus_tag="OO590_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6638979.1"
                     /translation="NDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLT
                     YDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLE
                     IDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVK
                     AEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQ
                     VTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            777..1850
                     /locus_tag="OO590_003800"
     CDS             777..1850
                     /locus_tag="OO590_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6638980.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSSLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2098..3138
                     /gene="pstS"
                     /locus_tag="OO590_003801"
     CDS             2098..3138
                     /gene="pstS"
                     /locus_tag="OO590_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6638981.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLEKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            3225..4184
                     /gene="pstC"
                     /locus_tag="OO590_003802"
     CDS             3225..4184
                     /gene="pstC"
                     /locus_tag="OO590_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6638982.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4184..5074
                     /gene="pstA"
                     /locus_tag="OO590_003803"
     CDS             4184..5074
                     /gene="pstA"
                     /locus_tag="OO590_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6638983.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5166..5939
                     /gene="pstB"
                     /locus_tag="OO590_003804"
     CDS             5166..5939
                     /gene="pstB"
                     /locus_tag="OO590_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6638984.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPELRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5954..6679
                     /gene="phoU"
                     /locus_tag="OO590_003805"
     CDS             5954..6679
                     /gene="phoU"
                     /locus_tag="OO590_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6638985.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
BASE COUNT         1697 a         1572 c         1746 g         1700 t
ORIGIN      
        1 aaacgataat tccggtagct cacatcaact cggactcagc ggtacggcgc tggaaagaaa
       61 taacctttca tggaatttaa tgcagtccta taacagtcat gatgatgagg ttggcggtaa
      121 tatgtccctg acctatgatg gctcttatgg cacggtgaac ggcagctata actacagcca
      181 aaattcccag aggctgaatt atggtatcag agggggaatt ctggcacaca gcgaaggggt
      241 aacgttaagt caggagttag gtgaaactat tgctcttgtt aaagcacctg gggccgccgg
      301 gttagaaata gataatatgc gcggtgctgc gacggactgg cgcggctata cggtcaagac
      361 acagctaaac ccttatgatg aaaatcgggt agcaatcagc gataactatt tctcgaagtc
      421 gaatatagaa cttgataata ccgtcgttac gatggttccc acgcgtggtg cagtggttaa
      481 agcggagttt gtgactcatg tgggttatcg cgttctcttc agagtgttaa atgcaaatgg
      541 taaaccggta ccttttggag ccattgctgc gatacaagat gcaagtttgg cagattcagg
      601 aattgtcggt gaccgtggcg aactttatct ttctggtcta ccagaaaaag gacaggttac
      661 gttatcctgg ggagaaaacg cctcaacaaa atgcatcttc aattattcac tttcgacacc
      721 agaaagtgag agcggattaa ttgaacaggg tgtgacatgt cattaaaagg atttttatga
      781 acaagtatat caaacagtgg tgcttttctg tgtttatgct ctcgttaagt agtgtagcac
      841 ttgcagctcc taaaggtatc tgtacccctg ataatggcgt atttcacagt tcgcttgatt
      901 tctcgggata tcttattacc gcgaacgaaa ataaggttgg aacgacattt aacaccactg
      961 taactaatgg atcctcatat cttggacgct gtcattgtga tactggtaac gtaggagaat
     1021 tcccctatat ctattatacg tcaaaaataa accaggcatt aacttatgct ggggttcact
     1081 ctaatattaa ttattatgat cttaatccga accttgatgt tgggattgct atagatattc
     1141 ttggggttgg gtatgtgaat gcaccttttg aatatcatgc taacaacccg tctggtaata
     1201 caaagtacaa ttgcaaccgc attgaacctt taagtatatc cagtggggct aaagcgatag
     1261 tatattttta tatcaagaag acatttgcag gaaaattgat tattcctgaa acaaagatag
     1321 tgacattgta tggaacaatt agccgtgaca ccccggtgga ttactcacag ccgatggctg
     1381 atgtttatat tcggggcgat attacagctc cgcaaagttg cgaaataaat aatttaaagc
     1441 cagtttgttt tgattttaaa gaaataccag ctgcagattt ttcatctgtt gttggaagtg
     1501 cggtaacaac acataaaatt actaaaacag tcactattga gtgtgaaaac ctagggatac
     1561 tgaataccga tgatatcagt acgtcttttt atgctaccga acccaatacg gacaattcaa
     1621 tggtcgtaac ttcaaactct aacgtgggga taaaaattta cgacaaaaat aataaggaaa
     1681 tcaaagtgaa cggtggtgag ttgccaacag acatggataa atcaacagtg tatggtgaaa
     1741 aatcaggtag tgtaactttt tcagctgctc ctgcgagcct tactggagct cgcccagccc
     1801 cggggcaatt tactgcaaca gcgacgataa ctgttgagat tgtgcgctaa taggtctgac
     1861 aacgattgtt catcacatcg ttgtcatctc acccatttct ttatcacttt taaatgcatc
     1921 atgacaaaaa gatggcatgt gggattttca tcttcacatt taaaatcagt aagttatttg
     1981 gtaattcctt tctgtcataa aaatgtcata ttccttatat ataactgtca cctgtttgtc
     2041 ctattttgct tctcgtagcc aacaaacaat gctttatgaa tcctcccagg agacattatg
     2101 aaagttatgc gtaccaccgt cgcaactgtt gtcgccgcga ccttatcgat gagtgctttc
     2161 tctgtgtttg cagaagcaag cctgacaggt gcaggtgcaa ccttccctgc gccggtgtat
     2221 gccaaatggg ctgacactta ccagaaagaa accggtaata aagttaacta ccagggtatc
     2281 ggttcttccg gtggcgtaaa acagattatc gctaataccg ttgattttgg tgcctctgac
     2341 gcgccgctgt ctgacgaaaa actggctcag gaaggtctgt tccagttccc gaccgtgatt
     2401 ggcggcgtgg tgctggcggt taacattcca gggctgaagt ctggcgaact ggtgctggat
     2461 ggtaaaaccc tcggcgacat ctacctgggc aaaatcaaga agtgggatga tgaagccatc
     2521 gccaaactga atccgggtct gaaactgcct tcacaaaaca ttgctgtagt acgccgcgca
     2581 gatggctccg ggacttcctt cgtcttcacc agctacctgg agaaagtgaa cgaagagtgg
     2641 aaaaacaacg ttggtactgg ctctaccgta aaatggccga tcggtctggg cggtaaaggt
     2701 aacgacggta tcgccgcgtt cgttcagcgt ctgccgggtg caattggtta tgttgaatat
     2761 gcttacgcga agcagaacaa cctggcgtac accaaactga tctccgctga tggtaaaccg
     2821 gttagtccga ccgaagaaaa cttcgctaat gcagcaaaag gtgcagactg gagcaaaacc
     2881 ttcgctcagg atctgaccaa ccagaaaggc gaagatgcat ggcctattac ctctaccacg
     2941 ttcattctga tccacaaaga tcagaagaaa ccagaacaag gcacagaggt gctgaaattc
     3001 ttcgactggg cgtacaaaac cggggctaaa caggcgaacg acctggatta cgctagcctg
     3061 ccggatagtg tagttgaaca ggttcgcgct gcgtggaaga ccaatattaa agacagtagc
     3121 ggtaagccgc tgtactaata aaactccagg ccgggtacgg tgttttacgc cgcatccggc
     3181 attacaaaat gactttgtaa acgcgtttaa ctgaagagta acttatggct gcaaccaagc
     3241 ccgcttttaa cccaccgggt aaaaagggcg acataatttt cagcgtgctg gtaaaactgg
     3301 cggcgctgat tgtgctattg atgttgggtg gcattattgt ctctctgatc atctcctcct
     3361 ggccgagcat tcagaaattt ggtctggctt tcctatggac caaagagtgg gatgcaccga
     3421 acgatatcta cggggcgctg gtgccgatct acggtacgtt ggtgacttcg tttatcgcgc
     3481 tgctgatcgc cgtcccggtg agtttcggta tcgccctgtt cctgactgag cttgcgcctg
     3541 gctggctgaa acgcccgctg ggtatcgcca ttgagctgct ggcagccatt ccaagtatcg
     3601 tttacggcat gtggggcctg tttatctttg cgccgctgtt cgccgtttac tttcaggagc
     3661 cggtcggcaa tatcatgtcg aatatcccga ttgttggcgc gctgttctct ggccccgcat
     3721 ttggtatcgg tatcctcgcg gcaggcgtga tcctcgccat catgattatt ccgtacattg
     3781 cggcggtaat gcgtgatgtg ttcgaacaaa ccccggtgat gatgaaagag tcggcctacg
     3841 gtattggctg caccacctgg gaagttatct ggcgtatcgt tcttccgttc accaaaaatg
     3901 gtgttatcgg cggcatcatg ctggggctgg gccgcgcgct cggtgaaacc atggcggtga
     3961 cctttatcat cggtaacacc taccagctcg acagcgcctc gctgtacatg ccgggcaata
     4021 gtatcacctc tgcgctggcg aacgaatttg cggaagcgga atccggtctg cacgttgccg
     4081 cactgatgga actgggcctg atcctgtttg tgattacctt catcgtcctc gccgcatcga
     4141 agtttatgat tatgcgcctg gctaagaatg agggggcacg ctaatggcta tggttgaaat
     4201 gcaaaccact gcggcgctgg ctgaatctcg ccgcaaaatg caggcgcgtc gccgcctcaa
     4261 aaaccgtatt gcgctgacgc tctcgatggc gacgatggcc ttcggcctgt tctggctgat
     4321 ctggatttta atgtccacca tcactcgcgg tatcgacggt atgtcgctgg cgctgttcac
     4381 tgaaatgacg ccgccgccca atacggaagg tggtggtctg gcgaacgccc tggcgggtag
     4441 cgggctgtta attttgtggg ccacggtatt cggtacgccg ctgggcatta tggcggggat
     4501 ttatctggcg gaatatggtc gaaaatcctg gctggcagaa gtgattcgct tcattaacga
     4561 cattctgctc tccgcgccgt cgattgtggt tggtctgttt gtctacacca tcgtggtggc
     4621 acagatggag cacttctccg gctgggcggg cgtaattgcg ctggcgctgt tgcaggtgcc
     4681 gatcgttatc cgcaccaccg agaacatgct gaaactggtg ccggacagcc tgcgtgaagc
     4741 ggcttatgcg ctgggtacac cgaagtggaa gatgatctcc gcgattacgc tgaaagcgtc
     4801 ggtgtccggg attatgaccg gtatcctgct ggcgattgcc cgtattgccg gtgaaaccgc
     4861 gccgctgctg tttaccgcgc tctccaacca gttctggagc acggacatga tgcagccgat
     4921 cgccaacctg ccggtgacga tctttaagtt tgcgatgagc ccgtttgcgg aatggcaaca
     4981 actggcctgg gccggggtat tgatcattac cctgtgcgtc ctgctgctga acattctggc
     5041 gcgcgttgtt tttgcgaaga ataaacacgg ttaacacttt gccggatgcg gcgtgaacgc
     5101 cttacccggc cctacggtaa gcctgattag cgaaaagcat caggtacgat gaggaaaaga
     5161 ttgcaatgag tatggttgaa actgccccga gtaaaattca ggttcgtaat ttgaacttct
     5221 actacggcaa attccatgcc ctgaaaaaca tcaacctgga tatcgccaaa aaccaggtta
     5281 cggcgtttat cgggccgtcc ggctgcggta aatcgacgct gctgcgtacc ttcaacaaaa
     5341 tgtttgaact gtacccggag ctgcgtgcgg aaggtgaaat tctgcttgat ggcgacaaca
     5401 tcctgaccaa ctctcaggat atcgccctgc tacgtgcgaa agtgggcatg gtgttccaga
     5461 aaccgacgcc gttcccgatg tccatctacg acaacatcgc ctttggcgtt cgtctgtttg
     5521 agaagctctc ccgtgccgac atggacgagc gcgtgcagtg ggcattgacc aaagccgcat
     5581 tgtggaacga aaccaaagat aaattgcacc agagcggtta ctctctctct ggtggtcagc
     5641 aacagcgtct gtgtattgcg cgtggtatcg ccattcgccc ggaagtgctg ctgctcgacg
     5701 aaccgtgttc ggcgctcgac cccatctcta ccgggcgtat tgaagagctg atcaccgaac
     5761 tgaagcagga ttacaccgtg gtgatcgtta cccacaacat gcagcaggct gcgcgttgtt
     5821 ccgaccacac ggcatttatg tacctgggcg aattgattga gttcagcaac acggacgatc
     5881 tgttcaccaa gccagcgaag aaacaaacag aagactacat caccggtcgt tacggttgat
     5941 tcaggagtgc gttatggaca gtctcaatct taataaacat atttccggcc agttcaacgc
     6001 cgaactggaa agtatccgca cgcaggtgat gaccatgggc ggcatggtgg agcagcagct
     6061 ttctgatgca atcaccgcga tgcataacca ggacagcgat ctggcgaagc gcgtgatcga
     6121 aggcgacaag aacgtcaaca tgatggaagt ggcgatcgat gaagcctgcg tgcgcattat
     6181 cgccaaacgt cagccgacgg cgagcgacct gcgactggtt atggtgatca gtaaaaccat
     6241 tgccgagctg gagcgtattg gcgacgtggc ggacaaaatc tgccgtactg cgctggagaa
     6301 attctcccag cagcatcagc cgttgctggt aagtctggag tcgctgggcc gtcataccat
     6361 ccagatgctg cacgacgtgc tggacgcgtt cgcgcggatg gacattgacg aagcggtacg
     6421 tatttatcgt gaagataaaa aagtcgatca ggaatacgaa ggtattgttc gtcaactgat
     6481 gacctacatg atggaagatt cgcgcactat tccgagcgta cttaccgcgc tgttctgtgc
     6541 gcgttcaatc gaacgtatcg gcgaccgctg ccagaatatt tgtgagttta tcttctacta
     6601 cgtgaagggt caggatttcc gtcatgtagg cagtgatgag ctggataaac tgctggcggg
     6661 aaaagatagc gacaaataat tcaccagaca aatcccaata acttaattat tggga
//
LOCUS       DAJTYF010000169         6684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTYF010000169 DAJTYF010000000
VERSION     DAJTYF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.170"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..3427
                     /locus_tag="OO590_003806"
                     /pseudo
     CDS             1..3427
                     /locus_tag="OO590_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3437..3680
                     /locus_tag="OO590_003807"
                     /pseudo
     CDS             3437..3680
                     /locus_tag="OO590_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            5219..6684
                     /locus_tag="OO590_003808"
                     /pseudo
     CDS             5219..6684
                     /locus_tag="OO590_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1767 a         1754 c         1615 g         1548 t
ORIGIN      
        1 tctttcgtag cagaaacccg tgcggctgac aaaaatacag atgctgtttt accttggctg
       61 gcgaaagata ttgctgtcgt ttttccgccc gaagttgtac acaccacgtt aagtcatcgc
      121 cgctttcccg gagttcctgt tcagcaagca gacaaattga cccaattacg tcgcttagcc
      181 tgtagcgtgt cgcaacggga taataaaaca gccacatttg acttcagcac ctgttcttta
      241 gaatggcaaa acaccgtcgc ccaagcgatc agtcagatcg acggcctgaa aacaacacag
      301 ctaccatcac cagtaatggc tgtactcacg gcacttgaaa tgaaatgcac aagatataaa
      361 gtgcgtgagg atgttatgga tcagatcgtc caggaaggcg gtctggaata tgctactgat
      421 gtaataatac accttcaaca gattgatatt aaatgggatt atgcgaataa tgtcattatt
      481 attctgccgt ctggcattgc acctgactac ttggagcaat attccagatt tgaattacgc
      541 ctacgtaaac atttatcact ggcggaagag tctctctggc aaaaatgtgc acaaaaactt
      601 attgccgcaa ttccacatat tccagaatgg cggcaaccat taattgcttt gttattaccc
      661 gaaaaaccag aaattgcaca tgaaattgcc cagcgtctac tggggcaaaa aaaattaccc
      721 tcgcttgagt ggttaaaaat agtggcgact gatgagcaca ttcttgcctc attagaaaaa
      781 tatcacgagc catatgccat ttttgatgat tactattgtg gtgcgatatg gtcagccacc
      841 gtattacagg agcaaggtgt tgcagccctg ccccgatttg ctccctatgc cgcaagtgac
      901 tactgcgccg atgtgttgcg tcatatcaat catccgttcg cattaacact gcttatacgt
      961 gtagccgggc atactaaacg ctgtcacgat cgaatgacga aagcctgcgc tgcgttccca
     1021 cacgcagcac tggcagaaca ggtcattccc tggctttcga cacccgcagt tgcagtgctg
     1081 aaatcatgcc agcaccaact gacacagccc tcaaaccatg ccagcgccga tctactgcca
     1141 gccatagtag tctccccacc atggatttcg aaaaagaaaa aatcgccgat tccggtgctg
     1201 gatttagcgc cattaggcat cgagccaatc tgttatctga cagaagaaat cagtaatcaa
     1261 cttttggcga aatatatctg gtattcaaaa cacatcacgg ttagccatga agaaagtact
     1321 accaacctgt tggcaaggat gggttttcaa cgacggatcg ctggtacata tattaaagct
     1381 cccgaagcgg tagttgaggc atggctaaat gaagattatt caaccttact aagtgaattt
     1441 aaggtgtttc attcacctac cgggcattat tggcagttgg ggattttgac aacattgccg
     1501 ctggaggctg gagaaagcag taaaagcatg gaatgccctt actctatctc cacataccga
     1561 taccgaatac gccatgttac attttggact caaaggttta cctggtttag ttaactcaat
     1621 tacacgctat ccgcaggaag ccttgcccat cacgaattac ttcgcagcga gtgagctggc
     1681 tcctgccgtc gcccgtgcct tcaacaaact caaaacgcta cgcgaaaacg cccgcacctg
     1741 gctgttgaaa tacccggaac atgcccttac cggcctgctg cctgcggcgc tcggcaaagc
     1801 cggtgaagca caagataacg cccgcgctgc cttgcgtatg cttaccgaaa acggtcatca
     1861 gccattactg caagaaatcg cccggcgtta taaccagtcg gaagtaacag acgcggtgaa
     1921 cgctctgctt gcgctcgatc ccttagataa tcacccgaca aaaatcccca ctcttccgac
     1981 cttttatcag ccatcgatct ggacgcgccc ggtattaaaa gcaaacgccc aatcactgcc
     2041 agataacgcc ctcctccacc tcggcgaaat gctccgcttc cctcaggaag aggctctgta
     2101 tccgggatta ttgcaggtga aagacgcctg taccgctgac tctctggctg aatttgcctg
     2161 ggatctgttt accgcctggc agaccgctgg cgcgccgtcg aaagagagtt gggcgttcac
     2221 tgcgttaggc gttctcggta acgatgacac cgcccgcaaa ctgacgccat taatacgcgc
     2281 ctggcctggt gaatcccagc ataaacgcgc caccgttggg ttggatattc tcgccgctat
     2341 cggtagtgat atcgccctga tgcagcttaa cggcattgct cagaaactga aattcaaagc
     2401 attacaggag cgggcaaaag aaaagattgc cgacattgcc gagagccgcg aactcacggt
     2461 agcggagctt gaagatcggt tagcaccgga tctcggtctg gatgataacg gttcgttgct
     2521 gctggatttt ggcccacgcc agttcaccgt cagctttgat gaaaccttaa aaccgtttgt
     2581 gcgtgatgtt tccggcagcc gcctgaaaga cctgccaaaa ccaaacaaaa gcgatgatga
     2641 gtcacaggcg aacgatgctg ttaaccgcta caaattgctg aaaaaagatg cgcgtaccgt
     2701 cgccgcccag caggtagcaa ggcttgaatc cgccatgtgc ctgcgtcgcc gctggtcgcc
     2761 ggaaaacttc caactcttcc tggttgtgca tccgctggtt cgccacttaa cccgccgtct
     2821 gatttggggc gtttatagcg ccgacaacca gctactggcc tgtttccggg tagcggaaga
     2881 taacagctac agcaccgctg acgatgatct tttcaccctg ccggaaggcg atatctctat
     2941 cggcattcct cacgttctgg aaatatcacc gacggatgct gtcgcctttg gtcagctttt
     3001 tgccgactac gaactgctac caccgttccg tcagcttgac cgtaacagtt acgccctgac
     3061 agaagccgag cgcaacgcca gcgaactgac ccgctgggca ggcagaaaat gcccgagcgg
     3121 ccgggtcatg gggctggcaa ataaaggctg gataaagggc gaaccacagg atggaggctg
     3181 gatcggctgg atgatcaaac ctctcggtcg ctggtcgtta atcatggaaa tcgacgaagg
     3241 ttttgcggtg ggtatgtcac cagccgaact tagcgctgag cagctcttaa gcaagctgtg
     3301 gctatgggaa ggcaaagcgg aaagctatgg ctgggggagt aattcaacac aggaagcgca
     3361 gttctccgta ctcgatgcca tcaccgccag cgagctaatt aacgatattg aagccctgtt
     3421 tgaataagga aagagcatgg acaaggaatt accgtggctg gcggataacg cccaactgga
     3481 actgaaatat aaaaaaggca aaacaccact cagtcatcgc aattggccgg gcgaaccagt
     3541 gcctgttatc actgaaagca tcattcagac actgggtgat gaattgctac aaaaagctga
     3601 gaagaaaaaa aacattgtct ggcgttatga aaatttttca ccggagtggc agtccgccat
     3661 tacgcaggcc atcaacttga tcggcgaaca caaaccctca gtcccggccc gcacaatggc
     3721 ggcactagcc tttatcgcgc aaaatgacaa ccaatagttg ctcgacgaaa tcgtccaaca
     3781 agaggggctg gaatatgcga ctgaggtggt gattgcacgc cagtttattg cacggtgtta
     3841 tgagagtgat cctctggtag tcacattgca gtatcagaac gaggactatg gctatggtta
     3901 tcgctcagaa acctataacg aattcgatct ccgactgcgt aaacacctct ctctggcaga
     3961 ggaaagctgc tggcagcgtt gcgccgacaa actcattgcc gcactgccag gaataaccaa
     4021 agttcgccgc ccttttattg cgctgatcct ccccgaaaaa ccagaaatcg ccaatgagtt
     4081 ggtaagcctt gaatgcccgc ggactcattt tcattctaag gagtggttaa aagttgttgc
     4141 tactgacccc aaagcggtga gaaaactcga acgctactgg agccaggata tatttagcga
     4201 tcgagaagcc agctacatgt cgcatgaaaa ccacttcggc tacgcggcct gcgctgccct
     4261 tttgcgcgaa caaggactgg cagtcgttcc gcgcctcgcg atatatgccc ataaagaaga
     4321 ttgcggcagt ctgctggtac aaattaacca tccgcaagtc atccgcacct tgctgttagt
     4381 tgctgataaa aacaaaccca gcctgcaacg tgtggctaaa tacagtaaaa acttccccca
     4441 tgcgacactc gccgcactgg cagaactgct ggcgttaaaa gaaccaccag cccgcccaag
     4501 ttatccaatc atcgaagaca aaaagctgcc tgcacagcaa aaagctcgag atgaatactg
     4561 gcgtacgctg ttacaaacgc tgatggcatc gcagccacaa ctggcagcag aagtgatgcc
     4621 gtggttaagt actcaagccc gggcagtgct gaatagttat ttatcggcac cgcccaaacc
     4681 agttattgat agcaccgata acagccaaat gcctgaaatc ctcgtttcac caccatggcg
     4741 tgggagaaag aaaaagactt ccatacgtca cttttcgctg cctgatctta cgcttgcacc
     4801 gcaaacgtac aggtcgccga ataaacactg gacgccggaa cagcaaactg caattgacca
     4861 ttttagcgcc atgtcttttg acgaacgtct tgctacgaga ggagcggata tcttcttgcg
     4921 ggaattaggt tttgacgttc atagcctgga atttaataag cgtcttctta atggacagtt
     4981 ggatgccttt gatgctcagc aactgcaaga atacacatca ataggactat tgcgtactta
     5041 tcacgcgcat caggccaaat ctaaacagta ccgggaagac gccaccaatg cattactggc
     5101 acaagatagc attgcgttga tgggagggtg gcgaactttt cagcacggat tttactatgg
     5161 taatgaacag cgttggggga tttggaatct gtaccttatc gcgcagatgc cgcgcgaaat
     5221 ggcggtatcg tgctggcaac aaattgtcga ggctgatttt cattacaccg gggtggaata
     5281 tttgttaagt gtgctgggta ccgatgcttt gcctggatta aacaccgcct ttgcccgaca
     5341 tccaaaagag atatttccgc tgctgattca tttcggcgca acagaactgg ccctgcccat
     5401 tgcccgcgtc tggcaccttt ttgcgggcca gcgtaatctg gctcgccagt ggattttaca
     5461 atggccggaa catacggcta ctgcacttat ccctcttgtc ttcaccaaat ctagcgataa
     5521 aagcgaagct gcattacttg ccctgcgttt actttacgaa catgggcatg gcgaattgct
     5581 acaaactgta gccaatcgtt ggcagcgtac agatctatgg cctgccctgg aacaattact
     5641 taaacagagt ccaatagaga tttacccggc acgcattcca aaagcccctg atttctggca
     5701 tccgcaaatg tggtccaggc cgcgccttat cactaataat caacctgtta ccgatgacgc
     5761 tctggaaatt atcggcgaaa tgctgcgctt tacccagggg ggacgttttt atagcgggct
     5821 ggaacaactg aaaacgttct gccagccaca aacgctggca gcttttgcct gggatctctt
     5881 cactgcgtgg caacaagctg gtgcccccgc aaaagacaac tgggcatttc tggcgttaag
     5941 tctctttggt gacgaaagca cggcacggga tctaacgaca catatcctcg cctggccaca
     6001 agaaggcaaa tctgcccgtg cggtcagtgg cctgaacatc cttaccctga tgaataatga
     6061 tatggcgctg atgcagctgc atcatatatc gcaacgggcg aaatcccgcc ccttacgtga
     6121 taacgcggcg gaatttcttc aggtagtcgc agaaaatcgc gggctaagcc aggaagagtt
     6181 agcggacaga ttagtcccaa cactgggcct taatgatccg caggcgttga gttttgattt
     6241 tggtccccgg cagtttaccg ttcgcttcga tgaaaacctc aacccggtta tctttgatca
     6301 gcaaaacgtt cgccagaaaa gtgttccccg gttgcgcgcc gatgacgatc aactgaaagc
     6361 gcccgaggca ctggcccgac taaaagggtt aaaaaaagat gctactcagg tgagcaaaaa
     6421 cctgctcccg cgtcttgaag ctgccctacg taccacccga cgctggtcgc tggcagattt
     6481 tcattctctg tttgttaatc atccctttac ccgtctggtt acccagcgat taatatgggg
     6541 ggttatccgg caaatgaacc gcgtcgttta ctcaacgcct ttcgtgtggc cgcagagggg
     6601 gagttctgca atgcgcaaga tgagccaatt gacctgcctg cggacgctct gattggcatt
     6661 gcccacccgt tagaaatgac agca
//
LOCUS       DAJTYF010000170         6674 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTYF010000170 DAJTYF010000000
VERSION     DAJTYF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.171"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..171)
                     /locus_tag="OO590_003809"
     CDS             complement(<1..171)
                     /locus_tag="OO590_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6638986.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTEVY"
     gene            complement(321..482)
                     /locus_tag="OO590_003810"
     CDS             complement(321..482)
                     /locus_tag="OO590_003810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638987.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(479..667)
                     /locus_tag="OO590_003811"
     CDS             complement(479..667)
                     /locus_tag="OO590_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6638988.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            927..1262
                     /gene="iraM"
                     /locus_tag="OO590_003812"
     CDS             927..1262
                     /gene="iraM"
                     /locus_tag="OO590_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6638989.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1543..1674)
                     /locus_tag="OO590_003813"
     CDS             complement(1543..1674)
                     /locus_tag="OO590_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR6638990.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2174..2250)
                     /locus_tag="OO590_003814"
     tRNA            complement(2174..2250)
                     /locus_tag="OO590_003814"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2214..2216),aa:Arg,seq:tct)
     gene            complement(2352..2427)
                     /locus_tag="OO590_003815"
     tRNA            complement(2352..2427)
                     /locus_tag="OO590_003815"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2392..2394),aa:Ile,seq:cat)
     gene            complement(2571..3392)
                     /locus_tag="OO590_003816"
     CDS             complement(2571..3392)
                     /locus_tag="OO590_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6638991.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3407..3763)
                     /locus_tag="OO590_003817"
     CDS             complement(3407..3763)
                     /locus_tag="OO590_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6638992.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3776..4825)
                     /locus_tag="OO590_003818"
     CDS             complement(3776..4825)
                     /locus_tag="OO590_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6638993.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRSWVNEKYKRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(4827..5105)
                     /locus_tag="OO590_003819"
     CDS             complement(4827..5105)
                     /locus_tag="OO590_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638994.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            complement(5172..5422)
                     /locus_tag="OO590_003820"
                     /pseudo
     CDS             complement(5172..5422)
                     /locus_tag="OO590_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5640..5795)
                     /gene="hokD"
                     /locus_tag="OO590_003821"
     CDS             complement(5640..5795)
                     /gene="hokD"
                     /locus_tag="OO590_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6638995.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(5867..6154)
                     /gene="relE"
                     /locus_tag="OO590_003822"
     CDS             complement(5867..6154)
                     /gene="relE"
                     /locus_tag="OO590_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR6638996.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            complement(6154..6393)
                     /gene="relB"
                     /locus_tag="OO590_003823"
     CDS             complement(6154..6393)
                     /gene="relB"
                     /locus_tag="OO590_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR6638997.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            6418..>6674
                     /locus_tag="OO590_003824"
     CDS             6418..>6674
                     /locus_tag="OO590_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6638998.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
BASE COUNT         1771 a         1788 c         1401 g         1714 t
ORIGIN      
        1 gtacacctca gtcagatatg tcagcagccc aaacagcaga ctccccagca cgcctattgc
       61 cgcccactga gacggggaaa acctgtccag caactgcagg aaccagtagc ccgttcccac
      121 cgctgacgtg gtgtatgaca cacctgttgt gattttttcc atctggtcca taccccgtct
      181 cccgttatcc ggaagctgac aacaataaaa aagccaccag ttaactactg atggctctga
      241 taactcatgc aagcgtctca gacgacccac tgacactacc ggtgagttta acgatacctt
      301 ccatttgact ggctcacttt ttatgatgat gccggtgcat ttatctccag caccagactt
      361 tctatctcaa cgccatacgt tgcatttttg gtaatatccg tcagcgtcag tgcatttagt
      421 cccactgcca gactgtcttt tatggcctgg aatgccgggc cagtacgatg acgtagtatc
      481 actccggctc agttgcacca ctgaccacca catcaccttc tgctgcaatc gcctgcatca
      541 gggtataagg ggttatggcc accggactac caaacggctg ccagccctct ttcagtttat
      601 gtgtcagctt ttccgcaaga tctgacggcg gcgccgccct gacaacatca tagtgtttaa
      661 atgccatggt tctttccact atctgaaaaa taattcttta aaatacctga catgtaatac
      721 agaaaaaaca caaaaccata ccttaaataa aaacctgatt atcaagcaga tatgcatgga
      781 taaactacaa gacgagatat aaaccaccct gcatttaaat aaacaataaa caacatcaga
      841 aaaataattc tgctctatgg tttaattcaa aaatatcatt tatacttttc agaacatcac
      901 cagcaaggca taaacaagga aactaaatga agtggattgt gattgataca gttatccagc
      961 catcatgcgg aatatctttt tcagtcatat ggagtaaaat aaaattaata atctggtatc
     1021 aatcggatgc tttcttacct cctgaaagta tatttacact gactcacaca ggcatcatgc
     1081 tcaataacaa agtgctacct gtaaccattt acaacgtagt accattcaat aaaacattct
     1141 ggaatttaat caaaaacagc caggaatgcc ctacaaatac agataacgta ttgaatgaat
     1201 gctttaataa ccgttgcact ctgcaaatat gtccttatgg actaaaacaa caaagtccat
     1261 aaggagttta ctcacatctg acaaaatcaa tataaacagc ccctccggag aggggctgga
     1321 gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc cggtgaattc agtaccagca
     1381 cctgaatccg cgattatccc atatacctac tcgctgattg cccctccgca cagggggatt
     1441 caccatgcca gtttctttta acaaactccc cgcaaaccag acaacagtca accgcctgaa
     1501 ttgtgaagta tttaaaaatt tctcccgcta actgataccc ggctaacagt ctggcgtttt
     1561 ctttttcagc aacgggaaag caacaaccac cacacccgcc accagcacac cgtcagccgg
     1621 cactgacatt atccggctgc tgcaatgcca ttcacaaaaa cagtaagcaa tcacttttta
     1681 ccgtaacagg tgataatcca gatatgtatc taccccagat gagtaatccg aagttcatcc
     1741 ataccacagg tcctggctat tctgttgtac tcctgaacaa gagcaaataa ttctgaatta
     1801 gcaaccatga actcatcgca aaccctctgt atagcatcac tattcagaat aataacgtct
     1861 cttcccgaaa gacgatcagg agtacagaac aaaactgtca aacggctgaa ggcctttgct
     1921 cgtgctgcat tgactatatc aatacgctgc ctaaggatga aacaccccga cgcctcatca
     1981 atattcactc tacccacacc atatgaatga taaatactta atgctgaaaa aaccattaga
     2041 ccgtataaca aatactcaat caatacttaa cagaactttt atttttgaca aatatataat
     2101 attttcaaca atatcctgag ccaggtatat ttcagtataa ggctctgccg gaaggaatct
     2161 ggaagaatga atatggcgcg ctgtactgga ttcgaaccag tgaccgattg cttagaaggc
     2221 aattgctctg tccggctgag ctaacaacgc tgaataccga taatggaccg ccatcgggga
     2281 cccgcccccc gcaccaacaa ccctgttatc gtgtcgtctg ctcttcctga taagctaatg
     2341 gcggtttgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     2401 tcgctctcac cactgagcta aagggccggg agcagaataa taacggtccg taattaattt
     2461 cgcaataaaa aacccgctca gcggcgggtt gtagaaactc ttctaacgtc aggcataaaa
     2521 cgcccatcat tatgacgaat ttaccacaga ttccggaaaa atcaaccttg ttacctagtt
     2581 acctttttta actgccgctc agcccatgct tcttcaatat caaaccgggt caccagcgca
     2641 tcataaaatt tcttaactgt tttttcccat gacgcgcgtg ttatctggtt tgtcacctcg
     2701 catatagcat taaatgcctc cgttgatggt agtctttcat agccacgacc accacaacgc
     2761 tggcagtctc tgataacagg cataccacgt tttaccgact cttcacgatg aatggcgaca
     2821 ccacgcccac ggcaatcctt acaggcggtg gaaacctcac cctttccgcc acactccgga
     2881 caggcaactt ttaccacctc cctgactttt ttccattctt cccagtaaga cggatacaca
     2941 cctttcgtac actttgccca taccggcggc ttaccatccg gatactggat cttgtttgta
     3001 aaaactatgc tttcaataaa tttttcccca tagcaacaag ggcactgctt tttactcgct
     3061 gcgctgcggg cataatcctc aaaagcgtac gaagccataa tgcgcatcac taccggtttt
     3121 atttctgccg gaagttttct caacgccgcc acacgatcgc accgactgag tgcataatct
     3181 gccagtaatt ctgttgcccg cgccctgtca ttcatactga tgcccatttt ccccaggaac
     3241 gcagaaaaac ccatctcagc ccgattctgt gtcatgccct gcgcggccat cacatcagtg
     3301 atactcagcg catcttttga cgttgaggcc gatgcatcgg tcaggccagg ggattttggg
     3361 gagtagtatt tcggtaaatc ttccagtttc attttttgac ctgctcttaa tgcattatgg
     3421 ggtaaatctt cacccccaga cgtccaccag atactggctg accacgaacg atattgattt
     3481 catcaaactg ctcatcgtcc attaacactc ccgcatgcgt cagcgcatcc agcggtgctt
     3541 tcaggatatt gtccaggtcg cgacgacgct tatccggtgg ctctgcaatc acctttatcg
     3601 ccagccttcc ggacaggctt aatttcagcc gctgctggcg aacaataagc gccacagccc
     3661 ggcgataacg ctttccctcc tccgagataa aatatgtgct gccacggcgt cgccagtaag
     3721 tgttcaccgt cggcgggtaa ggtaaaacca aatctatgag catcagtcac ctcttttacc
     3781 caagcacgcc agttgcaaag gcgtgatcaa gaaaacgaaa aattaaatca acctgagaac
     3841 catgcttttc ttcgaacgcc agaggatccg catgaagctc gttgtgatgc tcccgacaca
     3901 gcggtagcgt gaaaatatcg tgagattttg tccccattcc gccctgacca tgaccaatca
     3961 ggtgatgggg atcgtcggct ggcttaccac aacacgcaca cggctgtgtc ttcacccagc
     4021 gtttgtattt ctcgttaacc cagctgcgac gtttaggtcg tttcatgaaa gattccggag
     4081 actcaggatc aacggcaatg ctgaccaccg tcttttcctg ttgtgggttc tgttgctggt
     4141 gggcgtgagg cagcggcgca agattttttg tgcgctgttt cagtatgctg gtggcggtct
     4201 gctctcccgg tacgatgtcg ctttcacggt acattgagcg gattttttcc gcacgcaacc
     4261 ccagcgaacg acgtaatacc gcttccggta gcgcgtccgc cacctgattg cggaccgccc
     4321 accaggataa ttcagccaga gataattcac gctcctgcgt accgcttatt gcgtgaccga
     4381 tgacgtcaat catccatgct gacaggtttt gatgagcaag ttgctcgagt gattcggatg
     4441 tctggtcacg cagctggttg tcgcagtgcc agcacaacac cattgcgccg gtaccataac
     4501 ggtgaatgac ggtttcgctg tgatgataat cgccgtgtgg ccactggcag gatttaatat
     4561 ggcgcaacag ccagtcagac aatgcaccag caccaccagc agcacgaatc acccgtgcgt
     4621 tactgaaaaa cggcagcaat gttttgtctt ccaccagcgg ctggcgagcg gcaggaacga
     4681 ccccggacgg cagattacgc atgcttttcg gttccggctc caccagtaac cgggtattgt
     4741 ggaataccgg catggattca cgaccgggct taaggaccac cagcccgagt tccggaacca
     4801 gaacaggtcg aagtaatacc cgcacgttac ctccagatcc gttgctggaa tgtgcgggac
     4861 ggacgcggtg gctgttcgga gtaagggagc ctgacggaga ttatccagtg acggtagtcg
     4921 aggctaaggg cttttttaac ctcgcatccg cgcctgcggt aacactgaat gagccattcg
     4981 gcctgttctt cagtgcatga gggatgctgg taccagtctg acttaaatgc gtgagaatac
     5041 cgctcgtgcg tgtgggcaag aacggtcgaa ttatcatgat tgtaatattt tgcgttgcgt
     5101 gccatcggtt ttctccggtg gcacggtgtt actcagcggg agttcagccc cgcgcaagat
     5161 tgtagatgag tttattcttc tgaaaaagca gaaaagccag cttttattcc gatctctttc
     5221 aatgcctgta atgaagtgac aaactcaccg tcgcgcaaga taaatccgtc cgtcactcgg
     5281 gcatccacaa aattaattaa cgcagcccca ttttttgcaa acacacaatg cggtaatgac
     5341 taacaatatt tccattttca acgcacacag catagaggcc atcttcacaa aaaattttac
     5401 gcagttcttc gatgttcatc atcagaatcc ttccagataa gtagcactcc cctgttcggg
     5461 gtccatccct tttatccctg cgcgctactt aagtattttt gattctattc cggcaccatc
     5521 cagaacttca aatgcgttga aaataaaaac aaaaacccgc cgaagcgggt taagtgcggg
     5581 tgcgttgagg atgcctgcca catcagaggt ggcgagggat ttctcactcg ccgggtctct
     5641 tactcctcag gttcgtaagc tgtgaagaca gcgacctccg tctggccggt tcggattcgt
     5701 acctcgcaga ggtctttcct cgttaccagt gccgtcacta tgacggttaa acagatgacg
     5761 atcagggcga ttaacatcgc cttttgctgc ttcatagcct gcttctcctg tcaacgcaaa
     5821 gcagaagtgt caccttcggt gcgaaacaga gatgtcatgc tttggttcag agaatgcgtt
     5881 tgaccgcctc gctatatacc tccgagcgtt ctcttttccc aacagaaatc acgaaaacga
     5941 caactttctc gtctataacc tggtatacaa ggcgatagcc tgaagaccgg agcttaatct
     6001 tgtaacaatc aggcatacca cggagcttgt ttgcttcaat ccggggtgac tcaagtactt
     6061 caaccagctt ctttttcaac tgttcacgta ccgtcgagcc cagctttcgc cattccttta
     6121 gtgcccgctc gtcaaaatcc agaaaatacg ccatcagagt tcatccagcg tcacacgtac
     6181 tggcttagga ttacgaagcc gttctttcac tatctccaca agttcagcat cttcatcact
     6241 caggagtgtc tgtttgaacg gcaagcgttc attgtcagcg atatactcga gcatgagacg
     6301 aagcgcttca gaaggagtta cacccatttt ttcaagcgcg gcgtaagaac gcgctttaag
     6361 ttcatcgtca atacgcaggt taatgctacc catgtcttac acctcttgta attacaaatg
     6421 tcattacaag tatcgcacta caacatgctt agggcaagtc acgaaggaag tcagaaagta
     6481 gtcgtaagaa cggtgatcac tgtccgcttt gtgccaggag cagccattgc taagtccatc
     6541 ctgtattgtg caggtcagct cttttttaaa gagtccggcc atcatcttac tggtacagac
     6601 accatatact ttgtgacggt caggctacat atgcacaact caacttattc atctattttt
     6661 tgctttagca tgtc
//
LOCUS       DAJTYF010000171         6557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTYF010000171 DAJTYF010000000
VERSION     DAJTYF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.172"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(107..529)
                     /locus_tag="OO590_003825"
                     /pseudo
     CDS             complement(107..529)
                     /locus_tag="OO590_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            847..1488
                     /gene="udk"
                     /locus_tag="OO590_003826"
     CDS             847..1488
                     /gene="udk"
                     /locus_tag="OO590_003826"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6638999.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            1580..2161
                     /gene="dcd"
                     /locus_tag="OO590_003827"
     CDS             1580..2161
                     /gene="dcd"
                     /locus_tag="OO590_003827"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6639000.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            2183..4036
                     /gene="asmA"
                     /locus_tag="OO590_003828"
     CDS             2183..4036
                     /gene="asmA"
                     /locus_tag="OO590_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6639001.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4488..6071)
                     /locus_tag="OO590_003829"
     CDS             complement(4488..6071)
                     /locus_tag="OO590_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6639002.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(6272..6421)
                     /locus_tag="OO590_003830"
     CDS             complement(6272..6421)
                     /locus_tag="OO590_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639003.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
BASE COUNT         1653 a         1664 c         1717 g         1523 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacgggac
       61 tttttatacc ccgcaggggt cataacatgc ttgactactt aataggggca ccccccaaca
      121 gaggcggaac aatggcgctg ccgaatgcca gacgcagcca gtcagccaga ttttgcaagg
      181 ggttatacca cggcagcaat ttacgtagca gcactgcccc gaccacggct tcaacaatat
      241 tgatggtcgt ccaggtcatg ttcagcgagc tggtggaaaa aagcaggatg gatgcggcga
      301 tatttcccag cgaacagctc agcgcaattc ccggccacat gcgcccggca tggcgataaa
      361 acgccaccat catgatggac gtcgggaacc acagtggggc gagttgggtg ccaaactgcg
      421 aaagctcaag cgagacaaga gtaaagataa acgagactaa acctaaactg accaggtgaa
      481 gcagcgggtg gggcagggca attaatacat gctgtgattg tttgcttatt accgctttat
      541 ccgcaaggcc aggtcgccag tattcgtttc tgtgtttatg ctagtacaaa agttttacat
      601 tttatatgcg aattgctcat aaagtgacgt aaaggcggat aatttgcgca actgcgtata
      661 acatttttta ccttacataa aactgatcaa cgtaatttgc caccgggaat tctttgtcaa
      721 caattaactc tgagcgcgcg gcaacgctat tcgactggta tcagacggat gaaatcccta
      781 taattgccgc gtttggcgct tcgtcgcccc cttcctaaca tccaggttaa tcaggtcgct
      841 aaatatatga ctgatcagtc tcatcagtgc gtcattatcg gtatcgctgg cgcatcggct
      901 tccggcaaga gtcttattgc cagtaccctt tatcgtgaat tacgtgagca agtcggtgat
      961 gaacacatcg gcgtaattcc cgaagactgc tattacaaag atcaaagcca tctgtcgatg
     1021 gaagaacgcg ttaagaccaa ctacgaccat cccagcgcga tggatcacag cctgttgctt
     1081 gagcatttac aagcgttgaa acgcggctcg gcaattgacc tgccggttta cagctatgtt
     1141 gaacatacgc gtatgaaaga aacggtgaag gttgagccga agaaggtcat cattctcgaa
     1201 ggcattttgt tgctgacgga tgcgcgtttg cgtgacgaac ttaacttctc cattttcgtt
     1261 gataccccgc tggatatctg cctgatgcgc cgcatcaagc gtgacgttaa cgagcgtggg
     1321 cgttcaatgg attccgtgat ggcgcaatat caaaaaaccg tgcgcccgat gttcctgcaa
     1381 ttcattgagc cttctaaaca atatgcggac attatcgtgc cgcgcggcgg gaaaaaccgc
     1441 atcgcgatcg atatattgaa agcgaaaata agtcagttct ttgaataagc ttgataaatt
     1501 gtgtaccgtt cagtgataag ctggtatgcc cttgacgtaa ttggcgttaa gggagtgatg
     1561 cgcgaaagga gaaaatgcca tgcgtctgtg tgaccgagat attgaagcct ggcttgatga
     1621 aggccgtttg tcgatcaacc cacgtccgcc agtggagcgt attaacggcg cgacggtgga
     1681 tgtacgcctg ggcaataaat ttcgtacctt ccgtggtcac acggcagcgt ttatcgatct
     1741 gagcggtccc aaagatgaag tgagcgccgc gcttgaccgc gtgatgagcg atgagatcgt
     1801 tctcgacgag ggcgaggcgt tctatcttca cccaggagag ctggcgctgg cggtgacgct
     1861 ggagtcggtg acgctgccag ccgatctggt gggctggctg gacgggcgtt cctctttggc
     1921 gcgtctgggg ctgatggtgc acgtcaccgc gcaccgtatc gatccgggct ggtctggttg
     1981 cattgtgctg gagttctaca actccggtaa gctgccgctg gcgctgcgtc cgggcatgtt
     2041 aattggtgcg ctgagctttg agccgctttc cggcccggcg gcgcgacctt acaaccgccg
     2101 tgaagatgcg aaatatcgca accagcaggg cgcggtagcc agccgaatcg ataaagacta
     2161 attcaggccc attgaggata ccatgagacg atttctgacg acgctgatga tactcctggt
     2221 cgtgctggtg gccgggttat ctgcgttagt gttgctggtg aatccgaatg atttccgcga
     2281 ctatatggtc aagcaagttg ctgcacgtag cggttatcaa ttgcagctcg acgggccact
     2341 gcgttggcac gtctggccgc agcttagtat cctctccggg cgaatgtctc tcaccgccca
     2401 gggcgcaagc cagccactgg ttcgcgccga caacatgcgt ctggacgtgg cgcttttacc
     2461 actactgagt catcaactga gcgttaagca ggtgatgcta aaaggggcag tgatccaact
     2521 gacgccgcag acggaagcgg tgcgcagtga agacgctccg gttgcaccgc gcgacaatac
     2581 cttgccggat ctgtcagacg atcgcggatg gtcgtttgat atatccagtc ttaaggtggc
     2641 ggacagcgtt ctggtgttcc agcatgaaga tgacgagcag gtgacaatcc gcaatatccg
     2701 cctgcaaatg gaacaagatc cccaacatcg tggctcattt gagttctccg ggcgggttaa
     2761 tcgcgatcag cgcgatctca cgatatccct taacggtacg gtagatgctt ctgattatcc
     2821 gcatgattta acagcggcta ttgaacaaat taactggcag ttgcagggtg ccgatttacc
     2881 aaaacaaggt attcagggac aggggagttt ccagtcccag tggcaggagt cacataaacg
     2941 cctttcattt aaccaaatta gtttgaccgc caatgacagt acgctgagcg ggcaagcaca
     3001 ggtcacgctg acagagaaac cggaatggca gctgagactg caattcccgc aactgaatct
     3061 tgacaacctc atcccgctta atgaaaccgc gaatggtgaa aacggtgccg cgcagcaggg
     3121 gcagagccaa tcaacgttgc cgcgcccggt catttcttcg cgtattgatg aaccggccta
     3181 tcagggactg caaggcttta cagctgatat tttgttgcag gccagtaacg tgcgctggcg
     3241 cggaatgaat tttacagatg ttgccacgca aatgaccaac aagtcgggtt tgctggaaat
     3301 tactcaactg cagggcaagc ttaacggtgg aaaggtttca ctgccgggca cgctggacgc
     3361 gacatcaata aatccgcgga taaacttcca gccacggctg gagaacgttg agattggtac
     3421 cattctgaag gcgtttaact atccgatttc gttgaccgga aaaatgtcac tggctggtga
     3481 tttctccggt gctgacatag atgccgacgc attccgccac aactggcaag ggcaggcgca
     3541 tgttgagatg accgacacac gtatggaagg gatgaacttc cagcagatga ttcagcaagc
     3601 ggtagagcgt aatggcggtg atgtgaaggc cgctgaaaac ttcgataacg tgacgcggct
     3661 tgaccgcttt accaccgatt tgacgttgaa ggatggcgtc gtgacgttaa acgacatgca
     3721 aggtcaatcg cctgtgctgg cgctgacagg ggaaggcatg ttgaatctgg cagatcaaac
     3781 ctgcgacacc cagtttgata ttcgggtcgt gggtggctgg aacggggaaa gcaaactgat
     3841 tgatttcctc aaagaaacgc cagtaccgct gcgggtttat ggcaactggc agcaactcaa
     3901 ttacagcctg caagtggatc agttactgcg caaacatcta caggacgaag cgaaacgtcg
     3961 cctgaatgac tgggccgagc ggaataaaga ttcccgcaat ggcaaagatg tgaagaagtt
     4021 gctggagaag atgtaggtta ccaggtgacc tgtcctgtag gcctgataag acgcgtaaag
     4081 cgtcgcatca ggcgttcgcg gcggcactat tatattctga atatgtaaga tcgaaggctg
     4141 ttccgttcac ttgatgttcc tcgctactat ggaacgctca agccggtgaa cgtgccaggg
     4201 tggctcaatc gccaccaccc tggcaacccg ggctcccggc ggtaaatcgc cgctggcgcg
     4261 gttccctcat cttattcctt tcggctatcg ggtacgggcg cgaggtaaca tccctgtaaa
     4321 acgcgccctc agcccacatc catgtgggct gccccagcct acaggaaacg attcggcgat
     4381 ttacagccgg acccacaccc ccgctgtgat gtccgtttac cacagacaac gcttcattgc
     4441 tgaagttata aggcgttacg cagcattggg taaaatggcc gataccatca cacctcgtac
     4501 tccatctcgc catccttacg cagcggtata atctgcacct tctgcacgcg atggctttcc
     4561 acctgcaacg ttttaagcaa ataatccccc acctgaactt cttcgccagg ttttggaata
     4621 cgctgcaaat actccatcaa cagcccggca atggtgtgat attcacgttt ttcatccagc
     4681 ggcagcggca catattgcac cagatcctcc agcggcatat gaccattcgc cgtccaggaa
     4741 ccgtccgcat tcttctgaat atcatggcgg gcgtcgatct cttccacttc gttcggtaag
     4801 ttaccggcaa tggtttcagt gacgtcactt aatgtcacaa tcccttccac cgagccaaac
     4861 tcatccacca caaaagcaaa gtgcgtgcgg gcattacgga actgctccag ggctggtaac
     4921 aacggcaagg tttccgggaa caccagcggc tggcgaatca acacccgcag gttgagcggt
     4981 tcgccgcgca gtgactgttg tagcaggtcg ataacgtgaa caacaccgag caaatcttct
     5041 gcgtcatcac cgtcggtaac caccagccgc gtatgctgat ttcgctccag caactggcgg
     5101 atttcttctt ccggcgcgtt gagatcgata tgctcaatat cgtggcgcga cgtcataatg
     5161 ctgctgacgg tacgctggtt aagattaagt acccgctcaa tcatccgccg ttcctgcgga
     5221 ttaaagatct gctggtttcc atgatccacc agcatggacg cggtttcggc gtccagttcc
     5281 gcatcttctt tttgcccgct aagcaggcgc attaccgctt cggtagtccg ctggcgtagc
     5341 gtctgattag cggaaagaaa acgtcgccgg ttaaagatag ccaactgatt gagtgcctcg
     5401 atcatcaccg agaaaccaat ggcagcgtac aggtagcctt tcggaatgac gaagccgaaa
     5461 ccttctgcca ccaggctaaa gccaatcatc aacaggaagc ttaagcagag aataacgatg
     5521 gtcgggtgac tgttaacaaa ttgcgttaac ggcttgctgg ccatcaacat caggctgata
     5581 gcgataacca cggcggccat catgaccagt aaatggtcga ccatcccgac ggcggtaatc
     5641 actgagtcga gtgagaaaat ggcgtccagt accacaattt gcgtcaccac gccccagaac
     5701 ttcgcacctt tgcgttgtgt gggattattg ctgtctttcc cttccagccg ttcgttcagc
     5761 tccatcgtgg ctttgaacaa caggaagaaa ccaccaaaca gcatgattaa gtcgcgggcg
     5821 ctaaaggtaa acgagcggaa gctgaacagc ggttgagtca gggtgaccag ccaggagatt
     5881 gacgctagca gtaacaggcg cattaacatc gccagcagca gcccggtaac ccgtgcgcgg
     5941 tcgcgctgct tcggcggtag tttttcggcg aggatggcaa taaagaccag gttatcaatg
     6001 ccgaggacca gttcgatcac aatcagcgtg attaacccgg cccagataga cggatcggca
     6061 atccattcca tagtaagtac aaaacctatt cgttgtatga caaatgtcac aattcgatca
     6121 tggataaatc ggggcaaaaa tgcaatgttg cagcactatt tatctcatat tatttgaggg
     6181 taataacctc acattatccc tgaattaaaa gcggtaataa taaaacatta tttaagaaat
     6241 atcgcatgtg gagacgcaat attaaatatt gtcaacctaa agaaactcct aaaaaccata
     6301 ttgaatgaca cttaatataa ttcttaaaaa tagccaattg ccgaattgtt accttgcctg
     6361 ctattccgtt agctgtaaca cttcctcctg cattattggc gagccaatat tcaattacca
     6421 taatgtcacc ttatattcgg aagttaattt ccgcaataac cctttctcat caacgattgt
     6481 atgcagagag ggtttgaaaa attaagctca atcagtgtat tggtagctaa aaagccaggg
     6541 gcggtagcgt gtctgga
//
LOCUS       DAJTYF010000172         6545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTYF010000172 DAJTYF010000000
VERSION     DAJTYF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.173"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..2422
                     /locus_tag="OO590_003831"
     CDS             <1..2422
                     /locus_tag="OO590_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6639004.1"
                     /translation="NLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGKVIVR
                     GKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPFVFAT
                     NGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQDYYDD
                     GSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKNKKRI
                     LFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQKAFK
                     QVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSSTDYFG
                     DPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYNQKDF
                     DRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQVKKN
                     DKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQNIQS
                     MTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPEDIADP
                     ESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHVNGVA
                     VGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALIHELE
                     QQGIIWEVLAEEVGKDLDPFDILCHVVYGQPPLTRKERAENVHKRNYFTKYSEAAQAV
                     LDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSELENEI
                     YQLPPRSA"
     gene            2489..3949
                     /locus_tag="OO590_003832"
     CDS             2489..3949
                     /locus_tag="OO590_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6639005.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGK"
     gene            3958..5619
                     /locus_tag="OO590_003833"
     CDS             3958..5619
                     /locus_tag="OO590_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6639006.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5840..6181
                     /gene="symE"
                     /locus_tag="OO590_003834"
     CDS             5840..6181
                     /gene="symE"
                     /locus_tag="OO590_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR6639007.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6228..>6545)
                     /locus_tag="OO590_003835"
     CDS             complement(6228..>6545)
                     /locus_tag="OO590_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639008.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         1928 a         1565 c         1507 g         1545 t
ORIGIN      
        1 gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc cagccattct
       61 caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc gggacggtaa
      121 agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg acatcgtgct
      181 gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca aacatgaaat
      241 tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc cttttgtttt
      301 tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag gtgaatgcct
      361 tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc aaaaattctg
      421 cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact attacgacga
      481 tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa ccattgaagc
      541 cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag ggaaaaccta
      601 taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac gcattttgtt
      661 ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc agccatttgg
      721 tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa ttcacctcgc
      781 gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac aagtcgcacc
      841 agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat ccgaagacag
      901 cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct taaccgccac
      961 gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg tttacgtcta
     1021 ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg tccgtgttga
     1081 tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact taaacggcga
     1141 agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg tgatcgacga
     1201 acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca atccgatgga
     1261 taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc gcgccctggt
     1321 taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa tcaccggcga
     1381 tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac cgtacccggt
     1441 tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct gcaaactggt
     1501 agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg gtcgtggtac
     1561 acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta aaaaagccac
     1621 cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg ataccacacc
     1681 agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa tcagcgaaca
     1741 tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc aggttaccga
     1801 taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca agtttcacgt
     1861 caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg ccgacggtaa
     1921 actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag aatatgcctc
     1981 gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcgc tcattcacga
     2041 gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta aagatctcga
     2101 cccgttcgac attctttgcc acgtagtgta tggtcagccg ccgttaaccc gcaaagagcg
     2161 cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag cgcaagccgt
     2221 gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg aaagtattca
     2281 ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta ttaaaaccgg
     2341 atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg aaatctacca
     2401 attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca tcatgttgca
     2461 gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca aatcattaca
     2521 agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg gtcagctctc
     2581 ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac tggagcagga
     2641 taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg ccaacgcgca
     2701 gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc cggcgttgaa
     2761 aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc aggcgttcag
     2821 cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca acaagctgaa
     2881 cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt acgaacagat
     2941 ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac gcgccgtcac
     3001 tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg acccggcctg
     3061 cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat acgtgaagag
     3121 cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa aacagcttcc
     3181 gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg tacaaattcg
     3241 tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg atgtcattgt
     3301 taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact ttccggcaga
     3361 gatgcaaacc cgcgaaacgg cggatctgtt cctgcaactg attgtggaag tactggcgaa
     3421 aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag gtgttaaaac
     3481 caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc gtttaccgaa
     3541 tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta ccaaaggtca
     3601 gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga aaaactacag
     3661 caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt ggggtaacga
     3721 ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca gcattgatga
     3781 cgtgattgca cgcaacttca atctggatat taaaaaccca catcaggcgg aaaccgtcag
     3841 ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga tccagacgct
     3901 gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaagtagg ttaactaatg
     3961 agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt gcaaacccgt
     4021 tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa gaaattgcgt
     4081 gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc gaatgatgaa
     4141 ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt gaagcaaggc
     4201 aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc gtttgaattg
     4261 ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc tgagtcatct
     4321 agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat cgagcaaagc
     4381 caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt aaataatcca
     4441 gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga ttttgttgtc
     4501 ggcgctgacg gtgtgaaaat tttatcacct atattaattt gtgagcgttt ctttttttgg
     4561 cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa agtattaaat
     4621 agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga aaaagttagc
     4681 tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct ggacgcacat
     4741 cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac cgcggaactg
     4801 gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac cgaagccagc
     4861 gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact tgtgccgcag
     4921 gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga aaaagctcaa
     4981 ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag cgatgaggaa
     5041 aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat agttaatatt
     5101 aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc acctgatatt
     5161 attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc aggagtcaaa
     5221 gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg cccgtcatta
     5281 tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta tccattagac
     5341 tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt tcttatgcaa
     5401 gtcaaaaaag cagagaacag aataaaaatg ccgaaactta attctgacag tttttacaat
     5461 attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa gataaattct
     5521 attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa aacccaactg
     5581 catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc tcacaaaacc
     5641 aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt cacgctaatt
     5701 ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga tgatatatcc
     5761 aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc acaatcacta
     5821 tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt tagaagcaga
     5881 agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt acccggatta
     5941 cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg gttttgccac
     6001 cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg cccaacccgc
     6061 cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc tgtcggcgcg
     6121 taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga aagtcgcctg
     6181 atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta taccaacgcg
     6241 ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg aagttgtgcg
     6301 aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt tgtcacatct
     6361 ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga caatcgcagt
     6421 cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc aatatatagc
     6481 ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg aagatgatcg
     6541 aataa
//
LOCUS       DAJTYF010000173         6360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTYF010000173 DAJTYF010000000
VERSION     DAJTYF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.174"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            163..270
                     /locus_tag="OO590_003836"
                     /pseudo
     CDS             163..270
                     /locus_tag="OO590_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            267..557
                     /gene="tnpB"
                     /locus_tag="OO590_003837"
     CDS             267..557
                     /gene="tnpB"
                     /locus_tag="OO590_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6639009.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAML"
     gene            complement(809..1552)
                     /locus_tag="OO590_003838"
     CDS             complement(809..1552)
                     /locus_tag="OO590_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR6639010.1"
                     /translation="MVKILAVAQSHESIVSMDEINSILLSSSWFRALPDYLIEQMLSR
                     TSLYQYNDGEIIHYRGAEPLGLYAVITGSVKVSSISADGKECIFRYLSPGNWFGEIGM
                     IDKCPRTHDARATGKTTLLTIKPHDVEFLLKNYPILHRFLALLLCKVVRNAFTMLHDC
                     TLLSVSARLAKRLVSLSEGYGEPHERGTLINLYLTQDDLATIINTSRQTINKRLVEWE
                     KLGWIDARYGKIVIININALRQLYADEDE"
     gene            complement(1602..2969)
                     /locus_tag="OO590_003839"
     CDS             complement(1602..2969)
                     /locus_tag="OO590_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6639011.1"
                     /translation="MPSHTDQYLSLAIQKSIRRIIPMVILMFILAYLDRSNIGFAKQE
                     FQLDTSLSDTAYAFGAGIFFIGYALFEVPSNILLYRIGARVWLARIMVTWGLVSAAMM
                     FAHTETVFIFLRFLLGISEAGFFPGVILYLTFWFPQHIRARVTGYFLFGAPLAFIIGG
                     PLSGSLLSLEGSVLSFGLHGWQLMFLVEGLLASAVGIWVFFYLDDHPEKAKWLTPQEK
                     AALSQTLNKEEQVKLAHGPKGAWQALLDVRVLYLCLIWFTVQVCGYGIYFFLPTQIAT
                     LIGTKVGLLVGFVTAIPPLCAAIAVYYVPRISEKLQERRYVASITFMSGAIGIAVSGL
                     ADSIPLVAIVALCVAAAGHLAMQPLYWTFPSSYLGGAAAASGIALINSIGNLGGFVAP
                     NLRVWAEHTFNSAQAGLYLLAMVTATGSLMILTLRHFGIEKTPRTSSTSPNLPASGDN
                     LHHSD"
     gene            complement(3041..4153)
                     /locus_tag="OO590_003840"
     CDS             complement(3041..4153)
                     /locus_tag="OO590_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HCR6639012.1"
                     /translation="MKITKLTTFIMHVPVTKNIIGDSTHSITHWGMPGVMIETDTGLV
                     GYGYTGTHADITTDRLITTIIKDAFGPMLIGEDASEVRYLHRKMSRSSTNIWVGRGGL
                     MQMALSAIDIALWDLKAKMVEQPLWKVFGGSQHSRVLAYNTDCGWLVRNTAELVDDCK
                     KMIFEEGFSAIKMKIGKPDPREDLKRIEAVRKAIGDDVNLMVDANAKWDISTAKQYGA
                     KLADFDITWFEEPMWHDDIASHRTLSGHISTPIALGELLYQFDSFKEFVLAGAVDYLQ
                     PDATRCGGLTAVWEIADLGQAFNLPVTPHHGDMMQAQLHLVMAHPSCSLLEFIPWTLG
                     CFVDPVEVKEGYYSLPTAPGAGTTLRPEALEHFSVR"
     gene            4319..4800
                     /locus_tag="OO590_003841"
                     /pseudo
     CDS             4319..4800
                     /locus_tag="OO590_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
     gene            4861..5294
                     /locus_tag="OO590_003842"
                     /pseudo
     CDS             4861..5294
                     /locus_tag="OO590_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5350..6360
                     /locus_tag="OO590_003843"
                     /pseudo
     CDS             5350..6360
                     /locus_tag="OO590_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    5630..5746
                     /locus_tag="OO590_003843"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1734 a         1530 c         1604 g         1492 t
ORIGIN      
        1 ctctggcaga acgaagggcg tatatcacgg cgtcttcagg taacgacctc ttctgacact
       61 ggcgttgaat tattacctgt agaaataacg ccggatgagc agaaaaaaac tgtggcggcc
      121 attgcgccgt ctttatccac ttccactcag accagagtca gtgccagttc ctgcaaggtg
      181 gagttccgtc acggtaacat gacgctggaa aatccatcgc cagagctgct cacggtgttg
      241 atccgcgaac tgagcgggag gggaagatga tttcactccc atcaggtacc cgtatctggc
      301 tcgttgccgg cgttaccgat atgcgtaaat ccttcaacgg actgggagaa caggtacaac
      361 atgtgctgaa tgataatccc ttctccggcc acctgtttat cttccgtggc cgacggggtg
      421 acaccgttaa aattctttgg gctgatgctg atggtctgtg cctgttcacc aaacgccttg
      481 aggaaggtca gtttatctgg cctgcggtgc gtgacggtaa gatatccatt acccgctcgc
      541 aactggcaat gctgtaaacg ttaacggagc gccgtattga cgcttacgaa ctgtgtgatc
      601 cgacatgcca ttaggaaacg taaggcgttc ccgatgggcg agtcagtaaa agtgaaatgg
      661 ctggcatcct ggtcgcgtcg cagaaatgga caatgccgca gagggactgg cgaatagcaa
      721 tttattatcg aatttgatga ccgcctggac agtcactttt aagaaaaagc atgtatacag
      781 aatggtaaac aggctcatcc acagccaatt actcatcttc atcagcatat aactgcctga
      841 gtgcatttat attgatgatc acgattttgc catatctggc gtctatccat cccaattttt
      901 cccattcaac cagccgtttg ttgatcgttt gtcggctcgt attgattatc gttgccagat
      961 cgtcctgcgt aagataaaga ttgattaatg tccctcgctc gtgtggttcg ccgtaccctt
     1021 ccgacagact cactaaacgc ttcgccaagc gtgcagaaac agataatagc gtacagtcat
     1081 gtaacatcgt aaaggcattt cgaacgactt tgcaaagcag caacgccaga aaacgatgga
     1141 gaattggata atttttcaga agaaattcaa catcatgagg cttgatcgtt aacagtgtag
     1201 ttttacccgt cgctctggca tcgtgtgtgc gcggacattt atcaatcata cctatttcac
     1261 caaaccagtt ccccggcgaa agatagcgga agatgcactc tttaccgtca gcagaaattg
     1321 agctcacttt aacggagcca gtaatgactg catataatcc gagcggctca gcaccacgat
     1381 agtgaataat ctctccatcg ttgtattgat acaggctggt cctggacaac atctgttcaa
     1441 taagataatc aggtagcgcc cgaaaccaac tactactaag aaggatacta ttgatctcgt
     1501 ccatagaaac tatcgattca tgtgattgtg ctacggccaa tatcttaacc attgttgtta
     1561 tcctgatctt aatcatgacc gtagcaatgt agcatttcct gtcaatcaga gtgatgcaga
     1621 ttatccccgc tggcagggag attcggagat gtggatgatg tacggggcgt tttttcaatc
     1681 ccgaagtgac gtaatgtcag aatcatcagg ctaccagtag ctgttaccat tgccagtaag
     1741 taaagtcctg cctgtgcgga attaaaagta tgttccgccc agacgcgaag attcggcgct
     1801 acaaaccccc ccagattgcc tatcgagtta attaacgcaa tgccggaagc agctgctgca
     1861 ccgccgagat aagacgatgg aaatgtccag tacagtggct gcatcgccag atgtcctgca
     1921 gcggcaacgc acagcgcgac aatagcgacc agtggaatac tatcggcaag gccagatacg
     1981 gcaatgccta ttgcaccgga cataaatgtg attgatgcaa cgtaacgacg ttcctgcagt
     2041 ttttccgata tacgtggtac gtaatacact gcgatggcag cgcacaaagg tggaatagcg
     2101 gtgacgaagc caactaataa accaactttt gtccctatta aggtggcgat ttgtgttggc
     2161 agaaagaagt aaattccata accacatacc tgcaccgtaa accagatcag gcataaatag
     2221 aggacgcgta catccagcag tgcttgccat gcgccttttg gaccatgtgc aagtttcacc
     2281 tgctcttcct tatttagagt ctggctaagc gccgctttct cttgtggagt taaccatttg
     2341 gctttctctg ggtgatcgtc aagatagaag aacacccaga tcccaacggc agaggccagt
     2401 aacccctcaa cgaggaacat gagttgccag ccatggagac caaacgacag taccgatcct
     2461 tcaagcgaga gtagcgagcc ggaaagcgga ccgccgatga taaaagctag tggggcacca
     2521 aacaggaaat agccggtgac gcgtgcgcgg atgtgctggg ggaaccagaa tgtaagataa
     2581 agaataactc caggaaagaa acctgcttca ctgatgccaa gcaagaagcg caggaaaata
     2641 aatacggtct cagtgtgggc gaacatcatt gcagcggaaa ccagccccca ggtcaccatg
     2701 atacgggcca gccagacacg ggcgccgatg cgataaagca gaatattact gggcacctcg
     2761 aacagggcat aaccaataaa gaagatcccg gcgccgaaag cataggcggt gtcactcaga
     2821 ctggtatcca gttgaaattc ttgtttagca aagccgatat tggagcgatc cagataggcc
     2881 agaataaaca ttagtatgac catcggaatg atgcgacgta tggatttttg aattgcaagt
     2941 gataaatact gatcggtatg gctgggcata aatcacctct tagaattttg tcattattat
     3001 tttgccccgg tcattgcata accgggcgat acaagtgttc tcatctaacg ctaaagtgct
     3061 caagagcttc cgggcgtaag gtagtgccgg cacccggcgc ggtgggaaga gagtaatatc
     3121 cctctttgac ctcaaccgga tcgacaaagc agcccagcgt ccagggaata aactccaaca
     3181 gagagcaaga gggatgtgcc attaccagat gtaattgtgc ctgcatcata tcaccatgat
     3241 gtggtgtgac cggcagattg aacgcctgac cgagatcggc tatttcccat acggcggtca
     3301 gtccaccgca gcgagtggca tcgggctgga gataatccac cgcaccggcc agtacaaact
     3361 ctttaaagga atcaaactga tagagtaact cacccaacgc aatcggcgta ctgatatgcc
     3421 cggacagcgt gcggtgactt gcgatatcat catgccacat cggctcttca aaccaggtaa
     3481 tatcgaaatc agccagcttg gcaccgtact gcttggcagt gctgatgtcc cattttgcat
     3541 tcgcgtcgac catcagattg acgtcatcgc ctatcgcttt tctgacggct tcgattcgtt
     3601 tgagatcttc tcgcggatcg ggcttgccta tcttcatctt gatggcggaa aatccctcct
     3661 caaaaatcat cttcttgcag tcatccacta actccgccgt gttacgaacc agccagccgc
     3721 agtcggtgtt gtaggcaaga acacgggaat gttgagaacc gccaaagact ttccacaaag
     3781 gttgctccac cattttggct ttcaggtccc acagggcgat atcgattgct gaaagcgcca
     3841 tttgcattaa cccaccgcgg cccacccaga tattggtcga gctgcgtgac atcttacggt
     3901 ggagatagcg aacttcactg gcgtcttctc caatcaacat cgggccgaag gcatctttaa
     3961 taatggtggt gataagacgg tcggtagtaa tgtctgcatg ggttccggta taaccgtaac
     4021 ctacaaggcc ggtatctgtc tcgatcatta cgcccggcat tccccaatga gtaatgctgt
     4081 gtgtggagtc accgataata tttttagtta ccggtacatg cataataaag gtagtgagtt
     4141 tagtgatttt cattgcagtt tcctgtgtct gtatatcttg attgatgggc gcaatgtagc
     4201 aaccggcctt cagcagggct gtcaaatggt tgacacatca tcagaagaga gaaggaataa
     4261 ttgtattttt ttgaagtaga tctcgaattt ggaccacacc cagtcgacaa gttttcatat
     4321 agggaactgg gccatgaccg gagcttttca ggatgagatc tctgtacgca tcatgaagac
     4381 catgcggcta aagtgatcct ggagtacagc aaactggaaa aaatcgaaaa gtattacgaa
     4441 aaatacgcaa acgcaggcac caatgagtgg cacatcgatg aaagtaaaaa agctaccgca
     4501 accatgaatg tagttctggg tgatgaagat aggttaaaat ccctcgcgga agattttgcc
     4561 agacattatg aaaaacgcgt agccgaaggt ttcaccgtaa aaggcaagac catgtttgtg
     4621 ccagccgtga aattgcctag ggttctaccg tcaccttgaa gctattcgca ctgcctggtt
     4681 ttaagtgaaa caagtacccg atggcatcgt cctgactgaa cgggagcaga aaaattaccg
     4741 ccttctgaaa tggtgaaaat ggtcatgacg cgtggtaaag atgatgacga ggcgctttat
     4801 agatttgtgt aattgcctga ttttgatatg ttcaattcaa catcaaatga aggttaattt
     4861 atggacgaaa aatagttaca gactctggct aacgaactgg ccaaaaacct caaaacccct
     4921 gaagacctca gccagtttga ccgtctactg aaaaagatca gcgttgaagc cgctctcaac
     4981 gcagagatga cacaccatcc tgggtatgga aaaatcagtc cagaccagga gctaactccc
     5041 gcaacggtta ttccacaaag accgttatca caggcgacgg tccactggaa ctgcgtactc
     5101 cgcgcgatcg tgacggtagc ttcgaaccac aacaggtgaa gaaaaatcag ccccgtatta
     5161 ccaggatgga taaccagatc ctctcgttgt atgccaaagg gataaccacc cgtgagatag
     5221 ccgccgcgtt caaagacctg tatgacgctg atgtttcacc ggcactgata tcaaaggtca
     5281 ctgatgccgt gatggtgaac cgccccggaa atcctggaga ctaaactccc tgagaaagag
     5341 gtaaacagga tgactaaaaa tacacgtttt cccccgaaat ccgtcaacgg gcagttcgta
     5401 tggttctgga aagtcagggc gaatatgact cacaatgggc agcaatttgc tccattgctc
     5461 caaagactgg ctgtacgccg gagactctgc gtgtctgggt tcgccagtat gagcgggata
     5521 ccgggggcgg agatggaggg ctcaccagtg ctgaacgtca gcgtctgaaa gagctggaac
     5581 gtgaaaatcg tgaactgcgc cgcagcaaca atatccttcg ccaggcttcc gcttattttg
     5641 tgaaggagga gttcgaccgc ctctggaaaa aatgatgcca ctgctggata agctgcgtga
     5701 gcagtacgga gtcagaccgg tatgcagcga actgcatatt gccccgtcaa cgtattacca
     5761 ttgtcagcaa cagcgacatc atccggataa acgcagtgcc cgtgcgcagc acgatgactg
     5821 gctgaagaaa gagatacagc gcgtatacga tgaaaatcat caggtgtacg gtgtgcgtaa
     5881 agtctggcgt cagttgttac gggaaggaat cagggtggcc agatgtacag tggcgcgcct
     5941 catggcggtt atgggacttg ccggtgttct ccggggtaaa aaggtccgca ctaccgtcag
     6001 ccggaaaacc gttgccacag gtgaccgcgt aaaccgtcag ttcgtggcag aacgtcctga
     6061 ccagctgtgg gtggctgatt ttacttacgt cagcacatgg cagggcttcg tctatgtggc
     6121 gtttatcatt gatgtgtttg ccggatacat cgtggggtgg cgggtctcat cgtctatgga
     6181 aacgacattc gtgctggatg cgctggagca ggcgttgtgg gcccgtcgtc cgtctggcac
     6241 catccatcac agcgataaag gctctcagta tgtgtcactg gcctatacgg agcgactaaa
     6301 agaagccgga ttactggcat caacagggag tacaggcgac tcgtatgaca acgcgatggc
//
LOCUS       DAJTYF010000174         6337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTYF010000174 DAJTYF010000000
VERSION     DAJTYF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.175"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OO590_003844"
     CDS             complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OO590_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR6639013.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            322..1953
                     /gene="dcuS"
                     /locus_tag="OO590_003845"
     CDS             322..1953
                     /gene="dcuS"
                     /locus_tag="OO590_003845"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6639014.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1950..2669
                     /gene="dcuR"
                     /locus_tag="OO590_003846"
     CDS             1950..2669
                     /gene="dcuR"
                     /locus_tag="OO590_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6639015.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3240..4580
                     /gene="dcuB"
                     /locus_tag="OO590_003847"
     CDS             3240..4580
                     /gene="dcuB"
                     /locus_tag="OO590_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6639016.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4658..6304
                     /gene="fumB"
                     /locus_tag="OO590_003848"
     CDS             4658..6304
                     /gene="fumB"
                     /locus_tag="OO590_003848"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6639017.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAVIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
BASE COUNT         1633 a         1620 c         1558 g         1526 t
ORIGIN      
        1 cgactttcgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
       61 acatatcgca gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc
      121 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc
      181 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat
      241 atagccaatc atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
      301 atttgtcaca caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt
      361 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
      421 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc
      481 aacaaggcac tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
      541 cagaaaaaac cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac
      601 gatctgctgt ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc
      661 cagcgtattg gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa
      721 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
      781 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
      841 caacagatca atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc
      901 ggactgattg gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa
      961 ccctacgaaa tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa
     1021 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     1081 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg
     1141 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag
     1201 attacgatta aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt
     1261 atcatcggtg ccatttcaac cttcagggac aaaactgaag ttcgtaaact gatgcagcga
     1321 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     1381 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     1441 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     1501 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     1561 acgctgattt taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg
     1621 acgctgatta ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa
     1681 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     1741 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     1801 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     1861 ctcggcggca gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag
     1921 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg
     1981 caatggtcgc ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg
     2041 gaacagcctc gacgctggag aaagccaaag agattatctt caatagcgat gcgcctatcg
     2101 acctgatatt gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     2161 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa
     2221 ccattaaaga ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt
     2281 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     2341 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg
     2401 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     2461 ttgacgcgca tcaggactat gacttttcaa ccgacgagct ggctaacgag gttaacatct
     2521 cgcgtgtttc ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca
     2581 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     2641 actcactgct gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     2701 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc
     2761 agatcaaata taattatccc tccatcatcg ttaaaaatta atatctcttc agatgaacgg
     2821 tgtttttaat ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt
     2881 gtatttttac ccacaaatgg gtagatcaga ttaatctata aacctaatga tatctgccct
     2941 gagaacaaaa aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc
     3001 tgcgcagtac aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta
     3061 tcacagtgca taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct
     3121 aaagccggta acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca
     3181 gaacgtgact gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca
     3241 tgttatttac tatccaactt atcataatac taatatgtct gttttatggt gccagaaagg
     3301 gtggtatcgc gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc
     3361 accttcagct aggtaaacca ccagttgatg tcatgctggt tatcattgcg gtggtggcgg
     3421 catcggcgac cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc
     3481 tgctgcgccg caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga
     3541 ccattctttg cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg
     3601 ccattaagaa caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga
     3661 tggggattat cgccagtccg gtgtcggttg cggtcgtgtc tctggttgcg atgctgggta
     3721 atgtaacctt tgatggtcgc catcttgagt tcctcgatct gctggcaatc accattccat
     3781 cgacgttaat cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg
     3841 ataaagacga agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg
     3901 gtgataccgc gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga
     3961 ttttcctcgg ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat
     4021 ccttcggcgg caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg
     4081 gggcgctgat tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct
     4141 tccgttccgg tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gcagaaacca
     4201 tgttcggtgc gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc
     4261 cgtgggcgta tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg
     4321 cgctggcggc gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg
     4381 cttcagcacc ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag
     4441 cgattcagtt tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt
     4501 ttattctgcc ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg
     4561 cgatgtacgg gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat
     4621 tttcgaataa caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta
     4681 ccaggcacct ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta
     4741 cgttagcgtt gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagccctgac
     4801 cctgctggcg cagcaagcct ttcacgacgc ttctttcatg ctccgtccgg cacaccagaa
     4861 acaggttgcg gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcact
     4921 gcaattctta agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac
     4981 cggcaccgcg gtcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga
     5041 agaagcgctg tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctactcgca
     5101 aaacgcggcg ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat
     5161 cgacctgtac gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg
     5221 ctctgccaac aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact
     5281 gaaaaacttc ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca
     5341 tatcgcgttt gtgattggcg gtacgtctgc ggaaaccaac ctgaaaaccg tcaaattagc
     5401 aagcgctcac tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg
     5461 cgatgtccag ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca
     5521 gtttggcggt aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc
     5581 atcctgcccg gtaggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat
     5641 caaccgcgaa ggtatctgga tcgaaaaact ggaacacaat ctaggtcagt acattccaca
     5701 agaactgcgt caggccggtg aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa
     5761 agagatcctc gcccagcttt cgcaatatcc ggtttccact cgtttgtcgc tcactggcac
     5821 cattattgtg ggtcgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa
     5881 agaacttccg cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc
     5941 tgccggttat ccatcaggtt cacttggccc aaccaccgca ggtcgtatgg actcctatgt
     6001 ggatctgctg caatcccacg gcggcagcat gatcatgctg gcgaaaggca accgcagcca
     6061 gcaggttacc gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc
     6121 ggcggcggta ctggcgcagc agagcatcaa gcatctggaa tgcgtcgctt atccggagct
     6181 gggtatggaa gctatctgga aaattgaagt agaagatttc ccggcgttta tcctggtcga
     6241 tgacaaaggt aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa
     6301 gtaacctctt cggcccagcg cctggcagca tgctgcc
//
LOCUS       DAJTYF010000175         6314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTYF010000175 DAJTYF010000000
VERSION     DAJTYF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.176"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..385
                     /locus_tag="OO590_003849"
     CDS             <1..385
                     /locus_tag="OO590_003849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639018.1"
                     /translation="ENIIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLY
                     PVKITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITN
                     KKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(461..925)
                     /locus_tag="OO590_003850"
     CDS             complement(461..925)
                     /locus_tag="OO590_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6639019.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(922..1797)
                     /gene="nanK"
                     /locus_tag="OO590_003851"
     CDS             complement(922..1797)
                     /gene="nanK"
                     /locus_tag="OO590_003851"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6639020.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1794..2483)
                     /locus_tag="OO590_003852"
     CDS             complement(1794..2483)
                     /locus_tag="OO590_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6639021.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2531..4021)
                     /gene="nanT"
                     /locus_tag="OO590_003853"
     CDS             complement(2531..4021)
                     /gene="nanT"
                     /locus_tag="OO590_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6639022.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4131..5024)
                     /gene="nanA"
                     /locus_tag="OO590_003854"
     CDS             complement(4131..5024)
                     /gene="nanA"
                     /locus_tag="OO590_003854"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6639023.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5146..5937)
                     /gene="nanR"
                     /locus_tag="OO590_003855"
     CDS             complement(5146..5937)
                     /gene="nanR"
                     /locus_tag="OO590_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6639024.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1610 a         1706 c         1562 g         1436 t
ORIGIN      
        1 ggaaaatatc atcagatggc tgcttaaaaa aagaacaaat ggtagtgtcc gctattgcca
       61 gtacacctca aaagttgcca tgatttacat tgaacagctt gaattaatcc ataaaagtgg
      121 tgatgtgttg tatccagtaa aaataaccag aaaatcatca ggaaaaactg ccttccacct
      181 tgttccattc ggcttaaata agactcacga ccttctagag gtggaagatg cttcagaagc
      241 aattcggctg gtgattgatg aacgtcattc cattcgctgc agtacactta cagcaactat
      301 tacgaataaa aagggcaagc gaattaagcg gacaggtata tacagtatca agggtgttaa
      361 cataaaagaa tacaacgtca ggtaatggca cagagcggtc cgtcagttca ggtttggctc
      421 tgtgccaaag atgggtcagg tcacacctga tctaataaat ttaagccatt aaatcagcct
      481 taaccttcac caccaccttt ttaatctcac ccggctcgcc aacaacgcat cccggcttat
      541 gcggctctcc tggcataaac acggcgaaca ttcccggttt caggatgatg gtttgctcgt
      601 tttcaatggc gctacaaagc tggtaatcat cttcatggtg gaactcttca cattggcgcg
      661 cagtgcctgc catgccaaac agaatccgtt cctcaccgtt taataacagc tggatatcaa
      721 tgtattgctc gtgcaattcc gcttttttct cgacgggcga ttgagtgtta aacgtcatga
      781 cattcataaa aatattgtcg ccctgtaatt cgtaacgacc cggcgctttt tcttgcggtc
      841 tggcagctaa ggccagcgtt aacgcgtcct gtaacgcagg atgtaaccca gcagacggta
      901 atgactgtac ttcacccatc atcataattt ttctccctgg gccaacagcg cagccccaag
      961 taaacctgca tcatggcggt aatgcgccgc cagtaaatca acatgaaatg ccgctggctc
     1021 ctgcgccaga tacatttcca ccaacgccag atacccttct gccagaccaa cgctgccacc
     1081 aaccaccacg cactggcaat cagttgtggc tttaatatca gcgatcagcc ttgcaagcgt
     1141 acgtgcggag cgatgaatca gttgctgcgc ctgctcatcg ccctgcccgg cgcgtgtgaa
     1201 aatagttctc gcatccgcgc cagccagctc gccctgcgct gccgccgcaa tgccgcgacc
     1261 agaagcaatt gcttccacgc aacctgtgcg tccacagccg cagactgggc cgtgtggatc
     1321 ggcaagcgta tgcccgatgt gccccgccag accgccaggg ccggtaagca gtttgccgcc
     1381 gctcactaca ccgccgccga cgccagtgga aacggtgata aagaccatat cggttatatc
     1441 gccttccagc gcctgatact ctgcccatgc tgcggcctgc gcgtcgttaa tggcaatggt
     1501 cggcaaattg gtaagttgtt ccagcgtttt gactaacgga aagtgtagca atccaccgag
     1561 attatgcgga ttcagcgcca gcaaactgcc gtcgcgaata attccggttg aagcgatggc
     1621 aacccgctgc gcatgagctt gcaacggaga gactaatgcg gataaggcat cacgcaaggc
     1681 ttctggtgtc tggctggctg gcgtaggaag ttcacgacga tcgcggatct gcccgtcagc
     1741 gccaatcagc gcggcggcaa gtttagtacc gccgatatca atcgccagta tggtcatagc
     1801 accgcctttt tcatcgctgt gttgtaccac tgacaaatgt gttcaagacg cgtaatggca
     1861 gaaccgaccg tcaccgccca cgcgccgtgg cgcatcgcat ctgccgcctg agcaggcgtg
     1921 ttgtaacgcc cttcggcaat cacccgacat ccggcttcgc tcaacgtttt caccagcgcc
     1981 agatccggct cttctggcgt ttcaggcgtg gtatagccag aaagcgtagt gccaataatt
     2041 tcggctccca gcttttggca tgccaggccg tcttccggcg ttgagcagtc ggtcatcgcc
     2101 agtaaaccgt gatgatggat acgtgccagc agcgtttcaa caggcaccgg acgcgggcgg
     2161 tcggtgccgt caatggcgat aatgtccgcg cccgcctgcg ccagcgcatc gacatcttca
     2221 atataggccg tgatgcgtac cggagaatcc tccagatcgc gtttcacaat tccaataatc
     2281 ggcacgctca ccaccgcacg cgtggcttgc agatttgcca caccttcgat gcgaatggca
     2341 accgcgcccg cctgctctgc cgctaatgcc atggctgcga cgatttcggg tttatcgagc
     2401 gggctgtcag gaaccggctg gcaggagaca atcaggccac cgttagcagc gattttttga
     2461 tccagttgtg caagtaacga catacatctt cccttagcga aaggcccggt acatagaccg
     2521 ggcaacagga ttaacttttg gttttgacta aatcgttttt ggcgctgcca aatggcacgg
     2581 caccgctaaa tggtttgccg tcgatagcgt catgagtacg caatgcttcc gggcgcagcc
     2641 aacgctgaac gcgggaaggc atatccagac caatcagcag gatcaccaca aatgtcaggc
     2701 tgaacgagag cgatgccagc gcagtaccca gatccagacg ttgagcgatc aacgcgccga
     2761 tgattggggc cagtgcgcca cccaatgcgc caacgttgta ggtaaagccc aggcccgctg
     2821 cacgctggtc ggtatcgaaa taaccgccaa tcagtttcgg taagatcccg gcgatccctt
     2881 gtccaagcat ttgctggaag aacagtaaca gaccgagcac ccagacgttt gcgccgccaa
     2941 tcgcaaatac cggaataatc agcagctgcg aggccagcag gctacaaacg tacgctttgt
     3001 gggttcccag ccagtcaccg aggaagccac ctacgcagca tcccacggcc gcgccaaagc
     3061 cactaaagaa cagcacattg gcgacagtat gcgggttata agccagatcg gttttcagat
     3121 acgttggcag cagcgcctga atcggccatg agtagaggaa agcaaacaac acgaccacca
     3181 tcagcattac gcccgttggc cagcgtttac ccgtactttg caccataaag ctgataaaga
     3241 ttgcggcgca taacagcccg agaacagcga cgattgcagc attttgcagg ttaccggcga
     3301 agcagaacca cagcgcagta gccgccgcca gtgtcattac gatattggca atgcgatgtt
     3361 caccacggta gagaatatcc accattgtgc gtactggtgc tttacctgcg tgtttctctt
     3421 tccagtcttc cgcttccggg atgtttttac gcagccagag agcaaagatg attggcaaaa
     3481 tgccgataaa gaacagcgca cgccaacccc agaccggaac caccaggcta tagacctgag
     3541 cggcaacgac ggcccccaca gagaagcctg aaatcaaaaa accactggct ttgttacgca
     3601 gatgttttgg ccagctttca atgacatagg tggcgctgga accgtattca cccgccatcc
     3661 ccatgccgat gaccagacga gcgataaaca tggtgatgta gcctggcgca aagccgcagg
     3721 ccagcgtccc ggccgagaag agaacgatgc tggtgaccat tgccagacga cgcccgtagc
     3781 ggtcacccat agcgccgagc atcaggccgc cgaaccagcg agagataaag gctgcggaga
     3841 tcagacttgc cgcctgcacc gtcgtcagcc cgaattcacc ctgtacttcg gtgagtacca
     3901 gggcgattaa aacgaaatca aaaccgtcaa gcagatatcc caaccaggca gcggaaaatg
     3961 cgcgccattg tgcacggttg agatggcgat accacgggat attctgggtt gtagtactca
     4021 ttgtgagtct cccgcggtgg gcgatgccca cacgctttgg tatgaaaatt gtagggtaca
     4081 gatgcgttta tttcccctca cccggtaggg gcgagcgagg ggaaaacaac tcacccgcgc
     4141 tcttgcatca actgctgggc cagcgccttc agttctggca gatatttttc atctaccggt
     4201 ccaaacggtt tgcggcacag cggcacagaa acgacatcca tataatgcag gacagttttc
     4261 aggccgcgga atacgcccgt tttgatcagt aaatcaatga ctttgttgca ctcagtttgc
     4321 agtttctgcg cggtctggat atcgccttct ttcagcgcct tcacgatccc ctgatatcgc
     4381 cagcccatga tgttgtaagt actgccgata ccaccatcag cgcctgccag caagccggaa
     4441 gcgaagattt cgtcgtaacc gttatagagc accagatcag gatgttcacg acggatctgt
     4501 tccatctgat agagatcgcc agaagtctgt ttcagcgcac cgacgccagg caacgtaacc
     4561 agggtgttga tctgatccag ggtcagtttt accccactca gggctggaat gttgtacacc
     4621 accatcggca aaccatccgc tgaatcaata attgcccgat agtgatcgca gtgttcttca
     4681 aagctgaaag gatagtagaa cggcgtgacg gcggagacgg catcgaagcc ataacgttta
     4741 gccgatgccg caagttgttg gctttcagcg gtgctgacgc aaccgacgtg ggcgatgagt
     4801 ttaatcttac ctttcgcctc ttcggcgacg atttccagta cctgttcacg ctcggaaagg
     4861 ctttgtacaa aggcttcgcc ggtcgaacca cccacgtata aaccgtcgat gccctgctga
     4921 atattgaact gaaccaggcg gcgcagactc gctttatcca gtgcttgttg ttgatcaaaa
     4981 ggagtcagga gtgcagccat tacgccacgt aaattcgttg ccataaatac ctctgaagtg
     5041 atgcttgtct gataaacgat atacctttat acctgttata ccagatcaat taagcaacac
     5101 cccatacaga aagcttataa tgcgatctgc ttcactaaag tggcattatt tctttttgtt
     5161 ggtggtctga ccgaaagcgt gccaggtagc agagacgctg ttgagatgcg attgcaacgc
     5221 acgatcggct tcgtcaggat catgacggcg gatcgcatca acgatcgcaa tatgctgttg
     5281 ataactaacg ttgttatgtt cgtgcagtgc ctgatcggta accgttgggc gtgcggcaat
     5341 aagccagtcg agcagggcaa cgtggatcgc catgaagatt gggttaccgg ggatctccgc
     5401 cagcacgcgg tggaaatcaa cgtctgaacg aatgaatgcc gcgttgttat ccagcgactg
     5461 actgttgatt tccagtgctt ttgccagcaa atcgatttgc tcatcggtgg cgtgttcagc
     5521 cgcatagcgc accaggctgg attcaaagaa cagacgtaat tgttcgaaat gggcaatccc
     5581 accaggatga gaaaggaaat ctttcgccat gccggaaagc tcgccgatga tggtgtccgc
     5641 agaaggtcga gagacacgag cgcgttcgcc gttgtttatt tgcaccagac ctttgcgttt
     5701 taacgctgcc agcgcttcac gcacagaagg acgcccgacg ttaaagaacg ccatcagttc
     5761 gcgttcagac ggtaattgtt caccttcgcc aaattcacga cggcggatca tctgttccag
     5821 ctcttcttcc accatttcgg agagtttttt acgcgccagc gggcggctac gcaagttgcg
     5881 accaattgca ggtgaagaat cttcggtttg cgaatcaaat gcgttcataa ggcccattct
     5941 gtaaggtcag tgtgattaac atcatcagtg acatcctatc acaggattga aagtagggga
     6001 aaatggcagg tttttctctt tgtacctcat cattaccata attaacggaa taattaacta
     6061 ttgcgaaaaa ttaatgtaac gcagataaac acatcccgat tgaattattc ataagactat
     6121 tcacaagcat tatgaatatt atgaatgtgt tcttacaaaa taatcataat cgcatatttt
     6181 ttaatgaaaa atcacctcac ctacaattaa aaacacgaca tccgcaccat aaatagactt
     6241 gcaaaaaata taacatcatt gttttcaatc tgccatttat gggattgacc gttttctttt
     6301 gacacggagt tcaa
//
LOCUS       DAJTYF010000176         6173 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTYF010000176 DAJTYF010000000
VERSION     DAJTYF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.177"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            172..819
                     /gene="fucA"
                     /locus_tag="OO590_003856"
     CDS             172..819
                     /gene="fucA"
                     /locus_tag="OO590_003856"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6639025.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            847..1995
                     /gene="fucO"
                     /locus_tag="OO590_003857"
     CDS             847..1995
                     /gene="fucO"
                     /locus_tag="OO590_003857"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6639026.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2048..2803)
                     /gene="xni"
                     /locus_tag="OO590_003858"
     CDS             complement(2048..2803)
                     /gene="xni"
                     /locus_tag="OO590_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6639027.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTLAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2914..4281)
                     /gene="sdaB"
                     /locus_tag="OO590_003859"
     CDS             complement(2914..4281)
                     /gene="sdaB"
                     /locus_tag="OO590_003859"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6639028.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4339..5628)
                     /gene="sdaC"
                     /locus_tag="OO590_003860"
     CDS             complement(4339..5628)
                     /gene="sdaC"
                     /locus_tag="OO590_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6639029.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1571 a         1507 c         1645 g         1450 t
ORIGIN      
        1 aagagaactt gcagttattt actgtgatta gttgagctag gtcacaaaat ataagcttgt
       61 gaatttcttt cttgttaatc aggaagaaga tggtgggtaa ttaaacggct aattcaatag
      121 tgtgaaagga acaacattat tgccctgttt tgaatcagag agaggtagct aatggaacga
      181 aataaacttg ctcgtcagat tattgacact tgcctggaaa tgacccgcct gggactgaac
      241 caggggacag cggggaacgt cagtgtacgt tatcaggatg ggatgctgat tacgcctaca
      301 ggcattccat atgaaaaact gacggagtcg catattgtct ttattgatgg caacggtaaa
      361 catgaggaag gaaagctccc ctcaagcgaa tggcgtttcc atatggcagc ctatcaaagc
      421 agaccggatg ccaacgcggt tgttcacaat catgccgttc attgcacggc agtttccatt
      481 cttaaccgat cgatccccgc tattcactac atgattgcgg cggctggcgg taattctatt
      541 ccttgcgcgc cttatgcgac ctttggaaca cgcgaacttt ctgaacatgt tgcgctggct
      601 ctcaaaaatc gtaaggcaac tttgttacaa catcatgggc ttatcgcttg tgaggtgaat
      661 ctggaaaaag cgttatggct ggcgcatgaa gttgaagtgc tggcgcaact ttacctgacg
      721 accctggcga ttacggaccc ggtgccagtg ctgagcgatg aagagattgc cgtagtgctg
      781 gagaaattca aaacctatgg gttacggatt gaagagtaat ttcgtaaagc aacaaggaga
      841 aggatgatgg ctaacagaat gattctgaac gaaacggcat ggtttggtcg gggtgctgtt
      901 ggggctttaa ccgatgaggt gaaacgccgt ggttatcaga aggcgctgat cgtcaccgat
      961 aaaacgctgg tgcaatgcgg cgtggtggcg aaagtgaccg ataagatgga tgctgcaggg
     1021 ctggcatggg cgatttacga cggcgtagtg cccaacccaa caattactgt cgtcaaagaa
     1081 gggctcggtg tattccagaa tagcggcgcg gattacctga tcgctattgg tggtggttct
     1141 ccacaggata cttgtaaagc aattggcatt atcagcaaca acccggagtt tgccgatgtg
     1201 cgtagcctgg aagggctttc cccgaccaat aaacccagtg taccgattct ggcaatcccc
     1261 accacagcag gtactgcggc agaagtgacc attaactacg tgatcactga cgaagaaaaa
     1321 cggcgcaagt ttgtttgcgt tgatccgcat gatatcccgc aggtggcgtt tattgatgct
     1381 gacatgatgg atggtatgcc tccggcgctg aaagccgcga cgggtgtcga tgcgctcact
     1441 catgctattg aggggtatat tacccgtggc gcgtgggcac taaccgatgc actgcacatt
     1501 aaagcgattg aaatcattgc tggggcgctg cgaggatcgg ttgctggtga taaggatgcc
     1561 ggagaagaaa tggcgctcgg gcagtatgtt gcgggtatgg gcttctcgaa tgttgggtta
     1621 gggttggtgc atggtatggc gcatccactg ggcgcgtttt ataacactcc acacggtgtt
     1681 gcgaacgcca tcctgttacc gcatgtcatg cgctataacg ctgactttac cggtgagaag
     1741 taccgcgata tcgcgcgcgt tatgggcgtg aaagtggaag gtatgagcct ggaaggggcg
     1801 cgtaatgccg ctgttgaagc ggtgtttgcc ctcaaccgtg atgtcggtat tccgccacat
     1861 ttgcgtgatg ttggtgtacg caaggaagac atttcggcac tggctcaggc ggcactggat
     1921 gatgtttgta ccggtggcaa cccgcgtgaa gcaacgcttg aggatattgt agagctttac
     1981 cataccgcct ggtaaatgcg ctgatgtgat aatgccggat acgatttgcg gcgtatccgg
     2041 ctcgccgtta ccgtaccaac cgcaattgct gtaaattgcc gtcgatatgc aaatcggttt
     2101 gtaagcgggc aatatcgcgg cacagaaacg ccatctcttt atgggtttct aattttttgc
     2161 gccacttttc ggcaaccgca tccagattct catatatccc ttccagactc tgaaactcga
     2221 ccagcagctg cgtggcgctt tttggtccga ttcccgcaac acccggtacc tttgaactgc
     2281 tgatccccgc aagtccccag taatcgggca actgctgcgg ttgaacgcca aattctttat
     2341 cgataaatgg cgcatccagc caacgtttct ggaagtaatc acgaatacgt aatgtcggtg
     2401 aaagtaactg acagtagcct ttatctgtcg aaacaatcgt tgcctgatgc ccggccagtg
     2461 tcactttgac cgccagtgtg gcggctaagt catcggcttc gttgccgctg gctgaccagc
     2521 acgggacgcc gcgttgctca aaggcggcgc gtaatgcagg catctcgtcg tgcaactctt
     2581 ccggcattgg cggtcgatcc gctttgtaat ctggtaaacg ctgatgacgc cagccgctac
     2641 tgcggttttc atcatcaaaa acggcgaccg cgtgggttgg ctggctgtgc ataatgagct
     2701 gatcgagcgc atgctggcag gtttcgacac agggcgaacc ctgaacggca tgaatgcgac
     2761 ggataagatt cagtgcatcg acaataagca aatgaacagc cacgataacc cccttaaaga
     2821 tcaggcgata agggtaacat tcgatgccgg aacaggctat ggctggttga gaaaacagga
     2881 agaggcttcg caaaacgagg cctctgggag agattaatcg caggcaacga tcttcatcgc
     2941 caggccgccg cgagaggttt cgcggtactt ggcgttcatg tctttacctg tttcgtacat
     3001 ggtttcgata actttatcga ggcagacgcg cggttcgctg gtacggcgca gtgccatacg
     3061 cgcggcgttc accgctttca ctgctgcaat ggcgttacgc tcaatgcacg gtacctgaac
     3121 ctgccccgcg accgggtcac aagtcagacc gaggttgtgc tccatcgcga tttccgctgc
     3181 gatgcaaacc tgcgccgggc ttgcgcctaa cagttctgcc agaccagctg ccgccataga
     3241 gcacgccacg ccaacttcac cctggcaacc cacttcggca ccagaaatcg acgcgttcat
     3301 cttataaaga gaaccaatgg cgctggctac cagcaggtaa cgagccagtg agttagcgtt
     3361 cacttcgcgg ataaacttat cgtagtacgc cagcacagcc gggataatcc cgcacgcacc
     3421 gttagtcggc gcagtcacca cgcgaccgcc agcagcgttc tcttcgttca ctgccagtgc
     3481 aaacatgttg atccagtcaa caaccgccat cgggtcagtg gtggttttat cctggctgac
     3541 cagcatccgg cgtagtgccg cagcacggcg tggaacgcgc agtttgccag gcaacacgcc
     3601 ttcggtggaa ataccgcgct caataccgcc acgcatgact tcccagacgt tcgccaggtg
     3661 ctgttccagc tcttctttac tgtgcagcgc cagctcgttt ttcatcatca ggccagagag
     3721 tgacagcccg gtttcctgac aatgtttttg cagatcggct gctgaactgt acggataagg
     3781 aacttcaacc ggtgcgctat cctgctggcc aaaatgctct tcatcaacga taaagccacc
     3841 gccaatagag tagtaagtct ggctgtaaac gactttatcg cccgccagcg cggtaatgcg
     3901 cataccgttt tcatgcagag aaaggttgtc tgcatggaag ttcatgcact gatcaaccgg
     3961 gaactccact tcatgctgac cgtttgccag catcaggcga ccatgagtat tcacatcctg
     4021 aataaaaccg gggatggaat cgatatccac ggtatccggc aggttacccg ccaggcccat
     4081 aataatggag atatctgtgt ggtggccttt accagtcaga gagagcgagc cgtacacgtc
     4141 aaccaccacg cgggtcacgt ctttaagcag gttacgggca atcagatcgt cggtaaattg
     4201 tttacccgct ttcattggtc caacggtatg agaactggaa gggccaatgc cgattttgaa
     4261 aatatcgaat acgctaatca taggaaatac atcgcgttaa aacggaggaa gcgccgcccg
     4321 aaagcggcgc gaaaggactt agctgaacag agagtagaag attgcggaga ttgcaatcag
     4381 ccccatcacg acaacgaata cgttgctgat gtgaccgctg tacttacgca ttgccggtac
     4441 tttctgaatt gcgtacatcg gcatcaggaa caggatcatc gcgatgattg ggccgcccag
     4501 ggtttcaatc atacccagga tgctcgggtt cagggtggca acaatccagg tcgttaccag
     4561 catgaacagc gcagtgatac ggttcagctt gttgatttcg atagacttac ctttaccacg
     4621 cagagattta atcaccatac cgttgaagcc ttcacgtgcg cccaggtagt ggccgaggaa
     4681 ggatttggtg atagcgataa tcgcgataat cggagccatc cacgcgataa ccggtgcgtt
     4741 aaagtggtta gccaggtaag acagaatcga gatgttctgc tctttagccg cagccaggtc
     4801 tgccggagtc aggctcagta cgcagctgaa gacgaagaac attacggtca gcaccatcat
     4861 gatgtgtgcg aatgccagga tcttcgagca tttctgttct gccatatcgc cgtactcttc
     4921 acgcttcgca acggcgaaag aagagatgat cggagagtgg ttgaacgaga acaccattac
     4981 cggaattgcc agccacaggg tcatccacag accgtttccg gttgcagatg cagtgtccag
     5041 agacagcgtt tccagtgctg cgccgttcca ctgcgggatc aggtacagag ccagcagcat
     5101 cagtacgcca acaaacggga ataccagaat actcatcgct ttaacgatca tctgctcacc
     5161 aaagcgaacg atggtcatca tacccacgat caggatcagc gacagaatcg cacgcggcgg
     5221 tggcgtcata cccagctggt gagacatgaa gctttcaacg gtattggtga ttgccacgct
     5281 ataaaccagc aggatcgggt agatagcgaa gaagtagagc agggtaatca gtttacctgc
     5341 gccaatacca aagtgttctt ctacaacctc ggtgatgtct tcgcccgggt ttttaccaga
     5401 cagtacgaag cgagtcaggc cgcggtgagc aaaaaacgtc atcgggaacg caaggatagc
     5461 catgatgatc agcgggatca taccgccaac accggcgttg attggcagga acagcacgcc
     5521 cgcgccgatt gccgtgccgt aaaggcccag catccacatg gtgtctgtct tgcgccaggc
     5581 actacgagag tctttcgacg caatcgtgct ggtttgagtc gtttccatct atttctcctg
     5641 gaggaatgca aataattcag cttttagcca gttcagcccg ggaaggtgcc tgaccgtaaa
     5701 aatgaaatct gtctatcggc gatggcttaa tcatttaccg ctgtaataat ttgagggcgg
     5761 aaagatacac acaagttatg tatctatcag tgatcttgat cccaaataca ataatctgct
     5821 tggaagatag attatcccaa cgctttactc tgtaaatcaa aagtaaaaga gaatttaagg
     5881 gcaatatttg cccattaaac gacatctgac ttcttccggg gcgcgcaata tatgtaggtt
     5941 gaactgaaaa aatcaatact tttagttaat catggcgata atttcttttt catagcgata
     6001 aaatacggtt tatgaaaata gggaaacgtt tgcgttgcaa cataaattgt atggcatatt
     6061 aactgtgatg atatttgtcg cgtgagggtg gtaagaaaca ttcatataac aaatgaaatg
     6121 agttgatgag taagtgacca aaaacgagaa aacccggcaa caaacgtcac cgg
//
LOCUS       DAJTYF010000177         6152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTYF010000177 DAJTYF010000000
VERSION     DAJTYF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.178"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..113
                     /locus_tag="OO590_003861"
                     /pseudo
     CDS             1..113
                     /locus_tag="OO590_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            639..1817
                     /gene="ltrA"
                     /locus_tag="OO590_003862"
     CDS             639..1817
                     /gene="ltrA"
                     /locus_tag="OO590_003862"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6639030.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDVTRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1900..2079
                     /locus_tag="OO590_003863"
                     /pseudo
     CDS             1900..2079
                     /locus_tag="OO590_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2109..2591)
                     /locus_tag="OO590_003864"
                     /pseudo
     CDS             complement(2109..2591)
                     /locus_tag="OO590_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2847..3827)
                     /locus_tag="OO590_003865"
     CDS             complement(2847..3827)
                     /locus_tag="OO590_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6639031.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3827..5026)
                     /locus_tag="OO590_003866"
     CDS             complement(3827..5026)
                     /locus_tag="OO590_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6639032.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5348..5686)
                     /locus_tag="OO590_003867"
                     /pseudo
     CDS             complement(5348..5686)
                     /locus_tag="OO590_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5753..6152)
                     /gene="istB"
                     /locus_tag="OO590_003868"
                     /pseudo
     CDS             complement(5753..6152)
                     /gene="istB"
                     /locus_tag="OO590_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT         1668 a         1413 c         1467 g         1604 t
ORIGIN      
        1 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
       61 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      121 aagttatcca tgctctgagg tgggtggagg tgggccctcc ggtcagggtt tacaggagca
      181 ctgaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      241 aaggcagaga tctactgtca cgtggggttc gggaagctga acgaaagtaa actgtccgat
      301 gaagcatcga aatcttagat gatgccaaaa tggggccgta cagccgtccc ctgataagcg
      361 cagaagacac ctgcttactg actgcgcggc atccggtgtg aaggcagcag gatcccgggc
      421 cagaccatct cagggaacgt gagaacctgt ttccggcacg tatcgggaaa caggggcgga
      481 cccattcgta gtagtgatga aagatctgta atgggtctgg agcgaaggga tggggatcac
      541 attcattgca gaagacacaa cccaaaaggg gatgacgtaa ctgatgcaac gaaaatcatt
      601 tgaaatacca aaagcactgg tctgggcatc atatctggat gtgcgtcgca acaagggagc
      661 cccccggatg cgacgggcag actttgaaaa tgttcgacca gcaacgtgat ggcaatctat
      721 acaagatctg gaaccggcta tgttcgggaa catggtttcc gccaccagtg ctggaaaaac
      781 ggatcccgaa gtcgaatggc aaggaacgca ttctggggat cccaacagta tccgatcgaa
      841 ttgctcaggg agcgataaaa cttttcatgg aagaaaagct tgacccgatt ttccatgctg
      901 attcatatgg ttaccgacca ggcaaatcag ctcatgacgc tctgaaacaa tgtgccatcc
      961 ggtgctggcg ttatagctgg atactggaag tggacatcag tgcgtttttc gaccatgtga
     1021 gacatgatct ggtgctcaaa gctctggaac accacgggat gcctaaatgg gtcatcctgt
     1081 actgtcgacg ctggatggaa gcgccaatgc agagttgtga aaatggagaa ttaataaccc
     1141 gaacacgagg cactccgcag ggcggggtca ttagtccgct acttgctaac ttgttccttc
     1201 actatgcatt tgatttgtgg atggaaagag aatatcgggg ggtaccgttt gagaggtacg
     1261 ctgacgatat tgtagtacat tgttcacgaa tgagtgatgt aacaaggcta aagaacaggt
     1321 tatcggagcg cttctcggaa gtcgggctgg tcctgaatgc agggaaaacg aatatcgcct
     1381 acattgacac gtttaaaagg agaaacgtcg caacgagttt cacctttctc ggatatgact
     1441 tcaaagtgcg tacgctgaag aatttcaaag gcgaacggta ccgaaaatgc atgccgggtg
     1501 cgtcaaatgc agcaatgcgc aaaataacag aaacaatcaa gaagtggcgt atacatcgct
     1561 caacagctga gagtttgctg gattttgcga ggcgctacaa tgcgatagtg agaggctgga
     1621 tcgagtacta cggaaagttc tggtccagaa atttcaacta tcgactgtgg agtgcaatgc
     1681 agtcacgtct gctcaagtgg atgcagtcta aatacagact ttcgaaccgg aaggctcagc
     1741 gaaagctgac gctggtaagg aaggagtatc cgaagctatt tgttcactgg tatttactac
     1801 gtgcatcaaa tgagtgatca agagccgtat gacgcgagag tgtcacgtac ggttccgtga
     1861 gcagccgggg agtgaaattc tctccggctg actcgactcg cgggttccga cagcgatgac
     1921 gaacatgcgg cggtgttgta ctcgctgatc ggcacatgcc gtctgaacaa tgtggaacca
     1981 gaaaaatggc tgcgttacgt cattgagcat atccaggact ggccggcaaa ccgggtacgt
     2041 gatctgttgc cctggaaagt tgatctgagc tctcagtaaa tatcaatacg gttctgacga
     2101 gccgcttact tttggcgtaa ttctgctgcg cctttactga acacaatgcc gtattcagca
     2161 agaagtccac gaatgtgatt tatcagttgt gtcctttcca ttgaaaccaa ccgacgcata
     2221 ttcaaaaaag tctggacgtc ctgttgctcc agagttttta ttggtacggg tcgtatagtt
     2281 ccatgttggc aggcctcagc tattgcctgt gcgtcgttat aatcattttt ctggcaccgc
     2341 tgataagctt taacatgttg tgccggaagc aatattacat catggtcgag tttcctgatt
     2401 tcccgggccc agtaatgaga tgccccgcca gcctccatcg caacagtcat tttaggctgt
     2461 tgcacctgcc agttaagcag ttgattgcga gaaagtttgg tctttcctgc gggtttgcca
     2521 tgagcattga tactgaaaag ataaaagttc gttttagcca gatcaatgcc ggtgattttc
     2581 tgttcagaca tggtatcccg cttacttttg tggttaaagt ttatccatca tggcgcaatt
     2641 gaaggctgcc gtgaaagagg gggaagacta tctcatcggt tactgttaaa cggctaagag
     2701 aaactatctt tttcaagcaa agaagatacg gtgaaacagg cgatttcacc gtgaaatact
     2761 gttcaaaaaa aacgctaata aaagcaaaat ttcttttaaa acagccaatt aaaaactggt
     2821 gagttgctgg cgaaaaactt gaattttcac agcgaattca gattatcttt tagtacaaga
     2881 gcaatcatac gatctaactt atcctgaact tccaacggta atctgccgaa ttcatatgag
     2941 aacgtacgtc cctttactcg ttttcgagca aatttatctt tactatcaaa tttccataac
     3001 aaagttacaa ccgctttatc ctttactcct gaatcaagca atgaagacat ttccttggtt
     3061 ataagccgca taatactgtt tttaacctcg tcttcagaaa gattgtcatc agcagtaata
     3121 agctcaatac tcggcgagat attcttcaca acttcatcaa tagatatatc acccagcctc
     3181 agtcgttcac ttaattcagc cagttgcctg taatcggcgt atgtcagctc agactgaaca
     3241 ggaaaaagag acacaaaatc tttaggtacg gacgctgctt ggagggcacg agtcacctta
     3301 gctgccgaca atccctctct ttccgcaatt tgcttctggt tcataccggc ctctttgaga
     3361 cgtactaaac gcaacccaat ttcacgaata ttatgttcaa gtgacgtctg caaatctttc
     3421 gccagatgct gagcttcaga aacagtaagc tcatcatccg ttacaagtac acgtaatcca
     3481 accttgcata gcaaagctgc tgcccgacga cgggaaccat ccaaaatctc aataagatcg
     3541 ccagtccgaa tcccaataca tggatagaat tgttgtaaac gtatagttct ggtgatgtct
     3601 tttagtgagt ccggcgtcag ggctgtctgt tcgcggccat tcacctcttc aaccacaaaa
     3661 gtttgagtgt ccactttgtc atgatcaacc ttgatctcag taaattttgc ctttcggcca
     3721 gtattgaggg taaatacatg aactggagcc gatatttctt cagtattatt caggatattt
     3781 gtattcagcg tacggccaat agtcggtcgg tgctttctgt tttccatcag tagttggccc
     3841 ttataaattc aatacgatca aaaactgctt tagcgaaatc ttctgctgcc atacgtgcat
     3901 ttttcaaagc ttcaccactg ccaacataag taacaggatt cgcagatatc acggtatcaa
     3961 acgactcgcc gcttctctca aaaccatcaa gacgtggcat tgaaacatca agcatgtcac
     4021 caccgaaaat ctcttttgtc aggctatggc agtatttatg atctgactta ttcgccagtt
     4081 tcgacatgaa gccaatattt gcctgcaaac ggcatgaaca tccatcctgt tcaataatct
     4141 gtacaagttc aggtaaacga gccaaatatt taagcgtgga atggaaatca acttgtgctg
     4201 gcggcaccgg tgtaaacatg atatctgcgg cagcaatggc atttttcaaa aatgcgtcca
     4261 gatgcgggcc tgtatcaata aggatgaagt cgaaatcatg cttcagctta tcaataatat
     4321 tctcacgcaa tatcgcgtgt ttgttttgtc ctaatagatg ctcctcgcac agagtatccc
     4381 agtttgatgc aataaaggcg tcgtcaatag aagccggcat aacataaacg ccagggataa
     4441 ctgacggaac gataaaatct tccagtaatt cctcacgact cacgttctgc aacatagcct
     4501 gcggtgcggt tgtatcaacc agccccaccg catgcaagta gttcagaaac atagtggctg
     4561 atgactgggg atctaaatca aggagcagaa tacgtaaatc ttctgacagc aggtgaggat
     4621 gagcacgcag ggcgtgagct acggaaacac tactcacggt tttagacaca ccgcctttta
     4681 gactaccgat gaatatggaa tatgcctcac tataacgatc gcgatattta ggaatgccac
     4741 gatgtgcata gatatcgata atgttctgga tggttaatgc atattttgtc gcggttcctg
     4801 ctgggcgttt ttcaaactga tagccctgag cctccatctc accgactgca ccatccacgc
     4861 ttgcgcgcgt taattttgga agcttagcta cagcagactt tgaatagact tgataaaaaa
     4921 catcagcctg aaactcctgc ttttgctcct gaatctgttt tgttagggcg agcaacattt
     4981 tatctgcacg ctgcgcaacc ttacttaact gctcaaaact agtcattccc cctccgtgct
     5041 atgtaggatt ttaacttatt acaaaaaaaa ctgcattagc agggtaatgc atgcaagtac
     5101 atattgtaca tgcttatatt aaaaatgcgc gcattgctga tttattaatc atcggaatga
     5161 atattactgt aagatagtat gcctttccgg gagaaagacg ctgatttccc ccgaataatc
     5221 cagttcacca ccacactccg gacaacagct ttcttcaggc tccagatggt gtatttcgcg
     5281 ggaaacatgt tccggtaacg gacggcgatg acgtgactgt cgcaacaggc gggggtagtg
     5341 cttccaatca gtagaacatg tgcttttcaa tgaaggagcc gatgactttt tcgtggattt
     5401 ccacggaacg cgagaagcag attgttttac gtgccagccg cttgatgcgg gtgcagaggg
     5461 tccggttgtt acgttcaata cgttgcgtga agattttgcc ggtcaggtgt ttctccttcg
     5521 gcacttctct ggcgtagctg ccccagtcat cgctggtcac catgccgata cagaacggag
     5581 tcaacagggc taacagttcc cgacaggtct catcattccg tggcccgaaa gtgtaagcca
     5641 acacaccgcc agttttagtg ttgtacgcat accagagcca gtgttgtgtc aacgacggat
     5701 gaaaagtgct ccacttatat ctccaccaac ggcccaatat tgatccaccg ttttactcag
     5761 gattagcttc tgctataacc ccggcctttc gtttctgtct gagtcgatag ctttctcctt
     5821 tgatttgaac gacatgtgag tggtgtaaga tacggcccag catcgctgag gtcagtgctg
     5881 catcaccggc gaacgtttga tcccactgcc cgaacggtag attggatgtc aggatcattg
     5941 cgctcttttc gtaacgttta gcgatgacct ggaagaacag ttttgcttct tcctgactga
     6001 acggcagata gcctatttca tcaatgatga gcaggcgggg gccattactc cacgctgaag
     6061 cgtcgtttta taacggccct gacgttgtgc cgtagataac tgaagtaaca gatctgctgc
     6121 tgttgtgaag cgaactttga tacctgcacg ga
//
LOCUS       DAJTYF010000178         6036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTYF010000178 DAJTYF010000000
VERSION     DAJTYF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.179"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(127..276)
                     /locus_tag="OO590_003869"
     CDS             complement(127..276)
                     /locus_tag="OO590_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639033.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            1465..2280
                     /gene="sul2"
                     /locus_tag="OO590_003870"
     CDS             1465..2280
                     /gene="sul2"
                     /locus_tag="OO590_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6639034.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2367..2669
                     /locus_tag="OO590_003871"
     CDS             2367..2669
                     /locus_tag="OO590_003871"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639035.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2845..4338)
                     /locus_tag="OO590_003872"
     CDS             complement(2845..4338)
                     /locus_tag="OO590_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCR6639036.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(4382..4771)
                     /locus_tag="OO590_003873"
     CDS             complement(4382..4771)
                     /locus_tag="OO590_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639037.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4989..5429
                     /locus_tag="OO590_003874"
     CDS             4989..5429
                     /locus_tag="OO590_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6639038.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1434 a         1664 c         1583 g         1355 t
ORIGIN      
        1 aaaaaaaagg aatatttcgt tttcacgagt aatacccggg cattaaaaat ccactgtaat
       61 tacatgaaaa taaagaaata aaaggtaata ccggagtatt accacgtacg cgggcaagcg
      121 aaaaagctat aacgcttccc ggccgacagg cgacccaaaa tccacagggg catgcaggtt
      181 ctcttcagta acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc
      241 agtcgaatcc agttgttcat catattcaga tggcatgggg tgatatccag tagctgagag
      301 gttgttagcg ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat
      361 actggcttac tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga
      421 aaaaaggcag caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg
      481 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg
      541 ggacggaaat ttcctagaag ataccaggaa gacacttagc aaagaagaga taaggccacg
      601 gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcgcaaaat ctgacgctca
      661 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
      721 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      781 ccgcggtttc tcattccacg cctgacactc agttccgtga aggcagttcg ctccaagctg
      841 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa
      901 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat tagtaacagg
      961 agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg gctacactga
     1021 aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag cagttcccca
     1081 actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga
     1141 gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta tcaattaatc
     1201 ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa caggtttccc
     1261 tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct acttattgag
     1321 agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg atcaccgcaa
     1381 acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga ctcatgctcg
     1441 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
     1501 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
     1561 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
     1621 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
     1681 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
     1741 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
     1801 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
     1861 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
     1921 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
     1981 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
     2041 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
     2101 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
     2161 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
     2221 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
     2281 ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc ctttgagggc
     2341 gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca gacggtattc
     2401 gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg
     2461 ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc
     2521 gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg atgggcatgg
     2581 acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc ccttttcacc
     2641 caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat gcaacccaat
     2701 ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca ctagcggctc
     2761 cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca gcattttgat
     2821 tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct cagccggtgg
     2881 cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct tcaggtgatc
     2941 aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca tggcgccgcc
     3001 gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt gcgcccatgt
     3061 catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga ccttgttgcc
     3121 cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg cgaacacccc
     3181 gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc ttgcaatgaa
     3241 atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct tgagctggta
     3301 gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg ggcggctgat
     3361 gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca cgccggcgtg
     3421 caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac tggtcggcag
     3481 agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac ggtaagtgat
     3541 cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg ccagatagct
     3601 gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca cccggtgggc
     3661 gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc agcggaaacg
     3721 cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt ggaagtgaac
     3781 attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg tcttggccac
     3841 ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt aaacgatgcc
     3901 cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct gaaacgggaa
     3961 gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca gcaaggcggc
     4021 actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct tacagctgaa
     4081 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
     4141 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
     4201 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
     4261 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
     4321 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
     4381 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc
     4441 cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg ttattgcgtg
     4501 caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa ttaatattct
     4561 tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat gcagtataag
     4621 atgaaccact actattatca tggccaccat taccaccacc gttaaaacta cctcccgaac
     4681 ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac
     4741 cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact tttaaaaaca
     4801 gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact acgcccgtca
     4861 aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca gcctcagacc
     4921 ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca ggagacactg
     4981 tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga aagtcgcgct
     5041 cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc
     5101 gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc acttctgaga
     5161 caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg gaaaatgctc
     5221 agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc ctaatgcttg
     5281 aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac
     5341 aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg ggattctggt
     5401 cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt accataacat
     5461 ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac cggtaaaaca
     5521 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     5581 gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg taagtcctgt
     5641 ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     5701 aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta
     5761 caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa aagtgatgta
     5821 aaagtgatgt atgaactgaa ggagcgatgt aattctgtag tcaggaagaa taacccgttc
     5881 atgcaggcat gaacgataca gtgaggaacg ctttttttgt cacgatctcc ccttaacctg
     5941 cgtaaatcac ccgccatggt tgattatttt tcaacctcaa aatggtcata cacctaacgc
     6001 agccgcgatt ttcatcgcaa ctgcatgaat taaaag
//
LOCUS       DAJTYF010000179         5999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTYF010000179 DAJTYF010000000
VERSION     DAJTYF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.180"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..270)
                     /locus_tag="OO590_003875"
     CDS             complement(<1..270)
                     /locus_tag="OO590_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6639039.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEP"
     gene            807..2708
                     /gene="mtlA"
                     /locus_tag="OO590_003876"
     CDS             807..2708
                     /gene="mtlA"
                     /locus_tag="OO590_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6639040.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2803..3951
                     /gene="mtlD"
                     /locus_tag="OO590_003877"
     CDS             2803..3951
                     /gene="mtlD"
                     /locus_tag="OO590_003877"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6639041.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3951..4538
                     /gene="mtlR"
                     /locus_tag="OO590_003878"
     CDS             3951..4538
                     /gene="mtlR"
                     /locus_tag="OO590_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6639042.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4550..4759)
                     /locus_tag="OO590_003879"
     CDS             complement(4550..4759)
                     /locus_tag="OO590_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639043.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5043..5405
                     /locus_tag="OO590_003880"
     CDS             5043..5405
                     /locus_tag="OO590_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6639044.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1567 a         1455 c         1489 g         1488 t
ORIGIN      
        1 cggttcagtc gctttctcct gcgacgcaac atgtgactgc ataccccagc cacgctccgg
       61 ttgatagagc gtcgcccaga tccacagaaa cggccagata acatgcagag taaacaggct
      121 cacccagccc gccgtatgaa tagcgtcggc atgggggtga ttgcgctttt tggcaatcag
      181 ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag
      241 cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt tatcagtgtt
      301 gttttttggc tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg
      361 cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct tagttacata
      421 acgattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa
      481 atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa tgtgacatta
      541 ctcacattta aatgccattt ttagcgaaaa tcaccgcctt gtttcttttt tacacaggcg
      601 ttttgtgatg agcgtcacgt caattacctc tctaccccct atatttatgt gactgatatc
      661 acacaaaagc ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac
      721 aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga
      781 cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct
      841 ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta
      901 tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg
      961 tcgggccgat gatcacttat ctcctaccgc tgctgatcgg ttataccggt ggtaagctgg
     1021 taggcggcaa acgtggcggc gtagtcggtg ccattaccac catgggcgtt atcgtcggcg
     1081 cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta
     1141 agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata
     1201 acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc ggcattggcc
     1261 cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg
     1321 acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc ctcaacaacg
     1381 ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa ctgggtaaat
     1441 caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg ctggcgtaca
     1501 tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc atccacttcc
     1561 tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt ctgatcctcg
     1621 cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg
     1681 tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca aaaggtgctt
     1741 atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcta
     1801 ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta
     1861 tgcaggacat gaaagctgag tctaaaggca catctccgct atctgcgggc aatgtgacta
     1921 acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg ggttcaagtg
     1981 cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtcg cagatttcgg
     2041 tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc actcaccgtg
     2101 acctgaccga acgcgctatg cgccaggttc cgcaggcaca gcatatttcg ctgaccaact
     2161 tcctcgacag cggcctgtac accagcctaa ccgaacgtct cgttgctgcc cagcgccata
     2221 ctgaaaacga agtgaaagtg aaagacagct ttgacgattc cagtgctaac ctgttcaaac
     2281 tgggcgcgga gaacatcttc ctcggtcgca aagcagcaac caaagaagaa gcgattcgtt
     2341 ttgctggcga gcagctggtg aaaggcggtt acgttgagcc ggaatacgtt caggcgatgc
     2401 tggatcgtga aaaactgacc ccgacttatc tgggtgagtc tatcgcggtg ccacacggta
     2461 cggttgaagc gaaagatcgc gtactgaaaa cgggcgtggt gttctgccag tacccggaag
     2521 gcgtgcgctt cggtgaagaa gaagatgaca ttgcccgtct ggtgattggt attgcagccc
     2581 gtaacaacga gcacattcag gttatcacca gcctgaccaa tgcactggat gatgagtccg
     2641 tcatcgagcg tctggcacac accaccagcg tggatgaagt gctggaactg ctggcaggtc
     2701 gtaagtaatc caatcctacc ctctcctcac ggagagggtt tgggtgaggg aaaagcctca
     2761 ccccagccct ctcgggtaaa aacattgatg aaggttaata ctatgaaagc attacatttt
     2821 ggcgcaggta atatcggtcg tggctttatc ggtaaactgc tggcagacgc gggtatccaa
     2881 ctgacgtttg ccgatgtcaa tcaggtggta cttgatgccc tgaatgcccg tcatagctat
     2941 caggttcatg tggtcggtga aaccgagcag gtagataccg tttccggcgt caatgctgtc
     3001 agcagcattg gtgatgatgt cgttgatctg attgctcagg tcgatttagt cactaccgcc
     3061 gttggcccgg ttgtgctgga acgtattgct ccggctatcg ccaaagggct ggtgaaacgc
     3121 aaagaacaag gtaatgaatc cccgctgaac atcatcgcct gtgaaaacat ggtacgcggc
     3181 accacgcagc tgaaaggtca tgtgatgaac gccctgccgg aagacgccaa agcgtgggta
     3241 gaagagcacg ttggctttgt cgattccgcc gttgaccgca tcgtaccgcc ttcggcttcg
     3301 gcaactaacg atccgctgga agtgacggta gaaactttca gcgaatggat tgtcgataaa
     3361 acgcagttca aaggcgcact gccgaacatc ccaggcatgg agttaaccga caacctgatg
     3421 gcatttgtcg aacgtaaact cttcaccctg aacacgggtc atgctataac cgcgtacctc
     3481 ggaaaactgg ccggtcatca gaccattcgt gacgctattc tcgacgagaa aatccgcgcg
     3541 gtggtaaaag gtgcgatgga agaaagtggt gcggtactga tcaagcgcta cggctttgac
     3601 gcagacaaac atgcggcgta catccagaaa atcctcggtc gttttgagaa cccgtatctg
     3661 aaagatgatg tagagcgcgt aggccgtcag ccgctgcgta aactgagtgc tggcgaccgt
     3721 ctgatcaagc cactgctcgg tacgctggaa tacagccttc cgcacaagaa tctgattcag
     3781 gggattgctg gtgcaatgca cttccgcagt gaagatgatc cgcaggctca ggaactggca
     3841 gcactgatcg ctgacaaagg tccgcaggcg gcgctggcac agatttccgg tcttgatgcc
     3901 aacagcgagg ttgtatccga ggcggtaacc gcttataaag caatgcaata atggtggacc
     3961 aggcgcagga caccctgcgc ccgaataaca gattgtcaga tatgcaggca acaatggaac
     4021 aaacccaggc ctttgaaaac cgtgtgcttg agcgtctgaa tgctggcaaa accgtgcgaa
     4081 gctttctgat caccgccgtc gagcttttga ccgaggcggt aaatcttctg gtgcttcagg
     4141 tattccgcaa agacgattac gcggtgaagt atgctgtaga accgttactc gacggcgacg
     4201 gtccgctggg cgatctttct gtgcgtttaa aactcattta cgggttgggc gtcattaacc
     4261 gccaggaata cgaagatgcg gaactgctga tggcattgcg tgaagagcta aatcacgacg
     4321 gcaacgagta cgcctttacc gacgacgaaa tccttggacc ctttggtgaa ctgcactgcg
     4381 tggcggcgtt accaccgccg ccacagtttg aaccagcaga ctccagtttg tatgcaatgc
     4441 aaattcagcg ctatcaacag gctgtgcgat caacaatggt cctttcactg actgagctga
     4501 tttccaaaat cagcttaaaa aaagcctttc aaaagtaagc aacgtctgct tactgcccct
     4561 ctacctgctt cggccgataa agccgacgat aatactccag acgttgtaga tataacggca
     4621 cgctttcatc gggtattccg gacggaatcg cgttacgggg aggaagtttt ttcagatact
     4681 cccggaacgc ctggcttgat gccatgaaat ctacggcttt atcgataaga agcggaacgt
     4741 tttcacctaa tttgcccatg atgatatctc cgtattaccc cgcgccggga atgcgcggcc
     4801 gccaatttta gtttaggttc ccggtaaaga tgacattaag gaaaacgtgc tgaatcctca
     4861 ataatcatcc gctgctgttt attatctttt tctatagatt tcttaattaa tcaacgaatt
     4921 gcgtagtttt tgagcatgtt aatgattacg ttgcagaact attacaatgt catcagttgt
     4981 gaacaaagca cctggtcgcg catactaggg gctataaatt tatctttatc agaagccatc
     5041 acatgaaaga agtcgaaaaa aacgaaatca aacgtctcag cgatcgcctg gacgccatcc
     5101 gccaccagca ggccgatctg tcgctggttg aagccgcaga caaatatgcc gagctggaaa
     5161 aagagaaagc cacgctggaa gcagaaattg ctcgcctgcg tgaggttcat agccaaaaac
     5221 tgagtaaaga agcacagaag ctgatgaaga tgccgttcca gcgagcaatt accaaaaaag
     5281 agcaggctga tatgggcaag ctgaagaaaa gtgttcgcgg actggtggtt gtgcacccaa
     5341 tgaccgcgct ggggcgcgaa atgggcctgg aagagatgac tggattttct aagactacgt
     5401 tttaagcccc tcagacgact ggttcgatcg tatcaacccg catctggctt gccttagctg
     5461 gtcaatgcgg ggactctcct gtctactcgc ctaattacca aacaattcac tccctttact
     5521 tcgatttacc gctggttgat attctgaaaa tgcattttta ccgaaatagc catctataaa
     5581 caataaaaat atatagaaga tatcatttgt taattaacaa gcatttacgt ctcttttttt
     5641 cggatttaat tgtgaaattt taatcatcat attcacaaaa tcaattatct gtaaaatttt
     5701 tcccagacat ggtttctttc atatttgaca tttattaata atcatcatta gaatccacga
     5761 aaaataatat attcatacaa acacagacat cttttgaaac gaatgataca tttttatgat
     5821 gaatgcttaa aacaatattt aatcgacaca catcactgtc attaatgtaa agcaacatag
     5881 ctcaccaata atacaatgga caactctacc ggcattgatc gctggtcgga ggaaataagg
     5941 atttttacat gaacttgaag aagatttttt tcagtgctgt cactgtttca gttctctgc
//
LOCUS       DAJTYF010000180         5775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTYF010000180 DAJTYF010000000
VERSION     DAJTYF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.181"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            121..639
                     /gene="mddA"
                     /locus_tag="OO590_003881"
     CDS             121..639
                     /gene="mddA"
                     /locus_tag="OO590_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6639045.1"
                     /translation="MSIRFARKADCAAIVEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            636..1085
                     /locus_tag="OO590_003882"
     CDS             636..1085
                     /locus_tag="OO590_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6639046.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1086..1318)
                     /locus_tag="OO590_003883"
                     /pseudo
     CDS             complement(1086..1318)
                     /locus_tag="OO590_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1404..1577)
                     /gene="ortT"
                     /locus_tag="OO590_003884"
     CDS             complement(1404..1577)
                     /gene="ortT"
                     /locus_tag="OO590_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6639047.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1772..1867
                     /gene="yncL"
                     /locus_tag="OO590_003885"
     CDS             1772..1867
                     /gene="yncL"
                     /locus_tag="OO590_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6639048.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2269..3078)
                     /locus_tag="OO590_003886"
     CDS             complement(2269..3078)
                     /locus_tag="OO590_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR6639049.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3288..4713)
                     /gene="patD"
                     /locus_tag="OO590_003887"
                     /pseudo
     CDS             complement(3288..4713)
                     /gene="patD"
                     /locus_tag="OO590_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4735..5441)
                     /locus_tag="OO590_003888"
                     /pseudo
     CDS             complement(4735..5441)
                     /locus_tag="OO590_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(5431..>5775)
                     /locus_tag="OO590_003889"
     CDS             complement(5431..>5775)
                     /locus_tag="OO590_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6639050.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1512 a         1454 c         1284 g         1525 t
ORIGIN      
        1 ctatcactta aataagtgat actggttgtc tggagattca gggggccagt ctatccgttg
       61 cttcgggctg ttttcgatag tattgataac atcatcatca ttattttatt gaggccagac
      121 atgtccatcc gttttgcccg caaagccgac tgtgctgcca ttgtggaaat ttataaccac
      181 gccgtgttgt atacggcggc tatctggaat gaccaaacgg tggatgcaga taaccgcatt
      241 gcctggtttg aagcacggac catagcaggt tatccggtgc tggtaagtga agaagatggc
      301 gtagttacgg gatatgcctc gtttggcgac tggcgtagtt tcgatggttt tcgccatacc
      361 gtggaacatt cggtttatgt ccatcccgat catcagggca aaggtctggg gcgtaaattg
      421 ttaagccgat tgattgatga agcgcgggat tgcgggaagc atgtcatggt cgccgggatc
      481 gaatcgcaaa atcaggcttc gctgcatctg caccagtcgc tgggatttgt cgtcaccgca
      541 caaatgccgc aggtaggcac taaatttggt cgttggctgg atctgacatt tatgcagttg
      601 caactcgacg agcgcactga accggacgcg attggatgaa tcagtcgctc acccttgctt
      661 ttttgattgc cgccggaatt ggtctggtag tccagaacac gctgatggtg cgcatcaccc
      721 agacctcctc taccattctc atcgccatgt tgctgaactc actggtgggg attgtgctct
      781 ttgtttctat tttatggttt aaacaaggca tggcggggtt tggcgaactg gtgtccagcg
      841 tgcgctggtg gacacttata cctggcttgc tgggttcgtt ttttgtcttc gccagtatca
      901 gcggatatca gaatgtggga gcggcgacaa ctattgcggt actggtcgcc agccagctga
      961 ttggcggtct ggtgctggat atcttccgca gtcatggcgt accactacgg gcgttgttcg
     1021 gcccaatatg tggcgcaatt ttacttgtgg ttggcgcctg gttggtggcc agacgctcat
     1081 tttaatcaga ggatggtgcc gcctttggtt aactgttcgt ggccgcttcc atatcttctt
     1141 tatgcttgcg accgtgatga gcaatagcgg tgcgcaaacg ctgctgctgg gtgtagcgat
     1201 cttcccgact taactccgcg tcatcgctca gggcgattaa taattcgttc atatgggcaa
     1261 tgacgctctc ttcaatcgcg gcatccacgc gcgcgatgac ttcatccaga tgcgacatac
     1321 tttctcctta gccacttgtg aaatttatcg attaatccaa atgatataag tctgcgtggt
     1381 gccgtcaagc cagtgagtaa gatttaaaac agtgaaaaca acagcgccac cgggaaactc
     1441 atcggccatg tggcccccac cagaaaggcg cttaagaaac ggatgcgttt cttatcgtga
     1501 gaaagaaacc aggtgataag aaatgcgatt gctgccataa ccgcataaaa aacaagcata
     1561 tgttgataga gagacatgta cgggcgtcca gccatatgaa taaaacgcgc gcaaatatgc
     1621 gccatttttt gacgtgcatc aagttttttc accagtttga gaaaaaatca tcaaacagca
     1681 acttgccctt atccatcttc agaatgagta ttattgcgga ttttcttaac ccgtactata
     1741 cacatagggg caatgataaa aggtggcaaa aatgaatgtt tccagtagaa ctgtagtact
     1801 gataaatgtc tttgctgctg ttggtttgtt tactcttatc tctatgagat ttggctggtt
     1861 tatttgatgt agtgattttg cacgcagcac gtactgtcag agaaatcaac tctctgacag
     1921 tacgcaagat cttctcatac tctccaagtt tgcaacatta tccttaatat ttacttcttt
     1981 ttgctggcat ctacattgaa atgagtagaa aacatccccc tactgtcgtc tatacaaaca
     2041 gcttactgat tcaaacggaa tcttgttttc acatttttct atcacgaata ctttatacgc
     2101 gcgggagtct ataaatgctt tatactttca aggaataacg taaatataaa aagatggcgt
     2161 aaagtatatg catggcagga tgtttgcaac ctccttgatg acgctgcccc attgaaattg
     2221 caaaccccgc cctggttatc ttgcccattt atcatgcaaa acagataatt ataggcctct
     2281 gaatacctca gaaagattga attttttata gtccctctct tgaatatttc catttgatat
     2341 caatcgattc atctcttcaa caaaataatt tatatcatca cacttgttat tatccggaag
     2401 atctgagtag ttataattcc accattttat tctcaacagt tcatcaataa catttgaatc
     2461 aaacctaaat ctgattattt ttgcaggaac tcctgcaact atggcatatg ggggcacatc
     2521 tttggtaaca acggaattgg cagcaataac agcaccatca cctattgcga tgccaccttt
     2581 caatacaaca tcatttccta tccagacatc atttcctata atcggatttt caggtttggt
     2641 aatgaatggt ttcaatgtcc agctacgttc aaattcactc atagcaaact tatcaaattc
     2701 accgttataa gtcatcatgt gagtagtaaa acgattcaaa ggatgatttc cacccattat
     2761 ttttacattt gatgcaatac tacaaaatcg acctatacgt atattccctg aaaaatggca
     2821 ggtcggaaat gaaaaagcgc cgacactgga gaaatttttc acaggcattg ttgaatattc
     2881 ttcagcaata acattatctt caattgttat cttgtcacct tttgagaaaa gaaataaatt
     2941 cctttcttta tacgacttct tccccttatg atttaaaaag attttattat ccatacaaaa
     3001 ctcaaagtgt tttttcgtcc atctgaaggt aagggtttta tttttatctt ccgtgaattt
     3061 gtacaattga actttcattg ttaatacttc tacttataaa ttattagttt aataacgata
     3121 ctctaaagcc ttatgctaat caactgtgac aggatgaaaa atcgccatca aacatttagc
     3181 ctaattttta cgccagttat taatgtaatc attctaatac ttaaccgcca ttaaactcag
     3241 aacctaaaac gatagtggcc gtcctataac agccactaac atctaactta atgtttaacc
     3301 atgacgtggc ggacgacggt gtaatcctcc agcccataaa gtgacatatc cttgccgtaa
     3361 ccagaaagtt tctgcccacc gtgcggcatt tcacttacca gcatgaaatg ggtattgacc
     3421 caggtacaac catattgcag ccgtgcgctg acgcgatgcg ccctgcccac atctttcgtc
     3481 cataccgaag atgcaagtcc gtactggctg tcattcgccc agttcaccac ctgttcttcg
     3541 ttgtcgaagg gcgtaacact cactactgga ccaaatacct ctttttgcag gatggcatcg
     3601 tcctgtaatg cgccagccag cagcgtcggc gcatagtaat agccattacc cttgcgcttt
     3661 tcaccgccag tgatcacttt gatgtgccct gtcgctttcg cctcttctac tgccttgctg
     3721 acgcgttcga gatgcgccag cgagcttaaa ggtccaagct ccgtagactc gtcatccggc
     3781 gcaccagatt ttaacgttgc caccgcaggc acccagtttt tccaccagcg tatcgtaaat
     3841 gcctttttgc gcgtagatcc gacaagccgc agtacaatcc tgtccagcat tgtaatagcc
     3901 aaatgtacgt acaccttcga ccactgcttc aatatccgca tcatcaaaaa caatcactgg
     3961 cgctttgcca ccaagttcca tatgagtacg cttaatggac gacgcggtat ggctgatgat
     4021 gtgctcgccg gtggcgatag agcccgtcag cgacaccatc cgcactttgg gatgaccggt
     4081 cagcggatca cccaccgttt tgcctctgcc aaacagtatg ttaatcacgc ctgccgggaa
     4141 gatatctttc gccagctctg ccaacttcaa cgcggtcagc ggggtaattt ctgatggttt
     4201 aagcactacg cagttccctg ccgccagcgc cggagcaagt ttccacgcgg ccatcatcag
     4261 cggataattc cacggtgcga tagaagccac gccccccaac ggatcgcgac ggatcatcga
     4321 agtatgacct tcaaaatatt cacctgccgc cagaccattc agacagcgcg ccgcacccgc
     4381 gaaaaagcga aaaacatcga caatcgccgg gatttcatca ttgaacgcac tatgcagcgg
     4441 tttgccacaa ttacgggatt ccagttcggc aaaaacctga ccattttctt cgataacatc
     4501 cgccagtttc agcaggcatt ccgcacgcgc tttcggtgtg gtttgccccc attcagcaaa
     4561 tgctgcatct gccgcacaca cagcagcatc gacctgctct gcggatgcct cggcaatctc
     4621 cagtaaaacg tcccccgttg ccggattata gacaggctgt ttttcccctt cgccgctaac
     4681 cagttctccg ttaatcagta acttatgttg catagattat tcctgtatca gtggttattg
     4741 accattgtcg ccttcgcggg ttagccacca ggcccccagg atcggcaagg ttgttaccaa
     4801 cataaccagc agtgccacca cgttggttac cggtacatca cgcggtcgcc caagctgatt
     4861 gagcaaccac aacggtaacg ttcgttcatg acctgccgta aaggtcgtaa cgatgatttc
     4921 atcgaacgac aaggcaaacg ccagcattcc tcctgccagt aacgccgaac tgagattcgg
     4981 caacactacg tagcggaagg tttgccagcc attggcccca agatccattg acgcctcaac
     5041 cagactccac gaggtgcggc gaaaacgggc gatgacattg ttaaacacca ccactacgca
     5101 aaaagtcgca tgaccgacca cgatggtgaa aaatcccggc tccagattga tggttttaaa
     5161 ggcggttaat agcgccagac cagtgacaat gcccggcagc gcaatgggca gcagcaataa
     5221 cagcgaaatg gcgtttttgc cgaaaaagtc tcgtctccac agcgcggcag ctgccagcgt
     5281 ccctaacacc agcgcagcgt caggccctgc ggtggaaaac taaacgccgc atcttcagtg
     5341 ttaaacgcat aggcggcgat tatcaggatg ggaaaatgta ggaaaacaac gccgccccag
     5401 gccgccagtt tgaggaaaaa cggtgcgcgt tcagagtgca tcgaacgctc ccagacgttt
     5461 cacgaacgcc aggtacagtg cgatgagaat aatcggcacc agggtgaatg ccgccgccat
     5521 cggcatattg ccaatcgccc cctgctggga ataaaccata ttgccgataa aatatcctgg
     5581 aggaccaacc agctgcggga cgataaaatc gcccagtgtg agtgagaagg taaagataga
     5641 gccagcggca atacccggga ttgccagcgg cagcaccaca tagcgaaagg tttgtcgtgg
     5701 acgtgcgccg agatcagccg acgcctgcaa caatgacggc ggcaaacgct caagcgccgc
     5761 ctgaacgggc aggat
//
LOCUS       DAJTYF010000181         5759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTYF010000181 DAJTYF010000000
VERSION     DAJTYF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.182"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(368..1873)
                     /gene="glpD"
                     /locus_tag="OO590_003890"
     CDS             complement(368..1873)
                     /gene="glpD"
                     /locus_tag="OO590_003890"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6639051.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            2063..2389
                     /gene="glpE"
                     /locus_tag="OO590_003891"
     CDS             2063..2389
                     /gene="glpE"
                     /locus_tag="OO590_003891"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6639052.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            2434..3264
                     /gene="glpG"
                     /locus_tag="OO590_003892"
     CDS             2434..3264
                     /gene="glpG"
                     /locus_tag="OO590_003892"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6639053.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            3281..4039
                     /locus_tag="OO590_003893"
     CDS             3281..4039
                     /locus_tag="OO590_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6639054.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(4021..5619)
                     /gene="rtcR"
                     /locus_tag="OO590_003894"
     CDS             complement(4021..5619)
                     /gene="rtcR"
                     /locus_tag="OO590_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6639055.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPCGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT         1299 a         1514 c         1540 g         1406 t
ORIGIN      
        1 gaggtttgca atttactctt tacgccaaca atttaccttc tgctcaatat ttttatgctg
       61 acgctgggcg cattaatgct atttttctcg gggcgagtgg ggaacgtgcc tgagttttgt
      121 tacgttgggt atgatggcgt gggctttgcg gtaattccct gaattaaaaa gtaggttaga
      181 tttttgacaa agtgcgcttt gttcatgccg gatgcgacgt gaacgtctta tctggcctac
      241 aagaccatgc aaattcaata tattgcataa accacgtagg cctgataagc gtagcgcatc
      301 aggcagtttt gtgtttgtca tcagtctcag gccagattga aatctgacct gatcacctta
      361 cgttaattta cgacgccagc gacaacttct gctgcgtata ttccaccagc cactgactca
      421 cacgagattg ttgatccgca ttcagccaca tgccttgttt tgtgcgacgc cacagggcat
      481 cgtcggtgcg gcggacccat tcgtgatcca ccaggtattt cagttccgct tcgtagaact
      541 catgaccgaa atcttccccg agatcgctta ccgttcccgc attgccgagc agcagctcgc
      601 tgttgctgcc gtaagtgcgc gcgtagtgac gtgccagcga ttcagtcagg aacgggtaac
      661 ggcggcgcag gcgagcggcg taatcgtcgc ggtcgccttc aatggcacca cccggcagca
      721 cgctctcttt cgtccacgcc gggccaatac cctgataata cggcgtcagt ttttccagcg
      781 catgttctgc cagttttcgg taggtggtca gcttaccgcc gaataccgac agcagcggtg
      841 ctttgccgtt ttcatcatgg atatcaaggg tgtaatcacg ggtaatggct tgcggcgagt
      901 cggactcatc atcacacagc ggacgcacac cggagtaggt ccagacgata tcgtcacggc
      961 ttagctgctt tttaaagtgc gcgttataca ctttcagcag gtaattgatt tcactctctt
     1021 caatcttcac tgctttcgga tcacctttgt attccacatc ggtggtgccg atgatggaaa
     1081 actcgtccat ccacgggatc acgaacacaa tgcgtttatc ttcgttttgc agaatgtagg
     1141 cctgcttttg ggtatgcacg cgcggcacca caatatggct gcctttgatc aggcgaatgc
     1201 cataaggcga aggcagatgc atcccctcgt cgaagaactg tttcacccac gggccggtgg
     1261 cgttaaccag gccgcgcgct tgccagctat attttttgcc ggtatcgata tcttccgctt
     1321 ccacaatcca caggccgttt tcgcggcgag cagaggtggc gcgagtccga gtaagcactt
     1381 cgccgccttt acgcaccacc atctgggcgt tggcgagtac cagacgggcg tcgtctaccc
     1441 aacagtcaga atattcgaat ccgcgcttaa tttccggttt taacactgaa tttgcgccaa
     1501 aacgcaaacc aattgatccc ggcaggctgg tgcgtttacc cagatgatcg tacataaaca
     1561 gaccaatgcg aatcatccac gccgggcgca gatgcggacg atgtggcagg cgaaaacgca
     1621 tcgggaaggc gatatgcgga gccattttca gcagcacttc acgttcagcc agcgcctcgc
     1681 tgaccaggcg gaattcatag tgctcaaggt agcgcaggcc accgtgaatg agttttgaac
     1741 tggcggaaga ggtcgcgcaa gcgagatcct gcgcctccag catcagcacg gataaaccgc
     1801 gtccagcggc gtctgccgcg ataccagcac cattgatgcc gccacctatc acaatcagat
     1861 ctttggtttc catgctgccc tcattcactt tcgttaaagc tcataaatgt tcgttatcga
     1921 acatattagc aaagaatcgc gctttgggta acattgaaaa aacattttag agtgatatgt
     1981 ataacattat ggcgtttatc tgccgcttcg acgtaaactg tgcggtaaat ttgcccactt
     2041 gtttgtaaag aaagagagac gcatggatca gttcgaatgt attaacgttg ccgacgcgca
     2101 ccagaagttg caggaaaaag aggcggtgct ggtcgatatt cgcgatccac agagtttcgc
     2161 tatggggcat gcggtgcagg ctttccattt aaccaacgac acgctgggcg cgtttatgcg
     2221 tgataacgac tttgacactc cggtgatggt gatgtgttat cacggcaata gcagcaaagg
     2281 ggcggcgcag tatctgctgc aacagggcta cgatgtggtc tatagcattg acggcggctt
     2341 tgaagcctgg cagcgacagt ttcccgcaga ggtggcgtac ggcgcgtaac gctttatact
     2401 gtcccctttt gtgtggaata agcgacagca acgatgttga tgattacctc ttttgctaac
     2461 ccccgcgtgg cgcaggcgtt tgttgattac atggcgacgc agggtgttat cctcacgatt
     2521 caacaacata accaaagcga tgtctggctg gcggatgagt cccaggccga gcgcgtgcgg
     2581 gcggagctgg cgcgttttct cgaaaacccg gcagatccgc gttatctggc ggccagctgg
     2641 ctggcaggcc ataccggcag tggcctgcat tatcgccgtt atcctttctt tgctgccttg
     2701 cgtgaacgcg caggtccggt gacctgggtg atgatgatcg cctgcgtggt ggtgtttatt
     2761 gccatgcaaa ttctcggcga tcaggaagtg atgttatggc tggcctggcc attcgatcca
     2821 acactgaaat ttgagttctg gcgttacttc acccacgcgt taatgcactt ctcgctgatg
     2881 catatcctct ttaacctgct ttggtggtgg tatctcggcg gcgcggtgga aaaacgcctc
     2941 ggtagcggta agctaattgt cattacgctt atcagcgccc tgttaagcgg ctatgtgcag
     3001 caaaaattca gcgggccgtg gtttggcggg ctttctggcg tggtgtatgc gctgatgggc
     3061 tacgtctggc tacgtggcga acgcgatccg caaagtggca tttacctgca acgtgggtta
     3121 attatctttg cgctgatctg gattgtcgcc ggatggtttg atttgtttgg gatgtcgatg
     3181 gcgaacggag cacacatcgc cggattagcc gtgggtttag cgatggcttt tgttgattcg
     3241 ctcaatgcgc gaaaacgaaa ataattccag ggatttataa atgaaacaaa cacaacgtca
     3301 caacggtatt atcgaactgg ttaaacagca gggttatgtc agtaccgagg aactggtgga
     3361 gcacttctcc gtcagcccgc agactattcg ccgcgacctc aacgagctgg cagagcaaaa
     3421 cctgatcctg cgccatcatg gcggcgcggc gctgccttcc agttcggtta acacgccgtg
     3481 gcacgatcgt aaagccaccc agaccgaaga aaaagagcgc atcgcccgca aagtggcgga
     3541 gcaaatcccc aatggctcga cgctgtttat cgatatcggc accacgccgg aagcggtggc
     3601 gcacgcgctg ctcaatcaca gcaatttgcg cattgtcacc aacaatctca acgttgctaa
     3661 cacgttgatg gtaaaagaag atttccgcat tattctcgcc ggtggcgaat tacgtagccg
     3721 cgatggcggg atcattggcg aagcgacgct cgattttatc tcccagttcc gccttgattt
     3781 cggcattctg gggatcagcg gcatcgacag cgacggctcg ctgctggagt tcgattacca
     3841 cgaagttcgt accaagcgcg ccattattga gaactcgcgc cacgttatgc tggtggttga
     3901 ccattcaaaa tttggccgta atgcgatggt caatatgggg agcatcagca tggttgatgc
     3961 cgtctacacc gacgccccgc cgccagtaag cgtgatgcag gtgctgacgg accaccatat
     4021 tcaactggag ctgtgctgat cctgcacggc ctcccacgtc agaccaaaac gcgccaggta
     4081 tttgcgtagc cgatccgcgt cattgacgct ggctttgccc tggcgcgaaa catcaaaaag
     4141 ctgtcgtccg gcagcagaaa gcgactttgc ctggcggcag atagcgataa cgtgttccag
     4201 ttgcatacgg tcgaagagat cgatgttttc tgcctctgcg cccagcaacg ccgtaagcgc
     4261 ggaggggcgg ctctcctgcc agttatagcg cagacggttt atctcatctt ccaccacgtc
     4321 cagagtgatg cgtccgctag tggcaaaggt ggccatccgc gtgacgctgg cagaaagttc
     4381 gcgaaagtta ccgcgccatg tagcctgggg agatgttgca aaggccagcc aggcacgccg
     4441 cgcttcggtg ttaaaacgca cgctgtcacc agtgagtgtg gcgtggcgct ccacttcata
     4501 atccaggttc ggttcaatat cttcctggcg ctggcgtaga cccggcaggg tgaaggtcca
     4561 gagattgatc cgcgcgtaca gatcttcgcg gaatttgcct tcggcaacca gctggcgcaa
     4621 atcgcgcacc gttccggcga taagctgaaa atcactgctc acctggcgat cgctgccaaa
     4681 cgggtaaaag gttttctctt caatagcttt cagcagcatt gcctgttcgt ctgcgcccag
     4741 ttcgccaatc tcatcaagaa acaacattcc gccgttggcg ctgcgtaata atccttcacg
     4801 agattcccgc gccccggtaa acgcgccttt cacatgacca aacagcgtcg acatcgcggt
     4861 atcgccgcgc agggtggcgc agttcacttc cacaaacgcg ccgctaaact gatgccgtgc
     4921 ctgttttaac tcgaagatgc gtcgcgccag aaatgactta cctgcgccgg tcgggccgtt
     4981 aagcagaatc ggcgcgcggg atttaatcgc cactttttcg atctgctcaa tcatgcggtt
     5041 gaagtggggg ttacgcgtgg caataccgga tttgaggaaa tcgagcgttt gctgccgttc
     5101 ctcggcaaag cggctggcga tggcgttata acggcttaaa tcgagatcga taatcgtcac
     5161 ttcacctggg ccgcacggct gttctttttt gcgcggagtt gaagattgta tcaaccgggc
     5221 gggtagataa cgcgcttccg ccagcagaaa ccagcaaatc tgcgcgacgt gggtgccggt
     5281 ggtgatgtga attaaatagt cttctttttc tggctgaaac tcgtaaccac gggcgaaatc
     5341 atgcaggcag gcgtaaacct cttcgaaatc ccacgggtta tgcagttcaa tctcaacgcc
     5401 caccacttct gtttctggcg aaacgctggc gatatcgcgt ttaagcgttt caaatagcga
     5461 gcgcgagcgg gcatcgtgca ataattccag tcgatcgatg accaacgatt cttgctggca
     5521 tatacagagt gacggacgcc attttgacca gcgctgactg ccgcgtccgg cataatccag
     5581 cacggtaccg acaaagccaa aagccactgt tttacgcatc ttagatatcc ttataaaagg
     5641 cgatatttca ttatcggtaa atcggctttg cgggaaatct ccgtttacgg acataaagat
     5701 ataatctttt tagaaacaat aaattaaaaa attaatctgc ttatttaatt ttctggcac
//
LOCUS       DAJTYF010000182         5759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTYF010000182 DAJTYF010000000
VERSION     DAJTYF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.183"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..1110
                     /locus_tag="OO590_003895"
     CDS             <1..1110
                     /locus_tag="OO590_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR6639056.1"
                     /translation="WPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMA
                     VAIQHGSFIEDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTDQ
                     ALNAEIQKGEEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRD
                     PFDWAEKNLQEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVF
                     IDNYAAIPSESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFID
                     ARGQRPLKVKDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHD
                     QPFVQGLTACAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(1122..1934)
                     /locus_tag="OO590_003896"
     CDS             complement(1122..1934)
                     /locus_tag="OO590_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639057.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(1938..2723)
                     /locus_tag="OO590_003897"
                     /pseudo
     CDS             complement(1938..2723)
                     /locus_tag="OO590_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
     gene            complement(2720..3388)
                     /locus_tag="OO590_003898"
     CDS             complement(2720..3388)
                     /locus_tag="OO590_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6639058.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(3452..4090)
                     /locus_tag="OO590_003899"
                     /pseudo
     CDS             complement(3452..4090)
                     /locus_tag="OO590_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
     gene            complement(4103..>5759)
                     /locus_tag="OO590_003900"
     CDS             complement(4103..>5759)
                     /locus_tag="OO590_003900"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR6639059.1"
                     /translation="ICRLTSRETCVAAIGQAGENLIPLSGMLNSRNHSGGAGTGAIMG
                     SKNLKAIAVEGTKGVNIADRQEMKRLNDYMMTELIGANNNHVVPSTPQSWAEYSDPKS
                     RWTARKGLFWGAAEGGPIETGEIPPGNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHS
                     CPIRCMTQMNIPRVKEFGVPSTGGNTCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVG
                     LNLFDDYGLWCNYGQLHRDFTYCYSKGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFY
                     YRLAHRVGELSHLAYGSYAIAERWNLGKEYWGYAKNKLWSPFGYPVHHANEASAQVGF
                     IVNCMFNRDCMTHTHINFIGSGLPLKLQREVAKELFGSEDAYDETKNYTPINDAKIKY
                     AKWSLLRVCLHNAVTLCNWVWPMTVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLD
                     LAAERIFTLHRAYTVKPMQTKDMRNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHA
                     SLTMFYKEMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         1468 a         1542 c         1335 g         1414 t
ORIGIN      
        1 tggcctgatg aagaagaagc aaccagaacg tattttccca gcccgtggtc aggcctgatg
       61 gaagcaaagg tcgatgcgtg taacgtgggt attatgggaa catccttgag cggactggat
      121 gcggcaatgg cagtggctat tcagcatggt tcgttcattg aagatgataa acaacacgtc
      181 gtttttcacc gcgataacgc aagtgaaaag ctaaatatta cgttgatgtc gcgcacgggt
      241 attttacccg aagccgattt ctattgccct attccctgcg agcccttaca catcgtcacc
      301 gatcaggcat taaatgctga gattcaaaaa ggtgaagagg gccttttgga tcgggtattt
      361 agattgatag tagaggaaat caagtttgct gatccagact ggagccaacg catagcctta
      421 gagagcctga atgtcgattc ctttgctcaa gcctggtttg ccgagcgcaa acaacgcgac
      481 ccatttgact gggcagaaaa aaatctccag gaagtcgaac gcaataaacg agaaaaacat
      541 actgttccct ggcgttatgt cattctgcgc ctgcatgaag ccgtacagga aattgttcct
      601 catctgaatg aacacgacca taaacggttc agtaaaggcc ttgccagggt tttcatcgat
      661 aattatgcgg caatcccttc agagtctatt cgtcgactac tggccttacg tgaagcgggg
      721 atcattcata ttctcgccct cggtgaagac tacgaaatgg aaattaatga gtcgcgcacc
      781 gtcctgaaaa cggaagacaa cagctactcg tttgacgttt ttattgatgc ccgcggacag
      841 cgtccgctta aagtgaaaga tattcctttc cctgggctac gcgagcaatt acagaaaaca
      901 ggggatgaaa tccctgatgt tggcgaagat tatacgttac agcaacccga agatattcgt
      961 ggacgcgtag cgttcggcgc gttgccctgg ctgatgcacg accagccttt cgttcaggga
     1021 cttacggcat gtgcagaaat tggtgaggcg atggctcggg cggtcgtaaa gcctgcatcc
     1081 cgtgctcgtc ggcgtctttc gtttgattaa aggtaaagct atcagcactc aacaaactga
     1141 tgctgttcat taagacgata gcggactccg gttcgaatat tgttttcacc ttcaatggcg
     1201 acggcgaagc gaactctttc gccgtcatga tatgccagtg cggcgcttcc ccctagcccg
     1261 agaataattg agtcgaccac gcccgtgctc actaccgttg aattttcacc gctggcaatg
     1321 atgtgtacat tgtcgccgct gttggcaatt ctggcgtttg cgccaaagct ggcgatttgc
     1381 accaggtcac cattactggc gacatgacac cgttcaccca atgtacaaac gcgtactcgc
     1441 attccggtat tcgcaatccg actactatct ccagcgctgc ttatccgcga agaattaccg
     1501 gttacagcaa cccgtgcgcg ttcaccggag ctgccaatgt gactgttaaa gccgacactt
     1561 ccaatccgca cgctataacc catactggca atttgcgcgg catacccagc ggttgcgatt
     1621 cgggcgttat cctcacaaca gccgatgcga gcatgatcgt cgctgcgggc aaactgactg
     1681 caaaatggta tctctccggg gtctgtggcc agtttttgca tagaggaaac ttcctgatgc
     1741 gcgaagtttt catccgccag ccattttttc cacgcatact caaccagact ctccgcccag
     1801 tccgtatatc cttcctcaat cagtgcatga cgaatatcgg cataactacc gccgcgtggg
     1861 aaatggcgca ggaaccagcg atacatcacc gctccgatac gccattctcg caggtctgct
     1921 cgtgtaatga tcattcgtta gtgccggtga tagccatcga ccatgctttt aaaggtttcg
     1981 tgtggcgtac gccccgtggt gcaaagataa agatgaccga agataaaaaa gaggcttata
     2041 aatgccagag caaaatgcgc ctgcaataac cagtatctta cgccaggaaa cacatctccc
     2101 acggcttgcg gatagagaca caacagcccc gtcaatagta acaacggcag caatccatac
     2161 atgacaccaa cataggcgac ctgctgtaag ggattaaatt tagactgtgt tgttgccggg
     2221 aaaggatgtt cttccccctg cataatgcca aacaaataaa atcgcgtttg ttttgccgct
     2281 cgttccagcc acccctgacg acgaatgcga tagtggtgac cattatcccc aacggcattg
     2341 atcagcacaa agccgagcca gcatgccagt aacaaaaatc agcaaacttc atgcaccgca
     2401 accagacttt taaccgcagt tgcgcccacc atcgcaaagt gatttatcag cccacttgcc
     2461 agcaacaata caaagagtaa cgcattcgac cagtgccata atctgaccgc tttgctgtaa
     2521 agataaacct tctcaccatg atcggtggct gatttcttca cgccacgagc acgaagcaag
     2581 gcgtgcagcc ccagcaccaa ccacatccca accagcaata ctccggcaat gatcaaccac
     2641 accggccaaa attcgggagt gaactgtggc acatagttcg ctaactggct ctgaaactgt
     2701 tcagcatgtt gcgacgggtt catacatttt cctttttaat caaatgttga ccgaaccggc
     2761 gatacagatg cggttttccg gcaccaggta gctgatattg atagtattta ttatcctgca
     2821 accacgcctg aatttctggg ctatcttcgc gaccaaagat caatgcgtgt tccggacagg
     2881 cgctgacgca aatgggcgga aagccttttg ccagtcggga ctcagcgcag aaatcacatt
     2941 tatctgccac tttagtgaca gggttaaggt agcgaacctg gtacggacaa gcgccaatgc
     3001 aataactgca gccgatgcac tgcgattttt ctacccgcac gatcccctgc tcatcacgcc
     3061 acgatgcccc cgtcgggcat acgtcgatgc acggagcatc ttcgcagtgc tgacatgact
     3121 ggcgaaaaaa gtgatactgc gtttcgttgt cgttatcggt gactggaatg tgtgctatcg
     3181 ataaacggct tccctgagcg gggacgtggt tagttttacg gcatgcgcgg gcgcaaatat
     3241 tacagccatt gcaccgtgac tcgtcatgaa tcatggcata ccgaacttcc ttttcttgcc
     3301 tcgtgttcgc taacagagaa gacgcagaac cagtaaaaaa gattactgtt cccatcccca
     3361 gaacaaattt gcgtcgagtg aaggacatct gattatcccc taatgcagtg gctgcaactg
     3421 tccaccctat ccatgacatc tccctgaaat ttcaggggtt ctgaaactgc tctacctcga
     3481 accattcgca taagccataa gccttgccat aggtcataaa tcgctcgcta aacaagtaca
     3541 acaccgccgt cgatgactcc acctgccgta tatcggcata ttcaggtgca acatccatcg
     3601 cggcggtcgc ggcttcaatc tgcgcttcct agaagtaata tggcgcttgc atcagcatgg
     3661 cgactttgta aggacgagga tacgtctggc actcaaaacg aacggcgtgt gcaatggcgc
     3721 gacaaatgtc ctgctccaca acttgcaaag acatcgccgc gtagttttca ctcatggcct
     3781 gcgttgagta atagtaatca tcctgacttc ccgtcataca agcgatatcc gcgtagtctc
     3841 cgttctgctt aatgctttcc agcaaaactg acagttcctc ttctgcaacc gaatagggtg
     3901 gcgagagaaa cacggtgcgc gccaccagct gcccagccgc agaatgctgg cgaatgaact
     3961 cagccaggat ttcagcaggt tctggttcgg ttaccgtatc aacggggtga cgcatcccct
     4021 gcaaccattg ccgcttcgcc tcgtcaactt cgctgacttt cgctaacggc aattcatcct
     4081 gatggttcat cttacccatt ccttacgcag gcagtagatt gtgtgctgcc agatcggcgg
     4141 caatatcttc cagccccaga cgctgcaatg tttcgcgggt tggacaacca agctgtgggt
     4201 cccagcccat ttctttgtag aacatcgtga gcgaagcatg catatcgtca cgatccattt
     4261 tgtcagtacc ttcagtaaag accgggatct gcggatcctt gtcgaaaacc caggaacaga
     4321 taagatcgtg ttcattacgc atatctttgg tttgcatcgg ttttaccgtg taggcacgat
     4381 gcaacgtaaa gatacgctct gcagctaaat ctaatttttc ctgagtcatc tcttcgccgg
     4441 tgatcgcttt gaagaatttg gcttcaagcg ccagatcgcc ccggtaatta cggcttttca
     4501 gcggcgaaac ggtcattggc cagacccagt tgcacagggt gacggcgtta tgcaaacaga
     4561 cccgcaacag cgaccacttt gcatatttaa tttttgcgtc attgattggc gtgtagtttt
     4621 tggtttcatc gtaagcatct tcagaaccaa aaagttcttt cgccacttca cgttgcagtt
     4681 tcaatggcaa gccggaacca ataaagttga tatgggtatg cgtcatgcag tcacggttga
     4741 acatacagtt aacaatgaaa ccgacctgcg ctgacgcttc attggcatgg tgaaccggat
     4801 agccaaacgg cgaccagagt ttatttttcg cgtagcccca gtactcttta cccaaattcc
     4861 agcgttctgc gatggcatat gaaccataag ccaggtgact cagctcaccc acacgatgcg
     4921 ccagacggta gtaaaaatct ttaatgaagt taacgtcacc cgcttccagt tgatcccagc
     4981 gaatttctgc atactcttca gctggcagaa cacgcttgaa cacacctttg ctgtaacaat
     5041 aagtaaagtc gcgatgcaac tgcccgtagt tacaccatag gccgtagtca tcgaacaaat
     5101 tcagaccaac caggttacca atcacacgac catcgtcttt atcttcaaaa tctttcggcc
     5161 cgttcgggaa gatggtggta tggacaaagt ttgctacaca agtgttacca cctgtgctgg
     5221 gcacgccaaa ctctttcacc cgaggaatat tcatttgggt catacaacgg atcgggcaag
     5281 agtggcagcc gctcattttt actgtgtatt tctctgccgc cggtcctaag tcaaaaacgg
     5341 atttataggt acgaaagccg accgtatttt gattgcctgg cggaatttca cccgtttcaa
     5401 tcggaccacc ttcagccgcg ccccaaaaca gccctttacg tgctgtccag cgtgacttgg
     5461 gatctgaata ctctgcccac gattgtggcg tacttggcac gacatggttg ttattcgcac
     5521 caataagttc agtcatcatg taatcattga gacgcttcat ctcctgacga tcggcaatgt
     5581 tgaccccttt cgtcccttca accgcaatcg ctttcaggtt tttcgaaccc attattgcgc
     5641 cagttcccgc accgccgctg tggttacggc tattcaacat gccagagaga ggaataaggt
     5701 tttccccagc ctgaccaata gccgccacac aggtttcccg actggtcaat cgacaaatt
//
LOCUS       DAJTYF010000183         5698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTYF010000183 DAJTYF010000000
VERSION     DAJTYF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.184"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..109)
                     /locus_tag="OO590_003901"
     CDS             complement(<1..109)
                     /locus_tag="OO590_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HCR6639060.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIG"
     gene            complement(366..647)
                     /locus_tag="OO590_003902"
     CDS             complement(366..647)
                     /locus_tag="OO590_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6639061.1"
                     /translation="MWGVELLAIRYAAWIKPEFEIEVYEVFKTVVRLGVGAMSRLNRI
                     DHIINTETKAISQCASQMAKWGVGGRKRLLHVARERAANEVQMYLPGMV"
     gene            complement(671..871)
                     /locus_tag="OO590_003903"
     CDS             complement(671..871)
                     /locus_tag="OO590_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6639062.1"
                     /translation="MKYPTVIVNGVSVRVDEDGRYNLNDLHAAAVANGEATEQQRPSQ
                     FLRSAQIKRFIKALWNKFNHLK"
     gene            complement(1077..1229)
                     /locus_tag="OO590_003904"
     CDS             complement(1077..1229)
                     /locus_tag="OO590_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639063.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSKSRLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(1217..1684)
                     /locus_tag="OO590_003905"
     CDS             complement(1217..1684)
                     /locus_tag="OO590_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6639064.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(1681..2157)
                     /locus_tag="OO590_003906"
     CDS             complement(1681..2157)
                     /locus_tag="OO590_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR6639065.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
     gene            complement(2141..2464)
                     /locus_tag="OO590_003907"
     CDS             complement(2141..2464)
                     /locus_tag="OO590_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR6639066.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            2689..2907
                     /locus_tag="OO590_003908"
     CDS             2689..2907
                     /locus_tag="OO590_003908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639067.1"
                     /translation="MATAQFGLWRPVLISGLLQLPTAGYVATPNPAKDSCPYTSACAF
                     TTTERALANQARRLFTIIGSMLLPVVQI"
     gene            complement(3176..3799)
                     /locus_tag="OO590_003909"
     CDS             complement(3176..3799)
                     /locus_tag="OO590_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6639068.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTELWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(3796..3984)
                     /locus_tag="OO590_003910"
     CDS             complement(3796..3984)
                     /locus_tag="OO590_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR6639069.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(3981..4343)
                     /locus_tag="OO590_003911"
     CDS             complement(3981..4343)
                     /locus_tag="OO590_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6639070.1"
                     /translation="MSEYQFVLPYPPSVNTYWRRRGNQYYISDKGQKYRKEVQQIILQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(4340..4630)
                     /locus_tag="OO590_003912"
     CDS             complement(4340..4630)
                     /locus_tag="OO590_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6639071.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            complement(4630..5352)
                     /locus_tag="OO590_003913"
     CDS             complement(4630..5352)
                     /locus_tag="OO590_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCR6639072.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEDCGRINGLGLNQSFCVYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(5345..5554)
                     /locus_tag="OO590_003914"
     CDS             complement(5345..5554)
                     /locus_tag="OO590_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCR6639073.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDE"
BASE COUNT         1288 a         1467 c         1299 g         1644 t
ORIGIN      
        1 tgccaatgaa ctgaaatgct tcttctgcgg ctgcgttctg gttcataacc agtttgtaaa
       61 tctctaactg gaatttctgt tcttcagtca tgggaataat ctctgccatt gttggctccg
      121 tttatccgtt aaaagggata tcagttaagt tatcccgtgt agggtataag ccattatcga
      181 gaccactcat tgaatggcct ctgcaataac cgatgtcttt ccatcagtcc gccaccacaa
      241 agaatctttt ttgccataag gctggaggtt catctttcag tggctgccag tgttatttcc
      301 ccacttactg gcttgggttg tttcgcggta ctgccgtaat gcaaaaactg gattaacctg
      361 cgaaatcaca ccattccggg caaatacatt tgcacttcat ttgccgctct ctcacgtgca
      421 acatgaagca atctttttcg cccaccaacg ccccacttag ccatttggct tgcgcactgg
      481 cttatcgctt tggtttcagt attgatgatg tgatcgattc tattcagacg ggacattgcg
      541 ccaacgccga gacggacaac cgttttgaaa acttcataaa cttcgatttc aaattccggc
      601 ttaatccatg ctgcatatct gattgccaga agttcaacac cccacacacc tggttctgca
      661 ccacctttga ttattttaag tggttgaatt tgttccaaag tgcttttatg aagcgtttta
      721 tctgcgcgct acgcaaaaac tggcttgggc gctgttgctc tgtagcctct ccatttgcaa
      781 ctgctgctgc atggagatcg tttaagttgt agcgtccatc ctcatcaaca cgaacggaca
      841 caccattgac aataactgtt gggtacttca tcagtgatta ccttttagtg atgaaccttg
      901 tcacacagga ttccggccca cagaaaggca ccgattacca aaccggcatc ctcaagggtc
      961 atcctgaaag gctctgtgtt cataagtcgc gcgtgtgaag cgcgtttgtt gcagatataa
     1021 aaaagccccg cgaatgcgag gctaaatcct ggtgtttgtg atgactggct cttatctcaa
     1081 cgcagcccct taccgcgcgc cagatgctca atatcaagca tcagcaatga gatgtttaat
     1141 ctggatttac tccagaagtg atcacccccc tgtctacaga gccagatgtg aaggatgatg
     1201 agtaaaatta tcgctatcat cgaaggcatt gcgtcctgat gtattcctga agcgttctca
     1261 gtgctgtttg gtcgcggata attccgtccc ggataccgag aacgtttcgt ccagcaactg
     1321 gagagagttc gacggtggca tcattgccca tgccggaggc gctggaggtt tcggctgagg
     1381 atggcacagg gcattttcct ttgacgagca ccctgccacc attatcaagc ttgcgccgaa
     1441 gagcatcatt ttcagctttc gcatcagcta actccttcgt gtatttagca tcgagtgcat
     1501 cagcagcacg ctggcgttgc tgcatgtcag taatggtggc ggtcgcctgc ttcagctcac
     1561 tgactttttt atctcgctgc tctttgtagg tcatggcgtt atcacggtaa tgattaaccg
     1621 cccatgacag gcagacgatg atgcagataa ccagagcgga gataatcgcg gtgactctgc
     1681 tcatacatca atctctctga ccgttccgcc tgcttctttg aattttgcaa tcaggctgtc
     1741 agccttatgc tcgaactgac cataaccagc gcccggaagt gaagcccaga tattgctgca
     1801 acggtcaatt gcctgacgaa tatcaccgcg atcaatcatc ggtaaagcgc cacgttcttt
     1861 aatctgttgc agtgccacag cgtcctggct tttcggagag aagtctttca ggccaagctg
     1921 cttacggtaa gcatcccacc aacgggaaag aagctgataa cgtccggcgg ctgttgattt
     1981 tagttttggg tttagtgtga caagtttgcg aggatgatcg gagtaatcag taaatagctc
     2041 tccgcctaca atgacgtcat aaccatgatt tctggttttc tgacgtccgt tatcagttcc
     2101 ctctgaccac gccagcatat cgaggaacgc cttacgttga ttattgattt ccaccatctt
     2161 ctactccggc ttttttagca gcgaagcgtt tgataagcga accaatcgag tcagtaccga
     2221 tgtagccgat gaacacgctc gttatataag cgagattgct acttagtccg gcgaagtcga
     2281 gaaggtcacg aatgaaccag gcgatgatgg cgcacatcgt tgcgtcgatt actgtttttg
     2341 taaacgcacc gccattatat ctgccgcgaa ggtacgccat tgcaaacgca aggattgccc
     2401 cgatgccttg ttcctttgcc gcgagaatgg cggctaacag gtcatgtttt tctggcatct
     2461 tcatgtctta cccccaataa ggggatttgc tctatttaat taggaataag gtcgattact
     2521 gatagaacaa atccaggcta ctgtgtttag taatcagatt tgttcgtgac cgatatgcac
     2581 gggcaaaacg gcattaggtt gttagcgcaa cctcatgccg cccactttca cgaagatcat
     2641 gtgtagaagg ccgcagcgta actatcactg atgaattcag gatagtcagt ggctacggct
     2701 cagtttggat tgtggcgacc ggtgctgatc tccggtttgc tgcaactgcc tacagctggc
     2761 tacgtggcca caccgaatcc agcgaaagat tcttgccctt acacatcagc ctgtgcattc
     2821 accacaacgg aaagagcact ggctaaccag gctcgccgac tcttcacgat tatcggctca
     2881 atgctcttac ctgttgtgca gatataaaaa atcccgaaac cgttatgcag gctctaacta
     2941 ttacctgcga actgtttcgg gattgcattt tgcagacctc tcagcctgcg atggttggag
     3001 ttccagacga tacgtcgaag tgaccaacta ggcggaatcg gtagtaagcg ccgcctcttt
     3061 tcatctcact accacaacga gcgaattaac ccatcgttgg gtcaaattta cccaacttta
     3121 ttcaataagt caatatcatg ccgttaatat gttgccatcc gtggcaatca tgctgctaac
     3181 gtgtgaccgc attcaaaata ttgtctgcga ttgactcttc cttgtggcat tgcaccacca
     3241 gagcgtcata cagcggctta acagtgcgtg accatgtggg ttgggtaagg ttggggatta
     3301 gcatcgttac agcgcgatat gcggcgcttg ctggcatcct tgaatagccg acgcctttgc
     3361 atcttccaca ctctttctca acaactctcc cccacagctc tgttttggca atatcaaccg
     3421 cacggcctgt accgtggcaa tctctgcatc ttgcgcccgg cgtcgcggca ctacggcaat
     3481 aatccgcata agcgaatgtt gcgagcactt gcagtacctt tgccttagta tttccttcaa
     3541 gctttgcaac gccacggtat ttccccgata ccttgtgtgc aaattgcatc agatagttga
     3601 tagccttttg tttgtcgttc tggctgagtt cgtgcttacc acagaatgca gccattccga
     3661 atccggcttg tgattgcgcc atccccatag cagccatcac atcagtaccg gaaagagagt
     3721 cagaagccgt ggcccgtggt gagtcgctca tcatcgggct ttttggcgaa tgaaatttag
     3781 ctacgctttc gagtctcatc gtcttcccct cttgccctgt ttgaccatca ggacgccgtt
     3841 aactattacg tgacgctcgc ctttgctgtc tcggttgtac ttgagcactg ttcctcttgc
     3901 gcaggaaagc atcctcgcca cttcggtctg attgcctcgt gtctggataa gaagctctgg
     3961 tatcgtttga attgtggcgt tcatacgttc tccagttcgg tgatttttat tccaagcctt
     4021 ccgcctggta ctttcacacc acgaattacg cgaatgtcat cgaattgctc gtcgtcttcc
     4081 gcaaatccgg cgtggataag ggagtcgagt aaacctttca ggatgttatc gaggtcgcgg
     4141 cggcgggagt ctggaacgtc tgcgatgact ttgatgcgga gtcgtgattt ggtgaaaatg
     4201 tctaacttga gttggaggat tatttgctga acttcttttc ggtatttctg gcctttatcg
     4261 ctgatgtagt attggtttcc ccgtcttcgc cagtaggtgt tcaccgacgg cgggtatgga
     4321 agcacaaact gatattcgct catgacttaa tcttcccctc cttcagcagt atcgcctgcg
     4381 tcctgatcac gccttcgagg tggtaaagtc tggcgtcttt gttgtcgaga ttatgggtgc
     4441 gtcggtcgat ttcatcgtga cacgcgctac aagcccatgc gccgatcagg tcgtcaggct
     4501 tcattcccgt tccgcaaatt ccagccatcc ggtaatgtgc cagaactgta gtttcaggat
     4561 taccattgca tacgccgtaa atacgtacct ggcattctct gccgcgtgct tctttgcgta
     4621 gattagccat taagcagcct cccctgttac tttcagcatt ccattatcga gcagctttct
     4681 ggtcagccac tgttgaccac gcccggtgat ttttgtggtg aacgatatct gtattccgtg
     4741 atttgtgttg accgctgttt ctttcactgt gaaatagccg cgatccatat attcctgcat
     4801 tggcacattg cgccgggaac ctgaagcaat aaggattttg tgatcgcgca tccacgcaaa
     4861 cagtttgttt ggaccaattc caacaacctt tgcaaagttt ccaatcaaaa ttccgctggc
     4921 ctcgccaacg cgatcggcaa actcaacttt aggtgcggca attgcgagct ggttttccag
     4981 ttgcattttc tgctcagcaa gatcagcagc aaggcgcaac gcttccggta gcgttttggg
     5041 gatattaacc gcagcttctt caagctctcg ccagcggtca acaaggcgag cggtgaattc
     5101 cggcgacaac tgggctacaa cgacaatact gtctcgcttt ccttgttcgc cttcgaacac
     5161 atacacacaa aaactttgat ttaagcctaa cccattgatt cttccacaat cctcaatttg
     5221 aggaagccgg ataacaccat ttttagccag cgtttcgatg gtacgtttca cattgtcatg
     5281 acgcttacca accaactcag cgatttcaat gcttgtcatt ttgatggcat tgccatttat
     5341 taactcattc atcgtcttct tcctcgtaca ttgagctatt cggatcgctc atcagttctg
     5401 cgcagcagtg ctcacacacg tgaacttcca gcacatgcag cttctgaccg caattagcgc
     5461 acgttaaagc tcgctcgacg ctttctttct ggtattgaag ggattgggat gggctaagca
     5521 tggctttcac cattaaaaag tcgcttgtaa gcatcgatgt ctcgttttgc ttcaccgagc
     5581 tttcgtctta attccatgtt ttctgattca agcttttcca tgtcttgttg gtatcgatcg
     5641 cggtgttctt tccatgcttt tcgatacgcc ttcatgtatg tcgtattggc ctttctct
//
LOCUS       DAJTYF010000184         5641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTYF010000184 DAJTYF010000000
VERSION     DAJTYF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.186"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            2..1458
                     /locus_tag="OO590_003915"
                     /pseudo
     CDS             2..1458
                     /locus_tag="OO590_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(1494..1697)
                     /gene="ydfZ"
                     /locus_tag="OO590_003916"
     CDS             complement(1494..1697)
                     /gene="ydfZ"
                     /locus_tag="OO590_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6639074.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1875..2561)
                     /gene="ydfH"
                     /locus_tag="OO590_003917"
     CDS             complement(1875..2561)
                     /gene="ydfH"
                     /locus_tag="OO590_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6639075.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSNWFSEE"
     gene            complement(2650..3396)
                     /gene="ydfG"
                     /locus_tag="OO590_003918"
     CDS             complement(2650..3396)
                     /gene="ydfG"
                     /locus_tag="OO590_003918"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6639076.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3533..5578
                     /gene="dcp"
                     /locus_tag="OO590_003919"
     CDS             3533..5578
                     /gene="dcp"
                     /locus_tag="OO590_003919"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6639077.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1400 a         1351 c         1467 g         1423 t
ORIGIN      
        1 tttgttatca gcaaaagcga cactccctgt ttatgatcgt aataacctgg ccccaagaat
       61 tgttcattta ggctttggtg catttcaccg tgcgcatcag ggcgtgtatg ccgatattct
      121 tgctacagaa catttcagtg actggggata ttatgaggtc aacttaatcg gcggcgaaca
      181 gcaaattgcc gatttacaac agcaagataa tctttatacc gttgcggaaa tgtcggccga
      241 tgcgtggacg gctcgcgtcg ttggcgtcgt taaaaaagcc ttgcacgtac agatggatgg
      301 cttagaaacc gtgttggctg cgatgtgtga accgcaaatc gcgattgtct ctctgacaat
      361 caccgaaaaa gggtatttcc actctccggc gaccggacag ttaatgctcg atcacccgat
      421 ggtcgctgcc gacgtgcaaa atccccacca gccgaaaaca gcaacatggg tgattgttga
      481 ggcgctggct cgccgtaaag cggcaggact tcccgcattc ccgcatttac cgtcatgtca
      541 tgtgacaaca tgccagaaaa cggtcatgtt atgcgtgacg ttgtcacttc ctacgcgcaa
      601 gccgttgatg taaaactggc acaatggatc gaagataacg tgactttccc atcaacaatg
      661 gtggaccgta ttgtgcccgc agtggcagag gatacgctgg cgaaaatcga acaacttacc
      721 ggtgtgcgcg atcctgctgg cgttgcctgt gaacctttcc gccagtgggt tatcgaagat
      781 aactttgtag ccggacgtcc ggaatgggaa aaagcgggag ccgaactggt tagtgatgtg
      841 ctgccttatg aagagatgaa gttgcgcatg ctcaacggca gtcattcatt cctggcatat
      901 ctgggttatc ttgctggata tcagcacatt aatgactgta tggaagatga acattatcgt
      961 catgcggcgt atggcatgat gttgcaggaa caagcgccga cgctgaaagt gcagggcgtt
     1021 gatttgcaag attacgctaa ccgattaatt gcacgctata gcaacccggc gttacgtcat
     1081 cgaacctggc agattgcgat ggatggcagc cagaaattgc cacagcggat gttggattct
     1141 gttcgctggc atctggcgca tgacagcaag ttcgatctgc tggcgctggg cgtcgcgggt
     1201 tggatgcgtt atgtcggtgg tgttgatgaa cagggaaatc cgatagaaat cagtgaccca
     1261 ctgttacctg ttattcagaa ggctgtacaa agtagtgccg aagggaaagc gcgcgtccag
     1321 tcattgctgg cgattaaggc aatctttggt gatgatttgc cagacaatag cttgtttact
     1381 gcaaaagtga cggaagcgta cttgtcttta ttagcgcatg gcgcgaaagc gaccgtggcg
     1441 aaatattccg tgaagtaaaa aaagcaatgc cgccatcggc ggcattcaca cacttagttc
     1501 atgccgagac gaatcaggtc cagaggtgcc agtttctctt cacaaccttc aacaactacg
     1561 gtttttccgc ggcggatttg ctcagcggtc agaccttcag tatcaatcga caggatcttg
     1621 ccagtgtgac cggtgccgct aaccataaca cggctgccag tggtgattgc gttacggtta
     1681 cgatcgtagg tcgtcatagg tgttttctcc tttctgattt acagtcgaag cccacgtaac
     1741 gggcgcaata taaatactcc tgtcgagtga aatatttttt gtttttgatc aagctcacat
     1801 cttttttgat gcatgcatga gccgtaaggc ggggggaaaa gcctctcatc ccggatggga
     1861 tgagagtaaa tgaattactc ttcgctaaac cagttgctgt tttcctggcg gatctggcgc
     1921 acggattcgc tgatttcctg caaatgttgc gtcattgcac gttctaccgc atcgccatca
     1981 cgtttttgca gggcagagaa aatatcaaga tgctggcgta acagcatttc tggtggagaa
     2041 acatggtcga aactcatata gcgcacgcga tcaacggtcg ctttcaggtt ctcaatggta
     2101 tcccacgcca gttgacagtc ggcaatctgc gtcaggagtt gatggaagtt gtcatcaagt
     2161 tcaaaaaaat catccagttg cttgcgctca atggcaatgc gttgctggtg aagattttgt
     2221 tccagttggt agcactggct ttcggtaatc atgctcgccg cccgacgcgc caccgcgcac
     2281 tcaatggcct gacggataaa actgccgttg cgcacctggg ccatggaaat tttgttgacg
     2341 tagctgccac gctgcggacg aatttgaatc aggccgtttt ccgccagttt aataaaggct
     2401 tcacgaaccg gctggcgtga cacattgaaa cgaacagaaa cttctttttc cgacaacggt
     2461 gtgcctggtg caatcaggca atggacaatg tcgcgacgaa gaatacgata aatctgctga
     2521 ttgacaggct gtgtgggatt aagttgcgtt tcgacggtca ttcgttcttg cttgtgagtg
     2581 agttaactgc atgagtctac cacttttttg tgacaagcaa taacccggca gttacgccgg
     2641 gtataaaaat tactgacggt ggacattcag tccggcatag ctttgggtaa ccggcatcat
     2701 ttccagggta ttgatattga cgtgagcagg cagcgttgac acccaccaga cggcttcgct
     2761 gacatcttct ggcgtcaatg caacggtatt ttgataggtt ttttccgctt taccgtcatc
     2821 gcctttaaag cggacattgg aaaactcggt gccacccacc agacccggtt cgatgtcggt
     2881 gacgcgcacc gccgtaccat gcagatccgt acgcagattc aggctaaact gacgaacaaa
     2941 cgctttcgtc gcaccgtaaa cgttaccacc ggcatacggc cagctgcctg ccgttgagcc
     3001 aatgttaata atatgaccat gattacgttc aaccataccc ggtaagacgg cgcgcgtcat
     3061 atatactagg cctttgttgt tggtatcaat catcgtttcc cagtcttcaa cgctggcttt
     3121 atgcgcaggc tccatgccca acgccaggcc ggcattattt accaggatat caatattgct
     3181 ccactcggca gggagcgatg ccagcatctc ttcaatagcg gcgcggttgc gaacgtccag
     3241 ttgggcgata tacagattat ctcccagttc gtcttttaac tcctgcaacc gctcctggcg
     3301 acggccagtg gcgataactt tatgcccttg ttgaataaaa cgacgagtaa tgcattcacc
     3361 aaaacctgcc gttgctccag ttactaaaac gatcatctca ctgttcctca acgcttttgt
     3421 gtgtcaataa catagcacgt gatgcttaag agcgggtaat actggtttgc aagattgcac
     3481 tccgttatca gtaagcctac gctatggaac acctcgcgtt caggagaaga aaatgacaac
     3541 aatgaatcct ttccttgtgc aaagcacact gccgtatctg gctcctcatt ttgatcaaat
     3601 tgccaatcat cactatcgcc cggcattcga tgagggaatg caacaaaagc gggcagaaat
     3661 tgctgccatc gcgcttaacc cgcaaacgcc tgatttcaac aatactattc tggcactgga
     3721 acaaagcgga gaattaatta cccgcgttac cagcgtcttt tttgcgatga ctgcggcgca
     3781 taccaatgat gaattacagc gtcttgatga acagttttcc gctgaactgg cggaactggc
     3841 taatgatatc tatctgaacg gtgaattatt cgcgcgggta gatgctgtct ggcagcgccg
     3901 tgaatccctg gggcttgata gtgaatccat ccgcctggtg gaggttattc atcaacgttt
     3961 tgtccttgcc ggagccaaac ttgcacaagc tgataaagca aaattaaaag tactgaatac
     4021 agaagctgcg accctgacca gccagtttaa ccagcggtta cttgcagcaa ataaatccgg
     4081 cggtctggtt gtgaacgata tcgcgcagct ggcaggaatg agtgagcaag agattgcgct
     4141 ggcggcagag gcggctcgcg agaaaggtct ggataacaaa tggctgattc cgctgctgaa
     4201 taccacccaa caaccggcgc ttgccgaaat gcgcgatcgt gcgacgcgtg aaaaactgtt
     4261 tattgcgggc tggacgcgag cggaaaaaaa tgatgccaat gatacccgcg ctatcattca
     4321 acgtctggta gagattcgcg cacagcaggc gaaactgctt ggttttcctc attatgccgc
     4381 atggaaaatc gccgatcaga tggcaaaaac gccagaagca gcactcaact ttatgcggga
     4441 aattgttcca gcggcgcgtc aacgtgcgag cgatgaatta gcctccatac aggcggttat
     4501 cgataagcag cagggcgggt ttagcgcgca gccgtgggac tgggcatttt atgcggaaca
     4561 ggtacggcgg gagaaatttg atcttgatga ggcgcagctc aagccatatt ttgaattaaa
     4621 cacggtgtta aatgaaggtg tattctggac cgcgaatcag ctcttcggta ttaagtttgt
     4681 cgaacgtttt gatattccgg tctaccatcc tgacgttcgt gtgtgggaaa tttttgatca
     4741 taatggcgtg ggactggcgt tattttacgg tgatttcttc gcccgtgatt caaaaagcgg
     4801 cggtgcatgg atgggcaatt ttgttgagca atcaacgctt aatgaaacgc atccggtaat
     4861 ttataacgtc tgcaattatc agaaacccgc tgccggtgag cctgcgttgt tactctggga
     4921 tgatgtaata accttattcc atgaatttgg tcatacgctg cacggccttt ttgcccgcca
     4981 gcgttatgcc acgctttccg gcaccaacac gccgcgtgat tttgtcgaat ttccgtcgca
     5041 aatcaacgaa cactgggcaa cgcatccgca ggtattcgct cgctacgccc ggcattatca
     5101 gagcggggca gcaatgcctg acgaactgca acagaaaatg cgtaatgcca gcctgttcaa
     5161 caaagggtat gagatgagcg aactgcttag cgccgcactt ctcgatatgc gctggcattg
     5221 cctggaagaa aacgaagcaa tgcaggatgt cgatgatttt gaattgcggg cgctggtggc
     5281 ggaaaatatg gatcttcctg ctataccgcc acgctatcgc agcagttatt tcgcccatat
     5341 ttttggtggc ggatatgctg caggttatta cgcttatctg tggacgcaaa tgttggccga
     5401 tgatggttac cagtggtttg ttgagcaggg cggattaacg cgtgaaaatg ggcagcgttt
     5461 tcgcgaggcg atcctttcca gaggtaacag cgaagatctg gaacgcctgc atcgacaatg
     5521 gcgcggtaag gctcctcaga ttatgccgat gctgcaacat cgtggcttga acatataaac
     5581 accaccaatg gctggcctgg agatatcgcc aggccagcct ggttatagca gtaattgcag
     5641 c
//
LOCUS       DAJTYF010000185         5599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTYF010000185 DAJTYF010000000
VERSION     DAJTYF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.187"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..410
                     /locus_tag="OO590_003920"
     CDS             <1..410
                     /locus_tag="OO590_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6639078.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /gene="istB"
                     /locus_tag="OO590_003921"
                     /pseudo
     CDS             442..1056
                     /gene="istB"
                     /locus_tag="OO590_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OO590_003922"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OO590_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR6639079.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWFSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1380 a         1402 c         1329 g         1488 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggacagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tcaacagcgc aggagagctg
      721 ttgcaggaac tacataaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag gcctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggtata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggtc ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagaa
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gcccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc gacgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaagaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tgctcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aagggccagt ttggatatc
//
LOCUS       DAJTYF010000186         5571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTYF010000186 DAJTYF010000000
VERSION     DAJTYF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.188"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..102
                     /locus_tag="OO590_003923"
     CDS             <1..102
                     /locus_tag="OO590_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6639080.1"
                     /translation="VLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            163..3642
                     /locus_tag="OO590_003924"
     CDS             163..3642
                     /locus_tag="OO590_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6639081.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLERWDTPKVVKGVSFLLRLTVAADDGSERLVSTGRTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDYAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKTLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTTNKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            3710..4309
                     /locus_tag="OO590_003925"
     CDS             3710..4309
                     /locus_tag="OO590_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6639082.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            4373..>5571
                     /locus_tag="OO590_003926"
     CDS             4373..>5571
                     /locus_tag="OO590_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6639083.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPWYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKTANHLSEIAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLCIRWTDARPETTEPRYRAKSLTFYGI
                     NGPIYHTRYCYWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIGGLIIAAYQG
                     EADGDKV"
BASE COUNT         1323 a         1359 c         1734 g         1155 t
ORIGIN      
        1 gttctgtacg gtgaaatgcg cgtggggtcg cgggtggtat ctcaggagat cagcacggca
       61 gacgaagggg atggtggtca ggttgtggtg attggtcgct gatgcaaaat gtttcatgtg
      121 aaaccgcctg cgggcggttt tgtcgtttat ggagcgtgag gaatgggtaa aggcagcagt
      181 aaggggcata ccccgcgcga agcgaaggac aacctgaaat ccacgcagtt gctgagtgtg
      241 attgatgcca tcagcgaagg gccggttgac ggtccggtgg atggattaaa aagcgtgctg
      301 ctgaacggta cgccggtcct ggacagcgag gggaagacaa acttttccgg tgttacggtg
      361 gtgttccgcg ccggcgagca ggagcagaca ccgccggagg ggtttgaatc ttccggctca
      421 gagactgtgc tgggtacgga agtgaaatac gacacgccga tcacccggac catcacgtcg
      481 gcaaacattg atcgtctgcg cctgaccttc ggtgtgcagg cactggtgga aaccacctca
      541 aagggggacc ggaatccgtc ggaagtccgc ctgctggttc agatacagcg taacggtggc
      601 tgggtgacgg aaaaagacat caccattaag ggcaaaacca cttcgcagta tctggcctcg
      661 gtggtggtgg gtaacctgcc gccgcgcccg tttaatatcc ggatgcgcag gatgacaccg
      721 gacagcacca cagaccagct gcagaacaaa acgctctggt cgtcgtacac cgaaatcatc
      781 gatgtgaaac agtgctaccc gaacacggcg ctggtcggcg tgcaggtgga ttcagagcag
      841 ttcggtaacc agcaggtgag tcgcaattat catcttcgcg ggcgcattct gcaggtgccg
      901 tcgaactata acccgcagac gcggcaatac agcggtatct gggacggaac gcttaagcca
      961 gcatacagca acaacatggc ctggtgtctg tgggacatgc tcactcatcc gcgctacggc
     1021 atggggaaac gtcttggtgc ggcagatgtg gacaaatggg cgctgtatgt catcggccag
     1081 tactgcgacc agtcagtgcc ggacggattt ggcggcacgg agccgcgcat cacctgtaac
     1141 gcttacctga ccacacagcg taaggcgtgg gatgttctca gtgatttctg ctcggcgatg
     1201 cgctgtatgc cggtatggaa cgggcagacg ctgacgttcg tgcaggaccg accgtcggat
     1261 aaagtgtgga cctataaccg cagtaatgtg gtgatgccgg atgatggcgc gccgttccgc
     1321 tacagcttca gcgccctgaa ggaccgccat aatgtcgttg aggtgaactg gattgatccg
     1381 gacaacggtc atgagacggc gacagagctt gtggaggaca cgcaggccat tgtccgttac
     1441 ggtcgtaacg tcacgaagat ggatgccttt ggctgtacca gccggggaca ggcgcaccgc
     1501 gccgggctgt ggctgattaa aacggaactg ctggaaacgc agaccgtgga cttcagcgtc
     1561 ggcgcagaag ggcttcgcca tgtaccgggt gatgtcattg aaatctgcga tgatgattat
     1621 gccggtatca gcaccggtgg tcgcgtactg gcggtgaaca gccagacccg gacgctgacg
     1681 ctcgaccgtg aaatcacgct gccgtcctcc ggtaccacgc tgataagcct ggttgacgga
     1741 aatggcaatc cggtcagcgt ggaggttcag tccgtcaccg acggcgtgaa ggtgaaagtg
     1801 agccgggttc ctgacggcgt tgcagaatac agcgtgtggg ggctgaagct gccgacgctg
     1861 cgccagcgcc tgttccgctg cgtgagtatc cgtgagaacg acgacggcac gtatgccatc
     1921 accgccgtgc agcatgtgcc ggaaaaagag gccatcgtgg ataacggggc acactttgac
     1981 ggtgaccggc gcggcacggt gaatggtgtc acgccgccag cggtgcagca tctgaccgcc
     2041 gaagtcaccg cagacagcgg ggaatatcag gtgctggagc gatgggacac gccgaaggtg
     2101 gtgaagggtg tgagcttcct gcttcgcctg accgtggcag cggacgacgg cagtgagcgg
     2161 ctggtcagca caggcaggac gacggaaacc acataccgct tcaggcagct ggcgctgggg
     2221 aattacagtc tgacagtccg ggcggtaaat gcccgggggc agcagggcga tccggcgtcg
     2281 gtatcgttcc ggattgcggc accggcagcg cctgtcacta ttgaactgat accggggtat
     2341 tttcagataa cggtggtccc gaaactggct gtatatgacc cgacggtgca gtttgagttc
     2401 tggttttcgg aaaagcggat tgcggatatc aggcaggttg aaaccagcgc ccgctatctt
     2461 ggcacggcgc tgtactggat agccgccagt atcaatatca ggccgggcca tgattattat
     2521 ttttacgttc gcagtgtgaa caccgttggc aaatcggcat ttgtggaggc cgtcggtcgg
     2581 gcgagcgatt atgcggaagg ttatctgagt ttttataaag ggttgatcaa taaaacgcat
     2641 ctcggcaagg agttgtggac gcagattgat aacggtcagc ttgcgccgga cctgactgaa
     2701 atcaggacgt ccattacgaa tgtcagcaat gaaatcacgc aaaccgtcaa taaaacactg
     2761 gaaaatcaga gtgctgcaat ccagcagata cagaaagttc aggttgatac aaataataac
     2821 ctgaacagca tgtgggctgt gaagctgcaa cagatgaagg acggacgcct ttatattgcg
     2881 ggtatcggtg ccggtattga gaatacgcca gcaggaatgc agagtcaggt gctgctggcg
     2941 gcagacagga ttgcgatgat taatcctgcg aatggcaaca caaagccgat gtttgttggt
     3001 cagggcgatc agatattcat gaacgacgtg ttcctgaaac gcctgacggc tccgaccatt
     3061 accagcggcg gtaatcctcc ggcattttcc ctgacacctg gcggacggct gacggcgaaa
     3121 aatgccgata tcagcggtaa cgtgaacgcg aactccggga cgctcaacaa cgtcacgact
     3181 aataagaact gtcgggttct gggaaaattg tccgcgaacc agattgaagg cgatctcgtt
     3241 aaaacagtgg gcaaaccttt cccacgggac tcccgggcac cggagaggtg gccatcaggg
     3301 accattaccg tcagggttta tgacgatcag ccgtttgatc ggcaaattgt tattcccgcg
     3361 gtggcgtttc gcggtgctaa acatgagcgg aagaataaca atatttattc gtcatgccgc
     3421 ctgatagtga agaaaaacgg tgctgaaatt tataaccgaa cgaccctgga taatacgctg
     3481 atatatacgg gtgttattga tatgcctgcc ggtcacggtc acatgacgct ggagttttct
     3541 gtatcggcat ggctggtaaa tggctggtat cccacagcaa gtatcagcga tttgctggtt
     3601 gttgtgatga agaaagccac tgcaggcatc acgattagct gaattttata acccagatac
     3661 gggcaccaga aatggtgcct tttttattgc agaaaagcga gaggtaatta tgcgtaaagt
     3721 ttgtgcagcc attttgtccg cagccatctg tctggctgta tccggtgcgc ctgcatgggc
     3781 gtctgaacat cagtccacgc tgagcgccgg gtatcttcag ccccatactg atatgccagg
     3841 cagcgatgac ctgaagggca ttaacgtgaa ataccgttat gagtttacgg acacgctggg
     3901 gctggtgacg tcattcagct atgccaacgc tgaagatgag caaaaaacgc attacagcga
     3961 tacccgctgg catgaggatt ccgtgcgtaa ccgctggttc agcgtgatgg cggggccgtc
     4021 tgtgcgcgtg aatgaatggt tcagcgcgta tgcgatggcg ggcgtggctt acagccgtgt
     4081 gtcgaccttc tccggggatt atctccgcgt aactgacaac aaggggaaaa cgcacgatgt
     4141 gctgaccggg agtgatgaca accgccacag caacacgtct ctggcgtggg gggctggtgt
     4201 gcagtttaac ccgaccgaat ccgtggccat tgaccttgct tatgaaggtt ccggcagtgg
     4261 cgactggcgc actgacggtt tcattgtggg tgtcggttat aagttctgat tagccaggta
     4321 acacagtgtt atgacagccc gctggttcag gcgggctttt tgtggggtga atatggcagt
     4381 aaagatttca ggtgtactga aagatggggc gggtaaacct gtcgtaaatt gtgcgattga
     4441 actgcgggcc agaagaacca gtccgaccgt tgtggcacac gttgttgcca cttgcgtgac
     4501 ggacaataac ggtgcttatg tgattgaggc tgagccgtgg tattacgagg ttgcgcttca
     4561 ctgtaacggc tggcagccaa cccgtgtcgg ggatattgat gtggcaccga ctgatgcacc
     4621 ggggacactg aacgcgtttc tgaatgcacc aaaggatggt gatttacgtc cggaggtgat
     4681 gaagcgcttt gaggaaatgg tggcgcaggc gcagcagagt gccggggctg cagccggaaa
     4741 cgcacagcag acggcgcagg atgtggcggc agccgcaacg gcccgtgatg atgcacaacg
     4801 ttttgcggag aaagcccgac aggatgcaac cgtcacagct gaggacagaa aggccactgc
     4861 ggaagatgtg acaagcacag gagcaaatgc agccgcagcc ggacagagcg cacaggatgc
     4921 cgcaggttat gcccgcgcag cagaacaggc caaaaatgac attgatgctg cgctgaccgg
     4981 caccctgaaa acggctaacc atctgtctga aatcgcagca gcaggcgaaa aggcacaaca
     5041 gaagtcccgg gataatctgg ggctgaaaag tgcggccacg atggaagcac agagcgacat
     5101 ttacgaccgg acaaaaggcc gtctggcgat acccggcgca ttcggctttg ggtgtgcttt
     5161 tctgcctgaa gatgttatcc gttttgacac taagagtgat ttcctggcct gggtaaggaa
     5221 tgcgctgcca ggtgaatatt ccgttgctgg cccttacggc atcatcatac ccgacacacg
     5281 gtttgaaggg gtgctctgca tccggtggac tgatgcacgc cctgagacaa cagaaccgcg
     5341 gtacagagcc aaatccctta ctttttacgg cattaacggc cccatttatc atacccgcta
     5401 ctgctactgg cccatatcca gactgactgg ctgggtgaaa ataaatataa ccacagaaga
     5461 tattatttac agaatcgtgg cgagctctgt ccgcaacaga tggggagacc ctgacattgg
     5521 cgggctgatt attgctgcgt accagggaga agctgacggt gataaagtca t
//
LOCUS       DAJTYF010000187         5537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTYF010000187 DAJTYF010000000
VERSION     DAJTYF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.189"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO590_003927"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO590_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6639084.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO590_003928"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO590_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6639085.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..>5537)
                     /gene="mdtF"
                     /locus_tag="OO590_003929"
     CDS             complement(2659..>5537)
                     /gene="mdtF"
                     /locus_tag="OO590_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HCR6639086.1"
                     /translation="STSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAVQ
                     QQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGSE
                     YAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQTR
                     LQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAGA
                     NALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLVM
                     YLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVVV
                     ENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQFS
                     ITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDST
                     RSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTTK
                     VLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTAI
                     IQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLSL
                     AAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGSSYVNDFL
                     NQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYNG
                     IPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYAI
                     SLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIGL
                     SAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHGA
                     GSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1463 a         1288 c         1302 g         1484 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atatcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaaaaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg acttatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggctgt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag tccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccaactc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagctg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccac
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaag
//
LOCUS       DAJTYF010000188         5531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTYF010000188 DAJTYF010000000
VERSION     DAJTYF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.190"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            100..1008
                     /locus_tag="OO590_003930"
     CDS             100..1008
                     /locus_tag="OO590_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6639087.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFY
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAADKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPETMIPGSTVPFISTK"
     gene            complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="OO590_003931"
     CDS             complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="OO590_003931"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6639088.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3323..4081
                     /gene="loiP"
                     /locus_tag="OO590_003932"
                     /gene_synonym="yggG"
     CDS             3323..4081
                     /gene="loiP"
                     /locus_tag="OO590_003932"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6639089.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4287..5207)
                     /gene="speB"
                     /locus_tag="OO590_003933"
     CDS             complement(4287..5207)
                     /gene="speB"
                     /locus_tag="OO590_003933"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6639090.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5343..>5531)
                     /locus_tag="OO590_003934"
     CDS             complement(5343..>5531)
                     /locus_tag="OO590_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639091.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1325 a         1482 c         1478 g         1246 t
ORIGIN      
        1 ttcgctatac ccctttatag attctgaatt ctcacctatc ggctgttttc tcctcatctc
       61 atgtgacata ttcccatact ttactcaagg aagattgtca tgaaagcacc tgtttcatta
      121 acctcatttc gcccacaaaa atctcttgct atcgccattg gcgtgcttgc cgtggttgtg
      181 ctgccatttc tctcgtatta caccgttaat gaaggtgaac gcggcatttt gctgcgctac
      241 ggaaaaatcg taaaagttgc cgagccgggg ctgggtttta agatcccttt tatggaaagc
      301 gtggagaaaa tttccacgcg taatcaggct gtggtctatc agggattaca ggcctacagc
      361 cgcgaccagc aaccggcaca aatgacagta tcagtcagct tctacattaa accttcagaa
      421 gcaggcgcgg tttataccac ctacaacact attgaagcat taaaagatcg tctaattgtc
      481 cgtcagttgc caactcagct ggagaacatt ttcggccaat ataccgccat cagcgccgta
      541 caggatcgta caaaattggt tcaggatctg caaaatgcca tgcgcaaagc cgttgttggg
      601 ccagtggtaa ttgacggggt ccagattgaa aatattgatt tttctgacgc ttatgaaaag
      661 tcgattgaaa accgaatgaa agcagaagtc gccattgcca cgcgtaagca aaatctggaa
      721 acagagaaaa tccaggcgca aatagcggta actcaggccc aggcagaagc agacagtaaa
      781 ctggcggcag acaaagcaga agcggaaact attcgcgtaa gaggcgcggc agaggctgaa
      841 actattcgtt taaaaagtgc ggctgaagca gaagcaatcc gcctgcgcgg tgaagctctg
      901 cgggataatc ccggacttgt agccctcacc actgccgaac gttgggacgg taaactgccc
      961 gaaaccatga tccccggcag cacagttccg tttatctcta ctaagtaaaa aaagggccgc
     1021 tttcgcggcc cttctcacaa aacgtcttac tgattacagc agcgcttttg ctttcgcaac
     1081 aacgttatca acagtgaagc cgaactcttc aaacagcagc tctgccggag cagattcacc
     1141 gaaggtggtc atgccgacga tagcgccgtt caggccaaca tacttgtacc agtagtcagc
     1201 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1261 agccgcatcc tgcttgtcaa atgcgtcggt agacggcatg gacaccacgc gcgctttcac
     1321 gccttcggca gtcagttttt cgtaggcagc aaccgccagt tcaacttcag aaccggtggc
     1381 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     1441 gtttgccagc tgctcttcag tacgttcctg ctgcgccagg ttctgacggg agaggataag
     1501 cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac gcgaccgcgg attcaacctg
     1561 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctctac
     1621 cggctggtga gtcgggccat cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     1681 ctggcgctgt ttcatcagcg cagccatacg tacggcgtta cgtgcgtatt ccacgaacat
     1741 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     1801 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cctgcagcat cttcgttgat
     1861 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcag agccgccgag
     1921 gaattcaggc aacagcgggc cgaacgcttc gatagcattc tgcgacgctt tacggctggc
     1981 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2041 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2101 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2161 atcccactga gcatagattt cagacgggat ttcgaacggt gcatatttcc agcccagctg
     2221 ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca ccgtgggagt cgtgagtacc
     2281 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     2341 agtcactgcg cgcgcttctt caactgcgcg tttgatggat gccgcgtcat gaccgtcgat
     2401 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gctgtgtcgt cggtgaacca
     2461 gccttcaacg tgaccatcga tagaaatacc gttgtcatcg tagaacgcaa tcagtttacc
     2521 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     2581 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     2641 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     2701 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     2761 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     2821 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     2881 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     2941 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3001 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3061 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3121 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3181 cagaaatctc tggatcatgc tcgcatgttg cgcaatctgc tcgcccgtcc gctgcgcttt
     3241 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     3301 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     3361 aacggtactg accggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     3421 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     3481 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     3541 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     3601 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     3661 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     3721 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     3781 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     3841 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     3901 tgattattct tacgatcttc tgcaccaacg cggcatcagc ccggcaggtc ttgccaccag
     3961 ctttgaaaaa ctggcgaagc tggaagaagg ccgtcaaagc tcaatgtttg acgaccatcc
     4021 tgcatccgcc gaacgtgccc agcatattcg cgatcgcatc agcgcggatg ggattaagta
     4081 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacattaatg
     4141 gcacatttgt taccttgtgc gcattgccgg atgcgatgct ggcgcatctt atccggccta
     4201 cgggtgccga acgtaggtcg gataaggcgt tcacgccgca tccgacatta atggcacgtt
     4261 ttacccgtgc gcatcgcatc tggtgcttac tcgccctttt tcgccgcctg aatatacagc
     4321 atctccagcg ccagcgtcgc cgcagccaga gcggtgattt ccgactgatc gtatgccgga
     4381 gccacttcca ctacgtccat cccaacgatg ttgagatctt tcaggccgcg taccagttta
     4441 atggcgcgat cggaggtcag gccgccaatc actggcgtac cggttcccgg cgcgaaagca
     4501 ggatccaggc agtcgatatc aaaagtcagg taaaccggca tatcacccac aatctgtttc
     4561 acctgggcga taacgtcatc cacgctgcga tcgttcacct ggcaggcgtc cagcacggta
     4621 aagccgttgt ctttatcaaa ctcggtacga ataccaatct gcacggaatg attcgggtcg
     4681 atcagacctt ctttcggcgc ggtatagaac atagtgccgt ggtcaaattc acaaccgttc
     4741 gcataggtat cggtgtgggc gtcaaagtgt accagcgcca ttttgccgaa atgcttcgca
     4801 tgagcacgca gcagcggcag cgtaacaaag tggtcaccac cgaaggagag catacgctta
     4861 ccggcagcca gcagcttctc ggcgtgcgcc tgcagctttt cgctcatctc acgggcatcg
     4921 ccaaaggcat ataccagatc gccgcagtcc acgacgttca gacgctcacg catgtcgaaa
     4981 ttccacggga agcggttgtg ttcccaggcc agattcgtcg aaacctgacg gatcgctgcc
     5041 ggaccgtggc gaccacccgc acgaccagaa gtggccatat cgaacggcac gccagtaatc
     5101 acccagtctg catcgctgtc atacggctgg aagttcatcg gcaggcgtaa aaaaccaaag
     5161 gcattggaaa ccagtgagtt atcgtattga tgacctaagg tgctcatgga tggactccta
     5221 ttacaaagtc gatataaaaa aacccttccg cgtcgttagg cccgacgagg aagggttgga
     5281 tttgcgaaac acaagtttgt gctaattatc gccttaaatg ggcgaggcat caagcgaggt
     5341 tgctatttcc gacacggtgg cggcgcataa ccattgttat ctgccggaaa ggcttgtcca
     5401 gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt
     5461 accccggtat tacgcacatc gccttgcgcc gaattggctg cgtaggaatc agcaacgttg
     5521 gcgaactgaa a
//
LOCUS       DAJTYF010000189         5465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTYF010000189 DAJTYF010000000
VERSION     DAJTYF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.191"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(296..580)
                     /locus_tag="OO590_003935"
     CDS             complement(296..580)
                     /locus_tag="OO590_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR6639092.1"
                     /translation="MLDMAGLRADILSPVKETGTLLGVVSSQAAELCGLKAGTPVVVG
                     GGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQLPITPFRMPS"
     gene            614..1420
                     /locus_tag="OO590_003936"
                     /pseudo
     CDS             614..1420
                     /locus_tag="OO590_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            1432..2455
                     /gene="lsrB"
                     /locus_tag="OO590_003937"
                     /pseudo
     CDS             1432..2455
                     /gene="lsrB"
                     /locus_tag="OO590_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            2482..3357
                     /gene="lsrF"
                     /locus_tag="OO590_003938"
     CDS             2482..3357
                     /gene="lsrF"
                     /locus_tag="OO590_003938"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR6639093.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PGVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            3381..3671
                     /gene="lsrG"
                     /locus_tag="OO590_003939"
     CDS             3381..3671
                     /gene="lsrG"
                     /locus_tag="OO590_003939"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR6639094.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            3710..4468
                     /gene="tam"
                     /locus_tag="OO590_003940"
     CDS             3710..4468
                     /gene="tam"
                     /locus_tag="OO590_003940"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR6639095.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(4472..5386)
                     /locus_tag="OO590_003941"
     CDS             complement(4472..5386)
                     /locus_tag="OO590_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR6639096.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNELPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         1379 a         1306 c         1457 g         1323 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt
      661 actgtttcaa agtggtgtgc cgatgccgct ggcgatactc ctgaccttac tgctcggcgc
      721 attgtgcggg ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat
      781 tacgcttggc acgctgtatc tgtttgccgg aagcgctctg ctgctttccg gtatggccgg
      841 agcgacgggg tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct
      901 ggatgtgctg ggactccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg
      961 gctctggctg cataaaaccc atgccggacg taatgtgttt ttgattgggc aaagcccgcg
     1021 cgtggcgctt tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac
     1081 ggggctggcg tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc
     1141 cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggggccaa
     1201 tatttatggt ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata
     1261 tttgcagcaa ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg
     1321 tgcgctgctt atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa
     1381 agagtggctg gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt
     1441 catcgcttta agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat
     1501 gtgcaggccg cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc
     1561 agcggtggca acggcgcaca acaagcgggt aaagagctga gcgttgatgt gacctacgac
     1621 gggccgacag aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcaa
     1681 ggttataacg ccattattcg tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa
     1741 acgcgccatg caacgtggtg tgagagtgct gacctgggac tctgatacta aaccggagtg
     1801 ccgctcttac tacattaatc agggaacgcc cgcccagttg ggaggtatgt tggtggatat
     1861 ggcggcgcgt caggtgaata aagacaaagc caaagtcgcg tttttctact caagccccac
     1921 cgttacggac caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc
     1981 aggctgggaa attgtcacta cgcagtttgg ctataacgat gccactaaat cgttacaaac
     2041 cgcagaagga atattaaaag cgtatagcga tctcgacgcc attatcgccc ccgatgccaa
     2101 cgccctgccc gctgccgcac aagccgcaga aaacctgaaa aatgacaaag tagcgattgt
     2161 cggattcagt acgccaaatg tgatgcgccc gtatgtagag cgcggcacgg tgaaagaatt
     2221 tggactgtgg gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg atgcattatt
     2281 gaaaaaagga tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag gtcaggttga
     2341 agtctcgcca aacagcgttc agggctatga ctacgaagcg gatggtaatg gcatcgtact
     2401 gttaccggag cgcgtgatat tcaacaaaga gaatatcggc aaatacgatt tctgatgtgc
     2461 attacttaac cggagtaagt tatggcagat ttagacgata ttaaagatgg taaagatttt
     2521 cgtaccgatc aaccgcaaca aaatatccct tttaccctga aaggttgcgg tgcgctggat
     2581 tggggaatgc agtcacgctt atcgtggata tttaatccga aaacgggtaa aactgtgatg
     2641 ctggcctttg accatggtta ttttcagggg ccgactaccg gacttgaacg cattgatata
     2701 aatatcgccc cgctgtttga acatgccgat gtattaatgt gtacgcgcgg cattttgcgc
     2761 agcgtagttc ccccggcgac caataagccg ggggtactgc gggcgtcagg tgcgaactct
     2821 attctggcgg aattaagtaa tgaagccgtg gcgttatcga tggatgatgc cgtgcgcctg
     2881 aacagttgcg cggtggcggc gcaggtttat atcggcagcg aatatgaaca tcagtcgatc
     2941 aaaaatatta ttcagctggt cgatgccgga atgaaagtgg gaatgccgac catggccgtg
     3001 actggcgtgg gcaaagatat ggtgcgcgat cagcgttatt tctcgctcgc gactcgaatc
     3061 gccgctgaaa tgggggcgca aattatcaaa acctattatg tcgaaaaagg ttttgaacgg
     3121 attgttgccg gatgtccggt acccattgtt attgctggcg gtaaaaaatt accggagcgc
     3181 gaggcgctgg aaatgtgctg gcaggctatc gatcagggcg cttctggtgt ggatatgggg
     3241 cgtaatattt tccagtctga ccatccggtg gcgatgatga aagccgtaca ggcggtggtt
     3301 caccataacg aaacggctga tcgggcatat gaactctatc tgagtgaaaa acagtaactg
     3361 cggatttaag gagaagaatt atgcacgtca cactggttga aattaacgtt catgaagaca
     3421 aggttgacga gtttatcgaa gtttttcgcc agaaccacct gggctctgta caggaagaag
     3481 gtaatttgcg cttcgatgtc ttacaggacc cggaagtgaa ttcgcgcttt tatatttacg
     3541 aagcctataa agatgaagac acagtggcat tccataaaac cacgcctcac tacaaaacct
     3601 gtgtcgcgaa actggaatct ttaatgaccg ggccgcgtaa aaaacgtctg ttcaatgggt
     3661 tgatgccgtg aggctacaat tggggtaacg cgctgacggg agtaaaaaaa tgtctgactg
     3721 gaacccctct ttatatctgc acttttccgc tgaacgatcg cgtccggcgg tggagctgct
     3781 tgccagagtg ccgctggaga atgtcgaata tgttgccgat cttggctgtg gcccaggtaa
     3841 cagcaccgcc ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc
     3901 tccggcgatg attgctgaag cgcgcagtgc tttgccagac tgccagttcg tggaagcaga
     3961 catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca
     4021 atggctgccc gaccactacg aattgtttcc tcacttggtt tctttactta atccgcaggg
     4081 cgtgctggca gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga
     4141 agttgcttgg gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc
     4201 ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacttacta
     4261 tcaccagatg ccgtcgcacc aggcgattat cgattgggtg accgccactg gattacgtcc
     4321 gtggttacag gatctgaccg agagcgaaca gcagcttttt cttacgcgct accatcagat
     4381 gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct
     4441 gtttattgtt gcccgccgta cggagtaatt atcacgtcag ctggtaatga cgatcgggaa
     4501 gaatttttgc tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt
     4561 tgcagatggc atccagcggt aactcattgt tttcagtacc gaacggatct tccagttctt
     4621 ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca
     4681 tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac
     4741 gatgcaaaat cagggtgtag gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg
     4801 ccagaaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa
     4861 gctgtccatt gcggcgctga accgccaacc attctcccat tattaacaag atacggttag
     4921 ctggagagtt cgaagccagt acacgctgaa gatctccagt cttgagataa tgagccagca
     4981 cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca
     5041 gcctggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tctcgcagta
     5101 acgaccgtga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt
     5161 acccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg
     5221 gtgcgagggt gaatttgatg cccagatgcg tgtaccaggg cagcatgaat ataacagcaa
     5281 tagaaaagag aaaattgagt agtaagcgcg aggatatctt ggataatact gagccgtgcc
     5341 agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt atcttcaggc
     5401 gtggaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacgc tacttacaaa
     5461 ttacc
//
LOCUS       DAJTYF010000190         5426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTYF010000190 DAJTYF010000000
VERSION     DAJTYF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.192"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..88)
                     /locus_tag="OO590_003942"
     CDS             complement(<1..88)
                     /locus_tag="OO590_003942"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6639097.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(250..3558)
                     /gene="icsA"
                     /locus_tag="OO590_003943"
                     /gene_synonym="virG"
     CDS             complement(250..3558)
                     /gene="icsA"
                     /locus_tag="OO590_003943"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6639098.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGSS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4087..5289
                     /gene="virA"
                     /locus_tag="OO590_003944"
     CDS             4087..5289
                     /gene="virA"
                     /locus_tag="OO590_003944"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6639099.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1683 a         1223 c          900 g         1620 t
ORIGIN      
        1 gtgcattagt ttctgcaata ccagtaatct gaggcgcata accacgaata tttggcggta
       61 gcattcgttc atcgctttct aatgcaattc ctgtgtaacg ccaagcaaca gcgcattctg
      121 gcgcatgatc tggtaaacac gtttggcatt gatcgcaggc ataccatcat gtgcacaaaa
      181 acccaccgag cttttacacg gtgggtccca gagaaatgca ggacatcaac acgccctgca
      241 tttttattat cagaaggtat atttcacacc caaaatacct tgggtatctc tgtaactgtt
      301 attacccaat tgctgagata cgtttcccca gatgcttagg ttatctgcaa gacgcccccc
      361 cacaccagtt ttgagttcaa tcacattacg gttgcctatc tggtgatttg acatgccatt
      421 cataattaca ccatattgct ttgagctcca tttccaattc acttccaaaa atggttcgaa
      481 cttacgaaga gtctccttat cgatacaact ctgactaatt aaggatgtac gcaatcccaa
      541 ttttgcttga atattattga ccccagagga tgtgaccata gtgccattgt gttcaatata
      601 gtcgtttgct tttaccccat gcctagttat ctggacttgt ggattgagat acaatgcgtt
      661 gtcaatatta ttatgagcag tccagcgtat ggttgggtag atatagccag cttccaaagc
      721 tcctgttatt cctttgctgg aatatttctc accagttaac ccatctcctt tgactgtatt
      781 attaaaccaa ctgtactgca accaagcatc catatataga cctgttcgtt ctttttcatc
      841 ctgaaaccaa gaactgtaca aaccggcagt ataaccgctc acagttcctc gtgaactata
      901 actagtatac ttattatgag ttttgctatc ctgatatcca taagctccca taatacccac
      961 atgcaagcca tcatgatcag tgaagtttgt cttcaaaata tctccaccaa tctgataaac
     1021 catcctgtta gctgtggttt ttaattgtct atcagctaaa ttatgctcat tatgtccacc
     1081 aacagtacga atccacatgg tggtttcgct gagctgacct gtagtaggat caacaatagt
     1141 gctaccgtgt cggtcataga gagaaggact aaacatcgtg ttcattgcta tcaggttagc
     1201 aatataactg ccagcttcag ggcgccatgt gtgaatacta ccttcaccca aattaagtga
     1261 agatacaagc tgtgaactag ctcggcgact actcatttga gtagactctt gattgctcat
     1321 ctgttttgat tcttgattat ccatctgact agttagatac cacttattgg tattcagtcc
     1381 agatacagtg ccctgtttca ggcgatagtc ataactacca gcaacaatac ggcctttctg
     1441 aataaaagca ttcttatcag aagaatctgt cgaaataatt tgaacacctt caagagtttt
     1501 atctccagag cctccctcat ttgtcactgc aacataggta ataccagacg catggcctgc
     1561 aatttccagc cggtcagttt tactgttgtc attgccgaga accgcgccca aagataatat
     1621 tccgcccttt ccatgccaat tacctttctg aacataggtc tgaccgacat ttgacgaact
     1681 attattgaga ataacatgac cgtttttctc cagagtcatg ttacctgtga caatgacggg
     1741 gtcaggcaaa aaaggggcat taatattatt gattagcaca gtcccactat ttaataaagt
     1801 gttaacagtt tggttcatgc ctgaaaaatt caaggtagca cctttattaa caataacacc
     1861 agcggtacgt gtcatagctt caactgtccc catttttaga atgcctgcat tgatgttggt
     1921 gaaaccagag taggtatttt tttccgccaa aatgagagtt ccctcccctg attttgttag
     1981 ggattttccg tcccagctat ttatattatg atggctgaga ttatctgcca gaatagtact
     2041 aacagtaagt tcagcgtttt ctttcagatt gaactctcca tgactgtcgc catctgtatc
     2101 attccagctc aaaccgtaga tcaatttatt ctggtcactg atatagcttt ttgtatagtc
     2161 aatagcacca gtagtgagta acggattgat gatagatata ttagaaacac tggaacctgt
     2221 tatcccttta tctgcactta gcagtttata gtccttctgc gcccagttat ttataatagc
     2281 ttccactttt acattgttgc caaatgacat atttccagaa gcttccagac tactgattcc
     2341 agctaaattt aaagaactgt tctcaccaaa agaaacgtca cctgaaacag tgaatttgtt
     2401 acctgatagt gaaacagttg tacaatcacc agcacataaa tcaccttcaa tagttggtgt
     2461 ggtcccagtg taatcattgt tgataatttt cagaatggat gaattgttaa cctgtacatc
     2521 accagtaatg acagaacctt catggagtat taatgaattg tttccaccag tgatgtggat
     2581 agcattacct gctacatttt gtgttccatc atcttcttta cctgaaatta tatatccgct
     2641 attagcaatg gtcatattgc taccatttat agcatccccc caatgtccac cattaccgcc
     2701 taaaatagct ccattgttga ttacagacag gttatctcct gtgatagcat taccaccgta
     2761 gccatcataa ccataaccgt aagcagcacc tccgttgcct ccagcgaacg tgcccttatt
     2821 gattacagag aggctgcttc ctgtgatggc atcacctcca ttaccgccat caccctcacc
     2881 ataattgtta cctccattac caccggaaat agtccctttg ttgattatgg gtagattgac
     2941 tcctgtgacg gcatcacctc cattcccccc atcactatca ctataactat cgccaccatg
     3001 tccacctgaa ataatttctc catttattat aaacagattg tctcctgtaa cagcctcacc
     3061 accatcaccg ttatggtcag caccgctacc gccgctacca ccaagaatca tgccttgatt
     3121 gattatagac aagtcagatc cggtaataga gtcaccacca ttaccgccac aactattacc
     3181 atcattatta tcaccagcac caccatgacc tgcgctaatc ataatatcag agttaatccg
     3241 tagcttagtt ctagatgcat gagaggggtt ggaagaagtt atataccacg catccattcc
     3301 atcttcacca gctcccaagg gagaaagtcc atcaacaggt gttaataatt tttcataatt
     3361 gtcctctgaa aaatgaagtt cttgagtacc cgaaagagga gtagcaaaag ctattggccc
     3421 cccgagcaac agggatgcac caacaacaaa aggagaaaaa gacaatttgc atgttttttc
     3481 ctttaccgta ggtaattctc cggccccccc ctgtgaacat tgggtcatat tacaaaaaaa
     3541 tttgtgaatt tgattcatgc actatattat cagtaagtgg ttgataaacc cctgaaaaga
     3601 ttccgtttta tccggaataa agggacgata tatgcaaaaa catattaaac aaagcctcaa
     3661 ccaaaacatg agagctgtgt tatatatcaa tgcaacggga gaaatttacc tgtgtcttgc
     3721 tttgaaaaaa agaggggaaa agctatatgt tcgataataa attaataatt ttactatgat
     3781 agtctgcttc agaccgcaac ttttcagtcc tttctgtacg cttgcccaaa gtttaaccac
     3841 tttgtttttc tatacaccca atggttctat ctgccccttg atgttaatcc ccaaagccaa
     3901 taacatgaat gtattcatat cacaactttc ctaccagact ctccaatgca ataactgcat
     3961 aattgttttt agtggaaatg tatcgttttc ttaaagagaa gaataacatt ccattttatt
     4021 atgttcccaa tttaattata ttaatatata ttggcattaa taggaaaata catcaggaga
     4081 aatcaaatgc agacatcaaa cataactaac catgaaagaa atgactcatc atggatgtca
     4141 actgtcaaat caactacaga agtaagttgg aataaattga gtttttgtga cgttttactg
     4201 aaaataataa ctttcggcat atattctcca cacgaaactc tagccgaaaa atatagcgaa
     4261 aaaaaactaa tggatagttt ctcaccatca ctatcacaag ataaaatgga cggagagttt
     4321 gcccacgcaa acatagatgg catttcaata cggctatgcc ttaacaaagg catatgttcg
     4381 gttttttatc ttgatggaga taaaatacaa tccactcaac tttcatcaaa agaatataat
     4441 aacttgttaa gttcactccc ccccaaacaa ttcaacttag gaaaagtaca tactattaca
     4501 gctccggtat cagggaattt taaaactcac aagcctgcac cagaagttat tgaaacagcc
     4561 attaattgct gtacatcgat aataccaaac gatgattact tccacgttaa agacactgat
     4621 tttaatagtg tttggcatga tatttacaga gatattcgag catctgacag caatagtaca
     4681 aaaatatatt tcaacaatat tgaaatacca ctaaaattaa ttgcagatct aattaatgag
     4741 ttaggaatta atgaattcat tgatagcaaa aaagagcttc aaatgctaag ttacaaccaa
     4801 gtaaataaaa taatcaattc aaacttcccc caacaagacc tatgttttca aacagaaaaa
     4861 cttttgttta cttcactctt tcaagaccct gcatttatat ctgcactaac atctgcattc
     4921 tggcaatctc ttcacatcac gtcttcctct gtggaacaca tatatgccca aattatgtcc
     4981 gaaaacatag aaaacaggct taatttcatg cctgaacaac gagttattaa caattgtgga
     5041 catattataa aaatcaacgc agttgtccct aaaaacgaca ctgcaatctc tgcctctggg
     5101 gggagggctt acgaagttag ctcatcaata ttaccatcgc acataacatg taatggtgtt
     5161 ggtattaata aaatagaaac aagctatctc gttcatgctg gaacacttcc aagcagtgaa
     5221 gggcttcgca atgctatccc tccagaaagt cgtcaagtat catttgccat catatctcct
     5281 gatgtttaag ccaatattat attggctgcc agactgtaaa aaaacgttct tcagaacagc
     5341 tctataaact attaaaccaa agctaaaagg atggggacca atagaatctc agcccatcct
     5401 ttttatatca ttatgaaccg ccccgg
//
LOCUS       DAJTYF010000191         5425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTYF010000191 DAJTYF010000000
VERSION     DAJTYF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.193"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..92)
                     /gene="yjbD"
                     /locus_tag="OO590_003945"
     CDS             complement(<1..92)
                     /gene="yjbD"
                     /locus_tag="OO590_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6639100.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(345..1694)
                     /gene="lysC"
                     /locus_tag="OO590_003946"
     CDS             complement(345..1694)
                     /gene="lysC"
                     /locus_tag="OO590_003946"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6639101.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2219..3868
                     /gene="pgi"
                     /locus_tag="OO590_003947"
     CDS             2219..3868
                     /gene="pgi"
                     /locus_tag="OO590_003947"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6639102.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4330..4572
                     /locus_tag="OO590_003948"
     CDS             4330..4572
                     /locus_tag="OO590_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639103.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            4686..5324
                     /locus_tag="OO590_003949"
     CDS             4686..5324
                     /locus_tag="OO590_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6639104.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
BASE COUNT         1374 a         1396 c         1368 g         1287 t
ORIGIN      
        1 ccatgcgcaa tccgcgcccg atccagcagt gttttaagtt cacgctgctc gcgttcagtc
       61 atctcttttt gggtaatacg ggggagtgcc atgatggtgc cctcagtgag ccagagagtt
      121 cagtgtacgg gaattcgttt gcgagcagaa ctgataatat tcattttgcc ttctcttaac
      181 tatttatctc tttcgataat taattagaga tgcagataaa aaaattaaag gcaattattc
      241 tccgataaag acgttggtta acagagtaca ggctcgttta ttgatgagca tagtgacaag
      301 aaaatcaata cggcccgaaa tatagcttcc aggccataca gtatttactc aaacaaatta
      361 ctatgcagtt tttgcaccac ctgctcggca tcttcgccgg gcaccaggaa gcacaggtta
      421 tggctggatg cgccgtaaca aatcatgcga atgttgaacg gttccagtac gccgaatacc
      481 tctttgccaa cgccgcaggc ttttgacagg tcattgccaa tcaacgcgac cagcgccaga
      541 ccttcttcca cctccacccg gcacagtgcg gaaagctcca tcagcagaga ttgcgtcagc
      601 aacgtatcgc cagtggaggt tgaaccggtg gtatcaaggg ttaatgccac gctcacttct
      661 gacgtggtga ttaagtctac cgaaatatta tgccgcgcga ggatgccgaa aacttccgcg
      721 aggaagccgc gagaatgcag catattcagg ctgtgcaaag tgagcagagt ctgattgcga
      781 cgaagcgcca gcgcgcggaa cagcggcgga ttttcagttt tattgcacac cagcgtacca
      841 cctgcgcgtg ggtctttgct ggagccgaca aagaccggga tatcgctgcg tactgcgggt
      901 agcaacgttg ccggatgcag tacttttgca ccaaaagttg ccatctctgc cgcttctgca
      961 aacgcgattt catcaatgcg ttttgctgcg gaaactacgc gtggatcggt ggtgtagatg
     1021 cccgggacgt cggtccagat atcaacacga gatgcgtgta aagcctccgc cagcaaggct
     1081 gccgtataat cgctgcctcc acggccaagc gtcgttgtac gacctttatt ttcgctaccg
     1141 ataaatccct gggtgatcac taagccttca ttgaggcgtg ggagcagctg cagcgcggcc
     1201 agttccgcca gcgcggctac atctggctct gcacgaccaa atcggtcgtt ggtacgcatc
     1261 actttacgta catcaaacca ctgtgcctga acatcgcgtt cgcgcaggat ttcgacaaac
     1321 agcagggtcg acatcagctc gccgtggctg accagctcat ctgtcagcgc cggagacgtt
     1381 gccagcgccg ccgcttctgc cagaacagta atgttctcca gcagacgttc aatctcttca
     1441 cggataacgt tcgggtaacg cagacgttcc agaatggcaa actggatgtt gcgaatagcg
     1501 tcgagttttt cgaatcgctc gccaggttcc agcccttccg ctaaagcgac cagcagatta
     1561 gtgataccag cagaagccga gaggactact aaacgcacgt tggcatcaga aagcacaata
     1621 tcagcgctgc ggttcatggc gtcaaaatca gctacgctgg taccgccaaa tttggagaca
     1681 acaatttcag acataactac ctcatgtcag gggatccatt ttcagccttg gcacaaggga
     1741 agagcggaag acgggtgggc gcagagcgat acttcgctac tattttcacc cagaagtgct
     1801 ccaccacttg cgaaacgccc gactgcgaac gcttctggtg acaacccagg ggattcagcc
     1861 cctgtagccg atgatgaacg tggccagccg ttcaatcacc tcggcgatgc accccctcag
     1921 gtgttatcac aggactggct cctccaacac cgttacttgg gcaacgcgcc tcttctggcc
     1981 tgcgctagcg caggtagtac atttataaat aaagggtgag cggggcggtt gtcaacgacg
     2041 gggtcatgcg gatttttcat ccactcctgg cggtcagtag ttcagctaat aaatgcttca
     2101 ctgcgctaag ggtttacact caacattaca ctaacggcac taaaaccatc acatttttct
     2161 gtgactggcg ctacaatctt ccaaagtcac aattctcaaa atcagaagag tattgctaat
     2221 gaaaaacatc aatccaacgc agaccgctgc ctggcaggca ctacagaaac acttcgatga
     2281 aatgaaagac gttacgatcg ccgatctttt tgctaaagac ggcgatcgtt tttctaagtt
     2341 ctccgcaacc ttcgacgatc agatgctggt ggattactcc aaaaaccgca tcactgaaga
     2401 gacgctggcg aaattacagg atctggcgaa agagtgcgat ctggcgggcg cgattaagtc
     2461 gatgttctct ggcgagaaga tcaaccgcac tgaaaaccgc gccgtgctgc acgtagcgct
     2521 gcgtaatcgt agcaataccc cgattctggt tgatggcaaa gatgttatgc cagaagtcaa
     2581 cgcggtgctg gagaagatga aaaccttctc ggaagcgatt atctccggtg agtggaaggg
     2641 ttataccggc aaagcaatca ctgacgtagt gaacatcggg atcggcggtt ctgacctcgg
     2701 cccgtacatg gtgaccgaag cactgcgtcc gtacaaaaac cacctgaaca tgcactttgt
     2761 ttctaacgtc gatgggactc acatcgcgga agtgctgaaa aaagtaaacc cggaaaccac
     2821 gctgttcttg gtagcatcta aaaccttcac cactcaggaa accatgacca acgcccatag
     2881 cgcgcgtgac tggttcctga aagcggcagg tgatgaaaaa cacgttgcga aacattttgc
     2941 ggcgctctca accaatgcca aagccgttgg cgagtttggt attgatactg ccaacatgtt
     3001 cgagttctgg gactgggtcg gcggccgtta ctctttgtgg tcagcgattg gcctgtcgat
     3061 tgttctctcc atcggctttg ataacttcgt tgaactgctt tctggcgcac acgcaatgga
     3121 caagcatttc tccaccacgc ctgccgagaa aaacctgcct gtcctgctgg cgctgattgg
     3181 catctggtac aacaatttct ttggtgcgga aactgaagcg attctgccgt atgaccagta
     3241 tatgcaccgt ttcgcggcgt acttccagca gggcaatatg gagtccaacg gtaagtatgt
     3301 tgaccgtaac ggtaacgttg tggattacca gactggcccg attatctggg gtgaaccagg
     3361 cactaacggt cagcacgcgt tctaccagct gatccaccag ggaaccaaaa tggtaccgtg
     3421 cgatttcatc gccccggcta tcacccataa cccgctctct gatcaccacc agaaactgct
     3481 gtctaacttc ttcgcccaga ccgaagcgct ggcgtttggt aaatcccgcg aagtggttga
     3541 gcaggaatat cgcgatcagg gtaaagatcc ggcaacgctt gactacgtgg tgccgttcaa
     3601 agtgttcgaa ggtaaccgcc cgaccaactc catcctgctg cgcgaaatca ctccgttcag
     3661 cctgggtgcg ttgattgcgc tgtatgagca caaaatcttt actcagggcg tgatcctgaa
     3721 catcttcacc ttcgaccagt ggggcgtgga gctgggtaaa caactggcaa accgtattct
     3781 gccagagctg aaagatgata aagaaatcag cagccacgat agctcgacca atggtctgat
     3841 taaccgctat aaagcgtggc gcggttaatc atcatgcgtg cctgatgccg atatgtaggc
     3901 cggataaggc gtttacgccg catccggcaa ccggagcctg atgccaatat cgacattaat
     3961 gcatagtatg aggagttaaa taaccttaaa aacaatgccg acaataagtc ggcattgttt
     4021 tatcagataa atccccttgt ctgtgattta acggaaatca taccgtgagg ttaatcctaa
     4081 aatagatttt taatcgttgt ttatttcgga gaatacgcag attaattact tttgttttta
     4141 ttttaagttt atgattttta ttgttattta aatataagtt gaaacttata tttgatattc
     4201 attccaatta tcctaaaact ccatcgctaa ttccccgcgc cgtaattcgc atgctttagt
     4261 tgtgtatact cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat tcaatgaagg
     4321 gaaattgtta tgaaaaaagt tctgtatggc atttttgcca tatctgcgct tgcggcgact
     4381 tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag agtcggcagc aacgtcggtt
     4441 tcggcgggga gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt gggtgtcgcg
     4501 ctcgcggtaa ccggtggcgg tgatggttct aataccggga ccaccacaac cacgaccacc
     4561 agtacccagt aataaagtat gtatccccaa aataatttga gtcattgcat ctgtggcttg
     4621 aagtatgaag ggatttacca taaccacact ccggtgtggt tattctgccc ctctggagaa
     4681 gagttgtgaa gcgacctgca ctcattctta tctgcctgct attacaggcc tgttcagcca
     4741 cgactaaaga gctgggcaat tcactgtggg acagtctgtt cggcacgcca ggcgtacagc
     4801 tgacggacga tgatattcaa aatatgccct acgccagcca gtacatgcag cttaatggcg
     4861 ggccgcaatt gtttgtggtg ctggccttcg ctgaagacgg acaacaaaaa tgggtcactc
     4921 aggatcaagc cactcttgtc acacaacatg gtcgtctggt gaagactttg cttggcggcg
     4981 acaacctgat tgaagtgaat aaccttgccg ccgacccgct gattaaaccc gcacaaattg
     5041 ttgatggtgc aacatggacg cgcacgatgg gctggaccga gtaccagcag gtacgctacg
     5101 ccaccgcacg ctcagtcttc aaatgggatg gcaccgatac cgtcaaggtc ggcagcgatg
     5161 aaaccctggt tcgcgtgctg gacgaagaag tctccaccga ccaggcgcgc tggcataacc
     5221 gctactggat cgacagcgaa gggcaaattc gccagtcgga acagtatctc ggcgcggatt
     5281 atttcccggt gaaaaccact ctcatcaagg cggcaaaaca atgattaaac aaactattgt
     5341 cgcgttgctt ttgagtgtgg gagcgtcatc ggtcttcgcg gcaggaaccg tcaaggtgtt
     5401 cagcaatggc agcagtgagg ccaaa
//
LOCUS       DAJTYF010000192         5412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTYF010000192 DAJTYF010000000
VERSION     DAJTYF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.194"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..133)
                     /locus_tag="OO590_003950"
     CDS             complement(<1..133)
                     /locus_tag="OO590_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6639105.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     "
     gene            complement(144..377)
                     /locus_tag="OO590_003951"
     CDS             complement(144..377)
                     /locus_tag="OO590_003951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639106.1"
                     /translation="MSKEYTLSVPPFSLNTLGVDMYNNSSYIRKSYIDIPMYNRNLCD
                     FHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            complement(374..1645)
                     /locus_tag="OO590_003952"
     CDS             complement(374..1645)
                     /locus_tag="OO590_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6639107.1"
                     /translation="MWSGKVILLSLGLSLASFCHASTDIDQLYERIVNKDQTALKELK
                     KLADTNNTQALVLLGFIYEHGVTVSVDIPKAIQWYEKACNQGGEYGCYNARYFFQYGK
                     GVTLDVTRAQKYASKSKTDDLNIPPDLMNKIINQAYELKSGAETDKTKRASLIEFVSR
                     YVGQAPENDLLFWGRIGFSKPEILRLATLWAQEGDPEMDYIVGNLYLDDFSPSDDRRS
                     LDTIKKALQWFRTAAEKGYPMAQYTMGDTYENGSAGLKIDAVEAKKWYELAANKNDEK
                     ALVALGNIYYSGLTGEVDYSKASMLFDKAEQQGAKKAALWLSWMYYNGLGETLDCDKV
                     WDYYEKGAGKYSMKFNKDEYLTRCNQDQKTRETQGDTLPYITLKHYGTYGFNANEKPK
                     ICDVSIEINADKISSITNLQLTLELKGGGRE"
     gene            2039..3409
                     /locus_tag="OO590_003953"
     CDS             2039..3409
                     /locus_tag="OO590_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6639108.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFIVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
     gene            3591..5412
                     /locus_tag="OO590_003954"
                     /pseudo
     CDS             3591..5412
                     /locus_tag="OO590_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
BASE COUNT         1671 a         1128 c         1045 g         1568 t
ORIGIN      
        1 gcagtgtatt tataacatcg ggagcagatt taccaccgta tatgctgtca tacaattcat
       61 gcgctttctt acttgccacc acgcctgatg aaactatcag cattaaagaa aataatattc
      121 tattaggcaa cataaaatat ctcttattta tcttgtacct gaatatgctc ttcttttaat
      181 aaatcacgtt gttttccatt cagtatcgct gtcgcagatt taatagaata agtcaaatca
      241 tgaaaatcgc ataaattacg attatacatt gggatgtcta tatagctttt tcttatataa
      301 ctggaattat tatacatatc cacaccgagc gtattcaaac taaaaggagg gacagaaaga
      361 gtatattctt tactcactcc ctcccccccc cttttagctc aagcgtcaac tgcaaattag
      421 ttatactact aattttatct gcattaattt caatactgac atcgcatatt ttgggttttt
      481 catttgcgtt aaagccatag gtcccgtaat gttttaacgt gatgtaaggt aaagtatcac
      541 cctgtgtttc tcgggttttc tgatcttgat tacaacgggt taaatattca tccttattga
      601 atttcattga atatttcccc gcaccttttt cgtaataatc ccatacctta tcacaatcaa
      661 gcgtttcacc taatccgttg taatacatcc aacttaacca caatgcggct ttttttgccc
      721 cttgctgttc agccttgtca aaaagcatgc tcgctttaga ataatcaacc tcaccggtca
      781 aaccagaata gtatatattc cccagggcca ctaatgcttt ttcgtcattc ttatttgcag
      841 ctaattcgta ccattttttg gcttcgacag catcaatctt taaaccagca ctgccatttt
      901 cgtaggtatc gcccatggta tactgcgcca tcggataacc tttttctgcc gcagtacgaa
      961 accattgcag tgctttcttt atggtgtcta aagaacgtct atcatcagaa ggtgaaaaat
     1021 catcgagata aagatttccg acaatataat ccatttcggg gtcgccttcc tgcgcccata
     1081 gagtcgccag acgcagaatt tcaggtttac tgaaaccaat tcgcccccaa aaaagcaaat
     1141 cattctcggg tgcctgaccg acataacgac tgacaaattc aattaaactc gcccgtttcg
     1201 ttttgtctgt ctcagccccg gatttcaatt cataggcctg attgattatt ttattcatca
     1261 aatcaggcgg aatattaaga tcgtcagttt tcgacttact ggcatacttc tgtgctcgtg
     1321 tcacatctag ggtgacgcct ttaccatatt gaaagaaata acgtgcatta taacaaccgt
     1381 attcgccccc ctggttacag gctttctcat accactgaat cgcctttggg atgtcaacag
     1441 atactgtcac tccgtgttca taaataaatc ccagtagcac tagcgcctgc gtgttattgg
     1501 tatcagctaa ttttttcagt tcttttaacg ccgtctgatc tttattgaca atacgttcat
     1561 acagttgatc aatatctgtt gaagcatgac aaaaagaagc cagagataaa cccaatgata
     1621 ataaaataac tttaccagac cacatacatt cctccttgat atattcaacc accaaacatc
     1681 cacctcatga ataaatacgc tgccataacg gcccaggtaa taagtaaaca tttaagctga
     1741 aacagcataa ccagtgcctc caacgcttct atttattgcg ataaaataac accatccttt
     1801 gataaaaagt cacacgccca tcaataaatc caaaaataat gtcattagtt gcaggataaa
     1861 atcatttgag cttttcattt gttatattta acgcatgatg tattaatggc aagaatatga
     1921 gagaaactca tacctgacgt aaaaatcact ctcttatgta atatttactt attaacactc
     1981 tgtttaccta acatgcgctc cgcactacac tatgtgggca attttttaag aggtggtaat
     2041 gaacgattat aaaatgacgc caggtgagcg gcgcgcgacc tggggtttag ggaccgtatt
     2101 ttcattgcgc atgctgggca tgtttattgt actgccggtt ctgactacgt acggtatggc
     2161 actgcaaggc gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgaccca
     2221 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgaa aaccattaat
     2281 tgtcggtggg ctggcagtgt ttgccgccgg tagcgttatc gctgcgcttt ctgactccat
     2341 ctggggaatt attctgggcc gggcgctaca aggctccggt gcgattgccg ctgccgttat
     2401 ggcgctgctt tccgatctca cgcgcgaaca aaaccgtacc aaagcaatgg cgtttatcgg
     2461 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
     2521 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacgaccg gcattgcgtt
     2581 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
     2641 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
     2701 tatgtgtctg catattttgc tgatgtcgac gtttgttgcc ctgcccggac aactggctga
     2761 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacaatgc taatcgcctt
     2821 tggctcggtc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
     2881 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cgcaaacgca
     2941 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     3001 cctgctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     3061 tgtttactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     3121 tgacggcatg tttgacggtc agcgggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     3181 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     3241 cccagaaaac gtaatggcag atgaaaaact ggaaaaatgc ttactgacaa cgcctggtgt
     3301 caaagatgta ctgctggcga agttagaaca ttgcgtctat gtgaaagtag acagtaaaat
     3361 caccaatcgt tttgaaatag agcgagtaat caacgaagta tcttattaaa taataagcac
     3421 ggtgctattc atgaaaaata aaatagcacc gtacttcgta acaccataat cctttaaacc
     3481 ctccattatt aatacttcgc ttaattccat ataactcgac gattacatta gaagtaaact
     3541 atacttttta tgaaatatca gggataaatt attcacatgg aaaatacatc atgtacaaat
     3601 cattatttac tattatcatt ctactgtata gcgctagttc atatgcaaaa agcgaatccc
     3661 ctcccgcaag cactataact ttcattgcac aaatcgatca tgttgagcta aataaaacag
     3721 cattatctca tgatatgaaa ctcgttcttg ccgatcgttt ttatttcaaa accagtaatc
     3781 cttgcgaaac aaattcaatt tctgctcgac cagaatcaat ggatattact gacaaagcct
     3841 atgccaacaa attaaaaaaa ttagaaactc cttttcttga tgatcgctat ttctttatga
     3901 ccatcaatca gtgtgacggt aatggcactc ccatgctgac agacataaaa gtatgtacaa
     3961 cagaattatg tggtgctgaa tatacaaaga aaatgtctac tgtatggctc gatgaaactt
     4021 taaaaccaac tgctaaacgt ctggcaattt attttattca atctccttta ccctatgacg
     4081 aggagaaaaa acaatggaaa gtcaccggat ggtatatctc atcaactgat aatcagtctt
     4141 caactaaaca gattgcattt gaaggatata cagacacaga taacttctcc gattctaaat
     4201 ttatttcttc atttaaatcc tggtttgaaa gtggaaagat aaaaaacatt gctaactata
     4261 atccaaaagg agaaagagat ggaaaatacg aaagttatta caatagcaat ggtaacgtct
     4321 atgaatctct aagtttttct aatggtctta tcaacggtga gtttattaca tattatgaaa
     4381 atggggccat tgaaagtaaa cgtcatttta ataacgacaa aattaaagat ggtgaatgcc
     4441 ctcattttta tgaagatggg aaaatcaaac agcaacatag ctatctgaat agtaaactcg
     4501 atggtcctgc atatgaatat tatcctgatg gcaaactcat gcaggaaaat ttctatcagc
     4561 aaagtgtgtt gattggaaac gataccagtt actaccaatc cggaaaaata atctctatac
     4621 acaatagaaa ttcccgtggt cagtatgatg gagctaacga acgttatagt gaagagggta
     4681 agttacagtc taaatccgtc tataaagacg gaaaacaaat ttctgttcaa acctggtatg
     4741 aaaatgggca gaaagaagag gagaaacatt ttgatgagca aggccaatta aatggtctgg
     4801 ttaagcaatg gtataaaaat ggtaatcttg caaaatcaca aaactataaa catgacatac
     4861 tcgatggtaa cagtgaagag tggtacgaaa atggcattcc tgaaagtttg tacccttata
     4921 agaatggaaa aaccgacggc attgccaaat catggaataa gtatggtaaa ttaacctatt
     4981 caatcgaata caaaaatagc gtagagaatg gggcatatcg taactggagc aaaaacacag
     5041 gtaaactaac caaagaaacc ctgtacgtca atggtatcag acagggagtg gaaaaagagt
     5101 ttaacgatcg cacaggtaaa ctattaacct ccacgcaata tgttaataat aaaagacatg
     5161 gtacggagga aacttatgat caaaacggaa taaaatatat tacatgctat caaaatgatc
     5221 agaaattatc gtctttagac aatccaacac aaataaaaga taatgcaaca acaggcgatt
     5281 ccagtgctca atttgcatta gggaaatacg aattcatctg tgcaaatata gatgaaggga
     5341 ttaaatggtt aaccaaatct gcagagcaaa aaaaacactg atgcgatata ctttttagct
     5401 acagcctaca aa
//
LOCUS       DAJTYF010000193         5356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTYF010000193 DAJTYF010000000
VERSION     DAJTYF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.195"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..638
                     /locus_tag="OO590_003955"
     CDS             <1..638
                     /locus_tag="OO590_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR6639109.1"
                     /translation="GACIVLMELMMMVQLWWSVWLCWPVLSLLIVGRGCGGLGAITTG
                     KDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAV
                     LDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALG
                     LSRSALVVAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(692..1297)
                     /gene="azoR"
                     /locus_tag="OO590_003956"
     CDS             complement(692..1297)
                     /gene="azoR"
                     /locus_tag="OO590_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6639110.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1498..>5356
                     /gene="hrpA"
                     /locus_tag="OO590_003957"
     CDS             1498..>5356
                     /gene="hrpA"
                     /locus_tag="OO590_003957"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6639111.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLSFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPI"
BASE COUNT         1309 a         1321 c         1503 g         1223 t
ORIGIN      
        1 taggcgcgtg cattgtgttg atggagctaa tgatgatggt tcagttatgg tggtcagtct
       61 ggttatgttg gccagtatta tcgctactca ttgttggccg tgggtgcggt gggcttggcg
      121 cgataacaac agggaaagat agtcagggga aactcccgcc cgccgtttac tggctgacat
      181 tgccctggcg catcgggatg tggctatcta tgcgttggtt ttgtcgtcgc ctggagccgg
      241 tgagcaaaat gactgctggt gtttatttaa gggcgtttcc acgacatatt ccggcacaga
      301 atgcggttct ggacgtcacc tttgaattcc ctcgtggacg agcgacaaaa gatcgactct
      361 atttctgtgt accgatgttg gatctggtgg ttccggaaga gggggagctc cgacaggccg
      421 tggcgatgct ggaaacatta cgcgaagagc aaggcagcgt tctggtccat tgcgcattgg
      481 gattatcgcg cagtgcgctg gtagtggcgg catggctgtt atgttacgga cactgtaaaa
      541 cagttgatga agcgattagc tttattcgag ccagacgctc gcatattgtg cttaaggaag
      601 agcacaaagc gatgttgaaa ttatgggaaa acaggtaagt ggattgagat gtggactgaa
      661 tatctacagt ccacatcaag accgtgtccg gttatgcagc aacaatgctg tcgatggctg
      721 cttttgcgtc agactgtgct ttcgctgcca tttccggacc gtatgcgatc ccttcggcga
      781 agacaaattt cacatcggta atgccgataa agccgaggaa cgtggacaga tacggcgtca
      841 ccaggtccgt tggtccatct ttgtggatcc caccgcggct ggtaataacg atggcttttt
      901 tacccgttac cagaccttcc ggaccgttct cggtatagcg gaaagtaacg cctgcgcgtg
      961 caaccaggtc aaaatagttt ttcaactgag ttgagatgtt gaagttatac attggtgccg
     1021 caataacgat aacgtcgtgg gctttcagct cggcaatcag ctcatcggaa agcgccagag
     1081 cttcctgctg acgcggagtc agcggcgcat cgctcggacg cagagcgcca accagttcgc
     1141 catccagtac cggaatcgga tttgcagcta ggtcgcgaac ggtgatttca tcagcggagt
     1201 gcttttcgcg ccattgttca acaaaataat cggacaactg attagactga gagtaccctg
     1261 ccaggatgct ggatttaaga actaatacct tgctcatggt gtttccttat agatgtttga
     1321 atggacgatg ccccgttgct tgctgacact ttattcacaa tcctgccaca gagatagcgc
     1381 aataaatcga agtctatgtt cgaatttatt gaacaacgca tagaaagccg cgatgtggta
     1441 ctctatatct atcatttaaa agaaaattaa tcaggcagac tactgcccac taacgttatg
     1501 acagaacaac aaaaattgac ctttactgcc ttgcaacagc ggctggattc gctgatgctg
     1561 cgcgacagac tgagtttttc tcgccgtctg cattgtgtta agaaggttaa aaatcctgat
     1621 gcacaacagg ccattttcca ggagatggcg aaagagattg accaggcggc agggaaagtc
     1681 ctgctgcgtg aagcggcacg accggaaatc acttatcctg acaatttacc ggttagtcag
     1741 aaaaaacagg acatactcga agcgattcgt gatcaccagg tggtgatcgt cgccggggaa
     1801 accggctccg gtaaaacgac tcagctaccg aaaatctgta tggagctggg gcgcggtatt
     1861 aaagggctga tcggtcatac ccagccgcgt cgcctggcgg cacgaacggt ggccaaccgt
     1921 attgcggaag agctgaaaac ggagccgggc ggttgcatcg gttacaaagt gcgtttcagc
     1981 gatcacgtaa gtgataacac gatggtcaag ctgatgaccg acgggatcct gctggcggag
     2041 atccagcaag accgcctgct gatgcagtac gacaccatca ttattgacga agcgcacgaa
     2101 cgcagcctga atatcgattt tctgctcggc tatttgaaag agttgctgcc gcggcgtcct
     2161 gacctaaaaa tcattatcac ttccgcgact atcgacccgg aacgcttttc gcgccacttt
     2221 aataatgcgc cgattattga agtctccggt cggacctatc cggtggaagt gcgctatcgc
     2281 ccgattgttg aagaagccga tgacaccgag cgcgatcagt tgcaggcgat ttttgacgcc
     2341 gtagacgaac taagccagga aagccccggc gacattctga tctttatgag cggcgagcgg
     2401 gaaattcgcg ataccgccga tgcgctgaac aagctgaact tacgccatac cgaaatcttg
     2461 ccgctttatg cgcggctttc gaacagcgaa cagaaccgcg tgttccagtc gcacagcgga
     2521 cggtgcattg tgctggcgac caacgtcgcg gaaacctcgc tgaccgtacc ggggattaaa
     2581 tacgtcatcg accccggtac agcgcgtatc agccgctaca gctatcgcac caaagtgcag
     2641 cgtttgccga ttgaacccat ttcgcaggcg tctgccaatc agcgtaaagg ccgctgtggt
     2701 cgtgtgtccg aagggatctg tattcgtctc tattccgaag acgatttcct ctcgcgcccg
     2761 gagtttaccg atccggagat tctgcgtacc aacctggcct cggttatttt gcagatgacc
     2821 gcgctggggc tgggcgatat cgctgcgttc ccgtttgtcg aagcaccgga taaacgcaat
     2881 atccaggatg gcgtgcgtct gctcgaagaa ctgggcgcga tcaccacgga tgaacaggcc
     2941 agcgcctata aactgacgcc gctcggtcgc cagctctcgc agttgcctgt cgacccacgt
     3001 ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc
     3061 acgtccgcgc tctccatcca ggatccgcgc gaacgtccga tggacaaaca gcaggcatcg
     3121 gacgaaaaac atcgtcgctt ccacgacaaa gaatccgact tcctcgcgtt tgtaaatctg
     3181 tggaattatc ttggcgagca gcaaaaggcg ctttcttcca acgccttccg tcgcctgtgt
     3241 cgtaccgatt acctcaacta tctgcgcgtg cgcgaatggc aggatatcta cacccagttg
     3301 cgccaggtgg tgaaagaact tggcattccg gtcaacagcg aaccggcgga gtatcgtgaa
     3361 attcacatcg ccttactgac cggtttgctt tcccatatcg gcatgaaaga tgccgataaa
     3421 caggaatata ccggtgcacg taacgcgcgt ttctccatct tccccggttc tggtttattc
     3481 aaaaagccgc cgaaatgggt aatggtggcg gaactggtag aaaccagccg cctgtggggg
     3541 cgcattgctg cgcgtatcga cccggaatgg gtagaacccg ttgctcagca tttgattaaa
     3601 cgcacctaca gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa
     3661 gtcactgttt atggtttgcc gattgttgct gcgcgcaagg tcaactacag ccagatcgat
     3721 tcggcgttat gtcgtgaact ctttattcgc cacgctctgg tggaaggtga ctggcagacg
     3781 cgtcacgcat tcttccgtga aaacctgaaa ctgcgggccg aagtggaaga gctggaacac
     3841 aaatcacgtc gccgcgatat tctggttgat gacgaaacgc tgtttgagtt ctacgaccag
     3901 cgcatcagcc acgatgtaat ctccgctcgt cacttcgata gctggtggaa aaaagtcagc
     3961 cgcgaaacgc ctgatttgct caactttgaa aagagcatgt tgatcaaaga aggggcggaa
     4021 aaaatcagca agctggatta cccgaacttc tggcatcagg gcaatctcaa gctgcgtttg
     4081 agctatcagt ttgagccagg cgcggacgcg gacggtgtga ccgtacatat tccgctgccg
     4141 ttacttaacc aggttgagga aagcgggttt gaatggcaga tccccggtct gcgccgcgaa
     4201 ttggtgattg ctctgattaa atcgttgccg aaaccggtac gccgtaattt tgtgcctgca
     4261 ccaaactacg ccgaagcgtt tttaggccgc gtcacaccgc tggagttacc gttgctcgac
     4321 agccttgagc gcgagttacg gcggatgacc ggcgttaccg ttgaccgcga agactggcac
     4381 tgggatcagg tgcccgatca cctgaaaatc accttccgcg tggtggatga caaaaacaag
     4441 aagctaaaag aagggcgctc gctacaagat ctgaaagatg cgctgaaagg caaagtgcag
     4501 gaaacgctgt ccgcggtggc ggatgacggt atcgagcaga gcggcttaca tatctggagt
     4561 tttggtcagc tgccggaaag ttacgaacag aagcgtggca actataaagt gaaggcgtgg
     4621 ccggcgctgg tggatgaacg cgacagcgtg gcgatcaaac tgtttgataa cccgctggag
     4681 caaaaacagg caatgtggaa tggtcttcgc cgtctactgc tgctaaatat ttcctcgccg
     4741 atcaagtatt tacatgagaa gttaccaaac aaagccaagc tggggctgta ctttaacccg
     4801 tatggcaaag tgctggagct gatcgacgac tgtatctcct gcggtgtgga tcaactgatc
     4861 gacgccaatg atggcccggt ctggacggaa gaaggctttg ctgcgctgca tgaaaaagtg
     4921 cgcgccgaac tgaatgacac ggtggtggat attgcgaagc aggtcgagca aatcctcact
     4981 gcggtgttca atatcaacaa acgtctgaaa gggcgggtgg atatgaccat ggcactgggg
     5041 ctttctgaca ttaaagcgca gatgggcggg ttggtatatc gcggttttgt cactggtaac
     5101 ggcttcaaac gtctgggcga cacgctgcga tatttgcagg cgattgaaaa acgactggaa
     5161 aaactggcgg ttgatccaca tcgcgatcgt gcacagatgc tgaaagtcga aaacgtccag
     5221 caggcgtggc agcaatggat caacaaactg ccgcctgcac gtcgtgagga tgaagacgtg
     5281 aaagagatcc gttggatgat agaagagctg cgcgttagtt acttcgctca acaacttggt
     5341 acgccttatc cgattt
//
LOCUS       DAJTYF010000194         5335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTYF010000194 DAJTYF010000000
VERSION     DAJTYF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.196"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO590_003958"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO590_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6639112.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO590_003959"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO590_003959"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6639113.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO590_003960"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO590_003960"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6639114.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO590_003961"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO590_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6639115.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1289 c         1454 g         1283 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaactc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgactttc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaaattaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcg
//
LOCUS       DAJTYF010000195         5333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTYF010000195 DAJTYF010000000
VERSION     DAJTYF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.197"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..789)
                     /locus_tag="OO590_003962"
     CDS             complement(<1..789)
                     /locus_tag="OO590_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR6639116.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLG"
     gene            1035..1661
                     /gene="gstB"
                     /locus_tag="OO590_003963"
                     /pseudo
     CDS             1035..1661
                     /gene="gstB"
                     /locus_tag="OO590_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(1658..2772)
                     /gene="yliI"
                     /locus_tag="OO590_003964"
                     /pseudo
     CDS             complement(1658..2772)
                     /gene="yliI"
                     /locus_tag="OO590_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2883..3266)
                     /gene="bssR"
                     /locus_tag="OO590_003965"
     CDS             complement(2883..3266)
                     /gene="bssR"
                     /locus_tag="OO590_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6639117.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3479..4804
                     /gene="rimO"
                     /locus_tag="OO590_003966"
     CDS             3479..4804
                     /gene="rimO"
                     /locus_tag="OO590_003966"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6639118.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4851..>5333)
                     /locus_tag="OO590_003967"
     CDS             complement(4851..>5333)
                     /locus_tag="OO590_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6639119.1"
                     /translation="ADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLF
                     DVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEE
                     LLSILKEIVHFQVGSINLSTSIGVAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ
                     "
BASE COUNT         1330 a         1382 c         1283 g         1338 t
ORIGIN      
        1 ccccagcact acggagatta aacgcatatc accctgggta gccgaagcaa ccagattata
       61 tcccgcacct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
      121 cagacggtta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
      181 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
      241 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      301 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      361 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      421 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      481 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      541 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      601 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      661 aatccatgca cgcgcatcca cgctcggcgc ttcaacggtt tgttcggccg cgaatgccgt
      721 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      781 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaat acgttatatc gccgtatgat
      841 aagttcggtg attaataacg cacacacaaa cttaaagaaa ccgtaagttg gtaaagtttt
      901 taaagtttat gcaatatcct gccgcttcgc tgcttctggt gtcatttttt tgccagctgt
      961 tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt cagtctgtgg ctgcctgtcg
     1021 gaaaaggggt gagtatgatt acgctgtggg gtcggaataa ttcaactaac gtaaaaaaag
     1081 tattgctgac gctcgaataa ctggaactac cttatgagca aattctcgcg ggccgtgagt
     1141 ttgggataaa tcacgatgct gattttctgg cgatgaaccc taacgggctg gtgccgttgt
     1201 tgcgtgacga cgaaagtgat ctcattcttt gggaatcaaa cgccattgtc cgctatctgg
     1261 cggcacagta cgggcaaaaa cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg
     1321 aaaaatggat ggactgggca aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg
     1381 gattagtcag aacaccaccg gaagagcgcg atcaggccgc cattgatgcc agttgcaaag
     1441 agtgcgacgc cctgtttgcc ctgctcgatg cggaactggc aaaagtaaaa tggttctctg
     1501 gcgacgagtt tggtgtgggc gatatcgcta tcgcaccgtt catctacaat ttgtttaacg
     1561 ttggcctgac ctggacaccg cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac
     1621 gccccgcagt gcgcaaagtg gtgatgattc ccgttagcta attgcgtggg ctaactttaa
     1681 gtaattcccc actggactcg tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa
     1741 catcacgaat tcgctgccct ctgtccgtta aaatacggcc atcttctgtc actttgtcgc
     1801 cgttgacgct catcacaatg acatctttat ctttcagcgc ccaataaata atttttgctg
     1861 ccactggggg aatttatcgc tgttatagaa ggccatgccg ctcacagcgg gcgaatcttt
     1921 ccagtaaaaa acaggttgct cggtcccggc gacgatctcc cctttcgctt ccggtatctt
     1981 aaagcctgaa tagttgattc cccaggttgc cagcggccag ccgtagtttt tgcctttttg
     2041 cgggatatta atttcatcac caccgcgcgg gccatgttca ttcagccaca gtgcattact
     2101 ccacggattc atcgccattc cttgcggatt acgaatgcca taagaccaga tctcgccgcg
     2161 cgcaccggat tcctttataa aaggattatc atccgggatt tcgccctggt cggtcagacg
     2221 caccagtttg ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc
     2281 cagagcaata aaaagataac ctttaccgtc gaataccagc cgcccgccaa aatggttgcc
     2341 ggtagacagt tttggcatct ggcggaagac ggtgcggaag tcggtcactt ttgagagatc
     2401 atcacttaag cggccataac ccacagcagt tccggctttg ccatcatcgc caacttcgga
     2461 ataacttaac cagatgcggc gagactgagc aaaatcaggc gctaaaacca cgtccagcag
     2521 gccgccctgc ccgtgcgccc aaacgtccgg aactccggaa agcggcgcag ataatccttt
     2581 tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg attaacatac cgtgattatc
     2641 gggtaaaaag gccagtgccc agggatggtc gagtttgtct tgcagtactt cgacatttac
     2701 cgttgcagga gccgcccaga gagcggaaga aagacaaata aggggcacaa ggaaaaagga
     2761 ttgtcgatgc ataacgcctc cggattgact ctggcttaaa gcgtagtcag tggaggagat
     2821 aacaaattca tttttacaaa aacttaaaca tgaaggggga gacgctttct cccccttagt
     2881 tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct ggatactcag cgttaagctc
     2941 atcagacaat tttcaagctt atcggcgttg acggtaataa cagtcgggca atcatggtgc
     3001 ccactcatca aacatactgc ggctgtcgct aatgcttctt cagcatgatg aagagcactc
     3061 cactcttcct gatccagatg aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc
     3121 agtttaaaaa agttatctcg tagatgattg ctttcgctga cggacatgta tccttttgcc
     3181 ttcctcagtt gcacgtatgc agcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga
     3241 tcgattcgct gtctgtcaac gaacatactt cgatcctcct ctttccagcc ttgttgcaat
     3301 taaatagtat ggctattttt tgagcaaaat gctgtctagg atcaattctt cacgccgaat
     3361 taacaaaatt tacatttcct tcgcctccgc cagcgaatga gattgcgata caaattttat
     3421 ccagaactct gtagaatccc tgccctgacc tttcctgtaa ctgaatattt tttaagctat
     3481 gagcaaagta actccccagc cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct
     3541 tgtcgattca gagcgtattc tcaccgaact ccgcactgaa ggttatgacg tggtaccgag
     3601 ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca
     3661 agaatcactg gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg
     3721 ttgtctgggg gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc tggaaatcac
     3781 cgggcctcat agctatgagc aggttctgga gcacgttcat cactacgtgc caaaaccgaa
     3841 acacaaccca ttcctgagcc tggtgccaga acaaggtgtg aaactgacgc cgcgtcatta
     3901 tgcctatctg aaaatttcgg aaggctgtaa tcaccgctgc accttctgca ttattccgtc
     3961 tatgcgcggc gacctggtga gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct
     4021 ggtagatgcg ggcgttaaag agattctggt gatctcgcag gatacttccg cctatggcgt
     4081 tgatgttaaa catcgtactg gcttccacaa cggcgagccg gtaaaaacca gcatggtcag
     4141 cctgtgcgaa cagttatcga aactggggat ctggacacgt ctgcactacg tttaccctta
     4201 tccgcatgtg gacgacgtca tcccactgat ggcagaaggc aaaatcctgc cgtatctgga
     4261 cattccgttg cagcacgcca gcccgcgcat tctcaaactg atgaagcgtc cgggttctgt
     4321 agatcgccaa ctggcgcgca tcaaacagtg gcgcgaaatc tgcccggaac tgaccctacg
     4381 ctcaaccttt attgtcggct tccctggcga gacggaagaa gatttccaga tgctactcga
     4441 cttcctgaaa gaagcacgtc tggatcgcgt tggctgcttt aaatacagcc cggttgaagg
     4501 tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa gtgaaagaag aacgctggaa
     4561 ccgtttcatg cagctgcagc agcagatttc cgccgagcgc ctgcaagaga aagtgggccg
     4621 tgaaattctg gtgattatcg acgaagtgga cgaagaaggc gcgattggtc gcagcatggc
     4681 agatgcaccg gaaatcgacg gcgcggttta tctcaatggt gaaaccaaca ttaagccggg
     4741 tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg gtagccgggt
     4801 ttaattaatt caggccgggc aaaatcattt gtccggcctg cgaataatga ttactgccag
     4861 ctaatctgtg ccctgccgtt tttcttactc ttatacagcc gctcatccgc cagcattttc
     4921 aagcgttcga cggtagtaca tttatttgaa tgcgctacac cgatactggt gcttaaatta
     4981 atgcttccca cctggaaatg aacgatttct tttagtatcg atagcaactc ttccagcgtg
     5041 ccgccagtat aaatcaccgc aaattcgtca cctccgaagc ggtagacttt acctttatct
     5101 accgggatct gtgatttaag gattttcacc actttcatca acacttcatc gcccagcaaa
     5161 tgaccgaagg tgtcgttaat gtttttgaat ttatcgacgt caaacagaca caaataaccg
     5221 cctttttccg gcagcgcttt taaatcttca tcaaaggatt ttctgcccat tgctttggtc
     5281 aggtcgtcca ggaacgccga gttatacata ctgtagagtt ttttgacatc ggc
//
LOCUS       DAJTYF010000196         5287 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTYF010000196 DAJTYF010000000
VERSION     DAJTYF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.198"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..586
                     /locus_tag="OO590_003968"
     CDS             <1..586
                     /locus_tag="OO590_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6639120.1"
                     /translation="TNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHM
                     YKIATLGSGNPRNFTCNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCS
                     SEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNK
                     SKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            920..3199
                     /gene="ydeP"
                     /locus_tag="OO590_003969"
     CDS             920..3199
                     /gene="ydeP"
                     /locus_tag="OO590_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6639121.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3447..3644
                     /gene="safA"
                     /locus_tag="OO590_003970"
     CDS             3447..3644
                     /gene="safA"
                     /locus_tag="OO590_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6639122.1"
                     /translation="MHATTVKNKIMQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3718..4479
                     /gene="ydeO"
                     /locus_tag="OO590_003971"
     CDS             3718..4479
                     /gene="ydeO"
                     /locus_tag="OO590_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6639123.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4881..>5287
                     /locus_tag="OO590_003972"
     CDS             4881..>5287
                     /locus_tag="OO590_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR6639124.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSR"
BASE COUNT         1528 a         1125 c         1170 g         1464 t
ORIGIN      
        1 gacaaatacc aacgtattgg atattggtga taagacaccg atgccattac cgttaaaact
       61 ctatattacc ccagttgggg cggccggtgg agttgtcata aaagcggggg aagtcattgc
      121 ccgtattcat atgtataaaa ttgcaacatt aggaagcgga aatccgcgta attttacgtg
      181 taatattatt tcaaataaca gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg
      241 taatgtcacc gtggatctcc cggactttcc tggaagcgca gaaatacccc ttggcgtata
      301 ttgctcaagc gaacaaaagc tctcatttta tctttcgggt gccaccactg acagtagccg
      361 acaagtgttt gctaacacag cacctgatgc aacaaaagca tcgggagtgg gtgtcactct
      421 aatgcgtaat ggaaaaatac tggctacggg agaaaatgtc tccctgggga cagtaaataa
      481 aagcaaagta cctctgggtt taagcgcaac ctatggacaa acaggaaaca aggtttcagc
      541 cggaaccgtc cagtcagtca ttggtgtcac ttttatctat gaataattgt gattttgacc
      601 ttagtctatg cgctccattc acgaaaattg cgcggataat tattttgtga aggctattat
      661 cctacacctg taagaaaatc cgcgcacaat tgaagccaga gtcttatttt ttatattgaa
      721 aaatatgtta atttaaacac tatgagagat gatgcttaac ttatcgtatt taatctattg
      781 tttaacgata atagattaaa aatagaaaga gagtagtccc ctactcttat atatccatgt
      841 tggcgataat ccctttgtat gtactgtgca tcatcgctat tacaaatcct aataattcat
      901 ttccacacag gataagtaga tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg
      961 ttggggtgct gttaaatccg tagcgaatgc agtacgtaag cagatggata tacgccatga
     1021 tgttattgcc atgtttgaca tgaataagcc agagggcttt gactgtccgg gttgtgcatg
     1081 gccagatcct aagcacagtg cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc
     1141 ctgggaagtc acggataagc aggtaaatgc ctctttcttt gctgagaata cggttcaatc
     1201 attacttacc tggggagatc acgagcttga ggctgcgggg cgtctcactc agcctttgaa
     1261 atatgatgcc gtcagcgact gttacaagcc attaagctgg caacaagctt tcgacgaaat
     1321 tggcgcacgc cttcaaagct atagtgatcc caatcaggtt gaattctata cttcgggccg
     1381 cacttccaat gaagctgcct ttctttatca gctttttgcc cgtgaatacg ggagcaataa
     1441 ctttcccgac tgctccaaca tgtgccatga accgacaagc gtgggtttgg cagcgagtat
     1501 cggtgtaggt aaagggaccg tgttgctgga agactttgag aagtgcgatt tagtcatttg
     1561 cattgggcat aaccctggta caaaccaccc tcgcatgctg acttcgttgc gcgctttagt
     1621 gaaacgggga gcgaaaatga tcgccatcaa tcctctacag gaacgtggcc tggagcgatt
     1681 taccgcaccg caaaacccgt ttgaaatgct gacgaactct gagactcagt tggccagtgc
     1741 ctactacaac gtgcgcattg gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt
     1801 aattgagcgc gatgatgctg caagtgccgc aggtcggccc tcattgcttg atgacgaatt
     1861 tattcaaacg cataccgtcg gctttgacga gctacgccgt gacgttctca attccgagtg
     1921 gaaagatatc gaacgtattt ctggactaag tcagacacaa atcgccgaac tggctgacgc
     1981 atatgccgct gccgaacgca ccattatctg ttacggaatg gggatcactc agcacgaaca
     2041 tggtacccag aacgtgcagc aactggtcaa tttgctgttg atgaaaggta acattggcaa
     2101 gcctggtgcg ggtatctgcc cactacgtgg tcactctaat gtacagggcg accgaaccgt
     2161 cggtatcacc gagaaaccgt ctgcagagtt tctggctcgc ctgggtgagc gctatggctt
     2221 ccccccacct catgcacctg gacatgctgc aattgccagc atgcaagcaa tatgtacggg
     2281 gcaggctcga gcattgatct gcatgggggg caactttgca ctggcaatgc cagatcggga
     2341 agcgagcgct gtaccgttaa cgcaattaga tttggcggta cacgtagcca ctaagcttaa
     2401 ccgctctcat ctgctgaccg cacggcatag ctatattctg ccggttctgg ggcgtagcga
     2461 gattgatatg caaaaaagcg gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat
     2521 tcatgcctcg cgtggcgtgt taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt
     2581 ggtcgcggga atcgcgcagg cagcactacc ccagagcgtg gtagcctggg agtatctggt
     2641 ggaagattat gatcgcattc gcaatgacat tgaagctgtg ctgccagagt tcgccgacta
     2701 taaccagcgc atccgtcatc ccggtggttt tcacctgata aatgcagctg ctgaaaggcg
     2761 ctggatgacg ccgtcaggta aggctaattt cattaccagc aaagggctgt tagaagatcc
     2821 ctcttcagcg tttaacagta agctggtcat ggcgacagta cgcagccacg atcagtacaa
     2881 cacgacgatt tatggtatgg atgatcgcta tcgaggggta ttcggtcaac gagatgtggt
     2941 ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct
     3001 tattgcgctt acgccagacg gtaagcgcag ctcacgccgc atggatagat taaaagtggt
     3061 catttaccct atggctgacc gctcactggt gacctatttt ccagaatcga atcacatgct
     3121 aacacttgat aaccacgatc cattaagtgg cattcctggc tataaaagta ttccggttga
     3181 attagaacca tcaaattaat gcctcttctc atttcttctg ctgtcatccg cacagcagaa
     3241 gaattcctca ttgactatta ttgcgcaatt tactcacatg gattaaatta aactacatac
     3301 tataagatat aaacttctgc ctacagctgt aagaaactcc gctcagtact gaagcaccag
     3361 tcctatttct tcttttctcc agcctgttat attaagcata ctgattaacg acttttaacg
     3421 ttatccgcta aataaacata tttgaaatgc atgcgaccac agtgaaaaac aaaatcatgc
     3481 aaagagataa ctataaagaa atcatgtctg caattgtggt tgtcttatta ctgacactta
     3541 cgttgatagc cattttttcg gcaattgatc agctgagtat ttcagaaatg ggtcgcattg
     3601 caagagatct tacacatttc attatcaata gtttgcaaga ctgaaaataa acagcaaatt
     3661 ataaatgtga aatgtttaaa aggtatcgat aaaactttat tgttttaagg agataaaatg
     3721 tcgctcgttt gttctgttat atttattcat catgctttca acgctaacat tttagataaa
     3781 gattacgcct tctctgacgg cgagatcctg atggtagata acgctgttcg tacacatttt
     3841 gaaccttatg agcggcattt taaagaaatc ggatttaatg aaaataccat taaaaaatat
     3901 ctacaatgca ctaacatcca gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct
     3961 tcaaatgtac cgactggatt gcttaatgaa atgattgctt atctcaactc ggaagaacgc
     4021 aatcatcata atttttcaga acttttgctt ttttcttgcc tgtctatttt tgccgcatgc
     4081 aaaggtttca ttacactatt aactaacggt gtgctatccg tttccgggaa agtgagaaat
     4141 attgtcaaca tgaagctggc gcacccatgg aagctgaaag atatttgtga ctgcctgtac
     4201 atcagtgaaa gcctgttgaa gaaaaaacta aagcaagagc aaacgacatt ctcacagatt
     4261 ctattagatg ccagaatgca gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat
     4321 aaaattgccg aacaatgtgg ttatgccagt acatcttatt ttatttatgc gttccgcaaa
     4381 catttcggca acagtccgaa gagagtttct aaggagtacc gttgtcaaag gcacacgggt
     4441 atgaatacgg gcaacacgat gagtgcttta gctatttgat tatttgctaa cgagtagtca
     4501 accacgcacg ctacgtaaga attatatggg gcagccattc cctgccccgc gttgttttta
     4561 ggcgatatat ttattgaaat aaataagtga catccatcac atatttatgc acttgcataa
     4621 cctgttgcat gattatttat aatctcaatt cggcattttg tcagtaaaat gcaataattt
     4681 attaaatatc aataaattag ttatttatcg gcgagaaatt acttaataga acagaaagta
     4741 atgtcaacgc tttatggact gttatttccc tttttttagc taaatctgct atctctttat
     4801 gtgactaact tcacttacat ccacttattt ctcttcgtaa aattacttcg gaattaagta
     4861 caataagaag aggaacattt atgaagtctg cattaaagaa aagtgtcgta agtacctcga
     4921 tatctttgat actggcatct ggtatggctg catttgctgc tcatgcggca gatgatgtaa
     4981 agctgaaagc aaccaaaaca aacgttgctt tctcagactt tacgccgact gaatacagta
     5041 ccaaaggaaa gccaaatatt atcgtactaa ccatggatga tcttggttat ggacaacttc
     5101 cttttgataa gggatctttt gacccaaaaa caatggaaaa tcgtgaagtt gtcgatacct
     5161 acaaaatagg gatagataaa gccattgaag ctgcacaaaa atcaacgccg acgctccttt
     5221 cattaatgga tgaaggcgta cgttttacta acggctatgt ggcacacggt gtttccggcc
     5281 cctcccg
//
LOCUS       DAJTYF010000197         5162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTYF010000197 DAJTYF010000000
VERSION     DAJTYF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.199"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..605)
                     /locus_tag="OO590_003973"
     CDS             complement(<1..605)
                     /locus_tag="OO590_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6639125.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRTKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYIALSLRDA"
     gene            complement(583..1824)
                     /locus_tag="OO590_003974"
     CDS             complement(583..1824)
                     /locus_tag="OO590_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6639126.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(1824..2006)
                     /locus_tag="OO590_003975"
     CDS             complement(1824..2006)
                     /locus_tag="OO590_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639127.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(2018..3775)
                     /locus_tag="OO590_003976"
     CDS             complement(2018..3775)
                     /locus_tag="OO590_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6639128.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSAGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(3775..4257)
                     /locus_tag="OO590_003977"
     CDS             complement(3775..4257)
                     /locus_tag="OO590_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6639129.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(4405..4755)
                     /locus_tag="OO590_003978"
     CDS             complement(4405..4755)
                     /locus_tag="OO590_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6639130.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4798..>5162)
                     /locus_tag="OO590_003979"
     CDS             complement(4798..>5162)
                     /locus_tag="OO590_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6639131.1"
                     /translation="EQRDKNARELKLANATITDMQQRQRDVNALDAKYTKELADAKAE
                     NETLRADVAAGRRRLHIKAVCQSVREATTASGVDNATSPRLADTAERDYFTLRERLMT
                     MQMQLEGAQEYIRTQCIK"
BASE COUNT         1135 a         1535 c         1240 g         1252 t
ORIGIN      
        1 tcagcgtcgc gcagggacag tgcgatatag cccccggcca tgaatgcttt ggcttgggta
       61 cgggagagtc cgacatcacg caggactctt tcgatttttt tctgttcggg gatttccccg
      121 cgggccagcg cgttcttgac gtcgctgatc cgcgcctcgt cgttagacgg aaacgtcacc
      181 agactgactt cccagaggtc gatttctttc agcagaaagg cttctttcgt ccggtcgtat
      241 tcccagtcct tcaggacgta cccaatagaa aggccggtta acgaaccggc cttcatgtgt
      301 gcatgtgcgc gttttgccag gggatcatca tcaatgagca accgccccct gacgtaaagc
      361 ccgacatcgt cttccttcat ttcggtgtaa acaccgatgg gctcatccat gcggtgctgc
      421 cagagcagcg caggtaacgc ttttctgtca ctccacgccc gcagggaagc ggcaaatgcc
      481 ccggacatca ccacatcatc gtggctgtcc tttacaccaa agacggagcc atacccttca
      541 aactcaccgg agtcactgac agatttcaga ctcagcggta catcaagacg ttgtttcgtc
      601 tgcattggcg ttatccttct gcttaccggc tttactgcca tcggagggtt tcgtggtcat
      661 gttcatcggt gtgagataga catccccacc gggacgcgga ttcatatctt ccaggtcgcg
      721 gcagtcattg ggagagtaaa tcccccagtt aatcccggtg gcgtaggctt caaaacggga
      781 tttcatatcc ccgcgcagta acgccccggc gttaaatttg gcgtaaaact tcccctgttt
      841 gctctccctg accagccctg tattgatccg ttgttcaata cgggtcagat acggcacaag
      901 ggaatagttg ataaatccga gccccagctc ttcaatattg ttgaaagtgg cgcgatcggt
      961 gttctgcacc atgtgcagcg gcacgcggaa aagacgacag atttcttcca actgaaactt
     1021 gcgggtttcc aggaactggc tgtcctcggc attcagtgcc accggcttcc agtccagccc
     1081 catttccaga atcatcggac ggtgcgcatt acccagccca agatgacgct cctcaaaatc
     1141 cctcttcatg cgctcataag catccggcgt gagcttttgt tccgtgcgca acacaccgga
     1201 tgtcaccgca ccattaccaa acaacctggc accgtgctcc tcggttgccg ctgccagtga
     1261 aatggcctcg cgcgcatatg caatgggatt cagcccgacc agtccgtcca gcgtcagggt
     1321 gcgcacatgc cagatttcat cctgggtcaa cacatccacg gaaccatccg gaaacgtcac
     1381 ctgataaacc ggctgccact ggctgttcag cttcggttcc acacagcccg gatctatcgg
     1441 aagaagctcc acaacttccc ccagcgcctt caccttgtaa gcgtaaaaat tcccccgcag
     1501 acacaggcag acgataacca gctcccagaa ttcctgcggc gtcatgtagc cattgggttt
     1561 tgctgaaatc agcttatgca gccgttcatc caccgcccgt gttttaaggg tgccgctgat
     1621 tttgtagaga ctgcagggca gcataccaac agactcagcc agcaccctga cgcaggaata
     1681 gaccgccgtc agccgcatgg cccgctggct gctgatccgc tttccggtat aggtgtcgta
     1741 tgacaacccc agctcttccg caagcatccc gggcgttgtg acgggggtgt tatttttgcg
     1801 ttgaaaaagc ccctggaaaa acattactca cctccggagg cgacccggtg accgcgatcg
     1861 agataacgcg caaccagcca cgaccagcac agacacagcg ccccggcaac aacaaaacct
     1921 gccgggggat aaatcagcca tgtgccatac gacagcaaaa gcgcccccag cacgcccacc
     1981 agtggcgtga gaattatcag aaacataatg acctcggtta aagcgagcga ataccaacgc
     2041 tgaccagatg ctcagacaga tccggctccg gttcaccgcc attgaccagc atccggctca
     2101 ttgctgtaaa catcgcaaca gggccgtcga ttttggcttc cggcgtggat ttgttcggga
     2161 agatattgtc gtttttgtcc ggtttcaccg taacgttaga catcatccag ttcatgaccg
     2221 ggtgattact gtgatggaaa cgcccggcat agaccagtga ttccgtttcc ttcatggcct
     2281 ctgacagatt gcggaccgtc tgcggaactt ccaccagcgg tataccttct tcagccagcg
     2341 ccagactgaa ctgcattgcg ctccacgggt caaatcccag ttccctcagg ttttcaccgc
     2401 caatccattc cagtaagtca ctttttatct gagcatgatc gataacatca ccatccgtca
     2461 gaatcagctt atccatctcc gcccacttcc ggtaaagttc tgcctgctgc cgcgagcatc
     2521 gttccagccg tccttccgga agccagaatt taaaatcggc atgaacatgc ccgttatccg
     2581 ttcgccagag ttttgccgcc gcacagatat caatcttatg agcaaggtca acgccgaccc
     2641 acatgggata cgttttcagc tcatgtcgtg gggcaatgta ttcgcacttc tcccacttaa
     2701 tcatgtccat ccaggcagac tctgctgtta cccacacatt catgtgtttg gtaaaaaaat
     2761 tcacccgcgc agagacctgt tctttcgctt ttttcgccag gcgacgcaga tcatcccagc
     2821 gtttacagat gcccaggccg ggattcgctt tctgccagac cgtttcatca aacggatcat
     2881 ctccctcatc gagggtgtaa ataatcgcaa agtaggagtc gtcttttacc gcgccctcca
     2941 cgtcgctgtt atagccacgc aataccttga tggcgtaatc acgctgctcg taacaaattc
     3001 cttccttgtt aaaccctgcc gtggtgatac caaataaaag ggactgcaga cgggcaccgg
     3061 ttgccgtttc cagaacgtcc cacacgtcac gggttttatg tgcatgcagc tcatcaataa
     3121 tggcgcagtg gatgttcaga ccatccaggt tgtttgcatc tgaagaaagc ggttcaaatt
     3181 ttgatgcgct ctgctcctga taaatcgcca gcttgttgaa atcaaacaac cgcccgagtg
     3241 tcgaccgggc ttttctgacc atatttttgg cgtcttcaaa cacgattctg gcctggtcac
     3301 gcgtggttgc ggctgaatac acctcagccc cgccttcacc atctgccccc gtcatataca
     3361 gaccgatacc tgctgacagg gttgattttg cgtttttacg ggcgacttcg ttgtacgctg
     3421 tccggaaacg gcgcaccatc accgggcgtc cgctgccatc gctgcgcatg acaacttccc
     3481 cggtctcttc attcaccagc ggaatgacaa aaccaaaaat attaatgagg ataaacacat
     3541 gccagtccat caactcaatg ggctgacctg ccagcgcccc ttttacatgg ggcacaaatt
     3601 tgtagaaatt caggatgtgc tgcgcacggg gttcactgaa ataaatcccc cgctcttcgc
     3661 cgtacttcag atcatcaaga aaacgctggc aggccagacg gacaaattca ccagcgacaa
     3721 tttctcctgc aacaacacgt tcggcgtagc ggatcccgtc agccactttt gccatcagtc
     3781 tctcgctttt aaaagttctg ccagcggatc aacatcatcc ggtccggcga tatttacttt
     3841 cgcccggctt gccggtgaca taccaaattc tgcaagcatc gcccggatcc gcttccaggc
     3901 atccgctttc atcgcagcag ccgggtgtgc cttaatcagc acatcaccgt tctgcgtttc
     3961 cgtgcggtag gtataaccct caacatcgag ggtttcgcag tgatgccggt attcggtgta
     4021 ggcttccacc agcaactcga gtgcacgcgc atcaagctga gaaatgatcc cttccgcatt
     4081 cagctcttcc gccattcgcc tgaaccagta ctttccctgc gcccctaaat gctgcggaat
     4141 tttagggaga cctttttcgt cctttttagc ggtttttttg gcgtctttaa cgggacgctt
     4201 tgagggattg cctcgtatca aatgcaggcg tggcggggtt ttcgggggtc ctgacataat
     4261 cgattttacc tatcaatcgc ttgatcgcat tcccaaaaaa aagttttcga acctgcggcg
     4321 atgcgaggaa gggttggcgg gcggtcccgg acagccaggg ctgcagggat ttgacccgcc
     4381 cctccctaca agtgagaata attatcacct gattcgttcg cgcgctgttt tcgctttatg
     4441 gcagggccag cacagactct gcaggttgct gtctgcgtct gttccgccat gcgctttcgg
     4501 gatgatgtgg tcgacggttt tcgcctcgct caccacaccg acacgcagac acaactgaca
     4561 cagaccttta tcgcgcttca gaatacgggc acgaatcacc gtccattttg agccatagcc
     4621 acgctggtgg cggctcagtc cgcgctggtg ctgcacccag ccttcaccgc gatgcttatc
     4681 gcagtaaccc gaactgtctg tggttgtacc tgcacatcca cgcttacggc aggcacgtgg
     4741 gattagtgat ggcataaata cctcataccc tgcgaaatgt ttaccacgat aaaaaggcta
     4801 cttaatgcac tgagtgcgga tatactcctg tgccccttcc agttgcatct gcatcgtcat
     4861 cagccgctct ctgagggtga aataatcccg ttcagcggtg tctgccagtc gggggctggt
     4921 tgcattatcc acgccggagg cggtggtggc ttcacgcact gactgacaga ctgctttgat
     4981 gtgcaaccga cgacgaccag cggcaacatc agcgcgcaga gtttcatttt cagctttcgc
     5041 atcagctaac tccttcgtgt atttagcatc gagtgcatta acatcacgct gtctctgctg
     5101 catgtcagta atggttgcgt tcgccagctt cagttctctt gcgtttttgt cgcgctgctc
     5161 tt
//
LOCUS       DAJTYF010000198         5074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTYF010000198 DAJTYF010000000
VERSION     DAJTYF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.200"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..609
                     /locus_tag="OO590_003980"
                     /pseudo
     CDS             1..609
                     /locus_tag="OO590_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            673..1599
                     /gene="glsB"
                     /locus_tag="OO590_003981"
     CDS             673..1599
                     /gene="glsB"
                     /locus_tag="OO590_003981"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6639132.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1566..1958
                     /locus_tag="OO590_003982"
     CDS             1566..1958
                     /locus_tag="OO590_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6639133.1"
                     /translation="MDETVRAFGLLRQSLDPLFARLSRSKFRSRFRLGMKERQYCLEK
                     GAPVIEQHAADFVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIP
                     QGVSLSEEQQRYIVAVIYHWLVVQMNQP"
     gene            2097..3515
                     /locus_tag="OO590_003983"
     CDS             2097..3515
                     /locus_tag="OO590_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6639134.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3742..>5074
                     /gene="uxaB"
                     /locus_tag="OO590_003984"
     CDS             3742..>5074
                     /gene="uxaB"
                     /locus_tag="OO590_003984"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6639135.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLA"
BASE COUNT         1166 a         1183 c         1374 g         1351 t
ORIGIN      
        1 ttcccatcat tcttgcaggt aacggctact tacttaaaca tgcgccgaat gtgatgggct
       61 gtgctcagct cattgcccag gtgtttaaag atgcgggaat cccgcaaggc gtatatggct
      121 ggctgaatgc cgacaacgac ggtgtcagtc agatgattaa agactcgcgc attgctgctg
      181 tcacggtgac cggaagtgtt cgtgctggag cggctattgg cgcacaggct ggagcggcac
      241 tgaaaaaatg cgtactacta tccgccaacg gttctggcga atgttacccc agaaatgacc
      301 gcgtttcggg aagaaatgtt tggccccgtt gctgcaatca ccattgcgaa agatgcagaa
      361 catgcgctgg aactggctaa tgatagtgag ttcggccttt cagcgaccat ttttaccacc
      421 gacgaaacac aggccagaca gatggcggca cgtctggaat gcggtggggt gtttatcaat
      481 ggttattgtg ccagcgacgc gtgagtggcc tttggtggcg tgaaaaagag tggctttggt
      541 cgtgagcttt cccatttcgg cttacacgaa ttctgtaata tccagacagt gtggaaagac
      601 cggatctgac gcgacatatt cagctctgat atactcgcag gtcttttcag acctgcggtc
      661 caggagtaga aagtggcagt cgccatggat aatgcaattt tagaaaacat cttgcggcaa
      721 gtgcggccgc tcattggtca gggtaaagtc gcggattata ttccggcgct ggctacagta
      781 gacggttccc gattggggat tgctatctgt accgttgacg gacagctttt tcaggccgga
      841 gacgcgcaag aacgtttttc cattcagtct atttccaaag tgctgagtct cgttgtcgcc
      901 atgcgtcatt actccgaaga ggaaatctgg caacgcgtcg gcaaagatcc gtctggatca
      961 ccgttcaatt ctttagtgca actggaaatg gaacagggta ttccgcgtaa tccgttcatt
     1021 aatgccggtg cgctggtggt ctgcgatatg ttgcaagggc gactaagcgc accacggcaa
     1081 cgtatgctgg aagtcgtgcg cggcttaagc ggtgtgtctg atatttcata cgatacggtg
     1141 gtagcgcgtt ccgaatttga acattccgcg cgaaatgcgg ctatcgcttg gctgatgaag
     1201 tcgtttggca atttccatca tgacgtgaca accgttctgc aaaactactt tcattactgc
     1261 gctctgaaaa tgagctgtgt agagctggcc cggacgtttg tctttctggc taatcagggg
     1321 aaagctattc atattgatga acctgtggtg acgccaatgc aggcgcggca aattaacgcg
     1381 ctgatggcga ccagtggtat gtaccagaac gcgggggagt ttgcctggcg ggtgggacta
     1441 ccggcgaaat ctggcgttgg tggcggtatt gtggcgattg ttccgcatga aatggccatc
     1501 gcagtctgga gtccggaact ggatgatgca ggtaactcgc ttgcgggtat cgccgtcctt
     1561 gaacaattga cgaaacagtt agggcgttcg gtttattaag gcagtctctc gatccactct
     1621 tcgcgcgttt atcccgttca aaatttcgct ctcgctttcg tctgggcatg aaagagcgtc
     1681 agtattgcct ggagaaaggc gcgccagtca tcgaacaaca tgcggcggat tttgtcgcta
     1741 aacgtcttgc tccggcatta ccggctaatg acggtaagca accccccatg cgcgggcatc
     1801 cggtgtttat cgctcagcac gccaccgcga cctgctgtcg cggttgtctg gctaagtggc
     1861 ataacattcc gcagggtgtt tcgttaagcg aagaacaaca acgttatatc gtggcggtaa
     1921 tctatcactg gctggttgta cagatgaatc agccatgaga attgcggaaa acgataaaat
     1981 agcattccca gggtaccttt atttgccgcc gctgttagcg aacatgctat cctttatgag
     2041 aatttactta tcacgttgag ataattgcca cccgcaattg tatggacaga taattaatgc
     2101 atgtacaccc catttcaact ttccgtttgt tccaggaagg tcatctgcta cgtaatagca
     2161 tcgctatttt tgcgctaacc acgctgttct attttattgg tgcagagtta cggctggttc
     2221 acgaactttc tcttttctgg ccgctgaatg gcgtaatggc gggggtgttt gcccgctatg
     2281 tctggcttaa tcgactgcat tactatgcga tcagttatgt ggcgatgctg gtttatgatg
     2341 ccataaccac cgaatgggga ctggtttcac tggttatcaa tttctccaat atgatgttta
     2401 ttgttaccgt cgccttactg gtcgtgcggg ataagcgtct ggggaaaaat aagtatgagc
     2461 cagtaagtgc cttacggcta tttaattact gtctgattgc cgcattatta tgcgctattg
     2521 tcggggcgat tggttcggtc agtattgata gtctggattt ctggcctttg cttgcggact
     2581 ggttcagtga gcaattctca acgggcgtgt tgatcgtgcc ttgtatgctg acgttggcta
     2641 ttcctggagt gctgccgcgc tttaaagcag aacagatgat gcccgccatc gcgcttattg
     2701 tgtcggttat tgcctcggta gtcattggcg gagcggggag tctggcgttc ccgctgcctg
     2761 cattaatctg gtgtgcagtg cgctatacgc cgcaggtaac atgtctgttg acctttgtca
     2821 ccggtgcggt ggaaatcgta ctggtggcaa attcggtgat tgatatctcg gtcggttcgc
     2881 cgttctccat tccggaaatg ttctccgcac gtctcggtat tgccacgatg gcgatatgcc
     2941 caattatggt ttcttttagc gtggcagcga tcaatttgct aatgaagcaa gttgcgctgc
     3001 gagccgactt tgattttctg actcaggttt actcacggtc cggtctttat gaggcgctga
     3061 aaagtccatc gctgaaacag acacaacatc tgacggtcat gctgcttgat atcgactatt
     3121 tcaaaagcat taacgataac tatggacatg aatgtggcga taaagtgtta agcgtgtttg
     3181 cccggcatat tcagaagatt gtcggtgata aggggctggt ggcgcgcatg ggcggcgaag
     3241 aatttgctgt tgcagtgccg tcggtgaatc ctgtcgatgg tctgctaatg gcggaaaaaa
     3301 tccgtaaagg cgttgaactg caaccattca cctggcaaca aaaaacgctt tatctcacgg
     3361 taagtattgg cgtcggtagt ggtcgcgcat cgtaccgaac gctgaccgat gattttaata
     3421 aattgatggt cgaagccgat acgtgtctgt atcgctcgaa gaaagatggg cgcaaccgta
     3481 ccagcactat gcgttacggt gaggaagttg tctgacatct ttattcattc cgcgcgattt
     3541 gaatgcaatg tgttctacca ctttatgatc caggaaaagg cgtatttcct cgattaaacc
     3601 atgatccgcg ccacactttt ctcataaaac tgctcattaa tcatcaggag ttgatcaaaa
     3661 caggggttcg gcgtattaaa gtttgctaac ctgtcgcacc aattcaaaca ggttgtatga
     3721 ctaatcagaa gggaacccat tgtgaaaaca ctaaatcgtc gcgattttcc cggtgcacag
     3781 tatccagaac gtatcattca gtttggtgaa ggtaacttcc tgcgcgcctt tgttgactgg
     3841 caaatcgatc tcctgaatga gcacaccgat ctgaattctg gcgtggtcgt tgttcgtccg
     3901 attgaaactt cattcccgcc gtcactgagc acgcaggatg gtctgtacac caccattatc
     3961 cgtggcctga atgagaaggg ggaagcggtc agcgacgcgc gtctgattcg ctcggttaat
     4021 cgtgaaatca gcgtctacag tgaatacgat gaattcctga aactggcgca caacccggaa
     4081 atgcgctttg ttttctctaa caccaccgaa gcgggtatca gctaccatgc gggcgataaa
     4141 ttcgatgacg cgccagcggt aagctatccg gcaaaactga cccgtctgct gttcgaacgc
     4201 tttagccatt tcaacggtgc gctggataaa ggttggatca tcattccgtg tgagttgatt
     4261 gactacaatg gcgacgcgtt gcgtgaactg gtgctgcgct atgcgcaaga gtgggcactg
     4321 ccagaagcat ttattcaatg gctggatcag gctaacagct tctgttctac gctggtggac
     4381 cgtatcgtta ccggttatcc gcgcgatgaa gtggctaaac tggaagaaga actgggttat
     4441 cacgatggtt tcctcgacac cgctgaacat ttttacctgt ttgtgattca ggggccgaaa
     4501 tccttagcga ctgagctgcg cctggacaaa tatccgctca acgtgctgat tgttgacgat
     4561 attaaaccgt ataaagaacg caaagtggca atcctcaacg gtgcgcacac cgcgctggtg
     4621 ccggtggcgt tccaggcagg gctggatacc gtaggtgaag cgatgaacga tgctgaaatt
     4681 tgcgcattcg ttgaaaaagc catctacgaa gaaattattc cggtactgga tttgcctcgt
     4741 gatgaactgg aatctttcgc cagtgcagtc accgggcgtt tccgtaaccc gtacattaag
     4801 catcagctgc tgtctatcgc gctcaacggt atgaccaagt tccgcacccg catcctgccg
     4861 cagctgctgg cagggcagaa ggcaaacggc acacttccgg cgcgccttac tttcgcatta
     4921 gcggcattga ttgcgttcta tcgtggtgag cgtaacggag aaacatatcc ggtacaggac
     4981 gatgcacact ggctggaacg ttatcagcaa ctctggagcc agcatcgtga ccgtgttatc
     5041 ggtacccagg aactggtagc gattgtgttg gcag
//
LOCUS       DAJTYF010000199         5023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTYF010000199 DAJTYF010000000
VERSION     DAJTYF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.201"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            94..1209
                     /gene="adrA"
                     /locus_tag="OO590_003985"
                     /gene_synonym="dgcC"
     CDS             94..1209
                     /gene="adrA"
                     /locus_tag="OO590_003985"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6639136.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1226..2035)
                     /gene="proC"
                     /locus_tag="OO590_003986"
     CDS             complement(1226..2035)
                     /gene="proC"
                     /locus_tag="OO590_003986"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6639137.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2155..2613
                     /locus_tag="OO590_003987"
     CDS             2155..2613
                     /locus_tag="OO590_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6639138.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2796..3320
                     /gene="aroL"
                     /locus_tag="OO590_003988"
     CDS             2796..3320
                     /gene="aroL"
                     /locus_tag="OO590_003988"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6639139.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3370..3561
                     /gene="yaiA"
                     /locus_tag="OO590_003989"
     CDS             3370..3561
                     /gene="yaiA"
                     /locus_tag="OO590_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6639140.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3819..4496
                     /locus_tag="OO590_003990"
     CDS             3819..4496
                     /locus_tag="OO590_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6639141.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLEKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4568..4852
                     /gene="ppnP"
                     /locus_tag="OO590_003991"
     CDS             4568..4852
                     /gene="ppnP"
                     /locus_tag="OO590_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6639142.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1222 a         1193 c         1322 g         1286 t
ORIGIN      
        1 agtgctaacg ggtgagctac gaaagtggct cacccgaaat ttcatacttc tgcctttagc
       61 cccgtctcta taatttggga aaattgtttc tgagtgttcc caaaaataat gaatgatgaa
      121 aactttttca aaaaagcggc ggcgcatggg gaggaacctc ctttaactcc tcaaaacgaa
      181 catcagcggt ccgggctgcg cttcgcccgt cgcgtcagac taccccgtgc ggttggcctg
      241 gctggcatgt tcttaccgat tgcttcaacg ctggtttcgc acccgccgcc gggctggtgg
      301 tggctggtgt tggtcggctg ggcgttcgtc tggccgcatt tagcctggca gatagcgagc
      361 agggccgtcg atccgcttag ccgggaaatt tacaacttaa aaaccgatgc agtattagcg
      421 ggaatgtggg taggcgtaat gggcgtaaac gtgctgcctt ccaccgctat gttgatgatt
      481 ctgtgtctga atttgatggg ggcaggcggc ccccgtctgt ttgtcgcggg tctggtgttg
      541 atggtggttt cctgccttgt caccctcgag cttacgggca ttaccgtgtc gttcaatagt
      601 gcgccgctgg aatggtggct ctcccttccc attattgtcg tttatcctct gctgtttggc
      661 tgggtcagct accagacggc aaccaaactg gcggaacata aacgcaggtt gcaggtcatg
      721 agtactcgcg acggcatgac gggcgtgtat aaccgacgtc attgggaaac tatgttacgc
      781 aatgaatttg ataactgtcg gcggcataat cgcgatgcaa cgttactgat tatcgatatc
      841 gaccatttca agagcatcaa cgatacctgg ggtcatgatg tgggcgatga agcgattgtg
      901 gcgcttaccc gacagttaca aattaccctg cgcggtagcg atgtgattgg tcggtttggc
      961 ggcgatgagt ttgcagtaat catgtccggt acgccagctg agagcgccat taccgccatg
     1021 ttacgggtgc gtgaagggct aaacacgtta cgcttgccga atacgccaca ggtaacttta
     1081 cggattagtg tgggggttgc gccgctgaac ccacaaatga gtcactatcg tgagtggttg
     1141 aaatcggcag atttggcgct ttacaaagca aagaaagccg gacgtaaccg caccgaagtg
     1201 gcggcctgac gtccggcgaa agtcatcagg atttgctgag tttttctgat ttttccatac
     1261 acttcgtcat cgcttcgatc actgcggcac ggaagccttt ctcttccagt acgcgtaccg
     1321 cttcaatggt ggtacctccc ggtgagcaga ccatatcttt cagtgccccc ggatgttctc
     1381 ccgtttccag caccattttt gcggaaccca ttaccgcctg agcggcaaat ttatacgcct
     1441 ggacgcgtgg catcccgccc agcacggcgg cgtcggccat cgcttcgata aacataaata
     1501 cgtaggctgg cgaagaaccg ctcacaccga ccaccgggtg gatcatcggc tcagcaatta
     1561 cttccgcttc gccaaagcag cggaaaatat tcagcacatc agcggtatct tctggggtta
     1621 ccagcgcgtt tggcgttacg gaggtcatcc cggcattaac cagcgcggga gtgttcggca
     1681 tggcgcggat aattttccgg tcatggccca gcgcgcgggc aagctggtcg agcgtgacac
     1741 ctgcagcaat agaaacgacc agagagtctt tattcaggct ggaggtgatt tcgctaagca
     1801 ctttaatcat gatgccaggt ttaacggcag caaaaatgat gtcggcgatt tgcgccactt
     1861 cttgcgccga ttctgcggcg ttgatgccga actggtcatg cagggcggcg actttatccg
     1921 gggagggggt gtatacccag atttgccctg gaagcacctg accgctggca attagaccgc
     1981 cgagaatggc ttttcccata ttgccgcagc caataaaacc gattttcttt tccattgcct
     2041 cactcctgcc gtgaaattca ttgttttgat aatcgctggc agaagcataa acagaactat
     2101 gccggaaggc aaaagcgcga cacaatagag gattacccaa caaaggatga ctttatgaca
     2161 atttgggtgg atgccgacgc gtgtcccaat gtaattaaag agattttgta tcgcgcggcg
     2221 gaacgtatgc agatgccgct ggtactggta gcaaaccaga gtttacgcgt gccgccatcg
     2281 cgatttattc gtacgctgcg cgtcgcggca ggtttcgacg ttgccgataa cgaaattgtc
     2341 cggcagtgtg aagcgggcga tttggtgatc accgcagata tacctttggc tgctgaagcc
     2401 atcgagaaag gcgcagcggc gcttaatccg cgcggcgaac gttacacgcc agcgaccatt
     2461 cgtgagcgcc tgacgatgcg cgattttatg gataccttac gtgccagtgg gatccagacc
     2521 ggcggaccag atagcctttc acaacgtgac cgccaggcct ttgccgcgga gctggagaag
     2581 tggtggctgg aagtgcaacg tagtcgtggc taaatgtaat ttattattta cacttcattc
     2641 ttgaatattt attggtatag taagggatgt attgagattt tcactttaag tggaattttt
     2701 tctttacaat cgaaattgta ctagtttgat ggtatgatcg ctattctcat gacaccggct
     2761 ttcgccgcat tgcgacctat tggggaaaac ccacgatgac acaacctctt tttctgatcg
     2821 ggcctcgggg ctgtggtaaa acaacggtcg gaatggccct tgccgattcg cttaaccgtc
     2881 ggtttgtcga taccgatcag tggttgcaat cacagctcaa tatgacggtc gcggagatcg
     2941 tcgaaaggga agagtgggcg ggatttcgcg ccagagaaac ggcggcgctg gaagcggtaa
     3001 ctgcgccatc caccgttatc gctacaggcg gcggcattat tctgacggaa tttaatcgtc
     3061 acttcatgca aaataacggg atcgtggttt atttgtgtgc gccagtatca gtcctggtta
     3121 accgactgca agctgcaccg gaagaagatt tacggccaac cttaacggga aaaccgctga
     3181 gcgaagaagt tcaggaagtg ctggaagaac gcgatgcgct atatcgcgaa gttgcgcata
     3241 ttatcatcga cgcaacaaac gaacccaacc aggtgatttc tgaaattcgt agcgccctgg
     3301 cacagacgat caattgttga ttttagagcg cctatactta acgttcatcc cgtgaaataa
     3361 ggaagaacga tgccaacgaa accgccttat cctcgtgaag catatatagt gacgattgaa
     3421 aaaggaaagc caggacagac ggtaacctgg taccaactca gagccgatca tcctaaacca
     3481 gactcgttga tcagtgaaca tccgacggct caggaagcga tggatgcgaa aaaacgctat
     3541 gaggaccctg acaaagagtg accgcatcag actgctcgga agggattctg agtgccacta
     3601 caagggatct gcgtcacatt tttcataatt catgtttttc taataattag aatattaaac
     3661 aataacattc cattactgga atcatttgga atctttacat tatgccgtgc acgtctgctg
     3721 ctacgctttt tgtcatttgt agcacaagta agtgtcagca gtggtgcttc acacttgccc
     3781 ggtaattaac gacgaaagaa aagtaaggtg gatgaacaat gagtgcgtcg ttggcgatcc
     3841 tgaccatcgg cattgtacct atgcaggagg ttttgccgct cctgacggaa tacattgacg
     3901 aagataatat ttcccatcat agcctgctgg agaagttaag tcgtgaagaa gtgatggcgg
     3961 agtacgcgcc agaagcaggc gaagacacca ttctcacatt attaaatgac aaccagctgg
     4021 cccatgtttc gcgtcgtaaa gtggagcgag atctgcaagg tgtggttgaa gtgctcgata
     4081 atcagggtta tgacgtcatt ttattaatga gtacagcaaa cattagtagt atgactgcgc
     4141 gtaatacgat ctttcttgag ccgtcgcgaa tattgcctcc actggtttcc tctattgttg
     4201 aagatcatca ggtgggggtt atcgttccgg ttgaggagat gctgcccgtt caggcgcaaa
     4261 aatggcaaat tttgcagaaa tcgccggtat tttcattggg taaccccatt catgattcag
     4321 aacaaaaaat cattgatgct gggaaagaat tactggcaaa aggggctgat gtcatcatgc
     4381 tggattgttt aggattccac caacgtcatc gcgatttact gcaaaaacag ctcgatgttc
     4441 ctgtcttgct ctctaacgta ttgattgcac ggctggctgc ggaattactg gtgtaatttt
     4501 gcgtgacagc cagcgcctct ggcccctata gtgaagtaga tgttcaacta ccagacaggg
     4561 ccagtttatg cttcaaagta atgagtactt ttccggcaaa gtgaaatcga tcggcttttc
     4621 cagcagcagc actggtcgcg ccagcgtggg tgttatggtt gaaggcgaat acaccttcag
     4681 caccgctgag ccggaagaga tgacggtaat cagtggcgcg ctgaatgtgt tactgcctga
     4741 cgcgaccgac tggcaagtgt atgaagccgg ttcggtgttt aatgttcccg gtcacagtga
     4801 gtttcatctt caagttgccg aacctacctc ttatctgtgc cgctatctgt aattcctcgc
     4861 cttccccttg aacgggaggg catttttctg aaatatcctc tctttaaccc ataataatat
     4921 ttcctttgct gcgatttttt caatttccga tatattcata attcatcaag gttgatataa
     4981 atatcagtga agatctccag ggtaatgact ccaacttact gat
//
LOCUS       DAJTYF010000200         4871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTYF010000200 DAJTYF010000000
VERSION     DAJTYF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.203"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..1101)
                     /gene="ycjM"
                     /locus_tag="OO590_003992"
     CDS             complement(<1..1101)
                     /gene="ycjM"
                     /locus_tag="OO590_003992"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR6639143.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="OO590_003993"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="OO590_003993"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6639144.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="OO590_003994"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="OO590_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6639145.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="OO590_003995"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="OO590_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6639146.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="OO590_003996"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="OO590_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6639147.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="OO590_003997"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="OO590_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6639148.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="OO590_003998"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="OO590_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6639149.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCATLNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..>4871)
                     /locus_tag="OO590_003999"
     CDS             complement(4502..>4871)
                     /locus_tag="OO590_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6639150.1"
                     /translation="NPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTLIDAKESVPA
                     IAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPL
                     TIPDAQFDAAMKILQDALSD"
BASE COUNT         1204 a         1231 c         1159 g         1277 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaatgat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggacgggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccaggta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gatctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga actgcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaagaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataattttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cgacgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctaataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt c
//
LOCUS       DAJTYF010000201         4754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTYF010000201 DAJTYF010000000
VERSION     DAJTYF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.204"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            278..808
                     /locus_tag="OO590_004000"
     CDS             278..808
                     /locus_tag="OO590_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6639151.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELISN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWCQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            815..1048
                     /locus_tag="OO590_004001"
     CDS             815..1048
                     /locus_tag="OO590_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6639152.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1628..2746)
                     /locus_tag="OO590_004002"
     CDS             complement(1628..2746)
                     /locus_tag="OO590_004002"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6639153.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSNKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2864..2953)
                     /locus_tag="OO590_004003"
     CDS             complement(2864..2953)
                     /locus_tag="OO590_004003"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639154.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(3389..3550)
                     /locus_tag="OO590_004004"
     CDS             complement(3389..3550)
                     /locus_tag="OO590_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6639155.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
     gene            complement(4202..4393)
                     /locus_tag="OO590_004005"
     CDS             complement(4202..4393)
                     /locus_tag="OO590_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6639156.1"
                     /translation="MTKQEKTALNMARFIRRQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            4457..4687
                     /locus_tag="OO590_004006"
     CDS             4457..4687
                     /locus_tag="OO590_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCR6639157.1"
                     /translation="MLTIRVTDDEHVRLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGDELRRLRLAVREQGTRDDS"
BASE COUNT         1327 a          985 c         1199 g         1243 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcaggacgct gacactccct gataatctgc cggaaacgaa acaggcgatt gctgagggcg
      121 ttacacgcgg tttaccatgc cggagagata aaaggccgtc aggatgtgat tcaggcgctc
      181 acagaggcag ggattgacgt ggtcagggtg acgcgaagca gtatcagcat tgcagacccg
      241 aacggcggga agaatgtcag actgaaagga gcgttttatg agcaatcttt tacagacggg
      301 tgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa tgctgaacga
      361 cgagttcagg aagctcgaag aatctgtaaa cagggaactg atctcaaacg aatctttaat
      421 caggaacgct atcaacgacc atacatcagc cctgaaagaa cttctggaga ggtatcagaa
      481 aacgaccgtc gatacgatgg acgcacactg gaaaaccgta ctgaaaatga gcgtgaaacg
      541 ctggctgtgg ctgattatcg tctcagtact gatgtttgcc acgaccggga gcctgctgtg
      601 gtgccagggc atgaagataa acgcgaacat gaatattctc cgcgagcaga aagagagtct
      661 ggagaagctg aacgcgaaga catggggcgt gcgttatcac gaggacagca acggacgttt
      721 tctggtgttg cccgagggga tgaaagccga aacgaactgg acgatggaaa acgggaaaca
      781 gaatgccgta aggctggtac aggagtaaca gagcatgacg gagctggaaa gacatttgct
      841 gaacgcatta gagcaactgc aacaggacta tatgcagcgg ctgaacgaat gggagagcgc
      901 cttcgtggaa ttgcagaaaa tgttttcgct tacgcaacgg gacaacgcga tgctgaacga
      961 gcgggtcatg cagttgagtc agcaggtgca gcacttgagc gagcagacag aacgcttgag
     1021 ccagttatac agcgagaaca ggagataagg gacgaacggc tgatacagga atgcgaacat
     1081 gcgttatccc tggagcgtga gcgacagccg gaaatacagg aacggacact ggatggccct
     1141 tcgctgggat ggtaatcaga gacaggctaa aatgttgtca gcgcgtttcc ggtgcatttt
     1201 ttgggataat ggttggtttg gggggcaccg ggaacgcgct tcagtcagac cttgctgaaa
     1261 acataggaac gcgttaaaac gcatacgggc gcgattctgg tgtgtctttt gttgctgtgt
     1321 tttgcctcta ttgccagatc gcgctcattc ccctgaactt ctgttttccc gctcaggtcc
     1381 ccgtcttctt cgcttccgaa agcatgaaaa aaatccgttt cagctgtctc tggctggcaa
     1441 ttcctgcgtg attcacatgg ctgcatagct atgcatgtgg ttaaaattta ccgggtgcga
     1501 tcgcggcagt ttttcgggtg gtttgttgcc atttttacct gtctgctgcc gtgatcgcgc
     1561 cgaacgcgtt tcagcggtgc gtacaatgag atgttatggt aaattcaagt cttagatttt
     1621 atgatgttta attagctttt tttgcgttta taatgttcat cttttttatt aaaattgatt
     1681 caatctttga tgtgggtatt aatattttat ttgacaaata gaaagttaaa aaaaacacca
     1741 ttaatagtat aaataaatac ccaaaaacag aagcgttatt aatttcaatt attgatgtta
     1801 atctgtatat tactacatgc tgaatcaaca tggatatgta gatgcattta gcatatgagt
     1861 tgaaaaaata gaggttaaat ttcttattaa ttattaatgc tgctttgtaa atgataatgc
     1921 ttatgcacat taaaggtatt attgataact ggtgattgac tatgtttgtt ttgaactcat
     1981 taggccctat taataatact gtcaatatac atataacaac cgatagaata actgtggtgt
     2041 ttttttgtaa tttatttttg tatttaaatg caagcatccc aaccataaac tcaggaagtc
     2101 ggcaagcaaa aaaccaaaat gggaagttta aaaatgatga tgagatattt gatatcggga
     2161 atgttgtaat tgttatcaca aataaaaaaa atatgttacg tttaaatgta aaggtattca
     2221 taacgtgaaa aactatcggt gatatcacgt acagcataat aacgcaccca ataaaccact
     2281 cgcctgtaag gaaaagaaaa tcataagaga aatgggcgtt taatatacca tcaagggcaa
     2341 aaaatactgg aattattcta gtgtactggc ctcctgttgt cattattgat acgagaggga
     2401 ctgtattaat tatctcttta tttaaataag acaataaaat taagagtaaa gagataaaaa
     2461 agtaagtgat aatataagga gtaaggatcc ttactccttt gtcaaggaaa taatcaatgg
     2521 tattctttct atctagtgaa ataaatgcca aatatccaga tataaaaaaa aacaagctca
     2581 cacctacctg gccaagaacc cctccataaa atgatggtat gaaagatagc ctggtttggc
     2641 taaatatgta gccataatga gatataataa caaaaatgca ggctgttact cgcaatgtat
     2701 caattgacgg atctctatta gacataacct ttatttcgct gttcaaatta tcgcctttag
     2761 ttgatgatgt aaaattaacg ctataaataa gctacacatt ctagagcagc gaaaggatga
     2821 ttcaagtatt tttaataata aaacgcattg ataatgttta tgttcagatg gtgctgatag
     2881 ctttgtcatg ctgttccact gatgttgata acgacagaga cttttgtcct tttcttgtta
     2941 gatgctgcct cagattcgga ttatggcgtt aaaggggttt gacgctcaac cgaacggttt
     3001 cacacgttaa gagctttttg tctgataaat caaaatataa tggacattga aaaacgccct
     3061 caaaacggtg tctttttaat cgaagattgg cacaaaacgg acaccaccgt ccattagtcg
     3121 attttttaga cggcttttct cgcctaacgt gagttttcgt tcggttgagc atcagacccc
     3181 ttttatattg tagatttcta tttttagaca gttggaacag gacaattaac aacagatcta
     3241 cttgagatca tttgcttgtc gtcgtagtct ctttcccaga aacgaaaaaa ccgccctgca
     3301 aggcggtttc tcgaaggttc tcagagcgac caactctttg aaccaaggta actggcttat
     3361 gaggagcgca gtaaccaaat ctgtcctttc agtttagcct taacaggcgc ataacttcaa
     3421 gactaactcc tctaaatccg ttaccaatgg ctaccgccac tggcgatttg tcgtgtcttt
     3481 ccgggttgga ctcaagttga tagttaccgg aacgggcgtg gtagtcggac tgaacggggg
     3541 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
     3601 gaatgagata aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
     3661 ggagagcgca cgagggagcc accaggggga aacgcctggt atctttatag tcctgtcggg
     3721 tttcgccacc tctgacttga gcgtcggttt ttgtgatgct cgtcaggggg gcggagccta
     3781 tggaaaaacg ccatcggcgc ggccttcttc ctgtttctgg tcttttgctc acatgttctt
     3841 tccggcgtta tcccctgatt ctgtggataa cagcatttcg tttttgagtg agtagatacc
     3901 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc
     3961 ctggacgtgc attttctcct tacgcatctg tgcggcattt cacacccggt atggcgcact
     4021 tttcatacaa tctgcactga tgccgcatgg ttaagcccgt atacactccg ctatcgctac
     4081 gtgactgggt catggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg
     4141 cttgtctgct cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatat
     4201 gtcagaggtt ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg
     4261 cgtggtcgtg aagcgattca cagatatcag cctgttcatc cgcgtccagt tcgttgagct
     4321 tctccagcag cgttaatgtc tgccttctga taaagcgggc catgttaagg gcggtttttt
     4381 cctgtttagt cactgatacc tccgtgtaag ggggatttct gttcatgagg taatgatacc
     4441 gatgaaacgc gagaggatgc tcacgatacg ggttactgat gatgaacatg tccggttact
     4501 ggaacgctgt gagggtaaac agctggcggt atggatgcgg cgggtctgcc tgggtgagcc
     4561 ggttgcccgt tccgggaaac tgccgacact ggcaccgccg ttactgcgtc agctggctgc
     4621 catcggggac gagctgcgcc ggctgcgtct ggccgtcagg gaacagggga cgcgggatga
     4681 tagttaagtt tcatgcccgg ggaaaaggtg gtggcagtgg tccggttgat tacctgctgg
     4741 gaagggagcg taac
//
LOCUS       DAJTYF010000202         4724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTYF010000202 DAJTYF010000000
VERSION     DAJTYF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.205"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..236
                     /locus_tag="OO590_004007"
     CDS             <1..236
                     /locus_tag="OO590_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639158.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            276..816
                     /locus_tag="OO590_004008"
                     /pseudo
     CDS             276..816
                     /locus_tag="OO590_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            883..2301
                     /gene="dcm"
                     /locus_tag="OO590_004009"
     CDS             883..2301
                     /gene="dcm"
                     /locus_tag="OO590_004009"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6639159.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2282..2752
                     /gene="vsr"
                     /locus_tag="OO590_004010"
     CDS             2282..2752
                     /gene="vsr"
                     /locus_tag="OO590_004010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6639160.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="OO590_004011"
     CDS             complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="OO590_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6639161.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3834..>4724
                     /locus_tag="OO590_004012"
     CDS             3834..>4724
                     /locus_tag="OO590_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6639162.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGTIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAV"
BASE COUNT         1148 a         1148 c         1347 g         1081 t
ORIGIN      
        1 gggctttcta tacatggtgt ggctatttcg tctgggcaat ggcgcgatac atctgggtga
       61 tgagccggat ccccgatgcc cccgtgtcgg gatttgaaag tgatttagga tctaccgcgg
      121 ggaagtggtt aggagcattg gtcggatttc tgtttatggc cctggttggt gccttgttag
      181 gcggcattgc atggtacacc cgacctcgtc ccgcacgttg cagacgctat gaataaaaac
      241 tgctaaggtt gcgactcttt ggcaacggag cgcgtatgga gttacaacac tggcaggcac
      301 agttcgagaa ctggttgaaa aaccatcatc aacatcagga cgcggcgcat gacctgtgtc
      361 attttcgccg tgtctgggct acggcacaga aactggcggc agatgacgat gtcgatatgc
      421 tggtgatttt aaccgcctgt tattttcacg atatcgtgag cctagcgaaa aatcatccgc
      481 aacggcagcg ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt
      541 tcgtgcaatt tccggcggag aaaattgagg ccgtttgtca tgccattgcc gctcacagtt
      601 tcagcgcgca aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc
      661 tggaagccct gggggcgatt ggcctggcgc gcgtatttgc cgtttcaggg gcattgagct
      721 tggcgctgtt tgatggcgaa gctccgtgcc gaactggcgg gggagaatga aggtatcgat
      781 cacaaggtga tagatgcgtt ttcacccgct ggctgagcgc gtggccctaa acggcagtaa
      841 ttatgttaac ctgtcggcca tctcagatgg ccggttaaat ctatgcagga aaatatatca
      901 gtaaccgatt catacagcac cgggaatgcc gcacaggcaa tgctggagaa actgctgcaa
      961 atttatgatg ttaaaatgct ggtggcgcag cttaatggtg tgggtgagaa tcactggagc
     1021 gcggcaattt taaaacgtgc gctggcgaat gactcggcat ggcaccgttt aagtgagaaa
     1081 gagtttgccc atctgcaaac gttgttaccc aaaccaccgg aacatcatcc gcattatgcg
     1141 tttcgcttta tcgatctatt tgccggaatt ggcggcatcc gtcgtggttt tgaatcgatt
     1201 ggtggacagt gcgtgtttac cagcgaatgg aacaaacatg cggtacgcac ttataaagcc
     1261 aaccattatt gcgacccggc gacgcatcat tttaatgaag atatccgcga catcaccctc
     1321 agtcatcaag aaggcgtgag tgatgaggcg gcggcggaac atattcgtca acacattcct
     1381 gaacacgatg ttttactggc cggtttccct tgtcagccat tttcgctggc tggcgtatcg
     1441 aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg atacccaggg cacgctgttc
     1501 tttgatgtgg tgcgcattat cgacgcgcgt cgtccggcga tgtttgtgct cgaaaacgtc
     1561 aaaaacctga agagtcacga taagggaaaa acgttccgca tcatcatgca gacgctggac
     1621 gaactgggct atgacgttgc cgatgcagaa gataacgggc cggacgatcc gaaaatcatc
     1681 gatggtaaac atttcctgcc gcagcaccgt gaacgcatcg tgctggtggg ttttcgtcgc
     1741 gatctgaatc tgaaagccga ttttactctg cgtgatatca gcgaatgttt ccctgcacag
     1801 cgagtgacgc tggcgcagct gctggacccg atggttgagg cgaaatatat cctgacgccg
     1861 gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc aggcgcgcgg taacggcttc
     1921 ggttatggaa tggtttatcc gaacaatccg caaagcgtca cccgtacgct gtctgctcgt
     1981 tattacaaag atggcgcgga aattttaatc gatcgcggtt gggatatggc cacgggtgag
     2041 aaagactttg acgatccgct gaatcagcaa catcgtccac gtcggttaac gcctcgtgaa
     2101 tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga aattccgcat tccggtttcg
     2161 gacactcagg cctatcgcca gttcggcaac tcggtggtcg tgccggtctt tgccgcggtg
     2221 gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt tgcgtcagca agaggcacaa
     2281 catggtcgac gttcacgata aggccacccg cagcaaaaat atgcgcgcga ttgccacgcg
     2341 tgatacggcg attgagaagc gcctcgccag tttgttaacc gggcagggcc ttgcgtttcg
     2401 cgttcaggac gccagtctgc ccggacgccc ggattttgtc gttgatgaat atcgctgcgt
     2461 gatatttacc cacggctgct tctggcatca tcatcactgc tatctgttta aagtgcctgc
     2521 gactcgaact gagttctggc tggagaagat aggtaaaaat gttgaacgcg atcgccgcga
     2581 tatcagtcgc ttgcaggagc tcggctggcg cgtactgatt gtctgggagt gtgcgttacg
     2641 taggcgcgag aagctgacgg atgcggcgct taccgagcgt ctggaagagt ggatctgcgg
     2701 cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat ttactcgctt gatgcatcct
     2761 gaataactgg cgcgacaacg ggtttggccg ggaagagata ttttcccaac gtgaccagta
     2821 ccaccgcgaa aacaattacg ccgagcgcca gccattcaat cttcgacagc gtttcgccac
     2881 ccagtcccgt acccagcaag accgcgacca ccgggttaac gtaggcgtag ctggtggcga
     2941 gagccggact gacattacgg attaaataca tataagcgtt aatggcgata atcgaaccaa
     3001 acagcgccag atagccgacc gcaaggaagc ctgacaggga agggagcgcc gtcagttttt
     3061 cacccgcaat catcgatgcg atcattaaca ccacgcctgc cgccagcatc tcaatcgcac
     3121 ccgccatcat ccctacaggt aaggtaatgc gcgagccata aactgagcca aacgcccagc
     3181 taatcgagcc gattaaaatc agaatcgcgc cccacggatt gccgcttaaa tttccaccgc
     3241 tattgagcat gatgattccg gcaagcccaa tggcaatacc cacccattcc agtttgcgcg
     3301 ttttaatgcc aaacaggcga ctgaagcaca gggtaaagag gggcacggtt gcaatcacta
     3361 cggcggcgat gccggaagga acattttgat gttcggcaac cgtcaccatg ccattaccga
     3421 cagccagcaa taacaggcca atcagcgcgg cattgagcag cggacgtagc gggggaagtt
     3481 tgtgtccgcg cagtagcaaa aatgccagca ataaaatacc ggctgccagg aatcgaacgc
     3541 ccgccatcat taacggaggc cagcttccca cgccaatccg aatgacaaaa taggttgagc
     3601 cccaaatgat atacaacgca aacagcgcgc caaaaagcgg taacaactgg cggaaacgca
     3661 taatccctca cggcggaaat aaaaaggtgg ttcatagtaa acgtgaaaat cattctgctg
     3721 gctagagata taattgcact tgattgttaa ataaatgttg acctgtgaag caagtatcag
     3781 agctgccgtt tttgcttcat acttacaccc ttcagcaata aaaatgagag ggaatgcttt
     3841 tggccgggag tagtttactg acgttgctcg atgatatcgc cacactgctg gacgatatct
     3901 ctgtgatggg caaactggcg gcgaagaaaa ccgccggtgt attaggggat gatttatcgc
     3961 tcaatgcgca acaagtttca ggcgtgcggg ccaaccggga acttcccgtg gtctggggcg
     4021 tggcgaaagg atcgctgatc aataaagtga ttctggtgcc gctggcgctg atcatcagtg
     4081 cgtttatccc gtgggcgatt acgccactgt tgatgattgg tggcgcgttt ctctgctttg
     4141 aaggggtaga gaaagtgctg catatgctgg aggcgcgtaa acataaagaa gatccagcgc
     4201 agagccagca gcgtctggag aagctggcgg cgcaggatcc gctgaagttt gaaaaggaca
     4261 aaattaaagg gacgattcgt accgatttta ttttgtctgc ggaaatcgtc gccatcacgc
     4321 tggggattgt ggcagaggcg ccgttgctta atcaggtgct ggtgctttca ggcatcgcgc
     4381 tggtagtgac cgtgggcgtc tacggtctgg taggggttat cgttaagatt gatgacctag
     4441 ggtattggct ggcggaaaaa tccagcgcgc tgatgcaggc attaggtaaa gggttattga
     4501 ttatcgcgcc ttggctgatg aaagcattat cgattgtcgg cacgctggcg atgttcctcg
     4561 tcggcggcgg gattgtggta catggtattg cgccgctgca tcacgctatt gaacatatcg
     4621 ccgggcagca aagtgcagtg gtggcgatga tattaccgac tgttttaaat ctgattcttg
     4681 gatttatcat cggcggcatc gtggtgctgg gagtgaaagc agta
//
LOCUS       DAJTYF010000203         4722 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTYF010000203 DAJTYF010000000
VERSION     DAJTYF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.206"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..1812)
                     /gene="recQ"
                     /locus_tag="OO590_004013"
     CDS             complement(<1..1812)
                     /gene="recQ"
                     /locus_tag="OO590_004013"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6639163.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLLDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVD"
     gene            complement(1945..2814)
                     /gene="pldA"
                     /locus_tag="OO590_004014"
     CDS             complement(1945..2814)
                     /gene="pldA"
                     /locus_tag="OO590_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6639164.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2979..3446
                     /locus_tag="OO590_004015"
     CDS             2979..3446
                     /locus_tag="OO590_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6639165.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3498..4406
                     /gene="rarD"
                     /locus_tag="OO590_004016"
     CDS             3498..4406
                     /gene="rarD"
                     /locus_tag="OO590_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6639166.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSKSKINIF"
     gene            4447..>4722
                     /locus_tag="OO590_004017"
     CDS             4447..>4722
                     /locus_tag="OO590_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639167.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTL"
BASE COUNT         1114 a         1211 c         1178 g         1219 t
ORIGIN      
        1 atcgacatgc gcacgaatca gcgccataaa cggtttacca aagcgttcca gcttgcgcat
       61 cccaacgccg ttaacgctga gcatttcgct ggcggtgatc ggcatctgtt cagccatctc
      121 aatcaaggtt gcgtcgttaa acaccacgta cggcgggaca ttactttcat cggcaatcga
      181 tttacgcagt ttgcgtaatt tggcgaacag tttgcgatca tagttgccgc cgaacgattt
      241 ctgcatcgct ttcggtttga gcgccacgat acgcggcacg gcaagttgca aagaggattc
      301 gccgcgcagc accgggcgcg cggcctctgt cagttgtagg gcagaatgct gggcaatatt
      361 ttgcgtcacc aggccgagat gaatcagctg gcggatcacg ctcacccaat gttcatggct
      421 tttatcacgg cccataccat agactttcag tttgtcatga ccatagtcgc ggatacgctg
      481 gttattagca ccacgaatca cttccaccac ataacccatc ccaaaccgct gattcacacg
      541 accaatggtg gaaagggcaa tctgagcatc ggttgaaccg tcgtactgtt tcggcggatc
      601 gaggcagata tcgcagttcc cgcacggctc ctgacgccct tcgccaaaat agttcagcaa
      661 taccagacga cggcaagttt gcgcttcggc aaacgcgccc atcgcattga gtttgtggcg
      721 ctcgatatcc agcaactgcc cctgcggctt ctcttccaga caacggcgca gccacgccat
      781 atcagccgga tcgtaaaaca gcatcgcttc cgcaggcagg ccatcacgcc cggcgcgacc
      841 ggtttcctga taataggatt cgatattgcg cggaatatca aagtggacca cgaagcgaac
      901 gtttggttta ttgatgccca tgccgaacgc caccgtcgcc accacaattt gcagatcatc
      961 gcgctggaat ttctcctgca cgtcggcgcg gacattattt tccagcccgg catgataggc
     1021 cgccgcgcta atacccttgc tttgcaggcg cgcagcggtg tcttctactt tcgcccggct
     1081 gttgcagtag ataatgccag acttaccgcg ctgttcctgc acgtagcgca tcaactgatc
     1141 gagcggtttg aatttctcca tcagcatgta gcgaatattc ggacggtcaa aactgctgat
     1201 ttgaatcagc ggatcgttca gccccagcag gcgcacgata tcctggcgcg tggtgtcgtc
     1261 ggctgtggcg gtcagcgcca taaacggcag cgtcgggaac cgctggcgca actgaccgag
     1321 cgcggcatat tccgggcgga aatcgtggcc ccattgggag atacagtgcg cttcatcgac
     1381 ggctaataac accggattcc agtgcgccag atgctcaaga aagttatcca gcatcaggcg
     1441 ttccggggcg atatacagta agcgaatttg cccggtgcgg cagcctgtca tcacttcaag
     1501 ttgctgttcg cgggtttgcg tcgagttaag gcacgccgcc gccacgccgt tggcctgtag
     1561 ttgatccacc tgatctttca tcaacgaaat cagcggtgaa acaaccacgg taaggccgtt
     1621 tagcaataag gcaggaattt gatagcaaag ggattttccg ccaccagtgg gcataacaac
     1681 caggcaatcg cgaccggaaa gcacagtgtc gataatttct tcctggccgg ggcgaaactg
     1741 ttggtagcca aaggtttctt gtaaaacctg tttagctccg gactccagat tcaacacttc
     1801 cgcctgcgcc acattcaccc cgattgcctg aaataaaaac aggcgctatt ttcagcgcct
     1861 gcgcgagaaa ctgcaatgct tttagtctct gagagggtaa tcctggatgc tggattccag
     1921 atttgccaac cactcaaccg ttcatcaaaa caagtcgttt agcataaccc ctacaccgac
     1981 acgggtctgg ttgaagttat agtcgatgag cgattctcca tagccgctgt aaacctgagt
     2041 ataaaggcgc acatgtttgg tgatcgggta acttaagcct aactccgcgc cgccgtagcc
     2101 ggtgttccag ttgtactgtc ctttcgcact gagcaccgca tcaccgaggt gatagccgat
     2161 tttaagctgg tagtaaccca tatatttggt gatatccggg ttatcgtcag tattacccac
     2221 cacataccac ggcttcactt ctaccagcca gttaccgttt tctgccatca ggcgagtata
     2281 aaggcggttc cagctgcggg aggtcgggtc tgaacgcccg ttagagtcgt ggttataccc
     2341 catctccaca tcgcgcagcg tccaacctgc aaaacggtaa tcggtggcaa aaccgaggaa
     2401 caattgcggt tcgtagttgg tttcacgaaa cggtgaagac tcttcgctat tggacagttg
     2461 ccaccaggat ttttgcgtat aagacgcacc caacaccgag tttgggccta aaatcccacg
     2521 ccacagcgga aatgccaggc tcaactgaaa ctttacttca tccttacgcg cattttccgc
     2581 ccagtcgtaa ctggcaatcg cttctttatt cagatcgctg gtttgggtgt agatgaggta
     2641 gttggtgtca taaggataga gcgtgaacgg attgtcatgc tcctgcagca tattggcgat
     2701 aatactgcca cgcaccgctg gcgcgtcatg cacctctttc accgttgcct cttgtgcata
     2761 tactgccata ggcaacataa acaccggcaa caaccagccc tgcagagtcc gcatcggtcg
     2821 tgttctccgt aacgaattat tgataaggtg aatgtttttc tgcgggctat tttacatact
     2881 taactccatt gccgttagtt atcccttcct aaagtagaaa ccaacaacaa agaagcataa
     2941 aatcaacatt tcattaacca atgaagtgta aggatgatat gtctgccgta ctgaccgctg
     3001 aacaagccct gaaattagtg ggtgagatgt ttgtttatca catgccattt aaccgcgcat
     3061 tggggatgga actggagcgt tacgaaaaag agttcgcaca gctggccttt aaaaatcagc
     3121 cgatgatggt gggtaactgg gcgcaaagca ttttgcacgg cggagtcatt gcgtcggcgc
     3181 tggatgtcgc cgccggtctg gtgtgcgtgg gaagtacctt aacccgccac gaaaccatca
     3241 gtgaagatga actacgccag cggctatcgc ggatggggac cattgatctt cgcgttgatt
     3301 atctgcgccc aggcagaggc gagcgtttta ctgctaccag tagcctgttg cgtgcaggca
     3361 ataaagtcgc cgtcgcccgc gttgaattac acaatgaaga acagctttat attgccagtg
     3421 ccaccgccac ctatatggta ggttgaagac ataaaagcag gtaaactgct gttaccaatt
     3481 aaaaatgagt attcccgatg gatgcaaaac aaacgcggca gggcgtatta ctcgctcttg
     3541 ccgcttattt tatttggggt attgcgccag catacttcaa gctgatttac tacgtgcccg
     3601 ccgatgagat cctgacgcat cgcgtgatct ggtcgttttt ctttatggtg gtgctgatga
     3661 gcatttgccg ccagtggtcc tatttaaaaa cgctgattca gacgccacag aaaattttta
     3721 tgctggcagt ctctgccgtg ctgattggtg gcaactggct actgtttatc tgggcggtga
     3781 acaatcacca tatgctggaa gcgagccttg gttactttat taacccgctg gtgaacattg
     3841 tgctggggat gattttcctc ggcgagcgat tccgccggat gcaatggctg gcggtgattc
     3901 tggcgatatg tggcgtgttg gtccagctgt ggacttttgg ttcgctacct attatcgcgc
     3961 tggggctggc atttagtttt gccttctacg gcctggtgcg caagaagatt gcggtagaag
     4021 cacaaaccgg catgttaatt gaaaccatgt ggctgctgcc cgtggcggcg atttacctgt
     4081 ttgctattgc cgacagttca accagccata tggggcaaaa cccgatgtcg ctgaatttac
     4141 tgctgatcgc cgccggtatt gtcactaccg taccgctgtt gtgttttact gccgctgcca
     4201 cgcgcctgcg tctctcaacg ttaggctttt ttcagtacat tggcccgacg ctgatgttcc
     4261 tgctggctgt gacgttttat ggtgaaaaac cgggtgccga taagatggtg actttcacct
     4321 ttatttgggt ggcgctggca atttttgtga tggacgcgat ttatacgcag cgtagaacgt
     4381 ccaaatcaaa aataaatata ttctgaaaaa atatgccgac tcttgtatga gagtgggcat
     4441 attgccatgc tgacaggaga ctcacacaag gatgtaaaat ttatgttacg tatgtttatc
     4501 ccaacatcta acggtaagat ttccagacgc cgatatatat tttcatttat tttaataaat
     4561 ttcatttttg cctttcttat tatctttttt aacgatgggg aagcaggttt tcttgttata
     4621 gtctctacca tcgcactcca ttatctcgtc atcaacatga attgccaacg actccgtgat
     4681 agtggtttca tatatataaa aatttatgtt tttggtacat ta
//
LOCUS       DAJTYF010000204         4667 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTYF010000204 DAJTYF010000000
VERSION     DAJTYF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.207"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..213
                     /locus_tag="OO590_004018"
     CDS             <1..213
                     /locus_tag="OO590_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6639168.1"
                     /translation="PQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEEL
                     SNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            356..577
                     /locus_tag="OO590_004019"
                     /pseudo
     CDS             356..577
                     /locus_tag="OO590_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(598..660)
                     /locus_tag="OO590_004020"
                     /pseudo
     CDS             complement(598..660)
                     /locus_tag="OO590_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1110..2522
                     /gene="ospC1"
                     /locus_tag="OO590_004021"
     CDS             1110..2522
                     /gene="ospC1"
                     /locus_tag="OO590_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6639169.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2626..2775)
                     /locus_tag="OO590_004022"
                     /pseudo
     CDS             complement(2626..2775)
                     /locus_tag="OO590_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2851..4548
                     /gene="ospD3"
                     /locus_tag="OO590_004023"
     CDS             2851..4548
                     /gene="ospD3"
                     /locus_tag="OO590_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6639170.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1627 a          836 c          868 g         1336 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
      121 ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt
      181 tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac cgggaaaccg
      241 gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc cgacaaacat
      301 ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg aggaaatgga
      361 cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc aacaggacac
      421 tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg cttatgcttc
      481 cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt tcggtaaaag
      541 ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg tgatctacgc
      601 cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact cataagggaa
      661 gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc tctattaaaa
      721 aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga tttcattgca
      781 gaatacacat tccattaact ggaagcactc cataagagga atcagagaaa gtatcgtttt
      841 tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat tatgaaaata
      901 atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat tttgaaatat
      961 ctggcatcac ataacatcaa aagtatataa tacccttatc taattacatg cagtatattg
     1021 tatatatact caaaaattgt aattcatacc ctgctatata ttgtagggaa agatattaat
     1081 taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg aactcagcaa
     1141 atacccaatg caatatagat tctatggata acagattaca tacattgttt ccaaaagtga
     1201 catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta aaagatagtg
     1261 caaatattat taaagatttc tttaggaaaa ctatagcagc acagagttat agtagaatgt
     1321 tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca tcagacgcta
     1381 aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat atatctgaaa
     1441 taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg ctaattatta
     1501 attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa attttaaaca
     1561 ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac acatacgctg
     1621 atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa atctctaacc
     1681 atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt gcaaatgcgt
     1741 atatcattga tcatgactct ccatatggat atatgacatt aaccgatcac tttgataatg
     1801 ctattccacc tgttttttac catgagcacc aatcattttt agataaattt tcagaggtta
     1861 ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta ccaatattca
     1921 atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt agaaaaagtg
     1981 aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg gatctggata
     2041 gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta agtacagaaa
     2101 acttcaaaaa agttaagatt agagaaatat ccttagagga ggctgttaca gcatctaatt
     2161 acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct ctttttcttt
     2221 cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt gagataacta
     2281 gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta cttagcgctc
     2341 ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt gatgtaaaca
     2401 caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata aaatatgaga
     2461 atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat aaatatattt
     2521 aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc gatatattgt
     2581 ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatggggt tcgccccgtt
     2641 gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga gcagcccccc
     2701 tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggg gacaaaccac
     2761 atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta agtctgcgtc
     2821 acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat cccatcaagg
     2881 aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac aatccagtct
     2941 ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt attaaatcta
     3001 aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt agcttcctac
     3061 tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt tgcttttccg
     3121 gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa tgtgcctgct
     3181 attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat tttcaatgaa
     3241 atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa tcacaatatg
     3301 gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat tcagctttat
     3361 gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa cttagctaaa
     3421 ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg ttatggtctt
     3481 atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat gtcctccata
     3541 atcagatggc ctaataattt acttcacccc aaagttattt atcacgcgat gcgtatggga
     3601 ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct ttcagacaat
     3661 acgttagaat tacttttggc agccaaaaat gacgatggtt tttcaggatt gcttttagct
     3721 ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga aacttctgga
     3781 cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg acgaatatcg
     3841 ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata cggaaggctt
     3901 ctcaagaaga gagcaataaa tatcgaatac aataagctga aaaatttgct gaccgcttat
     3961 tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca aaatggacat
     4021 gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct caactcagag
     4081 gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact tctgttagca
     4141 ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa tgaggcaaaa
     4201 cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg tttatctgga
     4261 ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg gaaaatactt
     4321 cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga aggcccaaat
     4381 ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat aaaaacttat
     4441 atgggaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca cttggacaaa
     4501 aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat attatctcgt
     4561 ggtgatacac tgaatttgag gtaaccggct catttaaacc gtctggtctg ttacctccgg
     4621 ttccacaaaa ataatgtcca tcattttgaa gtggacacta tcgtatg
//
LOCUS       DAJTYF010000205         4610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTYF010000205 DAJTYF010000000
VERSION     DAJTYF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.208"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..76
                     /locus_tag="OO590_004024"
     tRNA            1..76
                     /locus_tag="OO590_004024"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Thr,seq:ggt)
     gene            116..231
                     /gene="rrf"
                     /locus_tag="OO590_004025"
     rRNA            116..231
                     /gene="rrf"
                     /locus_tag="OO590_004025"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(294..1052)
                     /locus_tag="OO590_004026"
     CDS             complement(294..1052)
                     /locus_tag="OO590_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6639171.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1060..2163)
                     /locus_tag="OO590_004027"
     CDS             complement(1060..2163)
                     /locus_tag="OO590_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6639172.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2173..3343)
                     /locus_tag="OO590_004028"
                     /pseudo
     CDS             complement(2173..3343)
                     /locus_tag="OO590_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3411..4436)
                     /locus_tag="OO590_004029"
     CDS             complement(3411..4436)
                     /locus_tag="OO590_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6639173.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1266 a         1203 c         1096 g         1045 t
ORIGIN      
        1 gctgatatgg ctcagttggt agagcgcacc cttggtaagg gtgaggtccc cagttcgact
       61 ctgggtatca gcaccacttt ttaggttaaa gttcggcaga ttagaaaaga atttgtctgg
      121 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
      181 cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccaga catcaaatca
      241 agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt caattaatgg
      301 attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa aaattcatca
      361 ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc agcgacggta
      421 cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg cgccagccca
      481 atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt tggttcatca
      541 aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg ttgctgctga
      601 ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac ccgctcaagg
      661 taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca aatcggtgcc
      721 agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg aaataccatt
      781 cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc gatgccatct
      841 acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat ggttgtcgat
      901 ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac ggttaaattt
      961 atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag cgtaatcatc
     1021 gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg tacgcccggt
     1081 gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa agatccagta
     1141 gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg gatcaacggt
     1201 tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta ggctggtatc
     1261 tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca acgcctgtgg
     1321 cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg ccgcttcata
     1381 ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat atgctgactg
     1441 aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc cttctgccat
     1501 aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta cgccgcgcca
     1561 gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc ggcgacctaa
     1621 tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa gaattaaagt
     1681 tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa aaccgccata
     1741 catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat agcgaccgcg
     1801 atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca gtaatgcgag
     1861 gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac caaatcgttc
     1921 gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg agccaaccca
     1981 attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca tcaaccatat
     2041 gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc gcacccacac
     2101 catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga atacttttgt
     2161 catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat tcatcagcag
     2221 cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg tctcgatggc
     2281 ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg gatagccaat
     2341 agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta acggcggaat
     2401 aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt tgggtaaccc
     2461 cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag cgcggataat
     2521 ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg ccagttcagg
     2581 aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat cccagtgtag
     2641 tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca ctgcggcgat
     2701 cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa agcggaatag
     2761 cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa atccgtctcc
     2821 cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat caaacgcgct
     2881 cacagcttgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga agaagatttg
     2941 caatattacg gaagatctca atataaatcg tggaaagctt tcgtagcagc cagttatccg
     3001 aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata cacaatgcag
     3061 aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg tcgccctgct
     3121 ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga aaggcaaaac
     3181 ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac caaccaacaa
     3241 tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc gtagggttgg
     3301 caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc tcggaaagca
     3361 taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac ttaacgcacg
     3421 ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg tttaattttc
     3481 agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc cacctgtttg
     3541 atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc gccttcttta
     3601 cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc gacgttctgg
     3661 gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg tacaatcgag
     3721 aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga gataacctcc
     3781 ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata cagttgcgac
     3841 tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga ttcgtcagag
     3901 cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc ggcgacgttg
     3961 agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc tttcgcgctt
     4021 tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt gacgccggta
     4081 aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg ggagagcaaa
     4141 tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg ggtatatttc
     4201 actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc aacatcaata
     4261 cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc actgatcccg
     4321 cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg cgcctgattt
     4381 gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt tttcataatg
     4441 cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt tacctgtctg
     4501 tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc gcgtcgcaat
     4561 aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtgcg
//
LOCUS       DAJTYF010000206         4577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTYF010000206 DAJTYF010000000
VERSION     DAJTYF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.209"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            437..2089
                     /locus_tag="OO590_004030"
     CDS             437..2089
                     /locus_tag="OO590_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6639174.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO590_004031"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO590_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO590_004032"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO590_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3030)
                     /locus_tag="OO590_004033"
     CDS             complement(2596..3030)
                     /locus_tag="OO590_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HCR6639175.1"
                     /translation="MDTFKRRNVATSFSFLGYDFKVRTLKNFKGELYRKCMPGASNAA
                     MCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFSYRLWSAMQSR
                     LLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            complement(3207..4516)
                     /gene="ltrA"
                     /locus_tag="OO590_004034"
                     /pseudo
     CDS             complement(3207..4516)
                     /gene="ltrA"
                     /locus_tag="OO590_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1217 a         1109 c         1055 g         1196 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggtacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgg
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagaaagg agaaactcgt
     3001 agcgacgttt ctccttttaa acgtgtcaat gtaggcgata ttcgtcttcc ctgcgttcag
     3061 gaccaacccg acttccgaga agcgcaccga taacctgttc ttcagccttg ttgcatcact
     3121 cattcgtgaa caatgtacta caagtcgagt cacccgaggg aacctccccc tctggtgctc
     3181 acggaaccgg acgtgaacct ctcagctcat ccggctcttc tcattgaggc ctgtccccat
     3241 agtaacggcc agtgtgcaaa taacctcgtt tcactccgcg caacacgggc cagccagtgg
     3301 ctggcccgtc gtcggtggcc cctaagtcgc ttgtgtttac ggggcagcca taacacgagt
     3361 ttgcgatcga tttgcctcag cgtcagatac agtgctgatt tataaaaagc accgtagtac
     3421 tttatccagc ctttgatttt cgcattgaac atatgcgcca gctcgtacaa ggacttgtca
     3481 ctgcgattct cctcacctcc tgacggattg cttttcctgc tctggcactc atcgccggac
     3541 tgaagttaac gaaggtctta ccccagcgat tcatcgacag acgcggccgg aatgtatagc
     3601 ccagaaagtt gaagctcgtt tccgggtagt ccccccgtcg gtcagcatcc ttacagtaaa
     3661 ctattcgtgt tttttgcgga tgcagctgca gcccgcattg cgcaagccgc tgcctcagtc
     3721 cacttatcag agcgtcagcc tgccactggc tgtgacagtg gcatacaaca tcatcggcgt
     3781 acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag gcatagtgca
     3841 ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct cgaccccgtg
     3901 ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca atgtacatca
     3961 ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca tgatcgatat
     4021 catcaaagaa gcctttaata tcgacttcca ccacccagtc aaacttccag cagcgtttcc
     4081 tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac gagtctgcat
     4141 ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg gcgatccggt
     4201 ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga atgtccacac
     4261 gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt ttgtagagat
     4321 tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct gccgccccac
     4381 ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca ggaaatggct
     4441 ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc gccgcaccat
     4501 cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc gctactacgg
     4561 tttactccgt ccctgta
//
LOCUS       DAJTYF010000207         4504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTYF010000207 DAJTYF010000000
VERSION     DAJTYF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.210"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..243
                     /locus_tag="OO590_004035"
                     /pseudo
     CDS             1..243
                     /locus_tag="OO590_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            736..1482
                     /locus_tag="OO590_004036"
     CDS             736..1482
                     /locus_tag="OO590_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6639176.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1497..1919
                     /locus_tag="OO590_004037"
     CDS             1497..1919
                     /locus_tag="OO590_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6639177.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1977..2333
                     /locus_tag="OO590_004038"
     CDS             1977..2333
                     /locus_tag="OO590_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639178.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2426..2644
                     /locus_tag="OO590_004039"
     CDS             2426..2644
                     /locus_tag="OO590_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6639179.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2646..3008
                     /locus_tag="OO590_004040"
                     /pseudo
     CDS             2646..3008
                     /locus_tag="OO590_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3008..3244
                     /locus_tag="OO590_004041"
                     /pseudo
     CDS             3008..3244
                     /locus_tag="OO590_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3437..3673
                     /locus_tag="OO590_004042"
                     /pseudo
     CDS             3437..3673
                     /locus_tag="OO590_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3673..4038
                     /locus_tag="OO590_004043"
     CDS             3673..4038
                     /locus_tag="OO590_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6639180.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT         1205 a          955 c         1281 g         1063 t
ORIGIN      
        1 aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca tcccaaaccc
       61 ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg gagattatct
      121 ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc tgctggcctg
      181 gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg gcggattgat
      241 atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg aaattctccg
      301 cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt cttgcggcgc
      361 agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc tgttcgccag
      421 aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagatac actctggcat
      481 gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct gggcgctaac
      541 ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga gcaggtgatt
      601 cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt tcggcctgag
      661 acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc gccaggcttc
      721 agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg tatccgcaga
      781 ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg cgagtggcaa
      841 cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca gttgcgggtg
      901 gaaaaaattc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc gtttgcgaat
      961 taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc catagctgac
     1021 gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac cggaaaaaat
     1081 catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt gattatcgtc
     1141 accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa atccggtgaa
     1201 aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat tggcatgcag
     1261 cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg gacggcatcg
     1321 ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac acttctcggc
     1381 gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt taactgggag
     1441 agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga ggatttatgg
     1501 cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg gaactcgtgc
     1561 atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc agtcgtgcgt
     1621 ctgtcggtcg cttatgcatg gaactggtcg caagtggtga tgtatataat tctggctacg
     1681 gggtatttcc ctctgaacag gctcataaag actggcaaaa cgcccgcaaa aagctatcga
     1741 gggtaaaggt gaagaaaccg gttgtggttg atcctgacct tatctggtca ttaccagacg
     1801 gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc cggaagagcg
     1861 aagctatgca gcgcgtgctg gcgttttatc aggggaaatt tcaggaggcg gtactgtgag
     1921 taaaattagc tatcaggctt caattaccgc tggtattcgc atcaaaggag aggagcatgg
     1981 aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa gaggatgacc
     2041 cacgtaatca ggttaatttt ctttatgaac catcggaaag accatattgc catcacgcct
     2101 ctgtccgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt gcggttgtgg
     2161 agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca gatgatgtaa
     2221 ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg atacagattg
     2281 agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa taattaaatt
     2341 tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca aatcatcagg
     2401 agaccgcccg aaagggcggt aatgaatggt tacattattt agaaaaaaat atccgcgaaa
     2461 gagtagaaca acagaattcc tgtttctcat tctgtttatc gtgttgatga caccgatatc
     2521 cccgctaatt tttgtctgga taatcggaaa aataattgag ccagttattg aattgtataa
     2581 cgacgtggta tgggcgtcat tcaacacact gcacaataaa attaatccgt ataaggaaag
     2641 ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc ggtggtcgct
     2701 gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta aaaccggtca
     2761 ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac atggtaccag
     2821 aatgccaccc atgcaatctg cacaagcatt gcagtagcct agaagattat cgacgaatta
     2881 tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg
     2941 gattggttga aatgaaatct gacccggttg tgttctggtt tgaaaaatat caagaagggg
     3001 ctacggcatg gccactatta ccaaagagcg actgcaatgg ctggctaaca tttctggccg
     3061 cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta tcgctctggc
     3121 atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca tgtgtattga
     3181 cgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc tttatgctgc
     3241 cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga aggtgacgtt
     3301 tactcaagtt gaccaggaat ctaatacgtt cactgctata tattttgaca aagcggaagt
     3361 tgagcgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa caccgcttta
     3421 cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc
     3481 actgtcagat tcatcgcttg gctctggtga aatcgaagaa attaaaaccg gtttgcgtgc
     3541 tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa
     3601 cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa
     3661 gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag
     3721 cagaaatgta ttgggcaact ttgtaaaatt ccaccaggag atatggttga aatcgtggaa
     3781 atggccatgc gtaaggctgg caactctccg gtaactctgg ctggctggat aagctgtagt
     3841 gatgcagttc ctgctgaata ttgcgatgtg attcttctcg atgatctcgg gaatgtattc
     3901 cccggttcct gggataaggt tttttgcccc actcgtggcg ggaataagat ggcttttgtg
     3961 gacaaagacg gcgttgaagt agagagctca actcactgga tgccgctacc ggaaccaccg
     4021 caggaggtga atcaatgacc tggcctgagg cattcaccac ggtaggaatt gcgatggcgg
     4081 tggcgctggt ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc ccggattgat
     4141 ggtccggggt ttttgaagga aacaaaacag aaacagcaat tgccgttacc tgttgttacc
     4201 atggcaagta aacgtatctc aggcgagcgc attgcgccgt tctgacgcag ataaattagc
     4261 ctggatagaa ggtgctggca ataaaaaata gcgttttctt atcggtatcg gtaagattgc
     4321 tgcgggtgct tgaggctgtc tgcctcgggc atgccactgt aaggcagaca gagaaaagcc
     4381 ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc aatctatgtc
     4441 tcacaaatgt aggttagcct cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca
     4501 gcaa
//
LOCUS       DAJTYF010000208         4440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTYF010000208 DAJTYF010000000
VERSION     DAJTYF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.211"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..213)
                     /locus_tag="OO590_004044"
     CDS             complement(<1..213)
                     /locus_tag="OO590_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR6639181.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OO590_004045"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OO590_004045"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6639182.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OO590_004046"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OO590_004046"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6639183.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OO590_004047"
     CDS             2894..3634
                     /locus_tag="OO590_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6639184.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="OO590_004048"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="OO590_004048"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6639185.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4192..>4440)
                     /locus_tag="OO590_004049"
     CDS             complement(4192..>4440)
                     /locus_tag="OO590_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639186.1"
                     /translation="SKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGS
                     IPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1101 a         1106 c         1232 g         1001 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtctt acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tctggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
//
LOCUS       DAJTYF010000209         4433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTYF010000209 DAJTYF010000000
VERSION     DAJTYF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.212"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..716)
                     /locus_tag="OO590_004050"
     CDS             complement(<1..716)
                     /locus_tag="OO590_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6639187.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDI"
     gene            complement(927..2324)
                     /gene="ccp"
                     /locus_tag="OO590_004051"
     CDS             complement(927..2324)
                     /gene="ccp"
                     /locus_tag="OO590_004051"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6639188.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2729..4378
                     /locus_tag="OO590_004052"
     CDS             2729..4378
                     /locus_tag="OO590_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6639189.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDLYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVSREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1127 a         1129 c         1049 g         1128 t
ORIGIN      
        1 tcgatgtcga taccggtgtc ggcctggaat ttatccagcg catcgtgcag cggttgtggg
       61 aactcatagt taccggtgta ggtcacgccg aaagtcggca ccacgccgat ggtgttttcg
      121 tcacaggctt caatcatgcg tttcgggtcc ataaacaact gaccggggcg catagggatc
      181 tcacgcagct ccacatccca gtagcgggcg aatttatgcc agcagatttg taccggaccg
      241 cacactaggt ttggtttatc cgttggtttg cctgcagctt ccatacgctt gcgccaacgc
      301 catttcatcg ccatcccgcc gagcatacag gcctcggaag aaccaatggt gttggtgcca
      361 acggcctgac catttttcgg cgcaggcgca tgccacagat cggcaaccat atttacgcaa
      421 cgcaggtcga tggctgcgga ttgcggatat tcttctttgt cgatccagtt tttattgatc
      481 gacaaatcca tcaatttatg gacgttttcg tcgtcccagg tctggcagaa agtggctagg
      541 ttctgacgag cgttgccatc aagatataat tcatcattga taatctggaa tgcgacatca
      601 tcgcgcattt cgtgcagcgg aaatcgtttt gactccgcga tagtagaaat ggcctttgcg
      661 ccaaaacgtg aatcgagtag ttctgagcgg aaatccgtta acagcttctg gtccatttcg
      721 aactccttaa atttatttga aggcaataaa aaagtaggat ttatccgcaa tggaagcaag
      781 gcattacagg cttaatttaa ataacaaaat cctaagcaga aaaacgactt tttataatat
      841 aaacatttaa catgataata ttaaaaacag actttattat cgtaataaaa atcgcccaaa
      901 cattcctgtt cgggcgatca aattaattat tgtttatcct gcatatacgg cgtgtacacc
      961 ccgttcagac tgtgcaggaa agctacgata tcatccacat cctcctgcgg cagctctttg
     1021 cctacctgat agcgcagcat cagtttcacc gccccgtcca gcgtcggcac gtcaccgcga
     1081 tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg gtactttctg acgcaattta
     1141 tcacgctctt ctttagtcac attcatacga ccaatatccg ccgccgtaat ttccccaaag
     1201 ttaaagtctt ttttcagcac caacggttca aaggaacgtc cgccgagaat aataccacca
     1261 tgacaagttg cacatttatt atctttaaat aattgatagc cttttttctg ttgcgccgtc
     1321 agcgcatttt catctccacg caaccattta tcaaatgggg aatccggcgt aattaatgtt
     1381 ttctcaaatt cagcaatggc atcagtaata ttttcgccac tgaaaccttg cggataaact
     1441 tcgaggaact gcgctttaag ctgcggatct ttttccagct tagcaataat ttcgtcccag
     1501 gatttcgacg ccatttcaat cgggttcaac ggcggtccac ctgcctgatc ctgcaatgtt
     1561 gccgcacgac catcccagaa ctgctcaacg ttaaatactg agttaaatac cgtcggcgca
     1621 ttaatcggcc caactgcgcc accaacacca atcgatgttt ttctgccatc gacgcccccc
     1681 gcattcaacg tatggcaatg agcgcatgaa atggtgctat cagccgataa acggggatcg
     1741 tgatacagcg caaagcccaa cgccactttt tgcgcatcgg taggcagttt ttgcgggatg
     1801 ggctgcaccg gttcattgcg atgctccgga gcagtatcat tgctggcgta atattccgcg
     1861 cgctgttttg caatccaggc cagtatttcc gcccgctctt catcactcac cttacccgcc
     1921 cagtgtagcg cggtataacg cgttggtggc atagtttcat actgcatcac ccattcaatc
     1981 ttattcagat cgctttgcga aacgggttta tcagccagca gtgccgcacg cacggcctcg
     2041 aggttaaaag atttatatcc aagcttaatg tcgtaatcca tcaactgttt cgcgccagga
     2101 atataataat aggcgggtaa ttctgccgaa ggcgtgtggc aatagtcgca tcctttttcg
     2161 cggagaaagc ctaaaacctt attattttca ctgacagcag atgcctgaac atcggcctgt
     2221 ttactgcgtt tattatcgtg gtaccacaca taaccagata accctaaata gcaaatcgcg
     2281 acgccagcca ggccgatcgc ggtaatacgt gagaccattt tcattattct tccctcacgg
     2341 tggtgagtta ttgtgaccca gaaaattttc cagaatgctg caaacgtctc tgcgagacaa
     2401 agccatcata aaaaacaggg agatacgctt tttgatagga atcaatcaat tacatgaata
     2461 taaactatca ataagatagc ctgtgcctat tacccagatc aggaatggca tatcatgatt
     2521 gcggcgagag caggattgaa tgttctgaaa atgaaaaaca acaccaaatc cgtattttta
     2581 taaattccat tgatattagt gcgttatatt ttttgacaag ttaataaacg tgatccaccg
     2641 cacgctttgt cgcccaccag gcggagcgaa tgactaccct taaagaaaag cccgataatt
     2701 agcgacgaat ttcggaggtt ggatccttat gctcaatcag aaaattcaaa accctaatcc
     2761 agacgaactg atgatcgagg tcgatctctg ctatgaactg gacctgtatg aattaaaact
     2821 ggatgagatg atcgaggcag aaccggaacc cgagatgatt gaagggctgc ccgcctctga
     2881 tgcgctgacg cctgccgatc gctatctcga actgttcgag catgttcagt cggcgaaaat
     2941 tttccccgac agtaaaacct ttcccgactg cgcacccaaa atggacccgc tggatatttt
     3001 aatccgctac cgtaaagtgc gccgtcatcg tgattttgac ttgcgcaagt ttgttgaaaa
     3061 tcacttctgg ctgccggagg tctactccag cgagtatgta tcggacccgc aaaattccct
     3121 gaaagagcat atcgaccagc tgtggccggt gctaacccgc gaaccacagg atcacattcc
     3181 gtggtcttct ctgctggcgc taccgcagtc atatattgtc ccgggcggcc gttttagcga
     3241 aacctactat tgggattcct atttcaccat gctgggactg gcggaaagtg gtcgggaaga
     3301 tttgctgaaa tgcatggccg ataacttcgc ctggatgatc gaaaactacg gtcacatccc
     3361 caacggcaac cgcacctatt atttgagccg ctcgcaacca ccggtttttg cgctgatggt
     3421 ggagttgttt gaagaagatg gtgtacgcgg tgcgcgccgc tatctcgacc accttaaaat
     3481 ggaatatgcc ttctggatgg acggtgcaga atcgttgatc cctaatcagg cctatcgcca
     3541 tgttgtgcgg atgccggacg gatcgctgct caaccgttac tgggacgatc gcgacacgcc
     3601 gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa cattctggtc gcccgcccaa
     3661 cgaggtgtac cgcgatttac gcgcgggagc ggcctccggt tgggattact cttcccgttg
     3721 gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc cagttcatcc ccatcgatct
     3781 gaatgccttc ctgtttaaac tggagagcgc catcgccaac atctcggcgc tgaaaggcga
     3841 gaaagagaca gaagcgctgt tccgccagaa ggccagtgcc cgtcgcgatg cggtaaaccg
     3901 ttacctctgg gatgatgaaa acggcatcta ccgcgattac gactggcgac gcgaacaact
     3961 ggcgctgttt tccgctgccg ccattgtgcc gctctatgtc ggcatggcga accatgaaca
     4021 ggccgatcgt ctggcaaacg ccgtacgcag ccggttactg acacctggcg ggattttggc
     4081 aagcgagtac gaaaccggtg aacagtggga taaacccaac ggctgggcac cgttacaatg
     4141 gatggcaatt cagggattta aaatgtatgg cgatgacctt ctgggtgatg aaatcgcgcg
     4201 cagttggctg aaaacggtga atcagttcta tctggaacag cacaaaatga tcgagaaata
     4261 ccatattgcc gatggtgttt cccgcgaagg cggcggtggc gagtatccgt tgcaggatgg
     4321 gtttggctgg actaacggtg tggtacgccg tttaattggt ttgtacggcg aaccataata
     4381 tttttacagc cagccgctaa cttcctgctg gctgtaaaat tatcctcttc agg
//
LOCUS       DAJTYF010000210         4263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTYF010000210 DAJTYF010000000
VERSION     DAJTYF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.213"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            158..827
                     /locus_tag="OO590_004053"
                     /pseudo
     CDS             158..827
                     /locus_tag="OO590_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1070..1765)
                     /locus_tag="OO590_004054"
     CDS             complement(1070..1765)
                     /locus_tag="OO590_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6639190.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1758..3185)
                     /locus_tag="OO590_004055"
     CDS             complement(1758..3185)
                     /locus_tag="OO590_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6639191.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3196..3915)
                     /locus_tag="OO590_004056"
     CDS             complement(3196..3915)
                     /locus_tag="OO590_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6639192.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1142 a         1099 c          877 g         1145 t
ORIGIN      
        1 ccagatatgg ggaccgaatc gttatataaa acactgaggt aactctcatg cttcatatcc
       61 agtattcatg atgctggttt tttgctgata tttaacccga acattcctga tacagactat
      121 taatacaaca ttatcttcga cagattttag atatattatg agtgaacaaa tcaaacagga
      181 tatcgatctg attgagattt tatttcatct gaagaaaaag attcgtgtta tcctttttat
      241 tatggctatc tgtatggcaa tggtgctgtt gtttctgtat atcaataaag acaatacaaa
      301 agtgatttac agccttaaaa taaaccagac aacgccaggt atacttgtta gctgtgatag
      361 caataataat tttgcctgtc agaccacaat gactgaagat gttattcagc gaattactac
      421 attttttcag accagcccag atatcaagaa cagagaaata aggctggaat ggtcaggaga
      481 taagagagat ttaccaactg ctgaagagga aatatctcgc gtgcaggcgt ctattatcaa
      541 atggtatgcg tcagaatacc ataatggcag gcaagttctc gatgagatac aaacgccttc
      601 agcaattaac agtgagcttt atacaaaaat gatatacctg accaggaact ggtcattgta
      661 tccgaacggt gatggctgtg taactataag ctcaccagaa ataaaaaata aataccctgc
      721 tgccatttgc ctagctctgg gatttttttc taagcattgt aatttctgta atgttttgcc
      781 ttgtcaaaaa aatggtagat gaataccaac aaaactctgg gcagtaataa acagcattct
      841 gatagagagt gcctgtataa agctgaagtg acaatgaccg ggaagggaac ccgttttcag
      901 gaatggatgc ctgtcttttt cactgtaaat atcctggcat tactaatgcc tggatattta
      961 cagaagagca gcataaccat gacggaacgg ggttaagtca ggttgtcctt aacgctattt
     1021 tcaacctcgt atgccttctt caggtttatg ttcagatctc atatctctct cagcaatcct
     1081 caataatcag atacaccgct ttcaccggac catgaacccc gacgactttg ataagctcaa
     1141 tatccgccgt tgagctggga ccactgatga tgttaataca tgacggcatt cgttcaccgg
     1201 cctgcgcttt ctgatgcaat ttttctgcga gttgcgctac acgcggcagg attgtgcttt
     1261 tacgcaggat aaaaagagaa gattccggga gcaggctcaa tgaacgcccg cgctcggcgg
     1321 cggaaaaaag aaccacgcct cccgattcgg ttaaaccata ttcagcatac acgacaccca
     1381 ctttagcctg ctctgcctgc gagatattct cggcaccttt cgccggatcc caaacaacgg
     1441 cattgcattc ctgctgcaaa cgttcactaa tccccaattc ctccagcctc gtgtcaccgc
     1501 taatcacgac cgactgatct cccagctctt tacacagacg tattgcagct tctgccgcct
     1561 tcgcctcgct ggtcagctca cagcgcgtca acataacatc gctggcaaac tgaataaacg
     1621 cgtcacagcg ctgctgttgg ttaagttggg taagccgctc gttagcatag ttgttaagcg
     1681 gcgcatcttc tgcttgcggt tcaagtcgca gcgggcgacc cagtgcctga gcaacgttat
     1741 tcaaaaattc gctccgatta tccattcttt ttctcctgcg cctgatgttt cttaaaccaa
     1801 ctacggaaac tctctccgtc agcttcagga agatcgcgtg cttccatcca gtcgctaatc
     1861 gcgccaaatt tgagtggtgt tttaccgcca ttgataaacc agcttgccgc atgagcgccg
     1921 gccatcatcc cgactttcca caatcctgga tgactattgg cataagcgaa catttttatc
     1981 gcccgttgct ctgcttttgc ggtgatccct ttttcagcca tcacccgacg atgacgcaaa
     2041 atcagttttg acagcggaat acgcaccgga cacacgctgt cacaagcagt gcataaagag
     2101 caggcgtagg gtaaatcttt aaaatcttta tagccgccaa gtagcggaga aatcaccgca
     2161 ccaattggcc ctggataaat agagccatat ccatgaccgc caatatggcg atatgccgga
     2221 caagtattca tacaagcccc gcagcgaata cagcgcagca catcccgaaa ttcagaggcc
     2281 agcacctcag aacgcccgtt atcgacaata accagatgaa actcttcagg accatcaacg
     2341 tgccccgctt cgcgcggccc tgtcagccag gtgttgtatc ccgtcaaacg tgcaccaacg
     2401 gcactgcgcg ccagcatggt gatcaataca tctacctcgg caaacgtggg ggcaatacgc
     2461 tccattccca tcactgcaat atgcgtttta ggcagcgtgg tacacattcg cgcattacct
     2521 tcattggtca ccaggcatac cgaaccggtc tctgccaccg cgaaattaca gccggtaata
     2581 cctatttcag cactgaggaa atcttcacgg attttttgcc ggatgaataa ggtcatcgct
     2641 tcaggcgttt ccggcccctc atagcccaga cgttcgtgta gcactcgacg gatctgatgg
     2701 cgatctttat gaattgccgg gaccacaaca tgagatggcg gatcttgatc cagctggaga
     2761 atatattcac ccagatcggt ttcaatcacc tgaatgccag catcctgcaa cacatgattg
     2821 acaccaatct cttcggtcac catcgattta gatttcacca ccttccgggc atttttgcgt
     2881 tgggcaacct gtaaaatgta gcgggtagcg tcttctttgg tttttgcaaa atagacgtga
     2941 ccgccgtttt gcgtcacttt ttctgagagc tggtacagat aagcgtcgag attactcaaa
     3001 acatgatcac gtatctgggc ggcccgatcg cgccactcct cccagtgccc caattcatcg
     3061 accatttttt gccgatttgc cccaatacgc tgctgcgcgt ttgccaccgc tttgcgcatg
     3121 atcggatctt caatttgctg acggatgcgt gtcttaaaat ctgtattact ggttttgatc
     3181 gacatcttta tatcctcagc ggctcatcaa cacttcagca atatgcatca ctttgacttt
     3241 ctgcccttcc cgttgtaatc gcccactgat gttcagcagg caactcacgt cagcaccaat
     3301 taaatactca gggcggactt ccatcaggtg cacaaccttt tctttcacca tctcgccgga
     3361 tatttcggcc attttgaccg agaacgtgcc gccaaatccg cagcaggtat cctgttcagc
     3421 aaaggtcaac agctccagtc cacgcacatt tttcagcagc gtaagtggct cgtccttcac
     3481 tcccagctta cgggccaggc tacaagatgg gtgatacaca gctctccctt gcaaactggc
     3541 acctacatcg actaccccta atttattaac aataaaagag gtgagatcct gcatacgcgc
     3601 ggcaaccttt tcggcacgtg atgcccattc aggttcatcc gccagatacg tcgggtaact
     3661 ttttacggca taggtgcaag agccagccgg tgaaataatg ggatcgtcgt tatcctccag
     3721 tgcggcgatc agatttttca tccctggaat cgcttctttg atataaccgc tattgatcgc
     3781 aggctgaccg cagcatccct gtttctccgg gaaatttacg cgacagccga gtttttccag
     3841 tagcagcacg gagtctcgtg ccattcttga tttcagggcg tcaccaatac aggtgacaaa
     3901 gaaattgaca ttcacaacta atactccatt acttcatgcc catttatgcc cgatgtttcc
     3961 cccccaccgg gggatcaatc atcaataaat atggcgttct ttttaaattg gctagcaaca
     4021 taccacccct catttgtatg acaaatgata atctttcatc caaacttcaa atgcaataac
     4081 taattttatt tctttcggca tcgctcacat tttatgggga aatcgacgat catttttggc
     4141 ataagaaatc catttaaaaa tggctacatc ccttatacct attcagagct acttattcag
     4201 gggttattga gatatacact gatggtgtac aaaaaaatca ccgacaataa tcacgtatca
     4261 agt
//
LOCUS       DAJTYF010000211         4109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTYF010000211 DAJTYF010000000
VERSION     DAJTYF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.214"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            199..792
                     /gene="tetC"
                     /locus_tag="OO590_004057"
     CDS             199..792
                     /gene="tetC"
                     /locus_tag="OO590_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6639193.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            complement(905..2110)
                     /gene="tet(B)"
                     /locus_tag="OO590_004058"
     CDS             complement(905..2110)
                     /gene="tet(B)"
                     /locus_tag="OO590_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6639194.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            2192..2815
                     /gene="tetR(B)"
                     /locus_tag="OO590_004059"
     CDS             2192..2815
                     /gene="tetR(B)"
                     /locus_tag="OO590_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6639195.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(2793..3479)
                     /locus_tag="OO590_004060"
     CDS             complement(2793..3479)
                     /locus_tag="OO590_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6639196.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(3487..3873)
                     /locus_tag="OO590_004061"
     CDS             complement(3487..3873)
                     /locus_tag="OO590_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR6639197.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            complement(3866..4081)
                     /locus_tag="OO590_004062"
     CDS             complement(3866..4081)
                     /locus_tag="OO590_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6639198.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
BASE COUNT         1352 a          864 c          737 g         1156 t
ORIGIN      
        1 aatccataat agaacatctt tgataaccgt tgtatgaaac tgcttatcat catatcttaa
       61 ttggatgtta ttagctttat tttgatagcg agaatgctgt tcaatataca taaaataacc
      121 taaatgttct taagattgtc acgaccacat catcatgata ccataaacat actgacggta
      181 tgttatttta aatctatcat ggaaaataaa aatcatcaac aagaaaattt taagagtacc
      241 tatcaatcac tggttaactc agcacgaata ttgtttgttg aaaaaggcta tcaagctgtt
      301 tcaatagatg agatctcggg aaaagcgttg gtgaccaaag gtgcctttta tcatcacttt
      361 aaaaataaaa aacaattact cagtgcctgt tataagcagc aattaattat gattgatgcc
      421 tacatcacaa caaaaactga tttaacaaat ggttggtctg ccttagaaag tatatttgaa
      481 cattatcttg attatattat tgataataat aaaaacctta tccctatcca agaagtgatg
      541 cctatcattg gttggaatga acttgaaaaa attagccttg aatacattac tggtaaggta
      601 aacgccattg tcagcaaatt gatccaagag aaccaactta aagcttatga tgatgatgtg
      661 cttaaaaact tactcaatgg ctggtttatg catatcgcaa tacatgcgaa aaacctaaaa
      721 gagcttgccg ataaaaaagg ccaatttatt gctatttacc gcggcttttt attgagcttg
      781 aaagataaat aaaatagata ggttttattt gaagctaaat cttctttatc gtaaaaaatg
      841 ccctcttggg ttatcaagag ggtcattata tttcgcggaa taacatcatt tggtgacgaa
      901 ataactaagc acttgtctcc tgtttactcc cctgagcttg aggggttaac atgaaggtca
      961 tcgatagcag gataataata cagtaaaacg ctaaaccaat aatccaaatc cagccatccc
     1021 aaattggtag tgaatgatta taaataacag caaacagtaa tgggccaata acaccggttg
     1081 cattggtaag gctcaccaat aatccctgta aagcaccttg ctgatgactc tttgtttgga
     1141 tagacatcac tccctgtaat gcaggtaaag cgatcccacc accagccaat aaaattaaaa
     1201 cagggaaaac taaccaacct tcagatataa acgctaaaaa ggcaaatgca ctactatctg
     1261 caataaatcc gagcagtact gccgtttttt cgccccattt agtggctatt cttcctgcca
     1321 caaaggcttg gaatactgag tgtaaaagac caagacccgc taatgaaaag ccaaccatca
     1381 tgctattcca tccaaaacga ttttcggtaa atagcaccca caccgttgcg ggaatttggc
     1441 ctatcaattg cgctgaaaaa taaataatca acaaaatggg catcgtttta aataaagtga
     1501 tgtataccga attcgattgc gtctcaaccc ctacttcggt atctgtatta tcacgtgtat
     1561 ttttggtttc acggaaccaa aacataacca caaggaaagc gacaatattt agcaacgcag
     1621 cgataaaaaa gggactatgc ggtgaaatct ctcctgcaaa accaccaata ataggccccg
     1681 ctattaaacc aagcccaaaa cttgccccta accaaccgaa ccacttcacg cgttgagaag
     1741 ctgaggtggt atcggcaatg accgatgccg cgacagcccc agtagctcct gtgatccctg
     1801 aaagcaaacg gcctaaatac agcatccaaa gcgcacttga aaaagccagc aataagtaat
     1861 ccagcgatgc gcctattaat gacaacaaca gcactgggcg ccgaccaaat cggtcagaca
     1921 tttttccaag ccaaggagca aagataacct gcattaacgc ataaagtgca agcaatacgc
     1981 caaagtggtt agcgatatct tccgaagcaa taaattcacg taataacgtt ggcaagactg
     2041 gcatgataag gccaatcccc atggcatcga gtaacgtaat taccaatgcg atctttgtcg
     2101 aactattcat ttcacttttc tctatcactg atagggagtg gtaaaataac tctatcaatg
     2161 atagagtgtc aacaaaaatt aggaattaat gatgtctaga ttagataaaa gtaaagtgat
     2221 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     2281 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     2341 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     2401 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
     2461 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
     2521 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
     2581 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     2641 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     2701 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     2761 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt cttaaaagca
     2821 gcataacctt tttccgtgat ggtaacttca cggtaaccaa gatgtcgagt taaccaccct
     2881 ttagattcat aaagcgaaaa taatgcggct ccaacgtacc cacctaaatg gaaacggcgt
     2941 tcactccaat ctaaacacgc acaacagatt ttacgtgaat gtttggaagg aacgtcaatt
     3001 cccatttcat gaaaatattg aataccactt aatgtgatca ttgaaccatt ttcagtgatc
     3061 cattgctgtt gacaaaggga atcatagatc ttaacggcaa cttcgccagc taaatgatca
     3121 tagcaagtac gtgcttttcg taaatgcact ggcgtggaaa ctttggcatg tacgccatgg
     3181 tttaaggaga tccccatcat actttccatc aattcagcaa tatcttttcc tgctagccga
     3241 aaataacgat gcttgccttg agctactact gtgattagct ggcaatctaa taatttagat
     3301 aaatgactgc tcgccgttga agctgatata ttcgccacag aacttagctc agtggccgtc
     3361 caagctcgcc catccatcaa agcactgagt attttaactc gtgaaatgtc agacatagcc
     3421 gcccctatcg cggctattga ggactcaaag gtaacctctt ttcgtattaa attagccatc
     3481 gcaagttcac tttattgccc aagggagcgt aacagatgca gccatactat cattgtccgt
     3541 tattaatatc agttggttag catggtcact gtattgcact aaaatattaa tgttattctc
     3601 cgccaatact cgtgctattt cgccaagttc ccccggtttt tcctgtttta acttacgaat
     3661 taatggtgtc cggatcgcaa gtactaacag tccagcttgc tctagcgcta ttttagcttc
     3721 ctttccttgt tcaacaagaa aatgagcatg gcattcatca ccaaccgtaa atatccctcc
     3781 cccttccaat ccaatacctt tattacctaa tgtttttcct agtaatgcta attgtcctat
     3841 ttgattatct aaaacaacgt gaacatcaaa cattatcctt gtccttttta aatgaatatt
     3901 cgcgagtaat atgagcaata ctaattttgt aaaaatcaaa tattgactct cgcccttctt
     3961 gttgagcttt cgcatgtaaa acatgatttt tccattgcag aactgcgtat tcgttttccc
     4021 accaagatag cgataacatt tttccttctg tagctagact ttgaaaacgt tcaattgaaa
     4081 taaaaccagc tacatgactt aatagtggt
//
LOCUS       DAJTYF010000212         4093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTYF010000212 DAJTYF010000000
VERSION     DAJTYF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.215"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..159)
                     /locus_tag="OO590_004063"
     CDS             complement(<1..159)
                     /locus_tag="OO590_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639199.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKK"
     gene            complement(404..934)
                     /gene="cybB"
                     /locus_tag="OO590_004064"
     CDS             complement(404..934)
                     /gene="cybB"
                     /locus_tag="OO590_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6639200.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1123..2124
                     /gene="gap"
                     /locus_tag="OO590_004065"
     CDS             1123..2124
                     /gene="gap"
                     /locus_tag="OO590_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6639201.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDNTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVMQLIRTLEKFAKL"
     gene            complement(2166..3605)
                     /gene="aldA"
                     /locus_tag="OO590_004066"
     CDS             complement(2166..3605)
                     /gene="aldA"
                     /locus_tag="OO590_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6639202.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3802..>4093)
                     /gene="ydcF"
                     /locus_tag="OO590_004067"
     CDS             complement(3802..>4093)
                     /gene="ydcF"
                     /locus_tag="OO590_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF218 domain-containing protein"
                     /protein_id="HCR6639203.1"
                     /translation="PDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTG
                     ELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
BASE COUNT         1100 a         1023 c          928 g         1042 t
ORIGIN      
        1 ttttttcgat gcactaatag aaccatcatt acagacaaat ttgccgcctg ccgtacagtg
       61 tgagatcccc cctttcgaac cagaacaggg tttgcgcccg gcatcggcat aaaaggaaac
      121 aagcgttccc ataaacaaaa taagtgccag ttttttcata taacctccat ggtttatcga
      181 atcttatgtg cgatttgaac gttatacgct ggtttatttt gataccgttg atgagatcaa
      241 agatcgggtg aattgattaa aaataagcca gatggacgta tgggcaagga ttatggtttt
      301 atttgtcata caaataaata tgataggcgc ttccgatgta gacccgtatt cttcgcctgt
      361 accacgggtc ggttttagta caggcgtttt ctttaaatat ccttcaggaa cgtttacgcg
      421 gcatcatgcg tagaagtgtg ttgtccttcc agaaatagtg gtgtgccagt gcagccgcag
      481 cgtgcaaccc gatgacaaaa tatcccagat tcgccagcgt ctcatgccac gactttaagc
      541 tatcaacccg ttcgaaattg gcctctgaag cgtaaggcat cgtcaaacca aacgcaaacc
      601 acgggttgcc ccggttatac atcatcacca aaccaatcac tggcagcgca ataaacagga
      661 gataaatcac caaatgtccc aaatgcgcca gtcccgtcat catcggtttc ggcttaggta
      721 taatcggcgg ggttgggtat ttcagtctta acaaaagacg aaccaccatc agcacgagaa
      781 ttgagatgcc acaggaaaca tgaatcatgt tgatgagcgg ccgatcgcta cgtgggaaga
      841 aaccacgaaa ctccattgcg caatacgctg cgataaccag caaaaagacc agccagtgaa
      901 tgctgatttg taaccttgag tatttatttt ccataacatt tcctgcttta acataatttt
      961 ccgttaacat aacgggcttt tctcaaaatt tcattaaata ttgttcaccc gttttcagta
     1021 cgttacgttt cactgtaaga atggagattg cccatcccac aatcctggtc taagcctgga
     1081 aaggatcaat tttcatccga acgttcctga caggagaaaa ccatgagtaa agttggtatt
     1141 aacggttttg gtcgtatcgg tcgactggtg ttgcgtcgtt tacttgaagt caaaagcaac
     1201 atagacgttg tcgctattaa tgatcttact tccccaaaaa ttctcgccta tctgctgaaa
     1261 catgattcaa actatggtcc gttcccctgg agcgttgatt ttacggaaga ttcacttatc
     1321 gttgatggga aaagtatcgc ggtttacgcc gaaaaagagg ctaaaaatat tccgtggaaa
     1381 gcgaaagggg cagaaatcat tgtcgaatgt actggctttt atacctccgc cgataaatcg
     1441 caggcgcatc ttgatgctgg tgcgaagaag gtgttgattt ccgctcctgc cggtgaaatg
     1501 aaaaccatcg tttataacgt taatgacaac actctggatg gcaacgacac cattgtttcc
     1561 gtggcgtcat gcaccactaa ctgccttgcg ccgatggcca aagccttgca tgacagtttc
     1621 gggatagaag tcggcacgat gacgaccatt catgcctata ctggcaccca gtcactggtg
     1681 gatggcccgc gtggtaaaga tttacgtgct tcacgcgcag cggcagaaaa tatcattccc
     1741 cacactacgg gggcggcaaa agccattggt ctggtgatcc cggaactgag cggcaaactg
     1801 aaaggtcatg cgcaacgcgt gccggtgaaa acaggttcgg tcactgagct ggtgtccatt
     1861 ctcggaaaaa aagtgactgc cgaagaggtg aataacgcac ttaaacaagc aaccaccaat
     1921 aacgagtcat ttggttatac cgatgaagaa atagtctctt ccgatatcat tggcagccat
     1981 ttcggttcag tgtttgatgc cacgcaaacg gaaattaccg ccgtgggcga tttacaactg
     2041 gtgaaaacgg tcgcctggta cgataacgaa tatggcttcg tcatgcagct tattcgcacc
     2101 ctcgaaaaat tcgctaaact ctgacgcgca caggcggagg aaaaaacctc cgcctctttc
     2161 actcattaag actgtaaata aaccacctgg gtctgcagat attcatgcag gccatgttta
     2221 ccatctgcgc cgccaatacc ggatttacgc catccggcgt ggaaaccttg catagcttcg
     2281 aagttttcac ggttgatgta agtttcacca aacttcagcc ctttaatggc tttcatcgcg
     2341 acgttcagat tttgggtata gattgatgag gtcaggccgt aatcactgtc attagccatt
     2401 gagatagcat cttccagcgt gtcaaatgcg acaactggca gcaccgggcc aaaggtttcc
     2461 tcatgcataa tcgacatttc ctggcgaaca tccagcagca atgtcggcgg ataataatat
     2521 cctttcccct ctaccgcttt gccacccaac gccactctcg ccccttcttc tactgcgcgc
     2581 gccacttttt gctcaaccct ttccagcgcc gcggcgttaa tcaacggccc catcgcaatg
     2641 tcgttgcgtt cagcggggtt accaaattga accgcctgca acgcttcacc cagccgattg
     2701 acgaactgat cataaatgcc tttctgtaca taaacacgtt ctgcacagtt acacacttgc
     2761 ccactattaa tgacgcgtga atcaacgatg gctttgactg ccagttcaag atcggcatcg
     2821 tccattacga tagccggtgc tttaccaccc aattccagac acactttggt gatgtttttc
     2881 gccgcagtcg ccatgatctt ctcacctgca gagacgctgc ctgtcatact gaccattgcg
     2941 acctttgggt tacccgccag ttcttgccca acggtttcac cacgccccag tacaaggtta
     3001 aacacgccgc gcggaaggcc tatttcatcg acgattttgg cgaatgcaat cgcattgttt
     3061 ggcgtaaatt cactgggttt aatgacgatg gtattaccgg tcaaaagagc gggagccatt
     3121 ttgcgggcaa tgaggaagaa cggaaagttc cacggcagaa tgccggtagt cacaccaagc
     3181 gcacgtttaa acagaagaat attttctcct ggacgatcgc tttgaataat ctcgccctcg
     3241 taacgccgtg cccactccgc catgtaatcg atatagtcgg cagtaaaagc cacttcgact
     3301 tcagctagct gctggatctt gcccccttct tcaacaatca gcgcactgat ttcactggcg
     3361 cgttcgcgga tcccggcgga gattttgcac aaccaactgg cgcgttcaat agcaggcaac
     3421 gcttcccatt ctggttgtgc acgttctgct gcatcgattg ccttacgggc atcctcggcc
     3481 tgaccatcgg gtatgcggga aatgacagcc tctgtagcag ggtttaccac atcaatccat
     3541 gcgtctccac gccaggtaac aaactgtcca tcgatataca taggatgttg aacgggtact
     3601 gacatgggcg actcctgtga tttatatgtt tgttttcggt gaatatattg ttaattaaca
     3661 tcaatcatca gagagacggc atcgggaaag gacaattctg tggagggctt cataaaaaca
     3721 agtgtaaaag gaataaaagg cagaaataaa agtcaggagt catgcccgtt tgcaaacaag
     3781 tggttcacaa acgggcaatt tttaacgtaa cgagcgactt tccatcgcct cgatgagcac
     3841 cgcatcatgt ttcagcgttt gccatgcatg gatgacttct gccggaaaat caacgtgaac
     3901 gataaaatct cgcccgcggg gaccgtagcc atcgctatca tcgtgtaaac gcggcagctc
     3961 gccagtgagt agtgagagat aacgctcaac cggccataat ccttgtaact gattaataaa
     4021 gattacactg tctgcgttat ttcctaactg aggaacgaat ccgggataac ttaaccagcg
     4081 tggtgcatcg gga
//
LOCUS       DAJTYF010000213         4058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTYF010000213 DAJTYF010000000
VERSION     DAJTYF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.216"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(1..394)
                     /locus_tag="OO590_004068"
                     /pseudo
     CDS             complement(1..394)
                     /locus_tag="OO590_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1084..2793)
                     /locus_tag="OO590_004069"
     CDS             complement(1084..2793)
                     /locus_tag="OO590_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6639204.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(3225..3944)
                     /locus_tag="OO590_004070"
     CDS             complement(3225..3944)
                     /locus_tag="OO590_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6639205.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1171 a          837 c          745 g         1305 t
ORIGIN      
        1 gcatgctgta cccggtcgta ataaccgctc ctggatacac caggtacccg acacatcgct
       61 tcaacaggga attgttgtcg ccattgttcg actaacgcgt atttttcagc gactcctggg
      121 caaaatacgc tgttgctttt tttaatatat ctcgctcaag tcgtgcttca tttaaagcct
      181 tacgcagttg cataacttca gattccagct cagccactgt gcgggagcct ggtgtattga
      241 gcccttttct tgcggcggta acccattgtc ctaaagtgcc ttcaggaaga gataatcggg
      301 aagcgccttc actgatcgaa agctgatttt caggaaccgt tttgacggct tcggctttaa
      361 actccgtaga gtaacgttgg ttttttctgc tcattattgg ctccttctga tgccattcta
      421 tttcaggaag gagtgtccgt taaactcagg ctacctcaga taagcttgat acaactggtc
      481 tggtatatac agtttctttg agtgcggtta cgtccacaga ggtaacggta aaagtaaccc
      541 cttgtcggga ccactccgac cgcgcacaga ggcattttca tcgctgacag gccgcattgc
      601 ctcgcagggc tggaggagga tcggtgatat ccggacgacc gtaccggctt acgccgggct
      661 tcggcggttt tactcctctg ccatgtgcaa caacggagtg ccgccttaca tgccgctggc
      721 gcggcatatc ctggtaacat ttttgttgat accaccttcc ggccttatcc tgaccagtgg
      781 gctttcctgg catcgatgat accgatgaac gcgcatgata ttgagccgac gatattacgg
      841 gcaactggaa atactcactc tctggatgtg acctttatcc atgaagaaga tttggccaca
      901 ccatggaaac ctgaacaatc atcagtttac gcccatgtaa acccctacgg gatatttgaa
      961 ctcgatatgg aaacaaggtt gccaatcgag gtggttgcct gaaagttggc agttacccgg
     1021 tcactacacc ttgttggagt gaacatatca aaaacaggta attacacaat ttaaattaca
     1081 gtctcataaa aaatgattaa atctatttat tatttcaata aacgtttttt tcatcttctt
     1141 tgaaaattgt tctgctatag tatccggttc cagattgttc tcttttatta ttctacaata
     1201 ctctaatatt gtttctttgt ctctatttac tacagataca aatacaccag gaataccatc
     1261 tttattagtt gcggacagca tttcgaccaa tcgtcttgtg gtaaaactat agttttttag
     1321 aatattacaa aaggcacgta ttgtttcggt atggccatta taaaatgcca taaaaagtcc
     1381 tgatgtaccg ttgggatttt ttatccataa taattcatct atatattcct ggtgaggggg
     1441 gagtattttt agtatatctc catacgccat aatggccttt gtgtgcccat tttgaagtgc
     1501 cataaacaac ccatgcttta ctttcaataa ttctttaatt tgttcactac taagcatttt
     1561 cttattaagc agacttccga acatacgaat actatctgca tgtccattct gcaatgcaaa
     1621 aaataaccca ggtaaatcat ggctgtcttt cgcagctaat agatataatc tcttatcgga
     1681 agttaacggg gttttaaaga ctatttcccc ataggctttt attgtgtcta catgtccgtt
     1741 cataaaagac atgcataaac ccggcacatt gtttgcccct accgcagcaa gcaatgtaag
     1801 tattgtttct tgactaagat tgcacatatt taatatctta ccatatactt gaataatgtc
     1861 tgaatatccg ttttgaagtg ctatataaaa tgcaggagta ccatcagagc ttctcgtggc
     1921 cagaaggttc accagtttag attgagaaat ttcaccattt ttgtattttt ccatcagtat
     1981 aaataatact tctgtcagat tctttgttat cgcatgatat ataactctgg aattgatcaa
     2041 ttcatctggt aaaagtttca ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat
     2101 tacctgcccg ggttgctcca gaccataaat tttccaataa aaatcatcaa aaaaatcctt
     2161 agcggataat tgactcagtt cgaaatggct atcacaagta aatacagtac gtttatgagt
     2221 aacagttcga ttaggatcga aaaactgaac aacccatttt tcactttctt gtacgcgctt
     2281 tattttcaac cctaacgcca tttggtggtt taatgtgcat gctgataatg ttctgacaaa
     2341 gtctccattt tttttcatgt tataaaaaaa attagtaata actgttcccc actttttatt
     2401 ttccacaaaa taaatataag ctggtgattt tgaaaaatct tccgtttttt ccaaacatac
     2461 attttttgca atagcatcag gggtgctaag aagctgatag tttaccttcc catttgtttc
     2521 cataaaattc atttcccaat aggaagctag gtgacggcaa actatttgtc ttccatatat
     2581 atcttttgcc actccattta tatttacctt cacatttctc ttttctgaaa aataaggtgc
     2641 tgttttattc ttaaactgaa ttagttcact ggagtttgcg accctcataa tagaacttgt
     2701 tattgttcta ttatctctgt taacagacat atctgttgat aacgaatttt ttgttgaaaa
     2761 tatactagat gaaaatgttt tatttaacgg catagaagac aaccatagag atgttataat
     2821 actattagat tgcagttact aatagtaaaa gcctccttac aaggaggtag cttattcaga
     2881 atgctcctgt aagatagcga gctcgtcaac gatacagatg atctgattat attgtgcaaa
     2941 aggagaacag gaaaataggc actggaaaag atgaaggcct gatgggagca ttaactaatg
     3001 caaaatccga caggttaata tcccgaaaag caatcaaacg actcaaacag gagatgcacg
     3061 ctgaagtggt ggacaactgt tcggatttca gatgagtagc tcagcagttt atgagccgaa
     3121 tgctctgtca cgggcaggaa tcaggctgca tacgcagtgc caaatctaat cgtagttatt
     3181 ctaaccagtt ttcaagctac aaggtggtgt agctggcatc ttctctactc tatcatcaaa
     3241 cgataaaatg gttcctctcg taacatttgc tcttgccttt cacttccatc tcgatcgctt
     3301 cgtagttcat tacgatagct gacatatttc cagtcttcag gtctaacatc tgacgccgga
     3361 tactccccgg gagcgatttt gcttcttaat agattagact cgagacacat tataaattgt
     3421 cgtattttat gaagtaataa agcactatat tgcgagcatt cctgatctga ttttacatat
     3481 agcgtaaact gagctcctat ccccacacga gattgctgag aaactcgatt catatcagtt
     3541 atcttccatt tatctatagg actgtcctct gaaaataata aaccagacaa aatttggaat
     3601 gccagaggaa cttgctccct agctatacta atatgaaact tgtccccaac aaaatccccc
     3661 ttactctgag gattttcacg acatgcatga ataaacacat ctccactatg gttcacaatc
     3721 tggaatcctt ggtacatgac agaaaaaaat tgacatatat cagagtaagt tggcacgttc
     3781 gatgctactt tgaaatagta cgctgggtat tgccgtattt ggttgtacaa tatattgaag
     3841 ttatttttca atctttcatt cgcagaaagc atctgcggta tttcagacct cacaacattc
     3901 aaagaatcta aatttagttt tagacagggc ttttttatgg gcatagaaaa cgtcctctat
     3961 aaaatagata tattatctta tattgattgt ttatcatctt ttataaaacg atacattata
     4021 tgtaatgtat gtaagcgtaa gggtcttctc cgatcatg
//
LOCUS       DAJTYF010000214         4018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTYF010000214 DAJTYF010000000
VERSION     DAJTYF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.217"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(257..1114)
                     /gene="sitD"
                     /locus_tag="OO590_004071"
     CDS             complement(257..1114)
                     /gene="sitD"
                     /locus_tag="OO590_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6639206.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1111..1968)
                     /gene="sitC"
                     /locus_tag="OO590_004072"
     CDS             complement(1111..1968)
                     /gene="sitC"
                     /locus_tag="OO590_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6639207.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1965..2792)
                     /gene="sitB"
                     /locus_tag="OO590_004073"
     CDS             complement(1965..2792)
                     /gene="sitB"
                     /locus_tag="OO590_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6639208.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2792..3706)
                     /gene="sitA"
                     /locus_tag="OO590_004074"
     CDS             complement(2792..3706)
                     /gene="sitA"
                     /locus_tag="OO590_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6639209.1"
                     /translation="MHSIKKVTMLLGCLALTCSITFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1095 a          984 c          976 g          963 t
ORIGIN      
        1 aagagctgaa tagtctgaag gatttgaatt cggttaggaa ttttatcgtg gaataacaga
       61 agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc tggtaaactt tcgatagtgc
      121 gagtattgaa tgatttctag gcgttatcga ttttacgtat tttttgcatg atagaattcg
      181 tacctcctcc caacgacctt ccatggcttt ccggacttct gtaggctggc ggacgatgct
      241 gtcctcggat gttgtgctaa ttgggtgata acagatcctg tgtatgggcg tttccctttg
      301 tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa cacgaccgcg aataagacga
      361 caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag ccagacacca gtcattgaca
      421 ctattaccga tatgccagtc gccagtagga gcgcaatatg aaagcgttgt gtcaggagta
      481 ctgcaatggc gccaggcgca atgagcagag aaatagagag aatgatacct actgctttca
      541 gcgtcgccac aatggtcagg gaaaccatgc acagcagccc ataatgcagc catctcgtat
      601 gcaaaccgct tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa tcccgccatt
      661 ttacgctaat aacaagtgta accagcccgg caataatcac cgtctggatt atatcgccga
      721 tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct ggtttaattg
      781 caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc ataactgtat
      841 cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcgcag aatactccgg
      901 cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg atgccaggaa
      961 aaaccgcatg actcatcgcg tcacccatca gtgcccaacc tttcaggacc agaaagaccg
     1021 acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga ctcataaagg
     1081 tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg ctttatgcgc
     1141 acgccgacga tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta aaagaaacag
     1201 cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt aactgaccca
     1261 cgcgccgagg aaactggtta cactgccaat agttacagca attatgagca ggcggggaaa
     1321 acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga tcaccagaaa
     1381 ggcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga agaagaggat
     1441 ttttaatctg cccggatgta atccaatagc acgggcgtga ttctcgtcga aaaaggtcac
     1501 cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa tgatagtgag
     1561 ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg tctgaatgtt
     1621 cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg acgagaagat
     1681 caggccgatg atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca gcatgctgcc
     1741 cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca gcatatatgc
     1801 ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg accagccttt
     1861 tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accattgccg agacccacat
     1921 cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct tgcttccctc
     1981 tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat aatgctttct
     2041 tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc caggttctcg
     2101 gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac catgaccgta
     2161 taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat cgttttgcct
     2221 tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac atcaacgccg
     2281 gtaaaaggtt catccagcag gatcacatca ccctgctgtg caatcgctct cgccagaaag
     2341 acgcgtttct tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa atccaccata
     2401 tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc tatacgcaac
     2461 atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac aggaaatgac
     2521 cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag cgcctgtcgc
     2581 gtgggaatac ccagaacaga tatctttccg ctggtcagac gcacaaatcc cataatcgct
     2641 ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat agatccactt
     2701 ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt gacagtaaca
     2761 tcattaacga caatgcctgc agattgcatc attatttctc ccttttcccg gctttaattc
     2821 cctgtaccag cgtactggta gtaaccttaa gaaggtcgat atacgtaggt accgggccgt
     2881 tttctgtgct cagggaatcg acatagagca caccgccgta gtgtgcgccg gtttcacgcg
     2941 caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc gggatatgat
     3001 ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt tgatcggcat
     3061 taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa gaaaaagccc
     3121 cttcactggt gaccatccat cgctgattct cagggagttc cgtaatctgc ttacgcaggg
     3181 gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg taggtttgtg
     3241 catttgccgg atcgtatttt atcagcgcat cacgaatatt atcgacgtaa atcagagcat
     3301 tatctggcga catccatgca tggggattag gtttgccctc atagggtcct tcggtgatcc
     3361 ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga tgctggtaaa
     3421 agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt gccccctgcg
     3481 cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca ggcttggtta
     3541 tggatgagac ttctgcagca tctccagcca cgtttttggc catatccgcg atgatggtaa
     3601 atgttgtaat gaccttgaat ttttcagttg cacttgcctg aaatgtgatc gagcaggtga
     3661 gggcgaggca ccccaagagc atggttactt tttttattga gtgcataatt tagtacctat
     3721 agtgagcaaa attagaacaa acatagcaac ggctatgttt tatagcataa cctatacaaa
     3781 atgaaaataa ttatcattaa cagctttgag gcctaaaaca gggattaggt tgtttcctgc
     3841 tggcgacatg atattgagga aaaaagtaga gcagctatta ggctttgaaa tgactttatg
     3901 aggaaaattg ttgatggcaa aaatggggca aaacgctgta taaggggcaa aaaaggggca
     3961 aaaaagagtg ggttatcgta gcttattgtt gtcgctgata atgtttaaca tattgaaa
//
LOCUS       DAJTYF010000215         3931 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTYF010000215 DAJTYF010000000
VERSION     DAJTYF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.218"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(180..671)
                     /locus_tag="OO590_004075"
     CDS             complement(180..671)
                     /locus_tag="OO590_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639210.1"
                     /translation="MLFNNQDWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRVEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(992..1720)
                     /locus_tag="OO590_004076"
     CDS             complement(992..1720)
                     /locus_tag="OO590_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6639211.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKMRGILIA
                     FTGTDQQKQEHLLKLAQSGKVEGI"
     gene            complement(2683..3336)
                     /locus_tag="OO590_004077"
     CDS             complement(2683..3336)
                     /locus_tag="OO590_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HCR6639212.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
     gene            complement(3329..>3931)
                     /locus_tag="OO590_004078"
     CDS             complement(3329..>3931)
                     /locus_tag="OO590_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6639213.1"
                     /translation="AKAYSQKNAGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFISLVEDRRKIEGDNAM
                     VFDGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVDRLVNVMAGVYKETGVNG"
BASE COUNT          937 a          995 c          984 g         1015 t
ORIGIN      
        1 gcgttagtag gcagaccttc attgacctga atgttgtcgg tgtcggtgat acgtactggc
       61 tcaataccga actggaaagc gtctttaatc gtcataataa tcaatctcct aaattagttt
      121 aaatactgtg agtcctcaaa actgaggagt ccctagcatc gggtcaggga ctgtgtgttt
      181 tacaggctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgggag
      241 cggccagcgg aatgggtagc actgcggagg ttttcaccat aggtccggcg ttcagctttg
      301 acctgttcgc ggcgctgtag ctctttctgc cattcgtctt cggtcatgaa catttcgcgg
      361 gtacgctctt tctgagacgc cagctcttta gcgatacgct cagcctcgac acgcttattc
      421 atctggcggc gttggtaggc ggtcatcagg tcattactga agaagttacc aatggtcacg
      481 ccagcttcag ccagagcttc cagtaactcc attgcctcgc ggctggtaga cggcaggcct
      541 gacaggtagc ccggaatgag ggtcttgata gcgagcgaca gcggataggg ctgaccgtcc
      601 agactcacag tattctcgta gagattgatg tcggtgactg acagtttcca gtcttggtta
      661 ttgaataaca tagtaattac tcctcttgag agttgagact atcggtcgac cacaggcgtg
      721 ttcagtcctg cggcgtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtattga
      781 gtagacgcgc tactaacggt gtgctgacga cagctagcaa gctagcgaca gtttcatagc
      841 gaccagttgg gctattagct cgtctcggga ttggtgctgg cgggtctcat cttccgggtc
      901 tagaccaagc tctctgagga ggacttcagt ccggtagcca cggtaacgga gagtcatgcg
      961 cccctcaggt gtgcgcgggt caatcatcat cttagattcc ctccacctta ccggattgtg
     1021 ccagcttcag gaggtgctct tgcttctgct ggtcggtgcc agtgaatgca ataaggatgc
     1081 ctcgcatctt gtcaaccatc tcaggcttac cgaagacctc cagcccctta gcgttgaacc
     1141 ggaagttata cccacctgct gagaacttct ggtctgacgg cttaatgata cgaaagcagt
     1201 cggaatactt cggatttaca gaccactcgt tactgattgg gttcttacga aagaggccac
     1261 gatgctggag aagtgcggtc attagcttct gagcgggcag accataggtt ttgcagtagt
     1321 cgccccaagt cacgcctcgg cggttagcgg actgaatggc tgctcgcttc ttctggtctt
     1381 ccagttgaag gactcgacgc tctaacgtct gaatatggtc gtaacacttg gcgcggaggt
     1441 gaacggaata gccagcgacc agaatgaaag tatgcttacg gtcgagacga tacagcggct
     1501 gctgtttccc ctgcgcgttt agatagacat cttcagtcac cccgatatga ctgctcagtt
     1561 ttgagcactg ctcagttttg agcgcgttaa gctcctcagc catattgcga atatcacgca
     1621 ggacatttcg gtgctccttg cctgtcagct cagcaatttc aactgaggtc atggagacaa
     1681 gctggtcgag gttaatgacg ctgatttctt gttgggacat ttacgtgtac tccttttgtt
     1741 tgcaccctga agtcgcaccc tactgaccta ctcaagcccc cacgtaaagg gagaccagca
     1801 ggcaacagga cgcaaacaga atgacttgag gtaattgtga tgtggtctgc gatgaccagt
     1861 agtgataaag gtgcaagaca gcatgtccga cgtccagatg gtgagcggcg atagacccaa
     1921 gtcaaaatga agacacgaga acgctaacgg gctgggagtc tgacagaggc tgtcttgcta
     1981 gccttggtat ggagaaagac ttaatgtcgg ggggcttccg cataacttga cgcatcaaga
     2041 ccagaggcgg aatgtatggc ccctatagtg atagctaagg gtccattccc tatagtgagg
     2101 gtataagatt tgctacctga taaggccata atctgacctc cagttagacc cgaagtatgg
     2161 agaccacatc atagcgacca tagtttactt cgggatgaca ggaggctgga cttaaagttc
     2221 ttcttattgg aattgtgata aacacaatcc ataacaagat aaactcatgg tctcctccag
     2281 cctgctggtc aggactcaag gttcaacctc agacaccact ggtctaacct ccttgagttt
     2341 ccgcctgtcc acaatggatg gcagacctca gcaatgatgt cttggttctt cacaatctcg
     2401 cacaccgatg gtctcataga cggaagttcc gctgaggtct gatgttctcc gcctgtcaaa
     2461 ggagagagat tgctacgtta aagggaatgg tctcaggcca aggtgattta acactaggcg
     2521 tctgcggatg gtttccaatc ggtccgctac cggaagcctg agacttaatg aatgtgtgtg
     2581 ggtcgtgaat gttattaagg tggggactta gggtctctcc cctatagtga tagttaagcc
     2641 cccttcccta tagtgagggt atattcaatc tgacctaaag ctttactcgc tagctgcctg
     2701 ctgctgttcc ctcgccttcc tgtcccggtc ctcaatgccc ttgagcgccc tttccggcat
     2761 gctgttaaac atggcttcga cttctttcgg tctggtaatc tgctcagtga caataatgtt
     2821 cagcagctta aaaagaccat gcgctaactg agggtcgtca tcaatattaa tttcacccgg
     2881 atggaccgcc tgattgccaa cgatacgaca aatatcagca gctcgctgga ccaatggaga
     2941 caatcctttg gctacaagcg acttaatgtc cgtgtcgata tgcttccccg gctcaccgag
     3001 atggaccatc agcttctgaa gacttaagcg caggagtgcg gcagcgcctt taggagaaag
     3061 gttaacaatg cttctggcct ccatgtagtc ggacttacag tcgtctggca tgtctgggtt
     3121 aggcatctca acgtttgctg acgctggtat aatcatggtt tcgtcatacc aataagcttt
     3181 ctcattgcat cgttcacatt tagaaaaggc gaaaggtgtc tcataaatcc cgttagcgcc
     3241 atattttaag cggacccagc gctgttgaga ataagcgcca caattcgggc agttaaaagc
     3301 agtgccacca aactctgccg gataatactc agccatttac tccagtctcc ttataaacac
     3361 ccgccatcac attaaccaat ctatctacct cgacctcttt cgcgtaacgg tcgtaagtta
     3421 tagacccaga gctatgaccc ataatcgcct gagcatacgc caattcgacc ccatgttgtt
     3481 ttagctgggt cgcaactgta tgtctaaacg agtgaaaagc taagccgcta cggtcagcaa
     3541 ggactttagg tagcaacgtt cggttaaacc atttgctcac ctgctcaccg tagccgttct
     3601 tcatcagctt gaggccatcg aataccatag cattatcacc ctcaatcttt ctacggtctt
     3661 ctacgagtga tataaagtca gccaatacaa aaccataagc accatcgacc agcgggacac
     3721 agcggtcact atgggcgttc ttgatactct tacccggcag gctgctatcg tcttcgttga
     3781 tatggataaa cacggtcccg gcctcagtgg tcctgacatc tttgacctga agttgggcca
     3841 cctcgttaag tcttgctcca gtgattgcag ccagagcggt gacgtaataa tggtatggct
     3901 tacctgcgtt cttctgagag tacgctttag c
//
LOCUS       DAJTYF010000216         3918 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTYF010000216 DAJTYF010000000
VERSION     DAJTYF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.219"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..324
                     /locus_tag="OO590_004079"
     CDS             <1..324
                     /locus_tag="OO590_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR6639214.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
     gene            383..586
                     /locus_tag="OO590_004080"
     CDS             383..586
                     /locus_tag="OO590_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCR6639215.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            586..1611
                     /locus_tag="OO590_004081"
     CDS             586..1611
                     /locus_tag="OO590_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6639216.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(1664..1753)
                     /locus_tag="OO590_004082"
     tRNA            complement(1664..1753)
                     /locus_tag="OO590_004082"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1717..1719),aa:Ser,seq:cga)
     gene            1847..2644
                     /gene="mtfA"
                     /locus_tag="OO590_004083"
     CDS             1847..2644
                     /gene="mtfA"
                     /locus_tag="OO590_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6639217.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2745..2820
                     /locus_tag="OO590_004084"
     tRNA            2745..2820
                     /locus_tag="OO590_004084"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2778..2780,aa:Asn,seq:gtt)
     gene            2982..>3918
                     /locus_tag="OO590_004085"
     CDS             2982..>3918
                     /locus_tag="OO590_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6639218.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGD"
BASE COUNT         1083 a          906 c          931 g          998 t
ORIGIN      
        1 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
       61 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
      121 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
      181 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
      241 aatgcacttg atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg
      301 atcccgagtc aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta
      361 gcggcggtta tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat
      421 attaacgggc tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc
      481 gttctatacc aatcgtagtg gcaaaccgat tgtcagccgg gatctattta cctgcaataa
      541 aactttacca ccacgcgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg
      601 aaaaatcctg aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta
      661 tggaaaccaa catccagaga atctgtaaca ctaaccgcca tcaaggatgg tttagctgct
      721 ttatggaaaa agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc
      781 ttgtgggaaa aattcctcgc cagcgcctat tacagtgacc tcagtccaag aacacaaaaa
      841 gattatctgc aacatcaaaa aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc
      901 ataaaaccag aacacatccg tcgatacatg gacaagagag gggaacagag taaaacgcaa
      961 gccaaccatg aaaaaagcag tatgtcccgc gtttacagtt gggggtatga gcgagggtac
     1021 gtgaaggcta acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat
     1081 gtaaccgaca aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca
     1141 atggaaattg cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggag
     1201 cagattggaa acgacgggat ctttatccag caagggaaaa caggaaaaaa acagataaaa
     1261 gcatggagtc cacgattaca ggcggcgatc gaaaaagcaa aacagttacc aacatccgcc
     1321 tatgtaatca gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg
     1381 gtagaagcaa gaaatcgcgc aggcaaaatt tcaggtattt taaccgactt cacctttcat
     1441 gatctgaagg cgaaaggaat ttcagactat gaaggaagca gtcgggataa gcaacttttc
     1501 tctggtcaca aaaccgaggg gcaagtgcta atctatgaca ggaaggttaa agtttcaccg
     1561 acacttgatg tcccgttacc tgaaaatatt ccaagaaaat attccaagta attccaagtg
     1621 tgatttttgt cactgactta atgatgtata agtgattgaa ttttggcgga gagaggggga
     1681 tttgaacccc cggtggagtt gcccccactc cggttttcga gaccggtccg ttcagccgct
     1741 ccggcatctc tccgctcaga tggttgccat gatgccagga aatttggcat tttaacagtc
     1801 cctgtccgtg caattttgtt caagtgacga gtttgcgagc aaaacgatga ttaagtggcc
     1861 ctggaaagta caagaatcag cacatcaaac tgcccttccc tggcaggaag cactatcgat
     1921 ccccctttta acgtgtctga cagaacagga acaaagcaaa ttggtcgctc ttgccgaacg
     1981 ttttttacag caaaaacggc ttgttccttt acagggcttt gagctgaatt cattaagaag
     2041 ctgccggata gcacttctat tttgcctgcc cgttctggag ttaggactgg aatggctgga
     2101 tggttttcat gaagtcttaa tttatcctgc gccatttgtg gtcgatgatg aatgggaaga
     2161 cgatatcggt ctggtgcata accaacgtat tgttcagtca ggtcagagct ggcagcaagg
     2221 gcctatcgtt ttgaactggt tggatataca agattctttt gatgcttctg gttttaacct
     2281 gattattcat gaagtcgctc ataagctgga cacccgtaac ggcgatcgcg ccagcggagt
     2341 tccctttatt tcgttgcgtg aggttgctgg ctgggaacac gatcttcatg ctgcaatgaa
     2401 caacattcag gaagaaatcg aattagttgg tgagaatgcg gcgagcattg atgcttatgc
     2461 tgccagtgat cctgctgaat gttttgccgt actttctgaa tatttcttta gcgccccaga
     2521 actttttgct cctcgtttcc cttcattgtg gcaacgtttc tgtcaatttt atcaacaaga
     2581 tcctttgcag agactgcatc acgctaatga tacagactcg ttttcggcga cgaatgttca
     2641 ttaattaaca actttgcaga ttaattaacc aattgaaatg gcttatgaaa tttagtgttg
     2701 acagacaagg taccgctaag taatatgcgc cccgttctca cgattcctct gtagttcagt
     2761 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     2821 attttctgtt ttcatgcatc cttgcgaatc tttatctgat gttgattcaa caggttagtg
     2881 tgaaaactct tctcggtgca ttttgatttt accctacgca tcgggaaaaa ttggtggtca
     2941 aatctgggat caggttagtt cgataatgga gtgaccccca tatatccctt accgacgcaa
     3001 aaatccgcac cctcaagcct tctgataaac cctttaaagt ctccgattct cacggtctgt
     3061 atctgctggt caagccgggt ggctcccgcc actggtatct caaataccgt attagcggta
     3121 aagaatcccg cattgcgctg ggtgcctatc cagccatctc cctgtctgat gcgcgacagc
     3181 aacgtgaagg tatccgtaaa atgctggcgc tgaatatcaa cccggtacag cagcgggctg
     3241 ctgaacgtgg ctcacgaaca ccggagaaag tttttaaaaa cgtggcgctg gcgtggcata
     3301 aaagtaacag gaaatggtcg cagaacaccg ccgaccgtct gcttgccagc ctgaacaatc
     3361 acatctttcc ggtcatcggg aacctacctg tatcagaact taaaccccgt catttcattg
     3421 acctgctgaa agggatcgag gaaaaaggtc tgctggaggt tgcgtcccgc acacggcagc
     3481 acctgagtaa cataatgcgc catgcggtcc atctggagtt aatcgatacg aaccctgcag
     3541 caaaccttgg cggcgtgacc acacctcctg tcagacggca ctatcctgcc ctgccgctgg
     3601 agcggctgcc tgaactgctt gaacgtattg gggcatatca tcagggccgt gaactgaccc
     3661 ggcatgccgt tctgctgatg ctgcatgtgt tcattcgctc cagtgaactg cgtttcgccc
     3721 gctggtcaga gattgatttc acaaaccgag tctggacgat acccgcgacg cgagaaccca
     3781 ttattggcgt gcgttattcc ggccgcgggg caaaaatgcg aatgccgcat atcgtccccc
     3841 tctcagaaca gtccatcgcc attctgaaac agattaagga tatcaccggt aataatgaac
     3901 tgatcttccc cggcgacc
//
LOCUS       DAJTYF010000217         3877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTYF010000217 DAJTYF010000000
VERSION     DAJTYF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.220"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..203)
                     /locus_tag="OO590_004086"
     CDS             complement(<1..203)
                     /locus_tag="OO590_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HCR6639219.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGS"
     gene            complement(214..2511)
                     /gene="xdhA"
                     /locus_tag="OO590_004087"
     CDS             complement(214..2511)
                     /gene="xdhA"
                     /locus_tag="OO590_004087"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR6639220.1"
                     /translation="MEAREATATGESCMRVDSIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATHTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASTGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAFESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            2926..3681
                     /locus_tag="OO590_004088"
     CDS             2926..3681
                     /locus_tag="OO590_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6639221.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            3760..3833
                     /locus_tag="OO590_004089"
     tRNA            3760..3833
                     /locus_tag="OO590_004089"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3792..3794,aa:Gly,seq:ccc)
BASE COUNT          971 a          957 c         1014 g          935 t
ORIGIN      
        1 gagccatctt ccgccagcgt aattccccgc agctccgcca gattatgaat atcaacaata
       61 tggcgataac ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc accggcgagc
      121 agtttggcct gcgggttgtc agccagcagg ttgatggcat cggcaagggt tgctgcgcga
      181 tggtaagaag caaaatcaaa catgatgtta tcctcaaatc aatcctgcca gatggaactc
      241 ttcatataac cgttttggcg tcagcggcag tgtattgatt gcaacaccgg tagccatctt
      301 caccgcgtta cgaatagcag cggcaacagg aattatgggt ggctcaccca gtgacttatg
      361 tccgtatgcg gattgcggct cattgatttc gacgaacgcg ctttccagtt gtggcagatc
      421 cggcatggtc ggcattttgt aatccagcag attggggtta cggaccacgc cgcttttagc
      481 atcgatgatc atctcttcaa atagcgccca gccaatgccc attcccattc cgccgtgtac
      541 ctgaccttct gccagcagtg gattaagaat atgccctgaa tcatgaacgt tgaggatgcg
      601 gttgatggtg actttgcaca gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa
      661 cgccggtggg ttagtggtgg ttttgatgga gctttcagca gagagctgcc cgccgcgttc
      721 agggtggtag aaagcgtcca tcgccaaatc ttttaacgac attaacggct cttccggtcg
      781 ttcaaccagc acgatatggc cttttatcag ggtcaaattc atcgctgact gatgtagcat
      841 gactgcggcg tgagcgatga ttttctcttt taataatagt gccgcactgc gcagcgcagg
      901 cgcggcaaca tagctctggc gtgaggcaaa tgcgccggga tcgaacggcg taacgtcggt
      961 atcttgtgtt gaaataacgc gaacgtcgct gaccggaacc cccacggttt ctgccaccat
     1021 ttgcgagaag acggtgtcgg caccctgacc gatttccgtc gcgccgcttt gcacgttgat
     1081 ggttccatcc tgattcatca gaaggcgcgc gcctgctatt tctacgccga caggccaggt
     1141 gttagaggtg tagctaaaac aggcgacgcc aacgccgcgg cgcaaattgc cttgctggtt
     1201 ctggcattct gcacggcgtt tttcccattc aaagattttc cggccttttt caagacactc
     1261 cggcaaccct gcgctgcaaa tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc
     1321 ggcgttgcgt aaacgaattt caacaggatc aatacctaac gctgtcgcgg cgtcatcaag
     1381 catagactca aaggcaaata cgacttgtgg cgcgccataa ccacgcatcg caccagccga
     1441 ggggaggttg gtatagcagg tctttgaact gtaagcgtag gcacaacgag gataaaggta
     1501 agcgacttta ttcccccccg tagaagcgat ggagtgcccg tgagatgcat aagcgccggt
     1561 gttagacaga acatccagac tataaccttt caaggttccg tcgcggttca cgcccatttg
     1621 cccgtcaata gtaaaagcgt ggcgggtatg ggttgcgagg aaacactctt cacggctaag
     1681 ggaaactttc accggaatgc cgccaagctt gctggtcagg aatgccgcca ttggctcttc
     1741 cagtacatcc tgtttattac caaaaccgcc accgacaaac ggtttgatga ctcgtacgca
     1801 tgaccaggga atatccagcg cctgaccaac cacgcggcga acaatgtgcg ggatctgggt
     1861 gctggaaacg atggtaattc gcgagtcatc ctccatccac gccagcgatg ttacgctttc
     1921 catgtgacaa tgttgaataa cgggagtctg atagtgcccc tgtacctggt agtcggcggc
     1981 atcgattgtt tgttggacat tgcccgtcga catcgtgctt tgtttcagta aattgccgcc
     2041 gttatggatt ggtgcagcgt cttctgtcag cgccgcttct ggcgtggtga taacgggtaa
     2101 ttcttgccac tcaatgctga ccaattgcgc cgctttttct gccgtgagtt catcgcgggc
     2161 cacgacgatg gcaacggcgt caccatgatg acgaacatgg cgagttagca gtgcgcgatc
     2221 ggcggtatcg cgcttgtttt cgtcaagtgt ccaggcatgc ccagctgtag cgaatggaat
     2281 atcaggcaca tcttcccaag taaaaatcgc cagtacgccc ggtaaacttc tggcttgttc
     2341 atcattaata cttacggcat aaccatgtgc gatagggcta cgtacatatt tcgcgtaaca
     2401 catgcccgcc ataacataat cgtcagtata tcgtgcccgc ccggtgacct tagcaatgga
     2461 atcgacgcgc atgcatgatt cacccgtagc ggttgcttcc cgcgcttcca tagaaatccc
     2521 ctcgatttga acatttggtt cttatggcaa agatttagtc taagcaggca agatttacgc
     2581 cagaaacgac gataagcgtt aggggaggaa ggaagtgtga tgacgttaaa aaaacacaga
     2641 tatgatacga aacaggactc tgaataggga cagtgatacg tatcaatatg agtggcgata
     2701 aaatcaatgt gataaatcag gattattacg catcaggatg ggaaataggg taaaaagtca
     2761 ggaacttcga tacctctaca gcgaatattt tgctgatgat acttcccttt gccaaatgaa
     2821 attaacctct ttctctatta ccacgttttt ccagaagcaa gagattgggt atcaagaggc
     2881 tggctgctat gatatggcgt cttgtttttt aagcgaggaa agattttgag tgcgggacgc
     2941 ctgaataaaa aatctctggg tatcgtgatg tttttatcgg ttggactgct attggcgggc
     3001 tgttcgggta gcaaatcatc cgatacagga acgtattccg gctccgttta caccgtgaaa
     3061 cggggggata cgctatatcg tatttcgcgc accacgggaa ccagcgtaaa agagctggcg
     3121 cgactgaacg gcatttcccc cccttacacc attgaagttg gtcagaaact aaaactgggt
     3181 ggggcgaaaa gtagcagtag tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca
     3241 tcggttacac cgtcatcagc ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt
     3301 tggttatggc caacgacagg gaaagttatc atgccgtatt cgacagcaga tggcggcaat
     3361 aaagggattg atatctcagc tccacggggt acacctattt acgccgcggg tgcaggaaag
     3421 gtggtgtatg tgggcaacca gctgcgtggc tacggtaatc tcatcatgat taaacacagt
     3481 gaagattaca ttacggctta cgcccataat gacacgatgc tggtaaataa tgggcaaagc
     3541 gtgaaggctg ggcaaaaaat cgccactatg gggagcacgg atgcggcatc tgttcgcctg
     3601 catttccaga ttcgttaccg tgcaacggca attgatccgc tacgttactt gccgccgcag
     3661 ggcagcaagc caaaatgctg atggcgaatt aatcagcagt cagcagcgcg gcacttgcta
     3721 aggagagcgt aaggtttata atgccttacg catctcgaag cgggcgtagt ttaatggtag
     3781 aacgagagct tcccaagctc tatacgaggg ttcgattccc ttcgcccgct ccatgacact
     3841 tcttaaagtt caataactta ctgaatcata agggata
//
LOCUS       DAJTYF010000218         3782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTYF010000218 DAJTYF010000000
VERSION     DAJTYF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.221"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            152..2149
                     /gene="betT"
                     /locus_tag="OO590_004090"
     CDS             152..2149
                     /gene="betT"
                     /locus_tag="OO590_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR6639222.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2212..3489
                     /locus_tag="OO590_004091"
     CDS             2212..3489
                     /locus_tag="OO590_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR6639223.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          920 a          863 c          949 g         1050 t
ORIGIN      
        1 aaagaacaaa tggtagtgtc cgctattgcc agtacacctc aacattccac catgcattac
       61 gattaacgcc gcatagccag ttgaactttg ctactttgtg agaggtagta ccttctatcc
      121 agtgcgaatt taattaatgg aataaatgat tatgagtgaa aatgatacaa tcccaaagaa
      181 gtctacaagt cagattaaca aagcggtatt ctttacatct gctttgctaa ttttccttct
      241 tgtcgccttt gccgccgtat tcccggatgt cgccgacaaa aattttaaac tacttcagca
      301 acaaatcttc acgaatgcca gctggttcta catccttgct gtggccctga ttttactgag
      361 tgtcacgttc cttggactct cacgctacgg tgatatcaag ctgggcccgg accatgcgca
      421 gcctgatttc agctaccact cctggtttgc gatgcttttt tcggcaggga tggggatcgg
      481 cctgatgttc tttggcgttg ccgaacctgt aatgcattat ctttcgccac ccgttggcac
      541 tccagaaacc gttgcggcag ctaaggaagc aatgcgtctg acctttttcc actggggact
      601 gcacgcatgg gcaatttatg ccattgtggc gctgattctg gcgttcttca gttaccgtca
      661 cggtctgcct ttaactctgc gctccgcact ctatcccatt attggcgatc gcatatacgg
      721 acctgtagga catgcggttg atattttcgc tgttataggc acggtctttg gcgttgcgac
      781 atcactgggt tacggtgttt tgcaggtgaa tgccggtttg aaccatcttt tcggggtgcc
      841 catcaatgaa acggtgcagg taattctgat cgtggtcatc acggggttag cgacgatttc
      901 agtggtgtcc ggtctggata agggaatacg tatcctgtct gaactcaatc tgggtctggc
      961 tttgttgctc ctggcgctgg tcctgtgtct gggaccaacc gtgcttctgc tgaagtcatt
     1021 tgtggaaaat acgggcggtt atctttcgga actggtgagt aaaacgttca acctttacgc
     1081 gtatgagccc aagtcgagca actggctggg gggctggaca ttactgtact ggggatggtg
     1141 gctttcatgg tcgccgtttg tggggatgtt catcgcacgg gtctcccgcg ggcgaaccat
     1201 tcgcgagttt gtcaccggcg tgctgtttgt tcccgcgggt tttacgctaa tgtggatgac
     1261 ggtgtttggt aacagcgcga tctatctcat tatgaaccag ggggccacag acctcgccaa
     1321 tactgttcag caggatgtgg cgctggccct gtttaatttc ctggagcatt tcccgttctc
     1381 ttctgtgctg tcattcattg caatggcgat ggtcatcgtc ttctttgtaa cgtctgctga
     1441 ttcgggggca atggttgtgg atactctggc atcaggtgga gtggcaaaca cacccgtctg
     1501 gcagcgaata ttctgggcct cgctcatggg cattgttgca attgcgcttc tccttgccgg
     1561 agggctaagt gcgctgcaaa cggtgacaat agcgagtgca ttgcccttct cagtgatctt
     1621 actaatatct atatacggac ttttaaaagc tttgcgccgg gatttgacca agcgtgaaag
     1681 cctgagcatg gcgacaattg ctcctacggc tgcacgtaac ccaattcctt ggcagagaag
     1741 gttacgcaat atcgcgtatc tgccgaagcg atctcttgtg aaacgtttta tggacgacgt
     1801 tatccagcct gccatgacgc tggttcagga ggaactgaac aagcagggga cgataagcca
     1861 cattagtgat gcagtcgacg atcgtattcg tcttgaagtc gatttgggca acgagctgaa
     1921 ttttatatat gaagtgaggc ttcgcgggta tatctcaccg accttcgcgc tcgccgcaat
     1981 ggataatgat gagcagcaga ctgaacaaca tcgatattat cgcgctgagg tttatctcaa
     2041 agaaggcggt caaaattatg atgtgatggg ctggaaccag gaacagctga ttaatgacat
     2101 actggaccag tacgaaaaac acctgcactt cctgcacctg gttcgttaat agcaacatgc
     2161 cgtcctgggg gcggcaatta ttatcccggc cgcaatatga gggaatgcag aatgatttca
     2221 cgctggaaat ggatgctgaa gcagacaatt aaaaaactat ggttcagggc aacgttattc
     2281 gcaattgtcg cgataataac ggccctttta tcaattcttt ttaaatcaat gatacctgag
     2341 tcggtttccg tgaaggttgg tgcggaagca gtcgataaca ttctgaacat actggcatcg
     2401 agtatgctgg cagtgaccac attttcgctg agtatcatgg tcacagccta cggttcagcc
     2461 actactaatg tgactcccag agctacgcgt ttagttgttg aagacgtcac cacacaaaat
     2521 gtactggcca ccttcatcgg ttcttttctc ttcagtctgg tagggattat tgccctcaat
     2581 atgggagctt atggagaaag ggggagagtc attttattca ttgtcacact ggttgtcatt
     2641 gccttaatcc tcatcacatt gcttcgctgg atacagcatt tgacctctct ggggagggtt
     2701 ggtgagacaa cggcacaagt agaacaggcg gccatcgaaa catttattgc gagagcaaga
     2761 aatccctgtc tcggtggata tccatggctt gagaacaatg aacagccgaa aggaacggtt
     2821 gcagtttatc cgaagaagat tggctatgtt gaatatattg atatggtgaa acttagcaag
     2881 ctgctgacca atgatccccg tcatgtatac ctcgtggcgc agccaggcag tttcatacat
     2941 ccgtccatgc cagttttgta cctgagtcag ggccaggagt cgtcaatcaa caccgattta
     3001 cttgagacga ttattgtctc ggatgcacgt tcatttgctc aggatcctcg attttgtctt
     3061 agcgtcatgg ccgaaatagc ctgccgggcc ctttcccctg cagtgaacga tcctggaacc
     3121 gccattgatg tcattggcag aggtgttcgt atactttcca cttacgcgca gaataaatct
     3181 gatgaaatag aagtgaaata tccttcagta catgttgcac cacttcagaa taacgatcta
     3241 ctggaagact ttttctcacc tgtcgcgcgc gatggtgcca gtatgaggga gattcagata
     3301 agagtcctta aaggactgtc gatgctgagt aaaggttggc ctgagatatt tgctgaagcc
     3361 gcacataccc tggcatttga aacattagag catgcaactc gtgctgacca tatagattct
     3421 gatagatatc taataaaatc aacttattat aatttattta gtggcgaata ttctaataaa
     3481 aaaacatagc tgcagagcaa gcaattcggt tgttggcaag tcaagggcaa agcattcgcg
     3541 tgagaatggg cgtatgcttt gtgccaaaag caaacgttgc taattctgca ctgttcagcg
     3601 atggcgcagt agagacaggc actgccactg tccagtcggc aaaagcgaac actttgccac
     3661 tgcggggata aactgattga agctctcgcg caaaatcact tattcttttg cgttaataaa
     3721 atgtaggaga tgggtcattg atcctaccca cgtaatatgg acacaggcct aagcgaggtt
     3781 ct
//
LOCUS       DAJTYF010000219         3688 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTYF010000219 DAJTYF010000000
VERSION     DAJTYF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.222"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..223
                     /locus_tag="OO590_004092"
     CDS             <1..223
                     /locus_tag="OO590_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6639224.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            269..478
                     /gene="hha"
                     /locus_tag="OO590_004093"
     CDS             269..478
                     /gene="hha"
                     /locus_tag="OO590_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6639225.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            517..1107
                     /locus_tag="OO590_004094"
     CDS             517..1107
                     /locus_tag="OO590_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6639226.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1347..1607
                     /gene="repA"
                     /locus_tag="OO590_004095"
     CDS             1347..1607
                     /gene="repA"
                     /locus_tag="OO590_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6639227.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1831..1905
                     /gene="tap"
                     /locus_tag="OO590_004096"
     CDS             1831..1905
                     /gene="tap"
                     /locus_tag="OO590_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6639228.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1898..2755
                     /gene="repA"
                     /locus_tag="OO590_004097"
     CDS             1898..2755
                     /gene="repA"
                     /locus_tag="OO590_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6639229.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3455..3646
                     /locus_tag="OO590_004098"
     CDS             3455..3646
                     /locus_tag="OO590_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639230.1"
                     /translation="MTENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          992 a          901 c          932 g          863 t
ORIGIN      
        1 ttttcagcga gatcgttatg gtcgtattct cgggcaggtt tatgcaccag acggaatgaa
       61 tattaatcag tttatggtcc gggccggggc tgcctgggta tatgagcagt acaacacaga
      121 tcccgtgcta cctgtccttc agaacgaagc cagacaacaa aaacgtgggc tctggtcaga
      181 tgctgatccg gttcccccct ggatatggag gcaccgtaaa taaagcgatg gcaatcctat
      241 cattcatctg ttctcagcgg gaataaaaat ggcaaaaaca aaacaggaat ggctgtatca
      301 gttacggcga tgttcatcag tcaatacgct ggaaaaaatt attcataaaa acagagacag
      361 tttatcaaac agtgaacgtg aatcatttaa ttcagcagca gaccaccggc tagcggaatt
      421 aatcaccgga aagttatatg acaggatacc aaaagagata tggaaatatg taaggtaata
      481 attcagcaat aattaattgc caggagcaga taaataatga agttgattat ttttattctc
      541 attgttctga ttattgcaac gctacttatc cggattatcc tcagaagtgt aaatcagcat
      601 tctcctttgc tgatgcaatt gcatgcggca ggtatacgga ctggtgatgc agagcgaata
      661 ctgtccagcg gtgaatactg gcaacgtcag aagacgctgc tgacagaaag ggaagtcagt
      721 tttatgaaag gactgttcag aattgtggat atgaagcggt ggtatctgtg tccgcaggta
      781 cgggtcgcgg atatcgtcca gctgaacggg aatatccggc cacgatcacg ccagtggtgg
      841 cagttattca ggatggtgtc tcagtggcat gttgatgtgg ttatcgttga gctgcgttcg
      901 ttcagtattg ttgcagcagt agagctggat gatgccagcc atttacgacc ggaacgcaga
      961 cgccgggata ttcttctgga agaggttctg aggcaggctg gtattccgtt gctcagaagc
     1021 cacgatgcca gaaaactgct gcagatgacc ggagaatggc tgaatacaac aggggctgat
     1081 cagcagtcac cggaacatcg tagctgacgc cttcgcgttg ctcagttgtc caaccccgga
     1141 aacgggaaaa agcaagtttt cctcgctccc ggcgtttcca taactgaaaa ccatactatt
     1201 tcacagttta aatcacatta aacgacagta atccccgttg atttgtgcgc caacacagat
     1261 cttcgccaca attctcaagt cgctgatttc aaaaaactgt agtatcctct gcgaaacgat
     1321 ccctgtttga gtattgagga ggcgagatgt cgcagacaga aaatgcagtg acttcctcat
     1381 tgagtcaaaa gcggtttgtg cgcagaggta agcctatgac tgactctgag aaacaaatgg
     1441 ccgctgttgc aagaaaacgt cttacacaca aagagataaa agtttttgtc aaaaatcctc
     1501 tgaaagatct catggttgag tactgcgaga gagaggggat aacacaggct cagttcgttg
     1561 agaaaatcat caaagatgaa ctgcagagac tggatatact aaagtaaaga ctttactttg
     1621 tggcgtagca tgctagatta ctgatcgttt aaggaatttt atggctggcc acgccataag
     1681 gtggcaggga actggttctg atgtggatgt acaggagcca gaaaagcaaa aaccccgata
     1741 atcttcttta actttggcga gtcagaaaga ttaccggggc ccacttaaac cgtatagcca
     1801 acaatcaagc tatgcgggga gtatagttat atgcccggaa aagttcaaga cttctttctg
     1861 tgctcgctcc ttctgcgcat tgtaagtgca ggatggtgtg actgatcttc aacaaactta
     1921 ttaccgccag gtaaagaacc cgaatccggt gttcacaccc cgtgaaggtg cccggacgct
     1981 tccgttctgc gggaaactga tggaaaaggc ggtgggcttc acctcccgtt ttgatttcgc
     2041 cattcatgtg gcacatgccc gttcgcttgg tctgcgtcgg cgcatgccac cggtactacg
     2101 tcgccgggcc attgatgcgc tgctgcaggg gctgtgtttt cactatgacc cactggccaa
     2161 ccgcgtccag tgttccatca ccacgctggc cattgagtgc ggactggcga cggagtctgc
     2221 tgccggaaaa ctctccatca cccgggccac ccgggccctg acgttccttg cagagctggg
     2281 actgattacc taccagacgg aatatgaccc gcttatcggg tgctacattc cgaccgatat
     2341 cacgttcaca tctgcactgt ttgctgccct cgatgtatca gaggaggcag tggccgccgc
     2401 gcgccgcagt cgtgtggaat gggaaaacag acagcgcaaa aagcaggggc tggataccct
     2461 gggtatggat gaactgatgg cgaaagcctg gcgttttgtt cgtgagcgtt tccgcagtta
     2521 ccagacagag cttaagtccc gtggaatgaa gcgtgcccgt gcgcgtcgtg atgcggacag
     2581 gcagcgtcag gatattgtca ccctggtgaa acggcaactg acgcgtgaaa tctcggaagg
     2641 gcgcttcact gccagtcgtg aggcggtaaa acgtgaagtg gagcgtcgtg tgaaggagcg
     2701 catgattctg tcacgtaacc gtaattacag ccggctggcc acagcttccc cctgaaagtg
     2761 acctcctcag aataatccgg cacgcgccgg aggcattcgc acgcctgaag cccgccagtg
     2821 cacaaaaaat cagcgctaca tacaaaaaac aacctcatca tccaccttct ggtgcatccg
     2881 gtcccccctg tttttgatac aaaacacgcc tcacagacgg ggaattttgc ttatccacat
     2941 taaactgcaa gggacttcac cgtaaggtta caaccgttca cgtcataaag cgccagccgt
     3001 caaccctaca gggtgcaatg tatcttttaa acacctgtgt atatctcctt taaactactt
     3061 aattacattc atttaaaaag aaaacctgtt cactgcctgt cctgtggaca gacagatatg
     3121 cacctcccac cgcaagcggc gggccccaac cgaagccggt ttagttacaa catgcaaaca
     3181 taaaccccta aaaaacccaa gtccaacgca gaactgaaac cacaacgccc ctcctcataa
     3241 ctgaaaagcg ccgccgcccc gcccgaaggg cgggaacaga gtcgctttta attatgaatg
     3301 ttgtaactac atcatcatcg ctgtcagtct tctggctgga cgtatcgagt acacgctcgt
     3361 aagcggccct cacggcccgc taacgcggag atacgccccg acttcgggta aaaccctcgt
     3421 cgggaccact ccgaccgcgc acagaagctc tatcatgact gaaaacgggt atggcttagc
     3481 agggctgggg atgggtaagg tgaaatctgt caatcagtac cggctgacgc cgggcttcgg
     3541 cggttttact ccggtatcac atgtaaccac agcgtgccgc cttccatgcc gctggcgcgg
     3601 catcagaata atacaagcag catttaatgc atttgcaaaa gtgtgagtaa tattaagtgg
     3661 tcgtttcagg gatatcgtta agacttga
//
LOCUS       DAJTYF010000220         3651 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTYF010000220 DAJTYF010000000
VERSION     DAJTYF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.223"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(2..298)
                     /locus_tag="OO590_004099"
     CDS             complement(2..298)
                     /locus_tag="OO590_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6639231.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            1050..1598
                     /locus_tag="OO590_004100"
     CDS             1050..1598
                     /locus_tag="OO590_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6639232.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRNMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVEALQTKGVRVVSLREGFDLL
                     TPIGRAMLTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISRATYYRLAK"
BASE COUNT         1131 a          690 c          815 g         1015 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tggtttttct actgaaatga ttatgacttc aataaccctt gaatgaagtc
      541 tattcggttt tctaggagac ctgttaaaaa caggacaata tcaggtgaat gactatgcaa
      601 tttaatagta gatttccaag tatctaagca gttttacaca aattctatgc agtatcggta
      661 tagtctcttt ttagcataac ttattgaaat atatagagaa tctgtgaagt ctcttttcag
      721 ggaactatgc agggtgttag tcgttcctct ctcgactcct agaaaagtgc tatatacagg
      781 actatataac gatatataga tgtttggtca catggtgtaa ctatcaagaa acttaacgat
      841 ttcctacgtg aaagttagaa agtgatggtt atagttaccc tcctaaaatc ttcatagctc
      901 cttcataacc agtcttaaaa ggacttcctt ttgagacact ttcaacgtaa cggtagaatg
      961 caccctcaag tagtctcaaa aggttgtttt tggattatga gacagctaaa cttatgagac
     1021 taacttttga gacttctaag gggaacatta tggcggtgat tggttattgc agggtatcaa
     1081 cacaagacca gactgtagac aaccagaaac tacagataga gaaggttcac aaggtcgata
     1141 aatggtttat tgatgaggct gtctcaggag ctacaaaggc caccagcaga gatggtttta
     1201 gaaacatgat gaactatgta aggtgtgacg atacgttagt tgtggtagct attgaccgtt
     1261 tagggcgtaa taccgttgat gtgctctcta acgtggaggc actacagact aaaggtgtga
     1321 gggttgtttc tctacgtgaa ggttttgact tattaacacc aatcggtaga gcaatgctta
     1381 ctatgatggc tggtttagct gaccttgaaa aagaccttat cagtgaacgt agaaaggctg
     1441 gtattgaacg tgctaaagct gaaggggtac atatggggag gcctgtaaaa gcttcttcag
     1501 aggctgtaca gacgcttatt tcacaaggta agacgaggtt gcagattcag gaagaattag
     1561 gaatctctag agcaacttat tacagacttg ctaaataagt tactgaatag ttacatggat
     1621 gtagctgata tttggctttt ttgcaaaggg gatcacatag ctggtgtaac aggtacttag
     1681 cagctacaga catctgtcag atatttttga agattctcag agagggaatt tgaagagggg
     1741 ctactatgat tttataaaaa ttttcagagt ctgaaaagtt actgtacaga ttttatacaa
     1801 actaaaaagg actcccgtag gtgattcagt aatgaattac ttcacgaaga gtccctttac
     1861 aagtcgattt ttaatcgtgg ctacgacaat ccacccatac cagacctaac cgtaccccac
     1921 cttaccaagc cataccctac cccgccagac tataaggaag cgtagagaac cgatgaaggt
     1981 gtctgcacag ttgcatctta aacaaccatc acagtgatgt caaatagttc ttttaagtag
     2041 gaggggttcc cctatggtca catatcccct ctctcccgct tagtgtccct tgatagtatc
     2101 tagagagtat gcctgtaatg ttagagagta tgcctgtaat gtttctgtat caggtcagct
     2161 ataagaggtc tggatattac cttgtaagat tcttggtagt gattatgcag agggcttgat
     2221 aagagtgttt aacgggagga agagaaccat gaaaccgaag gtaggttcta tcttaacagt
     2281 gactgtaaga gatacctgaa aaaatacttt aacagaacta ccaagaagag agggaacgga
     2341 agagagggaa cgataagacc agaggtagtg acctgttaaa caggctgttt caaagtctct
     2401 taaaagtctg ttaagagggc taggtgaaca atcctgccat tttgtcaagt gttttgtctt
     2461 tagcaaaaag tgctaaactt aacaaaaagc actaaatgct aaagaggcga ttaagaaata
     2521 tactaagagg ggcgcttcgc ttggggtcac tatgttcttt ctctcccgtt aagaggattt
     2581 acagggacat ttcaaagccc tgataaggta agcccgatga taacagaaac agagggcaaa
     2641 accacttatc aacactgtga acgaataacg taaagggctg gttaagaaag tcggtttagg
     2701 gattggtaac aataccaatt agaagggagc ttccctttct ggggtgtcgc tctatgggtg
     2761 aagttaacct ttgaagttct ctgtgaagaa actgaacagc tattatattg gctaattaca
     2821 accctttgaa agataagcat ttttagaagt tactgttaag gttgcagagt tgtctgtgaa
     2881 gagacttgtc agttctctat attgggtggt tacaactcat tgaaaaataa gagcttttag
     2941 aggttactgt cagagaggtg aatacttgat agatacagtc ttccggttct gtcaggctat
     3001 tttttaacag atagcacaaa atgctaaaga tgcccctatg ccatctcgta caggggggtt
     3061 ttgaagggag tttaacgggg gtctgtgtgg tcattatcct taaggtcaag cgtccatgtg
     3121 cgaccgaaca gtttaaagac aacaagcact ctgacgagga cgtaacgaag tagcagatct
     3181 gcaataagtc caatacctcc accagcccat acatcaggtg accccagctc aggagggaga
     3241 tttaccatta gttttccttg taaggcgctc tcaatcgcat tctgacacaa aaaagtacct
     3301 gaccttatga gttgacctga agaggtcatt taactcgtta cagagcgatt gaagaggtct
     3361 gtgtgcaatg caataaccat gtgaccagat acatagccac gcctcatatt ttactctccc
     3421 cataaaccca ttacaccgca gatgttacga aattgttaaa aatacttggt attagtcttt
     3481 gaaaatgcta gcattgtata tataagttaa cagaataata acatgttatg aggggcagaa
     3541 tatgaatgag agacgcagaa tttagagcga tgcttcaggc atcaagagaa cgcaacaagc
     3601 acaactccta cgcttacaca aacaacccca ccagctacga agtcccaacc t
//
LOCUS       DAJTYF010000221         3587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTYF010000221 DAJTYF010000000
VERSION     DAJTYF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.224"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            29..490
                     /locus_tag="OO590_004101"
     CDS             29..490
                     /locus_tag="OO590_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6639233.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(531..1001)
                     /locus_tag="OO590_004102"
     CDS             complement(531..1001)
                     /locus_tag="OO590_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6639234.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1048..1767)
                     /gene="yehT"
                     /locus_tag="OO590_004103"
     CDS             complement(1048..1767)
                     /gene="yehT"
                     /locus_tag="OO590_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6639235.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1764..3449)
                     /gene="btsS"
                     /locus_tag="OO590_004104"
     CDS             complement(1764..3449)
                     /gene="btsS"
                     /locus_tag="OO590_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6639236.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          952 a          925 c          818 g          892 t
ORIGIN      
        1 ttcttgatta aataaaatgg agttttacat gaaggctttc aataagctgt tttccctcgt
       61 tgttgcatct gttctggttt tctctcttgc tggctgcggt gacaaagaag aatcgaagaa
      121 attcagcgcc aatctgaacg gcactgaaat tgccattacc tatgtctaca aaggtgacaa
      181 ggtgcttaag caatcttctg aaaccaaaat tcaatttgcc tccattggtg caaccaccaa
      241 agaagatgct gccaagacac ttgagccgtt aagcgccaaa tacaaaaaca tcgcgggtgt
      301 tgaagaaaaa tcaacctata ccgataccta cgcgcaggaa aacgtgacta tcgatatgga
      361 aaaagtggat tttaaagccc tgcagggtat ttcaggaatc aacgtttctg ctgaagatgc
      421 caaaaaaggt atcactatgg cgcaaatgga actggtgatg aaagccgctg gttttaaaga
      481 agtgaaataa tctgtcggcg gccatgtttc gcatggccgc catacccgtc ttagcttttc
      541 ttcacatgct ggcgcgctgc cagtccacgc agaaaataac gtaaaaattg atcgccgcat
      601 tcgcggaagt ttttatgatc cggtgcgcgc atcatcgctg taatttccgg catcgaaacg
      661 cggaactgct gttcggtgag gatagccaga atgtcatcgg ttttcagcga aaacgcgatg
      721 cgtaattttt tcagcacgat gttgttatta atgcgacgtt ccggctccag tgccggagca
      781 gactcatcct tgccgcgttt ttcataaatc aggccattga ggaatgacga caaaacaatg
      841 tccggacaac gctgaaaacc ctcttcgtct tctttacgta gccagacggc gatctgttcc
      901 gcggtggctt cgacattatc cagcgccaga atacgcacca ggtcattatt attggctttc
      961 aaaatgtagc gcacgctgcg cagaatatcg ttacttatca tgaggccttc aggtgttgat
     1021 gaggcaaaaa gccattttag cagtctttta caggccaatc gcctctttta agcttttcag
     1081 ataacggcgg ctgaccggca cggttaagcc attacgcaaa atcaactcgg cctggccgtt
     1141 atcttccaga cgaatctcct gtaaatgcgc gaggttaacc agatactgac gatggcagcg
     1201 cagtagtggt gtacgacttt ccagggtacg taatgtcaat tcggtaaagc cctctttccc
     1261 ttcgtggctg gtaacgtaga caccgctcat ccgactgctg acaaatgcca catctttcat
     1321 ttgcagcaaa taaatccgac tatgccccgt acaagggata aatttcagcg cctgttgatt
     1381 ttccggtaac agcgaaacat cctgcttgct gcgctcctga cgcaatcgcg ccagcgtttt
     1441 ctccagtcgc gcttcatcaa ttggcttcag cagataatca aaggcatgtt cttcaaaggc
     1501 tttaatagcg tattcgtcaa acgcagtgag aaaaacaata tacgggcgat gttccgggtc
     1561 aagcatcccc accatttcca gaccactgat gcgcggcatc tggatatcga gaaacagcac
     1621 atccgggcgc agtttatgca ccgcgccgat cccttccacg gcgtttgaac actctccaac
     1681 gatttcaata tcgctctgct cctgcaaaaa tatacgcagg ttctcccgtg ctaacggttc
     1741 atcatcgaca attaagactt taatcatgcc tcgtccctcc atggtagtcg taacgttatt
     1801 cgggtgtaac tatcaggctc acaggcgacg cttattccat agtcatcgcc aaaccgttca
     1861 cgtaaacgct tatccaccag attcatcccc agcccactgg cattggttac cggttgatac
     1921 aaaccggcat tgtcttcgat ctccagcatc aaatgttgcc cctcacgtcg ggcgctgatt
     1981 gccactcgcc ctgtatccag cagttgtgat gtcccatgtt taatggcgtt ttccactatc
     2041 ggttgcaggg taaacgcggg caattgctgc tgggataatt cttgcggaat agcaatgttg
     2101 acctgcaacc gcgactggaa gcgcgccttt tcaatttgca gataagcgtt cacatgttca
     2161 atttcgtcgg cgagagtaac aaactccgaa ggccgcttta agtttttgcg gaaaaaagtg
     2221 gaaagatact gcaccagctg gctggcctgt tcgctgtcgc ggcggatcac cgctttaatg
     2281 gtgttaagcg cattaaacaa aaaatgagga ttcacctggg cgtgaagcag tttgatttct
     2341 gactgggtga gcatcgcttt ttgccgctca tattgcccgg caagaatctg cgccgaaagc
     2401 agttgcgcaa tcccctcgcc cagcgtgcgg tttattgaac tgaataaacg gtttttggct
     2461 tcatacaatt tgatggtgcc catcacgcgc tgattttcgc cacgcaacgg aattaccaga
     2521 gtcgacccca gtttgcattg cggatgcaaa gagcaacgat aaggtacttc gttgccatca
     2581 gcgtagacca cttcaccggt ttcaatcgct tttaaggtgt aagtcgaaga aatcggtttg
     2641 ccgggtaaat ggtggtcgtc accaattccg gtaaaggcca gcaatttctc tcgatcggta
     2701 atcgcgactg caccaatatc cagctcctga tacagcacct gagccacttt catgctgttc
     2761 acttcgttaa acccctgtcg caaaatgcct tccgtcgagg ctgccacttt cagcgcagtg
     2821 gcagaaaaag ccgaagtgta tttttcaaac atcgcgcgtt tatcgagcaa tatacgcata
     2881 aacagcgccg cgccgacggt attggtgacc atcattggcg cagcaatatt actcaccaga
     2941 cgcaccgcat cttcataagg tcgggcgatc gcaaggatga tcagcatttg caccatttca
     3001 gcgacgaacg tgacggcacc ggcggtaatg gggttaaaga ctttatcagt gcgcccgcga
     3061 cggatcagga tgctgtgtac caggccgccg agcaatcctt caacgatggt tgagatcatg
     3121 caacttagcg cggtcatgcc ccccatcgaa tatcgatgta agccgccggt cagaccaacc
     3181 agcccaccga cgaccggacc gccgagtaag ccgcccatta ccgcgcctat cgcacgggta
     3241 ttggcaatag aatcgtcaat gtgcaaccca aaccaggtgc ccatgatgca gaagatggaa
     3301 aagacgatgt agcagagaaa tttatgcggc agacgaaccg tgacctgcat taacggtatg
     3361 aataatggcg ttttactcat taaccacgca atgactaaaa aaacgcacat ctgctgaagc
     3421 agcagcaaca ccagattaaa atcgtacata cccgcaaacc acacttccct ttaaaacgcg
     3481 taacatacat tgcctgcgtt taactttctt tgaactcttg cagaaaaatg agaattcgtg
     3541 agtacgatca ctcaaaatcg cctggcaaaa ataaaatcac cctatag
//
LOCUS       DAJTYF010000222         3545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTYF010000222 DAJTYF010000000
VERSION     DAJTYF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.225"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..328)
                     /locus_tag="OO590_004105"
     CDS             complement(<1..328)
                     /locus_tag="OO590_004105"
                     /inference="COORDINATES: protein motif:HMM:NF013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6639237.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRD
                     YLHKVTT"
     gene            complement(461..766)
                     /locus_tag="OO590_004106"
                     /pseudo
     CDS             complement(461..766)
                     /locus_tag="OO590_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            834..1449
                     /gene="tnpA"
                     /locus_tag="OO590_004107"
                     /pseudo
     CDS             834..1449
                     /gene="tnpA"
                     /locus_tag="OO590_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1777..2859
                     /gene="ompC"
                     /locus_tag="OO590_004108"
     CDS             1777..2859
                     /gene="ompC"
                     /locus_tag="OO590_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6639238.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKVNL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3348..>3545)
                     /locus_tag="OO590_004109"
     CDS             complement(3348..>3545)
                     /locus_tag="OO590_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6639239.1"
                     /translation="SEGISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRF
                     TERFHNRFKITPRELRKASRE"
BASE COUNT         1002 a          789 c          729 g         1025 t
ORIGIN      
        1 tcgttgtgac tttgtgaagg tagtccctgc ggatatttgc gatacaggaa tgcagtcgct
       61 gtattttgcg tttctgcttc tgccagttgt tgctgaattt gaccttgcgg cttaactgtc
      121 gctgaagtct cgccagcgtc ttctggtttt tctgaaaact gtttacaggc tcaaagactg
      181 tgccatctga cagcgaggca agtttagcca cgccagcatc cagcccgacc attgatgctg
      241 aagggtgaac cggagttgat acttcacttt ctgcctgaat actgatgtac cacttaccgc
      301 aggactggct gacagtgaca tttttcacaa cacccgtgac ctgacggctg ttccggtagc
      361 gcatccagcc aagttttggc agaaaaatac ggctgttttc ctgatcgagc ttaacgccct
      421 gcggatagcg gaatgcatca ttctgtcacc gctttttgaa tcaggggaac gccgaccgct
      481 tctggaaaaa gtttttgcag gcctgctcag ggtctttcag tgactgttgc aatggctgtg
      541 acggagcatc tttaatccat tgcgtttcag tggcattttt ccactcaacc aatcaggaag
      601 ccattttcgc gtaagggatg tctttgttac cggccttatg attctcattc tgacgtgcca
      661 gagtacgact gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgcgcctgtt
      721 gaccaccggg tcttaaccgg aatttgaata cctgtagtcg tttcacaggg caaattatac
      781 tttggtctgt gaaaaaaact gatatccgac gcggaaggca ctgtgttttc ctgatgcatg
      841 ctcaccgggt ctttgttacc aagtaccgac gccagatttt tgaccatgac gcgacagaaa
      901 aactacgtac ttacttttca aatgtatgtg ctgattttga agctgaactg gttgaaatgg
      961 atggcgaacc agagcacgtc catctgttaa ttaactatcc ccccaaactg gcgatatcca
     1021 gtctggtaaa caaacagcct caaaggcgta tcgggtaggt tactgcgacg ggatcggcca
     1081 gatattgctg ccaggtatta ccgcaaagat gttttgtggc gtcccggcta ttttgccagt
     1141 agctgcggag gtgcgccaat atccgtcatc cgccaataca ttgaacaaca gcaaacacct
     1201 ggtcaggtga aaaaacgagc cttatatccc cgccctgaag gacgggcttt tacggtgcac
     1261 tggataagcc tggagattat ctacacctgc atggacatca ttgtactatg ccccgtacat
     1321 gccccatcac atcctcaaca tcaaagtatt agagccttgc aaaccattat gcgtctcagt
     1381 ttgtctcatc tatcttacgc aaaaaaaata gaactaatat caagcggcgc tatattgcgt
     1441 attaaataaa accacctcaa ttacattaat aaatctaaat aaatatcata caaataattt
     1501 ccatggttat ttttaggcta tttaaattaa aaaaataaat tatgagtcaa tcataaataa
     1561 caacctttaa tataatttga ctaccaaatt tatattcgtt cacaaaatag tcatgcattg
     1621 tgtgcaatag aaacagttca gataaaaata gggatctact tcacaattta aatgagaaac
     1681 taaaacttac atcttgaaat aatctcattg attggatgaa tatttgtagt gcagtgacat
     1741 catttttaaa taatagttca aataaaaggg ctcacgatga aaaaattaac agtggcaatt
     1801 tctgctgtag ctgcatcagt actgatggcg atgtctgctc aggcagcaga gatttacaat
     1861 aaagacagta acaagctgga tctgtacgga aaagttaatg ctaagcacta cttctcttct
     1921 aacgatgcag atgatggcga cacaacttat gttcgtctgg gcttcaaagg cgaaactcaa
     1981 atcaacgatc aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgtgct
     2041 gaatctcaag gttcttccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt
     2101 gactacggct ccatcgatta tggccgtaac tacggtgtag catacgatat cggtgcatgg
     2161 accgacgttc tgccagaatt cggtggcgat acctggaccc aaacagatgt gttcatgact
     2221 ggtcgcacca caggtgttgc aacctatcgt aacaacgact tctttggtct ggttgatggt
     2281 ctgaactttg ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaac
     2341 ggtgatggtt tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc
     2401 tatgctaaat ctgaccgcac taatgatcag gttatctacg gtaacaacag cctgaatgca
     2461 tctggtcaaa atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat
     2521 ctggctacca cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc
     2581 aacaaagcac aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg
     2641 tccgttgctt acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg
     2701 attgaatata ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt
     2761 gattacaaag tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc
     2821 gatgatatcg ttgctgtagg attggtttac cagttctaat ctgattactc aaagatatgt
     2881 tgtgggaggc tttgcctccc caacatataa gtggcgccct caagccactt tccttagaag
     2941 cactatcttg cttctgacta tataaattac cctttatttt ctgggacgtt tcaacgcccc
     3001 atttttaata atactcagtg aacaattaac gtatcaatta gatttaataa caacgatatc
     3061 cgtacctaac ctgatatcta atgtcataag catcccttta attatcccct caacctcttg
     3121 taaatttccc actcttgagc tccacatagt ccaaacgccg gatgcaaaaa aaatcacacg
     3181 tgcatctctg aaattgtgat ggaggaagtg agtatcaatt atatgtgctt ataaacgttc
     3241 ccgattggca cagaagtcac cggattgttc aggctccaat aggttgagta tgtcgtattt
     3301 attttccaaa ataaaaatcg atgctgttgc tccttaataa aatactttta ctctctggac
     3361 gcttttctta gctctcttgg tgttatctta aaacgattat ggaacctttc agtaaaacga
     3421 gaaggacact tgtaaccatt ttccctggca atctcgctga tgggttttac tgtcgtttgt
     3481 atagcagaca acgcattatt taacctcacg tcgtccaata tactttgaaa acttattccc
     3541 tcgct
//
LOCUS       DAJTYF010000223         3514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTYF010000223 DAJTYF010000000
VERSION     DAJTYF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.226"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            289..807
                     /locus_tag="OO590_004110"
     CDS             289..807
                     /locus_tag="OO590_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6639240.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     SFQGKEWVHLKE"
     gene            814..1047
                     /locus_tag="OO590_004111"
     CDS             814..1047
                     /locus_tag="OO590_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6639241.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     gene            complement(1349..1816)
                     /locus_tag="OO590_004112"
     CDS             complement(1349..1816)
                     /locus_tag="OO590_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639242.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
     gene            complement(2886..3077)
                     /locus_tag="OO590_004113"
     CDS             complement(2886..3077)
                     /locus_tag="OO590_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6639243.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            3142..3447
                     /locus_tag="OO590_004114"
                     /pseudo
     CDS             3142..3447
                     /locus_tag="OO590_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
BASE COUNT          844 a          787 c         1042 g          841 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcagggcgct gacactccct gataatctgc cggaaacgaa acaggcgctt gcagagagcg
      121 tcacacgcgg gatagacgct ctttatcatg tcggggagat aaaaggccgt caggatgtga
      181 ttcaggcgct cacagaggca gggcttgaag tggtcagggt gacgcgaagc agtatcagca
      241 ttgcagaccc gaacggtggg aagaatatca ggctgaaagg agcattctat gagcaatctt
      301 ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa
      361 atgctgaacg acgagttcag gaagctcgaa gaatctgtaa gcaaggctgt gacatcaaac
      421 gagacgaaaa tcaaagacgc tatagccctg ttcacagctt cgacagagga atcactgaaa
      481 aaacaccggg aaggggtgaa agaggcgatg atgcagcaca ggaaggacgt gttaaagctg
      541 gcagggaata cgggcatgat gttactgggg atggtcattt tcctgtttac cgtgagtggc
      601 gggacgctct ggtatcttgg cgggatgata caggcgaacc tggaagaaat caggaagcag
      661 aacgagagtc tggagaagct gaacgcgaag acatggggcg tggagtttgt gcaggacggg
      721 aacaggaaat ttctggtcct tccttacggg aagtcagcag aggtgatttc ctttcagggg
      781 aaggaatggg tacatctgaa ggagtgacac agagtgacag agctggaaac acatttgctg
      841 aacgccttag agcagctgca acaggactat atgcagcggc tgagcgaatg ggagagcgcc
      901 ttcgtggaat tgcagaaaat gttttcgctt acgcaacggg acaacgcgat gctgaacgag
      961 cgggtcatgc agttgagtca gcaggtgcag cacttgagcg agcagacaga acgcttgagc
     1021 cagttataca gcgagaactg gagataagag aggaacggct gatacaggag cgcgaacatg
     1081 cgttatccct ggaacgggag cgtcagccgg aaatacagga acgcacgctg gatggtcctt
     1141 cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg gattaggagg ggttaatgtg
     1201 gcctgtacca tctggttgca taggtattca tgcggttaaa atttatcagg cgcgatcgcg
     1261 gcagtttttc gggtggtttg ttgccatttt tatctgtctg ctgccgtgat cgcaccaaac
     1321 gcgtttcagt ggtgcgggca ataagatatt acgttaaatt gtgagagaat ggtccccata
     1381 tactgacttt atcatacgga tagggttggt agtgggtcga ttgggtttcg gcattgtttc
     1441 cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat tctcctgagc
     1501 ctggaaagtc taagtgttta gaattgatgg caaaatatct tccgtccgat tcttgaagta
     1561 gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt tgtagttgtt
     1621 gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt attattctct
     1681 cttttaataa gctgtttacc acaggattta gttgttgtct aaagcacgca gataatgcca
     1741 ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt gtttgatgtg
     1801 atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg gctataaaac
     1861 aaaattttca tagagtgatt caagatcttc ttgagatctt ttttggcggt gcgtaatctg
     1921 ctgcctgcaa acaaaaaaac caccgttatc aacggtggtt tgtttgccgg aatctagagc
     1981 taccaactct ttcttccgaa ggtaactggc ttggtggagc acagatacca aatgctgtcc
     2041 ttctagtgta gccgcagtta ggccaccact tcaagaactc ttaatatctc aatccacctt
     2101 atccagttac caatggatgc tgccagtggc gttaagtcgt gtcttaccgg gttggactca
     2161 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2221 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
     2281 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2341 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2401 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2461 ctatggaaaa acgccagcgg cgcagccttt ctttgccctt tgcctttttg ctcacatgtt
     2521 ctttccggcc ttatcccctg attctgtgga taaccgtatt accgtctttg agtgagctga
     2581 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga
     2641 gcgccggaag ctgcattttc tccttacgca tctgtgcggc atttcacatc ggctatggtg
     2701 cacttttcat accatccgca ctgatgccgc ataagtaagc ctgtgtacac tccgctatcg
     2761 ctacgtgact gggtcatggc tgcgccccga cacccgctaa aacccgctga cgcgccctga
     2821 cgggcttgtc tgctcctgac atccgctcac aggcaagctg tgaccgtctc cgggagctgc
     2881 ttgtgtcaga ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg gtaaagctcg
     2941 tcggcgtggt cgtggagcga ttcacagata tcagcctgtt catccgcgtc cagttcgttg
     3001 agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     3061 ttttcctgtt tagtcactga tgcctccgtg taagggggat ttctgtttta tggggtaatg
     3121 ataccgatga atcgcgagag gatgctcacc atacgggtta ctgatgatga acatgcccgg
     3181 ttactggaac gctgtgaggg taaacagctg gcggtatgga tgcggcgggt ctgcctgggt
     3241 gagccggttg cacgctccgg gaagctgccg acactggcac cgccgttact gcgtcagctg
     3301 gccgccattg ggaataacct gaatcagaca gcgaggaagg tgaacagcgg gcagtggtct
     3361 tccggtaacc gggttcaggt ggtggccgca ctgatggcca tcggggacga gctgcgccgg
     3421 ctgcgtctgg ccgtcaggga acaggggcgc gggatgatag ttaagtttca tgcccgggga
     3481 aaaggtggtg gcagtggtcc ggttgattac ctgc
//
LOCUS       DAJTYF010000224         3432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTYF010000224 DAJTYF010000000
VERSION     DAJTYF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.227"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            180..437
                     /locus_tag="OO590_004115"
                     /pseudo
     CDS             180..437
                     /locus_tag="OO590_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO590_004116"
     CDS             complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO590_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6639244.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHKQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO590_004117"
     CDS             complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO590_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6639245.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO590_004118"
     CDS             complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO590_004118"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6639246.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3062..3205)
                     /locus_tag="OO590_004119"
                     /pseudo
     CDS             complement(3062..3205)
                     /locus_tag="OO590_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
BASE COUNT          883 a          813 c          823 g          913 t
ORIGIN      
        1 cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat tagctaacta
       61 atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag acaacggtga
      121 aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag gattacatca
      181 tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg
      241 ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt atagaacagt
      301 tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca tcgatgattt
      361 ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt tatttccttg
      421 gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc agttattgaa
      481 gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact ggtcaggttt
      541 aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa
      601 tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg cctgtaaatg
      661 ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg tcatgttttc
      721 attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc cacgaatggc
      781 atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa ttggcgttac
      841 cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca gttcgatcgt
      901 ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg cgagattcac
      961 cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc acaccgggct
     1021 tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc
     1081 atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt cctgaatgat
     1141 ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc cagcattgag
     1201 ataatatttt gcctgcttgt ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat
     1261 agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt catagatcgg
     1321 caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc gccgcaatgc
     1381 tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca tagccataca
     1441 gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc catatctggt
     1501 gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt aatgatatga
     1561 cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga ccagcaagct
     1621 gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc agcataccgc
     1681 ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc agatagttac
     1741 caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg cgttgggcaa
     1801 aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt ttcttcgaca
     1861 acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc atcatctgct
     1921 gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga atttcacata
     1981 ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg atggcgtcaa
     2041 atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga
     2101 ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt tcatcaccag
     2161 ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata ttatcaagct
     2221 gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct
     2281 ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt tccgggatca
     2341 taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta gggagcaagt
     2401 aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata tatgtctatc
     2461 tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa caagctggca
     2521 caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc actcttttcg
     2581 ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc gatggcaagg
     2641 tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg
     2701 gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg ggatagtgcg
     2761 tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg gaagcgtatc
     2821 aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac cacctgggtt
     2881 ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca gctgcaccat
     2941 gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat cgcaaatcac
     3001 ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca tccgttctct
     3061 cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga taccacgcca
     3121 gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact aacgaatagt
     3181 aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga ttttttaatt
     3241 tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga taattccagt
     3301 cagtgaaatt ttagcgtagg ctatctgtta tttgattctg tggcgtgata gccgccagac
     3361 accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt tacagagcta
     3421 aaaaatgaat aa
//
LOCUS       DAJTYF010000225         3380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTYF010000225 DAJTYF010000000
VERSION     DAJTYF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.228"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..934)
                     /locus_tag="OO590_004120"
     CDS             complement(<1..934)
                     /locus_tag="OO590_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HCR6639247.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFIVYSFACFFIGKFIANILM"
     gene            complement(962..1882)
                     /gene="rbsK"
                     /locus_tag="OO590_004121"
     CDS             complement(962..1882)
                     /gene="rbsK"
                     /locus_tag="OO590_004121"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6639248.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGMQSSYPSIEQFNEFLTLNE"
     gene            2188..2970
                     /locus_tag="OO590_004122"
     CDS             2188..2970
                     /locus_tag="OO590_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR6639249.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPALSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(2972..3198)
                     /locus_tag="OO590_004123"
                     /pseudo
     CDS             complement(2972..3198)
                     /locus_tag="OO590_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT         1002 a          772 c          671 g          935 t
ORIGIN      
        1 tcataaggat gttggctata aacttgccaa taaagaagca ggcaaagctg tagactatga
       61 agtttgaagc atcacgttcg ttgatatcgc ccaactccag tgccagacgg atggtaaatg
      121 accatactgc gacctgcata cccacataaa ggaattgagc cacaatacca cgacgaaagc
      181 gcgaattttt agccagatag cgcagcgtat ccattgctga cgggcgttta tggtgacttg
      241 tctgtgccac tttacaggtt gggaagcggg ttaaaaggaa caacaccatg accacaacca
      301 gaatcataat catatactta tacggttcaa gggtgttctc taacatcagc accttaaagt
      361 tgtgaatttg ctcggcgttc attccggaca tctgcttctc aaggctttcc ccctcggaga
      421 aaaccagata tttgcccaat aaaataccag acgcagcacc aatcggataa aaggtctggc
      481 tgatattgag ccgcaatgtg gcataggctt ttggaccgat cattgaactg tatgtgttcg
      541 ctgcagtttc aaggaaactc aggccaatcg caatcgcaaa aatagctgca aggaacatag
      601 tgtaggttgc catatgcgag gcagggaaaa aaagtgtaca accaccaata tacagcgtca
      661 ggccaattaa aattgccacc ttataactgg tctttttaat cacaagggat gctggtattg
      721 caattaaaaa ataacctcca taaaatgcgc tctgcaccaa tgctgaagca aagttactta
      781 gcgaaaatac acttttgaat tgagtgatta atatgtcatt taatgcagct gcgcatcccc
      841 atagcgggaa taaacacgat aacaaaataa actggaacaa gggagtctta ttcagatacc
      901 catcaggcat ctgaatgatg tttttatcgt tcatagtgct acctttaact gtgcaggatg
      961 attattcgtt taaggttaaa aattcattaa attgttcaat actcggataa gatgattgca
     1021 tacctttccc tgtgacgctg aaagcggcaa agagagcggc ttttttcaaa gcggcttcaa
     1081 catcaccgct ttgaacataa taatgggaaa agcaaccaat aaatgcgtca ccagcgccac
     1141 tagtatcaac agcatttact ttgaatgcag gaacatggac ttcctgatcg cgggtcatcc
     1201 ataatgcgcc tttttcgctc atggtaacaa taatattgtt cagcccttta tcaactaacg
     1261 aacgtgcggc caaacgaata tgatcataag tatcaaccga cataccagtt aatatttcca
     1321 gttctgtttc attcgggata aagaaatcac ctttgcaggc ataagacata tctaactcac
     1381 gcaatgccgg agccggattt aataacactt caataccatt tttcttacca aactcaatcg
     1441 cgtggtaaac tgtttccagt tgaacttcca gttgtaaaac gatcaatttg cattttttca
     1501 gatcttctgc agctcgatcg atatcttccg gggaaagaaa tttattcgct cccttaatta
     1561 ttaatatact attgctcgag ttggcattaa caaagatcgg tgcaacacca ctgctggtac
     1621 aggggacctt ctcaacataa gtggtattaa ttccccatga ttcaagatta cgaatagtat
     1681 tatccgcaaa aatatcatca cctactttag tcagcatcag gacttttgaa ttcaatttag
     1741 ccgccgccac cgcttgatta gcacctttcc caccacatcc gattttgaag gcaggtgctt
     1801 ccagagtttc tccttcttta ggcatctgat tagtgtaagt aatgagatcc accatattgg
     1861 aaccaataac tgcaatgtcc atttcactac ctcttataaa ctttcgcata acaatggtat
     1921 ttaaataaca ttagcatgtt acttttgcat catttgtgac tgagatcgcg attagcacat
     1981 caacccgatg tttatttaat agacttccag tctcatcact caggccaaca ctatctaatc
     2041 ataagcaacc taacaagatt agtgcccaaa attcagcagc ctataccctt ttcatttcaa
     2101 aggggcggtc gtatagtatg gtaatgaaaa caatgtttac taacgccaaa atgttatttt
     2161 tataacattc ttacggagag agagttgatg gaaacgaagc aaaaagagcg tatccgacgt
     2221 ttgatggaac tgcttaagaa aaccgacaga atccatttga aagacgcagc gcgaatgctg
     2281 gaagtttctg taatgactat tcgtcgcgat ctccatcagg aagatgaacc tctgccactg
     2341 accctactgg gtggctatat tgtaatggtg aataaacccg cgctatccat gccagtaatc
     2401 catgacgttc caaaaaatca tcgtgatgac ttacctattg caattctggc tgccggaatg
     2461 gttaatgaaa atgatctgat cttctttgat aatggccagg agataccact cgttataagc
     2521 atgatcccgg atgcaatcac cttcaccggc atctgttact cacatcgcgt ctttgttgcg
     2581 ttgaatgaaa agcctaatgt aacagcaata ctttgtggtg gtacgtatcg tgccagaagt
     2641 gatgcttttt acgatgccag taactcttcg ccattagact ctctcaatcc gcgaaaaata
     2701 tttatttccg ccagcggtgt gcataatcac tttggcgtca gctggtttaa ccctgaagat
     2761 cttgccacta agcgtaaagc gatgaaccgt ggactacgga aaattttgct cgcccgccac
     2821 gcgttgttcg atgaagtggc ctctgccagc ctcgcaccga tctctgcatt tgacgttctg
     2881 attagcgatc gtccgttacc ggcagattat gttacgcact gccagaatgg ttctgtaaag
     2941 atcattacac ctgattcaga agacgaatga cttactgaaa aaacaccaca atcttgttaa
     3001 acatcgtcgg gttggactga ttacgttgca ctttcaccac atattccagc ttatctattt
     3061 ggcttatcac ctactccaga cgctggtcat ccttgaccag tagccagata tggcttttgt
     3121 cgcagtcctg aatcggcagg cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag
     3181 cccacaaagg tgagtcatta cacccggatg gtaaacaggc acttccttgt tgtagaagcc
     3241 gttatattgt ccataatgtc tgggttttta atgcatactg ggtaacaacc ggacgaatct
     3301 tcgaatatca ctggcgctgc cagccggttt gaattcatct cacaaccctg catagagcga
     3361 atctcttgcc tgcacgtcac
//
LOCUS       DAJTYF010000226         3271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTYF010000226 DAJTYF010000000
VERSION     DAJTYF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.229"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1058..1498
                     /locus_tag="OO590_004124"
     CDS             1058..1498
                     /locus_tag="OO590_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639250.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(1577..>3271)
                     /gene="treA"
                     /locus_tag="OO590_004125"
     CDS             complement(1577..>3271)
                     /gene="treA"
                     /locus_tag="OO590_004125"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6639251.1"
                     /translation="KSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILLG
                     PLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPKE
                     GEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWDS
                     YFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELLA
                     QHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPR
                     PESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVPV
                     DLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDLK
                     SHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAPN
                     GWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGGG
                     GEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
BASE COUNT          833 a          732 c          778 g          928 t
ORIGIN      
        1 tcctgaaaag caaaatcgat atatactttc atatatatcg ataaaaatca tgttcaatat
       61 caaagtttag ctattgaaaa catgaagatg tgacgacttc cgcaagcctg ttgaccctct
      121 ctatcattaa aaatgtattt atcaggtggc taatattatt taaatcattt aaggctaaat
      181 catttaaata tgaatgacat tattgttttt tatcgtccac ggttacgcga cgttgaagtt
      241 acttgtttta caatgagcga tttttgatat ctcgccgaga aaattatcag cattctctcg
      301 tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga atgctggggg ttatacaaaa
      361 cctatgttgt tgtcgtccgg attcaccgct tgctgatagt aagcagcgta accagaagtt
      421 accgcaacaa gactttgagg attttaagaa cgacgttatt aatgtcatta acaacaatga
      481 agatatttat tttcttagag agagtaattt actgaatgcg tttattcgaa cgtgcaaaaa
      541 aagagatgat aacagttacg gaaatatgat atttacgctt tattttgata acgatatcta
      601 tgggaacaga attatcaaag catcgctgaa cggcggagag ttgcagttat taagcacagg
      661 gtgtagcaat gataacattc tccattttat taatgcattt ctcattcggg tgaaatttcc
      721 gctacctgga cgcataaaac cggaactctc gccaaaagcc gggcactccg aaggaattga
      781 cgcgttgcgc tgctctgtaa cggcatttac cgcaagccat ttatttcgcc agcgacaatc
      841 cgcagagaac caaacgcatc aggaaacgac tgaacacaat aaatactgta ttgatcaaca
      901 tattatactg gcacctccag cacgcagtga ggatacactg tatcatcagc gaatcttatc
      961 actgcggagc tggttgaacg actttctgag cgaaataagt cggttcccgg taacaacaac
     1021 agaatccttt tttaattatt gtttcgttgt ttttggtgtg attcagaaaa taaatatttt
     1081 agacagtagc cagtcaggcg ttaaaaatgg ggcggatagt gaggcggttt tggctgcgct
     1141 tattgaacat ttagaattga tcaatccttc tggcagatta agccaaaata cccgttcggc
     1201 aatgcttcag ttacgcgaag aggaatggtc tgaattcttt ttttggttgc taaattctct
     1261 ggagtgtctg gattatgtga ttattaattt aactccagaa agcaaaaaaa cgttgatgag
     1321 tgaacaccgt aacaatattc aggtagcaat tgatgcttta tatagccaac gtcggcgtaa
     1381 atcgccgggt gatgaaagtg aaacacttac ccgtagaaat gacgctatct tcggcaacca
     1441 cgtatggcaa acatttgcgc aatacttccc gccaggactt gaaaaaccgt ctgtgtagaa
     1501 aaaaccatag atatggtgtt atctgccgac aacgccatcc ggagaagccg aatgacgttg
     1561 tcggagtaag cgctggttaa ggtgtgggtt gtgcctcttt ggttgagggt tgcgtcgttg
     1621 ctgacttaac ggtcggacgc gtcgccggaa cattgtcaca cggttgctct ttcgggcaga
     1681 tcaaatccag cattttcagc gtcacgccat tggtccagcc aaagccatcc tgtaatggat
     1741 attcgccacc gccgcccccc gttccggtgg tgctgacatc atatttttcc accagctttt
     1801 tctcccggtc ataggtgtgc tgaacattgg tcaggaagtg ccagctaatg tccatcgcca
     1861 cctctttttg cccgtagttt tgtaatcctt ctgtcgcgac ccactgtaac ggtgcccagc
     1921 catttggcgc atcccattgt tgcccacttt tcaccgacgt ggtgttcagg ccgccgggtt
     1981 gcagcagatg tgttttcgtc gccgtcgcca ttttgttggc gcgatctttc gctgccgcat
     2041 tgacgtacag cgggaacagg gcggccgcgg ttaactgatt gcgcacttta tgacttttca
     2101 ggtcgtaatc ggcataccag ccttgttgat cgttccacag gtatttttcg atcccttttt
     2161 gacgggcatt cgccagcgtt tcgtactggt ttgccatcgc gttatctcct gcagctttgc
     2221 tggcgcgggc gaggattttt tccattttaa acatcaggct gttcagatcg accggtacga
     2281 tgctggtggt gcgtaaggta tttaactgct gcgggttgtc catccagcgc gagctgaaat
     2341 cccagccaga cgcagcggca gagcgcaggt cgcggtaaat ttcagtggca ggtcgattcg
     2401 gattgctttt ggcggtggca atatcttcca cccatgactc tggtcgtggc gtatcgcgat
     2461 cgtcccagta gcggttgaga agggtaccat cctgaagttt tacaacgcgt ttttcctgtt
     2521 gtccggcttg caggttttca acaccgtcca tccagtaagc atattctttt tgcatttgcg
     2581 gcaggtattg cttcaacgcg gcatcgcctt catgctgcgc cagtaactct accatcaggg
     2641 caaagaaggg cggttgcgag cggcttaaat agtaactgcg gttgccgttg ggaatatgac
     2701 cgtaagtgtc tatttcatga gcaaaattgg ccaccatatc cgcgacttta tcccagtgac
     2761 cgctttcggc aagtcctaac atggtgaagt aactgtccca gtaatatacc tcgcgaaaac
     2821 gtccgcccgg cacgacataa ggttccggca gcggtaacag agaatcccat ttttcggtgt
     2881 tttcggtaga acgcgttaat accggccaaa gtccgtcaat atgttcgcgc agtgactgcc
     2941 cctctggcgg aacatatttc tcgccttctt tcggcagggt gaaattgacg ttaacgaaat
     3001 ggcgcagatc aaatccgctc tggttttgct gcatccgata atcagcaagg atcatcagcg
     3061 gatcgctgtt cggcacggca tcggcaaagg ttttttggtc cggaaaaagt ttggcgtttt
     3121 gcacatcatt aaacagcggc cctaataaaa tatcaggcgg ctgtggtgtt accggtgttt
     3181 cttctgcctg caccgatagc gcagcgaaac acaaaaagat acaggctgga attaacgcca
     3241 ttttttgcgg gcgagaaggt gcgggggatt t
//
LOCUS       DAJTYF010000227         3177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTYF010000227 DAJTYF010000000
VERSION     DAJTYF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.230"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            847..1041
                     /locus_tag="OO590_004126"
     CDS             847..1041
                     /locus_tag="OO590_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6639252.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESEIGT"
     gene            complement(2066..3175)
                     /gene="fepE"
                     /locus_tag="OO590_004127"
     CDS             complement(2066..3175)
                     /gene="fepE"
                     /locus_tag="OO590_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6639253.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          941 a          782 c          657 g          797 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagata tttaacataa tatcttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcccc ccccctaata taagatgatg tttttttatg
      421 aaaatagtca gccccccccc taaaaagcgg tgttggcgca gctacaacac cgcgccgaca
      481 ccgctttttt aaatatcaca aagagagcaa gaagaactaa tttgtcataa ctctctattt
      541 ataaagaaaa atcagcaaaa acttgttttt gcgtggggtg tgggcatttc aggcatgaga
      601 acagactcgc gtttttccgg agtatcagcg gaattattga ttaaggaaca ctcgacgcag
      661 agcgaaattt tatgcaacag cggctgcact gcacagggat tatttatcag ttgatatgct
      721 gtgccatgat acgtcctaaa gaaacaggac aggatgtgca attgtaatac cgtcgcccgc
      781 aacgtcttac aattaccacc tgctcagccc cccccgacac ccggaactga cggagaacaa
      841 cggaaggtga acaaacaaca acaaactgcg ctgaatatgg cgcgatgtat caaaagccag
      901 agcctgacac tgcttgaaaa actggatgct ctggatgccg acgaacaggc ggccatgtgc
      961 aaacgactgc acgaactcgc agaagaactc cagaacagca tccagattcg ctttgaggcc
     1021 gaaagtgaaa taggaacata acgaagctga cggaggcggt cacggctttc ccgtgggcgt
     1081 tttcgtcagc agacaagccc gtcagggcgc gtcagggggt tttagcaggt gtcggggcgc
     1141 agccatgacc cagtcacgta gcgatagcgg agtatatact ggcttaatca tgcggcggca
     1201 gagcaaatgg tgagagaaca gcgacatatg aggtgtgaaa tgctgcacag atacgtaagg
     1261 agaaaatgca gcttccggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1321 gttcggctgc ggcgagcggt gtctgctcac gcaaagacgg atatacggtt atccacagaa
     1381 tcaggggata acaccagaaa aaacatgtga gcaaaaaaga ggccccgaaa aaaggccgcg
     1441 ccggtggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa ccgacgctca
     1501 agtcagaggt ggcgaaaccc gacaggactt aaagatacca ggcgtttccc cctggtggct
     1561 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     1621 cgcgttcgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
     1681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1741 cttgagtcca acccggtaag acacgactta acgccattgg cagcggccac tggtaaacag
     1801 gatgtgcgag agatgtattg agagttcttg aagtggcagc cttactgagg ctacactaga
     1861 aggacagatt tggtgacatg tctcgcacaa gactgttacc acggttcaga agttccccaa
     1921 cttactgaac cttcgaaaaa ccacctcccc aggtggtttt ttcgttttct agagtagaag
     1981 actacggctg taagggaatg atctcaagaa gatcctttga actttttata aaagttgctt
     2041 ataaaatgat gcatcagcac aacttttact caatcacact catctttctg gcctccatgg
     2101 catgacgtaa aagtatatag ccacatgcaa gcatccctcc aatgacagaa gaaagaatta
     2161 caattatggc attacttggc ccatcctttc tgaccggtaa cgttgggctc aattgatact
     2221 gaaatggcat aaaatcaaca tcccccactt tcgccacaac caattgattc acaacatatt
     2281 gacgatttcg caactcccca ctcaattccg aaacatcctt gattgatttt ttgatgttca
     2341 atttagttgc tataccatca gaaccgagag ccacaggaaa atctgggtca tccttcataa
     2401 tctgaccatt gctgtataca ggttttttta ttccagccgc attagcaacc tccagtgaat
     2461 aattgaggcg tcgaatatct gcattcagct ggtttctaat ttttatctca tccagtgcaa
     2521 gaatttcttt ttcaacatta gttttaactt ctagtttatt tcttatatct tccatcaaat
     2581 cccttacaac aagggaagaa acataattaa tatacccaga caacacatcg tgagcttctt
     2641 tacttgttgg agcagtaaaa ctcagagtcc atgatacata caatgatgac ttgtcgtttt
     2701 tcttactggc attatcatca atcgacttca tatttttact caactcaaca attgcacgat
     2761 gcagttcgag agcgctaatg ttattctttt taataatctc cataacataa ggagatgatc
     2821 tcatatactc attcaacgaa ttaacggact gaaatttctt aataaacaga gcaaatgcgc
     2881 tatcacgatt gatatcaaaa tccactccca gaacatgaag cttggaaagc tccttattta
     2941 attcactcca ttgaacagac tcagcaggag taaccacagc agaactagtc catttttgtg
     3001 gcaaaacaaa agaaacaaga aagccaatac aagcaaaaaa tagaatcacc ccaacaattt
     3061 ttatttttgc tgcccataaa acgctaatca agccaaaaag gtcaatttct tcattaccat
     3121 tcggcaacat gttgtaatta gaaaaatgtt cgtcagagac ctttgagatt ttcattg
//
LOCUS       DAJTYF010000228         3142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTYF010000228 DAJTYF010000000
VERSION     DAJTYF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.231"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="OO590_004128"
     CDS             complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="OO590_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6639254.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQ"
     gene            complement(1002..1097)
                     /locus_tag="OO590_004129"
                     /pseudo
     CDS             complement(1002..1097)
                     /locus_tag="OO590_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="OO590_004130"
     CDS             complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="OO590_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6639255.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="OO590_004131"
     CDS             complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="OO590_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6639256.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2194..3142)
                     /locus_tag="OO590_004132"
                     /pseudo
     CDS             complement(2194..3142)
                     /locus_tag="OO590_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          781 a          762 c          762 g          837 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa
      121 cattcattcc tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa
      181 aaatactctc tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa
      241 ttacgggtcg ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg
      301 ttatagctga tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca
      361 ttgtgttgta cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta
      421 ttgctggcgg aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga
      481 ttatatgata cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga
      541 tttaaacggt ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag
      601 acagcctcat cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa
      661 aagtaatcag catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag
      721 ttattattta tcggtaacat gatgggcatc cagttaaagc cggtggacaa atggctttgt
      781 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      841 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      901 gtgaatggcc tgtttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc
      961 atatttgaac ggtaagcggc tcgtcagaac cgtattgata tttactgaga ggtcagatca
     1021 actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat atgctcaatg
     1081 acgtaacgca gccattttgt caacgccacg atttttgacc ctagcctgac atttatattc
     1141 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1201 cttcttcccc gataacgaag atcggcacac tggccatatc agtggtcatc atgcgccagc
     1261 tttcatcccc gacatgcacc accgggtaaa gctcacggga gactttatct gacagcaggc
     1321 gggcactggc cagggggatc accatccgtc gtccgggcgt gtcaataatg tcactctgta
     1381 catccacaaa cagacgataa cgactctctc ttttataggc gtaaacctta aactgcattt
     1441 caccagtccc tattctcatc ggcaaaagag ccgttcattt caataaaacg ggcgatctca
     1501 gccatcccct gctgattttc agcctgccag cgttccgcac gcagacggcg ggcctctttt
     1561 tgcatggccg tatttaccag gcctgagata ttcacgtttg cggatttaag taactgatag
     1621 ctgtcactgt ctatagtgac agtaatacgc tgcttcatga taagccctca aaaacatact
     1681 tatgatatgt atatgagtat atattaatgg tacaaaccat caatatccat aaaaagtaca
     1741 ccattaattc gctataagtt tttaatacaa ttttttctgc tgcgattttt ttatactata
     1801 attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc gtgatccgat
     1861 ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg ccgggcaccc
     1921 ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct cagaagtact
     1981 gagtcgggtg atcgcgtcag gccgcaaagt actgacacgg gtattggtga ctggtgatga
     2041 agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt cagtccgcgt
     2101 ggttgctggc ctggctgatt tcctgttgtc aggcgttcaa caaccgactg caggttatag
     2161 cccggttcac gaacacctga cggcaggtat cactcacccg gtgaaggtaa atccctcaag
     2221 aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca ggacgtccgt
     2281 cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc tcattatctg
     2341 tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc gtcccataac
     2401 aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc cgtcacccag
     2461 gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat ttaatggccc
     2521 gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat tcactatcgg
     2581 ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac ggatgagttt
     2641 ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg gctccctgct
     2701 ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg ctgatgtgat
     2761 gccggggtga ttatccgcca gagatttgta cacgctgtat ccgtcactca ccagcgtacc
     2821 gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg tctgcgagtc
     2881 aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca gccagccgga
     2941 gcaggattta ccgcctttcc gggtatcaag gagcctcagg gacgtttcat ctgcatgaat
     3001 aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca gtgcagctcc
     3061 ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca ttcgggagaa
     3121 aatgtgctgc tggcgataca gg
//
LOCUS       DAJTYF010000229         3119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTYF010000229 DAJTYF010000000
VERSION     DAJTYF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.232"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..82
                     /gene="ldrD"
                     /locus_tag="OO590_004133"
     CDS             <1..82
                     /gene="ldrD"
                     /locus_tag="OO590_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR6639257.1"
                     /translation="FWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(169..1848)
                     /gene="bcsG"
                     /locus_tag="OO590_004134"
     CDS             complement(169..1848)
                     /gene="bcsG"
                     /locus_tag="OO590_004134"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6639258.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1845..2036)
                     /gene="bcsF"
                     /locus_tag="OO590_004135"
     CDS             complement(1845..2036)
                     /gene="bcsF"
                     /locus_tag="OO590_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6639259.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2033..3119)
                     /gene="bcsE"
                     /locus_tag="OO590_004136"
                     /pseudo
     CDS             complement(2033..3119)
                     /gene="bcsE"
                     /locus_tag="OO590_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
BASE COUNT          775 a          802 c          824 g          718 t
ORIGIN      
        1 cttctggcat gatttagcgg ctccggtcat tgctggcatt cttgccagta tgatcgtgaa
       61 ctggctgaat aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt
      121 gcgcccggat caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta
      181 aggcacccag tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac
      241 tgcatttgag ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca
      301 gtcaacattg tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat
      361 agccaggaag ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat
      421 gccgaagaat ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag
      481 gccagatacc tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac
      541 catcacttta cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa
      601 tttctgcgcc cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc
      661 atgcagtgga agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac
      721 gtccagccag cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag
      781 caaaataacc ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg
      841 aacttctttc aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa
      901 gcccagtttc gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt
      961 agtgtgcgaa gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta
     1021 ggaggtggcg gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga
     1081 catcaacccg gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag
     1141 ttcaaatggc tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc
     1201 cgcgttatag aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg
     1261 tgcgggcata tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt
     1321 accacccgtc gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg
     1381 tcccgccagg gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg
     1441 aatccattgt gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg
     1501 ccagttaata aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg
     1561 cgaaccctgg ctcattatgc tttccgggcc aagcaaccag gtgtcatgcc agaacaaagc
     1621 aaagccgatc ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg
     1681 catcagcaga aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc
     1741 ccacaacagg ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta
     1801 ttgccagagg gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc
     1861 ctggcttttt ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag
     1921 aacaaacgta aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg
     1981 aaaaatatca gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg
     2041 agcgctccac agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac
     2101 catcgtgctc ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc
     2161 attgttctgg ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa
     2221 accagaccat acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg
     2281 cggtatccag atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa
     2341 tggtcatgat atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga
     2401 tacccggtac cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt
     2461 tattcatcat gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt
     2521 tgagcggctg ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact
     2581 tctgccctta cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa
     2641 taaccatatt tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg
     2701 tattttcccg cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc
     2761 tgcgggccag tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct
     2821 gagcggtacg ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg
     2881 atagcggcgg cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc
     2941 gtggttggat ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa
     3001 cgctaagctg ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca
     3061 aatgttggtc accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctca
//
LOCUS       DAJTYF010000230         3094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTYF010000230 DAJTYF010000000
VERSION     DAJTYF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.233"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..1091)
                     /gene="cusS"
                     /locus_tag="OO590_004137"
     CDS             complement(<1..1091)
                     /gene="cusS"
                     /locus_tag="OO590_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6639260.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVG"
     gene            complement(1081..1764)
                     /gene="cusR"
                     /locus_tag="OO590_004138"
     CDS             complement(1081..1764)
                     /gene="cusR"
                     /locus_tag="OO590_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6639261.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            1921..>3094
                     /gene="cusC"
                     /locus_tag="OO590_004139"
     CDS             1921..>3094
                     /gene="cusC"
                     /locus_tag="OO590_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6639262.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKMEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQI"
BASE COUNT          764 a          796 c          812 g          722 t
ORIGIN      
        1 ccaacaaacc gcaactccac gccgcgatct tccgctagcg cctcgaaaaa atcgaacact
       61 ttgccgactt catccgccag gttgagcatt ttcttttcgg ggattagctg gttgttatcg
      121 gcctgagcga gaaacagcat atcgctgacc attttcgcca ttcgcgtcag ctcttcgaga
      181 ttagagtaga gcacatcttc cagctccttc tggctgcgcg actggctgag ggcgatttcc
      241 gtttgcgtaa tgagattagt aattggtgtg cgaatttcgt gggcgatatc cgctgagaaa
      301 ttggactggc gagtaaagac atcctcaata cgctcgatca tatggttgaa cgacagtacc
      361 agctgttcca gctcaatcgg cacggtctgc gggtcaaggc gaacgtcgag atctttcgag
      421 gtgatattct ggatttgacg gctgacgctg cggatcggcg cgtgaccttt atgtaccgcc
      481 aacagcacga taaagacgat caggatgctg attaccgatg cggtcataat aagtttattc
      541 atcaaatcat ttatgtaatg aagatgaaaa tcgatcgaca gcgcgatgta gagcgtataa
      601 atcggtttgc cgtccaccaa cgggccaacc ggcaagttaa tcatccgcca gttgctgtgt
      661 tccatatgcc cgtgaccgtg gcctggcatc ataatcgtcg ggccggaaag gagatacacc
      721 tcgccacccc gcgcgtcttt atcgggtatg gcatcacgtg caaactcgcg gatatcaggc
      781 gcaccggggg agtgatacac cgttttgccg tggctatctg ccagggaaat caacacgttg
      841 gaataaccac tgacgatatc ttccagcgtc attaagcgtc gggcttgcgt ttcgtcaggg
      901 tgatttagta cccgttcaag ggtggcgcta atctctttta aatcattaat gtcctgctcg
      961 gcaaaatgca cttttactga gtggatcatg atccatgcaa aggcgaaaaa cgccgcgatg
     1021 gtggccaggc tgataaaaaa ggtcaggcgg gttgccagcg aaaacgggcg ctgaaatggc
     1081 ttactgacca tccggcacct caagcatgta acccacgccg cgcacggttt gaatcagctt
     1141 cggctcaaag tcgttgtcga ttttgccgcg tagccgcttc accgccacat caatggcgtt
     1201 agtgtcgctg tcgaaattca tgtcccatac ctgcgaggcg ataagcgagc ggggcagcac
     1261 ttcgccctga tggcggagaa aaaactccag cagagtaaat tctttactgg tcagagtgat
     1321 gcgcgtgccg ctgcgggtga ctttgcggct gacgagatcg accatcagat cggcaacctg
     1381 aaactgactt tcgataatca ccgccgcccc gcgccgcagg agagtgcgca cccgcgccag
     1441 cagttcagca aaagcgaacg gtttcaccag gtagtcatct gcccccaact ccagcccctt
     1501 gacgcgatgt tcaatggtgc caagcgcggt aagcaacaga atcggcatcc ctttattggc
     1561 ggagcgcaac atgcgcacga tatcccagcc gttcacgtct ggcagcataa tatcgaggat
     1621 tatcaggtca taatcaccgg tcatcgccag atggtagcca ttcagcccgt tgtcggccaa
     1681 atcgaccaca aaaccggctt cggttaaccc tttggtcaag tattctccgg ttttcttttc
     1741 atcttcgaca atcaacagtt tcatatttcc tccgcatgtt gcccgggcaa ttctagagta
     1801 gcgggatcag atggcaatcg cttattggca aaatgacaat tttgtcattt tcctgtcacc
     1861 ggaaaatcag agcctggcga gtaaagttgg cgacataaaa tcaccagaaa ttatgagcct
     1921 atgtctcctt gtaaacttct gccattttgt gtggcccttg cgctaactgg ttgttcactg
     1981 gcaccggatt atcagcgtcc ggtaatgccc gtaccgcagc agttctcact cagccagaac
     2041 ggcctggtta acgcagcgga taactaccag aacgcgggct ggcgcacctt ttttgttgat
     2101 aatcaggtga agacgctgat tagcgaggcg ctggtgaata accgggattt gcgcatggcg
     2161 acgctgaaag tgcaggaggc acgggcgcaa tatcgtctga ccgatgccga ccgctaccca
     2221 cagctcaatg gcgagggcag cggcagctgg agcggcaatc ttaaaggcga ttcagccacg
     2281 acgcgggagt tctcgactgg ccttaacgcc agctttgatc tcgatttttt cggtcgctta
     2341 aagaacatga gcgaagccga gcgccaaaat tatttagcca ctgaggaagc ccagcgcgcg
     2401 gtgcatattt tgctggtttc taatgtcgcg caaagctatt tcaatcagca gctggcgtat
     2461 gcgcaattgc aaatagccga agaaacgctg cgtaattatc agcagtcata tgcgtttgtc
     2521 gaaaaacaac tgttgaccgg tagcagcaat gttctggcgc tggaacaggc tcgcggggtg
     2581 atagaaagta cccgcagtga catcgctaaa cgtcaggggg aactggcgca ggcgaataat
     2641 gcattgcaac tgttattggg aagctacggc aagctaccgc aagcgcagac agtaaacagc
     2701 ggcagcctgc aaagcgttaa attaccggcg ggcttgtcgt cgcaaatctt attgcagcgc
     2761 ccggatatta tggaagctga acacgcgtta atggcggcta atgctaatat tggtgctgcg
     2821 cgtgcggcat ttttcccgtc tatcagcctg accagcggaa tatcaaccgc cagcagcgat
     2881 ctgtcgtcat tatttaacgc tagcagcggg atgtggaatt ttattcctaa aatggagatc
     2941 cccattttta atgccggacg caaccaggcc aatctggata tcgccgaaat tcgccagcag
     3001 cagtcggtgg tgaattatga acagaaaatc cagaacgctt ttaaagaggt ggcagatgcg
     3061 ctggcattac gtcaaagcct ggacgatcaa atca
//
LOCUS       DAJTYF010000231         3003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTYF010000231 DAJTYF010000000
VERSION     DAJTYF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.234"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(195..2036)
                     /locus_tag="OO590_004140"
     CDS             complement(195..2036)
                     /locus_tag="OO590_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6639263.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(2036..>3003)
                     /locus_tag="OO590_004141"
     CDS             complement(2036..>3003)
                     /locus_tag="OO590_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6639264.1"
                     /translation="ELARVGVDPNSVAQMYQQNPSGFGEFVDHLGMAALGPIDYFNVQ
                     DKMAGREIDRGRLAETIRSNQAGEALQARGQNLSYQSAMTGHNIAAQRLALDQQEFGF
                     KMQQAQEKAQQLISEAPKLSVNMEKGIETAVNNATASSNSANSMSALAQQFRAEKPTT
                     GLFGNAQNMFAKLTGSDTTLRDLRIRQNALVNSQVLKFLPPGPATDKDVEIVRQGAPT
                     DMDNPETVARWLDAMANLERRNAQFNEFKAEWMSANGNPGQSRNGGQILGLDVKKGES
                     LGSAVKRYMSMNTDAAPAQDSTPSGEPRNQVGSYTSKSGIQFTVE"
BASE COUNT          682 a          755 c          654 g          912 t
ORIGIN      
        1 gccatagtga agagttgcga aggcatcgat acgatcacat tggctgtaat gtctgtcatt
       61 taatttgctc cagatacaat gaatcgccgc agcgtggcta cggtgaattt tgggcataaa
      121 aaaaaccagc cgaagctgga tagttgcgtt ggttatctgt cagtagttat gtgcttattt
      181 aattatttat ctatctattg ttctgctgta atttggcttg aaagtatagg gcgtatcgca
      241 tttgcagcat tatttaacgc tctttcataa gctgccgttc ctgctttggt gtttgccaaa
      301 cgaagaagca tattccttgc ggctttggac tcatacaaac gcatcattgc accaaaacca
      361 gcctcaagcc ccattgatac accaagagtc gcagttgcgc caatcgtcct tatcctgttg
      421 gcttgcgatt gccccgtctg agttactaca tttgcggtgt ctgaccttgc tgtttgctgt
      481 agaacttcat gaagagcatc aagctctttc atgtgctttc cagaaaaaat agtgttgtaa
      541 atttcaccgc ctgactgaga tttcagctta ttaacttcag tgatgaactt ggctggagag
      601 tcaccggcct tttccgctat tttgctgacg taagctgcac gcatagcatc tttcccttta
      661 tcatccagtg cgctccagat tcgtttcacg tcagatggtt ttctgcttaa tacaacggta
      721 tttataagtt caggactggc ttcactgctt gccttgttga gcttgttagc aatgttttta
      781 ttaagcacct tattataaac gtttgcataa tcggaatttg ctttaaggta ttttgctgcg
      841 tctgatgcac cgaggttttt agcaactgcg ttacgaaggt cttttgacat tgcattctct
      901 accatattgg tagctgcttt tgcctggttg gggaagacca tagcatctcc ctgaacatta
      961 gatctaaatg ctgttctgtg ctgacgcaag agatcaaacg taacatccaa atcagttgca
     1021 gggtttgcta attcttcacg taggttacgc aaggatgtaa gcaggctttg attggcagac
     1081 gtcccaagcc gttcctgtct tgcgatcgct gtattcagag cattcatggt atttgtggta
     1141 tcaactgcgg cattacccat tttattggtg acgtcattga taacagcgcc agcggcatcc
     1201 ttccgccccc ttaacgtggt ggtaagagat ttcaccacat catcagggtt gtactcacca
     1261 aaacggtcaa aataattact taccagctta ctacgcgttg catattgctc cgctcgcttt
     1321 gagcctgtcc cgagcaaagc cccctcggca tcctgagtta ggccgcgagt gaaagcattt
     1381 ttcggcggga taacatcaga tgtcattggt gtcacgccca tcgattctga tgtggcaatt
     1441 ttcttcgcca cttctggcgc aatatcacct tttatagccg ttattccacg ccctattccc
     1501 tttgctgctg cggaaagaac accctgagcg gcaaggttaa ctccggcatt tttagctgca
     1561 ttttgtgcga aatcgccttt ctgatttgcg gcctctgcca gtgatccaat agccatgctt
     1621 cctgccgttc caactcctgg aactaaatac ccaccaattg tttcaccggc ttgcgcgtag
     1681 gggtctgtcg gtctgtctac tggacgataa acatcatcca aaaccttggg tccaccaagc
     1741 ccctgactga ttgcattaat cagacttgcg ccgccctgca atacgtcaaa tggtatgttt
     1801 accagaccac gaccagcctg ttctgcaatt tgccctgcac tttgaccacc agtgagccaa
     1861 tcgccagctt gttgcatcaa tgatggttct tcccgtgttg gtgcattatt ggcctgatta
     1921 actgtttgtt gctgaacagc ctgaccagca aaatactcat caatggcggt gccaatatct
     1981 tcggtgctcg taccatcagg aaaggtaaat gtcttaccgt ttgcagttac tttcatcatt
     2041 ccaccgtaaa ttgaatgcct gattttgagg tatatgatcc aacctgattc cgtggttctc
     2101 ctgaaggtgt cgaatcttgt gctggcgctg cgtcagtatt cattgacata taccgcttaa
     2161 cggcactccc caatgattca ccttttttaa catccaaccc caatatctga ccgccattac
     2221 gcgattgtcc agggttgcca ttcgcgctca tccactcggc tttaaactca ttaaactgcg
     2281 cgtttcgtcg ctcaaggttt gccattgcat caagccatct tgcgaccgtc tcagggttat
     2341 ccatgtcagt tggcgcaccc tgtcgaacga tctcaacgtc tttatccgtt gctgggccgg
     2401 gaggtaggaa tttaagaacc tgactgttaa caagggcatt ttggcgaatg cgcaaatcac
     2461 gcaatgttgt atcgcttccg gtaagttttg cgaacatgtt ctgtgcgtta ccgaacaaac
     2521 ctgtcgttgg tttttctgct ctgaactgtt gagcaagcgc actcatagaa ttggctgagt
     2581 ttgatgatgc tgtagcattg tttacagccg tctcgatgcc tttttccatg tttactgaca
     2641 gcttaggtgc ttcgctaatc aactgctgag ccttttcctg cgcttgctgc atcttaaacc
     2701 cgaactcttg ctgatccaga gccaagcgtt gtgctgcgat attgtgccca gtcattgctg
     2761 actgatagga aaggttttgc cctctcgcct gaagtgcttc tccagcctga ttgctgcgga
     2821 ttgtctctgc cagcctgcct cggtcaattt cacgaccagc catcttgtcc tgaacattga
     2881 agtaatcaat cggaccaaga gcagccattc caaggtgatc aacaaactca ccaaatcctg
     2941 aaggattctg ctgatacatc tgagcaacgc tgttagggtc aacaccgacg cgcgccagct
     3001 cat
//
LOCUS       DAJTYF010000232         2894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTYF010000232 DAJTYF010000000
VERSION     DAJTYF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.237"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..>2894
                     /locus_tag="OO590_004142"
     rRNA            1..>2894
                     /locus_tag="OO590_004142"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          763 a          634 c          909 g          588 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc acatactgtg
      361 agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc
      421 aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag
      481 aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc agtgggagca
      541 cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat attctgtagc
      601 aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg ttaagttgca
      661 gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg ggtaacacta
      721 actggaggac cgaaccgact aatgttgaaa aattagcgga tgacttgtgg ctgggggtga
      781 aaggccaatc aaaccgggag atagctggtt ctccccgaaa gctatttagg tagcgcctcg
      841 tgaattcatc tccgggggta gagcactgtt tcggcaaggg ggtcatcccg acttaccaac
      901 ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga gacacacggc gggtgctaac
      961 gtccgtcgtg aagagggaaa caacccagac cgccagctaa ggtcccaaag tcatggttaa
     1021 gtgggaaacg atgtgggaag gcccagacag ccaggatgtt ggcttagaag cagccatcat
     1081 ttaaagaaag cgtaatagct cactggtcga gtcggcctgc gcggaagatg taacggggct
     1141 aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt gttgggtagg ggagcgttct
     1201 gtaagcctgt gaaggtgtac tgtgaggtat gctggaggta tcagaagtgc gaatgctgac
     1261 ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg ttcctgtcca
     1321 acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg tagtcgatgg
     1381 gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga gaaggctatg
     1441 ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag gcaaatccgg
     1501 aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc tgaagcaaca aatgccctgc
     1561 ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac cgacacaggt
     1621 ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact aggcaaaatg
     1681 gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg aagcgacttg ctcgtggagc
     1741 tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca gcactgtgca
     1801 aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt taattgatgg
     1861 ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt aactataacg
     1921 gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat ggcgtaatga
     1981 tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa gatgcagtgt
     2041 acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac tgaacattga
     2101 gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct gcatggagcc
     2161 gaccttgaaa taccaccctt taatgtttga tgttctaacg tggacccgta atccgggttg
     2221 cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag taacggagga
     2281 gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg cataagccag
     2341 cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg atccggtggt
     2401 tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa caggctgata
     2461 ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct catcacatcc
     2521 tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt acgcgagctg
     2581 ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg agaactgagg
     2641 ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc gggttgtcat
     2701 gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag catctaagca
     2761 cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag gaacgttgaa
     2821 gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct aaccggtact
     2881 aatgaaccgt gagg
//
LOCUS       DAJTYF010000233         2864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTYF010000233 DAJTYF010000000
VERSION     DAJTYF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.238"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..451)
                     /gene="intS"
                     /locus_tag="OO590_004143"
     CDS             complement(<1..451)
                     /gene="intS"
                     /locus_tag="OO590_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6639265.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFED"
     gene            complement(613..687)
                     /locus_tag="OO590_004144"
     tRNA            complement(613..687)
                     /locus_tag="OO590_004144"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(652..654),aa:Arg,seq:cct)
     gene            complement(763..1695)
                     /locus_tag="OO590_004145"
     CDS             complement(763..1695)
                     /locus_tag="OO590_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6639266.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMVIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1989..2744
                     /gene="mlaA"
                     /locus_tag="OO590_004146"
     CDS             1989..2744
                     /gene="mlaA"
                     /locus_tag="OO590_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6639267.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPVRNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          694 a          693 c          688 g          789 t
ORIGIN      
        1 gatcttcaaa tctgcggatt acttccagca gttgcatcgg ctcaatatcc tgaatctcaa
       61 ggccgccgat gatgggtaaa atgtcgtcat caaacatttt tgcaagttca gtcgcatacc
      121 ctactgacca gacttgcttc ttgtgttcgt accattcctt gtaaatcgca ctaaatgaat
      181 tgttgttaga cgaagccttt ttcgccttta ccggatcgat gccagccgag atgtctttcc
      241 tcgcagtcca tgctttatcc cttgcctcct gcaaagtcat aagcggatat tttccgacgg
      301 tcaggatttt ctccttaccg tcaatcttgt agcgaagctg ccataccttt ttcccggata
      361 cagggacata aaggtacagg ccattaccat cgagaaggcg gtatggtttt tctttcggct
      421 ttgctgcttc aatctgctta acggtgagca tgggtaaaaa tccggtgggt aaaattattt
      481 tatccacttt ttacccgtca tggagtgcgg ctgtcaacga tctgacgcga accatgacga
      541 actgtgaata tacggaaggc ttgatattca ggggattttg cggactggta cggatgggag
      601 cgaactgata aatggtgtcc cctgcaggaa tcgaacctgc aattagccct taggaggggc
      661 tcgttatatc catttaacta agaggacaat gcggcatgag tatacccgct aatggactgc
      721 ggggtaagta cgctgccgct cgattgctta aaccctcgcc atttatgctg ggtttttatc
      781 atttttctta atgttttccg cacgttctgc tttttggcgt gcttctgctt tacgcttgtt
      841 gctcatgtcg ttacgaatct gtgcatgact cattaacgcg aagataaagg tgccgccgca
      901 gatgttcccc gctaaagtag gtagtgcgaa gggccagatg aaatcgctcc agtgcagcgt
      961 accgttaaac accagataga ggatttcaac agaaccgacc acgatatggg tggtgtcacc
     1021 cagggcaata agccaggtca tcaatataat caccacaatc tttgccgcac ctgctgcagg
     1081 aaacatccat accatagtgg cgatcagcca gccggaaatg atcgcgttgg caaacatctc
     1141 gctgggggtg ttcttcatca catccatgcc gattttgaca aatgcatcgc gagtttcttc
     1201 attgaagata ggcatatatt caaatgccca cgcagcaata ccagtcccga gaatattacc
     1261 cagcagcacg actccccata accgcataag taagccaacg ttgctcattg tcggtttttg
     1321 catgacgggt agtaccgcag tcacggtgtt ttcggtaaat aattgctggc gggccattat
     1381 gacgataata aaaccaaagg tataaccgag attctccagc aagaagctgc ccggcacacc
     1441 ttccagttcg acatgaaata tcccttttgc cagtaacgaa gcgcccatcg acagacccgc
     1501 cgcaatggct gaccacagta gcgccattgc gtcgcgttct agctcttttt caccatcctg
     1561 gcggatatgc tcatgaatta ccatcgctcg ggaggggagt cggtcttcat ctatttctat
     1621 ttttttgccg cgctcttttt cttcgctctc aacttcaatt tcgtcgctgt gttgatcaat
     1681 tttgtcgttg tccatggtct cttctcttga attagcacgt atagctaaag cgtagcggct
     1741 tttttgctcg caactggcgg gagttactct gaaaatgtag aaaaggttgc gtttgccttt
     1801 ttctgtttct atagaatcaa gtagcctaca gggcggcgat taccaggcta tgatcaaatc
     1861 agcaaatcag ggcgtctgga catcagttga cgtgctgtta caatcgccca cacctaaaca
     1921 ggcggatacg gtatcgttcc gtcatggatg gcaaactgca taagccataa aaaaacaggg
     1981 agacatttat gaagcttcgc ctgtcggcgc ttgctctggg aactacgctt ctggtggggt
     2041 gtgcgagttc cggtacagat cagcaagggc gttctgaccc gttagaaggg ttcaaccgca
     2101 ccatgtacaa cttcaacttc aacgtattag acccgtatat tgttcgaccg gtcgctgtcg
     2161 cctggcgtga ttatgttccg caaccggtgc gtaatggttt gagcaacttt actggcaacc
     2221 ttgaagaacc tgcggtgatg gttaactact tcttgcaggg cgacccttat caggggatgg
     2281 tccactttac ccgctttttc ctgaacacca ttttggggat gggcggcttt attgatgttg
     2341 cagggatggc gaacccgaaa ctgcaacgga ctgaacctca ccgcttcggt agtacgcttg
     2401 gtcattatgg cgtgggttat gggccttacg ttcagttacc gttctacggt agcttcacgc
     2461 tgcgtgatga cggtggtgat atggcggatg gtctttaccc ggttctttcc tggctgacct
     2521 ggccgatgtc tgtgggtaaa tggacgcttg aagggatcga aacccgcgct cagctgctgg
     2581 attccgatgg tctgctgcgt cagtcgtccg atccttatat tatggtgcgc gaagcgtact
     2641 tccagcgtca tgatttcatc gctaatggcg gcgaactcaa accgcaggaa aatccgaacg
     2701 cgcaagcgat tcaggatgat ttaaaagata tcgattctga ataagaaaca aataaaaaag
     2761 gtgagtcgca aaactcacct tttttgttgt tatccctcaa ttaccacaag taaaaggaga
     2821 taaaccgaca atgacccgga cgtaatgatg taaaaatact gatg
//
LOCUS       DAJTYF010000234         2798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTYF010000234 DAJTYF010000000
VERSION     DAJTYF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.239"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..420
                     /locus_tag="OO590_004147"
                     /pseudo
     CDS             1..420
                     /locus_tag="OO590_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1004..1720
                     /locus_tag="OO590_004148"
     CDS             1004..1720
                     /locus_tag="OO590_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639268.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSYKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2272..2676
                     /locus_tag="OO590_004149"
     CDS             2272..2676
                     /locus_tag="OO590_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639269.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
BASE COUNT          809 a          535 c          616 g          838 t
ORIGIN      
        1 catgatcggg gaagacccag atatgaacag gcacattcag agttacttta aagagcttac
       61 tgaacgtctg aggtgttaga gccatgactg ccgcagcatt gctggcggaa atgcctgaat
      121 cgagcagcct ctcgagacgt gaaatcagcg cacttgtcgg tgttgctcag gtaaaccggg
      181 actcgggaac tctgcgcggt cgacgaacca ttttcggtga ttgtgctggg gaagaacagc
      241 tctgtacatg gcggcgcttg aggcccccgg tttaatctgg tgataaaggc gttttacatg
      301 cgcctacttg cagccggaaa cgccaaaaaa gtggcactgg ttgcctgtat gcgtaagttg
      361 ctgaccatta tgaacgcaat gctcagaaaa aacgaagaat ggaatgaatc gtatttatga
      421 aaactggatt gtgggccata aatattccaa aaagatagcc cgctccgttt attaccggaa
      481 cgggcgagag catgacattg tctgttatta taatgagact gctgattcta cttattttta
      541 atttcaagaa aagcgttttc tacaaagaat tatgagattt aatgttttgt ttagacaata
      601 ttatcattct gttcaataat gaaagtcttc atcaacaaca agtaaaaaca acgtccctct
      661 catcgaaccg gacttgcaga tttcccgcat ccggctctct tgcaacacat cagaccttca
      721 cgttcgggcg gttatgggga aggtccagca agctgtatga gccctaactg tttgaatacc
      781 agtagattac cagaaacttc tatcgattga agacattacc aactagcgtt actaaatgtt
      841 gaccaagttt tgttcacttg tctggttttg atagtagcat tctgcagagt attttgatat
      901 gataaatatg cattaacaat gatattattg ttaatttcct agcataaaaa ttaaaagatc
      961 aacttttatc tcgaatattt tttacattat cagttggttt cttatgaatg acaattcatt
     1021 gcttagaaat tcttcattat tcatcgccta tatgggatgt gtagggtggg tcagtgctta
     1081 ttcttatggc tggggaacct ctttttacta tggatttcca tggtgggttg ttggtgcagg
     1141 tttggatgat gttgccagaa gtttactcta tgctatcata gtaatgggta tactttttac
     1201 cggttggggg attggtgttt tattttttct tcttataaag aaaagaagta aaatacaaga
     1261 cttgagtttt tttagactat tttttgctat taccctactg tttttccctg taatattcga
     1321 acttttaatt ctcaaacaat atttcatact tccattatca ctttcatgca ttattagttc
     1381 attggttatt tcaataataa ttcgtatata tggacggatc ttttctgtat catgtttttc
     1441 agatatccct tttgttagaa aacaccgcat taagttaatt atggctggtt ttttggttta
     1501 tttttggctt ttttcctttc ttgttggctg gtataaacct caattaaaaa aagaatatca
     1561 gatgctttgt tataataata gttggtatta tgttcttgct cgttatgatt cacggctcgt
     1621 tttgtctagt tcatataaag atgacagtaa cagattctta atatttaata cagaacagtc
     1681 gggattttat gaaatcaatg atgtttatgt cagaaagtaa taatcctctc ccacaaagtg
     1741 aattaaatga atatataatg cgttcgaagt atatgtattg agctgtcata ctgagaaaca
     1801 aaccttaggg cagcacaatt aaagccagaa acaactatgc aagaaagtta ctaaataagt
     1861 gctcggaaca gtaaaacact caggggggac tcagtccgga ttatgtaatc agataaattg
     1921 ccaaacatca caaatcacca gccggactga gtgatgccga gcattgcacc aataccccgt
     1981 gatgaacgat gcctgatgca gaaagctccc tataaaacaa agaacaaaaa ctacgaccgg
     2041 cgattcaccg tcatactgat gctgcatcgt ggtgacactg tctgtcactt tcatgttgac
     2101 tgaacaactc tgctgcgaag aaggtgtacc gccggcggga tttgattgtc cggcgtctag
     2161 cttccacttc cctgatggaa ctgaaagttg ttatggccgg tcagcttgcc ctgtccgcag
     2221 gacatgaggt gctgtgacgg acagcaccag taacggaaag tgacaatgaa aatgaatact
     2281 gcgctgatag ttgcactaat gtgtatgtgg tatgcggtac ccgctgctgc aaaagagact
     2341 ttactggcta tgccacgtaa ttcaacagaa cattgctatg cggagattaa tgtgcacgga
     2401 ccatatgggg tgtatttccg agtggtcccg catccccccg ggggaaagag ctgggtcgaa
     2461 tgtaacagtg actactatta ctctgataaa ccaccgggag tacagatact tggaacccgt
     2521 gcagggtgtc gggtatacgg aatctgcggg accacctcga cactgcatgt ggctggaaga
     2581 ggggttgtgt gtattaaaaa tatatgcagt ccacgtggga tgataattca caggatacgg
     2641 aaacgtcctg tagtggctgt aagcgatgag atgtgatctt acccagcaat agtggacacg
     2701 cggctaagtg agtaaactct cagtcagagg tgactcacat gacaaaaaca gtatcaacca
     2761 gcaaaaaaac acgtaaacag cattcgcctg aattttgc
//
LOCUS       DAJTYF010000235         2545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTYF010000235 DAJTYF010000000
VERSION     DAJTYF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.240"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..2049
                     /locus_tag="OO590_004150"
     CDS             <1..2049
                     /locus_tag="OO590_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6639270.1"
                     /translation="GKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDT
                     CELLDILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVI
                     DVEFSHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNS
                     KADEDVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASR
                     VYPGSSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEG
                     ATDLWEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQS
                     IQSLQSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDY
                     FRSGKRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRR
                     EALELMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARL
                     EDEGFTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTS
                     DWGWKEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCT
                     FREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML
                     "
     gene            2046..>2545
                     /locus_tag="OO590_004151"
     CDS             2046..>2545
                     /locus_tag="OO590_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR6639271.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGR"
BASE COUNT          688 a          615 c          718 g          524 t
ORIGIN      
        1 ggaaaaactg agccggtcct ccatctggaa catattcagc ccgtccgcaa tttattaagc
       61 acactgcaac aaaaactcga caccccggca ggacagcaag tcgcacagac cctgcagcaa
      121 accggggata cctgcgaact gctggatatt ctggcaaatg atggctggct caaaaatgaa
      181 taccacgggg aagacgaaat ctttaccgga ctggcatcgt taaacgatct ggttcgcctt
      241 gctgcggcaa tggggagtga gtttcctttt gatgaatacg cagaagtgca gaaactgccg
      301 gtgatcgacg tggaattcag ccatctggtg gggatggatg catgccaggg aacactcaca
      361 ctgctggata cccccggccc taatgaggcc ggacaaccgc agatggaagt gatgatgcgg
      421 gatcaactgc agaaagcctc tgcggtgctg gcggtgatgg attacaccca gatgaactca
      481 aaagcggatg aagacgtccg taaagagctt aatgccattg ctgacgtatc agccggccgc
      541 ctgtttgtgc tggtcaataa atttgatgag aaagaccgca atggcgatgg ggcagatgcc
      601 gtacgccaga aagttccggc aatgctgaac agcgatgtgc tgcccgcctc ccgcgtttat
      661 ccaggctcat cacgtcaggc gtacctggct aaccgtgcgc ttcatgagtt acggaaaaac
      721 agaacccttc ctgttgatga agcctgggtc gatgattttg tcagggaagc cttcggtcgc
      781 atgaaaaaag aatacgtctg taaagacagt gaaatggcaa ccgaaggggc gacagacctg
      841 tgggaaggct cacttatcga tcaactgata acggaagtca tacagagctc ccattccaga
      901 gcggcggcac tggcggttga ttctgccgcc gcaaaactga tgcagaatgc ggaaaatgtc
      961 agcgaatatc tgtcattacg ccatcagggg ctacagcaaa gcattcagtc actgcagtcg
     1021 catatcacca gcctgcttgc ggatatccgg gaaatcgcgg actgtcagga gcaggtgacc
     1081 gctgatgtca gaatggccat ggaggagatc gatgccagaa cccgggaatt actgacgggg
     1141 gtctgcacct cactggaaga ggagctgaat gactatttca gaagcggtaa acgcaaagaa
     1201 cagcaaatgc tggaggaaga aaattcagcg cagccccggg agagaaacgc attcgctttc
     1261 tttcacgata ttttcggaac cggaaatcag catgacagga tgcgggactt tgatccggac
     1321 agccccgaaa taaaattcag tgatcgcagg gaagcccttg aactgatgac gcaaatcgaa
     1381 tcgaccgtga ccagcctgca ccgtgaggct gaagcacagt tccgtcctga actggaaaaa
     1441 atcgtcaggg gtattgaaac aggttttcgt ggtacggccc tgtacgccac agaaaatatt
     1501 gccggacgca tcaatgcccg tctggaggat gaaggtttca ctgtaaaaat cagttttccg
     1561 gcagtcagcc agttacagac ccggctcgca gtaaaaacaa atctgagcgc gcttatggag
     1621 gaaagaacgg agacagtaac ccgtcgctgt cgacaggatg gtgtctgggg tacgctttgt
     1681 cgatgggcaa acaccagtga ctggggctgg aaagagtaca gtgtggatgt gagccgcagc
     1741 gtgatcaata tcaacaaggt cagagaggaa gtgatgtcac tgacccgggc atatttcggg
     1801 gagctgcagg catccattga gcaggatatt aaccagcccg tccgccagga aatcgatgcc
     1861 tttttctgta cattcaggga gaaagttgaa cagctgcgta acacgctgat tcagagctct
     1921 gaagatcata aacgcgatca gcaggcgcag gaacggctga ccgggcgtct tcaggcgtta
     1981 aatgaaaggg ttcctgagct cattactgac agtaaggcgt taagggaaga actggagaca
     2041 atgctgtgac ttcaccattt attcaacaaa ttgctgataa ccgggtatgt caggtactca
     2101 cctgtcttcc tgaaaaattt gtggttgatt tcgcaaacgg tattgatgtt gcacaggagc
     2161 atatccgtac ggccggagag cgcacgttct tccggcggtt aaaagagggg ctgagcggcg
     2221 aaggtactgc acggcagaat gccatcaatg cttcgcttgc acagggcgtt gaggcgtccc
     2281 tgcgctggct gacggagctg acaacgtcac ttgccaccac aaactacgcg attacccggg
     2341 taaacgacag ggtcagttca ctggtcagtg atactgccag gctggcgcat tattccgcag
     2401 atacgcggga gcagttactc accctggccg atcaggttca ccagaaactg aatcatcttg
     2461 aagaaaaact ccaccgtgtt gacctggttc agcgggcgca gttacatctt gagcagatat
     2521 tctcctggtg gagcgccggg cgata
//
LOCUS       DAJTYF010000236         2451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTYF010000236 DAJTYF010000000
VERSION     DAJTYF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.241"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..1632
                     /gene="kup"
                     /locus_tag="OO590_004152"
     CDS             <1..1632
                     /gene="kup"
                     /locus_tag="OO590_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6639272.1"
                     /translation="EGGILTLMSLAGRNTSARTTSMLVIMGLIGGSFFYGEVVITPAI
                     SVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKHGTAMVGKLFAPIMLTWFLILA
                     GLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIALGAVVLSITGVEALYADMGHFG
                     KFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNPFFLLAPDWALIPLLIIAALAT
                     VIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMESGQIYIPFVNWMLYVAVVIVIV
                     SFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHWNKYFVALILIAFLCVDIPLFT
                     ANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRRMHEHGNSLEAMIASLEKSPPV
                     RVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILLTLRTEDAPYVHNVRRVQIEQL
                     SPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMMETSFFMSHESLILGKRPWYLRL
                     RGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            1799..2218
                     /gene="rbsD"
                     /locus_tag="OO590_004153"
     CDS             1799..2218
                     /gene="rbsD"
                     /locus_tag="OO590_004153"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6639273.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2226..>2451
                     /locus_tag="OO590_004154"
     CDS             2226..>2451
                     /locus_tag="OO590_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6639274.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKS"
BASE COUNT          548 a          593 c          651 g          659 t
ORIGIN      
        1 gaggggggga tcctgacgtt gatgtcgctt gccgggcgta atacgtcggc gcgaaccaca
       61 tcaatgctgg tgattatggg gctaatcggc ggcagctttt tctatggtga agtcgtcata
      121 acacccgcta tttcggtgat gtcagccatt gaaggtctgg aaatcgtcgc cccgcagctg
      181 gatacctgga tagttcccct ctcaattatc gttctcactt tattatttat gattcaaaag
      241 catggcacgg ctatggtcgg taagctgttt gcgccgatca tgctgacctg gtttttgatt
      301 ctggcaggac tggggttacg tagcattatt gctaacccgg aagtgctgca tgcgctgaat
      361 ccgatgtggg cggtgcattt cttccttgaa tacaaaacgg tttcttttat tgcattaggg
      421 gcagtggtgc tgtcgattac gggggtcgag gcgctgtatg ctgatatggg gcactttggt
      481 aagttcccta ttcgcctggc gtggttcacc gtcgtattac cttccttgac ccttaattac
      541 ttcggtcagg gagcgttgtt gttaaagaac ccggaagcga ttaagaaccc gttcttcctg
      601 ttggcaccgg actgggcgct gatcccactg ctgatcatcg ccgcactggc gacggtaatt
      661 gcctcgcagg cggttatctc tggcgtcttc tcattgacgc gtcaggcggt acgtctggga
      721 tatttgtcgc cgatgcgcat tattcacacc tccgaaatgg agtcagggca aatctatatt
      781 ccgtttgtga actggatgct ctatgtcgcg gtcgtgattg tgattgtcag ctttgagcac
      841 tccagcaacc tggcggcggc gtacgggatt gcggtgaccg gaaccatggt actgacgtct
      901 attctctcga ctaccgtggc acgtcagaac tggcactgga ataagtattt tgttgcgctg
      961 atcctgattg ctttcctttg tgtcgatatt ccattgttca ccgctaacct cgataaactg
     1021 ctctccggcg gctggttgcc attgagcctc ggtactgtga tgtttatcgt gatgaccacc
     1081 tggaagagcg agcgtttccg cttgctgcgg cggatgcatg aacatggtaa ctctctggaa
     1141 gcgatgattg cttcgctgga gaaatcaccg cctgttcgcg tgcccgggac cgcggtgtat
     1201 atgtcgcgtg caatcaacgt cattcccttt gcgctgatgc ataaccttaa acataacaag
     1261 gtattgcatg agcgggtgat tttgttaact ctgcgcaccg aagacgcgcc atatgtccat
     1321 aacgtccgtc gggtacagat tgaacaactg tcgcccactt tctggcgcgt ggtggcaagt
     1381 tatggttggc gagaaacgcc aaacgtggaa gaagttttcc accgctgcgg tctggaagga
     1441 ttaagttgcc ggatgatgga aacctccttc tttatgtcgc atgagtcgtt gatcctcggc
     1501 aaacgcccgt ggtatttgcg tctgcgcggc aagctgtact tgctgctgca acgtaatgcg
     1561 ctgcgtgcac cagatcaatt tgaaatcccg ccaaacaggg ttatcgaact gggtactcag
     1621 gtcgaaatct aacgccagac gcctcctttc ttcataaggg ggcgtttttg ttttcatggt
     1681 taatcaccat gtaaaacgtt tcgaggttga tcacatttcc gtaacgtcac gatggttttc
     1741 ccaactcagt caggattaag ctgcggatca gcgaaacgtt tcgctgatgg agaaaaaaat
     1801 gaaaaaaggc accgttctta attctgatat ttcatcggtg atctcccgtc tgggacatac
     1861 cgatacgctg gtggtgtgtg atgctggttt acccatcccc aaaagtacaa cgcgtatcga
     1921 tatggcatta acccaaggtg taccttcttt tatgcaggtg ctgggcgtcg tcacaaatga
     1981 aatgcaggtc gaggctgcca ttatcgcgga agagatcaaa caacataatc cgcaactcca
     2041 cgaaacgttg ctcactcacc ttgagcagct gcaaaaacac cagggaaata ccattgaaat
     2101 tcgttacacc acgcatgaac aattcaaaca acaaaccgca gaaagtcagg cggtaattcg
     2161 cagcggagaa tgttctccgt atgcgaatat cattctctgt gctggcgtga cgttctgagg
     2221 ccgtcatgga agcattactt cagcttaaag gcatcgataa agccttcccg ggtgtaaaag
     2281 ccctctcggg cgcagcgtta aatgtctatc cgggccgtgt gatggcgctg gtgggcgaaa
     2341 acggcgcggg taaatccacc atgatgaaag tgcttactgg tatctatact cgcgatgccg
     2401 gcacgctttt atggctgggg aaagaaacga catttaccgg gccgaaatct t
//
LOCUS       DAJTYF010000237         2439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTYF010000237 DAJTYF010000000
VERSION     DAJTYF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.242"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..564
                     /locus_tag="OO590_004155"
     CDS             <1..564
                     /locus_tag="OO590_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6639275.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            522..1199
                     /gene="bglJ"
                     /locus_tag="OO590_004156"
     CDS             522..1199
                     /gene="bglJ"
                     /locus_tag="OO590_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6639276.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQEFFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1237..2025)
                     /gene="fhuF"
                     /locus_tag="OO590_004157"
     CDS             complement(1237..2025)
                     /gene="fhuF"
                     /locus_tag="OO590_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6639277.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2166..2402
                     /locus_tag="OO590_004158"
     CDS             2166..2402
                     /locus_tag="OO590_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6639278.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          627 a          532 c          627 g          653 t
ORIGIN      
        1 gtcattgctg atttagccgg tgaaagtgaa gatccacgtt ctgtttgtga acattattat
       61 tctttaatct cacaatatcg ggaaattcac tgggttttca tggtgtcacg ctcctggtat
      121 tcccaggctg tagaactgct catgtgccct acggcgacgt tattgtctga tgttgaaccc
      181 attgagaatc tggtcaagac cgtacgttcc ggcaatacgc atgcagagcg tatcagcgcc
      241 atgctgacct ctccggcaat gactgaaact catgatttta gctatcgctc cgtcattctc
      301 actctttcag agcgcaaggt actgcggcta ttaggtaaag gatggggcat caaccagata
      361 gcttcattgc ttaagaaaag taataaaact atcagcgccc aaaaaaacag tgcgatgcgt
      421 cgactggcaa ttcacagcaa cgctgaaatg tatgcatgga taaatagcgc gcagggtgca
      481 agagaactta acttgccttc tgtttatgga gatgccgcag aatggaacac agccgaatta
      541 agaagagaaa tgtcgcactc atagaaaaat gcgtcatgag tagtatcggt attgagagtt
      601 tattcagaaa gtttgcgggc aacccttata agctccatac ctataccagt caggagtcat
      661 ttcaggatgc catgtcgcgg atctcgtttg cggcggtcat tttttctttt tctgccatga
      721 gaagtgagcg gagagaggga ttatcttgcc tgactgagct ggcgattaag tttccgcgta
      781 cccggcgttt agttattgtg gatgatgata ttgaagctcg gctgattggt tcattgtcgc
      841 catcaccgct ggacggtgta ttaagtaaag cgtcaacgct ggagattttt catcaggaat
      901 tctttttgtc attaaatggt gtacgtcagg cgaccgaccg actgaacaat cagtggtaca
      961 ttaaccaaag ccggacgtta agcccgacgg agagagaaat attgcgcttt atgtcgcgtg
     1021 gctactcaat gacacaaatt gccgagcagc ttaaacgtaa tatcaaaacg atccgagcgc
     1081 ataaatttaa tgtgatgtcg aaactgggcg tcagttctga tgcagggttg ttggaggccg
     1141 cagatattct gttatgtatg cggcattgcg aagcaagtaa tgtgttgcat ccctattaat
     1201 ccgcatgatg ccgggtttac ttccccggca gtgctttcat ttcagcgtac aatagccaca
     1261 ttgctgcaca tccggtaagc gataacgctg gcagcaagtg cggcgcacca gcaggccgtc
     1321 gcgcagtacc acggtacgcc agagtggatt atcttcaccg ttcgtgagcg ttttctcaaa
     1381 aaagagggca tggcgcagcg attcaacagt agcctcgccg agcagttgct tcatctcagt
     1441 gagataccag ttgatcaaat aaccggtatt actccagata agtttgccgt tgatctctcc
     1501 ggtcgcttct agtgcttgca caaccggaac cagcgcctgg ctgattaacg tttccattcg
     1561 ctgctgcggc gaatgtggtg ttgcgttttt atcttcacac acatcgaccc agaaacaggc
     1621 gacgcgtccg gtttcgtgaa actcagcatg gaaatgttcc ggcgacacat ctaatgcttt
     1681 ttcctgcgtc agtagcgcca gcattaatgg tggcaccatc aggccgatat accattgtgc
     1741 ccatagtgag atcagcggtt tgttctcgcg gatcatcgtt ggttggttgc gatagatatg
     1801 atcggaatag accgccagta gagaacttag cgcattcggt gatgaccatt gcgccagcgt
     1861 catggcgtta agcggagcgg gttcatccag gcggataaac tccagcaaat gttcacgatg
     1921 tttggcgatc atcgcccgta cggcttgtgc aagcccagca tcctgcggct ggagatgcgt
     1981 tcgccagatg acatcttcat agagcggtgc ggaacgatag gccataatcg tgatagtaat
     2041 ctaaatgata atgattgcta atcctatcga taggtttact cgatagcaag ggatttctct
     2101 ggcttgcaaa tgataaaaat tatcatatga tattggtttt cattatcaat gaaagagatg
     2161 aaaccatgtt gcaacgtacg ctgggcagtg gctggggagt gttgctgccg ggattgctga
     2221 ttgcagggct gatgtatgcg gatttatcgc cggatcagtg gcggattgtc attctgatgg
     2281 gattagtatt gacgccggtg atgctgtatc acaaacagtt gcggcattac attttgctac
     2341 catcgtgcct ggcgcttatt gctggcatca tgctgatgat aatgaatttg aatcagggat
     2401 gacaaaacag ggaagaaaca agaaaggaag taaagataa
//
LOCUS       DAJTYF010000238         2430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTYF010000238 DAJTYF010000000
VERSION     DAJTYF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.243"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            92..1045
                     /locus_tag="OO590_004159"
                     /pseudo
     CDS             92..1045
                     /locus_tag="OO590_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1086..2081)
                     /gene="pgl"
                     /locus_tag="OO590_004160"
     CDS             complement(1086..2081)
                     /gene="pgl"
                     /locus_tag="OO590_004160"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6639279.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVASYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTATLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          666 a          602 c          550 g          612 t
ORIGIN      
        1 cattttttca ttaatttaag ttggctaaat gcagttaaaa tttttaacga ccagcccccc
       61 aaaatcgtcg cttccatttt ccggagtaat aatgaagcat gaattatcaa gtatgaaggc
      121 atttgtcata ctggcagagt ccagttcatt taacaatgcc gctaaattac tcaatattac
      181 gcaacctgca ttaacgcgaa gaataaaaaa gatggaggaa gatttacata ttcaactatt
      241 tgagcgtaca actcgcaagg ttacgttaac aaaagcagga aaaaggttgc tcccggaggc
      301 gcgggaatta ataaagaaat tcgatgaaac gctttttaat attcgtgata tgaatgctta
      361 tcatcgtggt atggtgacat tagcgtgtat tccgaccgca gtattttatt ttttaccgct
      421 ggcaatcggt aaatttaatg agctgtatcc caatattaaa gtgcggattc tggaacaagg
      481 cacgaataat tgcatggaat cggtgctgtg taacgaatca gactttggca taaacatgaa
      541 caacgtcaca aattcatcca ttgattttac cccactggtc aacgagccat tcgtgctggc
      601 ctgtcggcgt gaccatccat tggccaaaaa gtagcttgta gaatggcagg agttagtggg
      661 ttacaaaatg attggcgtac gttcctccag cggcaaccga ttgttgatag aacaacagct
      721 agccgacaag ccctggaagc tggactggtt ttacgaagtg cgtcatcttt cgacgtcatt
      781 aggactagtg gaggcaggac tggggatttc ggcgcttcct ggtctggcaa tgccccatgc
      841 gccctattct tcgattattg gtatcccact ggtggaaccc gttatacgtc ggacattggg
      901 gattattcgc cgcaaagatg ccgtgctttc tccggcagct gaacgctttt ttgccttgct
      961 gattaatttg tggactgacg ataaagacaa tttgtggacc aatatcgtcg aacgccagag
     1021 acatgcgtta caggagataa gttaatacac cggcgcagga gagactgcgc cggttaaatc
     1081 agcggttagt gtgcgttaac caccacccac attggtccct gcccgaccgc atagcggcct
     1141 ttttcatgca gtagcccctg ctcgccaaca atttcgtata ccgagatgtg gtgagatttt
     1201 tgcccagcgg caatcagata cttgccgctg taatcaacat tgaagccgcg cggctgggtt
     1261 tccgttggct ggaagccttc tttactcaac acgctgccat cttccgaaac gctgaaaacg
     1321 gtaatcagag tggcggtacg gtcgcaggcg tataaatggc gaccatccgg ggtgatatga
     1381 atatcagccg cccaacgggt gtcggagaag ttttccggca tcatatccag cgtctggaca
     1441 cattcgatat tgccgtgcgg atctttcagt tcccagacat ccactgagct gtttaactca
     1501 ttgacgcaat acgcatattg ttcgtttgga tggaatacca tatgacgcgg gccggcccct
     1561 tcaacggtag tcacttccgc agggtcctgc gccaccagat gaccatcatc gctgaccgta
     1621 aacaggcaaa tgcgatcttg ctttaatgcc ggaacccaca gcgtacggtt gtccggtgag
     1681 atattggcgg aatggcaacc gtccagcccc tcgactacat cgacgacgcc cactggcagg
     1741 ccatcttcca gacgcgttac gctcacgtta cccgcattgt aagaagctac aaagacaaac
     1801 tgcccctggt gatcggtgga aatatgcgtc ggactacccg gcagcacaga ctctgcggca
     1861 aaggtcagtg cgccatcgtc cggggcaata cgatacgcca ggacgcgaaa ctcagggcga
     1921 acaccaacat agagataacg tttgtccggg ctgaccacca tcggctgcac ctgccccggc
     1981 acatcgacaa cctgtgtcag cgtcagtgcg ccttcatgat tcaggttcca gacgtgaatt
     2041 tgctggctct cagggctggc gatataaact gtttgcttca tgaacacacc tcaaaaatga
     2101 taaatccctt atctttcata catataacaa cacttactgg ataaaaaaca gacaacaaaa
     2161 cacgataaaa tacaatatcg cacatcaatt aaatcaataa gttagcactc catttcgaat
     2221 ggcatattac taaaatatta gtcaccgaca cgaatttgac aatgccacag atattttcgc
     2281 gactattcag ggagtatgac tctccccgct tttgtttacc tgtatcaacg accgtaaaca
     2341 ctatgccata acaacttctg gccaacttcc cattcgctca acaatcaact cacttccggc
     2401 acaaataaac gcattacgtt gtagatcggt
//
LOCUS       DAJTYF010000239         2380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTYF010000239 DAJTYF010000000
VERSION     DAJTYF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.244"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..388
                     /locus_tag="OO590_004161"
     CDS             <1..388
                     /locus_tag="OO590_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="required for the translocation of capsular
                     polysaccharide through the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6639280.1"
                     /translation="NDDLKVFVMGEVGKQSTLKMDRSGMTLAEALGNAEGMNQDVADA
                     TGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQLEPYDIVYVTTAPLARWNRV
                     ISLLVPTISGVHDLTETSRWIQTWPN"
     gene            376..822
                     /gene="etp"
                     /locus_tag="OO590_004162"
                     /gene_synonym="yccY"
     CDS             376..822
                     /gene="etp"
                     /locus_tag="OO590_004162"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6639281.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            842..>2380
                     /locus_tag="OO590_004163"
     CDS             842..>2380
                     /locus_tag="OO590_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine kinase"
                     /protein_id="HCR6639282.1"
                     /translation="MTTKNMNTPLGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMLPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINTLQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQHQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVE"
BASE COUNT          636 a          566 c          634 g          544 t
ORIGIN      
        1 caacgatgat ctgaaagtct tcgtgatggg tgaagtgggt aaacagagca cgctgaaaat
       61 ggaccgcagc ggtatgaccc tggcagaagc gctgggtaac gctgagggta tgaatcagga
      121 tgtcgctgat gcgacgggga tttttgttat tcgtgcaacg caaaacaagc agaatggcaa
      181 aatcgccaat atttatcagc tgaatgcgaa ggacgcatcg gcaatgatac tcggaacgga
      241 attccagctt gaaccttacg acattgttta tgtcactacc gcgccgctgg ctcgttggaa
      301 ccgtgtgatt tctctgctgg taccgacgat ttccggggtg catgacctga cagagacttc
      361 acggtggatt caaacatggc ccaactaaaa tttaactcaa tcctagtggt ttgtaccggc
      421 aacatttgcc gttcgcccat tggcgagcgc ttgctgcgta aacgtctgcc gggcgtaaaa
      481 gtcaaatcgg caggtgttca tggtctggta aaacaccctg cagatgcgac agcggcagat
      541 gttgccgcta atcatggcgt atctcttgaa ggacatgctg gacgtaagct cactgctgag
      601 atggcacgaa attacgatct gattctggca atggagtcgg aacatattgc tcaggttacc
      661 gcaattgctc ccgaggtccg cgggaagaca atgctgtttg ggcaatggct ggaacagaaa
      721 gagatcccgg atccctatcg taaaagtcag gacgcatttg aacatgtcta cggtatgttg
      781 gagcgcgcca gtcaggaatg ggcgaagcgc ctcagccggt aatttgagtt tataaaatac
      841 gatgacaact aaaaatatga atacgccact aggcagcact caggaaaatg agatcgatct
      901 gcttcgtctg gtcggcgagt tatgggatca ccgtaagttt attatcagcg tgaccgcgtt
      961 attcacgctg atcgctgtcg cttactcgct gttaagcaca ccaatttatc aggcagatac
     1021 tctggtccag gttgagcaaa aacagggcaa cgccattctc agcggcctga gcgatatgct
     1081 ccctaactca tcgcccgagt ctgcaccgga gatccaactg ctgcaatcgc gcatgattct
     1141 cggtaaaacc attgctgaac tgaatctgcg cgacatagtt gagcagaagt attttccgat
     1201 tgtgggtcgc ggctgggcga gattaaccaa agaaaaacca ggtgagctgg cgatcagctg
     1261 gatgcatatt ccacaactga atggtcagga tcagcaactg acactcacgg ttggggaaaa
     1321 cggccactat acactggaag gtgaagagtt caccgtcaat ggtatggtcg gccagcgtct
     1381 ggaaaaagat ggcgttgcgc tgactatcgc ggacattaag gccaaaccag gaacacagtt
     1441 tgtcctgagc cagcgtaccg aactggaagc gattaacaca ttgcaggaaa cctttaccgt
     1501 tagcgaacgc agtaaagaaa gcgggatgct ggaacttacc atgactggtg atgatcccca
     1561 gttgattact cgtattctga acagcatcgc taacaactat ttgcaacaga atatcgctcg
     1621 ccaggcggcg caggattcac aaagccttga attcttacag caccagttac ctgaagtgcg
     1681 cagcgagctg gaccaggcgg aagaaaaact caacgtttat cgccagcagc gcgattcggt
     1741 tgaccttaac ctggaagcca aagccgttct tgagcagatt gtgaacgttg ataatcaact
     1801 caatgagctg accttccgcg aggcagagat ctcccagctg tataagaaag atcacccaac
     1861 ttatcgtgcg ctgctggaaa aacgccagac gctggagcaa gaacgcaaac gcctgaataa
     1921 gcgggtatcg gcaatgcctt ccacccaaca ggaagtgttg cgtttaagtc gtgacgtaga
     1981 agcgggccgt gcggtatatc tgcaattact taaccgccag caggagttga gtatttcgaa
     2041 atccagtgcc attggtaacg tgcggattat cgacccggca gtcactcagc cgcagccagt
     2101 gaaaccgaaa aaagcgttga atgtggtgct tggttttatt cttggcctgt ttatttcggt
     2161 gagtgccgtg ctggcgcgtg cgatgttgcg tcgtggtgta gaagccccgg aacaactgga
     2221 agagcacggc atcagcgttt attccactat cccaatgtcc gagtggctgg ataaacgcac
     2281 ccgtctgcgt aagaaaaatt tattttctaa tcagcagcgc catcgtacta aaaatatccc
     2341 cttcctggcg gtggataacc cggcggattc tgctgtggaa
//
LOCUS       DAJTYF010000240         2241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTYF010000240 DAJTYF010000000
VERSION     DAJTYF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.246"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(155..667)
                     /locus_tag="OO590_004164"
     CDS             complement(155..667)
                     /locus_tag="OO590_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6639283.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="OO590_004165"
                     /pseudo
     CDS             862..1626
                     /locus_tag="OO590_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1577..>2241
                     /locus_tag="OO590_004166"
     CDS             1577..>2241
                     /locus_tag="OO590_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR6639284.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          641 a          488 c          566 g          546 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatgtaa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg ctcgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca acgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggactagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat gatgatgcgg c
//
LOCUS       DAJTYF010000241         2156 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTYF010000241 DAJTYF010000000
VERSION     DAJTYF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.247"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            84..464
                     /locus_tag="OO590_004167"
     CDS             84..464
                     /locus_tag="OO590_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR6639285.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(505..1455)
                     /gene="corA"
                     /locus_tag="OO590_004168"
     CDS             complement(505..1455)
                     /gene="corA"
                     /locus_tag="OO590_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6639286.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            1825..>2156
                     /locus_tag="OO590_004169"
     CDS             1825..>2156
                     /locus_tag="OO590_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639287.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGDIYDESYAPLG
                     LYIENGQR"
BASE COUNT          582 a          506 c          512 g          556 t
ORIGIN      
        1 ttttttgatt tgttattttt ccactttcag tatgctaatg cttgcaccta ctgattcatc
       61 aaaacaatag ttatggagta tttatgagta aggaatatat gaacgatgat tcattaagtg
      121 aaaaatggaa ataccgattt aatttttatg atcaacatgg ctttcctgga ttctggggag
      181 caacaccaga atataaagca gcatttaaag cattaaaagt aagacaacga ttaactattc
      241 aaatgaattt cattgctttc ttctgttcct ggatctattt atttgttctg ggcttatgga
      301 aaaaagcaat tatagttcta ttacttggca ttctatctct atttgttggt gcattaattg
      361 gcgttaacat tctgggaatt gccgtagcag cctatgttgc tgttaacacg aacaaatggt
      421 tttatgagaa agaggtaaaa gggttaaata cctggagcct ttgaataaca ggtggcttag
      481 ccaggctaag ccaccgcctt ctttttacaa ccagttcttc cgcttaaagt acagatacgg
      541 tgccaggccc gcgaggatca taaagataat cgcgccaggg tagccgaaac tccacttcag
      601 ttctggcata aactcaaagt tcatcccgta gctggaagca accagcgttg gcggcaggaa
      661 cacgacggat accaccgaga aaattttgat gatgcggttc tgctcgatgt tgataaaacc
      721 cattgccgcc tgcatcagga agttcacctt ctggaacagg gattcgttat gcggcagcag
      781 ggattcgata tcgcgcagga tttcacgcgc ctgctccagt tgcccacccg gtaaacgcgc
      841 tttacgcacc aggaagttga gagcgcgctg ggtatccatc agacacaggc gaactttcca
      901 gccgatatct tccagttccg ccagcgtgga gagcgcctcg tcgtactcat cgccctgatg
      961 cccttccata atcacccggc tcaactgctc caggtcgcta tagatatttt caatttcatc
     1021 tgccaactgt tcgattttgg tttcgaacag atccagcagc aactcgtagg cgttaccgtc
     1081 taccatcgac tggctacggg cacgcatacg atacagacga aaagcgggca gttcacgctc
     1141 acgcagagta aacagacgac catcacggat ggtaaatgcc acagtggagt taccggcgtg
     1201 atcttccgca tcttcaaaga agaagaagga gtgaatatgc aggccgtcgt cgtcttcaaa
     1261 gaaacgtgcc gatgcttcga tgtcttccag ttccgggcgg gttgccaggc tctggccaag
     1321 ttcagattgt acgcgcagtc gctcgtcgtc gtccggttcg acaagatcaa tccatactgc
     1381 atttacaagg ggttgtgact cttcgacttc cagccgggtc agtcggttat tttccagttg
     1441 aaatgcgctc agcatgaccg ggactcccaa tgcttaaaat atcggacagt tcggtgggca
     1501 cacagaaaca aattgggttt cagaccatta aacagcctga ctcagcgcga cgggaaaaat
     1561 tgaggtcgct gacaaccgct aaggctatca gcaaaaaggg atagccttag gagttgatcc
     1621 tggatgacag gatagtgagc cagtatctac tgggtgtgtc caaggcgaat gtcctcttag
     1681 agtgatcgtg cgcgcatgtt acgccagcaa aattttgccg tcaacacgca acagaacacc
     1741 acagaacaat agttgaactt cgcgcttttt ggtactcgtc aaggtcgcta acaggataaa
     1801 aaaatcccac atccaggacc tataatggcg tatcggctac tcattggtaa aggaatgatc
     1861 accttgaatc tcaaacggat ttttctcacc ctcaccttac ttcccttgtt tgctgttgcc
     1921 gctgatgatt gcgcactctc agatccgacg ctgaccgtac aggcgtatac cgttaatcct
     1981 caaacagagc gggtgaaaat gtactggcaa aaagccaatg gcgaagcgtg gggaacgtta
     2041 catgctctgc tggcggatat taatagtcag ggtcaggtgc agatggcgat gaacggcgac
     2101 atctatgatg aaagctatgc gccgctcggt ttgtacatcg aaaacggtca gcggaa
//
LOCUS       DAJTYF010000242         2141 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTYF010000242 DAJTYF010000000
VERSION     DAJTYF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.248"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(1..211)
                     /locus_tag="OO590_004170"
                     /pseudo
     CDS             complement(1..211)
                     /locus_tag="OO590_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            486..790
                     /locus_tag="OO590_004171"
                     /pseudo
     CDS             486..790
                     /locus_tag="OO590_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            959..>2141
                     /locus_tag="OO590_004172"
     CDS             959..>2141
                     /locus_tag="OO590_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6639288.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          556 a          440 c          490 g          655 t
ORIGIN      
        1 aacctcgatc ggtgtggtgg attagcccgg caggtgggcg ctggctcctg agcgccataa
       61 acagggcttt acccgtcaac tctttcgtca tgcgctctcc catggcgtaa ccgacaattt
      121 cgcacgtata aacatctttg acgccagcga gatacagcca tccttcccgg gtggcaacat
      181 acgttatatc tgcgacccag acctgattag gggggttggg gagcaatgga acagtaaacg
      241 ccgttaagaa gtcaatttaa atagccaaaa attaactagc caatttacat tgcaggttgt
      301 aatttttaac cacctttttt acgatagcca aattacctat acccatcccg gccctgccaa
      361 gccatacccg ctatcggcga tgaaaatgct tttgtgcgcg gtcggagtgg tcccgacgag
      421 ggttttaccc gaagtcgggg cgtatctccg cgttagcggg ccgtgagggc cgcttacgag
      481 cgtgtatgag agacgtacag tggttttgtc cgctcacacc agtacataca ggtacggaaa
      541 taaaaccagt cgaaatgccg gtaacgacga ttactcctgt ctctgatgcc ggtggactac
      601 aagattttat ttactggcgt cctgatgcgg acaggactgg tatggaacct gtttatgtga
      661 tacttaatga tcctctggat tccgggcgat ttacgcgtaa acaactaaat aaaaatatct
      721 taaacatgca agtaattttg gtattctcga tacaagaata aatagtagta actactatag
      781 ttgcaaataa tgttaaaaac atttctatga tcatctttac attttttatg attgacaatt
      841 gtttttggtt aaaaaaagac aactaatgag tgtgataatg caatactttg agtaattata
      901 taatgataag taaacaggtt tgcgacaatt ggttatcagt attacagaag gattaaatat
      961 gtcattattc acagaaccta aggaaattga aagattaccc tctgaagaga ttgaacgatt
     1021 atatcccgtc ctgcgataca gggtctttat cagcattttc ctcggatata tggggtatta
     1081 ttttgtccga aacaccacca gtgtgttatc cggtgtgctg cacatgtctg caaccgagat
     1141 cggcattatc tcctgtgccg gttttttatc ctatggtata tcaaagtttg tttccggact
     1201 tatttctgac agaagtaatt caaaagtatt tttgtctctt ggtttattcc tgtccggact
     1261 ggtaaatttt ctgataggat atattcccgg gatcatcaca tccgttacgt tattttcaac
     1321 aatgtattta cttaacggct ggatccaagg tatgggatat cctcctggtg ccaaaacact
     1381 ggtgttctgg tatgaacatc gggaaaggat cacttgggcc acactgtgga atttatcgca
     1441 caatgtcggg ggagctctgg cccccgttct gattggtttc agttttggtt ttttcggcga
     1501 cagtgctctg gagcatgccc gggccgcatt tatcttccca ggagtactgt gtatggcaat
     1561 gagtgtgctg atttacttta ttcaggtgga cagacctgta tcagtggggc ttcctcccat
     1621 tgaagaatgg aaaggtaacg tcgtttcaca cccggctaaa ggccgtgaac aaggtccgag
     1681 actctccatt cctgatatca tcagaaaaca tatcatccga aataataaac tcatttactg
     1741 ctgtatctat ggttcgtttg tttatattct gagatacggt attgtttcct gggctcctaa
     1801 gtttttaagt gacagtctgg atgtcggtgg taaggatatg ggaaaactgg catcaatggg
     1861 ggggttttct gtttttgaaa tcggtggtgt ggctggtatg cttcttgccg gatacctgtc
     1921 tgtcagactg ttcagaaact caaaaccatt aacaaatact ttatttctgg cacttaccat
     1981 tattttatta atcgcgtact ggtacgtgcc atcaggcaat gaatatctct ggctaaacta
     2041 tacaatcctt attctgttgg ggctggcagt gtatggtccg gtgatgttta ttggtcttta
     2101 ttcaatggag cttgttccta aagaagctgc cggagccgca t
//
LOCUS       DAJTYF010000243         2129 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTYF010000243 DAJTYF010000000
VERSION     DAJTYF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.249"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(279..461)
                     /locus_tag="OO590_004173"
     CDS             complement(279..461)
                     /locus_tag="OO590_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639289.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNSEDN
                     DEAASDYALKINESSD"
     gene            792..1664
                     /locus_tag="OO590_004174"
     CDS             792..1664
                     /locus_tag="OO590_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR6639290.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHWGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1882..>2129
                     /locus_tag="OO590_004175"
     CDS             1882..>2129
                     /locus_tag="OO590_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6639291.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKST"
BASE COUNT          547 a          504 c          506 g          572 t
ORIGIN      
        1 gtatctggct cgttgccggt gttaccgata tgtgtaaatc tttcaacgga tacgacatat
       61 gctgaaggag gatctgtaat ggattaagtg ataacagata ctcagaaata taggggcaaa
      121 tccactattt tgatgagaat tattatatgt tacctcggat tttaatcagg cgccaagatt
      181 aaataaaata ttgagcgcct gattatattt attcattcat tcattcattc attaattaat
      241 atctgttatt gattggctta ctatcagctt cccccgtatt aatcagagct ttcatttatt
      301 tttagtgcat aatcagatgc cgcctcatca ttatcctcac tattatcata gccgaaaact
      361 tcccccggac acgggcgttc acgataatag tcatcgtcat agttttcatc gtcagataat
      421 gatatgtcgt attctgaata tgattcacca caattataca tgaaatcatc tcctttgggg
      481 tggttacaat aattagtgag tttattcgaa aaaatgatga gcgccagacc aacccaccca
      541 acttttgatt ataatcatga agatacttgt ctcagtacaa atagaatcaa actcagtgca
      601 gcatgattca cctttatatt ctctcaggga aaccaaatac cactactcaa ctattaagaa
      661 caaaattctg gaggtatgat tatctgaaga tacctgtatc ctaaataatt tcagacagat
      721 attatcgttg tgagtccccc gccacctttc cggtgcgcgg ggttttgtct tttttcaccg
      781 ggaatacatg tatgaatccg tctgatgcca ttgaggcaat tgaaaaaccg ctctcctccc
      841 tgccttactc actttcccgt cacatcctgg aacatctgcg caaactcacc agtcacgaac
      901 ccgtgattgg cattatgggt aaaagcgggg ccggtaaatc ctcactctgt aatgcactgt
      961 ttcaggggga ggtcaccccg gtcagtgatg ttcacgccgg cacccgggaa gtacggcgct
     1021 tccgtctgag tggccatggt cacagcatgg ttatcactga cctgcccggg gtgggcgaga
     1081 gccgggacag ggatgcagag tatgaggccc tgtaccgtga cattctgcct gaactggacc
     1141 tggtactgtg gctgattaaa gccgatgacc gtgccctgtc tgtggatgag tatttctggc
     1201 gacacatcct gcactgggga catcagcggg tactgtttgt ggtgacgcag gccgacaaaa
     1261 cggagccctg ccatgaatgg gatatggccg gtattcagcc ttctcctgca caggcacaga
     1321 atattcgcga aaaaacggag gcggtattcc gtctgttccg gcccgtacat ccggttgtgg
     1381 ccgtatcggc ccgcactggc tgggaactgg atacgctggt cagtgcgctc atgacagcgc
     1441 ttcccgacca tgccgccagt cccctgatga cccgcctgca ggacgggctg cgcacggaat
     1501 ctgtccgggg tcaggcccgt gaacagttta ccggtgcggt ggaccggata tttgacacgg
     1561 cggagagcgt ctgtgttgcc tctgttgccc gtaaggttct gcgcgctgtt cgtgacaccg
     1621 tggtctctgt tgcccgcgcg gtatggaact ggattttctt ctgaacccgg tggtgatgca
     1681 ccagactctt gttacagatt tttaacccca tgaaaacaca atgcaaacac agccataccc
     1741 gggttgtccc cttgcgttgt gtttctcaag ttaatagaat gcatcgatcg ttactatcat
     1801 atcgatcgct ataaacgatc gtttgtggta aatgacctcc tccgtggttg tcatgaaaat
     1861 gcgtctcagg aaggttaaat aatgcacgaa aagaacatcg ccctgctttg tgatgaagcc
     1921 gaccgacttt tgcaactgaa cattaatctg ctccggcaaa tggttgatga accggatgtg
     1981 ttatctgaca gtaagaacga aaacagactg ctttttgata aacagaaagc actgaaaaga
     2041 attgaggagc tggagggcga acaaatcaaa accgcccgca gggagatggt gctggctgtt
     2101 gtcggcacga tgaaagcagg taaatcaac
//
LOCUS       DAJTYF010000244         2120 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTYF010000244 DAJTYF010000000
VERSION     DAJTYF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.250"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            20..1072
                     /locus_tag="OO590_004176"
     CDS             20..1072
                     /locus_tag="OO590_004176"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6639292.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1148..2120
                     /locus_tag="OO590_004177"
                     /pseudo
     CDS             1148..2120
                     /locus_tag="OO590_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
BASE COUNT          510 a          482 c          557 g          571 t
ORIGIN      
        1 ttaaattgag ggcattatta tgaaaaaaat accctgcgtg atgatgcgag gtggaacctc
       61 gaggggcgcg ttcctgttag cggaacattt acccgaagat caaacgcagc gcgataaaat
      121 attgatggca attatgggtt ccggtaacga tctggaaatt gacggtattg gcggcggtaa
      181 tccactgacc agtaaagtcg ccattattag ccgttccagc gatccgcgtg ctgatgtcga
      241 ttatctgttt gctcaagtta tcgtccatga gcaacgtgtc gataccacgc ctaactgcgg
      301 caatatgctg tctggcgtag gggcattcgc cattgaaaat ggtttgattg cagcgacttc
      361 gccagttacc cgtgtacgta tccgcaacgt caatacgggt acgttcatcg aagctgatgt
      421 gcaaacgcca aatggtgttg tcgagtacga gggtagcgcc agaattgacg gcgtaccggg
      481 tactgccgca ccggttgcgc tcactttcct gaatgccgct ggaacgaaaa ccggaaaagt
      541 tttcccgact gataatcaga ttgattattt tgacgatgtc ccggtgacct gtatcgatat
      601 ggcgatgcca gtcgtcatta ttccggctga atatctggga aaaacaggtt atgaattacc
      661 ggcggaactg gatgccgaca aagcattatt agcccgcatt gaatctatcc ggctacaagc
      721 gggtaaagca atgggcttag gtgatgtcag taatatggtt atccctaaac ctgtgcttat
      781 ttctccagcg cagaaaggcg gggcaattaa tgtgcgttat tttatgccgc attcttgcca
      841 tcgcgcgctg gcgataaccg gtgctattgc tatttccagt agttgtgcat tggaaggcac
      901 cgtcacccga caaatcgtcc cttctgtagg atatggcaat atcaatattg aacaccccag
      961 tggtgcgctc gacgttcatt taagtaatga aggtcaggat gccacgacgt tacgcgcatc
     1021 tgttattcgg acaaccagaa aaatattttc cggtgaagtt tatcttccct gaaaaaattc
     1081 gttgtcagga taaggacaat caataaagga cttctgtatg agtcatacag aaagaacagg
     1141 attttaaatg aataagaaat cgttatggaa gctaattctg atattagcga tcccatgtat
     1201 tattggtttt atgccagctc cggcaggatt aagcgaactg gcgtgggtgc tttttggtat
     1261 ttacctggcg gccattgtgg gtctggttat caagcctttc ccggaacctg tcgtactgtt
     1321 aattgccgtt gctgcctcaa tggtggtggt cggtaactta tccgacggtg cgtttaagac
     1381 caccgccgta ttaagcggtt actcttcagg tactacctgg ctggtgttct cggcgtttac
     1441 cttaagcgcc gcatttgtga ccaccggttt aggtaaacgt attgcctatc tgctgattgg
     1501 taaaatcggt aacaccatgc tgggtctggg ttacgttacg gtattcctcg atctggtact
     1561 ggctccggca acaccgtcta acaccgcgcg tgcgggcggc attgtgttac cgatcatcaa
     1621 cagcgtggcg gtggctttgg ggtccgaacc ggaaaaaagt ccgcgtcgtg tcggacatta
     1681 cctgatgatg tccatttaca tggtcaccaa aaccaccagc tatatgttct ttaccgcaat
     1741 ggcggggaac attctggcgc tgaaaatgat caacgacatt ctgcacctgc aaattagctg
     1801 gggtggatgg gcgctggccg ccggattgcc ggattgccgg attgccggat tgccggattg
     1861 ccgggcatca ttatgctgct ggtcaccccg ctggtaattt acaccatgta tccaccagaa
     1921 attaaaaagg tggataacaa aaccatctct aaagcgggcc ttgccgaact gggaccgatg
     1981 aaaatccgcg aaaaaatgct gctcggtgtc tttgtgctgg cgctactggg ctggattttc
     2041 agtaagtctc tgggggttga tgaatccact gtggcaatcg ttgttatggc gaccatgctg
     2101 ctgctgggta tcgttacctg
//
LOCUS       DAJTYF010000245         2094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTYF010000245 DAJTYF010000000
VERSION     DAJTYF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.251"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..147)
                     /locus_tag="OO590_004178"
     CDS             complement(<1..147)
                     /locus_tag="OO590_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6639293.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFK"
     gene            complement(1265..1777)
                     /gene="clbS"
                     /locus_tag="OO590_004179"
     CDS             complement(1265..1777)
                     /gene="clbS"
                     /locus_tag="OO590_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin self-protection protein ClbS"
                     /protein_id="HCR6639294.1"
                     /translation="MAVPSSKEELIKAINSNFSLLNKKLESITPQLAFEPLLEGHAKG
                     TTISVANLVSYLIGWGELVLHWHDQEAKGKTIIFPEEGFKWNELGRLAQKFYRDYEDI
                     TEYEVLLARLKENKQQLVALIERFSNDKLYGKPWYNKWTRGRMIQFNTASPYKNASGR
                     LNKLQKCLAE"
     gene            complement(1812..>2094)
                     /gene="clbQ"
                     /locus_tag="OO590_004180"
     CDS             complement(1812..>2094)
                     /gene="clbQ"
                     /locus_tag="OO590_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colibactin biosynthesis thioesterase ClbQ"
                     /protein_id="HCR6639295.1"
                     /translation="MTIFTPIIKNDYRLYEQYVFQAKARTLTCPIVLFHGDADNLVMQ
                     DELLAWEKFTTRKTRTIIFPAADHFFVDKHFEQVVGYVNQTIESLEIVG"
BASE COUNT          597 a          482 c          411 g          604 t
ORIGIN      
        1 tttgaaaata acattatcat tgacgccatt ttcacgtgca atctgtgcaa cacaagctcc
       61 aggttgtgat gccagttcca ccatacgaag tttgaattca ttcgaatagt ttttacgagg
      121 ttcttttcgc cagtcctgta attccatact tagatgtccg tctatatcag atgggcgtct
      181 aagttaccaa ttctcgtctg atggctacat acggcggtca gtttacgctt actacaatga
      241 tgataatgcg attatataaa ggtcaaacca tatggttgtt agcggatttc aacaacggtg
      301 ctgtactcgc tgagcggcac atgaagtttg aataatgtgg aaacaacaaa tggctacatt
      361 aagtcattgg acatatacag agatggttgg caaatcgagt acacgatatg ttgcctgaaa
      421 agtcgaccga acagaactat aaatatcaag tcggcataac acatctaaat ttcataaggg
      481 aagatgtgcc accggggacg tttttttcac cacggggact tcgggtataa cgatgcaatc
      541 cgaagaaaga aatccaatgt tggcgatatt catcatcgct ttattcgtcc actcatgcga
      601 atggcaataa agcgactcac caaaacagtt tctttagctt ccctctacca aaattcccta
      661 tgatcgcatg ttagaactct gcgtgctgaa cttcgggcca tatggacggt atcattcatg
      721 cacttgctcc tttgaaattg ctggatatca ttcgtcttta tctgcaccgg ccggcacaaa
      781 tagctgtagg ggaacccggc gcaggcaaaa agagcgactt taacgcgttt tgcaggactc
      841 cccgttgcct tgcagggaga cactgtatca tcaaacacca tcttaatccc agtacattac
      901 cctccatcct ggctcgtacc aaatcagagg aaaggcctcg cggcaataga ttttcccggc
      961 gaagctcgga gtgaaataca gtgcagcaat atccgacatc agaatttgat cctcctgcgt
     1021 caggatacga aaaaacatcc cgatttgagc acccttgtcg aaaaaaaatc accttattat
     1081 gaagattggt tcttaataca catgcgttcg catgtgctta tgttaagtag acttattgtc
     1141 ttacacattt tatccgtatc attaaccaga atatgttccg gagcagcggg tgatagtgcc
     1201 gccagtccac actcatcgct gcccggaaaa atcatcagtg gggaggcaaa cggtaagcac
     1261 cccgctattc tgcaagacat ttctgcagtt tatttaacct ccccgaagca tttttataag
     1321 gcgaggcggt attaaattga atcatacgac ctcgggtcca tttattatac caaggtttac
     1381 cgtaaagctt gtcgttactg aatcgttcaa tcaaagccac gagttgctgc ttattttcct
     1441 ttaaccgtgc caataaaact tcgtactctg taatatcctc atagtcacgg tagaatttct
     1501 gtgctaaacg ccccaattca ttccatttaa atccttcctc aggaaaaata atagtttttc
     1561 cttttgcctc ttggtcatgc cagtgtaaca ccagctctcc ccagccaatc agataggaaa
     1621 ccagattcgc tacgctaatc gtagtcccct tcgcgtgccc ttccaataga ggttcaaagg
     1681 cgagttgggg cgtaatagat tctagcttct tatttaataa agaaaaatta ctattaatag
     1741 ctttaattaa ctcttctttt gatgatggaa cagccatatc tattgctcct tgtatagtta
     1801 cacaactatt tctaccctac tatttcgagt gattcaatcg tctggttcac ataacctacc
     1861 acctgttcaa aatgcttatc gacaaaaaaa tgatcggcag caggaaatat aatagtccgc
     1921 gtctttcgtg tggtgaattt ttcccatgca agtaattcat cctgcattac cagattgtca
     1981 gcatcgccat gaaatagcac gatcggacag gttaatgtgc gcgccttggc ctgaaataca
     2041 tactgctcat agagccgata atcgttttta atgatggggg tgaaaattgt catt
//
LOCUS       DAJTYF010000246         2089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTYF010000246 DAJTYF010000000
VERSION     DAJTYF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.252"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(60..1025)
                     /locus_tag="OO590_004181"
     CDS             complement(60..1025)
                     /locus_tag="OO590_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR6639296.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="OO590_004182"
     CDS             complement(1879..>2089)
                     /locus_tag="OO590_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639297.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVRIDLNGIKFGFLRTHS"
BASE COUNT          577 a          551 c          436 g          525 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcataac gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatacccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggga taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattctc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aattaccgct tcacttctt
//
LOCUS       DAJTYF010000247         2083 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTYF010000247 DAJTYF010000000
VERSION     DAJTYF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.253"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..270
                     /locus_tag="OO590_004183"
     CDS             <1..270
                     /locus_tag="OO590_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6639298.1"
                     /translation="FGMVLAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAW
                     MCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAKQK"
     gene            275..1225
                     /gene="virK"
                     /locus_tag="OO590_004184"
     CDS             275..1225
                     /gene="virK"
                     /locus_tag="OO590_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6639299.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1290..>2083
                     /gene="lpxM"
                     /locus_tag="OO590_004185"
                     /gene_synonym="msbB"
     CDS             1290..>2083
                     /gene="lpxM"
                     /locus_tag="OO590_004185"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6639300.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPM"
BASE COUNT          602 a          396 c          468 g          617 t
ORIGIN      
        1 tttggtatgg tgctggcaga agcatcggca ttttctgtgc ctgtagtggc cactcagatt
       61 ggtggaatcc ctgaggttat tcagaacaac cagaccggga cattgttacc agcaagtaat
      121 aagcacgcat ggatgtgcgc cctgaatgat ttttttaatg accctgggcg tttttatcag
      181 atggctcgcc tggcaaaaca ggatatagaa gagcggtttg atattaataa aactgcgtta
      241 aaaatactca cattagcgaa gcaaaagtaa caatatgttt tctataagta acttatcatt
      301 tatcggtttc cttaaaagga ttattttttc ctcagattca ctaccgggga agtgggaaca
      361 cagaaaattt cggttcatgt acattttgcg atgttctata aatccggttg tcagtattcg
      421 atattattat gaactgcgtt ccttgccgtg cattgaggat attctggcaa tacaccccac
      481 gttgccagca agaattcatc gcccttattt gcataaaggg gggcgcgcct ggacccgtgg
      541 acaatatatt cttgagcatt atcgctttgt gcaaaatttg ccagaaaaat attctaaatt
      601 tttgtttcca caaaaatcgg tatcattagt ccaattcatc gggaaagatg gagagaattt
      661 tgatatccag tgttctccca gtggttttga ccgggaagga gagttgatgt tgtcattgtt
      721 ttacaataaa acagttattg cccggttaac attttcagtc attctgactc aaaatggaca
      781 cattgctttt attggcggat tacagggagc ccctaaaaat accggacctg atgttattcg
      841 gtgcgcaacc cgggcctgtt atgggatttt cccaaaacgg attatttttg aagcattttg
      901 tgcgttgatg aaagcttgca atatctctga gtgtctggca gtcagtgagc acagtcatgt
      961 tttccggcaa ttgagatact ggtatcagaa acgcaaaacc tttgttgcgg tctatagtga
     1021 tttctgggag tctgtagcag gaaaaacctg tggtgactgg tacagattac caacccaggt
     1081 gattcgaaag ccgctaagcg atatagccag taaaaaacgc tctgagtacc gaaaacgata
     1141 cgctttgctg gattatatcc atgaaactac tatccgctct ttggatgcat atcctgtaca
     1201 ctcagaacat cagaatttaa attaaggttt gatgtattct ctgaataaaa cagtaatgat
     1261 aattttggta gaggcattcg cactaaataa tgaaaaaata taaatctgag tttattcctg
     1321 aatttaagaa aaattacctt tcccctgttt actggtctac atggttcctt ttgggaatga
     1381 ttgcaggtat ttcaatgttt cccccttcat tcagagatcc tgtcttggca aaaatagggc
     1441 gttgggcggg cagattgagc aaaaaagctc gtcgcagggc gacgattaat ttatcgcttt
     1501 gtttcccaga aaagagtgat acagaacggg aaataattgt cgacaaaatg tttgtcacag
     1561 cattgcaatc catagtgatg atggcagaac tggcaattcg tggtccggaa aagttccaga
     1621 aacgagtgtt ctggaaaggc ctcgaaattc ttgaggaaat tcgacacaat aatagaaatg
     1681 tgatttttct ggttccccat ggctggagcg tggatattcc tgcaatgttg ctggcagctc
     1741 agggaaaaaa aatggccgcc atgtttcatc agcaacgaaa tccagtgatt gattatgtct
     1801 ggaattcagt acggcgtaaa ttcggggggc gcttacatgc ccgggaggat gggataaaac
     1861 catttattca gtcagtacgc cagggatact ggggatatta cctcccagat caggatcatg
     1921 gtcctgaata cagtgaattt gctgattttt ttgcgaccta taaagcgaca ttaccaatca
     1981 ttggacgtct gatgaacatc agtcaggcta tgattatacc acttttcccg gtttatgatg
     2041 aaaaaaaaca tctactgact attgaaattc ggccaccaat gga
//
LOCUS       DAJTYF010000248         2081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTYF010000248 DAJTYF010000000
VERSION     DAJTYF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.254"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..164
                     /locus_tag="OO590_004186"
                     /pseudo
     CDS             1..164
                     /locus_tag="OO590_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            351..588
                     /locus_tag="OO590_004187"
                     /pseudo
     CDS             351..588
                     /locus_tag="OO590_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1009..1587
                     /locus_tag="OO590_004188"
     CDS             1009..1587
                     /locus_tag="OO590_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639301.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1696..1954)
                     /locus_tag="OO590_004189"
                     /pseudo
     CDS             complement(1696..1954)
                     /locus_tag="OO590_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1938..2081
                     /locus_tag="OO590_004190"
                     /pseudo
     CDS             1938..2081
                     /locus_tag="OO590_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          659 a          364 c          505 g          553 t
ORIGIN      
        1 aaatcacaag gagagggatg tatcagcagt gactgaaggt tgacccgctg aagtgcgagc
       61 tgcgcggagg tcagatgcgg tttacggggc tgaagagggg ttaccgtctg gcagagcagg
      121 ttatgatgca tgagccgctg gctcgaatac ggtggtgcgg ctgagagtcg cataggggaa
      181 gttgcgtcca tgttgagggg aacggagcaa aaaaccatca gagatacatt gaatcaatca
      241 atgtaagcaa tattaagtgt ctataccgat tacttgatga tcaggttgtg ttgaataaac
      301 gcgcattctt cggtgctttt ttaatctttt ggggatttaa attcctatcg atgaactgtc
      361 gatatgaaaa agcgagcatc atactgacgt caaacaaagg ggtcgcagac tggggagaaa
      421 tgttcggaga tcacgtgctg gcaacagcga tcctagatcg gctgctacac gctgaatatc
      481 aaaggagaga gttaccggtt aaaagagaaa cgtaaagctg gagtgctgac caaaaacaca
      541 acgccaatca gtgatgatga aatggtggaa aacggacagc atcagtaacg aaagtatctt
      601 agcaggcatg aaaatcgtcc gcatccccta aacggtcaaa atgcttctgc cgtgaaggag
      661 ggagaatgcc atctcatcac ttatatctgg cgaatgaaac acagatggtc ggccttgccc
      721 ccaaacacac ggcggtgcaa aatggaatgg cagaaaactt cgtcaaaacg atgaaatatg
      781 attaaatcag tgttatgttc gaacctgacg tgttaacagc agtaaagtag tctcgcgaag
      841 gtgttcaaac attataatga atggcatcct catagtgcgc agggttatcg cttgctacgg
      901 aaatatctga agcgacgtac cagtaatggg gaaaatgata gctggtttct gaaaatatag
      961 aggaaaatgt gatatatata tcctgttaat atttaaggag aatataaaat gaaagtctcg
     1021 tttaagtctt taggctatat ttttcatgat atatataata aaaaacacac tatcgatgag
     1081 ttcaacgatg tggtaagaaa agcagtttta tcaggtaaaa ttaacgagct gaatgcatgt
     1141 cataaagtgg caatttttct tgccgaaaaa gacaatgaga taacaaagaa agacaaagcc
     1201 aagataatag atacattaac tgaaaactat tccattgaat ttcaacaatt aatgaatatt
     1261 agtgagcgca cgttaaattc atctttatat attactcctg gtgaaagtgg ttttgtcagt
     1321 tttgttaatc gtgaaggaaa aatatgtcat actgcatatg taaaatcatc agataatagc
     1381 atggcatatt accatgcaaa tggtagcagc attgacaaat atattacgga tatgtgcggc
     1441 cttatttgta tgaggcatat tgagtctact ggcattattt tttatatgct tgatgaaaaa
     1501 gttttatctg ccattgctga atttatgaat gaaaaaggat ggcgggctgc gttttgttca
     1561 gcaaagaact tatataaatg tgtttaattt tagaaaaact atttggtaaa atccaacaag
     1621 agtcgagagg ttcgcgttgc ttcagcaggg cctcagcctg ttcaaccaga ctcagttgca
     1681 ggtcgtgacg cagatttagt ggtggtggtt gattgtcaga taccagccga tgatcttgta
     1741 gtgcatatca tcagactttg agaagcatat ggttttgcgg gtaagccgct taagatgtgt
     1801 ccgcaggttc agattatgtc gctctatccg ctgagtgcat ttcttgctta taacatgact
     1861 ggttgaggac aataaagctc ggtataccgg ctcaggcatc ggtcatgtag aaggcaatgc
     1921 taaactggct cagcaaacta agagcagcgc gcatccactt acccaatgcg gttgagaaaa
     1981 tagccttcga tcgctttcat gtggcgaaac aaccgggtga ggtggtggat aaaacccgcc
     2041 agaatgaaca tccgcatctt cctattgaaa gtcgtcgtca g
//
LOCUS       DAJTYF010000249         2077 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTYF010000249 DAJTYF010000000
VERSION     DAJTYF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.255"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(195..392)
                     /locus_tag="OO590_004191"
                     /pseudo
     CDS             complement(195..392)
                     /locus_tag="OO590_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(524..2047)
                     /locus_tag="OO590_004192"
     CDS             complement(524..2047)
                     /locus_tag="OO590_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6639302.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          622 a          459 c          442 g          554 t
ORIGIN      
        1 acatcaaaat tattaatagt tgccatggta attatttcgg tgatatataa aaacaactgt
       61 gataacacca aggttgacaa ctatttgcgc aataattcct ttccttcaat gatccaggcg
      121 ttcactgcca gcaacccatt cagccgcata ctgacagtga gaaactgaaa gattattgct
      181 gatccctgtg gctgacgagt ctttctctta aatcgattaa ccagattgat cgccagccaa
      241 acaagggcat taagcaaatt tatcgtcaca agcacttctc ccagcccggt actgggtttt
      301 gcaataagga tgaccaggag ggtgatcagc ataaatgcaa tggaaaagat gatatagaga
      361 gtgaaataat ccagattatt actattgttc atgctactaa tggcaatcct gtcaaaactt
      421 tcaaacaatt atagcaaaca aaataatgaa caatagtaac aagaggcaac gagctaatta
      481 gctgtctata attacaccgc actatggcct tactgatcaa ttttcactca accacaacct
      541 tcaccttcgg tttagaaagg ataattttga ccaggtcgat gtatgtaaca ggtttataga
      601 aataataacc ctgctgaaat gtgatattat tttggttcag atagccaagt tgttctttcg
      661 tttcgacacc ttcagcgacg atactgatcg aaagtttacg cgccagatcc aatacacaat
      721 ccagaattcg ggttgattct gggtctgcgt ttacgcggcc aacgaaactg tgatcaattt
      781 tgatataatc aatatgcagg tcatgaagat aagaaagccc tgagtagcca gtaccgaaat
      841 catccagcgc gatgacaaaa ccattttcat gcagaatgtt caaccgctga accagacttt
      901 catcaacatt caatggctca cgctcggtga cttccagaac aaggtttaaa tcgcggcggg
      961 taaaactgtc acggaaattt aaacactcgt cgacaaacgt cggcgaaata atatgcgaag
     1021 cgctaaaatt aattccaata tgaaaacctt ccggcagttt actcgcgata gcgttcatct
     1081 gtctggcaac ctgattcatc aggctttgtg taagcggaac gattaatccg gatttttcag
     1141 caagtggaat aaatgccgcg ggtgaaatat atccaccgtg aggttgtttc cagcgggcta
     1201 acacctcaac tccccgcaat gtcccttccc gaccatttac cacaggttgg taaaaaggga
     1261 cgatctcccc attatttatt gcccgtcgca ggatctcttc aggcgttgcg cttttattga
     1321 aatatctatc aagcaggaag gcagcggcgc aggcgattaa cagaataaat attaaaatcc
     1381 caaagccttt atcgataagt ctgtagaagc taaagagtgg cggcgggtta aagataatag
     1441 tgaaatgata ttttgtggag tgaaccttct cggtataggg ataatgtcca ttaagcgtca
     1501 tcacatcacc cgttggtcca ataatgtcat ccgccacgcg taatacatac cttacccctt
     1561 tcaagggtac attaagcgcc ccgcgaatat gttgatcact tatcgtaacc aaaatgcgcg
     1621 tatctgcaaa ttggctctga tagagcagga taggtaatct attaacggtg tcgattgccg
     1681 gagccaacag taaattactg tccgggacaa caggaatacg agacagcagg acccgattcc
     1741 caggcaggga tgtacaccag actattccct gtttgagaat gatgattgtg cgcaggtgag
     1801 gtttcagagc tgcttcagtg ccaagctgat attgctcctc gaggtcgcat tccttcccgg
     1861 caatgttaac cgctgtttga gtcgcctgtg acgcttcttc aagtatatga ttgatattgt
     1921 tggcggcata tcttgctccc gccaggtact ctgcttttgc ggaataccag agctggatgt
     1981 tcaaaatagc gacaccggta ataaacaagc atatcgcaac gaggatgggt atgagtgtat
     2041 tgcgcatttg cttgtcccgg ccaaagggtt taataca
//
LOCUS       DAJTYF010000250         2071 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTYF010000250 DAJTYF010000000
VERSION     DAJTYF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.256"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..2067
                     /locus_tag="OO590_004193"
     CDS             <1..2067
                     /locus_tag="OO590_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6639303.1"
                     /translation="LGVPGMPATTKGIRQALQRFSRDLGDVSRRREGTKAIEYHIDCL
                     PEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGSPQLMEERLNA
                     LTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPERLQQAAVNAN
                     AKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEISWLPEFLAQYR
                     QVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEVVKQKGRVTGS
                     EYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTFVIDGGTRFVV
                     GWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVVGILPRLGIKH
                     PTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRDLRSAFNALQQ
                     DKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRHYSPTEFRKKR
                     LAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDEHGRKVLISYD
                     IHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRKRAEEKIRDAE
                     DEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRLGMQKLFAMQE
                     EDDPLA"
BASE COUNT          556 a          498 c          590 g          427 t
ORIGIN      
        1 ctcggcgtac caggaatgcc agccaccaca aaaggcatcc gacaggcgtt acagcgtttt
       61 tctcgtgatc ttggtgacgt tagtcgccgt cgggaaggca caaaagccat tgagtaccac
      121 attgattgtc tgcctgaaat aacacgaaag gcactgcgtg aacgctatgt ggaacagctg
      181 gtggctacag agaacaatgt ttctgaagtg aaagctgtta ccagaaaaac acgcaatcct
      241 gacgctgtcc aggcaatcga agcatatcgc ggttctccac aactgatgga agaacgcctg
      301 aatgcgctga ccgaaaacca gcgctgggta tctgaagcaa gagctgcgct ggtggtggaa
      361 gtgctgaagc tggaaagcgc cggtaacctc gggcgactga aagccattaa ctttcttgtt
      421 gaaaaagccc gtaaaggtga gctgccggag cgcctgcaac aggccgcagt taacgccaat
      481 gcaaaacgtg gcgctaatcg tactatcagc cgcgatccac tttaccagtg ggttctgaaa
      541 tacaaccagt cgcagaacgc cgccgaacgg ctgctgttac tggcaccggg aaaacgcgac
      601 gaaataaaac cggaagaaat cagctggctg cctgaatttc tggcgcagta tcgccaggtg
      661 aacggcagac ccatgtcaga agcctacgag gattttgtcg cagaatggca gcgtcgccat
      721 gcggacgaac cgtacatgct ggaggtgatg ccttcctatg atgttgtccg gtatgccatg
      781 aaaaaactgc cggaagtggt gaaacaaaaa gggcgcgtta ccggcagtga ataccgtcag
      841 cttgaaggat tcacccgccg cgactggaca gctatgccgg tgaattatgt ctggattggt
      901 gacggtcacg gtatgaagct gaaatgtgcg catccgatcc acgggcgtcc attctcaccg
      961 gaagtcacgt ttgtgatcga tggtggaaca cggtttgttg ttggctggag tcttgacctt
     1021 gccgaaaacg tgttcgccgt tgccggtgcg atacagcacg gcattcgcaa ccacggaaaa
     1081 ccgttcctgt attactcgga taacggttcc ggtgaaacgg ctgacatgct ggacaaagag
     1141 gtcgtgggta ttctgccacg cctggggatt aaacacccta ccggtattgc cggtaatcca
     1201 cagggacgcg gcattattga acgcctgaac cggacactgc cgatgcgtat agccaggagg
     1261 tatcgcacct attttggcaa gggtgctgac cgggaatcgt tgcgtgtact taaccgtgat
     1321 ctgcgttcag cgtttaacgc cctgcaacag gacaagcctc tgaacgaccg ccagaaagcg
     1381 gcaatgcgcg agctgccatc gtgggcggag cttattgagg ctattcgtga aggcgtggaa
     1441 tggtacaaca accgcccgca ttctgagctg ccgatgaaac cgaacggacg gcattacagc
     1501 ccgacagagt tcagaaaaaa acgcctggca gaagaagaca ccgaaattga gtggctgagt
     1561 gatcttgagc tgcgcgacat gttccgccca atggtggaac gaccggtaag acgctgcgaa
     1621 attcagtggc tgaacaatat ttactacgcg ccggagttgc gtgacgaaca cggtcgcaaa
     1681 gtgcttatca gttatgacat tcatgacgcc gagcgaatta cggtacgtcg caaggacggc
     1741 agctttatct gtgaggcgat atggaacggc aataaacgcg cagcctttgc tgtcagtgct
     1801 gaatatcaca aacaacagca gcgtattaaa ggaatgcgca aacgcgctga ggaaaaaatt
     1861 cgtgatgctg aagatgaagg tattcagatt ctggaacaca aacaggctga accctggctg
     1921 agtaatgttt atcgcccggt tggtaatgtg gtggcagttc agcaaccgga atatgaagaa
     1981 gagcatgacg aagaatttga acgcgatttc cggctgggga tgcaaaaact ttttgccatg
     2041 caggaagaag atgatccgct ggcctgaata a
//
LOCUS       DAJTYF010000251         2061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTYF010000251 DAJTYF010000000
VERSION     DAJTYF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.257"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..168
                     /locus_tag="OO590_004194"
     CDS             <1..168
                     /locus_tag="OO590_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR6639304.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGIL"
     gene            153..791
                     /locus_tag="OO590_004195"
     CDS             153..791
                     /locus_tag="OO590_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6639305.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..1420
                     /locus_tag="OO590_004196"
     CDS             1019..1420
                     /locus_tag="OO590_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639306.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEISLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSSGGGR"
     gene            1431..1905
                     /locus_tag="OO590_004197"
                     /pseudo
     CDS             1431..1905
                     /locus_tag="OO590_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          625 a          369 c          481 g          586 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgattttt ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ttctgtgaca ccggagatac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat acaggaaaac gcctgagggg ctggttgcct gtagcctgta
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa aatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatctctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa aatttccaaa gctcagctta tatctcccga
     1381 ttatgttttg attgattttt ctagtggggg ggggagatga accctatgca atgtcctggt
     1441 taatatctca gtgtgctcat cagtgcactg ataataaaaa gacggagaca gatgccattt
     1501 acgataaagt tcggagttct tatttgcttt catgtattct gaaaaaaaac aaaaatgttg
     1561 ggcttattct tcatgcacca tcatttgttt ctgtgtctga aaaaaatcgc cagaattgtg
     1621 atggcaaatt actcccgtaa ctggagtaat tccgagttgg ccagtgcggt tttaatgagt
     1681 gaatcttctt taaaaagaag aatgtacaaa gaggtcggtt ctataagtac atttgtacat
     1741 aaaataaagc ttacagaagc tatcaggaaa ttacgaagga caaatactcc catttctgtc
     1801 atatcatctg agttaggtta cagttcacca tcgtattttt ctaaagtttt ctttaaatat
     1861 ttaaagacat accctcaaaa cataaggaag aaaaatggtc ggtaatgtca ccaattagga
     1921 ggacttgcag agccgctttt ttacgatact gagatcaaac agacataatc tatgcggaga
     1981 tttacagcag ggcatggttc acaaatctga cagcgatgaa ttatcggcac tgcgggcaga
     2041 aaatgctcga atcatcaagc c
//
LOCUS       DAJTYF010000252         2043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTYF010000252 DAJTYF010000000
VERSION     DAJTYF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.258"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..786)
                     /locus_tag="OO590_004198"
     CDS             complement(<1..786)
                     /locus_tag="OO590_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6639307.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSE"
     gene            1134..2006
                     /gene="rluF"
                     /locus_tag="OO590_004199"
     CDS             1134..2006
                     /gene="rluF"
                     /locus_tag="OO590_004199"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6639308.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
BASE COUNT          514 a          525 c          509 g          495 t
ORIGIN      
        1 ttcgctcata ttcgtttcaa ccattgcgcc gtgaatatca aaaaagcgcg agcaaatatc
       61 gccagcaacc tggcgggcat tcaggtcgtc actctcttca ccaccataaa acgcatgcca
      121 gttagcgccg tcgcgaatgg ccggtgagcc aattcccacc agggcgacat ccagattatc
      181 ccagtaggca gagatggttt taaagtgctg agactgcatg atcccattac gaattaatgg
      241 gttatccagc agagccggaa aatccgcgag atgcgattcc cctttcagct tcgctgccgc
      301 gctgtaggtt aatgtgttta catgatagcg gctttcgagt ttaccggacg gtccgccaat
      361 aattggtacg cagattaact gccgcgattg ccccgcctgc ggcaagtttt caaccagtgc
      421 gctcaccgcg cgaccccagg aaaaaccgac aatatcgcca ggctccagca agcgatccag
      481 cagttgcgcg ccgtgtaacc ccatcatcgc cagttgagtc tcttcatcct catcatttcc
      541 cgacaccacc acaacgtctt tcaggccaaa cttttgcttc agttgctgct ccagccagag
      601 attttcgttg tagtcatagt tgatggcgat ggtgaccatt ccctgatcgc ggcctcgttt
      661 aagcaagcgg ctgatggtgg tgcggtaaat acccagttcg cgcgcgattt gcgcctgcgt
      721 catatcctgt tcgtaataga gttgggcaat cttcacaatc aaacggatat cgtcactgtt
      781 ttccattgcc gcctcctggt tctgcacatt tgtgcaaata cgctttctca ccgcttgatc
      841 tgatataacc ccatcatgag ggcctttcaa agcgattttt cactctttgc atggggagaa
      901 atagcgcttt ctcttgcaca tttgtttctc tgcgtagtag tcatcagcac aggtgtgcag
      961 cgattcgtaa tatgttgatt atccttattt tgccaggcaa atttgcatcg tacttccgga
     1021 gacacttttg tgataaaagg cgtggcagat cacatttaat ttcgacgttg ccgcataacc
     1081 ctgtttttcc ccgctaagag gtacggtata ttccgcgttt tacaggagaa tttatgctgc
     1141 ccgactcatc agtccgttta aataaataca tcagcgaaag cggaatttgc tcacgccgcg
     1201 aagcggatcg ctatatcgag caaggcaatg tgttccttaa tggcaagcga gccaccattg
     1261 gcgatcaggt gaaacccggc gacattgtga aagtaaacgg tcagttgatt gagcctcggg
     1321 aagccgaaga tttggtactt atcgccctga acaagcccgt cggtattgtc agcaccaccg
     1381 aagatggcga gcgcgataac attgtcgatt tcgttaacca cagcaaacgc gtgttcccga
     1441 ttggccgcct ggataaagac tcccaggggc tgattttcct caccaatcac ggcgatctgg
     1501 tgaataagat cctccgtgcg ggtaacgatc atgagaaaga gtatctggtg acggtcgata
     1561 aaccgattac cgatgaattt attcgtggta tgggggcggg ggtgccgatc ctcgggacgg
     1621 tgacgaaaaa gtgcaaagtc aaaaaagaag cgccgtttgt cttccgcatt accctagtgc
     1681 aggggctgaa ccgtcagatc cggcgcatgt gcgagcattt cggctatgaa gtgaaaaagc
     1741 tggaacgtac gcgcattatg aacgtcagct taagcggcat tccgctgggg gagtggcgcg
     1801 atttaaccga cgatgagtta atcgacctct ttaagctcat cgaaaattcc tcttccgagg
     1861 caaaacctaa agcgaaggcc aaaccgaaaa cagcgggcat caaacgtcca gtcgttaaga
     1921 tggaaaaaac ggcggaaaaa ggcggtcgcc cggcgtccaa cggtaagcgt tttacctcgc
     1981 cggggcgtaa aaagaaggga cgctgattaa cgcctcagac tgatgaaaac gtccaaaaag
     2041 agc
//
LOCUS       DAJTYF010000253         2033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTYF010000253 DAJTYF010000000
VERSION     DAJTYF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.259"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(210..374)
                     /locus_tag="OO590_004200"
     CDS             complement(210..374)
                     /locus_tag="OO590_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639309.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1276..1560
                     /locus_tag="OO590_004201"
     CDS             1276..1560
                     /locus_tag="OO590_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6639310.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1548..2033
                     /locus_tag="OO590_004202"
     CDS             1548..2033
                     /locus_tag="OO590_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6639311.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          522 a          426 c          584 g          501 t
ORIGIN      
        1 ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga gccggttact
       61 ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt ctgcaatacc
      121 ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata gctgatggag
      181 ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc gtttccggcg
      241 taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt atgagagatg
      301 aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt aagagcggtg
      361 gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt gcggagatga
      421 acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc gaaaatgtgc
      481 tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc ggcatccggc
      541 atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc tgtcgtctcg
      601 atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg atgcgagaca
      661 caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg agcgaagggg
      721 gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc catgagtcag
      781 ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg ggtcaaagct
      841 aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt taccaatcga
      901 agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt gcccgcaaac
      961 acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga attcgtcacg
     1021 actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg gttcaacgac
     1081 aagagccgta tgacgcgaga gtgtcacgta cggttccgtg ggagcgtagg ggggcggtgc
     1141 ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta aatgccgctg
     1201 acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg tcaatatgct
     1261 attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca gctcaatctc
     1321 agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct tcataagtcg
     1381 cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat tctcgaccgg
     1441 cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct tgatgctccg
     1501 gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg ggatgtgtaa
     1561 ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc ggtgaagctg
     1621 tacttgatga ctggttaaag cagaaggggc taaaaaatca ggcaatcgga gccacccgga
     1681 cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca ctggccactg
     1741 gtagtatcaa tcatacagaa gctacaggta accttcggcg caatatgcca gaccccattc
     1801 ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa gggcttggcg
     1861 cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat atcggtgtgc
     1921 gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt catcatggtt
     1981 ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa tag
//
LOCUS       DAJTYF010000254         2010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTYF010000254 DAJTYF010000000
VERSION     DAJTYF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.260"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="OO590_004203"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="OO590_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6639312.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWGLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYLADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..>2010
                     /gene="mcbR"
                     /locus_tag="OO590_004204"
     CDS             1351..>2010
                     /gene="mcbR"
                     /locus_tag="OO590_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6639313.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YN"
BASE COUNT          547 a          422 c          548 g          493 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gggctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga caggaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtgatgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatta
      721 cctcgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg cgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat
//
LOCUS       DAJTYF010000255         1922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTYF010000255 DAJTYF010000000
VERSION     DAJTYF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.261"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(133..1587)
                     /gene="ospC2"
                     /locus_tag="OO590_004205"
     CDS             complement(133..1587)
                     /gene="ospC2"
                     /locus_tag="OO590_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6639314.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          609 a          331 c          300 g          682 t
ORIGIN      
        1 tccaggattc ccggggcgtt caccacatgc aatgtgttgt tgcaggggaa cggtctgcac
       61 catcccctat atcgatcaat ataacaaaaa tgacaacgtc agattcaggg cacaacctaa
      121 atatatttta ggtcacctcc caaatcgttt gcctgatacc gctccatttt ttaataaaaa
      181 atcaattgtt tttgaatctt tgcttttcat tgcattgtca agcatagtgt ccccactatt
      241 tgctttttga aatcttttgt ttacatcaac caatccatta ttaataaaat attctagcac
      301 tttatagttg gcactatctt cactaagtaa atactcaaca tcatacagct cacaatacat
      361 attgttgttc attttctcca tctctgcgac atcttgtttt gtaaaattaa atttagatag
      421 taaataaagt gccatatcag attttgcatt gcctagagaa taagcaagag cttgatgagc
      481 cattttttta tcagtcacct tattgttaat ttcttcataa ttggatgctt taatggcatc
      541 ctccaaagat atatctctga gtcttatctt tttgaagtta tctgtgctta gcattcttgg
      601 tatatgatac tcaagctgaa acacaaagtt tataattcta tccagactaa caggatcaat
      661 gtttttatta taacaaaact ctcggaatct ttgatcttta ctctttctta taaaatcaat
      721 aagatgcaac ccaattccaa gcctcatatc ttttgtatta aatattggca catcagtttt
      781 tccctgatta ccatgcacat acctgctaac ttcatccaca acctctttaa agttatctaa
      841 gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata gcattatcaa agtggtcggt
      901 taatgtcata tatccatatg gagagtcatg atcaatgata tacgcattag caccaaaatc
      961 cacagtatga tgtgttttat ttagtgggag tgtttcttga tgattggaaa tttcaacccc
     1021 aaaaaaaaca aaatcgtggt tagctatttc ttcaatatcc tcagagaatg tgtttttata
     1081 tgtttgtata ttctcagact taattttatc aaaggactta atattaagag tattatttga
     1141 caggttagcg ttactttgat gtctgaagtt aaaatcagaa ttaataataa tagagagcaa
     1201 gtgacgttca tccgaagaaa gaggatgagt tttttgtatt atttcagaaa gatagtgtct
     1261 ggacacctta tcgaggtgct caagtgaccg aaatgatgac cgtgctccag atggcgcttc
     1321 aatagaaaga tttaatgatt tgaaactggt cccttgagag aacattttac tataactttg
     1381 cgctgctata gacttcctta aaaagctttt cacagtatca gcacagtgat ctaaatgttt
     1441 ttcacttatg ccagaagagg atgagtttgt tacgcatgat atgttttttt gtaatgcttt
     1501 tgtatctttg ttcggattta tttttccatc aacaagatca atattgttct gaacattaat
     1561 atgatttact gcttcaggta ttttcataaa acatccccca ttatgtgcgt ttttatctgc
     1621 agttatttag aataatatta tctaacttaa tattttcttg tatgtattat aacgaacata
     1681 ttttaatatg ttgtacgatt ttcactctga aatcccctga gtttaatcac acttccttta
     1741 ctgaaaggaa ttattgcaac agtattattt gtttttggat atatatcttg cgccttgtta
     1801 tgtagttaat gctcagaata tgatagataa aatagagacg caaaacaggg catgctcata
     1861 acatgatgat aacataaaaa attagtaaca ataaatgaaa gaatcatggg cgctcaacaa
     1921 aa
//
LOCUS       DAJTYF010000256         1905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTYF010000256 DAJTYF010000000
VERSION     DAJTYF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.262"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..781
                     /gene="mppA"
                     /locus_tag="OO590_004206"
     CDS             <1..781
                     /gene="mppA"
                     /locus_tag="OO590_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6639315.1"
                     /translation="DIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLM
                     TEKVLGTGEKPAWHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLK
                     LTLLYNTSENHQKIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVG
                     DYNEPSTFLTLLTSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQ
                     APIAPIYQYTNGRLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(832..1863)
                     /gene="ynaI"
                     /locus_tag="OO590_004207"
     CDS             complement(832..1863)
                     /gene="ynaI"
                     /locus_tag="OO590_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6639316.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT          574 a          424 c          427 g          480 t
ORIGIN      
        1 ggatattccg gggcaggttt atacgccacc gcagctcggg acctattatt atgcctttaa
       61 cacgcaaaaa gggccgacgg cagatcagcg cgttcgcctg gcattaagta tgacgataga
      121 tcgccgcctg atgaccgaaa aagtgttggg gacgggcgaa aagccagcgt ggcattttat
      181 accagatgtt accgccggat ttacgccgga accttcgccg tttgaacaaa tgagtcagga
      241 agaactgaat gcgcaggcaa aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc
      301 gctgaagctg acgcttttgt ataacacttc agaaaaccat caaaaaattg cgattgctgt
      361 agcatcgatg tggaaaaaga accttggcgt agatgttaaa ttgcaaaatc aggaatggaa
      421 aacctatatc gatagccgta acaccggcaa ttttgatgtt atccgcgcct catgggtggg
      481 ggattataat gaaccctcca ctttcctgac attattaacg tcaatgcatt caggaaatat
      541 ttcacgcttt aacaatccgg catacgacaa agttctggcc caggcatcga cggaaaatac
      601 cgttaaagcg cgtaatgccg attacaacgc ggcagaaaaa atcctcatgg agcaagcacc
      661 gattgcacca atttatcaat ataccaatgg acgattaatc aaaccgtggc tgaaaggtta
      721 tcccattaat aaccctgaag atgtagcgta cagtcggact atgtatattg tgaagcattg
      781 atgtgatggg aactggcgtt acccttgtgg ataacgccag tgatgtctga tttagcgacc
      841 ctgttccggc ggtgtaatgt tatccatata cagcgtctgg ctggggaagg caaagtccgc
      901 gccgtgtgac tgtacaatat cgataatctt caaataaacg tcttgctgtg cagcaagcca
      961 ctcagcccat accgtggttt tggtaaagca ataaaccata atattcaatg acgagtcagc
     1021 aaactggttg aaataaacca gtaaggtttg tcgctggtcg atggccgggt gatttttcag
     1081 catctcacgt acggcttcga caataacgcc cacttttgcc gcatcctcat aacgtaaacc
     1141 aatggtcgtg gtaatgcggc ggttggtcat tcgtcctggg ttttctacgc tgatcgacga
     1201 aaacagcgag ttcggtacgt acaatggacg attatcaaag gtcgtaattt tggtaattcg
     1261 ccagccaatt tccgctactg taccttcgat atttctgtcc ggtgaacgga tccagtcgcc
     1321 aatactgaaa ggacggtcga aatagagcat gatcccggaa aagaaattac tcagaatatc
     1381 tttaccggcc ataccgacag ccagaccacc aataccacca aaggtcagca agccagaaag
     1441 gctcatgccg aaatgttcgc cataaagcag aacaagcact acaataatgg tgattttgat
     1501 aatgcgcgac ataatccgcg cactggtgat atcgcgacct tttttaatct gctgtttttc
     1561 aaactgatta atcagcagaa atagcttaat cgtcagaata accgcaatca gggacgtaca
     1621 gataaaatcg ataacgcctg gggtgataaa tttgagttta tagttttcta taacataatt
     1681 aatgatgcta ccaacagcac tgataattat ggtgtagatt aaaaattgca ccgcatggaa
     1741 taaaaatcct tttcttttac gatttccacg gcgaaaccaa aagctcatca gaatcaatgc
     1801 tgcgcagcta ccgaaaataa tgaccagatt aagcgcatta tttgtaaaca gttcagcgat
     1861 cattgtttta tcaggctcct ccagataatt gtcgtcatgc cggaa
//
LOCUS       DAJTYF010000257         1859 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTYF010000257 DAJTYF010000000
VERSION     DAJTYF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.263"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..559
                     /locus_tag="OO590_004208"
     CDS             <1..559
                     /locus_tag="OO590_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6639317.1"
                     /translation="LGLARSSYFYHRAALKAGDKYATIRKMLTDITEFQLPAGKVWLS
                     PVVDCFDGKVVSWSLSTRPDAELVNTMLDSAVETLNAGERPVIHSDRGGHYRWPGWLE
                     RVNAAGLIRSMSRKGCSPDNAACEGFFGRLKTEMYYGRKWSGITPEKFMQQVDAYIRW
                     YNERRIKLSLGAVSPKMYRQQCGLE"
     gene            674..1015
                     /locus_tag="OO590_004209"
                     /pseudo
     CDS             674..1015
                     /locus_tag="OO590_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            998..1835
                     /locus_tag="OO590_004210"
                     /pseudo
     CDS             998..1835
                     /locus_tag="OO590_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          455 a          454 c          571 g          379 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatcacggag ttccagctcc ctgcaggtaa
      121 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      181 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      241 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      301 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      361 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      421 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      481 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      541 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      601 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      661 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      721 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
      781 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
      841 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
      901 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
      961 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1021 ggccgaacgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1081 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1141 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1201 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1261 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1321 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1381 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1441 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1501 aatgcagacc cgggataacc ttgagcgcat ggtggttatc caggcgttta tagcggtacg
     1561 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1621 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1681 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1741 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     1801 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc ttagggggg
//
LOCUS       DAJTYF010000258         1855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTYF010000258 DAJTYF010000000
VERSION     DAJTYF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.264"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..119)
                     /locus_tag="OO590_004211"
     CDS             complement(<1..119)
                     /locus_tag="OO590_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6639318.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSV"
     gene            complement(430..534)
                     /locus_tag="OO590_004212"
     CDS             complement(430..534)
                     /locus_tag="OO590_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6639319.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            complement(809..1486)
                     /gene="kdpE"
                     /locus_tag="OO590_004213"
     CDS             complement(809..1486)
                     /gene="kdpE"
                     /locus_tag="OO590_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR6639320.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(1483..>1855)
                     /locus_tag="OO590_004214"
     CDS             complement(1483..>1855)
                     /locus_tag="OO590_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="sensory histidine kinase in two-component
                     regulatory system with KdpE; signal sensing protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HCR6639321.1"
                     /translation="HVDGPLFERVLINLLENAVKYAGAQAEIGIDAHVEGENLQLDVW
                     DNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIVDVHGGTITAFNRPEGGA
                     CFRVTLPQQTAPELEDFHEDM"
BASE COUNT          510 a          458 c          427 g          460 t
ORIGIN      
        1 actgataaaa taatggcggc aacgtcaata taattacttt cgtcaatatt cacgcatgca
       61 cgatgagtgg aaaaacaatc cgggcaagaa cggctaacgg caatttttaa ttctgacatt
      121 ttattcatct ctttatttca ggtaatagca ggtcctcaat ttctcaaagg agaaatcgat
      181 cacgccggaa aaaaagcaaa gctccaccgc tgataaataa atcagtagtt tgctttttgc
      241 agatgcaata acccagacca gcccggtaac tggctatact cgtcatacct caagttgcaa
      301 gtgctgcgac tcgaattatt tagagtttaa aagggttagc agtatatgag caccgaggaa
      361 tacaggtatt aacctgtaat gcttaaagtc agggagacct gtacgttggg gtgcccttta
      421 gtcagaagac tagcgggcat taaagaaatg aaagtcaaaa ctattacgat gggcaaactt
      481 catgatatgg gttgtccgcc ttatatgagg cattaagcgg ttattatttt ccatttacct
      541 ttaccgcttg ctgtgtcagt tgaaggtaga gttattctat ccatgaaatg gctataatct
      601 gtgaagaaga tcaacaaaaa acgatcatac cccagataat aaatcattaa ttatgaatat
      661 cgccatatta gatgctgttt tgttacttaa atgttgtaat ttcgtgtttt atatttgcgt
      721 taagaaatta aacctgccaa ataataatat tcggcgcagg tttaataata aattaatcac
      781 tatttaagcg aatttattga ataacaattc aaagcataaa ccgataccca ataccggttt
      841 cagtaatgaa gtggcgaggc cgggccggat cctgttccag tttttgtcgc agatgtccca
      901 tataaatacg caaatagtga ctgtgttcga ccgcgtttgg cccccacacc tggttaagga
      961 gctggcgctg ggtgagtact tttccggcat tgttgagcag caccgctagc aggcggaact
     1021 caattggtgt gagatgcacc tcttcctcac cccggtgaat cacgcgggcg gctaaatcga
     1081 cggtaacatc ggaaaatttc accagcggat cgggcgcggc ggtggcggag tggcggcgta
     1141 aagcgacgcg cagacgggcc tgcaattcgc caatgccaaa cggcttactc agataatcat
     1201 ccgctccggc atccagcgcg gcgatcttgt cgctctcttc gctgcgtgcg gaaagcacaa
     1261 tcaccggcac cgggctccac tggcgcaggt cgcggataaa ctcaatccca tcaccatcgg
     1321 gcaggccgag atcgagaata atcaaatctg gcttacgggt tgccgcttcc agcaagccgc
     1381 gttgcagagt ttcggcctca aagacgcgca tcccgtcgcc ctccagcgcc gtgcgcagaa
     1441 agctacgaat agcctgttca tcttcaacaa tcagaacgtt tgtcacatat cctcatgaaa
     1501 gtcttcaagt tcaggagcgg tttgctgggg aagtgtaaca cgaaaacagg caccaccttc
     1561 cggtcggttg aacgcggtaa tggtgccgcc gtgtacatcc acaatcgccc gacaaattgc
     1621 caacccaagc ccgacacccg gcaccgccga ctctttattc ccgcgagcaa acttatcaaa
     1681 tatcgtctgc tcctggcctg gcggaagacc ggggccgtta tcccagacat ccagttgtag
     1741 attttcgccc tcaacgtggg catcgatacc aatttcggcc tgcgcacccg catatttcac
     1801 cgcgttctcc agcagattaa tcagcacccg ttcaaagagt ggcccgtcaa cgtgg
//
LOCUS       DAJTYF010000259         1836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTYF010000259 DAJTYF010000000
VERSION     DAJTYF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.265"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..356)
                     /locus_tag="OO590_004215"
     CDS             complement(<1..356)
                     /locus_tag="OO590_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6639322.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVCGGCP"
     gene            complement(356..1276)
                     /locus_tag="OO590_004216"
     CDS             complement(356..1276)
                     /locus_tag="OO590_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6639323.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1414..>1836
                     /gene="pgrR"
                     /locus_tag="OO590_004217"
     CDS             1414..>1836
                     /gene="pgrR"
                     /locus_tag="OO590_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6639324.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERF"
BASE COUNT          458 a          452 c          422 g          504 t
ORIGIN      
        1 gggcaccccc cacacacacc cagcacgcca atgcgttcag catcaacata tggcagagtt
       61 actgcataat cgataacacg gctaatatcc tctacgcgct gtgtcggatc ttctatccaa
      121 cgaggctccc caccagattc tccctggaaa ctggcgtcat aagcgagaac gacatagcct
      181 ttttcagcca atgccttgcc atagatattg ccagacgtct gctccttaca actgccaaaa
      241 ggatggacgc tgataatcgc cggatactgt tttgactcat caaaatttgg tggaaactga
      301 atattggcag caatatgcca ggccatatca ctattgcgga tttgaatatg tttcattaca
      361 gagtttcctc aaagaacgga gctaatactg aaattgcttc agccacatag gctttgccgt
      421 cataaagatc catatggttt gcgccttcaa caatgtgata gcgtttatcc tggcttgatg
      481 cccgatcgta caggtcgtca ctcatccatt tgctccctgc ctggctgccc gccacaatct
      541 gcatcggctg agtcaggtac acttccgcca tatggtaagc atcataggta ataatctggt
      601 taaggctgcg caaagtagcg taacccggtg ctgttggata ctgcgcacga ggtgtgtggt
      661 aatactccca ggcctgacgc agttcttcgt tcggcgcatc ggcctccttc attggagcca
      721 gtggcataat ggcgtattct tcgctgctaa tatcgctggt tcgggcgttt gagccagctt
      781 caatgtgtgg cagagcatca atagatttca cattgttttc ccaaccattg cggaacatcg
      841 aaccaatatt gaccgcacta acggtaccga tggccttgat gcggcgatcc tgaattgcag
      901 cattggctgt atatcctgca ccggcacaaa ttcccatcgc accaattcgg gtattgtcga
      961 cataagaaag cgttgtcagg taatcaatca cggcactgac gtcttcagta cgaatgtatg
     1021 ggttttctaa ctgacgcggc tcgccgccac tttcaccttg ataagatgcg tcataagcaa
     1081 tagtgacaaa tcccttttcc gccagctttt cggcataggt tccggccgtt tgctctttaa
     1141 cgcccccacc tgggtgagat accacaattg ccggatactg acgggtttca tcaaattttg
     1201 gagggaaata gatcactgca gacaaagaga tggttggatt attgctgtta gtgaagctga
     1261 ctttattatt catcattcag ttcctcatgg agttgtcggt tcgttttgac ggttggtgat
     1321 atcactatag atattgatca ttaagttgat tagaccctaa atcatgatta gacctatcat
     1381 ttaaatgatt aatagattgt atggagatac tgaatgaagc gtgaagaaat cgctgatctg
     1441 atggcgtttg tcgtcgttgc agaggagcgt agcttcactc gcgcagcagc ccgcctgagc
     1501 atggtgcagt cagctttaag ccagatagtg cgccgtatag aagaacgatt gggattgcgg
     1561 cttctgacgc gaaccacgcg cagcgttgta cccactgaag cgggcgagca tcttttgtct
     1621 gttcttggcc ctatattgca tgacatagat tctgccctga catccctgag cgatctgcag
     1681 aaccgcccat ccgggacaat acgtattact actgtagaac atgcagcaaa aacgatattg
     1741 ttaccagcca tgcgcacatt cctgaaatcg catcctgaaa ttgatattca gcttaccatt
     1801 gattatggtt tgaccgatgt cgtttctgaa cgtttt
//
LOCUS       DAJTYF010000260         1829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTYF010000260 DAJTYF010000000
VERSION     DAJTYF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.266"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            220..1728
                     /gene="ltrA"
                     /locus_tag="OO590_004218"
     CDS             220..1728
                     /gene="ltrA"
                     /locus_tag="OO590_004218"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6639325.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          462 a          455 c          532 g          380 t
ORIGIN      
        1 ggcagcctcc tcagtatgcc tgagtccagg caggtaaacc ggggaaggtc agcgacggat
       61 gttaaggggg catggctccg atgacgcgct ggctggcgga gcttccgtag tagtccgcga
      121 tggggaaagc ccattacatg gcgaagggaa gcagtttgaa tgtgtttgcg acgtgaatta
      181 actgacctaa cgaggtgaag acctttgata atcagcgaaa tgcaacgcaa gcttgccaca
      241 tgggcagtca ccgatccgtc cctacggatt caacggctgc tgcgtctgat aacacaacca
      301 gaatggctgg ctgaagcggc gcggatcacg ctttcatcaa agggggccca tacccccggc
      361 gttgatggcg tgaacaaaac aatgctacag gccagactgg ctgttgagct gcaaatcctc
      421 agggatgaat tactctcagg ccactaccag cccttgcccg ccagacgggt ttacatccct
      481 aaaagcaacg gcaaactgcg accactgggt atccccgcgt tgcgggatcg tattgttcag
      541 cgggccatgc tgatggcgat ggagccgata tgggagagtg attttcatac gctctcatat
      601 ggcttccggc ctgagcgcag tgtccaccac gctatccgca cggtgaaatt acagctcaca
      661 gactgtggtg aaacccgggg acgctgggtg attgaaggcg acctgtccag ttacttcgac
      721 accgtacatc atcgactgct gatgaaggct gtacgccgca ggatcagtga cgcacgtttc
      781 atgactctgc tgtggaaaac catcaaggcg ggacatatcg atgtcggtct ctttcgggcg
      841 gccagtgaag gtgtgccaca gggcggtgtt atatcgccgc tattatcgaa catcatgctg
      901 aatgagttcg atcaatacct gcatgagcgc tacctgagcg ggaaagccag aaaagatcgg
      961 tggtactgga ataacagtat ccaacggggc cgaagtacgg cggtcagaga aaactggcag
     1021 tggaaacccg cggtggcgta ctgccgctat gccgatgatt ttgtcctcat cgtcaaaggc
     1081 accaaagcac aggcggaagc catcagggag gagtgtcggg gtgtgctcga aggcagtctg
     1141 aaactcaggc tgaacatgga taagactaaa atcacccatg ttaatgacgg ctttatcttt
     1201 ctggggcaca ggatcattcg caaacgcagt cgttatggcg agatgcgagt ggtctcaacg
     1261 atcccgcagg agaaagccag aaacttcgcc gcatcgctga cagcactgtt atcaggcaac
     1321 tacagtgaaa gcaaagtcga tatggctgaa caactcaacc gaaaactgaa aggctgggcc
     1381 atgttctatc agttcgttga ttttaaggcc aaagtcttca gttatatcga ccgtgtcgtg
     1441 ttctggaagc tggctcactg gctggcccgc aaataccgta caggtatcgc ttccctgatg
     1501 aggtggtggt gtaaatcacc gaaaccgggt cagagcaaaa cgtgggtttt atttggtaaa
     1561 accaatcacg gcaagctcag cggcgaaata ctgtaccggt tggtggggca aggcaagaag
     1621 ctgttccgct ggcggctacc cgaaggtaat ccctatctga ggacggagac cagaaacacg
     1681 tatacatcgc gctttacaga agtggcaatg gcgttcgcca gcatttaaat ggagagccgg
     1741 atgcgctgaa aggtgcacgt ccggttcggg gaggagaggc agggaaatag tccgactacg
     1801 ccctgcctct tactctactc agcacatgg
//
LOCUS       DAJTYF010000261         1819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTYF010000261 DAJTYF010000000
VERSION     DAJTYF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.267"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..454
                     /locus_tag="OO590_004219"
                     /pseudo
     CDS             1..454
                     /locus_tag="OO590_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            514..621
                     /locus_tag="OO590_004220"
     CDS             514..621
                     /locus_tag="OO590_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6639326.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="OO590_004221"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="OO590_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6639327.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..>1819)
                     /gene="yehX"
                     /locus_tag="OO590_004222"
     CDS             complement(1338..>1819)
                     /gene="yehX"
                     /locus_tag="OO590_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6639328.1"
                     /translation="AADPQVLLMDEPFGALDPVTRGALQQEMTRIHRLLGRTIVLVTH
                     DIDEALRLAEHLVLMDHGEVVQQGNPLTMLTRPANDFVRQFFGRSELGVHLLSLRSVA
                     DYVRREERAEGEALAEEMTLRDALSLFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
BASE COUNT          445 a          556 c          452 g          366 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgca cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attagccgga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagcca
//
LOCUS       DAJTYF010000262         1803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTYF010000262 DAJTYF010000000
VERSION     DAJTYF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.268"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..285)
                     /locus_tag="OO590_004223"
     CDS             complement(<1..285)
                     /locus_tag="OO590_004223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639329.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIP"
     gene            complement(1038..1511)
                     /locus_tag="OO590_004224"
                     /pseudo
     CDS             complement(1038..1511)
                     /locus_tag="OO590_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1439..1803)
                     /locus_tag="OO590_004225"
                     /pseudo
     CDS             complement(1439..1803)
                     /locus_tag="OO590_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          416 a          503 c          421 g          463 t
ORIGIN      
        1 aggaattgtc gcaaattccc gctccgtgca gcgggcaatt tccggatacc cttcgtcatc
       61 aacacgtaca aaccagaaga ccagtttttt gtttccggca tccacaaaga acggaatatt
      121 caggtctgcg caacactcaa cggcatcgtc aaaattatca aagcgcagaa cctcagcttc
      181 ttcttcgtca aaaaaatcat cttcgtgaag ttttacgaca tagcggagga gtttgcttct
      241 ttgaggtgca ggttttcgtt tgcgtggttt agctgaacgg gccatataac tacctgaaag
      301 acaatgatat tgtctgtttt tataacggta atcacaggcc atgacaagcc gcagccgtca
      361 ggccgcctac tcggggcggt ttagaacgct tatttagtac cagatcttac ttataataaa
      421 ggggcttggc agtttggata aactattgtc cttattccac taatacaaac tttttgtaaa
      481 gtatattaaa tatacttcat ttcatactga aatccccgga agtattcaat caaacaatta
      541 caccataaaa ggaagagggg gccccaatta agttgtcaag catgtttact gcgcaatcgc
      601 ttggtgctcc gtccctgtac cccgcattgg tactctggcg ctcatggggc ttccacttgc
      661 gcttctccct tcacatcggg gtgacaggtc cacgttcccc acaagagcct gaatcgactt
      721 cacgccgctt ttatgccgga tgccgcgcag gcagtaaaca tatcccacag gaacgactcc
      781 accattgttt tgacatcatc tatatgcttt cgacacatca tcagcggttc actttcgttc
      841 gtctcttcga accatacctg atcggctcac gtccgacctt ttcccatctc gctcaccacc
      901 acatctctta aatgcagcag cagtggatgg tttggtgcct gcgcctgcac gccgacaccg
      961 aggggccttc cctcatctct tgtgctgcat ggctgcacag ggactcacgt ccccaggacg
     1021 cgccttcgtg gcacacagtc atcggcatag caacagtaag caacggcagg tttccattgc
     1081 cagttttctt tgacagcagt acttcggcct cgctggatac tgtgattcca gtaccagcga
     1141 tcctttctgg cttttccact caggtagcgt ttattcagat actggtcgaa ctcattcagt
     1201 atgatgttcg acagcaacgg cgagatcaca ccgccctgtg gcatactttc actgaccgcc
     1261 cggaagagac caacatcaac acggcgtttt ccacagcagt ctcataaaac gtgcatcact
     1321 gatcctgcgg cagacggctt tcatcagcag ccggtgatgt actgtgtcga agtagctgga
     1381 caggtcacct tcaataaccc ggcgccccgt gtttccccgc agtcggtgag ctgtaatagt
     1441 acgtgatgtc tcccgcccac ttctggttcg ggccactggc gtaaaaatcc tgcgtcagca
     1501 gattttctga tacaggcagg ccgtgtgcac ggtaactgat cgggctgaac ttctgggagg
     1561 cttttgcgct cagttcctga cggtgcagac tggctgccac ggtttttacg ttaaagtggt
     1621 aaccagagca cacagttcat ccgtcaggcg tggggcaccg taacgctgga ctgggtaaaa
     1681 gccttgcgga caacgctgtc gcagtgctga cggaactgct gacgtgtgtt tatccttgtc
     1741 cgccgctgac accacgtata ccagccgctg cgggccaccc ggagtacgcg gcacattgcc
     1801 ttg
//
LOCUS       DAJTYF010000263         1796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTYF010000263 DAJTYF010000000
VERSION     DAJTYF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.269"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(178..579)
                     /locus_tag="OO590_004226"
                     /pseudo
     CDS             complement(178..579)
                     /locus_tag="OO590_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1008..1796
                     /gene="virF"
                     /locus_tag="OO590_004227"
     CDS             1008..1796
                     /gene="virF"
                     /locus_tag="OO590_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6639330.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          561 a          329 c          331 g          575 t
ORIGIN      
        1 cttccttatg ttatatttta gtaaagaatt tattttttac attatagtcg gctaaatgaa
       61 gtttactgta aaaaaacgat acttttttcg taagcgatga gatgtggagc cagaaaaatg
      121 gctgcgttac gtcattgaac tgtccttgac ccacgatatt cagacagtcg ttgtatctta
      181 agttgacatt acccgctgac gtcgatattc ccttggtgag agataaccca acgcactatg
      241 cggatggttt tcattgtaat gctcgatcgc cactgcaagg ttatgcaatg ccgttcttgg
      301 tttcggcatg aacgcgatgc agtcttcctt catcgttttc acgaaccgtt ctgctattcc
      361 attactctga gggctactga ctgccgtcgt gcagggctcc agattcaact ctctggcgaa
      421 ctgccgtgtt tcatgagccg tataagccga accgttatcg gccactggat tggccgttcc
      481 ggtcctttat cgccatatca ctcctgacat gacatcctgc accgttgctt tgtcataatc
      541 tcccgtgcct gctgcccagt ctatggcttc acgatcgcaa tatggaacgt aacgctgtca
      601 tcgggagaat cgtcaatgcg gcattcggtt aacgcttacc aatatcaaca ccaaccggat
      661 ttgtggagcc tccagtctga aggggcttta tgcgttccgt ataggatatt atgatgctgg
      721 agttttgcga agctccactc ctttccgaac atttacacag atattgccta agaaaagtga
      781 aaccataata tggttatagt ccctttcagt gcaaatactt agcttgttgc acagagaaat
      841 agaagctgca taagctcttt cttcaaaaaa tgtaaataaa gttaaatata ggaaaaatta
      901 cttaatctat cttaataacg gaaagatttt gtatacaatc actgttacag aaatttctta
      961 gttactctgt aaacactaaa tatagtttgg tatattctgt tgaatttatg atggatatgg
     1021 gacataaaaa caaaatagat ataaaggttc gcttgcataa ctatattatt ttatatgcaa
     1081 aaaggtgttc aatgacggtt agctcaggca atgaaacttt gactatcgat gaagggcaaa
     1141 ttgcttttat agagcgaaat atacaaataa acgtctccat aaaaaaatct gatagcatta
     1201 atccatttga gattataagc cttgacagaa atttattatt aagcattatt agaataatgg
     1261 aaccaattta ttcatttcaa cactcctatt ctgaggagaa aagggggtta aacaaaaaaa
     1321 tattcctcct ctctgaggag gaggtttcta tcgatttgtt caaatctata aaagagatgc
     1381 ctttcggcaa aagaaagatc tatagtttag cttgcctttt atcagctgtt tctgatgagg
     1441 aagctttata tacttcgata tcgatagctt cttctcttag tttttctgat cagataagga
     1501 agattgttga aaaaaacatc gagaagagat ggcgtctttc tgatatttca aataacttga
     1561 atttatcaga aatagctgtt agaaaacgat tggagagtga aaaattaaca tttcaacaaa
     1621 tccttcttga tattcgcatg catcatgcag caaagctttt attgaatagt caaagctata
     1681 ttaatgatgt atcaagactt atcggaatat caagcccatc ttattttata aggaaattta
     1741 atgaatatta tggtataact ccaaagaaat tttacttata tcataaaaaa ttttaa
//
LOCUS       DAJTYF010000264         1765 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTYF010000264 DAJTYF010000000
VERSION     DAJTYF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.270"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..122
                     /gene="kil"
                     /locus_tag="OO590_004228"
     CDS             <1..122
                     /gene="kil"
                     /locus_tag="OO590_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6639331.1"
                     /translation="NIKKRKLYIRSLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            122..292
                     /locus_tag="OO590_004229"
     CDS             122..292
                     /locus_tag="OO590_004229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639332.1"
                     /translation="MTKKIKCAYHLYNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            367..642
                     /locus_tag="OO590_004230"
     CDS             367..642
                     /locus_tag="OO590_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639333.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            742..>1765
                     /locus_tag="OO590_004231"
     CDS             742..>1765
                     /locus_tag="OO590_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR6639334.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKE"
BASE COUNT          550 a          396 c          399 g          420 t
ORIGIN      
        1 acaatattaa aaagcgaaaa ctttatattc gtagcctgac tacaaaaaca tgcatttctg
       61 actgcatgat taaagtcttc ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt
      121 aatgactaag aaaataaaat gtgcttatca cctttacaat aaagaaattg aagaaagcaa
      181 aatcattaca agaccacttc atttcatgcg tggagttata ccaacgacgg aaatgaaaaa
      241 atattgtagt gaaatctgtg ccgaaaaaga ccagatggca cacgaacttt aattaactga
      301 ctatccgaaa ctgaatttat gccagcaatg gcagggattc gctcaacctt aattaaggag
      361 aaaaacatga ttaccagtta tgaagccact gttgtaacta ctgatgacat tgttcacgag
      421 gttaatctgg aaggaaagcg tattggctac gtgattaaga cagaaaataa agaaacccca
      481 ttcactgtgg ttgatatcga cggtccatca ggcaacgtta aaacacttaa cgatggtgta
      541 aaaaaaatgt gtctggtgca cataggaaag aatctgcccg cagaaaaaaa agccgaattt
      601 ctggcaactc tgattgcaat gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac
      661 actgtgcagg cctgagtgaa gaacctggga catttattca tcactcgcag taattttaat
      721 ctgagttgag gttaaaaaac aatgaacacc gataaacaag tttacccact gtattacgaa
      781 gcaaaaaatg acaaagtaag aaaacgtctc ggtattaaag gcggttttta ctgggctgaa
      841 gcgaaaaaat tatccattgc catctcccgt ggtgctgttg cgattgacga tgcaggctac
      901 gatgaagatg actttaaaaa acctgttcgc gtcaatttgc ccgttgttga tgacctcccg
      961 ccagaaggcg tatttgatac ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc
     1021 atcacaatgg tatttatcgc atcttctccc tctgttcagg acaaaccggc cagcactgac
     1081 aataccaacg tcaacggcga agacatgaca gaaattgagg agaacatgct cctgccagtt
     1141 tccggtcagg aactgcccat tcgctggctt gcgcaacacg gcagcgaaaa accggtaacg
     1201 cacgtttcac gcgacgaact ccaggcatta cacattgctc gggatgaaga actaccagct
     1261 gttactgccc tagctgtttc ccacaaaacc agcctgctcg atccgctgga aattcgcgat
     1321 ctccacaaac tggtgcgtga tactgacaga gttttcccta atccaggcaa ttcaagcctg
     1381 gggctgatga ctgctttttt cgaagcatac atggacgcag actacaccga tcgcggtctg
     1441 ctgacaaaag agtggatgaa aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc
     1501 gctaatgctg gcggcgggaa cctcaccgat cgcggcgaag gtttcgttca cgatctgacg
     1561 tcactggcgc gcgacgtagc cactggcgta ctggctcgtt caatggacgt ggacatttat
     1621 aaccttcatc cggcacacgc taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc
     1681 ttttctgttt tccgcgacaa attcatcacc atgcctggcg ggatggatta ttcccgcgcc
     1741 atcgtggttg cgtcagtgaa agaag
//
LOCUS       DAJTYF010000265         1764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTYF010000265 DAJTYF010000000
VERSION     DAJTYF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.271"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..274
                     /locus_tag="OO590_004232"
     CDS             <1..274
                     /locus_tag="OO590_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639335.1"
                     /translation="YLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYK
                     TDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            276..880
                     /locus_tag="OO590_004233"
                     /pseudo
     CDS             276..880
                     /locus_tag="OO590_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            867..>1764
                     /locus_tag="OO590_004234"
     CDS             867..>1764
                     /locus_tag="OO590_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6639336.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          418 a          404 c          494 g          448 t
ORIGIN      
        1 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
       61 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      121 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      181 cggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      241 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      301 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      361 tttccccgac agttttatca gtgattttta tggtcttatc tggaacatcg ataccgcaga
      421 aaagtatggt cttgatgtct ggggaaagat tgtgggtgtc agtcgccggc tgacggtaaa
      481 ggacgatttt aattacctgg gcttcagcga gtccaggatg gacaccccgg taatggatga
      541 tccctgtccg tttaatcagg caccgtttta caacggcaaa tcggataccc ggactgttga
      601 cctgtctgat gctgtatacc ggcggctgat actgatgaaa gccatgtcga acattactga
      661 ctgttccgtt ccggatatta acctgatgct gagatttatg ttcggaaaaa aacgccgggc
      721 ttatgttctg aataatggcg ggctgaggat gagttacgtc tttgagtccg cgctctcgtt
      781 ggcagaactg gcgattatcc agtcgtcggg tgcactgcca tccccgccgg gtgtttatgt
      841 ttcagtagtt ttaaaggagt cccgtaatga aggccagtga taaaccccgc cagctggcgg
      901 tcccctttgc gagtaccgga gataaaaacc gtatcccgga caaggcgaca cagcagacca
      961 gagagagcgg taatgctgcg tatgattcag gttttcctcc ggtgaccatg acagcggtct
     1021 cagcgggagg tataccgcca cacggcaagg attttaacgg tctgatgtac gatattaccg
     1081 cagcaatacg gttcgcccag gctggcggtt tgtacacgta taatgccggt tttgcggggg
     1141 ccattggtgg atatgcaaaa ggagccattc tcgccggagt cgcaacaaca gcggtctggc
     1201 tgaataccac ggacgataac ctgaccgatc ctgaaggctc cgacagtgcg ggctgggtaa
     1261 atcttcttga ggatccgaaa aggatattcc tgcggcagaa gaacaatctg tcagaccttc
     1321 agaataaagg gacggcacgg gataatcttc aggtttacag taaagagcag tcagatcaac
     1381 gctatgttca tcgggaaggc gataaaataa ccggagagct gaaaatccgt ggtgttaatg
     1441 cgctgaggat tttcaacgaa gcttttggcc tgatttttcg tcgttcggaa gagtgcctgc
     1501 accttattcc caccagtgaa ggtcaggggg aaaatggcga tattggtccc ctgcgcccgt
     1561 tcaccattaa tttgcggacg ggtgaaatat ccatgtcgca taaagtgtct gttggcggtg
     1621 gttcgcaggt caatggtgcg ctgggtatcg gcgttcagaa cgccctgggg gggaattcaa
     1681 ttgttcttgg tgataatgac accggattta aacagaatgg agacggtatt ctggatgttt
     1741 atgctaatag tcagcgtgta ttcc
//
LOCUS       DAJTYF010000266         1751 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTYF010000266 DAJTYF010000000
VERSION     DAJTYF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.272"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..800)
                     /gene="ipaH7.8"
                     /locus_tag="OO590_004235"
     CDS             complement(<1..800)
                     /gene="ipaH7.8"
                     /locus_tag="OO590_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6639337.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGPQIYFSMSDGQ"
     gene            1129..1308
                     /locus_tag="OO590_004236"
                     /pseudo
     CDS             1129..1308
                     /locus_tag="OO590_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1325..1751)
                     /locus_tag="OO590_004237"
                     /pseudo
     CDS             complement(1325..1751)
                     /locus_tag="OO590_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          518 a          310 c          390 g          533 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc tgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggattgcag aacgcgagtc gacaatggat tccctgaaat attaatcctg
      121 taacttgaat ctaattgtga aaaactctcc ggaagagtgg ttatctgatt aaaatgaaaa
      181 taatattcct tttgtctgat attgtttcta tcagaaaatt gaggaaaagt aaccagatta
      241 ttgcgctgag cacagagaat ttccagttta tccggtaagt cgggtaatgt tgtcagcctg
      301 ttataataaa catgtaaaaa tttcaatttt tcgggaagag ccggtaagtt atatagcctg
      361 ttattttcaa caaataatga tgttaaatgg tttggcaaca caggaaggtt ttccagttga
      421 ttagaacgaa cacttaaagt tgttaatgac tcaggaagtt ctggtatcac agaaagcctg
      481 ttattatcag cattaagttc tttgagaaat gcaggcagct ttggcaatga tgttaattta
      541 ttattttcta caatcaaagc agaaatatgc tttggtaatt cgggaagaga cgtcaaattt
      601 aggtctgata aatttaatac tgcttcttga ttctgaaagc actgggagag tctgttaaaa
      661 gcaatgcctc gctcttcccc gggagaagag ttcttttccc attcagtcag gattctcaga
      721 taatgttcat tactggttga gtttgagtta atagaggggg agcaagaaac tgatgagtgt
      781 gtattattta cagagaacat aatgctaggt caaccttata tttgtgagaa taaatattta
      841 aaattgtata tttatacaaa tttcacttac catttccgat attaagtttt aatagtttcc
      901 attttacaca taacagaagt tatgttgtaa tttatttatg gaggttttct atggaattct
      961 tttttattgt gactgcagtc aataataaca ctgaattcaa cattccagtg agcaaggaaa
     1021 tgtaatccga gtaagtatat ttgttttaca tcatatttta gtcaagctca tttattccta
     1081 ttatagaatg ataaattaaa attactgtaa aaaaacgata cacatttaat acgagggagg
     1141 ccggcagaaa aacggcttgc ggtaaggcag gagaaatgtg tgccgcgaat gaagtccttg
     1201 tatgactgga taaagctcca gaggaaaact ttgtcaaacc atgctgaaat ggtaaaggcg
     1261 ttcaactata tcctgaatca ctggaatgca ctgaatgagt tttaccgggt agtacttcca
     1321 atcattagaa tatgtgcttt tcaatgaagg aaccgatgac tttttcgtgg atttccacgg
     1381 aacgcgagaa gcagattgtt ttacgtgcca gccgcttgat gcgggggcgg agggtcaggt
     1441 tgttacgttc atacgttgcg tgaagatttt gccggtcagg tgtttctcct ccggcacttc
     1501 tctggcgtag ctgccccagt catcgctggt caccatgccg atacagaacg gagtcaacag
     1561 ggctaacagt tcccgacagg tctcatcatt ccgtggcccg caagtgtaag ccaacacacc
     1621 gccagtttta gtgttgtacg cataccaggg ccagtgttgt tgcgccttgc tgccaacgaa
     1681 gcgccactgc tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga
     1741 tgttatccgc c
//
LOCUS       DAJTYF010000267         1725 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTYF010000267 DAJTYF010000000
VERSION     DAJTYF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.273"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(120..1334)
                     /locus_tag="OO590_004238"
     CDS             complement(120..1334)
                     /locus_tag="OO590_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR6639338.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          517 a          419 c          356 g          433 t
ORIGIN      
        1 cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc catttttagc
       61 tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc gaaccgttat
      121 taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc cgggtcatac
      181 ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc atcgcctggg
      241 tgcatataac cttcctcgaa gcgccagctg tgcgggaaaa cttcaagcat ttgttcggaa
      301 tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg cagcgccgcc
      361 gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac ctgataaagc
      421 gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt gatcgtcgtg
      481 cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat actgttaaac
      541 acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa acattcctgg
      601 ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat gctcttccca
      661 aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag atgttcgtta
      721 aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag atacttttct
      781 gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata cgccagtcct
      841 ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg cacacgaatc
      901 gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc aatcgtacgc
      961 ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag cagttgatac
     1021 aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat ggcagaaatg
     1081 gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa ttgccagata
     1141 tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc tttcagataa
     1201 gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca aagtccgctt
     1261 tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa aacatctgca
     1321 gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa acctaatttt
     1381 acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac attaaaaaca
     1441 aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt aaccattatt
     1501 gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga aaatgagcac
     1561 agaaatagaa attaattata ttcgattaat taaaatgtgt attgcttgat gtatttaaaa
     1621 atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa ggaatgtgac
     1681 aaataatggt tctggttggg cgttaaattt aaggtcacca gaatg
//
LOCUS       DAJTYF010000268         1709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTYF010000268 DAJTYF010000000
VERSION     DAJTYF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.274"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..345
                     /locus_tag="OO590_004239"
     CDS             <1..345
                     /locus_tag="OO590_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639339.1"
                     /translation="IGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMF
                     TEMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAIYLLAGCLISA
                     MTALLMKDNQRS"
     gene            447..>1709
                     /gene="amn"
                     /locus_tag="OO590_004240"
     CDS             447..>1709
                     /gene="amn"
                     /locus_tag="OO590_004240"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6639340.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVSYG"
BASE COUNT          404 a          429 c          403 g          473 t
ORIGIN      
        1 atcggaacgt tgagcgcatt tcctttcttt atggcgcttg aagcacaatc tattttctgg
       61 atagttttct tctccataat gctggcaaac attgcgcatg acatggtggt gtgtgtgcaa
      121 caaccgatgt ttaccgaaat gtttggtgcc agttatcgct acagtggtgc tggagtcggt
      181 tatcaggttg ccagtgtggt tggcggtgga tttacacctt ttattgccgc tgcactcatc
      241 acttactttg ccgggaactg gcatagcatc gccatttatt tgctggctgg atgcctgatt
      301 tctgcaatga ccgctttatt gatgaaagac aatcaacgct cgtgatagcc tgccgaagat
      361 catccgatct tcgccttata cttttgtttc acattcctgt gacatactat cggatgtgcg
      421 gtaattgtat ggaacaggag acacacatga ataataaggg ctccggtctg accccagctc
      481 aggcactgga taaactcgac gcgctgtatg agcaatctgt agttgcttta cgcaacgcca
      541 ttggcaaata tattacaatt ggcgaattac ctgatgaaaa cgcccgtaaa caaggtcttt
      601 ttgtctatcc atcactgacc gtaacctggg acggtagcac aacaaatccc cccaaaacgc
      661 gcgcatttgg tcgttttact cacgcaggca gctacaccac cacgattact cgccctactc
      721 tctttcgttc gtatctgaat gagcaactaa cgttgcttta tcaggattat ggtgcgcata
      781 tctctgtgca accctcgcaa catgaaatcc cttatcctta tgtcatcgat ggctctgaat
      841 tgacacttga tcgctcaatg agcgctgatt taactcgcta cttcccgaca acagaactgg
      901 cgcaaatcgg cgatgaaact gcagacggca tttatcatcc aacggaattc tccccgctat
      961 cgcattttga tgcgcgccgc gtcgattttt ccctcgcacg gttgcgccat tataccggta
     1021 cgccagtaga acattttcaa ccgttcgtct tgtttaccaa ctacacacgt tatgtggatg
     1081 aattcgttcg ttggggatgc agccagatcc tcgatcctga tagtccctac attgcccttt
     1141 cttgtgctgg cgggaactgg atcactgccg aaaccgaagc gccagaagaa gccatttccg
     1201 accttgcatg gaaaaaacac cagatgccag catggcattt aattaccgct gatggtcagg
     1261 gtattactct ggtgaatatt ggcgtgggac cgtccaatgc taaaaccatc tgcgatcatc
     1321 tggcagtgct acgcccggat gtctggttga tgattggtca ctgtggcgga ttacgtgaaa
     1381 gccaggccat tggagattat gtccttgcac acgcttattt acgcgatgac cacgttcttg
     1441 atgcggttct gccgcccgat atacctattc cgagcattgc tgaagtgcaa cgtgcgcttt
     1501 atgacgctac caagttagtc agcggcaggc ctggtgagga agtcaaacag cggctgcgta
     1561 caggtactgt ggtaaccaca gatgacagga actgggagtt acgttactca gcttctgcac
     1621 ttcgttttaa cttaagccgg gccgtagcaa ttgatatgga aagtgcaacc attgccgcgc
     1681 aaggatatcg tttccgcgtg tcatacggg
//
LOCUS       DAJTYF010000269         1708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTYF010000269 DAJTYF010000000
VERSION     DAJTYF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.275"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..606
                     /locus_tag="OO590_004241"
     CDS             <1..606
                     /locus_tag="OO590_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6639341.1"
                     /translation="AKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAM
                     VFNGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            638..1246
                     /locus_tag="OO590_004242"
     CDS             638..1246
                     /locus_tag="OO590_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639342.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKSVLAVNSEQFRGLTVERLAKYIEMA
                     KHIDHEISDNGYRPSEGYLPAAERRSLMLAFGVSGVSMWPSA"
BASE COUNT          493 a          348 c          432 g          435 t
ORIGIN      
        1 gctaaagcgt actcgcagaa gacctcaggt aagccatacc attattacgt caccgctctg
       61 gctgcgatta caggtgcgag acttaacgag gtggcccaac ttcaggttaa agatgtcagg
      121 accactgagg ctgggaccgt ttttatccat atcaacgaag acgatagcag cctgccgggt
      181 aagagtatca agaacgccca cagtgaccgc tgtgtcccgt tggtagatgg tgcttatggt
      241 tttgtattgg ctgactttat gtcactcgtg gaagaccgta gaaagactga gggtgataat
      301 gctatggtgt tcaatggcct caagctaatg aagaacggct acggtgagca ggttagcaag
      361 tggtttaacc gaacgttgct gcccaaagtc ctcgctgacc gtagcggctt agcttttcac
      421 tcgtttagac atacagttgc gacccagtta aaacaacatg gggtcgagtt agcgtatgct
      481 caggcgatta tgggtcatag ctcagggtct ataacttacg accgttacgc gaaagaggtc
      541 gaggtggaaa cgctgaaaga aaaattagcg gagtcattgt cagtaaaaaa aatagacgga
      601 aagtaatcgc atagggatag cggaatgtga ggacgtaatg gtcaaagaat ttttagttag
      661 caaagaaaat aagccttcat atgaatgtat gaatactgct gaaacttgtt tggggctggc
      721 tggaatggct tcggaagatt ggcaacgtga gcatcattgc cgcacaggta taatatttac
      781 tgcttttgct attgaggcaa tgttcatctt ctatagaaaa caggttgacc ccggctacga
      841 taagacgcag aaagagtgca gaaagacaat gcacaagaat acgttaaaat tgtgcggtat
      901 aaacaattat atggggacaa aaccttacca aattatcaaa gagtgtcttg aggtaagaga
      961 cgctattgct cacggcgatt cgtatacttc gagctttaat ttctcggcgg atcatttgga
     1021 taaccaagac gatattgcaa aaagtgtcct ggcggtaaac tctgaacaat ttcggggatt
     1081 aactgttgag cgccttgcaa aatacattga aatggcaaag catattgacc atgagatttc
     1141 tgataatggt tacaggcctt ctgaaggtta ccttccagca gccgagcgca gaagtctgat
     1201 gttagctttt ggcgtcagtg gtgtgtcaat gtggcccagt gcttgagcga taatacacct
     1261 cactatagag gaatggaccc ttaactatca ctatagggga gagaccctaa gtccccacct
     1321 taatagcagt cacgacccat acacacattc attaagtctc aggcttccgg tagcggaccg
     1381 attggaaacc atccgcagac gcctagtgtt aaatcacctt ggcctgagac cattcctttt
     1441 aacgtaagct atcttccctt ttgacaggcg gggagcagta gaccacatcc gactttaagt
     1501 tggctctgtg ataatcgctg cgcggataac tgaagaacca agacaccatt actgaggtct
     1561 gccatccatt tgtggacagg cggaaactca aagaggttag atcagtggtg tctgaggttg
     1621 gagctttgag tcttgaccag caggctggag gagaccatga gtttatcttg ttatggattg
     1681 tgttatcaca attccaataa gaagaact
//
LOCUS       DAJTYF010000270         1702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTYF010000270 DAJTYF010000000
VERSION     DAJTYF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.276"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..444
                     /locus_tag="OO590_004243"
     CDS             <1..444
                     /locus_tag="OO590_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6639343.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPES
                     PAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVI
                     PLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            445..810
                     /locus_tag="OO590_004244"
     CDS             445..810
                     /locus_tag="OO590_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6639344.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            807..1523
                     /locus_tag="OO590_004245"
                     /pseudo
     CDS             807..1523
                     /locus_tag="OO590_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
BASE COUNT          485 a          353 c          464 g          400 t
ORIGIN      
        1 ccgggagagc agaccgccac cagcatactg aagcagcgca caaaaaatct tgcgccgctg
       61 cctcacgccc accagcaaca gaatccacca caggaagaga cggtggtcag cattgccgtt
      121 gatccggaat ctccggctca gtatctccag cgccagaaac cacaacggga agagatgcct
      181 gtatacacgc gctgggtaaa aacgcagaaa tgcatgacgt gtggcaatca ggcagatgat
      241 ccgcaccaca tcattggtca tggactggga gggatgggaa caaaggctga cgatttgttt
      301 gttattccgc tgtgtcgtaa atgccataac gaactacacg ccggggtaaa agattttgaa
      361 gaaaaacacg gtagtcagtt gttgttgctg attcgttttt taatgcacgc gagaaattcg
      421 ggtgttctga agtggaaagc ataaatgacc gaacgcatag aatttgtttt gccttacccg
      481 ccaacggtga acacctactg gcgacgtcgt ggcagcacat attttgtatc aaaagccggt
      541 gagcgttatc gccgtgatgt ggcgctaatt gttcgccagc agcgactgaa attaaacctg
      601 tccggaaggc tggcgataaa gattattgca gagccaccgg ataagcgccg tcgtgacctg
      661 gacaatatcc tgaaggcacc gctggatgcg ctgacgcatg ctggactact tatagacgac
      721 gagcagtttg atgaaatcaa tattgtgcgc ggtcagctcg ttcctggtgg gcggctgggg
      781 ataaaaatca cagaactggg gtgcgcatga ataaccagta tttacagttt gtgcgtgagc
      841 agctcattat cgccaccgct gatttgagtg gggcaacaaa aggtcagctt gaagcctggc
      901 aagagaatgc catgttcgat acagggcgtt acaggcgtaa aaaaatccgg taccgcgatg
      961 aagtgactgg aaaaatgata acgcgggata atccaccaat cccgggaaag caatcgctgg
     1021 cgaaggggac gtcaattcct ctggtcagtc cggttgagtt ttcgacatca tcgtggcggc
     1081 gggctgttct gtctcttgaa gaacatcata aagcctggtt gttgtggtgt tacagcggga
     1141 gtatttgttg ggaatatcag atcgcgataa cacagtgggc gtggaatgaa tttaatgctc
     1201 aatccggtac cagaaaaatt gcagggaaaa cgcaggaacg cctgaaaaaa ttaatctggc
     1261 tggcggcgca ggcagtaaaa gcagaacttt ttggtgggga aggttatgaa taccaggttg
     1321 gtggggaagg ttatgaatac caggagctgg catttactgg cgggagtgac aactaaaaac
     1381 tggtccaaaa catttactcg tcactggatt gcaatgaaac acatttttca ccgactggat
     1441 agtgaggctt tattgtttgt aatgagaaca cgttcaaaaa aaagacggca ttttcaaagc
     1501 aaagtgttgc aaaagtagat tgaaaggcat atattcatgc aaatctgata ttttgccgat
     1561 tttttacgtg atggcaaaag caaacaaaac ccgcccacaa gcgggttttt tgtgccactt
     1621 atctcggata gacatggtga atgcgctggt ggaggagata agggtgattt ttgaatgctt
     1681 gcaacattga tttcgtaacg tt
//
LOCUS       DAJTYF010000271         1701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTYF010000271 DAJTYF010000000
VERSION     DAJTYF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.277"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(605..941)
                     /locus_tag="OO590_004246"
                     /pseudo
     CDS             complement(605..941)
                     /locus_tag="OO590_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          551 a          338 c          329 g          483 t
ORIGIN      
        1 ttaatgtgtg tcatacactc agtggcgtaa tcgttgacaa tatggtgcat gtaatcgtaa
       61 gcgtccagcg aaatccatct ggtcataatc tgaagcatcc gacaaagtgg tgtccaccaa
      121 ataagtgatc tgcgcccaat atcctgagcc ggaactttga caaaggccaa cgaaaaatgg
      181 gagcggatgt tacagagttc tcggtacagg ggaaaagtgg tacctgtcgc cagttctccg
      241 atctgtttaa ccgggaaatt atctcctaca gcttgtcaga aaggtcggtg atggagcatc
      301 gttaatacga tgcattcaca gtgcccggcc agaggatgcc ccgtcgctgc atatggatca
      361 gggttggcaa tatcgaatcg caggctatag gcaaagttaa agccccatgg agtagcacaa
      421 aatatgccgc acaaaggaaa cggtctgaat aacgcagtga tgaagaactt cttcagcaca
      481 ctgctaaaac gcatagtgat cgagcgctga ttctggcgaa caactgaact aatacatcag
      541 aatctgcatt atgttaaata aatatgaaaa gatggtttaa ataccccgtt acttttgact
      601 tacactatac ggtatcgcat cgtttaatat tcgcaccggc cagattttta tttctattag
      661 ttgtcacaat actgaatgcg tacgaccaca gtattctggc tcctgtgtgg ttatgcttta
      721 attctgcgtt ccgggcagat aagcagttgc ttgcaggaat ccttcttgtg ttaatgtcag
      781 ttcccctttt taccagtgct gatttccaca ttccgtccaa cagagcttat agcctttccc
      841 tggattatag cattgtccgg ctgaagttct ttttgaataa taatagaagc actgctggca
      901 gatccagtcc gtttttcata acccactgta ctgataacca taatctaatc agtagaaatt
      961 gagtcgaaaa taagcactac tccatacagg ataattagag gtcagtttga ttattcacaa
     1021 ttcatcatca gcattttcta tttctgacga aatcaacatg aaaataacca tatatgataa
     1081 ttattataat aacggcttta attggaatac atatattaca acgtattata tataattggt
     1141 attctgggaa ctatattctc aaaatacagt agaaacgagg tatgtttctg gtggaaagga
     1201 cagtgggatt aaaaagtaag aattgataaa aaaaacgcca gcaacacata gtgccggcgg
     1261 agggaatacc ccatggagaa aatgtgatgc ctagagcatc atgaatatat aattaaaaat
     1321 agttagcgtt gtcaatactt tcacaaaaat aattttcgta gtatagaaag atatttttat
     1381 gcatgaccta cctgaatttg ctccgggtag aggttataaa taaaaattga atcacgacaa
     1441 acacaatatt catagtatgg cgatgcctac gccagcaaga atagcgccaa taatattggg
     1501 aatataatag acaccagacg cacaggcatc tcactcctta acaaacaata atcaggatat
     1561 ttacttttac ccagctaact gtttacaccc aaagtgcaca caattaacca ttcaataaca
     1621 aatggcaata tccatagcca tacgacttta ccttgtaatg ttcggtattt ctttataatt
     1681 attctgggaa atctatcatt t
//
LOCUS       DAJTYF010000272         1681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTYF010000272 DAJTYF010000000
VERSION     DAJTYF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.278"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            421..>1681
                     /gene="pykF"
                     /locus_tag="OO590_004247"
     CDS             421..>1681
                     /gene="pykF"
                     /locus_tag="OO590_004247"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6639345.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLV"
BASE COUNT          459 a          421 c          392 g          409 t
ORIGIN      
        1 ggatgaactt tcacttatcc tcacactgac aacttcggca ccagacgttg cgcaaacagc
       61 gaagtttttg cgtaaccttt tccctggaac gttaaatctt tgataacaat ttattgtcta
      121 acaagttgta tattttttga aacgctgttt ttgttttcct tttggattaa tttcagcgta
      181 taatgcgcgc caattgactc ttgaatggtt tcagcacttt ggactgtaga actcaacgac
      241 tcaaaaacag gcactcacgt tgggctgaga cacaagcaca cattcctctg cacgcttttt
      301 cgatgtcacc tatccttaga gcgaggcacc accactttcg taataccgga ttcgctttcc
      361 ggcagtgcgc ccagaaagca agtttctccc atccttctca acttaaagac taagactgtc
      421 atgaaaaaga ccaaaattgt ttgcaccatc ggaccgaaaa ccgaatctga agagatgtta
      481 gctaaaatgc tggacgctgg catgaacgtt atgcgtctga acttctctca tggtgactat
      541 gcagaacacg gtcagcgcat tcagaatctg cgcaacgtga tgagcaaaac tggtaaaacc
      601 gccgctatcc tgcttgatac caaaggtccg gaaatccgca ccatgaaact ggaaggcggt
      661 aacgatgttt ctctgaaagc tggtcagacc tttactttca ccactgataa atctgttatc
      721 ggcaacagcg aaatggttgc ggtaacgtat gaaggtttca ctactgacct gtctgttggc
      781 aacaccgtac tggttgacga tggtctgatc ggtatggaag ttaccgccat tgaaggtaac
      841 aaagttatct gtaaagtgct gaacaacggt gacctgggcg aaaacaaagg tgtgaacctg
      901 cctggcgttt ccattgctct gccagcactg gctgaaaaag acaaacagga cctgatcttt
      961 ggttgcgaaa aaggcgtaga ctttgttgct gcttccttta ttcgtaagcg ttctgacgtt
     1021 atcgaaatcc gtgagcacct gaaagcgcac ggcggcgaaa acatccacat catctccaaa
     1081 atcgaaaacc aggaaggcct caacaacttc gacgaaatcc tcgaagcctc tgacggcatc
     1141 atggttgcgc gtggcgacct gggtgtagaa atcccggtag aagaagttat cttcgcccag
     1201 aagatgatga tcgaaaaatg tatccgtgca cgtaaagtcg ttatcactgc gacccagatg
     1261 ctggattcca tgatcaaaaa cccacgcccg actcgcgcag aagccggtga cgttgcaaac
     1321 gccatcctcg acggtactga cgcagtgatg ctgtctggtg aatccgcaaa aggtaaatac
     1381 ccgctggaag cggtttctat catggcgacc atctgcgaac gtaccgaccg cgtgatgaac
     1441 agccgtctcg agttcaacaa tgacaaccgt aaactgcgca tcaccgaagc ggtatgccgt
     1501 ggtgccgttg aaactgctga aaaactggat gctccgctga tcgtggttgc tacccagggc
     1561 ggtaaatctg ctcgcgcagt acgtaaatac ttcccggatg ccaccatcct ggcactgacc
     1621 accaacgaaa aaacggctca tcagttggta ctgagcaaag gcgttgtgcc gcagctggtt
     1681 a
//
LOCUS       DAJTYF010000273         1595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTYF010000273 DAJTYF010000000
VERSION     DAJTYF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.279"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..685)
                     /locus_tag="OO590_004248"
     CDS             complement(<1..685)
                     /locus_tag="OO590_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6639346.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQ"
     gene            complement(1001..>1595)
                     /locus_tag="OO590_004249"
     CDS             complement(1001..>1595)
                     /locus_tag="OO590_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6639347.1"
                     /translation="WWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHLDIGVGTGFYLT
                     HVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALHGQFDSISMFYL
                     LHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFS
                     NTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          468 a          296 c          333 g          498 t
ORIGIN      
        1 gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga ttgacaacgc
       61 tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc tgattaagcc
      121 agaatattct tataatatga tcatctcttt ctggaaactc tggtattact tccaaagcat
      181 ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca gacaggtgat
      241 tgtcacttac attaatatat tctagtgacg caggtaattc aggaagtgcg aacaaatggt
      301 tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca gataattgat
      361 tactgcttgc attcagctct ttcaatgccc ttggtagctc ggggagcatt gatagttggt
      421 tattgcttac attaatttca tcaagattgt caggcaatcg tggtagagat ctgagaccta
      481 aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc cgggtaaaag
      541 cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat tcggagagat
      601 aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt ccagtggata
      661 gggggggatt atttacagga agcataataa cctcgcagaa ggatatcctg ataaaatgtg
      721 atactgggga ataataagct gaaataaaat gtttcaggat tgaaatttat tgttttcatt
      781 ttaaggaagt gatgttgatt taactgtaaa aaaacgatac tattttctta gatggtaaat
      841 gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt catatttgaa
      901 cgttctgccg gaatgcatta tcaatagagg taaagtcgca accccaaatc gtaaaggaaa
      961 ccgtagcacg tcttaagcaa gaacgtgctg cggttggatg ctattttttc cctgaagcgg
     1021 aaaacattac tacagtacct tgaaccttgg ttttaacatt ctcgaaatgc tctgagagta
     1081 tatgtgttaa gccttcttcg gaatcttttg tgtttgaaaa gatgcctttc tgattgtaaa
     1141 tgcgcatcag tttttgaccg aagctattgt gcacaactcc atcgccaaga attgtggctc
     1201 cgtatagagt tccatcatca gttaaggcct gcgccgcatt gcgtattaca cagctttttg
     1261 tagatatatt tccaggcagg cagtgaagaa ggtaaaacat ggaaatggaa tcaaattgac
     1321 catgtaacgc cgcaggataa ggttcaaaaa catcatggct aattttatgt ttaatttttg
     1381 attccccagc ccttgtagat gccgcgttca ggctagcttc gttcaaatcc attaaagata
     1441 tcagactact ctcaggtacg tgagtaaggt aaaacccagt tccaacacca atatccagat
     1501 ggttgttacc taaatgttcc agaaagtgtg gaagaaggtg ttcctttgta ggacatcccc
     1561 atgcaagccg atttgatact cccaaaaccc accag
//
LOCUS       DAJTYF010000274         1553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTYF010000274 DAJTYF010000000
VERSION     DAJTYF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.280"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(325..480)
                     /gene="hokD"
                     /locus_tag="OO590_004250"
     CDS             complement(325..480)
                     /gene="hokD"
                     /locus_tag="OO590_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6639348.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1132..1548)
                     /locus_tag="OO590_004251"
     CDS             complement(1132..1548)
                     /locus_tag="OO590_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639349.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
BASE COUNT          384 a          391 c          327 g          451 t
ORIGIN      
        1 cgtgtctgcg gtagcgctga atcagccatt cagcctgttc ttcggtgcag gagtcgtgct
       61 gataccagtc agatttgaat gcatgagaac gccgcccgtg cctgctggca ggggcggcag
      121 agttatccga attgtaaaat ttggtatcgt gcgccatctg ttttctctgc tggcgcagca
      181 ggtgccagtt gttcaggctg acggatggat tgtaaaccag aacgaccaga aaaaacaaaa
      241 cccgccgaag cgggttaagt gcgggtgcgt tgaggatgcc tgacacatca gcggtggcga
      301 gggatttctc ccccgccggg tctcttactc ctcaggttcg taagctgtga agacagcgac
      361 ctccgtctgg ccggttcgga ttcgtacctc gcagaggtct ttcctcgtta ccagtgccgt
      421 cactatgacg gttaaacaga tgacgataag ggcgattaac atcgcctttt gctgcttcat
      481 agcctgcttc tccttgcctt tcggcacgta agaggctaac ctacatgtgt tcagcatgga
      541 ttgagcctca gattaatgtt aagcgtcttg caggacgcgt aatgttaact ggggcttttc
      601 tctatctgcc ttttggtgtt catgcctgag acagatagcc tcaagcaccc gcagtcattc
      661 tacttaacta agatttccct gcaaaccgtt tttgtccggc acagtaaata tccaactaaa
      721 ccaatagcgt tcgctgtatt taccgccagt attcaatgca catgaccgcc atgaacaccc
      781 ctaaaaaaag ggcatttata tgtccaaaca ttaatatcaa aacatcaatt ttttccatat
      841 accttgctgt gaagatgatg ggcatacatg atgcgaacaa ccagaacgca acaaacaaaa
      901 actgcaatgc gtttttcatt attcccccta caatcaatgt gcaataacat ttaaacacac
      961 ctcaatttgg ccggacatat aaatatctaa accagaaaaa atcacttaca tagcgttaca
     1021 aactctttag tctaaatact catcgtaaaa cattccccat acttatcagt ccgttccgcg
     1081 ccaggtagct tattgcctta tctggcaacc tataatcagg tttccgcttt ttcagttggc
     1141 tggtcgttta accgacatag ttaacccatt aatctggttg ccggatgctg gtggattttc
     1201 gcgttttagt tgttcataaa agtgcacagc tttaaccagt tcttctgatg taaccgggac
     1261 tggtggggca gtgaataagg cctgaatttc atagttcggc ctgtcgttac aatcctcttt
     1321 tttcggtaca tatttccagt caccaaccca caactccccc tgagagtcca taacaccttt
     1381 tttcacgtag cgatatcgcc acgctatcgg ctctgcttcc agcgatgccc gtgcaatttt
     1441 gaataactcg ccctctactc gcgccatccc tgaattgggg tggcatttcg taatcgctat
     1501 ttttaatttg gcttcttcga ttaattgttc ttttgttaat tcagtcattt tca
//
LOCUS       DAJTYF010000275         1547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTYF010000275 DAJTYF010000000
VERSION     DAJTYF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.281"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..864)
                     /gene="mdtL"
                     /locus_tag="OO590_004252"
     CDS             complement(<1..864)
                     /gene="mdtL"
                     /locus_tag="OO590_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6639350.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVS"
     gene            complement(996..>1547)
                     /locus_tag="OO590_004253"
     CDS             complement(996..>1547)
                     /locus_tag="OO590_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6639351.1"
                     /translation="GSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          466 a          396 c          401 g          284 t
ORIGIN      
        1 tgaaacggca agggtgatcc ccgccgcaag gaataacacc tgcgaggtga tcatcaacgt
       61 acgtggctta aaaattccca gcgcaaatgg cgtcgagaat gaaaaggtca tgctgacgcc
      121 agcggtcagc gccataatgg tggcgtattc tccgcgctca aaccccatga tttccatcag
      181 caataccggc gatgtattga cgaaagtgag gatcaccgaa acgctgaggg tggtgataac
      241 aacacggctg aggaaaaaac gattaagcag cgactcgcta ttttctcgtg gtttgtccga
      301 agcagcgggg gacgctgggc gcgtttcttt taaaataaac aaagacaaca tcagtaccgc
      361 gatgcccatc attgccatcg cccagaacag actctgccac gggaatttaa gcataatcag
      421 atgtccgagc actggcgcta acaccggaat gatgcaggta ataccgttga gtaatgacag
      481 cactttagcc cgacgtcgat catccagcgt gtcgcgcaaa atggcgaacg ccaccacgta
      541 acaacagcct gcgcccaacc cctgtagaaa tcggcctgca agaaataacg tgctggtttc
      601 agccagtgaa cagaacaccg aggcaataat aaatagcgcc gcgccgggta tggcgaccgg
      661 ctttctccct gaacgatcgg ccactttacc ggcaaatagc atcgcagctg ccatccccgc
      721 cagatatacg gagaacgcaa tatgcaactg cgcttcgctg gcattgagat cggcggcgat
      781 gcgcggtaaa ccaacgaggt acatatcaat cccggcggga taaagtaaaa ccagggcaaa
      841 actacaaatc aaaaagcggg acatgggcta aagcgtcctg atagtaaggt gacgcaaagc
      901 atacgtggtg aaggaggata aggcgagttg ccatttggac ataagtgatt tcctgagagg
      961 caagaagcca gcgaatggct ggcttcttga aggatttagc caaatttagg taacacgtta
     1021 aagacgttgc cgaaccagca caaaatcacg gttataccaa agagaatgac aatcgccgga
     1081 ataagattgc cgccccagac cgtgaacatc tgattgggaa acttcttgcg agctttgatt
     1141 gcaagcactg cgggaataat gaccgcccag atggtggcgc acagcccggc accgccgatc
     1201 ccgtaaataa agccgttcgg gaagatcaga tacaacaacg caggtggcag gaaggttaac
     1261 agcacggttt tgaaacgccc gccgtgggag ttatcaatct taaacaggtc cgccagataa
     1321 tcgaacaacc ccagcgtgac accaaagaac gaactggcaa cagctaagtt agagaacacc
     1381 agcaggcaaa actcgataat gccgtgctgt ttggtgccga ggaacgattt caccagcgaa
     1441 tcaacgttgc cgcctgagga gattatcgcc ttaaagcttt cgcgcggaat attccccatc
     1501 gtgcaataga gccagaagag ataaatcacc agcgccagca gcgaacc
//
LOCUS       DAJTYF010000276         1537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTYF010000276 DAJTYF010000000
VERSION     DAJTYF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.282"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(71..538)
                     /locus_tag="OO590_004254"
     CDS             complement(71..538)
                     /locus_tag="OO590_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6639352.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(604..843)
                     /locus_tag="OO590_004255"
     CDS             complement(604..843)
                     /locus_tag="OO590_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR6639353.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(978..>1537)
                     /locus_tag="OO590_004256"
     CDS             complement(978..>1537)
                     /locus_tag="OO590_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6639354.1"
                     /translation="YPASRRITTSTGNVINLSENGYRFLLLLLQGESDKQHIINEVWR
                     EQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRYLGKVEKHVCENERDSD
                     EDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTDKNIEWYRTKQWSVFIS
                     VLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          491 a          338 c          276 g          432 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaagcat ccgatgattc atttatgctg
       61 tcctaaaaaa ttaacaaaac aatgggaaaa caggatatgt aagcagaaaa gccaatgaaa
      121 ttggtacagc ataaggcaca ccgttttcac gaattgattt tcgaaaaaaa agaagacctg
      181 ccagaacaac cagagcgccg caaaaggtca ttgcgacgag aaaattagcc agttcatgtt
      241 catcaatagc taacgaaaga acagttaaca gcttgacatc accgccgcca attatccctg
      301 cggaaaaaag aaagaagccc acgagcaaaa cgctcccggc tgcaaccaga ttgggttttt
      361 gatgctgtaa aatgagtgcc aaaaaaataa gcacggttaa caccagtaaa taacgatggc
      421 taataattct gagtaatata tctccccatg aaataaaaat aagaaagagc gcaacaaata
      481 aaagatcgac tcttagtaac catattgctg ccattgaaag actccatgta ccatacaagg
      541 ttgctgccag acatattcct ttacgtctga cagcctctag agagtttttt tattttttat
      601 aagttacgga ttactcgttc cggaaatcgt tacctgttga attgctgcag cgatagcgtc
      661 gaatgcatct ttaattgctc ccaggaagcc tgagttctga tcgccaaaga tgaaagcaag
      721 cagagttgcc atagcaacac caatcaatgc atattcgatt gcagtaacac cacgttcatc
      781 cttcgcgaaa cgcgcaaagc actgtttagt tttaccatag tacttagtaa taaaagtatt
      841 catgttttcc tcgtatatta ttatttaaat tcaaaccacc agcttcatct gcgtgtcacc
      901 ggtatcccat atttttggga tgtggctata attatccatt cccgctattt agtcttcaaa
      961 aaaaattaca tttaatatca tttatcttga ttaagaaata gtaagactaa aaaaacaagc
     1021 gtcgtcagcc agcacactgc taacacggta agaacgctaa taaaaacact ccactgcttc
     1081 gttctatacc attcaatatt tttgtcagta tgttttattg ggagcagctc ttcagtagag
     1141 aattcctggc tgttatcatt ctcaggaatg tttgcatcag tgtttacttc cctggcctcg
     1201 ctatcgttgg tatagctact actgtcttca tcactgtcac gctcattctc acagacatgt
     1261 ttctcgacct ttcccagata tctgacgcct tttcgcggaa tggttttaat tagagaactc
     1321 gacaaacctg cgacgtctaa tgcctttcta agcatgtaaa tctgcccata ataactgctg
     1381 tcgcttaccg aacctttctg ctcccgccaa acctcattga taatatgttg cttatcggat
     1441 tctccctgta gtaaaaggag taaaaatcga tagccgtttt cagacagatt gattacattg
     1501 cctgtactgg tggtgatacg tcgagacgct gggtaaa
//
LOCUS       DAJTYF010000277         1526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTYF010000277 DAJTYF010000000
VERSION     DAJTYF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.283"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            101..730
                     /locus_tag="OO590_004257"
     CDS             101..730
                     /locus_tag="OO590_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6639355.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(869..>1526)
                     /locus_tag="OO590_004258"
     CDS             complement(869..>1526)
                     /locus_tag="OO590_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6639356.1"
                     /translation="YYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILW
                     AGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDYGE
                     YKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQFIMI
                     ALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA
                     "
BASE COUNT          395 a          390 c          377 g          364 t
ORIGIN      
        1 cctggcagca tgctgccagg tgctgggcca cctcttttgc gattgtaatt tcatgcttgc
       61 tggtgaatag tcagtatttt ccctgacttg cgaactcacc atgacccgca cactcaagcc
      121 gttaattctt aacaccggcg cactggcgct aacgttaatc ctgatttata ccggcatttc
      181 ggcccatgac aaactcacct ggctgctgga agtgacaccg gtgattatcg tcgtgccgct
      241 actacttgcc accgccagac gttacccgtt aacgctgctg ctctatacgc tcatcttctt
      301 tcacgccatc atcctgatgg taggcgggca gtacacctac gcgaaagtcc ccgttggttt
      361 tgaggtgcag gaatggctgg ggttgagtcg taatccgtat gacaagctgg ggcacttttt
      421 ccaggggctg gtgcctgcac tggtggcacg agaaattctc gttcgcggga tgtacgtgcg
      481 cggacgtaaa atggtggcgt ttctggtctg ttgcgtggcg ctggcgataa gcgcgatgta
      541 tgaattaatc gagtggtggg cggcactggc gatgggccag ggagcggatg attttctcgg
      601 tacccagggc gaccagtggg acacgcaatc cgatatgttc tgcgcgctgc ttggcgcatt
      661 aacgacagtg atattgctcg ctcgctttca ttgccgccag ctacggcgct atggcttgat
      721 aaccggataa caacgtgcac tttgttgatg ccggatgcgg cgtgaattcc ttatccggtc
      781 tacaaaattt gcaaatccaa agcattacat taagacgtgg cctgataaac atatcgcatc
      841 aggccatttt cgttagctat tgtccacgtc aggctttcac atcactcact gcaccgaccg
      901 gaatatcagc atgaacaggc tcgggcggta ctttgcgata tttatccagc agatgaatct
      961 ggatcctgcg cagcgtgtcg ccattgaggc gatagaaacg gaagtagaga atcagcgtta
     1021 ccatgaaaaa cagagtcggt agagcaatca taataaactg catacctaat agggcttgcg
     1081 tagactgttc aacattcggt acatagccaa tcatccctaa cacaaccgca atgaaaaaag
     1141 ccgcaaaggc tgaaccgccc ttcaccacca tagtctgcac ggagtaagcg atgctttcac
     1201 agcgtacgtg cagtttatat tcaccgtaat caacggtatc tgccaccatg atgacctgta
     1261 atacccagaa aagcgccgtt cccacattca gcaaaatacc cgcaatcaca atgaggacga
     1321 cgttgtgata gctcattaat gccattaaca ggagaacacc acagcttaac accggaagaa
     1381 tagatgctcc ggcccataaa atgcgtcggg ataatgattt aactaagcgt gggaagaata
     1441 ctaacgtcac caggttagca gctcccgcat acgacagata ataggggaac aaatccgcat
     1501 caccgataac atatgagaaa taatag
//
LOCUS       DAJTYF010000278         1485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTYF010000278 DAJTYF010000000
VERSION     DAJTYF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.285"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..787)
                     /locus_tag="OO590_004259"
     CDS             complement(<1..787)
                     /locus_tag="OO590_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6639357.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSL"
     gene            complement(1388..>1485)
                     /locus_tag="OO590_004260"
     CDS             complement(1388..>1485)
                     /locus_tag="OO590_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6639358.1"
                     /translation="VTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          396 a          240 c          361 g          488 t
ORIGIN      
        1 gcagggattg cagagcatgg gatgacaatg ggttatcact aatatctatt gaacattcat
       61 tcctcagatt aagaatgctt tccgggatat gacttatctg atttatgtca aaataaccgt
      121 tccttaaatt ttccggcagg tatggaatat cggttaacat attgtcgatt gcccatatgg
      181 agtgaatgtt gtgaggtaga ggggggagtt cactaagaag attccctatt gcgctaatct
      241 cttgtaaaga aaatggtaaa ggtggtaaaa ctgccagccc attacatgat acatctaatg
      301 tttccagtag ttctggtaaa acaggaagag aacataattg attatttgag acatgaagct
      361 ccttcaaaga tatggggagt gtgggtagtg tgattagttg attgtgggac gcattcaata
      421 atttaagtcc ttgaggcaaa gcaggcagtt caataagtct gttatagctg acattaagtt
      481 gtgtaaggga cgcaggcaat ggggagatta agcttaaatt atttttactt atattaattg
      541 atttaattcc cggggggatt tcaggtaatg ttgtcaggcc taattcagac aagtccaggt
      601 tcgtctcttg gttttgtaga catgatacta gtcgctgaaa agcgatgtct cgttgttctt
      661 cttgtatgcg gttgtttttc cattcagtcc aatgggttag ataattttca tatgcgcggc
      721 tagtgtcgat tgaataagtg gagaacgaat ttgaaattaa tctgtgattg ttatttgtcg
      781 ggagcataaa tatcagggcg tattctttat gttgagaggt gttggattct tttttatctg
      841 ctttgcaatg ttattgattc ccttcagcag tagacaggaa cttctggagg aggcggatat
      901 atagtttgtg taagcatatt aactccatat gttatatatt gaagaacttc tgctttacac
      961 tatcctaaac tggatcggtt taatgtaaaa aaacgatact ttttggggga gggggcaatt
     1021 tttccttaag gtatgaaaaa aggagctgaa gctatataat agctattaat gccactggta
     1081 tcagtgaatc atgtatataa aaaactgtgt taaggaggct gtccattagc ggttttttat
     1141 tcttctggaa ccgcggcatc tttgcgtatc tttttgtctt ctcaccgtct ggtcggtgtt
     1201 ttgctgagac tgctaatttc ctgtctttgc tagtgctgta ctggtactgc ccaatcatga
     1261 ttggcggggg cgggcggagt tgagaccgca gacacgtcgt atgcaagaac gtgctgcggc
     1321 tggctggtga acttccgata gtgcgagtat tgaatgattt ccagccgtta ctgattttac
     1381 gtgctaatta gtgaacaaac cactcgtcag cagactccca ggtatctttc agagtttcct
     1441 gaacaaaagt tttagctgaa tctttatcgg cggtgcgcgt aacag
//
LOCUS       DAJTYF010000279         1474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTYF010000279 DAJTYF010000000
VERSION     DAJTYF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.286"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..546)
                     /locus_tag="OO590_004261"
     CDS             complement(<1..546)
                     /locus_tag="OO590_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639359.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AVTDKSYLSLNPDKYPNYRFLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..>1474)
                     /locus_tag="OO590_004262"
     CDS             complement(1208..>1474)
                     /locus_tag="OO590_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639360.1"
                     /translation="DVAYYNGADRGVFMMAKRFTALSFGCFRHDGENITVVLSRDGNT
                     QSHDLEILGKVFVVAEAATTVKMGTGSAAAVLPVVKRLSFTKTS"
BASE COUNT          426 a          316 c          323 g          409 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaaaacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgtta ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggagccgag gacaactagc taacttcttg tttcttaaag actgatgaat ccagtggtta
      841 cagtgatggt tacacatgga gaggctaaag gtcaagactt gaagtacgta acattcccac
      901 aaaaggaata gaacacagtc acggactacc cggccctttg gtctgactga gggtcgggct
      961 acggggtaaa tcgtcaggct tattgtatta gatggtctct cacatttttc agtcaaaaac
     1021 tcatcgtact ttaggccaga ccatcagcat gactatcaga gccagcttta gcaggaagac
     1081 cacgagatag actttgtgta ggtccattag gtcctcctct ttccgtaccc atgaactaga
     1141 ttactagtac aaaataaagg ccagacatcc agttacggaa gtctgaccaa taagtcgtta
     1201 ttgttagtta gcttgtctta gtaaagctca ggcgtttgac tacaggcagg acagcagcag
     1261 cgctgccagt ccccatctta acagtcgttg cagcctcagc gaccacgaag accttaccga
     1321 gaatctcaag gtcgtgagac tgggtgttgc catctcggga taagaccaca gtaatattct
     1381 cgccatcgtg acggaagcaa ccgaagctca gagccgtaaa gcgcttagcc atcatgaaga
     1441 cgccacggtc tgcaccgttg tagtaggcga cgtc
//
LOCUS       DAJTYF010000280         1460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTYF010000280 DAJTYF010000000
VERSION     DAJTYF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.287"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(506..>1460)
                     /gene="ychF"
                     /locus_tag="OO590_004263"
     CDS             complement(506..>1460)
                     /gene="ychF"
                     /locus_tag="OO590_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6639361.1"
                     /translation="PDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQF
                     LTNIRETEAIGHVVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQ
                     KKAKGGDKDAKAELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIA
                     NVNEDGFENNPYLDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEP
                     GLNRVIRAGYKLLNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTI
                     SFEDFITYKGEQGAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT          355 a          346 c          329 g          430 t
ORIGIN      
        1 cgcttgatgt atctgcataa caaaaacaat atgcgccatt tttgcattac taataagaaa
       61 aagcaaaaaa catcgatatt catttttggt caatggattt tctggcctat cgttattatt
      121 gtgctactgg ttatcatttt catcttgcca ggatcgcacg taatcacgtt attcggggac
      181 tttatctgcg ggatattacc cttcaggtaa gtccttttct cgccagcata ttggagcgag
      241 atcaatattg atcagctgtg aagagggctg atgtgtagtg gaacaaaaac tgacgttata
      301 tcactgctaa tgagatatca gtcatctgaa cacttatgtc tttaattatt ctcgtggttc
      361 actgtaggca ataagcacaa aagtgtagga tgttacaaga atgattagga ctcggtgaaa
      421 tgaaaaatcc acgcaattgc gtggatttta tatacttttg cgctcttcat gagatttagc
      481 gaaacctcat gagacaataa attaattaga cgttgaaaag gaagttcatc acatcgccat
      541 ctttcacgat gtaatcttta ccttctgcac gcattttgcc tgcttctttc gcgccttgtt
      601 cacctttgta agtgatgaaa tcttcaaacg agatggtttg tgcacggata aagccttttt
      661 caaaatcagt atggattttg cccgctgcct gcggcgcggt tgctccaacc ggaatggtcc
      721 atgcacgcac ttctttcacc ccagcggtga agtaagtttg caggttcagc agcttataac
      781 cggcacggat cacacggttc aggcccggtt cttccagccc aagctcctgc ataaactcgt
      841 cacgttcttc gtcgtccagt tcggcaatgt ctgcttcaac agcagcacaa accggaacca
      901 caacagaacc ttctttcgcc gcgatttcac gcacctggtc aagatatggg ttgttttcaa
      961 aaccgtcttc gttgacgttg gctatgtaca ttgttggttt cagcgtcagg aagctcaggt
     1021 aacgaatagc cgctttctct tcagcgctta aatccagcgc gcgcagcata cctgcgtttt
     1081 ccagctgggg caggcatttt tccaggaccg ccagctcagc tttcgcgtct ttatcgccac
     1141 ctttggcttt cttctgtacg cgatgaatcg cacgttcgca ggtgtcgagg tctgccagcg
     1201 ccagttcggt gttgataact tcaatatcgt cagccgggtt aactttgccg gaaacgtgaa
     1261 tgatgttgtc attttcaaag cagcgaacaa cgtgaccgat cgcttcggtt tcacggatgt
     1321 tggtcaggaa ctggttaccc agaccttcgc ctttcgatgc gccttttacc agaccggcga
     1381 tatcgacaaa ttccatggtc gtgggaagcg tacgttgcgg ttttacgatt tcagccagtt
     1441 gatccaggcg agggtcaggc
//
LOCUS       DAJTYF010000281         1425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTYF010000281 DAJTYF010000000
VERSION     DAJTYF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.288"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..1331
                     /locus_tag="OO590_004264"
     CDS             <1..1331
                     /locus_tag="OO590_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6639362.1"
                     /translation="GDAKEIISIKNSIVEMKDAEIERSNKLLSLISYDQESGFIKNMA
                     IIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNA
                     DLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKL
                     AISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAI
                     LSAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKD
                     DFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQ
                     LRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARY
                     NRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT          411 a          278 c          343 g          393 t
ORIGIN      
        1 aaggcgatgc gaaagagatt atcagcatca aaaattcgat cgtggaaatg aaagatgccg
       61 aaattgaacg gtcaaataaa ttgctctcac tgatctctta cgatcaggaa agcggtttta
      121 ttaaaaatat ggcgattatt gagtccaaca ataatcagta tctggctgtg gggatcatca
      181 aactgtgtgg tctggaagcc gtggaagcgg tgtttggtgt tgatgaacgc aataaaatcg
      241 tcaggaaatt gtgtcagcga attgccgaga aatatgcgca atgctgcgat atcgtgacat
      301 tcaatgccga tctctattta cttctgtgtc gggaaaatgt acagacattt acccgtaaaa
      361 tagcgatggt aaacgatttt gacagcagct ttggctaccg caatctgcgc atccataagt
      421 ctgccatttg tgaacctttg cagggggaaa acgcctggag ttacgcagaa aaactgaaac
      481 tggcgatttc cagtatccgt gaccatatgt tctccgagtt tattttctgt gatgacgcga
      541 aactcaacga aatagaagag aatatctgga ttgcgcgtaa tattcgccat gcaatggaaa
      601 ttggcgaact attcctcgtc tatcaaccga tcgttgatat taacacccgc gccattctga
      661 gcgcggaggc gttgtgccgt tgggtttctg cggagcgggg gatcatttca ccgctgaagt
      721 tcattaccat tgctgaagat atcgggttta tcaatgagct gggttatcag attattaaaa
      781 cggcaatggg tgaattcaga cattttagtc aacgtgcgtc gctgaaggat gatttcttac
      841 tgcatattaa tgtttcgccc tggcagttaa acgaaccgca ctttcatgag cgttttacca
      901 ccatcatgaa agaaaatggc ctgaaggcga acagcctctg tgttgagatc actgaaaccg
      961 tgatcgagcg aattaatgaa catttttatc tcaatattga acaactgcgt aaacaagggg
     1021 tacggatatc gattgatgac tttggcaccg gtttgtcaaa cctgaaacgt ttttatgaaa
     1081 ttaatccaga cagcataaag gtggactcgc aatttaccgg cgatattttc ggtactgcgg
     1141 gaaaaattgt gcgcattatt ttcgatctgg cacgctataa ccggatcccg gtgattgcgg
     1201 aaggcgtaga gagcgaagac gttgcgcgcg aattaattaa attaggatgc gttcaggctc
     1261 aggggtatct gtaccagaaa cccatgccat tctccgcctg ggataaaagt ggaaaattag
     1321 taaaagagta gtttacgtat gtccagaatc aataagttcg tacttacagt cagtctgctg
     1381 atttttatca tgatttcagc agttgcctgc gggatctaca ctcaa
//
LOCUS       DAJTYF010000282         1389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTYF010000282 DAJTYF010000000
VERSION     DAJTYF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.289"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(1..572)
                     /locus_tag="OO590_004265"
                     /pseudo
     CDS             complement(1..572)
                     /locus_tag="OO590_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(620..1114)
                     /locus_tag="OO590_004266"
     CDS             complement(620..1114)
                     /locus_tag="OO590_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6639363.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          423 a          280 c          263 g          423 t
ORIGIN      
        1 atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc ttcccggcgc
       61 catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg tcgccagaac
      121 agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt gctcgctgat
      181 ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg caatgacaca
      241 cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg tgatactggc
      301 ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc
      361 tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta
      421 ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt
      481 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta
      541 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaattgaa
      601 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      661 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      721 ccttgccttc agcatcgata gcggtataat cagagtcgtt ataaagttta acctgcgaat
      781 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      841 catcaaaacg aacggctgct tgagtgtacg ttgccgggca attttcaagt ttaatactga
      901 acttctgtgg cgacgccaga ctacctacac ctgaaaatgc atttttactg actttaccca
      961 gtgggacaat ttgatcggct gtatcggagg taacttcaca agtagtgtcg acaatttcac
     1021 cgacgaagtt aacaacacca tcttctgcca gtgcatgccc ggccagcatt agagatgcca
     1081 gagtgaatgc accgcctata atattacgct tcataaaaac tccattgata taatatatcc
     1141 tttaccatga atcatcactt atattaataa gtgtattttt gattcaaagg aatagtacac
     1201 caaagagacg gctttatcta aaaataatat taattacgca aaaataaaat atagatttat
     1261 tgtttaaata ttatttaaac agtaaaaaat caagtttgtt atcagacatt aatgataatt
     1321 cagaatatat ttatatagaa agatagatga atttattgat gtcagttata aaaaagggcc
     1381 accggtcgg
//
LOCUS       DAJTYF010000283         1356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTYF010000283 DAJTYF010000000
VERSION     DAJTYF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.290"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            193..462
                     /locus_tag="OO590_004267"
     CDS             193..462
                     /locus_tag="OO590_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639364.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..685
                     /locus_tag="OO590_004268"
                     /pseudo
     CDS             459..685
                     /locus_tag="OO590_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="OO590_004269"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="OO590_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR6639365.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          389 a          263 c          333 g          371 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgaaagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagccgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat ggggac
//
LOCUS       DAJTYF010000284         1346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTYF010000284 DAJTYF010000000
VERSION     DAJTYF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.291"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..384
                     /locus_tag="OO590_004270"
     CDS             <1..384
                     /locus_tag="OO590_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR6639366.1"
                     /translation="RCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            381..>1346
                     /locus_tag="OO590_004271"
     CDS             381..>1346
                     /locus_tag="OO590_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR6639367.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLA"
BASE COUNT          301 a          324 c          413 g          308 t
ORIGIN      
        1 cgttgttatg tggcacttga agagcttcgc tggggcgcgt ttggtgatgt gatacgtcag
       61 ggcgaaacag gccaggttaa ccagctactg gatattctca gacataaagc attaacgcag
      121 atggcacagg agagtggcgg tagtgcaacc gtgcgtctga acactctgga ctggcttggt
      181 ggtcagggcc gggagcaggc agataacgag tggcatgacg cgattaactg gctgggggac
      241 tggtgcagtg aagagcagca tccggtgatc tggtcaacca cgcaggctgc agaacactta
      301 ccggttcgca tgccccgtct ctgctctgca gaacgcctct ctgaaagtat ggtcgatgaa
      361 atatttcaga agggggctgc atgagtatag aaataaaaac agggctggtg ctgtccggcg
      421 gtggggcggt gggcgcttat caggcgggag tggttaaggc actggcagag tgtggtacac
      481 agatcagcat ggtttcaggg gccagcattg gcgcattcaa tggtgccatt atcgcggcct
      541 ctcccgatct gtcagaagct gccgtacgcc tggaggcgct ctgggatcat cttgggaata
      601 atcaggtgct gtcggtaaac cgatcggttt acttttcatt gctgaaaaaa ttgttccagg
      661 caatgaacct ctgccagatc cccggacgtg caggagcact gcttacgaca cttcttcgcc
      721 atatatcgat aatcaacggc ttcgacaatc cgatgactca gccgttgttg tcagatgggc
      781 ccctgacagc gttgatggat cattatcttg atacagatgc tctggcaggc gggctaccgc
      841 tgtatgtgtc gctgtacccc acagaagggg gcatgcagga tattattgac tgcattcgtg
      901 ctgaactggg ttccggaacc acgaaaaacg ccgtttttca gcatatccag agtctgcccc
      961 gcggacagca gaaagaggct ctgcttgcgt cagccgcact gcccctgttg ttccgtcccc
     1021 gtgaggttca ggggacaatg ttcggtgatg gtggtatggg aggatggcga aatatgcagg
     1081 gaaatacccc tgtaacgcct ctggtcgatg ccggatgcaa tatggtgatt gtgtcgcatc
     1141 tgagtgacgg ttctttatgg gatcgccagg cttttccgga caccacaatc cttgagatcc
     1201 gtccccggaa aaggctgaaa tatgcaggtg atggtggcaa cagtggcggt ctgctcagtt
     1261 tcacgtcggc acataccgac gcctggcgtc agcagggcta cgaggacacg atgctgacga
     1321 tggagcatat ccggaaaccg ctggca
//
LOCUS       DAJTYF010000285         1345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTYF010000285 DAJTYF010000000
VERSION     DAJTYF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.292"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            443..1192
                     /locus_tag="OO590_004272"
     CDS             443..1192
                     /locus_tag="OO590_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR6639368.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          376 a          274 c          279 g          416 t
ORIGIN      
        1 gagtggtgga ttagcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta
       61 cccgtaaata ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
      121 caggctacct caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga
      181 gtcattgtct gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaagaa
      241 cttaattcta actgtttgtt ctggatgttt taaacgtcct cctttttatg cttgttcata
      301 acggatattt aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat
      361 agcgcacaca attttattat ccagtacttt atactcttaa taaaataatt ataaatattt
      421 atcctctata aggagatgtt atatgaaaag acagcttttt actcttagta ttgtcggggt
      481 attttcttta aatacctttg catcattccc tccgggaaat gatgtgacaa caaaacctga
      541 cctttactac ctgacaaacg ataatgctat tgacagtctg gcattattac cgcctccacc
      601 acaaatcgga agtattgcct tcctgaatga ccaggccatg tatgaaaagg ggcgtttatt
      661 gcgaaatact gaaagaggga agctggcagc tgaggatgcc aatctcagta gtggtggcgt
      721 ggcaaatgta ttttctgctg cttttggttc tccgataacg gctaaagatt caccagaatt
      781 gcataaatta ctgacaaata tgattgagga tgccggagac ctggcaacgc gttcagcaaa
      841 agaatattat atgcgtattc gaccttttgc tttttatggt gtttctacgt gtaatacaaa
      901 agaacaggat acattatcca gaaatggctc ttatccatca ggtcatacat cgattggttg
      961 ggcaacagca cttgtattgt ccgaaataaa tccggcacgc caggatacca ttctgaaacg
     1021 gggttatgaa ctgggggaca gcagggttat ttgcggttat cactggcaaa gtgatgttga
     1081 cgcagcacgt attgttggct ctgctattgt agcaacactg cactcaaatc ctgtgttcca
     1141 ggcacaatta caaaaagcga aagatgaatt cgctaacaat cagaaaaaat aatgctttta
     1201 gccagtacgc cttataaccc cgaccttcag ggctaatgcc agtcagttaa gcaactgact
     1261 ggctcttttt cagggctgtg gggtatttcc agggcctctc ctttaccact ctcgggaagg
     1321 ccctttccct tcttgtcggt aattt
//
LOCUS       DAJTYF010000286         1331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTYF010000286 DAJTYF010000000
VERSION     DAJTYF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.293"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..534
                     /locus_tag="OO590_004273"
     CDS             1..534
                     /locus_tag="OO590_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6639369.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(563..1090)
                     /locus_tag="OO590_004274"
     CDS             complement(563..1090)
                     /locus_tag="OO590_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6639370.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1092..1331)
                     /locus_tag="OO590_004275"
                     /pseudo
     CDS             complement(1092..1331)
                     /locus_tag="OO590_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171265.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          382 a          304 c          279 g          366 t
ORIGIN      
        1 atgatgcact taaaaaacat ttctgctggt aatcctgaaa caaaagagca ataccagcta
       61 acgaaacaat ttaacatcaa atggctttat acagaggatg ggaaaaactg gtatgaggaa
      121 caaaagaact ttcagcctga tacgttgaaa atggtctatg accacaacgg cgttattatt
      181 tgtattgaaa aggatgtttc agcaattaat ccggaaggcg caagcgtcgt tgaggttcct
      241 gatattacag caaatcgccg ggctgatatt tcaggtaaat ggatgttcaa agatggcata
      301 gtgataaagc gaacttatac cggggaagag cagaggcaac aagtggaaaa tgaaaagcaa
      361 agcctgttgc aacttgtcag ggataaaacc cagctatggg actcacagct acgactgggc
      421 atcatttccg ccgggaataa gcagaaatta accgagtgga tgctctacgc gcagaaggtc
      481 gaatccacag acacctccag tctgccagta acgtttcccg aacaaccaga atgagagaag
      541 gcccgctatc gggccttaat ttttactctg gtttttgtgg ccattctggc tttgccgtat
      601 ccacacggct gaccagaaca ctgtagcgtt cccatgactc cagtcgtgcg cattcctcat
      661 ctgttgccat attcagcctg acagagcgct ccagcggctg gatgactgat tcagcttcgg
      721 aaagcaatgc ggctttttgt gattcggcct gttgctgctg ttcgtctgcc gtataaatcc
      781 gcttaaccac agctccgtcc ttaaacatcc acttcccgga atcatcggca cgacgatttg
      841 ctgttatatc ggggacctca acgacgctaa agccttcagg attaagcgtg gaggcatctt
      901 tagtgatggc gacaataata ttattttcat cgtaaacaat ctttattgta tctggctgaa
      961 agttcttcac ttccccatac cagttttttc cgtcttctgt ccacaaccag attacgtcaa
     1021 aattctttgt taattcgtac tgttccagtg ttttagcgtt acccgcttta atgttcttta
     1081 agtgcatcat attaaacgct cgctacatta taccaagtgc catttatata cttttgaatc
     1141 ggtctgtaat aaacacccgc aatattatcg gcagagttga acctgtatcc tgaacattaa
     1201 taccagacaa tacatgacct gaagggcact gaaaattcca tgtttgccag ttgttcactc
     1261 cataatattg ctgtgaacca agtcgaacat ctttcacata tctggaatca aaattgccat
     1321 agttgccggg a
//
LOCUS       DAJTYF010000287         1319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTYF010000287 DAJTYF010000000
VERSION     DAJTYF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.294"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(1..264)
                     /locus_tag="OO590_004276"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="OO590_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO590_004277"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO590_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6639371.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1170..1319)
                     /locus_tag="OO590_004278"
                     /pseudo
     CDS             complement(1170..1319)
                     /locus_tag="OO590_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          383 a          330 c          212 g          394 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg catctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag ggctaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg cataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccact
//
LOCUS       DAJTYF010000288         1301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTYF010000288 DAJTYF010000000
VERSION     DAJTYF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.295"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO590_004279"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO590_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6639372.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPIFTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          367 a          234 c          290 g          410 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta aaaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt agtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtcgtg agccgttttc agacaatcgc a
//
LOCUS       DAJTYF010000289         1272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTYF010000289 DAJTYF010000000
VERSION     DAJTYF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.296"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..486
                     /locus_tag="OO590_004280"
                     /pseudo
     CDS             1..486
                     /locus_tag="OO590_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343122.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            396..623
                     /locus_tag="OO590_004281"
     CDS             396..623
                     /locus_tag="OO590_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR6639373.1"
                     /translation="MLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYGLCANPACI
                     AYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            601..897
                     /locus_tag="OO590_004282"
     CDS             601..897
                     /locus_tag="OO590_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR6639374.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            903..>1272
                     /locus_tag="OO590_004283"
     CDS             903..>1272
                     /locus_tag="OO590_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6639375.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNR"
BASE COUNT          395 a          282 c          325 g          270 t
ORIGIN      
        1 ttgatgagaa atatcaggaa ctgcgggcag aactccatga agcccggaaa aacattccgc
       61 ccaaaaatcc tgtcgatgca gacaaattac tggctgcctc tcgcggagaa tttgttgaag
      121 ggattagcga cccgaatgat tcgaaatgga ttcccggtca tcatatctcc tcaaatgaag
      181 tcaaaaaaac ggaaaatgaa gtgcgacaga ctgaagataa acagcaccag aacagcgaac
      241 cggaggaggg aacaccctgc aatcagactg gcgaggataa ctgccctgac tgtggcgcgg
      301 tgatgggaga cgcaacttat caggaaactt ttgacgaaaa aaaaccagat gaagcacagg
      361 aagaagagcc gaagaaaacg gaaaaagctg atgatttgtt accggagaac gctggcagcg
      421 atcagcataa tgatagcaat aatgaaactg gcgaagatga actcaactgg aaaaaacagg
      481 tgttgattgc ggcggtttac ggtttatgtg cgaatcctgc gtgcatagcc tatgcgcctg
      541 caatccctga catcgcaatg atgattgcca acaagcttga aaattttgga gtgaccagtg
      601 atgagcgctt acccgatttt tgatcgtatt gaagaaatgg catgggctcg ccactaccag
      661 cagatcgttc gtgaagaaaa agaaacggaa ctggcggacg acctggaaaa aggtctgcct
      721 cagcacctgt ttgaatcgct ctgcatcgac catttgcaac gccacggtgc caataaacag
      781 gcaatcagcc acgcatttga tgatgatgtt gagtttcagg agcgcatggc agaacacatc
      841 cggtacatgg ctgaaaccat tgcccaccac caggttgata ttgatttgga ggactaaaat
      901 ctatggcgct ttttcaacga gcaataaaca cacaggccta tctaaaagca ggaattatgg
      961 ggtttgctgg cgacggcaaa acctacacag caagcgaact ggctattggt ctggtaatgc
     1021 ttatgcggca acggggaatt gaagctggta accgcccggt aatgtttctg gacacagaaa
     1081 caggctctga ctgggttaaa ccccgctttg atgcggaaaa tatcgaatta tttacggcaa
     1141 aaacccgctc gtttgttgat ctgcttgaag ccattaatga agcggaaagc agcggttccg
     1201 taatgatcat cgactccatc agtcacttct ggaccggatt atgcgacgag tatgccagac
     1261 gccgaaaccg ta
//
LOCUS       DAJTYF010000290         1266 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTYF010000290 DAJTYF010000000
VERSION     DAJTYF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.297"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..339
                     /locus_tag="OO590_004284"
     CDS             <1..339
                     /locus_tag="OO590_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639376.1"
                     /translation="DDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGV
                     QDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLI
                     NGTWYNVASI"
     gene            342..887
                     /locus_tag="OO590_004285"
     CDS             342..887
                     /locus_tag="OO590_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6639377.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            911..>1266
                     /locus_tag="OO590_004286"
     CDS             911..>1266
                     /locus_tag="OO590_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6639378.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGA"
BASE COUNT          357 a          273 c          343 g          293 t
ORIGIN      
        1 gatgattcag gctggcattt tttcagccag agaaacccgg ataacagcat agtgttcact
       61 gttaacggac aggtaattcc gttaaattac ggaaacttcg atgcccgcta taaatatcga
      121 acagaggggg tacaggatgt acggtatggc catgaaatgt attacagccc cggcagtaac
      181 accgtttcgt ggagattttg cgcaccttcg ggacacgggc tgtcagggat ggcgatatcg
      241 gataccggcc gtaactcagc ggataacgtt gacggtgtgt attaccgacc actgcaaaaa
      301 ctgattaatg gcacctggta taacgtagcg agtatttaac aatgttgcat ttaaaaaata
      361 ttactgcggg taatccgaaa acagcagaac aatatcagat gacaaaacga tattcggtca
      421 cctggctttt ttcagaagac ggaaaaaact ggtatgaaga gctgaagaat ttcgccagcg
      481 acacaataaa aatagcttac accggagatg gtcgcgtggt gtgggtcggt aaggatgtga
      541 caggcatcga gccacgcaat gccagcgtta tcgaagttcc ggatattacc gctaaccgac
      601 ggattaccgc gccgggttac tggttttacc gcaatgatga atttgtcttt gactacagac
      661 tcaaagcgga agatgagcgt gatgcccttc tggctcaggt cagtgcccgg acaggggaat
      721 gggaagaaga cctgctgctg gggttaatca gcgacgaaga taaagaaaag ctgaaagcct
      781 gtcgtattta cgcgaaatcg ctgcaggcga tggatttcag caccatcact gataaatcct
      841 catacaacgc cattgaatgg cccgcctctc cggaaggttc ttcctgattt aatttatcgc
      901 gagaaaaaca atgtctgtag tgatatcagg tgcgctgact gatggcgcag gtatccccat
      961 gtccggatac catattattc tgaaatcccg ggtaaacacc ccggaagtgg tgatgaacac
     1021 tgttgctgat gtgatgacag gaaacgatgg tgaatactgt ttccatgcgc ggactggaaa
     1081 atatggtgtg tatctgaaac aggactggcg caacgagtac aacgttggcg acattgctgt
     1141 atatgaggac tcaaagcccg gcacgctgaa tgactttctg attgctcctg atgagggcga
     1201 cctgaaaccg gatgtcgtca aacgctttga ggaaatggtg gcgcaggcgc agcagagcgc
     1261 cggggc
//
LOCUS       DAJTYF010000291         1226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTYF010000291 DAJTYF010000000
VERSION     DAJTYF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.298"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            134..625
                     /locus_tag="OO590_004287"
     CDS             134..625
                     /locus_tag="OO590_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639379.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            709..>1226
                     /locus_tag="OO590_004288"
     CDS             709..>1226
                     /locus_tag="OO590_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639380.1"
                     /translation="MNMTKNAFQFGIEPVHITDNDNLQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGAEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTV"
BASE COUNT          316 a          338 c          293 g          279 t
ORIGIN      
        1 cgcttaaccg ttagtagctc acctactcaa tacctaattt aaacaccttt tgagatgacc
       61 gatgcgggga gacccgtagg ccttactgtg cctgcgatac cgatagtctc aactctcaag
      121 aggagaatta cctatgatgt tcaacaacaa caactggaag ctgtcagtca ccgacatcaa
      181 cctttatgaa aacactgtga gtctggacgg tcagtcctat ccgttgtcgc tcgctatcaa
      241 gaccctcatt ccgggctacc tgtcaggctt accgtccacc agccgcgagg cgatggagtt
      301 actggaagct ctggctgaag ctggcgtgac cattggtaac ttcttcagta atgacctgat
      361 gaccgcctac caacgccgcc agatgaacaa acgagctgaa gctgagcgta tcgctaaaga
      421 gctagcgtct cagaaagagc gtacccgcga aatgttcatg accgaagacg aatggcaaaa
      481 agagctacag cgccgcgaac aggtcaaagc tgaacgccgg acctatggtg aaaacctccg
      541 cagcgctact cattccgctg gtcgctcacg tgccgctatc gtcgctgacc ttgagtcagg
      601 cggtaactgg atggacagct tgtaagcaag cgtccctcat ctgagaacac acggtccctg
      661 actgcctgtt agggacttca agtatttgac tcaaggagat taattataat gaatatgact
      721 aaaaacgctt tccagtttgg cattgagcct gttcatatca ccgacaacga caaccttcag
      781 gttaatgagg gccttccgac taacgccgac cctcaggtct atgccctcca gttagctaag
      841 actgttaagg ctatgctgaa cggtgtactt aaagacgctc aagataacat cccgttccct
      901 gtagaagtcc tgccgacccg taacagctta ccgactccaa tcattgctca cactctggct
      961 gaccgctctg tgctggtccc tgtccgtggt ggtaagcgac ctgaagttgt gaccgctccg
     1021 tctggcgctg aaatcaccgt tgagccgatt gaacaggcga tcctcgtgtc tcaccaaaca
     1081 aagctttggg accagaagtc gaccactggt ttcactcagg ggacgctcca gcaggacgca
     1141 ctgaacatct gcgataacgt gattcgtacc attaactcta agatggttga cgtgctggag
     1201 tcctcaaagc tgctgaagac cgtaga
//
LOCUS       DAJTYF010000292         1205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTYF010000292 DAJTYF010000000
VERSION     DAJTYF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.299"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            14..925
                     /locus_tag="OO590_004289"
     CDS             14..925
                     /locus_tag="OO590_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6639381.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            941..1168
                     /locus_tag="OO590_004290"
     CDS             941..1168
                     /locus_tag="OO590_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639382.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          338 a          256 c          336 g          275 t
ORIGIN      
        1 cactattaac gctttgatta acgagggcga caagtctcag gaagatattg ctgatgaagc
       61 cggaatgtct ccggcaacac tctcggctct gcgtaaagga gagtacaacg gtaatgtcga
      121 aggtatgcat gacaaattac agaaatggta tcaggtctgg cagaagggac agaacttgtc
      181 aggtacccct ggtgttgtga agacccagat attttgtgat ttgcatgagc tgtttaaaac
      241 agtgcgctca ctcgggatta tcagtgtcgt tgttggcgta ccgggtgtcg gaaaaacctt
      301 tgctgcccgg gaatattgtc gcaggacagc taatgcctgg atggtgactc ttgctccggc
      361 acattcgagc gtaacggaat gcctccttga aatagcatat gcgctggaaa ttaataatcc
      421 gggaagaaat aaggggaata taacccgcgc cattcgcaga aaactgggtg gtggtaaaga
      481 aaccagtggc cgtgttaacc cccttctgat tattgatgaa gctgatcact taagtgtgga
      541 tggtctggag caattacgcg ccattcagga tgccactggc gtggggatgg ttttaattgg
      601 caacccgaaa cagatggcgg atgccacccg tcgtgggact gatgaactgg cccgtctgtt
      661 cagccgtttt gccatgacaa aacaactgcg caaagtaaaa aagaccgatg tgatggctgt
      721 agccaaagca tggggagtaa cacaggaaga tgagctggac ctgctgatga gggtagccga
      781 aaagcccggg ggattgcgtg tattaactca cacactgaat catgcgtggc tggcggcaaa
      841 aggtgcaggc acatccctga ataaaagtca cattaaagcg gcttttaagg aggcttacag
      901 caaccccagg ttactgaact ggtagtaaag gacataaacc atgatgtcac gaaatataaa
      961 aatggcaacg gatgcgcaga actggttaca ggcgcacggg agtcacgtaa atgaatcata
     1021 tctcggcgtg gcgcgtccgg tacttgaaat cacctgccca ccaccggagc tggtcagaaa
     1081 cgctgtcagg attatggagc ataaatcagg ggttgcccgt tcagtatgga cggcccgcct
     1141 taatggctgc cagattatct ggcgttaata acggcttatt tatttgtgag gaataacaaa
     1201 atggc
//
LOCUS       DAJTYF010000293         1194 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTYF010000293 DAJTYF010000000
VERSION     DAJTYF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.300"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(224..496)
                     /locus_tag="OO590_004291"
     CDS             complement(224..496)
                     /locus_tag="OO590_004291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639383.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASGSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            585..764
                     /locus_tag="OO590_004292"
     CDS             585..764
                     /locus_tag="OO590_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639384.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGISAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            816..1010
                     /locus_tag="OO590_004293"
     CDS             816..1010
                     /locus_tag="OO590_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639385.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
BASE COUNT          391 a          277 c          248 g          278 t
ORIGIN      
        1 gaatgaaccg cgcctaaaat cagcaaggac acatggcaca aagtgcgaca tgtctcacat
       61 ttaaccccag tgggggcaac aagtataaaa ccgttttttg attaagtggt cgaatatgaa
      121 aaggggaagg tttatgtgaa aaatacctgt gttttctttg aaaacacaca acagtatcag
      181 gttatttatg tttatacagt gcgttccaaa ctgacctgaa taattacgca agttgcaaca
      241 ataaaacatt tttgccgtag tgtgaggtac tccggtaaat tgctttccag gtgcagacaa
      301 tgactaacga agaactacag gcaatcgcat tatccatgct tgagcgccag cgcgaaaaag
      361 tacaccagca acgagagtct ttgcttaacc catctccatt caccaggcca aaacgattgg
      421 agcctgaagc attcttggac aaatacccac ggcggcttaa atcaggcaaa accagaccac
      481 caggctgata gctcacagtt tgttctccga ttcaataaaa cggataagaa ccatcagcgc
      541 ggtagcgtaa ggcactacag cgcattgctt tacgagtaat aacaatggat ttatcaccag
      601 aagacgcaaa gtttttacga gaactgcaac accgtatgct cagagaagcc attatcgaac
      661 agcaaacggg cataagcgcc atagacccat tgcgatacaa acgggcctca tctcctttag
      721 ttgacgacaa ttccaatgcg gataaaacac gtccaccaca ctgattacat ttttcattag
      781 cacagtggtg tgatgcagca tacgaggaaa accggatgaa tgagaacaaa ataaaaaggc
      841 tggagcaact gttacaggca cgccaacggg agtttgccac taaatgggga cgtgccgcgc
      901 tgccacatga acgcctaacc tattttccac ttgaagacct cattaaaaat aatggcaaaa
      961 aaatcaggtc tgataagaaa cacttcttag aatgcaatga agacaaataa caacaatcta
     1021 catattgcca ccaaccacag atgtggcatc gttatcagcg cacttgattt tctttatatc
     1081 cactgacgag cttgccgggg cacctttgtt tgccggtcat tctggtatca tgcagatatt
     1141 tacggcgtac aagcccacta gcatgacatt gtaataaggc ctgagcctaa cgct
//
LOCUS       DAJTYF010000294         1179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTYF010000294 DAJTYF010000000
VERSION     DAJTYF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.301"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..708)
                     /locus_tag="OO590_004294"
     CDS             complement(<1..708)
                     /locus_tag="OO590_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6639386.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLN"
     gene            800..886
                     /locus_tag="OO590_004295"
                     /pseudo
     CDS             800..886
                     /locus_tag="OO590_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          328 a          200 c          308 g          343 t
ORIGIN      
        1 gttcagctga ttgttaggga tatatagtga ttgcagacgt tgtggaagtt cgggcagtct
       61 gtgtagttgg ttttccccaa cgtttaaaag cctcagattt tgaggaaggg gcggtagctc
      121 ttgtaatctg ttatcaaaag cacttagttc ctgtagttca catggtaatt ctggtaaggc
      181 agtaagctgg ttgttgccaa cagaaagttt ttgtagagcc gggggaagat caggtagcgt
      241 ctccaggcta ttgagaaggg ctgagagtga ctgcaaggaa gatggaagag aaggcaaaca
      301 tgttaaaact ctgttaagtg aaacatcaag agcgactagg tgaggcggaa gagccgggag
      361 cctgctcagt ctgttatcgc tggctttaag tacagttaag gacggaggga gttctggcag
      421 agagcgtagc tcattgctag agatgttaag ttcttgtatg tgcgggggca ggtatgggag
      481 agagcgtaat ctgagtaaac ttaaattgag ggctggctct tgaaaagcca gacatatttt
      541 cagtaatcga actgcctgtg ttcggtcttc tgttgcagca ccttccttgg cccagttatc
      601 ccagatgcgg tgataatccg tgatattttg ctcttctacg gataaacggg atatacatcc
      661 taaaccacaa aaggagcgga aaaaagaagg attgtggaca ggtttcattt attattgatc
      721 ctcattgttt atatattaat atattcgtgc aaatggagtg aggattcttt aaaaaaacgc
      781 gcctgtaaat agttaagaac aggaagaact gttggggatg tggctggccg aaaatgaagg
      841 ggtgaagttc tggctcaatg tgctgactga actgaaaaaa cgcggttggt cgccctacct
      901 tttcatcaag ccagtagtct gcccaccaca gcatcatttc tctgcgctta tcgagatact
      961 gagcatggtt gtaaattcag ctaacccagt gcgttgacaa aaaattagcg caagaggaca
     1021 aaaaatcacc ttgcgctaat gctctgtatc aggtcactaa taccatctaa gtggttgatt
     1081 catagtgact ggatatgttg tgttttgcag tattgtgcag tctatttttt aagctaaatg
     1141 tattataatg cattgatatt aatgattttt actgtttct
//
LOCUS       DAJTYF010000295         1155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTYF010000295 DAJTYF010000000
VERSION     DAJTYF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.302"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..1155
                     /locus_tag="OO590_004296"
                     /pseudo
     CDS             1..1155
                     /locus_tag="OO590_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
BASE COUNT          224 a          312 c          302 g          317 t
ORIGIN      
        1 catgaccatg ttcaagatgc cagtatttac ctaggcatca ctgtgcacga acgtactgat
       61 tattgcttcc ttcccaattc tgacggttac cgtcgcgttg ttgaccctgg atcgctatct
      121 gggcacccat ttctttacca acgatatggg tggcaacatg atgatgtaca tcaacctgat
      181 ttgggcctgg ggccacccgg aagtttacat cctgatcctg cctgttttcg gtgtgttctc
      241 cgaaattgcg gcaaccttct cgcgtaaacg tctgtttggt tatacctcgc tggtatgggc
      301 aaccgtctgc atcaccgtgc tgtcgttcat cgtttggctg caccacttct ttacgatggg
      361 ggcgggcgcg aacgtaaacg ccttctttgg tatcaccaca atgattatcg ccatcccgac
      421 cggggtgaag atcttcaact ggctgttcac catgtatcag ggccgcatcg tgttccattc
      481 tgcgatgctg tggactatcg gttttatcgt caccttctcg gtgggcggta tgactggcgt
      541 gctgctggca gtaccgggcg cggacttcgt tctgcataac agcctgttcc tgattgcgca
      601 cttccataac gtgatcatcg gcggcgtggt cttcggctgc ttcgcaggga tgacctactg
      661 gtggcctaaa gcgttcggtt tcaaactgaa cgaaacctgg ggtaaacgcg cgttctggtt
      721 ttggatcatc ggcttcttcg ttgcctttat gccgctgtat gcgctgggct tcatgggcat
      781 gacccgtcgt ttgagccagc agattgaccc gcagttccac accatgctga tgattgcagc
      841 cagcggtgca gtactgattg cgctgggtat tctctgcctc gttattcaga tgtacgtttc
      901 tattcgcgac cgcgaccgcg accagaaccg tgacctgact ggcgacccgt ggggtggccg
      961 tacgctggag tgggcaacct cttccccgcc tccgttctat aactttgccg tagtgccgca
     1021 cgttcacgaa cgtgatgcat tctgggaaat gaaagagaaa ggcgaagcgt ataaaaagcc
     1081 tgaccactat gaagaaattc atatgccgaa aaacagcggt gcaggtatcg tcattgcagc
     1141 tttctccacc atctt
//
LOCUS       DAJTYF010000296         1133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTYF010000296 DAJTYF010000000
VERSION     DAJTYF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.303"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..89
                     /locus_tag="OO590_004297"
     CDS             <1..89
                     /locus_tag="OO590_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639387.1"
                     /translation="LASNGSTLTIQSNGAAFTATTIKIIAEQ"
     gene            89..544
                     /locus_tag="OO590_004298"
     CDS             89..544
                     /locus_tag="OO590_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6639388.1"
                     /translation="MIQFKPTRNIDLIEAVGNHTDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            547..>1133
                     /locus_tag="OO590_004299"
     CDS             547..>1133
                     /locus_tag="OO590_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6639389.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGT"
BASE COUNT          320 a          284 c          288 g          241 t
ORIGIN      
        1 ttcttgcctc aaatggcagt acgttaacta ttcagtcaaa tggtgcggcg tttactgcca
       61 caaccatcaa gataatcgcg gagcagtgat gattcagttc aaaccaacgc gaaacatcga
      121 cctgatcgaa gcagtaggaa atcacactga cattatcgcc gggagcaaca acggcgatgg
      181 atacgactac aagcctgaat gccgttactt tgaggttaac gtgcacgggc agttcggcgg
      241 aattgtttac tatcaggaga ttcagccgct aacctttgat tgccacgcca tgtacctgcc
      301 agagattcgt ggattcagca aggaaatcgg gctggcgttc tggcgataca ttctgactaa
      361 caccaccgtt cagtgcgtca catcgttcgc tgcgcgcaaa ttccgccacg gtcagatgta
      421 ctgcgcaatg attggcctta agcgtgtagg aaccatcaag aaatacttca aaggcgtgga
      481 tgacgtgacg ttttacagcg ccacacgcga agaactaatc gacttcctga atcacgggag
      541 atagccatgt tatatgcatt taagctgggc agaaaactgc gcggcgagga accttattgc
      601 cctgaaaaag gcgggaaagg tggcagctct gataaaagcg caaagtatgc agcagaagct
      661 cagaagtatg ccgcagacct gcaaaatcag cagtggcaga cgatcatgaa aaaccttgct
      721 ccgttcacgc ctcttgcgga gcagtatgtt aaccagcttc agaacctttc cagtttagaa
      781 ggtcaggggc aggcacttaa tcagtattac aactctcagc agtataaaga ccttgcaggt
      841 caggctcgtt accagagtct tgctgctgcg gaggcgacgg gtggacttgg ttcgacagcc
      901 acaagcaatc aactggctac gatcgcgccg acactcggtc agtcttggtt atcaaaccag
      961 atgagcaatt acaacaatct ggcaaacgtt gggcttggtg cgctgcaagg tcaggcaaac
     1021 gccgggcaga catacgccaa caacatgagc agcattgcac agcaaagcgc agctcttgcc
     1081 gctgctaatg ccaacaaacc atcaagtctt cagacagcaa ttagtggcgg aac
//
LOCUS       DAJTYF010000297         1114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTYF010000297 DAJTYF010000000
VERSION     DAJTYF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.304"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            5..>1114
                     /locus_tag="OO590_004300"
     CDS             join(5..266,266..>1114)
                     /locus_tag="OO590_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HCR6639390.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRH"
BASE COUNT          282 a          290 c          312 g          230 t
ORIGIN      
        1 tcacatgaca aaaacagtat caaccagtaa aaaaccccgt aaacagcatt cgcctgaatt
       61 tcgcagtgaa gccctgaagc ttgctgaacg catcggtgtt actgccgcag cccgtgaact
      121 cagcctgtat gaatcacagc tctacaactg gcgcagtaaa cagcaaaatc agcagacgtc
      181 ttctgaacgt gaactggaga tgtctaccga gattgcacgt ctcaaacgcc agctggcaga
      241 acgggatgaa gagctggcta tcctccaaaa ggccgcgaca tacttcgcga agcgcctgaa
      301 atgaagtatg tctttattga aaaacatcag gctgagttca gcatcaaagc aatgtgccgc
      361 gtgctccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac aaggataagc
      421 acgcgtcagc agttccgcca acactgcgac agcgttgtcc tcgcggcttt tacccggtca
      481 aaacagcgtt acggtgcccc acgcctgacg gatgaactgc gtgctcaggg ttaccccttt
      541 aacgtaaaaa ccgtggcggc aagcctgcgc cgtcagggac tgagggcaaa ggcctcccgg
      601 aagttcagcc cggtcagcta ccgcgcacac ggcctgcctg tgtcagaaaa tctgttggag
      661 caggattttt acgccagtgg cccgaaccag aagtggccag gagacatcac gtacttacgt
      721 acagatgaag gctggctgta tctggcagtg gtcattgacc tgtggtcacg tgccgttatt
      781 ggctggtcaa tgtcgccacg catgacggcg caactggcct gcgatgccct gcagatggcg
      841 ctgtggcggc gtaagaggcc ccggaacgtt atcgttcaca cggaccgtgg aggccagtac
      901 tgttcagcag attatcaggc gcaactgaaa cggcataatc tgcgtggaag tatgagcgca
      961 aaaggttgct gctacgataa tgcctgcgtg gaaagcttct ttcattcgct gaaagtggaa
     1021 tgtatccatg gagaacactt tatcagccgg gaaataatgc gggcaacggt gtttaattat
     1081 atcgaatgtg attacaatcg gtggcggcgg caca
//
LOCUS       DAJTYF010000298         1107 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTYF010000298 DAJTYF010000000
VERSION     DAJTYF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.305"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..387)
                     /locus_tag="OO590_004301"
     CDS             complement(<1..387)
                     /locus_tag="OO590_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR6639391.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQVDLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNV"
     gene            816..1097
                     /locus_tag="OO590_004302"
     CDS             816..1097
                     /locus_tag="OO590_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR6639392.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          308 a          250 c          251 g          298 t
ORIGIN      
        1 aacgttaggt tcacgcttat aatcggccgg cgcaatcgta tagccgttta caggcgctat
       61 cagcgatgag cataaaaaat ggttatcacg ggcatagatc aattcattaa tataaagata
      121 gccacgaata atattcaaca ttcgcttttg atgggctgga gtgcaaactt gcccctgata
      181 gcgttcagct gcatcgctca ctaaatctac ctggcgaatg accagctcgg atttatccag
      241 tgccagttga gcaaaggttc ggagctgatt attcacttct gattttgccc aaaggaaggc
      301 aatccatagc gaaagaatga tgggaaagag aactaaaaag ataatgcccg gcaacgccag
      361 taattggtgt cgtgcacgat gactcattgc tatcaatttc ctgcctgttt atacccggcc
      421 attgcaaggt gaaaccctgg ctaccgacgt ctttagataa atcttacggt ataaatagca
      481 cttttgcctg atttgaaatt gatacctttt taatcgtcac ctggatgaaa ataattcatt
      541 gatattcaaa attaattttc tacaggaata cagaggcggc gggaagatat gcccttaatg
      601 ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa
      661 acaatcaatt gtgatatggt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt
      721 gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg
      781 tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg
      841 ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc acactcaaca
      901 tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg
      961 tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac atcaccgcta
     1021 tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc
     1081 caatgatttt gacctgatac gtagaag
//
LOCUS       DAJTYF010000299         1100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTYF010000299 DAJTYF010000000
VERSION     DAJTYF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.306"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..1019
                     /locus_tag="OO590_004303"
                     /pseudo
     CDS             1..1019
                     /locus_tag="OO590_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          269 c          253 g          266 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgagaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga gattccacaa acccagcaag
      841 gggacattgg cgcgaaagat attccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc
//
LOCUS       DAJTYF010000300         1085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTYF010000300 DAJTYF010000000
VERSION     DAJTYF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.307"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..685)
                     /locus_tag="OO590_004304"
     CDS             complement(<1..685)
                     /locus_tag="OO590_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6639393.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHY"
     gene            complement(685..1050)
                     /locus_tag="OO590_004305"
     CDS             complement(685..1050)
                     /locus_tag="OO590_004305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639394.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVAPPF"
BASE COUNT          253 a          311 c          275 g          246 t
ORIGIN      
        1 ggtaatgcgt gtccaggaag cgattccatt cccgtttact gcttccttct gctgccagcg
       61 catccggcat caccaggtca gggtatttcc gtgacagcaa ccgtgttatc cggtagcgcc
      121 acatgctcat caccttacgg gcgtaaaaat gaagattttt ccaggtgtgg cccgacgtca
      181 caccaccggc agttgtcgat aaatggatat gcggatgcca ctgctggtca cgcccccatg
      241 tgtggatcac cgtgaatatc cccggctcca catctgcctg atggcagatt tccagtatca
      301 catccgctgc aatgcggctc atctctgcca gtaaccaccg gttgtggaac accagggacc
      361 agtactggca gggaagtgtg aacacaatat gctgccacgg gcagtcgggg accaggctca
      421 gcagatactg tatccactgt gcgccagcct tcaccccgca gtgcgggcag gagcggcttt
      481 tacaccggaa gcagaccttt tttgtatggc aacagtccgg tgatgaacaa caccactgcg
      541 tgtagcccat cagtgtggtc ccgcaggcca tgatttttgt cactgattca attaccaccg
      601 gacggactgc cccttccggt tgtttctcca gccagttaag ccagcggttt ccctgctgaa
      661 aaatatcggc aaaacgggga agcatcagaa gggcggggcg actccgtccg gccagtgaac
      721 cgtgccacac tccgggcagt acataccgcc ggcgctgata ccggcaagaa tggtcgcaaa
      781 ttcccgctcc gtgcagcggg cgatttccgg atacccttcg tcatcaacac gtacaaacca
      841 gaagaccagc tttttgtttc ccgcatccac aaagaacgga atattcaggt ctgcgcagca
      901 ttcaacggca tcgtcaaaac tatcaaagcg cagaacttct gcgtcttctt cgtcaaaaaa
      961 atcatcttcg tgaagcttca cgacatagcg gggaagtttg cttctttgag aggcgggttt
     1021 acgtttacgg ggtttagctg aacgggccat ataaccacct gaaagacaat gacattgcct
     1081 gtttt
//
LOCUS       DAJTYF010000301         1085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTYF010000301 DAJTYF010000000
VERSION     DAJTYF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.308"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..476
                     /gene="ybcL"
                     /locus_tag="OO590_004306"
     CDS             <1..476
                     /gene="ybcL"
                     /locus_tag="OO590_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6639395.1"
                     /translation="NEIKTGEQLTTSHVFSGFGCEGGNTSPSLTWSGAPEGTKSFAVT
                     VYDPDAPTGSGWWHWTVANIPATVTYLPTDAGRRDGTKLPTGAVQGRNDFGYAGFGGA
                     CPPKGDKPHHYQFKVWALKTDKIPVDSNSSGALVGYMLNANKIATAEITPVYEIK"
     gene            486..>1085
                     /locus_tag="OO590_004307"
     CDS             486..>1085
                     /locus_tag="OO590_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6639396.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
BASE COUNT          341 a          203 c          222 g          319 t
ORIGIN      
        1 gtaatgaaat aaaaacagga gagcaactta caacgtctca tgtattttct ggatttgggt
       61 gtgaaggtgg taatacatcg ccctcattaa cctggtctgg tgctcctgaa ggcaccaaaa
      121 gctttgccgt aactgtgtat gatccagatg ctcctacagg cagtggatgg tggcactgga
      181 ctgttgctaa tattccagca actgtaacat atttgcccac tgatgcagga agacgtgatg
      241 gtacaaaact tccgactggt gctgttcaag gccgaaatga ttttggttat gcaggatttg
      301 gtggagcatg tcctcccaaa ggagataaac cccatcatta ccagtttaaa gtatgggctc
      361 taaaaactga taagattcct gtggattcta attccagcgg agcgttagtt ggttatatgc
      421 ttaatgccaa taaaattgca accgctgaga taacaccagt ttatgagata aagtagggta
      481 agagtatgct ggcaaaagat aagtctaacc taaagattga agaaatacgg atgcataaac
      541 atcatgagat tcacagggtt aagcctctta tgccagcttt atgtcgtatc cgtcagggaa
      601 aaaaagttat caattgggag acgcatagtc taactgttga taataatcag ataatattat
      661 ttccctgtgg atatgaattt tatattgtga attatccaga agcaggtctt tatcttgcag
      721 aaatgcttta ttatcccatt gatctaattg aaaagtttca aaaattttat gcgataactg
      781 atcaaattcg taacacgaca ggtttctgct tacctcagaa ccccgagtta atatattgtt
      841 gggagcaact aaaaacatct atttcccgag gcttctcaac gcaaattcag gaacacttag
      901 caatgggcgt tctactttca ttaggagcac atcatgttaa ttgtttactt ttatcagata
      961 gtaaacaatc attaataagt cgttgttata acctaatgct atccgaacct agaacaaaat
     1021 ggacagcaaa caaggtagcg agatatctct acatttctgt ttccacattg catcgccgtc
     1081 tggca
//
LOCUS       DAJTYF010000302         1076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTYF010000302 DAJTYF010000000
VERSION     DAJTYF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.309"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..99)
                     /locus_tag="OO590_004308"
     CDS             complement(<1..99)
                     /locus_tag="OO590_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6639397.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLY"
     gene            complement(171..1055)
                     /locus_tag="OO590_004309"
     CDS             complement(171..1055)
                     /locus_tag="OO590_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCR6639398.1"
                     /translation="MVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDD
                     PFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETT
                     DLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLL
                     RLCLRQALQLADVVKISEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVC
                     YRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGAL
                     AVTAKGAMTALPCRQELE"
BASE COUNT          286 a          311 c          225 g          254 t
ORIGIN      
        1 gtataacgac caccacagcg accaggaaat aaaaaagaga aatgagtaac tggatgcaaa
       61 acgatagtac gcatttctga atggaatatt cagtgccata attacctgcc tgtcgttaaa
      121 aaattcacgt cctatttaga gataagagcg acttcgccgt ttacttctca ctattccagt
      181 tcttgtcgac atggcagcgc tgtcattgcc cctttcgccg ttactgcaag cgctccgcaa
      241 cgttgagcga gatcgataat tcgtcgcatt tctctctcat ctgtagataa tcccgtagag
      301 gacagacctg tgagtaaccc ggcaacgaac gcatctcccg cccccgtgct atcgacacaa
      361 ttcacagaca ttccagcaaa atggtgaact tgtcctcgat aacagaccac caccccttct
      421 gcacctttag tcaccaacag catggcgatc tcatactctt ttgccagggc gcatatatcc
      481 tgatcgttct gtgtttttcc actgataagt cgccattctt cttccgagat cttgacgaca
      541 tccgccagtt gtagcgcctg ccgcaaacac aagcggagca aatgctcgtc ttgccataga
      601 tcttcacgaa tattaggatc gaagctgaca aaacctccgg catgccggat cgccgtcatc
      661 gcagtaaatg cgctggtacg cgaaggctcg gcagacaacg caattgaaca gagatgtaac
      721 cattcgccat gtcgccagca gggcaagtct gtcgtctcta aaaaaagatc ggcactgggg
      781 cggaccataa acgtaaatga acgttcccct tgatcgttca gatcgacaag caccgtggat
      841 gtccggtgcc attcatcttg cttcagatac gtgatatcga ctccctcagt tagcagcgtt
      901 ctttgcatta acgccccaaa aggatcatca ccgacccgac ctataaaccc acttattccg
      961 cctaatctgg cgattcccac cgcaacgtta gctggcgcgc cgccaggaca aggcagtagg
     1021 cgcccgtctg attctggcaa gagatctacg accacatccc ctaaaaccca tacttt
//
LOCUS       DAJTYF010000303         1067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTYF010000303 DAJTYF010000000
VERSION     DAJTYF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.310"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..680)
                     /locus_tag="OO590_004310"
     CDS             complement(<1..680)
                     /locus_tag="OO590_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6639399.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLT"
     gene            777..>1067
                     /locus_tag="OO590_004311"
     CDS             777..>1067
                     /locus_tag="OO590_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HCR6639400.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSK"
BASE COUNT          294 a          210 c          250 g          313 t
ORIGIN      
        1 ttggtcaacg aatatccatc atcaagaagc gtcactgcag aaataacgtg catttgctga
       61 acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag tgtacggcta
      121 ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg gttgtttagc
      181 aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc aagtgacatt
      241 ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa acgtaactgt
      301 tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag taattccctt
      361 aacaggggtg aaatacccgt catgaagcaa actgttccca gcgtttcact ttccgcttct
      421 gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg cggcatttgt
      481 ggcggcagcc aaagcgcgct gctggcatgg actagccaga cgctttctcc agcccataca
      541 cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg ccagggaact
      601 gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg tgatgctttg
      661 ggttgcagtg taattgtcat ttacagtagg tattttgtca gaaatcatca aataagccat
      721 gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa agagtgatga
      781 taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg actggcgttg
      841 tcttttttat ctggagggta agcaaaggaa gatttataca gacgacggat gatgcttaca
      901 ttggtggtaa tatcaccacg gtggcaagta aagtttctgg ttatatttct gctatagagg
      961 tgcgcgataa tcagagcgta aaaaaaggcg atattatcct gcgtctcgat gatcgggatt
     1021 atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag tagcaaa
//
LOCUS       DAJTYF010000304         1050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTYF010000304 DAJTYF010000000
VERSION     DAJTYF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.311"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..770)
                     /gene="mppA"
                     /locus_tag="OO590_004312"
     CDS             complement(<1..770)
                     /gene="mppA"
                     /locus_tag="OO590_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6639401.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKR"
BASE COUNT          289 a          261 c          225 g          275 t
ORIGIN      
        1 taacgcttag tggctgcgga ttcctgatta attggcagga aggtcacttt ttgcagcacc
       61 gttttggcgt tatcccaata atgggtattc ggtaccacga ccagtttttc attgactacg
      121 cgctctttaa gaacataagc gccattgccg atcagatttc cgggtttcgt ccactcttta
      181 ccgctttcta cgttggcttt ttgcaccggg aagaaggcaa agttagcggt taaattcaca
      241 aaccacggca acggtttatc aagctgaatt ttcaaagtat gggcatcaac tgcggtgacg
      301 ccaagctgat caggcgtagc tttaccatca ataatcgcct gtgcgttgtt gattcccgcc
      361 agcgcggcaa accatgcaaa tggcgacaat gtttttgggt ccaccagacg ttgccagctg
      421 tagacaaaat cttgcgccgt taccggtgtg ccatccgccc attttgcgtt atcgcgcagg
      481 gtaaaagtcc agatacggtt gtcattactt ttccattgag tcgcgacgcc ggggacaatc
      541 tccccttttt cgttctgatt caccagacct tcaaacagat cgcgaatgac ctgaatctct
      601 ggcaggccca cggctttagc gggatccagc gacgcaggct catctttaat gtggcgcacc
      661 agctcctgct tctctgccag tactgtgccg ctcggaactt ctgcagcata cgaaagagaa
      721 atgctgctga ccaacagcgc acaacacgta actgaaacag agtgcttcat aagtttccct
      781 ctaaccgaca ttaaaattga tgcgtaatta tttgtttcgc cttaaagaaa tgcaaacaac
      841 ttccatcaaa aaagtgacaa aggaagcatc aaatgtttta agtatcaata tatcaaatca
      901 gatttagtgt aaataataga cgccaaaatg cggcaaagca catagaacac cgaacactac
      961 gttcgggctt cgcacgttca aaccagtttt cggtatcata atcagcatct gtcgccattg
     1021 tacgaagaat aaaaaataac caaaacgttg
//
LOCUS       DAJTYF010000305         1033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTYF010000305 DAJTYF010000000
VERSION     DAJTYF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.312"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO590_004313"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO590_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR6639402.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGSKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
BASE COUNT          241 a          227 c          265 g          300 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa tgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggcagtaaaa accgctttac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc tta
//
LOCUS       DAJTYF010000306         1030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTYF010000306 DAJTYF010000000
VERSION     DAJTYF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.313"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            373..>1030
                     /locus_tag="OO590_004314"
     CDS             373..>1030
                     /locus_tag="OO590_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6639403.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     M"
BASE COUNT          357 a          202 c          175 g          296 t
ORIGIN      
        1 gtttaagctc cattttcaaa aaaatatatc agggtttgtt actaataact tctgaaaaca
       61 gaaaaaaata gagcggtaat tctatttcgg agaggagtaa ctccatctat cctcgatacg
      121 tactgaacgt tatcgtgata tattcatcat gctttcttca ccattattcg agtataggga
      181 gagacgcaat gaccttaaat acctttttat ggctgaatta ccattctcag gaataacagc
      241 agactccttc ccatagacgg cttaggggga aagcaatacc cggagaaagg gtacaaagca
      301 aactgtttta aaattaataa gccatattct atttaaccag aattcttatc gttgataaga
      361 attctggtta taatgattaa atcaaccaat atacaggcaa tcggttctgg cattatgcat
      421 caaataaaca atatatactc gttaactcca ttatctttac ctatggaact gactccatct
      481 tgtaatgaat tttatttaaa agcctggagc gaatgggaaa agaacggtac cccaggcgag
      541 caacgcaata tcgccttcaa taggctgaaa atatgtttac aaaatcaaga ggcagaatta
      601 aatttatctg agttagattt aaaaacatta ccagatttac cgcctcagat aacaacgctg
      661 gaaataagaa aaaacctatt aacacatctc cctgatttac caccaatgct taaggtaata
      721 catgctcaat ttaatcaact ggaaagctta cctgccttac ccgagacgtt agaagagctt
      781 aatgcgggtg ataacaagat aaaagaatta ccatttcttc ctgaaaatct aactcattta
      841 cgggttcata ataaccgatt gcatattctg ccactattgc caccggaact aaaattactg
      901 gtagtttctg gaaacagatt agacagcatt cccccctttc cagataagct tgaagggctg
      961 gctctggcta ataattttat agaacaacta ccggaattac cttttagtat gaacagggct
     1021 gtgctaatga
//
LOCUS       DAJTYF010000307         1004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTYF010000307 DAJTYF010000000
VERSION     DAJTYF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.314"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..534
                     /locus_tag="OO590_004315"
                     /pseudo
     CDS             1..534
                     /locus_tag="OO590_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          310 a          223 c          209 g          262 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggcg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggattaact acataacaag gcgcaagata tatatccaaa aacaaataat
      721 accgttgcaa taattccttt cagtaaagga ggtgtgatta atttcagggg atttcagagt
      781 aaaaattgta caacatatta aaatatgttc atttcgttat aatacacaca agaaaatatt
      841 aagttagata atatattcta aataaccata gataaaaacg cacataatgg ggaatatttt
      901 atgaaaatac ctgaagcagt aaatcatatt aatgttcaga ataatattga tcttgttgat
      961 ggaaaaacga atccgaataa agcaacaaaa gcattacaaa aaat
//
LOCUS       DAJTYF010000308          986 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTYF010000308 DAJTYF010000000
VERSION     DAJTYF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.315"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>986
                     /gene="pdeF"
                     /locus_tag="OO590_004316"
     CDS             <1..>986
                     /gene="pdeF"
                     /locus_tag="OO590_004316"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6639404.1"
                     /translation="LALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFI
                     IIIILCWGGYRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLAS
                     RENLVGVMPFNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDA
                     KVTKKEFALWLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLIS
                     LLWAVVLMISIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRR
                     IQRLAYVDPVVHLPNVRALNRALRDALWSALCYLRIPGMEMLVKNYGIMLRI"
BASE COUNT          218 a          221 c          229 g          318 t
ORIGIN      
        1 ttggcactga tgatcagcat gatgatgttt ttcagctggg cggcattgcc agggatcacc
       61 ttagggattt ttgtccgcaa atatgcagag ctgggttttt acgaaacgct ctcattaacg
      121 gctaatttta ttatcattat cattctctgt tggggcggtt acagggtctt tactccccgg
      181 cgtaacaacg tttcacatgg tgatacccgt ttaatttccc agcgtatatt ctggcagata
      241 gtgtttcctg caacgctgtt tctgatactt ttccagtttg ctgcatttgt aggattactg
      301 gcgagcagag aaaatctggt cggtgtcatg ccttttaacc tcgggacttt aatcaattat
      361 caggccttac tggtgggtaa tctgatcggt gtcccgctgt gctacttcat cattcgggta
      421 gtgcgaaatc cattttattt acgtagctat tattcgcaat taaaacagca ggttgatgcc
      481 aaagtcacca aaaaagagtt cgcgctctgg ctactggcat taggtgcttt attgttgctg
      541 ttatgcatgc cgttaaatga aaaaagcaca atttttagca ccaattatac cttgtcatta
      601 ttgctgcccc tgatgatgtg gggagcaatg cgctatggtt ataagctgat ttcgctgctc
      661 tgggcggtcg tgttgatgat cagcatccac agctatcaaa attacattcc catttatcct
      721 ggctatacca cgcagctgac cataacctcc tccagttatc tggtattctc ttttattgtc
      781 aattatatgg ctgtactggc aactcgtcag cgagcggtag tcagacgcat tcagcggctt
      841 gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg ccctgaatcg cgcgttgcgt
      901 gatgccctct ggtctgcgct ttgttattta cgcatccctg gcatggaaat gctggttaag
      961 aactatggca tcatgctgcg gattca
//
LOCUS       DAJTYF010000309          985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTYF010000309 DAJTYF010000000
VERSION     DAJTYF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.316"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            496..>985
                     /locus_tag="OO590_004317"
     CDS             496..>985
                     /locus_tag="OO590_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR6639405.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLL"
BASE COUNT          277 a          180 c          239 g          289 t
ORIGIN      
        1 aggtaaagtc gcaaccccaa atcgtaaagg aaaccgtagc acgtcgtatg caagaacgtg
       61 ccacggctgg ctgatggatg ttcgatagcg cgagtttgaa tgaaaatcag ccggagatga
      121 ttttacataa ttgctacgga attattcaat acaggaattg cttgcgtctg catggattga
      181 cctgaaatat tcccgaaaat ttctctaaaa aactcgaaaa aaatggtaac tggttgaatg
      241 tattaatatg caatggtacg tgtcagggat taaaagatga acgtaaattt attcaacgca
      301 ttaattttaa agggttttat tgtttgttga cgaaaacagg aatcatgttc ggtctctttt
      361 tatctgttaa aagccagaag catttccttc gctgacttta tagtcaacca taacacacac
      421 tctactgtct gagtccagcg ttttttaaca ttcttgttaa gattatgtga tctttagcgc
      481 gggaggaaaa tattgatgaa acagcctgcg cccgtttatc agagaattgc gggtcatcaa
      541 tggcgacata tctggctttc tggcgatata cacggttgtc ttgagcagtt gcgccgcaaa
      601 ttatggcatt gtcgttttga tccgtggcga gatttactta tctcagtggg agacgttatc
      661 gatcgtgggc cgcaaagttt acgttgtctg cagttactgg aacaacattg ggttcgtgcg
      721 gtaagaggca atcatgaaca gatggcgatg gatgcgctgg cattccagca gatgtctttg
      781 tggttgatga atggcggcga ctggtttatt gcgctggcag ataatcaaca gaaacaagcg
      841 aaaacggcgc tggaaaaatg tcagcatttg ccctttattc ttgaagtaca cagtcgcacc
      901 ggcaagcatg ttattgctca tgccgattat ccagatgatg tttatgaatg gcaaaaggac
      961 gttgatttgc atcaggtctt gttga
//
LOCUS       DAJTYF010000310          973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTYF010000310 DAJTYF010000000
VERSION     DAJTYF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.317"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(10..813)
                     /gene="dkgB"
                     /locus_tag="OO590_004318"
     CDS             complement(10..813)
                     /gene="dkgB"
                     /locus_tag="OO590_004318"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6639406.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLHTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          257 a          218 c          216 g          282 t
ORIGIN      
        1 cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc ggtcgttgca
       61 atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt gtgccttaag
      121 attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac cttcccccat
      181 agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa tacgagcaat
      241 aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga tatgaatacc
      301 gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga gttcaatctg
      361 gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca atgggatcgt
      421 gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca gcagcgcctt
      481 cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta gcgtcagatc
      541 aacataatcg gtatgcaatt tttgcaggct ctctttcaga ctcgggatca atttgtcttt
      601 gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg gcacgccact
      661 ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg tatcaattgc
      721 gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt ctttcagacg
      781 gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat gctattttcg
      841 ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa acgcagagga
      901 tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag cgttaactcg
      961 aggcttctta ata
//
LOCUS       DAJTYF010000311          967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTYF010000311 DAJTYF010000000
VERSION     DAJTYF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.318"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(133..810)
                     /locus_tag="OO590_004319"
     CDS             complement(133..810)
                     /locus_tag="OO590_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639407.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
BASE COUNT          254 a          223 c          211 g          279 t
ORIGIN      
        1 cgcctctgta gtattaacgt atttgatgtt gtttggcagt tttacttgtt tcgccgtagc
       61 gaagacagag aacagaatag ttacagcaac gagtaaagtt ttgataaaag ttttcatgac
      121 agactcctta aattagttaa ccgcagctac gtagtttttc accagatacg cgaattcatg
      181 cacgccaatt tcgccgaacc aaaccaggta ggccgggtca tctaaaccaa agtagcggta
      241 gccaacgact tcatgggttt ttgggttata gagtgttacg ataaagtccc agccgcaggt
      301 attggctttg caaccgtcgc cctggagcca gactgtaccg ttaatggtta ctggcgttac
      361 gtttccgcca gggccatcca tatacccgcc tttaccgaac atgccgctca gtccgaccgg
      421 ttttaatgct ttgtcaagcg cagctttgta ggcagcgtca ccttcgtaca actcgtacgt
      481 tgttggacac gctccaggtt gttcagcccg ggcgtttggg tcgcagctga gcgcttgttt
      541 cacgctcgct gcttccacgt taaagccagt aaacagaacc gccagaaata acatcagggt
      601 atgtaaacgt ttcattgtaa atattcttcc gttgtaaatg gcgctggaaa atttctcatc
      661 agtgaaacct tccggaaatt tccccccctt gtatgatgta tatcgccagt attttatttc
      721 atcccggata taatttttat atttcccggc acgtgcaata acgctgacca gagatataag
      781 gctcattaat aactttacag gtaattgcat aattaaattt ccagtgagtg aattgacagg
      841 tgaggggtaa aactttctct caggtaatat atatctgaaa atattttcaa taacaccagc
      901 taggaaaaac accaccacag gcggtggcgt ttctttggag cgtctttatt taatccgggc
      961 catcgcg
//
LOCUS       DAJTYF010000312          967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTYF010000312 DAJTYF010000000
VERSION     DAJTYF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.319"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            77..>967
                     /locus_tag="OO590_004320"
     CDS             77..>967
                     /locus_tag="OO590_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR6639408.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGGIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKAQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTMYNDVLFPG"
BASE COUNT          257 a          229 c          241 g          240 t
ORIGIN      
        1 gtgcgctgtt gtccatgtga tattgtgtac cagatgtgtg ccaaactatt atttctgagc
       61 gtaccaaata ttatttatgg ctataagcga cacaaaactg cgtactattt atggtaaacc
      121 atattcgggc ccacaagaag tggctgatgc cgatggcctc agcgtacgaa tttcaccgaa
      181 ggggggcatc cagttccagt accgctatcg ctggcatggc aagcctaatc gacttgggct
      241 tggtcgatac ccatccctgt ctttgaagga tgccagacag atcactgctg acttgcgaaa
      301 gctctatttc tcaggaacgg atccacgcac ctattttgaa gagaaggtgg agaactccat
      361 gacggtcgcc cagtgtctcg actactggtt cgacaactac gtctctacaa ctctcagaga
      421 aaaggcccag gcactttatc gatcaacggt tatgaagcgc atgcatgacg ccttccctaa
      481 tcgtccggca tcttctatca cggttaagca atgggttgac ctgcttaccg aagaagaaaa
      541 agataatcca cgccgagcaa ggcaggtgct aagtcaacta agatcagcaa ttagttggtg
      601 catgcggcgt cagttgatag atagttgcgc aattatgagc atccagccaa gggacttcgg
      661 ctcccgcgct gaggtagggg atcgggtact gtcgtatcac gaactggcta agatttggct
      721 tgctattgaa agaagccgtg cgtctacgtc aaataagctc cttcatcaga tgcttatgct
      781 gtggggggcg aggctctcag agcttaggct ggcaacaaag acagaatttg acctgctgga
      841 caacgtatgg accgtaccga aagagcatag caagatgggt aatgttatcc gccgtccaat
      901 cttcgaacaa attaagcctt ttctcgaaaa ggccatgaca atgtacaatg atgttctttt
      961 ccctgga
//
LOCUS       DAJTYF010000313          965 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTYF010000313 DAJTYF010000000
VERSION     DAJTYF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.320"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>965
                     /locus_tag="OO590_004321"
     CDS             <1..>965
                     /locus_tag="OO590_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6639409.1"
                     /translation="YLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSLRTKDRR
                     TAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPESYQLYVD
                     QYDDIKSNLREAVATEHLTVDQHRYINDVIGVLKACQDRLKGDSSGLLSYLEPETGSL
                     RPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTLIEIFDY
                     LDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDVVTINNK
                     YLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPE"
BASE COUNT          249 a          260 c          228 g          228 t
ORIGIN      
        1 tacctttatc agcgtaatgg tgtcttttac atccgtctta ggatgaagac cactggtcgc
       61 cttaccgctt cgcttccttc tcacaaccgt tataaactag cgtcagtatc tttacggacc
      121 aaggatagac gtaccgctat ggctcactcc cggcacatca agtcagcact taaagcaatc
      181 catgcagaca accctaacgc ctcttatgag gagctacggg agcacctgaa gaccattgtc
      241 gagtgggaac tcagtgtcag ccgcgatgac ctgaacgacc ctgagtccta tcagctttac
      301 gttgaccagt acgacgatat taagtccaac cttcgggaag ctgtagcgac tgagcacctg
      361 actgtagacc agcatcgcta tatcaatgac gttatcggtg tgcttaaagc ctgtcaggat
      421 agactcaaag gtgatagctc tggcctattg tcttaccttg agcctgagac tggcagtcta
      481 agaccgtccg tctccctatc tgtattggcg gaacctgaag tccctgaacc taaagcactg
      541 accctcgcgt ctctcattga gcagtacgag caggagaacg cccagaactg gaagccagcg
      601 accctaagcg aaaaccgagc gtcacactct acgctaatcg agatattcga ctatctggat
      661 attcaggatg tcggcaaggc gacccgtgca gatatgctca gagttcgtga agtcctccag
      721 cagctaccga agaaccgcaa gcaacgcttt aagtctatgc cgctgtctga cttgctgaac
      781 cgggagtcta agactgactg tctggacgtc gttaccatta acaataaata tcttatcaag
      841 atggctgctg tgtttaagtg ggcggtacgc aacgacttga tagctaagaa cctgaccgaa
      901 ggtcttgagc tgaaagtccc tcagcgtaaa gcctccgacg ctcgtgatgc gttcagtcct
      961 gagca
//
LOCUS       DAJTYF010000314          901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTYF010000314 DAJTYF010000000
VERSION     DAJTYF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.321"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..97)
                     /locus_tag="OO590_004322"
     CDS             complement(<1..97)
                     /locus_tag="OO590_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6639410.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQ"
     gene            complement(84..455)
                     /locus_tag="OO590_004323"
     CDS             complement(84..455)
                     /locus_tag="OO590_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6639411.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMISRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(467..868)
                     /locus_tag="OO590_004324"
     CDS             complement(467..868)
                     /locus_tag="OO590_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6639412.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
BASE COUNT          214 a          277 c          195 g          215 t
ORIGIN      
        1 tctgtgccac acggtttatc gcccatgcgc tggcctgtgg caccatacgg gtatcaaggc
       61 tgttcagatt gcggatggca ttctcaagcc ccttcataat atcgtcctgc ccttacgttt
      121 cccggcagcc cctcacgccg caaccggata taactacatc cgccatcatc aggggtgata
      181 cgatccacac agaaagactc acgactaatc atcagcgaat cattgcgaca cagccgtaaa
      241 atatctgctg ttttcacaaa cagggatggt aaagagtctt caatacgcac accgccggcc
      301 accagagaaa tattttcagg atcatcaaaa accccggaaa tcatgatccc ttttagccgg
      361 cctgacgtaa tgaccgctct aatccccata ttgcgaataa tggccttgtc tgcgagatca
      421 agtgcaacat caaacagatt atcgaaatca gccacgctgc ctcccgttat tgcattctgg
      481 ccaggccacg ttctgtcatt ccggctgcca caccggcaga gacacggaac gccattcccg
      541 gcggcacaaa tgccagaggt tcatcccgcg tggcatgaag tgcatcggta tgcagcgtca
      601 ccagtgccac gactgtaacc agatcatcca tattctgaat cacggtctcc ggctgcgctg
      661 atatcacctc attttcatgt ccggtcagcg cattttccgg gtgggcagac ttatcccgga
      721 tgtcagtgtc atccatattg gcaagttcct cctccagctc agccacacgc tgcgcaatat
      781 ctgctgcgct tcccgagata tccggctcac gtcccagcga tgctgccagt tctctcagac
      841 gtttcaggtt atcgtctttc tttgccatat ctgttcctgt ccgaataaaa aacgggagcc
      901 c
//
LOCUS       DAJTYF010000315          863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTYF010000315 DAJTYF010000000
VERSION     DAJTYF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.322"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..>863)
                     /locus_tag="OO590_004325"
     CDS             complement(<1..>863)
                     /locus_tag="OO590_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6639413.1"
                     /translation="VIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPY
                     ASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGS
                     AALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYR
                     YSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWH
                     TDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLS
                     GWWGNSYATYSV"
BASE COUNT          225 a          222 c          196 g          220 t
ORIGIN      
        1 acagaatagg tggcgtaact attaccccac cagcccgata aaggaacaga taaagagagc
       61 atgtagatct gctcactggt gttatcactg gcatggttgt tccccgtccc ctgtctggca
      121 gtattggaat aactccagga gacgttccac gagagacgct tccagctact gctgtagcca
      181 aattgcatta atcgttcgac atcatcggta tgccagtatt gttgccggct taatgtcagg
      241 tataaattgc cataatctct ccccaacgac tgcgttagcg ttccctccac gcgactacgt
      301 cggttacctg tttcccaaaa gtcttcaggg ctgttgcgcc gcgatgccca ctcattgagg
      361 gtatagtagc cctcggtgga gtaacgatag ccaaccaggc gcaagctagt gtctgtgtcg
      421 tcaaatagtt ttgaatagag aaagcgatat gactgacccg tttctgtatc atcatggcta
      481 aaattcgcac gagcatgtgt cacatcgaaa gaaattgcgc ccaaagcgcc aagatcttta
      541 ccgacaccca gagctgcaga accataattt tcagcagcct gcatacctcc atataaagtg
      601 atcccgtggg aaaaaccatg aagtatttgc gcctgaagga cgtccggtgt aaatccgtct
      661 tcatcgcgca attcacccac gctgacatca acatctgtca gaccttcacg tttaagaatc
      721 gccaatgaag cataaggttg ggtgaatcgt tgttcgctac cgtcttcttc tttgatggtt
      781 acgtccaaat cgccacctgt acttgcggtg ttgagatcgg tgatctcaaa agcaccttgt
      841 ggaacggctg actgataaat cac
//
LOCUS       DAJTYF010000316          856 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTYF010000316 DAJTYF010000000
VERSION     DAJTYF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.323"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            89..778
                     /locus_tag="OO590_004326"
     CDS             89..778
                     /locus_tag="OO590_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6639414.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTAHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
BASE COUNT          249 a          166 c          235 g          206 t
ORIGIN      
        1 acgagcagtt tgatgaaacc aatattgtgc gcggactgcc tgttcctggt ggtcggttgg
       61 ggataaaaat cacagagctg gagtgtgcat gaataaccag tatttacagt ttgttcgtga
      121 gcagctcatt atcgccaccg ctgatttgag tggggcgaca aaaggtcagc ttgaagcctg
      181 gcaggagaat gccatgttcg atacagggcg ttacaggcgt aaaaaaatcc ggtaccgcga
      241 tgaggtgacc ggaaaaatga taacgcggga taatccacca atcccgggaa aacaatcact
      301 ggcgaagggg gcgtcaattc ctctggtcag tcaggttgcg ttttcgacat catcgtggcg
      361 gcgggctgtt ctgtctcttg aagagcacca taaagcctgg ttgttgtggt gttacagcgg
      421 aagtatttgc tgggagtatc agatcaccat aacccactgg gcgtgggaag agtttaaggc
      481 tcattctggc actaggaaaa ttgcagagaa aacacaggaa cgcctgaaaa aattaatctg
      541 gctggcggcg caggcggtaa aagcagaact ttttggtggg gaaggttatg aatatcagga
      601 gctggcatta ctggcgggag taacaaccaa aaactggtcc aaaacattta ctgctcactg
      661 ggttgtaatg aaacacattt ttcaccgact ggatagtgag gctttattgt ttgtgatgag
      721 aacacgttca gaacaaaaag cggcattttc aaagcaaagt attgcaaaag tagattaaaa
      781 ggcatatatt tcatgcaaat ctgatacttt gccgattttg tacgcgatgg taaagtaagc
      841 aaaacccgcc gccgag
//
LOCUS       DAJTYF010000317          840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTYF010000317 DAJTYF010000000
VERSION     DAJTYF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.324"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..438)
                     /gene="uidR"
                     /locus_tag="OO590_004327"
     CDS             complement(<1..438)
                     /gene="uidR"
                     /locus_tag="OO590_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6639415.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEI"
     gene            complement(667..>840)
                     /locus_tag="OO590_004328"
     CDS             complement(667..>840)
                     /locus_tag="OO590_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6639416.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT          202 a          206 c          199 g          233 t
ORIGIN      
        1 tatctcgcct ttttgctggg catcacgcat ccgctgggca acaaattcag tgatcgtcat
       61 atgcttattt ttaagcatgg cggcgacctg tgggttacgc atcccttccg ccataatttc
      121 gaccaccagc gcccgttgcc caaaggcttc atgggtgaga gagacaatag actcgaccat
      181 atagtcaaca aaatgaatcc cttcaatcgg ttcccggaaa cgagccagcg ccctctcctg
      241 gtcctgtaag ataatcgcct gaatcaaggc ttctttggag atgaaatggt gatagagcgt
      301 cccaggacta atggcgcaag atttacagat ggctttcatc gaggcactgt gaaatccatt
      361 ttctgaaaaa atctctctgg cagcattgag gatccgggtc cgtgtcggtt gtgcttcagt
      421 ctgcatgtta tccatcatta aaccattata gttagcgtga ataactagtt taccgcgttg
      481 tcaggtgagc gcctatcatt gctgttgaat agtgcgaagg tacactctat tcgtatactt
      541 ttcacccagc aattggttaa ccaattgccg tgatttggct gccagttgtt tagcagagac
      601 gttgatgaat tgcacacgct atcccggtaa acaacacata cgaccgttta ccggttcgtt
      661 agtgtattaa ttgagctcct gtaccccacc accggagacg gtgagaattt gtccgcttac
      721 ccagctggct gcaggcgagc aaaggaacag cgccgcgtta gcaatatctt gcggttggcc
      781 cagacgtctg atcggcgtgt gctgtaacat tttttgttca atttctgggt aatgactcca
//
LOCUS       DAJTYF010000318          830 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTYF010000318 DAJTYF010000000
VERSION     DAJTYF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.325"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..326
                     /locus_tag="OO590_004329"
     CDS             <1..326
                     /locus_tag="OO590_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6639417.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="OO590_004330"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="OO590_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR6639418.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRLVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          255 a          181 c          153 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacggccaga
      481 atatcgacca agcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag aaaaatgtaa
      781 gaagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga
//
LOCUS       DAJTYF010000319          803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTYF010000319 DAJTYF010000000
VERSION     DAJTYF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.326"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..244)
                     /locus_tag="OO590_004331"
     CDS             complement(<1..244)
                     /locus_tag="OO590_004331"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6639419.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQSATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
     gene            complement(241..663)
                     /locus_tag="OO590_004332"
     CDS             complement(241..663)
                     /locus_tag="OO590_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6639420.1"
                     /translation="MWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTP
                     PEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVR
                     ESNGEEQHFSVPFQTPAIALHEGYFKYSVMGGNIKKKV"
BASE COUNT          222 a          219 c          151 g          211 t
ORIGIN      
        1 attgagtacc aaaatcacta atggatttac tataacgaat acgatattta ttcccataat
       61 ataaattcgc aatcgaacgc gtaatatctg tcgataaact ccctaacagc cccagagaaa
      121 tgccaatacc cgtagatatt gcattaaaat gagtggcgct ttgaatccct ccatacaccg
      181 tgacgccata aggaagcccg tacatcgccg tgatttcgcc cacggagaca ggggagcttt
      241 ttataccttc ttctttatat tcccccccat cacactatat ttgaaatatc cttcatgcag
      301 agcgatggcc ggcgtttgaa atggcacgga aaaatgctgc tcctcgccat tactctctct
      361 cactgtaaca tcaagctccc cgccaacggc cagcgttggt aaattatcca gcgcaaaagg
      421 ccctggcggc acagaacggg taaaaataat ttgcccattt tgtttaataa taacctgcgc
      481 ctcactttgt gctattccat gcaccacagg tgtgtagtag ctgtcctccg gcggagtcat
      541 cgcatcgtcg gtcgctaaca tcccgccgcg aaaaggtatg ctgtcaaaca cctcagacga
      601 cgtataagat tctccaagcg ttaatcggct tttaatcgca tttatgcctc gctcaaacca
      661 catgtaaccc ctttgccatc tttctgactg gccaagttcc ttgctccatg tcactgaact
      721 acgaaacctc caggcaccaa gattaaaccc tggagtgaac ctgccataac gagaattacg
      781 cgtggtagat tgattatcat ttt
//
LOCUS       DAJTYF010000320          794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTYF010000320 DAJTYF010000000
VERSION     DAJTYF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.327"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            43..>794
                     /gene="ipaH3"
                     /locus_tag="OO590_004333"
     CDS             43..>794
                     /gene="ipaH3"
                     /locus_tag="OO590_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR6639421.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          236 a          204 c          130 g          224 t
ORIGIN      
        1 tatttctttt aacaaagcca tttgtccatc ggctttaact gaatgtccat catgttaccg
       61 ataaataata acttttcatt gtcccaaaat tctttttata acactatttc cggtacatat
      121 gctgattact tttcagcatg ggataaatgg gaaaaacaag cgctccccgg tgaaaatcgg
      181 aatgaagcgg tctccctact taaagaatgt ctcatcaatc agttcagtga gcttcaactg
      241 aatcgtttaa atctgtcctc gctacctgac aacttaccac ctcaaatcac tgttctggaa
      301 attactcaga atgccctaat atcattacca gaattgccag catcgctgga ataccttgac
      361 gcctgtgaca atcacctgtc aacacttcct gaattacccg catctctgaa acatcttgat
      421 gtagataaca accaactaac catgcttcct gaattgcctg cattgctgga atatattaat
      481 gcagataaca atcagctaac catgcttcct gaattaccta catcgctgga agtgctctca
      541 gtaagaaata accagctgac atttctccct gagttacctg aatcactgga agcgctcgat
      601 gtaagtacta atcttctgga aagcctacca gccgtacctg taagaaatca tcactcagag
      661 gaaaccgaga tatttttccg gtgccgcgag aatcgcatca cacacattcc ggaaaatata
      721 cttagccttg atccgacctg cactatcatc ctcgaagaca atcctctgtc ctcacggatc
      781 agggagtctc tgtc
//
LOCUS       DAJTYF010000321          780 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTYF010000321 DAJTYF010000000
VERSION     DAJTYF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.328"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..347
                     /locus_tag="OO590_004334"
     CDS             <1..347
                     /locus_tag="OO590_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6639422.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
     gene            702..>780
                     /locus_tag="OO590_004335"
     CDS             702..>780
                     /locus_tag="OO590_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein DinI"
                     /protein_id="HCR6639423.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEE"
BASE COUNT          228 a          182 c          174 g          196 t
ORIGIN      
        1 cccccgctaa agtgttgccc gaaaactggt ggcaacatcc agctgcatta ggtgcaactg
       61 acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg gatctggagt
      121 tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg tgggatctcg accaatatcg
      181 gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg attgccgaag
      241 acgcctgtag tgccgccagc gccgagcaac acaataacag cattaatcat atctacccgc
      301 gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt acatcggtct
      361 accgcaatgg tcgcatccta aatgggtgcg gttggggatc accagccttg aagagtatgc
      421 ccgccacttt aactgcgtga tacgggattt ttaaaaataa ctaaagaacg cccaagagca
      481 tgtgttttct ttagaataat caaagcgttg tattgccatg tcatatgaaa atcggcaaag
      541 ttcatatgta acaaagtgtc ccaatcatgc cccaaaatgc cccacaacaa atatttttgc
      601 cccatgcatg ccccaaaaaa tcactttccc tcactcttga cactgtttat ccatacagtt
      661 aaaaataata ctgtataaaa acacagtgta gagggatttt tatgcgtatt gaaatctgca
      721 tagccaaaga gaaaatgact aaaatgccaa ccggtgctgt ggatgcgtta aaggaagaat
//
LOCUS       DAJTYF010000322          759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTYF010000322 DAJTYF010000000
VERSION     DAJTYF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.329"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            58..555
                     /locus_tag="OO590_004336"
                     /pseudo
     CDS             58..555
                     /locus_tag="OO590_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            596..759
                     /locus_tag="OO590_004337"
                     /pseudo
     CDS             596..759
                     /locus_tag="OO590_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          191 a          192 c          222 g          154 t
ORIGIN      
        1 cgccagcccc gtcccgcccg atagagcgcg gcatagcggg ccccggatta cttgcgtgcg
       61 acggcagaag aagccctgaa gcgaatcagt gaactgtacg ccatcgagga tgaaatacgg
      121 ggattaccag agtcagagtg tcttgcagtc aggcagcagc gaagcaaagc gttactgacg
      181 tcgctgcatg aatggatggt ggagaagaat ggcacgctgt cgaaaaaatc cagactgggc
      241 gaagcattca gctatgtact gaatcagtgg gacgccctct gttattacag tgatgacggt
      301 ctggcggagg cggacaataa cacagcggaa agagcgcttc gtgcagtctg tctcggaaag
      361 aaaaattacg tgttcttcgg tagcgatcac ggcggcgagc gtggtgcact gctgtacggg
      421 ctgatcggca cctgccgtct gaacggtatc gatccggaag cgtatctgcg ccatattctg
      481 agcgtactgc cggaatggcc ttccaaccga gttgacgacc tcctgccatg gaaggtggtg
      541 ctaccatcag gataatcgtc aatacggtgt tcggtgaacg tttacttgga gcatcatgct
      601 tcatgaagaa aaactggcac gtcatcaacg taaacaggcg atgtataccc gaatggcagc
      661 cttcccggcg gtgaaaatgt tcgaagagta tgacttcacg ttcgccaccg gagcaccgca
      721 gaagcagctc cagtcgttac gctcactcag cttcataga
//
LOCUS       DAJTYF010000323          754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTYF010000323 DAJTYF010000000
VERSION     DAJTYF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.330"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>754
                     /locus_tag="OO590_004338"
     CDS             <1..>754
                     /locus_tag="OO590_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6639424.1"
                     /translation="RILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFELD
                     LEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGADSWAGTD
                     LFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDG
                     FSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDV
                     VNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDV"
BASE COUNT          209 a          155 c          181 g          209 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcgctgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgt
//
LOCUS       DAJTYF010000324          748 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTYF010000324 DAJTYF010000000
VERSION     DAJTYF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.331"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(570..>748)
                     /locus_tag="OO590_004339"
     CDS             complement(570..>748)
                     /locus_tag="OO590_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6639425.1"
                     /translation="YKDSDRLNYIEIGTWYYFNKNMNIYTAYQINLLDKSDYVLAHGL
                     NTDDQLAVGIVYQF"
BASE COUNT          204 a          119 c          194 g          231 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gattctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagcggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaac
//
LOCUS       DAJTYF010000325          745 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTYF010000325 DAJTYF010000000
VERSION     DAJTYF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.332"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..734
                     /locus_tag="OO590_004340"
     CDS             <1..734
                     /locus_tag="OO590_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR6639426.1"
                     /translation="YVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADA
                     YAGYNVLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGS
                     PAEERLAVRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDG
                     WVEIDNNIGENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLRE
                     VIEKLNDWPSNQVHELLPWNFSSVK"
BASE COUNT          202 a          172 c          226 g          145 t
ORIGIN      
        1 tatacgtcag ggatgatcgt aatgcgggtt catccctgcc ggcagccgtc tggttcgcgt
       61 attcggcaga tcgcaaagga gaacatccgc agctccacct ggcaaagtat cagggcgtac
      121 tgcaggctga tgcctatgca ggttataacg tactgtacga aacgggccgg gtgaaggaag
      181 ccgggtgcct ggcccacgcc cgccgaaaaa tccatgacga ggatgtgcgc cgtccgacag
      241 aaatgactca ggaagcgctc agacggatag cagagttata cgacatagaa gcggagatac
      301 gtggcagtcc ggcagaggaa cggcttgcag tcagaaaagc cagaagcgtc cagttgatgc
      361 agtcgttgta cgactggata cagttgcaga ggaaaacgct gtcgaaacat gcggagatgg
      421 cgaaggcgtt cgactatatc ctgaatcact ggaatgcgct gaacgagttc tgtcgtgacg
      481 gctgggtgga aatagacaac aacatcggtg aaaacgcgtt acgatcggtg gcggttggaa
      541 gaaaaaatta tctctttttc ggctcagaca agggaggaga aagtgcggcg atcatctaca
      601 gtctgctggt cacctgcaaa cagaacgaag tggagccgga ggactggttg cgcgaagtga
      661 tcgagaagct caatgactgg ccgtcgaacc aagtgcatga actgctgccc tggaacttct
      721 cgtctgtaaa ataatcctta cgcta
//
LOCUS       DAJTYF010000326          741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTYF010000326 DAJTYF010000000
VERSION     DAJTYF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.333"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..562)
                     /locus_tag="OO590_004341"
     CDS             complement(<1..562)
                     /locus_tag="OO590_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6639427.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVA"
     gene            570..>741
                     /locus_tag="OO590_004342"
     CDS             570..>741
                     /locus_tag="OO590_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6639428.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSNVCAYFE"
BASE COUNT          181 a          186 c          172 g          202 t
ORIGIN      
        1 tagccacgcc agcatccagc ccgaccattg atgctgaagg gtgagccgga gtggatactt
       61 cactttctgt ctgaatactg atgtaccacg taccgcagga ctggctgaca gtgacatttt
      121 tcacaacacc cgtgacctga cggctattcc ggtagcgcat ccagcccagt ttcggcagaa
      181 aaatacggct gttttcctga tcgagcttaa cgccctgcgg gtagcggaat gcatcattct
      241 gtccccgctt tttgaatcgg ggaaaagcag cccgcttccg gaagaagttt ttgtaagccc
      301 gctcaaggtc tttcagtgac tgttgcaatg gctgtgaggg agaatcttta agccattgcg
      361 tttcagtggc atttttccac tcaaccagcc aggaagccat tttcccgtaa gggatgtatt
      421 tattaccggc ctcatgattc tcattctgac gtgccagagc acgattgaaa acgaaacgac
      481 atgcgcctgc gaagcgcctc atttcacact cctgttgacc acctggtctt aactggaatt
      541 taaatgcctg taatcgtttc acaggtcaaa tgatactttg gtctgtgaaa aaagaaaccg
      601 atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc tttgtcacca
      661 gataccgacg ccagattttt gaccatgacg cgacagaaaa actacgcact tacttttcaa
      721 atgtatgtgc ttattttgaa g
//
LOCUS       DAJTYF010000327          738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTYF010000327 DAJTYF010000000
VERSION     DAJTYF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.334"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..452
                     /locus_tag="OO590_004343"
     CDS             <1..452
                     /locus_tag="OO590_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6639429.1"
                     /translation="NRKKELTEPQIREGSWRKERRTSYSREFKVRLAKQALQPGAVVA
                     RIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNK
                     HDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            449..>738
                     /gene="tnpB"
                     /locus_tag="OO590_004344"
     CDS             449..>738
                     /gene="tnpB"
                     /locus_tag="OO590_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6639430.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLL"
BASE COUNT          190 a          189 c          210 g          149 t
ORIGIN      
        1 ccaacagaaa gaaggaatta acagagcctc aaatcagaga aggctcatgg cgaaaagaac
       61 ggcgaaccag ctatagccgt gaatttaagg tccgtctggc taagcaggcg ttacagcctg
      121 gggctgttgt tgcccggatc gccagagaac acgatatcaa taataacctg ctgtttaaat
      181 ggaaaagcca gtacgaggac ggcttactga gcgatgatga catacaggaa tgcatgcctg
      241 tcccggtggc actgactgat acgccggagc cgaccagacc agttacaaat cccttctggc
      301 gtaacaagca tgatgagcgc cctgaggggg ctcccggaaa cgtcccacgg tgcgagctgc
      361 atcttaaatc aggtgtggta aaactgtttg accctctcac tccggaactg ttacgggcgc
      421 taatccgcga aatgaaaggg ggtatccgat gataacgctg ccgaccggta ccaaaatctg
      481 gatcatcgct ggcatcacag atatgcgttg tggcttcaat ggcctggctt cgaaggtgca
      541 gaacacgctg aaagatgacc cgttctccgg gcatatcttc gtcttccggg gccgcagtgg
      601 caaaatggtg aaaatactgt gggccgatcg tgacgggtta tgcctgttcg ccaaacgcct
      661 ggaacggggc cgcttcgtct ggccggtgac ccgggaaggg aaagtgcacc tgacgccagc
      721 tcagttatcc atgctact
//
LOCUS       DAJTYF010000328          723 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTYF010000328 DAJTYF010000000
VERSION     DAJTYF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.335"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>723
                     /locus_tag="OO590_004345"
     CDS             <1..>723
                     /locus_tag="OO590_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR6639431.1"
                     /translation="PLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKT
                     AQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQ
                     MELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEH
                     RHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKV
                     CHLLTSMTDAMRFPGGEMADLYS"
BASE COUNT          181 a          209 c          206 g          127 t
ORIGIN      
        1 cctcttcacc agattgtgaa tcgcctggac atcatgctgc cgggcaatcg ccccttcgtt
       61 gcccccagtg ccgttattca ggcccgccag cgcctgggaa gtgaggctgt ccgccgcgtg
      121 ttcacgaaaa cagcgcagct ctggcataac gccacgccgc atccgcactg gtgcggcctg
      181 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
      241 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      301 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      361 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      421 atggataaag gttattactc actgggactg ttaaatggct ggagcctggc gggagaacac
      481 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      541 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      601 ctgggaaatg aagtgacagc ccgcctgctg accgtgacgc gcaaaggaaa agtctgccat
      661 ctgctgacgt cgatgacgga cgccatgcgc ttccccggag gagaaatggc ggatctgtac
      721 agt
//
LOCUS       DAJTYF010000329          713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTYF010000329 DAJTYF010000000
VERSION     DAJTYF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.336"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            157..>713
                     /locus_tag="OO590_004346"
     CDS             157..>713
                     /locus_tag="OO590_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6639432.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLMVSGNRLDS"
BASE COUNT          251 a          131 c          129 g          202 t
ORIGIN      
        1 gggtctggct ggctaacacc ccattataag gggtacgaat aaagtaacgg gggaagcaat
       61 acctggagaa agagtacaaa gcaaactgta ttaaacttaa taagttatat tgtatttcac
      121 tagagttctt atcgttgata agaaattctg gttataatga ttaaatcaac aaatatacag
      181 gtaatcggtt ctggtattat gcatcaaata aacaacatac actcgttaac tctattttct
      241 ttgcctgtga gtttgagtcc atcttgtaat gaatattatt taaaagtctg gagtgaatgg
      301 gaaaagaacg gtaccccagg cgagcaacgc aatatcgcct tcaataggct aaaaatatgt
      361 ttgcaaaatc aaggggcgga attaaatcta tctgagttag atttaaaaac attacctgat
      421 ttaccgcctc agataacaac actggaaata agaaaaaacc tattaacata tctccctgat
      481 ttcccaccaa tgcttaaggt aatacatgct caatttaatc aactggaaag cttacctgcc
      541 ttgcccgaga cgttagaaga gcttaatgtg ggtgataaca agataaaaga attaccatat
      601 cttcctgaaa ctctaactca tttacggatc cataataacc gattgcatat tctgccacta
      661 ttgccaccgg agctaaaatt actgatggtt tctggaaaca gattagacag cat
//
LOCUS       DAJTYF010000330          702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTYF010000330 DAJTYF010000000
VERSION     DAJTYF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.337"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            36..>702
                     /gene="istA"
                     /locus_tag="OO590_004347"
     CDS             36..>702
                     /gene="istA"
                     /locus_tag="OO590_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR6639433.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHHNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLC"
BASE COUNT          161 a          180 c          185 g          176 t
ORIGIN      
        1 agggggtaat ctgctctcct gattcaggag agcttatggt cacttttgag acagttatgg
       61 aaattaaaat cctgcacaag cagggaatga gtagccgggc gattgccaga gaactggggc
      121 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
      181 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      241 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      301 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      361 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      421 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      481 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      541 ataatgcgtt ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa
      601 ctgtggttct gcaacgtgac gcatatcaga ccggtcagca ccggttccat ccttcgctgt
      661 ggcagttcgg caaggagatg ggcttctctc cccgactgtg tc
//
LOCUS       DAJTYF010000331          692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTYF010000331 DAJTYF010000000
VERSION     DAJTYF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.338"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..489)
                     /gene="yidZ"
                     /locus_tag="OO590_004348"
     CDS             complement(<1..489)
                     /gene="yidZ"
                     /locus_tag="OO590_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6639434.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELL"
     gene            complement(464..>692)
                     /locus_tag="OO590_004349"
     CDS             complement(464..>692)
                     /locus_tag="OO590_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Confers resistance to chloramphenicol; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug transporter subunit MdtL"
                     /protein_id="HCR6639435.1"
                     /translation="LGPFSLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGI
                     LIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
BASE COUNT          168 a          202 c          176 g          146 t
ORIGIN      
        1 taacagctcc cgcgagcgag gatggctttc gcgaccggaa aaaccaatat ccacttcacc
       61 acgagtaatg gcatctaagg aatcgtaatc ccagttacgt agtttgatgg tcgcctgcgg
      121 gtaacgttgg tagatccgtt tcgacagcgc attaagcatg atcatcatca gcggcgattc
      181 cgccgccaac tcaaacttca ggccgcgcgg tgtctggtgg tgcggtttat ccagcagcag
      241 attgctcatt tgcatccatt ccgccagatt ttgctccatg ctgaccatta gctgtgtggg
      301 cgacagcccc agcggtgagt tcacaaagag cgggtcgtca aaccacgctc ttaactttgc
      361 cagcgactta ctcaccgctg aaggggtcac gttcatccgc ttcgccgctt tggtcacgct
      421 gcgctcctgc atcagcagtt gcagacagag cagcagattg agatcaagcg tggtgatgga
      481 tttcttcatg agcggcaacg gggcgtccag gcgcgacgaa cataatcagc aacaggctca
      541 ctatgctaca ggcaatcaga atcccgatca gcatattcca tgcgccgata ccaaccaccg
      601 cggccagcca aatccacagt gacgaaccgc aaacctgcgc aatacctaag gtcgagctgg
      661 ctacgcccgc gcgtaatgaa aatggtccta ac
//
LOCUS       DAJTYF010000332          664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTYF010000332 DAJTYF010000000
VERSION     DAJTYF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.339"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>664
                     /locus_tag="OO590_004350"
     CDS             <1..>664
                     /locus_tag="OO590_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639436.1"
                     /translation="WRRFTDRFVNSQAHIIMCGRAGYEYDFFEGDDGKRQLAKTGIKM
                     KAETETGYEPSILIQMEKQMNIETGQVWRTARILKDRSTRIDGQVFSNPTFKNFLPHI
                     ESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRAKEIALDEIIELLNKHHGGTSND
                     AKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGVEYAFPDPQTQNETQQ
                     AGY"
BASE COUNT          222 a          152 c          154 g          136 t
ORIGIN      
        1 gtggcgacgc tttaccgatc gatttgttaa cagtcaggcg cacattatta tgtgcgggcg
       61 tgcaggatat gagtatgact ttttcgaagg tgatgatggc aaacgccagt tggcaaaaac
      121 cggtataaaa atgaaagcag aaacagaaac cggttatgaa ccctccatcc tgatccagat
      181 ggagaagcaa atgaacatag aaaccggtca ggtgtggcgc accgccagaa tactaaaaga
      241 tcgctccacc cgtattgatg gacaggtatt ttcaaaccca acatttaaaa actttcttcc
      301 tcatatcgag tctcttaacc tgggaggtga acaccccggc atcgatacct cacgggataa
      361 cagtgaatta tttgccaacg acggtacacc gacatggcta aaagaaaagc gtgcaaaaga
      421 aatcgcactt gatgaaatta tcgagttgct caacaagcac catgggggca ccagtaacga
      481 cgccaaacgc gcaaaagctg atttgctgga aaaaacattc acctcccgct catgggaaag
      541 aatcaaaggt atggactggc cgacaatcaa agctggtcgt aatgcactat ggattgaact
      601 ggaaggcgtt gaatatgcat tcccggatcc acaaacacaa aacgaaacgc aacaagcagg
      661 atac
//
LOCUS       DAJTYF010000333          661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTYF010000333 DAJTYF010000000
VERSION     DAJTYF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.340"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(105..235)
                     /locus_tag="OO590_004351"
                     /pseudo
     CDS             complement(105..235)
                     /locus_tag="OO590_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(233..328)
                     /locus_tag="OO590_004352"
                     /pseudo
     CDS             complement(233..328)
                     /locus_tag="OO590_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          189 a          123 c          159 g          190 t
ORIGIN      
        1 cttccttatg ttatattttg gtaaaaaatt tattttttta cattatagtc ggctaaatga
       61 agtttactgt aaaaaaacga tactttttcg ggtggcgctt ccaatcagta gaatatgtgt
      121 ttttcaatga aggagccgat gacttttcgt ggatttccac ggaacgcgag aagcagattg
      181 ttttacgtgc cagccgcttg atgcgggtgc ggagggtcag gttgttacgt tcaagatata
      241 gcatgtgctg gatgaagatc ccttttctgg tcacctgttt atctttcgtg gccgacgggg
      301 tgacactgtt aaaatgtaat gaacgcatcc cacacaatta aacacggagt agtatgatgg
      361 ttaaaagggt gaaactggca ggtgtggagc ctcgcagctt tccggcatca tactgcccaa
      421 tacgggatgc ataaagcgtc ctcagactgg agactccaca aatgaaatat acaccggttg
      481 gcgttgatat ggcaaaacat gtcattcaga ttcacttcat caatgagcac atgtcagcgc
      541 cagtgatata agacggtaat ggggatgcgg acgatttcct ggactctcag aagatgaaac
      601 atgtttactg aagaacaaaa aattcgcgct attgagcttt atttcaaata tggtaagaag
      661 c
//
LOCUS       DAJTYF010000334          657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTYF010000334 DAJTYF010000000
VERSION     DAJTYF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.341"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(341..>657)
                     /locus_tag="OO590_004353"
     CDS             complement(341..>657)
                     /locus_tag="OO590_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6639437.1"
                     /translation="KKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKD
                     GANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRF
                     EI"
BASE COUNT          187 a          137 c          112 g          221 t
ORIGIN      
        1 cattattggc tccttctgat gccattctat ttcaggaagg agtgtccgtt aaactcaggc
       61 tacctcactc ccgtaaagac aggagtcaag ttcccgctcc gacataactg cgtgcaatgg
      121 attttgaagg aaccgtcagc ccattccccg gaggcaaaca tgggctggca aaagtgtagc
      181 ggtattaggc acagctattt aggaaagagg gagcatctcc atctcattaa atggaccacg
      241 ccagacaatt cactgtcatg ctgatgctgc actgtgggga cagaatcact catgatgact
      301 tcgataatcg acgacattat tatttggccg agcttttagg tcatatctca aatcgtttgc
      361 ctaatattgc tccatttttt aataaaaaat caatcatttt tgaatctttg cttttcattg
      421 cattatcaag catagtgtcc ccactatttg ctttttgaaa ttttttgttt acatcaacca
      481 atccattatt gataaaatat tctagcactt tatagttggc accatcttta ctaagtaagt
      541 attcaacatc atacagatta caatatctat tgttgttcat tttctccatc tctgcgacat
      601 cttgttttgt aaaattaaat ttagatagta aataaagtgc tatatcagct tttttat
//
LOCUS       DAJTYF010000335          657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTYF010000335 DAJTYF010000000
VERSION     DAJTYF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.342"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(144..>657)
                     /locus_tag="OO590_004354"
     CDS             complement(144..>657)
                     /locus_tag="OO590_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6639438.1"
                     /translation="VEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMIDRKTVEDTLP
                     VITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGKEINQTVGFA
                     PFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATATINGKDVEL
                     LKAGAIEQKW"
BASE COUNT          168 a          162 c          138 g          189 t
ORIGIN      
        1 tctcggcttt gttcagcttt cagcgacacc aaatgctttt ggtgctgtac ctatgttttt
       61 ggcgattcag catattctcg cggcttagat tattatcgcc acccgccagg gcaagttttg
      121 ccttagccgg gggctgagca gcgttaccac ttttgttcaa ttgcccccgc tttcagcaac
      181 tccacatcct tgccgttaat agttgccgtt gcggcaacta ttgtggtcct tacagttgca
      241 caagattttc gctcaaattc cggtttataa agaataagag acgaattact ggtaaatgaa
      301 ccaaataggt aatcagtaaa gctaatattc agccggttta aaccgaaagg cgcgaagcca
      361 accgtttggt taatctcctt accggatggg cgacgaatgc tgaatgtcac gcgaagattg
      421 gccacttcgc ctaatttatt ggtggtggcg aagaggctat ccatgcagga aaaaccttta
      481 tctaaaaaat tatcccgcat cccagaacgg gtaaccgtga tcacgggtaa cgtatcctca
      541 accgtttttc tgtcaatcat gtcttttcga caagtagcca gaaagtcttc cgggccacca
      601 taaatgtgga cacctttatc aaggtattcc ttcgctttct tacaatctac ctcaaca
//
LOCUS       DAJTYF010000336          655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTYF010000336 DAJTYF010000000
VERSION     DAJTYF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.343"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
BASE COUNT          178 a          178 c          155 g          144 t
ORIGIN      
        1 tgatggcaaa aacagcagac agtatcagta cgataaatgt ggccccccat cccagataat
       61 cgacaacaag tcccattccc agtgtcgcaa caattgagcc gaagatatag gaaaatgtgc
      121 ctgacagccc taacagaata agtttacgcg taaatcgccc ggaatccaga agagcattaa
      181 gtatcacata aacaggttcc ataccagtcc tgaccgcatc aggacgccag taaataaaat
      241 ctcgtagtcc accggcatca gagacaagag taatcgtcgt taccggtatt tcgactggtt
      301 tgtactggtg tgagcggaca aaactactgt acgtctctca tacacgctcg taagcggccc
      361 tcacggcccg ctaacgcgga gatacgcccc gactgcgggt aaacccttgt cgggaccact
      421 ccgaccgcgc acagaggcat tttcatcgct gacaggccgc atggcctcgc agagccggag
      481 ggggactggt gatattcgga cgaccgtacc ggcttgcgcc gggcttcggc ggttttacac
      541 ctgtgctaca tgtaaccacc gtatgccgcc ttatatgcgc tgactcgcat tatttgcctt
      601 gttccgtctg aaagtaatca ctatgattaa atatgattaa caactaatcg ggtat
//
LOCUS       DAJTYF010000337          615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTYF010000337 DAJTYF010000000
VERSION     DAJTYF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.344"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(14..>615)
                     /gene="ospZ"
                     /locus_tag="OO590_004355"
     CDS             complement(14..>615)
                     /gene="ospZ"
                     /locus_tag="OO590_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR6639439.1"
                     /translation="HGYFNESPNIYDKKYISGITRSMAQLKIEEFINEKSRRLNYMKT
                     MYSPCPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEISC
                     KIFSKENSDFLYIVVVFRNDSPQGELRANRFIELYDIKREIMQVLRDESPELKSIKSE
                     IIIAREMGELFSYASEEIDSYIKQMNDRLSQIKARMPVT"
BASE COUNT          186 a          124 c           91 g          214 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaactctgg cgactcatct cttaacactt
      181 gcataatctc tctttttata tcatatagtt ctataaaccg atttgcccgt agttctcctt
      241 ggggagaatc atttctgaat acaacaacta tatacaaaaa atcactattt tctttagaga
      301 agatcttaca actaatctct gggagctcac ataatgcatg taagtcagga tggtacaaac
      361 tatccacaga aaattgcccc ataggacgtt ttccagatat aactgtcaac cagcttcctt
      421 ccggtgtact ggcttcgtct ctggaaatag gctgaaaatc ttccggacat ggagaataca
      481 ttgtcttcat ataattaagt cgtctggatt tctcattaat aaactcctct atttttaatt
      541 gtgccatact tctggttata ccagagatat acttcttgtc atatatgttt ggtgattcat
      601 taaagtaacc atgtt
//
LOCUS       DAJTYF010000338          612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTYF010000338 DAJTYF010000000
VERSION     DAJTYF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.345"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
BASE COUNT          221 a          108 c          128 g          155 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catattaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg gggggggatg aaaatacctg aagcagtaaa tcatattaat
      541 gttcagaata atattgatct tgttgatgga aaaacgaatc cgaataaagc aacaaaagca
      601 ttacaaaaaa at
//
LOCUS       DAJTYF010000339          605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTYF010000339 DAJTYF010000000
VERSION     DAJTYF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.346"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..175)
                     /locus_tag="OO590_004356"
     CDS             complement(<1..175)
                     /locus_tag="OO590_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR6639440.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKI"
     gene            complement(196..>605)
                     /locus_tag="OO590_004357"
     CDS             complement(196..>605)
                     /locus_tag="OO590_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6639441.1"
                     /translation="TMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGPHYK
                     VDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNV
                     YGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
BASE COUNT          134 a          146 c          133 g          192 t
ORIGIN      
        1 gtattttaac tttgctgacc tgcacgccgt tgtcgatcca gcgtttgatc tcgcggatcc
       61 ggtcaatatc ggcatcgttg aacagccgat gaccgccgtc tgtccgttgc ggtttcagca
      121 atccgtaacg cctctgccac gcgcgtaacg tgacagggat taaaggtaaa ccaattgatt
      181 ttgcaagttt ttattttacc ccgctttctt atggggcatg catgggacac tttcagatag
      241 tcttctgtta agaagttcta tctgttcgtg attgttgtct ttcatccatg ctccgtaaac
      301 attgaatacc atttgtgcgt ttgtgtggcc catctggctt gcgataaaac taggattagc
      361 tcctgcggca agtgaccagc atgcataggt atgcctggat tgatacgatt ttctgtgtct
      421 cagacctgcg cgttttaaga tacttgtcca tgactccctg atggagtcaa ccttatagtg
      481 cggtccggaa aactgccgct gttttattac ctgaggacta aaaacaaaag tgcatttatg
      541 cacagtactt ctcccatatt ccctctgctt tacctctaca gaatgctgtt ttccaagcat
      601 ggtca
//
LOCUS       DAJTYF010000340          593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTYF010000340 DAJTYF010000000
VERSION     DAJTYF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.347"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..212
                     /locus_tag="OO590_004358"
     CDS             <1..212
                     /locus_tag="OO590_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639442.1"
                     /translation="IALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGVGPGT
                     TLGLGSLITSLLSIAVIAIAGNNKK"
BASE COUNT          172 a          132 c          107 g          182 t
ORIGIN      
        1 ttatcgcatt acaaataaat ggttcaaaaa caggatttgc cgcatctatt acaggtttta
       61 ctcaaacaat aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc gacaacggtg
      121 ttggtcccgg gactacttta ggtcttggtt cactaataac ttcattactg tcaatagccg
      181 taatcgccat tgcaggaaat aataaaaagt aagtgtactc tgactgcgta tgtatctact
      241 ttgtgtaatg caggtgtcta ttgaaaataa ttcttttcgt gggcatctgt tgtataaaat
      301 aaaaaattat cgtaagtaca tctatctgcg atgtcttgtg aaaattatcc tgtctggcaa
      361 caatcgcgtc catctgtatt aatggacgcg aacgatgaac tctcaaacaa cactagatat
      421 caccggcttt cgcacttatt tgccagaatc attacgtctc agattcgacg agcaattatc
      481 tgtccgggct atcgcacagc agttaagact cagttattcc cccattcaca atctctttca
      541 acgaattaac gcctcaggta ttgtctgacc atgacccgag tccctttcat tcg
//
LOCUS       DAJTYF010000341          591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTYF010000341 DAJTYF010000000
VERSION     DAJTYF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.348"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..147
                     /locus_tag="OO590_004359"
                     /pseudo
     CDS             1..147
                     /locus_tag="OO590_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
     gene            144..>591
                     /locus_tag="OO590_004360"
     CDS             144..>591
                     /locus_tag="OO590_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR6639443.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIATSPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALA"
BASE COUNT          132 a          143 c          172 g          144 t
ORIGIN      
        1 gactagtgca gtgaagagca gcatccggtg atctggtcaa ccacgcaggc tgcagaacac
       61 ttaccggttc gcatgcctcg cctctgctct gcagaacgcc tctctgaaag tatggtcgat
      121 gaaatatttc agaagggggc tgcatgagta cagaaatgaa aacggggctg gtgctgtccg
      181 gtggcggggc ggtgggcgct tatcaggcgg gagtggttaa ggcactggca gagtgtgata
      241 cacagatcag catggtttca ggggccagca ttggcgcatt caatggtgcc attatcgcga
      301 cctctcccga tctgtcagaa gctgccgtac gcctggaagc gctctgggat catctgggga
      361 ataatcaggt gctgtcggta aaccgattgg tttacttttc attgctgaaa aaattgttcc
      421 aggcaatgaa cctctgccag atccccggac gtgcaggagc actgcttacg acgcttcttc
      481 gccatatatc gatactcaac gggtttgaca atctgatggc tcagccgttg ttgtcagatg
      541 agcccctgac agcgctgttg gatcattatc ttgatactga tgctctggca g
//
LOCUS       DAJTYF010000342          590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTYF010000342 DAJTYF010000000
VERSION     DAJTYF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.349"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            23..>590
                     /locus_tag="OO590_004361"
     CDS             23..>590
                     /locus_tag="OO590_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6639444.1"
                     /translation="MFTANQCWTSFLDAGGLRDIEVEAVTKMLACGTRILGVKEYICD
                     KPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTLWPVF
                     ESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLN
                     KHGQWKKLSFLKDAMRSRWMWNMRQRLLK"
BASE COUNT          124 a          166 c          178 g          122 t
ORIGIN      
        1 tactccccgc ccgctgaaac gcctgttcac agccaaccag tgctggacgt ccttcctgga
       61 tgcgggcggt ctgcgcgata tcgaggttga ggctgtcacc aaaatgctgg cctgcggcac
      121 gcggatactg ggcgtgaagg aatacatctg cgataaacct gagtgccccc acgtcagata
      181 cgtcactaac tcatgcggca gccgtgcctg cccgtcctgc ggaaaaaagg ccacagacct
      241 gtggatagcg acacagctga atcgtcttcc tgactgcgac tgggtacacc tggtcttcac
      301 cctgccggac acgctgtggc cggtgttcga aagcaaccgg tggctgctga atgacgtgtg
      361 ccgtctggcg gtggagaatc tgctgtatgc cgcccggaaa cgggggcagg aacccggtat
      421 cttctgcgcc atccacacgt atggccgtcg tctcaactgg catccgcatg tacatgtgtc
      481 tgtaacctgt ggaggtctga ataagcatgg tcagtggaaa aagctgagct tcctgaaaga
      541 cgcgatgcgt tcgcggtgga tgtggaatat gcggcaacgg cttctgaaag
//
LOCUS       DAJTYF010000343          582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTYF010000343 DAJTYF010000000
VERSION     DAJTYF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.350"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..535)
                     /locus_tag="OO590_004362"
     CDS             complement(<1..535)
                     /locus_tag="OO590_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6639445.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPG"
BASE COUNT          165 a          142 c          124 g          151 t
ORIGIN      
        1 agcctggacg ctctgtgatc cagtcttcca gtacacattc taaacgacca gtatcaaggt
       61 actccttgat ctgttcatag agcagatacc cgataccagc acccatcaaa acggcctcca
      121 gttcagcatc aacatcatcc agaatgatat ttcctgcgac agcaatttcc agctcctttg
      181 caaattgcca gtggaaaggt ttgccgctgg gataacgaaa aacaacacat tgatgattaa
      241 gcagatcatg aggatggcgg ggtttgccat atcttgcaaa gtactctggc gttgctgcta
      301 cacataattt cactggtggg ccgatcgcca cgctgatcat atctttttcg acgatgccac
      361 taagtcgaac gccagcgtca aagccctgtt ggacgatatc gataagagaa tcgtcagttg
      421 tgagctctac tttaatatca ggatattcac gtgtgaaacc aaccagtaaa ggcattaaaa
      481 atatacgtgc agctacccgg gcggcattga ttttgagcgt tcctgtcggt gtcagacgaa
      541 aatcattcat ctcatctaac atgatctgaa tttcgtcgaa ag
//
LOCUS       DAJTYF010000344          568 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTYF010000344 DAJTYF010000000
VERSION     DAJTYF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.351"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..297
                     /locus_tag="OO590_004363"
     CDS             <1..297
                     /locus_tag="OO590_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR6639446.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>568)
                     /locus_tag="OO590_004364"
     CDS             complement(405..>568)
                     /locus_tag="OO590_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639447.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          105 g          145 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaatg
//
LOCUS       DAJTYF010000345          552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTYF010000345 DAJTYF010000000
VERSION     DAJTYF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.352"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>552
                     /locus_tag="OO590_004365"
     CDS             <1..>552
                     /locus_tag="OO590_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vimentin yjdA"
                     /protein_id="HCR6639448.1"
                     /translation="EAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINTRLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRRRQRGLWGKICGAFGTSDWGW
                     ETYKEDVSRSVININTVRKEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQER"
BASE COUNT          150 a          129 c          161 g          112 t
ORIGIN      
        1 gaggctgaag cacagttccg gcctgagctg gagaaaatcg tcagcgggat tgaaacaggt
       61 tttcgtggca cggccctgta cgccacagaa aacattgccg gtcgcatcaa tacccgcctg
      121 gaggatgagg gctttaccgt aaaaatcagt tttccggcag tcagtcagtt acaaacccgg
      181 ctcgcggtaa aaacaaatct gagtgcgctt atggaggaaa gaacggagac cgtcacccgt
      241 cgccgtcggc agcgtggttt atggggaaag atttgtggag cgtttggcac cagtgactgg
      301 ggctgggaaa cctataaaga ggatgtgagc cgcagtgtta tcaatatcaa caccgtcagg
      361 aaggaagtta tgtcactgac ccgggcatat ttcggggagc tgcaggcatc cattgagcag
      421 gatattaacc agcccgtccg ccaggaaatc gatgcctttt tctgcgcatt cagggagaaa
      481 gttgaacaac tgcgtaacac gctgattcag agctctgaag atcataaacg cgatcagcag
      541 gcacaggaac gg
//
LOCUS       DAJTYF010000346          544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTYF010000346 DAJTYF010000000
VERSION     DAJTYF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.353"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>544
                     /locus_tag="OO590_004366"
     CDS             <1..>544
                     /locus_tag="OO590_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6639449.1"
                     /translation="VGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSS
                     TDDAAAINALRQQIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVT
                     LFGLISHPGNQPFTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRH
                     VEPMPGSIIYVGLADSVWSET"
BASE COUNT          117 a          161 c          166 g          100 t
ORIGIN      
        1 ggtagggcaa cctcgactgg caaacagctg gtggcctggt gcggtgatta gcgaagagct
       61 ggcaacggcg gcagcattgc gtcagcagca ggcgttgctg acacggctgg cagagcaggg
      121 cgcagattcc agcaccgacg atgccgccgc cattaacgcc ttacgccagc aaatccaggc
      181 attgaaagtg acaggcaggc aaaaaatcaa tcttgatccc gatatcgtcc gcgttgccga
      241 acgcggtaac ccgccgttgc agggcaacta cacgctgtgg gtcggaccac cgccgtccac
      301 ggtcacgttg ttcgggctta tcagccatcc tggcaatcag ccattcactc caggtcgcga
      361 cgtggcgagc tatctctctg accagagcct gctcagcggt gcggatcgca gctacgcgtg
      421 ggtcgtttac ccggacggac gcacgcaaaa agcgccggtg gcttactgga acaagcgtca
      481 cgtagagccg atgcccggca gcattattta tgttggcctc gcagattccg tctggagtga
      541 gact
//
LOCUS       DAJTYF010000347          541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTYF010000347 DAJTYF010000000
VERSION     DAJTYF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.354"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(291..>541)
                     /locus_tag="OO590_004367"
     CDS             complement(291..>541)
                     /locus_tag="OO590_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6639450.1"
                     /translation="PQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALI
                     ARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT          162 a          113 c          132 g          134 t
ORIGIN      
        1 aaacggtatt caagtatcag aaaaccttag ttcaggatcg cactagctgc atgatcgaaa
       61 tctcattctg cgaacggtca ggcattttag aaaagggaat taaagggata aataacggcg
      121 aaatagagtg atcaaataac gtctgtttat tagccagcca gcgacatttg gttcgcgatt
      181 agcgaagaaa aatcatcata aattggtaaa aaatataaca ggtagttaaa aaacatcagt
      241 gctgggtaaa ttgccggatg acatcagaac gatgccatcc gaacggtggc ttaaccctga
      301 cggttgaaac gttgcgtttt aacgtccagc gttagcgttt cttctggttg tatcgcatac
      361 agtttctcac cctgctggca aatccagcca atccccagtt cacgggcgat tagggcgctg
      421 tgggataccg gacttccggc gctaaggcag atacctttta caaccgccgg atccagttgc
      481 agtactgtgg aaggataaat gttctccgcc agtagaatag ttggcgagtt aaactgcggg
      541 a
//
LOCUS       DAJTYF010000348          540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTYF010000348 DAJTYF010000000
VERSION     DAJTYF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.355"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..221)
                     /locus_tag="OO590_004368"
     CDS             complement(<1..221)
                     /locus_tag="OO590_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6639451.1"
                     /translation="MAHIQLVKQTSSGLLLPATSESCEFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYRTPVGGAITPRER"
     gene            complement(288..>540)
                     /locus_tag="OO590_004369"
     CDS             complement(288..>540)
                     /locus_tag="OO590_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639452.1"
                     /translation="EELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAALIH
                     LVDGLVTDGFLLCGDEFVTSLRALKETGLKAGFSAFECE"
BASE COUNT          148 a          142 c          117 g          133 t
ORIGIN      
        1 cgttctcgag gcgtgatcgc cccaccgacc ggagtccggt aatcgaatcc cagttgcagg
       61 agtttgaaaa aacgcttgtg gaatgcgtag ttacgaacgc gcttaaaatc agcgtgtatc
      121 cactcgcctg ttttgatttg atgcagaaat tcgcaactct ccgacgtcgc cgggagaagt
      181 aatccggaag aggtttgttt gaccagttgt atatgtgcca tcggctttct ccggtggcac
      241 ggtgttactc agcagggggt caaccctgca ctgaattgta gatgaactca ctcacattca
      301 aaagcagaaa agccagcctt aagtccagtc tctttcagcg cccgtaatga cgtgacaaat
      361 tcatctccac acaaaagaaa accatccgtc acaagcccat ccaccaaatg aattaacgca
      421 gctccactct tcctttgttg agactgtaaa catttaatac ggcagtggct gacaatagcg
      481 ccattctcaa cgcgcacagt atagaggcca tcttcactaa aaatttcacg taattcttcg
//
LOCUS       DAJTYF010000349          531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTYF010000349 DAJTYF010000000
VERSION     DAJTYF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.356"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            7..513
                     /locus_tag="OO590_004370"
     CDS             7..513
                     /locus_tag="OO590_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6639453.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          143 a          119 c          127 g          142 t
ORIGIN      
        1 tcacagattc ctgttattac caccgaactt ttggcgcagc tttacggcac tgagccggtg
       61 cgtattcgcc agaatcatca tgagaacaaa gtacgcttcg ttgaagggaa acactttttc
      121 aaagttgttg gtaatgacct taaagaattg cgggtcgctt taaactactc acaaaatcca
      181 gtttcaccca aagcccgctc cctcatcctc tggacagaac gaggcgcagc acgccacgcc
      241 aaaatgctcg aaaccgatca ggcgtgggat gtgttcgaaa aactggaaga ctgctatttc
      301 agtcaaaggg atccgtcaac gccagtttca tgccagaaaa gttacgacac gcgagttctc
      361 tgttatcaga gaggcggtgt cactgtttcc acaattcagt tacgggatga tgatattgtt
      421 atttcccttg agtcatggct ggaactggcg agagccaatg gttggtttgt tgttcgcaga
      481 gataaactgg tggaaaggtt gatgcagctt taaaaaagtt cttgcatttt t
//
LOCUS       DAJTYF010000350          528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTYF010000350 DAJTYF010000000
VERSION     DAJTYF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.357"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>528
                     /locus_tag="OO590_004371"
     CDS             <1..>528
                     /locus_tag="OO590_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6639454.1"
                     /translation="FDDDLAAQTTQASNRIRGLLTQIHPALERVLGPRLEHPAVLDLL
                     QRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLP
                     RLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFAS
                     AAHLAAYAGLAPVTR"
BASE COUNT          103 a          180 c          141 g          104 t
ORIGIN      
        1 gcttcgatga tgatcttgcc gcacagacaa cgcaggccag caaccgtatc cgcggccttc
       61 tgacccagat acatccggca ctggagcgcg ttctcggtcc gagacttgag cacccggcgg
      121 tactcgatct tctccagcga tatccctcac cagaaaaact cgcttcgctg ggtgagaaga
      181 agctggcagc ccagctctgc aaacttgcgc ctcgtctggg taaacgcctt gcagcagaca
      241 tagctcaggc actggccgaa caaaccgtcg tcgttcccgg cacgaatgcc gctgccgtag
      301 tactgccacg tctggcactc cagctcatca cgctgcgtaa gcaaagagac gaggtggcgc
      361 ttgaggtaga acagcgagtt cttgctcacc ctctttaccc ggtcctgacc agtatgcccg
      421 gagtcggtgt caggaccgca gccagactcc tcaccgaggt cgcctgccgc gccttcgcct
      481 ctgccgcaca tctcgctgct tatgctggcc ttgcgccggt aactcggc
//
LOCUS       DAJTYF010000351          523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTYF010000351 DAJTYF010000000
VERSION     DAJTYF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.358"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..201
                     /locus_tag="OO590_004372"
     CDS             <1..201
                     /locus_tag="OO590_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6639455.1"
                     /translation="LEYTEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            211..>523
                     /locus_tag="OO590_004373"
     CDS             211..>523
                     /locus_tag="OO590_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6639456.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NF"
BASE COUNT          118 a          138 c          152 g          115 t
ORIGIN      
        1 ctggagtaca ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc cctgcgtacc
       61 cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt gatcggcctg
      121 caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg gctgtgccgt
      181 attccggaaa aacgttctta aggagggaag atgcgccatt gtgggtggtt gctgggattg
      241 ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa aaatgaggcc
      301 aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa ccgctatctc
      361 gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat tgttcgtctc
      421 gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg tcgtaaaacg
      481 ggcggctcgg tggctctgct ctgggtgaac ggcgaaaact tcc
//
LOCUS       DAJTYF010000352          505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTYF010000352 DAJTYF010000000
VERSION     DAJTYF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.359"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..505
                     /locus_tag="OO590_004374"
                     /pseudo
     CDS             1..505
                     /locus_tag="OO590_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          147 a          120 c          137 g          101 t
ORIGIN      
        1 gaagctcgcc ttgagcgaga tatattaaaa aaagcaacag cgtattttgc acaggagtcg
       61 ctgaaaaata cgcgttaatc gaacaatggc gacaacaatt tcccattgaa gcgatgtgtc
      121 aggtatttgg tgtatccagg agcggttatt acaactgggt acagcatgaa ccctcagaca
      181 gaaaacaaag tgatgagcgg ctaaaactgg agattaaggt ggcacatatc cgcactcgcg
      241 aaacatatgg aacccggcgg ctccagacgg agctggcaga gaatggcatc atcgttggtc
      301 gtgaccgact ggcacgtctt cgtaaggagc tgaggctacg ctgtaagcag aaacgcaagt
      361 tcagagcgac tacgaacccg aaccacaatc tgccagttgc gccaaatctg ctgaaccaga
      421 cgttcgctcc tacagcacca aatcaggtct gggtggcgga cctgacgtat gttgccacac
      481 aggagggatg gttgtacctc gctgg
//
LOCUS       DAJTYF010000353          501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTYF010000353 DAJTYF010000000
VERSION     DAJTYF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.360"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..258
                     /locus_tag="OO590_004375"
     CDS             <1..258
                     /locus_tag="OO590_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639457.1"
                     /translation="YNGEDRGVFMMAKRFTALSFGCFRHDGESITVVLSRDGDSQSHD
                     LEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
BASE COUNT          125 a          113 c          128 g          135 t
ORIGIN      
        1 tacaacggtg aagaccgtgg cgtgttcatg atggctaagc gttttacggc tctgagcttc
       61 ggttgcttcc gtcacgatgg cgagagcatt actgtggtcc tttcacgtga tggagactcc
      121 cagtctcacg accttgagat tctcggcaag gtgtttgttg tagctgaagc tgctacgacc
      181 atcaagatgg gtactggctc ggctactgct gtcctgcctg tagtcaaacg cctgagcttc
      241 actaaagaag ctaactaaca ataacgacta ttggtcagac ttccgaactg gatgtctggc
      301 ctttattttg tactagtaat ctagttcgtg aatacagaaa gtaggagggc caaatggacc
      361 tacacaaagt ctatctcgtg gtcttcctgc taaagctggg actggtggtc tggctatggg
      421 ctattagacg atgagttttg actgaaaaat gtgagacctc atctaatata aagagcgagc
      481 caaaagcccc catagcctga c
//
LOCUS       DAJTYF010000354          483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTYF010000354 DAJTYF010000000
VERSION     DAJTYF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.361"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
BASE COUNT          139 a           96 c           79 g          169 t
ORIGIN      
        1 cagagcaaaa aagaagctca gcaatccaca ctctccgtcg aatcaccggt acaacgttag
       61 cggttacctt ctccactttc acagaacata acggcacttc gctgtcggat gcttttgctc
      121 tttgggatta tcaaagcggc agatattctt tcatcttaaa ttttacgtct ttgtcctgac
      181 tgatgtttat cctgtttggc tgcgaaataa atataaaatt aatatatatg ttgtaatgat
      241 atatttttat aaattattcc ctgcgtgaat tttaataaat ttaatctatc cctttatacg
      301 caatacattt actttcctct tttgatgatc ttaaatgtct tatttttcgt aatgtgtata
      361 acaaggaata gtgatgaaat ttaaaaaatg tcttctgcct gtggcaatgt tagcgtcatt
      421 cactctggca ggatgccagt caaatgctga cgatcatgcc gccgatgttt atcaaaccga
      481 tca
//
LOCUS       DAJTYF010000355          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTYF010000355 DAJTYF010000000
VERSION     DAJTYF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.362"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..248)
                     /locus_tag="OO590_004376"
     CDS             complement(<1..248)
                     /locus_tag="OO590_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6639458.1"
                     /translation="MHEKNITLLCDEADRLLQLSINLLRQMVDEPDVLSDGKNENGQL
                     FDKQKALKRIKELEGEQIKTARREMVLAVVGTMKAGKST"
BASE COUNT          126 a          116 c           97 g          137 t
ORIGIN      
        1 gttgatttgc ctgctttcat cgtgccgaca acagccagca ccatctccct gcgggcggtt
       61 ttgatttgtt cgccctccag ctccttaatt cttttcagtg ctttctgttt atcaaaaagc
      121 tgcccgtttt cgttcttacc gtcagataac acatccggct catcaaccat ttgccggagc
      181 agattgatgc tcagttgcaa aagccggtcg gcttcatcac aaagcagggt gatgttcttt
      241 tcgtgcatta tttaaccttc ctgagacgca ttttcatgac aaccacggag gaggccattt
      301 actacaagcg atcgcttata acgatcaata taatagaaat gatcgtcata ttctattaat
      361 atgaaaaaca caacgcaagg gggacaaccc gggtatggct gcgtttgcat tgtgttttca
      421 tagagttaaa aatctgtaac gacaacccgg cacatcacta ccgggttaag aagaaa
//
LOCUS       DAJTYF010000356          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTYF010000356 DAJTYF010000000
VERSION     DAJTYF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.363"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            268..>476
                     /locus_tag="OO590_004377"
     CDS             268..>476
                     /locus_tag="OO590_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6639459.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAV"
BASE COUNT          152 a           66 c           94 g          164 t
ORIGIN      
        1 taaattttgt gtatcttttt ctgaaaataa actaaagaaa gggctttata tatcacgcat
       61 atttatttgt tggatagtca ttagatgttg aatcaatgaa ttactcgttc agttaatatt
      121 taaatattca tgaaatctat aaattaaaga tttgtcactt attggattta gtatgcttga
      181 tttaaatggc ggtcaattat tgttattatt aagtctaatg tcatttaaag gcattatcat
      241 atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc gtttataact
      301 gtggtatgtg caacgtccag cgttatggct gctgatgata atgctatcac ggatggctca
      361 gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc gacgaaagat
      421 tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg agcagt
//
LOCUS       DAJTYF010000357          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTYF010000357 DAJTYF010000000
VERSION     DAJTYF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.364"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..437
                     /gene="glyS"
                     /locus_tag="OO590_004378"
     CDS             <1..437
                     /gene="glyS"
                     /locus_tag="OO590_004378"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="glycine--tRNA ligase beta chain; glyS; class II
                     aminoacyl tRNA synthetase; tetramer of alpha(2)beta(2);
                     catalyzes a two-step reaction; first charging a glycine
                     molecule by linking the carboxyl group to the
                     alpha-phosphate of ATP; second by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6639460.1"
                     /translation="IQAVLARRPTRPADFDARMKAVSHFRTLEAAAALAAANKRVSNI
                     LAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDKLEPYFAEGRYQDALVELAELREP
                     VDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRVADISLLQ"
BASE COUNT          107 a          117 c          140 g          108 t
ORIGIN      
        1 ccatccaggc ggtactggcg cgtcgtccga ctcgtccggc tgatttcgat gcccgcatga
       61 aagcggtatc gcacttccgt accctggaag cagctgcagc actggcggcg gcgaacaaac
      121 gtgtatctaa catcctggcg aaatctgacg aagtgctgag cgatcgcgtg aatgcctcta
      181 ccctgaaaga gccggaagaa attaaactgg cgatgcaggt tgtggtgcta cgtgacaagc
      241 tggagccgta ctttgcggaa ggtcgttacc aggatgcgct ggtcgaactg gcagagctgc
      301 gtgagccggt tgatgctttc ttcgataaag tgatggtcat ggttgatgac aaagaattgc
      361 gtctcaaccg tctgaccatg ctggagaaac tgcgcgaact gttcctgcgc gttgcggata
      421 tttcgctgtt gcagtaataa cgccgttatt aaaaagcctg ccatctggca gg
//
LOCUS       DAJTYF010000358          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTYF010000358 DAJTYF010000000
VERSION     DAJTYF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.365"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..194
                     /locus_tag="OO590_004379"
     CDS             <1..194
                     /locus_tag="OO590_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6639461.1"
                     /translation="KNYLFFGSDNGGESAAIIYSLLVTCKLNGVEPEDWLREVIVKLN
                     DWPSNRVHELLPWNFSAVK"
BASE COUNT          114 a          103 c          121 g          132 t
ORIGIN      
        1 gaaaaaatta tctcttcttc ggttctgaca acggtggaga aagcgcggcg attatctaca
       61 gtctgctggt cacctgcaaa ctgaacggag tggagccgga ggactggttg cgcgaagtga
      121 tcgtgaagct caatgactgg ccgtcgaacc gagtgcatga actgctgccc tggaacttct
      181 cagctgtaaa ataatccttc cgctacgtac ttctctgggc gcttaccact caacggcatc
      241 gtcaaaatta tcaaagcgca gaacatagat ttatcatgtg ttttatggat ggctttctcc
      301 atcaggcatc gttcgtcacg ggaaattggt gctatgatcg gcattgatca gtccggttgg
      361 tgatttgttt tgatttggcg attgatcaga tcgcacaatc cgggctgagt tccctctaag
      421 tgatctaggt tgtgtccctt aattacgtaa gcgttgataa aagagtcgga
//
LOCUS       DAJTYF010000359          468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTYF010000359 DAJTYF010000000
VERSION     DAJTYF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.366"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            187..>468
                     /locus_tag="OO590_004380"
     CDS             187..>468
                     /locus_tag="OO590_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6639462.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELSILQKAATYF"
BASE COUNT          146 a          118 c          106 g           98 t
ORIGIN      
        1 agaaatataa cattccagtt ataatattca ataatcaatg cgccgccgag aggtgctact
       61 aatggtatta ctgcgcttac agatgatata tcagaaagcg cccgttttag ttttaatgga
      121 gggtgatctt acccaccaat agtggacaca ggactaagtg agtaaactct caaccagagg
      181 tgactcatga caaaaccagt atcaatcagc aagaagcccc gtaaacaaca tacgcctgaa
      241 tttcgtaacg aagccctgaa actcgctgaa cgcatcggtg tggccgccgc agcccgtgaa
      301 ctcagcctgt atgaatctca gctttatgcc tggcgcagta aacagcagca acaaatgagt
      361 tcgtcagagc gcgaaagcga actggccgct gaaaatgtcc gccttaaacg acaactggcg
      421 gagcaggctg aggaactgtc catcctccaa aaggccgcga catacttc
//
LOCUS       DAJTYF010000360          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTYF010000360 DAJTYF010000000
VERSION     DAJTYF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.367"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>464
                     /locus_tag="OO590_004381"
     CDS             <1..>464
                     /locus_tag="OO590_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6639463.1"
                     /translation="QHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
                     RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          113 a          111 c          133 g          107 t
ORIGIN      
        1 ctcaacacgg ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acattggggc tacgtcggtg
      121 ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg
      181 cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg
      241 cctttgaggt cgtggtatgg atgacggatg gctggccgct gtatgaatca cgcctgaagg
      301 gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc
      361 tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DAJTYF010000361          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTYF010000361 DAJTYF010000000
VERSION     DAJTYF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.368"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(107..>463)
                     /locus_tag="OO590_004382"
     CDS             complement(107..>463)
                     /locus_tag="OO590_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6639464.1"
                     /translation="PAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTA
                     GAVGGGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTT
                     YNETRIQPNTKCPEKS"
BASE COUNT          106 a          120 c          111 g          126 t
ORIGIN      
        1 ctgcgacagc agagagttga tcctgcaatg atgaggctat agccggatag cttaaggagg
       61 ctaagatggc gcaaagaaaa actttcttca tgactcctcc tgattattag ctcttttcag
      121 gacatttggt atttggctga atacgcgttt cgttatacgt tgtggtaata acaacggcta
      181 aacctgtcgt aaactggcac tctttaccct cctgggtgga ggtatacact ttggtgcctt
      241 ccttatatgt taaagaaaca ccttccacta aggttttatc attcaccata gaacccgctg
      301 ccgcgcctac agctccgccg ccaactgccc ctgccgtcgt tccggaattg ctgccagaac
      361 cgacgttgtg gccgataaca ccgccagcga ctgcgccaat aagcgcgccg aaggcttgtg
      421 cgttccgttt attttgggag ttgtctacgg caacttttgc ggg
//
LOCUS       DAJTYF010000362          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTYF010000362 DAJTYF010000000
VERSION     DAJTYF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.369"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>450
                     /locus_tag="OO590_004383"
     CDS             <1..>450
                     /locus_tag="OO590_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6639465.1"
                     /translation="PESLSHITTESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLG
                     RYLKKPPIAASRLAHYNGGASLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFK
                     MVRYFGFLASRVCGEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSR"
BASE COUNT          116 a          104 c          150 g           80 t
ORIGIN      
        1 gccggagtcg ttgtcacata tcacgacgga atcacagagg agaagcctgg tgctgaaagc
       61 cggaggaaaa tactggcatg tgtacatgtc gaaaaaaacg gccggcggga ggaatacggc
      121 gcgctacctg ggtcgttatc tgaagaagcc gccgatagcg gcctcccggc tggcacatta
      181 caacggaggg gcgagcctga gcttccgtta cctggaccac aaaacgggag aaacggcgac
      241 ggaaacgctg acacagcgtg agctggtcgc gaggctgaaa cagcacatcc cggagaagtt
      301 ttttaagatg gtgaggtact tcgggttcct tgccagccgg gtgtgtggag agaagctgcc
      361 gcaggtgtac cgtgctctgg ggatggataa accggaacca gtggcgaaag tgtgctatgc
      421 acaaatggtg aaacagttcc tgagtcgtga
//
LOCUS       DAJTYF010000363          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTYF010000363 DAJTYF010000000
VERSION     DAJTYF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.370"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            71..439
                     /locus_tag="OO590_004384"
                     /pseudo
     CDS             71..439
                     /locus_tag="OO590_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          124 a          100 c          119 g           96 t
ORIGIN      
        1 cagacaacca gtatcactta tttaagtgat agtcttaata ctagttttta gactagtcat
       61 tggagaacag atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga
      121 aaagatcgca attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg
      181 gcaacatggt gtagcagcca gccagttatt tctctggcgt aagcaatacc aggaaggaag
      241 tcttactgct gtggccgccg gagaacaggt tgttcctgcc tctgaacttg ctgccgccat
      301 gaagcagatt aaagaactcc agcgcctgct cggcaagaaa acgatggaaa atgaactcct
      361 caaagaagcc gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc
      421 cggggatggg gagtaagct
//
LOCUS       DAJTYF010000364          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTYF010000364 DAJTYF010000000
VERSION     DAJTYF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.371"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            211..>439
                     /locus_tag="OO590_004385"
     CDS             211..>439
                     /locus_tag="OO590_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6639466.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYD"
BASE COUNT          138 a          106 c           61 g          134 t
ORIGIN      
        1 tcgaacctcg cttaccaacg cgttgtcagt catctccccc ctccctggtg catcgttata
       61 tccgtgatga gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca
      121 gaaaacaaac ttatttttat taaaatcaat tctttttctt tgaacattca actgcgatat
      181 ttgtaaaaac attaaacagg caacaataaa atgacattac caacaactat ttattcattc
      241 cccgcatatt taccccgttt ttcgtccacc gacaaaccag caaaattaaa atttcatcaa
      301 tacgcacgcg ctacattact gtcaaatcgt ggaagagatc ataattgcga tggcagacgt
      361 actgttgaga tccacaaact agatctctct gactggcaag cattcaataa gttagcaacg
      421 aagtgtaatg cttacgatg
//
LOCUS       DAJTYF010000365          438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTYF010000365 DAJTYF010000000
VERSION     DAJTYF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.372"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..>438)
                     /locus_tag="OO590_004386"
     CDS             complement(<1..>438)
                     /locus_tag="OO590_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HCR6639467.1"
                     /translation="WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASA
                     GEAGFLPPHHYDQIFTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSF
                     WFTVVGVILVNVSLGVGEFAQTGWLAYPPLSGIEYSPGVGVDY"
BASE COUNT          125 a          128 c          113 g           72 t
ORIGIN      
        1 cagtaatcaa caccgactcc cggactgtac tctattcccg atagcggtgg ataggccagc
       61 cagccggtct gcgcaaattc gcccacgccg agagaaacgt taaccagaat cacgccaaca
      121 acagtaaacc agaagcttaa gttgttgagg aacgggaacg caacgtcacg cgcgccgatc
      181 tgcagcggaa ccaccaggtt catcagaccg ataacgaaag gcatcgccac gaagaagatc
      241 ataatcacgc cgtgcgcggt gaagatctga tcgtagtggt gaggtggcag gaagcccgct
      301 tcacccgccg aggcaagagc ctgctggcta cgcatcataa tggcgtcagc aaaaccacgc
      361 agcaacatca caatcgccac gatgatatac atgataccga ggcgtttatg gtcgacggag
      421 gtcagccact ctttccac
//
LOCUS       DAJTYF010000366          438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTYF010000366 DAJTYF010000000
VERSION     DAJTYF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.373"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..328
                     /locus_tag="OO590_004387"
     CDS             <1..328
                     /locus_tag="OO590_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6639468.1"
                     /translation="GIDKPELEAFLREMIEGKQKSWLTHCTDAEALKIDSVIGEVLAD
                     HPGLLNVLSQRYVGRGMSKRRMAELLNEQYPEWALITCRRRVDQWLRVAEFILYSPMR
                     EAFDYA"
BASE COUNT          131 a           81 c          119 g          107 t
ORIGIN      
        1 gggaattgat aaaccagagt tggaagcatt tttgcgtgag atgatcgaag gaaagcaaaa
       61 aagctggtta actcactgca ctgacgcaga ggcactaaaa attgatagtg ttataggtga
      121 agttctggcg gatcatccag gactactaaa tgtcctgagt cagcgttatg tggggcgagg
      181 gatgagtaag agaaggatgg ccgagttatt aaacgaacag tacccagagt gggcgttgat
      241 tacatgccga cgtcgtgttg atcagtggtt gcgtgttgct gagttcattt tgtattcacc
      301 gatgagagaa gcgttcgatt atgcttaaaa aatcattgca aaatgagcca caaactgctt
      361 caattcaggt acagcttcgc aaagctgtat cgcgaggcga accaagcgca tgaactttac
      421 cagaacccgc cattgagc
//
LOCUS       DAJTYF010000367          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTYF010000367 DAJTYF010000000
VERSION     DAJTYF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.374"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            90..155
                     /locus_tag="OO590_004388"
                     /pseudo
     CDS             90..155
                     /locus_tag="OO590_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT          138 a           90 c           95 g          108 t
ORIGIN      
        1 ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
       61 actgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
      121 tgctttgtga tattaatcct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac
      181 gaagatcgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg
      241 acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca
      301 taaacaatat caggaattga cactggttat ccatacagat aaaaataaca cctgtacata
      361 actacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac
      421 taaaatgcca a
//
LOCUS       DAJTYF010000368          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTYF010000368 DAJTYF010000000
VERSION     DAJTYF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.375"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..67)
                     /locus_tag="OO590_004389"
     CDS             complement(<1..67)
                     /locus_tag="OO590_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6639469.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVL"
     gene            85..237
                     /locus_tag="OO590_004390"
     CDS             85..237
                     /locus_tag="OO590_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639470.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
BASE COUNT          134 a           68 c           89 g          140 t
ORIGIN      
        1 gcagtacgat acgggtcatg cgactgttaa atcggtgtcg ttttcgcata ctagttgcct
       61 gactcacaaa aggttccttg aagtatgtcc cacgctgtgg acggtactta cattaaggca
      121 caacaggaca aaatggtcaa ttcttcgtta tgtaaaaaag cgtcagtgga tacatatttt
      181 aatgttatgg aagttaattt aattatttac aatgatgatg taataatgat gaattaacat
      241 gataaacgta ataattcatt aatcagggtt attttgtttg atatatatca attggaactt
      301 tcatatgaca cttagaatca atgctctcta tagtgatgaa taatcatcat tcgaagtcag
      361 gtgggatgcc tgtctgaata cacctccttc aggatgtggg ggattctgct gagcatctat
      421 gaaactgaat g
//
LOCUS       DAJTYF010000369          429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTYF010000369 DAJTYF010000000
VERSION     DAJTYF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.376"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(9..218)
                     /locus_tag="OO590_004391"
                     /pseudo
     CDS             complement(9..218)
                     /locus_tag="OO590_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
BASE COUNT          131 a          108 c           79 g          111 t
ORIGIN      
        1 ctcactcctt atttacccgg gaaatacttc tccagcaatg catacaggat ggcagcggtt
       61 tcagcatcca ttatgccgtc ataatttttc tgccggaaat gaagctgaaa agcgcggaca
      121 agcgccttat atccgtcatc cgtctccaca ccagaaatac tgtatccata ggtcttcagc
      181 cgtgacacga tatcaacctt atccggaatg ccctgcgatt caaaaaataa atatttagtc
      241 agtaatttta tgtttttcct gagaaaatta ctctttttcg aactccagtg agcgttcacg
      301 actcatcagt gtgaaaatga tggcaaaaac agcagacagt atcagtacga taaatgtggc
      361 cccccatccc agataatcga caacaagtcc cattcccagt gtcgcaacaa ttgagccgaa
      421 gatatagga
//
LOCUS       DAJTYF010000370          416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTYF010000370 DAJTYF010000000
VERSION     DAJTYF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.377"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..>416)
                     /locus_tag="OO590_004392"
     CDS             complement(<1..>416)
                     /locus_tag="OO590_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6639471.1"
                     /translation="AVGWNWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNG
                     GIFYRSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRT
                     WNGPGWSDRSGYVVTGSVNSNRDELIDTTQARPIQY"
BASE COUNT          117 a          110 c           83 g          106 t
ORIGIN      
        1 caatactgaa ttggccttgc ctgagttgta tcaattagtt catctctgtt tgagttaacg
       61 gaaccggtaa caacataacc tgacctgtca gaccagccgg gaccattcca tgtccgaaca
      121 gatgacagac caccaaggcg aatacctgta ataaaccttg agttacattc tgcctgcgta
      181 tatgcgccaa catctctcgc tgatggcttg cgtgttgtgg tgtaaaactc tgaccagcca
      241 gcctcaaatc cataaccatc acgcgctgaa cgataaaaaa taccgccgtt cttataattc
      301 acacggaact gtacagcagg gcagctcccc gtattcttat tgaagtggag gattaatgtt
      361 gatgcaccgc tgatatttgc atcataaaca ccgctattcc agttccagcc aacagc
//
LOCUS       DAJTYF010000371          415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTYF010000371 DAJTYF010000000
VERSION     DAJTYF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.378"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..263)
                     /locus_tag="OO590_004393"
     CDS             complement(<1..263)
                     /locus_tag="OO590_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6639472.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQ"
     gene            complement(263..>415)
                     /locus_tag="OO590_004394"
     CDS             complement(263..>415)
                     /locus_tag="OO590_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6639473.1"
                     /translation="KSIAFANMDETEFQQVYKSVLNVLWNWILFRKFSSPEEVENVAA
                     QLLEFA"
BASE COUNT           94 a          112 c           95 g          114 t
ORIGIN      
        1 tcttgtgtgc gaaaaacacc ttctgcatgc atcaggcgta attcttcttt ggtgtaatcg
       61 ctggttttta cccgcccgtc gattaaatcg tggcatgagc tgcaggcaat cgctgcctgc
      121 atatcgtgtg gttttgtcgc tgttccgcac gtccccgcca gcctgtaatg cgccagcaca
      181 gaagtttcgg gattgtgatt gcagtagcca ggaattctga cggtgcacat ctgcccccgc
      241 gccgctttac gtaaatccac cattacgcaa actccagtaa ctgtgcggcc acattttcga
      301 cttcctccgg agaggaaaat ttacggaaca ggatccagtt ccacagcaca ttcagtacag
      361 atttataaac ctgctgaaac tcggtttcgt ccatgttcgc aaatgcgata gattt
//
LOCUS       DAJTYF010000372          411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTYF010000372 DAJTYF010000000
VERSION     DAJTYF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.379"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..>411)
                     /locus_tag="OO590_004395"
     CDS             complement(<1..>411)
                     /locus_tag="OO590_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR6639474.1"
                     /translation="VFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSL
                     AMGMMFDSRNIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSID
                     APLKVLLGDADSKYIPASLCRTNASGTPVNGYFLT"
BASE COUNT          117 a          108 c           87 g           99 t
ORIGIN      
        1 gtcagaaaat agccattaac gggcgtacca gaagcgttgg tacgacataa actggctgga
       61 atatatttgc tgtcagcatc acctaatagc actttaagcg gggcatcaat cgagaatacc
      121 agcgcagcta cttgtcccag ggtattcgca atggcgtaaa tcaccattaa agtattaccc
      181 atgttgtaat actcgcccag cttctgaaag gcgtaatatt gaccgttcgt cattaagtca
      241 tccgggatat tacgcgagtc aaacatcatc cccatcgcca gtgagcccag aatggcacaa
      301 accgcaacca tcaccgccag gcatagcatc ccttttggaa attcttttcc tgggttgcgc
      361 gtttgattaa cgtaaggaga aatcttctct gctccgccaa ccgcgaaaac c
//
LOCUS       DAJTYF010000373          408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTYF010000373 DAJTYF010000000
VERSION     DAJTYF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.380"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(7..225)
                     /locus_tag="OO590_004396"
     CDS             complement(7..225)
                     /locus_tag="OO590_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639475.1"
                     /translation="MKAVARANNHPYKSVFADFITGHPSCTVCFWETFHKMYPDSPYE
                     YVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT           90 a           99 c          108 g          111 t
ORIGIN      
        1 tgaaaatcac caccacttcg ggtcatccgc cttcatctcc gcttctgttt cgtataaatc
       61 aaaacgacgg caggtatggc agaacgtgac gtattcgtac ggtgaatccg ggtacatttt
      121 gtggaaggtt tcccagaaac agaccgtgca ggagggatga ccggtgataa agtctgcaaa
      181 tacggactta taagggtgat tattggctct ggcgacggct ttcagaaccc tctttcacca
      241 ttctggtgtg gactttctgg tgctccaggt tgtgtgacat gggaactcat tctggatggt
      301 tactctgaaa gctcatattc tgccacaccc cgatttgcag ccgccaggct gccgtggttc
      361 ttacgccaga agggatttgt aactggtctg gtcggctccg gcgtatca
//
LOCUS       DAJTYF010000374          403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTYF010000374 DAJTYF010000000
VERSION     DAJTYF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.381"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>403
                     /locus_tag="OO590_004397"
     CDS             <1..>403
                     /locus_tag="OO590_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639476.1"
                     /translation="TAYVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALA
                     GKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGL
                     QETVNKADNAVQKTGDTLSGGLTFENDSILAW"
BASE COUNT           93 a          108 c          114 g           88 t
ORIGIN      
        1 acggcttatg ttatggccgc gattgctgcc ctcgtggact cgtcgcctga tgcactgaac
       61 acgctgaatg aactggcggc ggcgctgggc aacgacccga attttgcgac caccatgact
      121 aacgcgcttg cgggtaagca accgaaagat gccactctga cggcgctggc ggggcttgct
      181 actgcggcag acaggtttcc gtattttacg gggaatgatg tcgccagcct ggcaaccctg
      241 acaaaagtcg ggcgggatat tcttgcgaaa tcgaccgttg ccgccgttat cgaatacctc
      301 ggtttacagg aaacggtaaa caaggctgat aacgctgtcc aaaagacagg cgataccttg
      361 tccggtggac ttacttttga aaacgactca atccttgcct gga
//
LOCUS       DAJTYF010000375          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTYF010000375 DAJTYF010000000
VERSION     DAJTYF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.382"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..241
                     /locus_tag="OO590_004398"
     CDS             <1..241
                     /locus_tag="OO590_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6639477.1"
                     /translation="QPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSI
                     GPVERFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT          110 a           90 c           83 g          112 t
ORIGIN      
        1 gcaacctgaa actggattca ccggtcgcga tatgatgcgc accgatgtgg tcaatgccgt
       61 gctggaaaaa gagtgcgaag cgatagtcct gactgacagc tcgaaatttg gtgctgtaca
      121 ttcatactcc atcggtcccg ttgagcgatt caatcgcgtg attaccgatt cgaaaatacg
      181 cgccagcgat ctgatgcatc ttgagcacag taaactcacc gttcacgtcg ttgacattta
      241 accatttccc ttctctacgg atgatttgca gtttggcaaa tcatccgctc taagatgatt
      301 cctggttgat aattaagact atttacctgt tattaacact ctcaagatat aaaattatta
      361 tcagcgatat aacaggaagt aattatcacg tgcgt
//
LOCUS       DAJTYF010000376          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTYF010000376 DAJTYF010000000
VERSION     DAJTYF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.383"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
BASE COUNT          128 a           48 c           77 g          142 t
ORIGIN      
        1 tgttgttaat aaaaagctta tagatttata gttgataatt aaaacgcatc cgatatttca
       61 gctttttcta tgcaagatac aattttataa ggtgtccatt tttatatgga ggcggtagcg
      121 tgtctgttac tagtggtttt atttgcatag tgaatatatt gggattgtta aatacataac
      181 tatgtatccg ctctggtcgt tgtaaatatt gctaattgct taatattatt agtgattatg
      241 gctggaaaga taaagtgtgc tgtaaagcgg tgatgggtat tacattcatg attaaaaaaa
      301 cagaacctgt caataaataa ttcctttatt aaaacggact atttacatta tgacggaata
      361 ttatctcaac gaggcggttt ggaaatacag agcca
//
LOCUS       DAJTYF010000377          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTYF010000377 DAJTYF010000000
VERSION     DAJTYF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.384"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            84..365
                     /locus_tag="OO590_004399"
     CDS             84..365
                     /locus_tag="OO590_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639478.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVIAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHGKKPERLRR"
BASE COUNT          113 a           85 c          122 g           74 t
ORIGIN      
        1 gcatgaggat tttgcttttg atataagcga cagccaccgc cagaaactgg aagaatttag
       61 cggggtgtaa tgaggtcagc agcatgacag aagccgaaat aacgggatta atccgccgcg
      121 ttgccggaat cagccagcag gctgacgaac aggccacgca gccggacagc gtgatagccg
      181 aaaactatgc gcgtgttgtt gctgaggtga tgcgccgtga tggtatagaa cttaatggcg
      241 tggatatgcg caacatacga accagagttc ttgagttgct ggcctaccgt cgccgcgtgg
      301 agatgtatcg ggagaaggag aaaatcacgt accacgggaa gaagccggag cggttacggc
      361 ggtaactcac tgagattaaa gcaaagcgca aaat
//
LOCUS       DAJTYF010000378          389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTYF010000378 DAJTYF010000000
VERSION     DAJTYF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.385"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..294
                     /locus_tag="OO590_004400"
     CDS             <1..294
                     /locus_tag="OO590_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6639479.1"
                     /translation="VNKALRVMGYDTKKDICGHGFRAMACSALMESGLWAKDAVERQM
                     SHQERNTVRMAYIHKAEHLEARKAMMQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT          115 a           86 c           99 g           89 t
ORIGIN      
        1 gtcaataagg cactgcgggt gatgggttac gacacgaaaa aggatatctg cggtcacggc
       61 ttccgggcaa tggcatgcag tgcgctgatg gaatcgggtt tatgggcaaa ggacgcagta
      121 gaacgccaga tgagtcatca ggagcgcaat accgtgcgca tggcttatat tcataaggca
      181 gagcacctgg aagcccgcaa agcgatgatg cagtggtggt cggattatct ggaagcatgc
      241 cgagaatctt atgcaccgcc ttatacaatt ggtaaaaata agtttatccc atagtatact
      301 aacgaatccc acactctggc aaattaccac cacctctaca ggaggtggtt ctatatagac
      361 ttttatcaac tttatgacga aaaacacct
//
LOCUS       DAJTYF010000379          388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTYF010000379 DAJTYF010000000
VERSION     DAJTYF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.386"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>388
                     /locus_tag="OO590_004401"
     CDS             <1..>388
                     /locus_tag="OO590_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR6639480.1"
                     /translation="SAASSASSASASKDEATKQASAAKGSATTASSKATESAGSATAA
                     AQSKSAAESAAMRAETAAKRAEDIADVVSLEDASTTKKGIVQLSSATNSASESLAATA
                     KAVKVVMDETNKKAPLNSPALTGTPTT"
BASE COUNT          110 a          102 c          123 g           53 t
ORIGIN      
        1 gttcggcagc gtcatcggca tcatctgcgt ctgcttcaaa agatgaggcg actaaacagg
       61 cgtcagcagc gaagggcagt gccacgacgg catcctctaa ggcgacagag tctgcaggca
      121 gtgcgacggc ggcagcgcag agcaaaagcg cggcagaatc cgcggcaatg cgcgctgaga
      181 cagcagcaaa acgggcagag gatattgctg atgtcgtatc tctggaagat gcgagcacga
      241 cgaaaaaggg gatagtgcag ctcagcagtg cgaccaacag tgcatccgag tcactggccg
      301 cgacagcgaa agcggttaag gtggtaatgg atgaaacaaa caagaaagcg cccctgaaca
      361 gtccggctct gaccggaacg ccaaccac
//
LOCUS       DAJTYF010000380          379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTYF010000380 DAJTYF010000000
VERSION     DAJTYF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.387"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..379
                     /locus_tag="OO590_004402"
                     /pseudo
     CDS             1..379
                     /locus_tag="OO590_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          118 a           80 c          121 g           60 t
ORIGIN      
        1 ggcagcacag cgataaatgg atgaccgaat ccaggcagga aaagctgata tggctgcgtg
       61 cgcagatgaa gctgacgagc ctgtgctggg cgctgaaaga actggcaaag gatatctgga
      121 gcagaccatg gagcgaggaa agacgcaacg actggcagag atggctgcgg ccaacagtga
      181 cgtccccatg atgaagaatg tggctaaaac gatagggaaa aggttgtacg gtatcctgaa
      241 cgccatgcgc catggcgtat caaacggaaa tgcggaagca ctgaacagca agatcaggct
      301 gctgagaata aaagccaagg gctaccgcaa ccgggagcgc tttaagctgg gagtgatgtt
      361 ccactatggg aaactgaat
//
LOCUS       DAJTYF010000381          356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTYF010000381 DAJTYF010000000
VERSION     DAJTYF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.388"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>356
                     /locus_tag="OO590_004403"
     CDS             <1..>356
                     /locus_tag="OO590_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6639481.1"
                     /translation="KTFVYYLYPVTHNRSLIFLLGLERFSLLSKSLAMDSENLMFSLF
                     KNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALV
                     AVISGASCLYLVRRVI"
BASE COUNT           84 a           72 c           83 g          117 t
ORIGIN      
        1 gtaaaacctt cgtctattac ctctatccgg taactcacaa tcgtagtctg atatttttgc
       61 ttggtctgga gcgtttttct ttactgtcaa aatcgctggc gatggacagc gagaacctga
      121 tgttctctct atttaagaac ggtaaaccgg tgaccggaga tgaatataat gctaaaaacg
      181 ccatcttcac cgtttcggaa gcgatggagc acttcgccta tttgccgacc ggattgtacg
      241 tatttgcgta taaaaaagat gtttatttgc gggtttgtac attgattatt ttctttgccg
      301 cattggtggc agtgatatcg ggtgccagtt gcctctatct ggtacgcaga gtgatt
//
LOCUS       DAJTYF010000382          354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTYF010000382 DAJTYF010000000
VERSION     DAJTYF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.389"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..212)
                     /locus_tag="OO590_004404"
     CDS             complement(<1..212)
                     /locus_tag="OO590_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR6639482.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLT"
BASE COUNT          119 a           89 c           74 g           72 t
ORIGIN      
        1 aaggtgagat atttaatgga aaccacaaag attagcagcc agaagatcag cgataaaaag
       61 ccaaacacgg catcgcgttc aacgccaaaa ccaaactggc cggacaaaca ttcacgaagt
      121 gtatataacg ggctggtacc aatatcgccg tagacaactc caatcgccgc gagggtaatc
      181 gcgggcaatg attgcttatt atcagtgctc atagactagt ctttcgttga aatatgaaat
      241 gtgtgcttag tcccttggcc cacaaaaaag cgcacagtat gcacgattaa cggcaaaatc
      301 gtactcctaa atgcggccac attaacctgg ctcaaagaaa aagccccccc cctt
//
LOCUS       DAJTYF010000383          352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTYF010000383 DAJTYF010000000
VERSION     DAJTYF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.390"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
BASE COUNT          114 a           86 c           75 g           77 t
ORIGIN      
        1 gcatgattcc agtccatgca aaatctgatt tctagctcac acataccgag cgctgtacaa
       61 aaaaccgcca gccaaggcag ttaagagtgt gtccccgggg tttgcttaga atattttgta
      121 agttgtccga agagatattt acaacaccag aatgatgaat catcagtccc ctgccagagg
      181 ttcactacgt aacctgaccg acaatgcacc attctggtgc tgaaaaaaga gcactggaac
      241 tgcaacaaaa caacatcaca gaacaggaga tggtaaggag tcagacattc ccacacaata
      301 ttgtgtcagt gcaaataaca accttcgtct caaatctcgc tggagcgggc ag
//
LOCUS       DAJTYF010000384          350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTYF010000384 DAJTYF010000000
VERSION     DAJTYF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.391"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            54..236
                     /locus_tag="OO590_004405"
     CDS             54..236
                     /locus_tag="OO590_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6639483.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT           87 a           68 c          103 g           92 t
ORIGIN      
        1 ttcgccatct tcaactaagg tgaacaagtt tcactcgaaa aaaggaggcg agtatgagct
       61 ttatggttag tgaggaagtt acggttaaag agggcggccc gcgaatgatt gtcactggat
      121 actccagcgg tatggttgag tgtcgttggt atgacggtta cggggtcaag cgggaagctt
      181 ttcatgaaac cgatcttgtt ccgggggagg ggagtcgttc tgcggaagaa gtttgaaagg
      241 gtatcattaa caccaggccg gatcacatcc ggcctggtgt cccaatgcct gcaatgaatg
      301 atagtcgcgt cttatcatgc caccattata aaattatcgc tgttttgcgg
//
LOCUS       DAJTYF010000385          346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTYF010000385 DAJTYF010000000
VERSION     DAJTYF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.392"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            24..100
                     /locus_tag="OO590_004406"
     tRNA            24..100
                     /locus_tag="OO590_004406"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="OO590_004407"
     tRNA            143..218
                     /locus_tag="OO590_004407"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           98 a           72 c           87 g           89 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg agagttgttc gtgagt
//
LOCUS       DAJTYF010000386          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTYF010000386 DAJTYF010000000
VERSION     DAJTYF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.393"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="OO590_004408"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="OO590_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR6639484.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           91 a           81 c          100 g           73 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatg
//
LOCUS       DAJTYF010000387          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTYF010000387 DAJTYF010000000
VERSION     DAJTYF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.394"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>340
                     /locus_tag="OO590_004409"
     CDS             <1..>340
                     /locus_tag="OO590_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6639485.1"
                     /translation="EEIFTREEAASYLKVDKGTITQWIRSGRLQAVKINPDKPKSPYR
                     ICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLKHR
                     TKGKRKNFTIV"
BASE COUNT          105 a           75 c           91 g           69 t
ORIGIN      
        1 agaagaaatc ttcactcgtg aagaggcagc gtcgtatctg aaggtagaca aaggcactat
       61 cacgcagtgg atacgaagtg gacgacttca ggccgtaaag ataaatccag ataaacctaa
      121 aagcccatat cgcatttgca agtcagactg cattgcggcg cttaagtctg tgagacacaa
      181 tagcgcggtg aatgcggttg atgtgcagga ggttaaagca tgtcaatcaa actacgcggt
      241 ggcacgtggc actgcgattt cgtcgcgcca gatggatcaa gagttagacg ctctcttgaa
      301 acatcggaca aaaggcaagc gcaagaactt cacgatcgtc
//
LOCUS       DAJTYF010000388          338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTYF010000388 DAJTYF010000000
VERSION     DAJTYF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.395"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>338
                     /gene="fimC"
                     /locus_tag="OO590_004410"
     CDS             <1..>338
                     /gene="fimC"
                     /locus_tag="OO590_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6639486.1"
                     /translation="GVKDGRFIVTPPLFAMKGKKENTLRILDATNNQLPQDRESLFWM
                     NVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPPDQAAEKLRFRRSANSLTLI
                     NPTPYYLTVT"
BASE COUNT          104 a           76 c           73 g           85 t
ORIGIN      
        1 tggtgtaaag gatggtcgtt ttatcgtgac gcctcctctg tttgcgatga agggaaaaaa
       61 agagaatacc ttacgtattc ttgatgcaac aaataaccaa ttgccacagg accgggaaag
      121 tttattctgg atgaacgtta aagcgattcc gtcaatggat aaatcaaaat tgactgagaa
      181 tacgctacag ctcgcaatta tcagccgcat taaactgtac tatcgcccgg ctaaattagc
      241 gttgccaccc gatcaggccg cagaaaaatt aagatttcgt cgtagcgcga attctctgac
      301 gctgattaac ccgacaccct attacctgac ggtaacag
//
LOCUS       DAJTYF010000389          333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTYF010000389 DAJTYF010000000
VERSION     DAJTYF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.396"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
BASE COUNT           87 a           79 c           97 g           70 t
ORIGIN      
        1 ccctgaagtc gcaccccact gacctactca agcccccacg taaagggaga ccagcaggca
       61 acaggacgca aacagaatga cttgaggtaa ttggggttag tggtctgcga tgaccataga
      121 gatgaaggtt cgacagcatg tccgagcgtg tgtggtgata gacttaagtc acgagaccaa
      181 agaacacaca ggctgaccag aggctgtctt gccttaggct ggtggctagc ttgctagcgt
      241 ggaccacggt ttctcgcttg ccgtctctca ggaggtcaga ggcgagatat ggtccctata
      301 gtgatagttt agtcaaacag gaatgaactt cgg
//
LOCUS       DAJTYF010000390          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTYF010000390 DAJTYF010000000
VERSION     DAJTYF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.397"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..115)
                     /locus_tag="OO590_004411"
     CDS             complement(<1..115)
                     /locus_tag="OO590_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR6639487.1"
                     /translation="MKSMDKMSTGIAYGTSAGSAGYWLLQWLDQVSPSQWAA"
BASE COUNT          105 a           83 c           78 g           66 t
ORIGIN      
        1 tcgcagccca ctgtgacgga ctgacctgat caagccactg taaaagccag tagccggcac
       61 tgccggcgga ggtgccgtag gcaatgcccg ttgacatttt gtccatagat ttcataacct
      121 cacctccgca aataacggat ggcgtagctt tacactgaga gacgaaaagg atttgaaaaa
      181 aagcccgcaa aagcgggcgc aacggaatct acagtaagga aagcactcta tccaacaaac
      241 cacccacagt taatcggaat aaaagcagag tgcttatgaa tgatcgcctg cccgaaggtt
      301 ggtatttctg cacagcaatt ttgcaaaaaa gt
//
LOCUS       DAJTYF010000391          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTYF010000391 DAJTYF010000000
VERSION     DAJTYF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.398"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
BASE COUNT          108 a           59 c           60 g          100 t
ORIGIN      
        1 gtttaagctc cattttcaaa ataatatatc agggtttgtt actaataatt tctggaaaca
       61 gaaaaagtag agcagtaatt ctatttcaga gaggagtaac tccatctatc ctcgatacat
      121 actgaacgtt atcgtgatat atttatcatg cgttcttcac cattattcga gtgcagggac
      181 gcaatgacct taaatacctt ttagtagaat atattgctga cccacaaata tcaggatggt
      241 attgacaagt catagatgga gaaatatatg actgaattac cattctctgg aataacagca
      301 gactccttcc catagacggc ttagggg
//
LOCUS       DAJTYF010000392          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTYF010000392 DAJTYF010000000
VERSION     DAJTYF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.399"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>323
                     /locus_tag="OO590_004412"
     CDS             <1..>323
                     /locus_tag="OO590_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6639488.1"
                     /translation="VLGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAF
                     LKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLLGGLSCLTIPCFAWLA
                     DRFGRR"
BASE COUNT           72 a           73 c           84 g           94 t
ORIGIN      
        1 gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt tgaacaacag
       61 caacataatc aagcggccgc gaaaaaacgc atcccggtta tcgaagctct gttacgacat
      121 cccggtgctt tcctgaagat tattgcacta cgactgtgcg agttgctgac gatgtacatt
      181 gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggttaccgcg cgaacttttc
      241 cttaatattg gtttgctgtt aggtggatta agctgcctga caattccctg ttttgcctgg
      301 cttgccgatc gttttggtcg gcg
//
LOCUS       DAJTYF010000393          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTYF010000393 DAJTYF010000000
VERSION     DAJTYF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.400"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(<1..>322)
                     /locus_tag="OO590_004413"
     CDS             complement(<1..>322)
                     /locus_tag="OO590_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6639489.1"
                     /translation="MDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTK
                     REEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVRKTAM
                     QITWR"
BASE COUNT           73 a          101 c           61 g           87 t
ORIGIN      
        1 ctctccaggt gatttgcatc gccgtttttc tcactgtctc cgttatgatg tacaccactt
       61 cttccagtaa ccgccgtttc gccggactca aaaaaccgta gtacctcacc atacggaacc
      121 ccttatccgc cacatgccag gagaaccttt ccatgaactc atctccactc atcaacaggt
      181 attcttcccg ttttgttcgg tgactgttgt aacgcagacc gatttcatcc tgaccggcat
      241 aatgctccag acgactcatc ggcaccggtg gctttttcag gtaagagcca aagtacaccg
      301 ccacatgggt ggcattatcc at
//
LOCUS       DAJTYF010000394          317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTYF010000394 DAJTYF010000000
VERSION     DAJTYF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.401"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..229
                     /locus_tag="OO590_004414"
                     /pseudo
     CDS             1..229
                     /locus_tag="OO590_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           78 a           75 c           90 g           74 t
ORIGIN      
        1 cattgctgat gtgtcagtcg gccgcctgtt tgtgctggtc aataaatttg atgaaaaaga
       61 ccgtaatggc gatggggctg atgcagtctg tcagaaggta ccggcaatgc tgaacagcga
      121 tgtgctgccc gcctcccgcg tttatcccgg atcctcacgc caggcatacc tggctaaccg
      181 tgcgcttcat gagttacgga aaaacggaac ccttcctgtt gatgaagcct aggtcgatga
      241 ttttgtcagg gaagccttcg gtcgcatgaa aaaagattac gtctgtaaag acagcgaact
      301 ggcaactgaa ggggcga
//
LOCUS       DAJTYF010000395          309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTYF010000395 DAJTYF010000000
VERSION     DAJTYF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.402"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            1..291
                     /locus_tag="OO590_004415"
                     /pseudo
     CDS             1..291
                     /locus_tag="OO590_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    249..>309
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT           70 a           76 c           96 g           67 t
ORIGIN      
        1 cgtcaacggg cagtccgtat ggttctggaa agtcagggcg aatatgactc acaatgggcg
       61 acaatttgtt ccattgctcc aaagattggc tgtacgccgg agactctgcg tgtctgggtt
      121 cgccagcatg agcgggatac cgggggcggt gatggagggc tcaccaccgc tgaacgtcag
      181 cgtctgaaag agctggagcg tgaaaatcgt gaactgcgcc gcagtaacga tatccttcgc
      241 caggcttccg cttattttgc gaaggcggag ttcgaccgcc tctggaaaaa atgatgccac
      301 tgctggata
//
LOCUS       DAJTYF010000396          307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTYF010000396 DAJTYF010000000
VERSION     DAJTYF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.403"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
BASE COUNT           92 a           62 c           46 g          107 t
ORIGIN      
        1 gatgctctga ctcgcaattc atccagcagt ttatcatttc ccataatgtc acttaccctc
       61 atcggtaaaa aaattgcctt tcgaccatta cgatacatta ttgattttgg ggtttcaggg
      121 aagtaatcca tttcgactat aactgacagg tcatcacgac gtatgactgc gtatttgcta
      181 ctaaatagtt tctttatttt ctccacgatg cctccaggtt tataagtaca aacggttata
      241 tccacataga gacaaaaata ttaatctgaa aaatatttat ttcacgccgt atatttgatt
      301 gtttaat
//
LOCUS       DAJTYF010000397          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTYF010000397 DAJTYF010000000
VERSION     DAJTYF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.404"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>304
                     /locus_tag="OO590_004416"
     CDS             <1..>304
                     /locus_tag="OO590_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6639490.1"
                     /translation="VTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNELP
                     ASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRSPESNGIAESFVKTIKRDYI"
BASE COUNT           71 a           73 c          104 g           56 t
ORIGIN      
        1 gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg gtcactacgg
       61 gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa cgccgcttcg
      121 gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca tgctaccggg
      181 ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagagc acggcggtgc
      241 ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag cgtgactaca
      301 tcag
//
LOCUS       DAJTYF010000398          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTYF010000398 DAJTYF010000000
VERSION     DAJTYF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.405"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..>303
                     /locus_tag="OO590_004417"
     CDS             <1..>303
                     /locus_tag="OO590_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6639491.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRR"
BASE COUNT           91 a           52 c           94 g           66 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggg
//
LOCUS       DAJTYF010000399          297 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTYF010000399 DAJTYF010000000
VERSION     DAJTYF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.406"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(1..297)
                     /locus_tag="OO590_004418"
                     /pseudo
     CDS             complement(1..297)
                     /locus_tag="OO590_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT           91 a           70 c           46 g           90 t
ORIGIN      
        1 tcgccacttc accgggctca ggaccccgta ggatcttcac cgaaaaaaat gcatcaccag
       61 ttgctttatt atttgtttta ggtagacctg ttactaattt atacctactc tgaccaagtc
      121 tggataagaa aggcacacct gcacatttct acttggaaat gttttctttt accattttgc
      181 tcaaaaattt caacatccaa tattcctcgt acagaactac tcaagtcctg aatagaaaaa
      241 gttccagctg gtacagtaga gtcatatatt actcgacctt gttgagatac aacaaca
//
LOCUS       DAJTYF010000400          295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTYF010000400 DAJTYF010000000
VERSION     DAJTYF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.denovo.407"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            complement(26..280)
                     /locus_tag="OO590_004419"
     CDS             complement(26..280)
                     /locus_tag="OO590_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6639492.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
BASE COUNT           66 a           71 c           85 g           73 t
ORIGIN      
        1 tttgcttact gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc
       61 aggtcatggc ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac
      121 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
      181 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
      241 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaa
//
LOCUS       DAJTYF010000401         7263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.guided.165, whole genome shotgun sequence.
ACCESSION   DAJTYF010000401 DAJTYF010000000
VERSION     DAJTYF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.guided.165"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..344
                     /locus_tag="OO590_004420"
     CDS             <1..344
                     /locus_tag="OO590_004420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6639493.1"
                     /translation="IAAWAQIDESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDML
                     FVDPEYTRRGVASALLKPFIKSESELTVDASITAKPFFERYVFQTVKQQRVECRGAWF
                     INFYMRYKPQH"
     gene            590..1634
                     /locus_tag="OO590_004421"
                     /pseudo
     CDS             590..1634
                     /locus_tag="OO590_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1696..2310
                     /locus_tag="OO590_004422"
                     /pseudo
     CDS             1696..2310
                     /locus_tag="OO590_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2367..3824)
                     /gene="pepD"
                     /locus_tag="OO590_004423"
     CDS             complement(2367..3824)
                     /gene="pepD"
                     /locus_tag="OO590_004423"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6639494.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4086..4544
                     /gene="gpt"
                     /locus_tag="OO590_004424"
     CDS             4086..4544
                     /gene="gpt"
                     /locus_tag="OO590_004424"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6639495.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4636..5880
                     /gene="frsA"
                     /locus_tag="OO590_004425"
     CDS             4636..5880
                     /gene="frsA"
                     /locus_tag="OO590_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6639496.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5938..6339
                     /gene="crl"
                     /locus_tag="OO590_004426"
     CDS             5938..6339
                     /gene="crl"
                     /locus_tag="OO590_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6639497.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6378..>7263)
                     /gene="phoE"
                     /locus_tag="OO590_004427"
     CDS             complement(6378..>7263)
                     /gene="phoE"
                     /locus_tag="OO590_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6639498.1"
                     /translation="RFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAG
                     LKYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDF
                     FGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQ
                     NLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQ
                     FDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDN
                     KLNINNDDTVAVGMTYQF"
BASE COUNT         1762 a         1784 c         1869 g         1848 t
ORIGIN      
        1 aaattgccgc ctgggcgcag attgacgaat ctcgctggaa ggagaaactc gcgaaatcac
       61 aagtgcgggt tgcagtcatt aatgcaaaac tggtcggctt tattacctgc gttgaacact
      121 atatcgatat gttatttgtt gaccctgaat acacccgccg tggggttgcc agcgctttgt
      181 taaaaccttt tattaagtct gaatccgaac ttacggtgga cgcaagcata accgcaaaac
      241 ccttttttga acgttatgtt tttcagacag ttaagcagca gcgcgttgaa tgccggggag
      301 cgtggtttat taatttttat atgcgttata aaccgcaaca ttaaatccag cttgcaatga
      361 aaataacgcc cgcctagtat gtgcgcgttt tcttatttca caaactgcaa ggaggtaaat
      421 catgacaaat cctttatcaa tgactctttg cagacctttc caggattaat tctttttttc
      481 ttgccctgga ttcgtctgcc atttcctgat tttttaattg ttactctcaa taattcaagt
      541 cgcagcacat gcaacttgaa gtatgacgag tctatttacg gaatggtata tgggcaatta
      601 tattcgtccc ttatccgatg cggtatttac catcgcatct gatgacctgt ggatcgagag
      661 tttagcgatc caacaattac acaccacggc aaatttaccc aacatgcagc gcgtagtggg
      721 gatgccagat ttacaccccg gacgcggcta cccgattggc gcagcgttct tctccgcagg
      781 ccgtttttac ccagcactgg tcggcaatga tatcggctgt ggcatggcgc tatggcaaac
      841 agatattctc gctcgcaaat acaacgccga taagtttgaa aagcgattat cagctttgga
      901 tgacgttgct gaagaaagct ggctggagga aaacctgccg tcagcatttg cacagcatcc
      961 gtggcgcagc tcgcttggct ctatcggtgg tggcaatcat ttcgctgaac tgcaacaagt
     1021 tgatcaaatc atcaacgctg aactgtttgc actggcaggt ctggatgcgc agcatctgca
     1081 actgctggtt catagcggct cgagggagct agggcagtct attttacagc ggcatattgc
     1141 ctcgttttcg catcatggtt tgcctgaagg cagtgacgac gcgctggcgt ttgcgcgttt
     1201 taatcgccac ctgatcgctt tgcgcataat gcaacaggtt aaggccaccg gaagtccggt
     1261 tctggatgtg gcgcataact ttgttagcgc gtgtcgaatc ggtgatcaac aaggggtctt
     1321 acatcgtaaa ggtgccacgc ctgatgactg tggtctggtg gttattcccg gttcacgcgg
     1381 tgattactcc aggctggttc aggcccgtca ggagtgagga aacattgcat tcgctggcgc
     1441 atggggctgg gcgtaaatgg gggcgcaccg agtgtaaagg gcgtctggca gcgaaataca
     1501 cagcgacgca actctcgcgt actgaacttg gcagtcgggt aatttgtcgc gataaacaac
     1561 tcatcttcga agaagcgcca caagcctata aatcggctga aagtgtggtg caatgtctgg
     1621 gcaggccgga ttaattattc ctgtcgcgcg gctgcgtccg gtgctaacgc tcaaaaacag
     1681 tggagggaaa aaaggatgat cttgctataa ctctcctcag cgcaggggcc ggaagaatgt
     1741 tgtctcgcag tgaaaaaggc gctggacagg ctaattaaag aagctgcccg acaagacgtc
     1801 gcggtaacgg tgctggaaac agaaacaggc cgctactctg acacgctgcg ttcggcgctg
     1861 atttctctgg atggcgacaa cgcatgggcg ttaagcgaaa gctggtgtgg cactattcag
     1921 tggatttgcc cgagcccgta tcgcccacat cacgggcgta aaaactggtt tctgggcatt
     1981 gggcgtttta ccgctgatga gcaggagcaa tccgatgcga tccggtatga gacgctgcgt
     2041 tcgtcggggc cgggcggtca acatgtcaat aaaaccgact cggcggtacg cgccacgcat
     2101 ttggcatccg gtattagcgt gaaggttcag tcagagcgca gccagcatgc taacaaacgg
     2161 ctggcgcgat tgctgattgc ctggaagctg gatcagcagc aataggaaaa tagcgcggtg
     2221 ctgaaatcac agcggcgaat gttccatcac cagattgaac gtggcaaccc gcgacgaacg
     2281 tttacaggga tggcttttat cgaaggataa tgaaggatga aactgccgga aggcgattaa
     2341 acgccatccg gcagcaaatc aaataattac ttcgccggaa tttctttcag cagttcagtc
     2401 agcagtgtcc agtaatgacc tacgctttcg atgtgaactt gctcatccgg agagtgtgga
     2461 ccggtgatag ttggcccgat agaaaccatg tccatttccg gatacggttt tttgaacaga
     2521 ccacattcca ggcccgcgtg gataatctgg atgttcggcg tcttgttgaa caggcgctga
     2581 taggtttcac gtaccagatg catcaccgga gaattagcgt ccggctgcca gccaggatat
     2641 gcgcctttcg cttcggtttt cgcgccagcc agtttaccca gcgaatccag catgctcacc
     2701 acgtagtctt taccgctgtc gatcagtgaa cggatcaggc agtgaatttc tacgttattg
     2761 tcagtcatgg tcaccacacc gacgttcagg gaggtttcga ccacaccttt tgccacgtcg
     2821 gaattgcgga tcacaccgtt cggggtggcg ttcagcagac gaataaaggt atcgcgagat
     2881 ttcgcaatca gggcagcttt atcgttcgct acagagtcca gcaacaaggc cagatttttc
     2941 tctttctctg ccagctcgtt tttcaggatc tcctgatagg tattcaccag agatttcagt
     3001 gcgtcgactt tatcagctgt gacagcaatg gtcgcaaagg cttcacgcgg gatggcgtta
     3061 cgcagtgtgc cgccgttgaa atcgataagg cgcaggtcca gttcttctgc atgacccgcc
     3121 aggaagcgca ccagcagttt gttggcatta cccaggccaa cgtggatttc accgccggag
     3181 tgaccgcctt tcagaccttt taaggttaac ttgaaggttt caaaaccagc tggaaccgct
     3241 tcacgatcta aatgcaggtt ggaggtgaag tcgatacccc ccgcacaacc catgtagatt
     3301 tcaccttctt cttcggagtc ggtgttaatc agaatatcag cctgcaacca gttgctctgt
     3361 aagccgaacg caccgtccat accggcttct tcggttatgg tcagcagcac ttccagcggg
     3421 ccgtgaacca cgttttcgtc agccagaacc gccagtgcag aggccatacc aatgccgtta
     3481 tccgcaccca gcgtggtgcc gcgcgcttta acccattcgc catcaatata aggctggata
     3541 ggatctttcg tgaagtcatg cacggtgtcg ttatttttct gcggcaccat atcgaggtgg
     3601 gcctgtaaga cgaccggttt acgattttcc atacctgcgg tagcaggttt acgaatcagg
     3661 atattaccta cctgatcgcg ttcgacatgg aaacctttct cttttgccca accaacaatg
     3721 tattcagcga gttgctcttc atgataggac gggtgaggaa tagaacagat tttggcaaaa
     3781 atatcccaca gcggctgtgg agataattga gacagttcag acacgttaag tctccttgtc
     3841 gatcacccgc aaaacagtat tgcaggtcac agggttagca gaaaatgttg tcaacacaag
     3901 acaggcttgc gagatatgta ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     3961 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat atctataatc
     4021 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     4081 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     4141 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     4201 gtctggtacc gggggcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
     4261 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
     4321 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgctgttg
     4381 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
     4441 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatc gaacagccgt
     4501 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     4561 cattgccggg cgttgttctt tttaacttca ggcaggttac aatagtttcc agtaagtatt
     4621 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     4681 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
     4741 cagtcggctc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
     4801 tggatctggc gcggtattga cccacgtgaa atcctcgacg tccaggcacg tattgtgatg
     4861 agcgatgctg aacgtaccga cgatgattta tacgataccg tgattggcta ccgtggtggc
     4921 aactggattt atgagtgggc cacccaggcg atggtgtggc aacaaaaagc ctgtacggaa
     4981 gaagatccgc aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta caacattgcc
     5041 gcctatcctc atctgaaagg agatgacctg gccgagcaag cgcaggcttt atcaaaccgc
     5101 gcctatgaag aggccgccca gcgtctgccg ggcacgatgc ggcagatgga gtttaccgta
     5161 cccggcggtg cgcccatcac cggctttttg catatgccgc aaggcgatgg cccgttcccg
     5221 acagtattaa tgtgtggtgg tctggatgcg atgcagacgg actattacag cctgtatgaa
     5281 cgttattttg cgccgcgcgg cattgcgatg ctgactattg atatgccgtc ggtgggcttt
     5341 tcttcaaaat ggaagctcac ccaggactcc agcctgttgc atcagcacgt cttaaaggcg
     5401 ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc
     5461 gctaacgttg ccgtgcgtct ggcatacctt gaatcgccgc gtctgaaagc ggttgcctgt
     5521 cttggtccgg tagttcatac cctgttgagt gattttaagt gccagcaaca ggtgccggaa
     5581 atgtatcttg acgttctggc gagtcgtttg gggatgcatg atgcttccga cgaagcgtta
     5641 cgcgtggagc tgaatcgcta ttcattaaaa gtgcaaggat tgctggggcg tcgctgccca
     5701 acgccaatgt tatcaggcta ctggaagaac gatccgttca gcccggaaga ggactcacgc
     5761 ttaatcacct catcatctgc tgacggtaaa ttattagaga tcccatttaa cccggtgtat
     5821 cggaattttg acaaaggtct tcaggaaatc accggctgga tcgaaaaacg cttgtgttaa
     5881 aaatttgcta aattttgcca atttggtaaa acagttgcat cacaacagga gatagcaatg
     5941 acgttaccga gtggacaccc gaagagcaga ttgatcaaaa aatttaccgc actaggcccg
     6001 tatattcgtg aaggtaagtg cgaagataat cgattctttt tcgattgtct ggctgtatgc
     6061 gtcaacgtga aaccggcacc ggaagtgcgt gaattctggg gctggtggat ggagctggaa
     6121 gcgcaggaat cccgttttac ctacagctac cagtttggtc tgttcgataa agcaggcgac
     6181 tggaagagtg ttccggtaaa agatactgaa gtggttgaac gactggagca caccctgcgt
     6241 gagtttcacg agaagctgcg tgaactgctg acgacgctga atctgaagct ggaaccggcg
     6301 gatgattttc gtgatgaacc ggtgaagtta acggcgtgag tgaaatgtgc cggatgcatc
     6361 acatccggca atattcatta aaactgatac gtcatcccaa ccgcgacagt atcatcatta
     6421 ttaatattca gcttattatc gctatccagt ttgttgattt tataatcaac aaacgctgac
     6481 atatttttgt tgaaataata cgtagcaccg acgtcgatat aattgaccag atcttcatca
     6541 ccgatgccct caatatcttt ccctttcgat aagacataac ccagcgatgg acgcagacca
     6601 aagtcaaact ggtattgagc gaccgcttca aagttctgtg tcttattggc aaagccgcca
     6661 gttattggcg tcattttgcg tgtttcagaa tagaaagttg ccagataaat attattggca
     6721 tcgtatttca gacctgttgc ccatgcttct gcacgcttgc ctgtgccacg gctttgcagg
     6781 ttctgctcgt tggtgcgatc tgagttggta taggccccac taatggcgaa atcgctgccg
     6841 ccaaagtcat atgtcaatga cgtgccgaag ccatcgccgt tttgcttttt aacgtcgcgg
     6901 ttttcgtttt tcccttgata ttgcagggtt aagttcaggc catcgataac gccgaagaag
     6961 tcggtgttcc gatacgtcgc cagaccgctg gcgcgtttgg tcataaagtt gtcggtctgc
     7021 gccgaggaat cgccaccaaa ttccgggaac atatcggtcc aggcttccac gtcatacaac
     7081 gcgcccaggt tacgaccata atcgaaagaa cccaaatctt tatatttcaa cccggcaaaa
     7141 gcgagacgcg ttttttgctg tgcagtatca ctctccgctt tatttccggc aaactccgct
     7201 tcccaccgac cataaccagt cagttgatcg ttaatttgag tttcaccttt aaaaccaaaa
     7261 cgg
//
LOCUS       DAJTYF010000402         5653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.guided.185, whole genome shotgun sequence.
ACCESSION   DAJTYF010000402 DAJTYF010000000
VERSION     DAJTYF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.guided.185"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            575..1390
                     /gene="sul2"
                     /locus_tag="OO590_004428"
     CDS             575..1390
                     /gene="sul2"
                     /locus_tag="OO590_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6639499.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1477..1779
                     /locus_tag="OO590_004429"
     CDS             1477..1779
                     /locus_tag="OO590_004429"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639500.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(1955..3448)
                     /locus_tag="OO590_004430"
     CDS             complement(1955..3448)
                     /locus_tag="OO590_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCR6639501.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(3492..3881)
                     /locus_tag="OO590_004431"
     CDS             complement(3492..3881)
                     /locus_tag="OO590_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639502.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4099..4539
                     /locus_tag="OO590_004432"
     CDS             4099..4539
                     /locus_tag="OO590_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6639503.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5273..5422)
                     /locus_tag="OO590_004433"
     CDS             complement(5273..5422)
                     /locus_tag="OO590_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6639504.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1355 a         1543 c         1483 g         1272 t
ORIGIN      
        1 taactatcaa cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat
       61 tagtaacagg agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg
      121 gctacactga aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag
      181 cagttcccca actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta
      241 cagagcaaga gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta
      301 tcaattaatc ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa
      361 caggtttccc tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct
      421 acttattgag agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg
      481 atcaccgcaa acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga
      541 ctcatgctcg attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg
      601 catcgtcaac ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc
      661 agccattgcg caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc
      721 ggcatccagc aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc
      781 gccggtgctg gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc
      841 cgcgacgcaa gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt
      901 tccagacgct gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca
      961 ttcggtgcaa gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca
     1021 cattgcggcg ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa
     1081 ccgccttgtc cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct
     1141 ctcggtgctg gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc
     1201 tgtttcgcgc aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc
     1261 cgcgacactc gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca
     1321 cgagccgcgc cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag
     1381 aattcgttaa ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc
     1441 ctttgagggc gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca
     1501 gacggtattc gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc
     1561 ggcatggcgg ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc
     1621 aaggatacgc gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg
     1681 atgggcatgg acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc
     1741 ccttttcacc caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat
     1801 gcaacccaat ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca
     1861 ctagcggctc cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca
     1921 gcattttgat tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct
     1981 cagccggtgg cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct
     2041 tcaggtgatc aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca
     2101 tggcgccgcc gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt
     2161 gcgcccatgt catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga
     2221 ccttgttgcc cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg
     2281 cgaacacccc gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc
     2341 ttgcaatgaa atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct
     2401 tgagctggta gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg
     2461 ggcggctgat gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca
     2521 cgccggcgtg caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac
     2581 tggtcggcag agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac
     2641 ggtaagtgat cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg
     2701 ccagatagct gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca
     2761 cccggtgggc gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc
     2821 agcggaaacg cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt
     2881 ggaagtgaac attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg
     2941 tcttggccac ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt
     3001 aaacgatgcc cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct
     3061 gaaacgggaa gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca
     3121 gcaaggcggc actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct
     3181 tacagctgaa agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc
     3241 catgctccag ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca
     3301 attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct
     3361 gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc
     3421 tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata
     3481 cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga
     3541 cattgccagc cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg
     3601 ttattgcgtg caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa
     3661 ttaatattct tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat
     3721 gcagtataag atgaaccact actattatca tggccaccat taccaccacc gttaaaacta
     3781 cctcccgaac ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga
     3841 ccaaaaccac cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact
     3901 tttaaaaaca gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact
     3961 acgcccgtca aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca
     4021 gcctcagacc ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca
     4081 ggagacactg tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga
     4141 aagtcgcgct cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga
     4201 aaaaatgatc gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc
     4261 acttctgaga caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg
     4321 gaaaatgctc agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc
     4381 ctaatgcttg aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg
     4441 gaacaactac aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg
     4501 ggattctggt cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt
     4561 accataacat ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac
     4621 cggtaaaaca ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta
     4681 cggcatgcat gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg
     4741 taagtcctgt ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg
     4801 cgtggccgtg aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg
     4861 gggtgatgta caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa
     4921 aagtgatgta aaagtgatgt atgaactgaa ggagcgatgt aattctgtag tcaggaagaa
     4981 taacccgttc atgcaggcat gaacgataca gtgaggaacg ctttttttgt cacgatctcc
     5041 ccttaacctg cgtaaatcac ccgccatggt tgattatttt tcaacctcaa aatggtcata
     5101 cacctaacgc agccgcgatt ttcatcgcaa ctgcatgaat taaaagaaaa aaaaggaata
     5161 tttcgttttc acgagtaata cccgggcatt aaaaatccac tgtaattaca tgaaaataaa
     5221 gaaataaaag gtaataccgg agtattacca cgtacgcggg caagcgaaaa agctataacg
     5281 cttcccggcc gacaggcgac ccaaaatcca caggggcatg caggttctct tcagtaacct
     5341 gcgccattaa atcaaaaagg tcatattcgc cgggaagctg cgtatcagtc gaatccagtt
     5401 gttcatcata ttcagatggc atggggtgat atccagtagc tgagaggttg ttagcgggtt
     5461 tcggggcgca gccctgaacc agtcatgtag cgctagcgga gtgtatactg gcttactatg
     5521 ttgccacgat tgcgaaaatc agtgaagtgc ttcatgtggc aggagaaaaa aggcagcacc
     5581 ggcgcgtcag cagaatatgc gatacaggag atattccgct tcctcgctca ctgactcgct
     5641 acgctcggtc gtt
//
LOCUS       DAJTYF010000403         4874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.guided.202, whole genome shotgun sequence.
ACCESSION   DAJTYF010000403 DAJTYF010000000
VERSION     DAJTYF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.guided.202"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..1396
                     /gene="frdA"
                     /locus_tag="OO590_004434"
     CDS             <1..1396
                     /gene="frdA"
                     /locus_tag="OO590_004434"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6639505.1"
                     /translation="TLFQTSLQFPQIQRFDEHFVLDILVDDGHVRGLVAMNMMEGTLV
                     QIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMALSHGVPLRDMEFVQYHPTGLPGS
                     GILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLGEPKNKYMELGPRDKVSQAFWHE
                     WRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICELAKAYVGVDPVKEPIPVRPTAH
                     YTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRLGSNSLAELVVFGRLAGEQATER
                     AATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWAKIRDEMGLAMEEGCGIYRTPEL
                     MQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIELSHGLNVAECMAHSAMARKESR
                     GAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAA
                     DKAEAANNKEKANG"
     gene            1389..2123
                     /gene="frdB"
                     /locus_tag="OO590_004435"
     CDS             1389..2123
                     /gene="frdB"
                     /locus_tag="OO590_004435"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6639506.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2134..2529
                     /gene="frdC"
                     /locus_tag="OO590_004436"
     CDS             2134..2529
                     /gene="frdC"
                     /locus_tag="OO590_004436"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6639507.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            2540..2899
                     /gene="frdD"
                     /locus_tag="OO590_004437"
     CDS             2540..2899
                     /gene="frdD"
                     /locus_tag="OO590_004437"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6639508.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2962..4095
                     /locus_tag="OO590_004438"
     CDS             2962..4095
                     /locus_tag="OO590_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6639509.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            4184..4717
                     /gene="blc"
                     /locus_tag="OO590_004439"
     CDS             4184..4717
                     /gene="blc"
                     /locus_tag="OO590_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6639510.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4714..4854)
                     /gene="sugE"
                     /locus_tag="OO590_004440"
                     /pseudo
     CDS             complement(4714..4854)
                     /gene="sugE"
                     /locus_tag="OO590_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
BASE COUNT         1177 a         1278 c         1315 g         1104 t
ORIGIN      
        1 cacgctgttc cagacctctc tgcaattccc gcagatccag cgttttgacg aacatttcgt
       61 gctggatatt ctggttgatg atggtcatgt tcgcggcctg gtagcaatga acatgatgga
      121 aggcacgttg gtgcagatcc gtgctaacgc ggtcgttatg gctaccggcg gtgcgggtcg
      181 cgtttatcgt tacaacacca acggcggcat cgttaccggt gacggtatgg gtatggcgct
      241 aagccacggc gttccgctgc gtgacatgga attcgttcag tatcacccaa ccggtctgcc
      301 aggttccggt atcctgatga ccgaaggctg ccgtggtgaa ggcggtattc tggtcaacaa
      361 aaatggctac cgttatctgc aagattacgg catgggcccg gaaactccgc tgggcgagcc
      421 gaaaaacaaa tatatggaac tgggtccacg cgacaaagtt tctcaggcct tctggcacga
      481 atggcgtaaa ggcaacacca tctccacgcc gcgtggtgat gtggtttatc tcgacctgcg
      541 tcacctcggc gagaaaaaac tgcatgaacg tctgccgttc atctgcgaac tggcgaaagc
      601 gtacgttggc gtcgatccgg ttaaagaacc gattccggta cgtccgactg cacactacac
      661 catgggcggt atcgaaaccg atcagaactg tgaaacccgc attaaaggtc tgttcgccgt
      721 gggtgaatgt tcctctgttg gtctgcacgg tgcaaaccgt ctgggctcca actccctggc
      781 ggaactggtg gtcttcggcc gtctggccgg tgaacaagcg acagagcgtg cagcaactgc
      841 cggtaatggc aacgaagcgg caattgaagc gcaggcagct ggcgttgaac aacgtctgaa
      901 agatctggtt aaccaggatg gcggcgaaaa ctgggcgaag atccgcgacg aaatgggcct
      961 ggcaatggaa gaaggttgcg gtatctaccg tacgccggaa ctgatgcaga aaaccatcga
     1021 caagctggca gagctgcagg aacgcttcaa gcgcgtgcgc atcaccgaca cctccagcgt
     1081 gttcaacacc gacctgctct acaccattga actgagccac ggtctgaacg ttgctgaatg
     1141 tatggcgcac tccgcaatgg cacgtaaaga gtcccgcggc gcacaccagc gtctggacga
     1201 aggttgcacc gagcgtgacg acgtcaactt tctcaaacac accctcgcct tccgcgatgc
     1261 tgatggcacg actcgcctgg agtacagcga cgtgaagatt actacgctgc cgccagctaa
     1321 acgcgtttac ggtggcgaag cggatgcagc cgataaggcg gaagcagcca ataataagga
     1381 gaaggcgaat ggctgagatg aaaaacctga aaattgaggt ggtgcgctat aacccggaag
     1441 tcgataccgc accgcatagc gcattctatg aagtgcctta tgacgcaact acctcattac
     1501 tggatgcgct gggctacatc aaagacaacc tggcaccgga cctgagttac cgctggtcct
     1561 gccgtatggc gatttgtggt tcctgcggca tgatggttaa caacgtgcca aaactggcat
     1621 gtaaaacctt cctgcgtgat tacaccgacg gtatgaaggt tgaagcgtta gctaacttcc
     1681 cgattgaacg cgatctggtg gtcgatatga cccacttcat cgaaagtctg gaagcgatca
     1741 aaccgtacat catcggcaac tcccgcaccg cggatcaggg tactaacatc cagaccccgg
     1801 cgcagatggc aaagtatcac cagttctccg gttgcatcaa ctgtggtctg tgctacgccg
     1861 cgtgcccgca gtttggcctg aacccagagt tcatcggtcc ggctgccatt acgctggcgc
     1921 atcgttataa cgaagatagc cgcgaccacg gtaagaagga gcgtatggcg cagttgaaca
     1981 gccagaacgg tgtatggagc tgtactttcg tgggctattg ctccgaagtc tgcccgaaac
     2041 acgtcgatcc ggctgcggcc attcagcagg gcaaagtaga aagttcgaaa gactttctta
     2101 tcgcgaccct gaaaccacgc taaggagtgc aacatgacga ctaaacgtaa accgtatgta
     2161 cggccaatga cgtccacctg gtggaaaaaa ttgccgtttt atcgctttta catgctgcgc
     2221 gaaggcacgg cggttccggc tgtgtggttc agcattgaac tgattttcgg gctgtttgcc
     2281 ctgaaaaatg gcccggaagc ctgggcggga ttcgtcgact ttttacaaaa cccggttatc
     2341 gtgatcatta acctgatcac tctgacggca gctctgctgc acaccaaaac ctggtttgaa
     2401 ctggcaccga aagcggccaa tatcattgta aaagacgaaa aaatgggacc agagccaatt
     2461 atcaaaagtc tctgggcggt aactgtggtt gccaccatcg taatcctgtt tgttgccctg
     2521 tactggtaag gagcctgaga tgattaatcc aaatccaaag cgttctgacg aaccggtatt
     2581 ctggggcctc ttcggggccg gtggtatgtg gagcgcaatt attgcgccgg tgatgatcct
     2641 gctggtgggt attctgctgc cactggggct gtttccgggt gatgcgctga gctacgagcg
     2701 cgttctggcg ttcgcgcaga gcttcattgg tcgcgtattc ctgttcctga tgatcgttct
     2761 gccgctgtgg tgtggtttac accgtatgca ccacgcgatg cacgatctga aaatccacgt
     2821 gcctgcgggc aaatgggttt tctacggtct ggctgctatc ctgacagttg tcacgctgat
     2881 tggtgtcgtt acaatctaac gcatcgccaa tgtaaatccg gcccgcctat ggcgggccgt
     2941 tttgtatgga aaccagacct tatgttcaaa acgacgctct gcgccttatt aattaccgcc
     3001 tcttgctcca catttgctgc ccctcaacaa atcaacgata ttgtgcatcg cacaattacc
     3061 ccgcttatag agcaacaaaa gatccccggt atggcggtgg cggtaattta tcagggtaaa
     3121 ccttattact ttacttgggg ctatgcggac atcgccaaaa agcagcccgt cacacagcaa
     3181 acgttgtttg agttaggttc ggtcagcaaa acatttacgg gcgtgcttgg tggcgacgct
     3241 attgctcgag gggaaatcaa gttaagcgat cccgcaacaa aatactggcc tgaacttacc
     3301 gctaaacagt ggaatgggat cacactatta catcttgcga cctacaccgc tggcggcctg
     3361 ccattgcagg tgccggatga agtgaaatcc tcaagcgact tgctgcgctt ctatcaaaac
     3421 tggcagcctg catgggcccc aggaacacaa cgtctgtatg ccaactccag tatcggtttg
     3481 ttcggcgcac tggctgtgaa gccgtctggt ttgagttttg agcaggcgat gcaaactcgt
     3541 gtcttccagc cactcaaact caaccatacg tggattaatg tacctccccc agaagaaaag
     3601 aattacgcct ggggatatcg cgaaggtaag gcagtgcatg tttcgccagg ggcgttagat
     3661 gctgaagctt atggtgtgaa gtcgaccatt gaagatatgg cccgctgggt gcaaagcaat
     3721 ttaccccccc ttgatatcaa tgagaaaacg cttcaacaag ggatacaact ggcacaatct
     3781 cgctactggc aaaccggcga tatgtatcag ggcctgggct gggaaatgct ggactggccg
     3841 gtcaatcctg acagcatcat taacggcagt gacaataaaa ttgcactggc agcacgcccg
     3901 gtaaaagcga ttaagccccc aactcctgca gtacgcgcat catgggtaca taaaacgggg
     3961 gcgaccggcg gatttggtag ctatgtcgcg tttattccag aaaaagagct gggtatcgtg
     4021 atgctggcta acaaaaacta tcccaatcca gagagagtcg ccgccgcctg tcagattctc
     4081 aacgctctac agtaaaattt catcgggtcc gaattttcgg accttttctg cgcttttcct
     4141 tgctgtcatc tacacttaga aaaaaaccag taaggaaaca tttatgcgcc tgctccctct
     4201 cgttgccgca gcgacagctg catttctggt cgttgcctgt agttctccta cgccgccgcg
     4261 tggcgtgacc gtagtgaata atttcgacgc caaacgttat ctcggtacct ggtatgagat
     4321 tgcccgtttt gatcaccgtt ttgaacgtgg actggaaaaa gtcaccgcaa catacagcct
     4381 gcgtgatgac ggcggcctga atgtcattaa taaaggctat aaccctgaca gaggaatgtg
     4441 gcagcagagt gaagggaaag cgtactttac cggcgcacca actcgcgctg cgctgaaagt
     4501 gtcattcttt ggtcctttct atggcggtta taacgttatt gcactcgatc gggaataccg
     4561 ccatgcgctg gtttgcggcc cggaccgcga ctacctgtgg atactctccc gcacgccaac
     4621 catttctgac gaagtgaaac aggagatgct ggcagtcgcg acccgggaag ggtttgatgt
     4681 cagtaagttt atttgggtac agcagcccgg tagttagtga gtgctgagtt tcagaccaat
     4741 aatccccaat acgattaacg ccagactcgc cagacgcatc gggttagcgg actcaccgag
     4801 cagcacaatg ccggtgatgg ccgcgccgac tgcgccaata cccgtccaca cggcatcaaa
     4861 cggctaaagc cgtg
//
LOCUS       DAJTYF010000404         2895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.guided.235, whole genome shotgun sequence.
ACCESSION   DAJTYF010000404 DAJTYF010000000
VERSION     DAJTYF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.guided.235"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..82
                     /locus_tag="OO590_004441"
     CDS             <1..82
                     /locus_tag="OO590_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6639511.1"
                     /translation="LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(60..683)
                     /gene="tetR(B)"
                     /locus_tag="OO590_004442"
     CDS             complement(60..683)
                     /gene="tetR(B)"
                     /locus_tag="OO590_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6639512.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            765..1970
                     /gene="tet(B)"
                     /locus_tag="OO590_004443"
     CDS             765..1970
                     /gene="tet(B)"
                     /locus_tag="OO590_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6639513.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLASYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2083..2676)
                     /gene="tetC"
                     /locus_tag="OO590_004444"
     CDS             complement(2083..2676)
                     /gene="tetC"
                     /locus_tag="OO590_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6639514.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          779 a          537 c          582 g          997 t
ORIGIN      
        1 gttaactcga catcttggtt accgtgaagt taccatcacg gaaaaaggtt atgctgcttt
       61 taagacccac tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg
      121 aataagaagg ctggctctgc accttggtga tcaaataatt cgatagcttg tcgtaataat
      181 ggcggcatac tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttga
      241 tcttccaata cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata taatgcattc
      301 tctagtgaaa aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt
      361 ttttctgtag gccgtgtacc taaatgtact tttgctccat cgcgatgact tagtaaagca
      421 catctaaaac ttttagcgtt attacgtaaa aaatcttgcc agctttcccc ttctaaaggg
      481 caaaagtgag tatggtgcct atctaacatc tcaatggcta aggcgtcgag caaagcccgc
      541 ttatttttta catgccaata caatgtaggc tgctctacac ctagcttctg ggcgagttta
      601 cgggttgtta aaccttcgat tccgacctca ttaagcagct ctaatgcgct gttaatcact
      661 ttacttttat ctaatctaga catcattaat tcctaatttt tgttgacact ctatcattga
      721 tagagttatt ttaccactcc ctatcagtga tagagaaaag tgaaatgaat agttcgacaa
      781 agatcgcatt ggtaattacg ttactcgatg ccatggggat tggccttatc atgccagtct
      841 tgccaacgtt attacgtgaa tttattgctt cggaagatat cgctaaccac tttggcgtat
      901 tgcttgcact ttatgcgtta atgcaggtta tctttgctcc ttggcttgga aaaatgtctg
      961 accgatttgg tcggcgccca gtgctgttgt tgtcattaat aggcgcatcg ctggattact
     1021 tattgctggc tttttcaagt gcgctttgga tgctgtattt aggccgtttg ctttcaggga
     1081 tcacaggagc tactggggct gtcgcggcat cggtcattgc cgataccacc tcagcttctc
     1141 aacgcgtgaa gtggttcggt tggttagggg caagttttgg gcttggttta atagcggggc
     1201 ctattattgg tggttttgca ggagagattt caccgcatag tccctttttt atcgctgcgt
     1261 tgctaaatat tgtcgctttc cttgtggtta tgttttggtt ccgtgaaacc aaaaatacac
     1321 gtgataatac agataccgaa gtaggggttg agacgcaatc gaattcggta tacatcactt
     1381 tatttaaaac gatgcccatt ttgttgatta tttatttttc agcgcaattg ataggccaaa
     1441 ttcccgcaac ggtgtgggtg ctatttaccg aaaatcgttt tggatggaat agcatgatgg
     1501 ttggcttttc attagcgggt cttggtcttt tacactcagt attccaagcc tttgtggcag
     1561 gaagaatagc cactaaatgg ggcgaaaaaa cggcagtact gctcggattt attgcagata
     1621 gtagtgcatt tgccttttta gcgtttatat ctgaaggttg gttagttttc cctgttttaa
     1681 ttttattggc tggtggtggg atcgctttac ctgcattaca gggagtgatg tctatccaaa
     1741 caaagagtca tcagcaaggt gctttacagg gattattggt gagccttacc aatgcaaccg
     1801 gtgttattgg cccattactg tttgctgtta tttataatca ttcactacca atttgggatg
     1861 gctggatttg gattattggt ttagcgtctt actgtattat tatcctgcta tcgatgacct
     1921 tcatgttaac ccctcaagct caggggagta aacaggagac aagtgcttag ttatttcgtc
     1981 accaaatgat gttattccgc gaaatataat gaccctcttg ataacccaag agggcatttt
     2041 ttacgataaa gaagatttag cttcaaataa aacctatcta ttttatttat ctttcaagct
     2101 caataaaaag ccgcggtaaa tagcaataaa ttggcctttt ttatcggcaa gctcttttag
     2161 gtttttcgca tgtattgcga tatgcataaa ccagccattg agtaagtttt taagcacatc
     2221 atcatcataa gctttaagtt ggttctcttg gatcaatttg ctgacaatgg cgtttacctt
     2281 accagtaatg tattcaaggc taattttttc aagttcattc caaccaatga taggcatcac
     2341 ttcttggata gggataaggt ttttattatt atcaataata taatcaagat aatgttcaaa
     2401 tatactttct aaggcagacc aaccatttgt taaatcagtt tttgttgtga tgtaggcatc
     2461 aatcataatt aattgctgct tataacaggc actgagtaat tgttttttat ttttaaagtg
     2521 atgataaaag gcacctttgg tcaccaacgc ttttcccgag atctcatcta ttgaaacagc
     2581 ttgatagcct ttttcaacaa acaatattcg tgctgagtta accagtgatt gataggtact
     2641 cttaaaattt tcttgttgat gatttttatt ttccatgata gatttaaaat aacataccgt
     2701 cagtatgttt atggtatcat gatgatgtgg tcgtgacaat cttaagaaca tttaggttat
     2761 tttatgtata ttgaacagca ttctcgctat caaaataaag ctaataacat ccaattaaga
     2821 tatgatgata agcagtttca tacaacggtt atcaaagatg ttctattatg gattgaacat
     2881 aatttagatc agtct
//
LOCUS       DAJTYF010000405         2895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.guided.236, whole genome shotgun sequence.
ACCESSION   DAJTYF010000405 DAJTYF010000000
VERSION     DAJTYF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.guided.236"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            <1..82
                     /locus_tag="OO590_004445"
     CDS             <1..82
                     /locus_tag="OO590_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6639515.1"
                     /translation="LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(60..683)
                     /gene="tetR(B)"
                     /locus_tag="OO590_004446"
     CDS             complement(60..683)
                     /gene="tetR(B)"
                     /locus_tag="OO590_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6639516.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            765..1970
                     /gene="tet(B)"
                     /locus_tag="OO590_004447"
     CDS             765..1970
                     /gene="tet(B)"
                     /locus_tag="OO590_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6639517.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2083..2676)
                     /gene="tetC"
                     /locus_tag="OO590_004448"
     CDS             complement(2083..2676)
                     /gene="tetC"
                     /locus_tag="OO590_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6639518.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          779 a          536 c          582 g          998 t
ORIGIN      
        1 gttaactcga catcttggtt accgtgaagt taccatcacg gaaaaaggtt atgctgcttt
       61 taagacccac tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg
      121 aataagaagg ctggctctgc accttggtga tcaaataatt cgatagcttg tcgtaataat
      181 ggcggcatac tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttga
      241 tcttccaata cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata taatgcattc
      301 tctagtgaaa aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt
      361 ttttctgtag gccgtgtacc taaatgtact tttgctccat cgcgatgact tagtaaagca
      421 catctaaaac ttttagcgtt attacgtaaa aaatcttgcc agctttcccc ttctaaaggg
      481 caaaagtgag tatggtgcct atctaacatc tcaatggcta aggcgtcgag caaagcccgc
      541 ttatttttta catgccaata caatgtaggc tgctctacac ctagcttctg ggcgagttta
      601 cgggttgtta aaccttcgat tccgacctca ttaagcagct ctaatgcgct gttaatcact
      661 ttacttttat ctaatctaga catcattaat tcctaatttt tgttgacact ctatcattga
      721 tagagttatt ttaccactcc ctatcagtga tagagaaaag tgaaatgaat agttcgacaa
      781 agatcgcatt ggtaattacg ttactcgatg ccatggggat tggccttatc atgccagtct
      841 tgccaacgtt attacgtgaa tttattgctt cggaagatat cgctaaccac tttggcgtat
      901 tgcttgcact ttatgcgtta atgcaggtta tctttgctcc ttggcttgga aaaatgtctg
      961 accgatttgg tcggcgccca gtgctgttgt tgtcattaat aggcgcatcg ctggattact
     1021 tattgctggc tttttcaagt gcgctttgga tgctgtattt aggccgtttg ctttcaggga
     1081 tcacaggagc tactggggct gtcgcggcat cggtcattgc cgataccacc tcagcttctc
     1141 aacgcgtgaa gtggttcggt tggttagggg caagttttgg gcttggttta atagcggggc
     1201 ctattattgg tggttttgca ggagagattt caccgcatag tccctttttt atcgctgcgt
     1261 tgctaaatat tgtcgctttc cttgtggtta tgttttggtt ccgtgaaacc aaaaatacac
     1321 gtgataatac agataccgaa gtaggggttg agacgcaatc gaattcggta tacatcactt
     1381 tatttaaaac gatgcccatt ttgttgatta tttatttttc agcgcaattg ataggccaaa
     1441 ttcccgcaac ggtgtgggtg ctatttaccg aaaatcgttt tggatggaat agcatgatgg
     1501 ttggcttttc attagcgggt cttggtcttt tacactcagt attccaagcc tttgtggcag
     1561 gaagaatagc cactaaatgg ggcgaaaaaa cggcagtact gctcggattt attgcagata
     1621 gtagtgcatt tgccttttta gcgtttatat ctgaaggttg gttagttttc cctgttttaa
     1681 ttttattggc tggtggtggg atcgctttac ctgcattaca gggagtgatg tctatccaaa
     1741 caaagagtca tcagcaaggt gctttacagg gattattggt gagccttacc aatgcaaccg
     1801 gtgttattgg cccattactg tttgctgtta tttataatca ttcactacca atttgggatg
     1861 gctggatttg gattattggt ttagcgtttt actgtattat tatcctgcta tcgatgacct
     1921 tcatgttaac ccctcaagct caggggagta aacaggagac aagtgcttag ttatttcgtc
     1981 accaaatgat gttattccgc gaaatataat gaccctcttg ataacccaag agggcatttt
     2041 ttacgataaa gaagatttag cttcaaataa aacctatcta ttttatttat ctttcaagct
     2101 caataaaaag ccgcggtaaa tagcaataaa ttggcctttt ttatcggcaa gctcttttag
     2161 gtttttcgca tgtattgcga tatgcataaa ccagccattg agtaagtttt taagcacatc
     2221 atcatcataa gctttaagtt ggttctcttg gatcaatttg ctgacaatgg cgtttacctt
     2281 accagtaatg tattcaaggc taattttttc aagttcattc caaccaatga taggcatcac
     2341 ttcttggata gggataaggt ttttattatt atcaataata taatcaagat aatgttcaaa
     2401 tatactttct aaggcagacc aaccatttgt taaatcagtt tttgttgtga tgtaggcatc
     2461 aatcataatt aattgctgct tataacaggc actgagtaat tgttttttat ttttaaagtg
     2521 atgataaaag gcacctttgg tcaccaacgc ttttcccgag atctcatcta ttgaaacagc
     2581 ttgatagcct ttttcaacaa acaatattcg tgctgagtta accagtgatt gataggtact
     2641 cttaaaattt tcttgttgat gatttttatt ttccatgata gatttaaaat aacataccgt
     2701 cagtatgttt atggtatcat gatgatgtgg tcgtgacaat cttaagaaca tttaggttat
     2761 tttatgtata ttgaacagca ttctcgctat caaaataaag ctaataacat ccaattaaga
     2821 tatgatgata agcagtttca tacaacggtt atcaaagatg ttctattatg gattgaacat
     2881 aatttagatc agtct
//
LOCUS       DAJTYF010000406         2292 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.guided.245, whole genome shotgun sequence.
ACCESSION   DAJTYF010000406 DAJTYF010000000
VERSION     DAJTYF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.guided.245"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            263..922
                     /locus_tag="OO590_004449"
     CDS             263..922
                     /locus_tag="OO590_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6639519.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="OO590_004450"
     CDS             complement(1123..1500)
                     /locus_tag="OO590_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6639520.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..>2292)
                     /locus_tag="OO590_004451"
     CDS             complement(1567..>2292)
                     /locus_tag="OO590_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR6639521.1"
                     /translation="EEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDA
                     AYDALKPMIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVA
                     LRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQ
                     QRYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGD
                     YLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          546 a          578 c          614 g          554 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tc
//
LOCUS       DAJTYF010000407         1494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504056
            SAMEA8900207-rid15816213.guided.284, whole genome shotgun sequence.
ACCESSION   DAJTYF010000407 DAJTYF010000000
VERSION     DAJTYF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900207
            Sequence Read Archive: ERR6005076
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477990.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477990.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:48:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,380
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 381
            CDSs (without protein)            :: 381
            Pseudo Genes (ambiguous residues) :: 0 of 381
            Pseudo Genes (frameshifted)       :: 194 of 381
            Pseudo Genes (incomplete)         :: 102 of 381
            Pseudo Genes (internal stop)      :: 129 of 381
            Pseudo Genes (multiple problems)  :: 41 of 381
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900207-rid15816213.guided.284"
                     /serovar="S. flexneri 3a"
                     /isolate="504056"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-16"
     gene            17..490
                     /locus_tag="OO590_004452"
     CDS             17..490
                     /locus_tag="OO590_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6639522.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            583..1374
                     /gene="aadA1"
                     /locus_tag="OO590_004453"
     CDS             583..1374
                     /gene="aadA1"
                     /locus_tag="OO590_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6639523.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          405 a          345 c          379 g          365 t
ORIGIN      
        1 cctctgagga agaattgtga aactatcact aatggtagct atatcgaaga atggagttat
       61 cgggaatggc cctgatattc catggagtgc caaaggtgaa cagctcctgt ttaaagctat
      121 tacctataac caatggctgt tggttggacg caagactttt gaatcaatgg gagcattacc
      181 caaccgaaag tatgcggtcg taacacgttc aagttttaca tctgacaatg agaacgtagt
      241 gatctttcca tcaattaaag atgctttaac caacctaaag aaaataacgg atcatgtcat
      301 tgtttcaggt ggtggggaga tatacaaaag cctgatcgat caagtagata cactacatat
      361 atctacaata gacatcgagc cggaaggtga tgtttacttt cctgaaatcc ccagcaattt
      421 taggccagtt tttacccaag acttcgcctc taacataaat tatagttacc aaatctggca
      481 aaagggttaa caagtggcag caacggattc gcaaacctgt cacgcctttt gtaccaaaag
      541 ccgcgccagg tttgcgatcc gctgtgccag gcgttaaaca tcatgaggga agcggtgatc
      601 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
      661 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
      721 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
      781 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
      841 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
      901 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
      961 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     1021 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     1081 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     1141 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     1201 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     1261 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     1321 gaagaatttg ttcactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     1381 acaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact
     1441 aagcacataa ttgcggtgat gctgccaact tactgattta gtgtatgatg gtgt
//
